LOCUS       DAOMGY010000001        12475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_5043_length_12475_cov_4.842110, whole genome shotgun sequence.
ACCESSION   DAOMGY010000001 DAOMGY010000000
VERSION     DAOMGY010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12475
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5043_length_12475_cov_4.842110"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLR57_00005"
     CDS             <1..336
                     /locus_tag="PLR57_00005"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ39865.1"
                     /translation="AYVADESCGFPFTAHAMYDVFTGLTEISNEKWAKRVPKRPILVM
                     SGAKDPVGNAGKGPKQVYGWLKKTGHDVELKLYEDGRHEMLNEINRKDVYNDVLLFIN
                     AVEAMGELK"
     gene            333..1001
                     /locus_tag="PLR57_00010"
     CDS             333..1001
                     /locus_tag="PLR57_00010"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HQQ39866.1"
                     /translation="MSVLVYRDSDTLSQAVATLLTGCIIEKPASALGLDCSQRLAAVY
                     RKIAGMTSDGLLDWATIRAFNLYEHVKADSETTALSRMHAMLYDRVNIPPDSIFAPNT
                     DTRDWSVVCNDYEDSILGVGGLDTVVCVVEKDGRIACNMPAAELAPVTHVERTENGRV
                     VTVGISTIMSAKRVIAVLSGYELSQTAGLVINGPIVPMVPASYLQLHSNTIFMLDEDA
                     AEQI"
     gene            1024..1602
                     /locus_tag="PLR57_00015"
     CDS             1024..1602
                     /locus_tag="PLR57_00015"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQQ39867.1"
                     /translation="MAELSWESIECEIKDCRRCGLCKERKSIVLGEGNRSADIMFIGE
                     GPGGDEDRLGRPFVGKAGQLLDRMIAAIDMKREDLYITNVVKCRPPGNRTPLDEEAHA
                     CLPILRKQYALIKPKIVVCLGATSAKHVYSPEARITRDRGKWVEKNGVWFMPTYHPAA
                     LLRDESKKREVWDDLQSLAQKYRSIQLDGKGN"
     gene            1612..2349
                     /locus_tag="PLR57_00020"
     CDS             1612..2349
                     /locus_tag="PLR57_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQQ39868.1"
                     /translation="MSGHSKWANIKNKKEKTDSQRGRIFTKIGRELAIAVKEGGPDPV
                     NNNKLRDVIAKAKANNMPNDNIARSIKKAAGEGSSVNYEEITYEGYGPGNIAVIVSVL
                     TDNRNRTAAEMRHIFDKSGGNLGNSGCVAWMFDKKGLIVIERTAKMDEDEVMMQALEA
                     GAADFIAQDDAFEIYTDPLEFSKVREALEALKYEFISAEVQMIPQNTVTITDPESVEK
                     IERFLEKLDENEDVQEVFHNGILPEEE"
     gene            2493..2846
                     /locus_tag="PLR57_00025"
     CDS             2493..2846
                     /locus_tag="PLR57_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HQQ39869.1"
                     /translation="MAAEMSTSMGKIMIADDLIASIAGYAAVENYGIVGMIAKNAADP
                     FIELFGKDNMRRGVKVTILSPEVIDIDLYVTLEYGVSLPAVAQNVKSNVKYRVEEMTG
                     LTVHAVNVHVENIRV"
     gene            2886..4526
                     /locus_tag="PLR57_00030"
     CDS             2886..4526
                     /locus_tag="PLR57_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HQQ39870.1"
                     /translation="MDKNVTIGGAQLRDMIIAGAVLLEKNKAAIDALNVFPVPDGDTG
                     TNMSMTMQSAVREIRALPEDANATQVADALSMGALKGARGNSGVILSQLFRGFSRAFK
                     GAAIVDAELLSHALTMGSEAAYKAVMKPKEGTILTVSRVIAESVEEHQQQGANVYRLM
                     DVILEVGERALAETPKQLPVLKEAGVVDSGGKGLLTIYRGFKLSLDGEEVESYEELVS
                     DKPAEIRNEDSFSGENIVYGYCTEFFIIHLGPETNDAEIESFRNHLERLGDSVVVASD
                     AGMIKVHVHSNSPGKVLQMALRLGELEHLKIENMREQNRQLMEERKRNEKEFGLLAVS
                     SGEGIDELFRALAVDALISGGQTMNPSIDTIASAINKINARKVFVLPNNSNIILAAQQ
                     ASELTEKGVVLIPTKTIPQGIAACMAYSKDASVEENEAAMKGAIGDVVSGAVTYAVRD
                     TTIEGTKIKKGDIIGLINNHMAVSCASVEESTIALVRKMITEKGDSGSTVTLYYGEGQ
                     DENNAAAIADTLQGEFPDAEVVVLAGGQPLYYYYVAVQ"
     gene            4570..6732
                     /gene="recG"
                     /locus_tag="PLR57_00035"
     CDS             4570..6732
                     /gene="recG"
                     /locus_tag="PLR57_00035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQQ39871.1"
                     /translation="MRAEPSGSPQKVKESSVCKERERIWPTTEEAPLETSVRRSQNPP
                     PAIFVSKDMIDLPLTSVKGIGPKRTELFEQLGVRSARELLYRLPRDYLDYSSVTPVAA
                     LTDGKYAATQVRISSAPRYFRKGGMTVISASAQDETGCVTLKWFNQPYRSMQVKAGEV
                     VCVCGRVQRKKGVALLNPMITNVLPGILPVYATVKGLNQRTWRDSQMAALEAVWDRID
                     ETLPQKIIEQYELSPLALALRHAHFPFSREALELARKRLDFENALLYFIAVEEQKAER
                     SRQNGFAFDTSGVLEKFRAKLGYELTSAQRRVISEIAADMERPVPMNRLLQGDVGSGK
                     TAVALYALCVASANGKQGVLLAPTEILAEQHYETLRAIFGSSAALLTGSMKKAERDDV
                     LRRIADGTALCVTGTHALLSGDVKFYDLGCVVTDEQHRFGVRQRAAMQEKGVRPDMLV
                     MSATPIPRTLALILYGDLDISVIDELPPGRKPVKTSLIPPQKREDMYVYLSKQAVEQG
                     QQSYIVCPLIEESENIDCPSVSALFSELKKTLPKTRLCMLHGQMKDAEKERVMRAFRA
                     GEIDMLVTTTVIEVGVHVENACFMVIEGAERFGLSQLHQLRGRVGRSAMQAYCFLLTS
                     EGNGEANARIQALIKTSDGFEIARQDLMLRGPGDFIGVRQHGDAGLMMLGGVLDVQTL
                     ELAGRAAREIVSTPNVESASLVELANERYDAMSDIAMN"
     gene            6949..8127
                     /locus_tag="PLR57_00040"
     CDS             6949..8127
                     /locus_tag="PLR57_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQQ39872.1"
                     /translation="MNYQDIISDCVKRIPPSGIRKYFDLLDDRYISLGVGEPDFPTPE
                     RIRAYVTNKLKSERVPYTSNSGDPHLRELILRYLSDRYAISGYESIDNVLITVGASQA
                     IDLAMRAILNPGDEVIVHEPLYVAYRPSVELAGGKAVTVVTKHEDRFRLRAEAVKAAI
                     TPKTKAILLAFPNNPTGAIMEKADLEAIADVVRDTDIIVITDEIYAELTFNGKRHFSF
                     ASLPGMLERTIVLNGFSKAFSMTGWRIGYAAGQKDIIGAMRKVHQLTMLCAPTTAQWA
                     GVAALETGFSDDFADTRAMIDEYAVRRRFIIDAFNDMGLSCNEPEGAFYVFPSIRSTG
                     LTSEQFCDGLLASKKIVTVPGSAFGAAGEGHIRCCYATSMQDIETAMRMIAEYLTEIR
                     "
     gene            8239..10593
                     /locus_tag="PLR57_00045"
     CDS             8239..10593
                     /locus_tag="PLR57_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HQQ39873.1"
                     /translation="MIHEKTFTTLEYDKILSILKQHLACEVGAEFAEKLRPASDIKEV
                     ETLQEQTDEAEAIYSRTGRTPISGFPDVRELVGRMRAALFISARELLAVAAAMRASRE
                     AKEVLQAGDAESILCNMANRLSSHRSAEEEIARCILSEDEISDNASAELSRIRRQMKI
                     VNERVREKLNSMLKSQTTQKYLQEAIITVRNGRYALAVKAEHRAQVPGLVHDQSSSGA
                     TLFIEPAAVVELGNEYKRLLAEEKNEIERILSGLTAMLSPFADEIHMSVNVMGALDVI
                     FARAVMARDFRAVRPKLNDEGRIRILKGRHPLIPKDKVVPVDIWLGETFQTLIITGPN
                     TGGKTVTLKTVGLFSLMAMSGMFIPAEYGTELSTFENVFADIGDEQSIEQSLSTFSSH
                     MTNTVRILAQADDRSLVLLDELGAGTDPIEGAALAQAILEHLYEQHTLCVATTHYSEI
                     KAFALMHPGMQNASMEFDVDRLCPTYRLFIGIPGKSNAFEISRRLGLGGDVIDRAQTF
                     LKKKDVAFENVLSEAEQAKRTAENDRDEAKKALAEAEKIRSELAKQKQKLEDEKAQLR
                     QKAREDARRMVQETRQDMERLIAQLRSIENIDQKQLERAVQKTRDDMRATEERLYEQA
                     AQREAAGDAPHSVHPGERVLLLTLGQEATVVKAADSKGEVLVQAGVIKMSIPLKDLRP
                     VEVKKKPQSSSSKISISQDRGSALQIDLRGKMVDEACMELDRFIDNATMTGINEFFVV
                     HGKGTGALRTGVQAYLRNHPRVKTFRIGAYGEGDAGVTVVTLKA"
     gene            10762..11439
                     /locus_tag="PLR57_00050"
     CDS             10762..11439
                     /locus_tag="PLR57_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQQ39874.1"
                     /translation="MENNTILVIDDDRNILAIIELYLKKAGFQVYTCADGISALAAFH
                     ETKPSLVVLDIMLPGRDGWAVLHDIRMESDTPVIMLTAKGDTGDRVQGLELGADDYIS
                     KPFDAKELIARIKAVLRRSTPAEPERTISLPGLTVSLENYAVTLDGRQLEMPPKEIEL
                     LYFLASHTGKVFTREQLLEQVWGFDFFGESRTVDVHVKRIREKLGENHEWELRTVWGV
                     GYKFEQK"
     gene            11502..>12475
                     /locus_tag="PLR57_00055"
     CDS             11502..>12475
                     /locus_tag="PLR57_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQQ39875.1"
                     /translation="MTVTLGLLLLSGVVIGALMSGFVMREQKQDVANEIAVVSELYFN
                     TTAASVGENGAILELNTIARHYDALIQIISLDGTMAQYASGSKWDSIMSTRYTEEEIT
                     RMLNAVSDLDSSSVRFTQQKTTFQMLIGSRPLLEEGVATHVIRFYVDITEARGAVGMA
                     YMEVILVSLIAVLISVIVVYYTTTRLTRPFMEINEIVQKYSKGDYNVRIPISSIEEAT
                     QLAISFNTMADQLKDLEATRRSFVANVSHELRSPLTSMKGFLEAMQDGTIGPDEYEKY
                     IGIVLSETKRMAVMVNDLLDLARIESGKTALKLEIFDINELIRRTLIT"
BASE COUNT         2965 a         3440 c         3692 g         2378 t
ORIGIN      
        1 gcctacgttg ccgacgaaag ctgcggtttt ccgttcaccg cgcacgccat gtacgacgtg
       61 tttactggtt tgaccgaaat ctccaatgaa aagtgggcaa agcgcgtgcc caagcgcccg
      121 attcttgtga tgtccggcgc aaaggatcca gtcggcaacg ctggcaaagg ccccaagcaa
      181 gtctacggct ggctcaaaaa gaccgggcac gacgtggagc tcaagctcta cgaagacggc
      241 cgccatgaga tgctcaacga gatcaaccgc aaggatgttt ataacgatgt actgctgttc
      301 atcaacgctg tggaagcgat gggagagctg aaatgagcgt actggtctac cgggacagcg
      361 acacgcttag ccaggccgtg gctacgctgc tcaccggctg catcatagaa aaacccgcgt
      421 cggcgctggg gctggactgc tcgcagaggc tcgccgccgt ctaccgcaaa attgcgggca
      481 tgaccagcga cggcctgctc gactgggcga ccatccgcgc gttcaacctc tacgaacacg
      541 tcaaggcgga ttcggaaacg accgcgcttt cccgcatgca tgccatgctg tacgaccgtg
      601 tgaacatccc gccggacagc atttttgcgc caaatacgga cacgcgggac tggagcgtcg
      661 tctgcaacga ctatgaggat tccatccttg gcgtcggcgg actggatacg gttgtctgcg
      721 tcgtggaaaa agacggaaga atcgcctgca acatgcccgc cgccgaactg gcacccgtta
      781 cgcacgtgga gcgcacggag aacggccgcg tggtcaccgt tggcatttcg accatcatgt
      841 ccgcaaagcg cgttattgcc gttctctcgg ggtatgagct ttctcagacc gcggggcttg
      901 ttatcaacgg gccgatcgtg ccaatggtgc cagcaagcta cctgcagctg cattccaata
      961 ccattttcat gctggacgaa gacgctgccg agcagatata acaggttacg aacggagcgg
     1021 gcgatggcgg aactgagctg ggaatccatc gagtgtgaaa ttaaggactg tcgccgctgc
     1081 ggcctctgta aggagcgaaa aagtatcgtc ctcggagaag gaaaccgctc cgccgacatc
     1141 atgttcatcg gcgaaggacc cggcggggac gaagaccgtt tgggtcgtcc gttcgttgga
     1201 aaagcgggtc agcttctgga tcgaatgatc gcagccattg acatgaagcg cgaagacctg
     1261 tatattacca acgttgtcaa atgccgtccg ccgggaaacc gcacgccgct ggacgaagag
     1321 gcgcacgcct gtttgccgat ccttcgcaag cagtacgcgc tgatcaaacc gaagattgtc
     1381 gtatgccttg gagcgacttc cgcaaagcat gtttacagcc cggaagcgcg cattacccgc
     1441 gaccggggca agtgggtgga gaagaacggc gtatggttta tgccgacgta tcaccccgcg
     1501 gcactgctca gagacgagtc gaaaaaacgc gaagtctggg atgatctcca gtcgctcgcg
     1561 caaaaatacc ggtcgatcca actcgacggg aagggaaact aggaggaagt tatgtcagga
     1621 cattccaagt gggccaacat taaaaataaa aaggaaaaga cggattcgca gcgcggacgc
     1681 atctttacca agatcggcag agagctcgcc atcgccgtca aagagggcgg gcccgatccg
     1741 gtcaacaaca ataagctccg cgacgttatc gccaaggcga aagccaacaa catgcccaac
     1801 gataacatcg cccgctccat caaaaaggcc gccggcgaag gttcttccgt aaactacgaa
     1861 gaaatcactt acgaaggcta cggcccgggc aatatagcag taatcgtttc cgttctgacg
     1921 gataaccgta accgcaccgc cgcggagatg cggcatatct tcgacaagag cggcggcaac
     1981 ctcggcaaca gcggctgcgt tgcgtggatg ttcgacaaaa aaggcctcat cgtcattgag
     2041 cgcaccgcca agatggacga agacgaagtg atgatgcagg cgctggaagc gggcgctgcg
     2101 gactttattg cgcaggacga cgcgttcgaa atctataccg acccgctcga attctccaaa
     2161 gtgcgcgaag cgctggaagc gctcaagtac gaattcatct cggctgaagt acagatgatt
     2221 ccgcagaaca ccgtgaccat taccgacccg gagtcggttg agaaaatcga gcggtttttg
     2281 gaaaaactgg acgaaaatga ggacgttcag gaagtgttcc acaacggcat cctgcccgaa
     2341 gaagaataac acgacaggaa ttgcgttgtt tccaaatctt cacaggctgg ttacaataac
     2401 gccttgcgat tccccgcggg agactggtta cgcggttgcg ggtcaaaatt gcatggtata
     2461 aaatagaacc aaagacaaag gaggcgtaac ttatggcagc ggaaatgtct acatcgatgg
     2521 gaaagattat gattgcggac gatctgatcg ccagcatcgc cggatatgcg gccgtggaaa
     2581 actacggcat tgtcggcatg attgcgaaaa atgcggcaga cccgttcatt gaactgtttg
     2641 gaaaagataa catgcgccgc ggcgtcaagg ttacgattct ttcacccgaa gtgatcgata
     2701 tcgatctgta tgtaacgctc gagtacggcg tatccttgcc cgccgtagcg cagaacgtca
     2761 agagcaacgt taaataccgg gtggaagaaa tgacgggact gacggttcac gccgtcaacg
     2821 ttcacgttga aaacattcgc gtataacgca gttcggcctt gggttccgaa catacggagg
     2881 gaaaattgga taaaaacgta acgatcggcg gcgcacagct gcgcgatatg attatcgctg
     2941 gcgcagtgct gctggagaag aacaaagcgg caatcgacgc tttgaatgta ttccctgtcc
     3001 cggatgggga cacgggtacc aatatgtcca tgacgatgca gagcgcggta agggaaatcc
     3061 gcgcgctgcc ggaggacgca aacgcaacgc aagtggcgga tgcgctgagc atgggcgcgc
     3121 tcaagggcgc gcgcggcaac tccggggtta ttctttccca gctcttccgc gggttctcgc
     3181 gcgcgtttaa gggcgctgcc atcgttgatg cggagttgct ttcccacgcg ctgaccatgg
     3241 gaagcgaagc ggcttacaaa gccgttatga agcccaagga aggcacaatc ctcaccgtct
     3301 cccgcgtgat tgcggagagc gtggaggaac accagcagca gggagcgaat gtttaccggc
     3361 tgatggacgt tatccttgaa gtgggtgaaa gagcgctggc ggaaacgccc aagcagctgc
     3421 ccgtgctcaa ggaagcgggg gttgtggact ccggcggcaa gggactttta accatatacc
     3481 gcgggttcaa gctttcgctg gatggcgaag aagtggaaag ctacgaagag ctcgtgtccg
     3541 acaagcctgc ggaaattcgc aatgaggaca gcttttccgg cgaaaacatc gtttacggct
     3601 actgcacgga gttttttatc attcatcttg ggccggaaac gaatgacgcc gaaatcgaga
     3661 gcttccgcaa ccacctggaa cgccttggcg attccgttgt ggtcgccagc gacgcgggca
     3721 tgatcaaggt gcatgtgcac tccaactcgc ccggcaaggt gctgcagatg gcgctgcgcc
     3781 tcggcgaact ggaacacctc aaaattgaaa acatgcgcga gcagaaccgc cagctcatgg
     3841 aggagcgcaa gcgcaacgaa aaggaattcg gcctgctcgc cgtcagcagc ggagagggaa
     3901 ttgacgaact cttccgcgcg ctggccgttg acgcgctgat ctccggcgga cagacgatga
     3961 accccagcat cgatacaatc gcttccgcga tcaacaagat caacgcgcgc aaggtctttg
     4021 tgctgccgaa caactcaaac atcatcctcg cggcgcagca ggccagcgaa ctgaccgaaa
     4081 agggcgtcgt gctgattcct accaagacga ttccgcaggg aatcgccgcc tgcatggcct
     4141 actccaagga cgcttccgtc gaggaaaacg aagcggccat gaagggcgcc atcggggacg
     4201 tggtcagcgg cgcggtaacg tacgccgtgc gcgatacgac cattgaggga accaaaatca
     4261 agaaaggcga cattatcggc ctcatcaaca accatatggc cgtctcctgc gcaagcgtcg
     4321 aggaatcgac aatcgcgctc gttcgcaaga tgatcaccga aaagggcgac agcggttcca
     4381 ccgttacgct ctactacggc gaaggacagg acgaaaacaa cgccgccgcc attgcggaca
     4441 cgcttcaggg cgagttcccg gacgcggaag tcgtcgtgct cgcgggcggt cagccactct
     4501 actactacta cgttgccgta cagtaaaaag cggcgggttc tcagggctgc ctaagccctg
     4561 agcaggagta tgagggcgga gccctcaggg agccctcaga aagtaaagga atcttccgtt
     4621 tgtaaagaac gggaaaggat ttggccgacg acggaggagg cgccgctcga aacaagcgtt
     4681 cggcggagcc aaaatccgcc gccggccatc tttgtatcca aagacatgat cgatctcccg
     4741 ctgacaagcg taaaaggaat cggaccgaaa cgcacggagc tgtttgagca gctcggcgtg
     4801 cgttcggcgc gggaactgct gtaccgtttg ccgcgggatt atctggacta ttcttccgta
     4861 acgccggttg ctgcgctgac ggacggcaaa tacgcggcga cacaggtacg aatctcctcc
     4921 gcgccgcgtt acttccgcaa aggcggtatg acggtcatct ccgcctccgc acaggacgaa
     4981 acgggctgcg ttacgctcaa atggttcaat cagccgtacc gttccatgca ggtgaaagcg
     5041 ggcgaagtcg tctgcgtctg cgggcgtgtt cagcgcaaga aaggcgttgc gctgctcaac
     5101 ccgatgataa cgaacgtcct gcccggcatt ctgcccgtct acgcaacggt taaaggcttg
     5161 aaccagcgca cctggcggga cagccagatg gccgcattgg aagccgtttg ggatcgaatc
     5221 gacgaaacgc ttccgcaaaa gattatcgag cagtacgagc tttctccgct cgcgctggcg
     5281 ctgcggcacg cgcactttcc gttcagccgg gaagcgctgg aactggcgcg aaaacgcctg
     5341 gactttgaaa acgcgctgct gtatttcatc gctgttgaag agcagaaggc ggaacgctcg
     5401 cgtcagaacg gctttgcgtt cgatacgagc ggcgtactgg aaaagtttag agcaaaactc
     5461 ggctacgaac tgacgagcgc gcagagacgt gtgatcagcg aaatcgccgc cgatatggag
     5521 cggcctgtgc cgatgaaccg cctgctgcag ggggacgtcg gttccggcaa aacggctgtc
     5581 gcgctctacg cgctctgcgt ggcgagtgca aacggcaagc agggcgttct gctcgcgccg
     5641 acggagatcc tcgccgaaca gcactacgaa accctgcgcg cgatttttgg ttcttcggcg
     5701 gcgctgttga ccggcagcat gaaaaaagcg gagcgggacg acgtactgcg gcgcattgca
     5761 gacggcaccg cgctctgcgt gacgggcacg cacgcgctgc tctccggcga cgtaaagttt
     5821 tacgatctgg gttgtgtggt aacggacgaa cagcatcgct ttggcgtacg ccagcgcgcg
     5881 gccatgcagg aaaaaggcgt tcgcccggac atgctggtca tgagcgccac tcccattccg
     5941 cgtacgctcg cgctgattct gtacggggat ttggatatct ccgtgatcga cgagttgccg
     6001 cccgggcgga aacccgttaa aaccagtttg attccgccgc agaagcgcga ggacatgtat
     6061 gtttatctat ccaaacaagc ggtggagcag ggtcagcaga gctatatcgt ttgtccgttg
     6121 atcgaagaga gcgagaatat agattgtccc agcgtttccg cgctgttttc cgaactgaag
     6181 aagacgctgc caaagacgag gctctgcatg ctccacggtc agatgaagga tgctgaaaag
     6241 gagcgcgtga tgcgcgcgtt ccgcgcgggt gaaatcgaca tgctggtcac tacgacggtc
     6301 atagaagtcg gcgtgcatgt tgaaaacgcc tgctttatgg tgatcgaggg agcggagcgc
     6361 ttcggcttaa gccagctcca ccagctccgc gggcgcgtcg gcagaagcgc catgcaggct
     6421 tactgctttt tgctcacgag cgaggggaac ggggaagcca acgcgcggat tcaggcgctg
     6481 atcaagacca gcgacggctt tgaaatcgcg cggcaggatt taatgctgcg cgggccgggc
     6541 gattttatcg gcgtgcgtca gcatggcgac gcggggttga tgatgttggg cggcgtgctg
     6601 gacgtgcaaa cgctggagct ggcgggcaga gccgcgcggg agatcgtttc gacgccgaat
     6661 gtggagagcg caagtctcgt cgaactcgcc aacgaacgtt acgatgctat gagcgacatc
     6721 gccatgaatt agccgattcg agcctgttta ttctcggaac gctgtttatt ggaagcggca
     6781 aaaacggctg cgttttcgtt gcttttttaa tattattttg attcgtttga gaacgtgtgc
     6841 atctttatga gattttcgaa cacagaagga caattcgttt gacgcgtaca accctgcatg
     6901 ttatgatgtt cctacggctt tttgccgaac agacaaggac gtgtacgcat gaactatcag
     6961 gacatcatat cggactgcgt caaacgcata ccgccttccg gtattcgtaa atatttcgat
     7021 cttttggatg accgttatat cagcctcggc gttggcgagc ccgattttcc gacgccggag
     7081 cgcatccgcg cgtatgttac aaacaagctc aagagcgaac gcgtgcctta cacgtccaat
     7141 tccggcgatc cgcatctgcg ggagctgatt ctcaggtatc tcagcgaccg ctacgcaatc
     7201 tccggttatg aatcgatcga taacgtcctc attacggttg gcgcgagcca ggccatcgac
     7261 cttgccatgc gcgccatcct caaccccggc gacgaagtca tcgtccacga gccgctctac
     7321 gttgcctacc gtccttccgt cgaactggcg ggcggcaagg ctgttacggt cgtaacaaag
     7381 cacgaagaca ggtttcgcct gcgcgcggaa gcggtgaagg ccgccatcac gccaaagacg
     7441 aaggcgattc ttctcgcgtt cccgaacaac ccgaccggcg cgatcatgga aaaagccgat
     7501 ctggaagcga ttgcggacgt tgtgcgggat acggatatca tcgtcattac ggacgaaatc
     7561 tatgcggagc tgacgttcaa cggcaagcgc catttctcgt ttgcgtcgct gccgggcatg
     7621 ctggagcgca ccatcgtgct caacggcttc tccaaggcgt tttccatgac cggatggcgt
     7681 atcggctatg ccgcgggtca aaaggacatc atcggcgcca tgcggaaagt gcaccagctt
     7741 accatgcttt gcgcgccgac cacggcgcag tgggcgggcg ttgcggcgct ggaaacaggc
     7801 ttttcggacg attttgcgga cacccgcgcc atgatcgacg aatacgccgt gcgccgccgt
     7861 tttatcatcg acgcgttcaa cgacatgggg ctttcctgca atgagccgga aggcgcgttc
     7921 tatgtgttcc cgtcgattcg ttctacgggg ctgaccagtg agcagttctg cgacgggctg
     7981 cttgcaagca aaaagatcgt cacggtaccc ggcagcgcgt ttggcgcggc gggagagggg
     8041 catattcggt gctgctacgc gacctcgatg caggacatcg agaccgccat gcggatgatt
     8101 gcggagtatc taacggaaat ccgctgacaa acccgaagcc acgccccgtc cagacgataa
     8161 ggacggggct tttgtttgca aaaacgcgaa tttttgaaat catttgaaca atcacgacca
     8221 attttttagg agaacggcat gatacacgaa aaaacattta ccacgctgga atacgataaa
     8281 atactcagca tactgaaaca gcatctcgcc tgcgaagtcg gagccgaatt cgcggagaag
     8341 ctgcgccccg cttccgatat caaggaagtg gaaacgctgc aggaacaaac cgacgaagcg
     8401 gaagcgatct attcccgcac cggcagaacg cccatctccg gctttccgga cgtgcgcgag
     8461 ctggtcggcc gcatgcgcgc ggctctgttc atcagcgcgc gtgaactgct tgcggttgcc
     8521 gccgccatgc gggcctcccg cgaagcaaag gaagttctgc aggcggggga cgcggagagc
     8581 attctctgca acatggcaaa ccggctttcc tcccaccggt cagcggagga agaaatcgcc
     8641 cggtgcatcc tctcggagga tgaaatttcg gacaacgcgt ccgccgagct cagccgtatc
     8701 cgccggcaga tgaaaatcgt caacgaacgc gtgcgtgaaa agctcaactc catgctcaaa
     8761 agccagacca cgcagaagta cctgcaggaa gcgatcatca ccgtgcgcaa cggcagatac
     8821 gcgctcgccg tcaaggcgga gcaccgcgcg caggtgccgg gactggttca tgaccagagc
     8881 agcagcgggg ctacgctgtt tattgaaccc gcggcggtcg ttgaactggg caacgagtac
     8941 aagcggcttc tcgcggaaga gaaaaacgaa attgaacgca ttctttcggg gctgaccgcc
     9001 atgctctcgc cgtttgcgga cgaaatccat atgagcgtca acgtcatggg tgcgctggac
     9061 gtgatctttg cgcgcgcggt gatggcgcgg gatttccgcg ccgtgaggcc gaagctcaac
     9121 gatgagggca ggattcgcat cctcaaaggc aggcatccgc tgattccgaa ggacaaggtc
     9181 gttccggtcg atatctggct gggagagacg tttcaaacgc tgattatcac aggcccgaac
     9241 acgggcggta agaccgtaac gctcaaaacc gtcggcctgt tttcgctgat ggccatgagc
     9301 ggcatgttca tccctgctga gtacggaacg gagctctcga ccttcgagaa cgtctttgcc
     9361 gatatcgggg acgaacagtc catcgagcag tcgctttcca ccttctcgtc gcacatgacc
     9421 aataccgtcc gtatccttgc gcaagcggac gaccgctcgc tcgtgctgct ggacgaactt
     9481 ggcgcgggga ccgacccaat cgagggcgcg gcgctggcgc aggctatcct ggagcatctc
     9541 tacgaacagc acacgctctg cgtcgctacg acgcattaca gcgagatcaa ggcctttgcg
     9601 ctcatgcacc cgggcatgca gaacgcctcc atggagttcg acgtggatcg cctctgcccg
     9661 acctaccggc tctttatcgg cattcccggc aagtctaacg cgttcgaaat ctcccgcagg
     9721 ctcgggctcg gcggggatgt gatcgaccgc gcgcagacgt tcctgaaaaa gaaggacgtt
     9781 gcgttcgaaa acgtgctttc cgaagcggaa caggcaaaac gaaccgccga aaacgaccgc
     9841 gacgaagcga aaaaagcgct cgcggaagcg gagaagattc gttcggaact ggctaagcag
     9901 aagcaaaagc ttgaggacga aaaggcgcag ctccgccaga aggcgcgcga agacgcgcgg
     9961 cgcatggtgc aggagacgcg gcaggatatg gaacggctga ttgcgcagct tcgctccatt
    10021 gaaaacatcg accaaaagca gctggagcgc gcggtgcaga aaacacggga cgacatgcgc
    10081 gctaccgaag aacggcttta tgaacaggcc gcccagcggg aagcggcggg ggacgcgccg
    10141 cactctgtgc atcccggcga acgcgtgctg ctgctgacac tgggacagga agcgacggtc
    10201 gtcaaggcgg cggacagcaa gggcgaagtg ctggtgcagg cgggggtcat taaaatgagc
    10261 attccgctca aagatttgcg ccccgtggaa gtcaagaaga agccgcagtc gtcgtcatcc
    10321 aagatcagca tttcgcagga tcgcggcagc gcgctgcaaa tcgatctgcg cggcaaaatg
    10381 gtggacgaag cctgcatgga actcgaccgc tttatcgaca atgcgaccat gaccggcatc
    10441 aacgaattct tcgttgtgca cggcaaaggt acgggcgcgc tgcgcaccgg cgtgcaggcg
    10501 tatctgcgca accacccgcg cgtaaaaacc ttccgcatcg gcgcctatgg cgaaggcgac
    10561 gcgggggtca cggtagttac gctgaaagcc tgattcgggc atgaaaagct gaaatttcgg
    10621 gtaagatgcg ccgcactgtg caaacggtgc ggcgttcgtg tgacaaattg catgttggtt
    10681 ggacaaacga aaaaaactct gttatacttt acccaaatgt gagatatcgt atatttaacg
    10741 aactacaacg gaggtgatcg gatggagaac aacaccattc tggtcataga tgacgacaga
    10801 aatattctgg ctatcatcga gctgtattta aaaaaggccg gcttccaggt atacacctgc
    10861 gccgatggaa tttccgcgtt ggctgcgttt cacgaaacaa agccgtctct tgttgtgctc
    10921 gacattatgc ttcccggacg cgacggctgg gcggttctcc acgacattcg catggagagc
    10981 gatacgccgg tcatcatgct cacggcaaag ggcgatacgg gcgaccgcgt gcagggtctg
    11041 gagcttggtg cggacgacta tatttccaaa ccgttcgacg caaaggaact catcgcccgc
    11101 atcaaagcgg tgctccgacg gagcacgccc gccgaaccgg agcgcaccat cagcctgccg
    11161 ggtttgaccg tttcgctgga gaattacgcc gtcaccctcg acggtcgcca gctggaaatg
    11221 ccgcccaaag agattgaact gctgtatttt ctcgcctcgc ataccggtaa ggtgttcaca
    11281 cgcgaacagc tgctggaaca ggtttggggc tttgactttt ttggcgaatc ccgaaccgtg
    11341 gacgtccatg tgaaaagaat ccgcgaaaag ctcggggaga atcacgagtg ggaactccgc
    11401 accgtttggg gagtcgggta caaattcgag cagaagtgac gcgataacac atgcgcaagg
    11461 actttctacg aacgctattt tcccgtatgc tggcaacata catgactgtt acgctcggat
    11521 tgctcctgct ctccggcgtc gtcatcggcg cgctgatgtc cggctttgtc atgcgcgagc
    11581 agaagcagga cgtcgcaaat gaaatcgcgg tcgtttcgga actctacttt aacacaacgg
    11641 cagcctccgt cggcgagaac ggcgccatcc tcgaactcaa caccattgcc cgccactatg
    11701 acgcgctgat tcaaattatc tcgctcgacg gcacaatggc gcaatatgcc tccggttcca
    11761 agtgggactc cattatgagc acccgctaca ccgaggaaga aattacacgc atgctgaacg
    11821 cggtgtccga tctggactcg agcagcgtgc gttttacgca gcagaagacg acgtttcaaa
    11881 tgctcatcgg cagcaggccg ctgctggagg aaggcgttgc gacccacgta atccgctttt
    11941 atgtggatat tacggaagcg cgcggcgcgg tgggcatggc gtatatggaa gtcatactcg
    12001 tttcgctgat cgcggtgctg atttcggtga ttgtcgtata ctacaccacg acgcgcctga
    12061 caaggccgtt tatggagatc aacgaaatcg ttcaaaagta ctccaagggg gactacaacg
    12121 tccgtatccc gatcagcagc attgaggaag cgacgcagct ggccatcagc ttcaatacca
    12181 tggccgacca gctgaaagac ctggaagcca cgagaagaag cttcgtagcc aacgtcagcc
    12241 acgaactgcg ctccccgctg accagcatga aaggctttct ggaagccatg caggacggca
    12301 cgatcggccc cgacgagtat gaaaagtaca tcggcatcgt gctctcggaa accaaacgca
    12361 tggcggtaat ggtcaacgac ctgctcgacc ttgccagaat cgagtccggc aagactgcgc
    12421 tcaagctgga gatattcgat atcaacgagt tgatccgcag aaccctgatt acctt
//
LOCUS       DAOMGY010000002         8535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_8344_length_8535_cov_4.745755, whole genome shotgun sequence.
ACCESSION   DAOMGY010000002 DAOMGY010000000
VERSION     DAOMGY010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8344_length_8535_cov_4.745755"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLR57_00060"
     CDS             <1..346
                     /locus_tag="PLR57_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HQQ39876.1"
                     /translation="AALDSVFGFAPLGTPEPARRGGRPVMLVGPPAVGKTVTVAKLAA
                     ASDKLWARIIRAPGLGLQYLTTREPEQDMIEVAIAAFYLAMNKGKSDKLMPENDAEAE
                     PAQSETKEPDNT"
     gene            349..1182
                     /gene="prmC"
                     /locus_tag="PLR57_00065"
     CDS             349..1182
                     /gene="prmC"
                     /locus_tag="PLR57_00065"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQQ39877.1"
                     /translation="MTVAETVRLAKLRLEPVAGEESLQQAKLLVAAVIGAKADALMVH
                     TWMDVTEEQLVLICDLLERRLKGEPLQYILGEWEFMGLPFYVDERALIPRQDTELLCE
                     KALEIIRTQHSKSVLDLCTGSGCLAVSIAKLAETDLTVTASDVSTDALALAKENAILN
                     GVDASIGWIESDLFDKIPGTFDLIVCNPPYLTRPDMEHLQTEVAFEPRLALFGGEDGL
                     DIYRRIASEYKAHLNPGGTLLLEIGSTQAESVSALFGQTATVFNDLCGNPRVIQVQQS
                     E"
     gene            1191..2264
                     /gene="prfA"
                     /locus_tag="PLR57_00070"
     CDS             1191..2264
                     /gene="prfA"
                     /locus_tag="PLR57_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQQ39878.1"
                     /translation="MLEKLIGIKERYDLLSAKLAEPDAMADKDAWRAMVKERASLEEI
                     VLAFEEYQTAQESLENCRAMLNDHLDAEMKELVHAEIAELEEKAVSVEERLRVLLLPK
                     DPNDDKDVILEIRAGTGGDEASLFGADLLRMYLRYAERNSYKVEYLSSNMTDMGGVKE
                     VVLSISGKRGAYSRLKYESGVHRVQRVPETESQGRIHTSAATVAVLPEVDDIQIDIKE
                     SDLRIDTYRSSGAGGQHVNKTESAIRITHLPTGMVVSCQDEKSQLKNKEQAMRVLKSR
                     LFDFYQGQQDAERAGARKSQIGSGDRSERIRTYNFPQGRVTDHRIGVTLYRLEAFLDG
                     DMDELIDALILAERSQKLSGGTD"
     gene            2295..3341
                     /locus_tag="PLR57_00075"
     CDS             2295..3341
                     /locus_tag="PLR57_00075"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQQ39879.1"
                     /translation="MYQTQVINVERQPEAGSVQAAKLIQAGELVAFPTETVYGLGADG
                     LNGDAVQKIFEAKGRPADNPLIEHVAKRSDVRELWLKIPRVADTLMDAFWPGPLTLVA
                     QKSDHVPSEVTAGLNTVAVRMPLNKTARSLISKAGVPIAAPSANRSGRPSPTTAQHVL
                     EDMDGRIPLILDGGPCKYGVESTVLLLEGEPTILRPGAVTKEMLEAVIGEVQVAKSIL
                     QPMKQGEVAASPGMKYKHYAPNAEVVVVTGEPERVARTINALYRQAEAEGIDCEIAAT
                     EQTKNFYRGKKYVILGDRKSPETLCANLFSALREMDQSADRIFAEGVPAENEGLAYMN
                     RLLRSAGFKVIEAE"
     gene            3407..3856
                     /gene="rpiB"
                     /locus_tag="PLR57_00080"
     CDS             3407..3856
                     /gene="rpiB"
                     /locus_tag="PLR57_00080"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006040086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HQQ39880.1"
                     /translation="MKIAIACDHGGYHLKEELLQFLQTAGHDVKDFGCYDANSIDYPD
                     FIFPAAEAVARGEFERGVVICTTGIGASICANKVKGIRCALCTDEYSAKMTRDHNDTN
                     VIAFGSNVTTPVRAMNMLDIWLHTDFSNGERHVRRIEKISAYEKTHK"
     gene            3955..4584
                     /gene="upp"
                     /locus_tag="PLR57_00085"
     CDS             3955..4584
                     /gene="upp"
                     /locus_tag="PLR57_00085"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQQ39881.1"
                     /translation="MSKIITIDHPLVQHKLSLLRDKDTGCKAFRELVYELAMLMAYEV
                     TRDLELKEIEIETPICKTKVQTLANEKIAVVPILRAGLGMADGIMSLIPNAKMGHIGL
                     YRNPDTLMPTGYYSKLPQDIAERETIIVDPMLATGNSGNEAIRILKNAGTQSIRFVSL
                     IAAPEGLEAMQKANPDVDIYVAHIDEKLNSHGYIVPGLGDAGDRLFGTK"
     gene            4825..5139
                     /locus_tag="PLR57_00090"
     CDS             4825..5139
                     /locus_tag="PLR57_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39882.1"
                     /translation="MRSIIEEIADAERRAEEIVKAAAVQNRELLAQTREQAEQALVLA
                     EAEEKSLTEKALSQASAQGEAQAAKTLAAMEQEADELCGKAKAKLDGAVHYLVDKVQS
                     RA"
     gene            5148..7088
                     /locus_tag="PLR57_00095"
     CDS             5148..7088
                     /locus_tag="PLR57_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HQQ39883.1"
                     /translation="MKRVTLVAHKADEANLLSALQATQSVEVIAEQTEAPDLTALERA
                     EEQVQSLKQALVTLRPFAEKRGFFSAPPEASAAEIGQSLPGGLAISKNLETISRELST
                     VRGEMEKNDTLISTLRPWEHFPADMQSFQNVRGIKYFTGVIASSDVEKLSAIDEAAEY
                     QLFNEGSMRTCVVACAQEEAKSIANFLKSLAWTDFAFPKMNGTPAEAMTELAERNRAL
                     AAQKFELESALSEEAKGASSTIESALDAAIIERDRTAAAAEIARSSATFQLEGWVPSD
                     KTEAVEKAVRSVTDAYYFTVRDAEEGEIPPSYVENNHFVTPFEQVTNLYSRPDPNGID
                     ASPYMAPFYVLLFGLMLSDTGYGIALSVLTAIFIKFKKPGGMSGGFARVLFWGGISTA
                     VWGVLVGTFFGMDFDTLLGTNNMFPLLVDPMSNPIGMLILCFGLGVLHILFGVALKMK
                     LSFSRGDWQTAIFDNLSWILIVCGLILYAVPSAVSAVPAVVGTVGLAAAGLGALMILL
                     FKGREKKNPFSRTISGLGELYQVTGYLSDILSYARLFALGIATGVIASVFNELCKMLM
                     QSPILILKILGIIVACGLLVALHGFNIAINTLGAFVHCARLQYVEFYGKFYETGGRAF
                     RPLGYQTKHVRVKDELSSSLSE"
     gene            7108..7590
                     /locus_tag="PLR57_00100"
     CDS             7108..7590
                     /locus_tag="PLR57_00100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HQQ39884.1"
                     /translation="MDIQWGVIFAAAGAAMAAVMAGVGSAMGVGLAGQAAAGVVSEDP
                     DRFGSCLLLQLLPGTQGIYGLLIAFVIATKAGLLSGGSDAVTLTNGLNLFLAAIPIAF
                     GGLLSAIAQGKVAVAGINMIAKKPEEQGKAMLMTVMVETYAVLALLISFLLVNGVKVG
                     "
     gene            7634..8218
                     /locus_tag="PLR57_00105"
     CDS             7634..8218
                     /locus_tag="PLR57_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HQQ39885.1"
                     /translation="MSTLSISGTNKLAERIIGDAEAEAQRTRAEANAAAGEIRAENER
                     AVKVLRASYSIKRDAAVASRKAGFLTRAALDGRKSALAKKRAIIDEAFQSAYEALLKL
                     DETQRARICEQLLRSEAEGGETVVPAKADRKGIERVLAGMKEKRLTLSTEDAPVDGGF
                     LLLSGSYEKDCSFRSLLNEARQNEETSIAKMLFD"
     gene            8235..>8535
                     /locus_tag="PLR57_00110"
     CDS             8235..>8535
                     /locus_tag="PLR57_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HQQ39886.1"
                     /translation="MPQPSYPYACARISALEKGLLNSQTIRRMAEGSLEDAMRALLDA
                     RYGNMPDATSEDCERMIENVRRQTTATILELSPDPALTDLFLLETDALNLKILIKA"
BASE COUNT         2053 a         2336 c         2512 g         1634 t
ORIGIN      
        1 cgcggcgctg gactcggtgt tcggcttcgc cccgctcggc acgcccgagc cggcgcgccg
       61 cggcggacgg cctgtcatgc tggtgggccc gcccgccgtc ggcaagaccg tgaccgtcgc
      121 caagctcgcc gccgcttccg acaagctctg ggcgagaatt atccgcgcgc cgggactggg
      181 actgcagtac ttgaccaccc gcgaacccga gcaggatatg atcgaagtcg ccatcgcggc
      241 gttttatctc gccatgaaca aaggcaagtc cgataaattg atgccggaaa acgatgcaga
      301 agccgaaccc gcgcagagcg aaacgaagga gcctgacaac acttgaccat gacggttgcc
      361 gaaaccgttc ggctggcaaa actgaggctg gaacccgttg ccggtgagga aagcctgcag
      421 caggcaaaac tgcttgttgc ggcggtcatt ggagcaaaag ccgatgcgct gatggtgcat
      481 acctggatgg acgtgaccga agagcagctt gtgctcattt gcgaccttct tgagcggcgc
      541 ttaaaaggcg aaccgctgca gtatatcctc ggcgagtggg agttcatggg cttgccgttt
      601 tacgtggatg agcgcgcttt aatcccgcgg caggataccg aactgctctg cgaaaaagcg
      661 ctggagatca tccgaaccca acacagcaaa agcgtactcg atctctgcac gggcagcggg
      721 tgccttgcgg tttcaattgc aaagcttgcg gaaacggatt taacggttac cgcttcggat
      781 gtcagcactg acgcactggc actggcaaag gaaaacgcaa tcctcaacgg cgtggacgcg
      841 agcatcggct ggatcgaaag cgatctgttt gataagatac cgggaacgtt tgacctcatc
      901 gtctgcaacc cgccgtacct gacgagaccg gatatggagc atctgcagac ggaagttgcg
      961 tttgagccgc gcctcgcgct gtttggcggg gaggacgggc tggatattta ccgccggatt
     1021 gccagcgagt acaaggctca cctgaatccc ggcggaacgc tgctgctgga aattggcagc
     1081 acgcaggctg aaagcgtttc cgcgctgttc gggcaaactg caacagtatt taacgattta
     1141 tgcgggaacc cgcgcgtaat acaagttcaa caaagcgagt aagagaaact atgctggaaa
     1201 aactcattgg aattaaagaa cgttacgatc ttctctctgc gaagctggcc gagcccgacg
     1261 ccatggcgga taaagacgca tggcgagcga tggtcaagga gcgcgcttcg ctggaagaga
     1321 tcgtgcttgc gtttgaagaa tatcaaactg cgcaggagtc gcttgaaaac tgccgcgcca
     1381 tgctaaacga ccacctcgac gcggaaatga aggaactcgt ccacgcagaa attgcggaac
     1441 tggaagagaa agccgtttcg gttgaggagc gcctgcgtgt attgctgctg cccaaagacc
     1501 cgaacgacga taaggacgtc attctcgaaa ttcgcgcggg aacgggcgga gacgaagcct
     1561 cgctcttcgg cgcggatttg ctgcggatgt atcttcgcta cgctgaacga aattcctata
     1621 aagtggaata tctttcgagc aacatgaccg atatgggcgg cgtcaaggaa gttgtgctct
     1681 ccatcagcgg caagcgcggc gcgtacagca ggctcaaata cgaaagcggt gtccaccgcg
     1741 ttcagcgcgt accggaaacg gagtcgcagg ggcgcatcca taccagcgct gcaacggtcg
     1801 ccgttctgcc ggaagtggac gatattcaaa tcgacatcaa ggaaagcgac cttcgcatcg
     1861 acacctaccg ttccagcggc gcgggcggcc agcacgtcaa taagacggag tccgccatcc
     1921 gcattacgca cctgccgacc ggcatggtcg tttcctgtca ggatgaaaaa tcgcagctga
     1981 aaaacaagga gcaggccatg cgcgtgctca agagcagact gttcgacttt taccagggcc
     2041 agcaggatgc ggagcgcgcg ggcgcgcgca agagccagat cggctccggc gaccgcagcg
     2101 aacgaatccg cacctataat ttcccgcagg ggcgcgtaac cgatcaccgc atcggcgtaa
     2161 cgctctaccg tttggaagcg tttctcgacg gggatatgga cgaactcatc gacgcgctga
     2221 ttcttgccga acgcagccaa aagctctccg gcggaacgga ctgattcagc atgcgcggcg
     2281 aacaggaaca gcccttgtac caaacgcagg tcatcaacgt ggagcgccag cccgaagcgg
     2341 gttcggttca ggcggccaag ctgattcagg cgggcgagct ggttgcgttt ccaacggaaa
     2401 cggtgtacgg cctcggcgcg gacggcttga acggagatgc ggtacaaaag atttttgaag
     2461 caaaggggag acccgcggat aatcctttaa tcgagcatgt tgcaaagaga agcgacgtgc
     2521 gcgagctctg gctgaagata cccagagttg cggatacgct catggacgcg ttctggccgg
     2581 gcccgctgac gctggttgcg caaaagagcg accacgtacc aagtgaagta acggcggggc
     2641 tgaacacggt cgcggtgcgc atgccgctca acaagaccgc gcgctcgctc atcagtaagg
     2701 cgggcgtacc cattgcggct cccagcgcga accgctccgg caggccgagc ccgaccaccg
     2761 cgcagcacgt gctggaagat atggacggca ggattccgct gattctggat ggcggcccct
     2821 gcaagtacgg cgtggagtcc accgtgctac tgctggaagg cgaacccacg attctccgtc
     2881 ccggcgcggt caccaaagag atgctcgaag cggtcatagg cgaagtgcag gtggcaaaaa
     2941 gcatcctcca gcccatgaag cagggcgaag tcgccgcgtc tccgggtatg aagtacaagc
     3001 actacgcgcc gaatgcggaa gtcgtagtag tcaccggcga accggaacgc gttgcaagaa
     3061 cgataaacgc gctctaccgg caagcggaag cggaaggcat tgactgcgaa attgcggcca
     3121 cggagcagac gaagaacttc taccgcggta agaaatatgt tatactcggc gatagaaaat
     3181 cgccggaaac gctctgcgca aacctctttt ccgcgctgcg cgagatggat caaagcgccg
     3241 accgcatctt tgccgaaggc gtaccggcgg aaaatgaagg cctggcctat atgaaccgcc
     3301 tgctcaggag cgccggcttc aaggtaatcg aagccgagta agagtacaac agaaaagcga
     3361 aacgaaacac gaccgaagcg ggtcaggaac ggaaaggatg aacagtatga aaattgcaat
     3421 cgcatgcgac cacggcggat atcatctcaa ggaggaactg cttcagtttc tgcagaccgc
     3481 gggacacgac gtcaaagact ttggctgcta cgacgcaaac agcatcgatt atccggactt
     3541 catctttccc gcggcggaag cggtagcaag aggcgagttt gagcgtggcg tcgtcatctg
     3601 cacaactggc atcggcgcca gtatctgcgc aaacaaagta aagggcattc gctgcgcgct
     3661 ctgcacggac gagtattcgg caaagatgac gcgcgaccat aacgacacca acgtcattgc
     3721 gttcggctcg aacgtgacaa cccctgttcg cgccatgaac atgctcgata tctggctgca
     3781 caccgatttt tcaaacggag aacggcatgt gcgccgcatc gaaaaaatct cagcctacga
     3841 aaaaacgcac aaataggcga gcaagctccg cgcagccgag cgcgggcaaa atacgccgaa
     3901 caagcgaatc aattaaatat caattttgaa taatatgcac taaggaggct cgatatgtcc
     3961 aaaattatca ccatcgatca cccgctcgta caacacaagc tctcactgct gcgggacaag
     4021 gataccggct gcaaggcgtt ccgcgaactg gtttacgaac ttgccatgct catggcgtac
     4081 gaagtgacac gggatctgga actcaaggaa attgaaattg aaacccccat ctgcaaaacg
     4141 aaggttcaga cgctcgccaa tgaaaaaatc gcagttgttc cgattcttcg cgcaggcctc
     4201 ggcatggcgg acggcattat gagcctgatt ccgaacgcaa agatggggca tatcggcctc
     4261 taccgcaacc ccgacacgct gatgcctacc ggctactaca gcaagcttcc gcaggacatt
     4321 gcggagcgcg aaaccattat tgtcgatccg atgctggcaa ccggaaactc cggcaacgaa
     4381 gccattcgca ttctcaagaa tgccggaacg cagagcatcc gttttgtgag cctcatcgcc
     4441 gctccggaag ggctggaagc catgcagaag gcgaatccgg acgtggacat ctatgttgcc
     4501 cacatcgacg aaaaactcaa cagccacggc tatatcgtac ccggtcttgg cgacgctggc
     4561 gacagactgt tcggaacaaa atagtttttt acgaaatcag gcaggaatca atagcggccg
     4621 actgaaggaa tgcgaaaaaa ttacttctga tttcggtcgc tatttttctg ttctatttca
     4681 tgatttctat gttaatatat atgaggctcg tttaaaacgc cgtttcagga cgtttaaccg
     4741 cagccgaagc aactgcaact aaaatccttt taattcttat ataagaatga tgcagtacag
     4801 acaaccagga tacggaggtt actcatgcgg tcgatcatcg aagaaatagc cgatgcggaa
     4861 cgacgcgcgg aagaaatagt gaaagccgca gcggtgcaga accgcgagct tcttgcgcaa
     4921 acacgcgaac aggccgaaca ggctcttgtt ttagcggaag cggaagaaaa gagcctgacg
     4981 gagaaagcgc ttagtcaagc aagtgcgcag ggcgaggcac aagccgcaaa aacgctcgcc
     5041 gccatggagc aggaagccga cgaactttgc ggaaaagcga aagcaaagct ggacggagcg
     5101 gttcattatt tagtggataa ggtgcaaagc agagcatgat tgttcagatg aaacgagtta
     5161 cgctcgttgc gcacaaggcg gatgaagcaa atctgctgag cgccctgcag gcgacgcagt
     5221 cggttgaagt gattgccgaa caaacggaag cgcccgacct gaccgcgctc gaacgagcgg
     5281 aagaacaagt gcaaagcctc aagcaggcgc tcgtgaccct gcgcccgttt gctgaaaagc
     5341 gcgggttctt cagcgcgccg ccggaagcaa gcgctgcgga aatcgggcag tctctgccgg
     5401 gcgggctggc catcagcaag aacctggaaa cgatctccag agagctttcc acggttcgcg
     5461 gcgagatgga aaagaacgat acgctcatca gcacgctgcg tccctgggag cattttcccg
     5521 cggacatgca gtcgtttcag aacgtacgcg gcatcaaata tttcacgggc gttatcgctt
     5581 cctcggacgt tgaaaaactc agcgccatcg acgaagcggc ggaatatcag ctcttcaacg
     5641 aaggcagcat gcgcacctgc gtcgttgcct gcgcgcagga agaagcaaaa tccatagcaa
     5701 acttcctcaa gtcacttgcg tggacggatt ttgcgttccc caagatgaac ggcacacctg
     5761 cggaagcgat gacggaactc gcggagcgca accgcgctct tgcggcgcaa aagttcgaac
     5821 tggagtccgc gctttcggaa gaagcgaaag gcgcttcgtc taccattgag agcgcgctag
     5881 acgccgcgat catcgagcgc gaccgcacgg cggccgccgc cgagattgcg cgttccagcg
     5941 ccaccttcca actggaaggc tgggtgccct ccgataaaac ggaagcggta gagaaagctg
     6001 ttcgctcggt gaccgacgcg tattacttta ccgtgcggga tgccgaagaa ggtgaaattc
     6061 cgccttccta tgtagagaac aaccacttcg ttaccccatt tgagcaggta acgaatctgt
     6121 attcgcggcc cgatcccaac ggaattgacg cttctcccta tatggcgccg ttctatgtgc
     6181 tcctgttcgg gcttatgctg agcgataccg ggtacggcat tgcgctttcc gtgctgacgg
     6241 caattttcat taagtttaag aaaccgggcg gcatgtctgg cggttttgcg cgcgtgctgt
     6301 tctggggcgg catctccacg gcggtatggg gcgtactggt cggcacgttc ttcggtatgg
     6361 atttcgacac gctgctggga accaacaaca tgttcccgct gctggtagac cccatgtcga
     6421 atccaatcgg catgctgatt ctctgcttcg ggctgggagt actgcatatt ctcttcggcg
     6481 tagcgctcaa gatgaaactc tctttttcca gaggcgactg gcagacggct atcttcgata
     6541 acctgtcctg gattctgatc gtttgcggct tgattctgta tgccgttccg tcggcggttt
     6601 ctgccgttcc cgcggtggtt ggcacggttg gcttggcggc ggcggggctg ggcgcgctga
     6661 tgatactgct cttcaaaggg cgcgagaaga aaaacccgtt ttccagaacg atctccggtc
     6721 tgggagagct ctatcaagta accggatatc tttcggacat cttgtcctac gcgcgtctgt
     6781 tcgcgctggg cattgcaacg ggcgttatcg cttccgtgtt caacgagctc tgcaaaatgc
     6841 tcatgcagtc gccgattctg attctgaaga ttctcggcat cattgttgcc tgcggcctgc
     6901 tcgttgcgct gcacgggttc aacattgcaa tcaacacgct gggcgcgttc gtgcactgcg
     6961 cgaggctcca gtacgttgag ttttacggaa aattctatga gacgggcggc agagccttcc
     7021 gtcccttggg atatcaaaca aaacatgttc gagtgaagga tgagcttagt tcgtccttga
     7081 gcgaataaca aaggaggatt tttcactatg gatattcagt ggggtgtcat ttttgcagcg
     7141 gcgggtgccg cgatggccgc cgttatggcg ggtgttggaa gcgcgatggg cgttggcctt
     7201 gccggtcagg cagcggcggg cgtcgtttcg gaagacccgg atcgtttcgg ttcgtgcctc
     7261 cttctgcagc tgcttcccgg tacgcagggt atttacggcc tgctgatcgc tttcgttatc
     7321 gcaaccaaag cgggcctgct ctccggcgga tccgacgcgg ttaccttgac gaacggcctg
     7381 aatctctttc ttgccgcaat tccgatcgcg ttcggcggat tgctctccgc gattgcgcag
     7441 ggcaaagttg ccgttgccgg catcaacatg attgccaaga aaccggaaga acagggcaaa
     7501 gcgatgctca tgaccgtcat ggtggaaacc tacgcggtgc ttgcgctgct catctccttc
     7561 ctgctcgtca acggcgtaaa ggttggttaa taaagcgcaa agcaatcatt ttgtaccgat
     7621 gaaggagcaa ccgatgtcta ccttatccat atccggaacg aacaagctcg ccgagcgcat
     7681 cattggcgac gccgaggcgg aagcgcagag aacccgtgcg gaagcaaacg ccgccgcggg
     7741 tgaaatccgc gcggagaacg aacgcgccgt caaagtgctg cgcgcttcct attcgatcaa
     7801 gcgcgatgcg gcggtggcga gccggaaagc cggttttctg acccgcgccg cgctggacgg
     7861 aagaaagagc gcgttggcga aaaagcgcgc catcatcgac gaggcgtttc agagtgctta
     7921 tgaagcgctg ctcaaactgg acgaaacgca gcgcgcgcgc atttgcgagc agctcctgcg
     7981 ctcggaagcg gaaggcggcg aaacggtcgt tccggcaaaa gccgaccgca aggggattga
     8041 gcgcgttctt gcaggaatga aagaaaagcg cctgacgctt tcaacagagg acgcgcccgt
     8101 tgacggcggt tttctgctgt tgagcggaag ctacgaaaag gactgctcgt tccgttcgct
     8161 cctgaacgaa gcgcgccaaa acgaagaaac cagcatcgca aagatgctgt tcgactgacc
     8221 ggaggggagg ttctatgccg cagccaagtt acccctacgc gtgcgcgcgc atcagcgcgc
     8281 tcgaaaaggg actgttgaat agccagacca tccggcgcat ggcagaaggg tcgctggagg
     8341 acgcaatgcg cgcgctcctc gacgcgcgat atggtaacat gccggacgcg acgagcgagg
     8401 actgcgagcg catgattgaa aacgtccgcc gccagacgac cgcgaccatt ctggagctct
     8461 cgcccgatcc cgccttgaca gacctgtttc tgctggaaac cgacgcgctg aacctgaaga
     8521 ttctcattaa agcgc
//
LOCUS       DAOMGY010000003         7844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_9337_length_7844_cov_3.150854, whole genome shotgun sequence.
ACCESSION   DAOMGY010000003 DAOMGY010000000
VERSION     DAOMGY010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9337_length_7844_cov_3.150854"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLR57_00115"
     CDS             complement(<1..658)
                     /locus_tag="PLR57_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ39887.1"
                     /translation="MLLIGNGRLVTRDNGLFIENGAVVTDSNLILDVGETAALRARYP
                     QAEFIDANGGLIMPGLINTHNHIYSAFARGLSIKDYSPRDFGDILEGMWWKIDRLLTK
                     ENDSLSAYAVYIDCIKNGVTTVFDHHASYFDIPGSLDTIADAAKELGVRTSLCYEVSD
                     RDGEVKMKEAVLENARMIRRAEGDSSDMLKGMMGLHAAFTLSDETLSLCKSHTPETTG
                     F"
     gene            965..2200
                     /gene="thrC"
                     /locus_tag="PLR57_00120"
     CDS             965..2200
                     /gene="thrC"
                     /locus_tag="PLR57_00120"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQQ39888.1"
                     /translation="MKRIAGYRCTICGREYPYGPELMTCPSCGEKGILDIVFDYGEIK
                     SVFSKETLKKDSNNSMWRYRALMPLVGEGLADFLRIGWTPLYRSLRLGKQIGLDTLYI
                     KDDGLNPTGSLKDRASGVAVAKAIELGYDTIACSSTGNAASSCAGNAARMGLKTVIFV
                     PERAPMGKVAQLLIFGANVISVKGDYRQTFELSKAAIEKWGFYNRNAGINPILTEGKK
                     TVALEIAEQLNWNPTDWVAVSVGDGCTIGGVYNGFYDLYQLGMIDRIPKILGVQSTGC
                     CPFVDADEHGGELVPTPENTLADSIAVGVPRNPVKAQRAVKASGGKWIAVSDDEILEA
                     MRLLGRSEGVFGEPAGVTATAGVVKAVASGVIRSNESVTVISTGSGLKDVKNAMLAAG
                     EPFKCEPDIKALEASGRIH"
     gene            2586..3854
                     /locus_tag="PLR57_00125"
     CDS             2586..3854
                     /locus_tag="PLR57_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQQ39889.1"
                     /translation="MKKIIALLAVVMMVFSLAACQTTTEPAAEVTATPETVEAAEEPA
                     AEAPAEEPALKIGVILVHDDNTGYDAAHIEGIKTAAANVGIPDTSIIWKYNIGEDETC
                     YDTAIDLVEQGCTYIFSDSYGHQTHMQQAASENPDVTFVSMTGDGAALSGLPNFKNAF
                     THTFESRYVSGVVAGMKLKEMLDAGVVTDPYVGYVGAYPYAEVVSGFTGFYLGIKSIV
                     PDAHMDVTYTNSWYDPTAEAEAANALISKGCCIISQHADSTGAPSAVQAALDAGTPVY
                     CVGYNIDMLAVAPTAALTSAQNNWSAYYTYAFNCALTGEDIATDWSEGYATGANMIST
                     LGPSCAAGTQEKVDEVIAGIIDGTVNVFDTSTFTVGGAVLDPGTFFIDTDGDWVPDSG
                     TAIENGIFYESQLRSAPYFGIIIDGITTLN"
     gene            4108..5637
                     /locus_tag="PLR57_00130"
     CDS             4108..5637
                     /locus_tag="PLR57_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ39890.1"
                     /translation="MVNVTKTFGEVVANDKVCLDIKRGEILSLLGENGSGKTTLMNML
                     AGIYFPDSGEIYINGKAVTIASPKDAFDLHIGMIHQHFKLVDVLTAAENIILGLNEST
                     VVDLKKVRAAVKQICDRYGFVVNPDKKVYDMSVSEKQTLEIVKVLYRGADILILDEPT
                     AVLTPQETQKLFCVMRNMKEDGKAIIIITHKLHEVLDVSDRVAVLRKGHYIGDVPTCE
                     TDAMKLTEMMVGRAVSLDIARSTPVNPQPRLVVKNLTVCDSDGVTRLSGVDFTAYSGE
                     ILGVAGISGSGQKELLEAIAGLQYCQTCSSMQFYPEDSDKPKELSGMHPIDIKRLGVA
                     LAFVPEDRLGMGLVGSMNLTDNMMLRSYSDGSGPFADRKKPRKLAETVVRDLEVVTPN
                     LNTPVRRLSGGNVQKVLVGREIAMQPQLLMSAYAVRGLDINTSYNIYHLLSEQKEKGA
                     AVIFVGEDLDVLLELCDRIMVLCSGHVNDIVDARTTTKDEVGYLMTYLKEGRESHEQK
                     A"
     gene            5621..6736
                     /locus_tag="PLR57_00135"
     CDS             5621..6736
                     /locus_tag="PLR57_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ39891.1"
                     /translation="MSKKREQKSREPFIRMSKRDHMPLWKSFGVRLIGLALALVICAI
                     IIYSLTKLNPLKVYGAMFKGAFGTEKRIWVTIRDIMMLLCIAVGLAPAFKMRFWNIGA
                     EGQVLIGGIAAAACMIYLGKSLPTMALIVIMFFGSAVAGGVWGTIPAVFKARWNANET
                     LFTLMMNYIAIQLTSFFVAKWEHPFGSNTVGIINQMTKAGWFPTVFGQMYTLNIMIVL
                     TLTVLMYLYLRYSKQGYEIAVVGESENTARYAAINVKKVIIRTIALSGAICGVAGFVA
                     VAGASHTISTNTAGGRGFTAIIVAWLSKFNTFVMIGIAALLVFLDKGAIEIASQFDLN
                     DFVAEMITGIILFFVLGSEFFINYKIRFRGGRKEAGK"
     gene            6733..7719
                     /locus_tag="PLR57_00140"
     CDS             6733..7719
                     /locus_tag="PLR57_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ39892.1"
                     /translation="MTQLISLIQAAVVFGTVILFGAVGEILAEKSGNLNLGVPGIMYL
                     GGIAGLATAFFYENLAVNPSAGIAVILTLAASFIAALLGGLIYAVLTITLRANQNVTG
                     LTLTIFGGGIANLFGGTLNKMQGGAGQISVALASGAFRTNIPFLSDHLGAVGRIFFSY
                     GFLVYLAIALAFLVSWVLNKTSVGLNLRAVGENPATADAAGISVTKYKYLATCIGAGI
                     SGLGGLYYTIDYIKGTWSTDGSIEKLGWLAVALVIFATWKPKRSIWGAYLFGALFWLF
                     FYIPGLTRSSQEIFKMLPYLVTVLVLVISALRKKREDQPPASLGNAYFREER"
BASE COUNT         1840 a         2083 c         2110 g         1811 t
ORIGIN      
        1 ggaatcccgt cgtttccggc gtgtgcgact tgcagagcga gagcgtttcg tccgagagcg
       61 taaacgccgc gtgcagaccc atcatgccct tgagcatgtc gcttgaatcg ccttccgcgc
      121 ggcgaatcat gcgggcgttt tctagcacgg cttccttcat cttgacttcg ccgtcacggt
      181 cggaaacctc gtagcaaagg ctggtgcgaa cgccgagctc ttttgccgcg tccgcaatgg
      241 tgtcgagact gccgggaata tcaaaatagc tggcgtgatg gtcgaaaacc gtggtcacgc
      301 cgtttttgat gcagtcaata tagaccgcgt atgccgatag gctgtcgttt tccttcgtga
      361 gcaggcggtc gatcttccac cacatgcctt cgaggatatc accaaaatcg cgcgggctgt
      421 aatcttttat agagagtccg cgcgcaaacg cactgtaaat atggttgtgc gtattgatta
      481 accccggcat gatcagcccg ccgtttgcgt cgataaactc cgcctgcggg tagcgcgcgc
      541 gcagggctgc ggtttcgcca acgtcaagaa taaggttgct atccgtaaca accgcgccgt
      601 tttcaataaa caggccgtta tctctggtaa cgagcctccc gtttccaatc agaagcatat
      661 gaatttcgtt ctcccttcgt gatctggtgt cgtaaagacg agaaaagact tttttctaga
      721 caggttctat aaaaccgaca tcatacaact cagttacaat cgtattatac tatacgtttt
      781 ctcgggcacg caatacgagc gggggcttta ggcacaaaat atttttgggc ataataattt
      841 tgtatagcgc tttattttac tgtacatttt aaaatttgtg cggtatagtg tagtatatct
      901 tcttgattca cgcaaacctt tatttaaaaa gcaaataact gatcgttcat tacaggagta
      961 acacatgaag cgtattgctg gttatcgttg tacgatttgt ggcagggaat acccatacgg
     1021 gcctgagctg atgacttgtc cgtcctgcgg cgaaaagggt attctggata tcgtttttga
     1081 ctacggcgaa ataaagtccg tcttttccaa agaaacactg aaaaaagatt ccaacaacag
     1141 catgtggcgt tatcgggcgc ttatgccgct ggttggcgaa ggccttgccg atttcctcag
     1201 gatcggctgg acgccgctct accgttcgct tcgtctgggc aagcagatcg gtttagacac
     1261 gctctatatc aaggacgacg gactcaaccc gaccggctcg ctcaaggatc gcgccagcgg
     1321 agtcgcggta gcgaaggcca tcgagttagg ctacgatact attgcatgtt cttccacggg
     1381 caacgccgcg agttcctgcg cgggcaacgc cgcgcggatg gggctgaaga ccgttatctt
     1441 cgttccggaa cgcgcgccca tgggcaaggt cgctcagctt ttgatttttg gcgcaaacgt
     1501 catctcggtc aaaggcgatt accgccagac gttcgaactg agcaaagcag ccatcgaaaa
     1561 gtgggggttc tataaccgca acgcaggtat caacccgatt cttacggaag gcaaaaagac
     1621 cgtagctctt gaaattgccg aacagctcaa ctggaacccg acggattggg ttgccgtttc
     1681 ggttggagac ggctgtacca ttggcggcgt atacaacgga ttctacgatc tctatcagct
     1741 cggtatgatc gatcggatcc cgaaaatcct tggtgtgcag tccacgggct gctgcccgtt
     1801 cgtggacgcg gacgagcacg ggggtgagct ggtgcccaca ccggagaaca cactcgcgga
     1861 cagcattgcg gtgggagtgc cgcgcaatcc cgtcaaggcg cagcgcgcgg taaaggcaag
     1921 cggcggaaag tggattgccg tctcggacga cgagattctg gaagccatgc gcctgctcgg
     1981 ccgcagtgaa ggcgtgttcg gtgaaccggc gggcgttacc gctacggctg gcgttgtcaa
     2041 ggcggttgcc agcggtgtga ttcgctccaa tgagagcgta acggttattt caacaggcag
     2101 cggattgaag gacgttaaaa acgcaatgct tgcggcgggc gaaccgttta aatgcgagcc
     2161 ggacatcaaa gcgctggagg ccagcggaag aatacactga tcattacggg cgaacagaaa
     2221 acagtgcata tttataagga ttagatgcaa taatatcccg cgaatgcttg cttgaataaa
     2281 ataaacatgc taaaatgttt cagaaaaatg ttcccgcaag cactgcaata ggcgtattct
     2341 gcaatatatt gcgcattatc gaagaattgc agcgcgacac gaactgcata aatatgtatt
     2401 attcttgcag aattttacaa agccgcttgt tttgagacgt agataatggt acaataagga
     2461 acgttttaga ggaagcaata acaagagaat caatggcgtc tattttcctc gtattttttt
     2521 gtaaacaagg ttgttccacc caataaaggt gctgcaataa atcaaaaata agcaggagga
     2581 aaaccatgaa aaagatcatt gctctgttgg cagtcgtcat gatggtgttc tctctggcag
     2641 cctgccagac caccaccgaa cctgcggctg aagtgaccgc aacgcccgag accgtagaag
     2701 cggccgaaga acccgccgcc gaagcgcccg ccgaagaacc cgccctcaaa atcggcgtaa
     2761 tcctcgttca cgacgataac acgggctatg acgccgcgca catcgaaggc atcaagaccg
     2821 ctgccgcgaa cgttggtatt ccggacacca gcatcatctg gaagtacaac attggcgaag
     2881 atgaaacctg ctacgatacc gcaatcgacc tcgttgagca gggctgcacc tacatcttct
     2941 ccgacagcta cggccatcag acccacatgc agcaggccgc ttccgaaaat ccggacgtga
     3001 ccttcgtctc catgactggt gacggcgctg ccctgagcgg actgcccaac ttcaagaacg
     3061 cgttcaccca cacgtttgag agccgttatg tctccggcgt cgttgccggc atgaagctca
     3121 aggaaatgct ggatgcaggc gttgtgaccg atccttatgt cggttatgtc ggcgcttatc
     3181 cgtatgccga agtggtcagc ggcttcaccg gcttctatct tggcatcaag agcatcgttc
     3241 ccgatgcgca catggacgtg acctatacca actcttggta tgatccgacc gctgaagcgg
     3301 aagctgcgaa cgccctgatt tccaagggct gctgcatcat cagccagcac gctgactcca
     3361 ccggtgctcc gtccgccgtc caggccgctc tggatgcggg tacgcctgtg tactgcgttg
     3421 gctacaacat cgacatgctg gccgtcgctc cgaccgccgc tctgacctcc gcgcagaaca
     3481 actggagtgc ttattacacc tatgcgttca actgcgctct gacgggcgaa gacatcgcga
     3541 ccgactggtc cgaaggctat gccaccggcg ccaacatgat ctccacgctc gggccgagct
     3601 gtgcagccgg cacgcaggag aaggttgacg aagtcatcgc gggcatcatc gacggaaccg
     3661 tcaacgtgtt cgacacctcc accttcaccg ttggtggcgc ggtgctcgat cccggcacgt
     3721 tcttcatcga taccgacggc gattgggttc cggattccgg taccgccatc gaaaacggca
     3781 tcttctacga gagccagctc cgcagcgcgc cgtactttgg catcatcatc gacggcatca
     3841 cgacgctcaa ctaacttgta actgttcaat gacatgaaag aaccggggac gggaaaccgc
     3901 tcccggttct tcgtcttttg acaaacgaat acagaaaagt ctatcgcgac gcggtcaatt
     3961 cacagttcgt ttgccgttcg gtacgattct tcccgtttac tccatgtttt tgtttacacc
     4021 ataagaacat ctatgctaaa atattttaag caagtctgaa tctaagatag aggggtcaag
     4081 cgtgtggaga attcagtagc gatccgaatg gtcaacgtta cgaagacctt cggcgaagtg
     4141 gtggccaacg acaaggtctg cctcgatatc aagcggggcg agatactgtc cctgctgggc
     4201 gaaaacggca gcggcaagac cacgcttatg aacatgctgg cgggtatcta tttcccggac
     4261 agcggcgaga tctatatcaa cggcaaagca gtgaccatcg cctcaccaaa ggacgcgttc
     4321 gatctgcata tcggaatgat tcatcagcac ttcaaactcg tagacgtctt aacagcggca
     4381 gaaaacatca tactcggcct gaatgaatcg accgtcgtcg atttaaaaaa ggtgcgcgcc
     4441 gccgtcaagc aaatctgcga ccggtatggc tttgtagtca atcccgataa aaaagtatac
     4501 gacatgtccg tctccgaaaa gcagacgctg gaaatcgtca aggtgctgta tcgcggcgcg
     4561 gacattctga ttctcgatga accgactgcc gttttaacgc cgcaggaaac gcaaaagctc
     4621 ttctgcgtca tgcggaacat gaaggaagac ggcaaggcga tcatcattat tacgcataaa
     4681 ctgcacgagg ttttggatgt ttccgaccgc gttgccgtgc tgcgcaaagg ccactatatc
     4741 ggcgatgttc cgacctgcga aaccgacgct atgaaactga ccgaaatgat ggtcggccgt
     4801 gcggtttcgt tggatatcgc gcgctccacg cctgtcaacc cgcagccccg gctggtcgtt
     4861 aagaacctga ctgtctgcga ttccgacggg gtcacacggc tcagcggcgt ggactttacc
     4921 gcgtacagcg gcgaaattct cggtgttgca ggtatttccg gcagcggaca aaaggaactg
     4981 ctggaagcca tcgcagggct ccagtactgc cagacctgca gcagcatgca gttttatccg
     5041 gaggacagcg ataagcccaa ggagctctcc ggcatgcatc caatcgatat taagcgtttg
     5101 ggcgtagctc tcgctttcgt gccggaagac cgccttggca tggggcttgt cggcagcatg
     5161 aacctgactg acaacatgat gctcagaagc tattcggacg gctcaggccc gtttgcggat
     5221 cgcaagaagc cccgcaagct cgcggaaacc gttgtgcgcg atctggaagt cgtaaccccg
     5281 aacctcaaca cgccggtgcg cagactctcc ggcggaaacg tgcagaaggt tctggtcggc
     5341 agagagattg ccatgcagcc gcagctgctc atgtccgcct atgctgtgcg cggactggat
     5401 atcaacactt cctataatat ttaccacctg ctcagcgagc agaaagagaa gggcgccgcc
     5461 gtcatctttg taggcgagga tctggacgtg ctgctggagc tctgcgaccg gatcatggtg
     5521 ctctgctcgg gacatgtaaa cgacattgtc gatgcgcgca cgaccacaaa agatgaagtc
     5581 ggttatctga tgacttactt gaaggaaggg agggagagcc atgagcaaaa agcgtgagca
     5641 aaaatcccgc gaaccgttca tccgtatgtc caagagggat catatgcccc tctggaagtc
     5701 ttttggcgtt cgtttgatcg gtttggcgct ggcactcgtt atttgcgcca tcatcatcta
     5761 ctccctgacg aaactcaacc cgctgaaagt atacggagcc atgttcaagg gcgcgttcgg
     5821 tacggaaaaa cgcatctggg ttaccattcg cgatatcatg atgctgctct gcatcgccgt
     5881 cggtttggcg cccgcgttca aaatgcgatt ttggaacatc ggcgcggaag gccaggtgct
     5941 catcggcggt atcgccgcgg ctgcctgtat gatttacctc ggtaagtcgc ttcccaccat
     6001 ggcgctcatc gttatcatgt ttttcggaag cgcagttgcc ggcggcgttt ggggaaccat
     6061 ccccgcggta ttcaaggcgc gctggaacgc aaacgaaacg ctctttacgc tgatgatgaa
     6121 ctacatcgcc attcagctga catcgttttt cgttgcaaag tgggaacatc cgtttggttc
     6181 caatacggtt ggcattatca accagatgac gaaagcgggc tggttcccga ctgtcttcgg
     6241 gcagatgtat acgctcaata tcatgatcgt gttaacgctg accgtactga tgtatctgta
     6301 tcttcgctat tccaagcagg gctatgaaat cgccgtcgtt ggcgaaagcg aaaacacggc
     6361 gcggtacgcc gcgatcaatg tgaaaaaagt catcatccgc accatagcgc tttccggcgc
     6421 aatctgcggc gtagcgggat ttgttgcagt cgcgggcgca agccacacga tttctaccaa
     6481 caccgccggc ggaagaggat ttaccgcgat tatcgttgcc tggctttcca agttcaatac
     6541 gttcgttatg atcggtattg cggcgctgct tgttttcctt gacaagggcg caatcgaaat
     6601 tgccagccag ttcgacctga acgacttcgt ggccgaaatg attacgggta tcatcctgtt
     6661 cttcgtgctc ggcagcgagt tctttatcaa ctacaaaatc cgtttccgcg gtggaagaaa
     6721 ggaggccggc aaatgacgca gctcatttct ttgattcagg cggcggtcgt tttcggcacg
     6781 gttattctgt tcggagccgt cggcgagatc cttgcagaga aatctggcaa cctcaacctt
     6841 ggcgtcccgg gcattatgta tctgggcggc attgcggggc ttgccaccgc attcttctat
     6901 gagaaccttg cggtaaatcc ctctgccggt atagcggtca ttcttacgct tgccgcatcg
     6961 ttcattgcgg cgctccttgg cgggctgatc tacgcggtgc tcacgattac gctgcgtgcc
     7021 aaccaaaacg ttaccggctt aacgctcacc atctttggcg gcggcattgc gaatcttttc
     7081 ggcggaacgc tcaacaaaat gcagggcggc gcgggacaga tttccgttgc gctcgccagc
     7141 ggcgcgttcc ggacgaacat cccgttcctt tcggaccatc taggcgcggt ggggagaatc
     7201 ttcttctcct acggtttcct tgtgtatttg gcaattgcgc tggcgtttct cgtcagctgg
     7261 gtgctgaaca aaacgagcgt tggcctgaat cttcgcgctg tcggtgaaaa cccggcaaca
     7321 gcggacgcgg caggcatcag cgttaccaag tataaatacc ttgcaacctg catcggcgcc
     7381 ggcatctccg gccttggcgg actgtattat acgatcgact atatcaaggg cacatggtcg
     7441 acggacggca gcattgaaaa gctcggatgg ctagcggtag cgctggttat attcgccacg
     7501 tggaagccta agcgcagtat ttggggcgca tacctatttg gcgcgctgtt ctggctgttc
     7561 ttctacattc cgggactgac cagaagctcg caggagatct ttaaaatgct tccgtacctt
     7621 gttacggtgc tcgttctggt catttccgca ctgcgcaaaa aacgcgaaga ccaacctccg
     7681 gccagtctgg gcaacgccta cttccgcgaa gagcggtagt atttgttcaa aaaaacctgc
     7741 tcaaggcttg cgcagtcaac tgcgcaagcc tttgtctttc agtttgtaag cgaaagtaag
     7801 gataaaatat atttagcaaa acagaaatat attatgaaat ttcg
//
LOCUS       DAOMGY010000004         7518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_9839_length_7518_cov_3.415383, whole genome shotgun sequence.
ACCESSION   DAOMGY010000004 DAOMGY010000000
VERSION     DAOMGY010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9839_length_7518_cov_3.415383"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..985)
                     /locus_tag="PLR57_00145"
     CDS             complement(185..985)
                     /locus_tag="PLR57_00145"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HQQ39893.1"
                     /translation="MEQNTAKPVNPPSKTRKIVSIVSIVTFVLLLVFLTVFVGKPLIS
                     TLKDPSAFRDWIDARGVWGKLIFIGMCILQVVVAFIPGEPFELAAGYAFGALEGTLLV
                     WVGLILGSTIVFLFVRKFGVKLVEAVFPLEKIDSLPILNNEKALNATAFVLFFIPGTL
                     KDLLTYAAGLTKIRLLPWVLITSVARLPSIITSTVSGSALGTQRYVLAAVVFGATALL
                     SGAGFLIYRATEKKRAMREAGDRIAAQTLYVPPEEDAAEQQADTNKPE"
     gene            1176..2158
                     /locus_tag="PLR57_00150"
                     /pseudo
     CDS             1176..2158
                     /locus_tag="PLR57_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
     gene            2544..3722
                     /locus_tag="PLR57_00155"
     CDS             2544..3722
                     /locus_tag="PLR57_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ39894.1"
                     /translation="MATILLIIIYLAFISLGLPDSLLGAAWPVAYAQLGAPVSYAGLV
                     SIFITVGTVISSLVSVRVIRKFGTGAVTAFCVLLTASALMGISFANKFIVLCLMAIPL
                     GLGGGAIDAALNNYVALHYKASHMSFLHSFWGVGATVSPLIMSYFLSQNGNWRGGYHT
                     VSIIQFALAAVMILSLPLWKRVPHAAVEQQKAEEPIVIGNREAIKQRGVLAAVLSFFF
                     YCAGETTTGLWAATYFVSVRGIEPSVAAGWGSLFFFGIMGGRMISGLLAMRVKEKTLV
                     RWGIGIAALGAVVLALPLPRAFQLAGLLLYGFGCAPIFPNLIHITPRRFGKDSSQAVI
                     GLQMAAAYVGSTLMPPIVGWVGQKAGFALLPYYLVACLLALSIGISVCNRKTGFETRA
                     "
     gene            3913..4260
                     /locus_tag="PLR57_00160"
     CDS             3913..4260
                     /locus_tag="PLR57_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39895.1"
                     /translation="MQLLIHITNHEDSVTPIFSKLLEAGISGATVVDCQGMLTTLSEC
                     SSVDAPPIFGSLRQFINPCRQTNKMIMIVLKDEDIPTVKEIIHSVAGNLKEPNTGIFF
                     TVPVMNWEGVSHK"
     gene            4262..5455
                     /locus_tag="PLR57_00165"
     CDS             4262..5455
                     /locus_tag="PLR57_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQQ39896.1"
                     /translation="MSTIFYIAIAMIAGLLLTRVVKLVKLPNVTGFLIAGLLIGPYCF
                     KLISVDMLDTLDILTTAALGFIAFSIGSEFKLAHIKAIGGKIILITVCEALGAVILVD
                     VAVIAFGFPVPMALTMGAIAAATAPAATLLVVRQYRAKGELTSTLLPVVAMDDAIGLM
                     AYAISVSVAKMIQNGDAFNVMTTIVDPLLEIVLSLAAGGVLGAVVAISNRFFQSKANR
                     LSISIAAVLLGASLAQKFDLSPLLLCMAIGAVYVNLRNDAIQTLEHVDTWTPPLFMMF
                     FVISGADLNVSMLPKLGLIGVLYIVARVIGKYFGAYLGCTISKTSPKIRKYLGFSLVP
                     QAGVAIGIAQLAVRELPQYGSSIQAVILCATLIYELIGPVLTKASLIKAGEIVVEKKQ
                     KQVAQ"
     gene            complement(5615..6592)
                     /locus_tag="PLR57_00170"
     CDS             complement(5615..6592)
                     /locus_tag="PLR57_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQQ39897.1"
                     /translation="MSRMRLLNESKGQSFEGFCIVKTVSIKTNVKGSDYLDLILADCE
                     GECAAKLWDYSKELHGTFEPNDVVKVRGTINIWKDTEQLKVDKIRHTTPEDDVDMALL
                     LPCAPYDPEWLYDQLFELAEAFSDDDLRRLVQYILRENREKLLFFPAAVKLHHATRGG
                     LLHHTWTLTQLAKAVIAIYPALDPDLVYTGAILHDIGKLDELETGELGIAGGYTTHGQ
                     LVGHISIGISRISAACEMLGVPSETCMLVEHMLLAHHGQAEFGSPKLPMFPEAEVLAE
                     IDLLDSKMYEMFDALEGVTVGAFSERQWALDNRQIYRHGHGLIKKGESK"
     gene            6794..>7518
                     /locus_tag="PLR57_00175"
     CDS             6794..>7518
                     /locus_tag="PLR57_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ39898.1"
                     /translation="MAVSFDQIYDIVVIGAGHAGVEAALASARMGKKTLILTLNLDSV
                     GLMPCNPAIGGTGKGHLVREIDAMGGEMGLAADDTLIQMRMLNTGKGPAVHSLRAQMD
                     KRRYHERVKRALESQPNLTLLQGEAKRFLTENGAICGVESAEGFVYGCRAAVVCTGVY
                     LKSRILIGSFVRESGPAGLLRAEYLTTALAELGIPIQRFKTGTPARVNGGTLDYSEME
                     RQDGDEPTPAFSFLHGRLSLEQTP"
BASE COUNT         1611 a         2042 c         2162 g         1703 t
ORIGIN      
        1 atgcgcaaag actgcatggc gcgaatggcg gaaatgcaaa aacgcctgca aaaacaagcc
       61 gtccgctgat aacggacggc tttttgcctg taacaatgcg tttgtgaagc gggcatgaac
      121 gaaaccggaa agatactgaa atgaatagta aaaccggtta caacgaaacc gcgtacaacg
      181 gcggctattc gggtttgttg gtatctgcct gttgttcagc agcgtcctct tcgggcggta
      241 catacagcgt ttgcgctgcg atgcggtcgc ccgcctcccg catggcgcgc ttcttctccg
      301 ttgctcggta aatcaaaaac cccgcgccgc tgagcagcgc ggttgcgcca aacaccacgg
      361 cggcgagcac ataccgctgc gtgccgagcg cgctgccgct gaccgtggag gtaatgatgg
      421 aaggcagtcg cgccacagag gtaatcagca cccatggcag cagacgaatc ttcgtcagcc
      481 ccgcggcgta agtaagcaag tccttgagcg tgccggggat aaaaaagagg acgaacgccg
      541 ttgcgttgag cgctttttcg ttgttcaaaa tgggcaggga gtcgattttt tcaagcggaa
      601 aaaccgcttc caccagcttg acgccgaatt tacgaacaaa gagaaatacg atcgtgcttc
      661 caagaatgag ccccacccaa acaagaagcg tgccttccag cgcaccgaag gcgtagccag
      721 cggcaagctc gaacggttcg ccgggaataa aagccacgac gacctgcagg atgcacatgc
      781 cgataaagat cagcttaccc caaacaccgc gcgcgtcgat ccagtcgcga aacgcggacg
      841 ggtctttgag cgtgctgatc agcggcttgc cgacgaacac ggtcaaaaag acgagcagca
      901 gcacgaacgt aacgattgaa acaatcgaaa cgatcttgcg cgttttcgaa gggggattga
      961 ctggctttgc tgtgttttgt tccatgcaaa tagtatagca tggtgaaatt cgggatgaat
     1021 agaacccgaa attgaacttt tggggaaatt ctgccgcacc ctgcgtcgaa tccagcgtat
     1081 tattttttta taatggggct tgcaatcaat ttttaatgcc agtatccttc gtttcaaaga
     1141 gcgctcttaa gatcaaacaa aggagatcaa acttcatgaa tcaaaatcag attgccgcgc
     1201 tggagcagac gattgcaagc cggtacggca atattgcggg cataattgtg ctgcgggatg
     1261 gcgaaaaaca gtatgagaac tacttccacg gcttcgacgc tggttccgcg tttcatgtgt
     1321 tttcgatcac aaagagcgtc ctttccatac tagttggcat tgccgttgac cgtgggcaga
     1381 tcgggagcgt cgagcagaag gtgctggatt tctttccgga ctacgtgcca aaacgaggcg
     1441 aacggacggc gcagcgcgtt acgatcaagg acatgctgtc gatgaccgcg ccgtttaagt
     1501 ataagtccgc gccctacacg aagtacttta cgagcgagag ctgggttaaa agcgcgctcg
     1561 acctgttggg cggcaagggc gagatcgggc agttccgcta tacgccgcta atcgggccgg
     1621 atattctttc gggcgttttg actagcacga ccgggcagtc cgcgctccat tttgcgcagg
     1681 aagcgctgtt tcagccgctc ggaatcgagg ttacgcaagc aatcacgttc caaagcaagg
     1741 aagagcagct ttctattatg aaacatcgcc acgaaagcgg ctgggtcgcc gacccgcggg
     1801 aatcaccacg gctggctggg ggctgtttgt aacgccagca gatatggcga acatcgacca
     1861 actttacttg aacaatggcg tttggaacgg caggcagatc gtctcctccg gctggattgc
     1921 tgagagcaca cgggaacaaa gccgctgcaa agaatggggc aatctcgcct atggttacct
     1981 ctggtggctg atcgacgaaa acagctttgc agcgctcggc gacggcggaa acgtgatcta
     2041 tgtgaaccga aagaatcggc tggtggtcgc catcgcgtcg ctattcaagc cgaatgccgc
     2101 ggatcgcatc tcgctgatcc ggcgggagat cgagccgtta ttcggcggcg cgcgataacg
     2161 cgactcgtgc agttaagcag agtatctgct gcgggatgtg tcagagaatc ttgtgtggcg
     2221 tctaaggaaa gcctgaaagg aacctgaaag cattttttca gctagaattc agcgtagagg
     2281 ggtattctta tggtgttgaa accaatcaag tcaaccggcc ggtgaccggg acgactgaaa
     2341 gggacagcgg caggactttt tcattttttt cctccttttt gcaaacgccg cggtttgggc
     2401 cccgcggcgt tttgctgtcc gcagaggatg ccggtttccg tgttcaggaa attcctgcaa
     2461 gtatccaacc gattaccgta attaaaacgc cgtgcgcgcg tatttctctt ttcaagcgga
     2521 tttgttttcg gtacaatacg cgcatggcta caattttatt gattattatc tatctggcgt
     2581 tcatcagttt gggattgccg gattccctgc tcggcgcggc ctggccggtc gcgtacgcgc
     2641 agcttggcgc accggtctcc tacgcgggac ttgtctctat ctttataacg gtaggaacgg
     2701 tcatctccag cctcgtctcc gtccgcgtga tccgaaagtt cggtaccggc gcggtgacgg
     2761 cgttctgcgt gctcctgacc gcgagtgcgc tgatgggcat atcgtttgcc aacaagttca
     2821 tcgtgctctg cctgatggcc atcccgcttg ggcttggcgg cggggcgatc gacgcggcgc
     2881 tgaataatta cgtagctctc cactacaaag ccagccacat gagctttttg catagcttct
     2941 ggggcgtcgg cgctaccgtc agcccgctga ttatgtcgta cttcctctcg cagaacggaa
     3001 actggcgcgg cgggtaccat acggtttcca tcatccagtt tgcgctcgcg gcggtgatga
     3061 ttctttcgct tccgctctgg aagcgcgttc cgcatgccgc cgtggagcag cagaaagcgg
     3121 aggagccgat cgtcatcggc aaccgcgaag caatcaaaca gcgcggcgtt cttgcggcgg
     3181 tactttcgtt ctttttctac tgtgcgggtg aaacgaccac cgggctgtgg gcggcgacgt
     3241 actttgtcag cgttcgcggt attgagccga gcgttgccgc gggctggggt tcgctgttct
     3301 ttttcggcat catgggcggg cgtatgatct ccggcctgct ggccatgcgt gtcaaggaaa
     3361 agacgctcgt gcgctggggc atcggcatcg ccgcgcttgg cgcggttgtg cttgcgcttc
     3421 cgctgccgcg cgccttccag ctcgcggggc tgctgctcta tggctttggc tgcgcgccca
     3481 tcttcccgaa tctgattcat attacgcccc ggcgtttcgg aaaagacagc tcacaggcgg
     3541 tcatcggcct gcagatggcg gcggcgtacg ttggcagcac gctcatgccg ccgattgtcg
     3601 gctgggtcgg gcaaaaagcg ggatttgcgc ttttgccgta ttatctggtt gcgtgcctgc
     3661 tggcgctgtc tatcggaatt tccgtctgca acaggaaaac agggtttgaa acacgcgcgt
     3721 aactgccgca ttcttagcca aatcagttga taaaacaaga gccgcgcgtc tgtagaaagg
     3781 cgtgcggctc ttgacaaatg cgatttatga gcgtaatttt catgtgttag agaaaaaaca
     3841 tgggaaaaac gcccgcgtat accggcaaaa tacgccaaat tacgcggtcg ggacggaaaa
     3901 ggagatgggt gcatgcaatt attgattcac attaccaatc atgaagacag cgtcacgccg
     3961 attttttcaa aacttttaga agcgggaatt tcgggtgcaa ccgttgtgga ctgtcagggg
     4021 atgctcacta cgctcagcga atgcagcagc gtggacgcgc cgccgatttt cggctcgctg
     4081 aggcagttta tcaacccctg ccgccagacc aacaagatga tcatgatcgt tctcaaggat
     4141 gaggatattc caactgtcaa agagatcatc cacagcgttg cgggcaacct caaggagccc
     4201 aacaccggca ttttctttac cgtgccggtc atgaattggg aaggagtctc gcacaagtaa
     4261 catgtctacc atattttata tcgcaatagc tatgattgcg ggccttctgt tgacgcgcgt
     4321 ggtcaagctg gtgaaactgc ccaatgttac cgggtttctg atcgcgggct tgctgattgg
     4381 tccatactgc ttcaaactga tctcggtcga tatgctggat acgctggata tcctcaccac
     4441 cgccgcgctc gggttcattg cgttctccat cggcagcgaa ttcaagctcg cgcatatcaa
     4501 ggccatcggc ggaaagatta ttctgatcac cgtttgcgaa gcgctcggcg ccgtgattct
     4561 ggtggatgtg gccgtcatcg cgttcggctt ccccgtaccc atggcgctga ccatgggtgc
     4621 tatcgccgcc gccaccgcgc ccgccgccac gcttctcgtc gtgcggcagt accgcgccaa
     4681 aggcgagctg accagtacgc tgctgcccgt cgtcgccatg gacgacgcca tcgggctgat
     4741 ggcctacgcc atctccgttt ccgttgccaa gatgattcaa aacggggacg cgttcaacgt
     4801 tatgaccact atcgtcgacc cgctgctgga gatcgttctt tcgttggctg ccggcggcgt
     4861 gcttggcgcg gttgtcgcaa tttccaaccg ctttttccag agcaaagcca accgcctcag
     4921 catcagcatt gcggcggtac tgctcggcgc gagccttgcg cagaagtttg acctttcgcc
     4981 gcttttgctg tgcatggcca tcggcgcggt atacgtcaac ctgcgcaacg acgcgattca
     5041 gaccctcgag catgtggaca cctggacacc gccgctgttt atgatgttct tcgtcatctc
     5101 cggcgcggat ctgaacgtat ccatgctgcc gaagctcggc ttgatcggcg ttctgtatat
     5161 cgtagcccgc gttatcggca agtactttgg cgcgtacctt ggctgcacga tttcgaaaac
     5221 ctcgccaaag attcgcaagt accttggctt ctctctggtt ccgcaggcgg gcgttgccat
     5281 cggtattgcg cagctggcgg tgcgtgaact gccgcagtac ggctcttcca ttcaggccgt
     5341 cattctctgc gctacgctga tctacgaact gatcgggccg gtgctcacca aggcttcgct
     5401 catcaaggca ggcgaaatcg tggtggaaaa gaagcagaaa caggttgcgc aataacggat
     5461 tttctttttc ttgaaagaaa aagaaacaaa aagaacttcc aaaccaattt acttttcttg
     5521 aaagaaaagt aacaaaagaa cttctcgctt tatacacgag aagttcttta tttatacgtc
     5581 ttttgcttct tttcttcaga aaagaagagt tcccttactt actttcccct tttttaatca
     5641 gcccgtgtcc gtggcgatag atctggcggt tatccagcgc ccactggcgt tcggagaacg
     5701 cacccacggt aacgccttcg agcgcgtcga acatttcgta catcttgctg tccagcaagt
     5761 cgatttccgc cagcacttcc gcttccggga acatcggcag tttgggactg ccgaactccg
     5821 cctgcccgtg atgggcaagc agcatgtgct ccacgagcat gcaggtttca ctcggaacgc
     5881 ccagcatttc gcaggcggcg ctgatacggg aaatgccgat ggagatatgc ccgaccagct
     5941 ggccgtgcgt ggtgtagccg cccgcaatgc cgagctcgcc ggtttcaagc tcgtcgagct
     6001 tgccgatatc gtgcagaatc gcgccggtgt agacgaggtc gggatcgagc gcgggataga
     6061 ttgcgatcac ggctttggcg agctgcgtca gcgtccaggt gtggtgcaga agtcccccgc
     6121 gggtggcgtg gtgcagtttg acggcggcgg ggaagaacaa aagcttttcc ctgttttcgc
     6181 ggaggatata ctgcacaagc cgcctgaggt cgtcgtccga gaacgcctcc gccagttcga
     6241 agagctggtc gtagagccat tcgggatcat acggcgcgca gggtaaaagc agcgccatgt
     6301 ccacgtcgtc ctccggcgtg gtgtggcgga ttttatccac cttgagctgc tcggtgtcct
     6361 tccagatatt gatcgtgccg cgcaccttga ccacgtcgtt cggttcaaac gttccatgca
     6421 gttccttgct gtagtcccag agcttggcgg cgcactcgcc ctcgcagtcc gccaggatca
     6481 ggtcaaggta atcgcttcct ttgacgttgg ttttaatgga aacggtctta acgatgcaga
     6541 atccctcaaa agactgaccc ttgctctcgt tcagcaggcg catgcggctc atggaaaagt
     6601 ccccctttgg ataattgaaa ccggttggtt tttttcagta taccacaaac atagtgcact
     6661 ggcgaaaaaa gcgaatgacc cgcgcggaag cgtaaaatcc cgcccgcttt gcccgcgcgt
     6721 tgcggcaatt gagcggatgt gatagaatta cgcccgtatc aacgggatat ttcccaaaaa
     6781 gacaggcgga aacatggcag tttcattcga ccagatttac gatatcgtcg tcatcggcgc
     6841 ggggcacgcg ggggtggaag ccgcccttgc ttcagctcga atggggaaaa agacgctcat
     6901 tttaacctta aatctcgact cggtggggct catgccctgc aatccggcca tcggcggcac
     6961 cggaaagggt catctcgtgc gggaaatcga cgccatgggc ggggagatgg ggcttgccgc
     7021 ggacgatacg ctgattcaaa tgcgcatgct caacaccggc aaaggccccg cggtgcattc
     7081 gctccgcgcg cagatggata agcgccgcta ccacgagcgc gtcaagcgcg cgctggagag
     7141 ccagccgaac ttaacgctcc tgcagggcga agccaagcgg tttttgaccg aaaacggtgc
     7201 aatctgcggc gtggaaagcg cggaaggttt tgtttacggc tgccgggcgg ctgtggtctg
     7261 cacaggcgtt tacctcaaaa gccggattct gatcgggagc tttgtgcgcg agagcgggcc
     7321 ggcggggtta ctgcgcgcgg agtatcttac cacggcgctt gccgagcttg gcatcccgat
     7381 tcagcggttc aagaccggaa cacccgctcg cgtcaacggc ggaacgctgg actattcgga
     7441 aatggagcgg caggacggcg acgagccgac gcccgcgttt tcgtttttgc acgggcggct
     7501 ttcgctggag cagacgcc
//
LOCUS       DAOMGY010000005         6229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_12713_length_6229_cov_3.616294, whole genome shotgun sequence.
ACCESSION   DAOMGY010000005 DAOMGY010000000
VERSION     DAOMGY010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12713_length_6229_cov_3.616294"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PLR57_00180"
     CDS             complement(<1..878)
                     /locus_tag="PLR57_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HQQ39899.1"
                     /translation="MSKLNELFNQKLHVVNFGIESFYDDLKGQGEAAVHVDWKPVAGG
                     DKAAGANLRKLKAPEFAAKIEAANKEALRRILAAQPAIVGLGIAGEEIPGMTKTTILH
                     AGPPVTWERMSGPQRGAVMGGLIYEGLAKDLTEAEKLAASDAIKFDPCNMHSAVGPMA
                     GVVTYSMPVWILENKAFGNRAYCTINEGLGKVLRFGACDEEVLTRLRWMEKELYPVLK
                     EAMAIKKEIDVKVLVAKLLQMGDEGHNRNEAGTSLLIRELVPAILACSKPQEQKVAAY
                     NFINGNNHTFLNISMPA"
     gene            complement(871..2454)
                     /gene="fdrA"
                     /locus_tag="PLR57_00185"
     CDS             complement(871..2454)
                     /gene="fdrA"
                     /locus_tag="PLR57_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HQQ39900.1"
                     /translation="MVQLEIRKNAYYDSVTLMLISKELKKVAGVTEALVGMGTDLNVE
                     IAHNIGVATPEFDKQVTANDFFVAVECKDEASFAAAMKKVEELLNKKTEAKASSYSPP
                     TLEGALKIDPALNMAVISVPGRHAADVAQGCLDHGVNVMLFSDNVTIEEERALKQFAY
                     ERELLVMGPDCGTAIINNVPLAFANVVRAGDIGVVAASGTGTQEVTSLIDQLGGGVSQ
                     VIGTGGRDLKKEIGGLMMKLGLDALKNDPKTKVIVLISKPPAKEIATEILNIAAKAGK
                     PVVVSFIGGDRKEAEAFGLVAAVSLEDAAHKAVVLSKGAKPEDFTSFSMGQAKAEALA
                     KEIAGKMAKGQKYIRGFYTGGTLCDEAMKLLIGSLGHIYSNIPLNAEDKLADAKGGKS
                     REHTFLDFGDDEFTVGRPHPMIDPSLRAERVITDGKDAECAVILCDCVIGYGSHPNPA
                     EDLALSIKAAKAEAAAAGRHLCCVCAVCGTQGDPQSLTRTQQQLEEAGAVVLPSNAQA
                     ARFALLVLANLRQEGPDHE"
     gene            complement(2458..3369)
                     /locus_tag="PLR57_00190"
     CDS             complement(2458..3369)
                     /locus_tag="PLR57_00190"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HQQ39901.1"
                     /translation="MERGGLKLTLTADAVCSRLLKRMREKPVTATVHSVFDHAVNLAF
                     ENRFGLVGLIAGEKTLTPYAVSVQTPCSFTELGFRAGMAAAVQAETIELPEAGVLIDC
                     SRTKPIDLGIGSIRFDTPAGLKQAFPVLRQTLEQTDAEVCLAPLVTGGAGNVYTNFLA
                     PRLEELVQAVAAGDAKRAANAAANAAGCGMGLTPSSDDLLTGYFAGLHAIFRTESKGE
                     RISLIPIMAQRAAEKTNRISATFLLQSGEGLVNYDLLLLIQSIVTGADSTAIKQAAQK
                     VLSIGSTSGGDMLTGLSLALAHHAGGN"
     gene            complement(3376..3741)
                     /locus_tag="PLR57_00195"
     CDS             complement(3376..3741)
                     /locus_tag="PLR57_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HQQ39902.1"
                     /translation="METMYAYKCKKCGKLHHPRYIVCQNPDCDGREFEQVPLEGKCKL
                     LTWTRVYNLPEGYMQPWRNFGIVEFENGVRVTGQIGFDDGIESGMELVSTVGVVRTRR
                     ILYKETIDCEEYGFVFQKA"
     gene            complement(3754..4950)
                     /locus_tag="PLR57_00200"
     CDS             complement(3754..4950)
                     /locus_tag="PLR57_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HQQ39903.1"
                     /translation="MRQVGIVGVGHTKFGKSSVPFIDMLCEAALQAMDDAGAKTGRRN
                     VIDQVFVGSMGSGMVSRISGAASAVVDTLNIRPAMAETVENGPASGASAVKLGVMAIA
                     SGMCDCVLVIGGEGMREVSGWEGTDFVATMLHPEAEYKYGCTLPSFAGLFTRLCMDKF
                     GVESKDLSIVSVKNHDNACHNPVAHIQRPVVLERITNPAYINATNPIVADPLRQFDMC
                     PVSDGAAAVLLVAQDKQEALGFGHKKFVRVAGIASATDTHVVANRAEPSDLLAVRLSA
                     NKAYAMAGMKPEDIDVAELHDAFQILEIEESEEVGFFPRGEGHLAARRGDTKIGGKIP
                     VNTSGGLKAKGHPLGATGVSQIVELVRQLRGEAEGRQVEGAKTGLAINFGGFGNNVVA
                     TILTTK"
     gene            complement(4981..5652)
                     /locus_tag="PLR57_00205"
     CDS             complement(4981..5652)
                     /locus_tag="PLR57_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39904.1"
                     /translation="MSKYDIPVDEMEAKLRPPTIGLKPYEPKVIPLTTGEDMLVREAT
                     REETAMVLEGIKVLLDHEKDFYDIVSARIVAELLGMLRYRVQDEYLLIGCVDGEIAGI
                     VNGRMVNEKIGMSYHTMTLKRGARVGAQLFAAKMEYHFDYMNQDEVWIVAESPNGFKR
                     WMIEYELESRPQCPHELGGVPTFVLTRALWEKHKANKNVGIRPAFEDVIEANKVVHMP
                     AKIVV"
     gene            complement(5791..>6229)
                     /locus_tag="PLR57_00210"
     CDS             complement(5791..>6229)
                     /locus_tag="PLR57_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39905.1"
                     /translation="AFMPGASPPLVRMPIRRILSGIGFSSILSISGIAALKSKTLPRL
                     VYYTRRKNSNSAQIQQNSVITQERSKNPAILIDILLYKWRKGRPISVAISQERCTLQG
                     KMRRLMRTKNTRIGRKYMIIYRFTINLCGFITVYAPHAKLQGL"
BASE COUNT         1135 a         1875 c         1750 g         1469 t
ORIGIN      
        1 gcgggcatgg agatgttcag gaacgtatgg ttgttgccgt tgatgaagtt gtaggcggca
       61 accttctgct cctgcggttt gctgcaggcc agaatcgcgg gcacaagctc gcgaatgaga
      121 agcgacgttc ccgcttcgtt gcggttgtgg ccttcgtcgc ccatctgcag gagtttggca
      181 acgagcactt ttacgtcgat ctctttctta atcgccatcg cttccttgag caccgggtag
      241 agctccttct ccatccagcg cagtcgggtg agcacttcct cgtcgcatgc gccaaagcgg
      301 agcaccttgc cgaggccttc gttgatggtg cagtacgcgc ggttgccgaa cgccttgttt
      361 tcgaggatcc agaccggcat gctgtaggtg acaacgcccg ccatcgggcc caccgcgctg
      421 tgcatgttac aggggtcgaa tttgattgcg tcgctcgcgg cgagcttttc tgcttccgtc
      481 aaatccttgg cgagcccttc gtaaatcagg ccgcccatga ccgcgccgcg ctgcggaccg
      541 ctcatgcgtt cccaggtcac cggcgggccc gcgtgcagaa tcgtagtttt tgtcataccg
      601 ggaatctctt cgcccgcaat gccgagaccg acgatggccg gctgcgcggc gaggatgcga
      661 cggagcgctt ccttattggc ggcttcgatc tttgccgcga actccggcgc tttcagcttg
      721 cgcaggttcg cgccggcagc tttgtcgccg cccgcaacgg gcttccagtc cacgtgaacc
      781 gccgcttcgc cctgaccctt gagatcgtcg taaaacgact cgatgccgaa gttcacgacg
      841 tgcagttttt gattgaataa ttcgttcagt ttactcatga tcgggtccct cctggcgcag
      901 gttggcaagc acaagcagcg caaagcgcgc cgcttgcgcg ttgctcggca gcaccaccgc
      961 gcccgcttct tcgagctgct gctgcgttct tgtcagcgac tgcgggtcgc cctgcgttcc
     1021 gcagaccgcg cagacacaac agagatgccg tcccgcggcg gcagcttccg cttttgcggc
     1081 cttaatcgac agagcaaggt cttccgcagg gttcgggtgc gaaccgtagc caatgacgca
     1141 gtcgcagaga atcaccgcgc attccgcgtc ttttccgtcg gttattacgc gctcggcgcg
     1201 caggctcgga tcgatcatcg ggtgcggtcg tccaacggtg aactcgtcgt ccccgaaatc
     1261 gaggaacgtg tgctcgcggc tcttgccgcc cttggcgtcc gccagtttat cctccgcgtt
     1321 gagcggaatg ttgctgtaaa tatgccccaa tgagccgatt aagagcttca tggcttcgtc
     1381 gcagagcgtg ccgccggtgt agaacccgcg gatgtacttc tgcccctttg ccatcttgcc
     1441 cgcaatctct tttgccagcg cttcggcctt cgcctgcccc atggaaaacg aggtgaaatc
     1501 ctcgggcttt gcgcctttgc tgagcacgac ggccttatgc gccgcgtcct ccagcgaaac
     1561 cgccgcgacc agcccgaagg cttccgcttc cttgcggtcg ccgccgataa agctcacgac
     1621 aaccggcttt ccggctttcg cggcaatatt caatatctcg gttgcaatct ccttggccgg
     1681 gggcttgctg atgagcacga tcacctttgt tttggggtcg ttcttcagcg cgtcgaggcc
     1741 aagtttcatc atcagcccgc cgatttcctt tttcaggtcg cgcccgccgg tgccgattac
     1801 ctgactgacg cccccgccga gctggtcgat caggctcgtg acttcctgcg tgcccgtgcc
     1861 ggaagcggcg acaacgccga tatcccccgc gcgcacgacg ttggcaaacg cgagcggcac
     1921 gttgttgata atcgccgttc cgcagtcggg ccccatcacg agcagttccc gctcgtaggc
     1981 gaactgtttg agcgcgcgct cttcttctat cgttacattg tccgaaaaca gcatcacgtt
     2041 tacgccgtga tcgaggcacc cctgcgccac gtccgctgcg tgccgtcccg gaacggaaat
     2101 gaccgccata ttcagcgcgg ggtcaatctt cagcgcgcct tcgagcgtcg gcgggctgta
     2161 ggaggaagcc ttcgcttccg tctttttatt gagcagctct tccacctttt tcatcgccgc
     2221 ggcaaaggac gcttcgtcct tgcactccac ggcgacaaag aagtcgttgg ccgttacttg
     2281 cttgtcgaat tcaggcgtcg caacgccgat gttgtgcgca atctcaacgt tcaggtcggt
     2341 acccatgccg accagcgctt ccgtgacgcc cgcaaccttt ttgagctctt tggagatgag
     2401 catcagggtc acggagtcgt aatatgcgtt tttacgtatt tccagttgaa ccacttttca
     2461 atttcctcct gcgtgatgtg ccagtgcgag cgacagtccc gtgagcatgt ccccgccgga
     2521 cgtcgagccg atggacagaa ccttttgcgc ggcttgtttg atagcagtgc tgtccgcgcc
     2581 ggtaacgatg gattggatga gcagcagcaa gtcgtaattg acgagccctt cgccgctttg
     2641 cagtagaaat gtcgcgctga tgcggttcgt tttttccgcc gcgcgctgcg ccataattgg
     2701 aatgagcgaa atgcgctccc ctttgctttc cgtacggaag atcgcatgga gtccggcaaa
     2761 gtaccccgtc aacaggtcgt ccgaggacgg cgtcagtccc attccgcagc ccgccgcgtt
     2821 cgccgcggcg tttgccgcgc gtttggcgtc tcccgccgct accgcctgca cgagctcttc
     2881 aaggcggggc gcaaggaagt tcgtatacac gtttcccgcg ccgcccgtta cgagcggagc
     2941 gaggcaaact tccgcgtccg tttgttcaag cgtttgccga agaaccggaa acgcctgctt
     3001 cagtccggca ggggtatcga accggatcga accgatgcct aggtcgatcg gtttggttcg
     3061 ggagcaatcg atcaatacgc ccgcttcagg cagttcgatc gtttccgcct gtaccgccgc
     3121 cgccatgccc gcgcgaaaac ccagttcggt aaacgaacac ggtgtttgca cggacacggc
     3181 gtacggcgtt aatgtctttt cgcctgcgat caggccgacg agcccgaacc tgttctcaaa
     3241 cgcaaggttc accgcgtgat cgaaaacgga atgcaccgtt gccgtcacgg gcttttcccg
     3301 catgcgcttg aggagccggg agcaaaccgc gtcagcggtc agcgtcagct tcagaccacc
     3361 gcgttccaag cgaggttacg ccttttggaa tacgaagccg tattcctcgc agtcgatggt
     3421 ttctttgtag aggatgcgcc gcgtgcgtac tacgcccacg gtggagacca gctccatgcc
     3481 gctctcgatg ccgtcgtcaa agccgatctg gccggtcacg cgaacgccgt tttcaaactc
     3541 cacaatgccg aagttgcgcc agggctgcat gtagccttcc ggcaggttgt aaacgcgcgt
     3601 ccaggtcagc agcttgcact tgccttcgag cgggacttgt tcgaactcgc gcccgtcgca
     3661 atccggattc tgacagacta tgtaacgagg atgatgcagc tttccgcatt ttttgcactt
     3721 gtatgcgtac atggtttcca tattctctcc tccttacttc gtggtcagaa tcgtcgccac
     3781 gacgttgttg ccaaagccgc cgaagttgat ggcgaggccg gttttcgcgc cttccacctg
     3841 acggccttcg gcttcgccgc gcagctgacg aaccagctcc acaatctgcg aaacgccggt
     3901 tgcgcccagc ggatgccctt tggctttcag gccgccggag gtgttgaccg gaatcttgcc
     3961 gccaatcttc gtgtctcccc tgcgggcggc cagatggcct tccccgcgcg ggaagaagcc
     4021 gacttcttcg gactcttcaa tctccagaat ctggaacgcg tcgtgcagtt cggcaacgtc
     4081 gatatcctca ggtttcatgc cggccatggc atacgcctta ttcgcgctca ggcgaacggc
     4141 cagcaggtcg ctcggttccg cgcggttggc aaccacgtgc gtatccgttg cggaagcgat
     4201 gcccgcaacg cgtacgaact tcttgtgccc aaagccaagc gcttcctgct tatcctgcgc
     4261 aaccagaaga accgccgccg ctccgtcgga aacggggcac atatcgaact gccgcagcgg
     4321 gtcggccacg atggggttgg tggcgttgat gtaggcgggg ttggtgatgc gctccagcac
     4381 gaccgggcgc tgaatatgcg caacggggtt atggcaggcg ttgtcgtggt ttttgacgga
     4441 tacgatcgaa agatccttgc tctccacgcc gaacttatcc atgcagaggc gcgtaaacaa
     4501 gcccgcgaac gacggcagcg tgcagccgta tttgtattcc gcttccggat gcagcatggt
     4561 ggcaacaaag tccgtgcctt cccagccgct cacttcgcgc atgccttcgc cgccgatgac
     4621 cagtacgcag tcgcacatgc ctgaggcaat ggccataacg cccagcttca cggcggaagc
     4681 gccggatgcg gggccgtttt ccacggtctc cgccatcgcg gggcgaatgt tgagcgtatc
     4741 gacaaccgcg ctggcggcgc cgctgatgcg gctgaccatg ccggagccca ttgagccgac
     4801 aaacacctga tcgatcacgt ttctgcggcc ggttttcgcg cccgcgtcgt ccattgcctg
     4861 aagcgccgct tcgcaaagca tatcgatgaa cggaacgctg gatttgccga acttcgtatg
     4921 gccgacgccg acgattccta cctgtctcat ttcaagtatc ctttctggta cgcgccgttc
     4981 tcagacgacg atcttggcgg gcatatgcac cactttgttg gcttcgatca cgtcttcaaa
     5041 cgcgggacgg atgccaacgt tcttgttcgc cttgtgcttt tcccagaggg cgcgggtcag
     5101 aacgaacgtg ggtacgccgc cgagctcgtg cgggcactgc ggacgggatt cgagttcgta
     5161 ctcgatcatc cagcgcttga agccgttggg gctttccgca acgatccaga cttcgtcctg
     5221 gttcatatag tcgaaatggt attccatctt ggcggcaaag agctgtgcgc caacgcgcgc
     5281 gccgcgcttg agggtcatgg tgtggtagct catgccgatc ttttcgttga ccatgcggcc
     5341 gttgacgatg ccggcgattt cgccgtccac gcagccgatg agcaggtatt cgtcctgcac
     5401 gcggtagcgc agcatgccca gcagttccgc aacgatgcgc gccgaaacga tatcgtaaaa
     5461 gtctttttcg tggtcgagga gaaccttaat cccttccagc accatcgcgg tttcttcacg
     5521 cgtcgcttcg cgaaccagca tatcctcgcc ggtggtcagc ggaatcactt tgggttcata
     5581 gggtttcaat ccgattgtcg gcggcctgag tttggcttcc atttcatcta cggggatatc
     5641 atacttgctc atgtgtggtg cctccttatt gttctgtttg gtttaggatc gttaaaaaaa
     5701 gatatgtcgg ttccggtgtt tgtacgccgg ttacaaaatc attgtagcgt cagaatgaaa
     5761 tcttgtctag tatttcttgc aaaatatttt ttaaagtccc tgcaattttg cgtgcggcgc
     5821 gtacactgtt atgaatccgc atagatttat tgtaaaacga taaataatca tatattttcg
     5881 cccgatacgg gtgttttttg tacgcatcag acgacgcatt ttcccctgca aggtacagcg
     5941 ctcttgtgaa atcgcgacag aaatcggcct gccttttcgc catttataca acagaatgtc
     6001 gattaatatt gcaggatttt tgcttctttc ctgcgttatt acagaatttt gttgaatttg
     6061 cgcgctgttt gagtttttcc ggcgcgtata gtatactaat ctgggtaaag tcttagactt
     6121 caacgcggca atgccgctta tagaaaggat cgaagaaaaa cctatgccag atagaattcg
     6181 tcgaatcggc atcctcacca gcggcggcga cgcgccgggc atgaatgcg
//
LOCUS       DAOMGY010000006         6215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_12758_length_6215_cov_3.557468, whole genome shotgun sequence.
ACCESSION   DAOMGY010000006 DAOMGY010000000
VERSION     DAOMGY010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12758_length_6215_cov_3.557468"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /gene="rpsB"
                     /locus_tag="PLR57_00215"
     CDS             <1..465
                     /gene="rpsB"
                     /locus_tag="PLR57_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQQ39906.1"
                     /translation="VNQRWLGGMLTNFKTIQGRIARLRQIEQMEENGDFELLPKKEVI
                     QLRAEREKLEKNLGGIKDMKKLPAALFVVDPRKEHIAVSEARTLKIPIVAIIDTNCDP
                     DEVDYPIPGNDDAIRAVKLITAKIADAVIEGHQGEQMTDTAEAAEAEVEEEA"
     gene            493..1416
                     /gene="tsf"
                     /locus_tag="PLR57_00220"
     CDS             493..1416
                     /gene="tsf"
                     /locus_tag="PLR57_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQQ39907.1"
                     /translation="MFTAKDVMELRELTQAGMMDCKKALTECDGDFEKAKDYLREKGL
                     AAAAKKAGRIAAEGIVGGFVDPKTGVGVIVEVNCETDFVAKTDNFKDFVAMVAEQVAQ
                     KNPADVDALLAQDCISTPGTTISGMLNEAVLKIGEKISIRRFARYEGVVDCYIHLGGR
                     IGVLTEMDAPANLKDNDTFKTVAHDISMQIAAARPTYLKREDVPAEEVEHEKEINRAM
                     ALNEEKPKPAEVIEKMLVGRIEKFYKEVCLLEQLFVKDTSMNITKLLENTSKELGAKI
                     TVKRFARFEMGEGLQKRECNFAEEIAAATKH"
     gene            1547..2239
                     /gene="pyrH"
                     /locus_tag="PLR57_00225"
     CDS             1547..2239
                     /gene="pyrH"
                     /locus_tag="PLR57_00225"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQQ39908.1"
                     /translation="MYHRVLIKVSGEALSSEQFVICRETVLKTANMLKAIHKLGVQIG
                     VVVGGGNIMRGRSAEGMERNRADHMGMLATAINALALQDALEGIGVPCSVQSAVEMER
                     FCDSFSSRAARSALEEGKVVIFACGSGCPFFSTDTAAALRAAEIDADALLLAKNVDAV
                     YSADPKTDPKAVKYSQITYDRVVAENLHATDLTAITLCKEQNIPIRVFALSQIEEVFQ
                     DDSIGTHILANA"
     gene            2394..2951
                     /gene="frr"
                     /locus_tag="PLR57_00230"
     CDS             2394..2951
                     /gene="frr"
                     /locus_tag="PLR57_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQQ39909.1"
                     /translation="MADVMIDSTKERMNKTIAVLKSELGGLRAGRANPQLLERILVDY
                     YGTPTPIPQLGNISTPEARMLVIAVWDQKAIPAIEKAIQKSDLGINPSNDGKVIRLLF
                     PELNEERRKDLVKVVHKKGEECKVAIRSIRRDVMEQIKKDCKSSTITEDDQKYLEEKI
                     QKLTDDMIKEIETILAAKEKEILAV"
     gene            2948..3157
                     /locus_tag="PLR57_00235"
     CDS             2948..3157
                     /locus_tag="PLR57_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39910.1"
                     /translation="MTSVLGYPLDRAIEVLQSEGFCVKTEEARSKKGVLDGKEQRVIR
                     QSEPIENNVTLVYALFKTEPNETND"
     gene            3205..>3748
                     /gene="uppS"
                     /locus_tag="PLR57_00240"
     CDS             3205..>3748
                     /gene="uppS"
                     /locus_tag="PLR57_00240"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HQQ39911.1"
                     /translation="MRTYTDEQLQSLGLSAAALPRHVAIIMDGNGRWAKSRRLPRSAG
                     HRAGTDRLRGIIRLSSDLGIEALTLYAFFTENWKRPEDEVGTLMGLLVEYFTNEIDEL
                     DQNRVQIRIIGDTTRLPTRAKNAVDRAMARTGSNDGLKLSIALNYGSRAEILLAANKA
                     AKAANEQGLDEIPQELFESFL"
     assembly_gap    3749..3758
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3759..3962
                     /locus_tag="PLR57_00245"
     CDS             <3759..3962
                     /locus_tag="PLR57_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HQQ39912.1"
                     /translation="KDLPEVDLVIRTSGEQRISNFLLYQISYAEFLFPKEYWPDFSDE
                     CYINALRDFSGRARRYGGLGEEA"
     gene            3959..4774
                     /locus_tag="PLR57_00250"
     CDS             3959..4774
                     /locus_tag="PLR57_00250"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQQ39913.1"
                     /translation="MKTRVIVGILLVLMLIAVLFFGGFLLLSVLSLFSLAAIYEMGMT
                     FRKKGYNPIVLPAYIFAVSYSFVYYYFGLLAMIIFYMATVMATMIYSLFTKVHSTNDI
                     IPSLFIMNYPTLFLMCMMLVYFNFDRTTALVAAGMAYAAPECADTFAYFGGTLLGKHK
                     LCPTISPKKTVEGAAFALLGGIAFGFGMYYLQRLWGAAINPIVLVSLGLGCGLLSQFG
                     DLFASTIKRWAGVKDFSSVFPGHGGIIDRIDSILFCAPLVLCVFVVLRQLAVL"
     gene            4835..5989
                     /gene="dxr"
                     /locus_tag="PLR57_00255"
     CDS             4835..5989
                     /gene="dxr"
                     /locus_tag="PLR57_00255"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQQ39914.1"
                     /translation="MTKKITVLGSTGSIGRQTLDVISSQPEEMTLFGLSARSDITSLI
                     EQAILYRPQIVASETPFDPSLLPDGTETIFGDGASTVLAARTEADVVVNGVSGFAALA
                     PLLAALEHGKRVALANKESIVCGKQLVDETKRRFGGEIIPVDSEQSAIFQCLQNGRAE
                     EVERLILTASGGPFWRLDRPALEQVTLEQALSHPTWNMGKKITIDSATLFNKGLEVIE
                     AAYLFDLPGEQIEVLIHPQSIVHSMVAYRDGTCMANMSRPDMRLPIQYAITYPARNGS
                     VCKPLLLQNEKPLEFFEPDLARFGALQLAYQTLAGGKSYPIAYNGANEAAVEAFCGGL
                     IGFMDIERVVDYTLQEHSPVIAATLEEIVEADRAVRVLANSRIERIARER"
     gene            6000..>6215
                     /locus_tag="PLR57_00260"
     CDS             6000..>6215
                     /locus_tag="PLR57_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQQ39915.1"
                     /translation="MSTVLSIFVALLLLSLLVMIHELGHFLAGRALGFSILEFAIGMG
                     PVLWKTTRNGIQYSIRALPIGGMCRFNG"
BASE COUNT         1483 a         1642 c         1780 g         1300 t
ORIGIN      
        1 gtcaaccagc gctggctcgg cggcatgctc accaacttca agaccatcca gggccgcatt
       61 gcgcgtctgc gtcagattga gcagatggaa gaaaacggcg atttcgaact gctgcccaag
      121 aaggaagtca ttcagctccg cgccgaacgc gaaaagctcg aaaagaacct cggcggcatc
      181 aaggatatga agaagcttcc cgccgcgctg ttcgtggttg atccgcgcaa agaacacatt
      241 gccgtttcgg aagcgcgcac cctcaagatc ccgatcgtcg ccattatcga cacgaactgc
      301 gatccggacg aagtcgatta cccgattccg ggcaatgacg acgctatccg cgcggtcaaa
      361 cttatcaccg cgaagattgc cgacgccgtg atcgaaggac accagggcga gcagatgacc
      421 gataccgccg aagccgcgga agcggaagtg gaagaagaag cgtaattcgt tttcggaagg
      481 agaaaaattc gtatgtttac agcaaaagac gtaatggagc tgcgtgagct gacgcaagcc
      541 ggcatgatgg actgcaaaaa ggccctcacc gagtgcgacg gcgattttga aaaagccaag
      601 gactatctgc gcgagaaggg tctcgcggca gccgccaaga aggccggacg catcgcggcg
      661 gaaggtattg tcggcggatt tgtcgatccg aaaaccggcg ttggcgtaat cgttgaagtc
      721 aactgcgaaa ccgactttgt tgccaagacc gacaacttca aagattttgt cgccatggtt
      781 gccgagcagg tcgcgcagaa gaaccccgcg gacgtggatg ctctgcttgc gcaggattgc
      841 atttccaccc cgggtaccac gatctccggc atgctcaacg aagcggtgct taagattggc
      901 gaaaagatca gcattcgtcg tttcgcgcgc tatgaaggcg ttgtggactg ctatattcac
      961 cttggcggac gcatcggcgt tttgactgag atggatgcgc cggcgaacct gaaggacaac
     1021 gataccttca agacagttgc gcacgatatc tccatgcaga ttgccgcagc ccgtcccacc
     1081 tacctcaagc gggaagacgt ccccgccgag gaagttgagc acgaaaagga aatcaaccgc
     1141 gccatggcgc tcaacgaaga gaagccgaag cctgcggaag tcattgaaaa gatgctggtt
     1201 ggcagaatcg aaaagttcta caaggaagtc tgcctgcttg agcagctgtt cgtaaaggac
     1261 accagcatga acatcaccaa gctgctggag aacacttcca aggaactcgg cgcaaagatc
     1321 accgtcaagc ggttcgcccg tttcgaaatg ggcgagggcc tgcagaagcg tgaatgcaac
     1381 tttgccgaag agatcgccgc ggcgaccaag cactaaacaa ctgaataaga ggaacacgga
     1441 aacgtgttcc tttttttcgg ctcgccggag tataataaga atcaataaag accgtaaaga
     1501 aactgctggg cggaacaaac gaaaccgcct ggaaaggtgt gaaacaatgt atcatcgcgt
     1561 actcatcaaa gtcagcgggg aggcgctctc ctcggagcag ttcgtcatct gccgcgaaac
     1621 cgtgctcaaa acggcgaaca tgctcaaagc gattcataaa ttgggcgtgc agatcggcgt
     1681 ggtcgtcggc ggcggaaata tcatgcgcgg gcgcagcgcg gaaggcatgg agcgcaaccg
     1741 cgcggaccat atgggcatgc tcgcaacggc gatcaacgct ctcgccctgc aggacgcgct
     1801 ggagggcatc ggcgtgcctt gctccgttca gtccgcggtg gagatggagc gcttctgcga
     1861 ctcgttctct tcccgcgcgg caagaagcgc actggaggaa ggcaaggtcg tcatctttgc
     1921 ctgcggaagc ggctgcccgt ttttctcaac ggatacagcg gcggctctgc gcgcggcgga
     1981 aatcgacgcg gacgctctgc tgctggctaa aaatgtggac gccgtgtact cggcggatcc
     2041 gaagacggat cccaaagcgg tcaagtattc ccaaatcacc tatgaccgcg tagttgcgga
     2101 aaacctgcat gcaacagacc tgaccgcgat tacgctctgc aaggagcaga atattccgat
     2161 tcgcgttttt gcgctgagcc agattgagga agtttttcag gacgattcca tcggcactca
     2221 tattcttgct aacgcataaa aacgcatttt ttcgagtgcc cgcgcgtaaa aactacgccg
     2281 ggcactttgc gtttcttctt tttcagcaca gcgaccgtga cacaattaca cattctctct
     2341 tgaagtaaac aagtactcta tagtagaata aacaggaaca ggaggatgga accatggcag
     2401 atgtgatgat cgatagtacg aaggaaagaa tgaataaaac catcgccgtg ctcaaaagcg
     2461 agctgggcgg cctccgcgcg gggcgcgcga atccacagct tctggagcgc attttggtgg
     2521 actattatgg tacccctacg ccaatcccgc agcttggcaa catttccacg ccggaagcgc
     2581 gtatgctggt gattgcggtt tgggatcaaa aagcgattcc ggccattgaa aaagcgattc
     2641 aaaagtccga cttgggtatc aatccctcca acgacggtaa agtaatccgt ctgcttttcc
     2701 cggaactcaa cgaggagcgc agaaaggacc ttgtcaaagt cgtccataaa aagggcgaag
     2761 aatgcaaggt cgcaattcgt tcgatccgcc gggacgtgat ggaacagatc aagaaggact
     2821 gcaagagcag cacgattacc gaggacgatc agaaatacct cgaagagaaa attcagaagc
     2881 tcaccgacga catgatcaag gaaatcgaga cgattctcgc ggccaaggaa aaggagatcc
     2941 tcgcggtttg accagcgtac tcggctatcc gctggatcgg gcaatcgaag ttctgcagag
     3001 cgagggtttt tgcgttaaaa cggaagaagc gcgcagtaaa aaaggcgttt tggacggcaa
     3061 agaacagcgc gtgattcgcc agagcgaacc gattgaaaac aacgtaacac tggtctacgc
     3121 cttgtttaaa acggaaccga acgaaacgaa cgattaagta aggtgcaggg tgatctctgc
     3181 accttgctcc tgtgaaagag aacgatgcga acatacaccg acgaacaact gcagtctctc
     3241 ggcctgtccg ccgccgctct gcccagacac gttgctatca tcatggacgg caacggccgc
     3301 tgggcaaaga gccgcaggct cccgcggagc gcgggccacc gcgcgggcac cgaccggttg
     3361 cgcggcatca tccgcctctc ttccgacctc ggcatcgaag cgctgacgct ctatgcgttt
     3421 ttcacggaga actggaaacg cccggaggac gaggtaggaa cgctcatggg cttgctcgtt
     3481 gagtacttta ccaacgaaat cgacgaactg gatcagaacc gcgttcagat tcgcatcatc
     3541 ggagacacga caagactgcc gacgcgcgca aagaatgccg ttgaccgcgc catggcgcga
     3601 acgggttcca acgacggcct caagctcagc attgcgctca attacggcag ccgcgcggaa
     3661 atcttacttg cggcaaacaa ggccgcaaaa gcggcaaacg agcaggggct ggatgagata
     3721 ccccaggagc tgtttgagtc gtttctctnn nnnnnnnnaa agatttgccg gaagtcgatc
     3781 tggtgattcg caccagcggc gaacagcgaa tttccaactt cttgctttac cagatttcct
     3841 acgcggaatt cctgtttcca aaagagtact ggccggactt ttcggatgaa tgttatatta
     3901 acgcgctgcg ggacttttcc ggccgcgcta ggcgatacgg cggattgggg gaagaagcat
     3961 gaaaacaagg gtaattgtcg gcattctgct ggtgctcatg ctgattgccg tcctgttctt
     4021 cggagggttt cttctgctga gcgtgctttc gctgttctca ctcgcggcca tttacgaaat
     4081 gggcatgact ttccgcaaga aggggtacaa cccgatagtc ctgcccgcgt atattttcgc
     4141 ggtgtcttat agtttcgttt attactattt cggcctgctt gcgatgatca ttttctatat
     4201 ggcgaccgtg atggcgacga tgatttactc cctgtttaca aaggttcatt caacaaacga
     4261 catcatccct tcgctgttta tcatgaatta cccgactctg tttttgatgt gcatgatgct
     4321 cgtgtacttc aactttgacc gcacgaccgc gctagtcgcg gcgggcatgg cgtacgccgc
     4381 gccggaatgc gcggatacgt tcgcctattt cggcggcacg cttcttggca agcacaaact
     4441 ctgcccgact atcagcccaa agaagactgt ggagggcgca gcattcgccc tgcttggcgg
     4501 cattgccttc gggtttggca tgtattacct gcagcggctc tggggagcgg cgatcaatcc
     4561 gatcgttctg gtttctctcg gtcttggatg cgggctgctt tcgcagtttg gcgatttgtt
     4621 cgcttccacc atcaagcgct gggcgggagt taaggacttt tccagcgtgt ttcccggaca
     4681 cggcggaatc atagaccgca tcgacagcat tctgttctgc gcgccgctgg tgctctgcgt
     4741 gttcgttgtg ctgcgtcagc tagcggttct gtaatcaaac aaaaggtgtg agcggctcga
     4801 actaaatgtc gttaacgctc atcaggcaaa tgatatgact aagaagatta cggtgctggg
     4861 aagcaccggc tccatcggca ggcagacact ggacgtcatt tcttcgcagc ccgaagagat
     4921 gacgctcttt ggcttgtccg cccgcagcga tatcacgtcg ctgattgagc aggcaatcct
     4981 gtaccgtccg caaatcgttg caagcgaaac gccgttcgac ccttcgctgc tgcccgatgg
     5041 cacggaaacg atattcggcg atggcgcgag cacggttctg gcggccagaa cggaagcgga
     5101 cgttgtggtc aacggcgtaa gcgggtttgc ggcgctcgcg ccgctgctgg cagcgctcga
     5161 gcatggcaag cgcgtggctc ttgcgaataa ggaaagcatc gtctgcggca aacaacttgt
     5221 ggacgaaacg aaacgtcgct tcggcggcga aattattcca gttgacagcg agcagtccgc
     5281 tatcttccag tgcctgcaaa acgggcgcgc ggaggaagtg gaacgcctga ttctgaccgc
     5341 gtccggcggt ccgttctggc ggctggatcg accggctctg gagcaggtga cgcttgagca
     5401 ggcgctgtcg catcccacat ggaacatggg gaagaagatt accatcgact cggctacact
     5461 cttcaacaaa gggcttgagg tcattgaggc ggcgtatctc ttcgatctgc ccggcgaaca
     5521 gattgaggtg ctgattcatc cgcagtccat cgtacattcc atggttgcat accgcgacgg
     5581 cacctgcatg gccaatatga gcagaccgga tatgcgcctg ccgatacaat acgccatcac
     5641 gtaccccgcg cgtaacggtt ccgtctgtaa gccgctgctg ctgcaaaacg aaaaaccgct
     5701 tgagtttttc gagcccgatc tggcaaggtt tggtgcgctc cagctggcgt atcaaacgct
     5761 ggcaggtggt aagtcgtacc ccattgcgta taatggcgca aatgaggcgg cggtggaagc
     5821 attttgcggc ggcttgatcg ggtttatgga catcgaacgg gtggtggatt atactttaca
     5881 ggagcacagt cccgtcatag cggcaacgct ggaggagatt gtggaagcgg accgcgccgt
     5941 aagagttttg gcgaacagcc ggatcgaacg aatcgcgcgg gaaaggtagg aaacacggtt
     6001 tgtcgacggt tctttccatt tttgttgcac tgctgctttt atcgctgctg gtgatgattc
     6061 acgagctcgg ccattttctt gccgggcgcg ctttgggttt ctcgattctg gaatttgcca
     6121 tcggcatggg tccggtactc tggaaaacga ccagaaacgg gattcagtat tccatacgcg
     6181 cgctgcccat cggcggcatg tgccgcttta acggc
//
LOCUS       DAOMGY010000007         5778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_14062_length_5778_cov_3.338808, whole genome shotgun sequence.
ACCESSION   DAOMGY010000007 DAOMGY010000000
VERSION     DAOMGY010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14062_length_5778_cov_3.338808"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..278
                     /locus_tag="PLR57_00265"
     CDS             21..278
                     /locus_tag="PLR57_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HQQ39916.1"
                     /translation="MRLDKYLKVSRIIKRRTVANEAAGAGRVSINGKTAKPSSEVKEG
                     DTLDLLLGGRHLLVRVESLKESVRKEDAAAMYTVLSGVEDK"
     gene            275..1396
                     /gene="ispF"
                     /locus_tag="PLR57_00270"
     CDS             275..1396
                     /gene="ispF"
                     /locus_tag="PLR57_00270"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HQQ39917.1"
                     /translation="MSGVVGILLCAGGSTRMGFDKLLTPIAGKTAIERSLEALLRGGV
                     ERIVFAVSPSSKAFVASLAVPVPSDIVSGGATRAESVKHALEFLRLHQGAEIVAIHDA
                     ARCMVPPSAVRESIESAQQFGSGVVAAKTADTTMLIGSDGVPVPLERDRLVRMQTPQT
                     FRFDEILHAYEGDLTKATDDCSLYLAAGYVPRFVFVTGEAVNQKLTSAEDWQLALATY
                     AHFGTGFDTHRLVEGRKLILGGVEIPFEKGLLGHSDADVLTHAIIDALLGAASLGDIG
                     RHFPDSDPAYKGADSLELLKETARMIRLAGYSIGHIDATVIAQRPKLAPYWQSMRTRL
                     AEALEVERGAVSLKATTTEGMNDEGKGLCISASAVASLY"
     gene            complement(1486..2418)
                     /locus_tag="PLR57_00275"
     CDS             complement(1486..2418)
                     /locus_tag="PLR57_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQQ39918.1"
                     /translation="MGCSISFHPKTSLAAAEATPEPTSTQTPAPTATPVPTPFSIVWL
                     SDTQSITYYNYPHAMEDMGRWIAEQRVPENIRYVVQTGDMVDNGFVPKQWDIFDECYN
                     QFKDDIPYFAIAGNHDIGVKLQDYSAYLARPNVSSIPRRNSFERGRAVYATFEAGGTK
                     FLLLGAGWESEEMSVDWMNQVLKEHSDSVAILLFHSYIFMDGSYTNQGQMMFEQVVVP
                     NPNVRLVLCGHVSVSGARFEELDDNGDGVNDRKVTAMLYNYQDFMDNCGQLRLLTFDP
                     VARSIDVMTYSPYTGRYFWDDYFKTVEFTLEDAF"
     gene            complement(2589..3005)
                     /locus_tag="PLR57_00280"
     CDS             complement(2589..3005)
                     /locus_tag="PLR57_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39919.1"
                     /translation="MSVDWMNGILREHPNSVAILLFHGYIQTNGTYTVVGKKMFEQVV
                     RPNPNVKLVLSGHVLGTAVRFEDVDDNGDGAPDRRVTALMYNYQNADMDCGQLRLLTF
                     DPMARSLRVLTYSPYQKRYYRDFVYSSSDFLLENVF"
     gene            complement(3111..3560)
                     /locus_tag="PLR57_00285"
     CDS             complement(3111..3560)
                     /locus_tag="PLR57_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQQ39920.1"
                     /translation="MDPHANTVIREQVPGARISPPTATQGWMLPGHTPIPVIQSAPPT
                     DTPAPTPFSIVWLSDTQSMTYCNYTDALESMGRWIVDEKDERNILYVVQTGDAVENGF
                     SDWQWKNFDVCYDQFKDELPYIAIAGNHETGMKRHSYVGYLSRRNVS"
     gene            4100..5728
                     /locus_tag="PLR57_00290"
     CDS             4100..5728
                     /locus_tag="PLR57_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQQ39921.1"
                     /translation="MKRSAVLPIALACLAVLLCGGCSIIAPASSTSEPTVVPVASAEV
                     ITFADTSAPSQTPQLTPTPSAPKAAAATAVTGTTNQAAASAAIAAQMLRMEETSELVR
                     YWQTVQSDASIYFRQRKAVRAMLACTVGSGIEPVGREGIQFQPDPEVPLMEEGAELYK
                     GVDYNIRGSIYTSSPLTSVTAAMIPRAEGKSEEQTVTFDPAEQILDYSLDTKSDHSER
                     KGLGDLFDISALPAGRYTFTLSATTEANPTPVILYSVSCSIMNTRDMFLTQNKFDDNY
                     FEVYSFFKGDTSKFLFQYWIRDGRSISTDEEWRTKTLVESSLGRVNIDAVANFELANY
                     FLEHTYVSVTIVNNRTQKQTAGRVTLLKKLISKETTYVPRFQSNLQYVSHHTLGLAID
                     VNDDMYPNKNIETNHELVGTDVREHLVYNGIKTDDKGQQYYDFTYDGSYSARFERVPK
                     TLINYLLYELAFFRAGFQWGYYYESACDGMHFTLAEFDINRHMYSDVGLRKIYEYIED
                     ANIPTLESLVNGLPSPTPEGAAATPEATTPTPEE"
BASE COUNT         1333 a         1661 c         1566 g         1218 t
ORIGIN      
        1 cacgaaagga acattaacct atgcgtttgg ataaatacct taaagtctcc cgcatcatca
       61 agcggcgcac cgtggcaaac gaagccgcag gcgcggggcg cgtgagcatc aacggcaaaa
      121 cggccaagcc ctccagcgaa gtcaaggaag gcgacacgct cgaccttctg ctcggcggtc
      181 ggcatcttct ggtgcgcgtc gaaagcctga aggagagcgt ccgtaaggaa gacgcggcgg
      241 ccatgtatac cgtgctcagc ggagttgagg acaaatgagc ggggtcgtcg gcatcctgct
      301 ctgcgcgggc ggaagcacgc ggatggggtt cgacaagctc ctcaccccca tcgcgggcaa
      361 aaccgcaatt gaacgttcgc tcgaagcgct cctgcgcggc ggcgttgagc ggattgtatt
      421 cgccgtttca ccgtccagca aagcgtttgt agcgtcgctt gccgttccgg ttcccagcga
      481 tatcgtctcc ggcggggcaa cgcgcgcgga gtcggtcaaa cacgcgctgg agtttctgcg
      541 ccttcatcag ggcgcggaga tcgtcgccat acacgacgcg gcgcgctgca tggttccgcc
      601 gtcggccgtc cgcgagagca tcgaaagcgc gcagcagttc ggcagcggcg tcgttgccgc
      661 caaaaccgcg gacaccacca tgctcatcgg cagcgacggc gtacccgttc cgctggagcg
      721 ggacaggctc gtgaggatgc agacgccgca gacattccgc tttgacgaga ttctgcacgc
      781 gtacgaaggc gacttaacaa aagcgacgga cgactgctcg ctctatcttg cggcggggta
      841 tgttccgcgc tttgtgtttg taaccggaga agccgtcaac caaaagctca cctcggcgga
      901 ggactggcag ctcgcgctgg caacctatgc gcactttggc accgggttcg atacccaccg
      961 gctcgtcgaa gggcgaaagc tgattctcgg cggcgtggag attccgtttg aaaaaggttt
     1021 gctcgggcat tccgacgcag acgtgctgac gcacgccatc atcgacgcgc ttttaggcgc
     1081 ggcgtcgctc ggcgacatcg gcaggcattt tccggactcg gatcccgcct acaagggcgc
     1141 ggacagtctg gaactgctca aggaaaccgc gcgcatgatc cgccttgccg ggtacagcat
     1201 cgggcatatc gacgcaacgg tgatcgcaca gcgcccgaag cttgcgccgt actggcaaag
     1261 tatgcgcacg cgccttgcgg aagcgctaga ggttgagcgc ggcgcagtct cgctgaaagc
     1321 gaccacgacg gaaggcatga acgacgaagg aaagggcctc tgcatctccg ccagcgcggt
     1381 tgcctctcta tactgaatca gccaaacagc ctaaagcaca ataaacaaag agcgtcccga
     1441 acggaacgct ctttttgcat tcttcacatg ctgttaaacc gtcacttaaa acgcgtcttc
     1501 cagcgtaaac tccaccgtct taaaatagtc gtcccagaaa taccgaccgg tatacggcga
     1561 ataggtcatt acgtcgatcg agcgcgcaac gggatcgaac gtcagtaagc gcagctgccc
     1621 gcagttgtcc ataaagtcct gatagttata caacatggcg gtgaccttgc ggtcgttcac
     1681 gccgtcgccg ttgtcgtcca gctcctcaaa gcgcgcgccg gaaacgctga cgtgcccgca
     1741 aagcacgagc cgcacgttcg ggttgggcac aacaacctgc tcgaacatca tctgcccctg
     1801 atttgtgtag gaaccgtcca taaaaatata cgagtggaac agcagaatcg caacagaatc
     1861 gctgtgctcc ttcagcacct gattcatcca gtctacggac atttcttcgg attcccagcc
     1921 agcgccgagc agcaaaaact tggttccgcc cgcttcgaat gtcgcataga ccgcgcggcc
     1981 gcgctcaaag gagttgcgcc gcgggatact gctgacgttc ggacgcgcaa gatacgccga
     2041 atagtcctgc agctttacgc cgatatcatg gttgcccgcg atggcgaaat aggggatatc
     2101 gtccttaaac tgattgtagc actcgtcgaa gatgtcccat tgtttcggga cgaagccgtt
     2161 atcgaccatg tcgcccgtct gcacgacgta acgaatgttt tcgggcactc tctgctcggc
     2221 tatccagcgg cccatgtctt ccatggcgtg cggataattg taataggtta tgctttgcgt
     2281 atcggaaagc cagacgatgg aaaacggcgt cggcactggc gttgccgtcg gtgccggtgt
     2341 ttgcgtgctg gttggttcag gcgttgcctc ggcggcagcg agcgaagttt tggggtgaaa
     2401 cgaaatggaa caccccataa aaaaggcggc cgcaacacag ccgcaaaaaa gaagtaacac
     2461 gtgcaagcgt tttttcatac cacattctag cacaactgtt ttcccgcaag caatcgcgcg
     2521 gacgtcattt ttggattcca aatgtgattt ctatgtgaca aagaagtcgc cgaaaaatcg
     2581 ctttccgatt agaacacgtt ttccagaagg aaatcgctgc tggagtacac aaaatcacga
     2641 taatatcgtt tctgataagg cgaataggtc aagacgcgaa gcgaacgcgc catcgggtcg
     2701 aacgttaaga gccgcagctg tccgcagtcc atatccgcgt tctgatagtt atacatcagc
     2761 gcggtcacgc ggcgatcggg cgctccgtcg ccgttgtcat ccacgtcctc aaagcgaacg
     2821 gcggtgccca gcacatgccc gctgagcacg agcttgacgt tcgggttggg tcttaccacc
     2881 tgttcaaaca tctttttgcc gacgacggta taggtgccgt tggtttggat gtaaccatgg
     2941 aacagcaaaa tcgcaacgga atttggatgc tcgcgcagga ttccattcat ccagtctacg
     3001 gacatcagct cggaatccca accagcgccg agaatgagaa aatcgatacc gcccgcacgc
     3061 agggtagcgt aagcggcgcg cccgcgctca aaggagtacc ggcgcggtat ttagctgaca
     3121 ttcctgcgcg agagatatcc aacataggaa tgccgcttca tgccggtttc gtgattaccc
     3181 gcaatggcga tatacggcag ttcatctttg aactgatcgt aacagacgtc gaaattcttc
     3241 cactgccagt cggaaaagcc attctccacc gcgtccccgg tctgcacgac gtagagaata
     3301 ttccgctcgt ccttttcgtc cacgatccag cgccccatgc tctccagcgc gtcggtatag
     3361 ttgcagtagg tcatgctctg cgtgtccgaa agccaaacaa tggaaaacgg cgtcggcgcg
     3421 ggcgtgtccg taggaggcgc gctctgtatc acggggatcg gcgtatgccc ggggagcatc
     3481 caaccctgcg ttgccgtcgg aggagagatt cttgcgcccg gcacttgttc gcgtatcacc
     3541 gtatttgcgt gcggatccat tccctgctgc ccgccatcca gaacggcggc cccggcagga
     3601 cgtaatttcc cgcatcccgc gccaaggaaa agaaggccaa cggcagtcag gcttgtcaca
     3661 acaaccgcta ttgaacgtaa cacttttttt atacaagatg tggtaacatg catctgattt
     3721 gtaccgtaaa ccctgcctct gtaaaatata tgaaaaaccg gatatttaac gctcagagcg
     3781 tgttttttca tgattctttt tgtttcatgg ggtgccaaaa atgtaacaaa ataaaaccac
     3841 gaatgtaaca tagtgcacgg ctgacggctg ccgctgtgca gttttttaga atcggtatgt
     3901 gaaaatggct ggaaaagcga tgtgctggat gaaatcggaa cgggttccgc atggcggact
     3961 tttctttctg tccgatctcg tatataataa cctttactga attgtttttg caatggtgaa
     4021 atcgtgcgaa atcgtacgaa tttaatattt tattgtgccc cttgcccgga atgtgttccg
     4081 atttcaccct ggaggaacta tgaaacgatc cgctgtcctt cctatcgcgc ttgcctgcct
     4141 ggccgttctt ttgtgcggcg ggtgttccat tatcgctccc gcgtccagca cgtcggagcc
     4201 tacggttgtt cccgttgctt ccgccgaggt cattacgttt gccgatacca gcgcgccttc
     4261 ccaaacaccg cagctgacgc cgactcccag cgcgccgaag gccgccgctg caactgccgt
     4321 caccggcacc accaatcagg ccgccgcttc cgccgccatt gccgcgcaga tgctgcgcat
     4381 ggaagaaact tccgagctcg tccgctactg gcagaccgtt cagtccgacg cttcgattta
     4441 cttccggcag cgtaaggcgg ttcgcgccat gctcgcctgc acggtcggca gcggcattga
     4501 gcccgtggga cgggaaggca ttcaattcca gcccgatccg gaagttccgc tcatggaaga
     4561 aggcgcggaa ctctataagg gcgtggacta caacattcgc ggctccatct ataccagcag
     4621 tccgctcacc agcgtaaccg ccgcaatgat tccgcgcgcg gagggcaaga gcgaggagca
     4681 aaccgtaaca ttcgaccccg ccgagcagat tcttgattac tcactggata caaaatccga
     4741 ccacagcgag cgaaagggtc tgggcgattt gtttgatatc agcgccctgc ccgcgggacg
     4801 gtataccttt acgctgtccg ccaccaccga agccaacccg acgccggtta ttctctacag
     4861 cgtttcgtgt tcaatcatga atacaaggga catgttctta acgcagaaca agttcgacga
     4921 taactatttt gaggtctatt ctttcttcaa gggcgatacc agcaagtttt tattccagta
     4981 ctggattcgc gacggcagaa gcatatcgac cgatgaggaa tggcgtacaa aaacgctggt
     5041 ggagagcagc cttgggcgcg tcaatatcga cgcggttgcg aactttgagc tggcaaacta
     5101 tttcctcgag catacgtatg tcagcgtaac cattgtcaac aacaggactc agaagcagac
     5161 cgccggtcgc gtaacgctgc tcaagaagct gatttcgaaa gaaacgacgt atgtgccgcg
     5221 ctttcagtcc aacctgcagt atgtcagcca tcatacgctc ggcctcgcca tcgacgtgaa
     5281 cgacgacatg taccccaaca agaacattga aacaaaccac gagctggtcg gcaccgacgt
     5341 tcgagagcat ctcgtctata acggcatcaa gacggacgat aaaggtcagc agtactacga
     5401 cttcacatac gacggctcct attccgcccg ctttgagcgc gtacccaaga cgcttatcaa
     5461 ctacctgctc tacgagctgg cgttcttccg cgccggattc cagtgggggt attattacga
     5521 gagcgcctgc gacggcatgc actttacgct cgcggagttc gacatcaacc gccacatgta
     5581 ttcggacgta ggtttacgaa agatctatga gtacattgag gatgccaaca tccctaccct
     5641 tgagtcgctc gtaaacggcc tgccttcccc cacgcccgaa ggcgctgcag caacgccgga
     5701 agccacaacg cctacgccgg aagagtaacg gaggtcttct ttttctcgag agaaaaagaa
     5761 gcaaaaagag cttccttt
//
LOCUS       DAOMGY010000008         5662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_14437_length_5662_cov_3.319957, whole genome shotgun sequence.
ACCESSION   DAOMGY010000008 DAOMGY010000000
VERSION     DAOMGY010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14437_length_5662_cov_3.319957"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLR57_00295"
     CDS             <1..514
                     /locus_tag="PLR57_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ39922.1"
                     /translation="LQSLRQNVGIVQQDVFLFASSVLENIRYGRIDATDAEVVEAAKR
                     AHIHDEILLFPNGYETEVGERGIMLSGGQKQRVSIARLFLKNPRVLILDEATSALDTM
                     TEHVIQHAFEELSEGRTTLVIAHRLSTVKHADEIVVLDEKGVRERGTHEELLKRNGLY
                     AQLYHATMIE"
     gene            592..1152
                     /locus_tag="PLR57_00300"
     CDS             592..1152
                     /locus_tag="PLR57_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HQQ39923.1"
                     /translation="MKIKKRTLAIVVIILLVLAALWFLRGQTTDGLAQVSPQATSSLV
                     ELAGEDASGGEQNGALPQQPASADETAPGEFGEYTTKDDIAAYLIAYGRLPQNFITKS
                     EARALGWSGGGLDDYDYGKCIGGDEFGNNEGLLPEQAGRVYYECDVDTLHQDDRGAKR
                     IVFSSDGLIFYTDDHYESFTQLNPEQ"
     gene            1174..1467
                     /locus_tag="PLR57_00305"
     CDS             1174..1467
                     /locus_tag="PLR57_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HQQ39924.1"
                     /translation="MQKSRVVVLNGKRMRTRERAHTHLARRFRLPSWYGRNLDALCDC
                     LGEINVPTVILLRHARRLRESLGDYGAKLISVLAAMTGENPNLSLVVHQKYFK"
     gene            1766..2497
                     /locus_tag="PLR57_00310"
     CDS             1766..2497
                     /locus_tag="PLR57_00310"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQQ39925.1"
                     /translation="MLRLQKYLAEAGVASRRGGEELINAGRVSVNGVVAQIGMSVDPD
                     RDMVTLDGKPVGRKEEIITVALYKPKGVVSTSEDPEGRKTVQEFVRDIPVRLYNVGRL
                     DLNSEGLLLMTNDGELAYRMTHPKFAVEKTYYAVCDGKLTASEIALLVNGVQLEDGKT
                     APTKVEHVRPTKAGDTSFSITIHEGRNRQIRRMLETVGHRTLRLKRERFGPITIGQMK
                     PGDVRRLTEREVAALKKDLGMRSPV"
     gene            2686..2982
                     /locus_tag="PLR57_00315"
     CDS             2686..2982
                     /locus_tag="PLR57_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39926.1"
                     /translation="MDFLHLTLLIGGVIGVALVAAVLGSNAIQKDILQELRAGKGFFA
                     AGAAAELPQYAASAALEAPIPNRNEVLAIAAPCIAEVMGKDVTGLRIVSIKKVG"
     gene            3052..3423
                     /locus_tag="PLR57_00320"
     CDS             3052..3423
                     /locus_tag="PLR57_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQQ39927.1"
                     /translation="MRNFLVNINGTSYEVSVEEIDGKAAPAVKPVAPAAAPAPAAAPA
                     APKPAASAGGTPVLCPMPGTIVAVNVKEGDAVKSGQALFSLEAMKMESDIPAPVDGIV
                     RGIAVRKGDATEAGGLLCSIE"
     gene            3584..5017
                     /locus_tag="PLR57_00325"
     CDS             3584..5017
                     /locus_tag="PLR57_00325"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HQQ39928.1"
                     /translation="MSKLFITDTILRDAHQSQAATRMRIEDMLPACEVLDSIGYWSLE
                     VWGGATFDACLRFLNEDPWDRLRKLRKALPNTKLQMLFRGQNILGYKHYADDVVEQFC
                     KKAIENGIDVIRIFDALNDIRNLESAIRFTKKYGGICEPAISYTVSPVHNEDYFVAMA
                     KELVQMGADTICIKDMANLLLPYDAFQLITRLKKEISVPIHLHTHNTTGTGDMVYLMA
                     AQAGVDIVDTALSPFGNGTAQPATEPLVATLAGTERDTGLDLRKLSDAAAFFRPVKDR
                     MLADGTVSVKALGVDTNTLLYQVPGGMLSNLISQLKEAGKSDRYYDVLEEVPRVRKDF
                     GYPPLVTPTSQIVGTQAVLNIINGERYKVFTKESKALLHGEYGMLPGPVNEEVRKKAI
                     GDEEVITCRPADLLKPELESYREEIKDHIRQEEDVLSYALFPQVALKFFEAREAAERK
                     AAEPAPEPKQEAQETGVRTLYVEDLGA"
     gene            5018..5596
                     /locus_tag="PLR57_00330"
     CDS             5018..5596
                     /locus_tag="PLR57_00330"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQQ39929.1"
                     /translation="MRHLPNILSGLRLVLVGVFVWLFRAGRYLPALSIFVFAFFTDVL
                     DGQLARANGWVTNLGKLLDPLADKLMTLAALVCIYLGKQKSVYLVLFLLMAAKEALMV
                     AGGIFMARRSVVAMADWPGKLSTGLFAVGVMLSLLSFLSLQVEPWNLYVLGAAVAVSY
                     FALIFYASTQLPKAFSQKKAEAGDEAGKLNGK"
BASE COUNT         1312 a         1576 c         1666 g         1108 t
ORIGIN      
        1 cttgcagtcg ctccgccaga acgttggcat cgttcagcag gacgtgtttc tctttgccag
       61 cagcgtgctc gaaaacattc gctacggccg cattgacgcg accgatgcgg aggtggtaga
      121 ggccgcaaag cgcgcgcata tccacgacga aatactgctc ttccccaacg ggtacgaaac
      181 cgaggtgggc gagcgcggca tcatgctctc cggcgggcag aaacagcgcg tttccattgc
      241 gcggctgttt ttgaagaacc cgcgcgttct gattctggac gaagccacca gcgcgctgga
      301 cacgatgacc gagcatgtga ttcagcatgc gtttgaagag ctttcagaag ggcgcaccac
      361 gctggtgatt gcgcaccggc tctccaccgt aaagcatgcg gatgagatcg tcgttctgga
      421 cgaaaagggc gttcgcgaac gcggaacgca cgaagaactg ctcaaacgga acgggctgta
      481 cgcgcaactg tatcacgcga cgatgattga ataaaccggt cggcaaagcc gtttggggat
      541 aaatccggcg gaacaaacaa gatcatgagt atagagagga agagcggcgg aatgaaaatc
      601 aaaaaacgta cgctggccat cgtcgtcatc atcctgctcg tgctcgccgc gctgtggttt
      661 ctgcgcgggc agacgacgga cggacttgcg caagtctccc cgcaggcaac gagttcgctg
      721 gttgaactcg ccggcgagga cgcttccggc ggcgaacaaa acggcgctct gccgcagcag
      781 cccgcctcag cggacgaaac cgcaccgggc gagtttggcg aatacacgac gaaggacgat
      841 atcgccgcgt atttgattgc gtacggcaga ctgccgcaaa actttattac caagtccgaa
      901 gcgcgcgcgc tcggctggag cggcggcggg ctggacgact acgattacgg caagtgcatc
      961 ggcggagacg aattcggcaa caacgaggga ctgctgccgg agcaagccgg acgcgtgtat
     1021 tacgagtgcg acgtggatac cctgcatcag gacgaccgcg gcgcaaagcg aatcgtcttt
     1081 tccagcgacg gcctgatttt ttatacggac gatcattacg aatcgtttac gcagcttaat
     1141 ccggagcagt aaacagagat gggaggggtg cgcttgcaga aaagccgggt tgtcgtgctc
     1201 aacggaaaac gaatgcgtac gcgggaacgc gcgcatacgc atcttgcgcg ccgcttccgt
     1261 ctgccaagtt ggtacggcag aaatctggac gcgctgtgcg attgccttgg agagatcaac
     1321 gttccaaccg tgattctgct gcggcacgcg cggcgtttga gggaatctct gggcgactac
     1381 ggcgcaaagc tgatatccgt gcttgccgcc atgacggggg agaatcccaa cctttcgctg
     1441 gtcgtgcacc agaagtattt caagtaatat gtttcaggat taagcagaca agacaaaacc
     1501 gatgaaccta tgccggggca gacgccccgg ttttttcttt tttacgcaaa aaaacgaaaa
     1561 tatattttat aaatcgattg gcgacggcgg catcttcctg tatactatat ttggtgaaga
     1621 ttggcatgct cttgtcagcg gaacagtaaa tgcgctgttt gcggaaccgg agcaggcagg
     1681 ttcccgccgg gaggcggcaa ggccggagtt catccggaac aacagtcccg ccgggaggcg
     1741 gcatttttag aaaagagggt caaccatgct gcgactgcag aaataccttg cggaagcggg
     1801 cgtcgcttcc aggcgcggag gcgaagaact catcaatgcg gggcgcgtaa gcgtcaacgg
     1861 cgtagtggcg cagatcggca tgagcgtcga tcccgaccgc gatatggtta cgctggacgg
     1921 caaacccgtc gggcgaaaag aagagatcat cacggttgcg ctctacaaac ccaaaggcgt
     1981 ggtcagcacg tcggaagacc cggaagggcg aaagaccgtt caggaattcg tgcgggatat
     2041 tccggtaagg ctctacaacg taggcaggct cgatctcaac tccgaaggcc tgctgctcat
     2101 gaccaacgac ggcgaactgg cctaccgcat gacacatccc aagtttgcgg tggaaaagac
     2161 ttattacgcc gtttgcgacg gaaagctcac cgcttccgaa attgccttgc tggtcaacgg
     2221 cgtgcagctt gaagacggca agaccgctcc cacgaaggtg gagcatgtcc ggcccacgaa
     2281 agcgggggac acttcctttt ctataacgat tcacgaaggc agaaaccgcc agatcaggcg
     2341 gatgctggaa acggtcgggc accgcaccct gcgcctcaag cgtgagcggt tcgggccgat
     2401 tacgataggg cagatgaaac cgggcgacgt gcgccggctc accgagcggg aagtagccgc
     2461 gctgaagaaa gacctcggta tgcgttcgcc cgtttaaacc gctgtaagaa ctggaaaata
     2521 ttttataagt tccaggcagg aacggcagga gagctgtcga attccgaaaa aactttttct
     2581 atttcaggca ggaacgacag gagtactgtc gaatatatag aacatgggat aggatgatga
     2641 tatctagtat ccctgtttga gtaattattc tctggaggta taaatatgga cttcttacat
     2701 ctcaccttgc tgatcggcgg agtcattggc gttgcgcttg tggccgccgt tctcggctcc
     2761 aacgcgattc aaaaggatat ccttcaggag cttcgcgcgg gcaagggctt tttcgccgca
     2821 ggcgcggcag ccgaactgcc gcagtacgca gcttcagcgg cactggaagc gccaattccc
     2881 aaccgcaacg aagttctcgc catcgccgcg ccctgcattg cggaagtcat gggcaaggac
     2941 gtaaccggac ttcgtatcgt ctccatcaaa aaagtcggct gaaacgagcg ttcagcacaa
     3001 gttcaggcaa aaacattaaa tttcacatag aggaaaatat cggaggtatt tatgcgtaat
     3061 ttcttagtca acatcaacgg aaccagctat gaagtcagcg tcgaagaaat cgacggaaaa
     3121 gcagcacccg cagtcaagcc cgttgcgccc gcagcagcac ccgctccggc agccgctccc
     3181 gcagcgccca agcccgccgc ttccgcaggc ggaaccccgg ttctctgccc gatgcccggc
     3241 accatcgttg ccgtcaacgt caaggaaggc gacgcagtca aaagcggtca ggcactcttc
     3301 tcgctggaag ccatgaaaat ggagagcgac attcccgcgc ccgtcgacgg catcgttcgc
     3361 ggcatcgcag tccgcaaagg cgacgccacc gaagccggcg gcctgctttg ctccatcgag
     3421 taaggcggaa acgtcaaagc aagcgtacct tccggcaacg aacggttccg ccgcgctcaa
     3481 ttgcgcgccg ggattccgtt tgccgggata tttgacgata gttaccgtga cgacgcgcgc
     3541 gcagatgatt tggaagcgcc gcgccgtgta aaaggagaaa agcatgagta aactcttcat
     3601 tacagatacc atcctccgcg atgcgcatca gtcccaggcg gcgacgcgca tgcgcattga
     3661 ggacatgctt ccggcctgcg aagtgctcga ttccatcggg tactggtcgc tggaagtctg
     3721 gggcggcgca acgtttgacg cctgcctgcg ttttctcaac gaagatccct gggatcgtct
     3781 gagaaagctc cgcaaagcct tgcccaacac caagctgcag atgctcttcc gcggccagaa
     3841 catcctcggc tacaagcatt atgccgacga cgtggtcgaa cagttctgca aaaaggcgat
     3901 tgaaaacggc atcgacgtta tccgtatttt tgacgcgctc aacgatatcc gcaacctcga
     3961 atcggcgatt cgctttacta agaagtacgg cggaatctgc gagccggcca tcagctatac
     4021 ggtctctccc gttcacaacg aggactactt cgtggctatg gccaaggaac tcgtacagat
     4081 gggcgcggat acgatctgca tcaaggacat ggcgaacctg cttctgccgt acgacgcgtt
     4141 ccagctcatt acgcgcctta agaaggaaat cagcgttccg atccacctgc atacccacaa
     4201 caccaccggc acgggcgaca tggtttacct catggccgcg caggcgggcg tggacatcgt
     4261 ggacaccgcg ctttctccgt ttggcaacgg caccgcgcag cccgcgaccg agcctttggt
     4321 tgcaaccctt gcaggtaccg agcgcgacac tgggctcgac cttcgtaaac tctcggacgc
     4381 ggcggcgttc ttccgccccg tcaaggatcg tatgctggcg gacggcacgg tttctgtgaa
     4441 agccctcggc gtagatacga acaccctgct ctatcaggtt cccggcggca tgctcagtaa
     4501 cctgatctcc cagcttaagg aagcgggaaa gagcgaccgg tattacgacg tgctggaaga
     4561 agttccgcgc gtacgcaagg acttcgggta tccgccgctt gttacgccga caagccagat
     4621 cgttggcaca caggccgtgc tgaacatcat caacggcgag cgctataagg tattcaccaa
     4681 ggaatccaag gcgctgctgc acggcgaata cggcatgctg cccggccccg tgaacgagga
     4741 agtccgtaaa aaggctatcg gcgatgaaga agtcatcacc tgccgcccgg cggatctgct
     4801 caaaccggag ctggaaagct accgcgaaga gatcaaggat cacatccgtc aggaagagga
     4861 cgtgctgagc tacgcgctgt tcccgcaggt tgcgctcaag ttctttgaag cgcgcgaagc
     4921 ggcggagcgc aaagccgccg aacccgcgcc cgaacccaaa caggaagcgc aggagaccgg
     4981 cgtacgcacg ctctacgttg aagatttggg ggcgtaaatg cgacatttgc cgaacatcct
     5041 ttccggtttg cggttggtac tggtcggcgt gttcgtctgg ctcttcaggg caggcaggta
     5101 tttgcctgcc cttagcatat tcgtgttcgc gttctttacg gatgtgctgg acggccagct
     5161 tgcgcgcgca aacggctggg tcaccaacct tggcaagtta cttgacccgc tggcggacaa
     5221 actcatgacg ctggcggcgc tggtctgtat ttatctcggc aaacaaaaat ccgtttacct
     5281 cgttctgttt ttactcatgg ccgccaagga agcgctcatg gtggcgggcg gaatcttcat
     5341 ggcgcggcgg agcgtcgtcg ccatggcgga ctggccgggc aagctctcaa cggggctgtt
     5401 tgcggtcggg gtcatgctct cgctgctgtc gtttctttca cttcaggtcg agccctggaa
     5461 cctctacgtg ctcggtgcgg cggtggcggt ttcgtatttt gcgctgatct tttatgcttc
     5521 gacccaactg ccaaaagcgt tctcacaaaa gaaggcggaa gctggcgatg aggctggaaa
     5581 gctgaacggg aaataacaca gagagggttt cagcgccgga aacggcgcga aaatgttctt
     5641 gcattcgctg taagtggatg ct
//
LOCUS       DAOMGY010000009         5637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_14528_length_5637_cov_3.425833, whole genome shotgun sequence.
ACCESSION   DAOMGY010000009 DAOMGY010000000
VERSION     DAOMGY010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14528_length_5637_cov_3.425833"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..2298)
                     /locus_tag="PLR57_00335"
     CDS             complement(343..2298)
                     /locus_tag="PLR57_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39930.1"
                     /translation="MKRKANLPLLAWVCCLAVMFACMAVANAYQTDFGKIEVTRGAFM
                     TDDGNVITYKLYRPVTATAETPAPAVLLMHGYQNDKDTSSSYALELARRGIVALSIDE
                     YGHGGTSIGMLARGYTYHKLPNWDKSVSGPERFLLMMNFNTSDFFTNLADVPGDTLGD
                     TSMGGRTMYAYLSTLPFVDASNMGVTGHSMGTWSSWSVAATNPSVKAVVLQCGELFPQ
                     SYYDNEAIHFNNVLLIQSRYDEFTSFLDYTKAVPDDLVNTELRYKEFAGQDGLIEWNT
                     TYGSFADGTARRMELITNANHRLVTINNHAIATTLDWFETAFQMDSTIASTSQTALIK
                     ETFLFLGTLAALASVLPLLLLLLKTKFFAPCAQPMPDRSETLLPRKKWWGAALTAILI
                     SGLSYPFITQLGHGLVPLPENIFRMTIGDGVITWFVFLALVAFFMLRHWFKRGAGKKA
                     GETLYDLGLASKETPNKLPWGIIGKSALLALILAGSIYIYVTVFTQIYALDFRFVWPL
                     FKPFTLQRFWQFLLYVPFYLVFFCINGGAKLYGQLRQKELKSPAATQIVWWLKGSLVM
                     IGGLLLVCLIEYIPYFSGIGPGMDILFTSTFGGPFISFLIVIIPQFILLFFLSTYAFR
                     KTGRVYVGSVLLAILGAWAVTAGSSML"
     gene            complement(2461..3219)
                     /locus_tag="PLR57_00340"
     CDS             complement(2461..3219)
                     /locus_tag="PLR57_00340"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HQQ39931.1"
                     /translation="MKHTAIGTFTPESIREQVTRELQRRIFSGELAVGERLPPERDLA
                     KLLGVSRSLVNLAVLDLESMGFVRIVPRQGTFVADYKTQSTPQMLLSLMTHGAEDANA
                     ELFASMMETRRLLETECARLVTEKASDDEIQKLSILLDEMRNTREPAQFSVANFRFHR
                     AMLAASGNIVYAMIFQSFEQVVLYYVTSYFTTPERISLSIEQHEKLMEALKTRNAPLA
                     KTEIERIMEEGISNLTRMFSPKVIQAKTPPKFRA"
     gene            3531..3812
                     /locus_tag="PLR57_00345"
     CDS             3531..3812
                     /locus_tag="PLR57_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39932.1"
                     /translation="MTDRKRLIVTILILVAAAGAFVLGLIILPDTLVMQVTANGQPGT
                     QMPKLIGLLIPLALSTVFSILYCKRDGAKNLIVALVGIGMSALTFAFNL"
     gene            4343..5395
                     /locus_tag="PLR57_00350"
     CDS             4343..5395
                     /locus_tag="PLR57_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ39933.1"
                     /translation="MKNTLKKITASVLSILLVLALFAGCTAAPAPEATEAPAATEAAV
                     EAIATEAPVEETFTVGVLQFGSHPSLDNCYNGIAEALSADPRITIDLQNGNFDSPTCD
                     TIAKNMAAKQYDLIIAIATPAAISAYAATANTDIPVIFCAVSDPVAAGLVDDMNAPGG
                     NCTGTSDVLDLSAQLELIRAFQPDAVNLGVLYTTSEANSVSQLKTLNELAPQYGFTVV
                     SQGVQSAADIPQAAATLCAQVDCLTNFTDNNVVNNLAILLEQANAAGVPVYGSEETQV
                     TAGCLASVSLDYLALGRVTGQMALDVLGGADASTMAVKTITDAVPVVNSDVLAAFGYT
                     LPEAYANATLVTTTAE"
BASE COUNT         1319 a         1543 c         1618 g         1157 t
ORIGIN      
        1 tggtggaaat tcggatatcc gcgctgaagc ggcggacagc ggaacaatcg agcatgcgca
       61 ggaaatctcc tttcggtgaa acgggtgaac gatgcgctga aaacgactga aaagccgcat
      121 aaactctgat gaaatgaatg tttcgataat cctatcatat cgacaggcag tccaaagcgc
      181 aatgcgctat ttgccgtatg gttgcgcaat aagcaacgag tggtacagta tttggcgcgt
      241 tttgcgtcca gcgcaacagg actgccgcat acgacaaaga gagccggtct aaaagccggc
      301 tctctgaagc tcaaatcgta aatgttgttt gaaaggaatt gcttacagca tgctcgaacc
      361 tgccgtgacc gcccatgctc cgaggatggc caagaggacg gagccgacgt agacgcgccc
      421 ggttttgcgg aacgcgtagg tcgagaggaa gaagagcagg atgaactgcg ggatgatcac
      481 gattagaaac gagatgaacg gcccgccgaa cgtggacgta aacaggatat ccattcccgg
      541 cccgattccc gagaagtacg ggatgtactc aatcaggcaa acgagcagca gcccaccgat
      601 catgacgagg cttcccttga gccaccagac gatctgggtc gccgcggggg atttcagttc
      661 cttttgccgc agctgtccgt agagctttgc gccgccgttg atgcagaaga acacgagata
      721 gaacggcaca tagagcagga actgccagaa gcgctgcagc gtaaacggtt tgaacaacgg
      781 ccagacaaag cggaagtcca gcgcgtagat ctgcgtaaag accgtgacat agatataaat
      841 tgaacccgca aggatcagcg ccagcagagc ggatttacca atgatgcccc aagggagttt
      901 atttggcgtt tctttgctgg caaggccgag gtcgtagagc gtttctcccg cctttttgcc
      961 cgcgccgcgc ttgaaccagt gccgcagcat gaagaacgca acgagagcca ggaacacaaa
     1021 ccaggtaatc acgccgtcgc caatggtcat gcggaagatg ttctccggca gggggacaag
     1081 cccgtggccg agctgcgtaa taaacgggta cgacagcccg ctgataagaa tggccgtcag
     1141 cgccgcgccc caccactttt tccggggcag cagcgtttcc gaacgatctg gcatgggctg
     1201 cgcgcaaggc gcaaagaact tagttttcag caacagcagc agcagcggca gcacggaagc
     1261 cagcgcggca agcgtaccga ggaacaggaa cgtctccttg atcagcgcgg tctggctggt
     1321 ggaggcaata gtggaatcca tctggaacgc ggtctcaaac cagtccagag tggttgcgat
     1381 ggcgtggttg ttgatggtga ccagccggtg gttggcgttg gtaatcagct ccatccgccg
     1441 cgcggtgccg tcggcaaagg agccgtaggt ggtgttccat tcaatcaggc cgtcctgacc
     1501 cgcaaactcc ttgtacctga gttccgtgtt gaccagatcg tccggcacgg ccttggtata
     1561 gtcgagaaaa ctcgtgaatt catcgtaacg tgactggatg agaagcacgt tgttgaagtg
     1621 aatcgcctcg ttgtcgtaat agctctgcgg gaaaagctcg ccgcactgca gaacgaccgc
     1681 cttgacgctt ggattggtgg cggcaacgct ccagctcgac caggtgccca tggaatgccc
     1741 cgttacgccc atgttgctcg catccacgaa cggcagggtg gagaggtagg cgtacatcgt
     1801 acgtccgccc atggaggtgt ctcccagcgt gtcgcccgga acgtccgcaa gatttgtaaa
     1861 gaagtccgaa gtgttgaagt tcatcatcaa gaggaaccgc tccggcccgc tgacggattt
     1921 gtcccagttg ggcagcttgt gatacgtgta gccgcgcgcg agcatgccga tgcttgttcc
     1981 gccgtggccg tattcgtcaa tcgagagcgc aacgatgccg cgccgcgcga gctccagcgc
     2041 gtaggaactt gaggtatcct tgtcgttctg gtagccgtgc ataagcagca ccgcgggcgc
     2101 gggcgtttcc gccgtcgccg taaccgggcg gtagagcttg taggtaatga cgttgccgtc
     2161 gtcggtcatg aacgccccgc gcgtgacttc gattttgccg aagtcggttt ggtaggcgtt
     2221 ggcaaccgcc atgcaggcaa acatgaccgc aagacagcag acccaggcga gtaagggcag
     2281 attggctttg cgcttcataa aagaagttcc tcctatatgc aattcgaaat atcatgcata
     2341 attgaacaaa aggatggttt ctgatcatcg ttcaaacaaa gaagaacggc aaacaatccg
     2401 aaccggaaag gagatcggga cagtggacaa gttaacaaaa agggaagaga aaaaccttgt
     2461 tcaggcgcgg aattttggcg gggtcttcgc ctgaatcacc tttggcgaga acatccgcgt
     2521 caggttggag atgccctcct ccatgatgcg ctcgatttcc gtttttgcaa gcggggcgtt
     2581 tctcgttttc agcgcttcca tgagcttttc atgctgttca atcgagagag aaatgcgctc
     2641 cggcgttgtg aaatagcttg taacatagta caatactacc tgttcgaacg actggaagat
     2701 catggcgtag acgatgtttc cgctggcggc cagcatggcg cggtgaaagc gaaagttcgc
     2761 aacggaaaac tgcgcgggtt cacgggtatt gcgcatttcg tcgagcagga tggagagctt
     2821 ttgaatctca tcgtcgctgg ctttttcggt aaccaagcgc gcgcattcgg tttccagcag
     2881 gcggcgcgtt tccatcatgc tggcaaagag ctccgcgttg gcgtcctccg cgccgtgggt
     2941 catcagcgag agcagcatct gcggcgtgct ctgcgtttta tagtccgcaa caaacgttcc
     3001 ctggcgggga acgatgcgta caaagcccat gctctccaga tcgagaaccg caaggttgac
     3061 gaggctccgg cttacgccca gcagttttgc cagatcccgc tcgggcggca gacgttcacc
     3121 aacggcaagc tcgccggaga agatgcggcg ctgcaattcc cgcgtgacct gctcgcggat
     3181 gctttcggga gtaaatgttc cgattgccgt atgtttcatg gatatcctcc aacagtatct
     3241 tcggtatgct tacaggaaat aatcgtggct tagccactgg ccatatcata gcgcattccc
     3301 ggtcaatccg caaggagtac tttttttagt agtttctgca ggactgctct tttcaacatg
     3361 taaatcgaat cttgaggctt ccgtttccga cagccaaact gtctcttata agcatacagg
     3421 agccatgctg tcttgggtac cgtttcattc gcggcgggcg tataatggaa ccgaccgggc
     3481 agagcgcgta tttaccgaga aaaacacccg gcagcaaggg ggaaacaaac atgacggatc
     3541 gcaaacggtt gattgttaca atactgattc tcgtcgcagc cgcgggcgca tttgtgctcg
     3601 gcctgattat tctgccggat acgctggtga tgcaggtgac ggcaaacggc cagcccggaa
     3661 cgcagatgcc caagctgatc gggcttttga ttccgctggc gctcagcacg gtgtttagca
     3721 ttctttactg caagcgggac ggcgcgaaaa accttattgt tgctctcgtc ggaataggca
     3781 tgtctgcgct gacgttcgcc ttcaacctct aacgcgcgaa aagccgcaca gcaccgcgct
     3841 tttttacgat ttcgccggaa gcgttcaaaa ataatatacg atttttcgca aaaaagtatt
     3901 gacagcgctt tcggacgatg ttagtatacg caccaagaca aacctgcgat tgagaagagt
     3961 agtccaaacg acttggcgtc aagagagccg gcgatggtgg gaatccggcc gcgaaacgat
     4021 ggatgaatgg gctcatgagg gtccgctaac tcccgtttgg ggacgatacg gaacgtaggc
     4081 cgcacgttac agcggagcgc gtatgttggt acgcggcaga gagcgtccga ttcttcgggc
     4141 gaatgcaggt ggcaccgcag gagaaataca tcctgtccta tcgaaaaaac gatagggcag
     4201 gattttttta tgctgattca gacagtttcc ggacgactca aactgccggg caggcgatgg
     4261 cgaagggcat ttcaacccgc cgcgcttacc ccgataacaa acgaaattta aaacaatgtt
     4321 ttacttaaaa aaggagttta gtatgaaaaa cacgctgaag aaaattaccg catccgttct
     4381 ctcaatcctc ctggtactcg ccctgtttgc gggctgcacc gccgctcccg cacccgaagc
     4441 aaccgaagcg cccgccgcga ccgaagccgc agtggaagca atcgcaacgg aagcacccgt
     4501 cgaggaaacc ttcaccgtag gcgtcctgca gttcggcagc catccttcgc tggacaactg
     4561 ttacaacggc attgcggaag ccctctccgc cgacccgcgc attaccatcg acctgcagaa
     4621 cggcaacttc gactccccga cctgcgacac cattgccaaa aacatggctg ccaagcagta
     4681 tgacctgatc atcgcaatcg caactccggc cgccatctcc gcatatgccg cgacggccaa
     4741 tacggatatc ccggtcatct tctgcgccgt gtccgacccc gttgccgcgg gtctggtgga
     4801 cgacatgaac gcgccgggcg gcaactgcac cggaaccagc gacgtgctcg acctctccgc
     4861 gcagctggag ctcatccgcg ccttccagcc ggacgccgtc aaccttggag ttctgtacac
     4921 cacgagcgaa gcaaactccg tttcccagct caaaacgctc aacgaactcg ccccgcagta
     4981 cggctttacg gttgtttccc agggcgtgca gtccgcggcg gatatcccgc aggcggcggc
     5041 aaccctctgt gctcaggtag actgcctgac aaactttacg gataacaacg tggtcaacaa
     5101 cctcgccatc ctgctcgaac aggccaacgc cgcgggcgtg ccggtctacg gttccgagga
     5161 aacgcaggtg accgcgggct gcctggcctc cgtgagcctc gactaccttg cgctcggtcg
     5221 cgtgaccggc cagatggcgc tggatgtact gggcggcgcc gacgcctcca ctatggcggt
     5281 caagaccatt acggacgcgg ttccggttgt caacagtgat gtgctcgcgg ccttcggtta
     5341 caccctgccg gaagcgtacg ctaatgcgac gctcgtaacc acgacagccg aataacaaaa
     5401 gtaaaaaagc aatcgcgcaa attgcggaag gctggtttac cggccttccg cggagcgttt
     5461 tattgcaggg gctagatgaa tcgtttgtcc ggcccggata ccgattcgtt ccggcaggcc
     5521 gtgtgatggc gagcgccgga gaaacggaaa gcaggttaac tggaatggaa attattctca
     5581 acgtattgaa ggtaacgctc gaagaggggc tgatgtacgc gctcctcgcc tttggcg
//
LOCUS       DAOMGY010000010         5596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_14677_length_5596_cov_3.197076, whole genome shotgun sequence.
ACCESSION   DAOMGY010000010 DAOMGY010000000
VERSION     DAOMGY010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14677_length_5596_cov_3.197076"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..1171)
                     /locus_tag="PLR57_00355"
     CDS             complement(263..1171)
                     /locus_tag="PLR57_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HQQ39934.1"
                     /translation="MLKKVPLLILSLVVLLAFTACGGAKIDGSSDGMLAVSVTFDALG
                     EFAKAVGGDKVAVSVIIPTGTEPHDFEPKAQDLVGLSSADVFVYNGLGMESWATDAVS
                     AANNEKLIAVDASTGADVIENTGEGVIAERGQYDPHLWLGIKGAETEVTNIKNAFVQA
                     DPANKAYYEANAKSYIDQLEALYNGYSAKFAAVEKKSFVTGHAAFGYLCRDFGLTQNS
                     VEDVFAEGEPSAQQLAELVDYCRQNGVTTIFAEEMASPEISQTLANEVGASVKTIYTM
                     ESAEDGLSYLERMTDNLSEIYDALSK"
     gene            1325..1993
                     /locus_tag="PLR57_00360"
     CDS             1325..1993
                     /locus_tag="PLR57_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HQQ39935.1"
                     /translation="MIKAEGLFFSYTGSAPYVLNGIDLEIKDGEYISLVGDNGSGKST
                     LVRLMLRFLKPTSGTISCNARRVGYVPQKNDNANSDFPITVFEALSSYRRILRVADRS
                     AVTRCLERVGMIDFSGERVGTLSGGQAQRVMIARALIGQPDLLILDEPSSGVDADSQK
                     EIYGFLKQANQQDGMTIVSVEHNIDAAVQNSTQIYHLSGGRGHLCSPRHYAEEFLHGR
                     RKES"
     gene            1999..2811
                     /locus_tag="PLR57_00365"
     CDS             1999..2811
                     /locus_tag="PLR57_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HQQ39936.1"
                     /translation="MFQYAFMQNAFLVSICISILCPCIGIFLVLRRYSMIGDTLSHAS
                     LAGITIGLLTNQNPILGAFLFTSICGALIEFLRSYFKKYTDLILTIVLALSVGTAITV
                     ISSGKLHANADSFLFGSILTVTEFDIVMVLILSVISVAALILLYHQMLYIAYDEEAAQ
                     VAGVRVRLINYIFAVLVASAISVSIRIVGVLVLSSMIALPVATALQLGKGFKLTLIFS
                     IIFSVIDIMAGLFLSYYLNVAPGGFTALVSVGVLVLVMAGRRIAVGMRRARA"
     gene            2913..3329
                     /locus_tag="PLR57_00370"
     CDS             2913..3329
                     /locus_tag="PLR57_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQQ39937.1"
                     /translation="MKEAPCAWPAGLKKTKQRESVLAVLEQADQPLSAVEICGRMETG
                     VKPAWLSTVYRVLEQFVEKGIVMKTSVLNSEMAVYELNRFRHKHYAVCVKCHKMIAIE
                     DCPMNNAFPKLEAENFEIMGHNLEIFGYCKDCKHEE"
     gene            complement(3478..3873)
                     /locus_tag="PLR57_00375"
     CDS             complement(3478..3873)
                     /locus_tag="PLR57_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39938.1"
                     /translation="MSSLFLDSFQLIVAGYLLYIAIKGSGTMYRFFDLPEQEQPRIRK
                     LLRTVYLVCGLLALLDAGANMLQNSMFTQQVLESGTTVTQNFTLDGFAFVTFDLLSIV
                     STVLTCVIILALALTFIYLRSVAAKTGKK"
     gene            complement(4042..4791)
                     /locus_tag="PLR57_00380"
     CDS             complement(4042..4791)
                     /locus_tag="PLR57_00380"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQQ39939.1"
                     /translation="MLFDIKGKNAIVTGASRGIGYGIAKGFAEAGANLICICRSMPDD
                     VLEEFRSFGVRAEAYSFDLSEPEKIPALVEKILADWGQIDILVNNAGAQRRNNCADFT
                     DEDWDFVMNVNAKSVFVLCREVGRHMVQRGSGKIVNFASLLSFQGGYRVPAYSASKGA
                     VMQFTKTFANEWAKLGVNVNCIAPGYIDTEMNVALVNDPVRNEQITTRIPAGRWGHPE
                     DLVGAAIFLSSSASDYVHGTTLIVDGGWMGR"
     gene            complement(4803..>5596)
                     /locus_tag="PLR57_00385"
     CDS             complement(4803..>5596)
                     /locus_tag="PLR57_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQQ39940.1"
                     /translation="PGIKKGSLVTVNPLLSCGTCEPCKTGNSHVCKTLKLLGIDCDGG
                     MSEYAIAPIDKIVPVPEGVSEKLGAFIEPIAVAVHALRMAQYQAGDSALVFGAGAIGL
                     ATAVTLRRFGASDITICEPNPVRLEFAKSLGFHCILSGPELLETLMQETNGDGYDFVF
                     DCAGHQSVADILPDVVKIRGKIEIIAGYKKPPAMNYQKGMFKEFSIQFTRVYTHKDFQ
                     IAAKLSAMEPLFERLVNYELPPEEAQKGFDLLTSPSDAIKVMFRF"
BASE COUNT         1231 a         1519 c         1593 g         1253 t
ORIGIN      
        1 cactgtgaaa ctattgagtt catcgggaaa tgtttcaaaa atgattcacg tggcttagcc
       61 accaaccaca tcatactaaa tagcagtgag aaacacaagc gcaaaaagcg ctaatttacg
      121 caaaaatttg ccgtttttgc aatatgcagc aaccgaaagc aagccgacgg tactaaactt
      181 ggatttcgga agaaaaggca acaaaaaaga gccttgcggc tctcttgtaa catgcgggat
      241 atgaaagcaa atgacggcag gatcatttgc tcagcgcgtc gtagatttcc gaaagattgt
      301 ccgtcatgcg ctcgagatag ctcaaaccgt cctccgcgct ctccatggtg tagatggttt
      361 ttacgctcgc gccgacttcg tttgccagcg tctgggagat ctccgggctc gccatctcct
      421 cggcaaaaat agtggttacg ccgttctgac ggcagtagtc cacgagctcg gcgagctgct
      481 gcgcgctggg ttcgccttcg gcaaatacgt cttccacgct gttctgcgtt aatccgaagt
      541 cgcggcagag gtaaccgaac gccgcgtgcc ctgttacaaa gctcttcttt tcgacggcgg
      601 cgaacttggc gctgtagccg ttatagaggg cttcaagctg atcaatatag ctcttggcat
      661 tggcttcgta atacgctttg ttggcggggt cggcctgaac gaacgcgttc ttaatattgg
      721 tcacttccgt ttcagcgcct ttgatgccga gccagaggtg cgggtcgtat tgaccacgct
      781 cggcgataac gccttctccg gtgttttcaa tcacgtccgc gcccgtggaa gcgtccacgg
      841 cgatgagctt ttcgttattc gcggcagaaa ccgcgtcagt agcccagctt tccataccga
      901 gtccgttata cacgaatacg tccgcgctgc tgaggcctac aagatcctgc gctttcggct
      961 caaaatcgtg cggctcggtg ccggtcggga tgatgacgga aacagcgacc ttgtcgccgc
     1021 cgaccgcttt ggcgaattcg ccgagcgcgt cgaacgtaac ggaaacggcg agcattccgt
     1081 cgctcgaacc gtcgattttc gcgccgccgc acgcggtaaa cgcaagtaaa acgacaagac
     1141 tcaggatcaa aagagggact tttttcagca cggttatgac ctccggggga aattcttctt
     1201 gcaaatgcga tccattcgca tttatactat tgcatgatac gtgacatagg acgatttgtc
     1261 aagcgcaaat ggtacgcatt cgcaattaaa ccgggcagat gcccgcatgc aagaaggaac
     1321 tggaatgatc aaagcggaag gcttgttttt ttcctatact gggtcggctc cgtatgtact
     1381 gaacggaatc gatctggaaa tcaaggacgg cgagtacatt tccctcgttg gcgacaacgg
     1441 cagcggaaag agcacgctcg tccgcctgat gctgcggttt ttaaaaccga cgagcggaac
     1501 gatttcctgc aacgccagac gcgtaggcta cgtgccgcag aagaacgaca acgccaactc
     1561 cgattttccc attacggtat tcgaagcgct gagttcctac cggcggattc tgcgtgttgc
     1621 cgacaggagt gccgtcacgc gttgtctgga acgcgtcggc atgattgact tttccgggga
     1681 acgcgtgggc acgctttccg gcgggcaggc gcagcgcgtc atgattgcgc gcgcgctgat
     1741 cggccagccc gatctgctga ttctcgacga gccgtcctcc ggcgtggacg cggacagcca
     1801 gaaggagatc tacggctttt tgaagcaggc caaccagcag gacggcatga ccatcgtctc
     1861 cgttgagcac aatatcgacg cggcggtgca gaactctacc cagatctacc atctctccgg
     1921 cgggcgcggg cacctctgct cgccgcggca ttacgccgaa gaatttttgc acggcagaag
     1981 aaaggagagc tgaagaggat gtttcaatac gctttcatgc agaacgcgtt tctcgtttcc
     2041 atctgcattt ccattctctg cccctgcatc ggcatctttc ttgtgctgag aaggtattcc
     2101 atgatcgggg acacgctatc gcatgcgtct ctggcgggca ttacgatcgg gcttttaacc
     2161 aaccagaacc cgatactcgg cgcgtttctg tttacctcca tctgcggcgc gctgatcgag
     2221 ttcttgcgca gctactttaa aaagtacacc gacctgattc tgaccatcgt gcttgcgctc
     2281 agcgtcggca cggcgattac cgtcatcagc tccggcaaac tgcatgccaa cgcggactca
     2341 tttctctttg gcagcatcct caccgtaacg gagttcgata tcgtcatggt gctgattctc
     2401 agcgtcatct cggttgcggc gctaatcctg ctgtatcacc agatgctcta cattgcctac
     2461 gacgaagaag cggcgcaggt ggcgggcgtc agggtgcggc tgatcaacta catctttgcg
     2521 gtactcgtgg catcggctat ctctgtctct atccgcatcg tcggcgtgtt ggtgctcagc
     2581 tcgatgattg cgctgcccgt tgctacggcg ctccagctcg gcaaggggtt taagctgacg
     2641 ctgattttct ccatcatatt cagcgtgatc gacatcatgg cggggctgtt tctttcctat
     2701 tatttgaacg tcgctcccgg cgggtttacg gcgctcgtct cggtgggcgt gctggtgctc
     2761 gttatggcgg gcaggcgaat tgcggtcggg atgcgacggg cgcgcgcttg acgcgtcgtt
     2821 cgggcgcatt ctataatgga tgcgcagttt tgcgagtttg ttaaagatat gcggcagcgt
     2881 ccgcgagaaa acggcggaaa gaggggagta cgatgaagga agcgccatgc gcctggcctg
     2941 cggggttgaa aaaaaccaaa cagcgcgaaa gcgtgctcgc cgtgctggaa caggccgatc
     3001 agccgctcag cgccgttgaa atctgcggcc ggatggaaac gggggtcaag cccgcctggc
     3061 tttccaccgt ttaccgcgtg ctcgagcagt tcgttgaaaa gggcattgtc atgaaaacga
     3121 gcgtgctcaa cagcgagatg gcggtgtacg agctcaaccg tttccggcac aagcactatg
     3181 ccgtctgcgt caagtgtcac aaaatgatcg ccattgagga ctgcccgatg aacaacgcct
     3241 tccccaagct ggaagcggaa aatttcgaga tcatggggca taatctggag atttttgggt
     3301 attgtaaaga ctgcaagcac gaagaatagt gccggtcgaa ctgtattaga ttgataaaaa
     3361 agactgcaag cttgaagagt aactcaattt aggaaaacag cacctactgt gcggaatatt
     3421 tataagcaac aaaaaacgaa cctcgcgcga ggttcgtttt tatttcggag aaatccttta
     3481 ctttttaccc gttttcgccg caacggagcg gaggtaaata aacgttagcg cgagcgccag
     3541 aataattacg caggtgagca ctgtgcttac gatggagagc agatcgaagg tgacgaacgc
     3601 aaacccgtcc agcgtgaagt tctgggtgac ggtcgttccg ctttcgagca cctgctgggt
     3661 gaacatgctg ttctgcagca tgtttgcgcc cgcgtccagc agcgcaagca ggccgcagac
     3721 gagatagacc gttcttaata gctttcgaat gcgcggttgt tcctgctcgg gcaggtcgaa
     3781 aaagcggtac atcgttccgc tgcccttgat cgcaatatac agcagatagc ccgcgacgat
     3841 gagttgaaag gaatcgagaa acaaactgga caagattagc cctccttggg tgaatcgcca
     3901 tatccgcgcg gcggatcacg cttcactcta ttatcatagc gaatgaacgc ccgcgcgtca
     3961 aaagcaatcg gcgcggcgga aagccgggaa agaggagacc ggctctccgc cagaacgccg
     4021 gattgcgcag ggaaaacttg ttcagcggcc catccagccg ccgtccacga tcagggttgt
     4081 gccgtggaca tagtccgaag cggaactcga gaggaaaatc gcagcgccca cgaggtcttc
     4141 cggatgtccc caacgtcccg cagggatacg cgtagtaatc tgttcgttgc gcacggggtc
     4201 gttgacgaga gccacgttca tttccgtatc gatataaccc ggcgcgatgc agttgacatt
     4261 aacgccgagc tttgcccact cgttggcgaa cgtcttggta aactgcatga ccgcgccctt
     4321 gctcgcggaa tacgcgggca cgcggtagcc gccctgaaac gagagcaggg aagcgaagtt
     4381 gacaattttg ccggaacccc tctgcaccat gtgccgcccg acttcgcggc agagcacaaa
     4441 cacgctcttg gcgttgacgt tcatcacaaa atcccagtcc tcgtccgtaa agtcggcgca
     4501 gttgtttctg cgctgcgcgc cggcgttgtt gacgaggatg tcgatttgtc cccagtcggc
     4561 gagaattttc tccaccagcg cgggaatctt ctccggctcg gagaggtcga acgaatacgc
     4621 ttccgcgcgg acgccgaacg aacggaactc ttcgagcacg tcatcgggca tgctgcggca
     4681 gatgcaaatc aggtttgcgc ccgcttcggc aaagcctttg gcgatgccgt agccgatgcc
     4741 gcggctcgcg ccggtaacga ttgcgttttt gcctttgatg tcaaacagca tttttcttct
     4801 ccttaaaacc gaaacatgac tttgatcgcg tcggaaggcg atgtcaacag gtcaaagccc
     4861 ttctgcgctt cttccggcgg cagttcgtag ttgacgaggc gctcaaagag aggttccatg
     4921 gcggagagct ttgccgcaat ctggaagtcc ttatgcgtat atacgcgcgt gaactgaatg
     4981 gaaaactcct tgaacatgcc cttttgatag ttcatggccg ggggcttttt gtaacccgcg
     5041 atgatttcaa tcttgccgcg aatcttgacc acgtccggca ggatatccgc aacggactga
     5101 tgccccgcgc agtcgaacac aaaatcgtag ccgtcgccgt ttgtttcctg catgagcgtt
     5161 tcgagcagtt ccggcccgct caaaatacag tgaaagccga ggcttttggc aaactccaaa
     5221 cgtacggggt tgggttcgca gatggtgatg tcgctcgcgc cgaaacgccg cagggtgacg
     5281 gcggtggcaa ggccgatagc gcccgcgccg aacaccagcg cgctgtcgcc cgcctgatac
     5341 tgcgccatgc gcagcgcgtg gacggccacg gcgatgggct cgatgaacgc gccgagcttt
     5401 tcggaaacgc cttccggtac gggcacgatc ttatcgatgg gggcgatggc gtattcggac
     5461 atgccgccgt cgcagtcgat gccgagtagc ttgagggttt tgcagacgtg cgagttgccc
     5521 gttttgcagg gttcgcaggt tccgcaggag agaagcgggt tgaccgtgac cagcgagcct
     5581 tttttaatgc cgggaa
//
LOCUS       DAOMGY010000011         5331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_15615_length_5331_cov_4.262699, whole genome shotgun sequence.
ACCESSION   DAOMGY010000011 DAOMGY010000000
VERSION     DAOMGY010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15615_length_5331_cov_4.262699"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..617)
                     /locus_tag="PLR57_00390"
     CDS             complement(51..617)
                     /locus_tag="PLR57_00390"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006789955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQQ39941.1"
                     /translation="MIILASSSPRRRELMTMIGLDYRIETSGEEEVQPVGLPPAEYVK
                     TLAVQKALPVAQLHPSDCVIGADTIVLLEGEILGKPHTPDVAVQYLTRMQGRTHIVYT
                     GLAALKNGHVDVRHCETTVTFAHMTQSEIEAYVATSEPLDKAGAYGVQGPGGVFVERI
                     EGNYFNVIGLPLPLLYRMLIDAGEIKQG"
     gene            complement(663..1421)
                     /locus_tag="PLR57_00395"
     CDS             complement(663..1421)
                     /locus_tag="PLR57_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQQ39942.1"
                     /translation="MKLISWNVNGLRACITKGFFDFVKEADPDILALQETKMQPEQFE
                     GETGAYKVYWNSAEKKGYSGTAVFVKEEPLSVRYGIGIPEHDTEGRVITLEYPNFYFI
                     TVYTPNSQDGLRRLEYRMTWEDAFLDYLKALDAVKPIIVCGDLNVAHQEIDLKNPKTN
                     RMNAGFTDEERGKMTRLLGNGFVDTFRHFYPDKTEAYSWWSYIGGARSRNAGWRIDYF
                     IVSDRLKDKIKDSKILSDVLGSDHCPVQLDTEAL"
     gene            1634..2320
                     /locus_tag="PLR57_00400"
     CDS             1634..2320
                     /locus_tag="PLR57_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQQ39943.1"
                     /translation="MSRSLNPAAYARQIADTFLFSGVSESEIAALVSAEGIRVEHFEK
                     DVVIFDRKQNERALGLILYGSCLVTKESETGKMPMSVLRQGDLFGAASLFHREEQYVA
                     RVAAAESAWILLISEEALKGMMRKDFRIAENYLAYLTARIRFLSDRLDGFLPQSVEDR
                     VLQFIRQHAENGLFESEWSVSALAESLRVSRTTLYRAIDKLVLEGKLAKHGRTFRLTA
                     DASNKEKIVH"
     gene            2411..3346
                     /locus_tag="PLR57_00405"
     CDS             2411..3346
                     /locus_tag="PLR57_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ39944.1"
                     /translation="MKKIIAILLTVVLLLAVGCAQKPAEPLKIAALKGPTGMGISYLM
                     EEQSDRYSVELLDAPDTVVGKFVSGEIDVAAVPVNLAATLYNKTEGNVVVICVDTLGV
                     LYVLENGDTVNAFSDLAGKTIQATGQGSTPQFVLDYLLSLNNMTDQVTVEYVGEHTAL
                     AALLASGESTLGMLPEPFVSSVTLKNADTRIAIDLNQEWEAATGTKLVQGVYIANREY
                     YNENSKVIAQFLTDYLDSVNRVNTSADAPELIAKLGIVASADIAKVAIPRSNIVCITG
                     EDMVNSATAVLNILFNANPASVGGVLPGDDLFYAG"
     gene            3348..4091
                     /locus_tag="PLR57_00410"
     CDS             3348..4091
                     /locus_tag="PLR57_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQQ39945.1"
                     /translation="MPKKHVWLKKALIALIYLAVWEAASLLVGRELLLPAPLATVRRL
                     FELLAKGESWAYAGLSLLRIMSGYLLGVLAGVLLAVLTARSRFAESLLSPLRGVVKAT
                     PVTSFILLALLWLNSSLVPLFISFLMVLPMVWADTADAILHTDPRLVEMGRVFGLSRT
                     KIVRKIFVPSVLPQFLAACTTSLGFAWKSGVAAEIIALPLRSIGYQLYEAKLRIETLD
                     LFAWTLLVILMSMGLEWLLVRGMRRIRRD"
     gene            4084..4665
                     /locus_tag="PLR57_00415"
     CDS             4084..4665
                     /locus_tag="PLR57_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ39946.1"
                     /translation="MIKLKNISLAFDGRPVFMDVSLEIPAQGVTVFRGPSGAGKTTLF
                     RLLLGLQKPDCGTIEGFPFKKPAVVFQEDRLLPWASALENAALVSDAETAEQTIGELG
                     LCENLCQLPRELSGGMRRRVALARALSYGGDALFLDEPFTGLDEANKEIAVKAILSSG
                     VPVYVITHDDAEAAFFGDHSTVTIRPQSGEKLA"
     gene            4747..>5331
                     /locus_tag="PLR57_00420"
     CDS             4747..>5331
                     /locus_tag="PLR57_00420"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ39947.1"
                     /translation="MKFTLREKRFPSATGLCDIRYRMWIPEDPHAALQITHGMAEHID
                     RYEEFASFLAENGILVYGNDIVSHGKSIRDGVPKGYFGEKNGWDNVVQDMRTMRELVK
                     ADYPGIPFILLGHSMGSFLARTYAGRDGADFDAFIFSGTAGSNPVLGIGKLIAKSEIK
                     KTGGKQPSITLFNMSFGSYNKAFKPNRTVNDWLSR"
BASE COUNT         1135 a         1454 c         1516 g         1226 t
ORIGIN      
        1 attcattcgt gctcttcata actctttttc aagagtgtaa agtgttcgct tcagccctgc
       61 ttgatttccc ccgcgtcgat gagcatgcgg tacagcagcg gcagcggcag gccgataaca
      121 ttgaagtagt tgccttcaat ccgttccaca aacacgccgc ctggcccctg tacgccgtag
      181 gctccagctt tgtcgagcgg ttcgctggtg gcgacgtaag cttcaatttc gctttgtgtc
      241 atgtgtgcga acgtgacggt cgtctcgcag tggcgaacat ccacatgacc gttcttgagc
      301 gccgcaagcc cggtgtagac gatgtgtgtt ctgccctgca tccgcgtaag gtattgcacc
      361 gcaacgtccg gcgtatgggg tttgccgagg atctcgcctt caagcaacac aatcgtatcc
      421 gcgccgatca cgcagtcgct tggatgcagt tgggcaacgg gaagcgcttt ttgcaccgcg
      481 agggttttta catactccgc gggcggcagg ccgacgggct gaacttcctc ttctcctgac
      541 gtttcgattc tgtaatccag gccgatcatg gtcatcagtt cgcggcggcg cggggaagag
      601 gaggcaagaa tgatcatatt ggtacaagtt ctcctgtgtg aaatctagtt aagttctttg
      661 tgttacaagg cttccgtatc aagttgaacg gggcaatggt cgctgccgag cacatccgac
      721 aggattttgc tgtctttgat cttgtctttc aaacggtctg aaacgatgaa gtagtcgatg
      781 cgccagcctg cgttgcggct gcgagcgccg ccgatatagc tccaccagct gtaggcttcc
      841 gttttgtcgg gatagaaatg gcggaacgta tccacaaagc cgttcccaag caggcgcgtc
      901 atctttccgc gttcttcgtc cgtaaagccc gcgttcatgc ggttcgtttt cggattcttc
      961 aggtcgattt cctggtgggc gacgtttaaa tccccgcaga cgatgatcgg cttgactgca
     1021 tccagcgctt tgaggtaatc gaggaacgcg tcttcccacg tcatgcggta ctccagccgg
     1081 cgcaggccgt cctgcgagtt cggcgtatag acggttataa agtaaaagtt cggatattcg
     1141 agcgtaatga cccggccttc cgtatcgtgt tcgggtattc cgattccgta gcgcacggag
     1201 agcggttctt ccttgacaaa aacggcggta cctgaatagc ctttcttttc cgcgctgttc
     1261 cagtaaacct tgtacgcgcc ggtttcgcct tcaaactgct ccggctgcat tttggtttcc
     1321 tgcagagcga ggatgtcggg gtctgcttcc tttacaaaat caaaaaagcc cttcgtaatg
     1381 caggcgcgaa ggccgttgac gttccaggaa atcagcttca tactcgttgc tcctttaccg
     1441 cggatattcc cattcaatat agcatatgtc gccggcaatg acaaacaaac gtttgaaacg
     1501 atcaccggaa aaaagattta cgcgagctgt aataaaattc aaaataaatc gataaataat
     1561 gatcgaatac cattgacaag tcaacgtgtt gtgataaaat gtaccatatg aaacagaaag
     1621 agaggttatc cgtatgagcc gctcattaaa tcccgccgcg tacgcccgtc agattgcgga
     1681 tacctttctg ttttccggcg tttcggaaag cgaaattgcc gcgcttgtct cagcagaggg
     1741 cattcgggtg gagcatttcg aaaaagacgt cgtcatcttc gaccgcaagc agaacgagcg
     1801 tgcgctgggg ctgattctct acggcagctg cctggtgaca aaagagagcg aaacaggcaa
     1861 gatgccaatg agcgttctgc ggcagggaga cctctttgga gcggcttcgc tgtttcatcg
     1921 ggaagagcag tatgtcgcgc gggtggccgc ggcggaaagc gcctggattc tgctcatctc
     1981 cgaggaagcg ctcaaaggca tgatgcgcaa ggactttcgc attgcggaga actatcttgc
     2041 gtacctgacc gcgcggattc gcttcttaag cgatcggctg gacggatttt tgccgcagag
     2101 cgtggaggac cgtgttctcc agttcatccg tcagcacgcg gaaaacggcc tgttcgagtc
     2161 tgaatggagc gttagtgcgc tcgctgagtc gctccgcgtc agccggacga cgctctaccg
     2221 cgccatcgac aaactcgttc tggaaggcaa gctggcaaag catggcagaa cgtttcgcct
     2281 gacggcggac gcttccaaca aggagaaaat cgttcattag ctgcgtctct gcacaactcg
     2341 tattcaactg aagtttcaca aaatttcaaa tagcgccggc gggatgtccg ccggaaaaag
     2401 gagaagaatc atgaaaaaga tcatcgcaat tctgttgact gttgttctgc tgctcgccgt
     2461 cgggtgcgcg cagaagcccg ccgagccgct gaaaatcgcc gcgctcaaag ggccgaccgg
     2521 tatgggcatt tcgtacctga tggaagaaca gtcggatcgt tactctgttg agctgctcga
     2581 tgcgccggat acggtcgtcg gtaagttcgt cagcggggaa atcgacgtag ccgccgtgcc
     2641 ggtcaatctg gccgcgacac tttacaataa aaccgaaggc aacgtcgtcg tcatctgcgt
     2701 ggatacgctt ggcgttctgt acgtgctcga aaacggggat accgtgaatg cgttctccga
     2761 tctcgcgggc aaaactattc aggcgaccgg tcagggcagc acgccgcagt tcgtgctgga
     2821 ctacctgctt tcgctcaaca acatgaccga tcaggtgacg gtggaatacg ttggcgaaca
     2881 caccgcgctt gcggcactgc tggccagcgg agaatcgacg ctcggcatgc tgccggagcc
     2941 gttcgtttcc tccgtgacgc tcaaaaacgc ggacacccgc atcgcaatcg acctgaatca
     3001 ggaatgggaa gccgcaacgg gcacaaagct cgtgcagggc gtttacattg ccaaccgtga
     3061 atactacaac gagaattcga aagtgattgc gcagttcctt acggattatc tggattccgt
     3121 caaccgcgtg aacacgagcg ccgacgctcc cgagctgatt gccaagctgg gcatcgtcgc
     3181 ttcggcggat atcgccaaag tcgccattcc gcgcagcaac atcgtctgca tcacgggtga
     3241 agacatggtc aactccgcaa ccgccgtgct gaacattctc ttcaacgcca acccggcttc
     3301 cgtcggcggc gtactcccgg gagacgacct cttctacgcg ggctgatatg ccaaaaaagc
     3361 atgtctggct gaaaaaagcg ctgattgcgc tcatctatct ggcggtttgg gaagcggcaa
     3421 gcctgctcgt cgggcgggaa ctgctgctcc ccgcgccgct ggcaacggtc cggcggctct
     3481 ttgagcttct ggcaaaaggc gagagctggg cgtatgccgg gctctcgctt ctgcgcatca
     3541 tgagcgggta tctgctcggc gttctcgcgg gcgtactgct ggcggtgctg accgccagga
     3601 gccgctttgc cgagagcctg ctttctccgc tccgcggggt ggtcaaggcg accccggtaa
     3661 cttcgtttat cctcctggcg ctgctctggc tgaactccag cctagtgccg ctgtttattt
     3721 cgtttcttat ggtgctgcct atggtctggg cggatacggc ggacgcaatt ctgcataccg
     3781 acccgcgcct tgtcgaaatg ggccgcgtgt ttggactttc ccgcacgaag attgtgcgaa
     3841 aaatctttgt gccgtccgtt ctgccgcagt ttctggcggc ttgcaccaca tccctcggtt
     3901 ttgcgtggaa atccggcgtt gcggcggaga ttatcgcgct gccgctgcgc tccatcggct
     3961 atcagcttta cgaagcgaaa ctgcgaatcg aaacgctcga tctgtttgcc tggacgctgc
     4021 tggtcattct gatgtcaatg gggctggaat ggctgctggt gcgcggcatg aggaggatac
     4081 ggcgtgatta agcttaaaaa tatctcactc gcctttgacg gccgtccggt ctttatggat
     4141 gtttcgcttg aaattcctgc gcagggcgta acggtctttc gcgggccgtc cggcgcgggc
     4201 aagacgacgc tgtttcggct gctgcttggc ttacaaaagc cggactgcgg cacaatagaa
     4261 ggcttcccgt tcaaaaagcc tgccgtcgtg tttcaggaag accgcctgct gccttgggca
     4321 agcgcgctgg aaaacgccgc gctcgtttcc gacgcggaaa cggcggagca gacgatcggc
     4381 gaactgggat tatgcgaaaa cctctgccag ctcccgcgcg agctatccgg cggcatgcgg
     4441 cggagggttg ctcttgcgcg cgcgctgagc tacggcgggg acgcgctgtt tctggacgaa
     4501 ccctttaccg gactggacga agcgaacaag gaaatcgccg tcaaagcgat tctcagttcg
     4561 ggcgtgccgg tttatgttat cacgcacgac gacgcggaag cggcgttctt tggcgaccat
     4621 tcaaccgtta cgattcgtcc gcagagcggg gaaaaacttg cataaacaat caaacacgat
     4681 atgaaatatt gagtattccg ccggatggtg gtatactttt ttattattat cggggaggtt
     4741 tggagtatga aatttacgct gcgcgaaaaa aggtttccct ccgcgaccgg tctatgcgac
     4801 atccgttacc gcatgtggat accggaagac ccgcacgccg cactgcagat tacgcacgga
     4861 atggcggaac atatcgatcg ttatgaggaa tttgcatctt ttctggccga aaacggaatc
     4921 ctcgtttacg gcaacgacat cgtcagtcac ggaaagagca ttcgcgacgg cgtaccgaag
     4981 ggctacttcg gcgagaaaaa cggctgggac aacgttgttc aggatatgcg caccatgcgc
     5041 gaactggtca aagcggacta tccgggcatt ccgttcattc tcctcgggca ctctatgggt
     5101 tccttccttg cgcgtacgta cgccgggcgt gacggcgcgg atttcgacgc gttcatcttt
     5161 tccggcacgg cggggtcgaa ccccgttctc ggcatcggca agctgattgc caagagcgaa
     5221 ataaaaaaga ccggcggaaa acaaccgagc attacgctgt tcaacatgag cttcggctcg
     5281 tataataaag ccttcaaacc caaccgcacc gtcaacgatt ggctctcgcg c
//
LOCUS       DAOMGY010000012         5297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_15745_length_5297_cov_3.593285, whole genome shotgun sequence.
ACCESSION   DAOMGY010000012 DAOMGY010000000
VERSION     DAOMGY010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15745_length_5297_cov_3.593285"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PLR57_00425"
     CDS             <1..876
                     /locus_tag="PLR57_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HQQ39948.1"
                     /translation="FIGLGDQYLINPGFWTYATIGICSTAGTAFLMWLGERITEDGVG
                     NGVSMLIFASIVSRVPNTLIGWGKELVVGTMPVWQFAVLIIVVVGLITLVTHVDQAQR
                     RIPVQYAKRVVGRKMYGGQSTYLPIKANANGVLPLIFAMTFIQFPGMIAQFWPNGSFY
                     TFYQTYLGSSTTIYFLIYALLIVGFGFFYSSITFNPIEMSKNLQQNGGFITGIRPGKP
                     TSDYLAKISGRLTMFGSLFLAVVAILPTLALRFLGQTSSFGATSVLIMVSVALETTSQ
                     LESHMLMRHYKGFLN"
     gene            1015..1632
                     /locus_tag="PLR57_00430"
     CDS             1015..1632
                     /locus_tag="PLR57_00430"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQQ39949.1"
                     /translation="MKLIFLGPPGAGKGTQAVKIAARFGIAHISTGDMLRAEMREGTE
                     LGKAAKDLIDRGELVPDDVILGMVKNRIQQDDCKNGFLFDGFPRTIAQAEALEAISAI
                     DHVINIDVPVETLIRRISGRRMCSACGAGYHVSTYSETNCTCGAALYQRDDDKEETVR
                     NRLVVYERSTKPLIDYYQKKGLLHTVNGNADIDTVDAEIRAVISK"
     gene            1629..2399
                     /gene="map"
                     /locus_tag="PLR57_00435"
     CDS             1629..2399
                     /gene="map"
                     /locus_tag="PLR57_00435"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQQ39950.1"
                     /translation="MSGITIKTAEDIQKMEAAGRIVEDTLNLLSKAACEGMRTVELDE
                     MAAEFIKSRGATASFLNYNGYPKSICTSVNEEVVHGIPGKYRLKDGDILSLDVGAYLN
                     GFHGDAARTVLIGNVPEEVQKLVRVTKECFFEGLRFAKAGYRISDIAKAIQAHAESHG
                     YGVVRELVGHGIGRDMHEDPEVPNFWTAGRFGVNPRLTPGMTIAVEPMINLGTPRVFQ
                     LDDGWTVVTADKKPSAHYENTLVITDGEPRLLTLHEEG"
     gene            2404..2688
                     /locus_tag="PLR57_00440"
     CDS             2404..2688
                     /locus_tag="PLR57_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HQQ39951.1"
                     /translation="MNEPAIGAICVSRAGRDKGRAFVIVGISDEQHVLLCDGETRKLS
                     RPKKKKLMHCRVEPRRADEIGRRVTEGKPLLDADVRKALNALGYNVYSNN"
     gene            2699..2920
                     /gene="infA"
                     /locus_tag="PLR57_00445"
     CDS             2699..2920
                     /gene="infA"
                     /locus_tag="PLR57_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQQ39952.1"
                     /translation="MSKQDVIEVEGVVSDSFPNAMFEVKLQNGHKILATISGKLRMNF
                     IRILPGDKVTVELSPYDLSRGRITWRGKN"
     gene            2961..3074
                     /gene="rpmJ"
                     /locus_tag="PLR57_00450"
     CDS             2961..3074
                     /gene="rpmJ"
                     /locus_tag="PLR57_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HQQ39953.1"
                     /translation="MKVRPSVKPICEKCKIIKRKGRVMVICENPKHKQKQG"
     gene            3194..3562
                     /gene="rpsM"
                     /locus_tag="PLR57_00455"
     CDS             3194..3562
                     /gene="rpsM"
                     /locus_tag="PLR57_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015847199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQQ39954.1"
                     /translation="MARISGVDLPRDKRIEIGLTYIFGIGRKTATEILAATGVDPDVR
                     VRDLSESDVSKLRDYIDHNVKVEGDLRRETSMNIKRLIEIGCYRGVRHRKGLPVRGQS
                     TKQNARTRKGPKRTQGGKRK"
     gene            3578..3985
                     /gene="rpsK"
                     /locus_tag="PLR57_00460"
     CDS             3578..3985
                     /gene="rpsK"
                     /locus_tag="PLR57_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQQ39955.1"
                     /translation="MAAAKQVKKTASTRKRRERKNIERGAAHIRSSFNNTLVTITDLQ
                     GNAISWSSAGSLGFRGSKKSTPFAAQMAAETAARGAMEHGLKTVEVYVKGPGSGRESA
                     IRALQTAGLDVNLIKDVTPIPHNGCRPPKRRRV"
     gene            4000..4626
                     /gene="rpsD"
                     /locus_tag="PLR57_00465"
     CDS             4000..4626
                     /gene="rpsD"
                     /locus_tag="PLR57_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQQ39956.1"
                     /translation="MARYTGSVCRQCRREGTKLFLKGDRCYSEKCAINKRHTPPGMHG
                     QGRKKQSEYGIQLREKQKVRRAYGVLESQFRKYYDMASNMRGVTGENMLSLLERRLDN
                     VCYRLGFGDSRPMARQMVNHGHILVNGKKVDIASYLIKDGDVVSIREKSADSEFFKTL
                     KEQGAARSIPKWLELNAEKLTGKVVALPKRDDVDLTIEEHLIVEYYSR"
     gene            4677..>5297
                     /locus_tag="PLR57_00470"
     CDS             4677..>5297
                     /locus_tag="PLR57_00470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQQ39957.1"
                     /translation="MLEIEKPKLECVELTEDYGKFIVEPLERGFGTTLGNSMRRVLLS
                     SLPGVAVTSVRIDGVLHEFSIIEGVKEDVTEIILNLKELCCKMYCDGPKKLIIDAAGE
                     GEVTAKDFLPDSDVVIINPELHIATLDKGAKLHLEVMLEHGRGYVPVERNKRHDMPIG
                     EIAIDSIYTPISKVNFTVENTRVGQITDFDKLTLEIWTNGSIKPDEA"
BASE COUNT         1276 a         1456 c         1569 g          996 t
ORIGIN      
        1 ttcatcggtc tcggtgacca atacctgatc aatccgggct tctggactta cgccacgatc
       61 ggcatctgct ccaccgccgg caccgcgttt ttgatgtggc ttggcgagcg gattaccgaa
      121 gacggcgtcg gcaacggtgt ttcgatgctg atctttgctt ccattgtatc gcgcgtaccc
      181 aatacgctga ttggctgggg caaggagctc gtagtcggaa ccatgcccgt gtggcagttt
      241 gcggttctta tcatcgtggt tgtcggactc atcacgctgg tgacgcatgt ggatcaggcg
      301 cagcgccgca tccccgtgca gtacgcaaag cgcgttgtcg gacgcaagat gtatggcggg
      361 cagagcacgt atctgccgat caaggcaaat gccaacggcg ttctgccgct gatctttgcc
      421 atgacgttca tccagttccc gggcatgatc gcccagttct ggccgaacgg ttcgttctat
      481 acgttctatc agacgtatct cgggtcttcg accacgattt actttctgat ttatgcgctg
      541 ctgatcgtcg ggttcgggtt cttctattcg agcattacgt tcaacccgat cgagatgtcc
      601 aagaacctgc agcagaacgg cggcttcatt actggcattc gtccgggcaa gccgacgagc
      661 gactatctgg ccaagatctc cggaaggctc acgatgttcg gttcgctctt cctcgcggtt
      721 gttgcgattt tgccgacgct tgcgctcagg ttccttggcc aaacgagttc gttcggcgca
      781 accagcgtgc tgattatggt cagcgttgcg ctggaaacca cgagccagct cgagagccac
      841 atgctcatgc ggcactacaa gggcttcctc aactaaggaa agcggaatcg tccttcggga
      901 tgtttgcaaa aggattggcg gacggcgccg cctgaaacat ggcgcgcgcc ggctccggca
      961 tccggatggg acgggcacgg cggacgaacg ccgcgcaaac cgggaggaaa tgttatgaag
     1021 ctgatctttt taggcccgcc aggcgcgggc aaggggacgc aggcggtcaa gatcgccgcc
     1081 aggttcggca ttgcccatat ttccacggga gatatgctcc gcgcggaaat gcgggaaggc
     1141 accgagctcg gcaaggctgc aaaagacctg atcgaccgcg gcgaactcgt gccggacgac
     1201 gtgatcctcg gcatggtgaa gaaccgcatc cagcaggacg actgcaaaaa cggtttcctg
     1261 ttcgacggtt ttccgcgtac gattgcgcag gcggaagcgc tggaagcaat cagcgccatc
     1321 gaccatgtga tcaacatcga cgttccggtg gaaacgctca ttcggcggat cagcgggcga
     1381 aggatgtgct ccgcttgcgg agccgggtat catgtttcca cgtattcgga aacgaactgc
     1441 acctgcggag ccgcgctgta tcagcgcgac gacgacaagg aagaaacggt tcgcaaccgt
     1501 cttgtggtct atgagcgctc gaccaagccg ctgattgact actaccaaaa gaagggtctg
     1561 ctccacacgg tgaacggcaa tgcggatatc gacaccgtag acgccgagat ccgggcggtg
     1621 atttcgaaat gagcggcatc acgattaaaa ccgcggaaga catccaaaag atggaagccg
     1681 ccgggcgcat cgtggaagat acgctcaatc tcctctcgaa ggctgcgtgc gaaggcatgc
     1741 gaaccgtaga gctggacgaa atggccgcag agttcatcaa gagccggggg gcaaccgctt
     1801 cgtttctcaa ctacaacggg tatcctaaga gcatctgcac ttcggtcaac gaagaggttg
     1861 tgcacgggat cccggggaag taccgtctca aggacgggga catcctctcg ctggacgtcg
     1921 gcgcttacct caacgggttt cacggcgacg cggcacggac ggtcctgatc gggaacgttc
     1981 cggaggaagt tcaaaagctc gtccgcgtga caaaggaatg cttctttgaa ggcctgcggt
     2041 ttgcaaaagc cgggtatcgg atatccgaca ttgcaaaagc gattcaggcg cacgcggaaa
     2101 gccacggcta cggcgttgtg cgcgaactgg tcggccatgg aatcggcagg gatatgcacg
     2161 aggatccgga agtgccaaat ttctggaccg cagggcgttt cggcgtcaat ccgaggctta
     2221 cccccggcat gacgattgcc gtcgaaccca tgatcaacct cggcacgccg cgcgtctttc
     2281 agctggatga cggctggacg gtggtcacgg cggataagaa gccttccgcg cactacgaaa
     2341 acaccctcgt catcacggat ggcgaaccca ggctgctgac gctgcacgag gagggctgaa
     2401 cccatgaacg aacccgcaat cggcgcgatc tgcgtttcca gagcgggccg ggacaaaggg
     2461 agagcgtttg taatcgtcgg aatcagcgat gagcagcatg tgcttctttg cgacggcgaa
     2521 acgcgaaagc tgtcccgccc aaagaaaaag aaactgatgc actgcagggt cgaaccgcgt
     2581 cgcgcggatg agatcggcag gcgtgtgacc gaaggaaaac cgcttttgga cgcggacgtt
     2641 agaaaagcgc tgaacgcgct cggttacaac gtgtattcga ataattagga gggatagctt
     2701 gtctaaacag gacgtcattg aagtggaagg tgtcgtttcg gattcattcc cgaacgcaat
     2761 gttcgaggtg aagctccaaa atggacacaa gatactcgca acgatatcgg ggaaactgcg
     2821 gatgaacttc atccggatcc tgccgggtga taaggtcacc gtcgagctgt ccccgtacga
     2881 cttgtcccgc gggcgcatta cctggcgcgg caagaactga ttttcgaaag attttcgata
     2941 agagcgaaag gagaaccacc atgaaagtaa gaccgagtgt gaagcccatt tgcgagaagt
     3001 gcaaaattat caagcgcaaa ggccgcgtca tggttatctg cgagaacccc aagcataagc
     3061 agaagcaggg ctgacgatcg gacaataccg ctggagcggc tgcgcagcgc ggcatcaccc
     3121 gcgtgcgttt ttccggcgga tggaagatag attcaaaacc atttacgact atttacggag
     3181 gtaaaggaac gatatggcac gtatttctgg cgtagacctt cccagggaca aacggattga
     3241 aatcggtttg acctacatct ttgggatcgg ccgcaaaacc gctacggaaa tattggccgc
     3301 aaccggcgtc gaccccgacg tccgcgtgcg cgacctgtcc gaaagcgacg tttccaagct
     3361 gagagattac attgaccaca acgtcaaagt ggaaggcgac cttcgccgtg aaacttcgat
     3421 gaacatcaag cggctgatcg agatcggctg ttaccgcggc gtgcgtcacc gcaaaggcct
     3481 gcccgttcgc ggacagagca ccaagcagaa cgcgagaact cgcaaaggcc cgaagcgcac
     3541 gcagggcggc aagagaaagt aaggaggata agcgaaaatg gcagcagcga aacaagtaaa
     3601 gaagaccgct tccacgcgga aacgccgcga gcgcaaaaat attgagcgcg gcgcagcgca
     3661 catccgttcc tccttcaaca acacccttgt gaccatcacc gacctgcagg gcaacgccat
     3721 cagctggtcg agcgcaggtt cgctcgggtt ccgcggatcc aagaagtcca ctccgttcgc
     3781 agcccagatg gcagcggaaa ccgccgcccg cggcgcgatg gagcatggcc tcaagaccgt
     3841 tgaagtgtac gtaaaaggcc ccggttccgg ccgtgaatcg gcaatccgtg ccctgcagac
     3901 cgcagggttg gatgtgaacc tcatcaagga cgtgacgccc atcccgcaca acggatgccg
     3961 cccgcccaag cgcagaagag tgtaaggagg gaatgctaaa tggctagata tacaggttcc
     4021 gtctgcagac agtgccgcag agaaggcacc aaactcttcc tcaagggcga ccggtgctac
     4081 agtgaaaagt gcgcgatcaa caagcgccac acacctccgg gaatgcatgg tcagggcaga
     4141 aagaagcaga gcgagtacgg catccagctt cgtgagaagc agaaggttcg cagagcctac
     4201 ggcgtgctgg aatctcagtt ccgcaagtac tacgatatgg cctccaacat gcgcggcgtc
     4261 accggcgaaa acatgctttc gctgctcgaa cgccggttgg ataacgtttg ctatcgcctc
     4321 ggctttggcg actcccgccc gatggcgcgc cagatggtga accatggaca catcctcgtc
     4381 aacggcaaga aggttgacat cgcttcctac ctcattaagg atggcgacgt ggtcagcatt
     4441 cgcgagaaga gcgccgacag cgagttcttt aaaacgctca aagagcaggg cgcagccagg
     4501 tcgatcccca agtggctcga actgaacgcc gaaaagctca ccggtaaggt cgttgcactt
     4561 ccgaagcgcg acgatgtcga cttgacgatt gaagagcatc tcatcgttga gtactactcc
     4621 aggtaagcca agctaaattg gcggaccgca acaaacaaac cgaaggaggg taagcaatgc
     4681 tggagattga aaagcccaaa ctcgaatgtg ttgaactgac ggaagattac ggtaaattca
     4741 tcgtagagcc gttggagcgc ggattcggca ccacgcttgg caattccatg cgccgcgtgc
     4801 tgctctcgag cctgcccggt gtggcggtta cgagcgtacg catcgacgga gtgctgcatg
     4861 agttctcgat tatcgagggc gtaaaagaag acgttaccga aatcattctc aacctcaagg
     4921 agctctgctg caagatgtat tgcgacgggc ccaagaagct gattattgac gccgcaggcg
     4981 agggcgaggt cacggccaag gatttccttc cggattccga cgtggtgatc atcaaccccg
     5041 aactgcatat cgcaacgctg gataagggcg cgaaactcca cttggaggtc atgcttgagc
     5101 atggccgggg atacgtcccc gtagagcgca acaagcgcca cgatatgccc atcggcgaga
     5161 tcgcgatcga ctcgatttac accccgattt ccaaggtgaa cttcaccgtc gagaacacgc
     5221 gcgtcggcca gatcaccgac tttgacaagc tcacgctcga gatttggacc aacggcagca
     5281 tcaagccgga cgaggct
//
LOCUS       DAOMGY010000013         5291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_15780_length_5291_cov_3.636555, whole genome shotgun sequence.
ACCESSION   DAOMGY010000013 DAOMGY010000000
VERSION     DAOMGY010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15780_length_5291_cov_3.636555"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLR57_00475"
     CDS             complement(<1..640)
                     /locus_tag="PLR57_00475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HQQ39958.1"
                     /translation="MEPLVTVILPTHNHAPFIAHAIESVLMQQTSYPFDILLHDDAST
                     DGTADICRQYAAKYPEKITLIAQTVNQYTIDRRIQSHILIPRVKAKYTAILDGDDFWV
                     DPLKLQRQLDYLESHPDCTLCIGAANLVDVKDRVIGRVAPYTESRIVDPADMIRGGGG
                     FTATNTIVMPTQLLKDLPKFADYIEAEDIPFQLLGALKGYAWYDAETLIAYRL"
     gene            complement(643..1590)
                     /locus_tag="PLR57_00480"
     CDS             complement(643..1590)
                     /locus_tag="PLR57_00480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ39959.1"
                     /translation="MEHNAPLVSIVCDTYNHAPYVRAALDSFLMQKTEFPFEIIVHDD
                     ASTDGTADIIREYEAAHPDLFRCIYRTENIYRKDPKILEHYVFPLARGKYVAICEGDD
                     YWTTPDKLQKQIAYMEANPDCTLCVCGADTVDPEGNKTGTLSPYNTDTDAPMDDIIRG
                     SGGFVATAGIVAPTALAKNRAPFCDLSDVDDAVLQIWFGMNGRTHYFAEPMCVYRKAV
                     PGSWTETFFSSKHAERVLHHEAMIRALEAFDAESGFRWHESVEFCTIHQQKYEILRLQ
                     NDCAALRKQPYREQYLALPLRRRVRMQLTRWGILKERKK"
     gene            complement(1638..2741)
                     /locus_tag="PLR57_00485"
     CDS             complement(1638..2741)
                     /locus_tag="PLR57_00485"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HQQ39960.1"
                     /translation="MGLQILVGADAAPAAAAAEATQGKAPAMGESLQTVWESAGARLF
                     SLDCALGACGNEQALAGETAPLCAQGVLRLKPAAVSLFCRNAQNTDGERLGGTLALLK
                     TAGIPYFGAGADLDEAEKPYYFAKNNLRIGVYALSEHSGCAATERSAGTNPLDLVELG
                     DRIRELRGSCDRLIVFYNGGNEAYPYPSPDAQKVCRKAAECGASLVLSRQGDLPGCYE
                     KWDNATIVYGLGAFLGAENAEHEGVLVQYEIGDYGTEHVSFLPVESGESGTEFATGER
                     AEQILEAFETRSRRIRVQGFVEARFRAYAAANRERILHVLSGNGSGRRAVDALTGKSD
                     QTYSREDIQALLGALESESARELLAEGLRHDAV"
     gene            complement(2826..3866)
                     /locus_tag="PLR57_00490"
     CDS             complement(2826..3866)
                     /locus_tag="PLR57_00490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQQ39961.1"
                     /translation="MYFSILVPVYNAEAYLEECVDSALRQTERDFELILVDDGSSDAS
                     GAICDRYAAEFPGRVKVIHQTNRGLILARRAGVAAASGEYCLFLDADDTIAEDTLETV
                     KKTIEREHADVVIYNFYNRYLPDETLDMATPVFEDQTVFRGEGKKKLYDKIISSWRLN
                     NIWSKAIRMPLVKGDDTPFEEYAYNPHMEDLLQSLYPLTHADCVVYLAKPLYYYRRNF
                     LGISGNVVAGQIERQFSEPVMKQLARYMTIWGMDSQQDREKLNVRKVKKMLTVFYQHY
                     RAAETKVQKREVIAIPWGEHIDWENGRIVGRNHLSLSQQVQLFAVLQKRGLLIGLLAR
                     FGQIKLKRLHGD"
     gene            complement(3923..5002)
                     /locus_tag="PLR57_00495"
     CDS             complement(3923..5002)
                     /locus_tag="PLR57_00495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ39962.1"
                     /translation="MKKLMIVTHKMSGGGCERVIAQLLTCFARDGIECKLVTECGVPS
                     FYDLPESVRHIYLSFDKSLRARDIPKAYKKLRKLVLQEKPDVVLAMPEKVNVWTVLFL
                     LGTGVPVVVSERNDPHRHPENKIKRMLRCLVYPFAKGFIFQTEDAAAYFSKKIRSRGI
                     VLDNPLDVSRIPARHDGARRSVVAAAGRLHPQKNFAMLIRAFARFYKTHHSYSLVIYG
                     EGGEKQALMRLASSLGVAGAVEFAGQSKTLLGDINDCAMFALSSDYEGMPNVLIEAMA
                     CGLCCVATDCPIGGVRSLITDGENGLLIGVGDEDAMSRALCAITDDPALAERLGSNAA
                     QIQSRLDETLVAEQWMRYLDRIAEQ"
     gene            complement(5083..>5291)
                     /locus_tag="PLR57_00500"
     CDS             complement(5083..>5291)
                     /locus_tag="PLR57_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39963.1"
                     /translation="LTELVAFTSIERAEEFALAMEQSMARKADRAIMSAVAILHAEEK
                     GYDVSQNAQMLMQIYQSAAAKHRY"
BASE COUNT         1040 a         1596 c         1470 g         1185 t
ORIGIN      
        1 tcaaccggta ggcgatcagc gtttccgcgt cgtaccaggc atagcctttc agcgcgccga
       61 gcagctggaa cgggatatcc tcggcctcga tatagtccgc aaacttcggt aaatccttga
      121 gcagctgggt cggcatgacg atcgtattgg tcgccgtaaa cccgcccccg ccgcgaatca
      181 tgtccgccgg atcgacaata cggctctcgg tatacggcgc gacgcggccg atcacgcggt
      241 cttttacgtc cacgagattc gccgctccaa tgcaaagcgt gcagtccggg tgcgattcca
      301 gatagtcgag ctggcgctga agtttcagcg gatcaaccca gaagtcgtcc ccgtcgagaa
      361 tggcggtata tttggctttg acgcgcggaa tgaggatatg ggactggatg cgcctgtcga
      421 tcgtatactg gttgaccgtc tgcgcaatca gcgtaatctt ctccgggtat tttgcggcat
      481 actggcggca gatatcggcg gtgccgtccg tagaggcgtc gtcatgcagc aggatatcga
      541 aaggatagct cgtttgctgc atcagcacgc tttcaatcgc gtgcgcgata aacggcgcgt
      601 ggttatgcgt tggcaggatg acggtaacaa gcggttccat actcatttct tcctttcctt
      661 caaaatcccc cagcgcgtga gctgcatgcg cacacggcgg cgcagcggca gcgcaaggta
      721 ctgctcgcgg taaggctgtt tacgtaaggc ggcgcagtcg ttttgcagcc gcagaatctc
      781 gtatttttgt tgatgaatag tgcagaattc caccgattcg tgccagcgga atccgctttc
      841 ggcgtcaaat gcttccagcg cgcgaatcat cgcttcatgg tgcaaaacgc gttcggcgtg
      901 cttggaggaa aagaacgttt ccgtccacga gccgggcacg gctttgcggt acacgcacat
      961 gggttctgca aagtaatgcg ttcttccgtt catgccaaac cagatctgca gcacagcgtc
     1021 gtccacgtcc gaaaggtcgc aaaacggcgc gcggttcttt gcaagcgcgg tgggagccac
     1081 gatgccggca gtcgcaacaa acccgccgct gccgcggatg atatcgtcca tcggcgcgtc
     1141 ggtgtccgta ttatacggcg aaagcgtgcc ggttttgttc ccttccgggt caacggtgtc
     1201 cgccccgcag acgcagagcg tgcagtcggg atttgcttcc atataggcaa tctgcttttg
     1261 gagtttatcc ggcgtcgtcc agtaatcgtc cccttcgcag atagcgacgt acttgccgcg
     1321 cgcgagcggg aacacatagt gctccagaat tttcgggtcc ttgcggtaga tgttttccgt
     1381 gcggtagata cagcggaaca agtcgggatg cgccgcttcg tattcacgga tgatgtccgc
     1441 cgtgccgtcc gtagaagcgt cgtcatgcac gattatctca aacggaaact ccgttttctg
     1501 catcaaaaag ctgtccagcg cggcgcgtac gtacggcgcg tggttatagg tgtcgcaaac
     1561 gatggaaacc agcggcgcgt tatgctccat gtgtctcctc cgatccggct ttcggcttgt
     1621 tttccggcat tcttgcgtta taccgcatcg tgccgcaggc cttccgccag cagttcgcgc
     1681 gcggactcgc tttccagcgc gcccagcagg gcttgaatgt cctcgcggct gtacgtttga
     1741 tcgctcttgc ccgtcagcgc gtccacggcg cgtctgcccg atccgttgcc ggaaagcaca
     1801 tgcagaatcc gttcgcggtt tgccgcggca taagcgcgga atcgggcttc cacaaatccc
     1861 tgcacgcgga tgcggcgcga acgcgtttca aacgcttcca atatctgctc tgcgcgttca
     1921 ccagttgcga actcggttcc gctttcgcca gattcgacgg gtaaaaacga aacgtgctcc
     1981 gtgccgtaat cgccaatttc gtattgaacc agaacgcctt cgtgctctgc gttctccgcg
     2041 ccgaggaatg cgccaaggcc gtagacgatg gttgcgttgt cccacttctc gtagcagccc
     2101 ggcaggtctc cctgacggct gagcacgagg gaagcgccgc actcggcggc cttgcggcag
     2161 accttctgcg cgtccggcga gggataagga tacgcttcgt ttccgccgtt gtaaaagacg
     2221 atcaaacggt cgcagctgcc gcgcagttcg cgaatgcggt cgcccagttc cacgagatcc
     2281 agcgggttcg tgcccgccga gcgttcggtt gcggcgcagc cgctgtgctc gctcagcgcg
     2341 tatacgccga tgcggagatt gttcttggca aaatagtacg gtttttccgc ttcgtccaaa
     2401 tccgcccccg cgccaaagta cggaatgcct gcggttttca gcagcgcaag cgtgccgcca
     2461 agacgctcgc cgtccgtgtt ttgcgcattt ctgcaaaaca gcgatacggc ggcgggtttc
     2521 aggcgaagta cgccttgcgc gcaaagcgga gcagtttctc ccgccagcgc ctgttcgttg
     2581 ccacaagcgc cgagcgcgca gtcaaggctg aaaaggcgcg cgcccgcgct ctcccaaacc
     2641 gtctggaggc tctcgcccat cgcaggcgct ttgccctgcg tcgcctccgc cgctgccgca
     2701 gccggggcgg cgtccgctcc gacaagaatt tgtaaaccca tgtttgttcc tccaataaaa
     2761 accccgggga tgttccccaa agatcaattc ctaactaaac agaaattact ctgctaaaga
     2821 ccgcatcagt cgccgtgcag gcgtttgagc ttgatctgcc cgaaccgcgc gagaaggcca
     2881 atcagcaggc cgcgcttttg cagtacggcg aaaagctgca cttgctggga aaggctcaaa
     2941 tgattcctgc cgacgatgcg cccgttttcc cagtcgatat gttcgcccca gggaatggcg
     3001 atgacctcgc gcttttgtac tttcgtctcg gccgcgcggt aatgctgata aaacaccgtg
     3061 agcatctttt ttactttgcg cacgttcagc ttttcacggt cttgctgcga gtccataccc
     3121 caaatagtca tatagcgcgc aagctgcttc atcaccggct cgctgaactg ccgttcgatc
     3181 tgccccgcga caacattgcc ggaaattccc agaaagttcc gacggtagta gtacagcggc
     3241 tttgccagat agacaacgca gtccgcgtgc gtcagcggat aaagcgactg cagcagatcc
     3301 tccatatgcg ggttgtatgc atactcttca aacggcgtgt cgtcgccctt caccagcggc
     3361 atgcggatgg ctttgctcca aatattgttg agccgccagg aggagataat cttatcgtag
     3421 agcttcttct ttccttcgcc gcggaacacc gtttgatctt caaacacggg cgtcgccata
     3481 tcgagcgttt catccggcag atagcggttg tagaagttat agatgaccac gtccgcatgt
     3541 tcccgttcga tagtcttttt gaccgtctcc agcgtatcct cggcaatcgt atcgtccgcg
     3601 tccagaaaca ggcagtattc tccgcttgcg gcggcgacgc ccgcgcggcg cgcgagaatc
     3661 agcccgcggt ttgtctgatg gattaccttg actcgccccg gaaattccgc cgcgtagcgg
     3721 tcgcagattg ctccgctggc gtcgcttgag ccgtcgtcaa cgaggatgag ctcaaagtcc
     3781 cgctccgttt gccgcagagc ggaatctacg cactcctcca aataggcttc tgcgttatag
     3841 acgggaacga ggatactgaa atacatcgtt cggctttcct ccggccggcg ccgcatgaaa
     3901 aactgcggct ttctggtcct gtttactgtt ctgctatccg gtctagatag cgcatccact
     3961 gctcggctac gagcgtttcg tccagcctag actgaatctg cgcggcgttg ctgcccagcc
     4021 gttcggcaag cgccggatcg tccgtaatgg cacaaagcgc gcggctcatc gcgtcctcgt
     4081 cgccaacgcc gattaaaagg ccgttctcgc cgtccgtgat cagcgaacgc acaccgccga
     4141 tggggcagtc cgttgccacg cagcagagcc cgcaggccat ggcctcgatc agcacgttcg
     4201 gcatgccttc gtagtcggaa ctcagcgcga acatcgcgca gtcgttgata tcgccgagca
     4261 gcgtcttgga ctgtccggca aactccaccg cccccgcaac gccgaggctg ctggcaaggc
     4321 gcatgagcgc ctgcttttcg ccgccctcgc cgtagatgac cagcgaatag ctgtggtgcg
     4381 ttttataaaa acgcgcaaac gcgcgaatga gcattgcaaa gttcttttgc ggatgcagcc
     4441 gccccgccgc cgcgacgacc gatcttcttg ccccatcatg tcgcgcggga atgcgcgaaa
     4501 catccagcgg attatccagc acgatgccgc gcgaacggat tttcttcgaa aagtacgccg
     4561 ccgcgtcttc ggtttggaag ataaagcctt tcgcaaacgg gtacacgagg cacctgagca
     4621 tgcgtttgat cttgttttcc gggtgccggt gcgggtcgtt gcgctcgctg accacgacgg
     4681 gaacgcccgt gccaagcaaa aacagcaccg tccagacgtt caccttttcc ggcatggcga
     4741 gcacgacgtc cggcttttcc tgcaaaacca gttttctcag cttcttgtat gctttcggaa
     4801 tatctcgcgc gcgaagcgac ttatcgaacg aaagatagat atgccttacg ctctccggca
     4861 aatcgtaaaa cgaaggcacg ccgcactcgg tgacgagttt gcactcaatg ccgtcccgcg
     4921 caaagcaggt caatagctgc gcaatcacgc gctcgcagcc accaccgctc atcttgtgcg
     4981 ttacgatcat gagttttttc atcgtgcatg tccctttcta ttcagaatca tggaattctg
     5041 ataacgtgca acctacaccc aagtgggaaa tcaggttatg aatcagtatc tgtgcttcgc
     5101 tgccgcggat tggtaaatct gcatcagcat ctgcgcattt tgcgaaacat cgtatccttt
     5161 ttcctctgcg tggagtatcg caacggcgga catgatcgcg cgatccgctt tgcgcgccat
     5221 gctctgttcc atcgcgaggg cgaattcttc cgcccgctcg atggaagtaa aagcgaccag
     5281 ctccgtcaac g
//
LOCUS       DAOMGY010000014         5246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_15977_length_5246_cov_3.000385, whole genome shotgun sequence.
ACCESSION   DAOMGY010000014 DAOMGY010000000
VERSION     DAOMGY010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15977_length_5246_cov_3.000385"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..452)
                     /locus_tag="PLR57_00505"
     CDS             complement(81..452)
                     /locus_tag="PLR57_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HQQ39964.1"
                     /translation="MHELGIVFEIQKRVSAVALENGLELRDIASVVVEVGEASTIVPR
                     YLKECWPAATDGTEMESATLEVEVIVAEVECKKCGNVYEYLKNNRVCPKCGAWECVMV
                     TGQEFNIKEIVVYEEDEPEEP"
     gene            complement(463..3132)
                     /locus_tag="PLR57_00510"
     CDS             complement(463..3132)
                     /locus_tag="PLR57_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ39965.1"
                     /translation="MIGRKKPGVKPGEKGSLKWDDPEYVILEAGVDDDMCLVGLALGS
                     YEKKSAAEVAAIVGKPEAFCHEQLMKLAVYGACFVNCIDGVDMFWTETWIPGTMEMIV
                     NNLDNIKKYPVVGYAMEAYGRVRGPMSSGSFPVGVGLMRVIPIESAIDGESRKASYEE
                     ISTYLEENTIFSVSPCSCRTDREVMGEGCGHLKEDMCIQMGHAAEYYIRTGRGRQITR
                     EEAYEILRRAEENGLMHEIPNTDGPGKTHAICNCCGCGCLSLRTAAMFKNVDMVRSNY
                     VSEVDASKCVACGQCVENCPVNALKLGQKLCSKKPVVSDITTVITPRDEDWTPDKWNP
                     GYRENREDVVDSGTSPCKTNCPAHIGIQGYIKLASQGKYLEALELIKRENPFPAVCGR
                     VCNRRCEQACTRGELDDPIAIDEIKKFIAAQELTSAGRYVPSKRHDYSDYKIAVVGSG
                     PAGLSCAYYLALDGYTVTVFEKEQKLGGMLTLGIPSFRLEKGVLEAEIDILRQMGVQF
                     QTGVEVGKDITLEALRKQGYRAFYVAIGAQGGRKVGVEGEDAKGVLSGIEFLRKINAG
                     ESLKLSGKTIVIGGGNVAVDVARTAVRSGAETVAMYCLESRDIMPASAEEIEEAEGEG
                     ISIHTCFGPKRILTENGKVTGVEFKKCLSVFDADGRFSPKYDEADTVIVEADSVLVTI
                     GQSILWGDLLEGTNAQFNRNGTLQADGFTYQSGQADVFTGGDCCTGPKFAIDAIAAGK
                     QAAISIHRYVHPGQSLTIGRDRREYVELDKKSAVIDDYDHTPRQKPETLDSKEPATRT
                     FRDLRGTFTPEQVQKETARCLGCGVTLVDSYMCVGCGQCTTKCKFDAIHLVRRYDGAG
                     VEFTEMKPVVIKQILKRKGKIAIKKVKRALGAK"
     gene            complement(3293..3958)
                     /gene="hypB"
                     /locus_tag="PLR57_00515"
     CDS             complement(3293..3958)
                     /gene="hypB"
                     /locus_tag="PLR57_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HQQ39966.1"
                     /translation="MFIVYEIKQRVFESNEREADKLRSELKQRKTFLLNLMSSPGAGK
                     TTTLKRTIEALKDELAIGVMEADIDSDVDARTIAETGVRAIQLHTGGMCHLDADMTRQ
                     GLDEFGVDGLDLVFLENVGNLVCPAEFDTGSVKNAMILSIPEGDDKPLKYPLMFSICH
                     VLLVNKIDVLPYFDFDMAALTERAKKLNPDIEIIPICARTGEGIDVWANWLRRQVADW
                     KRD"
     gene            4259..4957
                     /locus_tag="PLR57_00520"
     CDS             4259..4957
                     /locus_tag="PLR57_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQQ39967.1"
                     /translation="MFKKLTAPTLKQLFVQELESMILSGELAIGQKLPPERELAERMQ
                     ISRSVVNDGIAEMARKGFLEISPRQGTVVADFHRNGTLEILVSIMSYNGGQLPRDEIR
                     SILELRGVLMAFALESAIPLITPEQLARLNEQSEAFVNAPSPEEAATRIYTFDHMLSG
                     FSGNTLLPLIFCSFRAPIKELWRRYISRYGVQGLYKRNVELLGFIEKKDVLSAKQLIA
                     DSVSATISGSTEIY"
     gene            complement(4981..>5246)
                     /locus_tag="PLR57_00525"
     CDS             complement(4981..>5246)
                     /locus_tag="PLR57_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39968.1"
                     /translation="LIATIYIANNDSQKAYQHILNFAKLCDYPISIYNFQNILPYFNG
                     SLQEVIDILQYRAKAAPEGQKRDSYFETIQNLEKREAKLLNQE"
BASE COUNT         1020 a         1492 c         1502 g         1232 t
ORIGIN      
        1 gtattcctac gataggggct tttatttgtg tctaatcaac ggggttcagt tcaaagcggg
       61 caaaaacaga tggcgcgggg ttacggctct tccggctcgt cctcctcata caccacaatc
      121 tctttaatat tgaactcctg acctgtcacc atgacgcatt cccatgcgcc gcacttgggg
      181 cagacgcggt tgtttttaag gtactcgtag acattgccgc attttttgca ctcgacttcg
      241 gcaacgatca cctcgacttc gagcgtcgcg ctttccattt ccgtgccgtc ggtggcggcg
      301 ggccagcact ccttgaggta gcgggggacg atggtggaag cctcgccgac ttccaccacc
      361 acgcttgcga tatcacgcag ctccagaccg ttttctaagg caacggcgga gacgcgtttt
      421 tgaatttcga atacgatacc cagttcgtgc atgctgctcc cgttactttg cgcccagcgc
      481 gcgcttgact ttcttgatgg caatcttgcc cttgcgcttg agaatctgct tgatgacgac
      541 cggcttcatc tccgtgaact ccacgcccgc gccgtcgtag cggcggacga ggtggatggc
      601 gtcgaacttg cactttgtgg tgcactgccc gcagccgacg cacatatacg aatcgaccag
      661 cgtgactccg cagccgaggc agcgcgcggt ttccttttgc acctgttcgg gcgtgaacgt
      721 gccgcgcagg tcgcggaagg tgcgggtcgc gggctccttg ctgtccagcg tttcgggctt
      781 ctggcgcggg gtatggtcgt aatcgtcgat caccgcgctc tttttgtcga gttcaacgta
      841 ttcgcggcgg tcgcgtccga tggtcagcga ctgtccggga tggacatagc ggtggatgga
      901 gatagcggcc tgcttgcccg cggcaatcgc gtcgatggcg aacttcggcc ccgtgcagca
      961 gtctccgccg gtgaacacgt ccgcttggcc ggactggtag gtaaagccgt ccgcctgcag
     1021 ggtgccgttg cggttgaact gtgcgtttgt tccctcaaga aggtcgcccc agaggatgct
     1081 ctggccgatg gtcacgagaa cgctgtccgc ttcgacaatg acggtgtccg cttcgtcata
     1141 cttgggggag aagcgtccgt ccgcgtcgaa cacgctgagg cactttttga actccacgcc
     1201 cgtgaccttg ccgttttcgg tgagaatccg tttggggcca aagcaggtgt gaatggagat
     1261 gccttcgcct tccgcttcct caatttcctc ggcggaggca ggcatgatgt cccgcgattc
     1321 gaggcagtac atggcgaccg tttccgcacc ggagcgaacc gccgttcgcg caacgtccac
     1381 agcgacgttg ccaccgccga tgacgatggt tttgccggag agcttgaggc tttctcccgc
     1441 gttgatcttg cgcaagaact cgatgccgga gagaacgcct ttcgcgtcct cgccttccac
     1501 gccgaccttg cgtccgccct gcgcgccgat ggcgacgtag aacgcgcggt aaccctgctt
     1561 acgcagcgct tccagtgtga tatcttttcc gacttccacg ccggtttgga actgcacgcc
     1621 catctggcgc aggatgtcga tttccgcttc cagaacaccc ttttccagtc ggaaggaagg
     1681 gatgccgagg gtcagcatgc cgccgagctt ttgctctttt tcaaacacgg taaccgtgta
     1741 gccgtccagc gcgaggtaat acgcgcagct taagcccgcc gggccggagc ccacgacggc
     1801 gattttatag tcggagtaat catgccgctt gctcggaaca tatctgcccg cgctggtgag
     1861 ttcctgagcc gcgatgaact ttttgatctc gtcgatggcg atggggtcgt ccagctcgcc
     1921 gcgtgtgcag gcctgctcgc agcggcggtt gcagacgcgc ccgcaaacgg cggggaaggg
     1981 gttttcccgc ttgatgagct ccaacgcttc cagatacttg ccctgggagg cgagcttgat
     2041 atagccctga atgccgatat gcgccgggca gttcgtcttg cagggggaag tgccgctatc
     2101 caccacgtcc tcgcggtttt cacggtagcc ggggttccat ttatccggcg tccagtcctc
     2161 gtcccgcggg gtaatcacgg tggtgatgtc gctgacgaca ggctttttcg agcagagctt
     2221 ctggccgagc ttgagggcgt tgacggggca gttttccacg cactgtccgc aggcgacgca
     2281 tttggaagca tccacttccg aaacgtagtt cgagcgcacc atgtccacgt ttttgaacat
     2341 ggccgccgtg cggagcgaaa ggcacccgca gccgcagcag ttgcagatgg cgtgcgtctt
     2401 gcccgggccg tcggtattcg gtatttcgtg catgaggccg ttttcttccg cgcggcgcag
     2461 aatttcgtac gcttcctcgc gggtgatctg tcggccgcgc ccggtgcgga tataatactc
     2521 cgcggcgtga cccatctgga tgcacatatc ctccttgagg tggccgcagc cttcgcccat
     2581 gacctcgcgg tcggtgcggc aggagcaggg ggaaacggag aagatggtgt tctcttccaa
     2641 ataggtagaa atttcttcgt agctcgcctt gcggctttcg ccgtcgatgg cgctctcaat
     2701 ggggatcacg cgcatgagac caacgccgac ggggaacgag ccggagctca tagggccgcg
     2761 tacgcgcccg taggcttcca tggcgtagcc gacgacgggg tattttttaa tgttgtcgag
     2821 gttgttgacg atcatctcca tcgtgccggg aatccaggtc tccgtccaga acatgtccac
     2881 gccgtcgatg cagttgacga agcacgcgcc gtagacggcg agcttcatga gctgctcatg
     2941 gcagaacgct tcgggcttgc cgacgatagc ggcgacttcc gcggcggact tcttttcgta
     3001 ggagccgagc gcaaggccaa caaggcacat gtcgtcgtca acacccgctt ccagaataac
     3061 gtattccggg tcgtcccatt tgagggagcc cttttcgccg ggtttaacgc cgggcttttt
     3121 gcgcccgatc atgttggcca gctgcatgat gttttcgcgg tactggcaga cgtacccttc
     3181 cgggaagatc atcttggtga ggttttccat gcgtcgcttc tccttatatt aaactcaaaa
     3241 tatggggttt ccaaaggggc gtatgcccct ttggcctgcg cggagcgcaa tcctaatctc
     3301 ttttccagtc cgcaacctgc ctgcgcagcc agtttgccca aacgtctatt ccctcgccgg
     3361 tgcgcgcgca gatggggata atctcgatat ccggattcag ctttttggcg cgctcggtca
     3421 aggccgccat atcaaaatcg aaatacggca gcacgtcgat cttgttgaca agcagcacgt
     3481 ggcaaatgga gaacatcagc gggtacttca gcggcttatc gtcgccttcc ggtatcgaga
     3541 ggatcatcgc gttcttgacg gaacccgtgt cgaactccgc ggggcagacg aggttgccca
     3601 cgttttcgag aaagacgagg tcaagcccgt ccacgccgaa ttcgtcgagt ccctgccggg
     3661 tcatgtccgc gtccagatgg cacatgccgc cggtgtgcag ctgaatggcg cgcacgccgg
     3721 tttccgcaat agtgcgggcg tccacgtccg aatcgatatc cgcttccatt acgccgatgg
     3781 cgagctcgtc cttgagcgct tctatggtgc gtttcagcgt ggtcgttttg cccgcgccgg
     3841 gggaactcat taagttgagc agaaaggttt tccgctgttt cagctcgctg cggagcttgt
     3901 ccgcctcgcg ctcgttgctc tcaaacacgc gctgcttgat ttcgtaaact ataaacatgt
     3961 ttgcctccaa gtccattttt tcatgcgctc tgtggtatga tatcaaatga aacattgctg
     4021 aatcgataca tccctcccgg cttcggaagg aaacgccaaa cagaacggat gggaaaacgg
     4081 gaatgtctct ggttgaacca catatcgata gcataacact gaatacgcac gcggtcaaga
     4141 tttgcccgca acagcgcgat tttattctgg aaaatatatt ttagaagtga taaaatcacg
     4201 gtgtaatctc tggcgcaacc aagtaccacg caaacaataa gcaaagaggc aggaaacaat
     4261 gttcaaaaaa ctgaccgcgc cgacgctcaa gcagctgttc gtgcaggaac tggaatccat
     4321 gattctctcg ggcgagctcg ccatcggcca gaagctcccg ccggagcgcg aacttgccga
     4381 gcggatgcag atcagccgct cggtggtcaa cgacggaata gccgagatgg cgcgcaaggg
     4441 ctttttggag atctcgccgc gccagggcac ggtggttgcg gactttcacc gcaacggcac
     4501 gctggagatt ctcgtttcca ttatgtccta caacggcggc cagctcccga gggacgaaat
     4561 ccgttccatt ctggaactgc gcggggtatt aatggcgttc gcgctggaaa gtgcgattcc
     4621 gctcattacg cccgagcagc tcgcccgcct gaacgaacag agcgaggcgt ttgtgaacgc
     4681 gccctcgccc gaggaggcgg cgacccgcat ctataccttt gaccatatgc tcagcggttt
     4741 ttccggcaac acgctgctgc cgctgatctt ctgctcgttc cgcgcgccca tcaaggagct
     4801 ctggcgtcgg tatatttcgc gctacggtgt gcagggctta tacaagcgca acgtagaact
     4861 gctcggcttt atcgaaaaaa aggatgtttt gtccgcaaaa cagctaatcg cggacagcgt
     4921 cagcgcgacc atcagcggca gcacggagat ctactgagga ttcccgattt ggatatttag
     4981 ttactcctgg ttgagcagct ttgcttctcg cttttcaaga ttctgaattg tttcaaaata
     5041 agaatctctc ttctggccct caggggcggc ctttgctcgg tattgaagta tatcaataac
     5101 ttcttgcaat gagccgttga aataaggcaa gatattttga aaattataaa tactgatagg
     5161 ataatcgcat aatttggcaa aattcaaaat atgttgataa gccttttgcg agtcattgtt
     5221 tgctatgtat attgtcgcaa ttaata
//
LOCUS       DAOMGY010000015         5205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_16183_length_5205_cov_3.033398, whole genome shotgun sequence.
ACCESSION   DAOMGY010000015 DAOMGY010000000
VERSION     DAOMGY010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16183_length_5205_cov_3.033398"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1163
                     /gene="rlmN"
                     /locus_tag="PLR57_00530"
     CDS             114..1163
                     /gene="rlmN"
                     /locus_tag="PLR57_00530"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQQ39969.1"
                     /translation="MYLADLTKDELVKLFAEWGEPKYRTAQAFEWLKKGARPEEMTNL
                     PLPLREKLAEIPYGGVTIEQKFLSQKDGTVKYLFRLEDENLVEGVLMRYHYGNTLCLS
                     TQVGCNMGCKFCASTLEGCVRNLRVGELLGQIHAVEQNEPTPEPGHRNVTNLVLMGSG
                     EPLDNYENVVAFLNFVTAQDGLRISARNISVSTCGIVPKIDSFIEDAPHVTLSISLHA
                     HDDETRTSLMPINRAYPISELLAASRRYAEKTGRRVVFEYALIGGVNASLADADALAS
                     LLRGMLCHVNLIPLNPVSERGLAGVSRAEAQAFCDRLNDKKISATVRREMGTDIDGAC
                     GQLRRRIMHEICGNN"
     gene            1141..1872
                     /locus_tag="PLR57_00535"
     CDS             1141..1872
                     /locus_tag="PLR57_00535"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HQQ39970.1"
                     /translation="MKYAAITDIGKRSHNEDSYLLPPEGCDICLFAVADGMGGHAAGA
                     VASKLLVEGLSGSPVAFETGREIEQLKSAIERANLSVYEIAEDDISLRGMGSTLVCTL
                     ILGDKFIAANVGDSRVYHFDGEVFSRVTIDHSLVEQLVLQGAITKKQARVHPQRNIIT
                     RAMGISPVVDVDLFERDWEPGDMLLLCSDGLHGSVEDEDIAEVLQSDLNLETKCELLV
                     RRALNNGGTDNITIILIQNDEEDAV"
     gene            1869..3746
                     /locus_tag="PLR57_00540"
     CDS             1869..3746
                     /locus_tag="PLR57_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ39971.1"
                     /translation="MIAPGTIVGHRYRIIDLIGSGGMAQVYRAMNLASRKIVAIKVLK
                     DEFRNDAEFLRRFEREARAVLHLSHENIVRAFDVGEAEGLPYIVLEYIDGRTLKEILD
                     ENGPMPPRIAVALVVQVLDALNAAHNAGIIHRDVKPQNVIVMQSGKVKLMDFGIAREV
                     DANTVTFTGSTVLGSVHYLSPEQAKGQPVTEGSDLYSAGIMLYEMLTGHVPFEGDNSV
                     AIALKHISDMPKPPADLNPKIPPALNDVVMRALNKDLNRRYQSANEMSAHLRRSLKEP
                     TGDFARFVATPKPTTDRKPPVKKKTHGALKIGLAVAAVLVVLVGAFIVLRSAFSSSED
                     TMEYVPTLVDRTIDEARQKAENFGFVFDIQDYENDDNVASGSVILQTPEAGIKAMRGT
                     TIYCVISLGPAAPNVPNLIGLTPDEAKRALEELGLELGSVSYRISETAIGYVCEQSVP
                     PDTEATSGQIIDIVISASSETTFAMPYAMDEPLNIILDQLRLASFNNIFVRYSASDTI
                     EDGVVFLQDPLPDTQVLPETPVTLTVCGDPVYDFEADIAFNIDIENSGTKVLVVIQDS
                     YNGLTYDRILYESTLEKGSKVPISFTAYSDIEGTREVVLLQDGKEIKRQDFTFTAKAP
                     Q"
     gene            3879..4754
                     /gene="rsgA"
                     /locus_tag="PLR57_00545"
     CDS             3879..4754
                     /gene="rsgA"
                     /locus_tag="PLR57_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQQ39972.1"
                     /translation="MEQGRIIKGVGGFYDVLTENGAVVTCKARGRFRNEHIAPMVGDL
                     VEISHPETGYAAIDELLPRRNALVRPSVANIDQLIIVAAAGAPKPDWLLIDKLLLQCH
                     ALKIEPLLVLNKIDIGEETVLSEFRRDYAAFPSLLVSSKTGEGIDLLKERLSNRISCF
                     AGQSAVGKSSLLNALFPALKLETGGLAKKTDRGRHTTRQAELWPLLGGAVLDTPGFSL
                     LEIEEFTQDQLNESWPEFQKAYERCRFSGCSHVSEPDCAVKELLAQGAVSPGRYERYK
                     EIRSEIEQRRKHRYD"
     gene            4747..>5205
                     /gene="rpe"
                     /locus_tag="PLR57_00550"
     CDS             4747..>5205
                     /gene="rpe"
                     /locus_tag="PLR57_00550"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007499663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQQ39973.1"
                     /translation="MIKIAPSILSADFANLGRDIERLSDWKADWIHFDVMDGHFVPNM
                     SFGPVLCSAIRPLTKLPIDVHLMVNEPSRFFDWFVKAGADIITFHAEADPHIHRSLQH
                     LHERGVKAGVVLNPGTPAVAVKEVLPYCDLVLVMSVNPGFGGQKFIPESLS"
BASE COUNT         1182 a         1454 c         1488 g         1081 t
ORIGIN      
        1 cattccgatt attctctcga acgcatgccg cttccgttgg aaaacgacgg catgctccag
       61 cttctgccgc agcggcacgg caccgacgga ttttttattg cgaggatgcg aaaatgtatt
      121 tagcggatct tacaaaagac gaactcgtca agctgtttgc cgagtggggc gaaccgaaat
      181 accgcaccgc gcaagccttc gagtggctca aaaaaggcgc gcgtcccgag gagatgacaa
      241 accttcccct gccgcttcgc gaaaagctag cggagattcc atacggcggc gttacgattg
      301 agcaaaagtt cctttcgcaa aaggacggaa cggtcaagta cctgtttcgt ctggaggatg
      361 aaaaccttgt ggaaggcgta ctgatgcgct atcattatgg taataccctc tgcctgtcta
      421 cgcaggttgg ctgcaatatg ggctgcaagt tctgcgcttc cacgctggaa gggtgcgtgc
      481 gaaacctccg cgtcggcgaa ctgcttgggc agattcacgc cgtcgagcag aacgaaccca
      541 cgcctgagcc cggacaccgg aacgtgacaa accttgtcct catgggcagc ggagagccgc
      601 tggataacta cgaaaacgta gtcgccttcc ttaactttgt tacggcgcag gacggccttc
      661 gcatcagcgc gcggaatatc tccgtttcca cctgcggcat cgtgccgaag atcgattcgt
      721 tcatcgagga cgcgccgcac gtaacgcttt ccatttcgct gcacgcgcac gacgacgaaa
      781 cgcggacgtc gcttatgccg atcaaccgcg cttacccgat ttccgaactg ctcgcagctt
      841 cgcgacgtta tgcggagaag actggacgca gggttgtgtt tgaatacgcg ctcatcggcg
      901 gcgtcaacgc ttcacttgcg gatgcggatg cgctggcaag cctgctgcgt ggtatgctct
      961 gccacgtgaa tctgattccg ctcaaccccg tatccgaacg agggctcgca ggcgtcagcc
     1021 gcgcggaagc gcaggcgttt tgcgaccgcc tgaacgataa aaagatttcc gcaaccgtgc
     1081 gccgggaaat gggcaccgat atcgacggcg cttgcggaca gctccgcagg aggattatgc
     1141 atgaaatatg cggcaataac tgacattgga aaacgctcac ataacgagga cagctatctg
     1201 ctgccgccgg aaggctgcga tatctgtctg tttgctgttg cggacggaat gggcgggcac
     1261 gcggcgggag ccgttgccag taaactgctt gtggaaggtc tctccggcag tcccgttgcg
     1321 tttgaaacgg gccgcgaaat tgagcagtta aaaagcgcga ttgaacgcgc aaacctgagc
     1381 gtttacgaaa ttgccgagga cgatatcagc ctgcgcggta tgggctccac gctcgtctgc
     1441 acgctgattc taggcgataa attcattgcc gccaatgttg gcgacagcag ggtttatcac
     1501 tttgacggcg aagtcttcag ccgcgtcacc attgaccatt cgcttgtgga acagctcgtt
     1561 ttgcagggcg cgatcacgaa aaagcaggcg cgtgtgcatc cgcagcgcaa tatcattacg
     1621 cgcgccatgg gcatttcccc cgttgtggac gtcgacctct ttgagcgcga ctgggaaccg
     1681 ggcgacatgc tgctgctttg ttcggacggg ctccacggtt ccgttgagga cgaagatatc
     1741 gcggaagttt tacagtccga cttgaatttg gaaaccaagt gcgagctgtt ggtgcggcgc
     1801 gcgctgaata acggaggaac ggacaacatt accatcatcc tgattcaaaa cgatgaggag
     1861 gatgcggtat gattgctccg ggtacgattg taggacatcg ttatcgaatt atcgacctga
     1921 tcggctccgg cggaatggcg caggtttacc gcgccatgaa cctcgccagc cgcaagattg
     1981 tcgccatcaa agtactcaag gacgagttcc gcaacgacgc ggagtttttg cgccggttcg
     2041 aacgggaagc gcgcgcggta ctgcatcttt cgcatgaaaa tatcgttcgt gcgttcgacg
     2101 ttggcgaagc cgaaggcctg ccgtatatcg ttttggagta tattgacggg cgcacgctca
     2161 aggaaatact ggatgaaaac ggcccaatgc cgccacgcat cgctgtagcg ctcgtggtgc
     2221 aggtgctcga cgcgctgaac gccgcccaca acgcgggcat tatccaccgg gacgtcaagc
     2281 cccagaacgt catcgttatg cagagcggca aagtcaagct catggacttt ggcattgcgc
     2341 gcgaagtgga cgcaaacacc gttaccttta cgggttcgac agtgctgggt tccgtacatt
     2401 acctttcccc cgagcaggca aagggccagc ccgttacgga gggaagcgac ttgtattccg
     2461 cgggcatcat gctctacgaa atgctgaccg ggcatgttcc gtttgagggc gacaactccg
     2521 ttgccatagc gctcaagcac atcagcgaca tgccgaagcc ccccgccgac ctcaacccga
     2581 agattccgcc cgcgctcaac gacgttgtga tgcgcgcgct gaataaggat ctcaaccgcc
     2641 gctatcagag cgcaaacgaa atgagcgcgc acctgcgccg ttcgctgaag gaaccaacag
     2701 gtgactttgc gcgctttgtt gccacgccga aaccgaccac cgaccggaaa ccgccggtga
     2761 agaagaaaac gcacggcgct ctgaagattg gccttgcggt cgcggcggtg ttggtcgtgc
     2821 tggtcggcgc gtttatcgtg ctccgcagtg cgttttcttc cagcgaggac acaatggaat
     2881 atgtgcccac actggttgac cgcaccattg acgaagcgcg gcagaaggcg gaaaacttcg
     2941 gcttcgtgtt cgatattcag gactacgaaa acgacgacaa cgtcgccagc ggaagcgtga
     3001 ttctgcaaac gccggaagca ggcatcaaag ccatgcgcgg aacgacgatc tactgcgtca
     3061 tcagccttgg gccggccgcg ccgaacgtac cgaacttaat cggcctgacg ccggacgaag
     3121 caaagcgcgc gcttgaagag cttgggctcg aacttggcag cgtaagctac cgcatttcgg
     3181 aaaccgcgat cggctatgtc tgcgagcagt ccgtcccgcc ggacacggaa gcgaccagcg
     3241 gacagatcat cgacatcgtt atcagcgcgt cttccgagac gacgtttgcc atgccttacg
     3301 ccatggacga gccgctgaac atcatactcg accaactgcg gctagcgtcg ttcaacaaca
     3361 tcttcgtgcg ttactccgct tcggatacca ttgaagacgg cgtagtcttt ttacaggatc
     3421 ccctgcccga tacgcaggtg ctgccggaaa cgcctgtcac ccttacggtg tgcggcgacc
     3481 cggtatacga ttttgaagcg gatatcgcgt tcaatatcga tattgaaaac agcggcacca
     3541 aggtactcgt cgtgattcag gattcctaca acggtttaac ttacgataga atcctctacg
     3601 agagcacact tgaaaaaggc agcaaggtgc ccatttcctt taccgcgtat tccgatattg
     3661 aaggcactcg cgaagtagtg cttttgcagg acggcaagga aattaagcgg caggacttta
     3721 cgtttacggc caaagcgccg cagtaacaca gcattgaaaa aagcggaaag aaatgaaaag
     3781 agcgcaacaa ttaaaaagag cgcaataatt aacagagcac aacaaaggag cctgattgat
     3841 ttgaaaaaac ttcctgtcac ggagaatgtg aaccgttgat ggagcaggga cgcattatca
     3901 aaggcgtcgg cgggttttac gacgtgctga cggagaatgg agcggtcgtc acctgcaagg
     3961 cgcgcgggcg attccgcaac gagcatattg cgcccatggt gggcgatctt gttgagattt
     4021 cgcatcccga aaccgggtat gccgccattg acgaactcct tccgcgcagg aacgcgctgg
     4081 ttcgcccgag cgttgccaac atcgaccaac tgatcatcgt tgccgcggcg ggcgcgccaa
     4141 agcccgactg gctgctgatt gataagcttc ttttgcagtg tcacgcgctg aagattgagc
     4201 cgctgctggt gctcaataaa atcgatatcg gcgaagaaac ggtgctttcg gaatttaggc
     4261 gcgattacgc cgcgttccct tcgctgctcg tctcgtcgaa gacgggcgaa ggaattgatt
     4321 tgctgaaaga acggctatcc aaccggattt cctgctttgc gggtcagtcc gccgttggaa
     4381 aatcctcgct tcttaacgcg ctcttccccg ctctcaagct ggaaaccggc ggccttgcca
     4441 aaaagaccga ccgcggcagg catacgaccc gtcaggcgga gctttggccg ctgctgggcg
     4501 gcgcagtgct cgacacgccg ggattttcac ttttggagat tgaggagttt acgcaggatc
     4561 agctcaacga aagctggccg gagtttcaaa aagcctatga gcgctgccgg ttttccggat
     4621 gctcccatgt ttcggaaccg gactgcgccg tcaaggagct gctcgcgcag ggtgccgttt
     4681 cacccggacg gtacgagcgg tataaagaga ttcgatcgga aatagaacaa aggagaaaac
     4741 accgttatga ttaagattgc gccttccatc ctttccgccg actttgccaa tttggggcgt
     4801 gatatcgagc gcctatcgga ctggaaagcc gactggatac actttgacgt gatggacggg
     4861 catttcgtac ccaatatgtc ctttggtccc gtgctctgct ccgccatccg cccgctgacg
     4921 aagctgccca tagacgtgca tctcatggta aacgagcctt cccgcttttt cgactggttt
     4981 gtcaaagcgg gcgcggatat catcaccttc cacgcggagg cggatccgca tatccaccgc
     5041 tccctgcagc atctgcacga gcgcggcgtg aaagcgggcg ttgtgctcaa ccccggcacg
     5101 ccagccgtcg cggtcaagga agtgctgccg tactgcgacc ttgtgctggt gatgtccgta
     5161 aaccccggtt tcggcgggca gaaattcatt cccgagtcgc tcagc
//
LOCUS       DAOMGY010000016         5193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_16230_length_5193_cov_3.435383, whole genome shotgun sequence.
ACCESSION   DAOMGY010000016 DAOMGY010000000
VERSION     DAOMGY010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16230_length_5193_cov_3.435383"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLR57_00555"
     CDS             complement(<1..692)
                     /locus_tag="PLR57_00555"
                     /inference="COORDINATES: protein motif:HMM:NF017321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQQ39974.1"
                     /translation="MFHNNIEESAPLADRMRPRTLDEFIGQEHIVGEGKLLRRAILTD
                     RLQSSIFFGPPGTGKTTLATIVANATGGAFVKLNAVTAGVKELRAIVEEAEQRLKLYR
                     QPTYLLLDECHRWSKTQSDSLLPALEKGTIRFIGSTTENPMIAMTSAIVSRCRVFQFY
                     PLTSEDVEQAVLHALHDEERGFGKLNVDLEDEALHTISAIANGDCRNALNALELAVLT
                     TPPDDAGVIHITR"
     gene            complement(765..1700)
                     /locus_tag="PLR57_00560"
     CDS             complement(765..1700)
                     /locus_tag="PLR57_00560"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HQQ39975.1"
                     /translation="MLRLLCLTPAQANGHTECDLTQNGSVNCSDACAALMCACGEISD
                     LDAFAERLSTGLLGEKYFDRFYYAGVYDDLNGNYKSANVSVSIISGRAYDNDYFLADI
                     YVQDIACLTNVFSQDEYMGDTETAAKLLNRCESGIVGINGDFYSLNLFGPLVRNGVKY
                     VKNITRALDIAILCKNGELLTYRYRILTADMLATLDVYQIWVFGPALLDDNGNTKTEF
                     RSHVTARNPRSVIGYYEPGHYAFLIVDGRREHSMGITMKDLSSLCKELGFTRAYNLDG
                     GQSSVLQSKSGAINIPFDGGRTLSDLFVVCDLPQE"
     gene            1993..3147
                     /locus_tag="PLR57_00565"
     CDS             1993..3147
                     /locus_tag="PLR57_00565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ39976.1"
                     /translation="MKIGQFSDTFLPIVDGVGRVVYNYAAALSRRGEECYVVAPMADG
                     GYRGGYPFELVDFISMELPRQKQYSTGIPQLDPHYDARMRCIDLDVIHAHSPFVSGLE
                     AQRLGRRRNVPVIGMFHSRYYDDFYQLTRAEMLANLAVATIVNFYLRCDEVWTVSQSS
                     AETLFDYGYNGEIVVMPNGTPDVSPDEVKCAAARAHFHLPEAPILLYCGQMNWKKNIL
                     RILESCACLKQKGESFSLVFAGQGPDAHAIQQKAAEFNLAERTIYTGHITDETLLYGL
                     FQCADLFVFPSLYDTSGMVVREAAAMETPSVVVRGSATAEPILDGQNGLLCEDTTESL
                     CAAIQRALSDPEATRQIGKNARKTIYVSWDDIAAHAVERYELLIDRFKSR"
     gene            complement(3252..3518)
                     /locus_tag="PLR57_00570"
     CDS             complement(3252..3518)
                     /locus_tag="PLR57_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HQQ39977.1"
                     /translation="MADKQPAGGNSAEAVLDLHGKNKYQARIAIEAALRRSKGLYRIR
                     VIHGCHSGTALKDMIWSEYGSDPRVKRLLAASENATDLVLRELY"
     gene            complement(3525..4814)
                     /locus_tag="PLR57_00575"
     CDS             complement(3525..4814)
                     /locus_tag="PLR57_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQQ39978.1"
                     /translation="MPNSKRTLQEQLHARKLSQPPKLIYRILGYLWKLLYFKKLNIHT
                     EIKNDPRKHKGPYIIVSNHASRLDYIYTGAVLLPDTLNYVAGYNEFFRSHLAGVFRLL
                     QVIPKKNFTPDIYTIKEIARILRRGGRIILFPEGMSSIGGGNQPCAIGSGKLLKHFGV
                     PVYISLISGGYLTSTKYCLDERPGRVEVVIDRLFTPEQLKELTAEEIQLRLDEAIHHD
                     DYEWNKTAQVAYAGNGKMAHQLHTLLYRCPRCGKEFTMRGEGNTIRCLACGNGATMDE
                     TYRMIPFDETCVIPDTPRKWFDEERKAIYRELIQPGFQLIERVKLGILPKDHFLKNQA
                     TSELVGEGEITLDSEGFAYTGTKHGEPFGFRLEPKLLPTYGMCTDVSRFYTFFEGEFF
                     EFFPETESVAKWLLATEELHRLSGGEWKNFPDAETYA"
BASE COUNT         1080 a         1484 c         1476 g         1153 t
ORIGIN      
        1 cgggtaatat gaatcacgcc cgcatcgtcc ggcggcgttg ttaaaaccgc gagctccagc
       61 gcgttgagcg cgttgcggca gtcgccgttg gcgatggcgg aaatcgtgtg cagcgcttcg
      121 tcttccagat cgacgttcag cttgccaaaa ccgcgctcct cgtcgtgaag cgcatgcagc
      181 accgcctgct cgacgtcctc actggtcagc gggtaaaact gaaacacgcg gcagcgcgag
      241 acgattgcgc tcgtcatggc gatcatgggg ttttccgtcg tggagccgat aaagcggatc
      301 gtgccttttt ccagcgcggg caggagcgaa tccgactgag ttttactcca gcggtggcac
      361 tcgtcgagca gcagatacgt cggctggcgg tagagcttga ggcgctgttc ggcttcctcc
      421 acgatggcgc gcagctcctt gacgcccgcg gtgaccgcgt tgagcttgac gaacgcgccg
      481 ccggttgcgt tggccacgat ggtggcaagc gtagttttgc ccgtgcccgg cgggccaaag
      541 aagatggagc tctggagccg gtcggtcaaa atggcgcggc gaagcagctt gccctcgccc
      601 acgatatgtt cctgcccgat gaactcgtcc agcgtacgcg ggcgcatccg gtctgcaagc
      661 ggcgcgctct cttctatatt gttatgaaac aaatcttcca ttttgctgtt gttttgtgct
      721 tcctttagtt cagtaatctt tctgccggtg ccgccggctc gccgttactc ctgcggaagg
      781 tcgcaaacga cgaacaggtc gctcagcgta cgtccgccgt cgaacggaat gttgattgca
      841 cccgacttgc tctgcagaac gctggactgc ccgccgtcga ggttataggc gcgcgtaaaa
      901 cccagctctt tgcaaagcga ggaaaggtcc ttcatcgtta tacccatcga gtgttcccgc
      961 ctgccgtcca cgattaaaaa tgcgtagtgc cccggttcgt aatacccgat aacggagcgt
     1021 gggtttctcg ccgtaacatg gctgcgaaac tcggtttttg tgttgccgtt gtcgtcgagc
     1081 agcgcggggc cgaacaccca tatttgatac acgtccagcg tggcaagcat gtccgccgtc
     1141 aggatgcggt agcggtacgt aagcagttcg ccgtttttgc aaagtatcgc aatatccagc
     1201 gcgcgcgtaa tgttttttac gtatttcaca ccgtttcgaa ccaacgggcc gaagaggttg
     1261 agcgagtaga agtccccgtt gatgcccacg attccgctct cgcatcggtt gagcagtttt
     1321 gccgccgttt cggtatcgcc catgtattca tcctgtgaaa atacgttggt gagacaggcg
     1381 atatcctgca catagatatc cgcaaggaaa tagtcgttgt cgtacgcccg cccgctgata
     1441 atggagaccg agacgttcgc gcttttatag ttgccgttta gatcgtcata cacgcccgca
     1501 taataaaagc ggtcgaaata cttctctccc agcaggccgg tagaaagccg ttcggcaaac
     1561 gcgtccaaat ctgaaatctc accgcaggca cacatcaagg ctgcgcaggc gtcgctgcag
     1621 ttcacgctgc cgttttgcgt taaatcgcat tccgtatggc cgtttgcctg agcgggcgtc
     1681 aggcacaaaa gacgaagcat tcgcgccgcg tctgcggcgg tcggtgaacc atttcccaac
     1741 agatcggcgg actctgcccc gtcgtcggca agagccccgc aaaacggcgc gcagaaaacc
     1801 agacaaaggg ctatcaggaa tgctgccagc cgtttcatta cgacactctc ccgatcatat
     1861 tgtgaaaagt ttgtttcatc ataccacgtt ttcactggtt atgatagaat gtccctgttc
     1921 caaacaagtt tactaccgaa tgctgaaaat aaaaccgagt gtcgaaatca tcgttttgag
     1981 gtgaaaatac gaatgaaaat agggcagttc agcgatacgt ttctcccgat tgtggacggc
     2041 gtcgggcgcg tggtctataa ctacgcagcg gcgttgtccc ggcgaggcga ggaatgctat
     2101 gtcgtagcgc ccatggcgga cggcgggtac cggggcggtt atccgtttga gctggtggat
     2161 tttatcagca tggagctgcc gcgccagaag cagtacagca caggaattcc gcagttggac
     2221 ccgcattacg acgcgcgcat gcgctgcatc gacctggacg tcatccacgc gcactcgccg
     2281 ttcgtttccg ggctggaagc gcaacgtctg gggcgcaggc gcaacgtgcc ggtgatcggc
     2341 atgttccact cgcgctatta cgacgacttt taccagctga cccgcgcgga gatgctggca
     2401 aacctcgccg tcgccaccat cgtgaacttc tacctccgct gcgacgaagt atggaccgtg
     2461 agccagagct cggcggaaac actctttgac tacggctaca acggcgagat cgtcgttatg
     2521 ccgaacggca cgccggacgt ttcccccgat gaagtaaaat gcgccgcggc aagggcgcat
     2581 tttcatctgc cggaagcgcc gattctgctc tactgcggcc agatgaactg gaaaaagaac
     2641 atcctgcgca tcctcgaaag ctgcgcctgc ctcaaacaga aaggcgaatc gttttcgctc
     2701 gtgtttgcag gtcaggggcc ggacgcgcac gccatccagc agaaggccgc ggagttcaac
     2761 cttgccgagc ggacgatcta taccgggcat atcacggacg aaacgctgct ctacgggctg
     2821 tttcagtgcg cggatctgtt cgtttttcct tcgctttacg atacctccgg catggtggtg
     2881 cgggaagcgg cggcaatgga gacgcccagt gttgtcgtac gcggcagcgc caccgccgaa
     2941 ccgattctcg acggtcaaaa cgggctgctc tgcgaggata cgaccgagag tctctgcgcg
     3001 gcgatacagc gcgccctttc cgacccggag gcaacccggc agatcggcaa aaacgccaga
     3061 aagacgattt acgtctcctg ggacgatatc gccgcccacg cggtagagcg ttacgagctg
     3121 ctgatcgatc ggtttaagag cagataaccg gcaggttctc agggctgcca aagccctgag
     3181 cgggagctcg agggcagcgc cctcgaaagc cgaaaaccaa acgcgaggcg gtttgcctcg
     3241 cgtttttgtt tttaatacaa ctcccgtaaa accaaatccg tcgcgttctc gcttgccgcg
     3301 agcaggcgct tgacgcgcgg atcgcttccg tactcgctcc agatcatgtc cttcagcgcg
     3361 gtgccgctgt ggcagccgtg gatgacgcgg atgcggtaaa gccccttact ccgccgaagc
     3421 gcggcttcta tggcaatacg cgcctgatat ttgtttttcc cgtgtaaatc gagaacggcc
     3481 tccgcgctgt ttccgccggc cggttgctta tccgccatgt gcgcttacgc gtaggtttcc
     3541 gcgtcgggga agttcttcca ttcgccgccg ctgagacggt gcagttcctc ggtggcaagc
     3601 agccacttgg caacgctctc ggtttcgggg aaaaactcaa aaaactcacc ttcaaagaac
     3661 gtatagaacc gtgaaacgtc cgtgcacatg ccgtaagtcg gcaggagctt cggctccaga
     3721 cggaacccga acggctctcc atgcttcgtt ccagtatagg caaacccttc actgtccagc
     3781 gtaatttccc cttcgcccac gagttcgctg gtcgcctgat tcttgaggaa atgatccttg
     3841 ggcagaatgc cgagcttgac gcgttcaatc agctggaacc cgggctgaat cagctcgcgg
     3901 tagatcgcct tgcgctcttc gtcgaaccac ttgcggggcg tatcgggaat gacgcaggtt
     3961 tcgtcaaacg gaatcatacg gtaggtttcg tccatcgtgg cgccgttgcc gcaggccagg
     4021 cagcgaatgg tgtttccctc gccgcgcatg gtgaactcct tgccgcagcg cgggcagcga
     4081 tagagcaggg tgtgcagttg gtgcgccatc ttgccgttgc cggcatacgc aacctgagcg
     4141 gtcttgttcc actcgtagtc gtcgtgatga atcgcttcgt ccagacggag ctgaatctct
     4201 tccgcggtaa gctccttgag ctgttccggc gtaaagagcc ggtcaatgac cacttccacg
     4261 cggccgggac gctcgtccaa acagtatttc gtgcttgtca agtacccgcc ggagatcagc
     4321 gaaatataca caggcacgcc gaagtgcttg agcagttttc cgctgccgat ggcgcagggc
     4381 tggttgccgc cgccaatgga gctcatgccc tcgggaaaca ggataatcct gccgccgcgc
     4441 cgcaggatgc gcgcaatctc cttaatggta tagatatccg gcgtaaagtt ctttttcgga
     4501 atgacctgca aaagccggaa gacgcccgcc agatgcgaac ggaaaaattc gttgtatccg
     4561 gcaacatagt tcagcgtgtc cggcagcagt acggcgccgg tatagatata atccaaacgc
     4621 gaagcatggt tgctgacaat aatgtacggc cccttgtgct ttcgcgggtc gtttttaatt
     4681 tccgtatgga tattgagctt tttaaagtac agcagtttcc agagatagcc gagaatgcgg
     4741 tagatcagtt tgggcggctg actgagtttt cgcgcgtgga gctgctcctg cagcgtacgc
     4801 ttgctgtttg gcatatcgtt tcgctccccc aatcccaaaa aaacgttcta tgctgtgcat
     4861 tttagcaaat catactccaa cgcgcaaccg cagaaaattg cgaattgcat ataaaatagt
     4921 gctttagact caacgggttt ccagagaaca cgtcatccga ccggcagaac aagaaacgac
     4981 ggcgcgatta atgcgccgcc gtgctgtttc cgttctttgg ttcaaatctt ttcgaggctc
     5041 tcgttgtcgc ccagaatata gatggagtcg tccgcatgga acggttcgct ggggtctatg
     5101 tttcctgcaa cgtgcccgtc ccgccggatg gcgatgaccg agaagccgta tttgttgcgc
     5161 acgtcgatct gcgccagcga acgcccgatc cag
//
LOCUS       DAOMGY010000017         5151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_16385_length_5151_cov_3.124804, whole genome shotgun sequence.
ACCESSION   DAOMGY010000017 DAOMGY010000000
VERSION     DAOMGY010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16385_length_5151_cov_3.124804"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PLR57_00580"
     CDS             <1..803
                     /locus_tag="PLR57_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ39979.1"
                     /translation="GEPLLLIHSIGQSLFTWRNVFAELSDNYRVIALDLPGHGYSSRP
                     DTFTYTADDMAFVLQGFLDALSVKSAHMIGFSLGAIYILRFLSLYPKRVANCVAISPG
                     GITEQMPQLIHNMQKPLVSVFARNLYTASNVRKLLEECVTDPQLIDDHVVKQYYEPVS
                     DGLAREAIMYALRNFDLEAVAEGLLPVEHEVLVLWGKNDRWHPPSGSVYFQSLLRAGR
                     YYLVRNAGHLLQEEAPTKLLEIVYSYIPPAVPSYDVYRYTQHLAEDEE"
     gene            800..1006
                     /locus_tag="PLR57_00585"
     CDS             800..1006
                     /locus_tag="PLR57_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003682416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HQQ39980.1"
                     /translation="MIAEFGLGDVVMMKKPHACGVNEWVIIRVGADVKIKCSHCGRIV
                     MMDRADFVRMGKKIISKREQPSEA"
     gene            1068..1607
                     /gene="lepB"
                     /locus_tag="PLR57_00590"
     CDS             1068..1607
                     /gene="lepB"
                     /locus_tag="PLR57_00590"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQQ39981.1"
                     /translation="MRGSRAYRNVEFFIYILIVVLTALSIRSFLAEPIRVDGDSMIPT
                     LINNEDMLVEKVSYWFQDPTRGDMVICFYPGYTESCVKRVIGLPGETVSITDGLIYID
                     GTALDESDYWTGEIIGNMDPVTVGAREVFVVGDNRNGSKDSRNPYVGCIPYSKVVGRV
                     IAVVWPFSTFRTFERVNYS"
     gene            1604..2158
                     /gene="lepB"
                     /locus_tag="PLR57_00595"
     CDS             1604..2158
                     /gene="lepB"
                     /locus_tag="PLR57_00595"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQQ39982.1"
                     /translation="MTNGKRRLRSEGREQTYRNARRLGWLFSALVALFALCVVFFIWL
                     VPLRVMDASMSPALESGEVVLCDRLAKFVSAPERGDIILFQTEDGVFVKRIVGLPGES
                     VELVDGHVFINSRPLDESAYVTVYVGDMAPLTVPEGSLFVLGDNREQMYDSRLESVGC
                     IPFDQIEGMLRIRVSPIEKFTFFS"
     gene            complement(2311..3252)
                     /gene="arcC"
                     /locus_tag="PLR57_00600"
     CDS             complement(2311..3252)
                     /gene="arcC"
                     /locus_tag="PLR57_00600"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HQQ39983.1"
                     /translation="MEKLVIALGGNALQEGNGPATAEAQLAVVERTSVFIADIIEKGY
                     QVILAHGNGPQVGRIVLQNENSVSVTPAMPFDVCGAMSQGMIGYHMQQGLAKALRARG
                     NHTGVATVVTQVVVDQNDKAFLNPSKPIGPFYSEEEAKRLQAEKGYAVKEDAGRGWRR
                     VVASPVPVEIVELPEVECLIKGGFVVITVGGGGIPVLKDENGDLVGTAAVIDKDLASE
                     RLAEDVSADALLILTAVEQVCLNYGKPEEKRLSTITVAEAEQYIAEKHFAPGSMLPKI
                     QATVLFAKSKPGRRAIITSLDKAVDALHGTAGTTITL"
     gene            3708..4988
                     /locus_tag="PLR57_00605"
     CDS             3708..4988
                     /locus_tag="PLR57_00605"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQQ39984.1"
                     /translation="MQKQYYEIIGGKRLEGTVTISGAKNAAVAIIPAAILAGETCVLE
                     NLPHIQDVQSLKEILEELGVSVTLTKGDCMSIDSRTLTTTKALSEAVSSMRASYYLLG
                     ALLSRYGEAEVALPGGCVIGARPIDQHLKGMRALGAEIECDDDCNIVRAKAKKLVGAE
                     IYFDVVSVGATINVMLAACMAEGMTVLSNVAKEPHVVDVANFLNMMGASVKGAGTDVI
                     RIQGRPKLHGCGYSIIPDQIETGTFMIAAAATMGDVIIKNVIPTHMEAVSAKLMESGA
                     RVIEGDDGRDFYIRVIMNDRPRAINIKTLPYPGFPTDLQQPVMAYLCSSKGLSTINET
                     IFENRFNHVRELRRMGASISVKDSYAKVKGIASLHGADIYASDLRAGASLMVAGLAAN
                     GVTNVHNIQFIDRGYEYIDHKLNALGAKVTRKEK"
BASE COUNT         1141 a         1403 c         1455 g         1152 t
ORIGIN      
        1 tcggggagcc tctgcttttg attcactcga tcggtcagtc gttgtttacc tggcggaatg
       61 tctttgcgga actttcggat aactatcgcg tcattgctct cgacctgccc gggcacgggt
      121 actcctcgcg gcctgacacg tttacgtata ctgcggacga tatggcgttc gttttacagg
      181 gatttttgga cgcgctgagc gtgaaatccg cgcacatgat cggcttttca ctgggcgcga
      241 tctacatact ccgcttttta tcgctctatc ccaaacgcgt tgccaactgc gttgccattt
      301 cccccggcgg aatcaccgag cagatgccgc agctcatcca caacatgcaa aaaccgctgg
      361 tttctgtgtt tgcgcggaac ctctataccg cgagcaacgt tcgaaaactg ctggaggagt
      421 gcgtcaccga cccgcagctg atcgacgacc atgtcgtcaa gcagtattac gaaccggttt
      481 ctgacgggct tgcgcgcgaa gcgattatgt acgcgctgcg caattttgat ctggaagcag
      541 tagcggaagg gcttttgccg gttgagcacg aggtgcttgt gctctggggc aagaacgacc
      601 gctggcatcc gccgtcgggc agcgtgtact ttcagagcct gctgcgcgcg ggcaggtact
      661 atctcgtgag aaacgcaggc catcttctgc aggaagaagc gccgaccaaa ctgctggaaa
      721 tcgtctacag ctacattccg ccggcggtgc cgagctacga tgtataccgg tatacccagc
      781 atttggcgga agacgaagaa tgatagcgga attcggcctt ggcgatgtgg tcatgatgaa
      841 aaagccgcac gcctgcggcg tcaacgaatg ggtcatcatc cgcgtcggtg cggacgtcaa
      901 gatcaaatgc agccactgcg ggcgcattgt gatgatggac cgcgcggact ttgtacgcat
      961 gggcaagaaa attatatcga aacgggaaca gccaagtgaa gcgtagaacg tttacgccag
     1021 accagattaa gtatctgtgc gaccgcgcgg aagaacaata tgaaaatatg cgaggctcgc
     1081 gtgcgtatcg caacgttgag ttttttattt atattctgat cgttgtgctc accgcgcttt
     1141 ccatccgctc gttccttgcc gaacccatcc gcgtggacgg cgattccatg attccgacgc
     1201 tgatcaacaa tgaggacatg ctggtcgaaa aggtcagcta ctggtttcag gatccgacgc
     1261 gcggggatat ggtcatttgc ttttacccgg ggtataccga aagctgcgtc aagcgcgtga
     1321 tcggcctgcc cggagaaacg gtctccatta ccgacggatt gatctatatc gacggaacgg
     1381 cgctggacga aagcgactac tggaccggcg aaatcattgg aaacatggat cccgtaacgg
     1441 tcggcgcgcg ggaagtgttt gttgttggcg acaaccgcaa cggcagcaag gacagccgca
     1501 acccctacgt gggatgcatt ccgtactcca aagtagtcgg ccgtgtgatc gcggtcgtat
     1561 ggccgtttag cacgttccgc acgttcgagc gggtgaacta ctcgtgacga acggtaagag
     1621 gcgccttcgc agcgaaggcc gcgaacaaac ctaccgcaac gctcgaaggc tcggctggct
     1681 tttctccgcg ctcgtggcgc tctttgcgct ctgcgtggtt ttttttatct ggctggttcc
     1741 cttacgcgtg atggacgcga gcatgtcccc cgcgctggaa agcggcgaag tcgtgctctg
     1801 cgaccggctg gcaaagttcg tctctgcgcc cgaaaggggc gatatcatcc tgtttcaaac
     1861 ggaagacggg gtttttgtca agcgtatcgt cggcctgcct ggcgagagcg tggagctggt
     1921 tgatgggcat gtatttatca acagccgtcc gctcgatgaa agcgcgtatg tgaccgtcta
     1981 cgtgggcgat atggcgccgc tgaccgtgcc ggaggggtct ttgttcgttc tgggcgacaa
     2041 ccgcgagcag atgtacgaca gccgccttga gagcgtgggc tgcattccgt tcgatcagat
     2101 cgaaggcatg ctccgaattc gcgtttctcc gatcgagaag tttactttct tttcttaact
     2161 tcttttcttg aaagaaaaga agcaaaagca acgatctctt cttcttttct taaaagaaaa
     2221 gaagcaaaag aatttactcc tgatatacaa agagcttccc tataagagga aagctctttt
     2281 tgtttctttt tctctcgaga aaaagaaaca ttacagcgta atcgtcgttc ccgcggttcc
     2341 gtggagtgcg tcgacggcct tgtcgagcga cgtgatgatg gctctgcggc cgggcttgct
     2401 cttggcaaag agcacagtag cctgaatctt gggcagcatg gagccgggcg caaaatgctt
     2461 ttcggcaata tactgctccg cttcggcaac agtgatggtg ctgaggcgct tttcttccgg
     2521 tttgccgtag ttcaggcaaa cctgctcgac ggcggtaagg atgagcagcg cgtcggcgga
     2581 aacgtcctcg gcgaggcgct ctgaagcgag gtccttgtcg atgaccgccg cggtgccgac
     2641 gaggtccccg ttttcgtcct tgaggacggg aatgccgccg ccgccgacgg tgatgaccac
     2701 gaacccgcct ttgatgaggc actcgacctc aggcagctct acgatttcta ccgggacggg
     2761 ggaggcgacg acgcggcgcc agccgcggcc cgcgtcttct ttgacggcgt aacccttttc
     2821 ggcctgcagg cgtttggctt cctcttcgga atagaacggc ccgatgggct tggaggggtt
     2881 taaaaacgct ttatcgttct gatcgaccac aacctgcgtg accacggtgg caacgccggt
     2941 gtggttgccg cgcgcgcgga gcgccttggc gagcccctgc tgcatatggt agccaatcat
     3001 gccctggctc attgcgccgc atacgtcgaa cggcatggcg ggggtaacgc tgacggagtt
     3061 ttcgttctgc agcacgatgc gtcccacctg cgggccgttg ccgtgcgcaa ggataacctg
     3121 atagcctttt tcaatgatgt ccgcaatgaa aacggacgtg cgttccacga ccgcaagctg
     3181 cgcttcggcg gtggcggggc cgttgccctc ctgcagcgcg tttccgccca gggcgatgac
     3241 caatttttcc atggggctcc tccgcaatct taaaaaaata aagtcttgca aaaattactt
     3301 tagcatgcag tttgccgctt gtcaattcat tcttatccat atatgcgtaa aattgaaaac
     3361 tttcacgaaa ttgcattctc aacgaggcgg tattgcatca aaaaagatgc tttcctgcac
     3421 gaatgtgtaa aaaggtgcct gaaaaaccgc ggtatcatat atacttgcgc gctatcgtaa
     3481 agttaatgcc gatcatgata gatcatataa ttctgcaggc tgctgcgcgc gcgcttgcag
     3541 aaaaacactt attgagaaac gcaaaactgt ccaatgcaga gctcataaag gcggcgttcg
     3601 cgggttttat gcagcttgag aacgggtgaa ccgccgaaaa cgagcgtttt caggcttatt
     3661 tgccgccgct tgactttgaa agtggattcc gctagactgg tcatggtatg caaaagcaat
     3721 attatgaaat cattggcggg aaacggctcg aaggaaccgt aaccatcagc ggcgcgaaaa
     3781 acgcggccgt cgccatcata ccggctgcaa ttctcgcggg cgaaacttgc gtcctcgaga
     3841 acctgccgca tattcaggac gtccaaagcc tcaaggaaat ccttgaggag ctgggcgttt
     3901 ccgttaccct gaccaagggc gactgcatga gcatcgattc gcgcacgctg accaccacca
     3961 aggcgctcag cgaggcggtc agcagcatgc gcgcttctta ctatctgctg ggcgcgctgc
     4021 tttcgcggta tggcgaggcg gaagtcgcct tgcccggcgg ctgcgtcatc ggcgcgcgtc
     4081 ctatcgacca gcacctcaaa ggcatgcgcg cgctcggcgc ggagatcgag tgcgacgacg
     4141 actgcaacat cgtccgcgcc aaagcaaaaa agctcgtcgg cgcggagatc tattttgacg
     4201 tggtcagcgt cggcgcaacc atcaacgtca tgctcgcggc ctgcatggcc gaggggatga
     4261 ccgtgctcag caacgttgca aaggaaccgc acgtagttga cgttgcgaac ttcctcaaca
     4321 tgatgggcgc gagcgtcaaa ggcgcaggca cggacgtcat ccgcattcag ggccgcccga
     4381 aactgcacgg ctgcgggtat tccatcattc ctgaccaaat tgaaaccgga acctttatga
     4441 tcgccgccgc cgccacgatg ggcgacgtga tcattaaaaa cgtaatcccc acccatatgg
     4501 aagccgtctc cgccaagctc atggagagcg gcgcgcgcgt catcgaaggc gacgacggcc
     4561 gggacttcta catccgcgtt atcatgaacg accgtcctcg cgccatcaac atcaaaaccc
     4621 tgccgtatcc cggctttccc accgacctgc agcagccggt catggcttac ctctgctcct
     4681 ctaaggggct ttccaccatc aacgagacga tcttcgaaaa ccgcttcaat catgtgcgcg
     4741 aactgcgccg catgggcgca tccatcagcg taaaggacag ctacgccaag gtgaagggaa
     4801 tcgcttccct gcacggggcg gatatctacg cgagcgatct tcgcgccggc gcttccctga
     4861 tggtcgcggg gcttgccgcc aacggcgtga ccaacgtcca caacattcag tttatcgacc
     4921 gcgggtacga atacatcgac cacaagctca acgcgcttgg cgcaaaagtg acgagaaaag
     4981 agaagtaggg cgggggagta cgtttctttt cttgaaagaa aagaaacaaa agaacttact
     5041 gatttagaat cattcgggct tatcgtgaga taagctcttt tattatattg ggttcgattc
     5101 actgagaaga gaaaagctct ttttgtttct ttttctctcg agaaaaagaa a
//
LOCUS       DAOMGY010000018         5083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_16684_length_5083_cov_3.451671, whole genome shotgun sequence.
ACCESSION   DAOMGY010000018 DAOMGY010000000
VERSION     DAOMGY010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16684_length_5083_cov_3.451671"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLR57_00610"
     CDS             complement(<1..195)
                     /locus_tag="PLR57_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39985.1"
                     /translation="MQKTAKNSSSNAKSFFTAGFTCACAAASSILVIRSLNVKTLSVF
                     LVSVAVLLISLIIIRLGLRPL"
     gene            598..1809
                     /locus_tag="PLR57_00615"
     CDS             598..1809
                     /locus_tag="PLR57_00615"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HQQ39986.1"
                     /translation="MRSPIQVFSEVGRLKKVLLHRPGQELENLMPEYLGRQLFEDIPY
                     LVHAREEHDQFAKTLRACGVEVCYLLDLTAEAVREAGVQERLVDDFVAEMGISAHGVE
                     DAVRAYLLDMPVPQMLSAMVRGVRKSELGMREHKHLVDYIDDAYPFYVDPMPNLYFTR
                     DPFFMVGDGVCISSMANVVRARETLFGCCLFQNHPVYKNTPMRHTRDDPFSIEGGDVL
                     VLSKEVIAVGISQRTDPNAVEALADRLIGEETGITRVIAIDIPKVRSYMHLDTVMTMV
                     DWDKFTIHPSILPMLRAFSLTKKQGKVTVSQEECDFRETLMDALHLDRVDLIACGGGS
                     AIDAAREQWNDGTNTLAVSPGEVIAFSRNYVTNGILRDHGVIVHEIPSAELSRGRGGP
                     RCMSMPLWREE"
     gene            1868..2850
                     /gene="argF"
                     /locus_tag="PLR57_00620"
                     /pseudo
     CDS             1868..2850
                     /gene="argF"
                     /locus_tag="PLR57_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361145.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
     gene            3050..4378
                     /locus_tag="PLR57_00625"
     CDS             3050..4378
                     /locus_tag="PLR57_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HQQ39987.1"
                     /translation="MEERYQNNVAVQVPRVLLPKRGTNYHKWAVVACDQFTSQPEYWE
                     EAKRIVGKAPSTLELILPEAYLEKPGEKERIAAIKASMRAYLDEGILEEKPQGFVLVT
                     RTVEGNTRTGLVMALDLEAYDYQKGSTTPIRATEGTIVSRIPPRLKIREGAPIELPHI
                     LVLIDDPDRTVIEPLALKKAKLQKLYDTDLMQDGGHITGHLVDAEDDILGVLSALEQL
                     KDAEKFRAKYGDHAPLLFAMGDGNHSFATAKAYWEKVKETLPESERADHPARFALVEV
                     ENVHDEGIVFEPIHRVVFGVSGNRAIETLLRFFKEQNGCARVELGRMPCAQSGDTHIL
                     PFLTGEVEGAFIVSCPKTQLAVGTLQAAIDALLQEVAGTEVDYIHGAEVVKELSKKGG
                     AIGFLLPAMKKSEFFPTVVYDGALPRKTFSMGEANEKRYYMECRRIEKKK"
     gene            4531..>5083
                     /locus_tag="PLR57_00630"
     CDS             4531..>5083
                     /locus_tag="PLR57_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQQ39988.1"
                     /translation="MAVKVVKFGGTSLCNAENFTKVKNIILADKARKYVVPSAPGKRF
                     PGDEKITDLLYLCYAQNSGGLSFDETFTQITERYVAIETELGLKTNIAAELAAIKKNI
                     ISGASEDYIASRGEYLSGLLLADFLGFGFLDAAELVSFHSDGTYDDETTQKWTQKLTD
                     TERVVVPGFYGRKRDGSIKTFSRG"
BASE COUNT         1231 a         1397 c         1488 g          967 t
ORIGIN      
        1 caagggacgc agtcccagtc gaatgataat aaggctaatc agcagtacag cgacagacac
       61 tagaaaaacg ctcaacgtct tcacgttgag cgagcgaatg accagtatgg aagaggcggc
      121 cgcgcatgcg caagtaaaac cagccgtaaa aaacgactta gcgttcgaac tgctgttttt
      181 cgcggttttt tgcatcacag ggcggctcct ttcatataga atatatgata ttggggataa
      241 tctagcatca atcaccctcc ttgtcaagca aaaatcatat attttggcaa atttcttttc
      301 ttctgcggct atgctgatac aataggattt gtggaaattc tgcatatttc ttttcgatgc
      361 agaaagttat acattgccgt ttccgaatcg accggatgcc ggaacgctgt ataatttgcc
      421 tgcgcgaaaa ctgttatgca cccgcgtgga gccgcaagcc gtggatgaac cacgatagcg
      481 aacgccgctc taaaaacgag tatgcagtcc gctgcgcccg ccgtaaaaaa cgaataaaaa
      541 caggaaccaa cttctgtttc ttcctaatta aaagaagagc cgaaaaggag aagatgcatg
      601 cgctcaccca ttcaagtttt cagtgaggtc ggccgcctca aaaaggtgct gctgcaccgc
      661 cccggtcagg aactggaaaa cctgatgccg gagtatctgg gacggcagct gttcgaggac
      721 attccctacc ttgtgcacgc gcgcgaagag cacgaccagt ttgccaagac gctccgcgcc
      781 tgcggagtgg aagtctgcta cctgctcgac ctgacggcgg aagccgtgcg ggaagccggc
      841 gtgcaggaac gcctcgtgga cgactttgtt gcggagatgg gtatctccgc ccacggcgtg
      901 gaggacgcgg tgcgcgcata cctattagat atgcccgtgc cgcagatgct ttcggcaatg
      961 gtgcgcggcg ttcgcaaaag cgagctcggc atgcgggagc ataagcacct cgtggactat
     1021 atcgacgatg cgtatccgtt ttatgtcgat cccatgccga acctgtattt cacacgtgac
     1081 ccgttcttta tggtgggaga cggcgtttgc atctccagca tggcaaacgt tgtgcgcgcg
     1141 cgggaaacgc tgtttggttg ctgcctgttc cagaatcacc ccgtgtacaa aaacacgccc
     1201 atgcgccata cgcgggacga cccgttttcc attgaaggcg gggacgtgct agtgctctcc
     1261 aaggaagtca tcgccgtcgg catttcgcag cgcaccgacc caaacgccgt ggaagcgctg
     1321 gccgaccgcc tgatcggcga ggaaacgggc attacccgcg tcatcgccat cgatatcccc
     1381 aaggtgcgct cctatatgca cctcgatacg gtcatgacca tggtggactg ggacaagttt
     1441 acgattcatc cttcgattct gccgatgctc agggcgtttt cgctgacgaa aaagcaaggc
     1501 aaggtaaccg tttcgcagga ggaatgcgat tttcgcgaaa cgctgatgga cgcgctgcac
     1561 ctcgaccgtg tcgatttgat cgcctgcggc ggcggcagcg ccatagacgc ggcgcgcgag
     1621 cagtggaacg acggcaccaa cacgctggcc gtcagccccg gcgaggtcat tgccttttcg
     1681 cgcaactacg tgaccaacgg catcctgcgc gaccacgggg taatcgtcca cgaaatcccc
     1741 tcggcggagc tctcccgcgg acgcgggggc ccgcgttgta tgagtatgcc actctggcgg
     1801 gaagaataag gggatattaa ggggctttgc cccttaagac cccgaaaaaa agatattgga
     1861 ggaaaatatg gatctgcgcg gaagaagttt tttaaccctg ctggattatt cgccggaaga
     1921 gatccggtac ctgctcgacc tgagccatca ggtcaagcgc gaaaagcgcg agcgcgtgtt
     1981 ccccatgcgg ctgaaaaacc gcaacatcgt tctgctgttc gacaagacca gcacccgcac
     2041 ccgctgcgcg tttgaagtgg ccgcgctgga cgaaggcgcg cacgtaacgt ttttaagcaa
     2101 cagccagatg ggcaagaaag agtcgctcga ggacaccgcc aaggtgcttg gccggtatta
     2161 cgacggcatt gaataccgcg gcttttcgca gaagctggtc gaagaccttg cgcgtttctc
     2221 cggcgtgccc gtttggaacg ggctgacgga cagcgaccat cccacgcagg tgcttgccga
     2281 tcttatgacc atggaagagc atatgaaaaa gccgctcaaa ggcgcgaagc tcgtctacgt
     2341 cggcgacgcg cgcaacaaca tgggcaattc gctcatgatc ggctgcgcca agatgggcgt
     2401 tcactttacc gccattgcac ccaaagcgct ctggccggaa gaggagctga ccgcgaagat
     2461 gcgcgcgctg ggcgagacga ccggctcgaa gatcgagttc tccgaatcgc tcgacgcagc
     2521 caaaggcgcg gacgcggtgt atacggacgt ttgggtgtcc atgggtggga aacgcacttt
     2581 gccgagcgca ttgcattgct ggagccttac cgcgtgacga tggacttaat gaagaaaacc
     2641 ggcaacccgg acgtgctgtt tctgcactgc ctgccgtcgt tccacgattt ggagacggga
     2701 gtgggtaagg aaatcttcga aaagtacggg ctgaaggaga tggaagtcag cgacgaagtc
     2761 ttccgctccc gccacagcgt cgtgtttgac gaggcggaaa accgcatgca tacgattaaa
     2821 gcggtcatgg ttgccacgtt gggggactaa cagacggcaa atccgcctga tttcggggaa
     2881 aaagatgcga aaaagcccga aacagcacgg atattgttga aaacaaaaaa aagttaagat
     2941 ataatagcac aatgcgattt tttgcgccgc agatgcgcgc cgctatcggg ctccaaccgg
     3001 ttcggcgcgg caaaaaaaca aggcgcaacc ggaggaagat taaaaagcta tggaagaacg
     3061 gtatcaaaac aacgttgcgg tgcaggttcc gcgcgttctg ctgccgaaaa ggggaaccaa
     3121 ctatcacaag tgggcggtcg ttgcgtgcga tcagtttaca tcccagccgg agtactggga
     3181 ggaagccaag cgtatcgtcg gcaaggcgcc ttccacgctg gaactgatcc tgccggaagc
     3241 gtatctcgaa aagcccggcg aaaaggagcg gattgccgcc atcaaggcga gcatgcgcgc
     3301 gtaccttgac gaaggcattc tcgaggagaa gccgcaggga ttcgtgctcg ttacccgcac
     3361 cgtagagggc aacacccgca cggggctcgt catggcgctg gatctggaag cgtatgatta
     3421 tcagaaaggc tcgacaacgc cgattcgcgc caccgaaggc acgatcgtat cgcgtattcc
     3481 gccgcggctg aagattcgcg aaggtgcgcc cattgagctg ccgcatatcc tcgtattgat
     3541 cgacgacccg gacagaacgg tcatcgagcc gcttgcgctc aagaaagcga agctgcagaa
     3601 gctctacgat accgacctca tgcaggacgg cgggcacatc accgggcatc tggtggacgc
     3661 ggaggacgat atcctcggcg tgctctccgc gttagagcag ctcaaggacg cggagaagtt
     3721 ccgcgcaaaa tacggcgatc acgcgccgct gctctttgcc atgggcgacg gaaaccacag
     3781 cttcgccacc gcgaaagcct actgggaaaa ggtcaaggag actctgccgg agagcgagcg
     3841 agcggatcat cccgcccggt tcgcgctggt cgaagtggaa aacgtgcatg acgaaggtat
     3901 cgtcttcgag ccgatccacc gcgtggtctt cggtgtaagc ggaaaccgcg ccattgagac
     3961 cctgctgcgt ttcttcaagg agcagaacgg ctgcgcgcga gtagaactgg gacgcatgcc
     4021 ctgcgcgcag agcggggaca cgcatattct gccgtttttg accggagaag tcgagggcgc
     4081 gttcatcgtg agctgcccca agacgcaatt ggctgtcggc accctgcagg cggccatcga
     4141 cgcgctgctg caggaagtgg ccggcaccga ggtggactat atccacggtg cggaagtggt
     4201 caaggaactc tccaaaaagg gcggggcaat cggattcttg ctgccggcca tgaagaagag
     4261 cgagttcttc ccgaccgtgg tctacgacgg cgcgctgccc agaaaaacct tctccatggg
     4321 cgaagcaaac gagaagcgct actacatgga gtgccgccgc atcgagaaga aaaagtaaat
     4381 gggagcgcca aaggggccaa gcccctttgg aaaccccatt cgcttgattt acagggattc
     4441 caaagggacg gcagtccctt tggcgtaatt ccccaaaaac cggtatatct gcggaaaacc
     4501 gcgtaacgat aggaacggga ggaataccaa gtggccgtta aggtagtcaa attcggtgga
     4561 accagtctct gcaacgccga gaatttcaca aaggttaaga acatcatcct tgccgacaag
     4621 gcgagaaagt atgttgtgcc gagcgcaccg ggcaagcgat tccccggcga cgagaagatc
     4681 accgaccttc tctacctctg ctatgcgcag aactcgggcg ggctgagctt tgacgaaacc
     4741 tttacgcaga tcaccgagcg ctatgttgcc attgaaaccg aacttgggct caaaaccaac
     4801 atcgcagccg aactagccgc gatcaagaaa aacatcatct ccggcgcgag cgaggactat
     4861 attgccagcc gcggcgaata cctcagcggc ctgctgcttg cggactttct cggattcggc
     4921 tttctcgacg cggcggagct cgtgagcttc cattccgacg gcacttacga cgacgaaacc
     4981 acccagaagt ggacgcagaa gctcacggat accgagcgcg ttgtcgtccc cggtttctac
     5041 gggcgtaaac gcgacggcag cattaagacc ttttcgcgtg gcg
//
LOCUS       DAOMGY010000019         5022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_16948_length_5022_cov_3.171129, whole genome shotgun sequence.
ACCESSION   DAOMGY010000019 DAOMGY010000000
VERSION     DAOMGY010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16948_length_5022_cov_3.171129"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLR57_00635"
     CDS             complement(<1..462)
                     /locus_tag="PLR57_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39989.1"
                     /translation="MKKNRIAQLLAVVLCAMLLLTTVGCKTRTPAETPEEQPTATEAP
                     VAVLPDVPADRYDATVTPRTGNNATTPLVISSSTLDGKFSPFFVTSAYDTTVESMTQL
                     GLLYLDKKGAPQAGVEFPCLAYSYTQQVSDDKSTSTYKFFLKNGITFSDGAP"
     gene            complement(764..2239)
                     /locus_tag="PLR57_00640"
     CDS             complement(764..2239)
                     /locus_tag="PLR57_00640"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HQQ39990.1"
                     /translation="MNKAKLTRVAIILCAIVACSSFAACSNSGQSADPGAWGYDCKVT
                     YDALGGMVNAREVRNTYYLPNSYVFEPSGSSNMLVQPARDGYIIAGWYRAKTDTPTDL
                     GEEYTFNASDRWDFYLDRVQEDMTLYARWLPQGRVDYVDAGSGEVIFSKNITADSPVQ
                     ELSEAVVEFRKPDGTTLFGYYSDPECTQEYDFASYVHSEPQPTQQQIYDALYEMYPQY
                     LEKVEYVEPDEDEVDATTDTSYLFLNKIGYQLTTTDENALAELRAAKDQLVESYIENY
                     LVNTAGKVVYMKFVEGNFIRVNQPGDMKIGGKYGFFGTDAEGNKIDGYVIESDIDFSG
                     VTLTMSDSFSGVIYGNYHTLSNLKLSLASKKVDKDTEKSLALFNELSDTTIDNLTFEN
                     AAISIQVDQGVTVKAAFLALNAKNVTLTNVKISGMTIDSGKGDTAGTRYTLGDLFVTA
                     SGTKLNDCDGEGLVANVSETAILRLTLLKLEAPEPTPTPAP"
     gene            2682..3935
                     /locus_tag="PLR57_00645"
     CDS             2682..3935
                     /locus_tag="PLR57_00645"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ39991.1"
                     /translation="METVKNKLWTRDFTIITLGTVVSMFGNAVAGFAIGLLVLDYTES
                     ILLYSLFMVCYSLPRIVLPMFAGPYLDRFPRKRVIYTLDFLSCGLYLIIYFLLFKGFF
                     NYLAYLLMSMLIGSIDSVYSVAYDSFYPTLISEGNYTRAYSISSLIYPLSSAIMVPIA
                     GICYKTIGLAPLFLFNAATFLVAAVAETQIRAVETHAEQRASEKFDSRRFVGDLRDGI
                     QYLKRERGLNTIVWFFFVMMLSSSVCMTLALPYFKGLSVGSGIDGVMQYTLVMGANTI
                     GRLIGGGVHYFFRYPKDKKYIIALFVYIAISIIDGAYLFFPFWAMLLFMLADGLLGVT
                     SYNIRISSTQNYLPDEMRGRFNGIFQMFAILGTIIGQLIAGALGDKLPIRGIVAVSMA
                     VTLLSVFLIMVPNGKHVKEIYNVDA"
     gene            4084..>5022
                     /locus_tag="PLR57_00650"
     CDS             4084..>5022
                     /locus_tag="PLR57_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HQQ39992.1"
                     /translation="MFDPRILKLADGLVNFSCSVKPGENVLIESIGGNEDLTRALIKA
                     VYAAGGNPFLWLGDKSYDREIIMHCNVEQLQRRAKVDASLMAQMQAYIGVRGGPNASE
                     MADVPSAKMNEYMKYYWTPVHGEIRVPKTKWVILRYPSPGMAQLADMSTEAFEDYYFN
                     VCTLDYAKMDKAMDSLKALMERTDKVRLVAPGTDLRFSIKGLPAIKCAGRMNIPDGEI
                     YTAPVKTSVNGTIAYNTPSLENGFTYTDIKFTFKDGKIIEATANDTVRINEQLDIDEG
                     ARYVGEFAIGVNPFITFPMKDTLFDEKIAGSIHFTPG"
BASE COUNT         1098 a         1368 c         1293 g         1263 t
ORIGIN      
        1 aggagctcca tcgctgaagg tgataccatt cttcaggaag aacttgtaag tcgaggtgga
       61 cttatcatcg ctgacctgct gggtgtagga atacgcaagg caggggaact caacaccagc
      121 ctgcggcgcg cctttcttat ccagatagag cagcccgagc tgtgtcatgc tttccaccgt
      181 cgtgtcgtaa gcggacgtca caaagaaggg gctgaacttg ccatccagcg tgctggagct
      241 gatgaccagc ggcgtggtag cgttgttgcc ggttctcgga gtgacggttg cgtcatagcg
      301 gtctgccggt acgtcgggca gaacagcaac cggagcttcg gtagcggtag gttgttcttc
      361 cggcgtctct gccggcgttc tggttttgca gccaacggtc gtcagcagca gcattgcgca
      421 aagcacaact gccaggagtt gggcaattct gttctttttc atttcgatac ctccattttt
      481 attttttgag aatcgccgga tggtaccctc caccggcaat tgtcaaatca gttcgcgttt
      541 cagcgcgggt tatacccgtg ccgaaaccga tatcgtttga gaccaccgga tggtacccgc
      601 caccggcaat cgtcaaatcc gtatgagagc ccgctcgcgg gtttacactt gcgagaaccg
      661 atatcgcacg agctttgccg gatggtaccc tccaccggcg gcgctcaaaa ttgaatacgg
      721 atatcttccg gcgcagtaac gtcggaagat atccagtttt tcattacggg gcgggagtcg
      781 gcgtcggctc gggcgcctca agcttgagca gtgtcaagcg aagaatcgct gtctcggaga
      841 cgtttgcaac cagaccttct ccatcgcagt cgttcagttt tgtgccgctg gcggtaacaa
      901 acaggtcgcc aagcgtatag cgtgtccctg ccgtatcgcc tttgccggaa tcgatcgtca
      961 ttcctgaaat cttgacgttc gtcagagtta cgttctttgc attgagggcc agaaacgcgg
     1021 ccttcaccgt aacgccctga tccacttgaa tggaaattgc cgcgttttcg aacgtcaggt
     1081 tatcgattgt cgtatcgctg agttcgttga acagcgcaag tgatttttcc gtgtccttat
     1141 caaccttttt gctggcgagg ctcagtttga ggttggaaag cgtgtggtaa ttgccgtaga
     1201 tcacgccgga gaaactgtcc gacatggtca gggtaacgcc ggaaaagtcg atgtcgcttt
     1261 cgatgacata gccgtcgatc ttgttgcctt cggcatccgt accgaagaag ccgtacttgc
     1321 cgccaatctt catatcgccc ggctgattga cgcgaatgaa gttaccttcc acaaatttca
     1381 tgtagacgac tttgccggcg gtgttgacaa gataattctc aatatacgac tcgacaagct
     1441 gatccttcgc tgcgcgaagt tcggcaagcg cgttttcatc cgtagtcgtc agttggtagc
     1501 cgattttgtt gaggaacaga taggaagtgt ccgtggtggc gtccacctcg tcctcgtccg
     1561 gctcgacata ctctactttt tcgagatact gcggatacat ttcatagagt gcgtcataaa
     1621 tctgctgctg cgtcggctgc ggttccgaat gaacatacga agcaaaatcg tactcctgcg
     1681 tgcattccgg atcggaatag taaccaaaca acgtcgtgcc atccggcttg cggaactcaa
     1741 ctaccgcttc ggaaagctcc tgaacaggag aatcggcggt gatgtttttg ctgaagatga
     1801 cttcgccgct gcctgcgtcg acatagtcca ccctgccctg cggcagccag cgggcataaa
     1861 gcgtcatgtc ttcctgaacg cggtcaagat aaaagtccca gcggtcgctt gcgttgaacg
     1921 tgtattcctc gcccagatct gtgggcgtgt cggtcttggc tctataccag cccgcaatga
     1981 tgtatccatc ccttgccggc tgaacgagca tattgctcga gccggagggt tcgaatacgt
     2041 acgagttcgg caggtaatac gtattgcgca cttcacgcgc gttgaccatc ccgcccaaag
     2101 catcgtaggt taccttacag tcataacccc aggctcccgg gtcggcggat tgccctgaat
     2161 ttgagcaggc ggcgaaagac gaacacgcca ctatcgcgca caaaatgatt gcaacccgag
     2221 tcaacttcgc tttattcatt acgccatccc tttttcagct gtccggaagt gattcttctt
     2281 cctgcatgaa acgcaaaagc acttccgcgc ctagtttaca tattctatct catttattgt
     2341 aattcataca acaaaaagtg tcaataataa gacaatcatt atcaaatacg tcatatttcg
     2401 cctgccgcag agttacaatg tatctatcat atggtctttt ttacgggcgc gtcaacaagt
     2461 ttttccacgc aatctcattt tattgtcagg cgtgtgtatt gaaatacccg aatagcccgg
     2521 attttttgta cgcagagctt tttgtcatat tgtcgctctg tgtgaaccgt ttacacgttt
     2581 tataacgcca aatgatcgtt tataacgttt ttctttcgat agcggtcaaa ctgcaatttt
     2641 cggctttaca accaccagcc gtgcatggta tattacacct catggaaacg gtcaagaata
     2701 aactctggac aagagatttt actattatta cgctcggcac cgttgtctcc atgttcggca
     2761 acgcggtagc gggctttgcc atcgggcttt tggtactgga ttacacggaa tccattctgc
     2821 tgtattccct ctttatggtg tgctacagcc tgccgcgcat cgttctgccg atgttcgcgg
     2881 ggccgtatct ggatcgtttc ccgcgaaaac gcgtcatcta cacgctggac tttctctcct
     2941 gcgggctgta tctgatcatc tacttcctgc tgttcaaagg ttttttcaac tatctggcgt
     3001 atttgctgat gtccatgctg atcggttcca tcgacagcgt atacagcgta gcatacgaca
     3061 gtttttaccc gacccttatc agcgagggca actatacgcg cgcctattcg atttccagcc
     3121 tgatctaccc gctcagcagc gccatcatgg tgccgattgc gggcatttgc tacaagacga
     3181 tcggccttgc gccgctgttc ctctttaacg ccgcaacctt ccttgttgcg gccgttgctg
     3241 aaacgcagat ccgagcggtt gaaacgcatg cggagcagcg cgcgagcgaa aaattcgact
     3301 ccaggcgctt tgtcggcgat ttgcgggacg gcattcaata cctcaaacgc gagcgcgggc
     3361 tcaacaccat cgtctggttt ttctttgtga tgatgctttc cagttcggtc tgtatgaccc
     3421 tcgccctgcc ctacttcaag ggtttatccg tcgggagcgg catagacggc gtgatgcaat
     3481 atacgctcgt gatgggcgcc aacacgatcg ggcgtctgat cggcggaggc gtgcactatt
     3541 tcttccgcta tccgaaggat aaaaagtaca tcatcgcgct gtttgtgtat atcgccatct
     3601 ccattatcga cggcgcttac ctcttcttcc cgttctgggc gatgctgctg ttcatgctgg
     3661 cggacgggtt attgggcgtt acgagctaca acatccgcat ctccagcacg cagaactacc
     3721 tgccggatga gatgcgcggt cgctttaacg gcatattcca gatgttcgcc atcctcggca
     3781 caattatcgg ccagttgatt gcgggcgcgc tgggcgacaa gctgccaatc cgcggcatcg
     3841 ttgcggtctc catggctgtg acgctgctat cggtcttcct gattatggtg cccaacggca
     3901 agcacgtcaa agagatttac aacgtcgacg catagcggca tattccctat aatatattgt
     3961 gaacattgct ttttaacgcc ggagccgctc tggcgttttt gtttatcgtg tgtaataata
     4021 agacgtacgt ctaatcaatc gtttcgaaca acacaaagaa tctgatcgct tggaggaatg
     4081 catatgtttg acccgcgtat cctgaagctg gccgacgggc tggtgaattt ttcctgctcg
     4141 gtaaagccgg gcgaaaacgt actcatcgag agcattggcg gaaacgaaga tctgacccgc
     4201 gccctgatca aagcggtata cgccgcaggc ggcaatccgt ttttgtggct tggcgacaag
     4261 tcctacgacc gtgaaatcat catgcactgc aacgttgaac agctgcagcg ccgcgccaag
     4321 gtggacgctt cgcttatggc gcagatgcag gcttacatcg gtgtgcgcgg aggcccgaac
     4381 gccagcgaaa tggccgacgt gcccagcgcc aagatgaacg aatatatgaa gtattactgg
     4441 acgcccgtcc acggcgaaat ccgcgtgccc aagacgaagt gggtcatcct ccgttacccc
     4501 tcccccggca tggcgcagct ggccgatatg agcaccgagg cgtttgagga ttactatttt
     4561 aacgtctgca ccctcgacta cgccaagatg gacaaggcga tggattcact caaggcgctg
     4621 atggagcgca cggacaaagt tcgccttgtc gcgcccggca ccgacctgcg cttctccatc
     4681 aagggtttgc ccgccatcaa gtgcgcgggg cgcatgaata ttccggacgg cgaaatctac
     4741 accgcgcccg tcaagaccag cgtcaacggc accattgcct acaacacgcc gtcgcttgaa
     4801 aacgggttca cctacacgga catcaagttc accttcaagg acggcaagat cattgaagcc
     4861 accgccaacg ataccgtccg catcaacgag cagctcgata tcgacgaagg cgcgcgctat
     4921 gtaggcgagt ttgctatcgg cgtcaatccc tttatcacct tcccgatgaa ggacacgctg
     4981 tttgatgaga agatagcggg cagcatccac tttacgcccg gc
//
LOCUS       DAOMGY010000020         4914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_17460_length_4914_cov_3.289360, whole genome shotgun sequence.
ACCESSION   DAOMGY010000020 DAOMGY010000000
VERSION     DAOMGY010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17460_length_4914_cov_3.289360"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1761)
                     /gene="atpA"
                     /locus_tag="PLR57_00655"
     CDS             complement(<1..1761)
                     /gene="atpA"
                     /locus_tag="PLR57_00655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HQQ39993.1"
                     /translation="MALKGELYIAGPFGKEQIDSIKAHFTELLGEEVEFDVRRNENLV
                     GGFLAKINGKVYDASVAFRMKEAQHSLVSNSEGIPGAEFVSEDVKLMGAPADRTAREI
                     GSVLKKRLSAYANKNAVYEYGTVTSVGDGVVRIEGLSHTRYGEQISFDRDVFGMTMDL
                     ELDGVGAVLLNGQDEVHVGERVLGTGKVVEVPVGPELLGRVINPIGMPLDGKPLNPSA
                     YRPVESPAPRIIDRQAVNEPLMTGLLAVDSIIPIGRGQRELIIGDRQTGKTTIAVDTI
                     INQRGNGVLCVYVAIGQKLSTIAQIYETLTSTGAMDYSVIVAANASDCAAMQYLAPYA
                     GCSIAEHFMYGGKDVLIVYDDLSKHAVAYRAMSLLLHRPPGREAYPGDVFYLHSRLLE
                     RSGHLNDQLGGGSLTALPIVETMASDISAYIPTNVISITDGQIYLESELFHSGIRPAV
                     NVGLSVSRVGSAAQKKAMRKVAKTLRLTLAQFRELQVFAQFGSDIDSVTKGILERGDR
                     LSETLKQNQHEPLAMSEQVLLLYTVMNGYCKDIPSDAVRAFQKGLLQYAHRNNFELLN
                     ALEETGDLTDDMIKDMINCIE"
     gene            complement(1904..2380)
                     /gene="atpF"
                     /locus_tag="PLR57_00660"
     CDS             complement(1904..2380)
                     /gene="atpF"
                     /locus_tag="PLR57_00660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HQQ39994.1"
                     /translation="MEFHLIDFIEHVLNILIFFLLLRTFLYKPVKKFMDAREAKFSSE
                     RAEIDAERKDADALKTRYEASMADAKIAAERIAEDKRKAVERESEDVREKARREAQRI
                     LTDAKAQAAAEHEEMLGELKSQTAELAVYLAGKILEREVNPKDHQHMIDSFFKRVV"
     gene            complement(2403..2636)
                     /gene="atpE"
                     /locus_tag="PLR57_00665"
     CDS             complement(2403..2636)
                     /gene="atpE"
                     /locus_tag="PLR57_00665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HQQ39995.1"
                     /translation="MTDIGLIAIGAGLAVLTGAGAGLGMGIATGKAVEAVARQPEANS
                     KINALLILGLALTESTAIYGFLTALIMIFTMGK"
     gene            complement(2728..3627)
                     /locus_tag="PLR57_00670"
     CDS             complement(2728..3627)
                     /locus_tag="PLR57_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit a"
                     /protein_id="HQQ39996.1"
                     /translation="MQMPSVGKFIALALSLLLGSAALVFRSRVKLPAADDASFKSVKK
                     KRSLLMMLGSVLIWVFSGLLFGLFTAESKTFEVSINPARMQILGFSVSSSVVIAWIVI
                     AVFLVIALLVRFLLVPRFKETPRGLQLILETAVGAVSDFTKERFGHGNENLSAYFFAL
                     AALFIGSAVVELFGFRPPTADLTMRFSYALVTFILINYYGVRKKGVKGRIKTILKPNP
                     VVSPFKILSDIAVPVSLACRLFGNMFGGMIVIDLLYYALGAFAVGIPAVLGLYFNAFH
                     PLIQFFIFINLSLTFIGEAVEED"
     gene            complement(3635..3904)
                     /locus_tag="PLR57_00675"
     CDS             complement(3635..3904)
                     /locus_tag="PLR57_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ39997.1"
                     /translation="MAGLLIGLVCGAGELFLLTRLTHALQKGESLKILGIVLCKLALF
                     AAAIVPVALYLRSDLLWCGIGISSALIVGAFIVNIVSQRKGKGDR"
     gene            complement(4176..>4914)
                     /locus_tag="PLR57_00680"
     CDS             complement(4176..>4914)
                     /locus_tag="PLR57_00680"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ39998.1"
                     /translation="EHAGENAPCLVYCHGGGFVFGLNPMHIKLVCEYAQNTSCKVLLA
                     DYRLAPKHPFPAGAEDCYAVLQWAAEHANEIGIDPDNIALGGDSAGGALSMSVSLMAR
                     DRGGKAARFLLLIYPVADARQETASMKAFPDTPCWNAKANKRMWELYLANGDAGLRVY
                     ASPLEAESLAGLPDAYLETAEFDCLRDEGAALASALEAAGSRVEYHLIKGAIHGFEIA
                     YKSKLTRESMEQRIHALKRAFGAQSAR"
BASE COUNT         1045 a         1469 c         1360 g         1040 t
ORIGIN      
        1 ctcaatgcag ttgatcatgt ccttgatcat gtcgtcggtc aagtccccag tttcttcaag
       61 cgcgttgagc agctcaaagt tattgcggtg cgcgtactgc aataagccct tctggaaggc
      121 gcgcacggca tcggagggga tatccttgca gtagccgttc atcaccgtgt acaggagcag
      181 cacctgctcg ctcatggcga gcggctcatg ctggttctgc ttgagcgttt cggagaggcg
      241 gtcgccgcgc tccagaatgc ccttggtcac gctgtcgata tccgaaccga actgggcgaa
      301 cacctgcagt tcgcggaact gcgccagcgt caggcgcagg gtctttgcaa ccttgcgcat
      361 agcctttttc tgcgcggcgc tgccgacgcg cgaaaccgaa aggccaacgt tgaccgccgg
      421 gcggatgccg gagtgaaaca gctcggattc cagatagatc tggccgtccg taatggaaat
      481 gacgttcgtc gggatatacg ccgagatatc gctggccatg gtctcaacga tgggaagcgc
      541 ggtcagcgac ccgccgccga gctggtcgtt gaggtggccg gagcgctcca gcaaacggct
      601 gtgcagataa aatacgtctc cggggtacgc ctcgcgtccc ggcggacggt gcagcagcag
      661 cgacatggcg cgataggcca cggcgtgctt ggaaaggtcg tcgtaaacaa tcagcacgtc
      721 cttgccgcca tacatgaagt gctcggcaat cgagcagccc gcgtagggag caaggtactg
      781 cattgccgca cagtcgcttg cgtttgcggc aacgatgacc gaatagtcca tcgctccggt
      841 agatgtaagc gtttcgtaaa tctgcgcgat ggtagaaagc ttttggccaa tggcaacgta
      901 aacgcagagc accccgttgc cgcgctggtt gataatcgta tccaccgcga tggttgtttt
      961 gcctgtctgc ctgtcgccaa tgatgagttc gcgctggccg cggccgatgg ggataatgct
     1021 gtccaccgcg agaaggccgg tcatgagcgg ctcgttgacc gcctgacggt cgataatgcg
     1081 cggcgcgggc gattccaccg ggcggtaagc ggaaggattg agcggtttgc cgtccagcgg
     1141 cataccgatg gggttgatga cgcgcccgag cagctcgggg ccgactggca cttccacaac
     1201 cttgcccgtg ccgaggacgc gttcgccaac atggacttcg tcctggccgt tgagcagcac
     1261 cgcgccgacg ccgtcgagct ccagatccat cgtcatgccg aacacgtccc ggtcaaagga
     1321 aatctgttcg ccatagcgcg tatgcgaaag gccttcgatt cgcacgaccc cgtcgcctac
     1381 ggacgtcacc gttccgtatt cataaacggc gtttttattt gcgtaagcgc tgagcctctt
     1441 ttttaaaacc gaaccgatct ctcttgccgt tctgtccgcg ggagcgccca ttagcttgac
     1501 gtcctcggaa acgaattccg cgcccggaat gccttcactg ttcgaaacca gcgaatgctg
     1561 ggcttccttc atgcggaacg cgacgcttgc gtcatacacc tttccgttga tcttggcgag
     1621 gaaaccgccc accagattct cgttgcggcg tacgtcaaac tcaacctctt cgccgagcag
     1681 ttcggtgaag tgggccttga tggaatcgat ttgctccttg ccgaacgggc cggcaatgta
     1741 tagttcaccc ttcagagcca tatcgtgtct actttctgat gattcactgt ttgcggcaaa
     1801 cgctgccgcg aagtatcctt ggccgccatt tcgcggtagc gaaaccgaac cgttctgcgg
     1861 ttacgcaggc tgatgcgcct gcctgcaagc ttttcctgcg cgtttaaaca acccgcttga
     1921 agaagctgtc gatcatgtgc tggtgatcct tggggttgac ctcgcgttcg agaatttttc
     1981 ccgccagata caccgcgagc tccgccgttt ggcttttgag ttcgccaagc atttcttcat
     2041 gctccgccgc cgcctgtgct ttggcatccg tcaggatgcg ctgcgcttcg cgcctggcct
     2101 tttcgcgcac gtcttccgac tcgcgctcaa cggccttgcg tttatcttcg gcaatccgct
     2161 ccgcggcaat ctttgcgtcc gccatcgagg cctcgtagcg cgttttcagc gcatccgcgt
     2221 ccttgcgttc ggcgtcgatt tccgctcgct cgctgctaaa cttcgcttcc cgcgcgtcca
     2281 taaacttttt tacgggttta tagaggaacg tgcgtaacag caaaaagaat atcaggatgt
     2341 tgagcacatg ctcgataaaa tcgatcaggt gaaattccat tgaaacccct cgaatcttgt
     2401 cgttacttac ccattgtaaa aatcatgatc agcgcggtta agaagccgta gatcgccgtc
     2461 gattccgtca gcgcaaggcc gagaatcaag agcgcgttga tcttggagtt tgcttccggc
     2521 tggcgggcaa ccgcctcaac agccttgccg gtcgcgatgc ccatgccaag gcccgcgccc
     2581 gcgccagtga gcactgccaa acccgcgccg attgcgatta atccgatatc cgtcatggta
     2641 tattcctcct gctctatgct gtaataaagt tggtcctgaa gttgtgctgc ccgcgcgccg
     2701 aatgggtatg cacgcaggcg gggttgtcta gtcctcttcc accgcttcgc cgataaacgt
     2761 cagcgaaagg ttgatgaaga tgaaaaattg tatcagcggg tggaacgcgt tgaagtacaa
     2821 ccccagcacc gcggggatgc caaccgcgaa cgcgcccagc gcataataca acaggtcgat
     2881 cacgatcatg ccgccgaaca tattgccgaa caaccggcag gccagcgaga ccggcaccgc
     2941 gatatccgaa aggatcttga acggtgaaac gaccgggttc ggcttgagga tggtcttaat
     3001 gcgccccttg acgccttttt tgcgcacgcc gtagtagttg atcaatataa acgttaccag
     3061 cgcgtaggaa aacctcatgg tcagatccgc cgtcggagga cggaacccga agagttccac
     3121 cacggcggag ccaatgaaca gcgccgccag cgcaaagaaa taagccgaga gattctcgtt
     3181 gccgtggccg aatctctcct ttgtaaaatc ggagaccgcg ccgaccgccg tttccaaaat
     3241 aagctggagc ccgcgcggcg tttctttgaa gcgcggaacc agcaagaacc ggacgagcag
     3301 ggcaatgacc agaaataccg caatgacgat ccaggcgatc accacagaag agctcacgga
     3361 aaagcccagt atctgcatgc gcgccgggtt gatggagact tcaaaggtct tcgattcagc
     3421 ggtaaacaaa ccaaacagaa gcccgctgaa aacccagatc agaacggatc cgagcatcat
     3481 cagcagactg cgttttttct ttacggactt aaaggaagca tcgtccgcag cgggcagctt
     3541 aacgcgggag cgaaacacca gcgcggccga acccaagagc agcgacagag caagggctat
     3601 gaactttccg acagaaggca tttgcatttc ggacttagcg gtctcctttc ccttttctct
     3661 gagaaacgat attcacgata aacgcgccaa cgatgagcgc ggaggaaatc ccaatgccgc
     3721 accaaagcag gtcgctgcga agataaagcg ccaccggcac gattgcggcg gcaaacagcg
     3781 cgagtttgca gagcacgatt cccagtattt tcaggctctc gcccttttgc aaggcgtgtg
     3841 tcagccgcgt gagaagaaaa agttctcccg ctccgcagac aagaccgatc agcaaaccgg
     3901 ccaatgcaat tcgccccctt cgcggcggtg cgtcgggtag taaatgtgct ccgcgcgccg
     3961 cgtgcgccta cctgccaaaa tcgtatctgg attcgttaaa agcaccgaat ctgcaaatgc
     4021 gacagcaatt ggcaaaattt acgcattttg aatacggtta tcatagcgca gacgttccgg
     4081 gaagtaaagc ccttttttat cgtttctgtt tgatatccgt cattcacggc gccctgtgcg
     4141 gaacaacagg caggtgcgca tagctgttcc gccggtcacc gcgcagattg cgcaccaaac
     4201 gcccgtttca gcgcgtgaat ccgctgttcc atcgattccc gcgtcagttt gctcttgtaa
     4261 gctatttcaa aaccgtgtat cgcgcctttg atcaggtggt attccacgcg ggaaccagcc
     4321 gcttccagcg cgctcgcaag cgccgctccc tcatcccgca ggcagtcgaa ctcagccgtt
     4381 tctaaataag cgtccggcag tcctgcaagc gactccgctt ccagcgggga agcgtataca
     4441 cgcagtccgg catcgccgtt tgccagatag agttcccaca tgcgcttgtt cgccttggcg
     4501 ttccagcatg gcgtgtccgg gaatgccttc atggaagccg tctcctgccg cgcatccgca
     4561 acggggtaaa tcagcagcag aaaacgcgcg gcctttccgc ccctgtcccg tgccatcagg
     4621 ctgaccgaca tggagagcgc gccgcccgcg ctgtccccgc cgagcgcaat gttatcgggg
     4681 tcgattccga tctcgtttgc gtgttccgcc gcccactgca gaaccgcata gcagtcctcc
     4741 gcgcccgccg ggaacggatg cttcggcgcg agccggtagt ccgccagcag caccttgcag
     4801 gacgtgtttt gcgcgtactc gcaaacgagc ttgatgtgca tcgggttcaa accaaacaca
     4861 aaaccgccgc cgtgacaata taccagacaa ggcgcgttct cgcctgcgtg ctcc
//
LOCUS       DAOMGY010000021         4900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_17537_length_4900_cov_3.379154, whole genome shotgun sequence.
ACCESSION   DAOMGY010000021 DAOMGY010000000
VERSION     DAOMGY010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17537_length_4900_cov_3.379154"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..837
                     /locus_tag="PLR57_00685"
     CDS             1..837
                     /locus_tag="PLR57_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQQ39999.1"
                     /translation="MLPRHKRLVKRNWILFIILMIGALSMLFPFVWMTLSSFKTKADV
                     YSFPPRWIPTVWTWDNFKRVFEMIPFLRYYLNSIYTSLLQTGLVIGISILGAYALTKL
                     RFPGQKVYSMFLLSSMFVPAVVTMIPLYLVVSSMGLNDTYAGIVLPQISTAFTTMLLS
                     SFFSAIPNDLVDAARLDGCGHFGVLTKVVMPNSKGAISTATLFTFLGAWKSYTWPLII
                     TTKTEFRTLPIGLKYLVTESSSEYQVMMAASLMAILPVLLVFIFAEKQLVRSITLTGM
                     KS"
     gene            899..2302
                     /locus_tag="PLR57_00690"
     CDS             899..2302
                     /locus_tag="PLR57_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ40000.1"
                     /translation="MKRTTKLTALLLAVLLLLFTVVGCLPAPAAVNGSDSREDAAAAE
                     TAEGETSGTTEAALPAVDLTKAMDPAERTPQTLEVWYAVSGTSGEKFTEQATAFDEAN
                     DLVSLNLSYSGGSNDTATKVSAALLTGTEPDVALMYAGPSFTGSLGNFDMAQLVQREG
                     FNKDDIYPGIWDYCKYFNGEICAVPYGISTPVMYYNKDILAAAGVDMTNPPKTWAEFY
                     DVCLQCLEKGNINGMDGFTAFDVSETGWLFKSMCMQNGCPVVEVNDGVITPVFNSPEA
                     LEVAEYWKSLVDAGIMGASEHSAAENKFLAGNLAFVVMSSNRMSRWTDVDINIGAIEM
                     PYFKQQSVALGGNVLVIFTQDPQKIEAAWDFITYLLQPEQNLDFALSTGYLPVRQSEL
                     DSDAVTAAIAQNEMYAVAFKQLSYAWAYVHFEQMGTMDLQIADALSRIEKGTKEPKQA
                     LDDALNKLVTEMEFDAQ"
     gene            2375..3163
                     /locus_tag="PLR57_00695"
     CDS             2375..3163
                     /locus_tag="PLR57_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HQQ40001.1"
                     /translation="MKNSERYSFSTGALYPLESEDALKQIKNAGFPNAELMPQSLSDS
                     SKESAKRLERIGLRVASIHYPLAFFPLLYTAHRSMRDDGRKYSLELLALGERLGTEVL
                     VVHPHIPSNKGYDALLDDPVVENLLWLAEECQKRGILTAMENSPKTCGTPELLLTYIA
                     KLNHPNIKPMVDTTEAREAGGDPAPFIAAVSPCHLHISDYMGESKHLPAGEGDTDFAA
                     VARELKDYQGFYTLEPAYRYYIENTEQKLRAGYAFLARTFPDTL"
     gene            3213..4190
                     /locus_tag="PLR57_00700"
     CDS             3213..4190
                     /locus_tag="PLR57_00700"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HQQ40002.1"
                     /translation="MVNTDKIQSIFTALAVGDAVGMPTEFMTRLEIQNRFHAFVPGLV
                     DTKFSRHHADLPFASVTDDTEQNLYLYRLYLVSKRVDAKETASCLLRWAEETGAEQKR
                     YIGPSSLSALNAIRAGAPVEEAGRYGTTCGGIMRTPAAVLYRKHTTDDDLARDILACL
                     LCTHNTSEALESAGAYGFALRAALDGGSRKEILAEAIRGANRLIALAPEINCAPSSAV
                     RVKAMYSLAATHSPEHLLDTLYNLYGTGLPSADVCGAVFAIFFSAREDVWKAIRMGAS
                     IGGDTDTIASLAGALCCAFAGGHNIPDAIVGSVFSSNPILRGAPWEAAI"
     gene            4187..>4900
                     /locus_tag="PLR57_00705"
     CDS             4187..>4900
                     /locus_tag="PLR57_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ40003.1"
                     /translation="MSRNQQQFDLLIVGGGAVGSAIAYRLSRYQLKIGVLEKEPDIAI
                     GTSGRNSAVVHAGFNNRTGSLMAKLCVEGNKGFEEAAKTLDVPYRKTGKLVVGYNDVD
                     RDILLRLIETGEQNGCEGLRLIDEDEIRALEPNVPAKWAMLSPNTAIINPFLYNIHLA
                     EAAAQNGVTYLLNREVTAIERVSGGFYVTAGDETFECSLLVNAAGLFADRISAMAGDG
                     RYTIYPCRGEYYLLDKIPDG"
BASE COUNT         1042 a         1488 c         1376 g          994 t
ORIGIN      
        1 atgcttccaa gacacaaaag actcgtcaaa cggaactgga ttctgtttat catcctcatg
       61 atcggcgcgc tgagcatgct ctttccgttc gtctggatga cgctttcttc gttcaaaacc
      121 aaggcggacg tctacagttt cccgccgcgc tggattccca ccgtctggac atgggataac
      181 tttaagcgcg tgttcgagat gattccgttt ctgcgctatt atctcaacag catctatacg
      241 tcccttctgc agacggggct ggtcatcggc atttccatac tgggcgctta cgcgctcacc
      301 aagctgcgct ttccggggca aaaggtatac tccatgttcc tgctcagcag catgtttgtt
      361 ccggcagtgg tcacgatgat tccgctgtat cttgtcgttt cctccatggg gctcaacgat
      421 acctacgcgg gcatcgtcct gccgcagatc tccacggcgt ttacgacgat gctgctttcg
      481 agtttctttt ccgccattcc aaacgacctt gtggacgcgg cgcggctgga cggctgcggg
      541 cacttcggag tgctcaccaa ggtggtcatg cctaacagca agggcgcaat ctccacggct
      601 acgcttttta ccttcctcgg cgcatggaag agctacacct ggccgctgat cattacgacc
      661 aagacggagt tccgcacgtt gccgatcggc ttaaagtacc tcgtcaccga gtcgagcagc
      721 gagtatcagg tgatgatggc ggcgtcgctc atggcgattc tgccggtgct ccttgtgttc
      781 atctttgcag aaaaacagct ggtgcgctcc attacgctca ccggcatgaa gtcctgacgc
      841 ggcgtgaatc atcatgcggc gcgaacggtt cactaaaaaa tcaaaggggg attcgatgat
      901 gaaacgtaca acgaaactga ctgccctgct tcttgcggtg ctcttgcttc tctttacggt
      961 ggtcggatgc ctgcccgcgc ccgccgccgt gaacggttcg gactcgcgcg aggatgcagc
     1021 cgccgctgaa acggccgaag gcgaaacgag tggaaccacc gaagcagcgc tgcccgcggt
     1081 cgatctgaca aaagccatgg atcccgccga gcgtacaccg cagacgctgg aagtctggta
     1141 cgccgtctcc ggaacgagcg gtgaaaagtt taccgaacag gcgaccgcct tcgacgaggc
     1201 aaacgacctc gtctccctca acctctccta ctccggcggc tccaacgaca ccgcaaccaa
     1261 ggtcagcgcg gcgctgttga ccggcaccga gccggacgtt gcgctcatgt acgccgggcc
     1321 ttcgtttacg ggcagcctcg gcaatttcga catggcgcag ctcgtgcagc gcgaagggtt
     1381 caacaaggac gatatctacc ccggcatctg ggactactgc aagtatttca acggagaaat
     1441 ttgcgcggtt ccctatggca tctccacgcc ggtgatgtat tataataaag atattctcgc
     1501 ggcggccggc gtcgatatga caaatcctcc gaagacttgg gccgagttct acgacgtttg
     1561 cctccagtgc ctcgaaaagg gcaacatcaa cggcatggac ggctttacgg cgttcgacgt
     1621 ttcggaaacc ggctggctgt ttaagtccat gtgcatgcaa aacggctgcc cggtggtgga
     1681 agtcaacgac ggcgtcatca cccccgtgtt caacagtccg gaagcgctgg aagttgcgga
     1741 atactggaag agcctcgtgg atgccggtat catgggcgca agcgagcact ccgccgcgga
     1801 aaataagttc ctcgcgggca acctggcgtt cgtcgtcatg tccagcaacc gtatgtcgcg
     1861 ctggacggac gtcgacatca acatcggcgc catcgaaatg ccgtacttca agcagcagtc
     1921 ggtcgcgctc ggcggcaacg tgcttgtcat ctttacgcag gacccgcaga agatcgaagc
     1981 cgcgtgggac ttcatcacct acctgctcca gccggagcag aacctcgatt tcgcgctttc
     2041 cacgggctat ctgccggtcc gccagtccga gctggattcg gacgcggtca ccgcggcgat
     2101 tgcgcagaac gagatgtacg ccgttgcgtt caaacagctc tcctatgcct gggcgtatgt
     2161 ccactttgaa cagatgggca ccatggacct gcagattgcg gacgcgctga gccggatcga
     2221 aaagggcacc aaggaaccga agcaggcgct ggacgacgcg ctcaacaaac tcgtaaccga
     2281 aatggagttt gacgcgcagt aacgaaccaa agaaccgttt ggcgcgacgg ggcgcttcct
     2341 cgctccgccg cgccattttt ataggtgaaa cagtatgaaa aattccgaac gctattcctt
     2401 ttccaccggc gcgctctacc ccctcgaatc ggaggacgcg ctgaagcaaa tcaaaaacgc
     2461 gggcttcccc aacgcggaac tcatgccgca gagcctttcg gactcttcca aagagagcgc
     2521 aaagcggttg gagcggattg gtctccgcgt ggcttccatc cattatccgc ttgcattttt
     2581 cccgctgctg tataccgcgc accgctccat gcgtgacgac ggcagaaaat attcgctgga
     2641 actgctcgcg ctgggcgagc ggctcggcac cgaagtgctg gttgtgcacc cgcacatccc
     2701 ttccaacaag ggctacgacg cgctgctgga cgacccggtc gtggaaaatc tcctttggct
     2761 cgcggaggaa tgccaaaaac gcggcatcct cacggcaatg gagaactcgc ccaagacctg
     2821 cggcacgccc gagctgctgc ttacctatat tgcaaagctg aaccacccga acatcaaacc
     2881 catggtggat acgaccgaag cgcgcgaggc gggcggcgac cccgcgccgt ttatcgcggc
     2941 tgtttcgcca tgccacttgc atatttccga ctacatgggc gaaagcaagc accttcccgc
     3001 gggcgaagga gacacggact ttgccgcagt tgccagagag cttaaagatt atcaggggtt
     3061 ctacacgctg gaacccgcgt accgctatta cattgaaaac accgaacaga agctgcgcgc
     3121 aggttacgcg tttttagcca gaacattccc ggacaccctg taggaacact atcgcaagga
     3181 caaccaattt cttcgaaagg cagacacaac ccttggtcaa tacggacaaa attcaatcca
     3241 tctttaccgc gcttgcggtt ggggacgcgg tcggtatgcc gacggagttt atgacgcgtc
     3301 tggaaattca gaaccgcttc cacgcgtttg tgccggggct ggtcgatacc aaattctcgc
     3361 gccatcacgc ggatcttccg tttgcttccg tcaccgacga tacggagcag aacctctatc
     3421 tctaccggct gtacttggtt tctaaacgcg tggatgcaaa agaaacagct tcctgccttc
     3481 tccgctgggc ggaggaaacc ggcgcggaac aaaagcggta tatcggcccc agctcgctct
     3541 ccgcgctgaa cgccatccgc gcgggtgcgc ccgtggagga agcgggcaga tacggaacca
     3601 cctgcggcgg cattatgcgc acgccggcgg cagtgctgta ccgcaagcac accacggacg
     3661 acgatcttgc gcgggatatt ctcgcttgcc ttctctgcac gcacaacacg agcgaagcgc
     3721 tggaatctgc gggcgcttac ggttttgctc tacgcgccgc gctcgacgga ggcagtcgta
     3781 aagaaattct tgccgaagcg attcgcggag ccaatcgctt aatcgcgctc gctcccgaaa
     3841 ttaactgcgc tccctcttcc gccgtgcgcg ttaaagccat gtattcgctg gcggcgacgc
     3901 attcgccgga acatcttcta gatacgctct acaacctcta cggcacggga cttccctccg
     3961 cggacgtctg cggcgcggtg ttcgccatct tcttttccgc acgggaggac gtctggaaag
     4021 ccatccgcat gggcgcttcc atcggcgggg acacggacac catcgcgtcg cttgcgggcg
     4081 cgctctgctg cgcgttcgcg ggcgggcaca acattccgga cgcgattgtg ggcagcgtgt
     4141 tttccagcaa tcccattctg cgtggtgcgc cctgggaggc agcgatttga gccggaacca
     4201 gcagcaattt gatctcctta tcgttggcgg cggcgcggtc ggcagcgcga tagcctaccg
     4261 tctgagccgc tatcaactga aaatcggcgt actggaaaaa gaaccggaca tcgccatcgg
     4321 caccagcggg cgcaacagcg ccgtcgtgca cgcggggttc aacaaccgca cgggctcgct
     4381 catggccaag ctctgcgtcg agggtaacaa aggctttgag gaagcggcaa agacgctgga
     4441 cgtaccctac cgcaagaccg gaaagctcgt cgtgggctat aacgacgtgg atcgggatat
     4501 cctcctgcgc ctcatcgaaa ccggcgaaca gaacggctgc gaaggtcttc gcctgatcga
     4561 cgaggatgaa atccgcgcgc tggagccgaa cgtgcccgcc aagtgggcaa tgctttcacc
     4621 caacaccgca atcatcaatc catttttata caatatccat ctggccgaag ccgccgcgca
     4681 aaacggggtg acgtatctgc ttaaccgcga agtcaccgct atcgagcgtg tctccggcgg
     4741 tttttatgta accgcaggcg acgaaacgtt cgaatgctcc ctgctcgtca atgcggcagg
     4801 gctgtttgcc gaccgcatca gcgctatggc gggcgacggc cgctatacga tttatccctg
     4861 ccggggggaa tactacctgc tcgataaaat cccggacggt
//
LOCUS       DAOMGY010000022         4866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_17729_length_4866_cov_3.283725, whole genome shotgun sequence.
ACCESSION   DAOMGY010000022 DAOMGY010000000
VERSION     DAOMGY010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17729_length_4866_cov_3.283725"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1316)
                     /locus_tag="PLR57_00710"
     CDS             complement(198..1316)
                     /locus_tag="PLR57_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40004.1"
                     /translation="MVTIKEAVTKKELVDFMEFPITLYRGNPYYVPDILSSQVADMQK
                     DKNPAFAFCDAKCFLACRDGKIVGRIAGILNTKANAKFGKKYMNFTHIDFIDDNEVVD
                     ALFDTLEGWARSLGCEAVHGPLGFSDMDREGMLVEGFDQLSLFYTYYNHPYYKDQLER
                     RGYAKQVDWLEVRLTIPQTPDESLTRVANYVKKRMKLRLVSLNDKPIKILMRDMFKLY
                     NDTYQVLFGMVPMTDEQMLKYTSEFRPMVDARTTSFVYNEEGEMVAFGICCPALDHAM
                     QKMKGRTLPFGWIPLLRALKGKNDTVDLLLIAVRPDLQGLGVNAIILDEMQHKLVKAG
                     FKYAESGPMLEINDNILAQWARFENVQHKRRRCFVKEL"
     gene            complement(1350..2348)
                     /locus_tag="PLR57_00715"
     CDS             complement(1350..2348)
                     /locus_tag="PLR57_00715"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HQQ40005.1"
                     /translation="MKNKRAWIALLVSIVMLLSIGCASKAAEQSIAPAAGASYDTSYA
                     EPAMEAVTSESFSSSDSSQANAPQSETDPAGDFDVRKIVYNANMTITADDPELVLNNL
                     IAKAESLGGYVSGSYTRTDDLGATVSTATLKVPASQLDALVTAAKAAGKVDEYQLNSD
                     DISLSYYDMQARLDNAKAEEQQLLEILAKCETVEEILAVRESLTSVRADIESYTAQMN
                     LWDNLVAYATLYVTINRTPKTAVEGEKDLIQIWKASDVWNKMARGFANSARFVANALG
                     AIGIFLAIAAVPAGVLFLAIGLPIILHKKKKRRQAAEKAKEAEYSAEPDDTTKQNK"
     gene            complement(2532..2690)
                     /locus_tag="PLR57_00720"
     CDS             complement(2532..2690)
                     /locus_tag="PLR57_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40006.1"
                     /translation="MEVGASDRIVSFCVCLQDDSTQRLLVSCKITSPGEATVLPDGTS
                     MTIGDVQP"
     gene            complement(2751..3323)
                     /locus_tag="PLR57_00725"
     CDS             complement(2751..3323)
                     /locus_tag="PLR57_00725"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HQQ40007.1"
                     /translation="MKRKKRTRADKITTVLYVIFGLLVAAALYGLGSLGYTLLGEQVG
                     NSYYANLAEKSRAEDSVDFSSLSAANPEIKAWVRLDGTAIDLPIVQTTDNSFYLTHRF
                     DEKKNKLGTPFIDMTNAGDFSDRHTVIYGHAVKSGALFGSFWEYENPNYFNRNPELSL
                     YLPDGTQYILKVFACSRVDGVRSAIPTSFA"
     gene            complement(3430..4269)
                     /locus_tag="PLR57_00730"
     CDS             complement(3430..4269)
                     /locus_tag="PLR57_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ40008.1"
                     /translation="MIVPNAQTDDFRLEEHENGFCRIIDPMGVGGTLIRGNERALLID
                     TGFGLMDIRPAVRRATSLPLIVMNSHIHTDHSGGNYLFDTVYIPEEEFEKLSDGSLDR
                     ERDSLFQSWRFIRPHLKQYMTEGVRLTERILKTRYLPLPDTFDLGGRSLEIMKLGGHT
                     RASSVVIDPSTRTAFVGDAISPTLWLFLYPTGTIAQYAEKLDAFCQRTDIDWLQLSHK
                     STPIPFAFAAYLRDFVLRARIERSEIWPNNRFSVTVYRYAEQDTPYGEMELFFCETNL
                     QKD"
     gene            complement(4353..>4866)
                     /locus_tag="PLR57_00735"
     CDS             complement(4353..>4866)
                     /locus_tag="PLR57_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HQQ40009.1"
                     /translation="YTPTEGLVMKSRCGDIDPYILVYLMEDCGYTLAQVKTMMNKQSG
                     MLGMSGVSNDLRDVERAAEEGNADAAIAIESYCYQIKKQIGAYAAAMGGVDAIAFTGG
                     IGENSALVRSKSLRGLAYMGVLLDERKNESAVCDCELTAQGGSVRLFAIAANEELVVA
                     KKAKEYLERR"
BASE COUNT          996 a         1418 c         1287 g         1165 t
ORIGIN      
        1 gtcatgaacc agaaatcata ttgacgaacg aatccgccga tatagggaat accgacatac
       61 ttgctgataa tcgccagcgc ggcgagaatc agcgcgagca gccatatagc ccctttgcgt
      121 cttgacagtt gcatgtttct catcctcctg cacttttgtt ttgtatttac ccccgcgtta
      181 ttgcgtggga aaaaccgtta cagttccttg acgaaacacc tgcggcgctt atgctgtacg
      241 ttttcaaacc gcgcccactg cgcgaggata ttgtcgttga tctccagcat cggtccgctc
      301 tcggcgtact taaagccggc tttgacgagc ttgtgctgca tttcgtcgag aatgatggcg
      361 ttgacgccca gcccctgcag gtcgggacgc accgctatga gcagcaaatc gaccgtgtcg
      421 tttttgccct tgagcgcgcg cagcagcgga atccatccga acggcagcgt acgccccttc
      481 atcttctgca tcgcatggtc gagcgccggg cagcaaatgc cgaacgcaac catttcgcct
      541 tcttcgttat acacaaacga agtcgtgcgc gcgtccacca tcggacggaa ctcgctggtg
      601 tatttgagca tctgctcgtc cgtcatcggc accatgccaa agagcacctg ataggtgtcg
      661 ttgtagagct tgaacatatc ccgcatgagg attttgatcg gcttgtcgtt caggctgacc
      721 agacgcagct tcatgcgctt tttaacatag ttggcaacac gcgttagcga ttcgtccggc
      781 gtttgcggaa tggtcaggcg cacttccagc cagtctacct gctttgcgta gccgcggcgt
      841 tccagttgat ccttatagta cgggtgattg taataggtgt agaacaggct cagttgatcg
      901 aagccttcca ccagcatgcc ttcgcggtcc atgtccgaaa agccgagcgg cccgtgcacc
      961 gcttcgcagc cgagagagcg cgcccaacct tcgagcgtat caaagagggc atccaccact
     1021 tcgttgtcgt cgatgaagtc gatatgcgtg aagttcatgt atttcttgcc gaacttcgcg
     1081 ttcgccttcg tattgagaat tcccgcgatc ctgcctacga tctttccgtc gcggcaggca
     1141 agaaagcact ttgcgtcgca aaacgcaaac gccgggttct tgtccttctg catatccgca
     1201 acctggctgg aaagaatgtc cggcacatag tacgggttgc cacggtacag cgtaatcggg
     1261 aactccatga aatcgaccag ttcttttttg gtcactgcct ctttgattgt taccacgaat
     1321 ccatccccct gtatgttaga aatttcagtt tacttgtttt gttttgtcgt atcgtccggt
     1381 tcggcggaat actccgcttc ttttgccttt tcggcggcct gcctgcgttt tttcttttta
     1441 tgcagaataa tcggcagtcc gattgccagg aacagcacgc ccgcgggcac tgcggcaatt
     1501 gcgaggaaaa tgccgattgc gccgagcgcg ttcgctacaa accgcgcgct gttggcaaag
     1561 ccgcgcgcca tcttattcca aacgtcggaa gctttccaaa tctggatcag gtctttttcg
     1621 ccttccaccg cagtctttgg cgtgcggttg atggtgacat agagagtagc gtacgccaca
     1681 agattgtccc agaggttcat ctgcgcggtg tagctttcaa tatccgcccg tacgctcgtc
     1741 aggctttccc gcacggcgag gatctcctcc accgtttcgc acttggcgag aatttccagc
     1801 agctgctgtt cttccgcctt ggcgttgtcg aggcgcgcct gcatatcgta gtacgaaagc
     1861 gagatatcat ccgaattgag ctggtattcg tccactttgc ccgcggcttt cgcggcggtt
     1921 accaaagcgt ccagctgact tgccggaacc ttgagcgtcg cggtggaaac cgtagcgccc
     1981 agatcgtccg ttcgggtgta cgagccggaa acatatccgc cgagtgactc cgcttttgca
     2041 atcaggttgt tcagcacgag ttccgggtcg tccgccgtta tggtcatatt ggcgttgtag
     2101 acgattttcc gtacgtcaaa gtcccccgcc gggtcggttt cactctgtgg ggcatttgcc
     2161 tggctggagt ccgaggaaga aaagctttcg ctggtcaccg cctccatggc gggttccgca
     2221 taggaggtgt cgtaagatgc gcccgccgcg ggcgcgatgc tctgctctgc cgctttcgac
     2281 gcacagccaa tcgacagcag cataacaatg ctgacgagca gcgcaatcca agcgcgtttg
     2341 ttcttcatgt tcgtgccccc ctattcaaga ttttgatacc gcgtatagaa taccacactt
     2401 tttaccaaat cgccagcggg attgcttgtc actattccgg cggcaaaaca acggttttca
     2461 acgcccgcga agcctgtgaa aacagccgcg gaaaacgcgg ctgctatggg agaattcact
     2521 gtttgttatt gctacggctg cacgtcccca atggtcatgc tcgttccgtc cggcagcacg
     2581 gtcgcttcgc cgggagaggt gattttgcag ctgacgagca ggcgctgcgt cgagtcatcc
     2641 tgcaggcaga cgcagaagga gacgatgcgg tcggaagcgc caacttccac attgctcgta
     2701 aacgcggaga gctcgtgcag atcgtcaagg aacgataaaa acgcgttgtc tcaggcaaac
     2761 gaggtcggaa ttgcgctgcg aacgccgtcc acccttgagc aggcaaacac cttgaggata
     2821 tactgcgtgc cgtccggcag gtagagcgac agttcggggt tgcggttgaa gtagttcggg
     2881 ttttcatatt cccagaagga gccgaacagc gcgccgctct tgaccgcgtg gccgtagatt
     2941 accgtatggc ggtcggaaaa atcgcccgcg ttcgtcatgt cgataaaagg cgtgccgagt
     3001 ttgttctttt tttcgtcaaa gcggtgcgtt aagtaaaacg aattgtccgt cgtctgcacg
     3061 atcggcagat cgatcgccgt gccgtccagc ctaacccagg ctttgatctc cgggttcgcc
     3121 gcgctgagcg atgaaaagtc tacggagtct tccgcgcgtg atttttcggc gagattggcg
     3181 taataggaat tgccgacctg ctcgcccagc agcgtgtagc cgagagaccc cagcccgtaa
     3241 agcgccgccg caaccagcag gccaaaaatc acgtagagta ccgttgttat cttatccgct
     3301 ctggttcttt ttttacgctt cataaacgca gtatagccca agaaaactgt ttttgcaatc
     3361 gtggcaaacg tacttcacat tctgttcacg caatcccata cgatcctccc gcagacgttc
     3421 cgccgctttt cagtcttttt gcaaattcgt ctcgcaaaag aagagctcca tctccccgta
     3481 gggtgtgtcc tgctcggcat agcgatacac cgtaacgcta aagcggttat tcggccagat
     3541 ctccgagcgc tcaatacgcg cgcgcagcac aaagtccctt aaatacgccg caaacgcaaa
     3601 cggaatcggc gttgacttat gcgaaagctg cagccagtcg atatccgttc tttggcaaaa
     3661 cgcgtccagc ttttcggcat actgtgcaat cgtgcctgtc gggtacaaaa acagccagag
     3721 cgtcggactg atcgcgtcgc cgacgaacgc cgtgcgcgtg gaaggatcga tcacaacgga
     3781 gctcgcgcgg gtgtgcccgc ccagcttcat aatctccaga ctgcgcccgc caaggtcgaa
     3841 cgtatccggc aggggcagat agcgcgtctt aagaattcgc tccgtcaggc ggacgccttc
     3901 cgtcatatac tgcttgaggt gcgggcgaat gaaccgccag ctttgaaaca gcgaatcccg
     3961 ctcgcggtcg agggatccgt cgctgagctt ttcaaattcc tcttcgggaa tgtagaccgt
     4021 gtcgaacaga taattgccgc cgctgtgatc ggtatgaata tgcgaattca taacgatcag
     4081 cggaaggctt gtcgcgcggc gcaccgcggg acggatatcc atcagcccga atccggtatc
     4141 gatcaacagc gcccgttcgt ttcctcgaat cagcgttcct ccgaccccca tcgggtcgat
     4201 gatgcggcaa aagccgttct cgtgctcttc cagccggaaa tcatccgtct gtgcgtttgg
     4261 tacgatcacc cgtgcagttc ctccagaata acccacgccc gcccggcaaa cccggcaggc
     4321 agatacttgt catattgctt gttttgcctt aatcagcggc gctccaaata ttctttcgcc
     4381 ttcttcgcca ccacgagttc ctcgttggcg gcaatggcaa acagtctgac gcttccgccc
     4441 tgcgcggtca gttcgcagtc acagacggcg gattcgtttt tgcgctcgtc cagcagaaca
     4501 cccatatacg ccagcccccg caggctttta cttctgacca gcgcgctgtt ttcgccgatt
     4561 ccgcccgtaa aggcgatggc gtccacgcca cccattgccg cggcgtacgc gccgatctgc
     4621 tttttaatct gatagcagta gctctcaatc gcaattgccg cgtccgcgtt gccttcctcg
     4681 gctgcacgtt ctacgtcccg caaatcgttg gatacgccgg acatgccgag catgccgctc
     4741 tgcttgttca tcatggtctt gacctgcgcg agcgtgtacc cgcagtcctc catcagatag
     4801 acgaggatgt aagggtcgat atccccgcag cgggatttca tcaccagccc ttcggtcggg
     4861 gtatag
//
LOCUS       DAOMGY010000023         4826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_17936_length_4826_cov_3.850136, whole genome shotgun sequence.
ACCESSION   DAOMGY010000023 DAOMGY010000000
VERSION     DAOMGY010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17936_length_4826_cov_3.850136"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1111
                     /locus_tag="PLR57_00740"
     CDS             242..1111
                     /locus_tag="PLR57_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40010.1"
                     /translation="MKSKKKVLLFAAIGLTTLASAALLGVYFLPSWIPSHSTLEQNPA
                     SAVVEQISDNEVLLAAPASFDAYTAGDTVQLSYLTQPTKRVKPTIQQEEIQPTADDPA
                     PAEPTQEEPAPEEPTPQEPAPEVPTPEEPAPEEPAPEEPAPEDPAPEEPAPEEPTRAV
                     KPSDISDVNAMAEYLYLAGCEFWATEKDFCGTTTRPASTRVADKIGGGTAVDCPGGDT
                     VRIDSNGVSHDSYAIADHFREYVDMPDLQYFKLYLSDKDYTVQQYGARDEVIGAFYQY
                     GSERATYLPEEQE"
     gene            1689..2594
                     /gene="rarD"
                     /locus_tag="PLR57_00745"
     CDS             1689..2594
                     /gene="rarD"
                     /locus_tag="PLR57_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HQQ40011.1"
                     /translation="MKERNVGILCTASCYVLWGLLPLYWHMLDEVDAFVILANRIVWS
                     AVFTIVLLLALRRFGDVKAVLRDRAKMRFVIPAAVMITINWGLYIWAVNAGHLLDASL
                     GYYLNPLMVFAVGMLLFREKCGALDWVALALATVGVLVATLAYGAFPWVAISLALSFA
                     LYGTFKKLGGVSGLTSIAVETILVAPFALGFLLLSPASHAAFSALTLKTGLLLFFTGV
                     VTATPLILFTHGVNRLPFTTVGFLQYISPTLQLIIGTLFFHETLTQDRVVALVFFGVA
                     LVLYSIGITRRAKKQSALETGGLTR"
     gene            2829..>4826
                     /gene="secA"
                     /locus_tag="PLR57_00750"
     CDS             2829..>4826
                     /gene="secA"
                     /locus_tag="PLR57_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQQ40012.1"
                     /translation="MGLIDTLLGNTSASRLKKLQPYIKRINEMEPQMQAMTDEQLRAQ
                     TQKFRERLKNGETLDDIMVEAFATVREAAVRTVDMRHFDVQLLGGMVLHQGRISEMKT
                     GEGKTLVATLPAYLNALTGEGVHVVTVNDYLAKRDAQWMGKIYRFLGMSVGCVINGME
                     RDAKQEAYRSDITYGTNNEFGFDYLRDNMVVFREQMVQRELAYAIVDEVDSILIDEAR
                     TPLIISGEGEESSEMYIEANRFVRGLTRGSNLTQQSSSDRIMGEEIPEDGDYQCDIKK
                     RTVQLTAEGIRKAEERFSLENLGDPANMEINHHIVQALRANALFERDKDYVVQDGEVL
                     IVDEFTGRLMIGRRYSEGLHQALEAKEGVTVEKENRTLATITLQNYFRMYKKLSGMTG
                     TAKTEESEFMSIYDLDVVEIPTNRPLMRTNLDDAIFPTEKGKFDSIVEAIKEIHKTGQ
                     PVLVGTISVERSEYLSGRLTRAGIPHDVLNAKQHAREAEIVAQAGKKGQVTIATNMAG
                     RGTDILLGGNPDFLARRDLRQDGLEDWLIEEAVGHGETADPDILAARARYKELYDAHK
                     VVTSKEHDEVEALGGLFILGTERHESRRIDNQLRGRAGRQGDRGSTQFYVSFEDELMR
                     RFGGDRVTNTLNRFASPEMQQEIRLEMRMLTKQIEAAQKRVE"
BASE COUNT         1150 a         1298 c         1380 g          998 t
ORIGIN      
        1 ttgcgcagcc gccgggaggg taggtctgca gtgagagata aagcgaaccg taaactaata
       61 tcaatagaaa agctaagagt acgcttaaaa gaagtaacat aaaagtaaca aattgcgaca
      121 tattacattc atgtttcatg aatgcgctaa aaatagttcg gataaacacg gtattagaaa
      181 cggatatgta acaatacggg actataatag tgtacagtca aggaaagttg agggatatcc
      241 agtgaaaagc aagaagaaag tactgttgtt tgcggcaatc ggactaacga cgctcgcatc
      301 ggccgcattg cttggcgtat atttccttcc gagctggatt ccttcccatt ccacactcga
      361 acaaaacccg gcgagcgcag ttgtggagca gatttcggat aacgaagtac tattggccgc
      421 gcccgccagc ttcgatgcgt atacagcagg agacaccgtt cagctttcgt acctgacgca
      481 gccgacaaag cgcgttaaac cgacaatcca gcaggaagag attcagccga cggcggatga
      541 ccctgctcct gcagagccaa cacaagaaga acccgctccc gaagaaccta caccgcaaga
      601 acccgcgccg gaagtgccca cacccgaaga acccgcgccg gaagagcctg cgccggaaga
      661 acccgcaccc gaagaccccg ctcccgaaga acccgcgccg gaagaaccga cccgcgccgt
      721 caagccaagc gatatatcag acgttaatgc gatggccgaa tatctctatc tcgccggctg
      781 cgagttctgg gcgactgaaa aggacttttg cggtacgacc acccgccccg cctccacccg
      841 cgttgcggat aaaatcggcg gcggcacagc cgtggactgc ccgggcggcg acacggttcg
      901 catcgacagc aacggagtaa gccacgacag ctatgctatt gcagaccact tccgcgaata
      961 tgtggatatg cccgacctgc agtatttcaa gctgtatctt tccgacaagg attataccgt
     1021 gcagcagtac ggcgcgcgcg atgaagtgat cggcgcgttc tatcaatacg gctccgaacg
     1081 cgcaacctat ctgccggaag agcaggaata acgcgcattt ttcggcggtt cggtcaagcg
     1141 ggccgccgga tgttttttag gcgacaggtt acgcccgggc gttgaagaac cgaccgaaat
     1201 tgggggattg tgagcgcgta acacaaactg ttcaatgtaa aataatacat ttttcatttt
     1261 gaaataacag tctgttcaca tatatattgt atgctttgat caatgaaaga actagatgtt
     1321 gaagaaagca atctttggca gaacaagatg atgtaggggg tgcgaaacat gctggatcgg
     1381 ttgtatttaa tcgcgtatct ttggagaacg aaatttcacc gccctgctgc gaaagcgagc
     1441 tgctgacgcg gttagaaaac gaaactgaac acccttgctt ttcgcaggtc agcgccgcga
     1501 gaagccgggg ttaaaccatt cctccggaca tttcgtccgg ggggtctttt tttattccat
     1561 tctagtcagg tttcacagta aaacacaaac gagggaaagt ggctttctgg tatgtggaaa
     1621 atttacttcg agaggttgct attgttccgt actgtgttaa aatgatcgga acggaacaag
     1681 gagcgaacat gaaagaaaga aacgtcggaa ttctctgcac ggcctcatgc tacgtccttt
     1741 ggggactgct gccgctgtac tggcatatgc tcgacgaagt ggatgcgttt gtcattctgg
     1801 ccaaccgtat cgtctggtcg gcggtcttta cgatcgtact cttgcttgcg ctccggcgct
     1861 ttggcgacgt caaggccgtc ctgcgcgacc gggcgaaaat gcggtttgtc atccccgccg
     1921 cggtcatgat tacgatcaat tgggggctct atatctgggc ggtcaacgcg gggcatctgc
     1981 tggacgcaag ccttgggtat tatctcaacc ctctgatggt gtttgcagtt ggaatgctgc
     2041 tgtttcgcga aaagtgcggc gcgctggact gggttgcgct tgcgctggcg acggtcggcg
     2101 tgctggtggc aacgctggcg tacggagcgt tcccctgggt ggcgatttcc cttgcgcttt
     2161 cgtttgcgct gtacggcacg tttaagaagc ttggcggcgt cagcgggctg acaagcatcg
     2221 cggtggaaac cattctcgtt gcgccgtttg cgctgggctt tttgctgctc tctcctgcga
     2281 gccacgcggc gttttccgcg ttaacgctca aaacggggct tttgctgttc tttacgggcg
     2341 ttgtcaccgc aactccgctg attctcttta ctcatggcgt caaccgcctg ccatttacca
     2401 cagtcggctt tctgcagtac atcagcccga cgctgcagct tatcatcgga acgctgtttt
     2461 tccatgaaac gctgacccag gaccgcgtcg ttgcgcttgt gttttttggc gtagcgctcg
     2521 tgctctattc cattggcata acgcggcgcg ccaagaagca gagcgcgctc gaaaccggcg
     2581 gtttaacccg ttagcgctcc tactgccgca ggtgcgccat aataacacgc gctttgtgaa
     2641 acccccgtgt attcgggggc tttttgttgc gatttatgcg gttttctgct atactccaac
     2701 cttagggtat tatgcggacg aatgcctttt ttaacgtcgc ttatgcagtg aaaacagttg
     2761 cgtttcgacg gggatttttg aaaggaatcg cactcgccca cggaaaagta aaccaaagga
     2821 gcttccgaat gggattaatc gatactctgc tcggcaatac gagcgcaagc cggctgaaaa
     2881 aactgcagcc gtatatcaag cgcatcaacg agatggaacc gcaaatgcag gcgatgacgg
     2941 atgaacagct ccgcgcgcag acgcagaagt tccgtgaacg cctcaaaaac ggcgaaacgc
     3001 tggacgacat catggtggaa gcgtttgcta ccgtgcgcga ggcggcggtg cggacggtgg
     3061 acatgcggca tttcgacgtg cagctgctcg gcggcatggt tttgcaccag ggccgtattt
     3121 ccgaaatgaa aacaggcgaa ggcaaaacgc tggttgcgac gcttccggcg tacttaaacg
     3181 cgctcacggg cgaaggcgtc catgtggtca ccgtcaacga ctatctggcc aagcgcgacg
     3241 cgcagtggat gggcaagata taccgcttcc tcggcatgag cgtcggctgc gtgatcaacg
     3301 gcatggaacg tgatgcaaag caggaagcct accgcagcga catcacctac ggcacgaaca
     3361 acgagttcgg cttcgactat ctgcgcgaca acatggtcgt tttccgcgag cagatggtgc
     3421 agcgcgagct ggcgtatgcc atcgtggacg aagtcgactc catcctcatc gatgaagcgc
     3481 gtacgccgct cattatcagc ggcgaaggcg aagagtccag cgagatgtat atcgaggcca
     3541 accgctttgt gcgcgggctc acgcgcggct ccaacctcac ccagcagtcc agttccgacc
     3601 ggattatggg cgaagagatt ccggaggacg gggactacca gtgcgacatt aaaaagcgca
     3661 ccgttcagct taccgccgag ggcatccgca aggccgaaga gcgttttagt ttagagaacc
     3721 tcggcgatcc ggccaatatg gagatcaacc accatatcgt gcaggcgctg cgcgccaacg
     3781 cgctgttcga gcgcgacaag gattatgtcg tgcaggacgg cgaagtactt atcgtagacg
     3841 agttcacggg tcgtctcatg atcggccgcc gctacagcga aggcctgcat caggcgctgg
     3901 aagccaagga aggcgttacc gttgaaaagg aaaaccggac gctcgcgacc attacgctgc
     3961 agaactattt ccgtatgtat aaaaagctct cgggcatgac cggtacggca aagaccgaag
     4021 aatccgagtt catgagcatt tacgatctgg acgtcgtaga gattccgacc aacaggccgc
     4081 tgatgcgcac gaatctggac gacgcgatct tccccaccga aaaaggcaag ttcgacagca
     4141 tcgttgaagc gatcaaggag attcacaaaa ccggccagcc ggtgttggtt ggtaccatca
     4201 gcgttgaaag aagcgaatac ctcagcgggc gtttgacccg cgctggcatc ccgcacgacg
     4261 tgttgaacgc aaagcagcat gcgcgcgagg cggaaattgt cgcccaggcc ggtaaaaaag
     4321 gtcaggtgac cattgccacc aacatggcag gccgcggcac ggacattctg ctcggcggca
     4381 acccggactt cctcgcccgg cgcgacctgc gtcaggacgg gctggaggac tggctcattg
     4441 aggaagccgt aggccatggg gaaacggctg atcccgatat cctcgcggct cgcgcccgct
     4501 ataaagagct ctacgacgcg cataaggttg ttacgtccaa ggaacacgac gaggtggaag
     4561 ctctcggcgg cctgttcatt ctcggcacgg agcggcatga gagccgccgc atcgataacc
     4621 agctgcgcgg acgcgcgggc cgccaaggcg accgcggcag cacgcagttc tacgtttcgt
     4681 ttgaggacga gctcatgcgc cggttcggcg gcgatcgcgt gaccaacacg cttaaccggt
     4741 ttgcttcccc cgagatgcag caggagatcc gcctcgaaat gcggatgctc acaaagcaga
     4801 tcgaagccgc gcagaagcgc gtggaa
//
LOCUS       DAOMGY010000024         4818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_17979_length_4818_cov_3.732312, whole genome shotgun sequence.
ACCESSION   DAOMGY010000024 DAOMGY010000000
VERSION     DAOMGY010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17979_length_4818_cov_3.732312"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PLR57_00755"
     CDS             <1..809
                     /locus_tag="PLR57_00755"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40013.1"
                     /translation="AILMYAVFYIPVAVMIAFSFNDAQRNYYWQGFTTDWYGKLFSFA
                     NTDLWDALLYSVIIAVLATIISVTIGVLGGIGLKKFRFVGKKFIDMMIYVPIIVPEIV
                     LAVAMLIIFMTIGVKLGMGTILIGHCTFCIPYAVVTIKGRISGDNETLQEASMDLGAN
                     RFQTFWKVTVPSIMPGIMSAAFLSFTLSIDDVVMSNMLAGARQSTLPVLILSLNRTGI
                     TPDANALTTVMIVALVLGMLAVNGLQRHRRKKEARLAAVAEKAISTAGGR"
     gene            847..1896
                     /locus_tag="PLR57_00760"
     CDS             847..1896
                     /locus_tag="PLR57_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HQQ40014.1"
                     /translation="MKKVLILVVVLAMLLTMLACGGKPAADQASTELNIFMWGDYISE
                     KLIADFEAANNCKVNLSYMSDNADSITKLTAGAGQDYDLIMTCDAYMESLIAGDYLEK
                     LDPAQIPNAVANINGAYWSGDNRDYCVPYLMNYIYIVYDKETCPIEITCYNDLIKPEM
                     AGQIGSIDGARNLFPMALIALGYDPNSTVESEIAEAYEWLVKYNANVVAYGATQENIV
                     NGTVSCMLTYDGNASWAMSQLGENNTLTIAPFTADPVQLGFDLYVIPKGATHMDLAYK
                     FLDYICVPEVMAENLVEFPYSCPNDAAVAAASDAYRNDPAFDFDYKTNIFFQEDVGDA
                     LTIYDKYYQMLKVGE"
     gene            1987..2982
                     /locus_tag="PLR57_00765"
     CDS             1987..2982
                     /locus_tag="PLR57_00765"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003732006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQQ40015.1"
                     /translation="MLKFDEEKFLDNLSGMLALRGRIERIVDAAAKAGVTNICWLGIG
                     GTWASCLQAVVHMKERSALEVFALNAAEYMATGDKRVGPGTFTVISSVTGTTAEMVEA
                     VKKARSTGAQVLGFIDKEDAELVSDVDFCISYPHNEQLKFFMTADRFMFDAGEFEDYA
                     EYYAQLDAHLPRLLADIEKQADGFGARFAEKHLHDSLHYFVGAGTLYGATYSYAMCYW
                     EEMHWMRTKSIHASEFFHGMLEIVDKDTPVTVFVGEDSQRELGLRVARFLPKVCENFT
                     VIDTRDYALPGIDEKYRGDLCHLIMHAVTNRIDAHIEALSGHDMTLRRYYRKLEY"
     gene            2990..3802
                     /gene="frlC"
                     /locus_tag="PLR57_00770"
     CDS             2990..3802
                     /gene="frlC"
                     /locus_tag="PLR57_00770"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HQQ40016.1"
                     /translation="MEYGLFTSGYQRVGLERAFSDAAAFGYDFIELWGGRPHAYAPDL
                     LSGGLAELRRLMERYAMPVRVYTPEHNAYPYNYMLGSDTQWADCVSYLSDAIRAGASL
                     GAEYTLISVGHGGGAPYPVRWQRLLKTLHALSRAAEDAGHKLLLETLTPYESNTCTTL
                     SELMLALREIESPCLFGMCDLVAPFTQNEDPADYVRKLGNNMRHLHLVDSDGVSDTHL
                     IPGDGAMPLASILRDMRAAGYDGTATIELVTNYIDRPSEAALLALSRAKELL"
     gene            3799..4587
                     /gene="frlD"
                     /locus_tag="PLR57_00775"
     CDS             3799..4587
                     /gene="frlD"
                     /locus_tag="PLR57_00775"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HQQ40017.1"
                     /translation="MSRIACVGDNCIDFYEQTGGAFPGGNPVNVAVYTCRLGGEASYT
                     GAVGTDAYGAFLVDALRQKGVDVSHVQVREGKTALTQVSMENGDRVLGDYDEGVMADF
                     SLSNEDIDFLCTHELVISGLWGRAENDFAAISRRGVLTAFDCADRPEDAAARAALPHT
                     DIAFFSDDRPDEAALKQRIRAIFAMGPRLVVATRGAAGSLAFDGEWFYSFGIIPCTVV
                     DTMGAGDSYIAGFLQAYLNKQSVPACMEAGARASVETLGYFGAW"
BASE COUNT          958 a         1426 c         1440 g          994 t
ORIGIN      
        1 acgcaatcct catgtacgcc gtgttctata tccccgtcgc ggtgatgatc gcgttctcgt
       61 tcaacgacgc gcagcgcaac tactactggc agggttttac gaccgattgg tacggcaagc
      121 tgttttcctt cgccaatacg gacctgtggg atgcgctgct ctactcggtg attattgcgg
      181 tgctcgccac catcatcagc gttacgatcg gcgtgctcgg cggcatcggg ctcaagaagt
      241 tccgctttgt gggcaagaag ttcatcgaca tgatgatcta cgtgcccatc atcgtgccgg
      301 agatcgtgct cgcggtggcg atgctgatca tctttatgac catcggcgtc aagctcggca
      361 tgggcacgat actcatcggc cactgcacct tctgcattcc ctacgccgtg gtgacgatca
      421 agggccgcat cagcggcgac aacgagacgc tgcaggaggc ctcgatggac ctcggcgcga
      481 accgcttcca gaccttctgg aaggtgacgg tgccgagcat tatgcccggc atcatgtcgg
      541 cggccttcct ctcgttcacg ctgtccatcg acgacgtggt tatgtccaac atgcttgcgg
      601 gcgcacggca gtccacgctg ccggtgctca tactttccct caaccgcacg ggcatcacgc
      661 ccgatgcaaa cgccttaacg accgtcatga tcgtggcgct ggtgctgggt atgctcgcgg
      721 tcaacggtct ccagcggcac cggcgaaaaa aggaggccag gctcgcggcc gtggccgaga
      781 aagcgatctc aactgccggg ggcagatgat aataagcgca ccaaaggaaa agaaggagga
      841 aatgaaatga aaaaggtact gatactggtg gtcgtgctgg ccatgctgct gaccatgctc
      901 gcctgtggcg gcaaacccgc cgcggaccag gcaagcacgg agctcaacat cttcatgtgg
      961 ggcgattaca tctccgaaaa gctgatcgcg gactttgagg cggccaacaa ttgcaaggtc
     1021 aacctcagct acatgtccga caatgccgat tccattacaa agctcaccgc gggcgccggt
     1081 caggactatg acctgatcat gacctgtgac gcgtacatgg agtccctgat cgcgggcgac
     1141 tatctcgaaa agctcgatcc tgcgcagatt cccaacgccg tggcgaatat caacggcgcc
     1201 tactggagcg gcgacaaccg cgactactgc gtcccctacc tgatgaacta tatctacatc
     1261 gtatatgaca aggagacctg cccgatcgag atcacctgct ataacgatct catcaagccc
     1321 gagatggccg gccagatcgg ttccatcgac ggcgcgcgca acctcttccc gatggcgctg
     1381 atcgcgctcg gctacgaccc caactccacc gtcgagtccg agatcgccga ggcctatgag
     1441 tggctcgtca agtacaacgc aaacgtcgtt gcctacggcg cgacgcagga gaacatcgtc
     1501 aacggcaccg tgtcctgcat gctcacctat gacggcaacg catcctgggc catgagccag
     1561 cttggcgaga acaatacgct caccatcgcg ccgtttaccg ccgatcccgt gcagctcggc
     1621 ttcgacctgt acgttatccc caagggagca acgcatatgg accttgcgta taagttcctc
     1681 gattacatct gcgtgcccga ggtcatggcc gagaacctcg tggagttccc gtactcctgc
     1741 cccaatgacg ccgccgtggc tgccgcgagc gacgcctacc gcaacgaccc ggccttcgac
     1801 ttcgattaca agaccaacat cttcttccag gaggatgtcg gcgacgcgct gaccatctac
     1861 gacaagtact atcagatgct caaggtcggc gaataacatt cctttgtaag cggcgcggcc
     1921 gctaaaagac cgtatgcggc cggagccccc tctccggccg catacaaaaa cgggaacgag
     1981 gttcttatgc tgaagtttga tgaagaaaaa tttctggaca atctaagcgg tatgctcgcg
     2041 cttcgcgggc gcattgagcg catcgtggac gccgccgcca aggcgggcgt gaccaacatc
     2101 tgctggctcg gcatcggcgg cacctgggca agctgcttgc aggccgtggt gcatatgaag
     2161 gagcgcagcg cgctcgaggt gttcgccctg aacgccgcgg aatacatggc taccggcgat
     2221 aagcgcgtcg ggcccggcac gttcacggtg atttcctccg tgaccggcac gacggcggag
     2281 atggtggagg ccgtgaagaa ggcgcgttcc acgggcgcgc aggtgcttgg ctttatcgac
     2341 aaggaggacg cggagctcgt gtccgacgtc gatttttgca tcagctaccc ccataacgag
     2401 cagctcaaat tctttatgac cgccgaccgc tttatgtttg acgccggcga atttgaggat
     2461 tatgcggaat attacgccca gcttgacgcg catctgccgc ggctgctggc cgatatagaa
     2521 aaacaggccg acggtttcgg cgcgcgcttt gcggaaaaac acctccacga cagcctgcac
     2581 tatttcgtgg gcgcgggtac gctctacggc gcgacctatt cctacgccat gtgctattgg
     2641 gaggagatgc attggatgcg cacaaagtcc atccatgctt cggagttttt tcatggcatg
     2701 ctggagatcg tggataagga tacgccggtg accgtgtttg tgggcgagga ttcccagcgt
     2761 gagctgggcc ttcgggtagc gcggttcctg cccaaggtct gcgagaattt tacggtcatc
     2821 gacacgcgcg attacgcgct gccgggcatc gatgaaaaat accgcggaga cctctgccat
     2881 ctaatcatgc acgccgttac gaaccgcatc gacgcgcata tcgaggccct gtccggccac
     2941 gacatgactc tccggcgcta ctaccggaag ctggaatact gagcgcgtca tggaatacgg
     3001 gctgtttacc tcgggctatc agcgcgtcgg gctcgaacgg gccttttcgg acgcggcggc
     3061 cttcggctat gactttatcg agctctgggg cgggcggccc cacgcctatg cgccggatct
     3121 gctttccggc gggcttgcgg aactgcggcg gctgatggaa agatatgcca tgcccgtgcg
     3181 cgtctatacg cccgagcata acgcctatcc gtacaactat atgctgggca gcgacacgca
     3241 gtgggcggat tgtgtgagct atctttccga tgcaattcgc gcgggcgcgt ccctcggcgc
     3301 cgaatacacg ctcatatccg tcggccatgg cggcggcgcg ccctatcccg tgcgttggca
     3361 gaggctcctt aagacgctgc acgcgctttc aagggcggcg gaggacgccg ggcataagct
     3421 gcttttagag acgctcacgc cctatgaatc caacacctgc acgacgcttt ctgagctcat
     3481 gctggcgctg cgcgagatcg aatcgccctg ccttttcggc atgtgcgatc tggtcgcgcc
     3541 gtttacgcag aacgaggacc ccgcggatta cgtgcgaaag ctcgggaata acatgcggca
     3601 tctgcatctc gtcgattccg acggcgtgag cgatacgcac cttatcccgg gcgacggtgc
     3661 gatgccgctt gcttccatct tgcgcgatat gcgcgcggcg ggttatgatg gaaccgcaac
     3721 gatcgagctg gtgacgaact atatcgacag gccctccgag gccgccttac tggcgctctc
     3781 gcgggcgaaa gagctgctat gagtaggatt gcctgtgtcg gcgataactg catcgatttt
     3841 tatgaacaga cgggcggtgc cttccccggc gggaaccccg taaacgtcgc tgtctacacc
     3901 tgccgcctgg gcggcgaggc ctcctatacc ggcgcggtcg gtacggatgc atacggcgcg
     3961 tttctggtgg atgcgctgcg ccaaaagggt gtggatgtga gccatgtgca ggtgcgcgag
     4021 ggaaagaccg cgctcacgca ggtcagcatg gaaaacggcg accgcgtcct cggcgattac
     4081 gatgagggcg tgatggcgga tttttcgctt tcaaacgagg atatcgactt tttgtgtacg
     4141 cacgagctcg tgatatcggg cctgtggggc cgggcggaaa atgattttgc cgccatttcg
     4201 cggcggggcg tgctgactgc tttcgactgt gcggacaggc ccgaggacgc cgccgcgcga
     4261 gcggcgctgc cacacacgga tatcgcgttt ttttccgacg acagaccgga tgaagcggcg
     4321 ctcaagcagc gcatacgcgc tatctttgcc atgggcccgc gcctcgtcgt cgcgacgcgg
     4381 ggcgccgcag ggagcctcgc tttcgacggc gagtggtttt attccttcgg tatcatcccc
     4441 tgcacagtcg tcgataccat gggcgccggc gacagctata tcgcaggatt tttacaggca
     4501 tatctgaaca agcaaagcgt tcccgcctgt atggaggccg gcgcgcgcgc gagcgtcgaa
     4561 acgctcggct atttcggcgc gtggtgactg tattaccggc tgccggtgcg tctgcctttt
     4621 cgtcccggca tttccctcaa atgatgaacg gcttttaaga tatacttttt gaaaacactt
     4681 attgtatgga ttgatctgcc cccgaattct aggccggaaa ctttgttagt aagcagtggt
     4741 tctaagcagc aatggggaca atcgtttgag gggcgggcgg ccgcccgccg aggctcgaat
     4801 gcggtctgac ctgattgt
//
LOCUS       DAOMGY010000025         4816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_17998_length_4816_cov_3.242176, whole genome shotgun sequence.
ACCESSION   DAOMGY010000025 DAOMGY010000000
VERSION     DAOMGY010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17998_length_4816_cov_3.242176"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..327)
                     /locus_tag="PLR57_00780"
     CDS             complement(73..327)
                     /locus_tag="PLR57_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40018.1"
                     /translation="MQEWDVVGVIAALFALFSAMVAPMIKLTRAITRLTTTMENMEKS
                     VENLTTNNQGAHERLWQHEREQDDKLCDHESRLRVIEDDR"
     gene            complement(343..1236)
                     /locus_tag="PLR57_00785"
     CDS             complement(343..1236)
                     /locus_tag="PLR57_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HQQ40019.1"
                     /translation="MELTRNLRCGMAGEDVACIKAKLFALGMYTPNITAVTSDRFGED
                     TKRAVKAFQAEKGLAADGVVGPLTFAALFGTISELESTASADSAELPANIGQAAARAI
                     AEELAGASEVRRSIVLDALRFAYDPAVPAPYPLSLYIRGGNLYNTDLQPNVITLERIA
                     SGAKRQPEFYDGGRREMMERAVRANPSITGADCSGGVVGLLRHARVVSKGFDLSANGF
                     FASASCKKIDQSALQPADLLHKSGHIGLYAGGGYAVEWMGGAYGCQLTRLSARRGWNF
                     VKSKLDKSGDWTGFLQPGYYK"
     gene            complement(1515..1943)
                     /locus_tag="PLR57_00790"
     CDS             complement(1515..1943)
                     /locus_tag="PLR57_00790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ40020.1"
                     /translation="MPQDGELTLRIKLISLADEKTGYVPMYRFDIVRLSDGACVGATD
                     LRLGYVRNTFYGGNIGYSVEEPYRGHAYAQKAVRLVFEVARAHGMPYLIITCSEANEP
                     SRKTLEHLGGKLLETHVPPSYSALYKEGVHDSCCIFRFDL"
     gene            complement(1995..2459)
                     /locus_tag="PLR57_00795"
                     /pseudo
     CDS             complement(1995..2459)
                     /locus_tag="PLR57_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394158.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
     gene            complement(2562..3728)
                     /locus_tag="PLR57_00800"
     CDS             complement(2562..3728)
                     /locus_tag="PLR57_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQQ40021.1"
                     /translation="MEQVLKRHRSIWFFLKATLGGIIKNIYGFECEPADPGKGPYLIL
                     ANHNTDLDPALIALSFPDHMYFVASEHVYRKGFVSWLLKYCFAPISRTKGTTDATAAL
                     NVIRTIRQKSNVCLFAEGNRSFNGVTGPIFPATGKLAKATGASLVTYRFEGGYLTLPR
                     WARSIRKGRMVGRLVHVYTPEELKAMTPEEVNRHIREDIAEDAFDRQCMQPHPLRYRG
                     KGLAEGLENALYFCPKCGKPGTLHSKDDLFFCDCGMSVRYTETGFFEGEDVPFSTVRD
                     WDTWQNGRLKEYIDSLGDDEIAFSDENVKLIGVELGHKSRLLETGKLFITRTVIGVGA
                     HSFPLEKVVSMGLMGTYKMMFSVEGVSYELRSAKNMYCGRKYLTFFQTLYPDRL"
     gene            complement(3866..4552)
                     /locus_tag="PLR57_00805"
     CDS             complement(3866..4552)
                     /locus_tag="PLR57_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ40022.1"
                     /translation="MFLFSMIPAVFGLIILSFVREQKQARVKKTEAHPKVSWKSLDLR
                     LRLFLITAFIFNLGNSSNTFLLLRSYDVGYDAVSVILLYLLYNVVSSALCLPMGKLSD
                     RIGKKRLLVFGYLAFSLTYLGFAVAKSGIAFLLLFVLYGVYTAMTAGVERALIAVLAP
                     SEHKGAVLGLHGTLTGIALLPASVIAGLLWNNVSASAPFFLGAALSFVAVVAIALIFR
                     RGGESSAQIV"
     gene            4569..>4816
                     /locus_tag="PLR57_00810"
     CDS             4569..>4816
                     /locus_tag="PLR57_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HQQ40023.1"
                     /translation="MPALEADPYYAWIVAAFAPLQYAAFVMYAWAAARGGLGPVEMLG
                     LALSAGFMNGVGIANAHELGHKPTRRERWLAKIVLAPTA"
BASE COUNT          990 a         1470 c         1298 g         1058 t
ORIGIN      
        1 aatccagcaa ctgttccata tcgattctcc tttaattttt cgttccaaat ttggattcat
       61 cgattcgcgt attcaacggt cgtcttcaat gacccgcaag cgcgattcgt ggtcgcagag
      121 tttatcgtcc tgctcccgtt cgtgctgcca gagcctttca tgcgcgccct ggttgttggt
      181 cgtcaggttt tctacgctct tttccatatt ctccatggtt gtcgtcaagc gcgtaatcgc
      241 gcgcgtgagt ttgatcatgg gggcgaccat cgcggaaaag agcgcgaaca gcgcggcaat
      301 cacgccgaca acgtcccatt cctgcatgct ctttttccct ttctacttgt agtaacccgg
      361 ctgcaggaat cccgtccagt cgccgctctt gtccagtttg ctcttaacaa agttccatcc
      421 gcgccgggcg gacaggcgcg tgagttggca gccgtatgcg ccgcccatcc actctacggc
      481 gtacccgccg cccgcataca gcccgatatg cccgctttta tgcagcagat cggcgggctg
      541 aagcgcgctt tgatcgatct tcttgcatga cgcgcttgca aagaatccgt ttgccgaaag
      601 gtcgaaccct ttcgagacga cccgcgcatg ccgcagaagg cctaccacgc cgccggagca
      661 gtccgcgccc gtgatcgagg gatttgcccg caccgcgcgc tccatcatct cccgccgccc
      721 gccgtcgtag aactccggct gacgctttgc gccggaagcg attcgctcca gcgtaatcac
      781 gttcggctgc agatcggtgt tgtagagatt cccgccacgg atatacagac tcagcggata
      841 cggcgccgga acggctggat cgtaggcgaa ccggagcgcg tccagcacga tcgaccgccg
      901 gacttcgctt gcacccgcga gctcttctgc aatcgcgcgc gccgccgcct gcccgatgtt
      961 tgccggaagc tccgcgctgt ctgccgaagc ggtgctctcc agttcgctta tcgtgccgaa
     1021 cagcgcagca aacgtcagcg ggcctacaac gccgtcagcc gcgagtcctt tctccgcctg
     1081 aaaggctttg accgcacgtt tggtgtcctc gccgaagcgg tcgctcgtca cagctgtaat
     1141 attcggcgtg tacatgccga gcgcaaagag ctttgctttg atacatgcta cgtcctcgcc
     1201 cgccatgccg cagcggaggt ttcgcgtcag ttccatgcgc ggctccgttt ctcagtttcc
     1261 ggcttcccga agccgaaagc ggacgcgcca cggttctgca caccagacag cggcaagacg
     1321 gcagatgcgt tttggttggg catttttctc ctgtggggta tccaaccata ttgttgcacg
     1381 gtttttttgc ggattttacg acaataaaaa ttctaataat gaatcagcaa aaaaagacgg
     1441 attgctccgt ctttcgcact aaaatagtct tgtttttcta atgtttttta agaattgtgc
     1501 tctatttatt tgtgttacag gtcaaaccgg aaaatgcagc acgaatcgtg aacgccttcc
     1561 ttatacaagg cgctgtagct cggcggaacg tgcgtttcca gcagttttcc acccagatgc
     1621 tccagcgtct tgcgggaagg ttcgtttgct tccgagcacg taatgatcag atacggcatt
     1681 ccgtgcgcgc gggctacttc aaacacgagc cgcacagcct tctgcgcgta agcatgccca
     1741 cgatacggtt cttccacgct gtagccgata ttgccgccgt aaaaggtgtt tcgaacgtac
     1801 ccgagccgca aatccgttgc gccgacgcaa gccccgtcgc tgaggcgcac gatatcgaag
     1861 cggtacatcg gcacataccc cgtcttttcg tccgcaagtg aaatcaactt gatgcggagc
     1921 gtgagctcgc cgtcctgcgg catctccatc cccggtttcg gctcaaagtc tatcacaaga
     1981 tcgtttcccg gttcccagtc cttgcagacg tccacccaac ccgccgcgtt gctataccga
     2041 aacagctcgt cgctgctgag ttcaatagcg ctgtttccgc ttccgcaggc gggaaacacg
     2101 gttgtaaagc gtttgagcga ctcgtcgaga tagaccggca cgccctccgg caaggcaaac
     2161 gggcaaacgc cgcccactgc gctgccggtc aggcgctcca cctcgtcgaa ggcgagcatg
     2221 cgcgctttcg ttttgaactg ctccttgaac ttcgggttgt cgatgcgcgc gtcccctgag
     2281 gcaaccacca gaacgcaccc ttcgcccagc gcgaacgaaa gcgttttggc gattctagca
     2341 gggcgtacgt ccagcgtctc ggccgcgagt tccaccgttg cggacgaggt gtcgaactcc
     2401 caaacgcgct gatccatacc gagcgatttg aagtaatcct tcactttttc aactgacata
     2461 acgggtatct tcctttgcct gtaacgaagc cgccggtact tcggcggctt ctgaattcaa
     2521 ataaaatatg attgtacgcg gctctctgtt tcctgacgcg attacaggcg gtcgggatag
     2581 agcgtctgga agaaggtaag gtacttcctg ccgcaataca tgttctttgc tgagcgtagt
     2641 tcgtaggaaa cgccttccac ggagaacatc atcttgtatg tgcccataag tcccatgctg
     2701 accacttttt ccagcgggaa gctgtgcgcc cctactccga tgacggtcct ggtgataaag
     2761 agctttcccg tttccagcag gcggctctta tgccccagct ctacgccgat gagcttgacg
     2821 ttctcgtccg aaaacgcgat ttcgtcgtcg ccaagcgaat cgatgtactc tttcaggcgg
     2881 ccgttctgcc aggtatccca atcccgcacc gtggaaaacg gaacgtcctc gccctcaaag
     2941 aagccggttt cggtgtagcg cacgctcatg ccgcagtcgc agaagaaaag gtcgtcctta
     3001 ctgtgcagcg tgccgggctt cccgcatttg gggcagaagt agagcgcgtt ttccagccct
     3061 tcggcgagcc ctttgccgcg gtaacgcagc ggatgcggct gcatgcactg ccggtcaaac
     3121 gcgtcctcgg ctatatcctc gcggatatgg cggttcactt cctccggcgt cattgctttg
     3181 agttcttccg gcgtgtagac atgcacaagc ctgcctacca tgcgtccctt gcgaatcgag
     3241 cgcgcccagc gcggcagggt caggtagccg ccctcaaagc ggtaggtcac cagcgaagcg
     3301 cccgtcgctt ttgcaagttt gcccgtcgcc gggaaaatcg gcccggtaac gccgttaaac
     3361 gagcggttgc cttccgcaaa caggcagacg ttgctcttct ggcgaattgt gcgtataacg
     3421 ttcagcgccg ccgttgcgtc cgtcgtgcct ttcgtgcggg aaatcggcgc gaagcagtat
     3481 ttaagcagcc aggagacaaa tcccttgcgg tacacgtgct cactggcaac aaaatacata
     3541 tgatccggaa aactaagcgc aatcagcgcg ggatccaggt cggtgttgtg attagccagt
     3601 atcagatacg gtcccttgcc ggggtccgcg ggttcgcact caaatccgta gatattctta
     3661 atgatgccgc cgagcgtcgc tttcaaaaaa aaccaaatgc tgcggtgccg tttcaatact
     3721 tgttccaaat ctgctcacct gcctgtctgt tttgattgcc tgtagagtcc tgcttcatcg
     3781 ccggtaaacc cggcgttctg ctgactctat tatactatgc ggtttttacc gcgggcaact
     3841 caagcgccgc cggtaaaagc gcatttcaga caatctgcgc cgatgattcg ccgccgcgcc
     3901 ggaatatcag cgcaatggcg acgacggcta caaacgacag cgcggctcca agaaagaacg
     3961 gcgcggacgc ggaaacattg ttccaaagaa gtcccgcaat caccgaagcg ggcagcagcg
     4021 caatcccggt cagcgttccg tgcaggccga gaacggctcc cttgtgctcc gatggtgcga
     4081 gcaccgcgat cagcgcgcgc tccacgcccg ccgtcatggc ggtatacacg ccgtacaaca
     4141 caaacaggag caaaaacgca attccgctct ttgccacggc gaacccaagg tatgtcagcg
     4201 aaaacgcgag ataaccgaaa acaagcagcc gctttttgcc gatcctgtcg gaaagcttcc
     4261 ccatcggcag gcaaagcgcg gagctcacca cgttgtagag caggtacaag agaatcacgc
     4321 tgacagcgtc gtagcctacg tcgtaggatc tcagcagcag aaacgtgttg gaggagttgc
     4381 ccaggttaaa gatgaacgcc gtaatcaaaa acagccgcag gcgaagatcc agcgacttcc
     4441 aggaaacttt ggggtgcgct tcggttttct tcacgcgcgc ctgcttctgt tcgcgaacaa
     4501 acgagagaat aatcagcccg aaaaccgcag gtatcatcga aaagagaaac acacgctcat
     4561 aatccgccgt gcccgcgctt gaggccgatc cctattacgc ctggatcgtc gcggccttcg
     4621 cgccgctgca atacgccgct ttcgtcatgt atgcgtgggc ggcggcgcgc ggcggactgg
     4681 ggccggtcga aatgctcggc ctcgcgctca gcgccggctt catgaacggc gtcggcatcg
     4741 ccaacgcgca cgaactcgga cacaagccga cgcggcggga gcgttggctc gcgaaaatcg
     4801 tgctggcgcc gaccgc
//
LOCUS       DAOMGY010000026         4814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_18007_length_4814_cov_3.165371, whole genome shotgun sequence.
ACCESSION   DAOMGY010000026 DAOMGY010000000
VERSION     DAOMGY010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18007_length_4814_cov_3.165371"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1304
                     /locus_tag="PLR57_00815"
     CDS             333..1304
                     /locus_tag="PLR57_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ40024.1"
                     /translation="MLDQKQMVVLGGLPQKIHIKSENETNPVLLFLHGGPGVCNRHTI
                     MKDHADLAKVFTMVAWDQRGSGGSYWGAKQETLTVDQLVEDANELVGWLCETFHKDKI
                     FIIGGSWGSELGTYLAFRHPEKIAAYVGFGQVVNGAKNEEISFDFAMAEAVKAGDEKS
                     VEILKKVGPPVMGVYKGGFDGMMAQRRVMMKYGGYSQSAKKRSYFRSFVIPVVLSGEY
                     SLKDLIGLVKGYKYVLTHMWNAVGETDFPSTCTKFDVPYFVFDGVLDQNTPASLVQGW
                     FDSIVAPQKELIWFEQSGHNPMGDEPARFKSLLIDRLTVIAKKEKNV"
     gene            1301..2701
                     /locus_tag="PLR57_00820"
     CDS             1301..2701
                     /locus_tag="PLR57_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HQQ40025.1"
                     /translation="MNETAVQAIPRTRFSEKFGFFTFSTASNVVYQFKSIYYLFFLTN
                     VMMLDVRIAGLILTIGTIWDAINDPLIGFWAVNHKFKNGERVRPLAIWFAVPWAITVV
                     LLFTNFGLSNQLSIALALVIYLLFELFNTFVGIPYNSMGSLATNLDADRRSINVFRNL
                     GGALGGGIGAVACLPLLKLFGALDGKGNLIASTSSRGFLLTACVMGVVCIIGCLVHYF
                     TTKERVKPIGDDDEKLSVKTVFTMLFKCKSWVLNMCYIICYGVINLLLMSCLTYYATY
                     VIGSTSAATAIQAAYLAASIGSSFLVSAIDRAIGRKKTMFLAAVLFILGKVWFVINPF
                     SIGAIYVNAITVGIAVTITFVMFNTNRNNIVDLIEWQSGRRLDSLVSTADNLASKLAV
                     AGATWLVTAALSGSGYDAKLAVQPTAAINTINAALGWVPMFVAVVMLAVILNLNIEKE
                     TKKMLEEKAAQAAQSV"
     regulatory      2850..2936
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3107..3484
                     /gene="gcvH"
                     /locus_tag="PLR57_00825"
     CDS             3107..3484
                     /gene="gcvH"
                     /locus_tag="PLR57_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQQ40026.1"
                     /translation="MNLPNDRKYTASHEWLMDMGGGIYRVGLSDFAQHALGDIVFIDL
                     PAVGDKLTKDVSLGDVESVKAVSEVVSPVSGTVSAVNDALSGAPETINAAPYETWLVE
                     VTDATAFSDLLDAAAYQAVCEQA"
     gene            3577..>4814
                     /gene="gcvPA"
                     /locus_tag="PLR57_00830"
     CDS             3577..>4814
                     /gene="gcvPA"
                     /locus_tag="PLR57_00830"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HQQ40027.1"
                     /translation="MGDYLPNTEEQRMDMLSAIGVSSVSELYADIPASLRESAAKAPA
                     ELPKGVSEFEALEHLRALSKKNTVYDAIFRGAGAYWHYIPPVVSRVAAKEAFVTAYTP
                     YQPEISQGILQTIFEYQTMIAELTGMDASNASVYDGAVAAAEAASMCRERSRKKTLVS
                     LGLHPESVETVQTYCHGADETVETLPLKNGAVDLTALKAALDETVSGVVIQSPNFYGV
                     IEDVSAIAEIVHAAGAKLVVSTNPIACALLKTPGECGADVAIGEGQPLGMPLSFGGPY
                     LGFMACKKDMIRKLPGRIVGQTSDAQGRRCFVLTLQAREQHIRREKASSNICSNQALC
                     ALTAAAYCSAMGPQGITEVAKQCYDKAHALADGLGSVKGFELRYPNAPFFHEFVTKTP
                     VPAEQLLAALAKKGILGGLPV"
BASE COUNT         1074 a         1365 c         1364 g         1011 t
ORIGIN      
        1 tacggttgcc atttgctcct ccattttttc ggcatctatt ctcaatagcc atatcattgg
       61 cataatacgc cagtatcaat agatattaac acaaggaaaa cgtgattgca accgcgattc
      121 cggcgttatt tggggctttt tggtgtcttt tcgggagtcg gaagcgccat gaggcaatgc
      181 aaaaacggtt ccgccgcccg ttgattccgg cataaccact tttttattca aaaagcgcgt
      241 aatcactgga aattggcgcg gatttcgtat atcattatgt gaacttatat acaagtgttc
      301 attatccaac gctggaaaaa cgggaggtat gtatgctcga tcaaaagcaa atggtcgtac
      361 tcggcggatt gccgcagaag attcacatca aaagcgagaa cgaaacgaac cccgttctgc
      421 tgtttttgca cggagggccg ggcgtctgca accgccacac gatcatgaag gaccatgccg
      481 atcttgccaa ggtgtttacg atggttgcat gggatcagcg cggcagcggc ggctcctact
      541 ggggcgcaaa gcaggaaacg ctcaccgtcg atcaactggt ggaggacgca aacgagctgg
      601 tcggctggct ctgcgaaacg ttccataagg acaaaatctt catcatcggc ggaagctggg
      661 gcagcgagct cggcacgtat cttgccttcc ggcatccgga aaagattgcg gcttatgtgg
      721 gctttggcca ggtcgtcaac ggcgcgaaga acgaggagat cagcttcgac ttcgccatgg
      781 cggaagccgt caaagcgggg gatgaaaagt cggttgagat tctcaaaaaa gtcggccctc
      841 ctgtgatggg cgtttataaa ggcggtttcg acggcatgat ggcgcagcgc cgggttatga
      901 tgaaatacgg cggctattcg cagagcgcca agaagcggag ctacttccgc tcattcgtca
      961 tccccgtcgt gctctccggc gagtacagcc tgaaggattt aatcgggctg gtcaaaggct
     1021 ataaatacgt cctgacccat atgtggaacg cggttggcga aacggatttc ccaagtacct
     1081 gcaccaagtt cgacgtgccg tatttcgtgt tcgacggtgt tttggatcag aacacgcccg
     1141 cgtctctcgt gcagggctgg ttcgacagca tcgttgctcc gcagaaggaa ctgatctggt
     1201 ttgagcaatc cggccataac ccgatgggcg acgagcccgc gcggttcaag agcttattga
     1261 tcgaccgctt gacggtgatt gcaaaaaagg agaaaaacgt atgaacgaga cagccgtaca
     1321 ggccattccc cgcacgcgat tttccgaaaa attcggattt ttcacttttt cgaccgcaag
     1381 caacgtcgtc tatcagttta agagtattta ttacctattc ttcttaacca acgtgatgat
     1441 gctggacgtg cgcattgctg gactgatcct caccatcggc accatctggg atgcaatcaa
     1501 cgatccgctg atcggattct gggcggtcaa ccacaagttc aaaaacggcg aacgcgtccg
     1561 cccgctggcc atctggtttg cggtgccctg ggcgattacg gtcgtgctgc tgtttacgaa
     1621 tttcggtttg agcaatcaac tctccatcgc gcttgcgctg gtgatctacc tgctgtttga
     1681 gctgttcaac acgttcgtgg gcattcccta caacagcatg ggaagcttag ccaccaacct
     1741 cgacgccgac cgccgctcga tcaacgtttt ccgcaacctt ggcggcgcgc tcggcggagg
     1801 catcggcgcg gtggcctgcc tgccgctgtt aaagctcttt ggcgcgctgg acggcaaggg
     1861 caacctgatt gcctccacca gctcgcgcgg atttttgctc accgcttgcg taatgggcgt
     1921 agtctgcatc atcgggtgcc tggtgcatta ctttacgacc aaggagcgcg tcaagccgat
     1981 tggcgacgat gatgagaagc tctcggtcaa aacggtgttc acgatgctgt tcaagtgcaa
     2041 gtcgtgggtg ctgaacatgt gctatatcat ctgctacggc gtaatcaacc tgctgctgat
     2101 gagctgcctg acctactacg caacctacgt catcggcagt acgagcgcgg caaccgcaat
     2161 tcaagcggcg tatctggcgg cttcaatcgg ctcttcgttc ctcgtttcgg ccattgaccg
     2221 cgccatcggc agaaagaaga ccatgttcct cgccgccgtg ctctttatcc tcggcaaagt
     2281 ctggtttgtg ataaacccct tctcgattgg cgcaatctac gtcaatgcca tcacggtcgg
     2341 catcgcggtg acgatcacgt tcgtcatgtt caatacgaac cgcaacaaca tcgtcgatct
     2401 catcgagtgg cagtccggcc gccgtctcga cagtctcgtt tccacggcgg acaacctcgc
     2461 cagcaagctc gctgtcgcgg gcgcaacctg gctcgtgact gccgcgcttt ccggcagcgg
     2521 atacgacgca aagctcgccg tccagccgac agccgccatc aacaccatca acgcagcgct
     2581 cggctgggtg ccgatgttcg ttgcggtcgt gatgcttgcg gtcattctca acttgaacat
     2641 cgaaaaggaa accaagaaga tgctcgagga gaaggcggct caagcggcgc agtcagtata
     2701 agaagcatcc tcaaacgtaa atatttaggt ttccaaaggg agtattccct ttggcggggg
     2761 atcgggggtg gaacccccga aaatccctct tcttggttga caattgccgc aagaaacggt
     2821 atattgaacc cggacaattt catactcgga tgaaggcatc gggagagacc gcgaaagccg
     2881 cggcgccgaa ggggaatgcg caacaaactc tcaggcaaaa ggaccgatac ccgacgaagc
     2941 tccggaaagc cgcttggtaa aacaaacggc accgacgagg caattccacg cagtcagcta
     3001 aacggcggcg cgggagaatc tttcaggttc agctacgggg cgcgcgaagt ttattcgcgc
     3061 gtctcgtttt ttatttccta ataatttgat aacggaggaa gaacttatga atctcccgaa
     3121 cgaccgcaag tacacggctt cccacgaatg gcttatggac atgggcggcg gcatttatcg
     3181 cgtcggcctc agcgactttg cgcagcacgc gctgggcgac atcgtattca tcgacctgcc
     3241 cgccgtcggc gataaactga ccaaagacgt ttcccttggg gacgtcgaga gcgtcaaagc
     3301 cgtttcggaa gtggtcagcc ccgtgagcgg aaccgtttcc gccgtcaacg atgcgctctc
     3361 cggcgcgccg gagaccatca acgccgctcc ctacgaaacc tggcttgtcg aagtaacgga
     3421 cgcaaccgca ttttccgacc tgctggacgc agccgcctat caggccgtct gcgaacaggc
     3481 gtaaccaacg aacaacagcc tcccgcacgt ttgccggggg ctgtttgtct ttgtgccatc
     3541 aaactaactt cctcacagcc gaaaggaggg gttcccttgg gagattatct gcccaatacg
     3601 gaagaacagc gaatggacat gctgagcgcc atcggcgttt caagcgtgag cgagctgtat
     3661 gccgatattc ccgcgtccct gcgggagagc gcggcaaagg cccccgcaga actgccgaaa
     3721 ggcgtttcgg aattcgaagc gctggagcat ttaagagcct tgtctaaaaa gaacaccgtc
     3781 tacgacgcaa tctttcgcgg cgcgggcgcg tactggcact atatcccgcc cgtggtgagc
     3841 cgcgttgccg caaaggaagc gttcgtcacc gcgtatacgc cctatcagcc ggagatcagc
     3901 cagggcatcc tgcagacgat attcgaatac cagaccatga ttgccgaact caccggcatg
     3961 gacgcttcca acgcttctgt atacgacggc gcggtggcgg cggcggaagc ggcgagcatg
     4021 tgccgcgagc gcagccgtaa aaaaacgctc gtttcgctgg ggcttcaccc tgagtcggtc
     4081 gaaaccgtgc agacctactg ccacggcgcg gacgaaacgg ttgaaaccct gccgctgaaa
     4141 aacggcgcgg tcgatctgac cgcgctcaaa gccgcgctgg acgaaacggt gtccggcgtg
     4201 gtgattcagt cgcccaactt ttacggcgtg attgaggacg tttccgctat tgcggagatc
     4261 gtccacgcgg cgggcgcaaa actcgttgtg agcacaaacc ccatcgcctg cgcgctttta
     4321 aaaacgccgg gcgagtgcgg cgcggacgtc gccattggcg aaggccagcc gctgggcatg
     4381 ccgctgagct tcggcgggcc gtacctcggg tttatggcct gcaaaaagga catgatccgc
     4441 aaactgcccg gacgcatcgt cggccagact tccgatgcgc aaggacgcag atgctttgtg
     4501 ctcaccctgc aggcgcgcga acagcacatc cgccgggaaa aggcgagcag caacatctgc
     4561 tcgaatcagg cgctctgcgc gctcacggcg gcggcgtact gctccgccat ggggccgcag
     4621 gggataacgg aagttgcaaa acagtgctac gacaaggcgc acgcgcttgc ggacgggctt
     4681 ggttccgtca agggcttcga gctgcgctac cccaacgcgc cgttcttcca tgagtttgtg
     4741 accaagacgc ccgttcctgc ggaacagctt cttgccgcgc ttgccaagaa aggcattctc
     4801 ggcggcctgc ccgt
//
LOCUS       DAOMGY010000027         4790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_18145_length_4790_cov_3.580570, whole genome shotgun sequence.
ACCESSION   DAOMGY010000027 DAOMGY010000000
VERSION     DAOMGY010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18145_length_4790_cov_3.580570"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLR57_00835"
     CDS             <1..494
                     /locus_tag="PLR57_00835"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HQQ40028.1"
                     /translation="RPPGEYPMTGVTRPLPSWVLNLLGFGILIALVLAVFFWQLLDMD
                     RELLYRRIDQRVDLMIEHGLLAEARNIYDRGYNRSLPAMQSIGYRQLLEVFDGKSTLE
                     QAVEQIKLDTRHFAKRQLTWFRRDKRIRWSDASDFETIKKGLTQRSADLTMRWMDEKE
                     LFE"
     gene            491..1735
                     /locus_tag="PLR57_00840"
     CDS             491..1735
                     /locus_tag="PLR57_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HQQ40029.1"
                     /translation="MIEISDRARELVWSAESALTGVFQRTDAIEMANQQRVLEAFWAE
                     RVSARHFAPTTGYGYDDIGRDTLDRVFARALSAEAALVRPQFANGTHAIYTALASLVE
                     PGDRILSITGQPYDTLQTAIGLSGNASNSLKKMGVRFDAIELLPDGGIDLEAIRSADA
                     SSVNVIFAQRSRGYAWREALNMNKLSEAFALCRELFPNAVILVDNCYGEFVEALEPTA
                     CGADLIAGSLIKNPGGGLAPTGGYIAGKKELVERAAERLTVPGMGGEVGSYAGDYRLF
                     YQGLFLAPHVTAQCVKSAALFARVFETLELSTMPASDAVRSDIIQAVRFEDADSLVRF
                     CGAIQSSAPVDSFVSPEPWDMPGYDDPVVMAAGAFVQGATTELSADGPIRSPYTAYLQ
                     GALTYAHARIAAMLACDALQKE"
     gene            complement(1790..2425)
                     /gene="lexA"
                     /locus_tag="PLR57_00845"
     CDS             complement(1790..2425)
                     /gene="lexA"
                     /locus_tag="PLR57_00845"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HQQ40030.1"
                     /translation="MKKLSDPQQVILDYMISYADEHSYPPSVREICAAVGLKSTATVH
                     AHLQNLEKKGMITRDSSKQRAIFIHSENIPDRAEREEKKYAVPLVGHVAAGMPMLATE
                     NIEDAFPLPDVLMHGKEPEEVYMLRVEGESMRDAGIRDGDILVVNHNCSCTNGDIVVA
                     RVAGESATVKRFFREKDRVRLQPENDLFEPIYVAFNDLEISGKVIGLLRTI"
     gene            2560..2901
                     /locus_tag="PLR57_00850"
     CDS             2560..2901
                     /locus_tag="PLR57_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40031.1"
                     /translation="MKRCVLHAEKTCTNCGECDDRCELDPQKICDNCFRCLETDQKSF
                     LEIPVSGVYFEDDFTPEPVLPAELELDEDEVPVLDSDDSWREEFHLGVSTLNGATASR
                     AKRRHIRRYFV"
     gene            complement(2933..3979)
                     /locus_tag="PLR57_00855"
     CDS             complement(2933..3979)
                     /locus_tag="PLR57_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQQ40032.1"
                     /translation="MANTFQAEKFKDYTMKIRALLPELPLVCGSFFRAIESQTSVLTR
                     YAYAIDLRGFFQFASAQEDLFPGKPIPSLTASDLEKIKAYHIELFLNEVSFYQKGERE
                     LVNQEQAKARKLSSIRSFFKYLHKQEMLPNNVAALVDIPKLHEKAIIRLEPNEVADLL
                     DLTENGEGLSDRQKQYHSATKIRDLAILTLFLGTGIRISELVGIDLGDVDLENDAFVV
                     TRKGGSEDILSFGEEVHKALSDYLSWRSAQTPLPGHEQAMFLSIQMRRITARAVENLV
                     KKYAHLAAPLKKITPHKLRSTYGTMLYHETEDIYLVADVLGHKDVNTTRKHYAATSEE
                     HKRIAAKKIKLRDD"
     gene            complement(4113..4595)
                     /gene="rnhA"
                     /locus_tag="PLR57_00860"
     CDS             complement(4113..4595)
                     /gene="rnhA"
                     /locus_tag="PLR57_00860"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HQQ40033.1"
                     /translation="MKKKVTIYTDGACSGNPGPGGWGAILLYKDQTRELSGFEPDTTN
                     NRMELKAVIEAIRALKIPCEIAVHTDSAYVCNAFHNGWLENWSANGWRTVSKRPVENQ
                     DLWRALLDILKEHQFTFVKVKGHSDNEFNNRCDALARQAIKQNTAPQSQNGQTDQNPS
                     "
     gene            complement(4635..>4790)
                     /locus_tag="PLR57_00865"
     CDS             complement(4635..>4790)
                     /locus_tag="PLR57_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HQQ40034.1"
                     /translation="GVHPGELKDRVCSPGGTTIEAVAVLEQKGLRGTVIAAVDACREK
                     SEKMSKQ"
BASE COUNT         1042 a         1246 c         1352 g         1150 t
ORIGIN      
        1 gacgcccccc aggagaatac cccatgaccg gggtaactag accccttccc tcctgggttc
       61 tcaatctgct gggattcggg atactcattg ccctggtgct ggcggtcttc ttctggcaac
      121 tgctggatat ggatcgggaa ctgctgtatc gcaggattga tcaaagagtc gatctcatga
      181 tcgaacacgg gttgctggcc gaagcgagaa atatttacga ccgcggctac aacaggagtt
      241 tgcctgccat gcagtcgatc ggctacaggc agttgttaga ggtgtttgac ggaaaatcaa
      301 cgttggagca ggcggtcgaa cagattaagc tcgacacacg gcatttcgcc aaacggcagc
      361 taacctggtt tcggcgggat aagcgcatcc gctggagcga cgcaagcgat tttgaaacga
      421 taaaaaaagg tttgacgcaa aggtcggcgg acttaacaat gcgttggatg gacgaaaagg
      481 agttattcga atgatcgaga tttcagaccg tgctcgcgaa ctcgtctgga gcgcggagag
      541 cgcgctgacg ggcgtgtttc agcgtacgga cgcaatagaa atggcaaacc agcagcgcgt
      601 actggaagcg ttctgggcgg agcgagtgag cgcacggcat tttgcgccga ccaccggcta
      661 cggctacgac gatatcgggc gcgacacgct ggaccgcgtt tttgcgcgcg cgttatccgc
      721 agaagcagcg ctggtgcgcc cgcagtttgc aaacggcacg cacgcgattt ataccgcgct
      781 cgcgtcgctg gttgagccgg gggacaggat tctttcgatc acggggcagc cctacgacac
      841 gctccagact gcgattgggc tcagcggcaa cgcttcgaac tcactcaaga aaatgggcgt
      901 ccgttttgac gcaatcgagc ttctgccgga tggcggaatc gatttggaag cgatccgaag
      961 tgcagatgcg agttctgtta atgtaatttt cgctcagcgg tcgcgcgggt atgcctggcg
     1021 cgaagcgctg aacatgaaca agctttcgga agcgttcgct ctctgtagag aacttttccc
     1081 gaacgccgtt attctcgttg acaactgcta cggcgaattt gtggaagcgc tggagccgac
     1141 agcctgcggt gcggatctga ttgccggctc gttgatcaag aaccccggcg gaggacttgc
     1201 gccgacgggc ggatatatcg ccgggaagaa ggaacttgtc gaacgcgcgg cggaacggct
     1261 gaccgttccc ggtatgggcg gggaagtagg ttcctacgcg ggcgactaca ggctgtttta
     1321 tcagggactg tttcttgcgc cgcatgtgac cgcgcagtgc gtcaaaagcg cggcgctgtt
     1381 tgcgcgcgta ttcgaaacgc tggaactttc tacgatgccc gcaagcgacg ccgttcgttc
     1441 cgacatcatt caggcggtgc ggtttgagga cgcggactcg ctggtgcgct tctgcggtgc
     1501 gattcaaagc tcggctccgg tggacagctt cgtttcccct gaaccctggg acatgcctgg
     1561 atacgacgat cctgtcgtga tggcggcggg agctttcgtg cagggcgcaa cgaccgaact
     1621 ctccgcggac gggccgatac gcagccccta cacggcgtat cttcagggcg cgctgaccta
     1681 tgcgcatgcg cggattgcgg caatgctcgc ctgcgacgca ctgcaaaaag aataaactcc
     1741 cgcgctgaaa acgcaggagt tatacaatca aaattaggtt ccgaactggc tagatcgtgc
     1801 gcagaaggcc aatgaccttg ccggagattt ccaaatcatt gaacgcaaca tagatcggtt
     1861 caaacaggtc gttttccggc tggagacgga cgcggtcttt ttcgcggaag aaccgtttga
     1921 ccgtggcgct ttctcccgcg acgcgagcca ccacgatgtc gccgttggtg caggaacagt
     1981 tgtggttgac aacgaggatg tcgccgtcac gaatgcctgc gtcccgcatg ctctcgcctt
     2041 cgacacggag catgtagact tcttccggct ccttaccgtg catgagaacg tccggcaggg
     2101 gaaacgcatc ctcaatattt tccgtggcaa gcattggcat gccggcggca acgtggccaa
     2161 cgagcggaac ggcgtatttt ttctcctcac gttcggcacg gtcgggaatg ttttcggaat
     2221 ggatgaagat ggcgcgctgc ttggaagagt cgcgcgtaat catgcctttc ttctcaagat
     2281 tctgaagatg cgcatggacg gttgccgtgg acttaagccc gaccgccgcg cagatttcac
     2341 gaaccgacgg gggataggaa tgctcgtcgg cataggaaat catataatcc agaatgacct
     2401 gctgcggatc ggaaagtttt ttcataactt aacttgcctc cgtttcgtgc ttcagcgaaa
     2461 atattgtgcc ggttcgattc cttcggcttg actttttaga aattatacca tatactctca
     2521 tcgattgcaa acatatgttc gaaagattgg tgacgttttg tgaaacgatg tgttctgcac
     2581 gcggaaaaga cctgcacaaa ctgcggagaa tgcgacgaca ggtgcgagct tgacccgcag
     2641 aagatttgcg acaactgctt tcggtgcctt gaaaccgatc aaaaatcgtt tttggagatt
     2701 ccggtttcag gcgtctattt tgaggatgat tttacgccgg aacccgtttt gcccgcggaa
     2761 ctcgaactcg acgaggatga ggttcccgtg ctggattctg acgattcctg gagggaagag
     2821 ttccatcttg gcgtttcaac gctgaacggc gcaacggctt cccgcgcaaa acggcggcat
     2881 attcggcgct acttcgtata gccgagtcac ggagaagacg atacagtcct gttcaatcgt
     2941 cccgcagctt aatctttttt gcggcgattc gcttatgctc ctcggaagtg gcggcatagt
     3001 gcttgcgggt ggtgttgacg tccttatggc ccagcacgtc cgcaacgagg tagatatcct
     3061 ccgtttcgtg ataaagcatt gttccgtagg tgctcctgag cttatgcggc gtgatcttct
     3121 tgagcggagc tgcaaggtgt gcgtatttct taacgaggtt ctcaaccgcg cgcgctgtga
     3181 tgcggcgcat ctggatggaa agaaacattg cctgctcatg cccgggcagg ggagtctgtg
     3241 ccgaacgcca ggaaagatag tcgctgagcg ctttgtgcac ttcctcgcca aacgacagga
     3301 tatcctcgct gccgcctttg cgggtgacca cgaacgcgtc attttccaaa tcgacgtcgc
     3361 ctagatcgat acccacgagc tcgctgatgc ggatgccggt gccgagaaac agcgttaaga
     3421 tcgccagatc gcgaatctta gtcgcgctgt gatattgttt ttgccggtcg ctcagccctt
     3481 cgccgttttc ggtcaggtct agcagatccg caacctcgtt cggctccagc cggataatcg
     3541 ccttttcgtg cagcttgggg atatccacca gcgcggcaac attgttgggc agcatctcct
     3601 gcttatgaag atacttgaag aacgagcgga tagagctgag ctttcgcgcc tttgcctgct
     3661 cctgattgac cagttcgcgc tcgccttttt gataaaacga cacttcgtta agaaacagtt
     3721 cgatatgata cgccttgatc ttttccaaat cgcttgccgt gagggagggg atgggttttc
     3781 ccgggaacaa atcttcctgc gcggaagcga actgaaagaa accgcgcaga tcgattgcgt
     3841 acgcgtatct tgttaaaacg gatgtctgac tctcgattgc tcgaaagaac gagccgcaga
     3901 ccaatggaag ctccggtaac agcgctcgaa ttttcattgt atagtctttg aacttttccg
     3961 cctggaacgt attagccatg ggggatcgct ccttaaaaaa ctctcgaacg gcggggaatc
     4021 actcgataga tacatttttt tactctattt actctatcac aactattcgt taaagacaag
     4081 tttaacgaat agatagagca aaaaatcaac aattacgatg gattttgatc ggtttggccg
     4141 ttttgagact gcggcgcggt gttttgcttg attgcctgcc tggcaagcgc gtcacagcgg
     4201 ttattgaatt cgttgtcgga atgtccttta actttgacga acgtaaactg gtgttctttt
     4261 aagatatcaa gcagcgcgcg ccagagatcc tgattctcaa cgggccgctt tgaaacagtt
     4321 ctccagccgt tggccgacca gttctccagc cagccgttat gaaatgcgtt gcagacgtaa
     4381 gcgctgtcgg tatgtacggc aatctcgcaa ggtatcttca acgcgcggat tgcctcgata
     4441 accgccttta gttccatgcg gttattggtc gtatccggct cgaatccgga aagctctcgg
     4501 gtttgatctt tgtacagcaa aatcgcaccc cagccgccag gacctggatt gccggagcat
     4561 gcgccgtccg tatagattgt caccttcttt ttcatgcttc cccgttatta tatataatat
     4621 ataatatgta gattctactg tttgctcatt ttttcggact tttcacggca ggcgtccacg
     4681 gcggcgatga ccgtaccgcg gagacctttt tgctcgagca cggcgacagc ttcaattgtc
     4741 gtcccgcccg gcgagcaaac gcggtccttg agctcacccg gatgcacgcc
//
LOCUS       DAOMGY010000028         4767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_18255_length_4767_cov_3.446095, whole genome shotgun sequence.
ACCESSION   DAOMGY010000028 DAOMGY010000000
VERSION     DAOMGY010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18255_length_4767_cov_3.446095"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLR57_00870"
     CDS             complement(<1..338)
                     /locus_tag="PLR57_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40035.1"
                     /translation="MKQQKTGPSAIERKLAGAPAPQAVSKNNGGDEEFNALVNEVSTL
                     IAENKLPEGFDFQMAASDPELVELMREYGAAAGIRIFAAEQRAEEAETSAMQRVSAEV
                     RQRSALPRSVR"
     gene            complement(363..1979)
                     /locus_tag="PLR57_00875"
     CDS             complement(363..1979)
                     /locus_tag="PLR57_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40036.1"
                     /translation="MKATKQTDEKRILCEKAYGLFREFRGAYVQEWQRLENCERMYRG
                     DHWHDVPVTDRNEPRPVTPILQSTIENIKSELMDRVPEAVILPESPADADVARVIEAI
                     IRQNHDADGYSKEYRKLVHDLLVGGYCVQEVGYDSTLNSGLGGAFIRHVDARSILFDP
                     LATDMQESRAVFKISLKTREYLLAHFPKAAPFFTADAFGTPDAVQDEYLRGERSDAML
                     FVEYWWRDYDAETDRTSVHMAQIAGNVVLTDSRDVKPEGYYEHGFYPFIITPLFVRKG
                     SCLGLGFVDMFETQQRYADKLDQIVLKNALMASHNKLLVTEASGFDVDDLRDWSKEVH
                     RGESLGGVTWFSTPPLPAYIIGYIESIREGIKQESGANDFSRGMPDGGVTAATAISAL
                     QEMSNKRARMIARSLHESFRDAVRFEIEVEREFNFFTRRVNVTVDGKVQERSFESVML
                     MRRAPGSVYLPLEFCISVKAQQETRYSAMSQNELILQMLQNGIVRPEQAVELMVFEGK
                     DQLLKQLRERREQEEAANREKNEGQPGLPA"
     gene            complement(2005..3360)
                     /locus_tag="PLR57_00880"
     CDS             complement(2005..3360)
                     /locus_tag="PLR57_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HQQ40037.1"
                     /translation="MAGANDTEILLKGYPNPRQKEFFCSRARHTAYGGARGGGKSWAM
                     RRKFVLLALRYSNLQLLLLRRTLPELNENHARPLQTELSGFVNYNQTHREFTFPNGSR
                     IKLGYCDNEKDVYQYQGQEYDVIGMEEATHFTESQMQFITTCNRSVRSDFSPRMYYTC
                     NPGGVGHAWVKRLFIDRRYRLSEQAEDYVFIPARVTDNPVLMKTPSYLKTLENLPEPL
                     KRAHLNGDWDVLAGQYFGEFSRERHVLEPFTVPKSWRKFRAMDWGYNDPCCVLWFAVS
                     PGGRVYVYREYYEREVLSSETAKRIRSLTGEEKIAYTAASPDAWQCRGMNASGELPGM
                     SIAEVFAHGGVPLLRADNTRVPGWQRVREYLADGDDGQPRLGIFRSCQNLIRTLPMLT
                     FDENFVEDVSGACEDHAPEALRYGLMSRPVSFVEKNQPKARAYDPFLAAEPSADGFTR
                     L"
     gene            complement(3344..3742)
                     /locus_tag="PLR57_00885"
     CDS             complement(3344..3742)
                     /locus_tag="PLR57_00885"
                     /inference="COORDINATES: protein motif:HMM:NF027987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HQQ40038.1"
                     /translation="MKPAGEPTLESKIKAYFAACDATAERTELKNGSVEVRQTPYTLA
                     GLSEHLGIPSGEILAAARREDGGAEGELFAQAARRIGRYLVERALTQELQYSVAAKLL
                     DELGIGEKLPPDEREKRIVIVLEDPNGWSE"
     gene            4061..4579
                     /locus_tag="PLR57_00890"
     CDS             4061..4579
                     /locus_tag="PLR57_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40039.1"
                     /translation="MKKAFSILVALVLIFLFASCKQPAAEETTQLPNPIVDVESSADF
                     EPLGVSITAPEGAENVAYSIIAKVTAQINFTLDGRAYTYRAAKTTDDISGVYVTFDET
                     QETFNLDAADFSVSVLVRTIGGGTDGALATWSLDGVAYTLYSADASTIDSMSDIALLC
                     AYADLPFGACIG"
     gene            complement(4663..>4767)
                     /locus_tag="PLR57_00895"
     CDS             complement(4663..>4767)
                     /locus_tag="PLR57_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003363046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQQ40040.1"
                     /translation="AISEGSSIYVIDADKCIDCGNCADVCPVAAPKAE"
BASE COUNT          860 a         1484 c         1343 g         1080 t
ORIGIN      
        1 cgcacgcttc tgggcagggc gctgcgctgg cgcacttcgg cgctcacgcg ctgcatggcg
       61 ctggtttccg cttcctctgc gcgctgttca gcggcaaaga tgcgaatccc cgccgccgcg
      121 ccgtattcgc gcatcagctc gacgagttcc ggatcggaag ccgccatctg gaagtcgaac
      181 ccctcgggca gtttgttttc ggcaatcagc gtggatacct cgtttacgag cgcgttgaac
      241 tcctcatcgc cgccgttgtt ttttgaaacg gcctgcggag cgggcgcgcc tgcgagcttc
      301 ctttcgattg cgcttgggcc tgttttctgc tgtttcatgt gttcctcctc atatgatgcc
      361 ggttatgcgg gcagccccgg ctggccttcg tttttttcgc ggttcgccgc ttcttcctgc
      421 tcgcggcgtt cgcggagctg tttgagcagc tgatccttgc cttcgaatac catgagttcc
      481 acggcctgtt cggggcgcac gatgccgttc tgcagcatct gcagaatgag ctcgttctgg
      541 ctcatggcgg aatagcgcgt ttcctgctgc gctttgacgg agatgcagaa ctcgagcggc
      601 agatatacgc tgcccggcgc tctgcgcatg agcatcacgc tttcaaacga gcgctcctgc
      661 acctttccgt ccacggtgac gttgaccctg cgggtaaaga agttgaactc gcgctcgact
      721 tcgatttcaa aacgcaccgc gtcccgaaac gactcgtgga gcgaacgcgc tatcatccga
      781 gcgcgcttgt tgctcatctc ctgcagagcg gaaatcgcgg tggccgccgt cacgccgccg
      841 tccggcatac cccggctgaa atcgttcgcc ccgctttcct gcttaatgcc ttcgcggatg
      901 ctctcgatgt agcctatgat gtaggccggc agcggcggcg tggaaaacca cgtaaccccg
      961 ccaaggctct caccgcggtg cacctctttc gaccagtctc tgaggtcgtc cacatcgaaa
     1021 ccgcttgcct cggtgacgag cagcttgttg tgggaagcca tcagggcgtt tttcagcacg
     1081 atctggtcga gcttgtcggc ataccgctgc tgggtttcga acatatccac gaatcccagc
     1141 cccaggcagg aacctttgcg cacgaacagc ggcgtaatga tgaacgggta aaagccatgc
     1201 tcgtaatagc cttcgggttt tacgtccctg ctgtcggtga gcaccacgtt gcccgcaatc
     1261 tgcgccatgt gcacgctggt gcggtcggtt tccgcgtcgt agtcgcgcca ccagtactcc
     1321 acaaagagca tcgcgtcgct gcgctcgccg cgaaggtact cgtcctgcac cgcgtcgggc
     1381 gtgccgaacg cgtccgccgt gaaaaacggc gcggccttgg gaaagtgcgc cagaaggtat
     1441 tcccgcgtct tgagcgaaat tttgaacacc gcccggctct cctgcatgtc cgtagccagc
     1501 gggtcaaaaa ggatgctgcg cgcgtccaca tgccggataa acgccccgcc aagcccgctg
     1561 ttgagcgtgc tgtcgtaacc gacttcctgc acgcagtacc cgccgaccag caggtcgtgc
     1621 acgagcttgc ggtattcctt gctgtagccg tccgcgtcgt ggttctgtcg gataatcgcc
     1681 tcgatgaccc gcgcaacgtc cgcatccgcc ggactttccg gcaggataac cgcctccggc
     1741 acgcggtcca tgagttcgga cttgatgttt tcaatcgtgc tctgcagaat cggcgtgacc
     1801 ggacgcggct cgttgcggtc ggtcacgggc acgtcgtgcc agtggtcgcc gcggtacatc
     1861 cgctcgcagt tttccagccg ctgccattcc tgcacgtacg cgccgcggaa ttcccgaaac
     1921 aggccgtaag ccttttcgca gaggatgcgc ttttcgtccg tttgtttggt tgctttcatt
     1981 tgttcctcct atgggaagtc ccgtttacag ccttgtaaat ccgtccgcgc tgggttcggc
     2041 ggctaaaaac gggtcgtaag cgcgcgcctt cggctgattc ttttctacaa agctgaccgg
     2101 cctcgacatc agcccgtagc ggagcgcttc cggcgcgtga tcctcgcaag cgccgctcac
     2161 gtcctcgacg aaattctcgt caaacgtcag catcggcagc gttcggatca ggttctggca
     2221 agagcggaag atgcccagtc tcggctggcc gtcgtcgccg tccgccagat attcgcggac
     2281 gcgctgccag cccggcaccc gcgtattgtc cgcgcggagc agcggcacgc ccccgtgggc
     2341 gaacacctcc gcaatgctca tgccgggcag ttccccgctt gcgttcatgc cgcggcattg
     2401 ccaggcgtcc ggcgaggcgg cggtgtatgc tatcttctct tcgcccgtta aagaacggat
     2461 gcgctttgcc gtctcgctgg acagcacctc gcgctcgtaa tactctcgat acacatacac
     2521 ccgcccgccg ggcgaaacgg caaaccacag cacgcagcag ggatcgttgt agccccagtc
     2581 catcgcgcgg aacttccgcc aggacttggg caccgtaaac ggctccagca cgtgccgctc
     2641 cctcgaaaac tcgccgaagt actgccccgc cagcacgtcc cagtccccgt tgaggtgggc
     2701 gcgcttcagc ggctccggca gattttcgag ggtcttgaga taggacggcg tcttcatcag
     2761 caccggatta tcggtgacgc gcgccgggat aaaaacgtaa tcttccgcct gttcgcttaa
     2821 tcggtacctg cggtcgatga agagccgctt gacccatgcg tgacctacgc cgccgggatt
     2881 gcatgtgtag tacatgcgcg gcgaaaagtc gcttcttacc gagcggttgc aggtcgtaat
     2941 gaactgcatc tggctctcgg taaaatgggt tgcttcctcc atgccgatta cgtcgtattc
     3001 ctggccctga tactgataga cgtccttctc gttgtcgcaa taccccagct tgatccggct
     3061 gccgttcgga aacgtaaatt cgcggtgcgt ctggttgtag ttcacaaacc cggacaattc
     3121 cgtctgcagc ggtcgagcat ggttttcgtt cagctccggc agcgtacgcc ggagcagcag
     3181 gagttggagg ttggaatacc ggagcgcgag caggacgaac tttcgccgca ttgcccagct
     3241 ttttccgccg ccgcgcgcgc cgccgtaagc ggtgtgccgg gcgcgggagc aaaagaactc
     3301 cttctgacgc ggatttggat acccctttag taagatttcc gtatcattcg ctccagccat
     3361 tcggatcctc cagcacgatt acgatgcgct tttcgcgctc gtccggcggc agtttttcgc
     3421 cgatgccgag ctcgtccagc agtttcgccg caacgctgta ctgcagctcc tgcgtcagcg
     3481 cgcgttctac gaggtatcgc ccgatgcggc gcgccgcctg ggcaaacagt tcgccctcgg
     3541 cgccgccgtc ctctcttctg gctgcggcaa gaatctcccc cgatgggatt ccgagatgct
     3601 cgctcaggcc cgcgagcgta tacggcgttt gacggacttc tacgcttccg ttcttcagtt
     3661 ccgtccgttc cgccgttgcg tcgcacgcgg caaagtacgc tttaattttg ctttcgagag
     3721 ttggttcacc ggccggtttc atcgttcgtg ctcctttctt tgcgacgacg gcggaacgtt
     3781 cgaggccgtg tcttttccgc cgtcacaggt atcgtaacgc acaaaaaaca cctaaattta
     3841 cgacaaaaat aattcgaata atgcaattct tacaattccg gccgtttctg cggggcgcag
     3901 atttactcag taaaatcctt aaacgcaaaa aagccgccgt tttcggcggt ttcggcttgc
     3961 ttttcagttg aaggaaaaaa tggtaaaata gagaaaattg aaatgaatgc atgcaaccaa
     4021 acacctttat aaacggtgtt actggcggag ggataaacac atgaaaaaag ctttttcgat
     4081 tctggtcgcg ctggtgctga tctttttgtt cgcttcctgc aagcagcccg ccgcggagga
     4141 aaccacgcag ctgcccaacc ccatcgtgga cgtggaaagc agcgcggatt tcgagccgct
     4201 tggcgtttcc attaccgcgc cggaaggggc ggagaacgtt gcctacagca tcattgcaaa
     4261 ggtcacggcg cagatcaact ttacgctcga tggacgcgcg tatacctacc gcgccgcaaa
     4321 gacgacggac gacatctccg gcgtatacgt aaccttcgac gaaacgcagg aaaccttcaa
     4381 tcttgacgca gccgatttct ccgtctccgt gctggttcgc accatcggcg gcggcacaga
     4441 cggcgcgctt gccacctgga gtctggacgg cgttgcctac acgctgtaca gcgcggacgc
     4501 ttccaccatc gacagcatga gcgacattgc gcttctctgc gcttacgcgg atctgccgtt
     4561 tggcgcctgc atcggctgaa gctggcgctg ttgtcccctt tcactttaaa tagcaaaaaa
     4621 agaaggacgg cgtatgccgt ccttcttttt ggtttgtaaa ggttactccg ctttcggtgc
     4681 ggctaccggg caaacgtccg cgcagttgcc gcagtcgatg cacttatcgg cgtcgattac
     4741 gtaaatgctg gagccttcgc tgatcgc
//
LOCUS       DAOMGY010000029         4677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_18753_length_4677_cov_2.782129, whole genome shotgun sequence.
ACCESSION   DAOMGY010000029 DAOMGY010000000
VERSION     DAOMGY010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18753_length_4677_cov_2.782129"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2201
                     /locus_tag="PLR57_00900"
     CDS             <1..2201
                     /locus_tag="PLR57_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQQ40041.1"
                     /translation="RDTLQAAKDALNAWNVYLPAHETSQTAQALDAAIQQAALDAATA
                     QAILDAYTVLSSWTATAAEKNAAKLLIDTYGLTKTQAKAALAKATADQTAAEAALTEL
                     LSQNSLASVQAAIDAETAARSALDTTKAALNAYAAANEAQQAVNEAEQRLSDANTQYG
                     VIDSLIGVRDAAITALSTAQSAMETAYQNWVDVRLAAGTDEDALVQSALADYLAAQAE
                     RDEKSATLDALNAELALLPTLSQAQDEIDSAEAALTLAEQNLADALAAQPLVTPDSFG
                     ADFAANDATGVELPTIRTSGNLTIHTGGNAGTDTEPLMIDAAGTVLLNATGTVSLAGA
                     HSLLLGGVTGSSVTIAANGSIMDVSPAGAQMITADKAVLYAIDGIIGGSFAKTNINYF
                     SGFADAIYIQNLGALELGPVSAYGAGIFLITGGAITQSAGGFLQAPNIELTAGGAIGT
                     PGAPVIVNTQSLTANGGNLYFSSIGHLNRVDLTGGSVVLSIGGNLTGGIIRAKNLTLI
                     AFGSIGTAQNPLRLVVPGALIITSLYGEIFYTNQLIRSTFLVGLLLGGDVPMMLLIRF
                     GQTIQGGLVVMNILMVRLDDLSGFGDVLAGAGIRSLDMALIEGANGSSSVLAKALLAA
                     FPKVKIAYSALLWIAQMRPLIAEDDWSAMLSDLEAIYRATTEQALTDAVEAFTEHWQA
                     AYPEVTARLNQEAADSLAMLEAFDLMHRSELDLTMLLNALSEVLAQANLS"
     gene            2364..3605
                     /locus_tag="PLR57_00905"
     CDS             2364..3605
                     /locus_tag="PLR57_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ40042.1"
                     /translation="MEENKPQKQTGWIRSWWTRGVAAFLAVAMTCTIVLANAVRIQLG
                     ADGESASEQALSETTDYINKNPLARAGDVITRFFTNPQTLEQYYQNASVLIGAGQYEQ
                     ALGLIKKCITMADTSDAALLDELWLKCGCLETLTKQYDAALESFTHISEGTYTAELLL
                     IKAQIFDEQGETEQTISALESYLLLHPEDNDTRNTLAGILLEQERYDAAILQYDAILA
                     NGGANGDGYMQRASAKLMAGDYEGAVIDFLSAKDAGYADPSACYAQCALASYLKEDYP
                     SVVSYGEQAIEIGSQNFTYETLYYYMGLAQLNLEAYDQAAELFTKSIDMGIGMVEAHY
                     YRGACYMVNGNMQGAVEDFTIVIDQNVTMLLSNSYFNRGVCYADLQVYAAAKSDLQKV
                     LELEQEGDLYESAKTMLDLLN"
     gene            3905..4150
                     /locus_tag="PLR57_00910"
     CDS             3905..4150
                     /locus_tag="PLR57_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40043.1"
                     /translation="METQTVKLTVPKEKIIHIAILTVLACAIILFGAGLGAYCALNQV
                     SYSVLTARIPGYAFGLIIAALGIRYLFSIYKRVKKEF"
     gene            complement(4291..4674)
                     /locus_tag="PLR57_00915"
     CDS             complement(4291..4674)
                     /locus_tag="PLR57_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQQ40044.1"
                     /translation="MFSILAALLLLAACAPKTPVAEYHKITAEQAKERLDSGDELILL
                     DVRTQDEFNAGHIAGAMLLPNETITETEQPDILPDLNAEILIYCRSGNRSAQAAKKLV
                     AQGYTNVYDFGGIIDWPYETVTGAN"
BASE COUNT         1074 a         1262 c         1381 g          960 t
ORIGIN      
        1 caagagacac ccttcaggcg gcaaaagacg cgctgaacgc atggaacgtc tatctgcccg
       61 cgcacgagac ttctcagacg gcgcaggcgc tcgacgccgc gattcagcag gctgctctcg
      121 acgcggccac ggcgcaggcg atactcgacg cttacaccgt gctctcctcc tggacggcga
      181 cagccgccga aaagaacgcg gcaaaacttt tgattgatac ctacggactt acaaagacgc
      241 aggcaaaagc cgcgctggca aaagcgaccg ccgatcagac ggcggctgaa gccgcgctga
      301 ccgaactgct gagtcaaaac agccttgcca gcgttcaggc ggcaattgac gcggaaaccg
      361 ccgcgcgcag tgcgctcgat acgacaaaag cagcgctgaa tgcgtacgct gccgcaaacg
      421 aagcgcaaca agctgtaaac gaagccgaac agcggctcag cgacgcaaac acgcagtacg
      481 gcgtaatcga ctcgctgatc ggcgtgcgtg acgctgcgat caccgctttg tccacggcgc
      541 agtcggctat ggaaaccgct taccagaact gggtagacgt gcgcctcgcg gccggaacgg
      601 atgaggatgc tttggtgcag tccgcgctgg cggattacct tgcggcgcaa gcggagcgag
      661 acgaaaagtc cgccactctc gacgcgctga acgcagagct tgctctgctg ccgacgcttt
      721 cacaagcgca ggacgaaatt gacagcgctg aggcggctct tacgctcgcc gaacagaacc
      781 tcgctgatgc gcttgctgct cagccgctcg tcacgccgga cagctttgga gcggactttg
      841 cggcaaacga cgcaaccggc gttgaactgc cgaccatacg cacgagcggc aatctgacga
      901 ttcatacagg cggcaacgca ggaacggata ccgaaccgct catgatcgac gcggcgggaa
      961 ctgtgctgtt gaacgcaaca gggacggttt cgctggcagg cgcgcattcg ctgctgcttg
     1021 gcggggtcac cggaagcagc gtaacgatag ccgcaaacgg ctccatcatg gacgtttctc
     1081 cggctggcgc gcagatgatt accgcggaca aagcggttct ctacgccatt gacggaatca
     1141 tcggcggcag ctttgcaaag acgaacatca actacttcag cggctttgcg gacgcgattt
     1201 atattcagaa cctcggcgcg ctggagcttg gccccgtttc ggcttatggc gcgggcatct
     1261 tcctgatcac cggcggcgcg ataacacaga gcgcgggcgg tttcctgcag gcgccgaaca
     1321 ttgaactgac tgccggtggc gctatcggaa cgccgggcgc gccggtaatc gtcaacacgc
     1381 agtcgctgac cgcaaacggc ggaaatctgt atttcagcag tatcgggcat ctgaacagag
     1441 tcgatctcac cggcggaagc gtcgtgctct caattggcgg gaatctcacg ggcggtatca
     1501 tccgtgcaaa gaacctgacc ctgatcgcgt ttggcagcat cggtacggcg cagaatccgc
     1561 tgcggcttgt cgtgccgggc gcgctgatca ttacgtcgct gtacggagaa atcttctaca
     1621 ccaatcagct tatccgctcg acgttccttg tcggcctgct tctcggcggg gatgttccga
     1681 tgatgctcct gatccgcttc gggcagacga ttcagggcgg tctggttgtg atgaacattc
     1741 tgatggtaag gctggacgat ctctccggct ttggcgatgt gcttgccggc gcgggcattc
     1801 gctcgctcga catggcgctg atcgaaggcg cgaacggttc ctcctcggtg ctcgcaaaag
     1861 cgttgctggc ggcgttcccg aaggtgaaga tcgcctattc tgcactgctc tggattgcgc
     1921 agatgagacc tctgatcgcg gaggacgact ggagcgcgat gctgagcgat ctggaggcaa
     1981 tctaccgcgc gacgacagaa caagcgctca cggacgctgt ggaagccttt accgaacact
     2041 ggcaggcggc gtatccggaa gttaccgctc gactgaatca ggaagcggct gattctttgg
     2101 ctatgctgga agcgtttgat ctgatgcacc ggtcggaact ggatctgacg atgctgctca
     2161 acgccctatc cgaagtgctg gcgcaagcga atctttctta acggatagac aggacgggga
     2221 agcggctgaa aaagcgccgc gcgttctgct ggatcagttt tcgcaaccgg ctttcatgca
     2281 tgtgggagcc ggattgcttt atgccgggcg ctttggttgc gcacgcatga gccgcgtggt
     2341 atcattcact taacgaggac gtcatggaag aaaataaacc ccaaaaacag acaggctgga
     2401 tccggtcgtg gtggacgcga ggcgttgccg cgtttcttgc ggtggcgatg acgtgtacca
     2461 tcgttctagc aaacgcggtg cggattcaac tcggcgcgga cggcgaatcg gcttccgagc
     2521 aggcgctcag cgaaacgacg gactatatca acaaaaaccc gctggcgcgc gcgggggatg
     2581 tgattacacg gtttttcacc aaccctcaga cgctggagca gtattatcag aatgcgtccg
     2641 tgctcattgg cgcgggacaa tacgagcagg cgctcgggct gatcaaaaag tgcatcacca
     2701 tggcggatac gagcgacgcc gcgcttctgg acgagctctg gctcaagtgc ggctgtctgg
     2761 aaacgctgac gaagcagtac gacgcggcgc tggaaagctt tacgcacatt tcggagggca
     2821 cgtataccgc agaactgctt ttgataaagg cgcagatatt cgacgagcaa ggcgaaacgg
     2881 aacagacgat ttccgcgttg gaatcgtatt tgcttttgca ccccgaggat aacgatacgc
     2941 gcaacaccct cgcgggaatc ctgttggagc aggagcgata cgacgcggct attttgcagt
     3001 acgacgcaat tttggcaaac ggcggagcaa acggagacgg ctatatgcag cgcgcttccg
     3061 ccaagctgat ggcgggggat tacgagggcg cggtgatcga ctttttgagc gcaaaagacg
     3121 cgggttacgc cgatcccagc gcatgttatg cgcagtgcgc gctggcgagc tacctgaagg
     3181 aagattatcc gagcgttgtg agctacggcg agcaggcgat cgaaatcggg tcgcaaaact
     3241 ttacgtacga aacgctgtat tactacatgg gccttgcgca gctgaatctg gaagcgtacg
     3301 atcaggcggc ggaactgttc accaaatcga tcgatatggg cattggcatg gtggaagcgc
     3361 actattaccg cggcgcgtgc tacatggtca acggaaacat gcagggcgcg gtagaagact
     3421 ttacgattgt catcgaccag aacgttacaa tgcttctttc caacagctat tttaaccgcg
     3481 gggtctgcta tgccgatttg caggtctatg ccgcggcaaa gagcgatctg cagaaagttc
     3541 tggaacttga gcaggaaggc gacctttacg agtcggcaaa aaccatgctg gacttgctga
     3601 attagcgggt tggtgaatat acgtcagaac ttacccccat accgaaacga caagaaatcc
     3661 atcttttcca caaaagcagc aggttctgca atttgtgtca gcgtctacaa aatgtcccac
     3721 ctattgacaa acatccaccg atgtagttta atctcaaaat agaaagcaaa ggcgctgcag
     3781 gattcttgcg gcgctttcgc ttttttggct tttcggcagg cacagcgaat cagaacaaca
     3841 ggttcgattg agggcgatga aacatcgttc aactgatagt attcgatgag aaggagggca
     3901 aacgatggag acgcaaacgg tgaagttaac ggtaccaaag gagaagatca tacacatcgc
     3961 aatccttacc gtattggctt gcgcaatcat cctgttcggg gcggggctcg gcgcatactg
     4021 cgcgctgaat caggtatcgt actcagtgct gaccgcgcgg atacccggct acgcgttcgg
     4081 tctgatcatt gccgcccttg gcatacgata cctgttctca atttacaaga gggttaaaaa
     4141 ggaattctag attacgccga acttgtcatg actcgaattt gcagacttgc tttcaagtct
     4201 agctataaat atcaagataa tacagaaaaa agcacagaca aaaaacgaac cccaaaggtt
     4261 cgttttttgt ttcttatgtc ggttcgaaga ttagttcgct ccggttaccg tttcatacgg
     4321 ccagtcgatg attccgccga agtcgtaaac gttggtatac ccttgcgcca cgagtttttt
     4381 cgccgcctgc gcgctgcggt tgccggagcg gcagtagatc agaatctccg cgttgaggtc
     4441 gggcagaatg tccggctgct cggtttcggt gattgtttcg ttgggcagca gcattgcccc
     4501 cgcaatatgc cctgcgttga attcatcctg cgtgcgaaca tccagcagga tgagctcgtc
     4561 tccgctgtcc agacgttctt ttgcctgctc ggcggtgatt ttatggtact cggcaacagg
     4621 cgttttgggc gcgcaggccg caagcagcaa cagcgctgca agaatggaga agatcgg
//
LOCUS       DAOMGY010000030         4653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_18897_length_4653_cov_3.015659, whole genome shotgun sequence.
ACCESSION   DAOMGY010000030 DAOMGY010000000
VERSION     DAOMGY010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18897_length_4653_cov_3.015659"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="PLR57_00920"
     CDS             <1..1132
                     /locus_tag="PLR57_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40045.1"
                     /translation="YVELLTRVFGAKGILIAAPVLNVLLLVFSYASILKTTGRLFRDN
                     RVTLLALLLLCVSFQPLLLCTSIDAGVLAAALTVWAMGRIVLYMQYGKKANLIWGAVF
                     AMLAAALSSVGRAAAVALALMIGLYVLKHKKWFPAVTAFVLLAVSMFVPVGMRLVYQA
                     RLETDFGAGLPAVVWKAAESENGASVSHEGARAYVDGLREEHDLDFSAYAAQAKEDLA
                     ESAEVELAPAARLFKRLNGEWNEPTFASVWSSGAFEPFGERSNYAERIYEASREGEGL
                     NGFFAQSLTLLYLGAFLASMVFIKKRSTEQMVLPLIVLTGVLSLAGYGAETPQTVRLL
                     PLLCPLAAYGLLSFGLDLTVYLTRPETSWEEAKSGRRRRNVK"
     gene            1229..2656
                     /locus_tag="PLR57_00925"
     CDS             1229..2656
                     /locus_tag="PLR57_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQQ40046.1"
                     /translation="MRKQLAALFDRSFWRVVLPLALPIALQNLLMTSFRLIDTLMIGR
                     LGDVSIAAVGLAGYASFLVELISFGMASGSAVFVAQYHGAENKEGILRTFGMMLLFMV
                     PVGLLFTLGVGIFPQEVMRLFTEDAALISEGAKYLQFACISYFSLTLNMAFSTLLRCT
                     EQVRIPMITSGICAALNAVLNYIFIFGAFGIPAMGVAGAGLATAISSLLNPLLMFVLS
                     AIKKTVIIAPFKKIFAVRGFFKTFWSRALPVLLNELFWSLSVVGINMVFGRMGTDNYA
                     ALTVERTIEGLVFVFFVGICNACNILVGKSVGAGRIEEGKAYAKRFLLFTPLLGLVTG
                     GIIALLRVPLIGLFDLSDTASHTARMLLLIFSLDAVVRYIPYIEVVGIFRGGGETRFG
                     LLSDVVSQYVFILPAVILCAFVWKLPFLTTYIVMLAADDISKLALTIPYYKSMRWVKP
                     IESAPAPMPDSQEILLQNGDESGIL"
     gene            2776..4131
                     /locus_tag="PLR57_00930"
     CDS             2776..4131
                     /locus_tag="PLR57_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HQQ40047.1"
                     /translation="MRKMRLGIKPINVLPLGFIAVILTGTMILMLPISSKDGSSLSLV
                     NALFTATSASCVTGLVVVDTGTYFSLFGQLVILLLIQLGGLGIMTMSMILFGLTGRKI
                     SLHDRLSMAEGLGENRLQGVVRLARGALLVTGIFELVGAILLSIRFIPQYGVVKGIWF
                     SIFHSISSFCNAGFDLIGGYRSFTEYKADPFFLMVVMMLIVGGGLGFGVLLNIWRQRD
                     FRRMRLHSKMVLTGTAVLILFGMLSFLIIEYDNPDTIGSMPFFQKLLNSLFQSVTLRT
                     AGFNTIDEFSLRDASKGVSVVLMLIGAGPAGTAGGLKITTIFTLILAVRSYLRGRFDT
                     VVFGRTISLDQVRRALTIFFFGLVFVMGMTIALSVVEQHKAAGALGIMNQLFETTSAF
                     CTVGVSSGVTQESGIATRIILILLMYAGRVGLLTVAMSLIEGTTKEPVLHYPQEDIQI
                     G"
     gene            4203..>4653
                     /locus_tag="PLR57_00935"
     CDS             4203..>4653
                     /locus_tag="PLR57_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HQQ40048.1"
                     /translation="MSKSFIIIGLGRFGTSTARMLTTLGHEVLVVDKRADRVNGIKDS
                     VMHAVQADATDERALSQLGVRNFDTVVICIGDDIRASILTTVLCREMGAKKIVAKAQD
                     DLHQKLLIKTGADRVVQPEHDGGIRLARSLVAEGVLDSLDLSDEYCIN"
BASE COUNT          834 a         1257 c         1384 g         1178 t
ORIGIN      
        1 ctatgtggag cttttgaccc gcgtgttcgg cgcaaaaggc atcctcatcg cggcgcctgt
       61 gctgaacgta ctcttgctgg tattttccta tgcgtcgatc ctcaaaacca caggacggct
      121 gtttcgcgat aatcgagtga cgcttcttgc gctgctgctc ctctgcgtca gctttcagcc
      181 gctgctgctt tgcacgtcta tcgatgcggg ggtgctcgcc gccgcgctga ccgtttgggc
      241 aatggggcgc atcgtgctgt acatgcagta cggcaaaaaa gcgaacctca tctggggggc
      301 ggtttttgcc atgcttgccg ccgcgctcag ctccgttggc cgcgcggccg ccgtcgcgct
      361 ggcgttgatg atcgggctgt atgtgctcaa gcataaaaag tggttccctg cggtcaccgc
      421 gttcgtcctg ctggcggttt cgatgttcgt ccccgtcggg atgcggctcg tgtatcaggc
      481 acggctggaa accgactttg gtgcgggact gcccgccgtg gtctggaaag cggcggaaag
      541 cgaaaacggc gcttccgtga gccacgaagg cgcgcgcgct tatgtggacg ggctccgcga
      601 ggagcatgat ctggattttt ccgcgtatgc cgcgcaggcg aaagaagatt tggcggaatc
      661 tgcggaagta gagctcgcgc ccgctgcgcg cctgttcaag cgcctgaatg gtgaatggaa
      721 tgagccaacg tttgcttccg tctggtcgag cggcgcgttt gagccgttcg gcgagcgttc
      781 gaactacgcc gaacgcatct atgaagcgag ccgggaaggc gaagggctga acgggttctt
      841 tgcgcagtcg ctgacgctcc tgtatctggg cgcgttcctt gcttccatgg tgttcatcaa
      901 aaagcgcagc acggagcaga tggttctgcc gctgatcgtg ttaaccggcg tactgagcct
      961 tgccggttac ggcgcggaaa cgccgcagac ggttcggctt ttgccgctgc tctgcccgct
     1021 cgcggcgtac gggctgctct cgttcgggct ggaccttacg gtgtatttaa ccaggccgga
     1081 aaccagctgg gaagaagcga aaagcggacg aagacgcagg aatgtgaaat aatcgaccaa
     1141 acgcgtatac ggcttgaaca cgcgtgcgct tgctggtata ctttgtgtct gttgcccgca
     1201 aaaggcggaa aagactgggg ggattgcaat gagaaaacaa cttgcggcat tgtttgaccg
     1261 ctcgttctgg cgagtggtgc tgccgcttgc gctccccatc gccctccaaa acctgctgat
     1321 gacctctttc cgcctgatcg acacgctgat gatcggccgt ctgggcgacg tttctattgc
     1381 ggcggtcggc cttgcggggt acgcttcgtt tctcgtggag ctgatttcgt ttggtatggc
     1441 gagcggcagc gcggtattcg ttgcccagta tcacggcgcg gaaaacaagg aaggcattct
     1501 ccgcacgttc ggcatgatgc tcctgttcat ggttccggtg gggcttttgt ttactcttgg
     1561 cgttggcatt tttccgcagg aggttatgcg cctctttacc gaggacgcgg cgctgatttc
     1621 ggaaggcgca aagtacctgc agtttgcctg catctcctat ttttcgctga cgctgaacat
     1681 ggcgttttcc accctgctgc gctgcaccga gcaggtgcgc ataccaatga taaccagcgg
     1741 catctgcgcg gcgctcaacg cggtgctcaa ctatatcttc atcttcggcg cgttcggcat
     1801 tcccgctatg ggcgttgctg gcgcggggct ggccacggcg atctcctcgc tgctcaaccc
     1861 cttgctgatg tttgttcttt cggcgatcaa gaagaccgtc atcattgctc cgtttaagaa
     1921 aatcttcgcc gtgcgggggt ttttcaaaac gttctggtcg cgcgcgctgc cggtgctgct
     1981 caacgagctg ttctggagtc tctccgtcgt cggcatcaac atggttttcg ggcgtatggg
     2041 aacggataat tacgcggcgc tcaccgttga gcgcaccatc gaaggcctgg tattcgtgtt
     2101 cttcgttggc atctgcaacg cctgcaacat tctcgtgggc aagtccgtgg gcgcgggcag
     2161 aatcgaagaa ggcaaggcgt acgcaaaacg gtttctgctc tttacgccgc tgctcggttt
     2221 ggtgactggc gggatcatcg cgcttttacg cgtgcctttg atcgggctgt tcgatctttc
     2281 cgacacggct tcccataccg cgagaatgct gctgctgatc ttctcactgg atgctgtcgt
     2341 gcggtatatt ccttatatcg aggtggtcgg cattttccgc gggggcgggg aaacgcgctt
     2401 tgggctattg tcggacgtcg tttcgcagta cgtgttcatc ctgccggccg tcatcctctg
     2461 cgcttttgtt tggaagctgc cgttccttac gacctatatc gtaatgcttg ccgcggacga
     2521 tatttccaaa ctcgctttaa cgattccgta ttacaaaagt atgcgctggg tcaagccgat
     2581 tgaaagcgcg cctgcgccta tgcccgactc tcaggagatt cttttgcaaa acggagacga
     2641 atcgggtata ctatagccgt tattttgttg ggggacgccc gattgggcga acccggagtt
     2701 tgcttttgac cgaatcaggt aaaccggcgc gatgaatgaa atagtttgtg ccggataccg
     2761 ggagaagtac gacttatgcg aaaaatgcgt ctggggataa agccgatcaa cgtgctgccg
     2821 cttggcttca tcgctgtaat attaaccggc accatgatct taatgctccc tatttccagc
     2881 aaggacggct cctcgctttc cttagtgaac gcgttgttta ccgcgaccag cgcttcgtgc
     2941 gttacggggc tcgtcgtcgt agataccggc acgtattttt cgctttttgg ccagcttgta
     3001 atccttctgc tcatccagct cggcggtctt ggcattatga ccatgtccat gatcctgttt
     3061 ggcttgaccg gacggaagat ttctctgcac gaccgcctta gtatggctga aggccttggc
     3121 gaaaaccgtc tgcagggcgt tgtccgcctt gcgcggggcg cgctgctcgt aaccggcatt
     3181 tttgaactgg tgggtgcgat tctgctttcc atccggttta ttccgcagta tggcgttgtc
     3241 aaaggaatct ggttttccat tttccacagc atttcatcgt tctgcaacgc agggttcgac
     3301 ttaatcggcg ggtatcgttc gtttaccgag tacaaggcgg atcccttctt cttaatggtg
     3361 gtcatgatgc tgatcgttgg cggcggcctg ggatttggcg tgctgctcaa catctggcgg
     3421 cagcgggatt tccgccgcat gcgcctgcac agcaaaatgg tgctgaccgg tacggccgtt
     3481 ttgatcctgt ttggcatgct gagctttctg atcatcgagt atgacaatcc ggatacgatc
     3541 ggctcgatgc cgttctttca gaaactcctc aactcgctgt ttcagtcggt cacgctgcgt
     3601 accgccgggt tcaatacgat tgatgagttt tccctgcggg acgccagcaa aggcgtcagc
     3661 gtagtgctga tgctgattgg cgcgggcccc gccggtacgg cgggcggctt gaagattacg
     3721 accattttta cgctcattct cgcggtgcgt tcgtatcttc gcgggcggtt cgataccgtg
     3781 gttttcggca gaacgatttc gctcgatcag gtgcgccgcg cgctgacgat tttcttcttc
     3841 gggcttgtgt ttgtcatggg catgaccatt gcgctttcgg ttgtcgagca gcataaggca
     3901 gccggcgcgc tgggaattat gaaccagctg tttgaaacca ccagcgcatt ctgcaccgta
     3961 ggcgtttcct cgggcgtgac gcaggaaagc ggaattgcca cgcgtattat tttgattctc
     4021 ttgatgtacg ccggacgcgt agggcttttg accgttgcca tgtcgctcat tgaaggcacg
     4081 acaaaggaac cggtgctgca ttacccgcag gaagatatcc agattggtta atttgcctgc
     4141 tggctgactg aacatccatg ggaaaacgga taccgtttta cttttaataa ggagtgaatt
     4201 tcatgagcaa gagctttatc atcatcgggt tgggtcgttt cggaacgagc acggcgcgca
     4261 tgctgacgac gctcggacac gaggtgctcg ttgttgacaa gcgggcggac cgcgtcaacg
     4321 gcatcaagga cagcgttatg cacgccgtac aggcggatgc gaccgacgaa cgcgcgcttt
     4381 cgcagctcgg cgtgcgcaac ttcgacaccg ttgtcatctg cattggagac gatattcgcg
     4441 cttccattct caccaccgtg ctttgccgtg agatgggcgc aaagaaaatc gttgccaaag
     4501 cgcaggacga cctgcaccag aaactgctga ttaaaaccgg cgcggaccgc gttgtccagc
     4561 cggagcacga cggcggcatc cgccttgcgc gttcgctggt tgccgagggc gttctcgatt
     4621 cgctcgacct ttcggacgag tactgcatca acg
//
LOCUS       DAOMGY010000031         4539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_19600_length_4539_cov_3.497101, whole genome shotgun sequence.
ACCESSION   DAOMGY010000031 DAOMGY010000000
VERSION     DAOMGY010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19600_length_4539_cov_3.497101"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..644)
                     /locus_tag="PLR57_00940"
     CDS             complement(72..644)
                     /locus_tag="PLR57_00940"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HQQ40049.1"
                     /translation="MNVKIVYPAVDKKRIRSYEFRSWVRWLFFLIAYACPIINIASGG
                     HAWSIVVIWSLRFIWSFTFSPDLVEYNRISQTAKLIAYSCILLILIDTFLSPGWAMFV
                     VPIFCTAGLILIGTLFFSDLTKQRQNIMPMLWLVFASIIAILSSLAGWPDRNWPMTAL
                     GATAFGILLLCVVVLGKSLLLEMEKRFHTR"
     gene            complement(634..2130)
                     /locus_tag="PLR57_00945"
     CDS             complement(634..2130)
                     /locus_tag="PLR57_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40050.1"
                     /translation="MKKVQPSRFEEYCQSLPNEKRLLLDAFFLRASDRLALDSVSNRL
                     MLRDFQEAVLWYSGRGTPLESALNLLDPVHLGGYYARPALLWYPLDDAAKIYPLSMKH
                     NSMAVFRVSICLKEAVVPALLQMALTFTIKRFPSFATTLKQGFFWHYLDAAKRRFVVE
                     PELDLPCQPLDVSGSGSQSFRVLYFEKRISVEFFHVLADGTGAMIFLNTLAAEYLRLL
                     GVAVPAGGGIKGVDASPPEEETENGFINAQSEERASGFVNKSAVQMSGRLSVAKPCRI
                     LHFHMDAERLKETAKSKNASVTAYILALMFLAQKDATDGQAGDVAVQIPVNMRKFYPS
                     GTLRNFSLYCGVRLPLRSITTLDAILPEIARQLETKASESAMKEMMSAIKQLARSLRF
                     VPLFIKKPAARIIYGFLGDRIFSSTLSNIGVIRLPDEMTPYVDHYEIVLARSSRNRAS
                     CALATYQNTTTLSIGKFTADPSFEETLYRLLSAEGLEAVVEGSALYER"
     misc_binding    2360..2620
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2704..3747
                     /locus_tag="PLR57_00950"
     CDS             2704..3747
                     /locus_tag="PLR57_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ40051.1"
                     /translation="MKKTIAILLSILMLLAAVTGCAQSAPAAEATEAPAEATATAEPT
                     AAPEEAKTYSIGIVQIVEHAALDAARDGFIQALADNGLVEGENVTFDVQNAQGDQSTL
                     STIGDRFVSANVDMILAIATPSAQTMASKTTTIPILGTAVTDYEVAKLVNSNEVPGGN
                     VSGTSDMNPIADQIALIFELYPDAATIGCIYNSGEDNSVLQAGIAKDAIEAAGKSYVE
                     VTVTSTNDVQQAMQSLVTQCDAIYIPTDNTVASAMPIVAEVANTAMIPVICGESNMVA
                     AGGLATLGINYYDLGYQTGLMALKILLEGADISTMPIEFATGFDAAFNGETAAAIGLT
                     IPEKYQSAILPAA"
     gene            3902..>4539
                     /locus_tag="PLR57_00955"
     CDS             3902..>4539
                     /locus_tag="PLR57_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40052.1"
                     /translation="MWNELTGAVGLGLIWALMTFGVFISYRILDIADLSVEGSITMGA
                     SIAAVSISAGVNPLLALLLSFLGGMAAGLITGVLHTVLKIPALLSGILTMIALWSVNL
                     HIMGKANVSLLKQATVFTPFTETLGWSKNIAVIAVAAAFVAVVFLLLYWFFGTETGCA
                     VRATGDNPNMARAQGVDTDKMKRLGLILSNGLVALAGGLIAQSQSFSDIQMGT"
BASE COUNT         1013 a         1338 c         1264 g          924 t
ORIGIN      
        1 cgttcaaaga accggaaaaa tcttcaatta ctttattttc ggcagattca gagcgaagat
       61 acgcgtataa actatctggt gtgaaatctc ttttccatct caagcagcag gcttttgccg
      121 aggacgacga cgcagagcag caatattccg aacgcggttg cacccagcgc cgtcatcggc
      181 cagtttctgt ccggccagcc ggccagcgag gataggatgg cgataatcga agcaaagacc
      241 agccagagca tcggcatgat gttctgccgc tgttttgtca ggtcggagaa gaacagcgtg
      301 ccaatgagga tcagtcccgc ggtgcagaaa atcgggacaa cgaacatcgc ccagcccggg
      361 gaaaggaacg tatcgattaa aatcagcaaa atgcaggaat aggcgatcag tttggcggtc
      421 tggctgatgc ggttatactc caccaggtcg ggggaaaacg taaacgacca gatgaaccgt
      481 agcgaccaga ttacaacgat gctccaggca tgcccgccgg aggcaatgtt gataatcggg
      541 catgcgtacg caatcaggaa gaacagccag cgcacccagc tgcggaattc gtaggaacgg
      601 atccgctttt tatccaccgc cggatacacg attttaacgt tcatacagcg cgctcccttc
      661 cacgaccgct tccagccctt cggcggaaag caggcggtac agcgtttctt caaacgacgg
      721 gtcggcggtg aacttgccaa tcgagagcgt tgtcgtgttt tggtatgtcg ccagcgcgca
      781 gctcgcgcgg tttctgctgc ttctggccag aacgatttcg tagtggtcta catacggggt
      841 catctcgtcc ggcaggcgaa tcacgccgat attggaaagc gtgctgctga aaatgcggtc
      901 gcccagaaac ccgtagatga tccgcgccgc tggtttttta ataaacagcg gaacgaagcg
      961 aagcgaacgc gccaactgct tgatcgcgct catcatttcc ttcatcgcgc tttcggaagc
     1021 ctttgtttcc agctgccgcg caatctccgg caggattgca tccagcgtcg taatcgagcg
     1081 caacggcaga cgaacgccgc agtagagcga aaagttccgc agcgtaccgg aaggataaaa
     1141 ttttcgcatg ttcacgggaa tctgcacggc aacgtccccc gcctgcccat ccgttgcgtc
     1201 tttctgcgct aggaacatca gcgcaaggat atacgccgtt acggaagcgt tttttgattt
     1261 cgcggtctct ttgagccgct ccgcatccat atgaaagtgc agaatcctgc agggcttcgc
     1321 cacggagagc cggccgctca tctgtacggc ggatttgttg acaaagccgg acgcgcgttc
     1381 ttccgactgc gcattgataa atccgttctc ggtctcttct tccggcggag aagcgtctac
     1441 gccctttatg cctccgccgg cgggcaccgc aacgccgagc aggcgcagat actccgccgc
     1501 cagcgtgttt aaaaagatca tcgcgccggt tccgtccgcc aatacgtgaa aaaactccac
     1561 gctgattcgc ttttcgaaat acagtacgcg gaaggactgc gacccgctgc cggagacgtc
     1621 cagcggctgg caaggcaaat ccaattcggg ttcaacgacg aaccgccgct tcgccgcgtc
     1681 cagatagtgc cagaaaaagc cctgcttcag cgtcgtggca aagctgggga atcgcttaat
     1741 tgtaaacgta agcgccatct gcagcagcgc gggcacgacc gcttccttca ggcagattga
     1801 aacgcgaaat accgccatgc tgttgtgctt catcgagagc ggatagatct ttgccgcgtc
     1861 gtccagcgga taccagagca gtgcgggacg cgcgtagtat ccgcccagat gaaccggatc
     1921 gagcaggttc agcgcgcttt ccagcggcgt tccccgcccg gaataccaca agaccgcttc
     1981 ctgaaagtcg cgaagcatca agcggttgct gacggaatcc agcgcaaggc ggtcgctggc
     2041 gcgcaggaaa aacgcatcca gaagcaaccg cttttcgttt ggcagagact ggcagtattc
     2101 ttcaaatcgg gaaggctgta cttttttcaa gatctgttta ctcccctgtc cggcgcgtcc
     2161 ggaagagccc ggcgcacgcc gcgttcgtag gttcgaataa gtaatcatat catttttata
     2221 cgcggtaaaa aagcattccg atttgaatta cgccaggttt gccgcgcggc ggataaattt
     2281 ctggccgtaa ctctgatatc tctgcggaaa attcggcttg acaagccata tataatatat
     2341 tataattccc cacaacacga aaaaggcagc gaccgggaat aagtacgcat cgggttttgc
     2401 gcaaagagag ctttcggctg gtggaagaaa gcggcaagga cgataccgaa ttcatctcga
     2461 gagccggcgg ttgaaagaga gcgggagcgc ttctcctgta ggccgatacg ggtacgcccg
     2521 ttacagcgcg gggcggttgt tatgaccgcc gaccgagtcc ctgttctgcc gcaaggcgca
     2581 gggagaaaaa aggtggtacc gcgatcatct cgcccttttc cggaaatgcc ggagaaggac
     2641 gtttttttat ccccgcggtt gcggcggaga tagagcattc taaaaaacgg aggaaatcga
     2701 attatgaaaa aaaccattgc catcctgctc agcatcctga tgctgcttgc ggcggtaacc
     2761 ggctgcgcac agagcgcgcc cgccgccgaa gccaccgaag cgcccgcaga agccaccgcc
     2821 accgcggagc ccaccgcagc gccggaagaa gcgaaaacct acagcatcgg catcgttcag
     2881 attgttgaac acgccgcgct ggacgccgcc cgcgacggat ttatccaggc gctggcggac
     2941 aacggccttg ttgaaggcga aaacgtcacc ttcgacgtgc agaacgcgca gggcgaccag
     3001 tccacccttt cgaccatcgg cgaccgcttt gtgagcgcga acgtggatat gatccttgcc
     3061 atcgcgacgc cttccgcgca gaccatggcc agcaagacca ccaccatccc gatcctcggc
     3121 accgccgtga ccgattatga agtggccaag ctcgtcaact ccaacgaagt gcccggcggc
     3181 aacgtctccg gcacgtcgga catgaacccc attgccgacc agatcgcgct gatctttgag
     3241 ctgtatcccg acgcggcgac catcggctgc atctacaaca gcggagagga taactccgtc
     3301 ctgcaggcgg gcatcgccaa ggatgccatc gaagccgcgg gcaagagcta cgtcgaagtc
     3361 accgtgacct ccaccaacga cgtccagcag gccatgcagt cgctcgtcac ccagtgcgac
     3421 gcgatctaca tcccgacgga caacaccgtt gcctccgcca tgccgatcgt tgcggaagtg
     3481 gccaacaccg ccatgattcc ggttatctgc ggcgaatcca acatggttgc cgccggcggc
     3541 cttgcgaccc tcggtatcaa ctactatgac ctcggctacc agacgggcct catggcgctg
     3601 aagattctgc tcgaaggcgc ggatatctcc accatgccca tcgagttcgc aaccggcttc
     3661 gacgccgcct tcaacggcga aaccgccgcg gccatcggac tcaccattcc cgaaaagtac
     3721 cagtccgcga ttctgcccgc cgcgtaagcg aacaagcgga aaacgcacga tacctaactt
     3781 gctgacaagg caagcgctcg cggaaccatt ccgcgcgcgc ttgcctgcgt ttggcaaaag
     3841 aaccaagttt ttctttatcg agttttttct ttatcgaatt ttttcattga ggtggtaaac
     3901 catgtggaac gaattgaccg gcgcggtcgg tctggggttg atctgggcgc tgatgacttt
     3961 tggggtgttc atcagctacc gcatcctcga tattgcggac ctctccgttg aaggcagcat
     4021 cacgatgggc gcttccatcg cggcggtcag tatctccgcg ggcgtcaacc cgctgctggc
     4081 gctgcttctc tcctttcttg gcggcatggc tgcggggctt atcacaggcg tgctgcacac
     4141 ggtgctcaaa attcccgcgc tgctctccgg cattctgacc atgatcgcgc tctggtcggt
     4201 caacctccat atcatgggca aggcgaacgt ctcgctgctc aagcaggcaa cggtgtttac
     4261 gccgtttacc gaaacccttg gctggagcaa gaatatcgcc gtcatcgcgg ttgccgccgc
     4321 gttcgtcgcg gtggtgtttc ttcttcttta ttggtttttc ggcacggaga ccggctgcgc
     4381 cgtccgcgcc acgggcgaca acccgaatat ggcgcgcgcg cagggcgttg ataccgacaa
     4441 aatgaagcgg ctggggctga tcctttccaa cgggctcgtc gctctcgcgg gcggcctgat
     4501 tgcgcagagc cagtcgtttt cggatattca gatgggcac
//
LOCUS       DAOMGY010000032         4527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_19681_length_4527_cov_3.328041, whole genome shotgun sequence.
ACCESSION   DAOMGY010000032 DAOMGY010000000
VERSION     DAOMGY010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19681_length_4527_cov_3.328041"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="PLR57_00960"
     CDS             <1..805
                     /locus_tag="PLR57_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40053.1"
                     /translation="PLVSSGGGTRPLFDEAERKAADHVTMFDEIALDPEHPLPDGVIT
                     VTETIRIAEGDAMSDTTALAKIVHTFTFDTTAGNAAPATADELRIPLSGETYLSVYHR
                     EMGADGVNRMTSVSTEKVSLDGVVLSMTTEYLSTGTQVEIRIAEAPERWTEDMKTSLL
                     FMTWPNTDGAITSAGVSATLMIDGEPAGDLPMPDRWGEAELLYLLPIFQGGESPVSSA
                     VLQLSYSYYTKLNDTDQLAGETLTIPEDGLEFFGVTTTVPLTEITIPLP"
     gene            complement(875..1192)
                     /locus_tag="PLR57_00965"
     CDS             complement(875..1192)
                     /locus_tag="PLR57_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40054.1"
                     /translation="MLAGRQSQRCVEVWRETMPYLERHNETDEVFVLLEGACTLCIGY
                     DDQSPGKIELFVMEKNKLYNVKHEVWYNLTAVAGTTLLIMESTDTSEVNSNYFSVTLE
                     MLP"
     gene            complement(1271..2227)
                     /locus_tag="PLR57_00970"
     CDS             complement(1271..2227)
                     /locus_tag="PLR57_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HQQ40055.1"
                     /translation="MNAYILEINGRHAAALTEDGRFVRVKNKNYFVGQSVKLFERKAE
                     TRQRVRYSALASMAAGFALLLFGGFAGYNAPVGVVSLDVNPSIEYTINCFDRVLAIEG
                     VNEDGSRIISEMNSENLINQPVDEAVEATILQLRESGYLAEQSENDVVLSASALTLTH
                     ANALANRLEQLVMQQEDLQVTSISVSKSEVQQAHALGTSAGKLYLIERLQQATGTDEA
                     FDPKDWLNTPVREIIRETATRSGAPQQNGEQNGQTPAGSQSPDSTTPQNSTGTPVPGG
                     STTSPQPTGGEQGSPVGPGPTDGSGGTGGAGGEPPAGGSPPG"
     gene            complement(2224..2943)
                     /locus_tag="PLR57_00975"
     CDS             complement(2224..2943)
                     /locus_tag="PLR57_00975"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HQQ40056.1"
                     /translation="MAQDESAAALRAAKDERQREALIQRQERNILRIASRAKHRFVTK
                     SDDEWSVALCAFSRAIDSYVPERGAFLPYAETLIKRSLIDAYRSEARRENEFTVPPEA
                     FDGETDEDGENPVLGAVVQMSFQRQDTRLKDEIAAVSAQLKLFGFSFFDLTECSPSQQ
                     RTRSACAKAVRALLARPEDLDAMFRTRKLPMKNLIRFDGVSGKLLGRYRKYIIASLVI
                     LTGDYPILGAYIRGSERGEEE"
     gene            complement(3078..3974)
                     /locus_tag="PLR57_00980"
     CDS             complement(3078..3974)
                     /locus_tag="PLR57_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40057.1"
                     /translation="MQNRNKAKSIGRLSGRAKRVLAVLAVFILTMSFGASALADGGRG
                     NGAPSGAPAGGQQTPGVTGGQQPENGNGEQNGNPSREATGANLDKIEAAIATIEDETI
                     QASLTALLEAYADALEAKQAAIEANDTASISELAAAASAAKDALDLAMTEAGLSLDDI
                     LSTPLQAEDGTGRMLGKPELDTESIAAEIAALDDSDENKAVLEGLLNAYKVALQARVS
                     ANTAAMSEGEIAALDEAVTAAEVELREKLANAGLSTELTVQNQQEQQSQWQLSVIPDG
                     TEAPSGTGFFATIFNWLNSLFK"
     gene            complement(4225..>4527)
                     /locus_tag="PLR57_00985"
     CDS             complement(4225..>4527)
                     /locus_tag="PLR57_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40058.1"
                     /translation="GWGTAGANGGTCAFTVSGQTMNGNILVDEISSLDFSMLSGSVYT
                     GAINPSGAAGTVNVTIEDGCQWILTGDCYITSFTGSVANIVTNGYAVYVNGVAITG"
BASE COUNT          861 a         1418 c         1243 g         1005 t
ORIGIN      
        1 cccgctggta tccagcggcg gcggaacgag gccgctcttt gacgaagcgg agcggaaagc
       61 cgccgatcac gtgaccatgt tcgacgaaat tgcgctcgat cccgagcatc cgctgccgga
      121 cggcgtcatc accgtaaccg agaccatccg cattgcggaa ggcgacgcaa tgagcgatac
      181 caccgcactg gcaaagatcg ttcacacgtt caccttcgac accacggcgg gcaacgccgc
      241 gccagccacg gcggatgagc tgcgcattcc gctctccggg gaaacgtacc tttcggttta
      301 tcatcgggag atgggcgcgg acggcgtaaa ccgcatgacg tcggtttcca ccgaaaaagt
      361 ctcgctcgac ggcgttgtgc tgagtatgac cacggaatac ctttccaccg gcacgcaggt
      421 ggagatacgc attgccgaag cgccggaaag atggacggag gacatgaaaa ccagcctgct
      481 gttcatgaca tggccgaaca cagacggcgc gattacctcc gccggcgtca gcgcaacgct
      541 catgatcgac ggcgaacccg cgggcgactt gccaatgccc gaccgctggg gcgaggcgga
      601 gttgctgtac ctgctgccga tcttccaagg tggtgaaagt cccgtctcct ccgccgtgct
      661 acagctgagc tactcctatt acacaaagct caacgatacg gatcagctgg ccggagaaac
      721 gctcactata ccggaagacg ggctggagtt tttcggggtc acgactacgg ttccgctcac
      781 agagatcaca attcctctgc cgtaatcaaa cggcatgaaa cgcccgcacg aagtatcgcg
      841 cgggcgtttt gctttatttg atttgtttcg gtttttacgg cagcatctca agtgtaacgg
      901 agaaatagtt cgaattcacc tccgaggtgt ccgtgctctc catgatcaaa agcgttgtac
      961 ctgccaccgc tgtcaggtta taccaaacct cgtgcttgac gttgtagagc ttatttttct
     1021 ccatgacgaa cagctcgatt ttaccgggcg attgatcgtc atagccaatg cagagcgtgc
     1081 acgcgccttc cagcagcacg aacacctcgt ccgtttcgtt atgccgttcg agatacggca
     1141 tcgtctcgcg ccaaacttcg acgcatcgtt gagactggcg acccgccagc acagccgctc
     1201 tcctatgcag aaaatccccc gcggggcgct ttgtccgcgg gcgcaagttc attccgttta
     1261 ccgggaaaaa tcagcccgga ggacttcctc ccgcaggcgg ctctccaccc gcgccccccg
     1321 ttccgcccga accgtccgtc ggcccggggc caacgggact gccttgctca ccgcctgttg
     1381 gttgcggcga tgtggtgctt ccacccggca caggcgtacc cgtactgttt tgcggcgtag
     1441 tagaatcagg cgactgcgac ccggctggcg tttgtccgtt ctgttcgccg ttttgctgcg
     1501 gcgcgcccga acgggtggcc gtttcgcgga tgatctcacg aacgggcgtg ttcagccagt
     1561 ctttcgggtc gaacgcttcg tccgtcccgg tcgcctgctg caaccgttca ataagataga
     1621 gcttgcccgc gctggtgccg agcgcgtgcg cctgctgcac ttcgcttttc gaaacggaaa
     1681 tggaagtcac ctgcaaatct tcctgctgca tgacgagctg ctccaaacgg tttgcaaggg
     1741 cgttggcatg cgtaagcgtc agggcgcttg ccgaaagcac gacgtcgttt tccgactgct
     1801 ccgcaaggta cccgctctcg cgcagctgga gaatggtcgc ctcaaccgcc tcgtccacgg
     1861 gctggttgat taggttttcg gaattcatct cgcttatgat tctgctgccg tcctcgttga
     1921 cgccttctat ggcgagcacg cggtcgaagc aattgatcgt gtattcgatg gatggattta
     1981 cgtccaaact gaccaccccg acgggcgcgt tatagcccgc gaacccgccg aacagcagca
     2041 gcgcaaatcc ggccgccatg gaagccagcg cggaatatcg cacgcgctgc cgcgtctccg
     2101 ctttccgctc aaacagcttc acagactggc cgacaaaata gtttttattc tttacacgaa
     2161 caaacctgcc gtcctcggtc aaagccgcgg cgtgtctgcc gttaatttcc aatatatagg
     2221 cgttcattct tcttcccccc gctcgctgcc gcggatatac gctccaagaa tgggatagtc
     2281 ccccgtgagg atgaccaatg aagcgatgat gtactttcgg tagcggccca atagtttccc
     2341 gctgacgccg tcgaaacgga tgagattttt catgggcagt tttcgcgttc gaaacatcgc
     2401 atccaaatcc tcgggccttg caaggagcgc gcgcaccgcc tttgcgcagg cggatcgcgt
     2461 ccgctgctgg ctcggcgagc actctgttaa gtcaaaaaag ctgaatccaa acagttttag
     2521 ctgagcgctc actgccgcga tttcgtcttt cagccgggta tcctgtctct gaaagctcat
     2581 ctgcacgacc gcaccgagca ccggattttc gccgtcttcg tccgtttccc cgtcgaacgc
     2641 ttccggcggc acggtaaact cgttttcgcg ccgcgcttcg ctgcggtaag cgtcgatgag
     2701 gctgcgcttg atcagcgttt ccgcatacgg cagaaaagcg cctcgctcag gaacgtagga
     2761 atcgatcgcg cgggaaaagg cgcacaaagc aaccgaccat tcgtcgtcgc tcttcgttac
     2821 aaagcggtgc tttgcgcggg aagcgattct tagaatgttc ctctcctgcc gctgtatgag
     2881 cgcttcgcgc tggcgttcgt cttttgccgc cctcagcgcc gctgccgatt cgtcctgcgc
     2941 catgttctcc tgtcgttcgt tgttaatttg ccttacgtgc cgtctgccgg ttccccgtca
     3001 aactggcttc tgtgccgctc gccctccgca agccgcgggg aaacggattc cccgcagcct
     3061 gcgaaagtcg tcaggcatta cttaaagagg gagttcagcc agttgaagat tgtagcaaag
     3121 aatcccgtac cggacggcgc ttccgttccg tccggaatga cggaaagctg ccactgcgac
     3181 tgctgttcct gctggttctg cacggtaagc tccgtggata aaccagcgtt tgccagtttc
     3241 tcgcgcagtt ccacttcggc ggctgtcacc gcttcatcca gagccgcgat ctcgccctcg
     3301 gacatagcag ccgtgtttgc ggaaacgcgc gcctgcagcg caaccttgta agcgttcagc
     3361 aggccttcca gcactgcttt gttctcgtcg gaatcgtcca gcgcggcaat ttcagccgcg
     3421 atcgattccg tgtccagttc gggcttgccg agcatgcggc ccgtgccgtc ctcggcctgc
     3481 agcggcgtgc tcaggatgtc gtccagtgaa agccccgcct ccgtcatggc aaggtcgagc
     3541 gcatcctttg ccgccgaagc agccgctgcc agttcggaaa tgctggccgt atcgttcgct
     3601 tcaatggcgg cctgcttggc ttccagcgcg tccgcatacg cttccagcag cgccgtcaaa
     3661 ctcgcctgaa tcgtttcgtc ctcaatcgtg gcgatggcag cttcgatctt atccagattg
     3721 gcacccgtcg cttcgcgcga aggattgccg ttctgttcgc cgtttccgtt ttcgggctgt
     3781 tgtccgcccg taacaccggg ggtctgctgt ccgcccgcag gcgcgccgct tggagcgccg
     3841 ttgcctctgc cgccgtcggc cagcgcggac gcaccgaacg acatggtcag aatgaatacc
     3901 gcaagaacgg ccagcacgcg ctttgccctg ccggacagtc tgccgatcga ctttgctttg
     3961 tttcgattct gcatcgtatc atctcctgta tcgtaaattt ccgccggcgt ttgccggtca
     4021 ctagagttct acgatgcaag agggtgcaaa agtggggtca gacctccaaa ttcgcggttc
     4081 ttttcatttc gttcacattg tgaggcgtgt attcccccat ttcacaccga ataagaatgg
     4141 aaactcctcg ccaaaaaacg gcaaagcgcg gagcgcgccg atttggcaca ctccgcgctc
     4201 attaccagtt acatcatatc ccgatcagcc ggtaatcgca acaccattga cgtaaacggc
     4261 atagccgttg gtcacgatat tggcaacgct gcccgtaaac gaggtgatat agcagtctcc
     4321 cgtgagaatc cactggcagc cgtcctcaat ggttacgttc accgttcccg ctgcgccgga
     4381 ggggttgatt gcgcccgtgt acaccgagcc gctcagcatt gaaaagtcga gcgaagaaat
     4441 ctcgtccacg aggatattgc cgttcatcgt ctgcccgctg actgtaaagg cgcaggttcc
     4501 gccgtttgcg cccgccgttc cccagcc
//
LOCUS       DAOMGY010000033         4498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_19853_length_4498_cov_3.158902, whole genome shotgun sequence.
ACCESSION   DAOMGY010000033 DAOMGY010000000
VERSION     DAOMGY010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19853_length_4498_cov_3.158902"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PLR57_00990"
     CDS             complement(<1..678)
                     /locus_tag="PLR57_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HQQ40059.1"
                     /translation="MKKTFRGGVHPLRKNGEGKPLTNACSVRAVVPDVVVIPMGMHLG
                     APGAPCVQKGQRVKLGEVIATPVGALGLPVHASVSGEVLFVEPRQQLRAAPENCVGIK
                     NDGLDEWVELTPAGTLESVTPEQIIAAVKNAGICGMGGAAFPTHIKLSVPEGKHADTV
                     ILNGAECEPYLTADHRLMLETPERIVSGLKLILKATGVSRGVIAIESNKPDAIESVRA
                     AAESAAGI"
     gene            complement(930..1802)
                     /locus_tag="PLR57_00995"
     CDS             complement(930..1802)
                     /locus_tag="PLR57_00995"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQQ40060.1"
                     /translation="MKKIVKRRRTFRINNPLAFGILCAMILIMLIGVSYAIVAGVISP
                     AIVKYQVANNTPTPAPTVAPVTATPSPTVDPALPTPTPSLEPGSTPTPTIPPAGKLEG
                     HVIGIDPARGYSSKIQGVSTKIYANRLNYAVATLVKEMLEEEGATVVFSLSSVKDDKT
                     ATERAAIFNSGGVEFALRIECNYVNATDTRGAMAWIPETHDQKASCSKLASAVLNAYI
                     DATGLPLREVKGESIIEKSDETFFNKTEAPVCTLFIGHISNATEDKKINDADFQKTMA
                     QGIVNGILSYLGFK"
     gene            2170..3030
                     /locus_tag="PLR57_01000"
     CDS             2170..3030
                     /locus_tag="PLR57_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HQQ40061.1"
                     /translation="MPYNYTHALVGLTALENADDTVKALVGKHKGSFLIGTMGPDPYF
                     GDEMPKPLFVPCRKPVADKLHTMDMRSIAAELLPLAAQNPASQAYALGFLCHFLLDTN
                     AHPYIEARFSGKAHTPAEIQIDLMMTDRIKRPGVPLPPARFYRTRDTAALDKLHTSLI
                     RQLLSLEIKGSFSRSLQKWIAVNTVSFDPNNRKLRFFSRLDHSGKLTGFLVARHPDPD
                     DRLNLGHQEWRAPWAPEQARTESFPDLFDRAVKEAPSLLNAAYAAMQSGDTTPALALI
                     GPRRMDAKPL"
     gene            3014..3553
                     /locus_tag="PLR57_01005"
     CDS             3014..3553
                     /locus_tag="PLR57_01005"
                     /inference="COORDINATES: protein motif:HMM:NF018601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG family protein"
                     /protein_id="HQQ40062.1"
                     /translation="MQSLSEFDCSSLRACCFTGHRPESLPDRGNEEKRGMRELQSLLE
                     AAVFRALEDGITLFFAGGARGFDTLAAETVLRCRETNPDVTLFLALPGLDQTEDWPEG
                     DRLRYEAILSAVGNCVWYAADRCSPDSMRKRNRYLVDHSTRCIAYLKRMRGGTLYTVN
                     YALENDVPVENLALFMPRV"
     gene            3736..>4498
                     /locus_tag="PLR57_01010"
     CDS             3736..>4498
                     /locus_tag="PLR57_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQQ40063.1"
                     /translation="MGVLPEGRLLLSMGVPLALSMLTQALYNIADSIFVSFIDESALS
                     AVTLSYPIFMLMISVAVGTGVGINSLISRRLGAKRFAEAELAASNGFFLMLFSSLLFV
                     VFGLFFAKPFISAYTSTPETAAYGADYLSIVSTFSVGLFIQVFCERIMQSQGKNLYSM
                     LMQLVGAVVNIVLDPILIFGLLGFPKLGVAGAAIATVTGQMTSMVFSMFVIFGKKNEV
                     RLTLKGFKPDLASIKGIYEVGFPTIILQAIGTVMTL"
BASE COUNT          907 a         1294 c         1219 g         1078 t
ORIGIN      
        1 tatcccggcg gcggactctg cggccgcgcg tacggactcg attgcgtccg gcttgttcga
       61 ttcaatcgcg ataacgccgc ggctcacccc tgttgccttg agaatcagct tcaggccgct
      121 gacgatacgc tccggcgttt ccagcatgag ccggtggtcg gcggtcagat acggctcgca
      181 ttccgcgccg ttaaggatga ccgtgtccgc atgcttgcct tccggaacgg atagctttat
      241 atgcgtggga aacgcagctc cgcccatgcc gcagataccc gcgttcttta cggcggcgat
      301 aatctgctcc ggcgtgaccg actccagcgt acccgcgggt gtcagttcaa cccattcgtc
      361 cagaccgtcg tttttgatgc cgacgcagtt ctccggcgcg gctctgagct gctggcgggg
      421 ttccacaaac agaacttcgc cggacacgct cgcgtgaacg ggaagcccga gcgccccgac
      481 gggagttgcg attacttcgc ccagcttcac gcgctgcccc ttctgcacgc agggagcgcc
      541 cggcgcgcca agatgcatcc ccatcgggat caccacgacg tccggcacga ccgcgcgcac
      601 agagcaagcg ttagtcagcg gttttccttc gccgtttttt cggagaggat ggacgccgcc
      661 gcgaaacgtt tttttcaatc gattccacct gccttgtatt aatcgggctt agataaggaa
      721 gattgcccgt ttgaaacctc atacagtata aggtatttgc cgctccgaaa caagtgccgc
      781 ggctacagag tattagaaat tcagcggaat tcagcgcttt gaagagccgc agaacgtcaa
      841 caagcgtaaa aaacgttttg tttacgttta tcgcctttgt caatgaaaaa aaccgccggg
      901 aaacccgacg gttcttcatt attaattgct tatttgaagc caaggtagct caatatgccg
      961 ttgacaatgc cttgcgccat ggtcttttgg aagtccgcgt cgttgatctt tttatcctcc
     1021 gtggcattgc tgatgtggcc gataaagagc gtgcagaccg gcgcttcggt cttgttgaag
     1081 aacgtttcat ccgatttttc tatgatggat tcgcccttca cctcgcgcag gggaagcccc
     1141 gttgcgtcga tataagcgtt cagcaccgcg ctggccagct tgctgcagga ggccttttga
     1201 tcgtgtgtct ccggtatcca ggccatggct ccgcgcgtat ccgttgcgtt gacatagttg
     1261 cattcaatgc gaagcgcaaa ctccacgccg ccgctgttga agatggctgc gcgctcggtt
     1321 gcggtcttgt cgtccttgac gctgctgagc gaaaagacga ccgttgcgcc ttcttcctcc
     1381 agcatctctt tgaccagagt ggctaccgca taattcagcc tgttggcgta gattttagta
     1441 gaaacgccct gtattttcga gctgtatcca cgcgccggat cgataccgat tacgtgccct
     1501 tccagctttc cggcgggcgg tatggtcggc gtcggggtgg aaccgggctc cagagaaggc
     1561 gttggcgtag gcagcgcggg atcgaccgtt ggagagggcg ttgccgtaac gggagcgacg
     1621 gtgggagcgg gcgtaggcgt gttgtttgcg acttgatatt tcacgatagc gggtgaaatt
     1681 acaccggcga cgattgcata actgacgccg atgagcataa tgaggatcat agcgcataga
     1741 attccgaatg cgagcggatt attgatgcga aacgttctcc tgcgcttgac aatctttttc
     1801 atgctctcca cctcagtcgt gctatcaagg ttattgtaac acgctcgtaa gagaatgcac
     1861 catagaaaaa cataagattt tcaaaattta cacgttttcg ttagaaaaaa ttcatgtctt
     1921 tttgaacgaa ttgcgataca gcgaatttgt gtgatttttg tgggctcccc ttccctgtca
     1981 agtgtgccgt atagggcagc ggcatttccg ttacctcgta attcagcgtg taaaattcac
     2041 cggattcgag ctgaaaccgc accgtctcca tgctctgaaa agcaattcaa cgatacggat
     2101 ttgcactgat ttaagttgtt ttttgtcgaa tgcttgaacc cttgccccgc atgaacgtat
     2161 aatactgtta tgccatataa ttatacacac gcgctggtgg ggctcaccgc gctggagaac
     2221 gcagacgaca ccgtaaaggc ccttgtcggt aagcacaagg gttcgttttt gatcggcaca
     2281 atggggcccg atccgtactt tggagacgaa atgccaaagc cgttgttcgt tccctgccgc
     2341 aaacccgtgg ccgacaaact gcacacaatg gatatgcgat caatcgcggc agagttgctt
     2401 ccgctggccg cgcaaaaccc tgcgtcgcaa gcgtacgcgc tcgggttttt atgccacttc
     2461 ctgttggata ccaacgcgca tccttatatt gaagcgcggt tttccggcaa ggcgcatacg
     2521 cccgccgaaa tccagatcga cctgatgatg accgatcgta tcaagcgccc cggcgttccg
     2581 cttccgcctg ctaggtttta ccgcacgcgg gataccgccg cgctggacaa gctccataca
     2641 agtctgatcc gccagctcct ttcgctggag atcaagggct cgttctcccg cagtctgcag
     2701 aagtggatcg cggtcaacac ggtgtcgttt gacccgaaca accgcaagct ccgctttttc
     2761 agcaggttgg atcacagcgg caagctgacc ggttttctcg ttgcccgcca tcccgacccg
     2821 gacgaccgcc tcaacctcgg tcaccaggag tggcgcgcgc cctgggcgcc ggagcaggcg
     2881 agaacggaat ccttccccga cctgttcgac cgcgcggtaa aggaagcgcc ctcgctgctc
     2941 aacgccgcgt acgccgccat gcagagcggg gataccaccc ctgcgctggc cctgatcggc
     3001 ccacggagga tggatgcaaa gcctctctga atttgactgc tcctctttac gcgcctgctg
     3061 ctttaccggt caccgtccgg aatcgctgcc tgatcgcgga aacgaagaaa agcgcggcat
     3121 gcgggagctc cagagccttc tggaagccgc cgtttttcgc gcgctggaag acgggattac
     3181 cctctttttc gcgggcggcg cgcgcgggtt tgatacactc gccgcagaaa ccgtgctgcg
     3241 ctgccgcgaa acgaaccccg acgtaacgct gtttctcgcg cttcccggac ttgatcagac
     3301 cgaggactgg ccggaaggcg accggctcag gtacgaggcg attctctccg cagtgggaaa
     3361 ttgcgtgtgg tatgcggcag atcgttgcag tccggattcg atgcgtaagc gcaaccgcta
     3421 tctggtggat cattccaccc gctgcatcgc ttatttaaag cgcatgcgcg gcggcacgct
     3481 ctatacggtg aactacgcgc tggaaaacga cgttcctgtt gaaaatctcg ccctgttcat
     3541 gccgcgtgta taacgaaaca cgtattattc gcgcttttat taccgcctgc cgcccggcag
     3601 gcgttcttgt ttgaaccgaa ttgatattta tttgattgta caataatttt tatcgtggta
     3661 taattcgtaa ccgttatgga acagaaaaat tcgacagctt caaaccggcc ctgcgcacca
     3721 gcgcagccga acaaaatggg cgttctgccg gaaggcaggc tcctgttgtc catgggcgtg
     3781 ccgcttgcgc tctccatgct cacgcaggcg ctctacaaca ttgcggacag cattttcgtt
     3841 tcgttcatcg acgaaagcgc gctctcggct gttacgctga gctacccaat ctttatgctg
     3901 atgatatccg ttgcggtcgg caccggcgtc ggcatcaact cgctgatttc gcgcaggctc
     3961 ggcgcaaagc gtttcgccga agcggagctc gccgccagca acggattttt cctgatgctg
     4021 ttctcctccc tgctgttcgt ggtgttcggg ctcttttttg ctaagccctt catttcggct
     4081 tacacgagca caccggagac ggcggcttac ggcgcggact acctttctat cgtttccacg
     4141 ttcagcgtgg gcctgtttat tcaggtgttc tgcgagcgca tcatgcagag tcagggcaaa
     4201 aacctttact ccatgctcat gcagctcgtc ggcgcagtgg tcaacatcgt gctcgacccc
     4261 atcctcatct tcggcctgct gggcttcccc aagctcggcg tggcgggcgc tgccattgca
     4321 accgtcaccg gacagatgac ttccatggtc ttttcgatgt tcgtcatctt cggcaaaaag
     4381 aacgaagtgc gcctcacctt aaaaggtttc aagcccgatc ttgccagcat caagggtatt
     4441 tacgaggttg gtttcccaac gatcatcctg caggccatcg gtacagttat gacgctgg
//
LOCUS       DAOMGY010000034         4472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_20028_length_4472_cov_4.093955, whole genome shotgun sequence.
ACCESSION   DAOMGY010000034 DAOMGY010000000
VERSION     DAOMGY010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20028_length_4472_cov_4.093955"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /gene="sdaAA"
                     /locus_tag="PLR57_01015"
     CDS             complement(<1..736)
                     /gene="sdaAA"
                     /locus_tag="PLR57_01015"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HQQ40064.1"
                     /translation="MLLSEAFENGESLRKYCLENGIPIYEAMIRREEKQSERSREEIL
                     EEMRLNLRVMKESVYRGLNEKVESVSGLSGGEAMRLFKYGKHNPFSGYTACRAAASAM
                     AVVEVNASMGRIVAAPTAGASGILAGALIESGRQRGWSDDTLVEGLFTAGAIGFIYAK
                     NATISGAGGGCQAETGVAASMTAAALVEISGGAPVQALDAAAMAMKNVMGLICDPVAG
                     LVECPCIKRNALGAVNAMLCADMALAA"
     gene            complement(750..1412)
                     /gene="sdaAB"
                     /locus_tag="PLR57_01020"
     CDS             complement(750..1412)
                     /gene="sdaAB"
                     /locus_tag="PLR57_01020"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HQQ40065.1"
                     /translation="MEDFSVFDIIGPRMTGPSSSHTAGAARLSRVARHIAEDDVAEVK
                     FTLYGSFAETGRGHGTDKALIAGALGMEPDDPRIKDAYRVAREMGVLVTMEFSEEQPA
                     FPNTARIVITGSNGQTTDVVGESVGGGNIRITEINGLAVEFSGEYPTLVIQHTDKPGV
                     IAEVSHVLAQLSVNIAFMRVFRHGKGEDAYMTIETDQPVTRDMQAMILKLCSGIIKLF
                     AV"
     gene            complement(1505..1772)
                     /gene="ffs"
                     /locus_tag="PLR57_01025"
     ncRNA           complement(1505..1772)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLR57_01025"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(1888..2793)
                     /locus_tag="PLR57_01030"
     CDS             complement(1888..2793)
                     /locus_tag="PLR57_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HQQ40066.1"
                     /translation="MNIREMAKEFGFAEAFVFSTEPFTYFERRLEDGALHHSGTSLTA
                     DVHAVYSWANAILALILPYCPYPDSVPVSGNYPSSNKAYHASNKLIRALNEQGIQAKR
                     AYIPVRELLIRNGVGVPLKNGLTYIPGYGTRYSVQTLLIDLPEVSYTPVQTQEEPFCT
                     KCHACERVCPSGAISHNGYDYKTCARAYMGGDPMEDWVMDAMTSILGCELCQSICPYN
                     REIEPVSDVPEVFALEKLLSGDVKPALKIVGTNLNKNGRLIQHACVMAAKLGRKDLLP
                     LIEKLLEDQREGVRAAAAYAVKRLR"
     gene            complement(2790..3422)
                     /locus_tag="PLR57_01035"
     CDS             complement(2790..3422)
                     /locus_tag="PLR57_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HQQ40067.1"
                     /translation="MAAIQVKPTRMELSNLKKRKKVAVRGHKMMKDKRDELVRRFIEY
                     ARENKRLRADVEEKLSRAMKSFVLARASMSGAEIEEALIYPARALDVTAGIEHVLSIQ
                     VPKLAISAQEGFTYPYGFATTSADLDMAVQLLAEVLPLLVQLAEIEKACGRLADEIEK
                     TRRRVNALEYVMIPQFTETIRRIQMKLEENERGNLTRLMKTKEMLQEQGR"
     gene            complement(3426..>4472)
                     /locus_tag="PLR57_01040"
     CDS             complement(3426..>4472)
                     /locus_tag="PLR57_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HQQ40068.1"
                     /translation="INGTPMNPTARDYPSEFIQTGISAIDGLNTLVRGQKLPVFSGSG
                     LPHANLAAQIARQAKVLGSDEKFAVVFAAVGITFEEADFFISDFRKTGAIEKTVTFIN
                     LANDPAIERIATPRMAITAAEYLAYELGMHVLVIITDITNYAEALREVSAARKEVPGR
                     RGYPGYLYTDLATMYERAGRIRENAGSITMIPILSMPEDDVTHPIPDLTGYITEGQII
                     LSRELYRKGLTPPINVLPSLSRLKDKGIGKGKTREDHADTMNQLFSAYSRGKDAKELA
                     VILGDAALSETDKLYAKFADAFEEQYVSQGYYTNRTIEETLDLGWKLLGILPKSELKR
                     IRDAYIEKYYPKEA"
BASE COUNT          878 a         1313 c         1255 g         1026 t
ORIGIN      
        1 gcgcggccaa tgccatatcc gcgcagagca tggcgttgac cgcgccgagc gcgttgcgct
       61 tgatgcaggg gcactcgacc aagcccgcca cggggtcgca gatcagcccc attacgtttt
      121 tcatggccat ggccgccgca tccagtgcct gcacgggcgc tccgccgctg atctcaacga
      181 gcgccgccgc cgtcatgctc gccgcaacgc ccgtttccgc ctggcacccg ccgcccgcac
      241 cgctgatggt cgcgttcttg gcgtaaataa acccgattgc gccagccgta aacaagcctt
      301 ccacgagcgt atcgtccgac cagccgcgct ggcgcccgct ctcgatgagc gcgcccgcga
      361 ggatgccgct cgctcccgcc gtgggagcgg cgacgatacg ccccatagat gcgttcactt
      421 cgactaccgc catggcggaa gccgctgccc gacaggcggt gtatccggaa aacgggttgt
      481 gcttgccgta tttgaacagc cgcatcgcct ctccgccgga aagcccgctc acggactcga
      541 ctttctcgtt cagcccgcga tagacggatt ccttcatcac gcgcaggttc agccgcatct
      601 cttctagaat ttcttcgcgt gaacgctcgg actgcttctc ctcgcggcga atcatcgctt
      661 cgtagatggg aatgccgttt tccaaacagt atttacggag ggattctccg ttttcaaacg
      721 cttccgataa caacatagtt ttccctcctt tacacggcaa acagcttgat aatgccgctg
      781 cagagtttca gtatcatggc ctgcatgtcg cgcgtaacag gctgatcggt ttctatggtc
      841 atatacgcgt cctcgccttt accgtggcgg aacacgcgca tgaacgcgat gttcacgctg
      901 agctgcgcca gcacatgaga aacttccgca atgacaccgg gcttatcggt atgctgaatg
      961 accagcgtcg gatactcgcc ggagaactcg accgcgaggc cgttgatctc cgtaatgcgg
     1021 atgtttccgc cgcccacgga ttcgccgaca acgtccgtcg tctgcccgtt tgagccggtg
     1081 atcacgatgc gcgcggtgtt gggaaaagcg ggttgctcct cggaaaactc catcgtgact
     1141 aacacgccca tttcgcgcgc aacgcggtaa gcgtccttga tgcgcgggtc gtccggctcc
     1201 atgccgagag cgcccgcaat cagcgcttta tccgtgccgt ggccgcgccc ggtttcggca
     1261 aacgagccgt agagcgtgaa cttgacttcg gctacatcgt cttccgcgat atgccgcgca
     1321 acgcgggaaa gcctcgcggc tcccgccgta tgcgagctgg aggggccggt cattcttgga
     1381 cctatgatat cgaatacaga aaaatcttcc atcatttcat ctccgaatcc ttctggcaga
     1441 taagatttgc aggactaaaa aagaccggcg caccgttttt ggcgatgcgc cgatcaaggt
     1501 tttgccgcgc acccgttctt tgacaaacac gtctccgggc gacgctccga caggttgctc
     1561 ctttcaggct gctccgcggc acatcgcatt atccgctgaa tgctgcttcc gtcaggacct
     1621 gacatggttc acggcctgcg attgcacggg acccgacgct caacgcccct tatcgggatc
     1681 tgaccccaca gacgcttgcc ggggaacggg atttccaccc tgctgtagcg gattgcaggt
     1741 acagggcacc gctagttccc cgcttagcgc ggcaaactga ttaacagaat tattgtaaca
     1801 tgctttatcg aacatgcaaa atgatttctt gcgtgtttga caaaagtttg cttttcgcat
     1861 caaacgaact gatcggcccg gaaattgcta ccgcagacgt ttgactgcgt atgccgcggc
     1921 ggcacgaacg ccctcgcgct gatcttccaa tagtttttct atgagcggaa gaaggtcttt
     1981 tctgcccagc tttgccgcca tgacgcaggc gtgctgaata agacgaccgt ttttgttgag
     2041 atttgtgccg acgatcttta gcgcgggttt gacgtcgccg gataaaagct tttccagcgc
     2101 gaacacttcc ggcacgtccg aaaccggttc gatttcgcgg ttgtacgggc agatcgactg
     2161 gcagagttcg cagccgagaa tgcttgtcat cgcgtccatg acccagtctt ccatcgggtc
     2221 gccgcccatg taagcccgcg cgcaggtttt gtagtcgtag ccgttatgag agatggcgcc
     2281 gctggggcag acgcgctcgc aggcatggca ttttgtgcag aacggttctt cctgcgtttg
     2341 aacaggcgta tacgaaactt ccggcagatc gatgagcagc gtctgcacgc tgtatcgcgt
     2401 tccgtagccg gggatatacg tcagtccgtt cttaagcggt acgccgacgc cgtttcgaat
     2461 tagcagttcg cgaaccggaa tatacgcgcg cttggcttga atgccctgct cgttgagcgc
     2521 gcgaatcagc ttgtttgatg catgatatgc cttgttgctg gaaggatagt tgccggaaac
     2581 gggaacagaa tccgggtaag gacagtatgg cagaatcagc gcgagaatgg cgtttgccca
     2641 ggaatagacg gcgtgaacgt ccgcggttaa actcgtcccg gaatgatgca gagcgccgtc
     2701 ttctaaacgg cgctcaaagt aggtaaacgg ttcggttgag aacacaaaag cttcggcaaa
     2761 accgaattcc tttgccattt cgcggatgtt catctgccct gttcctgcag catctctttg
     2821 gtcttcatca gcctagtgag attgccgcgc tcgttctctt ccagcttcat ctggatgcgc
     2881 cggatcgtct ccgtaaactg cggaatcata acgtattcca gcgcgttgac cctacggcgc
     2941 gtcttttcaa tttcgtctgc gagcctgccg caggcctttt caatttccgc aagctgaacc
     3001 agcagcggca gaacttctgc cagcagctgg acggccatat cgagatccgc gctggtggtt
     3061 gcaaagccgt atgggtacgt aaacccttcc tgcgcggaga ttgcgagctt cggcacctga
     3121 atggagagaa cgtgctcgat tcccgccgta acatccaacg cgcgcgcggg atagatcagc
     3181 gcttcctcaa tctccgcgcc gctcatggac gcgcgagcaa gcacgaagct cttcatggcg
     3241 cgggaaagct tttcttccac atccgcacgg agacgcttgt tctcacgggc atactcaatg
     3301 aaccgcctga cgagttcgtc ccgcttgtcc ttcatcatct tatgtccgcg aacggcaacc
     3361 tttttgcgtt tcttgaggtt gctcagttcc atgcgggtcg gttttacttg gatggcggcc
     3421 attggctacg cctccttggg atagtatttc tcgatgtaag cgtcacgaat acgcttgagt
     3481 tcgctcttgg gcaggatgcc gagaagcttc cagccaaggt caagcgtctc ttcgatggtg
     3541 cggtttgtgt agtacccctg ggacacatac tgctcttcga acgcgtcggc aaactttgcg
     3601 tagagcttgt ccgtttcgga aagcgccgcg tcgccaagga tgacggcgag ttcctttgcg
     3661 tccttgccgc gcgaatacgc cgagaagagc tggttcatgg tgtccgcgtg gtcttcgcgc
     3721 gttttgccct tgccgatgcc cttatctttc aaacgcgaca gcgacggaag aacgttgatg
     3781 ggcggggtta agcctttgcg gtagagttcg cgtgaaagga tgatctgccc ttccgtgatg
     3841 tatccggtca agtcgggaat cgggtgggtc acgtcgtctt ccggcatgga gagaatcgga
     3901 atcatcgtga tggaacccgc gttttcacgg attctgccag cgcgctcata catggtcgca
     3961 aggtcggtat agaggtagcc cggatagcct ctgcggccgg gaacttcctt acgggctgcg
     4021 gaaacttcgc gcagcgcttc ggcatagttc gtaatatccg taatgatgac cagcacgtgc
     4081 atgcccagct cgtacgccag atactccgcg gcggtaatcg ccatacgcgg ggtcgcgatg
     4141 cgctcgatag ccgggtcgtt tgcgaggttg atgaacgtaa ccgtcttttc aattgcgccg
     4201 gtttttctaa agtcgctgat aaagaagtcc gcttcctcaa acgtaatgcc gacggcggca
     4261 aacacaaccg cgaacttctc atccgaaccc agcacctttg cctgacgcgc aatctgcgcg
     4321 gcaaggttgg catgcggcag accggagccg gagaacaccg gcagtttctg gccgcgaacc
     4381 agcgtattga gcccgtcgat ggcggaaatg cccgtttgaa tgaactcgct cgggtagtcg
     4441 cgcgcagtcg gattcatagg cgtgccgttg at
//
LOCUS       DAOMGY010000035         4471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_20037_length_4471_cov_3.413043, whole genome shotgun sequence.
ACCESSION   DAOMGY010000035 DAOMGY010000000
VERSION     DAOMGY010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20037_length_4471_cov_3.413043"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLR57_01045"
     CDS             complement(<1..326)
                     /locus_tag="PLR57_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase"
                     /protein_id="HQQ40069.1"
                     /translation="MNQKTIAVLFGGCSPEYGVSLASAYSVLCHMDLQKYKPFPVGIT
                     PEGDWFAFGGEFEKISSDQWHNSADCTPAAFNPGRTGRSLLLLRPDGVQPVLIDAAFP
                     VLHGRNG"
     gene            complement(323..1156)
                     /gene="vanW"
                     /locus_tag="PLR57_01050"
     CDS             complement(323..1156)
                     /gene="vanW"
                     /locus_tag="PLR57_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein VanW"
                     /protein_id="HQQ40070.1"
                     /translation="MNIKNVLRQSPLLQRIRKRQRIFCFYAGMQLDGSRYCREQSKAF
                     YPHLLFETRCPLYNRATGFGMTYQENKVYNLKLAAAVLNGVVIRPGETFSFWRLARRA
                     DRRTPYRDGLVVIGGKLTTAPGGGLCQMSNSLFWMFLNSPLTIIERYGHAVKDFPEPP
                     SDAPIGVDATISEGWLDLKVKNDTNAVYQIGISFDEAFIITSIYTGRDDGLLYRAVSG
                     TPRYLRENGKVFEEVDVLRRTIHARTGAQINECRLYRNRCEIGYPLPEGTQIVQKGDP
                     V"
     gene            complement(1287..2411)
                     /locus_tag="PLR57_01055"
     CDS             complement(1287..2411)
                     /locus_tag="PLR57_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012620025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQQ40071.1"
                     /translation="MKSRSLRGKVYQKLLWKLFFQTVVVLGASFLLTLGTYAAVRGRL
                     ADFLVNAISYVLSLNWDSARDVYQQVIRNNITLAFGATFIVYFLILMRILMPWFTRFF
                     DRIVSGIDHLAEESDEPIRMGDELKFMEVTLNEVKQKLKQRTEAAKLEEQRKNDLVLY
                     LAHDIRTPLTSVIGYLSLLDEAPDMPAEQRAKYTRITLEKANRLEKLINEFFEITRYN
                     FQQGKLEKEPIDLYYMLVQMVDEFYPILNERGNTAVLDADETLTVGGDSAKLARVFNN
                     ILNNAAVYSDPGTPIVISAKKAGASVVITFQNQGPTIPADKLSVIFEKFFRLDEARVS
                     DTGGSGLGLSIAKEIVTLHGGTIQAQSKERQTTFTVLLPA"
     gene            complement(2454..3155)
                     /gene="vanR"
                     /locus_tag="PLR57_01060"
     CDS             complement(2454..3155)
                     /gene="vanR"
                     /locus_tag="PLR57_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HQQ40072.1"
                     /translation="MSEKILVVDDEHEIADLVEVYLKAENFNVYKFYSATEALACIET
                     IEMDLAILDVMLPDISGFALCRRIREQYAYPVIMLTAKDDESDKINGLTLGADDYITK
                     PFRPLEMLARVKAQLRRYKKYNSTQAASPHTAVLVHGGLTMDISNHACMLNERPLELT
                     PTEFAILRILLEKKGSVVSAEELFHEIWQDEYYSKSNNTITVHIRHLREKMGDSAENP
                     SHIRTVWGIGYKIEQ"
     gene            3562..4236
                     /locus_tag="PLR57_01065"
     CDS             3562..4236
                     /locus_tag="PLR57_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQQ40073.1"
                     /translation="MRLLLVEDDADLAFALKQCLIQEGYDCDACPNGADAAYYLQNGG
                     YGAVVLDRMLPEKDGLTILREMRSAGNETPVLMLTALDTVKDRTDGLDAGADDYLVKP
                     FAMPELLSRIRALIRRPPMLREDSLLRFADLVLDARQCKLSCKQSEQTLSRKETAVLE
                     LFFEHPEHVLSRTALLSRGWGGDETVEDGNVDNYIYFLRRRLKAAGTRAGIRTVHGVG
                     YQLTLE"
     gene            4266..>4471
                     /locus_tag="PLR57_01070"
     CDS             4266..>4471
                     /locus_tag="PLR57_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40074.1"
                     /translation="MEERKLRSHLIRIYVVTVACILAALLTVVLLLSVREIELKSRES
                     FSTLLTAIADDLQVSNVVRHSAFRR"
BASE COUNT          973 a         1155 c         1249 g         1094 t
ORIGIN      
        1 ccgtttcttc catgcagaac ggggaatgcg gcgtcgatta agacaggctg aacgccgtct
       61 gggcgcagca gcagcaagga acgacccgtt ctgcccgggt tgaacgcagc gggtgtgcag
      121 tccgcgctgt tatgccattg atcggaggat attttttcga actctcctcc aaaagcgaac
      181 cagtcccctt ccggcgtgat gccgacggga aacggtttat acttttgtaa gtccatatgg
      241 caaagcacgg agtatgccga agcaagagat acgccgtact ccggcgaaca gccgccaaat
      301 aggacggcga tggttttctg attcataccg ggtctccttt ttgtacaatt tgcgtgcctt
      361 cagggagcgg atagccgatt tcgcagcggt tgcggtagag ccggcactcg tttatttggg
      421 cacccgtccg cgcatggatc gtccttcgga gcacgtcaac ttcctcaaag actttaccgt
      481 tttcgcgcag gtagcgcggg gttccgctga ccgcgcgata gagcaggccg tcgtctctgc
      541 ccgtatagat gctcgtaatg ataaatgcct cgtcgaagga aatgccgatc tggtaaacgg
      601 cattcgtatc atttttaacc ttcaggtcga gccatccttc cgagatggtg gcgtccacgc
      661 caattggcgc gtcgctcggc ggttccggga agtccttcac ggcgtggccg tagcgctcga
      721 taatggtcag aggggagttg aggaacatcc agaacagcga attgctcatc tggcagagcc
      781 ctccgcccgg cgcggtcgta agcttgccgc cgatgacgac aagcccgtcc cgatatggcg
      841 ttcgacggtc ggcgcggcgc gcgagacgcc aaaaggagaa ggtttctcct ggccggatga
      901 cgacaccgtt caaaaccgcg gctgcgagct tgaggttgta aaccttattc tcctgatacg
      961 tcatgccgaa tccggttgcg cggttataca gcgggcatcg cgtttcaaag agcaggtgcg
     1021 ggtaaaacgc tttggattgc tcccggcagt agcggctgcc gtccagctgc attcccgcgt
     1081 aaaagcagaa aatccgctgc cgctttcgaa ttcgctgcaa tagcggcgat tgtctgagaa
     1141 cgttttttat attcattgaa tgcctttccc aataaaaaaa ctgcctgtcc ggtcgacagc
     1201 aaaatgctat caaaaccgga caggccgttc gttaagtttg gacggatgaa gtcttaagat
     1261 tttcttaatg cacggtggtt tttctattaa gcgggcagca atacggtaaa agtggtttgc
     1321 cgttccttac tctgcgcctg aatcgttccg ccgtggaggg taacgatctc ctttgctatg
     1381 gataatccaa gtcccgatcc gccggtatcg gaaacacgcg cttcatcgag gcggaagaac
     1441 ttttcaaaga taacggaaag cttgtccgct gggatcgtag ggccttgatt ctgaaacgta
     1501 ataacaaccg atgcgccagc cttttttgcg gagatcacaa tcggcgtgcc ggggtcgctg
     1561 tataccgcgg cgttattgag gatattgttg aatacgcgcg cgagcttggc ggaatcgccg
     1621 ccgacggtga gggtttcgtc cgcatccagt accgccgtgt tgccgcgttc gttgagaatc
     1681 gggtagaact cgtccaccat ctgcacgagc atataataca gatcgatagg ctctttttcc
     1741 agcttgccct gctgaaaatt atatctcgta atttcaaaga attcgttgat gagcttttcg
     1801 agacggtttg ctttttcgag cgtgatacgc gtatatttcg ccctctgttc agcgggcata
     1861 tcgggcgctt cgtcgagcag gctgagatag ccgatcacgg aagtcagcgg ggtgcggata
     1921 tcgtgcgcca gatacaagac aaggtcgttc tttctctgtt cctcaagttt ggccgcctcg
     1981 gtgcgttgtt tcagcttttg tttgacttcg ttgagcgtga cttccatgaa tttcagttcg
     2041 tctcccatgc gaatgggctc atccgattcc tccgccagat gatctatacc ggaaacgata
     2101 cgatcaaaga agcgcgtaaa ccatggcatc aggatacgca taaggatcag gaaataaaca
     2161 atgaatgttg cgccaaaggc cagcgtaata ttattccgga tcacttgctg ataaacatcc
     2221 cgcgccgagt cccagttcag actcaatacg taactgattg cgttgaccag aaaatccgca
     2281 agccgccctc tcacagcggc gtacgtgcca agcgtaagta aaaaagaagc gcccagcacg
     2341 acgactgttt gaaagaacag cttccagagc agtttttgat aaacttttcc tcgtaatgat
     2401 cgactcttca attcttctgc cgcctattca taagttcctg ataaaagaat gaactattgc
     2461 tcaattttgt atccaattcc ccagaccgtc cggatgtggc tcgggttttc tgccgaatct
     2521 cccattttct cgcgaaggtg gcggatatgg acggtgatcg tattattgct tttactgtag
     2581 tattcgtcct gccagatctc gtgaaagagc tcctcggcgc tcacgacgct gccttttttc
     2641 tccagcagga tgcggagtat ggcaaactcg gtcggcgtca gctccagcgg acgctcgttg
     2701 agcatacagg cgtggttgga gatatccatg gtcagcccgc cgtggacgag caccgctgtg
     2761 tgcggggaag ctgcctgcgt ggaattgtat ttcttatacc ggcgcagctg tgctttgacg
     2821 cgcgcgagca tttcgagcgg gcggaacggc tttgtgatgt agtcgtccgc gccgagggtc
     2881 aggccgttga tcttgtcgga ttcatcgtcc ttggcggtca gcatgatcac gggataggcg
     2941 tactgctcgc gtattctgcg gcagagcgca aacccgctga tatccggcag catgacgtcc
     3001 agaatcgcca gatccatctc aatggtttcg atgcaggcga gcgcttccgt tgcggaatag
     3061 aacttgtaaa cgttgaagtt ttcggctttc aggtatactt ccaccagatc cgcaatttcg
     3121 tgttcgtcgt cgacgaccag gatcttttcg ctcatcgttt cgtttttctc ctgcttctcc
     3181 tgaggataat acctagggta tcgtaccaga ccggacacac ctaagtcttt gcaatctatt
     3241 aagagattct taagattttg cttttcgcct tcataaacga gccgaatagt ggcttatcaa
     3301 ggcattttcg ggcgcacttt ttttggagat tggttttgcc tgtcccgatt gtagtatgaa
     3361 aacccgttcc ggtcaatgac ccgtactgtt gcggaatccg gctgaatcag cacgttttta
     3421 acaagccgaa agcgccgctg cggaagagcg gtcgggtgcg gtcaaagcca cggcgggtaa
     3481 gtccgggttg acgtgcgcag aataccatac tacaatcgtg gcaggttgca gacagaataa
     3541 ggactgctcg ggagggaaca tatgcgcctg ttgctggtgg aggacgatgc ggacctcgcg
     3601 tttgcgctga agcaatgcct cattcaggag ggatacgact gcgacgcttg cccaaatggt
     3661 gcagacgccg cttattacct gcaaaatggc ggatatggcg cggtggtgct ggaccgcatg
     3721 ctgccggaaa aagacggact taccattctg cgggagatgc gttcagcagg aaacgaaacc
     3781 cccgtgctca tgcttactgc gctggatact gtaaaggacc ggacggacgg gttggatgcg
     3841 ggcgcggacg attacctcgt caagccgttt gccatgccgg aactgctctc gcgcatccgc
     3901 gcgctgattc gccgcccgcc gatgctccgc gaagacagct tgctccgctt tgcggatctg
     3961 gttttggacg cgcgccaatg caaactatcc tgcaaacaat ccgagcagac gctctcgcgt
     4021 aaggagacgg cggtgctgga actgttcttt gagcatccgg agcacgtatt gtcccgtacg
     4081 gcgctgctct cgcgcgggtg gggtggggac gaaacggtag aggacggaaa cgttgacaac
     4141 tatatctact ttctccggcg caggctcaag gccgccggaa cgcgcgctgg cattagaacg
     4201 gtacatggcg taggctacca actgacgctc gaataaacaa gcttttatcg gaggcgcatc
     4261 ggaacttgga agagcggaaa ctgcgttcgc atttgatacg aatttacgtc gtcacggtcg
     4321 cttgcattct cgcggcgctg ctgacagtgg ttttgctgct ctcggttcgg gaaattgagc
     4381 tgaaaagccg tgaaagtttt tctaccctgc tgacggcgat tgcggacgat ctgcaggtaa
     4441 gtaacgttgt gcgccacagc gcatttagac g
//
LOCUS       DAOMGY010000036         4468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_20053_length_4468_cov_3.184002, whole genome shotgun sequence.
ACCESSION   DAOMGY010000036 DAOMGY010000000
VERSION     DAOMGY010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20053_length_4468_cov_3.184002"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLR57_01075"
     CDS             complement(<1..337)
                     /locus_tag="PLR57_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40075.1"
                     /translation="MDSELKVSTKKAIGRRPDLSQQPSVFPVLTEEPSEAAKKQSAAA
                     RGSAKWVRVFTWIGTVLVWLPLALSIVYYVYSAVLKTPYALFYAFVVFTALRIPILIG
                     SVFLFASAAT"
     gene            complement(412..867)
                     /locus_tag="PLR57_01080"
     CDS             complement(412..867)
                     /locus_tag="PLR57_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40076.1"
                     /translation="MKTKAVLAKVFAIAGAVLVWAPILFMLLTGVVGSISSKMLLMDY
                     LLLAELFFVVVAGLVLLFVSSLLGRALVKWIGFTAAAGVIALALSMLLAQTTGLASGA
                     IAAEGWPLILVMGLLILYNVLVLAVGILGILLIKALFRKKPAPAEEPAV"
     gene            1157..1672
                     /locus_tag="PLR57_01085"
     CDS             1157..1672
                     /locus_tag="PLR57_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40077.1"
                     /translation="MTAIDWETMWSIRDQLVEELKNRGLLPERSLYIRREDGGVFALA
                     VRTDAPRVLSFDWNGTSCRYRALENPHIALSEYDVQPGGFGGMFGLGEKGAHGWMLRV
                     LEETSLVWETPILPGMTAIADLFFREDLILNGRRKKGTVPHWQLMPEDEQRCEEIIAV
                     WERLLAGVNAR"
     gene            1859..2362
                     /locus_tag="PLR57_01090"
     CDS             1859..2362
                     /locus_tag="PLR57_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ40078.1"
                     /translation="MERKEKHALQKAVRTADEFAASVKQIERLLFAASYAILGNSDAC
                     ADAVQAALMKAWESRSSLRSGELFKSWMVRIVHNESRNLLRKKPNLPLDEDIPATEED
                     QARLDVKLALSRLPEQTRLIVMLYYFERYPVKEICALLELPEGTVHSRLSRAREQLRK
                     ELTDYER"
     gene            2352..3806
                     /locus_tag="PLR57_01095"
     CDS             2352..3806
                     /locus_tag="PLR57_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40079.1"
                     /translation="MNGNEWTLEAFLYENRISPAPEFSAAIDQTCNQIRRREAKATRI
                     EAGARAKKRRYQRSLRWLVSAAAVIVVLVTLLSIPSVSQAMRSWFGDLFRLTDYMAAE
                     PENREENADIAGAVQTPVPAQTSYSLRYLDETEYIDGVDEWRLSNGFAAFNREDYAWV
                     ADLRPEAKEILYDGRNLIINTQINASPARFLGTYGGEGERFDLWTNSVSVTVNGQPFT
                     DFTDQGGGLVLKSFQNADGSGYDLAKVSAADSVLEQTTLIGNNSPAFPSGPVTVTIEM
                     WLVDGEIDDMATVGLVAVLTQTVTFDATEGNANLSSAVSATQQLCGVAPLTIDGDGTL
                     ENCMFDYAAVTVSASIERRATGVSVSIHYSFPTEEEKMRLWKSIVLGVVGHSGIQYEA
                     IVDGQSIGKVWHAGNYLGTWDDPVLEVPLTESELASVQSITLCPIVRYISSYTSGGAE
                     YTDMPFDETLTLAQYGARYTEVPLEGCDIVIPLN"
     gene            complement(3951..>4468)
                     /locus_tag="PLR57_01100"
     CDS             complement(3951..>4468)
                     /locus_tag="PLR57_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40080.1"
                     /translation="MLCRTFMKYDDFYRYYVPLGMNERFFLGAEEDDFLLDGFCIRLF
                     RDVKKAESKNDICLDIIKKEGLLDLLTTPEIDLSSWQTIFSSLMQRQKNIIVEGERAD
                     PAAPQFAIGRIERVTAKRLYLRHFDGDGVWQEEPYEISYSEITSVTFGSRYVDVFSKY
                     LPPLPENFGQI"
BASE COUNT         1076 a         1157 c         1305 g          930 t
ORIGIN      
        1 tcgtagcggc ggaggcaaac agaaaaacgg atccaatcag gattggaatg cgtagcgcag
       61 tgaatacgac aaatgcgtag aacagagcgt agggggtctt caaaacagcg gagtatacat
      121 agtacacaat tgagagcgcc agaggaagcc aaacgagcac ggtaccaatc caagtaaaaa
      181 cgcgcaccca cttggcgctg ccgcgcgcgg cggcagactg ctttttcgcc gcctcggagg
      241 gctcttccgt cagaactggg aaaacgctgg gctgctggga caggtcgggg cgacgcccga
      301 ttgctttttt cgtggagact ttcagttcgg aatccatgtg cgcaactcaa tacccggcag
      361 ggctgaccct gccgatcctt tccagaaaat agggatgctg atttgagaat actaaaccgc
      421 gggttcctcc gcgggagcgg gctttttacg gaacagggct ttgattaaga ggatgccgag
      481 gatgccgacg gcaagcacca gcacgttgta gagaatgagc agacccatca cgaggatgag
      541 cggccagcct tccgcggcaa tggcgcccga ggcaagaccc gtcgtctgcg caagcagcat
      601 ggaaagcgcc agcgcgatca cgccggctgc ggctgtaaag ccgatccact ttacgagcgc
      661 acgccccagc aggctcgaaa caaacaatag tacgagcccg gcaacgacga cgaagaacag
      721 ctctgccagc agcaggtaat ccatcagtag catctttgag gaaatggaac cgacaacgcc
      781 ggtgagcagc ataaagagaa tcggcgccca aaccagaacg gctccggcga tggcgaatac
      841 tttcgcgagt acggctttcg ttttcataaa gcaaacctcc agttcgcatg tcggcagggt
      901 aaggaaagga gggggacgtt tacgtctttg aaatctctaa ctattatact caatattcca
      961 gtgaattacc actggccatc aaaaaacgcg ggaagtttcc gtccttcttc aaaaaacagc
     1021 gcaattttcg acaagttacg ataccgagca cgcgttcgac ggcggatttc gttgagttcc
     1081 ggcattacct aacatataat acagggtgaa tcggtacgga attgttttac agggcgaaga
     1141 aaggcggctg attttcttga cggcgattga ttgggaaacc atgtggagca tccgcgacca
     1201 actggttgag gagctcaaaa acagaggtct tctgccggag aggtcgctct acattcgccg
     1261 cgaggacggc ggcgtgttcg cgctcgcggt tcgaacagac gcgccgcgcg ttctttcgtt
     1321 cgactggaac ggcacttcct gccgctaccg cgcgcttgaa aatccacaca tcgcgctctc
     1381 cgaatacgac gtccagcccg gcggattcgg cggcatgttc ggcctcggcg aaaaaggcgc
     1441 gcatggctgg atgctccgcg tactcgaaga aacatcactc gtatgggaaa cccctattct
     1501 gcctggtatg acggcgattg ccgatttgtt ttttcgggaa gacctcattt taaacggcag
     1561 acgcaaaaaa ggcaccgttc cgcactggca gctgatgccg gaggacgaac aacggtgcga
     1621 ggagatcatc gcggtttggg agcggctgct cgcgggagta aacgcgcgat aaaagaaccg
     1681 actatcgcta aatcaactat tccaccattt tgagatgcct ctgccgatga cgcgtctttg
     1741 ccgccgggag catttgctgc ggcggctttt ttctaatctt atgccgtttt gaaaacaaat
     1801 ggaagatttc gccggcttgc tgtgtcatat agatagacac acagcaaaag gagggcaaat
     1861 ggaacgaaaa gaaaagcatg cgctgcaaaa agccgtccgt acggcggatg agtttgccgc
     1921 ttcggtcaag cagattgagc ggctcttgtt tgcggcgagc tacgccatac tgggcaacag
     1981 cgacgcctgc gcggacgcgg tgcaggccgc gctcatgaaa gcctgggaga gccggagcag
     2041 ccttcgcagc ggcgaactgt ttaaaagctg gatggtgcgg attgttcata acgaaagcag
     2101 gaatcttctg cgcaaaaagc caaaccttcc gctggacgag gacattcccg caacggaaga
     2161 agatcaggcc aggctggacg taaagctcgc gttgtctcgt ctgccggagc aaacgcggct
     2221 tatcgtgatg ctgtattact ttgagcgata cccggtcaag gagatttgcg cgcttctgga
     2281 actgccggag ggcacggtac attcgcggct ttcccgcgcc agagaacaac tcagaaagga
     2341 gctcacggac tatgaacggt aatgaatgga cgctggaagc gtttttatat gaaaaccgca
     2401 tttctcctgc gccggaattc tccgcggcaa tcgatcaaac ctgcaatcaa attcggcgtc
     2461 gcgaggccaa agcaacgcgc atagaagcag gcgcaagagc aaaaaaacgc cgttatcagc
     2521 ggtcgctgcg gtggctcgtt tcggcggcgg cggtcatcgt cgtcctcgtg acgctgcttt
     2581 cgattcccag cgtgtcgcag gcgatgcgga gctggtttgg cgatttgttc cggctcacgg
     2641 actatatggc ggcggagcct gagaatcgcg aggagaacgc cgacatcgct ggggcggttc
     2701 aaacgcccgt gcctgcacaa acgagttatt ccctgcggta tctggacgaa acggaatata
     2761 tagacggggt cgacgaatgg cgattgagca atggatttgc cgcgttcaac cgggaggatt
     2821 acgcctgggt ggccgacttg cggccggagg cgaaagagat actttacgac gggcggaatc
     2881 tgattatcaa cacgcagatt aacgcaagcc ccgcgcgctt tctcggcact tacggcgggg
     2941 aaggcgagcg gtttgaccta tggacaaaca gcgtttccgt aacggtgaac ggtcagccgt
     3001 ttacagattt taccgatcag ggcggcggac ttgtgctcaa gtcttttcaa aacgcggacg
     3061 gatcaggcta cgatttggca aaagtgagcg ccgcggacag cgttctggag cagacgactc
     3121 tgattggcaa taactcgccc gcgttccctt ctgggccggt gacggttaca attgaaatgt
     3181 ggctcgtgga cggtgaaatc gacgacatgg cgacggtcgg gctggttgca gtcctcacgc
     3241 agaccgtaac ctttgacgcc acggaaggta acgcaaatct ctcgtcagcg gtttcggcga
     3301 cgcagcaact ctgcggggtt gcgccgctca caatcgacgg tgacggcacg ctggagaact
     3361 gcatgttcga ctacgcagcc gttacggtga gcgcaagcat tgagcgccgg gcgacgggcg
     3421 tgagcgttag tattcactat tccttcccga cggaagagga aaaaatgcgg ctgtggaagt
     3481 cgattgtgct tggcgttgta gggcatagcg gaatccagta cgaagcgatt gtcgacgggc
     3541 agagcatcgg caaggtttgg cacgcgggta attatcttgg cacgtgggac gatcccgtgc
     3601 tggaagttcc cctcacggaa tcggaacttg ccagcgtgca gagcatcacg ctgtgtccga
     3661 tcgtgcgcta tatctcttcc tacacgtccg gcggagctga atatacggac atgccctttg
     3721 acgaaacatt gacgcttgcg cagtatggtg cgcggtacac agaggttccg ctggaaggct
     3781 gcgatatcgt gattccgctg aactgagggc gggctaacag acaggcctta aaggagcgaa
     3841 acaataaaac ggggtatgcg ctgaatcgca caccccgttt ttcgttgcat tgaatcactt
     3901 tttgctcgtg ggaataccgt ccaaactctt agttattttt tagtaacgat ttagatttga
     3961 ccgaaattct ccggcagcgg gggaaggtat ttcgaaaaca catcaacata tcgggaaccg
     4021 aacgtcacgc tcgtgatttc cgaatacgaa atttcgtacg gctcctcctg ccagacgccg
     4081 tcgccatcga aatgccgcag gtacagacgc tttgctgtga cgcgctcaat tcgtccgatg
     4141 gcgaactgcg gcgcggcagg gtcggcgcgt tcgccttcca cgatgatgtt tttctgccgc
     4201 tgcatcaggc tcgaaaagat cgtctgccaa ctggaaaggt cgatctccgg cgtggtaaga
     4261 aggtctagaa ggccttcttt ttttatgatg tcgaggcaga tatcgttctt actttccgct
     4321 ttttttacgt cccgaaacag ccggatgcag aaaccgtcca gcagaaaatc gtcttcctcc
     4381 gcgccgagaa agaaccgctc gttcatgccg agcggaacgt agtagcgata gaagtcgtcg
     4441 tacttcataa acgttctgca gagcattc
//
LOCUS       DAOMGY010000037         4446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_20200_length_4446_cov_3.019813, whole genome shotgun sequence.
ACCESSION   DAOMGY010000037 DAOMGY010000000
VERSION     DAOMGY010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20200_length_4446_cov_3.019813"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /gene="feoB"
                     /locus_tag="PLR57_01105"
     CDS             <1..1576
                     /gene="feoB"
                     /locus_tag="PLR57_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HQQ40081.1"
                     /translation="NEGVSELVNQALAVAKKRIVPAVQDFCPDGPVHRCIHAVAHVIE
                     DHARNIGVSKRFCATKLIEGDVSFAERLDLDQNELDLIEHSVLEMENETGFDRNEALA
                     DMRYTFIEGIVSAAVVKCCESREHKRSVGIDKILTGKYTALPVFFGVMFLVFWLTFNV
                     IGSTLADWLTAGIGWLTGVVDAGLTAYGINPVVKSLVVDGIFAGVGSVLSFLPIVVTL
                     FFFLSILEDTGYMARIAFVMDRLLRKIGLSGRSFVPMLIGFGCSVPAIMATRTLASDR
                     DRKMTILLTPFMSCSAKIPIYAVFCAAFFARYQALVMIGLYAAGIILGILVALLLKGT
                     AFRGQPVPFVMELPNYRMPSPKNVLLLLWEKAKDFLERAFTIILLASIVIWFLQTFDL
                     RLNVVTESASSLLALVGRLIAPIFAPLGFADWRVATSLISGFVAKEAVVSTLSVLTGT
                     GITQLHTVLSSMFTPLSAVSFLLFTLLYTPCVAAVATMKRELKSGIATLGVVIMQCGI
                     AWIVAFAAYQIGRLLL"
     gene            1962..2288
                     /locus_tag="PLR57_01110"
     CDS             1962..2288
                     /locus_tag="PLR57_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQQ40082.1"
                     /translation="MESSYAEQAKMMKAFCDENRLQILELLRGGEKCACMLLEHLPIG
                     QSALSYHMKILTESGVVASRQEGKWTHYRLSEAGCEKARALLEGITTPSEERSDCVCD
                     TTGEPA"
     gene            2320..3333
                     /locus_tag="PLR57_01115"
     CDS             2320..3333
                     /locus_tag="PLR57_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HQQ40083.1"
                     /translation="MQILKTIWDFFQNQILAMKWLNELIGGLLGALGLDTANRWVGSL
                     QFFAYDVIKITILLCTLIFVISYIQSFFPPERSKKILGRFHGLGANTVAALLGTVTPF
                     CSCSSIPLFIGFTSAGLPVGVTFSFLISSPMVDLGSLVLLMSIFGAKVAIIYVLFGLI
                     IAVLGGTLIEKLKMERYVESFILTAGSVDIESPDLTIKDRLIYAKEQMVSTFKKVFPY
                     VLIGVGIGAVIHNWIPEEWIVAVLGNKNPFGVVLATLIGVPMYADIFGTIPIAEALFS
                     KGALLGSVLAFMMAVTTLSLPSMIMLRKAVKPQLLALFIAICTVGIILIGYLFNALQP
                     LIA"
     gene            3342..3623
                     /locus_tag="PLR57_01120"
     CDS             3342..3623
                     /locus_tag="PLR57_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQQ40084.1"
                     /translation="MAKSWYPVIDYSSCTECGTCSSKCPHQVYDMKKAPVPVVLNPAS
                     CIDHCHGCGNRCPVGAITYVGDDTGWTPPHGEIRQDEPACGCGGGSCCG"
     gene            3616..4164
                     /locus_tag="PLR57_01125"
     CDS             3616..4164
                     /locus_tag="PLR57_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQQ40085.1"
                     /translation="MAEAKPEKKKSPLKKTLLVIAIIAAIAVIWFVKNGGTQQDTTAS
                     TDLTQEDFALEAESIDLNALMAYGLPVIIDFGSDSCIPCKEMAPILAAMNEAFQGKAI
                     IKFVDVWKNADGANGFPVQVIPTQILFDAAGNPYIPSKDLGIEFIVYSDKTTNEHLFT
                     AHQGGLTEEQMRAILTDMGVTE"
     gene            4161..>4446
                     /locus_tag="PLR57_01130"
     CDS             4161..>4446
                     /locus_tag="PLR57_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HQQ40086.1"
                     /translation="MIDLWLEQIGRVIGQNAWLAPLLALLAGVLTSFTPCALSSIPLV
                     VAYVGGIGVEPKKAFRLSLVFAFGAAITFTMLGVVAASVGQLLGTGTKWWY"
BASE COUNT          914 a         1153 c         1324 g         1055 t
ORIGIN      
        1 gaacgaaggc gtttcggaac tggtgaatca ggcgctggct gtggcaaaaa aacggatcgt
       61 ccctgccgtt caggacttct gcccggacgg ccccgtgcac cgctgcatcc acgccgttgc
      121 gcatgtcata gaagaccatg cgcgcaacat cggcgtttcc aaacggttct gcgccaccaa
      181 gctcatagaa ggcgacgttt cctttgccga acggctggat ctggatcaga acgagctgga
      241 tttaattgag cacagcgtgc tcgaaatgga aaacgaaacc gggttcgacc gcaacgaagc
      301 gctcgcggac atgcggtata cgtttattga aggcatcgtt tccgcggctg ttgtcaagtg
      361 ctgcgagagc cgcgagcaca agcgcagcgt cggcatcgac aagattctga cgggcaaata
      421 caccgcgctt ccggtctttt tcggggtgat gttcctcgtg ttctggctga cgttcaacgt
      481 tatcggaagc actttggcgg actggctcac ggcgggtatc ggctggctaa cgggcgtcgt
      541 ggacgcgggg ctgaccgcct acggcatcaa ccctgtggtg aaaagcttag ttgtggacgg
      601 catctttgcg ggcgtcggga gcgttttgag ctttctgccg atcgttgtga cgctgttctt
      661 cttcctctct attttggaag acacggggta tatggcgcgc atcgcgtttg taatggaccg
      721 cctgctgcgc aaaatcggcc tttccggcag gagctttgta cccatgctga tcgggtttgg
      781 ctgctccgtg cccgccatta tggcaacgcg gacgctcgct tccgaccgcg accgtaagat
      841 gacgattctt ttgacgccct ttatgagctg ttcggcaaag attccgatct atgcggtgtt
      901 ctgcgcggcg ttctttgcca gatatcaggc gctggtcatg attggcctct atgccgcggg
      961 catcatcctc ggcattctcg tggcgctcct gctcaagggc acggcgtttc gcggccagcc
     1021 ggttccgttt gtgatggagc tgcccaacta ccgcatgccg tcgccaaaga acgtgctctt
     1081 gctcctgtgg gaaaaagcga aagattttct ggagcgcgcg tttacgatta tcctcttggc
     1141 cagcatcgta atctggtttt tgcagacgtt cgacctgcgc ctcaacgttg tgacggaaag
     1201 cgcctccagc ctgctggcat tggtgggacg gctgatcgct ccgattttcg cgccgctggg
     1261 ctttgcggac tggcgcgttg caacgtcgct gatttccggc tttgttgcca aggaggcggt
     1321 ggtcagtacg ttgtccgtgc tcaccggcac cggaattacg cagctgcata cggtgctttc
     1381 cagcatgttt acgccgcttt cggcggtgag tttcctgctc tttacactgc tgtatacgcc
     1441 gtgcgtcgcc gcggtggcga caatgaagcg tgagctcaag agcggaattg cgaccctggg
     1501 cgtagtgatt atgcagtgcg gaattgcgtg gattgttgcg ttcgcggcct atcagatagg
     1561 acgtttgctg ttatgagcgt ttgggattat ttcgttttag cgctggttgc gctgattgcc
     1621 gggctttcgg tctggcgcat ggcaaaacgg cgcaagcagg gcggcggctg ttgttcgtcc
     1681 tgtgccggag ggtgcgccgg atgtgtccgc gccggcagtt gtgaagacac gaaaacaaaa
     1741 tagaaagaat tcatgatgaa gcatccccgc gcagcagcgg ggatgttttt tacgcggttt
     1801 ctctatatag aaaatcatat tgacaaattt cgatatgagc cgtatgatgg acatatcgaa
     1861 aaatatcgat atgaaaacgt cgagagaaca tcgttcaaaa aaggcaagtt caggccgccg
     1921 tttttaatcg aatcacaaca cgaaattacg aggcgtgagc aatggaatct tcctatgccg
     1981 aacaggcaaa aatgatgaaa gcgttttgtg acgaaaaccg cctgcagata cttgaacttc
     2041 tgcgcggcgg ggaaaagtgc gcctgtatgc tgctggagca cctgccgatc gggcaaagcg
     2101 cgctctccta ccatatgaaa atcctcacgg agtccggcgt tgtggcaagc aggcaggagg
     2161 gaaaatggac gcactaccgc ctgagcgaag cgggctgtga gaaagcgcgc gcgctgcttg
     2221 agggaattac aacgccttcc gaagaacgca gcgactgcgt ttgcgacaca accggggagc
     2281 ccgcgtaaca ggcagctcac taaatagtaa ggaacgatta tgcagatact gaaaacgatt
     2341 tgggacttct ttcaaaacca gatacttgcc atgaagtggc tgaacgaact gatcggcgga
     2401 cttttgggcg cgctcggcct cgacacggcg aaccgctggg tcggtagcct gcagttcttc
     2461 gcgtacgacg tgattaaaat tacgattttg ctctgcacgc tgatctttgt aatcagctac
     2521 atccagagct tttttccgcc ggagcgcagc aaaaaaatac tcgggcgctt tcacggcctc
     2581 ggcgcaaaca ccgtcgcggc gctgctcggc acggtaacgc cgttttgctc ctgctcgtcg
     2641 attccgctgt tcatcggctt tacctcggcg gggctcccgg tcggcgttac gttttcgttt
     2701 ctcatttcgt cgcccatggt cgatctgggt tcgctcgtgc tgctcatgag catcttcggc
     2761 gcgaaggtcg cgattatcta cgtgctcttc gggctgatta tcgcggtgct tggcggcacg
     2821 ctgattgaaa agctgaagat ggagcggtat gtcgagagct ttattctcac cgcgggcagc
     2881 gtggatatcg agtcgcccga cctgacaatc aaggatcggc tgatctacgc aaaagagcag
     2941 atggtgagta cgttcaaaaa ggtatttcct tacgtgctca ttggcgttgg catcggggcc
     3001 gtgattcaca actggattcc cgaagaatgg attgtagcgg tgctcggcaa caagaatccg
     3061 ttcggcgtgg tgctggcaac gctgatcggc gtaccgatgt acgcggatat cttcggcacg
     3121 attcccattg cggaagcgct gttcagtaaa ggcgcgcttc tcggctcggt gctcgcgttc
     3181 atgatggcgg ttaccacgct ttcgctgccc tccatgatca tgctccgtaa agcggtcaag
     3241 ccgcagctgc tggcgctgtt tatcgctatc tgcacggttg gcatcattct gatcgggtat
     3301 ttattcaatg cgcttcagcc gttgatcgca taggaggaca tatggcaaaa tcctggtatc
     3361 ccgtaatcga ctattccagc tgtaccgagt gcggcacttg ttcatccaag tgtccgcatc
     3421 aggtgtacga catgaaaaaa gcgcctgttc cggtggttct aaaccccgct tcctgcattg
     3481 accactgcca tggctgcgga aaccgctgtc cggtcggtgc aattacgtat gtgggggacg
     3541 ataccggctg gacgccgccg cacggcgaga tccggcagga tgaacccgcc tgcggatgcg
     3601 ggggagggag ctgctgtggc tgaagcgaaa ccggaaaaga aaaaaagccc gctgaagaaa
     3661 acgctgctgg tgattgcaat catcgcggcc attgccgtga tttggtttgt caaaaacggc
     3721 gggacgcagc aggatacgac cgctagtacg gacctgacgc aggaggactt cgcgttggag
     3781 gcggagagca tcgacctgaa cgcgctcatg gcttacgggc tgcctgtcat cattgacttt
     3841 ggctcggatt cctgtattcc gtgcaaggag atggctccaa tcctggcggc gatgaacgag
     3901 gcttttcagg gaaaggcgat catcaaattc gtggacgttt ggaaaaatgc cgacggagca
     3961 aacggctttc ctgtgcaggt tatcccgacg cagattctgt ttgacgcagc gggaaacccg
     4021 tatatcccga gcaaggactt gggcattgag tttatcgtgt acagcgataa aacgacaaac
     4081 gagcacttgt ttacggcgca tcagggcggc ttgacggagg agcagatgcg cgccatcctg
     4141 acggatatgg gagtaaccga atgatcgatc tctggctgga acagatcggc cgcgttatcg
     4201 gccagaacgc gtggcttgcg ccgctgctgg cacttcttgc gggtgttcta acttccttta
     4261 cgccctgcgc gctctccagc ataccgctcg ttgttgcata tgttggcggt atcggtgttg
     4321 aaccgaaaaa agcgttccgc ctttctctgg tgtttgcgtt cggagcggca attacgttta
     4381 ccatgctcgg ggtcgttgcc gcaagcgttg ggcaactgct tggcaccggc acaaagtggt
     4441 ggtaca
//
LOCUS       DAOMGY010000038         4410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_20410_length_4410_cov_3.043169, whole genome shotgun sequence.
ACCESSION   DAOMGY010000038 DAOMGY010000000
VERSION     DAOMGY010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20410_length_4410_cov_3.043169"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1848
                     /gene="oraE"
                     /locus_tag="PLR57_01135"
     CDS             <1..1848
                     /gene="oraE"
                     /locus_tag="PLR57_01135"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HQQ40087.1"
                     /translation="QGSGGVPISRKQVRAQRKALDLIEDEVGRPINYHSYVSGVAGPD
                     IAVMFAEEGVNGCHQDPQYNVIYRNINMVRSFVDAAESKKLIAWANMAQIDGAHNANA
                     TARDAWKVMPELMVQHAINALFSAKVGVPKSSICLSTVPPTATPGPCVYMDLPYAVAL
                     RTLFTEYRMRAQMNTKYIESSTREATVTHVLNMVISKLTSADIQSTITPDEGRNVPWH
                     MYNIEACDTAKQTLVGLDGLMEMVELKEDGYLAEKSRELIERAVLFLEEMTEKGGYFT
                     AVESGMFVDSGLFPERNGDGIVRKLDGGVGAGTVIKREADYMAPVTAHYGYNNVAQYG
                     GDPADPAALIGGCTLEKPEKIIYIDELDENDNVYLRMENVKEFAQPNAKKIRPEMEWL
                     ADGTVMLTLCFPAEKHIAEAAAIECAKRMGLKEVAVISREVLHEAEGTRIELKGKVDF
                     AIDVESLVIPAEPDVLSEEELRAAVEKRPFRVVCGTIGEDEHSVGLREIIDIKHGGIE
                     KYGIEVHYLGTSVPPEKLVGAAIELNADAMLASVIISHDDIHYKNIARIDRIAREMGV
                     REKLIFIAGGTQVEPEQAKKAGADAGFGRGTHGNHVATFLVKERAAREE"
     gene            1855..3215
                     /locus_tag="PLR57_01140"
                     /pseudo
     CDS             1855..3215
                     /locus_tag="PLR57_01140"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
     gene            3241..4311
                     /locus_tag="PLR57_01145"
     CDS             3241..4311
                     /locus_tag="PLR57_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HQQ40088.1"
                     /translation="MGEYPRITADLNKIRHNLNALIALCSEYGVSIAAVGKVVCADEK
                     IIRVFEESGVSMLADARLENLEKITTKKPKLLLRAPSPDEADETVRLSDISLVSEIDT
                     IRALGQAAKLLAKRYSIILMIDLGDLREGLLFTEREEILRTAKAATEEPWIELLGIGT
                     NLTCYGAIIPDETNLGTLCDIADEIRAATGLPVPIVSGGNSSSLGLLRRGLVPECVNH
                     LRLGESILLGNDTAACRVMNGLVGDAFTLSTVLIEVQRKPSVPIGVSGANAFGERPTF
                     TYEGEQIRGICKIGRQDTVADGLTPRDTNIRIIGASSDHLIVDLTRAKAYRVGDVLDF
                     NPDYGALLRAYTSPYVKRGYIE"
BASE COUNT         1006 a         1254 c         1351 g          799 t
ORIGIN      
        1 cagggcagcg gcggcgtacc gatttcgaga aagcaggtac gcgcgcagag aaaggcgctc
       61 gatctgatcg aggacgaggt cggcaggccc atcaactatc atagctacgt cagcggcgta
      121 gccgggccgg atatcgccgt gatgttcgcc gaggagggcg tcaacggttg ccatcaggac
      181 ccgcagtata atgtgatcta tcgaaacatc aacatggtgc gttctttcgt ggacgcggcg
      241 gagagcaaaa agctcatcgc ctgggcaaat atggcgcaga tcgacggcgc gcataacgcc
      301 aacgccaccg cgcgggacgc ctggaaggtc atgccggagc tcatggtgca gcacgccatc
      361 aacgctctgt tctcggcaaa ggtcggcgtg ccgaagtcga gcatctgtct ttccaccgtg
      421 ccgccgacgg ctacgcccgg gccctgcgtc tatatggatt tgccgtacgc ggttgcgctg
      481 agaacgctct ttacggaata ccgcatgcgc gcgcagatga acacgaaata tatcgagtcc
      541 agcacgcggg aagcgaccgt gacgcatgtg ctcaacatgg ttatcagcaa gctcaccagc
      601 gcggacattc agtccactat cacgccggac gaagggcgca acgtcccctg gcatatgtac
      661 aatatcgagg cctgcgatac cgccaagcag acgctggtcg gcctcgacgg gctcatggag
      721 atggttgagc tcaaagaaga cggctatctt gcggagaagt cccgcgaact tatcgagcgc
      781 gcggtgctct tcctcgaaga gatgaccgaa aaaggcggct attttacggc ggtcgagagc
      841 ggcatgttcg ttgacagcgg attgttcccg gagcgcaacg gcgacggcat cgtccgtaaa
      901 ctcgacggcg gcgttggagc gggaacggtc atcaagcgcg aagcggacta tatggcgccc
      961 gtcaccgcgc attacggtta caacaatgtt gcacagtacg gcggcgaccc cgccgaccct
     1021 gccgcgctga tcggcggctg cacgctcgaa aagccggaaa agatcatcta tatcgacgag
     1081 ctggacgaaa acgacaatgt ttatctccgt atggagaacg tcaaggagtt tgcccagccg
     1141 aacgcaaaga agattcgccc cgaaatggag tggctggcgg acggaaccgt aatgctgaca
     1201 ctctgcttcc cggctgagaa gcatattgcc gaagccgctg ccatcgagtg cgcaaaacgg
     1261 atggggctga aagaggtcgc cgtcatctcc cgcgaagtac tgcacgaagc ggaaggcacg
     1321 cgcatcgagc tcaagggcaa ggtagatttt gctatcgacg tcgaatcgct ggtcattccc
     1381 gccgagccgg acgtgctgag cgaagaagag cttcgcgcgg ctgtggaaaa acgcccgttc
     1441 cgcgtcgtct gcggcaccat cggcgaggac gagcacagcg tgggtctaag ggagatcatc
     1501 gatatcaagc atggcggcat cgaaaagtac ggcatcgaag tgcattacct cggcacatcc
     1561 gtgccgccgg aaaagctcgt tggtgcggcc atcgaactca acgcggacgc gatgctggct
     1621 tcggtcatca tttcgcatga cgacatccac tataagaata tcgcccgcat cgaccgcatt
     1681 gcgcgggaga tgggcgtgcg cgaaaagctc atctttatcg cgggcggcac gcaggtcgag
     1741 ccggagcagg cgaaaaaagc cggcgcggac gcgggctttg gacgcggcac gcacggcaat
     1801 cacgtcgcga cgttcttagt aaaagaacgc gccgcgcgcg aagaataatt tatcatgtat
     1861 acggacgtac tcatagcgga aatcggctcg accaccacgc tcgtcagcgc gtttgacggg
     1921 ctggaaggcg agccgcgctt tttggggcag ggtcaggcgg cgaccaccgt gctcgaaggg
     1981 gacgtccgga tcggtttatc cagcgccatc cgcgcgctga aagagtcgct caaaacggat
     2041 acgctcgact acggcagcct gcttgcttcg tccagcgcgg cgggcgggct caagatatgc
     2101 gtccacgggc tggtgtacga catgacggtc agcgcggcca aggcggcggc gctcggcgca
     2161 ggcggaatcg tccgccagac cacagcgggc aggctcaccg cacgcgacct ggaagaacaa
     2221 aagcagatca aaccaaacct catcctgctc gcgggcggca cggattacgg cgagcgcgaa
     2281 accgcgctgt acaacgcgga actgctggta aagtcgaata tcggcgcgcc ggttctctac
     2341 gcgggcaacg tgcagaacca gtccgcagtg aaagagatct tcgagtctgc cggaatcccc
     2401 gtctatctga cggaaaacgt ctatccgcgc ctcgacgcgc tgaatattga acccgtgcgc
     2461 aaggtcatac acgccgcgtt cgagcagcat atcgtctcgg ctcccggcat ggagcatatc
     2521 cgcgacctcg tgacgggcac aattctgccg acgcccggcg cggtgatgga agcggcaatg
     2581 ctgctctatg aggagattgg cgatttactc gtgctcgata tcggcggcgc gacaacggac
     2641 gtacactccg ttacaaacgg ttcggaggag attgcggcgc tgttgaccgc gccggaaccc
     2701 ttcaacaagc gcacggtcga gggcgactta ggtttgtacg tcaatgcgcg tcgtctgatc
     2761 gagatgatag gggaagagaa gctttcaaga gagcttggac tggatatccc cgacgtgatg
     2821 cgggaataca aaccgattcc cgaaacgaaa gagcagtttt tgctgaccaa ccggctctgc
     2881 ctcgaagcgg ggcttaccgc tattatgcgt cacgcgggca ggctacggca cttttacacg
     2941 ccgcagggac ggcgaccgcc gccgagggta aagaccttac gcaggtgaaa acgctcatcg
     3001 gcaccggcgg cgctttgacc cgcctgccgg agcgcgagcg gattgcgcgt gcgctcgcgg
     3061 actgcaatca gggcggaacg atgctctacc ccaaaccggg agccatgaag accgcgttcg
     3121 acgaatcgta tgtcatggcg tctctgggcg tcctctctaa aacccgtccg gacgcggcaa
     3181 agatattact gaaacaaacg ctccgatttg cataaaaccg gaaaatcaag gggaacttct
     3241 atgggtgaat acccgcgcat tacggcggac ctaaataaaa tacggcataa cctcaacgcg
     3301 ctgattgcgc tttgttcgga gtacggcgtt tccattgcgg cggtgggaaa agtcgtctgt
     3361 gcggacgaaa aaatcatccg cgtgttcgag gagagcggag ttagcatgct cgccgacgcg
     3421 cggctggaaa atctcgagaa aatcacaaca aaaaaaccga agctgctgct ccgcgcaccc
     3481 tcaccggacg aggcggacga gacggttcgg ctttcggata tcagccttgt ttcggaaatc
     3541 gacaccatcc gcgcgctggg acaggcggct aagcttctgg caaagcgcta ctcgatcatc
     3601 ctgatgatcg accttggcga cctgcgcgaa gggcttttat ttaccgagcg ggaggaaatc
     3661 ctgcgcacgg cgaaggccgc tacggaagaa ccttggattg aactgctggg cattggcacg
     3721 aacctcacct gctacggcgc gattattccc gacgaaacga acctcggcac actctgcgat
     3781 attgcggacg aaattcgcgc ggcaacggga ctgcccgttc ctatcgtctc cggtggaaac
     3841 tccagctcgc tggggctgct gcggcggggg cttgtgccgg aatgcgtaaa ccatctgcgg
     3901 cttggcgaat cgattctgct gggcaacgac accgccgcct gccgcgtgat gaacggtctg
     3961 gtgggagacg cgtttacctt gagcacagtt ctcattgaag tgcagagaaa accctccgtg
     4021 ccgattggcg taagcggcgc gaacgccttt ggcgagaggc cgacctttac gtacgaaggc
     4081 gaacagattc gcggcatctg caagatcggc cgccaggaca ccgttgccga cgggctgacc
     4141 ccgcgggata cgaacatccg catcatcggt gcgagttcgg atcatttgat tgtcgatctc
     4201 acgcgagcca aagcgtatcg agttggcgac gtgctggact ttaatccgga ctacggcgcg
     4261 cttcttcggg cgtacaccag cccctatgta aagagaggtt atatcgaata aaaaaagggc
     4321 ttaagccctt tttttattcg atttgaaatt tagttgagcg ccgtacagac ccgttcgaga
     4381 ccttctttga gcacgctcca ggggcaagcg
//
LOCUS       DAOMGY010000039         4408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_20423_length_4408_cov_4.018148, whole genome shotgun sequence.
ACCESSION   DAOMGY010000039 DAOMGY010000000
VERSION     DAOMGY010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20423_length_4408_cov_4.018148"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLR57_01150"
     CDS             <1..635
                     /locus_tag="PLR57_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQQ40089.1"
                     /translation="AYVDYALKNGTLILYDSAYESYISDPSLPHSIYEVEGAKECAIE
                     FRSMSKTAGFTGTRCAYTVVPKALVCKDSFGNEVHLRDLWARRVSTKFNGVAYITQRG
                     AEAAYSKVGNEEIMEVVAYYMENARIIREGLENAGYKVFGGVNAPYIWLKVPHGDSWK
                     FYDELLSQYNIIGTPGEGFGAAGEGFFRMTAFATRESTIKAMERIKKGGV"
     gene            632..1297
                     /locus_tag="PLR57_01155"
     CDS             632..1297
                     /locus_tag="PLR57_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40090.1"
                     /translation="MTKIIAFEGIDGTGKTVQLERQANRLAALGRKVKTISFPVYESF
                     FGAEVGRLLSGSGGVKASDVDGRSMALWYALDRFEAFQTFEYQDAGVLLINRYLLSNA
                     VYQSVRERDLGKPDLLEFVLELEYEHFRIPRADAHIVLDMNPENASENVGKKGYREYL
                     GDRKDVYEEIPSLQERARLKYLEYAERMEGIRIIPCMENSKLKSIEAIGLLIDHEVDD
                     ILL"
     gene            1407..2468
                     /locus_tag="PLR57_01160"
     CDS             1407..2468
                     /locus_tag="PLR57_01160"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HQQ40091.1"
                     /translation="MRKIGILTGGGDCPGLNAVIRGVVEECAANGIEVYGFSAGWRGA
                     LTGEGRWLSLADVSGLQTKGGTILESSRTNVMKEENGPERVRKTLESLGLEGLVAIGG
                     DDTLGVANKLRKMGMKMIGVPKTIDNDLSCTDYTFGFDTASNIAMECIDRLHTTAASH
                     ARCIVVECMGRHTGWIAVQAGLAADANLILIPEFPKTFDEIMQVIRARYIKGERHTII
                     AVAEGFELKGETDEDVGLDAFGNKLLLEKEIAKTLATRIEKAMRADASLDEKRKYFEC
                     RSVVLGHIQRGGSPSCFDRVLGTRLGFKAGELVVSEQYGNMVALQGTSIVTADLDKAV
                     TERKEVDWDFYKSVSILFK"
     gene            complement(2570..4390)
                     /locus_tag="PLR57_01165"
     CDS             complement(2570..4390)
                     /locus_tag="PLR57_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009983738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40092.1"
                     /translation="MGGGPRGFLTEEEKLNRPKLSKALLLRILAYLKPYKIQFVFVFA
                     AILISAVIGLFPSLITGKIVDEALVGKDMALLIKLLLLALVTLTASQAIGVLQSYINS
                     WISQRIIFDMQNQMYAHLQQMPHSFYTTEKQGDILTRMDNDISGVGTVITNTLTTSVA
                     NVAIMIATLIALFSLNWKMALIGIAIIPLTLIPARRVGQTRWKLLADVQAERDNMNQM
                     LTETLSVSGSMLMKLFTREKSEYDRFVSVNERVTKLTLKEQRSGKWFVMILGTLSQID
                     PLLIYFMGGWLIISKTDTALTIGTITAMIALVNRLYGPVNSLLNLGVDFTRSLALFTR
                     IFGYYDKEVTIKSKQDAVQPDLEQTEISFRHVTFTYEQKEPILKDISFTIPSGSMVAI
                     VGPSGAGKSTIVNLLLRLYDVNAGEITVAGTDIRDFDLTYLRSNIGVVTQDAYLFNGT
                     IRENLLYAKPDATQQEMEAACRLANIHEFIAAQEKGYDTMVGNRGLKLSGGEKQRISI
                     ARVALKNPKILVLDEATSSLDSISENAIQNALEAVMRGKTSVVIAHRLTTVLAADWIL
                     VVKDGIICEQGTHDRLLEQNGVYRELYETQFKRVLEHEGY"
BASE COUNT         1015 a         1227 c         1229 g          937 t
ORIGIN      
        1 aagcgtatgt cgattacgcg cttaaaaacg gcacgctgat tctctacgat tccgcttacg
       61 aatcctatat ctccgatcct tctcttccgc acagcattta cgaagtcgaa ggcgcaaagg
      121 aatgcgccat cgagttccgc tccatgtcca agaccgcggg ctttaccgga acgcgctgcg
      181 cgtatacggt ggttcccaaa gcgctggtct gcaaggactc gtttggcaac gaagtgcacc
      241 tgcgtgacct ctgggcgagg cgtgtttcca ccaagttcaa cggcgttgcc tatattacgc
      301 agcgcggcgc ggaagccgcc tacagcaaag tgggcaacga agagattatg gaagttgttg
      361 cgtattacat ggagaacgcg cgcattatcc gcgagggtct cgaaaacgcg ggatacaagg
      421 tgtttggcgg cgtcaacgcg ccgtatatct ggctcaaagt tccgcatggc gacagctgga
      481 agttctacga cgaactgtta agccagtaca atatcatcgg cacccccggc gaaggtttcg
      541 gcgcggctgg cgaaggcttt ttccgcatga cggctttcgc cacgcgcgaa agcaccatca
      601 aggccatgga acgcatcaaa aagggcggcg tatgacaaag attatcgcgt ttgaaggcat
      661 cgacggaacg ggtaaaaccg tacagctcga acggcaggcc aatcgccttg ccgcgctggg
      721 acgcaaggta aaaacgatct ccttccccgt ctacgaatcg tttttcggcg cggaagtcgg
      781 gcggctcctc tccggcagcg gcggcgtaaa agccagcgac gtggacggca ggagcatggc
      841 gctctggtat gcgctcgacc ggttcgaagc gtttcaaacg tttgaatatc aagatgcggg
      901 cgtgcttctc atcaaccgct accttctcag caacgccgtc taccagtccg tgcgggaacg
      961 cgatctcggc aagcccgacc tgctggagtt tgtactggag ctggaatacg agcacttccg
     1021 cattccacgc gcagacgcac atatcgtgct cgatatgaac ccggaaaacg catccgaaaa
     1081 cgtcggcaaa aagggctacc gtgaatactt aggcgaccgc aaggacgtct acgaggaaat
     1141 ccccagtctg caggagcgcg caagactcaa gtatctggag tatgccgaac gcatggaagg
     1201 cattcgcatc atcccctgta tggaaaacag caagctcaag agcattgaag ccatcgggct
     1261 tttaatcgat catgaagtgg acgatatcct gctgtaacgc aggtgcagtc acaaagcagg
     1321 tcaggtcact gagcacactc gattttttca gtctgccaaa cagactgcag aagatatcca
     1381 atagatcaaa caggaggtaa cacaatatga ggaaaattgg tatcctgacc ggcggcggag
     1441 actgcccggg tctgaacgcg gttatccgcg gcgttgtgga agaatgcgcc gcaaacggca
     1501 tcgaagttta cggtttttcg gccggctggc gcggcgcgct gaccggtgaa ggccgctggc
     1561 tttcgctggc cgacgtaagc ggtctgcaga cgaagggcgg caccattctg gagagctccc
     1621 gcaccaacgt catgaaggaa gaaaacggcc ccgagcgtgt ccgtaagacc ctggaaagcc
     1681 tcggtctgga aggccttgtc gccatcggcg gcgacgacac gttgggcgtt gccaacaagc
     1741 tccgcaagat gggcatgaag atgatcggcg tgccgaagac catcgacaac gacctctcct
     1801 gcacggacta caccttcggc tttgacaccg cgagcaacat cgccatggaa tgcatcgacc
     1861 gtctgcacac caccgccgcg agccacgcgc gctgcatcgt ggtcgagtgc atgggtcgcc
     1921 acaccggctg gatcgccgtt caggcgggtc ttgccgcgga cgcgaacctg attctgattc
     1981 ccgagttccc caagaccttt gacgagatta tgcaggttat ccgcgcgcgc tacatcaagg
     2041 gcgagcgcca caccattatc gccgttgcgg aaggctttga gctcaagggc gaaacggacg
     2101 aggacgtcgg cctcgacgcg ttcggcaaca agctgctgct cgaaaaagag attgcaaaga
     2161 cgctggccac ccgcattgaa aaagccatgc gcgcggacgc ttcgctggat gaaaagcgca
     2221 agtacttcga gtgccgttcg gtcgttctgg ggcatatcca gcgcggcggt tccccctcct
     2281 gcttcgaccg cgtgctcggc acccggctgg gcttcaaggc gggcgaactg gtcgtttccg
     2341 agcagtacgg aaacatggtc gcgctccagg gcacgagcat tgtaaccgcc gacctcgaca
     2401 aggccgtcac cgagcgcaag gaagtcgatt gggactttta taagtccgtt tcgatcctgt
     2461 tcaaataaaa actgaatcaa ttcagagcta ctataaatcc gtttcaatac tttttaaata
     2521 aaacaaacta aaaaacgaac cgcccgtgaa aacgggcggt tcgttttaat taataccctt
     2581 cgtgctccag aacgcgcttg aactgcgttt catacagctc cctgtatacg ccgttctgct
     2641 ccagcaaacg gtcatgcgtt ccctgttcgc agatgattcc gtcctttacg acgagaatcc
     2701 agtctgccgc gagaacggtt gtaagcctgt gcgcaatgac cacgctggtt tttccgcgca
     2761 ttacggcttc cagcgcgttt tgaattgcgt tttccgaaat ggagtccagc gacgaggtcg
     2821 cttcgtccag cacgagaatc tttgggttct tgagcgcaac cctcgcaatg gagatgcgct
     2881 gcttttcccc gccggaaagt ttgagcccgc ggtttccgac catggtgtcg tagccttttt
     2941 cctgagcagc gataaactcg tgaatattgg cgagtctgca agcggcttcc atctcctgtt
     3001 gggtcgcatc cggtttagca tacaagaggt tttcgcggat cgtaccgtta aaaagatacg
     3061 cgtcctgcgt gacaacgccg atgttcgacc gaagataggt cagatcgaaa tcccgaatgt
     3121 ccgtacctgc aacggtgatt tcacccgcat ttacgtcgta tagacgcagc aggaggttga
     3181 cgattgtgct cttgcccgcg ccggaaggcc ccacgatggc gaccatgctg ccggaaggaa
     3241 ttgtaaagct gatatccttg aggatcggct ccttctgctc gtaggtgaac gtaacgtgcc
     3301 gaaagctgat ctccgtctgc tccaaatccg gctgaaccgc gtcctgcttg ctcttgatcg
     3361 tgacttcctt atcgtaatag ccgaagatgc gcgtaaacag cgcaagtgag cgcgtaaagt
     3421 ccacgcctag attcagcagc gaattcaccg gcccgtacag ccgattaacc agcgcgatca
     3481 tggccgtgat tgtgccaatg gtcagcgcag tatcggtttt ggaaatgatc agccaaccac
     3541 ccataaagta aatcaggagc gggtcgattt gcgacagcgt tccgaggatc atgacaaacc
     3601 acttgccgct gcgctgttcc ttgagcgtga gtttggtcac gcgctcgttg accgagacga
     3661 accggtcgta ctcggatttt tcgcgcgtaa agagcttcat gagcatggaa ccggagacgc
     3721 tcagcgtttc cgtgagcatc tggttcatgt tgtcgcgctc ggcttgaacg tccgcaagga
     3781 gcttccagcg cgtttgcccg acgcgccgcg cgggaatcag cgtgagcgga atgatggcga
     3841 ttccgatgag cgccatcttc cagttcagcg aaaacagcgc gatcaatgtt gcgatcataa
     3901 tcgccacgtt cgccaccgac gtggtgagcg tgttggtaat caccgtgccg acgccgctga
     3961 tgtcgttgtc cattcgcgtc aggatatcgc cctgcttttc ggtcgtgtaa aacgagtgcg
     4021 gcatctgctg cagatgtgcg tacatctgat tttgcatgtc gaagatgata cgttgggaaa
     4081 tccaggagtt gatgtagctc tgcagtacgc cgatcgcctg cgaagcggtg agcgtgacca
     4141 gcgccagcaa cagcagtttg atgagcagcg ccatatcctt gccgaccagc gcttcgtcaa
     4201 cgattttccc tgtaatcagg gaaggaaaca ggccgatcac cgcgctgatg agaattgccg
     4261 caaacacaaa aacgaactga attttatatg gtttcaggta cgccaaaatc cgaagcagca
     4321 gggctttgct gagcttcgga cgattcagct tttcttcttc cgttaaaaat ccgcgcggcc
     4381 ccccaccaat gctgggacct ggtctacc
//
LOCUS       DAOMGY010000040         4402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_20464_length_4402_cov_3.196687, whole genome shotgun sequence.
ACCESSION   DAOMGY010000040 DAOMGY010000000
VERSION     DAOMGY010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20464_length_4402_cov_3.196687"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1180)
                     /gene="mnmA"
                     /locus_tag="PLR57_01170"
     CDS             complement(65..1180)
                     /gene="mnmA"
                     /locus_tag="PLR57_01170"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009592778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQQ40093.1"
                     /translation="MNNELHTGHVVVGMSGGVDSAVAASLLKEQGCDVVGVFMKNWEE
                     EDEENGVCTAASDYEDVKSVCNAIGIPYYTVNFAKEYRDRVFSYFLEEYRRGRTPNPD
                     VLCNCEIKFKAFLDFAVSTGAGALATGHYARLDKSGETVKLLRAADANKDQTYFLSGL
                     TQEQLKNAIFPIGGLVKSEVRRIARERGFSNAAKKDSTGVCFIGERNFKQFLMQFLPA
                     QPGDMVDETGRVIGRHDGLMYYTLGQRRGLGIGGRNDGTGESWFVIGKDLNRNLLIVQ
                     QGEHDELFSLSLDAETIQWIAGEPPKSEFACTGKFRYRQPDQGVFVTAEGAGVHVRFD
                     KPQRAVTPGQWVVLYAGDVCLGGGPIDQVEPKKGISI"
     gene            complement(1257..1472)
                     /locus_tag="PLR57_01175"
     CDS             complement(1257..1472)
                     /locus_tag="PLR57_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HQQ40094.1"
                     /translation="MTTYLLTVDGMGCSSCAGRVDLALKEAGFTVLSTQVGSAKIQSS
                     MNVDTIKKHVSEILEDMGYMLTGIKAE"
     gene            1740..3161
                     /locus_tag="PLR57_01180"
     CDS             1740..3161
                     /locus_tag="PLR57_01180"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HQQ40095.1"
                     /translation="MENSATESKESAKKFTMDQIVALCKGRGFVYPGSEIYGGLANSW
                     DYGPLGVEFKNNIKRAWWRKFVQESPYNVGMDAAILMNPETWVASGHVGGFSDPLMDC
                     KACKARFRADKLIEDYAAEHGLTDIRPDGWTNAQMEAYIREQGIVCPECGKTEFTGIR
                     KFNLMFKTFQGVTEDTASELYLRPETAQGIFVNFKNVLRTTRRKLPFGIAQIGKSFRN
                     EITPGNFIFRIREFEQMELEFFCAPGTDLEWYHYWKDYCHKFLLSLGMQDEKIRLREH
                     AKEELSHYSKGTTDIEFLFPFGWGELWGVADRTDFDLKSHATHSGESFEYLDPFTNEK
                     FIPYCVEPSLGADRVALAFLCNAYDEQELEGGDVRTVLHLHPALAPYKCAVLPLSKKL
                     AEQAQPVYAKLAKRFMVDYDDAGSIGKRYRREDEIGTPFCVTVDFDTETDGCVTVRDR
                     DTMEQVRLPIDELTAYIEARLDF"
     gene            3276..>4402
                     /locus_tag="PLR57_01185"
     CDS             3276..>4402
                     /locus_tag="PLR57_01185"
                     /inference="COORDINATES: protein motif:HMM:NF025843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQQ40096.1"
                     /translation="MAEISGVVNSIIYRKSENGWTVLELTPDEGEKLTVVGALPLASV
                     GERVEFAGDYASHPKYGSQFKAYSYKTLAPATLSAIENYLGSGLIKGIGAATAKAIVA
                     KFGMDTLSILDETPARLAEIPGIGAKRRDMILASYTENRMMRDIMLALEPYGVTVSQA
                     YKLYKIYGELCLSRIEENPYQIIRDVDGIGFVTADKIAQNVSGFAFDSESRLRAGILY
                     ALQSASGEYGHTFLPKESLISYAAKLLGVPQERVLETLDILIERGEAILQFVGETEGV
                     FLPYLQRMEINIAEKLLNLSQKPVDNPFFDFEVAQASQKLELSAQQRDAVLAALNEGA
                     LVITGGPGTGKTTIIRFIRAIMESMGLSVELTAPTGRAAKR"
BASE COUNT          965 a         1314 c         1218 g          905 t
ORIGIN      
        1 cagaaaagtt tatatcgtga gtttagtggt gctgcccttc gtcgggagct tgagggcgga
       61 gccctcaaat cgatataccc ttcttcggtt ccacctgatc gatcgggccg ccgccgaggc
      121 aaacgtcgcc tgcgtacagc acaacccact gccccggcgt gaccgcgcgc tgcggcttgt
      181 cgaaacgcac atgcacgccc gcgccctccg ctgtgacaaa cacgccctga tccggctggc
      241 ggtagcggaa cttgcccgta caggcaaact cgctctttgg cggttcgccc gcgatccatt
      301 ggatggtttc cgcatccagc gagagcgaaa acagctcgtc gtgctcgcct tgctgaacga
      361 ttaagagatt gcggttcagg tctttgccga tcacaaacca actctcgccc gttccgtcgt
      421 tgcgcccgcc gatgccaagc cctctgcgct gaccgagcgt atagtacatc agcccgtcgt
      481 gccggccgat gacccgcccg gtttcgtcca ccatgtcgcc cggctgggct ggcagaaact
      541 gcattaaaaa ctgcttgaag ttccgttccc cgataaagca aacgccggtc gaatcctttt
      601 ttgccgcgtt ggaaaagccg cgctcacgcg cgatgcgccg cacttcgctt ttgaccagcc
      661 ccccgatggg gaaaattgcg tttttcaatt gttcctgcgt taagccggag agaaaatagg
      721 tctgatcctt attggcgtcc gcggcgcgga gaagttttac cgtttcgccc gatttatcca
      781 gacgcgcata atgccccgtc gccagcgcgc ccgcgccggt ggagacggca aagtccagaa
      841 acgccttgaa cttgatttca cagttgcaca aaacgtccgg attaggcgtg cgcccgcgcc
      901 ggtattcctc gagaaagtac gaaaaaacgc ggtcgcgata ttcctttgca aaattgaccg
      961 tataataagg gattcctatg gcgttgcaga cgctttttac gtcctcataa tcactggcgg
     1021 cggtgcagac gccgttttcc tcgtcttctt cctcccagtt tttcatgaac acgccaacga
     1081 cgtcgcatcc ctgctctttg agcagggacg cggcgaccgc gctgtccacg cctccggaca
     1141 tgccgaccac gacatgcccg gtatgcaatt cattgttcaa actgatgctg cctcaaattg
     1201 cctgttgaat ttcgaaagcg aatcggaaaa gcgtttgagc ggcaatcgca atattgttat
     1261 tccgccttga tacctgtcag catatatccc atgtcttcga gaatttcgct tacgtgcttt
     1321 ttgatcgtat caacattcat ggaagactga atctttgcgc tcccaacctg ggtggaaaga
     1381 acggtgaatc cggcttcctt gagcgcaaga tcaactcttc cggcgcagct ggagcaaccc
     1441 attccgtcaa cagtcagcag ataagtagtc ataaacggta ccctccttgg ttttctttag
     1501 gctactataa cacacaggat tttttgcgtt cgatgactaa gtcacacttc tcggcttcgc
     1561 ttcgatagaa ttattgtacc gttttctgcg cgccagagca agaagcaggg cgcagcaagg
     1621 tcaaacgcgc gaaaaatgcc gctgtaccgc cctctctcag acgaccgcgc gccttgacaa
     1681 aagcgcgtat ttcctgcgat aatatgcgtt gactgacaag caaggagcat gaaacaacca
     1741 tggagaacag cgccaccgaa tcaaaggaat cggcaaagaa atttacaatg gatcagatcg
     1801 tcgccctgtg caaggggcgc ggttttgttt accccggcag cgagatctac ggcggtctcg
     1861 cgaactcctg ggattacggc cctctgggcg tggaattcaa aaacaacatc aagcgcgcat
     1921 ggtggcgcaa gtttgttcag gaatcgccct acaacgtggg catggacgct gccatcctca
     1981 tgaacccgga aacctgggtc gccagcggac acgtgggcgg tttttccgac ccgctgatgg
     2041 actgcaaggc ttgcaaagcg cgtttccgcg cggacaagct gattgaggac tacgctgcgg
     2101 agcacggcct tacggacatt cgcccggacg gctggacgaa tgcgcagatg gaagcgtata
     2161 tccgcgaaca gggcatcgtc tgccccgaat gcggcaaaac cgaattcact ggcatccgca
     2221 agttcaacct gatgttcaaa acatttcagg gcgtgaccga ggataccgcg tccgagctct
     2281 atctccgccc cgagaccgcg cagggcatct tcgtcaactt caaaaacgtc ctccgcacga
     2341 cccgccgtaa actgccgttc ggtattgcgc agatcggaaa atccttccgc aacgagatta
     2401 cgccgggcaa cttcatcttc cgtatccgcg agttcgaaca gatggagctg gagttcttct
     2461 gcgcgccggg caccgatttg gaatggtacc actactggaa ggactactgc cacaaattcc
     2521 tactctctct gggcatgcag gacgaaaaga ttcgcctgcg cgaacacgcc aaggaagagc
     2581 tttcgcacta cagcaagggt acgacggata tcgagttcct cttcccattc ggctggggtg
     2641 aactctgggg cgttgccgac cgcacggact ttgacctcaa gagccacgcc acgcacagcg
     2701 gcgagagctt tgaatacctc gacccgttca cgaacgaaaa gttcatcccc tactgcgtcg
     2761 agccgtcgct gggcgcggat cgtgttgcgc ttgcgttcct ctgcaacgcg tacgacgagc
     2821 aggagctgga aggcggggac gtccgcaccg ttctgcacct ccaccccgcg ctcgcgccgt
     2881 ataaatgcgc cgttctgccg ctgagtaaga agctcgccga gcaggcgcag cccgtctacg
     2941 ccaagctcgc aaagcgcttt atggtggact atgacgacgc gggttccatc ggcaagcgtt
     3001 accgcagaga ggacgaaatc ggcacgccgt tctgcgtcac cgtcgatttt gacaccgaga
     3061 ccgacggctg cgtgaccgtc cgcgaccgcg acacgatgga gcaggtgcga ttgcccatcg
     3121 acgagctgac cgcttacatc gaagcgcgcc tagacttcta aagccttatg catcgccccc
     3181 gccgcaaacg cgggggcatg ttttattgca acgtatcata gatacagcaa gcgcatgcgg
     3241 gctaacgcct gagcgcctga cacgcgggag cgaacatggc ggaaatcagc ggagtcgtca
     3301 attccattat ctaccgcaag agcgaaaacg gctggacggt gctggagctt acccccgacg
     3361 agggcgaaaa gctcacggtc gtcggcgcgc tgccgcttgc ttccgttggc gaacgcgttg
     3421 agttcgcggg cgactacgcg tcgcacccca agtatggcag ccagttcaaa gcatacagct
     3481 acaaaaccct tgcgcccgcc acgctgagcg cgattgaaaa ctacctcggc agcgggctga
     3541 ttaaggggat tggagcggcc acggcaaagg ccatcgtcgc caagttcggt atggacacgc
     3601 tctccattct ggacgaaacg cccgcgcggc tggcggaaat ccccggcatc ggcgcaaaac
     3661 ggcgggatat gatcctcgcc agctacacgg aaaaccgcat gatgcgggat atcatgctgg
     3721 ccctggagcc ttacggcgtc accgtcagcc aggcttacaa gctttacaaa atctacggtg
     3781 agctttgcct cagccgcatt gaggaaaacc cgtaccagat cattcgggac gtggacggca
     3841 tcggctttgt gaccgcggat aagattgccc agaacgtttc cggctttgcg ttcgactccg
     3901 aatcccgcct ccgcgcgggg attctctatg cgctgcagag cgcgagcggc gaatacgggc
     3961 atacgtttct gccgaaagag tcgctgatca gctacgcggc gaagctcctc ggcgtgccgc
     4021 aggaacgcgt gctggagacg ctggatatcc tcattgagcg cggggaggcg attctgcagt
     4081 ttgttggcga aacggagggc gtgttcctgc cgtatctcca gcgcatggag atcaatattg
     4141 ccgaaaagct gctgaacctt tcgcaaaagc ccgtcgataa cccgtttttc gattttgaag
     4201 tggcgcaggc ttcccaaaag ctggagcttt ccgcccagca gagggatgcg gtgctagccg
     4261 cgctcaacga aggcgcgctg gtcatcaccg gcggccccgg cacgggcaaa acgacgatta
     4321 tccgttttat tagggccatt atggagagca tggggctctc cgttgaactc accgcgccga
     4381 ccgggcgcgc ggcaaagcgc at
//
LOCUS       DAOMGY010000041         4353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_20791_length_4353_cov_2.960214, whole genome shotgun sequence.
ACCESSION   DAOMGY010000041 DAOMGY010000000
VERSION     DAOMGY010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20791_length_4353_cov_2.960214"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..855
                     /locus_tag="PLR57_01190"
     CDS             100..855
                     /locus_tag="PLR57_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HQQ40097.1"
                     /translation="MIELDIPITPMQAYLNEKRKQGYRFSHLGVVLAAYVRTAAEYPL
                     LNRFIANKRIYQRNEFSVAMVVLKPGELDGTMNKMYFNMNDDIFTVHETIDRYVSENR
                     EEGDTNSTDDIVRKLLSIPGLCNFGVGLLKMLDKFGWLPKSLIHASPFHTSLTISNLA
                     SIRTNHIFHHVYEFGTTSILITLGNMREVPVRKGEEIVFERSLPMGVVMDERICSGSY
                     FAAVFRRFKQYLTNPALLEEPPKVVNTVLGQGE"
     gene            1063..1443
                     /locus_tag="PLR57_01195"
     CDS             1063..1443
                     /locus_tag="PLR57_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HQQ40098.1"
                     /translation="MQKKIIATTAAPAAIGPYSQAVSANGFLYLSGMLAIDPQTGSLV
                     GEDAPSQAKQILKNISALLASEGLSIENVIKTTVFLTDLSAFGAVNAVYGETFAKNPP
                     ARSCVEVSRLPKDALVEIECVAVY"
     gene            1486..>1775
                     /locus_tag="PLR57_01200"
     CDS             1486..>1775
                     /locus_tag="PLR57_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQQ40099.1"
                     /translation="MIDLFSKKHVVFDGAMGTLLLEAGLPAGASPDSWNITHPDAILS
                     IYKAYLEAGAEVITTNTFGSNKPRQKRGSYTPAQLAEAGVRLARQAVEASGK"
     assembly_gap    1776..1875
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1876..2501
                     /locus_tag="PLR57_01205"
     CDS             <1876..2501
                     /locus_tag="PLR57_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQQ40100.1"
                     /translation="AEAGVRLARQAVEASGKDCVVALDIGPLGEFIEPMGDLTEEEAI
                     ELFREPIEAGVAAGADCIVIETMCDLTEALCAVTAAKQYGGGLPVICTLSYDPNGRLM
                     TGVTLEKVVSALEEAGVTALGCNCGVGPAQLVDLLPRFTACAHIPLVMQPNAGLPEFR
                     DGQTVYLVGPEEYSSLMKTLSDGGVWGLGGCCGTTPAHIQALVRAIR"
     gene            2588..3517
                     /locus_tag="PLR57_01210"
     CDS             2588..3517
                     /locus_tag="PLR57_01210"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HQQ40101.1"
                     /translation="MQNLVYILSVVLPLLVALGLGFYTRKKNILSPQAIEGMKTFVVK
                     FALPALLFGLFFTAEFESSLLVFSATTFVLGYLGLGFGILVAKPFVKRSPMLQYMNVG
                     WEVGLLGYSLFTLLFGAENLRYMALMDFGQVLFVFSGFFTLLYARNGQGLKESFKSLL
                     RNPIPWSMLLGVAFAASGASKALEPSGIASLIVSICNFLSAPLSCLIFIVVGYGIEFN
                     KKNIGTAVISAILRTFVCSILCLVSLFVVGKFLVLSEAMRWAVIMFYLAPAPYVLTIF
                     ALNDQERADISMSLTMQTIVSISAFILIVLFRG"
     gene            complement(3585..>4353)
                     /locus_tag="PLR57_01215"
     CDS             complement(3585..>4353)
                     /locus_tag="PLR57_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HQQ40102.1"
                     /translation="IFSELYNEGNIPPVEQALASKYAENKHFGKPSGLMDQLACAVGG
                     FIAIDFEEPEHPRIEKIDRDLAELGYAICIVNCGGNHADLTPEYASIPAEMGAVARFF
                     GKTVLREVEESAFYAALPELRKTVSDRAILRAIHFFAENERVVKQADALKNGDAEAFR
                     LLMNESGRSSFQYLQNVCPGDPSERGLALALALSERLLNGVGAWRVHGGGFAGTIQAL
                     VPLDRVEAYRAEIERVLGEGSCYRFAVRPVGGVMLRA"
BASE COUNT          863 a         1273 c         1177 g          940 t
ORIGIN      
        1 ggtgattccc atgcgtaagg acggaacccg cgtcaagaac gcaaacccca tctataccgt
       61 catgcctaca tccttaagta tcgctacgat gcgatgaaca tgatcgagct ggatatcccc
      121 atcacgccca tgcaggcgta tttgaacgaa aagcgcaagc agggctatcg tttttcgcac
      181 ctcggggtcg tgctcgcggc gtatgtgcgc accgcggcgg aatacccgct gctcaaccgc
      241 tttatcgcca acaagcgcat ctaccagcgc aacgaattct ccgtcgctat ggtggtgctt
      301 aagcccggcg agctggacgg cacgatgaac aagatgtact tcaacatgaa cgacgacatc
      361 ttcaccgtgc acgaaaccat cgaccgctac gtttcggaaa accgcgagga gggcgacacc
      421 aactcgacgg acgacatcgt ccgtaaactt ttgagcattc cggggctctg taactttgga
      481 gtagggttgc tcaaaatgct cgataaattc ggctggctgc ccaagagcct gatccacgca
      541 agcccgttcc atacgagcct gacgatcagt aacctcgcga gcatccgcac gaaccatatc
      601 ttccatcatg tgtacgagtt cggcactacg agcatcctca tcacgctcgg aaacatgcgc
      661 gaagtccccg tacgtaaagg cgaggagatc gtctttgaac gcagcctgcc catgggtgtg
      721 gtcatggacg aacgcatctg ctcgggcagc tactttgccg ccgtgttccg ccgctttaaa
      781 cagtatctga ccaatcccgc tctgctggaa gagccgccca aggtcgtaaa caccgtactt
      841 gggcagggag agtaagaata agatacctta tcggcggcaa cgttcaagcg tttccgccga
      901 ttttttcgtt tcgcacgcgg gaatcttgcc gctaaacatt gcgttcctgc ccggtcactg
      961 ttatcatact ctctaacgaa caattttgga accgccggaa cgaacatttc cccaatcaag
     1021 cggacgttca atctgtgcgg aactttataa tggaggccac tcatgcaaaa gaagattatc
     1081 gctaccaccg ccgcgcccgc cgccatcggc ccgtattccc aggcggtttc cgcaaacggg
     1141 tttttatacc tttccggcat gctcgccatc gacccgcaaa ccggttctct cgttggcgag
     1201 gacgcgcctt ctcaggcaaa acagattctc aaaaacatct ccgcgctgct cgccagcgag
     1261 gggctttcca ttgaaaacgt catcaagacc accgtgtttc tgaccgatct ttctgcattc
     1321 ggcgcggtca acgcggtcta cggcgaaacc ttcgccaaga acccgcccgc gcgctcctgc
     1381 gtggaagtca gccgcctgcc gaaggacgcg ctcgttgaga tcgaatgcgt cgcggtatat
     1441 tgagtcttcg tttttcaccc cacacaccta aggagtaact atcatatgat cgatcttttt
     1501 tctaaaaaac acgtcgtgtt tgacggcgca atgggcacgc tcctgctgga agcgggcctg
     1561 cccgcgggcg cttcgcccga cagttggaat atcacccatc cggacgcgat tttgagcatt
     1621 tataaggcgt atctggaagc gggagcggaa gtgattacca ccaacacctt cggctccaat
     1681 aagccgcgcc agaagcgcgg ctcctatacg ccggcgcagc tcgcggaagc gggcgtccgg
     1741 ctcgcgagac aggccgtgga agcttccggc aaggannnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnntcgcg gaagcgggcg tccggctcgc gagacaggcc gtggaagctt
     1921 ccggcaagga ttgcgtggtt gcgctcgata tcgggcctct gggcgagttt attgagccga
     1981 tgggagactt aaccgaggaa gaagcgattg agctcttccg cgaacccatc gaagcaggcg
     2041 tcgccgccgg cgcggactgt atcgtcatcg aaaccatgtg cgacctcacc gaggcgctct
     2101 gcgccgtcac cgccgccaag cagtacggcg gaggactgcc cgttatctgc acgctctcct
     2161 acgatcccaa cggacggctc atgaccggcg tgacgctgga aaaggtcgtc tctgcgctgg
     2221 aagaagcggg cgttacggcg ctgggctgca actgcggggt cggccccgcg cagctcgtcg
     2281 atctgctgcc gcgctttact gcttgcgcgc atattccgct tgtcatgcag ccaaacgccg
     2341 gtttgccgga atttcgagac ggacagaccg tgtatctcgt cgggccggaa gagtattcga
     2401 gcctgatgaa gacgctttcc gacggcggcg tatggggcct tggcggctgc tgtggcacca
     2461 cgcccgcgca tattcaggcg ctggtcaggg cgattcgctg acccggcctt tcccctgttc
     2521 gtagaaaaaa tcaaagattc ccgccgcggt ggcgcgccgg agagtcagaa aagcagtata
     2581 aaaccgcatg cagaatctgg tgtatatcct ctccgtcgtc ctgccgctgc tcgtcgccct
     2641 gggactgggc ttctatacgc gtaaaaagaa catcctttcg ccgcaggcca ttgaaggcat
     2701 gaaaaccttc gtggtcaaat tcgcgcttcc cgctttgctc ttcgggctgt tctttaccgc
     2761 ggagttcgag tcgagcctgc ttgtgttttc ggcaacgacg ttcgtgcttg gctaccttgg
     2821 gcttggattt ggcatcctcg ttgcaaaacc gtttgtcaag cggagcccca tgctgcagta
     2881 tatgaacgtc ggctgggaag tcggcttgtt gggttactcg ctctttacgc tgctctttgg
     2941 cgcggagaat ctacgttata tggcgctgat ggactttggt caggtgctgt tcgtgttctc
     3001 cggcttcttt acgctgctct atgcgcgcaa cggccaggga ctcaaggaat cgtttaagag
     3061 ccttttgaga aaccccatcc catggtctat gctgcttggc gtggcgtttg ccgcttccgg
     3121 cgcttccaag gcgttggagc cctccggcat tgcgtcgctg atcgtttcca tttgcaattt
     3181 cctctccgcg ccgctgtcct gcctgatctt catcgtcgtg ggatacggca tcgagttcaa
     3241 caaaaagaac atcggaaccg ccgtgatctc cgcaatcctt cgaacgttcg tctgctccat
     3301 cctctgtctt gtgtcgctct ttgtcgtcgg caagttcctc gtgctttcgg aagcgatgcg
     3361 ctgggcggtt atcatgtttt atcttgcccc cgcgccgtac gtgctcacga ttttcgcact
     3421 gaacgatcag gagcgcgcgg atatctccat gtcgcttacc atgcagacca tcgtttcgat
     3481 ttcggcgttt atcctgatcg tgctgtttcg aggctaatac acgaaaaaaa acaaaagctc
     3541 cgtccggcaa acagcccgga cggagttttt tattctctgt tggtttacgc gcgcagcatc
     3601 acgccgccca cggggcggac ggcaaagcga tagcagctgc cctcgcccag cacgcgctca
     3661 atctcggcgc ggtaggcttc tacacgatcc agcggaacca gcgcctgaat ggtgcccgca
     3721 aagccgccgc catgcacgcg ccatgcgcca acgccgttca ggaggcgttc ggagagcgcc
     3781 agcgcgagcg cgaggccgcg ttcgctcggg tcaccagggc aaacgttctg caggtactga
     3841 aagctcgaac gaccgctttc gttcatcagc aggcggaagg cttccgcatc cccgtttttg
     3901 agcgcatcgg cctgcttgac cacgcgctcg ttctccgcaa aaaagtgaat ggcgcgcaga
     3961 atggcgcggt cggaaacggt ctttcgcagc tccggcagag cggcgtaaaa cgcgctctcc
     4021 tccacttccc tgagaacggt cttgccgaaa aaccgcgcaa cagcgcccat ttcggcgggg
     4081 atggaagcat attccggcgt caggtcggcg tggttcccgc cgcagttgac gatgcagata
     4141 gcgtagccga gttcggccaa atcgcgatcg atcttttcaa tgcgcggatg ctccggctct
     4201 tcaaaatcaa tggcgataaa cccgcccacc gcgcaggcaa gctgatccat gaggccgctg
     4261 ggcttgccga agtgtttgtt ttcggcatat ttgctggcaa gcgcctgttc cacgggcggg
     4321 atattgcctt cgttgtacag ttcggaaaaa atg
//
LOCUS       DAOMGY010000042         4331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_20954_length_4331_cov_3.364827, whole genome shotgun sequence.
ACCESSION   DAOMGY010000042 DAOMGY010000000
VERSION     DAOMGY010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20954_length_4331_cov_3.364827"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            436..1755
                     /locus_tag="PLR57_01220"
     CDS             436..1755
                     /locus_tag="PLR57_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQQ40103.1"
                     /translation="MRQKSLKHFLFLLVLTFLAVALLACKETGGSTTESSTAQTDAAS
                     QLSFRITWTDYSGRGQAIQKIVDSYNAISADDEDVVMIGGDENQTTIEQMLASNPETV
                     FVLPYRYVQYFGYRGELEDLTSLFTEEKNLFYEKIWQLGTADGKVYGIPWLGHSMCLL
                     YNKTLLEQAGVDAGSITSLDTLVLALEQVGQATNAQGIGLVGAESNDVSWMVNQFIYG
                     FGGSLVDESGKSVVINSPQSADALNFYKNVLGPHAQPTWVDDTGVEVMKCFLNQEIAF
                     EIQGIWGITDIQKNGKPFDVGVIPLSRIGLNAEVGPMMLALPAGISEQGKEQAISFIR
                     YMISMDAQQAILNGEYSPEHDEYYPFRTPIRLDMADTTMMRMHPDYQLFIEGFANPSV
                     DVPVPKWQAVKDDYYQAGLHRVMTGETTIEAFLRWIETEGNRILAEP"
     gene            1832..2905
                     /locus_tag="PLR57_01225"
     CDS             1832..2905
                     /locus_tag="PLR57_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQQ40104.1"
                     /translation="MKILIADDSKTDVAIISSILSDFELLVAYDGVETLERIEQSPDI
                     DVLILDINMPRMNGFEVLEHLKSEPEHQNIAVLILTNFDEIDNEMRGLDLGAVDYIRK
                     PLNIRSLRKRVELQIKLKNARDELAQQNAMLEKRVAERTRESVLTRDVTIHALVSLLE
                     VRNIESSNHTKRTQLMMRALCNHLRAKPPYDILLTESYSTELFDTTPLHDIGKVGIPD
                     HILLKPGKLTPEEFEIMKKHTTYGADALRCMGKESDSLSFIRTAAEIAGTHHEKFDGS
                     GYPAGMKGKEIPFSGRLMAIIDVYDALVNKRVYKPAFSYKEAKEIMLAERGRHFDPEL
                     LDAFFEIESEVMAIADSFGQQTA"
     assembly_gap    2949..2958
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2959..>4331
                     /locus_tag="PLR57_01230"
     CDS             <2959..>4331
                     /locus_tag="PLR57_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQQ40105.1"
                     /translation="LAVLLLILSCPLPSSQAHDDTTKPMIELTQEEQAFIQEHPVIRL
                     GVDPNFVPYEFFDTDGVYKGIAADYIALISVKTGLTMQAQEGLTWTEAYEKAVRGELD
                     VLPCVAQTAERERYFLFSDTYFTFQRAVFINEETEGLNEFKDLFGKTVAVQMNSSHHS
                     YLTQFTEIKLSLYPTVEAGLRAVSNGTEIAFIGNMATSSYLARENGITNLKYFTIDPA
                     AGDPSQTLHFAVRKDWPELVSILNKAIASITKEERTAINNKWVDVEGTLDYQDVLRGV
                     GVGVGILAIVVAVSYLWIIRLRKEVAKRKKAQEELLLAKEEAEQANQVKSLFLARMSH
                     EIRTPLNAIMGMAYLMKKTDLTATQNSYLDKLSQAARTMLGTINDILDFSKIEAGKIT
                     IERISFDLDKLLQKIINIESVKVEEQGIELLLEKDPALPSYFFGDPLRLEQILLNLVN
                     NAVKFTE"
BASE COUNT         1089 a         1071 c         1201 g          960 t
ORIGIN      
        1 ggcgaagggg ttgactggga ggaactgagt gtcatttcgc ttgaacctgc ggcttgagca
       61 ggcgccctgc cgcagccagc ggcgagcaga cagagcgtta gtgcagcaat ggcgaggcgg
      121 cgcagattca tgtttcgtcg ctccatttcg tcgttttagg ataagaaacg ttccgcagag
      181 taccacattt ataaggcttt tcaagtgttt gttctactta attgtatgct tatttccact
      241 gtcgcgtcaa ttgcagcgtg cctttcacaa ctgcaactca tcccgttttt cagcagtaat
      301 tgagaatttg ttgattcaat cgatcgtttg ttgtactatg atattgttaa agtataacca
      361 ttcgtaccga catttcacca ttttttattg ttcgtcatcc aaaatcgtac cgacaagcat
      421 gactgagata tgaagatgag acagaaatct ctgaaacact tcttattctt attggtattg
      481 acgtttttag ccgtcgcgtt gttggcgtgc aaagaaacgg gcggctcgac aaccgaatct
      541 tccacggcac agacggacgc tgcgtcgcag ctgtcgtttc ggattacctg gacggattat
      601 tccggacgcg ggcaggcgat tcagaagatc gtggacagct acaatgccat atccgcggac
      661 gatgaagacg ttgtcatgat cggcggggac gagaaccaaa cgacgatcga gcagatgctg
      721 gcgtccaacc ctgagactgt ttttgtcctg ccgtaccggt acgttcagta ctttggctac
      781 cgcggggaac tcgaggattt gaccagtttg tttacggaag aaaagaacct gttctacgag
      841 aaaatctggc agttgggcac ggcggacggc aaggtgtacg gtatcccgtg gctggggcac
      901 tccatgtgcc tgctctacaa caaaaccttg ctggaacaag caggcgtgga cgcaggctcc
      961 attacaagcc ttgacacgct ggttctggcg ctcgaacagg tgggacaggc tacaaacgcg
     1021 cagggaatcg ggcttgtcgg cgcggagagc aacgacgttt cctggatggt caaccagttt
     1081 atctacggct ttggcgggag tctggtcgat gaaagcggaa agtccgtcgt tataaatagt
     1141 ccgcagtccg ccgacgcgct gaatttctat aaaaacgtcc tcggcccgca tgcgcagccg
     1201 acctgggtag acgacacggg cgttgaggtt atgaagtgtt ttctcaatca ggagattgca
     1261 tttgaaatac agggcatttg gggtattacg gatattcaaa agaacggcaa accgttcgac
     1321 gtgggcgtga ttccgctgag ccggatcggc ctgaacgcgg aagtcggccc catgatgctt
     1381 gcgcttccgg cgggcatcag cgagcagggc aaagagcagg cgatttcgtt catccgctac
     1441 atgatctcca tggacgcgca gcaggcgatt ctcaacggcg aatacagccc cgaacacgac
     1501 gaatattatc cgttccgcac gccgattcgt cttgacatgg ccgatacgac gatgatgcgg
     1561 atgcatcccg actatcagct atttatcgaa gggttcgcaa acccgagcgt ggatgtgcca
     1621 gttccaaaat ggcaggcggt aaaggacgac tactatcaag cgggattgca ccgcgtgatg
     1681 accggcgaaa cgaccattga agcgttcttg cggtggattg aaacggaagg caaccgcatt
     1741 ttggccgagc cctgacgata ccgagaaaga gacgttgttt ctgtacgatt gaaaagagat
     1801 cgaccagctt caacaaaaag gagtctgcct tatgaaaatc ttaattgccg acgattccaa
     1861 aacagatgtc gccattatca gcagcattct ttccgatttt gagttgctgg ttgcgtacga
     1921 cggcgtggaa acgttggaac gcattgaaca aagcccggat atcgacgttt tgattctcga
     1981 tatcaacatg ccgcgcatga acggctttga agtgttggag cacttaaaaa gcgaaccgga
     2041 gcaccagaac attgcggtgc tgatactgac gaatttcgac gagatcgaca acgagatgcg
     2101 ggggcttgat ctgggagcgg tggactatat tcgtaaaccg ctcaacattc gctcccttcg
     2161 caaacgggtc gaactgcaga tcaagcttaa aaacgcccgg gacgagctgg cacagcaaaa
     2221 tgccatgctg gaaaagcgcg ttgcggagcg cacgcgggaa agcgtgctga cgcgggacgt
     2281 caccattcac gctttagtca gcctgctgga agtcagaaac atcgaatcca gcaaccatac
     2341 caagcgcacc cagctgatga tgcgcgcgct ttgcaaccat ctgcgcgcaa agccgccgta
     2401 cgatattctg ctgaccgaga gttacagcac ggagctattc gacactacgc cactgcacga
     2461 tatcggcaag gttggcatac cggaccatat cttactcaaa cccggcaagc tcacgccgga
     2521 agagtttgaa ataatgaaaa agcatacaac ctatggcgcg gacgcgctcc gctgcatggg
     2581 gaaagagagc gacagtcttt cgtttattcg aaccgccgct gaaattgcgg gaacgcacca
     2641 cgaaaagttt gacggctccg gttatcccgc cggaatgaaa ggaaaagaga ttccgttttc
     2701 cggccggctc atggccatta tcgacgtgta tgacgcgctg gtcaacaaac gcgtctataa
     2761 gcctgcgttc tcttacaagg aagcaaagga aatcatgctt gccgagcgcg gcaggcactt
     2821 tgacccggaa cttctggatg cgttcttcga gattgaatcg gaagtcatgg cgattgcgga
     2881 cagctttggc cagcagacgg cctgacggga gggaagcgca tgaaacgggt aaaatggttt
     2941 actgcaacnn nnnnnnnncg ctcgccgttt tgctgctaat cctctcctgt ccgttgccgt
     3001 cctcgcaggc acacgacgat accacgaagc cgatgatcga gctgacgcag gaggagcagg
     3061 cgtttattca ggaacatcct gtgattcgcc tcggcgttga tccaaacttt gtgccgtatg
     3121 agtttttcga tacggacggg gtgtataagg gcattgcggc ggactatatt gcgctgatca
     3181 gcgtgaagac cgggctgaca atgcaggcgc aggaaggact gacctggacc gaagcgtatg
     3241 aaaaggccgt gcggggcgag ttggacgttt tgccgtgcgt ggcgcagacc gccgaacggg
     3301 agcggtattt tttattttcg gatacctatt ttacctttca gcgcgcggtg tttatcaatg
     3361 aggaaaccga aggcctcaac gagttcaaag atctattcgg caaaaccgta gccgtgcaga
     3421 tgaacagttc gcaccacagc tacttgacac agtttacgga aatcaagctg agcctgtacc
     3481 caaccgtgga agccgggctg cgcgcggtct caaacggtac ggagatcgcg tttatcggca
     3541 acatggcgac ttccagttac cttgcgcggg aaaacggtat tacaaacctc aaatatttta
     3601 ccattgaccc cgcggcagga gacccttcac agacgcttca ttttgcggtg cgcaaggact
     3661 ggccggagct tgtgagcatc ctgaacaagg cgattgccag tattactaag gaagaaagaa
     3721 ccgcaatcaa caataaatgg gtcgatgtgg aaggcacgct ggattatcag gacgtactgc
     3781 gcggcgtcgg cgtcggcgtc gggattttgg cgatcgtcgt agccgtttcg tacctttgga
     3841 ttatccggtt gagaaaagaa gtcgccaagc gtaaaaaagc gcaggaagaa ctgcttcttg
     3901 caaaagagga agcggagcag gccaatcagg tgaaatcgct gtttcttgcg cgcatgtccc
     3961 acgaaatccg tacgccgctc aacgcgataa tgggcatggc gtacctgatg aaaaagacgg
     4021 acttgacggc aacccagaac agctacctcg ataaactctc gcaggccgcg cgcacgatgc
     4081 ttggaacgat caacgacatt ctcgattttt ccaagatcga agcgggcaag atcacgatcg
     4141 agcggatttc gtttgatctg gataaactgc ttcagaagat aatcaatatc gagtcggtca
     4201 aggtggaaga acagggcatt gagctgctgc tggaaaaaga ccccgcgttg ccttcctact
     4261 tctttggcga cccactgcgg ctggaacaga ttctgctcaa ccttgtcaac aacgccgtca
     4321 agtttacgga g
//
LOCUS       DAOMGY010000043         4299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_21192_length_4299_cov_2.951461, whole genome shotgun sequence.
ACCESSION   DAOMGY010000043 DAOMGY010000000
VERSION     DAOMGY010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21192_length_4299_cov_2.951461"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PLR57_01235"
     CDS             <1..1153
                     /locus_tag="PLR57_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HQQ40106.1"
                     /translation="AALGEAKYAPQNSVVYEVKRYGGKMARAFDTIGNAERVELLREA
                     TIAARGVSEEISQVQARYIDVDQRVAIVSTDGVFVFDERPRTRVFVQAVAMQNGEAQT
                     GYDSPGSGRGFEAYRETVDVAEAGRVAGNMAVTMLHAPDCPAGYLPVVIDGGFGGVIF
                     HEACGHSLEATAVGRNNSVFCGKLGTKIAADCVSAVDDGTMPGEWGSISVDDEGTPGQ
                     RNLLIENGILKGYLIDKLGSRRMGGMPVTGSSRRQGYTFAPTSRMTNTFICPGTDDEE
                     EMIATMEEGLFAKKMGGGSVNPPTGEFNFVVEEGYWVKHGKIVSPVRGATLIGKGSEV
                     LQKIDRVGRTMWMAPGMCGSLSGSVPTNVGQPRLRVSGITIGGRGGAIE"
     gene            1150..2481
                     /locus_tag="PLR57_01240"
     CDS             1150..2481
                     /locus_tag="PLR57_01240"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HQQ40107.1"
                     /translation="MTNKEFSTLAAAYAKKAGCENCETFYAGGESFEVNANGGEIDRY
                     SVSREAGVSVRVSFQGKQGYAYTERLEEPEKLVDRAIDNAKCIESTDDHPMQKRCEYR
                     QVSREPSALAKLSEQERIALAKRLEQAALEADSRVKRVVYCIVNYDSGSVRIENTQGL
                     SAEKNTDVSVIYVMPSVQEGDEVQTGIAFRMGADATDVEGCAREAVQDALGKLGGTPV
                     ASGFYRVLFRPYAFGDLLTAFSSIFSADEAQKGRSLLANKEGETIASSIVTLTDDPFD
                     PVAPRAFDSEGTPCKVKTVIENGVLKTLLHNLKTAAKAGVESTGNASRASAASPVGVA
                     PSVLRIEPGSLSADELIQTLGDGLIITELEGIHAGVDAISGDFSLKSAGFLAEGGRIV
                     RPVSNITVAGNFITLLKGVSALGSDILFSLPQIGYFASPSVLVERLSVAGS"
     gene            2639..2800
                     /gene="rpmG"
                     /locus_tag="PLR57_01245"
     CDS             2639..2800
                     /gene="rpmG"
                     /locus_tag="PLR57_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQQ40108.1"
                     /translation="MAADARVKVTLSCTECKQRNYNTFKNKKNDPDRLEMKKYCRFCR
                     KHTVHRESK"
     gene            2822..3472
                     /gene="secE"
                     /locus_tag="PLR57_01250"
     CDS             2822..3472
                     /gene="secE"
                     /locus_tag="PLR57_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HQQ40109.1"
                     /translation="MAQDISKLNNGGKSAPEKKQVAKIDAHAHKKLLWSNISKKFLLP
                     ASILFLAAGLFLTVFYVLQLLQVIPQSEAMTLGMKISSIVLGVFLIVTGVFGLMKKHK
                     KLTMAFDVMALVYAIILLTATVKAGAVAIASGITVIVLGIFFAIAVDGMFDLGLIRFF
                     REMNGEVKKLTWLTRKELASHTLAVLVFVLAMAALIYLLDLAFSSGFSAIGSINLG"
     gene            3479..4063
                     /locus_tag="PLR57_01255"
     CDS             3479..4063
                     /locus_tag="PLR57_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HQQ40110.1"
                     /translation="MSVQEAKWYVVHTYSGYENKVKEDLEKSVENAGLQDIILEVKYP
                     TEETIEILPNGKRKVTQRKVFPGYVMVKMLVDVVEKERLTEQGGKSVDLVMNPRAWYV
                     VRNTRGVTGFVGPGSKPVPLSDEEVTAMGVERIPIVLNIEVGETVRVINGPLENFLGR
                     VESLDAERQKVKLTVSMFGRETPVELDFVQVQRL"
BASE COUNT          975 a         1147 c         1303 g          874 t
ORIGIN      
        1 tgctgctctg ggagaagcga aatacgcacc tcagaacagc gttgtgtatg aagtcaagcg
       61 ctacggcggc aagatggcac gcgcgttcga taccatcggc aatgccgagc gggtcgagct
      121 gctgcgcgaa gcgacgatcg cagcccgggg cgtttcggaa gagatttctc aagtgcaggc
      181 acgttatatc gatgttgatc agcgcgtagc gatcgtttcc accgacggcg tattcgtatt
      241 tgacgagcgc ccgcgcacgc gggtattcgt gcaggcggtc gccatgcaaa acggtgaagc
      301 gcagaccgga tacgatagtc cgggcagcgg acgcgggttc gaggcctacc gcgaaacggt
      361 ggacgttgcg gaagcgggcc gcgttgcggg caatatggcc gtcaccatgc tgcatgcgcc
      421 ggactgtccg gcaggttatc tgccggtggt gatcgacggc ggctttggcg gggtcatttt
      481 ccacgaagcc tgcgggcact cgctggaggc aacggcagtc gggcgaaaca actccgtgtt
      541 ctgcggaaag ctcggcacca agattgccgc ggactgcgtc agcgccgtgg acgacggaac
      601 gatgcccggc gaatggggct ccatcagcgt ggacgacgaa ggcacgccgg ggcaaagaaa
      661 tctgctgatt gaaaacggca ttctgaaagg gtatttgatc gacaaactcg gttcgcgccg
      721 tatgggcggc atgccggtga ccggctcctc gcggcggcag ggctatacat tcgccccgac
      781 ttcccgcatg acgaacacct tcatctgccc gggaacggac gacgaggaag agatgatcgc
      841 caccatggaa gaaggcctgt tcgccaagaa gatgggcggc ggctcggtga acccgccgac
      901 gggtgaattc aactttgtgg tcgaggaagg ctactgggtc aagcacggca agatcgtttc
      961 cccggtgcgc ggagcgacac tgatcggcaa aggctccgaa gtgctccaga agattgaccg
     1021 cgtcggcaga accatgtgga tggctcccgg catgtgcggt tcgctctcgg gatcggttcc
     1081 cacgaacgtc ggccagccgc gcctcagggt cagcggcatc acaattggcg gcaggggagg
     1141 tgcaatcgaa tgacgaacaa ggaattttcc acgctcgcgg ctgcgtacgc aaagaaagcg
     1201 ggctgcgaga actgcgaaac cttttacgcc ggcggcgagt cttttgaagt aaacgcaaac
     1261 ggcggcgaaa tcgaccgtta cagcgtcagc cgcgaggcgg gcgtttcggt tcgcgtttcg
     1321 tttcagggca agcaggggta cgcttatacg gagcgccttg aagagccgga aaagctcgta
     1381 gaccgcgcca tcgacaacgc aaaatgcatc gaatcgacgg acgatcaccc gatgcagaag
     1441 cgctgcgaat acaggcaggt atcccgcgaa ccttccgctc tggcaaagct cagtgagcag
     1501 gaacgcattg cgctggcaaa gcggctggaa caggccgcgt tggaagcgga cagccgcgtt
     1561 aagcgcgttg tatactgcat tgtaaattat gattcgggca gtgttcgcat agagaatacg
     1621 caggggcttt ctgcggagaa gaataccgac gtctccgtta tttacgttat gccttccgta
     1681 caggaaggcg acgaggtgca gaccggcatt gcgttccgca tgggagcgga tgcaacggac
     1741 gtggaaggct gcgcgcgtga agcggtgcag gatgcgctcg gcaaactcgg cggaacgccg
     1801 gttgcttccg gcttctaccg cgtgctgttc cggccgtatg cgtttggcga tctgctgacc
     1861 gcgttttctt ccattttctc cgcggatgaa gcgcaaaaag gtcgctcgtt gctggcaaac
     1921 aaagagggcg agaccatcgc cagctcgatt gttacgctga cggacgatcc gttcgatcct
     1981 gtggcgccgc gtgcgttcga cagcgaaggc acaccctgca aagtgaaaac cgtcatagaa
     2041 aacggcgtgc tcaaaaccct gctgcataac ctcaagaccg ccgcgaaagc gggtgtagag
     2101 agcaccggca atgccagccg tgccagcgct gcctctccgg tcggcgtagc gcccagcgtg
     2161 ctcagaatcg agcccggcag tctctcggcg gatgaactca ttcaaacgct cggcgacggc
     2221 ctgatcatta ccgagctgga aggcattcat gcgggtgtgg acgcaatctc cggcgacttt
     2281 tcactcaagt ccgcgggttt ccttgcggag ggcggcagga ttgtgcgtcc ggtttccaac
     2341 atcacggtcg cgggcaactt catcacgctg ctgaaaggcg tttccgctct gggaagcgac
     2401 attttgttct cgcttccgca aataggttat tttgcaagcc ccagcgtgct tgtcgagcgg
     2461 ctgagtgttg cgggcagctg aaaaaactct gctcatgata tgcggatttt ccgcagaaaa
     2521 tcaaggaatt tgaaactttt cgttgacatc gccccttcgc tatggtatct tcataaggtg
     2581 ccccgcgaag gggctcgctt tatgatgttt cgaccttatt aatggagggt tttagaccat
     2641 ggcagcagat gcacgagtca aagtcacatt gtcctgcacc gagtgcaagc agaggaacta
     2701 caacacgttc aagaacaaaa agaacgaccc cgaccgtctc gaaatgaaga agtattgccg
     2761 tttctgccgc aagcacactg tgcatcgcga atccaagtaa cgaatacgaa agaggaacga
     2821 tatggcacag gatatcagca aattgaataa cggcgggaag agcgcgccgg agaaaaagca
     2881 ggtcgccaag atcgatgcgc acgcgcacaa gaagcttctc tggagcaaca tttccaagaa
     2941 gttcctgctg cccgcgagca tcctgttcct tgcggcaggc ctgtttctga cagtctttta
     3001 tgtgctgcag ctgctgcagg tgattccgca gtccgaggcc atgacgctgg gcatgaagat
     3061 ttccagcatc gtgctcggcg tattcctcat tgtcaccggc gtgttcggcc tgatgaagaa
     3121 gcacaagaag ctgaccatgg cgttcgacgt gatggcgctt gtctatgcaa tcatcctcct
     3181 gaccgcaacg gtcaaggcag gtgccgtcgc aatcgcttcg ggcattacgg tcatcgtgct
     3241 tggcatcttc tttgccatcg cggtggacgg catgttcgat ctgggactga ttcgcttttt
     3301 ccgcgagatg aacggcgaag tcaaaaagct cacgtggctt acccgcaaag agcttgcttc
     3361 gcatacgctg gccgtgctgg tgttcgttct ggcgatggct gcgctcatct atctgttgga
     3421 tcttgctttc tcttccggct tcagcgcaat cggcagcatc aacttagggt aatttaaaat
     3481 gagcgttcag gaagcaaaat ggtacgtggt tcatacctat tccgggtatg agaacaaggt
     3541 aaaggaagac cttgaaaagt ccgtcgagaa cgccgggctt caggatatta tccttgaggt
     3601 caagtatccc acggaagaaa ccatcgagat tttgcccaac ggtaaacgca aggttaccca
     3661 gagaaaggta tttcccggtt acgtaatggt caaaatgctc gtggacgtgg tcgaaaaaga
     3721 acgtctcacg gagcagggcg gcaaatctgt cgatctcgtt atgaacccgc gcgcatggta
     3781 cgttgtccgc aacacccgcg gcgtgaccgg cttcgttggc ccgggaagca aacccgtgcc
     3841 gctttccgac gaggaagtga ccgcgatggg tgtcgaacgg attccgatcg tgctcaacat
     3901 cgaagtcggc gaaacggttc gtgttatcaa cggcccgttg gagaacttcc ttggccgtgt
     3961 cgagtcgctc gatgcagaac gccagaaggt caagctcacg gtgtccatgt tcggcagaga
     4021 aacacccgtc gaactcgact tcgtgcaggt gcagaggctg taagcgcaag cgcggcttaa
     4081 atcggttcgg tcgtgaaacg cctgtcgaac tcgactttat gcaggtgcag cggctgtagt
     4141 caacagcatt ccgcgatact gcggaaacaa tcatcgtcta tccatcgcgg acggcttgtc
     4201 cgcgtgcaat agaaccgtgg caggggaggc accccgtacc acatattatt tacggaggga
     4261 acagcaatgg ctaagaaaat tgaaggctac atcaagctt
//
LOCUS       DAOMGY010000044         4297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_21201_length_4297_cov_3.130127, whole genome shotgun sequence.
ACCESSION   DAOMGY010000044 DAOMGY010000000
VERSION     DAOMGY010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21201_length_4297_cov_3.130127"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLR57_01260"
     CDS             <1..255
                     /locus_tag="PLR57_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQQ40111.1"
                     /translation="QSLIWFFDEWDAGRTYWGQEINLGLLEGGVGIVTDKNFATLVPQ
                     AVQDKVLAAETAVRDGSIKVSTAIGDTTNGAVTLREAMQP"
     gene            485..>1566
                     /locus_tag="PLR57_01265"
     CDS             485..>1566
                     /locus_tag="PLR57_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ40112.1"
                     /translation="MEQHDVIVQMKGITKVYPNGIAANQDVDFSVRKGEIHALMGENG
                     AGKSTLMKILFGLEQPTEGEIWINGQKATLSSPNAAIAHSIGMVHQHFMLVPSLTVAE
                     NIVLGMVPKKSGLFIDYKKALEITKKSAEQFNLEVNPDAKVADIPVGMKQKVEILKAL
                     IRGAKILILDEPTAVLTTQETAELFRELIHLKERGYTIIFISHKLNEIKQITDRITIM
                     RGGKAVGVFDTDSVTEQEISRLMVGRDVVLDVEKDLPKPTDVVLRVRNLHYTNEWNKK
                     MLAGVSFDVRKGEILGVAGVEGNGQRELVDMLFDLMEPDSGTATVNGKDVIHASQARI
                     RAMGVSLVPEDRMTYGVAEGATIEENL"
     assembly_gap    1567..1666
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1667..1921
                     /locus_tag="PLR57_01270"
     CDS             <1667..1921
                     /locus_tag="PLR57_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40113.1"
                     /translation="DVGATEFIRKKLVDLSRSGAAVLLVSADLNEVMELSDSLIVLYG
                     GKIAAYFEDTSQLDDEMMGEYMLGLKRQSSEEIGRVCHEG"
     gene            1911..3032
                     /locus_tag="PLR57_01275"
     CDS             1911..3032
                     /locus_tag="PLR57_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40114.1"
                     /translation="MRDKRMLGFELLRGAATIALALGVGAIFIFLSSDEPFTALRYLL
                     IGPLVSLKNGVASFNTQGLYTILAAMIPTIFTGLAVCVMFSSNQFNLAGEGVVMVGGF
                     AAALCGIYIHMNTGWHAVLCVAVAAVIGGLIMLIPAVLKVKLGASEMVTSLMLNYVCM
                     YLVLHLLSYNFADRSKGATQTFPFDATAEIPALVRGGSKLSWGFVVALVFVVIIGLFM
                     YRTRWGYAIRMIGVNQKFAQYSGMKVGGTIVLSQVIGGVLSGMGGSIEVLGRYSTFLW
                     KDLPGFGWTGITVAILAKNNPLLVPLAAFFIAYLNKGCQLMATYCDVPSEMIDIIQAA
                     IFLFFAAEQFLSGARQKIVVKNTRADLTKAAASAEGGNE"
     gene            3032..3988
                     /locus_tag="PLR57_01280"
     CDS             3032..3988
                     /locus_tag="PLR57_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40115.1"
                     /translation="MWQQLLDTILKSEFLFSVLRITAPILFAALGAVIAEKAGVTNIG
                     LEGIMMISALFGVLFSYWTQSWLVGVLGAMIIGILLGLLMGFFALKMKTDIILAGIAV
                     NMLGSGGTIFLLYMFTGMRGNTGALVSQNILIPTVNIPLIKDIPILGDVLSGHSVLTY
                     VAFFAVYLCWLVLYKTPLGLNIRAVGENPNAASSVGVSVLKIKYIAIAVSGALAGLGG
                     AFMSMYYSQGWNSGMVAGRGFIALAAQAMGRGEPVGAMLSSLLFGLAQALRTKVSGLQ
                     GVSSYLVSLIPYVVTILGLVLYSASTIKRIKKRKSAESEAAK"
     gene            3985..>4297
                     /locus_tag="PLR57_01285"
     CDS             3985..>4297
                     /locus_tag="PLR57_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HQQ40116.1"
                     /translation="MTKRIPVLLDGDPGHDDAIAWVLANASPLLEIKAVTSVCGNQTI
                     EKTTYNAGRVMALVGLDVPLAAGRKKPLLADAMNAPSVHGVSGLDGPVLPEPKRKPLE
                     LD"
BASE COUNT          876 a         1129 c         1295 g          897 t
ORIGIN      
        1 cagtccctca tttggttctt tgacgaatgg gatgccggca gaacctactg gggtcaggaa
       61 atcaacctcg gcctcctaga aggcggcgtt ggcatcgtga cggacaagaa ctttgccacc
      121 cttgtgccgc aggccgtgca ggacaaggtc ctggccgccg aaaccgcggt gcgcgacggt
      181 tcgattaagg tttccaccgc cattggcgac accaccaacg gcgcggtcac gctgcgcgaa
      241 gctatgcagc cgtaacaaac gagtaaaaga aaacataagc gggggagcga aaaacgctct
      301 cccgctttgc tgtcgagcgg tgttatggca ttgcatttgg cgcgcggttt cattatagtt
      361 aatgcaaggt caaaaattca gaacagacga tttttcatat gtttcagttc atttctgctt
      421 taaaccgtcc ttcgggaggc tttgcggcag gaactcatca aaagttcggg ggaaaatccg
      481 gcttatggag caacacgacg tcatcgtaca aatgaagggc attacgaagg tatacccaaa
      541 cggcatcgcc gcaaatcagg acgtggactt ttccgtcaga aaaggcgaaa tccacgcgct
      601 gatgggcgaa aacggcgcgg gaaaatccac gctgatgaag attctcttcg ggctggagca
      661 gccgacggag ggtgaaatct ggatcaacgg ccagaaggcg acgctttcct ctccgaatgc
      721 cgccattgcg cacagcatcg gcatggtgca tcagcacttc atgctggtgc cgagccttac
      781 ggttgccgag aacatcgttc ttgggatggt tcccaagaag agcggtttgt tcatcgacta
      841 caaaaaagcg ctggagatta cgaaaaagag cgccgagcag tttaatttgg aagtcaaccc
      901 ggacgcaaag gtcgcggata ttcccgtagg catgaagcag aaggtggaga ttctaaaagc
      961 gctgattcgc ggcgcgaaaa tactcattct cgacgagccg acggcggttt taactaccca
     1021 ggagacggcg gagctcttcc gcgaactcat ccatctcaag gagcgcggct ataccatcat
     1081 cttcatctcg cataagctca acgaaatcaa gcagatcacc gaccgcatta cgattatgcg
     1141 cggcggaaaa gccgtgggcg tcttcgatac cgattccgtt accgagcagg agatttcccg
     1201 cctcatggtt ggacgggacg tcgtgctgga cgtggaaaaa gatctgccaa aaccaacgga
     1261 cgtggtgctg cgggtgcgga atctgcacta taccaacgaa tggaacaaaa agatgctggc
     1321 tggcgtttcg ttcgacgttc gaaagggcga aatcctgggc gttgcgggcg tggaaggcaa
     1381 cgggcagcgc gagcttgtgg acatgctctt cgatttgatg gagccggact ccggcacggc
     1441 cacggtcaac ggaaaagacg tgattcacgc gtcgcaggcg cgcatccgcg cgatgggcgt
     1501 ttctcttgtg ccggaagacc gcatgaccta cggggtcgcg gaaggggcga ccattgagga
     1561 aaacctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngacg ttggcgcaac
     1681 cgagttcatc cgcaaaaaac tggtcgatct ttcgcgttcc ggcgcggcgg tgctgctggt
     1741 ttctgccgac ctcaacgagg taatggagct ttcggacagc ctgatcgttc tctacggcgg
     1801 aaagatcgcg gcttattttg aagacacgtc ccagctggac gacgaaatga tgggcgagta
     1861 catgctcggc ctcaaacgcc agagcagcga ggagatcggg agggtttgtc atgagggata
     1921 aacgaatgct gggctttgag ctgctgcggg gcgcggcgac cattgcgctg gcgctgggcg
     1981 ttggagcgat ttttatcttt ttaagttcgg acgagccgtt caccgcgctg aggtacctgc
     2041 tgatcgggcc tttggtctcg ctcaaaaacg gcgttgcgag cttcaatacg caggggctct
     2101 acacgattct tgcggcaatg attccaacca tctttaccgg ccttgccgtc tgcgtgatgt
     2161 tctcgagcaa ccagttcaac ctcgcaggcg aaggcgttgt catggtgggc gggtttgccg
     2221 cggcgctctg cggcatctat atccatatga acaccggctg gcatgcggtg ctctgcgtcg
     2281 cggttgcggc ggtgatcggc gggctgatca tgcttattcc ggcggtgctc aaagtcaagc
     2341 ttggcgcaag cgaaatggtc acgagcttga tgctgaacta tgtctgcatg taccttgtgc
     2401 tgcacctttt aagctacaac tttgccgacc gcagcaaggg cgccacccaa acgttcccgt
     2461 ttgacgccac ggcggaaatt cccgcgctgg tgcgcggcgg cagcaagctc agctggggat
     2521 tcgtggttgc gctggtgttt gttgtgatta tcggtctgtt tatgtaccgc acgcgctggg
     2581 gctacgccat ccgcatgatc ggcgtcaacc agaagtttgc gcagtactcc ggcatgaagg
     2641 tcggcggaac gatcgtgctc tcgcaggtga tcggcggcgt gctctccggc atgggcggca
     2701 gcattgaagt gctgggcagg tactccacct tcctatggaa ggatttgccg ggctttggct
     2761 ggacgggcat tacggtagcc atcctcgcca aaaacaaccc gctgctcgtg ccgctcgcgg
     2821 cgttctttat cgcctacctc aacaagggct gccagctcat ggcaacctat tgcgacgtgc
     2881 cgagcgaaat gatcgatatc attcaggcag ccatcttcct cttctttgcc gcggagcagt
     2941 tcctctccgg cgcgcggcag aagatcgttg tcaaaaacac gcgtgccgac ctcacgaagg
     3001 ccgcggcgag cgcggaagga gggaacgagt aatgtggcag cagctgctgg acacgattct
     3061 gaaatcggag ttcctctttt ccgttctgcg catcacggcg cccatcctgt ttgcggcgct
     3121 cggtgcggtc atcgccgaaa aggcgggggt caccaacatc ggccttgagg gcattatgat
     3181 gatctcggcg ctcttcgggg tgctcttcag ctactggacg cagagctggc tcgtcggcgt
     3241 gctgggcgcg atgatcatag gcatcctgct tgggcttttg atgggcttct ttgcgctcaa
     3301 gatgaaaacg gatattatcc tcgcgggtat cgcggtgaac atgcttggtt cgggcgggac
     3361 gatttttctg ctgtacatgt tcacgggcat gcgcggcaac accggcgcgc tggtgagcca
     3421 gaacatctta atcccaacgg tcaacattcc gctcatcaag gatattccga tccttggcga
     3481 cgttctctcc gggcattcgg tgctgacgta cgttgcgttt tttgcggtgt atctttgctg
     3541 gctggtgctc tataaaacgc cgctcggcct caacatccgc gccgtgggtg aaaatcccaa
     3601 tgcggcttcg agcgtcggcg tgagcgtgct gaaaatcaaa tacatcgcaa ttgcggtttc
     3661 cggcgcgctg gcgggtctcg gcggggcgtt tatgtccatg tattactcgc agggctggaa
     3721 cagcggcatg gtcgcggggc gcgggttcat tgcgctcgca gcgcaggcca tgggacgcgg
     3781 cgaaccggtg ggcgccatgc tgagctcgct attgttcggg ttggcgcagg cgctgagaac
     3841 caaggtcagc gggctgcagg gcgtttcgag ctatctcgtg tcgctgattc cgtacgtggt
     3901 taccattctc ggcctcgtgc tgtattccgc aagcacgatt aagagaatta aaaagcggaa
     3961 gtccgccgaa agcgaggcgg ccaaatgaca aagcgcattc cggtgctgct ggacggcgac
     4021 cccggccatg acgacgccat cgcgtgggtg ctggcaaacg ccagcccgct gctggaaatc
     4081 aaggcggtca cttccgtttg cggcaaccag acgattgaaa agacaacgta taacgcgggg
     4141 cgcgtaatgg cgctggtcgg gctggacgtg ccgcttgccg cggggagaaa gaagccgctc
     4201 ttggcggacg cgatgaacgc gccgtccgtg cacggcgttt ccgggctgga cggcccggtt
     4261 ctgccggaac ccaaacgcaa accgctggaa ctggacg
//
LOCUS       DAOMGY010000045         4292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_21233_length_4292_cov_3.187161, whole genome shotgun sequence.
ACCESSION   DAOMGY010000045 DAOMGY010000000
VERSION     DAOMGY010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21233_length_4292_cov_3.187161"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1608)
                     /locus_tag="PLR57_01290"
     CDS             complement(64..1608)
                     /locus_tag="PLR57_01290"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011443.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HQQ40117.1"
                     /translation="MPPSKRTKANARTAARIGEISDQTHVVLEKFEFDPPPILYNVRT
                     QVSDLNYDTPPILSAAVSVYHAWQTIASGIANVPVLPVTDEEGHLFGMLTPEEIASYD
                     MRLINSNRLDGVPLFNLLACLDGQLLSPQSEQNEFFGDLILALPNHAGLPELPEGCIV
                     ICGNQPDVISAALESEVGCLIVCESEVDRALVEGCKKTCVISTPYDAYAASRLVFLAI
                     PVSRICRQDNLVSFHLNDYVDDVREATLKSRFRSYPILDDSDCVVGTLSRFHLLRPNR
                     KRVVLVDHNELGQSIPGLEQAEIVEIIDHHRLADVQTGAPVYVRNEPVGSTCTIITNM
                     FFERGVMPSKKLAGLLAAAIVSDTIMFKSPTSTAVDRAMAERMAKIAGLSLETLGREI
                     FSISAAGNHKLDDLYFADFKQFQFSGHTLGISQITSADSEEFLSKKDGFLSLMQKELD
                     NRGYDMMLLMLTDVLKEGTLLLAVGDVESVEHAFNVSFKSNTAYLPGVISRKKQIVPA
                     LSLLWG"
     gene            complement(1616..1675)
                     /locus_tag="PLR57_01295"
                     /pseudo
     CDS             complement(1616..1675)
                     /locus_tag="PLR57_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1968..2834)
                     /locus_tag="PLR57_01300"
     CDS             complement(1968..2834)
                     /locus_tag="PLR57_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQQ40118.1"
                     /translation="MSYRLFTDATSDLPVSFAEQYHVTVLPMGFTMDGKDYSYIPGNS
                     EMPIETFFAKMRAGVPVTTAQINTFTFLEAFEPVLKAGEDVLYIGFSSKLSGTVQSAF
                     TAKEELEATFPGRKVNVVDSLSASLGEGLLVYYAARKASSMDLDQLTRWVEENKLKFA
                     HWFTVDDLVYLKRGGRVSGATAAIATILNIKPVMHTDDGGYLTAVSKAQGRKKSLREL
                     FDHMQKTVLEQETDTVFIGHCDAKEDAEYVASLVHGQYPNMLIHIDTIGTSIGAHSGP
                     GTVALFFVASER"
     gene            3217..>4292
                     /locus_tag="PLR57_01305"
     CDS             3217..>4292
                     /locus_tag="PLR57_01305"
                     /inference="COORDINATES: protein motif:HMM:NF018964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HQQ40119.1"
                     /translation="MKKVISVGMATLASILLIFILVFTSIGLVINDQTFINNEFTKLG
                     ISESMGITNSDLVRSMTQLVDYMEGNADDINVEVTLNGEQVQMFDYPQEAEHMADVRT
                     IYTTIASYRDVGVLVMLILFLFAAVVNFRMAPQRLAQGYLSGSFVMLLIFGFLGTWAA
                     LDFSNFWTFFHQALFWNDLWLLDATESRMINMLPEDIFADIVARIGLYAGAVIVVLLV
                     LSILALVLSSAGYKRRRAVALARAKARKAAVEARDKAKAEAKAAAEKEKRIAEKKARM
                     AAAKARKQQEQEAEQKAAERERIAQERREQKRAAAQEEAAEPEKPVRQARAAAEEPKP
                     RRKREDGAKPARERALAGGRRCACG"
BASE COUNT          905 a         1187 c         1293 g          907 t
ORIGIN      
        1 aaaacctcct tgcccggtcg ggccgggcta tgcgccgttt gcaagagcgg atgcgaaaag
       61 cgatcagccc cagagcaggg aaagcgccgg tacgatttgt ttcttccggg aaatcacgcc
      121 gggcaggtac gcggtgttgc ttttgaagga gacgttgaac gcgtgctcta cggattccac
      181 gtcgcccacc gccagaagaa gcgttccttc cttgagcacg tcggtaagca tcagcagcat
      241 catgtcgtaa ccgcggttat ccagttcttt ctgcatcagc gaaaggaacc cgtccttctt
      301 gctcaaaaac tcctcggaat ccgccgatgt gatctgcgaa atgccgagcg tatggccgga
      361 gaactgaaac tgcttaaagt cggcaaagta caaatcatcc agcttgtggt tgcccgcagc
      421 cgaaatggag aagatctccc gtccgagtgt ttccagcgag agccccgcaa tctttgccat
      481 gcgttccgcc atcgcgcggt caaccgccgt gctggtgggg gacttgaaca tgatcgtgtc
      541 ggaaacgatc gccgccgcga gcagtcccgc gagctttttg ctgggcatga ccccgcgctc
      601 gaaaaacatg ttcgtaatga tcgtgcaggt gctgccgacc ggctcgttgc gcacgtagac
      661 cggcgcgccg gtctgcacgt ctgccaaccg gtggtggtcg ataatctcta cgatctccgc
      721 ctgctccagg ccagggatcg actggccgag ctcgttgtga tccaccagca cgacgcgctt
      781 gcggttggga cgcagcaggt ggaagcgcga gagcgtcccc acaacgcagt cggaatcgtc
      841 cagaatcgga tagctgcgga agcgcgattt cagcgtcgcc tcccgcacgt cgtccacata
      901 atcgttcagg tggaaggaaa caagattatc ctgcctgcag atgcgtgaaa cgggaatggc
      961 gagaaacacg agcctgcttg cggcgtacgc gtcgtaaggc gtggagatga cgcaggtttt
     1021 tttacagcct tccacgagcg ccctgtccac ttcggactcg cagacgatca ggcagccgac
     1081 ttcgctttcc agcgccgcgg aaatgacgtc cggctggttg ccgcagatta cgatgcagcc
     1141 ttccggcagt tccggcaggc ccgcgtggtt cggcagagcc agaattaaat caccgaaaaa
     1201 ctcgttttgc tcggactgcg ggctgagcag ctgaccgtcg aggcaggcca gcaggttgaa
     1261 cagcggcacg ccgtcgaggc ggttgctgtt gattaagcgc atgtcgtagg aggcgatttc
     1321 ctccggcgtc agcatgccga agagatgccc ttcctcatcc gtgaccggca aaacgggtac
     1381 gttcgcgatg ccgctggcaa tcgtctgcca ggcgtggtaa acgctcaccg cggcggagag
     1441 gatcggcggg gtatcgtagt tgaggtcgga tacctgcgtg cggacattgt agagaatcgg
     1501 cggcgggtcg aattcgaact tttcgagcac gacgtgtgtc tggtcgctga tttcgccgat
     1561 gcgggcggcg gtacgcgcgt tcgcttttgt tctttttgag ggtggcatac gagattgccg
     1621 caacgatgga gtcggtgtcg gggtttctat gtccggtaat gtagactggt tccattcggc
     1681 ctccaaattg gtcggaaatt cgttcgtttt ctccgcggcg aaacgccgcg cgctgcagaa
     1741 agtacgtctc gaaaaaaacg ataaaaccgt tgccggaaaa aagagccggc aacgggttca
     1801 tttcttctta ttttaaccgt ttctcggttg aatcgcaagt tcaagcttgt aaatacgggg
     1861 gtacgccaaa ggggcagtcg cccctttgga atccccgcat tttatttcag atgatgggct
     1921 ataaaaaaat cgggcgggaa gccgtccgat ttttttatga tactttctta ccgttcgctg
     1981 gcgacaaaga acagcgccac cgtgccgggg ccggagtgcg cgccaatgct cgtgcctatg
     2041 gtgtcgatat gaatcagcat gttcgggtat tgcccgtgca cgagggaggc cacatattcc
     2101 gcgtcctctt ttgcgtcgca gtggccaatg aacacggtgt ccgtttcctg ctcgagaaca
     2161 gttttctgca tatggtcgaa cagctcgcgg agcgactttt tacgcccctg cgccttgctc
     2221 acagcggtga ggtacccgcc gtcgtccgta tgcatgacgg gtttgatgtt cagtatggtg
     2281 gcgatggccg ccgtagcgcc cgaaacgcgc ccgccgcgct tgagatagac caggtcgtcc
     2341 accgtaaacc agtgcgcgaa cttgagcttg ttctcctcga cccaacgggt aagctggtca
     2401 agatccatac ttgacgcctt ccgcgcggcg tagtaaacga gcaggccttc gccgagcgac
     2461 gccgaaagcg agtccacaac gttgactttc cttcccggga acgtggcttc cagctcttcc
     2521 ttggcggtaa acgcggactg cacggtgcct gaaagtttcg aggaaaaacc gatatagagc
     2581 acgtcctcgc ccgctttgag cacgggctcg aacgcttcta aaaaggtaaa cgtattgatc
     2641 tgcgccgtgg tgaccggcac gcccgcgcgc atctttgcaa agaacgtttc aatcggcatt
     2701 tccgagtttc cggggatata gctgtagtct ttgccgtcca tcgtgaatcc catcggcagg
     2761 acggttacgt gatactgctc ggcgaaagaa accggcaggt cgctggttgc gtcggtaaac
     2821 aaacggtaac tcatagcttc gcctcgtttc cgcgcgctgt cggccaacac gctgtattga
     2881 attcgcgagg gggagttttc tgcgaattat agttcattgt atcgtctttc tcggcgcatg
     2941 acaagctatt taccgaactt gtggtttact ttttggaacc acaaaaaacc gatcggtcgt
     3001 ttgttgcaga gaggcgaccc ggaacgagat tgtctgtatg cagaagattt cgcatctttg
     3061 cggggatatg gtataatatc cacgcacaaa aaaccggaag gccggacggc tccggcagtg
     3121 cggcgcgata cgatttcaag aaacggagcg cggagcggaa gcggcggaca accgtcctgc
     3181 cggcgcgccg gatcataagc aggagatgca gaattcatga aaaaagtaat ttccgtcggt
     3241 atggcgaccc tcgcttccat actattgatt tttattctgg tctttacctc gatcgggctt
     3301 gttatcaacg accagacgtt tatcaacaac gagttcacca aactcggcat cagcgagtcg
     3361 atgggcatta ccaactccga cctcgtgcgc tctatgacac agcttgtgga ttatatggag
     3421 ggcaacgcgg acgacatcaa cgtggaggtc accctcaacg gcgagcaggt gcagatgttc
     3481 gactacccgc aggaagccga gcacatggcg gacgtccgca cgatctatac gaccatagcg
     3541 tcgtaccggg acgtcggtgt tctcgttatg ctgattctgt tcctgtttgc ggcggtggta
     3601 aacttccgca tggcgccgca gcggctcgcg caggggtatc tttccggttc gtttgtgatg
     3661 ctgctgatct ttggtttcct cggcacctgg gccgcgctgg acttttcaaa cttctggacg
     3721 ttttttcatc aggcgctgtt ctggaacgac ctatggctgc tggatgcgac cgagagccgc
     3781 atgatcaaca tgctgccgga ggatatcttt gccgatatcg tcgcgcgcat cggcctgtac
     3841 gcgggcgcgg tgatcgtagt tctgctggtg ctgagcatcc tcgcgctggt tctctcctcc
     3901 gccgggtata agcgccgccg cgcggttgcg ctggcgcgcg caaaggcgcg caaagccgcc
     3961 gtggaagcgc gcgataaagc caaggcggaa gcgaaagccg ccgccgaaaa ggaaaagcgg
     4021 attgccgaaa agaaagcgcg tatggccgcc gccaaggcgc gcaaacagca ggagcaagaa
     4081 gccgaacaga aagccgccga gcgtgagcgc attgcgcagg agcgcaggga gcagaagcgc
     4141 gccgccgcgc aggaagaagc cgctgagccg gaaaaaccgg tcaggcaggc gagggcggct
     4201 gccgaagagc cgaaaccgcg ccggaaacgc gaagacggcg caaagcccgc gcgcgagcgc
     4261 gctctcgcag gagggcgaag gtgcgcctgc gg
//
LOCUS       DAOMGY010000046         4258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_21452_length_4258_cov_3.302165, whole genome shotgun sequence.
ACCESSION   DAOMGY010000046 DAOMGY010000000
VERSION     DAOMGY010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21452_length_4258_cov_3.302165"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLR57_01310"
     CDS             complement(<1..340)
                     /locus_tag="PLR57_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HQQ40120.1"
                     /translation="MEPEVNLTNADWRLMDIVWDHEPIESPELCHIAEQKLGWKRTTT
                     YTVLKRLCEKGYLVSELSVVTSLIARREAGRKEGSQLVNRGFKGSLPAFIAAFLEGGD
                     LSSSEAAQIEK"
     gene            complement(525..1349)
                     /locus_tag="PLR57_01315"
     CDS             complement(525..1349)
                     /locus_tag="PLR57_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ40121.1"
                     /translation="MRITVLVENMSYSPELRAEHGLSLYVETEQTKLLFDMGRTDLFL
                     QNAEKLGVKIDQVDLAVVSHGHHDHGGGLGAFLAENKKANVYVHTRAFERHLSLKQGG
                     VLGEIGLNPLLRSSDRIIFVGERSEIAPGMTLFSGVNERTLFSRCNDTLYMVKDDATV
                     ADDFQHEQNLILAENGKLVLFAGCAHCGIVNILRRAEEIAGRRMDAVFGGFHLQSPSL
                     KKSEPPERIRAVGEALASSGSAFYTGHCTGEEAFGQLKAILGDQLTAISTGAGYEI"
     gene            complement(1421..1678)
                     /locus_tag="PLR57_01320"
     CDS             complement(1421..1678)
                     /locus_tag="PLR57_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HQQ40122.1"
                     /translation="MGELTKLPNIAEKLEGQLAEVGITTFDELKRVGSREAWLRILAR
                     DPSACIMRLSALEGAIQGVRWHHLDEETKRSLKEFYYQHKS"
     gene            complement(1698..2243)
                     /locus_tag="PLR57_01325"
     CDS             complement(1698..2243)
                     /locus_tag="PLR57_01325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ40123.1"
                     /translation="MTQAVGEDECVLIKPDESLAGEIESYRAAFLDCGSSMDGTGPLQ
                     RMERAADWLQFNKDAEDPIKKPSFWVVSEQFAYVRVRDGRIVGMIQFRHEFNDFLRNF
                     GGNIGYSVRPDERRKGYATRMLKECLGVCKAFGLERVLVTCIKGNEGSKRTILANGGV
                     YEKTVFCERDNVTLERYWITL"
     gene            complement(2262..2741)
                     /locus_tag="PLR57_01330"
     CDS             complement(2262..2741)
                     /locus_tag="PLR57_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQQ40124.1"
                     /translation="MSVRSTVKAIILDNGKVLLNRCYDEPNGSYYSLPGGGQEKYETL
                     HDALVRECLEETGYTVEPFRFAALMEEICDSAFIRETYPEYAHKMLHFFVCRLASREV
                     KKPTETDSSQIASEWVDLEEVKNLNLLPKAVKTHFAELIDSERAMFLGSEHIPVNHG"
     gene            2941..4188
                     /locus_tag="PLR57_01335"
     CDS             2941..4188
                     /locus_tag="PLR57_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40125.1"
                     /translation="MKKIRYGIGFATGRKSFKKVLGAYIQTWLEAKKKLPPDCEVSLS
                     LFVAYDLDYLSTQSTDYTNLSQEIVDQFDNIIFLGAKNALRSIERLEKGGVFTKTELR
                     SVFGSGYAGKRNAILYAALDNQIDYLLFLDDDEYPLAVTANSEICLWSGQHVMLSHLL
                     EITNADYTNGHHCGYVSPIPQIRFNEQLTEADFQAFIEAISNDIISWESMKRLMESGG
                     VTYASKEVLTQKQKTDVPEIHGCKFISGANLCINLTNPGRTFPFYNPPGARGEDTFLS
                     TLLHERTVRQIPCYAFHDGFSTYKHILDGALPTQLAPITAESRAVVTRFLGACTGWVR
                     YKPLLVYLTRPDDFEQEMTHMQKTLSEVLPKFAAYAEDDGFLAIEGEFLKYRKNVAKH
                     AAQLHLAQSTWKKLLEVIAAEDR"
BASE COUNT          983 a         1175 c         1079 g         1021 t
ORIGIN      
        1 gcttctcaat ctgcgccgct tccgagctgg aaagatcgcc gccttccaaa aacgcggcga
       61 tgaacgcggg cagggagcct ttgaagccac ggttcacaag ctggcttcct tcttttcgcc
      121 cggcctcccg gcgcgcaatc agcgacgtta cgaccgaaag ttcgctgacc agataccctt
      181 tttcgcatag gcgtttgagc acggtataag tggtcgtccg tttccagccg agcttctgtt
      241 ccgctatgtg gcaaagctcc ggcgattcga ttggttcgtg atcccaaacg atatccatga
      301 gccgccaatc cgcgtttgtt aagttgactt ccggttccat agcactctcc tagtctaacg
      361 ttttagacaa ttaagtctaa cattatagac tttaagtgtc aaccatgttt tcttttgttt
      421 ttgcataaaa tgttataaaa atgtgtgttt ctttgctttt gcttgcacag ttcagatagg
      481 taagcgaagc gaatggagca aaacagcgtg aaaaacctga gggactatat ttcgtatcca
      541 gcgcctgtcg agattgccgt cagctgatcg cccagaatcg ctttgagctg accaaacgct
      601 tcctcgccgg tgcagtgccc cgtataaaaa gccgatccgg acgaagcaag cgcttcgccg
      661 acggcgcgga tgcgctccgg cggttcgctt ttcttcaggc tgggactttg caggtgaaat
      721 cctccgaaaa ccgcgtccat gcgcctgccc gcgatttctt cagctcggcg cagtatgttg
      781 acgatgccgc agtgcgcgca gcctgcgaac agcacgagct tgccgttctc cgcaagaatc
      841 aggttttgtt catgctgaaa gtcgtccgca acagtcgcgt cgtcctttac catatacagt
      901 gtgtcgttgc agcgggaaaa cagcgtgcgt tcgtttacgc cggagaacag cgtcatgccg
      961 ggagcaatct ccgaccgctc ccccacaaag atgattcgat cggaggagcg cagcagcggg
     1021 ttcagcccga tttcgccaag cacgccaccc tgctttaacg agagatgccg ttcgaacgcg
     1081 cgggtatgga catagacgtt cgcctttttg ttttccgcga ggaacgcgcc gagcccgccg
     1141 ccgtggtcgt gatgcccgtg agaaacgacg gcaagatcaa cttgatcgat tttaacaccg
     1201 agcttttccg cgttttgcag aaagaggtcg gttcggccca tatcgaacag gagtttcgtt
     1261 tgttcggttt ccacatagag gctcagcccg tgttcggcgc gaagctcagg tgaataggac
     1321 atgttttcaa caagaaccgt tatccgcatt gtatcttcca atctccagtt gcatgaaata
     1381 gcatgagcgt tttttcctgg ctgttgcctg tcgaaacgcg tcagctttta tgctgataat
     1441 agaactcctt gagcgaccgc ttggtttctt catccagatg atgccacctc acgccctgaa
     1501 tcgcgccttc cagcgccgaa aggcgcatga tgcaggcgga aggatcgcgc gcgagaatcc
     1561 ggagccaggc ttccctgctg ccgacgcgct tgagctcgtc aaacgtcgtg atgccgactt
     1621 ccgcaagctg gccttccagc ttttcggcaa tgttcgggag ttttgtcagt tcgcccatac
     1681 gcatttctcc ttatttatca tagcgtaatc cagtaccgtt ccagcgtcac attgtcccgc
     1741 tcgcagaaca cggttttttc gtaaacgcca ccgttggcga gaatggtgcg cttgctgccc
     1801 tcgttcccct tgatgcaggt gacgagcacg cgctccagtc cgaacgcctt gcaaacgccg
     1861 aggcactctt tgagcattcg tgtggcatag ccttttctgc gctcgtcggg acgaacactg
     1921 tagccgatgt taccgccaaa gttccgcaga aagtcgttga actcgtgccg gaactgaatc
     1981 atgccgacga ttcttccgtc gcgaacccga acataggcga actgctcgga tacgacccag
     2041 aaggatggct ttttaatcgg gtcctccgca tctttgttga attgcagcca gtccgccgcg
     2101 cgctccatac gctggagcgg gcctgttccg tccatggagc tgccgcagtc cagaaacgca
     2161 gcgcgatagg attcgatttc gcctgcaagc gattcgtccg gcttaatcag cacgcattcg
     2221 tcttctccga cagcctgtgt catgcggcgc tcctttccta tttaaccatg attgacggga
     2281 atatgctccg agccgagaaa cattgcccgt tcgctatcga tcaactcagc aaaatgcgtt
     2341 ttgaccgctt ttggcagaag gttgaggttt ttgacttctt ccagatcgac ccattcagag
     2401 gcaatctgcg agctgtccgt ttccgtcggt ttcttcactt cccgcgaagc cagccgacaa
     2461 acaaagaaat gcagcatctt atgcgcgtac tccggatagg tctcgcggat gaacgcgctg
     2521 tcgcagattt cttccatcag cgcggcaaac cggaacggtt caactgtgta acccgtttcc
     2581 tcaaggcatt cgcgcacaag cgcgtcgtgc agcgtttcgt atttctcctg cccgccgccc
     2641 ggaagcgaat agtagcttcc gttcggctcg tcatagcagc ggttgagcag caccttgccg
     2701 ttatccagaa tgattgcctt aaccgtgctg cgtacgctca tttttgacct cttccgcatc
     2761 ttctcttaca ataattattt gtgaagaaca cgcctgttta tttcgatgtt tcgatattat
     2821 accacgctca cacgctgatt tccatacgat tccggaagcc gttgattcga caaatcgtat
     2881 atcaaatggt atagtcaaag ggatcaataa ctccaaaaag agaaacgagg aaggagaatc
     2941 atgaagaaaa tacgttacgg aatcgggttt gctacaggaa gaaagagttt taaaaaggtg
     3001 cttggcgctt acattcaaac atggctggaa gcaaagaaaa agctccctcc cgactgcgaa
     3061 gtcagcctga gcctttttgt cgcatatgat ctggattact tgagtacaca gtcaacggat
     3121 tatacaaatc tgagtcagga aatcgtcgat cagtttgaca acatcatctt tttaggcgcg
     3181 aagaacgcgt tgagaagcat cgaacgtctc gaaaagggcg gcgtttttac caagacggaa
     3241 ctgcgcagcg tgttcggttc cggttatgca ggcaagcgca acgcaatcct ctatgccgcg
     3301 ctggataacc aaatcgacta tctgctgttc ctcgacgacg acgaataccc gctggcagtg
     3361 acagccaaca gcgaaatctg tctctggagc ggacagcacg tcatgctctc gcacctgctc
     3421 gaaataacca acgcggatta tacaaacggg caccactgcg ggtatgtttc gccgattccg
     3481 cagatccggt tcaacgagca gctgaccgaa gccgattttc aggcgtttat cgaggcgatc
     3541 agcaacgata tcatcagctg ggaaagcatg aagcgcctga tggagtccgg cggcgtgacc
     3601 tacgcttcca aagaagtgct tacccagaag cagaaaactg acgttccgga gattcacggc
     3661 tgcaaattca tctccggcgc aaacctctgc atcaacttaa caaaccccgg gcggacgttt
     3721 ccgttttaca acccgcccgg cgcgcgcggc gaggatacct tcctctccac cctgctgcac
     3781 gaacgaacgg tgcggcagat tccgtgctac gcgttccacg acggattttc aacgtacaag
     3841 cacattctcg acggtgcgct gccgacgcag ttggcgccga ttaccgcgga atcccgcgcg
     3901 gtggtcacgc ggtttttagg cgcatgcacc ggatgggtac ggtataagcc gcttctggtt
     3961 tatctcacaa ggccggatga ctttgagcag gaaatgacgc atatgcaaaa aacgctctcc
     4021 gaagtgctgc ccaagttcgc ggcatatgcg gaagacgacg gattccttgc catagaaggc
     4081 gagttcttaa agtacaggaa gaatgtagca aaacacgccg cacagttgca tctggcacaa
     4141 tccacctgga aaaagcttct cgaggttatt gccgcggaag atcgttaata tccatcaagc
     4201 taaaacagcg attgaatgca acaaacccgc ctcaaaagag acgggtttgt ttggtttg
//
LOCUS       DAOMGY010000047         4244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_21555_length_4244_cov_2.770828, whole genome shotgun sequence.
ACCESSION   DAOMGY010000047 DAOMGY010000000
VERSION     DAOMGY010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21555_length_4244_cov_2.770828"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..698
                     /locus_tag="PLR57_01340"
     CDS             9..698
                     /locus_tag="PLR57_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HQQ40126.1"
                     /translation="MKRGDPIKVASLLDAVAGGGGLISLPAYLLAGLPPHNAIATNKL
                     SSCIGTVASTGRFIKNKCVDWPTAIPSALLAVLGAIAGANLVLGISDNAIRYVMLVLI
                     PVLAFVVLKKRDLSGAELVPVSRRRQFVVILTAALVVGMYDGFYGPGTGTFLLLAYTQ
                     LARLPLNLAAGNVKIANLSSNVGSLIVFLINGQTILPLGLIAAVFSIAGHFLGAGLLL
                     KNGSKIVKPFI"
     gene            complement(838..1836)
                     /locus_tag="PLR57_01345"
     CDS             complement(838..1836)
                     /locus_tag="PLR57_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQQ40127.1"
                     /translation="MEQQTMGKRIAQLRKEKGYTQEQLAELVGVSAQAVSKWENDVSC
                     PDISILPQLSTVLGVTTDELLGAKPIEPKVVVVDAGSTKKGKDRKFFSWDADIGSKKD
                     GIWFALLVIALGMAFLLQRLNVISIGIWGIVWPAVILGLGVAWTIKRFSFFSLAVAGL
                     GLYFLLFNLGVTAFVLTWGVIWPSLLVLLGLTILYEALVPHKKHWTSCIHAEKKTVNE
                     FNEDGGRVHMECSFAEENRRVISESFAGGDVDVSFGKTELDLTSVKRFLGGATLSVDV
                     AFGNFDLVVPKSVRLNVKSDKSFGSIQTFGEPDMNTQDVLTVNADVSFGSMNIRYR"
     gene            2108..2593
                     /gene="def"
                     /locus_tag="PLR57_01350"
     CDS             2108..2593
                     /gene="def"
                     /locus_tag="PLR57_01350"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQQ40128.1"
                     /translation="MALRTIRTDDDPSLYKICRPVERFDSRLAELIDDLFETMDTAEG
                     AGLAAPQVGVLRRVVVIDAGEGRVELVNPRIIEKEGLQRGYEGCLSFPGKSGYVERAD
                     HVIVEGFDRNGKKHTYDTTGFFARAVQHELDHLDGVVYLTLITDPPEDFLKEQEETEE
                     E"
     gene            2622..3557
                     /gene="fmt"
                     /locus_tag="PLR57_01355"
     CDS             2622..3557
                     /gene="fmt"
                     /locus_tag="PLR57_01355"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQQ40129.1"
                     /translation="MLDMKIAFLGTPEFALPSLDMLYQSGHELGVFTQPDHPVGRHAV
                     LTPPPTKVYALEHGIPVFQFEKIRLPEGVEALKTFAPDLMVTAAFGQILSAENLAIPK
                     YGCINVHGSLLPKYRGAAPIQWAIINGETETGITTMMTDVGMDTGDILLVDRVSIGEN
                     ETAGELFERLSVLGAETLKRTIATLEAGTLVRISQDESQATKCPMLKKEHGKIDFTKT
                     AREVHNLVRGTNPWPGAYALLDVEPLKIHATLLNECDFGSAQMGTLRGDAKSGLFVRC
                     ADSWLEIVELQASGSKRMNAKDFLKGRPLEGKILA"
     gene            3603..>4244
                     /locus_tag="PLR57_01360"
     CDS             3603..>4244
                     /locus_tag="PLR57_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQQ40130.1"
                     /translation="MNFRKQSLDALCDVTDRGAYANLRVKQLSRNLPQRDAKQAAALV
                     YTTLDHLLTIDYYLSHFVQGAQKPVVRGILRLGVCELLYFDTPAHAAVSESVQLVREA
                     GKPALAGFVNAVLRRVDREREQLPALPKEPLKRLSMQYSYPEWIVAEWLSSYGEAETE
                     ALLSRPTAKIEVRAQSPFSTAELVNALPVESTFNDLDENSVALEKGFDFASDPL"
BASE COUNT          941 a         1208 c         1123 g          972 t
ORIGIN      
        1 tcaatgccat aaaaaggggt gaccctataa aggtggcaag cctcctggac gcggttgcgg
       61 gcggcggcgg gctgatctcg ctacccgcgt acctgcttgc ggggctgccg ccgcacaatg
      121 cgattgccac caacaagctc tcgagctgca tcggcaccgt cgcttccaca ggccggttta
      181 tcaagaacaa gtgcgtcgat tggccgactg caattccttc cgcgctgctg gcggttcttg
      241 gtgctattgc aggcgcgaac ctcgtgctcg gcatcagcga caacgcaatc cggtacgtca
      301 tgctcgtgct catccccgta ctggcgttcg tcgtgctcaa aaagcgcgac ctctccggcg
      361 cggagctagt tcccgtgagc cgcagacggc agtttgtggt gatactcaca gctgcacttg
      421 tggtcggcat gtacgacggg ttctacggcc ccggtacggg aacgtttctg ctgctggcgt
      481 atacgcagct tgcgcgcctg ccgctgaacc ttgccgcggg caacgtgaag attgccaacc
      541 tctccagcaa cgtcggcagc ctgatcgtgt ttctcatcaa cggccagaca atcctgccgc
      601 tcgggttgat cgcggcggta ttttcgattg ccggacattt cctcggcgct ggcttgctgc
      661 ttaaaaacgg ctcgaaaatc gtcaagccgt tcatctaagt cgtgctgggg ctgctgttta
      721 tccggctgat ctacgatctg tttctggttt gagcgtaatt acttcttcaa ctcaaaaaag
      781 caaaagaacc gcacggcgtt tgccgtgcgg ttctttgctt gacttcctat tgctttttta
      841 gcggtaacga atgttcatgc tgccgaacga aacgtccgcg ttgacagtca gcacgtcctg
      901 cgtgttcata tccggctctc cgaacgtctg gatagatccg aacgacttat cggacttgac
      961 gttcaggcgc acggacttcg gcaccacgag atcgaaattg ccaaaggcaa catccacgct
     1021 caacgttgcg ccgccgagga accgctttac gctcgtgaga tcgagttccg tcttgccgaa
     1081 ggaaacgtca acatccccgc ccgcaaacga ttccgaaatc actctgcggt tttcttcggc
     1141 aaatgagcat tccatatgca cgcgcccacc gtcttcattg aactcgttga ccgtcttttt
     1201 ctccgcgtga atgcaactcg tccaatgctt cttatgcgga accagcgctt cgtagaggat
     1261 tgtcagaccc agaagaacca gcaggctcgg ccagatcacg ccccaagtca gaacgaacgc
     1321 tgttacgcca agattaaaga gcagaaagta caatccgagt cccgcaacag ccaggctgaa
     1381 gaacgagaac cgtttgatcg tccaggccac gccgagaccg aggatcaccg ccggccacac
     1441 gatgccccaa ataccgattg agatgacgtt aagccgctgc agcagaaacg ccatgcccag
     1501 cgcgatgacg agcagcgcaa accagattcc atctttcttg gatccaatat ccgcgtccca
     1561 gctgaagaac ttcctgtctt ttcctttctt ggtgctgccg gcatcaacga ccaccacttt
     1621 tggttcgatc ggttttgcgc caagcagttc gtccgtcgtg actcccagca cggttgagag
     1681 ctgcggcaag atcgaaatat ccgggcagga gacgtcgttt tcccatttgc tgaccgcctg
     1741 agcggacact ccaacgagtt cggcaagctg ttcctgcgtg tagccttttt cctttcggag
     1801 ctgcgcgatg cgcttgccca ttgtttgctg ttccatattg tttactccct ttcaaaatcg
     1861 tttcacgtga tggcttgatt atgtcatagc gaaaaaccct ttaccatagc gcatttttcg
     1921 agttgcgaat tttgtatcaa ccaatagtag aatatcatac aaccatcgtt ttttctacga
     1981 attcattttt ggttgaatag agagtttctc tcaaccattg gtagaattcg ccagatacgg
     2041 tgcttgcctt tacactttgc attatttcag ttaaaatacc tatggtaatc aaaggaggat
     2101 tttttgcatg gcactgagaa cgattcgtac ggacgatgat cccagtttat ataaaatttg
     2161 ccggcctgta gaacgctttg acagcagact tgccgagctg attgacgacc tgtttgaaac
     2221 aatggacacc gccgagggtg ctggccttgc cgcgccacag gtcggcgtgc tccggcgcgt
     2281 agttgttatc gacgcgggag aaggccgcgt ggaactcgtg aacccgcgta ttatagaaaa
     2341 ggaaggtttg cagcgcggct acgagggttg tctctccttc cccggcaaat ccggctatgt
     2401 tgagcgcgcg gatcacgtaa tcgtggaagg tttcgaccgc aacggcaaaa agcataccta
     2461 cgatacgacg ggattttttg cgcgcgcggt tcagcacgag ctggatcatc tggacggcgt
     2521 tgtgtacctt acgcttatta ccgacccgcc ggaagacttt ttaaaggaac aggaagaaac
     2581 ggaagaggaa taacacgcgt tcccctgccg cttttcagag gatattagat atgaagattg
     2641 cctttctcgg cacgccggaa tttgcgctcc cctcgcttga catgctttat caaagcggtc
     2701 acgagttggg cgtgtttacg cagccggacc atccggtcgg cagacacgct gtgctgaccc
     2761 cgccgccgac gaaagtatat gcgctggaac acggcatccc cgtttttcag tttgaaaaga
     2821 tccgtctgcc ggaaggtgtg gaagcgctca aaacgtttgc gcccgactta atggtgacgg
     2881 cggcgttcgg ccaaattctt tccgcggaaa atctcgccat cccgaaatac ggctgcatca
     2941 acgtccacgg gtcgcttcta cccaagtacc ggggagctgc tcccatccaa tgggcaatca
     3001 tcaacggtga gacggaaaca ggcattacga ccatgatgac ggacgtcgga atggacacgg
     3061 gcgatattct gctcgttgac cgcgtttcaa tcggcgaaaa cgaaacggcg ggcgaacttt
     3121 tcgagcggct ctccgtgctc ggcgcggaaa cgctcaagcg tacgatagcc actcttgaag
     3181 cgggcacgct cgtacgcatc tcgcaggacg aatctcaggc gacgaagtgt cccatgctca
     3241 aaaaggagca tggcaagatc gattttacta agaccgcgcg agaagtccac aaccttgttc
     3301 gcggcacgaa tccctggccg ggcgcttacg cgctgctgga cgtcgaacca ctcaagattc
     3361 acgcaacgct cttgaacgag tgcgattttg gttccgccca gatgggcaca ctcagaggcg
     3421 acgcaaagtc gggtttgttt gtccgatgtg ccgacagctg gctggaaatt gttgaactgc
     3481 aggcttccgg ctccaaacgc atgaacgcaa aagacttttt gaaaggccgt ccgctggaag
     3541 gtaaaatcct tgcgtagcgg atgtttcgcc aacagcgacc gaatgagaag aaaggcactt
     3601 atttgaactt tcgtaagcaa tccctcgacg cgctctgcga cgttaccgac cgtggcgcat
     3661 acgcaaacct gcgcgtcaaa caattgagcc gcaatctgcc gcagcgcgac gcgaagcaag
     3721 ccgccgcgct tgtttatacg acgctggatc atcttttgac catcgattac tatctttcgc
     3781 atttcgtgca gggcgcgcaa aagccggttg tccgcggaat tctccggctt ggcgtctgcg
     3841 aactgctgta ttttgatacc cccgcgcacg ccgccgtttc cgaaagcgtg cagctcgtgc
     3901 gcgaagcggg aaagcctgcg ctcgcgggtt ttgtcaacgc cgttttacgc cgcgtcgacc
     3961 gcgagcggga gcaactcccc gcgttgccga aagagccgct taaacggctc tccatgcagt
     4021 acagctatcc ggaatggatc gttgccgaat ggctgagcag ctatggcgaa gcggaaacgg
     4081 aagcgctgct ttcccgcccc acagccaaga tagaagtccg cgcgcaatcc cccttctcca
     4141 ccgccgaact cgttaacgca ttgcccgtcg aaagcacgtt caacgatttg gacgagaact
     4201 ccgttgcgct ggaaaaagga tttgactttg cctccgaccc gttg
//
LOCUS       DAOMGY010000048         4234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_21636_length_4234_cov_2.642977, whole genome shotgun sequence.
ACCESSION   DAOMGY010000048 DAOMGY010000000
VERSION     DAOMGY010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21636_length_4234_cov_2.642977"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PLR57_01365"
     CDS             complement(<1..764)
                     /locus_tag="PLR57_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40131.1"
                     /translation="MQSSPLILNVVHRPEMLPEYAETIMEAKSGGLEERGELLRESLD
                     IFRLQQEIAHKNGIKTTIQMTYASLFSDETVALAKEHHDRFGDEISLTLLGLPCEQFE
                     EKYHTKDFCIWMFSKEDKIAIVTDVFEKFFERFGFYPESTGSYYLDAWLINYIKAQYP
                     SVKCAVATCFEEGPKAYHTCNNSWYTLMDGGPWNPWIPSRQNTHAPAANAGEDSGVVA
                     IPHLSRDLIACYDGNGSNFGAHPQNVLRGMIYQGGQ"
     gene            complement(980..1969)
                     /locus_tag="PLR57_01370"
     CDS             complement(980..1969)
                     /locus_tag="PLR57_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQQ40132.1"
                     /translation="MKLNKQNLVLSDLASELGLSKSTVSRAISGNGRVSDETRKRVLE
                     YAEMHGFTPNSIASSLARSRTNNIGVVIPSEAFYNEIPFFQECLMGVCEAASKRNYDV
                     LIAPIAESDISVLKRIVSNRKVDGVLLTRSLVVDYPADFLKAAHVPFVTVGTSSDPAI
                     VQVDTDTVAACRTLTKQILVSCTTRIALILGSLDYIVNKDRLEGFSQALQGAGREIEP
                     SLLFTNVTGGKSVKEACVRAAANGAKCILCGDDYICAKVVEQLVASGKTDINVSAFYN
                     SDILRNLPMVSSVVSVDVRSYGVAAGETLIRLLEHEPIEQKTFVPHTILNYRS"
     gene            2368..3507
                     /gene="nagA"
                     /locus_tag="PLR57_01375"
     CDS             2368..3507
                     /gene="nagA"
                     /locus_tag="PLR57_01375"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HQQ40133.1"
                     /translation="MRYENAMVYGPDFRFHKGGFSVEDGRFCNVLSATGESGTDLENA
                     YVIPGLIDIHNHGNSGQDFSDGDAKGLETIARYLAKNGVTSFAPASMTLPEEQLTAAY
                     QNATAFVKRAPDGCSVLRGIHMEGPFFSEKKKGAQNGTYLKKPDIEMFFRLNKAADGL
                     IKIADLAPELPGSIDYIREISKVCTVSIAHTEANYEQAKAAIEAGVTHVTHLFNVMPP
                     FLHRAPGVVGAAAESPNVRAELICDGMHVHESVVRAAFSMFGAERLCLVSDALSCCGM
                     PNGEYMLGGQRIFLEGTVARLVDAPDTLAGSANNLFGIFRLGLSFGIPAEAAVRMVSY
                     NPACVIGAEGEVGSIDNGKRADFLVLGGDFSLQRVFLGGAELRRN"
     gene            3511..4134
                     /locus_tag="PLR57_01380"
     CDS             3511..4134
                     /locus_tag="PLR57_01380"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HQQ40134.1"
                     /translation="MAERPLYRIRTIRPGEPHTGEEFIISHSRPEGAPAPETRGQVVY
                     LPDEGLEVSMWCREEHPRAVYHNPNDPVYTDSCMEAFLNFFPDAPEYGYIGLEMNFNG
                     ASHCSFGTGRHTRKYVVERGLAHPEVLVDRIELEQASWWRVTCLLRQPLLEALYERDC
                     AFLPGHRMRGNFYKCGDHTALPHWGSWAPVERVDFHVPHLFGDIEVI"
BASE COUNT          911 a         1113 c         1261 g          949 t
ORIGIN      
        1 tactgcccgc cctgatagat catcccgcgc agtacgttct gcgggtgggc gccgaaattg
       61 gagccgttgc cgtcgtagca ggcgatcaaa tcccgtgaga gatgcggaat cgccacgacg
      121 ccgctgtcct cccctgcgtt cgccgcgggg gcgtgcgtgt tctggcgcga ggggatccag
      181 gggttccagg gcccgccgtc catgagcgta taccaggagt tgttgcaggt gtggtaggct
      241 ttgggccctt cctcaaagca ggtcgccacc gcgcatttga cggaggggta ctgcgcttta
      301 atatagttga tcagccaagc gtccagataa tacgatcccg tcgattccgg gtagaagcca
      361 aagcgctcaa aaaacttttc gaatacgtcc gttacaatcg ctatcttgtc ctctttggag
      421 aacatccaga tgcagaaatc cttggtgtga tacttttcct caaactgctc acagggcagt
      481 cccagaagcg ttaaggaaat ttcgtcgcca aagcggtcgt ggtgttcctt tgccagcgcc
      541 accgtctcat cggagaagag cgaagcgtag gtcatttgaa ttgtggtttt gatgccgttt
      601 ttatgtgcga tctcctgttg gaggcggaag atatccagcg actcgcgcag gagttcgccg
      661 cgctcttcca gaccgccgga ctttgcctcc ataatggttt cggcatattc cggcagcatt
      721 tcggggcggt ggacaacgtt gagaatcagg gggcttgact gcatttcggt ttcaatcctt
      781 ccgggagtag tgagtttact gcgcaaccga ttgcgcaaag gcggcacgaa gacgggctgt
      841 tggaaaaacc ggctttttac attgtggctt tgtgcaaccg gttgcgcaac tcgcgcctga
      901 aaaagagtca ctgcggtgtt tcggaaaatt ctactgaggt tcatgcttac gtttggaata
      961 agaattggta aatccggttt caggaacggt agttgagaat ggtatgtgga acaaacgtct
     1021 tttgctcgat gggttcgtgc tccagcagac ggatgagggt ttctcccgcg gcgacgccgt
     1081 agcttcggac gtccacgctg acaacggaac tcaccatagg aaggttgcgg agtatatcgc
     1141 tgttatagaa tgcagatacg ttgatgtccg ttttcccgga agcaacgagc tgctcgacaa
     1201 cctttgcgca gatgtagtcg tctccgcaga ggatgcattt tgcgccgttt gctgcggccc
     1261 ggacgcaggc ttccttgacg ctcttgccgc cggtgacgtt ggtaaagagc agggaaggtt
     1321 caatttctcg tccggctccc tgaagagcct gtgaaaaccc ttccagtcga tccttgttga
     1381 cgatgtagtc cagcgaaccg agtatcagcg caatacgggt ggtgcaggaa acgaggatct
     1441 gctttgtcag cgtccggcag gcggcaaccg tgtccgtatc cacctgaacg atggcgggat
     1501 cgctcgacgt gccgaccgtg acgaacggta cgtgcgcggc cttcagaaaa tccgcgggat
     1561 agtcgacaac cagagaacgt gtgagaagga cgccgtccac tttgcggttg gaaacgatcc
     1621 gcttgagaac cgagatgtcg ctttccgcga tgggagcgat cagtacgtcg tagttccgct
     1681 tggacgccgc ttcacagacg cccatcaaac actcctgaaa gaagggaatt tcattataga
     1741 acgcttcgga aggaataaca acaccaatgt tgttcgtgcg cgaacgcgca aggctgctgg
     1801 cgatgctgtt tggcgtgaaa ccgtgcattt ccgcgtactc gagcacgcgc tttctcgttt
     1861 cgtcgctgac gcgcccgttg ccggagatgg cgcgagacac ggtcgatttg gaaagcccaa
     1921 gttccgacgc caggtcgctg agcactaaat tttgtttgtt gagcttcatt ctgtcctgct
     1981 ccgccgatcg gcggcagttt cgattgcgca accgcttgcg cacaatcatc ataacacgcc
     2041 cgccgcatcg cgtcaacaag gaaaaaattg gccggggttg tgagctgaac gctgtgagaa
     2101 ggcgccggac agtaaggcac gcttatcgcg tgttgcgacg ggaaaagtcc agcatccggc
     2161 tgaattcaga agtattgcgg ggcaagttcg gattctacca tctttcgaaa tgaggacgct
     2221 tatcattttc tattttgttt gtaacaaatt aaactatctt gcgcggaact gcttgcggag
     2281 gcattcggca agcggtagca tgagaattga agaactgcat caacagatca agagattttt
     2341 gaatgatttt tagggaggaa gtacgatatg cgctatgaaa acgcgatggt gtacgggccg
     2401 gactttcgct tccacaaagg cgggttttcg gttgaggacg ggcgcttttg caatgtgctg
     2461 agcgcgaccg gcgaatcggg gacagacctg gaaaacgcgt acgtcattcc ggggctgatc
     2521 gatattcaca accacggcaa ttcgggacag gatttttcgg acggcgacgc aaaagggctg
     2581 gagacgattg cgcggtatct ggcgaaaaac ggcgttacct cctttgcgcc cgcctccatg
     2641 accctcccgg aagaacagct taccgccgcg taccagaacg cgactgcgtt cgtcaagcgc
     2701 gcgccggacg gctgctccgt actgcgcggc atccatatgg aagggccgtt cttttcggaa
     2761 aagaaaaagg gcgcgcagaa cggcacgtat ttaaaaaaac cggatatcga gatgttcttt
     2821 cgcctcaaca aggctgcaga tggattgata aagattgccg accttgcgcc ggaactgccc
     2881 ggttcgatcg attatatccg cgaaatcagc aaggtttgca ccgtttctat tgcgcatacg
     2941 gaagcgaact acgagcaggc caaagccgcc atcgaggcgg gagtaacgca tgtgacgcac
     3001 ctgttcaacg tcatgccgcc gtttctgcac cgtgcgccgg gcgttgttgg cgcggcggcg
     3061 gaatcgccga atgtacgcgc ggaactgatt tgcgacggta tgcacgtgca cgaaagcgtt
     3121 gtgcgtgcgg cgttctccat gttcggcgcg gaacgtctct gcctcgtcag cgacgcgctt
     3181 tcctgctgcg gcatgcccaa cggcgaatac atgcttggcg ggcaaaggat ttttctcgaa
     3241 ggaacggttg cgcggctggt tgacgcgccg gatacgctgg cgggttccgc caacaacctg
     3301 ttcggtattt tccgccttgg gctgtcattc ggcattcccg cggaggcggc ggtgcgcatg
     3361 gtaagttaca acccagcctg cgttatcggc gcggaggggg aagtcggttc cattgacaac
     3421 ggcaagcgcg cggactttct cgtgctcggc ggagactttt cgcttcagcg cgtattcctt
     3481 ggcggcgcgg aactgcggag gaactgagct atggcagaac gtccgctcta tcgcatccgc
     3541 accatccgcc cgggagaacc gcacacggga gaagagttca tcatttcgca ttcgcggccg
     3601 gaaggtgcgc ccgcgccgga gacgcgcggt caggttgtct atctgccgga cgagggactg
     3661 gaagtttcca tgtggtgccg cgaggaacac ccgcgcgcgg tctatcacaa tcccaacgac
     3721 cccgtgtata cggacagctg catggaagcg tttctcaact tctttccgga cgcgccggag
     3781 tacggctata tcgggctgga gatgaatttt aacggcgcaa gccactgctc gtttggcaca
     3841 gggcggcaca cacgaaagta tgtcgttgag cgcggccttg cgcacccgga agtgcttgtt
     3901 gaccgcatcg aactggaaca ggcttcctgg tggcgggtga cctgtctgct gcgtcagccg
     3961 cttctggaag cgctctatga gcgagactgc gcgtttttgc ccgggcacag gatgcgcggc
     4021 aacttctata agtgcggcga ccataccgcg ctgccgcact ggggcagctg ggcgccggtc
     4081 gagcgggtgg acttccatgt tccgcacctg ttcggtgata tcgaagtgat ttagctgaaa
     4141 aactgagttt caaacaattg attacaaagg atcaaacaac cgaatgaaag agatcatcct
     4201 gaaaaacata atcgaacaat tcccattttc cggc
//
LOCUS       DAOMGY010000049         4206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_21860_length_4206_cov_3.863888, whole genome shotgun sequence.
ACCESSION   DAOMGY010000049 DAOMGY010000000
VERSION     DAOMGY010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21860_length_4206_cov_3.863888"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1869
                     /gene="uvrC"
                     /locus_tag="PLR57_01385"
     CDS             <1..1869
                     /gene="uvrC"
                     /locus_tag="PLR57_01385"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQQ40135.1"
                     /translation="MNNIIEEKLKLLPSSPGVYKMFNAAGEVIYVGKAISLKNRVRQY
                     FQSSKNHPPKVAAMVRQIEDFEYIRVANETEAFSLESNLIKQFKPKYNILLKDDKHFP
                     YVRVDLKQDFPRLEIVRRVKNDGAKYLGPFLSGLLLRDGLALVREHYPIRQCKKDIAR
                     MIARRERPCLMYHIGKCCAPCSGNVSREEYHRLLDEVLSFLSGKTDDIIRSLTTQMES
                     AAESMDFEHAAHLRDRIRAIESLSEKQAVIATTNTMMDVFALGRLEGTALVFALFVRN
                     GKVIGTEKFRMDADAEESDADILSAFLSQYYAESASFVPEVLLYQDASDMESIAEWLG
                     GLAGRKVTLHRPQRGEKRRLTEMAYRNCLDVLEKDASLQKRAWERGEGALTQLSAILG
                     LETVPSRLECFDNSHIRGRDTVSSMVVFTDGQPDKSAYRRFRIREEAGGNDLIAMKEA
                     LTRRFQKAAENEAGFLPLPDLLIMDGGPAQLQVALDVLDSFSLDFIPTIGLAELSETI
                     YLPGEPEPIALPRNSAPQHLIERLRDEAHRFAISYHRNVHNRNALYSVLDSIPGVGDK
                     RKRALFDTFLTIEAIKSASLDELKAVPLIDARTAESVYGYFHKENADSPAAEQEQE"
     gene            complement(1926..2426)
                     /locus_tag="PLR57_01390"
     CDS             complement(1926..2426)
                     /locus_tag="PLR57_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40136.1"
                     /translation="MQEMNYKVDRQATSLLDITFWSNIVVIVLLIAINAVNFSRAAAG
                     GETLVKIASPELIAIISAVIAIWMGFSCFTAMRVKARLKNVFLRLSETGVSGVSMPEP
                     MNNKKGTAFEVDYKDITGIRATETHITSKSTVSSLRIVCGDREYDVPSPEHMQELMEI
                     LADRIG"
     gene            complement(2566..3081)
                     /locus_tag="PLR57_01395"
     CDS             complement(2566..3081)
                     /locus_tag="PLR57_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40137.1"
                     /translation="MKKRLFATILCCFTLVFFLACRANAERPFTEVTFGMTRDEVEAV
                     EPDFANLTQLEKSRYNWSCNRTFSGEVGLLGCRFIEDKVDRITWLAEPTTKDAQALFD
                     EVVKVTTACYGKPDYKHTDEIEANESIASRSYEWKLKNSTVICNIYELFQTGTCVVLF
                     YHQLNDELVFR"
     gene            complement(3208..>4206)
                     /gene="fusA"
                     /locus_tag="PLR57_01400"
     CDS             complement(3208..>4206)
                     /gene="fusA"
                     /locus_tag="PLR57_01400"
                     /inference="COORDINATES: protein motif:HMM:NF015706.2"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQQ40138.1"
                     /translation="DKLHAGDIGALPKLQYTATGDTLCDAGDPILFAPIDFPKPSISL
                     AVTAKKQGEDDKVISGLNRIKEEDPTILIEKNPETGDVLIMGLGEMHIDVVCGKLKNK
                     TNVEAAISDPRIPYRETIHTMAEAEGKHKKQSGGSGQYGVVQMRFEPAAEGVDYEFVN
                     AIVGGVVPKEFIPAVEKGLKEALVHGVLAGYPMVGIKATLYDGKYHPVDSKEVAFKSA
                     ARLSYKAACVKANPTLIEPIYRYEIFVPSDYVGAISGDLSSRRGRMLGMNPVDGGTVV
                     IAEAPLSEMFKYATDLRSMTQARGSFTSEFVRYEDVPANIAKKIIEQAKKEDEEEE"
BASE COUNT          918 a         1204 c         1113 g          971 t
ORIGIN      
        1 atgaataaca ttatcgaaga aaaattaaaa ctgctcccct cctcgcccgg cgtgtacaag
       61 atgttcaacg ccgcaggcga ggtgatttac gttggaaaag caatttcgct caagaatcgc
      121 gtgcggcagt acttccagtc cagcaagaac cacccgccga aggtcgccgc catggtgcgg
      181 cagatcgagg acttcgaata catccgcgtc gccaacgaga cggaagcctt ttcgctggaa
      241 tcgaacctca tcaagcagtt caaaccgaaa tacaacatcc tgctcaagga cgacaagcac
      301 tttccctacg ttcgcgtcga tctaaagcag gattttcccc gtctggagat cgtccgccgc
      361 gtcaagaacg acggcgcgaa atacctcggc cccttcctct ccggcctgct tttacgggac
      421 gggcttgcgc tggtgcgcga gcactatccg atccgccagt gtaaaaagga tatcgctcgc
      481 atgatcgcgc ggcgggagcg gccttgcctc atgtaccaca tcggtaaatg ctgcgcaccg
      541 tgttcgggaa atgtttcccg cgaggaatat caccgtttgc tcgacgaggt gctgtctttt
      601 ctctccggca agacggacga tatcattcgc tcgctgacca ctcagatgga atccgccgcc
      661 gaaagcatgg actttgagca cgcggcacac ctgcgcgacc gcatccgcgc cattgaaagc
      721 ctcagcgaaa agcaggcggt catcgccacc accaacacca tgatggacgt gttcgcgctt
      781 gggcggctgg aaggcacggc gctggtgttc gcgctgttcg tccgcaacgg caaagtgatt
      841 gggactgaga agttccgcat ggacgcggac gcggaagagt ccgacgcgga tatcctctcg
      901 gcgtttctct cgcagtacta cgcggaatcc gcgtcgttcg tgccggaggt gctgctctat
      961 caggacgctt cggacatgga gtccatcgcc gagtggctcg gtgggcttgc ggggcgaaaa
     1021 gtcactctcc atcgcccgca gcgcggcgaa aaacggcggc tcacagaaat ggcttaccgc
     1081 aactgtctgg acgtgctgga aaaggacgct tccctccaga aacgcgcctg ggagcgcggc
     1141 gaaggcgcgc tgacacagct cagcgccatc ctcggcctcg aaacagttcc ttcaaggctg
     1201 gaatgcttcg ataactcgca tattcggggg cgtgacaccg tttcctccat ggtcgttttt
     1261 accgacggcc agccggataa aagcgcgtac cggagattcc gcatccgcga ggaagcgggc
     1321 ggaaacgatt tgatcgccat gaaggaagcg ctcacgcggc gattccaaaa ggcggcggaa
     1381 aatgaagcgg gatttctccc cctgcccgac ctcttgatca tggacggcgg ccctgcgcag
     1441 ctgcaggttg cgctggacgt gctggattcg ttttcgctcg actttatccc gacaatcggc
     1501 cttgccgagc tcagtgaaac aatctatctg cccggcgagc cggagcccat cgctctgccg
     1561 cggaactccg cgccccagca tctgatcgag cgcctgcggg acgaggcgca ccgtttcgca
     1621 attagctacc accgcaacgt gcataaccgc aacgcgctgt attccgtgct ggacagcatc
     1681 cccggcgtgg gcgacaagcg caagcgcgcc ctgttcgata cgtttttgac catcgaagcg
     1741 atcaaatccg cctctctgga cgagctcaag gcggtgccgc tgatcgacgc ccgcacagcg
     1801 gagtcggtat acgggtattt ccataaagag aatgcagatt ctcccgccgc cgagcaggag
     1861 caggaataac caatacaaaa aaagcgaacc gtacggttcg ctttttttga tagcgtaatt
     1921 ttgcgtcagc cgatgcggtc ggcgagtatt tccatcagct cctgcatgtg ttccggtgag
     1981 ggaacatcgt attcccggtc gccacagacg atccgcaatg aagataccgt gctcttgctc
     2041 gtaatgtgcg tttccgtggc gcgaatcccc gtgatgtcct tatagtcaac ctcaaacgca
     2101 gtgccttttt tattgttcat gggttccggc atggaaacgc ctgagacgcc cgtttccgac
     2161 aagcgaagaa atacgttttt cagccttgcc ttgacgcgca tcgctgtaaa gcagctgaac
     2221 cccatccaaa tggcgataac cgcgctgatg atggcgatga gttccggtga agcgattttt
     2281 accagcgttt caccgcccgc tgccgcacgg ctgaaattga ccgcgttaat cgcaatcagg
     2341 agcacgatta caacgatgtt gctccaaaat gtaatgtcca gaaggctagt cgcctgacgg
     2401 tcgaccttgt agttcatctc ttgcatttac tttctccata atgcgtactt ttacttttgt
     2461 cttcacgcgc tggtcgtttt tatttgaaat ccgtggaaaa atgcccaatc cttctgacgc
     2521 gaggttcaaa ttaatcgaac atcgatctat aaaacatttt caatattacc gaaacacaag
     2581 ctcatcattt aactgatgat aaaataatac cacacaggtt cctgtttgaa acagctcgta
     2641 aatattgcat atcaccgtgg aattttttaa tttccattcg taactgcgcg acgcaatact
     2701 ctcatttgcc tcgatttcat ctgtatgctt ataatcaggt tttccgtaac aggcggtcgt
     2761 caccttaact acctcatcaa acaaagcctg cgcatccttc gtagtaggtt ctgccagcca
     2821 cgtgattctg tcaaccttgt cttcaatgaa gcggcaaccc agtaacccga cttcgccact
     2881 gaatgtgcgg ttgcaggacc aattatatct actcttttct agctgcgtca aattcgcaaa
     2941 gtccggctca actgcctcga cttcgtcgcg tgtcataccg aatgtcactt ccgtaaacgg
     3001 tctttcggca tttgccctgc aagctaaaaa gaaaacgaga gtaaagcaac aaaggatcgt
     3061 cgcgaacagg cgctttttca tgagtgtttc ttctttctgt aaaagtgttg ttttgtacag
     3121 tcctatacac gaaaaaagaa aaagggcgcg gtttgcctga taatcagcaa aacaacacgc
     3181 ccccgtatgg attcagaagg tttaagctta ttcttcctct tcgtcttcct tcttcgcctg
     3241 ctcgatgatc ttcttggcga tgttggcggg cacgtcctca tagcgcacga attcgctcgt
     3301 aaacgagccg cgcgcctgcg tcatgctgcg aaggtcggtt gcatacttga acatttcgga
     3361 aagcggcgct tccgcgatga cgacggttcc gccatccacc gggttcatgc cgagcatgcg
     3421 gccgcggcgg gacgagaggt cgccgctgat ggcgcccacg taatcgctcg gaacgaagat
     3481 ttcgtagcgg tagattggct cgatgagcgt cgggttcgcc ttgacgcagg ccgccttata
     3541 ggagagacgc gccgcgctct tgaacgcgac ttccttggaa tcgacgggat ggtatttgcc
     3601 gtcgtagagc gtagccttga tgccgaccat cgggtagccc gcgagaacgc cgtgcaccag
     3661 cgcttccttg aggccctttt ccacggcggg aatgaattcc ttgggaacca caccgccgac
     3721 gatggcgttt acgaactcgt agtccacgcc ttccgccgcc ggttcaaagc gcatctgaac
     3781 aacgccgtac tggccgctgc cgccggactg ctttttatgc ttgccttccg cttccgccat
     3841 ggtgtggatg gtttcacggt acggaatgcg cgggtcggag attgccgctt ccacgttggt
     3901 cttgttcttg agcttgccgc agaccacgtc gatatgcatt tcgccaagac ccataatcag
     3961 cacgtcgccg gtttccgggt tcttctcgat caggatggtc ggatcctctt ccttgatgcg
     4021 gttgaggccg gagatgacct tatcgtcctc accctgcttc tttgcggtga ccgcaagaga
     4081 aatgctgggc ttggggaaat cgatgggcgc aaacaggatg ggatcgcctg cgtcgcagag
     4141 tgtgtcgccc gttgcggtat actgcagttt cggcagagcg ccgatatcgc ccgcgtgaag
     4201 cttgtc
//
LOCUS       DAOMGY010000050         4173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_22091_length_4173_cov_2.718796, whole genome shotgun sequence.
ACCESSION   DAOMGY010000050 DAOMGY010000000
VERSION     DAOMGY010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22091_length_4173_cov_2.718796"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLR57_01405"
     CDS             complement(<1..420)
                     /locus_tag="PLR57_01405"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HQQ40139.1"
                     /translation="MKKDRPKRKKRWGWLIPVGILGLLIVFALGMLIVDTPNRREIAE
                     MEIGQIDFSSLNDGTYTGSYIGTKGHMRDVTLTVTIENGVITDISIPDEALDEEGNPA
                     ELGEGVTAIGYLTRAAVAQSLGIDAVSGATLTSKTLLK"
     gene            complement(452..757)
                     /locus_tag="PLR57_01410"
     CDS             complement(452..757)
                     /locus_tag="PLR57_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40140.1"
                     /translation="METATTRTAKPKRLCVLVTEYGRQTTNVRLPYFMFKMGMKFGKK
                     AAANGDISDCDDAMAYLEGFDVAELERSLASGEQELPLTLVDVDEPDKNSHVTVTIE"
     gene            complement(837..1421)
                     /locus_tag="PLR57_01415"
     CDS             complement(837..1421)
                     /locus_tag="PLR57_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQQ40141.1"
                     /translation="MDDKKAQIFSVAKKLFSEKGFKDTNVAEITREAGVAVGTFYLYY
                     SSKEKLFFDVFMEENLKLKQQCLDSLDYSASPQTIIEQMLRINMEGFATNPILRQWYQ
                     SNVYQKLEQVFRKENAMDSMTFLYDVFLVLVERWQAEGKMRRDIDSKMIMMLFAAIIN
                     VDTHKDEIGLEFFPELLQIMTSLVLQGLTEHCPD"
     gene            1940..3052
                     /locus_tag="PLR57_01420"
     CDS             1940..3052
                     /locus_tag="PLR57_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQQ40142.1"
                     /translation="MYCKNCGNPFEGRFCPNCGTPADQPQAPSTPAPPAEQAENYAKP
                     VTKRWWFWALIVLTAIVLVLSLTNKDDKKKAVVEEQVSPVESTVTDEQAEESEEPASL
                     LGIGSAVLEAAAGETEEPVGDVTIEEQVVLDEQGVRITVLSLDLDSWFGPTINLLIEN
                     NADKNLTVQARNCSVNGAMMDPYFSCDVAAGKKANGELSFSSTDFSDAGIQTLKSIEL
                     VFYVYETDTYDEVFSSSMVTISTTAPASFEQTFDASGSEVFNEKGIRFVILGLDDSDP
                     VWGSSIHVYAENNSTRNITVQLTDVSVNRYMIDPYFYSALLIGKVAFDRVTFFQTDLD
                     DNHITSIESLEFRIHVYDSETWDTILDSDVISLTFE"
     gene            3304..>4173
                     /locus_tag="PLR57_01425"
     CDS             3304..>4173
                     /locus_tag="PLR57_01425"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40143.1"
                     /translation="MKRNMWKRIFLVVLCGLLMAAPAFSAGAEGESETEQSWQLTGQV
                     GGTTKALCREGSTLYVGSGLHVLALDVSDPKKMSLIGTSPLLPQFVESLVSDGNGKLY
                     ACCGDGGLVILDVSNPAETQILGSLNTLGYTEGVALDGNYAVLADGPLGVQIADISDP
                     ANPALVSSAYSLAYAYAVALQNGIVYAAGGGSGLFTVDLSDPLSPKEAGLLSLDGFQY
                     DVKIANGRLYTAGAWGGVHVLDIASPLSPVKAANAYTPGWAMALAADGNNLLVLDGAD
                     GALLYGVAGVQPVR"
BASE COUNT          926 a         1060 c         1147 g         1040 t
ORIGIN      
        1 tttgagcagt gttttgctgg tcagagtcgc accgctgacg gcgtcgatgc caagcgactg
       61 tgcgacggca gcgcgggtaa ggtatccaat cgcggtaacg ccttctccga gctcggcggg
      121 attcccttct tcgtccagcg cttcatcggg aattgaaata tcggtgatga cgccgttctc
      181 tatggtgacg gtcagcgtca cgtcccgcat gtgccctttt gttccgatat aggaaccggt
      241 gtacgttccg tcgttcaggc tggaaaagtc gatctgcccg atctccatct ccgcaatctc
      301 gcggcggttc ggcgtatcca cgatcagcat tcccagcgca aaaacgatca gcagccccag
      361 aataccgacc gggatcagcc atccccaacg ctttttccgt ttgggtcgat cttttttcat
      421 tgttctttcc tcccgttttc catctgtcgt gttactcaat cgtaacggtt acgtgcgaat
      481 tcttgtccgg ttcgtccacg tccactaatg tgagcgggag ttcctgttcg ccgcttgcca
      541 gagagcgctc cagttcggcg acgtcgaagc cttccagata ggccattgcg tcgtcgcaat
      601 cggagatgtc tccgttggct gcggcctttt tgccgaactt catacccatt ttgaacatga
      661 aatacggcaa gcgtacattt gtggtctgcc tgccgtattc agttacgagc acacacaggc
      721 gtttcggttt tgcagttctg gtcgttgcgg tttccatttc tttctttctc cttatgtttc
      781 atttatgaat gaataaattc gtattcgttc atgccgctgc atgaaaaaag cagcctttag
      841 tccgggcagt gttccgtcag cccctggagc acgagcgagg tcataatctg cagcagttcg
      901 gggaagaact ccagaccgat ttcgtcctta tgggtatcta cgttgatgat ggcggcaaag
      961 agcatcatga tcattttgct gtcgatatcc cgccgcatct tgccttccgc ctgccagcgc
     1021 tccacgagta cgaggaaaac gtcgtagagg aacgtcatgg agtccattgc gttttccttc
     1081 cggaatacct gctcgagctt ctggtaaacg ttgctctggt accattgccg caggatgggg
     1141 ttcgtggcaa agccctccat gttgattcgc agcatctgct caataatggt ctgcggagat
     1201 gcgctgtaat caaggctgtc cagacactgt tgcttgagct tcaggttctc ttccataaag
     1261 acgtcaaaga agagtttctc cttggaagag taatagaggt agaatgtgcc tacggcgacg
     1321 cccgcttccc gtgtgatttc cgcaacattg gtatccttga accctttttc gctgaacagc
     1381 ttctttgcaa cgctgaatat ctgcgctttc ttgtcgtcca tgccgatacc tccatgtgaa
     1441 actgaatgaa taaatcagaa ttcgttcata ttgtactgcc gattttatgt ttgtcaatac
     1501 tttttttgag aatatatgga aaaagctttg ttgctgaaaa agagggacgt gattgaagga
     1561 agttccccca aaatagacag ggcgcttctc acgaagcgcc cttaattcgg tttgtgcggt
     1621 tggaaagaag tttgctggga aaatcggaag aatgacgact gcaccgacca aaccttacga
     1681 tgtatatact agcaggcaat cgtgaacaag gcatcaacga aacgtaacga ttttaagaca
     1741 aattatgtct tcgtaaaaaa cggcgcaatt gattggtctc cacagttgat actacgagcg
     1801 gaagggcggc ctaaccggcg aaaaattcgc ggcaaaacgt catttgcttg tgttgcgaac
     1861 tgctttctgc tattttgatg gtagagcaaa tcttgcgcgg gctgcgcatt aaattggaga
     1921 ttatgagggg gaagaattga tgtactgcaa gaattgcggc aatccgttcg agggcagatt
     1981 ttgcccgaac tgcggcactc cggccgacca gcctcaagcg ccgtctactc ccgcgccgcc
     2041 agcggagcaa gcggagaatt atgccaaacc cgttaccaag cgctggtggt tctgggcgct
     2101 cattgtgctg actgccattg tgctggtgct gtcgctgaca aacaaggatg ataaaaagaa
     2161 agctgtcgtt gaagaacagg tttctcctgt ggaatccacc gttacagatg agcaggcgga
     2221 ggaaagcgaa gagcccgctt ctctgctggg cattggttcc gcggtgctgg aagcggcggc
     2281 gggcgagacg gaagaaccgg tcggcgatgt tacgattgaa gagcaggttg tgctggatga
     2341 gcagggggtg cgcatcaccg tactttcact ggatttggac agctggttcg ggccaacgat
     2401 caacctgctg attgaaaaca atgcggataa aaatctgacg gtgcaggcgc gtaattgctc
     2461 cgtcaacggc gcgatgatgg atccgtattt ctcctgcgac gttgcggcgg gtaaaaaagc
     2521 gaacggcgag ctctcgttca gctctaccga cttttcggac gcgggaatcc agacgctgaa
     2581 gtcgattgaa cttgtgtttt atgtgtatga aacggatacc tatgacgagg tgttcagctc
     2641 ttccatggtg acgatttcta ctaccgctcc cgcgtcgttt gagcagacgt ttgacgccag
     2701 cggcagcgag gtgttcaacg aaaaaggcat tcgctttgtt attcttggct tggatgatag
     2761 cgacccggta tggggcagca gcattcacgt ctatgcggaa aacaacagca cgcgcaacat
     2821 tacggttcag ctgacggacg tttccgttaa ccgatatatg atcgacccgt atttttacag
     2881 cgcgctttta atcggcaaag tcgcgttcga cagggttaca ttcttccaaa ccgaccttga
     2941 cgacaaccac atcacttcta ttgaatcgct ggagttccgg attcacgtat acgactcgga
     3001 aacctgggac acgattctcg attccgacgt gatctcgctt acgtttgaat aagcaggctt
     3061 tggcaaacga tcctattcga aacgctcctt cgggagcgtt ttttcgtacg caattcgcaa
     3121 gggtgtgtga ttctgcaatt ctccaattta ctacggcaat gaactgtgat ataaagaatg
     3181 caagaaacgc aatacagatc aaaagtcctg ttattttgga gatttggtat atcaagcgct
     3241 gacaggtaaa ctcgttacaa ccaaacagca ctgtttttac aagaaaggcc gaggcgtagc
     3301 tttatgaagc gaaacatgtg gaaacggatt ttccttgttg tcctctgcgg actgcttatg
     3361 gccgcgcctg cgttcagcgc cggagcagaa ggggaatcgg aaacggagca aagctggcag
     3421 ctgaccggac aggttggcgg cacgaccaaa gcgctctgcc gcgaagggtc gacgctctac
     3481 gtcggctccg gcctgcacgt gctcgcgctg gacgtaagcg acccgaaaaa gatgagtctg
     3541 atcggtacga gcccgctgct gccgcagttt gtggagagcc tcgtcagcga tggaaacggc
     3601 aaactctacg cctgttgcgg ggacggaggg ctcgtcattc tggacgtttc caatcccgcc
     3661 gaaactcaaa ttctgggttc gctcaacacg cttggctaca cggagggcgt tgcgctggac
     3721 ggaaactacg ccgtacttgc cgacgggccg cttggcgtgc agattgcgga tatttccgac
     3781 ccggcgaatc ccgctcttgt ttcttctgcg tactcgctgg cgtatgctta cgccgtcgcc
     3841 ttacagaatg gaatcgtcta cgcggcgggc ggagggagcg gcctgtttac ggtcgatctc
     3901 agcgacccgc tcagcccgaa agaagcaggg ctgctttcgc tggacgggtt ccaatacgat
     3961 gtgaagatcg ccaacggcag gctttatacg gcaggcgcct ggggcggcgt gcacgtgctg
     4021 gatatcgcta gcccgctgag ccccgttaag gcagccaatg cgtatacgcc cggctgggcg
     4081 atggcgcttg ctgcggacgg caacaacctg cttgttctcg acggcgcgga cggcgcgctg
     4141 ctctacggcg ttgcgggggt tcagccggtg cgg
//
LOCUS       DAOMGY010000051         4005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_23354_length_4005_cov_3.369367, whole genome shotgun sequence.
ACCESSION   DAOMGY010000051 DAOMGY010000000
VERSION     DAOMGY010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23354_length_4005_cov_3.369367"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLR57_01430"
     CDS             <1..667
                     /locus_tag="PLR57_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40144.1"
                     /translation="LETLIDLGLGHLAGVENVRWMKQNSGEYTFEQVTNGRLYTGVPN
                     ARASCVISGSDALDVSYLPIAEAEEYSALYDSFRVRRSPCQTVVGGRVLIYPFGRFAG
                     PQEIPPMLLNDVRQEILQDVLNRSGRLTAPMVEQNPYLVPYAFSSDDKAHLYLVNGSM
                     DAVCGVPLLWANADGEYSVSALPSRGEPIAFTARIERDRCMLPLEIEPMESVLLTVRR
                     VGE"
     gene            702..1748
                     /locus_tag="PLR57_01435"
     CDS             702..1748
                     /locus_tag="PLR57_01435"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HQQ40145.1"
                     /translation="MQAKIIERKWVVEWFDERQCYHMPSLTLADNGNLLAVWNGGFLQ
                     WNGDPMGRDAKDWVSVLEYGKSAWSNPDAVVCDIRYCCHDPIWIKNKKGEITLLFAKF
                     LDTEVNFTTWCNGRDELWMRKTPDGGRTWLPAHPANITSGHASNDSVLLPDGAIVFAS
                     TSSELKDKYFGAIRIYISHDDGETFETGPLLYAEDGNLIREPALCLRPNGVVRLFSRT
                     CPGNVGWGSAGNHSLPSYTCESRDGGRTWTKPLPSGILNNESKIDVISWDGDTILMAY
                     NDTPETDWHERSPLTLAMSRDEGKTWTNILELASAPGNKCQPAMCKDKDGRLNIVYMH
                     RHTAIEHIVVEITK"
     gene            complement(1878..3047)
                     /locus_tag="PLR57_01440"
     CDS             complement(1878..3047)
                     /locus_tag="PLR57_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQQ40146.1"
                     /translation="MIQNLNKFTFAAFGRILSETAPDRGFPQEPGWTTERLCYASEPV
                     WVGRVEGMPVYLDFVRGMSVLAVARQGEELEYFYLDKPVCLNAGVEFAVIPRGECEIL
                     RAQHVKGRIELLYPLDTGVLRLNITNQIEITGIYTLFYQEKEKGFFFKGESHDLLELV
                     YVDKGVLHNVVGGADTVLVQGEMMIYGPGLWHMQYADVDAEVSFITVSFELGGSGYDS
                     ILNRRIRLDTEGVRLLRRMLEARDKNNRYSGDLIICSLQMLLLTCLEADEKAAEPRLK
                     TPASLQSENAVVSSALRYIGANVEQKLSVPLVAKSCSVSPSRLSALFQERLGITPGEY
                     IRRVKLEESKTLIKAGEGNFSEIASRLNYSTVQQFSRQFKAKFGVTPSEFAKSVR"
     gene            complement(3143..>4005)
                     /locus_tag="PLR57_01445"
     CDS             complement(3143..>4005)
                     /locus_tag="PLR57_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQQ40147.1"
                     /translation="EEVVWHPKTTADPRYHFEEIVKAFQTAAEKMPRVDAIGVSSAGV
                     FVGNSPMVSSLFLKVPREQRAFLKDIYNAAARKIGDGNVPIVVANDGDVTALAGAMEL
                     KTGAVMGLAMGTSEAVGYVNELGNILGWFNELAFAPVDLSEDALMDEWSGDIGVGCKY
                     FSQDAVIKLAPAAGITLSDGLTPAEKLKEVQKLLEQGWDAAAGIYRTIGAYLAHTLAL
                     YEKFYDIRHLLVLGRVVSGEGGKLILSACTEVLKAEYPEIANRVSVILPDEQSRRVGQ
                     SVAAASLPVV"
BASE COUNT          848 a         1241 c         1134 g          782 t
ORIGIN      
        1 gctggaaacg ctgatcgatc tcggacttgg ccatctcgcg ggggttgaaa acgtgcgctg
       61 gatgaaacag aacagcggcg aatatacctt cgagcaggtc acaaacggca ggctgtatac
      121 gggcgttccc aacgcgcggg caagctgcgt gatctccggt tcggacgcgc tggacgtttc
      181 gtacctgcca atagccgaag cggaagaata ttccgctctg tacgacagtt tccgcgtccg
      241 caggtcgccc tgccaaaccg tcgtgggcgg gcgagtgctg atctatccgt tcggccgatt
      301 tgccggcccg caggagattc cgccaatgct cttaaacgat gtgcggcagg agattctgca
      361 ggacgtgctg aaccgttccg gccgtcttac cgcgccgatg gtcgagcaga atccgtatct
      421 cgtgccctac gcgttttcca gcgacgataa agcgcatctc tacctcgtca acggttccat
      481 ggacgcggtc tgcggcgttc cccttctttg ggcaaacgcg gacggagaat actccgtctc
      541 agcgctgcca agccgcgggg agcccatcgc ctttaccgca agaatcgagc gggaccgctg
      601 catgctgccg ctcgaaatcg agccaatgga aagcgtgctg ctgaccgtcc gccgcgttgg
      661 tgaatagcaa aacagaattc aagaacatca aaggagtttt tatgcaggca aagatcattg
      721 agcgcaaatg ggttgtggaa tggttcgacg aacggcagtg ctaccacatg ccgagcctca
      781 ccctcgcgga taacggcaat ttgctcgccg tctggaacgg cggttttctc cagtggaacg
      841 gcgacccgat ggggcgcgac gcaaaggact gggtctccgt gctggaatac ggcaaaagcg
      901 cctggtcgaa cccggacgcg gttgtctgcg acatccgcta ctgctgccac gacccgatct
      961 ggatcaagaa caaaaagggc gaaatcacgc tgctgtttgc caagttcctc gatacggaag
     1021 tgaactttac cacctggtgc aacgggcgcg acgagctctg gatgcgcaaa acgcccgacg
     1081 gcggacgcac atggctgccc gcgcacccgg cgaacatcac ctccggccac gccagcaacg
     1141 acagcgtgct tttgccggac ggagcgatcg tctttgccag cacgagcagc gagctcaagg
     1201 acaaatactt tggcgccatc cgtatctata tcagccatga cgacggggaa acgtttgaaa
     1261 cggggccgct cctctacgcc gaggacggga acctgattcg cgaacccgcg ctctgcctgc
     1321 gcccgaacgg ggtcgtccgt ctcttttcgc gcacctgccc cggcaacgtc ggctgggggt
     1381 cggcgggcaa ccattccctg ccctcttaca cctgcgaaag ccgcgacggc gggcgcacct
     1441 ggacaaagcc gttgcccagc ggcatcctca acaacgaatc caagatcgac gtcatcagct
     1501 gggatggcga caccatcctg atggcgtata acgacacgcc ggagacggac tggcacgagc
     1561 gcagcccgct gacgctcgcc atgagccgcg acgagggcaa aacctggaca aacattctgg
     1621 aactcgcttc cgcgcccggc aacaagtgcc agcccgccat gtgcaaggat aaagacggac
     1681 ggctgaacat cgtgtacatg caccgccaca ccgccattga gcatatcgta gttgagatta
     1741 cgaagtaagc gcacacagtg cgtcaaaaag ccccgaagaa cggttgttgt tctttggggc
     1801 tttttttgac gattctcggc tttcagcgaa cccgccgcgg cgctgaaaag cgcatcgtgt
     1861 tcgcgctatt gcccgcttca gcggacggac tttgcaaact cgctcggcgt taccccgaac
     1921 tttgctttga actggcgcga aaactgctga acggttgaat agttgagcct tgacgcgatt
     1981 tctgaaaaat tcccttcgcc cgctttgatg agcgtcttgc tctcctccag cttcacgcgg
     2041 cggatgtact cgccgggcgt aatgccgagc cgctcctgaa acaacgccga aagccgcgat
     2101 ggactgacgc tgcagctttt tgccacgagc ggaacggaga gtttctgctc cacgttcgcg
     2161 ccgatatagc gcagggcgga actgaccacg gcgttttcgc tctggagcga agcgggcgtt
     2221 ttgaggcgcg gttccgccgc tttttcgtcc gcttcgaggc aggtgagcag cagcatctgc
     2281 agcgagcaga tgatcaaatc cccgctgtag cggttgttct tgtcgcgcgc ttccagcatg
     2341 cggcgaagca accgcactcc ttccgtgtcc agacggatac ggcggttcag gatggaatca
     2401 tacccactcc cgcccagctc gaaggaaacg gtaataaagc ttacttccgc atccacatcg
     2461 gcatactgca tgtgccagag ccccgggccg tagatcatca tttcgccctg aaccagcacg
     2521 gtatcggcgc cgccaacgac gttgtgcaga acgcccttat ccacatagac aagttccagc
     2581 aggtcgtgag attcgccctt aaaaaagaag cccttctcct tttcctgata aaagagcgtg
     2641 tagatgccgg tgatttcgat ctgattggtt atgttgagcc gcagaacacc cgtatccagc
     2701 ggatacagca gctcaatacg ccccttgacg tgctgcgcgc gcaaaatttc gcactctccg
     2761 cgcgggataa cggcaaactc cacgcccgcg ttcaggcaga cgggcttgtc gagatagaaa
     2821 tactccagtt cttcgccctg ccgagcgacc gcgagtacgc tcatgccgcg cacaaagtcc
     2881 agatagaccg gcatgccctc cactcttccg acccagacag gttcggaagc atagcaaagg
     2941 cgttcggtcg tccagcccgg ctcctgcgga aagccccggt cgggagctgt ttccgaaaga
     3001 atccggccga acgcggcgaa ggtaaactta ttcagatttt gaatcattcc gccgtcgccc
     3061 tccgctcttc gatatggctc tattataccg aaaaacgggc gattttgcca tgtagaagcg
     3121 caaggaatcc gccgttttga cgttacacaa ccggcaggct cgccgctgcg acggactgcc
     3181 cgacccggcg ggactgttcg tccggcagga tgacggaaac gcggtttgca atctccggat
     3241 actcggcttt cagcacttct gtgcaggcgg agaggatgag ctttccgccc tcgccggaga
     3301 ccacgcgccc cagcaccagc agatgccgga tatcgtagaa cttttcgtaa agcgcgaggg
     3361 tatgcgcaag gtacgcgccg atcgtgcggt agatgcctgc cgccgcgtcc cagccctgtt
     3421 caagcagctt ctgtacttcc ttgagttttt ccgcgggggt caaaccgtcc gaaagggtga
     3481 tgcccgccgc tggcgcgagc ttgatgaccg cgtcctgcga aaaatacttg cagcctactc
     3541 cgatatcgcc cgaccactcg tccattaagg catcctcgct gagatcgacc ggcgcaaacg
     3601 ccagctcgtt gaaccagccg aggatattgc ctagctcatt gacgtaaccc accgcctcgc
     3661 tcgtgcccat ggcaagcccc atgactgcgc cggtcttgag ctccatggcg cccgccagcg
     3721 cggtaacgtc gccgtcgttg gcgaccacga tgggtacgtt cccgtcgcca atcttccggg
     3781 cagcggcgtt gtagatatcc ttgagaaacg cacgctgttc acgcggtact ttcaaaaaca
     3841 gcgacgagac catcgggcta ttgcccacga aaacgcccgc cgacgaaacg ccgatggcgt
     3901 ccacgcgggg catcttttcc gccgccgtct gaaacgcctt gacgatctct tcaaaatgat
     3961 accgcgggtc ggcggtggtc tttgggtgcc agacgacctc ctcgg
//
LOCUS       DAOMGY010000052         3965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_23651_length_3965_cov_3.320205, whole genome shotgun sequence.
ACCESSION   DAOMGY010000052 DAOMGY010000000
VERSION     DAOMGY010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23651_length_3965_cov_3.320205"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..678
                     /locus_tag="PLR57_01450"
     CDS             220..678
                     /locus_tag="PLR57_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQQ40148.1"
                     /translation="MSWNENEDKARTSTLLRQLFKAPDLDSFFDRYEGTLQTPELHNH
                     LIALCKERGLKKDTVIKNAGIERTYGYQIFNGTRKPSRDKVLQIALAMRLNMDETQKL
                     LRVADKSLLYPRLKRDAAVIYCVVHGLGCMSAQNLLDELGLHLLGDETAD"
     gene            732..2504
                     /locus_tag="PLR57_01455"
     CDS             732..2504
                     /locus_tag="PLR57_01455"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ40149.1"
                     /translation="MEMEQSAAREFLLDYEEKSAAYYPEEFLQKYEPIECFANNQMGE
                     TLLVKDHSNRPYIAKCYTDSSLLSKTTEGELLKSLRHSGLPSFIEEIRGQNMICVVHQ
                     YVRGVPLNRLRERLTREQAVALAIQLCGVLRYLHEQTPPLIHRDIKPQNIILGDDGQI
                     TLIDFGISRYYDKNAHSDTVCFGTQEFAPPEQYGFSQTDCRADIFSLGVLLGWMLTGQ
                     TSDFVIPDKRLAHIVKKCTAFSPKDRYTSVAAVRRALLNADGHVEKAIARTATAAVFS
                     LGLLAGGFALGRYTEVRPPLFYQTGPTAIQDSVLESAIRTELNLTSEEALTGDALASV
                     TELYVYGDHTAASSEDFYALRNDVFSGKTVIGTDAVGSLEGLTKLPNLVQLSLSETNA
                     ADLSPLSELTKLQSLELYYSPATDFSPIAALPNLRHLVIQNCNYITDISFLTDCKNVR
                     ELVLTDDGRIADYSALAEMGELQYLHLEGVDPDLFLSYLSGKTIQQLKIGWHSLSSLA
                     ELAQIQGLEELICNGIDFDSLSGGEQLTTLERLSIKAADGQTDMDLSPLLTLPKLKTL
                     TLSENLRQAAETALTDAPFEIKYE"
     gene            complement(2577..2948)
                     /locus_tag="PLR57_01460"
     CDS             complement(2577..2948)
                     /locus_tag="PLR57_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HQQ40150.1"
                     /translation="MQVNKSIEDYLEQILIQQERNGCARSVEIAGNLGVTKASVSHAT
                     KLLRENGFIRMADDKCITLTPAGREIAERMYQRHRVLAQFLMRLGVCEEVAFADACKM
                     EHDISAESFAAICLHADAEWK"
     gene            3287..3658
                     /locus_tag="PLR57_01465"
     CDS             3287..3658
                     /locus_tag="PLR57_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HQQ40151.1"
                     /translation="MTLDEMKVGQTATIRVVGGEGALRLRFLDMGLIPKTKVTLIKTA
                     PLGDPIEICVRGYELTLRKDDAKKIELTDEGGQCDGICAGGKSKLGKNDAVQCAYGVQ
                     SACRKLSGRDGGAEVGGDSRG"
     gene            3567..>3965
                     /locus_tag="PLR57_01470"
     CDS             3567..>3965
                     /locus_tag="PLR57_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HQQ40152.1"
                     /translation="MFNALTGSNQHVGNFPGVTVEQKSGEIRGVKSASVVDLPGIYSI
                     RPYTQEEIVSRDFILDGKPDGIINIVDATNIERNLYLTLQLLELRIPMVVALNMMDEV
                     RANGGSIDIKLMSSLLGVPVVPISASKNEGV"
BASE COUNT          918 a         1075 c         1115 g          857 t
ORIGIN      
        1 catccggaat ttcgtatgat tttttcaaaa accgtcggcg cggagcgccg cggtgtgcta
       61 taatccaaaa aagccacttc tttcaaccgt aaacaacacg tcgtacccgc agtatgcagc
      121 ggtaaaatct ccgattgggt ttccttaacg gaatcaccgc tgcggcggcg cttgcgcaat
      181 gccgcggaac agccagtgaa ccgcctgagg tgacgatcaa tgtcctggaa cgaaaacgaa
      241 gataaagcca gaaccagcac gctgctgcgg cagcttttta aagcacccga tctggattcg
      301 tttttcgacc gttatgaggg cacgctgcag acgccggaac tgcacaacca tctgattgcg
      361 ctctgcaagg aacgcggcct gaaaaaagac accgtcatta aaaacgcggg catcgagcgt
      421 acatacggct atcaaatttt taacggaaca agaaaaccct cccgcgacaa agtgctgcag
      481 attgcgctcg ctatgcgcct caatatggac gaaacgcaaa aactgctgcg ggtcgccgat
      541 aaaagcctgc tctatccgcg cctcaaacgc gatgcggcgg tgatttactg cgttgtgcac
      601 ggactcgggt gcatgagcgc gcagaacctt ctggacgaac tgggtcttca tctgctcggc
      661 gacgaaacgg cggactgatc ccgctttcgg cggcgaaacc ggaacacatc cttcaactgg
      721 agaatgcttg catggaaatg gaacagtcgg ccgcacggga atttttgctg gactacgaag
      781 agaagagcgc ggcgtattac ccggaagagt ttctgcaaaa gtacgaacca atcgagtgct
      841 tcgccaacaa ccaaatgggt gaaacgctcc tcgttaagga ccattccaat cgtccctaca
      901 tcgcaaaatg ctatacggat tcgtcgctgc tctccaagac aacggaaggc gagctgctca
      961 aaagcctgcg ccacagcggg ctgccttcgt ttatagaaga gattcgcgga cagaacatga
     1021 tttgcgtggt gcatcagtat gtgcgcggcg ttccgctcaa ccgcctgcgt gagcggctca
     1081 ctcgcgaaca ggcggtagcg ctcgccatcc agctatgcgg cgtgctgcgc tatctgcacg
     1141 agcagactcc gccgctgatc caccgcgaca tcaagccgca gaatatcatt ctgggcgacg
     1201 acggacaaat tacgctgatc gactttggca tctcccgcta ttacgataaa aacgcgcatt
     1261 cggacacggt gtgcttcggc acgcaggagt ttgcaccgcc cgagcagtac ggtttttcgc
     1321 agaccgactg ccgcgcggat atcttttcgc tcggcgttct gctgggctgg atgcttacgg
     1381 ggcagaccag cgattttgtg ataccggata aacggctcgc gcacatcgta aaaaagtgca
     1441 ccgcgttttc gccgaaggat cgctatacca gcgttgcggc ggtgcgccgc gcgttgctca
     1501 acgcggacgg ccacgtggaa aaagccattg caagaaccgc gacggcagcc gtgttttcgc
     1561 tggggctact tgcaggcggt tttgcgctgg ggcggtatac ggaagtgcgc ccgccgctgt
     1621 tttatcagac gggacccacg gcaatacagg attccgttct ggagagcgcc attcgaacgg
     1681 agctgaatct gacgtctgag gaagcgctga ccggcgacgc gcttgcgagc gtgacggaac
     1741 tctatgtgta cggcgaccat acggccgcga gcagcgaaga cttctacgcg ctgagaaacg
     1801 acgtgttttc cggcaagacg gtcattggaa cggacgccgt cggttcgctg gagggcctga
     1861 cgaaactgcc gaatctggtt cagctttccc tgtcggaaac gaacgcagcc gatttatcgc
     1921 cgctttccga gttaacaaag ctgcagtcgc ttgaactata ttacagcccc gcaacggact
     1981 tttccccgat tgccgcgctc cccaacctgc gccatctggt cattcagaat tgcaattaca
     2041 taacggatat ttcgtttctg accgactgca aaaacgtacg tgagctcgtg ctcactgacg
     2101 acggcaggat agcagattat tccgcgctgg ccgagatggg ggagctgcag tacctgcatt
     2161 tggaaggcgt ggatccggat ctgttcttat cgtatctcag cggcaaaacc atccagcagc
     2221 tcaaaatagg ctggcattcg ctgtcttcgc tggcggaact tgcgcagatt cagggactgg
     2281 aagaactgat ttgcaacgga atcgattttg actcgctgtc cggcggcgaa caactcacca
     2341 cgctggagcg cctgagtata aaagccgcgg acgggcagac ggatatggac ctttcaccgc
     2401 ttttgacact gccaaagctg aagacgctga cgctcagcga gaacctgcgg caggccgccg
     2461 aaaccgcgct tacggacgcg ccgtttgaaa tcaaatacga gtagaaacaa aaacagacgg
     2521 cataaaccgt ctgttttgtt ttcttcttgc aagagcaggt caaccctctt gaacgtttat
     2581 ttccactccg cgtctgcgtg caggcagatg gctgcaaaac tttccgcgct gatgtcgtgt
     2641 tccattttgc aggcgtccgc aaaagcgacc tcctcgcaaa cgcccaggcg catgagaaac
     2701 tgcgccagca cgcggtgacg ctgatacatg cgctccgcga tttcccgccc cgcgggggtg
     2761 agcgtaatgc acttgtcgtc cgccatgcga ataaacccgt tctcgcgcaa aagctttgtg
     2821 gcgtggctga cgctcgcctt ggtcacgccg agattgccgg caatttcaac agagcgcgca
     2881 cagccgtttc gttcctgctg gatgagtatc tgctcgagat aatcctcgat cgacttgttc
     2941 acctgcatac cggtatcctg ctttccagta gtttcctccg tttgaaaata tgtctccgct
     3001 tcgctgtttg cttggttcta tgctatcact gttgtgcggt tcaattcaag ccttcgtctg
     3061 tttttttcat atattgtacg ataatccatt gaaactgcgc cgggataaca ttcggactgg
     3121 aaaaaatatg gtattttgcg gagacagggg attgacaact tgagttagat agtgtaaact
     3181 ccaatcgcaa gttagattag tctaaccgta gatggccaaa cgctttgcgt atatacgcag
     3241 gcgagggcga gcggtcctcg ggcgcccggt tgaaaggtgg atgcttatga cgctggatga
     3301 gatgaaggtc ggccaaacgg caacgattcg cgtggtcggc ggggaaggcg cgctgcggct
     3361 gcggtttctc gatatggggc tgatccccaa gacgaaggtg acgctgatca agaccgcgcc
     3421 gctgggcgat ccaattgaga tctgcgtgcg cgggtatgaa ctgacccttc gcaaggacga
     3481 cgcaaagaaa attgagctga cagacgaggg aggacaatgc gatggtattt gcgctggcgg
     3541 gaaatcaaaa ctcgggaaaa acgacgctgt tcaatgcgct tacggggtcc aatcagcatg
     3601 taggaaactt tccgggcgtg acggtggagc agaagtcggg ggagattcgc ggggttaaaa
     3661 gcgcttccgt tgtcgatttg ccgggcatct actcgattcg cccgtatacg caggaagaga
     3721 tcgtctcgcg cgatttcatt ctcgacggca agccggacgg cattatcaac atcgtggacg
     3781 cgacgaatat cgagcggaac ctctacctga cgctgcagct gcttgagctg cgcattccaa
     3841 tggtggttgc gctcaacatg atggacgagg tgcgcgccaa cggcggctcc atcgatatca
     3901 aactcatgag ttcgctgctc ggcgtgccgg ttgtgccgat ctccgcttcc aagaacgaag
     3961 gcgtt
//
LOCUS       DAOMGY010000053         3928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_23987_length_3928_cov_2.442809, whole genome shotgun sequence.
ACCESSION   DAOMGY010000053 DAOMGY010000000
VERSION     DAOMGY010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23987_length_3928_cov_2.442809"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLR57_01475"
     CDS             <1..840
                     /locus_tag="PLR57_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQQ40153.1"
                     /translation="LLNATVAETIYNMVDFGSNAGDATIALCAGMFAIVLWATIAWWF
                     GIPTSESHALIAGISGAAVAIQNGFSGINGSEWIKVIYGLLLSSVLGFFLGYGTSKLV
                     KFLFRKMERRKADSFFKKGQIAGSAAMAFMHGAQDGQKFIGVFLLGTFLAGGQTDVTT
                     FTIPIWLMILCSLVMSLGTSIGGLRIIKAVGMDMVKLDKYQGFSADIAAAACLLLSSL
                     TGIPVSTTHTKTTAIMGVGASKRISSVNWGVVKEMVYAWVLTFPCCGLVGFLMVKLFL
                     AIF"
     gene            885..1511
                     /locus_tag="PLR57_01480"
     CDS             885..1511
                     /locus_tag="PLR57_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HQQ40154.1"
                     /translation="MKKQHVNDYFEGFVTGMERACEAAEYLETVLEHYEPEKLADQLE
                     EMHKIEHEADIEKHNLMQKLAKEFITPIEREDIILLLQQIDNVTDFIEDVVRKMFMYN
                     VTSIRSEAIEFAAIIRQCCTESRDALRELPGFQKSNARLKEAVIKVNDFEEAGDRLYC
                     AAIRRLYTEHAEPLERITWTNMFDWLEACCDACEDVMESVEMVILKNS"
     gene            complement(1556..2902)
                     /gene="murF"
                     /locus_tag="PLR57_01485"
     CDS             complement(1556..2902)
                     /gene="murF"
                     /locus_tag="PLR57_01485"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQQ40155.1"
                     /translation="MKPVQFREILSACGGTYYGDAALLDTVPTDIVIDSRVATPGSLY
                     IPIVGERFDGHEFIDNAREKGSVLVLSAKPLESEPYILVSDTLRALHAIASWYRDLFP
                     IPVVGLTGSVGKTSTKEMIASVLSARFNVMKTIGNKNNETGVPLTLFTLDESHEVAVV
                     EMGTNHFGEIGRLAAMAKPQICVFTNIGVAHIENFGSREGILKGKTEMLAHMQAGGTV
                     IVNGDDDMLATIPGAMRFGFSDACDLRAVDVLDQGLDGVSFTACYKGNEARAHVPSPG
                     LHSVSNALAAIAVGLTLHLGLEELAEGVGAFAAPPGRMNIKQTPRFRILDDTYNANTV
                     SMMTAVDVLEKVQGRKVCILGDMLELGRESAEHHEVLGMYAAMHGIDLIICVGPESES
                     MFLGAIDIAPRRARYFETQKNLLSILPDLVRDGDTILVKASRGMHLEETVQFLMNL"
     gene            complement(2968..>3928)
                     /locus_tag="PLR57_01490"
     CDS             complement(2968..>3928)
                     /locus_tag="PLR57_01490"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HQQ40156.1"
                     /translation="SKGHNAVLVDLFFGVDALPANPADAFAVSGMLPQFEVPEKAPDL
                     QAVRASRKAGFSDQIGTGVLELCRAADITFLALHGGIGENGNLQAFFDLSGVKYTGSP
                     SIGCALAMDKYVSKSLFRSDGIETAAWAMARRGQPFDTSFPVPCVVKPNCGGSSIGVS
                     IVRDAASLASAIEAGFEFEDELIVEEYVPGMELSAGVLGSGEDAVALPLIEIVPKHGF
                     YDYQHKYQKGWTDEIVPARISPELTAKVQQLAVEAFRALKLSVYARIDFLLTPDGRAV
                     CLEANTLPGMTPTSLLPQEAEHYGLDYASLCEAIVSYSLKKYE"
BASE COUNT          856 a         1100 c         1116 g          856 t
ORIGIN      
        1 ctgctcaacg ccaccgtcgc ggagacgatt tacaacatgg tcgactttgg cagcaatgcc
       61 ggagacgcga ccattgcact ctgcgctggc atgttcgcca ttgtgctgtg ggcaaccatc
      121 gcctggtggt tcggcattcc aaccagcgaa agccacgcgc tgattgcggg catctcgggc
      181 gccgcggttg cgattcaaaa cggcttttcc ggcatcaacg gttcggaatg gatcaaggtt
      241 atttacggat tattgctctc ctccgtgctg gggttctttt taggatacgg aacgtccaaa
      301 ctggtcaagt tcctcttccg caaaatggaa cgccgcaagg cggacagctt ttttaaaaaa
      361 ggccagattg caggaagcgc ggcaatggcg tttatgcacg gcgcgcagga cggtcagaag
      421 ttcattggcg ttttcctgct tggaacgttt ttagcgggcg gtcagaccga cgttacaacg
      481 tttaccatcc ctatctggct gatgattctg tgctcgctgg tcatgtcgct cggcacctct
      541 atcggcggtc tgcggattat caaggcggtc ggcatggata tggtcaagct cgataaatat
      601 cagggcttct cggcggatat cgcggcggcg gcctgcctgc tgctctcgtc gctcaccggc
      661 attccggtca gcacgacgca taccaagacc accgccatca tgggcgttgg cgcgagcaag
      721 cgcatcagtt ccgtcaactg gggagtagtg aaagaaatgg tctacgcatg ggtgctgacg
      781 ttcccgtgct gcgggcttgt tggattttta atggtcaagc tgttccttgc gattttctaa
      841 aacgcagcgg ataccaacat acgtaatatt ggaggagaga agaaatgaaa aagcaacacg
      901 tcaacgatta cttcgaaggg tttgtcaccg gcatggagcg cgcctgcgaa gcggcggagt
      961 acctggaaac cgttctggaa cactacgagc cggaaaagct ggccgaccag ctggaagaaa
     1021 tgcataaaat cgagcacgaa gccgatattg aaaagcacaa tctcatgcag aagctcgcca
     1081 aggagttcat caccccgatt gagcgcgaag acatcatcct cctgctgcag cagatcgaca
     1141 acgttacgga ctttatcgag gacgtcgttc gcaagatgtt catgtataac gttacttcca
     1201 tccgctccga agcgattgag tttgccgcca tcattcgcca gtgctgcacg gaatcgcggg
     1261 acgcgctgcg cgaactgccg ggattccaaa agtcgaacgc acgtttgaag gaagccgtca
     1321 tcaaggtcaa cgattttgaa gaagcagggg acaggctgta ctgcgccgct atccgcaggc
     1381 tctataccga gcatgccgag ccgctggagc gcatcacctg gacgaacatg ttcgactggc
     1441 tcgaagcctg ctgcgacgcc tgcgaggacg tcatggaaag tgtagagatg gttatattga
     1501 aaaacagcta aggataaaat caaaagcccg tcggccttcg gccgacgggc ttttattata
     1561 agttcatcaa gaattgaacc gtttcttcca gatgcatgcc gcgcgaggct ttgaccagta
     1621 tcgtgtcccc gtcccgaacc agatcgggca gaattgaaag caggttcttc tgcgtttcaa
     1681 aataccgtgc ccggcgtgga gcgatatcga tggcgcccag aaacatggac tccgactccg
     1741 ggccgacgca gatgatcaaa tcgattccgt gcatcgccgc gtacatgccc agcacttcgt
     1801 ggtgttctgc ggactctcga ccaagctcaa gcatgtcgcc gaggatgcag actttgcgtc
     1861 cctgcacttt ttccagcacg tcgacggccg tcatcatgga aacggtgttt gcgttgtagg
     1921 tgtcgtccag tatcctaaag cgcggcgtct gtttgatgtt cattctgccc ggcggagcgg
     1981 caaatgcgcc aacgccttcc gcgagttctt ccagtcccaa atgaagcgta agcccgaccg
     2041 cgatggcggc aagggcattg ctcaccgaat gcaggccggg ggagggcacg tgtgcgcggg
     2101 cttcgtttcc tttatagcag gcggtgaacg aaacgccgtc gagtccctga tccagaacgt
     2161 ctacggcgcg gaggtcgcag gcatccgaaa acccgaaacg cattgcgccg ggtatggtgg
     2221 cgagcatatc gtcgtcgccg ttgacaatga cggttccgcc agcctgcata tgcgcgagca
     2281 tttccgtttt gcctttcagt atgccttcgc gcgaaccgaa gttttcaata tgcgcaaccc
     2341 cgatattcgt aaaaacgcag atttgcggct ttgccatagc cgcgagccgc ccgatctcgc
     2401 caaagtgatt ggtgcccatc tcgacgacag cgacttcgtg gctttcgtcc agcgtaaaga
     2461 gcgtcagcgg gacgccggtt tcgttgttct tgttgccgat ggtcttcatg acgttgaagc
     2521 gcgcggacag cacggaggcg atcatctctt ttgtggacgt tttgccgacg ctccccgtaa
     2581 gtcccaccac ggggatggga aacaggtcgc ggtaccagga cgcgatggcg tgcagcgcgc
     2641 gcagggtatc gctcacgaga atatacggtt cactttccag cggctttgcg cttaaaacga
     2701 gaacggaacc cttttctcgc gcgttgtcga taaattcatg tccgtcgaac cgctccccga
     2761 cgatggggat atagagcgaa ccgggcgttg cgacccggct gtcgatcacg atgtccgttg
     2821 gaacggtatc caaaagcgcg gcgtccccgt aataggttcc accgcaggca gacaaaatct
     2881 cgcgaaactg aacaggtttc atcttatctt accgccgtaa cgttgattac ttgcttctta
     2941 gcgcaggttg gacagcgttt gatccggcta ttcgtatttc ttgagagaat acgatacgat
     3001 cgcttcgcaa agcgacgcgt agtccagccc gtaatgctcc gcttcctgcg gaaggaggct
     3061 tgttggcgtc atgccgggca gggtgttggc ttccagacag accgcgcgtc cgtcgggcgt
     3121 aagcagaaag tcgatgcgcg cgtacacgct gagcttgagc gcgcgaaacg cttccaccgc
     3181 gagctgctgc accttcgccg tgagttccgg cgagatgcgg gcgggcacga tttcgtccgt
     3241 ccagcccttt tgatacttat gctgataatc gtaaaacccg tgttttggca caatctcgat
     3301 cagcggcaga gccacggcgt cttcgccgct acccagcacg cccgccgaga gctccatacc
     3361 gggaacatac tcttccacga tcagttcatc ttcaaactca aaacccgcct cgattgcgga
     3421 agcaagagaa gccgcgtcgc gcacgatgct gactccgatg ctggagccgc cgcagttggg
     3481 cttgaccacg cagggaacgg gaaagctcgt gtcaaagggc tgcccgcgac gcgccatggc
     3541 ccaagctgcg gtttcgattc cgtcgctgcg gaacagcgac ttggaaacat atttatccat
     3601 tgccagcgcg cagcctatgg aagggctgcc cgtatatttt acgccactga ggtcaaaaaa
     3661 cgcctgcagg tttccgtttt cgccgattcc gccgtgcaac gcaagaaacg tgatgtccgc
     3721 cgcgcggcac agttcaagta cgccggttcc gatctgatcg gaaaatccgg ctttgcggga
     3781 tgcgcggacc gcctgaagat ccggcgcttt ttccggaacc tcgaactgcg gcagcatacc
     3841 gctgacggca aacgcgtccg ccggatttgc gggaagcgca tccactccga aaaacaggtc
     3901 taccaatacg gcgttgtgtc cttttgag
//
LOCUS       DAOMGY010000054         3923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_24037_length_3923_cov_3.266546, whole genome shotgun sequence.
ACCESSION   DAOMGY010000054 DAOMGY010000000
VERSION     DAOMGY010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24037_length_3923_cov_3.266546"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(350..859)
                     /locus_tag="PLR57_01495"
     CDS             complement(350..859)
                     /locus_tag="PLR57_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ40157.1"
                     /translation="MQAVTIRQADREDTPAIARIINASWRAAYAGIVPQEVLNALSDE
                     KKQSQLAAGLERYSEMRYYIVEADGVPVGAACLHPAREADLPEAGEFSFFYLLPAFWR
                     SGYGTLLLAHVEREAKTRKYPILCCWTLEVNARAIAFYEKNGFRRDGARQVVTIGKPL
                     DVIRFVKQL"
     gene            complement(1037..3445)
                     /gene="leuS"
                     /locus_tag="PLR57_01500"
     CDS             complement(1037..3445)
                     /gene="leuS"
                     /locus_tag="PLR57_01500"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQQ40158.1"
                     /translation="MLERYDPQSIELKWQKNWADARCYEAKTGDPRPKYFSMIEFPYP
                     SGQGLHVGHPRPFTAMDVISRKRRLEGYNVLYPIGYDAFGLPTENYAIKTGIHPSKVT
                     HDNIIRFRNQLQRLGYSFDYSREVNTTDPDYYKWTQWIFLKFFEKGLAYKAEIPINFC
                     TSCKVGLANEEVVDGVCERCGGQVVRMVRSQWMLKITAYAQRLIDDLDTVDYLEKIKA
                     QQINWIGRSEGALVNFPLDGTGDTLTVYTTRPDTLFGATYMVVSPEHPLVEKYADRIT
                     NLSAVRAYQKAAAAKSDFERSELAKDKTGEPLEGISAINPVNGKKIPIWISDYVLMGY
                     GTGAIMAVPGHDERDYAFAKTFGLPIIEVVAGGDIEQEAYTECQHGKMVNSGFLDGME
                     VSEAIPAIIDWLEKEKIGERKVNYKLRDWVFSRQRYWGEPIPLVYCEHCGWVPIPYSE
                     LPLKLPMLDDFTPTDDGSSCLARAKDWLHTTCPKCGGPATREADTMPNWAGSSWYFLR
                     YTDPQNDKEFASKEALDYWTPVDWYNGGMEHTTLHLLYSRFWHKFLYDCGYVPTSEPY
                     MKRTSHGMVLGSNGEKMSKSRGNVVNPDDYIPVYGADSFRTYIMFMGAFDQPIPWNEQ
                     GTRGCRRFLDRVWTLQEMVQGEGDTDADQKRAVHATIKKVGEDYERMKFNTAIASMMS
                     LTNDFYAKGKLTKDELHTLILLLAPVAPHICEEINERLGYAPLENAVWPVYDESALVE
                     DVVEYGVQVNGKVRARVTLSASMDTKAVEEAALACEDLKPFLEGKSVKKVIVVRNIVN
                     IVVA"
     gene            3606..>3923
                     /locus_tag="PLR57_01505"
     CDS             3606..>3923
                     /locus_tag="PLR57_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40159.1"
                     /translation="MEQNGVIMTPEQQHNKSAVTAMVLGIVGLVITWFPIVGLVLSIV
                     GFSVARSNRRFAAQNGLKENSMNEAGYVCGLIGLIAGILTILLTILVVVGVFAFAGTW
                     IYQN"
BASE COUNT          771 a         1080 c         1135 g          937 t
ORIGIN      
        1 ccgcaatcaa gaaaaagaag ctctgggggt tcggcgtgta cacggcgctg ctgggtatcc
       61 tcgtcatcgt cgatcaaacg gttacgcatt tcttcctgta aaatagagtt ttaaaatggg
      121 aacttacttt tctgatcagg ggttccaccc ctgcgacaat ttcagtcagg ggttccaccc
      181 ctgcgacaat ttcagtcagg ggttccaccc ctgaagctat ttcattcagg ggttccaccc
      241 ctgaaaccct gaccaaaggg cgcgcccttt ggaaacccac ggctatgtgc aatagaaaaa
      301 gagttcggga gaaatcccga actcttttat cgttgtttta ttactgcttt tatagttgct
      361 ttacgaatcg aatcacgtca agcggtttgc cgatcgtcac gacctgcctt gcgccgtcgc
      421 ggcgaaaccc gtttttctcg tagaacgcaa tcgcgcgcgc gttcacttcc agcgtccaac
      481 aacagagaat tggatacttg cgcgtttttg cctcgcgctc tacatgcgcg agaagaagcg
      541 tgccgtagcc actccgccag aaagctggca gcaggtaaaa gaagctgaac tcgccggcct
      601 ccggcaagtc cgcttcccgc gcggggtgca ggcacgccgc tccgacggga acgccgtccg
      661 cttccacaat ataatagcgc atttcggagt atcgctccaa ccccgcggca agctggctct
      721 gcttcttttc gtcggaaagc gcgttaagaa cttcctgcgg gacgatgccc gcgtacgccg
      781 cgcgccacga cgcgttgatg atgcgggcga tggcgggcgt atcttcgcgg tctgcctgac
      841 gaatcgtgac agcttgcatg gaaaaggctc ctttccgtat ggtatacgac gtttgttgcc
      901 gttaacggca gtataacgcg ttccggcgcg ccgcgcaaac ggctcttact ggagaaactg
      961 gttgtttccc gtgaaacatt cggtaattaa caaacggcgc ggagcaatcc gcgccgttgt
     1021 gcgttttttc gctttgttat gctacgacga tgttgacgat gttacgaacc acgatgacct
     1081 tcttgacgct cttgccttcg aggaatggct tgagatcctc acaggcgagc gcagcttctt
     1141 ctacggcttt cgtgtccatc gaagcggaga gcgtaacgcg cgcgcgcact ttgccgttga
     1201 cctgcacacc gtactccaca acgtcctcaa cgagagcgct ttcgtcgtaa accggccaga
     1261 ccgcgttctc gagcggcgca tagccgagac gttcattgat ctcctcgcag atatgcgggg
     1321 cgacgggcgc gagcagcaga atcagcgtgt gcagctcgtc tttggtgagt ttgcccttgg
     1381 cgtagaagtc gttggtgagc gacatcatgc tcgcgatggc ggtgttgaac ttcatgcgct
     1441 catagtcctc gccgaccttt ttaatagtgg cgtggacggc acgcttttga tccgcgtcgg
     1501 tatcgccttc gccctgcacc atctcctgaa gcgtccagac ccggtcgagg aagcggcggc
     1561 agccgcgcgt accctgctcg ttccagggaa tcggctggtc gaacgcgccc atgaacatga
     1621 tgtacgtgcg gaaggagtcc gcgccgtaaa cgggaatata atcgtcgggg ttgacaacgt
     1681 tgccgcggga cttgctcatc ttttcgccgt tgctgccaag caccatgccg tggctcgtgc
     1741 gcttcatgta tggctcgctg gtcggaacgt atccgcagtc gtagaggaac ttatgccaga
     1801 agcgcgagta cagcaaatgc agcgtggtgt gctccatgcc gccgttgtac cagtccacgg
     1861 gcgtccagta gtcgagcgct tccttggaag cgaactcctt atcgttctgc gggtcggtat
     1921 aacgcaggaa ataccagctc gaacccgccc agttaggcat tgtgtccgct tctcgcgttg
     1981 cagggccgcc gcacttgggg caggtggtgt ggagccagtc ctttgcgcgg gccaggcagc
     2041 tgctgccgtc gtccgtgggc gtaaagtcgt ccagcatcgg cagtttgagc gggagctcgc
     2101 tgtagggaat cggcacccag ccgcaatgtt cgcaatagac gagcgggatg ggttcacccc
     2161 agtaccgctg gcgggagaag acccagtcgc ggagcttgta gttgacctta cgttcgccga
     2221 tcttttcctt ttcgagccag tcgataatgg cgggaattgc ttcggaaact tccatgccgt
     2281 cgaggaagcc ggagttgacc atttttccgt gctggcattc ggtgtaagct tcctgttcga
     2341 tatcgccgcc cgcgacgact tcgatgatcg gcaggccgaa cgtctttgca aacgcgtagt
     2401 cgcgctcgtc gtgaccggga acggccataa tcgcgcccgt gccgtagccc atgagcacat
     2461 agtccgaaat ccagatcgga atctttttac cgttgaccgg attaatcgcg gagatgccct
     2521 ccagcggttc gcccgtttta tcctttgcca gttcgctgcg ctcaaaatcg ctcttggcgg
     2581 cggcggcctt ctggtaggcg cgcacggcgg agagattcgt aatcctatcc gcgtacttct
     2641 ccacgagcgg atgctcgggg gagacgacca tgtaggtggc tccaaacagc gtatcggggc
     2701 gggtggtgta aaccgtcagc gtgtcgcctg ttccgtcgag cgggaaattg accagcgcgc
     2761 cttcgctgcg gccgatccag ttgatctgct gggctttgat tttttcgagg tagtccaccg
     2821 tgtccagatc gtcgatgagg cgctgggcgt acgcggtgat tttgagcatc cactgggagc
     2881 gcaccatgcg gacgacctgt ccgccgcagc gctcgcagac gccgtccacg acctcctcgt
     2941 tggcaaggcc gaccttgcag gaggtgcaga agttgatcgg gatttccgcc ttgtaggcga
     3001 gtcccttttc aaagaacttt aaaaagatcc actgtgtcca cttatagtaa tcagggtctg
     3061 tcgtgttgac ttcacgcgaa taatcaaagg aatagccaag ccgctgcagc tgattgcgga
     3121 agcggatgat gttgtcgtgt gtgactttgg aggggtgaat gcctgtttta atggcgtagt
     3181 tctccgttgg caggccgaac gcgtcgtacc cgatggggta gagcacattg tagccttcca
     3241 ggcggcgctt gcgcgaaata acgtccatgg cggtgaacgg ccgcgggtga ccgacgtgca
     3301 gcccctgccc ggaaggatag gggaattcga tcatggaaaa gtatttcggc cttggatcgc
     3361 cggtttttgc ctcatagcag cgggcgtccg cccagttttt ctgccatttc aattcaatgg
     3421 actgcgggtc gtaacgttcg agcatccgtg tattcctccc aaagtatcta aatacgtaga
     3481 atatcctctt tggcgttttt tgtcaatcga gcgcttgctt tacggattgc gcatcgcgtg
     3541 ctacaatcta tttgtggaat tatacggttc gtaaaaaatc caaaatatgc aaggagggtt
     3601 ttgctatgga acagaacgga gtcattatga cgcctgaaca gcagcataat aaaagcgccg
     3661 tcaccgcaat ggtgctcggc atcgtagggc tggtgattac ctggttcccg atcgtggggc
     3721 tggtgctctc cattgtcggt ttttccgttg cgcgctccaa ccgccgcttt gccgctcaga
     3781 acgggctgaa agaaaacagc atgaacgaag cgggctatgt ctgcggtctg atcggtttga
     3841 tcgcgggcat tctgacgatt cttttgacga ttctggtcgt cgtcggcgtc ttcgcttttg
     3901 cgggcacctg gatctaccag aat
//
LOCUS       DAOMGY010000055         3913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_24126_length_3913_cov_3.145153, whole genome shotgun sequence.
ACCESSION   DAOMGY010000055 DAOMGY010000000
VERSION     DAOMGY010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24126_length_3913_cov_3.145153"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLR57_01510"
     CDS             <1..642
                     /locus_tag="PLR57_01510"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQQ40160.1"
                     /translation="LLLKKARPDRLLRARKAYWIVLLAAAVLIAAALILLNTALSGVP
                     LGGAENYLRFSESWGTDRGKIWAFCLRVYGSFSPIQKLFGGGPGALYHADALAHVFTD
                     AALDTAHNEYLQYLLVSGGAGLLSFLGFLALTLITGAKKSLSDPRSRGLIVAVAAYAV
                     QALVNISQPASTPLFFVFCGLILSISAQPDDVLWRKSRFARWISRRKTRAKNA"
     gene            complement(760..1566)
                     /locus_tag="PLR57_01515"
     CDS             complement(760..1566)
                     /locus_tag="PLR57_01515"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQQ40161.1"
                     /translation="MRRRSVRITPLGYIVLSIIILVMLVSIYFIVWSMRGSKNEDLTA
                     STSPSGYTPTPSLAPVGADTPNPTVAPAADTPTPAETATMTPKQDDTPEPATKTPSPE
                     QVQSAVDGTLNSSGVYMRAGPGQSYSRVGTYSSGTQLKIYEVVYGTGGELDYYFVKVV
                     KENLYGYIAAKFVDKAGLLPGESATPTPKAIAGTIPGTVSASVVALRTMPTTEGNTPI
                     GEAKKGDSVLIYFKTGDFYYIQVVATGVQCYAAAKFITPSTTVPTGTPVP"
     gene            complement(1793..3556)
                     /locus_tag="PLR57_01520"
     CDS             complement(1793..3556)
                     /locus_tag="PLR57_01520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQQ40162.1"
                     /translation="MDLITDSRALMMVKQLLDNTSTVRPLIDLQSQKPKFTPSVINKP
                     FPRATPESQSVPSARVAAFLNDMRDDPTLDMHGVLVLRNGKLLTEATFGAYDQHIWHI
                     THSECKSITGLAIGILIGEGRLKLEDKLVDLFAGKISRINQLTHRNITVKHLLTMTSG
                     ITFNEAGSVTETDWVKCFLESVVLKEPGTSFNYNSMNTYMLGCIIKQVTGVGLTDYLT
                     PRLFAPLGITNFFWEKCPMGNEKAGWGLYILPEDIAKIGQMVLDGGRFEGKQIVPEEW
                     IKEATRSQAATPKTLGDYDYGYQIWVGRNERSFLFNGMFGQNVLGFFDTGVLLVSNAG
                     NNELFQQSSYYALATKHFSREEAFSELKPKLGAGLGALQNAKKRLCADLYTPPIELFG
                     DLFAQRGKLDIGAIFKRLDGKAYFAAGDKRASPVGVYPLYAQTLQNNFTKGLHALSFV
                     CRNGTLQIVIDETDERHVLEIGFDAPAYNDFATHGEVHRVGVSGRFGADESGRPVLLL
                     RVSFLEAANARLIKIRFFDGSIVTEWSESPGKGYLTDALTSVKGQSSLLSGILERSDE
                     ELLLFRITRILEPQVVLNERG"
BASE COUNT          772 a         1120 c         1112 g          909 t
ORIGIN      
        1 ctcctgctga aaaaagcgcg gcccgaccgc ctgctgcgcg caagaaaggc ctattggatc
       61 gttcttcttg ctgcggcggt tctcattgcc gccgcgctga ttctgctcaa cacggcgctt
      121 tccggcgttc cgcttggcgg cgcagaaaac tatctgcgtt tttccgaaag ttggggaacc
      181 gaccggggca aaatctgggc gttctgcctg cgcgtatacg gctccttctc ccctattcag
      241 aagctctttg gcggcggccc cggcgcgctg tatcacgcgg acgcgctcgc ccacgtcttt
      301 accgacgcgg cgctggatac cgcgcacaac gaatatctgc agtatctgct cgtttccggc
      361 ggtgcggggc tattgagctt tctcgggttt cttgcgctca cgctgataac cggcgcaaag
      421 aaaagcctgt ccgatccccg ctcgcgcgga ctgatcgttg ccgttgcggc ctatgcggta
      481 caggccctgg tcaacatctc tcagcccgcg tccacgccgt tgtttttcgt gttttgcggt
      541 ttgattctct ccatttccgc tcagccggac gatgttctct ggcgcaaatc gcgcttcgcg
      601 cggtggatta gccggcgaaa aacacgcgca aagaacgcgt aatactccgt ttgttatcga
      661 tatgaagaat aacgattgac ccgtgttcag cgggcaaaaa acagccccgc ggcattgtct
      721 gccgcggggc tgttgtatca aagcgcttgt gtttggagct tacggaaccg gcgtaccggt
      781 cggaaccgtg gtgctggggg tgatgaactt tgccgctgcg tagcactgca cgcccgttgc
      841 caccacctga atgtagtaga agtccccggt cttaaagtag atcagcacgg agtctccctt
      901 tttcgcctca ccaatcggtg tgttgccttc cgtagtcggc atggtgcgca gcgcaacgac
      961 gctggccgaa acggtgcccg gaatcgtgcc cgctatagct ttgggcgtcg gggtagcgga
     1021 ttcgccgggc agcaggcccg ctttatccac aaacttggcg gcgatgtaac cgtagaggtt
     1081 ttcctttacg accttcacaa agtaatagtc gagttccccg ccggttccgt agacgacttc
     1141 ataaatcttg agctgggttc cgctcgagta cgtgccgaca cgcgaatagg attgcccggg
     1201 gccggcgcgc atatagacgc cgctcgaatt gagcgtaccg tccacggcgg actgcacctg
     1261 ctcgggactg ggcgttttag ttgcgggttc cggcgtatcg tcctgcttgg gcgtcatggt
     1321 cgccgtctcc gcaggcgtgg gcgtatccgc agccggagca acggtcggat ttggcgtatc
     1381 cgcgccaacc ggcgccagcg aaggcgttgg cgtgtatccg gatggcgacg tactcgccgt
     1441 aaggtcttca ttcttggaac cgcgcatcga ccagacgatg aagtaaatgc tgaccagcat
     1501 caccagaatg atgatgctca acacgatata ccccagcggt gtaatgcgta cggatcttct
     1561 cctcattgtc ttttccccca aaaacagcaa gcgtgcgtta atatgatgta atttgactgc
     1621 gaaagcgaat gcctatgcac agtatattgg ttttctctgg catttcaagt ggttttcctg
     1681 aaaaattcat aaagtgttgc agcctgttct aaaagaattc acttttcttt ctcaggcgcc
     1741 cgcagggtaa aatccccgtc cgcgccgcaa aaaaacggcg tttttcgttt gtttatccgc
     1801 gctcgttcag gacgacctgc ggctcaagga tgcgggtgat gcggaagagc agcagctctt
     1861 cgtccgagcg ttcgaggatg cccgaaagca gcgaactctg tcccttgacg gaggtgagcg
     1921 cgtcggtgag ataccctttt ccgggcgatt ccgaccattc ggtcacaata ctgccatcga
     1981 agaaacgaat cttgatgagc cgggcgttgg cggcttccag gaacgaaacg cggagcagca
     2041 gaaccggtct tccgctttcg tccgcgccga accgcccgct gacgccgaca cggtggactt
     2101 ccccgtgcgt ggcaaaatcg ttgtacgcgg gcgcgtcgaa gccgatctcc agcacgtgcc
     2161 gctcgtccgt ttcgtcaata acgatctgca gcgttccgtt ccggcagaca aatgagagcg
     2221 cgtgcagacc cttggtaaag ttgttctgca gcgtctgcgc atagagcggg taaacgccga
     2281 ccggggaagc gcgcttgtct cccgcggcaa aataggcctt accgtcgagg cgcttgaaga
     2341 ttgcaccaat gtcgagcttt ccccgctggg caaacaagtc gccaaacagc tcgatcggcg
     2401 gcgtgtagag atcggcgcag agtcgttttt tggcgttctg cagcgcgcca agtcccgcgc
     2461 cgagcttcgg cttcaattcc gaaaacgctt cctcacggga aaaatgtttg gttgcaagcg
     2521 catagtagct gctctgctgg aagagttcgt tgttgcccgc gttggaaacg agcagcacgc
     2581 cggtatcgaa aaagcccagc acgttctgac cgaacatgcc gttaaagagg aacgagcgct
     2641 cgtttctgcc gacccagatc tgatagccgt aatcgtagtc cccgagcgtc ttgggcgttg
     2701 ccgcctgcga gcgcgtcgct tccttaatcc attcctccgg cacgatctgt ttgccttcga
     2761 accttccgcc gtcgagcacc atctgtccga tcttggcgat gtcctccggc aggatataaa
     2821 gcccccagcc ggccttttcg ttgcccatgg ggcacttctc ccagaagaag ttcgtaattc
     2881 caagcggcgc aaacagccgg ggcgtgaggt agtccgtcag cccaacgcct gtgacctgct
     2941 tgatgatgca gcccagcatg taggtgttca tgctgttgta gttaaagctc gtgccgggtt
     3001 ccttgagcac caccgattcg aggaagcact tcacccagtc ggtctccgtt acggaacccg
     3061 cttcgttaaa ggtgatcccg ctcgtcatgg tcaacaggtg cttgaccgtg atgttgcggt
     3121 gcgtcagctg gttgatgcgg ctgatcttgc ccgcgaagag atcgacgagc ttgtcctcca
     3181 gcttcagcct gccctcgccg atgaggatgc cgatagccag acctgtaatg gacttgcact
     3241 ccgaatgcgt aatatgccaa atgtgctggt catacgcgcc aaacgttgcc tccgtgagga
     3301 gtttgccgtt tctgagcacc agtacgccgt gcatatccag cgtggggtcg tcccgcatgt
     3361 cgttgaggaa cgccgcgacc cgcgcggaag gaacgctctg ggattccggc gttgcgcgcg
     3421 ggaatggttt attgatcacg gagggggtga atttcggttt ctgcgactgc agatcgatca
     3481 gcgggcgcac cgtggaggta ttgtccagga gttgcttgac catcatcagc gcgcgggagt
     3541 cggtaatcag gtccatacgc gatcctccga atcatttcat tctttcattt cgtgtcttcc
     3601 tgcgcgaatg gttctctctt gaccgccaca ggaagaacgc ttaaattaaa ttataccaaa
     3661 aaacgcgtcg tgaaaacaga gtgtgcatac cttgtatcca gtattcagca cagaattgta
     3721 cagtcagttc gaaagtttgt tccgccgacg cgtgctggtt gtggtataat atttcatacc
     3781 agagaggggg cgcctccatc gatggaacgc gacatctata ttcagaacaa ctggtccgcg
     3841 caggacgttg agttgctcaa agcggaattc gcgctgtata accaccgcgc ggtcacccgc
     3901 tttgcctgcg act
//
LOCUS       DAOMGY010000056         3901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_24251_length_3901_cov_3.573843, whole genome shotgun sequence.
ACCESSION   DAOMGY010000056 DAOMGY010000000
VERSION     DAOMGY010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24251_length_3901_cov_3.573843"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /locus_tag="PLR57_01525"
     CDS             complement(<1..1450)
                     /locus_tag="PLR57_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40163.1"
                     /translation="MLKIGKTRILAFALALMMALVLLPAATLAEGDAGHIVLFSDVTD
                     DTEKSYLQTATAAPGETMTVYVPILNKDLASAADLACALAVSSDVKVYPFDQPTDATI
                     KALSAGAYQMWNATDSKLVSWDGKALAQGERAYFKLSYKISASTPTGGYTLPFTVSYS
                     DGSNSLTDTVNVYVYVNEKTSSSSSGGSSYKSKPKVILESYSFVEDTIYAGDTITLRL
                     VVSNTSEREAITNLQLDYSNDAGAILPAPGGSSSIFIGTIDKSDAYAFSIRLQIAPDA
                     EAKSQLLTVKLSYEGTRNRQEFEETASVSVPVQQKARVRINDPVVYDDPWVGGTVSVG
                     LTLYNLGKSPLYNCLVDLEDTPNMTLEEAYFGGNVASGGTMRADLSITPLVGGEVTGN
                     IRVTYEDVYGNQTEQLLPITMLVNEDTGNGGANIYVSGSNAVIPAEIPVDAPASSGLA
                     WYWIALIVCAVIGAAAIIIVRVKKKRERSLEDD"
     gene            complement(1443..2198)
                     /locus_tag="PLR57_01530"
     CDS             complement(1443..2198)
                     /locus_tag="PLR57_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40164.1"
                     /translation="MQENALIDVRSVKKDYHVGTVTVKALRGVDLKIEEGEICSIIGR
                     SGSGKSTLLNVLAGLEKVTAGEIVIAGKHLERMTQGELILFRQKHIGFIFQSFNLMPY
                     YTALENVALPLSFRGVPRELRMKRAARMLEIVGLETHMKHKPSELSGGQQQRVGIARA
                     LVTNPQIVFADEPTGNLDSNTSDEVMRVIIDILREQKKTLIMVTHDPHIADFGDKIIR
                     LLDGHITDIEINPKREEYQNEPEIRKTEVKKDA"
     gene            2538..3827
                     /locus_tag="PLR57_01535"
     CDS             2538..3827
                     /locus_tag="PLR57_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HQQ40165.1"
                     /translation="MQKTYGFDTLQLHAGWRGDPATGAHTVPLYQTSAYLFESAEDAA
                     AQFTGEVPGSIYTRIANPTVAILEERVRALENGQATVCFASGMAALLALVLALCEPGD
                     EIVALSSLYGGSYALLFDQLEHRYGVIAKRVDPEDLSGLAAAITDKTRLVYFESVANP
                     LANIPDIEAIVTAAHDAGVPVACDNTFGTPYLFDAAANGVDFTLHSTTKYFCGNGTSI
                     GGCVTDLGTFSLAGSPRFPQFNLPDAAHHNRVYADMGQDAFAARMRDYYLHDAGFCMS
                     PFNAYLTLLGMQTLSLRMRKHVENADAVAKFLDKNPAVEQVNYARLPESPYFERAKRY
                     LPKGVGGIFTFRIKGGLAAGRRFINALKLFSLVANVGDARSLVVHPASTTHSPLSPED
                     LEKCGISPDLVRLSIGIEDKEDLLADLEQALDASQIV"
BASE COUNT          764 a         1115 c         1131 g          891 t
ORIGIN      
        1 catcatcctc aagcgagcgt tcgcgttttt tcttgactct cacgatgata atcgcagctg
       61 ccccgatgac ggcgcagacg atcagcgcaa tccagtacca ggccaggccg ctgctggcgg
      121 gcgcgtccac gggtatttcc gcagggatga cggcgtttga gccggaaaca tagatgtttg
      181 cgccgccgtt gccggtgtcc tcgttgacca gcatggtgat cggcaggagc tgctcggtct
      241 ggttgccata gacgtcctca taggtcacgc ggatgttgcc ggtgacctca ccgccgacca
      301 gcggggtgat ggagagatcc gcgcgcatgg tgccgccgct ggcgacgttg ccgccaaagt
      361 aggcttcctc cagcgtcatg ttgggggtgt cctccagatc gaccaggcag ttgtaaagcg
      421 gcgatttgcc gaggttatag agcgttaagc ccacggagac cgtgccgccg acccagggat
      481 cgtcgtaaac cacgggatcg ttgatgcgta cgcgcgcttt ctgctgcacc ggcacgctga
      541 ccgaggcggt ttcttcgaac tcctgacggt tgcgcgttcc ttcataggag agtttgacgg
      601 tgagcagttg agacttcgct tccgcgtccg gcgcaatctg gagcctgatg gagaaggcgt
      661 atgcgtcgct cttatcgatc gtgccgataa agatgctgct gctgccgccc ggcgcgggca
      721 gaatggcgcc ggcgtcattc gaatagtcga gctgcaggtt cgtaatggct tcccgctcgg
      781 aggtgttgga aacgaccagc cggagggtta tggtgtcgcc tgcgtaaatg gtgtcttcca
      841 caaagctgta cgattccaga atgactttcg gtttgctctt atagctgctg ccgccgctcg
      901 aagaggaaga ggttttttcg ttgacgtaaa cgtaaacgtt gaccgtatcc gtcaggctgt
      961 tggaaccgtc cgaatagctg accgtaaagg gcagcgtgta tccgccggtc ggggtggacg
     1021 cggaaatctt ataagaaagc ttaaagtaag cgcgttcacc ctgcgccagc gccttgccat
     1081 cccaggaaac aagcttgctg tccgttgcgt tccacatttg gtatgcgccc gcggacagcg
     1141 cttttatcgt tgcatcggtg ggctgatcga acggatagac ctttacgtcg cttgaaaccg
     1201 caagagcgca agcaagatcc gcagcgctcg caaggtcttt gttcaggata ggcacgtaga
     1261 cggtcatcgt ttcgcccggg gcggcggtcg ccgtctggag gtacgatttt tccgtgtcgt
     1321 ccgttacgtc ggagaaaagc acaatgtgcc ccgcgtcgcc ttccgccagc gtggcggcgg
     1381 gcaggagcac aagcgccatc atcagcgcca gcgcgaacgc cagaatacgg gtctttccaa
     1441 ttttaagcat ctttttttac ctcggttttc cgaatttccg gttcgttttg gtattcttcc
     1501 cttttggggt tgatttcaat atctgtaata tgtccgtcga gcaggcggat gatcttgtct
     1561 ccaaagtccg cgatgtgcgg atcgtgcgtc accatgatga gcgttttttt ctgctcgcgc
     1621 agaatatcga tgatgacccg catgacctcg tcgctcgtgt tggagtcgag gttgccggtc
     1681 ggctcgtcgg caaatacgat ctgcgggttg gtcaccagcg cgcgcgcaat gcctacgcgc
     1741 tgctgctggc cgccggaaag ttcgctgggc ttgtgcttca tatgcgtttc cagaccaacg
     1801 atttccagca ttctagccgc gcgcttcatg cgaagctccc gcggtacgcc gcgaaacgac
     1861 agcggcagag ccacgttttc cagcgccgta tagtacggca tgaggttgaa cgactgaaag
     1921 atgaacccga tatgtttctg ccggaacaga atgagctcgc cctgcgtcat gcgctcgagg
     1981 tgcttgcccg caatgacgat ttcgcccgcc gtcacttttt ccagtcccgc gaggacgttg
     2041 agcagcgtgg atttacccga accgctccgc ccgatgatcg agcagatctc gccctcttca
     2101 atcttcaaat cgacgccgcg cagcgctttt accgtcacgg tgccgacgtg gtaatccttt
     2161 ttgacgctgc gaacgtcgat cagcgcgttt tcctgcatga gccctccata acagccatga
     2221 agcacaagtg tattacttgt actaatacgc ctgtatatta ctcttgtttc aaagttctgt
     2281 caatcacgat tccggcgtaa atgaaaacat cgttaaacaa atatgttact tcttgtcctt
     2341 atttgcaaag gcttgaatgc cgcatgcgtt ccgccggggc gggcaaacgg cggaaaacag
     2401 gcggttcttc cgataaatcg cctgatggaa ctccgttttt tagttcaaat atcgttgcat
     2461 gcgtggcata tatctgatag gatagaggaa atctttgcgc aggagcgcga aacgagcgaa
     2521 acaagcgagg cgtcaccatg caaaaaacgt acgggttcga tactcttcag ctgcacgcag
     2581 gctggcgcgg ggatcccgcg accggcgcgc atactgtgcc gctgtatcaa acgagcgcgt
     2641 acttatttga gagcgcggag gacgcagccg cccagtttac cggcgaagtg ccgggcagca
     2701 tctatacgcg tatcgccaac cccaccgtcg ccattttgga agagcgcgtc cgcgcgctgg
     2761 aaaacggaca ggcgactgtt tgctttgcct ccggcatggc ggcgctgctg gcgctggtgc
     2821 ttgcgctctg cgaaccgggg gacgaaatcg tcgccctctc ctcgctctac ggcgggagtt
     2881 acgcgctgct gttcgaccag ttggaacacc gctacggcgt gatcgccaaa cgcgtcgatc
     2941 cggaggacct ttcggggctg gcggcggcca ttaccgacaa aacgcgcctt gtttactttg
     3001 aatccgtcgc aaatcccctt gccaacattc cggatatcga ggcgattgta accgccgcgc
     3061 acgacgcggg agttcccgtt gcctgcgaca atacgttcgg cacgccgtat cttttcgacg
     3121 cggcggcaaa cggcgttgac tttaccctgc actccacaac gaaatatttc tgcggaaacg
     3181 gaacgagcat cggcggctgt gtgactgacc tcggcacgtt ttcgcttgcc ggttcgccgc
     3241 gttttccgca gttcaacctg ccggatgcgg cgcaccataa ccgcgtttat gccgatatgg
     3301 gccaggacgc attcgccgcg cgcatgcggg actattacct gcatgacgcg gggttctgca
     3361 tgtccccgtt caacgcgtac ctgacgttgc ttggcatgca gacgctttcg cttcgaatgc
     3421 gtaagcacgt cgaaaatgcg gacgcggttg cgaagttcct tgataaaaac cccgcggtcg
     3481 agcaggtgaa ctatgcaaga ctgccggaga gtccgtattt cgaacgtgca aagcgctact
     3541 tgcccaaggg ggtcggcggc attttcactt tccgcatcaa agggggcctt gcggccgggc
     3601 ggcggttcat caacgcgctc aagctctttt cgctggttgc caacgttgga gacgcgcgct
     3661 cgcttgtcgt ccatcccgcg agcaccacgc attcgcccct ttcaccggaa gacctcgaaa
     3721 aatgcggcat ttcgcccgac ctcgtccggc tctcgattgg cattgaggat aaggaagacc
     3781 tcctcgccga tctggagcag gcgcttgacg cgtcccagat cgtttgatcg aaaacagatc
     3841 gttttcaatg ggagcgccgg cgcaaagcgg gcgctctttt gcgcccgtaa gcagtactac
     3901 a
//
LOCUS       DAOMGY010000057         3889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_24357_length_3889_cov_3.080073, whole genome shotgun sequence.
ACCESSION   DAOMGY010000057 DAOMGY010000000
VERSION     DAOMGY010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24357_length_3889_cov_3.080073"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLR57_01540"
     CDS             <1..598
                     /locus_tag="PLR57_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40166.1"
                     /translation="EDLQRYLAEYHQMIDQNRKELGVEEEPQPAPVPRQQQAQYTPQQ
                     AYAPAPAYEEPRYSEAQYVEQPRYQEPVAPQAQRAQTARQPRPVSRPVERMAPEEDPA
                     ATRQFARPDRAPVREEVEEAPRRREPARAEEPPKKKGGLFKRLLEAEDDDDEELDDDE
                     SWIEEPAPAKAPKEKKPRRKLIEFIEADDDEEDEDEDY"
     gene            706..1482
                     /locus_tag="PLR57_01545"
     CDS             706..1482
                     /locus_tag="PLR57_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQQ40167.1"
                     /translation="MAKKIGLALGAGSTKGFAHIGVLQQLQEEGIHVDMVSGSSMGAI
                     IGSIYSVGTDLNMLEKIITTMNIREYMDVSFPLTGGLLKGEKLQELIRVLTHNKTFAQ
                     TRIPFCCVAVDANYGKLDVLDHGMLHEAVRASMSIPAIFEPMPFQGKTYIDGGVLERV
                     PCKCLRERGADVVIGVDVGYHGDPEDVTGMNAYQLMNRMISIMTWEMTKLRREEADLM
                     LVPEVLFVKGRFQNDQAGDCVEEGRRVVRENLARIKELIK"
     gene            complement(1599..2801)
                     /locus_tag="PLR57_01550"
     CDS             complement(1599..2801)
                     /locus_tag="PLR57_01550"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQQ40168.1"
                     /translation="MRVVGIIAEYNPLHNGHIYHLERARALSGADYCVVVMSGNFVQR
                     GEAACTDKYTRAAWALKGGADVVLELPSVYAVAPAERFAAGGVRTLAGTGVVTDLAFG
                     CEAPDLRTLYQISDIIVSEPPALQAGIKHHLSLGKAYPRARFDALREYGVPAAMLNAI
                     AQPNNILAVEYLNAIKTYAPSMEPLPITRIGTGYHSEEIAGEFASATAIRKAIIEGRH
                     EILNTMPSFVGGPLLYDDQFIIPQEAVGSFMLYALRRMSVEELAQLPDVGEGFENVLY
                     RAARSARTIEGFLEAVKSKRYTLARCRRITACALLGITSDQVRQTATSREGSYLRVLG
                     FQKTARPLLGEMARRRTMPMFVRNNDLDGCSPMVRRNVETDSLSTDILCFATDRDLKR
                     DLSGPVIL"
     gene            3032..>3889
                     /gene="pta"
                     /locus_tag="PLR57_01555"
     CDS             3032..>3889
                     /gene="pta"
                     /locus_tag="PLR57_01555"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HQQ40169.1"
                     /translation="MSLMESIKQKAKTNKKGILLPEGPEERTVQAVGVIMAEGIADVT
                     LLGNEAEIRESAQKLGVSLNGVEIVDPVLDADRKAYVAAYYELRKEKGVTPEKAESTM
                     KDPLFYAAMMVKKGRGDGMVAGALCTTGETLRPGLQIIKMAKGISVVSSCFIMEVPNK
                     AYGDDGLMLFGDCAVNPCPTAEQLAAIAISSAVTAKSLCGMEPRVAMLSFSTKGSAKH
                     ELVDKVVEATRIAHELAPELNVDGELQADAALVEKVGKLKSPGSTVAGKANILIFPDL
                     QAGNIAVKLV"
BASE COUNT          908 a         1058 c         1173 g          750 t
ORIGIN      
        1 cgaagattta cagcggtatc tggcggaata ccaccagatg atcgaccaga accgcaagga
       61 actgggcgtc gaggaagagc cgcagcccgc gcccgtgccg cgtcagcagc aggcgcagta
      121 tacgccgcag caggcctatg cgcccgcgcc ggcgtacgaa gaaccccgct acagcgaagc
      181 gcagtatgtt gaacagccgc gctatcagga gcccgttgct ccgcaggcgc agagagcgca
      241 gaccgcgcgc cagccgcgcc cggtttcccg ccccgttgag cgcatggcgc cggaagaaga
      301 tcccgccgcg acccgccagt tcgcccgccc cgaccgcgcg ccggttcggg aagaggttga
      361 ggaagcgccg cgccgccgcg aacccgcccg cgcggaagaa ccgcccaaga agaaaggcgg
      421 actctttaag cgcctgctcg aagcggaaga cgacgacgat gaagaactgg acgacgacga
      481 gtcctggatc gaggagcccg ctcccgcgaa agcgcccaag gagaagaagc cccgcagaaa
      541 gctcatcgag ttcatcgaag cggatgacga cgaagaggac gaggacgaag attactaaga
      601 acgcagcgag ccggaagttc aaattccggc tcgttttcac gcggggcggc gattcgcttt
      661 cccgcgtgaa tcgagcgatc tgtttgatgg agaagtaaag ttagtatggc aaaaaagatt
      721 ggcctggcgc ttggcgcggg cagtacgaag ggctttgcgc atatcggcgt tctgcagcag
      781 ctgcaagaag aggggattca tgtggacatg gtctccggca gcagcatggg cgctatcatc
      841 ggctccatct acagcgtagg aaccgatctg aacatgctcg aaaagattat taccaccatg
      901 aacatccgcg agtatatgga tgtttccttt ccgctcacgg gcggcctgct caaaggcgaa
      961 aagctgcagg agctgattcg ggttctgacg cacaacaaga cctttgccca gacccgtatt
     1021 ccgttctgct gcgtcgccgt tgacgcaaac tacggcaagc tcgacgtgct cgatcacggc
     1081 atgctgcacg aagccgtccg cgccagcatg tcgattccgg ctatctttga accgatgccc
     1141 ttccaaggta aaacgtatat cgacggcggc gttcttgagc gcgtgccctg caagtgcctg
     1201 cgtgaacgcg gtgcggacgt ggtcatcggc gtggacgtcg gctaccacgg tgaccccgag
     1261 gacgtgaccg gcatgaacgc gtatcagctc atgaaccgta tgatctcgat tatgacgtgg
     1321 gaaatgacta agctccgccg cgaagaagcg gacctgatgc tcgtgccgga ggtactgttc
     1381 gtcaagggtc gattccagaa cgatcaggcg ggggactgcg tagaagaagg aaggcgcgtc
     1441 gtgcgcgaaa acctcgcgag aattaaagag cttataaagt aactcttctt ttctttataa
     1501 gaaaagaagc aaaagaacct catattttga ggttctttgt tgttcaatag atgaaaattc
     1561 tttttgtttc tttttctttt aagaaaaaga aaaagatctc acagtatcac aggcccgctc
     1621 agatcacgct tgaggtcgcg gtcagttgca aagcagagaa tatccgtgct cagcgaatcg
     1681 gtttcaacgt ttctgcgcac catcggcgaa cagccgtcaa gatcgttgtt gcgcacgaac
     1741 atcggcatcg tgcggcggcg cgccatctcg ccgagcagcg gacgcgcggt tttctggaaa
     1801 ccgagtacgc gcaggtacga tccttcgcgg ctggtggccg tctggcgcac ctgatcgctg
     1861 gtgatgccaa gcagcgcgca ggcagtaatc ctgcgacagc gcgcaagggt gtagcgcttg
     1921 ctcttgaccg cttccagaaa gccttcaatc gtacgggcgg aacgcgcggc gcggtagagg
     1981 acgttttcga acccctcgcc aacgtccggc agctgcgcaa gctcctcaac gctcatgcgt
     2041 cggagggcgt agagcataaa gctgccaacg gcttcctgcg gaataatgaa ctggtcgtcg
     2101 tagagcagtg gcccgcctac gaacgaaggc atggtgttca gaatttcatg ccgcccctcg
     2161 ataatcgcct ttcgaatcgc cgttgcgctg gcaaactcgc cggcaatctc ctcggagtga
     2221 tagcccgtgc cgatgcgcgt aatgggcagc ggttccatgg agggagcata ggttttgatg
     2281 gcgttcaggt actccaccgc gaggatgttg ttcggctggg cgatggcgtt gagcatggcg
     2341 gcgggtacgc cgtattcgcg gagcgcgtcg aaacgcgcgc ggggatacgc cttgccgagc
     2401 gaaaggtgat gcttaatccc agcctgcagg gcgggcggtt cggagacgat gatgtcggaa
     2461 atctgataga gcgtcctgag gtcgggcgct tcacaaccga acgcaagatc ggtgacaacc
     2521 ccggttcccg caagggtgcg cacgccgccc gcggcaaacc gttccgcggg ggcgacggcg
     2581 tatacgctcg gcagttcgag cacaacgtcc gcgccgcctt tgagcgccca ggcagcgcgg
     2641 gtatatttat cggtacaggc ggcttcgccc cgctgaacga agttgccgct catcacaacg
     2701 acgcagtaat ccgccccgga aagcgcacgc gcgcgctcca gatggtagat atgcccgtta
     2761 tgcagcggat tgtactccgc aatgattccg actactcgca tatacctcaa cgattccaca
     2821 gcggcgttta tggaatgaaa ttccataaaa accgatttcg tcagcatttt caacggtttt
     2881 ctgacgattc aagtatacca tttccaattc tgttgtggta tactctagcg gttgggtaca
     2941 caaaatgtaa gaatgatggt agaaaaccac aaatttgtat cattcaattc gacgattttt
     3001 agtgcagcat aaatgggagg cgcaagttgt catgtcttta atggaatcga tcaaacagaa
     3061 agcaaaaacg aataaaaagg ggattctcct gccggagggg ccggaagagc gtacggttca
     3121 ggcggtcggt gtcatcatgg ccgaagggat tgcggacgtt acccttttag gcaacgaagc
     3181 ggaaattcgg gaatcggcgc agaaactggg tgtatccctg aacggagtgg aaattgtcga
     3241 tcccgtgctg gacgcggatc gcaaagcata cgtggccgca tactatgaac tcagaaaaga
     3301 aaagggcgtt acgccggaaa aagcggaatc cacaatgaag gatccgcttt tctatgccgc
     3361 catgatggtc aaaaagggtc ggggcgacgg catggttgcg ggcgcgctgt gcaccacggg
     3421 cgaaacgctt cgcccggggc ttcagatcat aaaaatggca aagggtattt cggtcgtttc
     3481 gagctgcttt attatggaag tgccgaacaa agcctatggc gacgacggac tgatgctctt
     3541 tggcgactgc gcggtcaatc cgtgcccgac ggctgaacag ctggcggcca ttgcgatttc
     3601 ttccgcagtg acggcaaagt cgctctgcgg catggaaccg cgggttgcaa tgctttcgtt
     3661 ctccaccaaa ggcagcgcca agcacgaact ggtcgataag gttgtggaag caacccgcat
     3721 tgctcacgaa ctcgcgccgg aactgaatgt ggacggcgaa ctgcaggcgg acgccgcgct
     3781 tgtggaaaaa gtcggcaagc tcaagtcgcc cggcagcacg gttgcaggca aagcgaacat
     3841 cctgattttc cccgatctgc aggcgggcaa catcgccgtg aagctggtc
//
LOCUS       DAOMGY010000058         3856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_24628_length_3856_cov_2.720600, whole genome shotgun sequence.
ACCESSION   DAOMGY010000058 DAOMGY010000000
VERSION     DAOMGY010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24628_length_3856_cov_2.720600"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PLR57_01560"
     CDS             complement(<1..507)
                     /locus_tag="PLR57_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQQ40170.1"
                     /translation="MSALSGGTKHRPQIKLFFLAVFCIFLWATAIPCVKLSYPLFGVE
                     SSAPFDQIFFAGIRFTGAGLLTLLIAAITKSGARSLQKKHILPTLGLGLVQTTAQYIC
                     FYIGLSNTTGARGAIFNSVSTFFTVLLAALFFRAEKVTPRKLIGCFVGFIGIIVVNLG
                     GGLSSRFTM"
     gene            complement(504..1526)
                     /locus_tag="PLR57_01565"
     CDS             complement(504..1526)
                     /locus_tag="PLR57_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQQ40171.1"
                     /translation="MAKVVGFGDLLVRFSPPGYQRFLQADSYEINYTGAEANVLVNLA
                     LNGVDTEFVTRLPQNLISECAVAQLKKFGVGVKRISFGGDRIGVYYLEKGASQRPSRL
                     VYDRKYTSFAASSPSDYDWDSIFEGVTHFHFTGITPALGPELPGICFAACAQARARGI
                     LTSCDLNYRALLWDLETASKTMRRLMPLVDVLVCGREDSEKLLGVKPESKIVHGDTID
                     REAYAAAAAELTRVYGFQTVAITLRESFSASDNNWSGMLYTGGKAYFSKEYQIHLVDR
                     VGGGDSFTAGLLYAMIHGFDPQHAVEYAVAASCLKQTIEQDFNLSYADEIEKLASGEA
                     SGRIQR"
     gene            complement(1514..2188)
                     /locus_tag="PLR57_01570"
     CDS             complement(1514..2188)
                     /locus_tag="PLR57_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HQQ40172.1"
                     /translation="MSESVVDLIAAHGIIAICRKIYGANLTGLASALQKGGVKLMEVT
                     FDQSDPDGVSRTAEGISQLCAAFPEMQFGAGTVLTREQVDAAKDAGAGFIISPNTDIG
                     LVRYTKSLGLVSIPGAMTPTEIMSAHDAGADYVKLFPASYLGLKYLKDITAPINHVKL
                     LATGGMSADNLGDFLRAGCRGAGISSTLCDKARILAGDWDTLTKTARELVSIFNTVAE
                     EKGWQK"
     gene            complement(2560..3348)
                     /locus_tag="PLR57_01575"
     CDS             complement(2560..3348)
                     /locus_tag="PLR57_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HQQ40173.1"
                     /translation="MEGSSGSHVQVLDRALVLIELLATSEGGMGLAELSAATGLPKST
                     IHRILASYASRHYIEKDSDTSTYFLGHKFVEIASLYLNKIVLKTEAAPIMHEMASIFN
                     AISYLGVTENNEVVYLERAEQFNSLRLYTQIGKREPLYCTALGKVLMSALPEEEFEHI
                     AKQLSYKPYTPNSVTSFEAFKREVQFAREHGYALDIEEHTVGSSCLAVPIYDYTRKVM
                     AAMSISGRGLFDSFSIDYIYQQMKQASMELSKRMGYTQDPASRE"
BASE COUNT          894 a          984 c         1134 g          844 t
ORIGIN      
        1 catcgtaaac cggctcgata ggccgccgcc gaggttgacc acgatgatgc cgataaaacc
       61 gacgaaacag ccgatgagtt tgcgcggagt caccttttcc gcccggaaga acaacgcggc
      121 aagcagcacc gtaaaaaacg tggagaccga gttgaagatt gcgccgcgcg cgccggtggt
      181 gttggaaagc ccgatgtaaa agcagatata ctgcgccgtg gtctgcacaa gccccagccc
      241 gagggtggga agaatgtgtt ttttttgcag agaacgcgcg ccggattttg taatggccgc
      301 aatcagcagc gtgagcaggc ccgcgccggt aaagcggatg cccgcaaaga atatctgatc
      361 gaacggcgcg ctgctttcga cgccgaagag cggatagctc agcttgacgc aggggatggc
      421 cgtagcccag agaaagatgc agaacaccgc gaggaaaaac agcttaattt ggggccggtg
      481 ttttgtgccg ccggacaatg cgctcatcgc tgaatccttc cgcttgcttc cccagaagct
      541 agcttttcga tttcgtcggc gtaggaaagg ttgaagtcct gttcaatggt ttgcttcagg
      601 cagctcgcgg caaccgcata ttccacggcg tgctgagggt cgaatccgtg aatcatggcg
      661 tagagcagac ccgctgtaaa cgaatctccg ccgccgacgc ggtcgaccag atggatttgg
      721 tattccttgg aaaaatacgc cttgccgccg gtatagagca tgccgctcca gttgttgtcc
      781 gaggcggaaa agctttcgcg tagcgtaata gcgacggttt gaaaaccgta tacgcgcgtc
      841 agttccgccg ccgcggctgc ataagcttcc cggtcgatgg tgtcgccgtg tacgatttta
      901 ctctccggct tgacgcccag cagtttttcg gaatcctcgc gcccgcagac gagaacgtcc
      961 accagcggca tgagacggcg cattgttttg gaagccgttt ccaaatccca gagcagcgcg
     1021 cggtagttga gatcgcagct ggtcaggatg ccgcgcgcgc gtgcctgcgc acaggcggca
     1081 aaacagatgc ccggcagttc ggggccgagc gcgggggtga tgccggtaaa gtgaaaatgg
     1141 gtaacgcctt caaagatgga atcccagtcg taatcggacg gggaagacgc ggcaaacgag
     1201 gtgtatttgc ggtcgtagac gaggcgggag gggcgctggg aagcgccttt ttcgaggtag
     1261 tatacgccga tgcggtcccc gccaaaggag attcgcttta cgccgacgcc gaactttttg
     1321 agctgggcaa ccgcacactc gctgatgaga ttctgcggca aacgggtcac aaactccgtg
     1381 tccacgccgt tgagcgcgag attcaccaga acgtttgctt ccgcgccggt atagttgatt
     1441 tcgtagctgt ccgcctgtag aaagcgctgg tagcccggcg ggctgaagcg cacaagcaga
     1501 tcgccaaaac cgactacttt tgccatccct tttcctccgc gaccgtgttg aaaatggaaa
     1561 ccagttcgcg cgcggtcttg gtgagcgtgt cccagtcgcc cgcgaggatg cgcgctttgt
     1621 cgcagagcgt gcttgaaatg cccgctcccc ggcagcccgc gcggaggaag tcaccaaggt
     1681 tatcggcgga catgccgccg gtggcgagca gcttgacatg gttgatggga gccgtgatgt
     1741 ccttgaggta tttcagcccc agatagctcg cgggaaagag cttaacatag tccgcgcccg
     1801 cgtcgtgcgc gctcatgatc tccgttgggg tcatagcgcc cggaatcgaa acgaggccga
     1861 gggattttgt gtagcgaacg aggccgatat ccgtattcgg cgagatgata aagcccgcgc
     1921 ccgcgtcttt ggccgcgtca acctgctcgc gcgtgagcac cgtgcccgcg ccgaactgca
     1981 tttcgggaaa tgccgcgcag agctgagaaa ttccttcggc ggtgcgcgaa acaccatccg
     2041 ggtcggactg gtcgaacgtc acttccatga gctttacgcc gcctttttgc agggcggaag
     2101 ccaaaccggt caggttagcg ccatagattt ttctgcaaat tgcgataatg ccgtgcgcgg
     2161 caatgagatc gacaacggat tcggacatgc acttttacct cgcaattgat gatgatcgaa
     2221 ttagcgaaac gtgattccat aatgtggaac gcaataatta tagcgcgaaa cggtcgggct
     2281 tgtcaatccc ggtttccgct aattggcaaa atcataaaca gaactgcgtg aaaacaaggg
     2341 attccaaagg gagaatccct ttggcaggtg ttcgagggcg gagccctcga attttgaatc
     2401 gcaaaagagc ctcgaatcta aagccgaagg aagctcgagg gcggagccct cgaatttaga
     2461 tcgcagaaga gccctcgaat ttagttcgac gtagtgctct cgaaatattg caaatatcaa
     2521 aaaaggcctc cgcgaaagcg gaggcaaaaa tccaattcct tattccctgg aggcgggatc
     2581 ctgcgtgtat cccatgcgct tggagagctc catggaagcc tgcttcatct gctggtagat
     2641 gtagtcgatg gagaagctgt cgaatagtcc gcggccggaa atgctcatcg cggccatgac
     2701 ttttcgggtg taatcgtaaa tcggcaccgc aagacagctg ctgccgacgg tgtgctcttc
     2761 gatatccagc gcgtagccgt gctcgcgggc aaactgcact tcgcgtttga aggcttcgaa
     2821 cgacgtgact gaatttggcg tgtagggctt gtaggaaagc tgtttggcaa tatgttcaaa
     2881 ctcttcttcc ggcagggcgg acatcagcac tttgccgagc gcggtacagt aaaggggttc
     2941 gcgcttgccg atctgcgtgt agaggcggag gctgttgaac tgttcggcgc gctctaagta
     3001 gacgacttcg ttgttttcgg taacgccgag atacgaaatc gcgttgaaga tgctggccat
     3061 ttcgtgcatg atgggcgcgg cttcggtttt gagcacgatt ttgttgaggt agaggctcgc
     3121 gatctcgacg aacttgtggc cgagaaaata tgtgctcgtg tcggagtcct tttcgatgta
     3181 gtggcgcgag gcatagctcg caaggatgcg gtgaatcgta cttttcggca gaccggtggc
     3241 ggcggaaagt tcggccaagc ccattccgcc ttcggatgtt gccagcagct cgattaagac
     3301 gagcgcgcgg tccagcacct gtacgtgcga cccggaagac ccttccataa acacctccga
     3361 cactcttcta tactccggtt ttttgtttgc caacagatgc gcggcagacg aaaaaacatg
     3421 gagcctgtac gcggcgatag cgtttaccgg ctgataagca ggctttgacc ccgccgcaaa
     3481 tttttacaaa tagcataata acatagtttg tatatgagca caacacccgt tgggaaatac
     3541 aattttcgtt gcgtgtttcg ggggttgcga tcatggcgtg actctggcaa ggcgctgacg
     3601 gaatcctcgc gggcggggtg aaagcctgat aaaaacgctc gtgcgttcgg tggatttgag
     3661 gatgtgaaca cgcgaaatgc atgcgtaact tttgggaaaa acaagcaaaa tcaaacgaaa
     3721 acaggctgaa aagcaggcta aaaaaacgtt tttacgcatt tttttaattt tttttactat
     3781 tttctgaaca aatccactaa gcataataga aaaaaatgac gttggagttc acatttcgtc
     3841 aattcttact ataata
//
LOCUS       DAOMGY010000059         3851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_24669_length_3851_cov_3.018967, whole genome shotgun sequence.
ACCESSION   DAOMGY010000059 DAOMGY010000000
VERSION     DAOMGY010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24669_length_3851_cov_3.018967"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLR57_01580"
     CDS             <1..535
                     /locus_tag="PLR57_01580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ40174.1"
                     /translation="PMNLTIKPLTAELIPAYLDFFDNRAFSATDVNGPCYCTCPTQTS
                     EEIDKMVSEFAGDIKGTLRKHAAKMLAEGKIRGYLAFDGETVVGWCNAAMKNTYVKNR
                     YQFIPDFAWGDDSDAVMSVVCFSIAPAYRGKGVSTALLTRVVDDARATGCSAVEGYAH
                     LRKGEGEFDFKGPEKLY"
     gene            complement(711..1505)
                     /locus_tag="PLR57_01585"
     CDS             complement(711..1505)
                     /locus_tag="PLR57_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HQQ40175.1"
                     /translation="MGQALLMASAGTGFTFLMTTLGAAMVFFFRKSMSGKSQKVFLGF
                     AAGVMIAASVWSLLIPAIEEAAANGKIGWIPAAGGFILGILFLLGLDQIIPHLHPGST
                     DAEGPSTSMKRTSLLVMAVTLHNIPEGMAVGLSFALAAQHGGTPASYAAALALALGIG
                     IQNFPEGAAISLPLRQEGFSVGKSFVYGTLSGIVEPIFGILTVLVSGFIGPYMPWMLS
                     FAAGAMMYVVVEELIPEAHLGEHSNIGTLGVMAGFLIMMILDVALG"
     gene            complement(1820..3136)
                     /gene="xylA"
                     /locus_tag="PLR57_01590"
     CDS             complement(1820..3136)
                     /gene="xylA"
                     /locus_tag="PLR57_01590"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HQQ40176.1"
                     /translation="MAYFSEIGTIRYEGAKSVNPLSFKYYNPDEIVAGKPMKEHLRFA
                     MSWWHTFTYRGVDPFGGVTIFRPWDETENDVIRAKERVHAAFEFMEKMQIGYFCFHDA
                     DLAPRRDTLLETNRILDEVVAEVKQEMTRTGIKCLWGTSNCFGDAKFVHGAATSPNAT
                     VFAYTAAQIKKAIEVTMELGGENYVFWGGREGYETLLNTDMGLELDNFARLLKMAVDY
                     AKKIGFTGQFLIEPKPKEPTKHQYDFDTATVLAFLRKYGLESHFKMNIEANHATLAAH
                     TFQHELCAARINGVFGSVDANTGDPMLGWDTDQFPTNVYDTTLAMYEILLAGGFTSGG
                     LNFDAKVRRGSFQEDDLFLAYIAGMDTFAKGLRAAAKMLEDGTLESFKAKRYESYLEG
                     IGKDIVSGRVGFSELEAYALIHGTNPNASGRQEYLETVLNQYILEF"
     gene            complement(3185..>3851)
                     /locus_tag="PLR57_01595"
     CDS             complement(3185..>3851)
                     /locus_tag="PLR57_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQQ40177.1"
                     /translation="MKISSIIFGAVCCVVYVVLTIFGNINKKKKGYAVENRASLYTRL
                     VIICGVVMLLMYKLSLYKGIPYILLWLIGIVAIYTYITAKTTFGRHFYALGGNEKATK
                     LSGINTNRIYFSAYVNVSFLSALAALVVAARFTSANPAAGTNYELDAIGSCFIGGASA
                     YGGIGKVSGAVIGAVLMGVLNLGMNIIGVDSNFQKVVKGLVLLAAVIFDVVSKRKAIK
                     VAA"
BASE COUNT          849 a         1066 c         1134 g          802 t
ORIGIN      
        1 cccgatgaat ctgacgatca agcccctgac ggccgaactg attccggcat acctcgactt
       61 tttcgacaac cgggcgtttt cggcaaccga cgtcaacggc ccctgttact gcacgtgccc
      121 cacgcagacc tcggaagaaa tcgacaagat ggtgagcgag tttgcggggg atatcaaggg
      181 cacgcttcgc aagcacgcgg caaagatgct cgccgaaggc aaaattcgcg ggtatctggc
      241 gtttgacggc gaaaccgtcg tcggctggtg caacgccgcg atgaagaata cctacgtaaa
      301 aaaccgctac cagttcatcc cggactttgc gtggggcgat gacagcgacg ccgtaatgtc
      361 ggtcgtctgc ttctcgattg cgcccgcata tcgcggcaaa ggcgtttcga ccgcgctttt
      421 gacacgggtc gttgacgacg caagggcgac aggatgctcg gcggtggaag gctatgcgca
      481 tttacgcaaa ggggaagggg agttcgactt caagggcccg gagaagctgt attaaaattt
      541 aggctttaca ctcgcagccg aacaaaacgg cgttgcggtg atgcgcaaaa cactttagag
      601 aaatttacac atcaaaaaga aggccatccg gccttctttt tttgtatcgg aaggggttct
      661 taggggtgaa acccctgagc ggggtttggg gcagagcccc aagagaactt tcaccccagc
      721 gccacgtcga gaatcatcat gattaaaaac cccgccatca cgccgagcgt tccgatgttg
      781 gagtgctcgc ccaaatgcgc ctccgggatg agctcttcca cgaccacata catcatggcg
      841 cccgccgcaa aggagagcat ccagggcatg tacggcccga taaagccgga gacgaggacg
      901 gttaaaatgc cgaaaatcgg ctccacgatg ccggagagcg tgccgtagac aaacgacttg
      961 ccgacgctga acccttcctg ccggaggggc agcgaaattg ccgcgccttc gggaaagttc
     1021 tgtatgccga tgcccagcgc gagagcgagc gccgcggcat aggacgcggg tgtgccgccg
     1081 tgctgcgccg ccagcgcaaa cgaaaggccg acggccatgc cttccgggat gttgtggagc
     1141 gtcacggcca tcacgagcag cgacgtgcgc ttcatggacg tgctggggcc ttccgcatcg
     1201 gtcgagccgg gatgcaggtg cgggatgatc tggtcgagac ccagcaggaa caggatgcca
     1261 aggatgaagc cgcccgccgc cggaatccag ccgatcttgc cgttggcagc cgcttcctca
     1321 atggcaggaa ttaaaaggct ccaaacggag gctgcgatca tgacgcccgc ggcgaaaccg
     1381 aggaaaacct tttgtgactt tccgctcatc gacttgcgga agaaaaatac catggcggcg
     1441 ccgagcgtcg tcatcagaaa cgtaaagccc gttccggcgg acgccatcag caatgcttga
     1501 cccatcgatc gatccccgtc cttctgtaaa aaatttgttt gaattcaaga tcttagcgct
     1561 cgctgttcct tatttcggaa cagccaaaga gcgttcggtt agactaatct tacgatcaat
     1621 gacccgcttc gtcaagagcg gcggcgtata cagacaaagg agcggaccgc ccctgcgttc
     1681 cgccgaacgg ccgccccacc tactgacggc cgagagagac gaaacgcgcg gaagcggtcc
     1741 gaggagagag gatcatgagg gacacgggtc ttgctgtttc aaaagcaaac ggagcttttt
     1801 gtgagctccg ttttatcaac tagaattcga ggatatattg attcagcacg gtttccagat
     1861 attcctgacg gccggaagcg ttggggttcg tcccgtggat cagcgcatag gcttccagtt
     1921 cggagaaacc gaccctgccg ctgacgatgt ccttgccgat gccttccaag tagctttcgt
     1981 atcgcttggc cttaaagctt tccagcgtgc cgtcctccaa catctttgcc gcagcgcgga
     2041 ggcctttggc aaacgtatcc atgcccgcga tgtaggcaag gaagagatcg tcctcctgaa
     2101 aggaaccgcg gcgcaccttt gcgtcaaagt tcagcccgcc gctcgtaaag ccgcccgcaa
     2161 gcagaatttc gtacatggcg agggtggtgt cgtaaacgtt cgtcgggaac tggtcggtat
     2221 cccagccgag catgggatcg cccgtgttgg cgtccacgct gccgaatacg ccgttgatgc
     2281 gcgcggcgca gagttcgtgc tgaaaggtat gcgcggcgag cgttgcatgg ttggcctcga
     2341 tattcatctt gaagtggctt tcaaggccgt atttgcgcag aaacgctaaa accgtcgccg
     2401 tatcgaaatc gtattgatgc ttcgtgggct ctttgggttt gggttcgatc agaaactgcc
     2461 cggtaaagcc gatcttcttt gcgtaatcga ccgccatttt caagagccgc gcaaagttgt
     2521 ccagctccag ccccatatcg gtgttgagta gagtttcgta cccttcacgt ccgccccaga
     2581 agacatagtt ctcgccgccg agctccatgg taacctcgat ggcttttttg atctgtgcgg
     2641 cggtataggc aaacaccgtt gcgttggggg aggttgccgc gccgtggaca aatttcgcgt
     2701 ccccgaagca gttggaggta ccccagaggc atttgatgcc cgtgcgcgtc atctcctgct
     2761 tgacttctgc gaccacctcg tcgagaatgc ggttggtctc taaaagcgtg tccctgcgcg
     2821 gggcaagatc cgcgtcgtga aagcagaaat aaccaatctg catcttttcc atgaactcaa
     2881 atgccgcgtg cacgcgttcc ttggcgcgta tcacgtcgtt ttccgtttcg tcccatggtc
     2941 gaaagatggt cacgccgcca aacggatcga cgccgcggta ggtgaacgtg tgccaccagg
     3001 acatcgcaaa gcgcagatgc tccttcatgg gctttccggc cacgatttca tccgggttgt
     3061 aatacttgaa cgaaagcggg ttgacgctct ttgcgccttc gtagcggatg gtgcctattt
     3121 cactgaagta tgccatgtta ttcgctccta acggatattg cctgaaagcg ccgcgaaaat
     3181 cgggttacgc ggcaaccttg atcgctttgc gcttggatac gacgtcgaag ataaccgccg
     3241 ccagcagcac aaggcctttg acgaccttct ggaagttcga gtccacgccg atgatgttca
     3301 tgccgaggtt gagaacgccc atgagcaccg cgccgatgac cgccccgctg accttgccga
     3361 tgccgccgta agcggatgcg ccgccgatga agcaggagcc gatggcgtcg agctcgtagt
     3421 tcgtgccagc ggcggggttg gcggaggtaa agcgggcggc gactacgaga gccgccaagg
     3481 cgctcaggaa cgaaacgttg acatacgccg agaagtaaat gcggtttgtg ttgatgccgg
     3541 agagctttgt ggccttttcg ttgccgccca gcgcgtagaa atggcggcca aaggtggtct
     3601 tcgcggtgat gtaggtatag atggccacga tgccgatgag ccagagcagg atgtacggaa
     3661 tgcccttata gagcgagagc ttatacatca gcagcatgac gacgccgcag atgatgacaa
     3721 gccgcgtgta gagggaagcg cggttttcca cggcgtatcc ttttttcttt ttgttgatgt
     3781 tgccgaagat ggtgagcacc acgtatacca cgcagcagac ggcgccaaag atgatgctgc
     3841 tgatcttcat a
//
LOCUS       DAOMGY010000060         3821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_24936_length_3821_cov_2.536909, whole genome shotgun sequence.
ACCESSION   DAOMGY010000060 DAOMGY010000000
VERSION     DAOMGY010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24936_length_3821_cov_2.536909"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLR57_01600"
     CDS             <1..373
                     /locus_tag="PLR57_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40178.1"
                     /translation="MPQGYIQNNPNMPPYRDAIEAGVLPAYRGYQMTRDDHIRADVIE
                     KIMCYFEIDLKKISEKYHVPLSYFDNSIASVIKTLPDGTYELCNSVLRLTVMPHLTAR
                     LCAHAFDAHAVHIENRHARAI"
     gene            complement(388..2781)
                     /locus_tag="PLR57_01605"
     CDS             complement(388..2781)
                     /locus_tag="PLR57_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQQ40179.1"
                     /translation="MEAVNMEAVELNSQLLEVAYRIREMREISGFTREEMAKKTDVTP
                     AEYAAYESGRSDFPFTFIHKCALAFGIGMSDLLEGDSARLSSYTVTRKGQGQPAAKEE
                     GIEINSLAPMFRNKIAEPYWVRYDYSETQQNRPIHLTTHNGQEFDLVLSGSLKVQIGE
                     HVEVLNEGDSIYYNSATPHGMLAVGGKECLFVAVVLPGEGAENAAELHASIVEARKAE
                     DFVANRFIDAREDAQGQLSGVSFPNADQFNFAFDIVDAIAKKSPDKLAMLHVDYNGVE
                     RRFSFEDMMKSSNRCANYFLSLGIQKGDKVMLVLKRHYQFWFAMLALHKIGAVAIPAT
                     YQLVAHDFTYRYQAADVSAIVCTADGDVAAQAELGEEGCTPLKARVLVGGSREGWHSF
                     DEEYARFSSRFERPEDAIQGDDIMLMFFTSGTTGYPKIASHSCKYALGHFVTAKYWHC
                     VHSDGLHLTVSDTGWGKALWGKFYGQWLCEGAVATFEFERFDAKYLLPLLGKYQVTTL
                     CAPPTIYRMMIKEDLTQYDFSTIVHATTAGEALNPEVFYQFEQATGLRIMEGFGQTET
                     TLLIACLAGMTPKPGSMGKPVPTYDIDLLTPEGKRAELGEPGEIVIRLDNGNPCGLYQ
                     GYYRDEEQTNESCADGVYHTGDLAWRDEDGYFWYVGRKDDVIKSSGYRIGPFEIENVI
                     MELPYVLECGVSGAPDEIRGQVIKASIVLTKGTEPTDALKTEIQEYVKTHTAPYKYPR
                     IVVFREELPKTISGKIMRRILRKIASMETEHLGDTSTLADPDIVPQLVKGRSDITLP"
     gene            complement(3287..>3821)
                     /locus_tag="PLR57_01610"
     CDS             complement(3287..>3821)
                     /locus_tag="PLR57_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQQ40180.1"
                     /translation="HAPQGVSTAVNGEFVARGYNIMKGYYKMPGATRSAIDREGWLHT
                     GDLACKTPEGNYRITGRLKDMIIRGGENIYPKEIEEFLYTHPKIKDVQVIGVPDAQYG
                     EEIMACVILKEGETATQEEIQSFVRASMAKHKVPRYVEMVDSFPMNAAGKILKYKMRE
                     DAIAKLKLQKAASIETA"
BASE COUNT          737 a         1114 c         1034 g          936 t
ORIGIN      
        1 catgccgcaa ggttatattc agaataatcc aaacatgccg ccttatcgcg atgcgatcga
       61 ggctggcgta ttacccgcat atcgtggtta tcaaatgacc cgcgatgatc atattcgcgc
      121 cgatgttatt gaaaaaatca tgtgttattt tgagattgac ctcaagaaga tttcagaaaa
      181 atatcatgtc ccgttatctt attttgataa cagcattgca tctgttatca aaacattgcc
      241 ggatggcaca tacgaacttt gcaattctgt tttaagactg actgtcatgc cgcatttaac
      301 ggcgcgcctt tgtgcccatg cgtttgatgc acacgccgtt catatagaaa acaggcatgc
      361 ccgggcgata taaattcatc tcaatcctta tggcagtgtt atatccgatc gccccttgac
      421 caactgcgga acaatatccg gatccgctaa tgtcgaggta tcacccaaat gttctgtttc
      481 catcgaagca atcttacgca agatccggcg cataatcttt ccggaaatgg tcttaggcag
      541 ttcttcgcgg aagaccacga tgcgcgggta tttatacggc gcggtatgcg ttttcacgta
      601 ctcctgaatc tccgttttga gcgcgtcggt gggctcggtg cccttggtca gcacgatgct
      661 ggccttgatg acctgcccgc ggatttcgtc cggcgcgccg cttactccgc attcgagcac
      721 gtacggcagt tccataatga cgttttcaat ctcgaaaggt ccaatgcggt agccggagga
      781 tttgatcacg tcgtcctttc ggcccacgta ccagaagtag ccgtcctcat cccgccaggc
      841 gagatcgccc gtgtggtaca cgccgtctgc gcaggattca ttcgtctgct cctcgtcccg
      901 gtagtaaccc tgatacagtc cgcagggatt gccgttgtcg agccggatga cgatttcgcc
      961 cggttcgccg agctcggctc gcttgccttc cggcgtgagc agatcgatat cgtacgtcgg
     1021 cacaggtttg cccatggagc ccggcttggg cgtcataccc gcaaggcacg caattaaaag
     1081 cgtcgtctcg gtctggccga agccctccat aattctcagc ccagtggcct gctcgaactg
     1141 atagaacact tcggggttga gcgcttcgcc cgccgtcgtg gcatggacga tggtggaaaa
     1201 gtcatactgc gtgagatctt ccttgatcat catgcggtag atcgtcggcg gcgcgcagag
     1261 cgtcgttacc tgatattttc ccagcagcgg caggaggtat tttgcgtcga agcgctcaaa
     1321 ttcgaacgta gccaccgcgc cttcgcagag ccattgcccg taaaacttgc cccagagcgc
     1381 cttgccccag ccggtatcgg aaacggttag gtgcagtccg tcggaatgga cgcagtgcca
     1441 gtactttgcg gtcacgaaat gcccgagcgc gtatttgcag ctgtgggagg cgattttggg
     1501 gtagcccgtc gtgccggagg taaagaacat cagcatgata tcgtcgccct gaatcgcgtc
     1561 ctcggggcgc tcaaagcggc tgctgaagcg cgcatactcc tcgtcgaagc tatgccagcc
     1621 ttcgcggctt ccgccgacga gaacgcgggc cttcagcggc gtgcagcctt cctcgccgag
     1681 ttccgcctgc gcggcaacgt cgccgtccgc cgtgcagacg atggcggaaa catccgcggc
     1741 ctgatagcgg tacgtaaagt cgtgcgcaac gagctgatag gttgccggaa tggccaccgc
     1801 gccaatttta tggagcgcaa gcattgcaaa ccagaactgg taatggcgct tgagcaccag
     1861 catgaccttg tcgcccttct ggatgccgag cgagagaaag tagttggcgc agcggttgga
     1921 ggacttcatc atgtcctcaa acgagaagcg gcgctccact ccgttgtagt ccacatgcag
     1981 catggcgagc ttgtcgggcg atttcttcgc gatggcgtcc acaatgtcga acgcaaagtt
     2041 gaactgatcc gcgttcggga aactaacgcc ggagagctgg ccctgcgcgt cctcgcgcgc
     2101 gtcgataaag cggttggcaa cgaagtcctc ggcctttctc gcttccacga tgctcgcgtg
     2161 cagttccgcg gcgttctccg cgccttcgcc cggcagtacg accgcaacga acaaacactc
     2221 tttgccgcct acggcgagca tgccgtgggg agtagcactg ttgtagtaga tgctgtcgcc
     2281 ttcgttcaac acttcgacat gctcgccgat ctgcactttg agtgagccgc tgagcacaag
     2341 gtcgaactcc tgaccgttgt gcgtggtcag gtggatgggg cggttctgct gcgtttccga
     2401 gtagtcgtac cgcacccagt acggctcggc gatcttattg cggaacatcg gagcgaggct
     2461 gttgatttca atgccttcct ccttggcggc gggctggccc tggcctttcc gcgtcaccgt
     2521 gtaggaagag agccgcgcgc tgtcgccttc cagcaaatcg gacatgccga tgccgaacgc
     2581 gagcgcgcac ttatggataa acgtaaacgg aaagtccgat ctgcccgatt cgtaagccgc
     2641 gtactcggcg ggggttacgt cggttttttt cgccatctcc tcgcgggtaa agccggagat
     2701 ttcccgcatc tcgcgaattc ggtatgcaac ctccaacagc tgcgagttca gttctaccgc
     2761 ttccatattt accgcttcca ttgcgcctcc ttgccggcag attggctgcc gaaccatatt
     2821 gttttctcgc ggacctcgtc cgcacctgac ttacataatc tttcgcggca ataaaaaaca
     2881 cccgccccaa gattttcgtc ttgagacggg tgatcgtttt gatctgcccg cggtaccact
     2941 caaattgcgc ttttacgcgc ctctcatcag gctcctataa gccctttgcc ttcacgcggc
     3001 aactacggaa agctctactt gcggattccc cgctttggag ttttcggctc ggaagggatg
     3061 ggactgattg aaagttgccg ccgccgggat ttcaccctcc ccgactctct ttgtgcgctg
     3121 gctttcgtcc gtcttcgtca cagcctttaa aacgttattt agttgtcact cgcttgaccg
     3181 atttcagtac tctcgtacgc gtatcgacca attgcattgt atattatcag ccgcgcatgg
     3241 ctttgtcaac gctaactttt cagtatttta acagcgtgtc cgccggttac gcggtttcga
     3301 tgctcgcggc tttctggagc ttgagcttgg caatcgcgtc ctcgcgcatc ttgtatttga
     3361 ggattttccc cgccgcgttc atcgggaaag aatccaccat ttccacatag cgcggcacct
     3421 tgtgcttggc catgctggcg cggacgaacg attgaatctc ttcctgcgtc gccgtttccc
     3481 cttccttgag gataacgcag gccataatct cctcgccgta ctgcgcgtcc ggcacgccga
     3541 tgacctgcac gtccttgatc tttggatgcg tgtagaggaa ctcctcgatc tccttggggt
     3601 aaatattttc gccgccgcgg atgatcatgt ccttgagccg accggtgatg cggtagttcc
     3661 cttccggcgt tttgcaggca aggtcgcccg tatgcagcca gccttcgcgg tcgatggcgg
     3721 agcgggttgc tcccggcatc ttatagtacc ccttcatgat gttgtaaccg cgcgccacga
     3781 actcgccgtt gacggcggtg ctgacgccct gcggcgcatg g
//
LOCUS       DAOMGY010000061         3817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_24976_length_3817_cov_3.061669, whole genome shotgun sequence.
ACCESSION   DAOMGY010000061 DAOMGY010000000
VERSION     DAOMGY010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24976_length_3817_cov_3.061669"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="PLR57_01615"
     CDS             <1..1208
                     /locus_tag="PLR57_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40181.1"
                     /translation="IADSLACTCTIIGQDCYYRNFPDLPFEKRIKLNYDEPEIFDFEE
                     MFRDVEKLLHGEPITTKGYDYTNYLRADSPDVLIQPPEVLILEGIHMFYDKRICDLMS
                     LKVYLHVDVDVCLLRRIKRDIKVRGRNIDNIAEQYMETVKPVYEKYIEGYINDADFAV
                     MRGGKNKMAIDAISAYLTTKVLAERFGTEAPATAIKDKPASAEPDDLPDAPVEPKANE
                     KKPDSSDAEIEGDDPFDFLVSDKRDSSAGDGEGDNPSECEEYENEDDVDYVPSRYARH
                     RRRVARKTLRLIFFSTFLNFGLAIFALFMLGQVYNHDWAMTQSGGMNAIIDALVQVFF
                     LVLPILLMALFNRLLYKMFDLDYSPGWLIFLLVLWILIVLGGTLYLVYAFNLLDGMAG
                     FGLGGLFGA"
     gene            1233..1673
                     /locus_tag="PLR57_01620"
     CDS             1233..1673
                     /locus_tag="PLR57_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40182.1"
                     /translation="MESKFHRSSDEPQIKKHGAGRVTVFFLTLVIINVLLGIYTLYML
                     GFALAYDWTQLKTGSVQAILEAALQLLLLLFSPVILTLIINRLLFRAFRGHGRFPRGV
                     WFFAILAILAVQAATMYGILSFGFVDGINGFSVDSLAELRFPAN"
     gene            1828..2601
                     /gene="surE"
                     /locus_tag="PLR57_01625"
     CDS             1828..2601
                     /gene="surE"
                     /locus_tag="PLR57_01625"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQQ40183.1"
                     /translation="MKLLLSNDDGVFASGIRVLAETLSKTHEIYVSAPDAERSGVSRS
                     MTLFEPLRAKPTKIEGLPEIPAYAVSGTPVDCVRLALGNLFTAPDLVVSGINLGPNLG
                     TDVLYSGTVAAAHEAALLGYQAIAISSLSYKGEFLETAARVAEIAVEYVRDHPMDFGT
                     LLNINVPGIPYEELKGIKVTSACVEQYELEFIEREDPLGRKYYWPPHGRTSCSDGLDV
                     DDRWAREGYVTVTPLMYDLTDYPRLRNMQLSLPDWIANR"
     gene            2651..3523
                     /locus_tag="PLR57_01630"
     CDS             2651..3523
                     /locus_tag="PLR57_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HQQ40184.1"
                     /translation="MDALFSLMEKQYPTFSKGQRRIAEYILNSYDDAAFMTAAKLGER
                     VGVSESTVVRFAYMLGLDGYPALQEALQDLIRHRLTSVQRIRLAANIPQQDVLKTVLT
                     SDMNNIRTTIDMIDNESFEGAISATLRARRIYVLGIRSAMSLAQFLTYYLDYVCDNVM
                     FVNGAVQDIHERMLRVGPQDVCFGISFPRYSARTVDAMKYAKSKGATLIALTDMPTSP
                     LAKISDYTLCARSDMASFADSLVAPLSLINAIIVAVGHARKDEATQHLTQLEDIWHAE
                     GVYLSETIKEEKPS"
     gene            3520..>3817
                     /locus_tag="PLR57_01635"
     CDS             3520..>3817
                     /locus_tag="PLR57_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQQ40185.1"
                     /translation="MIVFVIGGGPAGMMSALFAAQNGHEVTLFERNEKLGKKLYITGK
                     GRCNVTNAADRDTFFAHILQNPRFLYSAYSHFDNQALMERLERAGVPLKTERGGR"
BASE COUNT          846 a         1039 c         1066 g          866 t
ORIGIN      
        1 gaatcgcgga ttcgcttgcc tgcacctgca cgatcatcgg gcaggactgc tactatcgca
       61 attttcccga tctgccattc gaaaagcgaa tcaaactcaa ctacgacgag ccggaaatct
      121 tcgattttga ggagatgttc cgcgacgtgg aaaaactcct ccacggtgaa ccgatcacaa
      181 caaagggata cgattacaca aactacctgc gcgcggattc gccggacgtt ctgattcagc
      241 cgccggaagt gctgattctc gaaggcattc acatgtttta cgacaagcgc atctgcgatc
      301 tgatgtcgct caaggtctac ctgcatgtgg acgtggacgt ctgcctgctc agacgtatta
      361 agcgcgatat caaggtgcgc gggcgtaaca tagacaacat tgccgagcag tatatggaga
      421 ctgtgaagcc cgtatacgaa aagtacatcg aagggtatat caacgacgcg gatttcgcgg
      481 ttatgcgcgg cggcaaaaac aagatggcca tcgacgccat ctccgcctac ctgactacga
      541 aagtgcttgc cgaacgtttc ggcacggaag cgcctgccac tgccatcaag gataagcctg
      601 catccgcgga accggacgat ctgccggatg cgccggttga accgaaggcg aacgaaaaga
      661 aaccggactc ttcggacgcc gaaatcgagg gcgacgaccc ttttgacttc ctggtttccg
      721 acaaacggga ttcctctgcc ggcgacggag aaggcgacaa tccttcggaa tgcgaagagt
      781 atgaaaacga ggatgacgtc gactatgtcc caagccgtta cgccagacat cgccgccgcg
      841 ttgcaagaaa aactctgaga ttaatctttt tttcgacgtt tctgaatttc ggcctcgcca
      901 tctttgcatt gttcatgcta ggtcaggtgt ataatcacga ctgggcgatg acgcagagcg
      961 gagggatgaa cgcgattatc gatgcactcg tgcaggtgtt tttcctcgta ctgccgatcc
     1021 tgctcatggc gcttttcaac cgcctgcttt ataaaatgtt cgatctcgat tacagccccg
     1081 gttggctcat tttcctgctc gtgctctgga ttcttattgt gctgggcggc acgctctatc
     1141 tcgtttacgc cttcaacctg ctggacggga tggcggggtt cgggctcggc ggcttatttg
     1201 gcgcataatg cataagaaag ggactgcctt ccatggaatc aaaatttcac cgctcttctg
     1261 acgaaccgca gatcaaaaaa cacggcgcag gacgcgttac cgtatttttc ttaacgctcg
     1321 tcattatcaa cgttttgctc ggcatctaca cgctgtatat gctcgggttt gcgcttgcgt
     1381 atgactggac gcagcttaag accggcagcg tgcaggccat tctggaagcg gcgcttcagc
     1441 tgctgctgct gctgttctcg ccggtgattc ttacgctgat tatcaaccgc ctgctgtttc
     1501 gcgcatttcg cgggcatgga cgctttccgc gcggggtctg gttctttgcc atccttgcga
     1561 ttctcgcggt gcaggcggcg accatgtacg gcattctcag cttcgggttt gtggacggaa
     1621 tcaacggctt cagcgtcgat tcactcgcag agcttcgttt tcctgcaaac tgacgctgtc
     1681 caattacggc attccagctc attttctctg aatcgtacat aaatcatgtg caggagaact
     1741 gtatttttcc tgcacattct tgtttttatg gcattcaacg cacaggtatg atagaataac
     1801 tttacattta tttgacggag ttcctgcatg aagctacttt taagcaatga cgacggcgtt
     1861 ttcgcgagcg gtattcgcgt tctggccgaa acgctctcaa aaacccatga aatatacgtt
     1921 tccgcgccgg acgccgagcg cagcggggtc agccgctcca tgacgctgtt cgaaccgctt
     1981 cgcgccaagc cgacgaaaat cgaaggcctt ccggagatac cggcctatgc cgtgagcggc
     2041 acgccggtgg actgcgttcg tcttgcgctg ggaaatctgt ttaccgcgcc tgatctcgtc
     2101 gtctccggca tcaatttagg gccgaatctt ggcacggacg tgctctacag cggcacggtc
     2161 gcggcggcgc acgaagcggc gctgctgggg tatcaggcca tcgcaatcag ctcgctttcg
     2221 tacaaaggcg aatttctgga aaccgccgcg cgcgttgccg aaattgcggt agagtatgtc
     2281 cgcgatcacc cgatggattt tggcacgctg ctcaatatca acgtgccggg cataccctat
     2341 gaagaactca aaggaattaa ggtcacgtcc gcctgcgtgg agcagtatga actggagttt
     2401 atcgagcggg aagacccgct tggaagaaaa tactactggc cgccgcacgg aagaacctcc
     2461 tgctcggacg gactggacgt tgacgaccgc tgggcgaggg aagggtatgt aaccgtcacg
     2521 ccgctgatgt acgatcttac ggactatccg cggcttagga atatgcagct gtcgctgccg
     2581 gactggattg caaacaggta aaggtgagta aaaagagata aaataaacga tcgaggagga
     2641 gcaaagcgga atggacgcat tgttttcgct gatggaaaaa cagtacccaa cgttttccaa
     2701 ggggcagaga aggattgcgg agtatatcct gaattcctat gacgacgcgg cgttcatgac
     2761 ggctgcaaag ctgggcgagc gcgtcggcgt ttcggaatcc acggtcgtcc gttttgcgta
     2821 tatgcttggc ctcgacgggt atcctgcgct gcaggaagcc ctccaggatt taatccggca
     2881 ccggctcacg agcgttcagc gcattcgcct tgccgccaat attccgcaac aggacgtgct
     2941 gaaaaccgtt ctgacgtcgg atatgaacaa cattcgcacc acgatcgaca tgatcgacaa
     3001 cgagagcttc gagggcgcga tcagcgcgac gctccgcgcg cggcgcatct atgtgctggg
     3061 cattcgcagc gccatgtcgc tggcgcagtt tttgacctat tacctcgact atgtctgcga
     3121 caacgtgatg ttcgtcaacg gcgcggtgca ggatatccac gagcgcatgc tgcgtgtggg
     3181 tccgcaggac gtctgcttcg gcatcagctt tccgcgctac agcgcgcgca ctgtggacgc
     3241 tatgaagtac gcaaaatcca agggcgcgac gctgattgcg ctcaccgata tgccgacctc
     3301 gccgctggca aagatttcgg actatacgct ttgcgcgcgc agcgatatgg cctcgtttgc
     3361 ggactcgctg gtggcgccgc tttcgctgat caacgccatt atcgttgccg tcggccatgc
     3421 gcgcaaggac gaggcaaccc agcacttaac gcagttggag gatatctggc acgcggaggg
     3481 cgtttacctt tcggaaacga ttaaagagga gaagccctca tgatcgtctt cgtcatcggc
     3541 ggcggccctg cgggcatgat gagcgcgctg ttcgcagctc agaacgggca tgaggttacg
     3601 ctctttgagc gcaacgaaaa gctcggcaaa aagctctata tcaccggcaa aggccgctgc
     3661 aacgtgacca acgccgccga cagggacacg ttctttgcgc atattctgca aaacccgcgc
     3721 tttctctaca gcgcgtattc gcattttgac aatcaggctt tgatggaacg gctggaacgc
     3781 gcgggcgtac cgctcaaaac ggagcgcggc gggcggg
//
LOCUS       DAOMGY010000062         3812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_25030_length_3812_cov_2.754059, whole genome shotgun sequence.
ACCESSION   DAOMGY010000062 DAOMGY010000000
VERSION     DAOMGY010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25030_length_3812_cov_2.754059"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1058)
                     /locus_tag="PLR57_01640"
     CDS             complement(90..1058)
                     /locus_tag="PLR57_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40186.1"
                     /translation="MKSVFSSAVQDFKRLLTNALFWVLTGTLAVIVLVVDLALPKELA
                     QQEYRLVTYSAPAAMTAGEPAESLDALKEAVRQGDAIGVAFGEDGITIVHPGYSQKTL
                     NAMVLELSGTQAEPVNTQLLEADAKTIPFNFRLTPIFICFEALMVGFILGGALMLAEK
                     QDGTAKALRILPFGAGRYIVSKTLLFSLVGTLYAALICVLTVGFQINWGLFLLLSFFG
                     TAVFTMIGLAYTSPFHDMSGWFFSMVVLLSVNMLPVISYSSPSFTPVWMKLIPSYPIL
                     MAYRSAMFGGTIEWGYTLLSIGAWGVLSYLLAQVFVQRNHLKGVKA"
     gene            complement(1055..1903)
                     /locus_tag="PLR57_01645"
     CDS             complement(1055..1903)
                     /locus_tag="PLR57_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40187.1"
                     /translation="MIQVNNVFHDYEGKGNCAVSDISFTVEGGSIFGFLGPSGAGKST
                     IQNIMTGLLPLQRGEVLYDGASVRGLKTRFFNQIGVSFEQSNLYSSLTGLENLRYYAA
                     LFSVPTIKPMELLERVGLREDANKRVSNYSKGMKQRLTFARALINSPKYLFLDEPTGG
                     LDPKTAVGIRELINEQKQRGAAVFLTTHSMDLADALSDTVAFLYGGVIAACDTPEALK
                     LKYGKRDVRVRYNENGEEKERTFAMDDKAALSAFLTTADVVKLDSQEATLEEIFLLVT
                     GKELTA"
     gene            complement(2163..2588)
                     /locus_tag="PLR57_01650"
     CDS             complement(2163..2588)
                     /locus_tag="PLR57_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40188.1"
                     /translation="MKQQILSQIESKLGAIGVIAKPDVKTDLFVEAELLDASFSTGKK
                     KIRYEAAILADEADKTVRMYEKTTEISAGVSFGFSGESTMQSGRTLFRKVMSVQYGPD
                     GKAYEYTFDLGAIAKTVKYAAEQNGWKFKTVILKKKAMY"
     gene            complement(2839..>3812)
                     /locus_tag="PLR57_01655"
     CDS             complement(2839..>3812)
                     /locus_tag="PLR57_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HQQ40189.1"
                     /translation="LRGRLGELDKSKPTVVFCAIGVRAYNGARILQQNGFGTVSVYPG
                     GSRFYQTTHFKEENTVPYTQNAPVSDSGHADTRNVPVSSMRLDCSGMQCPGPIMKVFE
                     TMKEMKDGDVMEVSASDPGFARDIVAWTRRTGNTLLSNERRGGDYVALVKKGLAGGAP
                     AAVAQANGDGKTIIVFSGDLDKVLASFIIANGAAAMGRKVTMFFTFWGLNVLRKNNRQ
                     KVDKSFIEAMFGGMMPRGVNKLKLSKMNMMGMGTSMMKMIMKKKNVDSLETLMKKAMA
                     AGVKIIACTMSMDVMGIKEQELIDGIELGGVGAYLGDAEESNVNLFI"
BASE COUNT          780 a         1139 c         1036 g          857 t
ORIGIN      
        1 aaggatggcg ggcgcggcaa ccatgaaaat ggagatgccg ttgcggaaga tgagtttaaa
       61 ctccctgccg attgaggtaa acagcgtctt catgctttca cccccttcag gtgatttctc
      121 tgcacaaaca cttgcgcaag aaggtacgaa agaacgcccc atgcgccgat ggaaagcagg
      181 gtatatcccc actcaatcgt cccgccgaac atggcgctgc ggtacgccat caagattgga
      241 tacgaaggaa tcagcttcat ccagacaggc gtaaacgaag gcgaggagta gctgataacc
      301 ggcagcatgt tcacgctcaa cagcaccacc atagagaaga accatccgct catatcgtga
      361 aacggcgagg tatacgccag cccgatcatt gtaaacaccg ccgtgccgaa gaacgaaagc
      421 aacaggaaca gcccccagtt gatctggaac ccgaccgtca gcacgcagat cagcgcagcg
      481 taaagcgtgc cgaccagcga aaacagcagc gttttggaaa cgatgtaccg gcccgcgcca
      541 aacggtaaaa tccgcagcgc ctttgccgtg ccgtcctgct tttccgccag catcagcgcg
      601 ccgcctagga taaagccgac catcagcgct tcaaaacaga taaagatcgg ggtgagccgg
      661 aagttgaagg gaatcgtctt tgcgtccgct tccagcagtt gcgtgttcac cggctctgcc
      721 tgcgtgccgg agagctccag caccatggcg ttaagcgttt tttgcgagta gccggggtga
      781 acgatggtaa tgccgtcctc cccgaatgca acgccgatgg cgtcgccctg ccgcacggct
      841 tccttgagcg cgtccaggct ttccgccggc tcgccggccg tcattgccgc gggcgcgctg
      901 taggtaacca gccggtattc ctgctgcgca agctctttgg gcaacgcaag gtcaaccaca
      961 agcacgatca ccgccagcgt acccgtaagc acccagaaca gcgcgttggt cagcaggcgc
     1021 ttaaagtcct gcaccgcgga actgaatacg cttttcacgc cgtcagctcc tttccggtca
     1081 caagcagaaa gatctcctcc agcgtcgctt cctgcgagtc gagtttgacc acgtccgccg
     1141 tggtaagaaa cgcggagagc gcagctttgt cgtccatggc gaacgtccgc tccttctcct
     1201 cgccgttttc gttgtagcgc acgcgtacgt cccgcttgcc gtacttgagc ttaagcgctt
     1261 ccggcgtgtc gcaggcggcg atcactccgc cgtacagaaa cgccaccgtg tcgctcagcg
     1321 cgtcggccag gtccatgctg tgggtggtca aaaataccgc cgcaccccgc tgcttctgct
     1381 cgttgatgag ctcgcggatg ccgacagccg tcttgggatc gagcccgccg gtcggttcgt
     1441 ccagaaacag gtatttgggg ctgttgatca gcgcgcgcgc aaatgtaagc cgctgcttca
     1501 tgccttttga gtagttggaa acgcgtttgt ttgcgtcctc gcgcagaccg acgcgttcga
     1561 gcagctccat gggtttaatc gtcggcaccg aaaacagcgc cgcgtaatag cggagattct
     1621 ccagccccgt cagtgaggaa tagaggttgc tctgctcaaa cgaaacgccg atctggttaa
     1681 agaaacgcgt tttcagcccg cgcacggacg cgccgtcgta gagcacttcc cccctctgca
     1741 gcggcagcag ccccgtcatg atgttttgaa tcgtagactt tcccgcaccg gaggggccga
     1801 gaaagccgaa gatgctgccg ccctctacgg taaaggaaat gtccgaaacg gcgcagttgc
     1861 ccttaccctc gtagtcgtga aacacgttgt tgacctgaat catagccttc ctccttgacc
     1921 aagtagtcaa gtagaatgta ccacgatttg accatccggt caagaacttt ttgaataatg
     1981 aaacccactc gccgttttgc gtctgtccaa aacgaaaaac cggcggaaga tcactcctcc
     2041 gccggtcttg ttttcgctcc gctgacacgc gcagtgacgc gcgtatgtcc ttgcggtttt
     2101 cgctgaacct gtattcctgc cggattgctc cggctgattg actcagtaac gcagaagcct
     2161 gcttagtaca tcgctttctt tttcaggatg accgttttaa acttccagcc gttctgctcg
     2221 gcggcgtact tgacggtctt cgcaatcgca ccgagatcga aggtatattc gtacgccttg
     2281 ccgtccggcc cgtactgcac gctcatgacc ttgcgaaaca gcgtccttcc gctctgcatg
     2341 gtggactccc cgctgaagcc aaaggagacc ccagcgctga tctcggtcgt cttttcgtac
     2401 atgcgcaccg ttttatccgc ttcgtccgcc aggatggcgg cctcatagcg aattttcttt
     2461 ttgccggtgc tgaagctggc gtccagcagt tccgcctcca caaagagatc ggtctttacg
     2521 tccggttttg caataacgcc aatcgctccc agcttggatt cgatttgcga gagtatctgt
     2581 tgtttcataa ttgatgcctc cctgtcaaaa cgatttgcaa cgctgagcag gaacgtatgt
     2641 tcctgttgtg aggctagtat aacactcgtg gtttgccttg tccagaaaat tgttacatag
     2701 taatttatag gtaaaaaaca tccgtctttc actgcggcgc agacggacga taggctcgaa
     2761 atccggtaaa aagcgccgca gcgggcgggt gtttcccgtg cgctgcggcg tcttgttgct
     2821 tgtgttattt gacctcagtt aaatgaagag gttgacgttg gactcttccg cgtcgcccaa
     2881 gtatgcgccc acgccgccga gttcgatgcc gtcgatcagt tcctgctcct tgatgcccat
     2941 aacgtccatg ctcatggtgc aggcaataat cttgacgcct gcggccatgg ccttcttcat
     3001 gagcgtttcg agggaatcga cgttcttctt cttcatgatc atcttcatca tggacgtgcc
     3061 catgcccatc atgttcatct tgctgagctt gagtttgttc acgccgcgcg gcatcatgcc
     3121 gccgaacatg gcctcgatga aggacttgtc cactttttgc ctgttgttct ttctgaggac
     3181 gttgaggccc cagaaggtga agaacatggt cactttgcgg cccattgctg ccgcgccgtt
     3241 ggcgatgata aagctcgcca gcaccttgtc cagatcgccg gagaagacga tgatggtctt
     3301 tccatcgccg tttgcctgcg caaccgccgc cggtgcgccg cccgcgagac ctttcttaac
     3361 gagcgccaca tagtcgccgc cgcgccgttc gttgcttaag agcgtgttgc ccgtgcgccg
     3421 cgtccaggcc acgatatccc gcgcaaagcc ggggtcgctg gcggagactt ccataacgtc
     3481 gccgtccttc atttccttca tggtctcaaa gaccttcatg atcgggccgg ggcactgcat
     3541 gccgctgcag tcgagccgca tggacgaaac cggcacgttg cgggtatccg catgcccgct
     3601 gtccgaaacg ggcgcgttct gcgtgtaagg cacagtattc tcctccttaa agtgcgttgt
     3661 ctggtaaaag cgcgagccgc cgggatacac ggaaaccgta ccgaatccgt tctgctgcag
     3721 gatgcgcgcg ccgttgtagg cgcgaacgcc gatggcgcag aagaccacgg tgggtttgct
     3781 cttgtcgagt tcgccgaggc gtccgcggag ct
//
LOCUS       DAOMGY010000063         3807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_25066_length_3807_cov_3.103678, whole genome shotgun sequence.
ACCESSION   DAOMGY010000063 DAOMGY010000000
VERSION     DAOMGY010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25066_length_3807_cov_3.103678"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..1294
                     /gene="galE"
                     /locus_tag="PLR57_01660"
     CDS             281..1294
                     /gene="galE"
                     /locus_tag="PLR57_01660"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQQ40190.1"
                     /translation="MRILVTGGAGYIGSHTVLELLAAGYDVLAVDNFANSKPAAVRRT
                     GELAAKPFAFERCELTNADEVEALFASNKIDAVIHFAGLKAVGESVAKPLEYYRNNLV
                     STLNLLFAMRAHGVNQFVFSSSATVYGDPASVPVREDFPLSATNPYGQTKLMIELMLR
                     DAVIADPNLRIALLRYFNPVGAHVSGRIGEDPNGIPNNLVPYIAQVAVGKLPALRVFG
                     ADYPTPDGTGVRDYIHVVDLARGHVAALKKLQSGAGVYTYNLGTGVGYSVLDMLHAFE
                     KACGKTLAYEVVERRPGDIASCYADPTKAAEELGWRAEYGVERMCADTWRWQSQNPNG
                     FED"
     gene            1430..2356
                     /locus_tag="PLR57_01665"
     CDS             1430..2356
                     /locus_tag="PLR57_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQQ40191.1"
                     /translation="MQKPTLVVMAAGMGSRYGGLKQIDPVGRHGEIIMDYSLFDAMQA
                     GFEKVVFIINRKIEHDFYEVIGRRAEKHLEVHYVFQQPEAMLPAGFAVPEERVKPWGT
                     AHAILCCKDVVHEPFAAINADDYYGKRAFSVLFDYLKSARDGEKYDFSMVGYEAQNTL
                     TENGHVARGVCEVNDKGELVNIVERLRIYKTPEGPAYTEDEGKTFVHFPADNLVSMNF
                     FGFTPSLFGELEARFPQFLEAALRDNPLKGEFLIPQEVGLLLQKGKASVRVLTSPDRW
                     YGVTYREDKPEVERVLSEMTLSGLYPDEKLIG"
     gene            complement(2469..2621)
                     /locus_tag="PLR57_01670"
     CDS             complement(2469..2621)
                     /locus_tag="PLR57_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40192.1"
                     /translation="MYTIKNEFLMEEPLFASRPLGLAGGKEHRETARNGRSVAPDEPA
                     QPECMG"
     gene            complement(2711..2875)
                     /locus_tag="PLR57_01675"
     CDS             complement(2711..2875)
                     /locus_tag="PLR57_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40193.1"
                     /translation="MKKTMIVLMLAVSAMFLFAACNPTGAASGNNVSGGNSFVSGGNI
                     LPAVSAGNAN"
     gene            3026..>3807
                     /locus_tag="PLR57_01680"
     CDS             3026..>3807
                     /locus_tag="PLR57_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40194.1"
                     /translation="MTMEPYQLQQLRIRRFSTNVYLALVCFGMPLIFTRFYFNITETK
                     HVFFLTCSLVYALALVLIAILFPAGYGVAKPERKPVAPAIWLLIAFFGCLVLGGVLGG
                     HPEDAFFGQNNRYQGILTTFTYCVVVFALSRQTLDLRFAEAATAAGAALVGALGLLNH
                     YGIDPFGFMTKLTEFDRGRFLSTIGNADFYGSYFSLAFPVTFGLFLHAERKHTRALSV
                     SALVLASFGALVAGSDSTALGMIAAAVVFPLVLFGDPRGFRR"
BASE COUNT          795 a         1112 c         1051 g          849 t
ORIGIN      
        1 tgtccggtat aagccaagct gaattatacc atagcaatat tatttaggaa agctaaaaat
       61 gctccaaaat tgaagaaatc cgtatttttt tatcgattat gccatattta ttaacggctt
      121 tgacagccat agcgcctcgc gcctataata gaggcagcaa cgcgcccgct tttttgcggc
      181 agccgcccga aaatccgttt ggcgcgcact gttgaaaccc ggcggcgcgg cgcgcaaaac
      241 cttgcgcgca taacgcccca acaaaggaga attttttacc atgcggatac tcgttaccgg
      301 cggcgccggg tacatcggca gccacaccgt tttagaactg ctcgcggcgg gatacgacgt
      361 gcttgcggtt gacaactttg ccaactccaa acccgccgcc gtccggcgca ccggcgaact
      421 ggccgcaaag ccctttgcgt ttgagcgctg tgagctgaca aatgcggatg aagtggaagc
      481 gctgtttgcc agcaacaaaa tcgacgcagt gattcatttt gcgggtctca aagccgttgg
      541 cgagagcgtt gccaagccgc tggaatacta ccgtaacaat cttgtttcca ccctgaacct
      601 gctctttgcc atgcgcgcgc acggcgtgaa ccagttcgtg ttctcttcca gcgcaacggt
      661 ctacggcgat ccggcgagcg tccccgtccg cgaggacttc ccgctttcgg ccaccaaccc
      721 ctacgggcag accaagctca tgatcgaact catgctgcgg gacgcggtta tcgcggatcc
      781 gaatctccgc atcgcgctgc tgaggtattt caaccccgtc ggcgcgcacg tctccggcag
      841 gatcggcgag gatccgaacg ggattcccaa caacctcgta ccctatatcg cgcaggtcgc
      901 cgttggcaag ctgcccgcgc tccgcgtctt tggcgcggat tacccgaccc ccgacggcac
      961 gggagtaagg gactatatcc acgttgtcga tctcgcgcgc ggacacgttg ccgcgttgaa
     1021 aaagctgcag agcggcgcgg gcgtttatac ctataatctc ggtacgggcg taggctactc
     1081 cgtgctggac atgttgcacg cattcgaaaa ggcctgcggc aaaacgctgg cgtacgaagt
     1141 cgttgagcgc cgccccggag acattgcctc ttgttacgcc gaccccacga aggccgccga
     1201 ggagctcggc tggcgcgcgg aatacggcgt tgagcgcatg tgcgccgaca cctggcgctg
     1261 gcagtcccaa aaccccaacg gcttcgagga ttaattcagt ttctcaaagg ggctgaccgc
     1321 ccctttgtat ccccgcaagc ctcgcatggg tttccaaagg gcttgcacct tggcaggggg
     1381 tatgggggtg gaacccccga tcaatattat ttttaaagga gtattactca tgcagaaacc
     1441 cactctcgtt gtcatggccg ccggtatggg cagccgttac ggcggactca agcagatcga
     1501 cccggtcggc cgccacggcg aaattatcat ggactattcg ctcttcgacg cgatgcaggc
     1561 ggggtttgag aaggtcgtgt tcatcatcaa ccgcaagatt gagcacgact tttacgaagt
     1621 catcggccgc agagccgaaa agcatctgga agtgcattac gtcttccagc agccggaagc
     1681 gatgctcccc gccgggttcg ccgtaccgga agagcgcgtc aagccctggg gcacggcgca
     1741 cgccatcctc tgctgcaagg acgttgtgca cgagccgttt gccgccatca acgcggacga
     1801 ctactacggc aagcgcgcct tttccgtttt gttcgactac ctcaagagcg cgcgcgacgg
     1861 cgaaaagtac gacttctcca tggtcggcta tgaagcacag aacacgctca cggaaaacgg
     1921 gcacgttgcc cgcggcgtct gcgaggtcaa cgacaagggc gagctggtca acatcgttga
     1981 acggctgcgc atctacaaaa cgccggaagg ccccgcgtat acggaagacg agggcaagac
     2041 gtttgtccac ttccccgcgg acaacctcgt ttccatgaac ttcttcggct ttacgccgtc
     2101 gctgttcggc gaactggaag cgcggttccc gcagtttttg gaagcggcgc tgcgtgacaa
     2161 cccgctcaag ggcgagtttt tgattccgca ggaagtcggc ctgctgctgc agaagggcaa
     2221 ggcgagcgtg cgcgtgctga cttcgccgga tcgctggtac ggcgtgacct accgcgagga
     2281 caagccggaa gtcgagcgcg ttctttcgga aatgacgctt tccggcctct atccggatga
     2341 aaagctgatt ggataacaaa gaaagcagaa gggtttcatc ccttctgttt ttttgttttc
     2401 aggtaataaa ggcttctatc gccgtgtagc ctgaacagag tctccgggat tgttaagggc
     2461 cgcagtcctt aacccataca ttccggctgc gccggttcat ccggcgcaac ggatcgcccg
     2521 tttcgggcgg tttccctgtg ctctttgccg cccgcgagtc ccaaaggacg agaggcaaag
     2581 agcggttctt ccattaaaaa ctcgtttttg atggtgtaca aaaaaagcgg ctagtagccg
     2641 cttttttcat ccgaagccca gcgttccgag cctggcttct ttattctggg aacccattgc
     2701 gcccccaaac ttagtttgcg ttacccgcgg aaacggcagg cagaatgttg ccgccggaga
     2761 caaaggaatt gccgccggaa acgttgttgc cggaagcagc cccggtgggg ttgcaggctg
     2821 caaacagaaa catggcggaa accgccagca tcaacacgat catcgtcttt ttcataatag
     2881 taaatacctc caagattttt tattgcgttt ctaatgaact atgtgtacta tatgtgttag
     2941 tacgcatgta tgataacgca cgcaccgcaa acttgtcaaa ccaaaaaagg caatttgtgg
     3001 taaaattcat gaaaaggtca cagttatgac aatggaaccg tatcagcttc aacagctccg
     3061 catcaggcgg ttttcaacga acgtttacct cgcgctcgtt tgttttggta tgcccttaat
     3121 ctttacccgc ttctacttca acatcacgga aaccaagcac gttttctttc taacttgcag
     3181 cctcgtctac gcgctggcgc tggttctcat cgccattctg ttccccgcgg gatacggcgt
     3241 tgcaaagccg gagcgaaaac ccgttgcccc cgcaatatgg ctgctgatcg cgtttttcgg
     3301 ctgtctcgtg ctgggcggtg tgctcggcgg gcacccggag gacgcattct ttggccagaa
     3361 caaccgctat cagggcatat tgaccacgtt tacctattgc gtggtcgtgt ttgcgctgtc
     3421 gcgtcagacg ctcgacctgc gctttgcgga ggcggcgact gccgccggag cggcgctggt
     3481 cggcgcactc ggactgttga accattacgg aatcgatccg tttggtttta tgacgaagct
     3541 caccgaattc gacaggggac ggtttctctc caccatcggc aacgcggact tttacggcag
     3601 ctacttctct ctggcgtttc ctgtgacgtt cggcctgttc ctgcacgccg aacgaaaaca
     3661 cacccgcgca ctttccgtat ccgcgctcgt tcttgcctcg ttcggcgcac tggtggctgg
     3721 cagcgactcc accgcgctcg gcatgatcgc cgccgcggtc gttttcccgc tggtgctgtt
     3781 tggagacccg cgcggttttc gcaggta
//
LOCUS       DAOMGY010000064         3780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_25331_length_3780_cov_3.613960, whole genome shotgun sequence.
ACCESSION   DAOMGY010000064 DAOMGY010000000
VERSION     DAOMGY010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25331_length_3780_cov_3.613960"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="PLR57_01685"
     CDS             complement(<1..1455)
                     /locus_tag="PLR57_01685"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HQQ40195.1"
                     /translation="MEKIILYGSLLIALLAFAFAAWLYLWVKRQPCENQKIIKACGLI
                     RAGARTFLRKEYAVLAKFAGVAAVLILVFLPTPIWKGTWQNNVLMMVAYIFGTAFSAI
                     AGKIGLEVATIANGRSAEAAKKGIKPSFMAGFRGGAVMGMAVVGASLLGVTVVYLVTK
                     DPTTILGFSFGASSLALFAKAGGGIFTKTADISADLVGKVELGIPEDDPRSPAVIADN
                     VGDNVGDVAGMGADLFDSNVASMAAALVMGGALDAATGGKHVAMVFCFAALGLLASII
                     GVMTARIGKHGNPTKALNSSTYVTTGVYIALTAAATYFLQFPWRIWGACVVGLLVGVI
                     IGITTDYFTDDSKKPVHFVAHASSSGPAFTILSGISYGLLSVLPAMIGIAVSALAAYK
                     LCAPLAVNPADTTTYALFGISMAAVGMLSIVGMIVSNDAYGPIVDNARGLAEMGDLGE
                     EVLDITDQLDSAGNTVKAVTKGFAIAAAGLTVIAL"
     gene            complement(1620..1781)
                     /locus_tag="PLR57_01690"
     CDS             complement(1620..1781)
                     /locus_tag="PLR57_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40196.1"
                     /translation="MDLNAVASLPETLKIMGLGMLGIFIVTLAIVLVIALLNILSNPK
                     KQKKAKDNA"
     gene            complement(1800..2912)
                     /locus_tag="PLR57_01695"
     CDS             complement(1800..2912)
                     /locus_tag="PLR57_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HQQ40197.1"
                     /translation="MGAIFQNFSNLTWQQAVMWAIGALLIYLAIKKDMEPALLLPMGF
                     GAILVNIPLSGAITQGLEIGVLDVLFQGGIANELFPLLLFIGIGAMIDFEPLLTNPKL
                     MLFGAAAQFGIFFTLVVASLLGFPLKDAASIAIIGAADGPTSIFVANYLGSNYIGPII
                     IAAYSYMSLVPIVQPPIIKLLTSKKERRIRMEYTGSSVSKTTKILFPIIVTVIAGLFA
                     PRSVALVGFLMFGNLIRECGVLNSLSQTAQNALANLITLLLGLTVAANMQAASFLTLK
                     TLMIIGLGLVAFVFDTVGGVLFGKIINLFVKKKVNPMIGAAGISAFPMASRVIHKMGL
                     AEDKQNFLLMHAAGANVAGQIASVLAGGMLLNLIVK"
     gene            complement(3343..>3780)
                     /locus_tag="PLR57_01700"
     CDS             complement(3343..>3780)
                     /locus_tag="PLR57_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQQ40198.1"
                     /translation="QSAKVFADGEQTPIGPAIPLKEGKDAVIFAAGIMVHEAMQAAKR
                     LEQHGISVAVVNVFTIKPLDTETVTRYAELTGNVVVAENHNRIGGLYSAVCDCLAPKN
                     CAKIDVVAVNDEYGEVGPQDYLQKRFGLTSEHVESCVKQLLGK"
BASE COUNT          899 a         1054 c         1064 g          763 t
ORIGIN      
        1 cagtgcgata acggtaaggc cggcggctgc gatggcgaag cccttggtca cggccttaac
       61 ggtattgcca gcggaatcga gctggtcggt gatatcgagg acttcttcgc ccaggtcgcc
      121 catttcagca aggccgcgcg cattatccac gatggggccg tacgcatcgt tgctgacgat
      181 catgccgacg atggagagca tgccgacggc tgccatggag atgccaaaca acgcgtaggt
      241 cgtcgtatcc gccgggttga cggcaagagg cgcgcagagt ttatacgcgg ccagcgcgga
      301 aaccgcaatg ccgatcatgg ccggcagaac gctcagcagg ccgtaggaga tgccggagag
      361 aatcgtgaac gccgggccgg aagaactggc atgcgccacg aaatgaacgg gtttcttgga
      421 atcgtccgtg aaatagtccg tcgtgatgcc gatgataacg ccaacgagca gaccgacgac
      481 gcaagcgccc cagattctcc aggggaactg gaggaaataa gtcgccgccg cagtaagcgc
      541 aatgtaaacg cccgtcgtta catatgtact ggagttgagc gctttggtcg ggttgccatg
      601 tttgccgata cgggcggtca taacgccgat gatcgaagca agcaggccga gcgctgcaaa
      661 gcagaatacc atggcaacat gctttccgcc ggttgccgca tccagagcgc cgcccataac
      721 aagcgccgcc gccatggacg ctacattgga gtcgaagagg tccgcgccca taccggccac
      781 gtcgcctacg ttgtcgccaa cattatcggc gatgacggcg gggctgcggg gatcgtcttc
      841 cggaatgccg agttcaacct tgcctacaag atccgcgctg atatccgcgg tcttggtgaa
      901 gatgccgccg ccggccttgg caaacagcgc cagcgagctc gcgccaaagc tgaagcccag
      961 aatcgtggtc ggatccttgg tcacaaggta aactaccgta acgcccaaga ggctcgcacc
     1021 gacaaccgcc atacccatga cggcgccgcc gcggaatccg gccataaacg aaggcttgat
     1081 gcccttcttc gcggcttcgg cggaacggcc gttggcgatg gtggcaactt ccaggccgat
     1141 cttgcccgcg attgcggaaa acgcggtacc aaagatatac gcgaccatca ttaaaacgtt
     1201 gttctgccag gtgcctttcc aaatcggcgt cggcaggaag acaaggatca ataccgccgc
     1261 gacaccggcg aatttcgcca gaacggcata ttccttgcgc aggaaggtgc gcgcacccgc
     1321 gcgaatcagg ccgcaggcct tgatgatttt ctggttttcg cagggttgac gcttaaccca
     1381 gagatagagc catgcggcaa atgcgaaagc cagcagagcg atgagcaaag acccgtacag
     1441 aatgattttt tccataatgc tactctccct tatatgatcc gcccgcacgg gcggtattaa
     1501 agtgccggca ggtacggtga ataaacaccg tcgctgtcga aacgcttttt gtgttgaaat
     1561 gagaaggtgc gctggttgcc aacttccggc ggaaaaccgc cggaagtcag ataaaccggt
     1621 tacgcgttgt ccttagcttt tttctgtttc ttcgggttgg aaagaatgtt caacagagcg
     1681 atcacaagaa cgatcgcaag agtaacgatg aaaatgccca gcatgccgag acccatgatc
     1741 ttcagcgttt cgggaaggga tgcaactgca ttcaaatcca tattatatct ctcctctcct
     1801 tattttacaa tgaggttgag cagcatgccg cccgcgagaa cggaagcaat ctgcccggcg
     1861 acattcgcac ctgccgcatg catcagcagg aagttctgtt tgtcttccgc aaggcccatc
     1921 ttgtgaatca cgcgggaagc catcgggaat gcggaaatgc ccgcggcacc aatcatcggg
     1981 ttgaccttct tcttgacgaa caggttgata atcttgccaa agagcacgcc gccgacggta
     2041 tcaaacacga acgcaacaag accgaggccg atgatcatca gcgtcttaag cgtaaggaat
     2101 gacgcagcct gcatattggc ggcgaccgtg aggccgagca gcagcgtgat caggtttgcc
     2161 agcgcgttct gcgcggtctg ggaaagcgaa ttgagaacgc cgcattcccg aatcaggttg
     2221 ccgaacatca aaaagccaac cagcgcaacg gagcgcggag caaatagtcc ggcaatgacg
     2281 gtgacgataa tcgggaacag gatcttcgtc gttttggaaa cgctgctgcc ggtgtactcc
     2341 atgcggatcc tgcgctcctt cttgctggtg aggagcttga tgatcggagg ctgtacaatt
     2401 ggcacaagcg acatatacga ataagctgca atgatgatag gcccaatata gttggaaccg
     2461 aggtagttgg caacgaagat ggaggtcggg ccgtccgccg cgccgatgat ggcgatgctc
     2521 gccgcatcct tgagcgggaa accgagcaga gaagcgacca caagcgtaaa gaagatgcca
     2581 aactgcgccg cggcgccgaa gagcatgagc ttggggttcg tcagaagcgg ctcaaagtca
     2641 atcatcgcgc cgatgccgat gaacagcagc agcgggaaga gttcgttggc gataccgcct
     2701 tgaaagagga cgtccagcac gccgatttcc agaccctgcg taatcgcgcc ggagagagga
     2761 atgttcacga ggatcgcgcc aaagcccatt ggaagcagaa gcgcgggctc catgtccttt
     2821 ttgatcgcca ggtaaatcag cagcgcgccg atcgcccaca ttacagcctg ctgccaggtc
     2881 agatttgaga aattttgaaa gattgcgccc atattttctt cctttcacaa atgttccaaa
     2941 tttccgtctt atacatcata acatgggctt tgggaacagt aaagacatat tttctttacc
     3001 gtctctttac aattttctta gttttttcat gcagaattca cacctgcgcg tcgtaaacac
     3061 agcgtaaatc acctctccgc ccgtatttcg gaaggtttat aacggacatc ctgcaaaaca
     3121 cctccggttt gcacttcaaa aagcacctca atgtgtcgga attcgctaaa aaaccgccaa
     3181 aaagaaatgc gcccccgaaa aacgggggcg catttccaca gccaagtgcg gcgtgccaaa
     3241 cacaacacac cggcacccga tcaaacggcg ttccgagcgg gcgctcgcag gccgttcaat
     3301 ctatccactt gccgccgtgc ggcgaagtgc ggttttcaag gattatttcc cgagcagctg
     3361 ctttacgcag gattccacgt gttcgctcgt gaggccgaag cgcttctgca gatagtcctg
     3421 cgggccgact tcgccgtatt cgtcgttgac ggcgaccacg tcgatcttgg cgcagttctt
     3481 cggagcgaga cagtcgcaaa cggcgctgta caggccgccg atgcggttgt ggttctcggc
     3541 aacgactacg ttgcccgtca attcagcata ccgcgtaacg gtttccgtgt ccagcggttt
     3601 gatggtaaaa acgttgacca ccgccacgga aattccatgc tgttccagcc gctttgccgc
     3661 ctgcatggct tcgtgcacca taatgcccgc ggcaaagatg acggcgtcct ttccttcttt
     3721 gagcggaatc gcggggccga tgggcgtctg ttcgccgtcc gcgaatacct ttgcggactg
//
LOCUS       DAOMGY010000065         3769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_25447_length_3769_cov_3.862143, whole genome shotgun sequence.
ACCESSION   DAOMGY010000065 DAOMGY010000000
VERSION     DAOMGY010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25447_length_3769_cov_3.862143"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..2126
                     /locus_tag="PLR57_01705"
     CDS             261..2126
                     /locus_tag="PLR57_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ40199.1"
                     /translation="MELTNTDTLCMGCMRENKEEGTCPFCGFDSKDYTASPHHLPLKT
                     ILAGKYLIGRVLGEGGFGITYLGWDLNLGVKIAVKEYYPMGFVTREHNATSSVLPYFG
                     DKAAFFAAGRDRFVDEAKSLAKFYGLPGIVAVKDFFLENNTAYIVMEFVEGKTLKQML
                     ADAGGKLPAATVFEMMEPLMRSLSEVHGAGVIHRDISPDNIMVTKEGNVKLLDFGAAR
                     DYADSGNRSLSVMLKPGYAPEEQYRTRGVQGPWSDVYALCATIYRAITGVNPPESVER
                     LRADELEAPSALGAAIDPAREKALLKGMAVLQTDRYQSVIELYEALYSEQYAAGVPLT
                     PPRKATPVAAKKAAQQPKTAKKAVPATGKARKWSTPKWIALGVASLVLVSAVVFFAQA
                     AVTGDYDENSLIGKILANSDSAERKHTSDGDSGTNVVLKSDSQQTSPTATSAAATPAP
                     EKATPTVEPTATPTNVPEKEGSSEPTSAPKATATPKATAEVTPVPVPTSTTGAATGWS
                     TWVTSLPGGVSAPTYTVESKNQYRDVSYFYLEKTQYRTLIDTVDRQYWTDWSDDSSVL
                     YANNVVGWESRSVGSYITQYGSWVDGVKAAYTPSLSEPKDYQGVETRTVYRYKHN"
     gene            2246..2425
                     /locus_tag="PLR57_01710"
     CDS             2246..2425
                     /locus_tag="PLR57_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40200.1"
                     /translation="MMGTMLNLGTKALRALIVHPEAWVIAVPILAVTAVAYGVEQHRE
                     TVAKAQAEASARLSE"
     gene            2637..2855
                     /locus_tag="PLR57_01715"
     CDS             2637..2855
                     /locus_tag="PLR57_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40201.1"
                     /translation="MSMQTTVARSLTHIFRAGQSNPQIFVIALPVVAVAGIAYGACKL
                     HDYLEQKQADKLGEAIEAKRAALSSGSR"
     gene            2889..3038
                     /locus_tag="PLR57_01720"
     CDS             2889..3038
                     /locus_tag="PLR57_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40202.1"
                     /translation="MDTKTSLAANVLLYSGSILVDPCPPVLPLPALLLLAAGVAVKRY
                     LHPKG"
     gene            complement(3108..>3769)
                     /locus_tag="PLR57_01725"
     CDS             complement(3108..>3769)
                     /locus_tag="PLR57_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005521250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQQ40203.1"
                     /translation="SLEGTARFSFSPGAVLVLAACACWGLENNCTRKLSNKSAAQIVT
                     VKGLFSGAGSLIVALLLREAFPALPLLLAALALGFVAYGLSIFFYIRAQKELGAAKTS
                     VYYAAAPFIGVLFSLLIFRELPGWRFLPALALMLAGTYCSITDSVIRQHTHEHVHIHS
                     HAHVHGGVEHTHPHEHAHTHLHVHGAETGEQHTHAHTALEGHEHLHTDLEKADNPPEN
                     T"
BASE COUNT          909 a         1112 c         1025 g          723 t
ORIGIN      
        1 cattgcagac gacgcgtttg ccgttacgcc tcagcttacc atccacggcg cggagggttc
       61 cgcggcggaa gcgtacgccc aaaagttcgg catacgattt attgccgagc cgtaaaaaaa
      121 gattcaagaa tccacacaca acaaacggat tttgtgtata ctgtgccgag ttgggaaaat
      181 cagagcggac agatatcgtt gtttctgttc tgccgatgta agaaaactgt atgttcacgc
      241 acttttgaga aaggctgctc atggaactga cgaacacgga taccctatgc atggggtgca
      301 tgcgcgagaa caaggaagaa ggaacgtgcc cgttctgcgg gttcgacagc aaagactaca
      361 ccgcttcccc gcaccatctg ccgctcaaga ccattctggc gggtaagtac ctaatcgggc
      421 gcgtgctcgg cgaaggcggc ttcggcatta cgtaccttgg ttgggattta aacctcggcg
      481 tgaagattgc cgtcaaggaa tactacccca tgggcttcgt tacgcgagag cacaacgcca
      541 cctcttctgt actgccttat tttggagaca aagccgcgtt cttcgccgca gggcgcgacc
      601 gctttgtgga cgaagcaaag agcctcgcca agttctacgg cctgccgggc atcgtggcgg
      661 tcaaggactt cttccttgag aacaacactg cgtatatcgt aatggagttc gtcgagggta
      721 aaacgctcaa gcagatgctt gcggacgcgg gtggaaaact ccccgcggcg accgtgttcg
      781 agatgatgga gccgctcatg cgctcgcttt cggaagtgca cggcgcgggc gtcatccacc
      841 gcgacatcag cccggacaac atcatggtca caaaggaagg caacgtcaag ctgctcgact
      901 tcggcgcggc gcgggactat gccgacagcg gcaaccgcag tctctccgtt atgctcaagc
      961 ccggttacgc gccggaggaa cagtaccgca cgcgcggcgt gcagggaccc tggtcggacg
     1021 tttacgcgct ctgcgcaacc atctaccgcg caataaccgg cgtcaatccg ccggaatcgg
     1081 tggagcgtct ccgtgcggac gaactggaag cgccttccgc actcggcgcg gcaatcgatc
     1141 ccgcgcgcga aaaagcgctg ctcaaaggca tggcggttct gcagacagac cgttatcaga
     1201 gcgtgatcga gctctatgaa gcgctctatt cggagcagta cgccgcgggc gtgccgctga
     1261 ctccgccgcg aaaggcaacc cccgtcgccg cgaaaaaagc tgcacaacag ccgaaaacgg
     1321 caaagaaagc ggtacccgca acaggtaaag cccgcaagtg gagcacaccg aagtggattg
     1381 cgctgggcgt tgcgtcgctg gttctcgtca gcgcggtggt gttttttgcc caggcggcgg
     1441 taacgggaga ttacgatgaa aacagcctga ttggcaaaat tctcgccaat tcggattccg
     1501 ctgagcgcaa acatacctcg gatggagaca gcggaaccaa tgtggttctg aagagcgact
     1561 ctcagcaaac gtcgccaacc gccaccagcg cggcggcaac ccccgcgccg gaaaaagcaa
     1621 ccccgacggt ggaacctacg gcaacgccga cgaacgtccc cgagaaggaa gggtcgtccg
     1681 aaccgacttc cgcgccgaaa gcgacggcta cgccaaaagc aaccgcagaa gtaactccag
     1741 tgccagtgcc aacctcaact accggcgctg caacaggatg gtcaacttgg gtaacaagcc
     1801 tgcccggagg agtttctgca ccgacatata cggttgaaag caaaaaccag tatcgggatg
     1861 tatcctattt ttatttagag aaaacgcagt atcgaacatt gattgatacg gttgaccgtc
     1921 aatactggac agattggagt gacgactctt ccgttttgta cgccaataac gttgttggat
     1981 gggagagccg ttcagttggc agttatatca ctcaatatgg ttcctgggtg gatggtgtaa
     2041 aggccgctta taccccaagc ttatcagaac caaaagatta tcaaggcgta gaaacccgca
     2101 ccgtctaccg ctacaaacac aattaaccta cctcgccctc cggccacccg ccgcgagggc
     2161 ttttttcgca gtcgataaaa catcatcccc tctcctttcc gtaagcggta tgctgttgcc
     2221 aacaaagaaa gggaggtcaa ccaaaatgat gggaaccatg cttaatctcg gcacaaaggc
     2281 gctccgcgcg ctgatcgttc acccggaagc ttgggttatc gccgtaccga ttctggcagt
     2341 caccgccgtt gcctacggcg tggagcagca ccgcgaaacc gtcgcaaaag cgcaggctga
     2401 agcctccgcc cgcctttcgg aataacaatc tttgcagagc gccatcagcc cccgctgaac
     2461 tgcgccgcgc aaaggaagca actctctctc tcaggccgcc ccaaaagggg cggcttctct
     2521 tttttcccgc tgagcaaaaa acttattttc ccccatttga cagtctacaa attatcatca
     2581 cgaagaaaag cgcattatgc gaaaatggca ccatcaaagc aaggaggttt tcccttatga
     2641 gtatgcagac tacagtcgcc cgttcactta cgcacatttt ccgcgccggc cagagcaacc
     2701 cgcagatatt cgtcatcgcg ctgccggtag ttgcagttgc aggcattgca tacggagcct
     2761 gcaagcttca cgactatttg gagcagaaac aagccgacaa gctcggcgaa gcaatcgaag
     2821 ccaagcgtgc cgccctgtct tccggcagcc ggtaacccgc agtataatat cgctgaaagg
     2881 agccaactat ggacacaaag acctctcttg ccgcgaacgt gttgctctat tccggcagta
     2941 ttctcgtcga tccctgcccg ccggttctgc cgctccccgc gttgctgctc cttgcggcag
     3001 gagtcgcagt aaaaagatac cttcacccca agggataacc actgtaaaaa accactaaca
     3061 tacagcacaa aggccgcccg gcagggcggc cttgttgcgt atgttcgtca ggtgttttca
     3121 ggtggattgt ccgctttttc caaatccgta tgtaagtgct cgtgcccctc cagcgcggta
     3181 tgcgcgtggg tatgctgttc gcccgtttcc gcgccatgaa cgtgcagatg ggtatgcgcg
     3241 tgctcgtgcg gatgcgtgtg ttccacgccg ccgtgcacat gcgcgtgcga atggatatgc
     3301 acatgctcgt gcgtatgctg gcgaatcaca gaatccgtaa tggagcagta cgtccccgcg
     3361 agcatgagcg ccagcgcggg cagaaaacgc cagcccggca gttcgcggaa gattaaaagc
     3421 gaaaacagca cgccgatgaa cggagccgcc gcgtaataca cgctcgtctt ggcagctcca
     3481 agctcctttt gcgcgcgaat gtagaagaag atgctcagcc cgtacgccac aaaccccagc
     3541 gcgagcgccg caagcagcag cggcagagcc ggaaatgctt ccctgagcag aagcgccaca
     3601 atcagcgatc ccgcgccgga aaacagcccc ttaactgtga caatctgcgc cgcgcttttg
     3661 ttggaaagct ttctcgtgca gttgttttca agtccccagc aggcgcaggc ggcaagcacc
     3721 agcaccgcac ccggcgaaaa cgaaaaccgc gccgtaccct ccagcgaaa
//
LOCUS       DAOMGY010000066         3763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_25505_length_3763_cov_2.823355, whole genome shotgun sequence.
ACCESSION   DAOMGY010000066 DAOMGY010000000
VERSION     DAOMGY010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25505_length_3763_cov_2.823355"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2043)
                     /gene="dnaE"
                     /locus_tag="PLR57_01730"
     CDS             complement(<1..2043)
                     /gene="dnaE"
                     /locus_tag="PLR57_01730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQQ40204.1"
                     /translation="MAEFTHLHVHTQFSLLDGAARINDLIRRAKSTGQTALAITDHGV
                     MYGVVDFYKACKKEGVKPLIGMETYVAPRSMTDREGSQDREYAHLILLCKNETGYQNL
                     MFLSSEAFLRGFYYKPRIDYDLLSRHAEGLVCLSACLAGDIPQALLAGRYDDALAFAK
                     RLQGMFGEDFYIELQNHGIPEQLQILPGLRQIARDIGAKCVATNDVHYVSPDDAEAQD
                     VLLCIQTQRFVDEPDRMRMEGNQFYLKSAEEMLRALPDDAEALETTAEIVEKCNLEIE
                     FGKRRLPEFTAPDGMPNAAYLRKLTDEGLKKKLPQADDSVRERLEYELGVIEQMGFVD
                     YFLIVWDFIHFAKMNSIMVGPGRGSGAGSLVAYCLDITDVDPLKYNLLFERFLNPERI
                     SMPDFDIDFCYERRQEVIDYVGAKYGAGHVSQIITFGTMAARAVIRDVGRVLRVPYAD
                     VDRLSKMVPAELNMTLERALSISPELKANYESDEATKKIIDLSLKLEGLPRHSSTHAA
                     GVVISSVPITEVVPLQLNDSVVTTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLSFIG
                     EGGKPVPDLERLPFTDPAVYRMIAAGDTDGVFQLESGGMRQFLGQLKPDCFEDLIAGI
                     SLYRPGPMEQIPRYVKGKHDPASVVYAHPLLEPILGTTYGCMVYQEQVMEIVRALAG"
     gene            complement(2036..2959)
                     /gene="whiA"
                     /locus_tag="PLR57_01735"
     CDS             complement(2036..2959)
                     /gene="whiA"
                     /locus_tag="PLR57_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HQQ40205.1"
                     /translation="MSFSSRAKEDLAKIKIRTRGARLSQLSGLTLTCGALYVGRGRGV
                     TYATESLAVGKLILALASSLYEVESTIELSERKQRRPLTVVTLLGDNAERLLLDTGML
                     KNEADGFQLGGIAPETLLVSEEQKRAFLRGAFLGSGSCTNPYRGYHLEIVARSEELAR
                     QIAALISAGGPEAKLMTRKDRVIVYLKGEEVAGFLALVGANAPALAFENVRAERDFRN
                     YVNRTNNCDTANIGKTVNASTLQRQAIEIIEERMGLDRLPAPLYEAATLRMNHPEATL
                     QELAEMAEIGKSGMNHRLERLIRIAREIQHG"
     gene            complement(3109..>3763)
                     /locus_tag="PLR57_01740"
     CDS             complement(3109..>3763)
                     /locus_tag="PLR57_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HQQ40206.1"
                     /translation="LDAPFEILFLDARDDVLKKRYNEVRRRHPLGESGDANTGVKRER
                     VFLQPLRNRANYILDTSDIKPKDFPGMIAKVLPEFESKTFTLLFCSFGYKRGVPVDAD
                     FVLDMRFLPNPFYVEELRPLSGMDAAVQEYVFSQKFVPEFLKGIENMLTEMIPLYIEQ
                     DKHILRVCFGCTGGRHRSVAAAEEMAKRFAGKGMNIRVYHRDLGSEAAEIKERFENS"
BASE COUNT          765 a         1087 c         1098 g          813 t
ORIGIN      
        1 gcccgccagc gcgcggacga tctccatgac ctgctcctga tacaccatgc agccgtaggt
       61 tgtgcccaga atgggctcga gcagcgggtg cgcgtagacc acgctcgcag ggtcgtgctt
      121 gcccttgacg tagcgcggaa tctgctccat ggggccgggg cggtaaaggg aaatgcccgc
      181 gataagatcc tcaaagcagt cgggtttgag ctggcccagg aactgccgca tgccgccgga
      241 ttccaactgg aacaccccgt cggtatcccc ggcggcgatc atgcggtaca cggccgggtc
      301 ggtgaacgga aggcgctcca gatcgggcac cggctttccg ccctcgccga taaagctcag
      361 cgtgtcgcga attaccgtga gcgtccgtaa gcccagaaag tccatcttga gcaggccgag
      421 ctcttccagc gtggtcattg taaactgcgt ggtcacgacc gaatcgttga gctgcagcgg
      481 aacgacttcc gtaattggca cactggagat cacaacgccc gccgcgtggg tcgagctgtg
      541 gcggggaagg ccttccaact tgagcgaaag gtcgatgatc ttctttgtgg cttcgtcgct
      601 ctcataattc gccttgagtt cgggcgaaat ggagagggcg cgctccagcg tcatgttcaa
      661 ctcggcggga accatcttgg agagacggtc cacgtccgca tacggcacgc ggagaacgcg
      721 gcctacgtcg cgtatgaccg cgcgcgcggc cattgttcca aacgtgatga tctggctgac
      781 gtggcctgcg ccgtattttg cgccgacata gtctataact tcctgcctgc gctcatagca
      841 gaagtcgata tcgaagtccg gcatgctgat gcgctcgggg ttgaggaagc gctcgaacag
      901 gagattgtat ttcagcgggt caacgtcggt aatgtccaga caatacgcaa caaggctgcc
      961 cgcgccgctg ccgcggccgg ggccgaccat gatgctgttc atcttggcaa aatggatgaa
     1021 atcccaaacg atgaggaagt agtccacaaa ccccatctgc tcgatgacgc caagctcata
     1081 ttcgagccgt tcacggacgg aatcgtccgc ctgcgggagt ttctttttca acccctcgtc
     1141 ggtcagcttg cggagatacg cggcgtttgg catgccgtcc ggtgcggtga actcggggag
     1201 cctgcgctta ccgaactcga tttccagatt gcatttttca acgatttccg ccgtggtttc
     1261 cagcgcttcc gcatcgtccg gcagagcacg gagcatctcc tccgccgact tgaggtagaa
     1321 ctggttgccc tccatgcgca tgcggtcggg ctcgtctaca aaccgctggg tctggatgca
     1381 gagcaaaaca tcttgcgctt ccgcgtcgtc cggggaaaca tagtgcacgt cgttggtggc
     1441 gacgcacttt gcgccgatat cgcgcgcaat ctgccttagc cccggcagaa tttgcagctg
     1501 ttcgggaatg ccgtggttct gaagttcgat gtaaaagtct tcgccgaaca tgccctgcaa
     1561 gcgcttggca aaggcaagcg cgtcgtcgta gcgccctgca agcagcgctt gagggatatc
     1621 gcccgcaaga cacgccgaaa ggcagaccaa gccttccgcg tggcggctca gcagatcgta
     1681 atcgatgcgc ggcttatagt agaacccgcg cagaaaagcc tcgctcgaaa ggaacatcaa
     1741 gttctgatag cccgtttcgt ttttacaaag aagaatcaaa tgcgcgtact cgcggtcctg
     1801 cgagccttcg cggtcggtca tcgaacgcgg ggcgacatac gtctccatgc cgatgagggg
     1861 cttgacgcct tcctttttgc aggccttata aaagtcgacc acgccgtaca tcacgccgtg
     1921 atcggtaatc gccagcgcgg tttgccctgt ggactttgcg cggcggatca ggtcgttgat
     1981 gcgcgccgct ccgtcgagaa ggctaaattg cgtatgaaca tgcagatgcg taaactcagc
     2041 catgctgtat ctcccttgcg atgcggatga gccgttccaa ccggtggttc atgccgcttt
     2101 tgccgatttc cgccatctcc gcgagctcct gaagcgttgc ttccgggtgg ttcatccgga
     2161 gggttgctgc ttcgtataaa ggcgcgggca gacggtcgag ccccatgcgc tcctcgatga
     2221 tctcaatcgc ctgacgctgg agcgtggagg cgttgacggt tttgccgatg ttggccgtgt
     2281 cgcagttgtt cgtgcggttg acgtagttgc gaaaatcccg ttcggcgcgg acgttttcaa
     2341 aagcgagggc gggggcgttt gcgcccacaa gcgcaagaaa ccccgcgact tcttcgccct
     2401 tgagatagac gatcacgcga tccttgcgcg tcataagttt cgcttccggc ccgcctgcgg
     2461 agatcaatgc ggcaatttgc cttgccagtt cttcggaacg ggcgacgatt tccagatgat
     2521 acccgcggta ggggtttgtg caggagccgc tgccgaggaa cgcgccgcgc agaaacgcgc
     2581 gcttctgctc ctcgctgacg agcagcgttt ccggcgcaat gccgcccagt tggaatccgt
     2641 cggcttcgtt tttcagcatg ccggtatcca gaagcagtcg ctccgcgttg tcgccaagca
     2701 gcgtgaccac cgtgagcgga cggcgttgtt tgcgctcgct gagttcgatg gtggactcca
     2761 cctcgtaaag cgagcttgcc agcgcgagaa tcagcttgcc tacggcaagc gattcggttg
     2821 cgtacgtcac gccccggcca cggccgacat acagcgcgcc gcaggtcagc gtcagcccgg
     2881 agagctggct caggcgcgcg ccgcgcgtac ggattttgat tttggcaagg tcttccttgg
     2941 cccggctgga aaaggacatc tgtaaaacac tccataactc tggttcgaat gaaaccttct
     3001 tctattataa taagaaagct ggaacgaatg atagcaaaat aagaaaaatc cttcttaccg
     3061 aatgaaacat tattacggtt gactatataa aagaaaaact cctgttattc aggagttttc
     3121 aaaccgttcc ttgatttccg ccgcttcgga gcctaaatcg cggtgataca cccgtatgtt
     3181 catgcccttt ccggcgaagc gtttggccat ttcttccgcc gcggcaaccg agcggtggcg
     3241 cccgcccgta cagccaaagc agacacggag gatgtgcttg tcctgctcga tatacagcgg
     3301 aatcatctcc gtaagcatgt tctcgatgcc ttttaaaaac tccggcacga acttctgcga
     3361 aaaaacatac tcctgcaccg cggcgtccat gccggaaagc gggcgcagct cttccacata
     3421 aaacggattc ggcagaaagc gcatgtcgag cacgaaatcc gcgtctaccg gcacgccgcg
     3481 tttgtaaccg aaactgcaga ataagagcgt aaacgttttg ctttcaaatt ccggcagcac
     3541 ttttgctatc attccgggga aatccttggg cttgatgtcg ctggtgtcga gaatgtagtt
     3601 ggcgcggttt cgcagcggct gcagaaacac gcgttcgcgc ttgacgccgg tgttcgcgtc
     3661 ccccgactcg ccaaggggat ggcggcggcg cacttcgtta tagcgttttt tcagcacgtc
     3721 gtcccgcgcg tcgagaaaca gaatttcaaa cggcgcgtcg agg
//
LOCUS       DAOMGY010000067         3728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_25836_length_3728_cov_3.156276, whole genome shotgun sequence.
ACCESSION   DAOMGY010000067 DAOMGY010000000
VERSION     DAOMGY010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25836_length_3728_cov_3.156276"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLR57_01745"
     CDS             complement(<1..561)
                     /locus_tag="PLR57_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40207.1"
                     /translation="MAKDWIGTNGKCKYCGLDEAHCVCAKPENDSCDITCSDESCSCG
                     HAHESEEHEHGGEGARQFLILGVAAAMLIASHFIGDGFWQTALRIVSYLAAGYEVLLR
                     AGKGLFKKRFFDENVLMSIATIGAIALGDYSEAAAVMIFYGVGESLQELAVRSSRKRI
                     SDAVELHPDKARLVSGDEERMVKPEEV"
     gene            complement(554..937)
                     /locus_tag="PLR57_01750"
     CDS             complement(554..937)
                     /locus_tag="PLR57_01750"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQQ40208.1"
                     /translation="MNVISGTPICGETHIDKQHEDEVRAHLPSEDEAADAAAVFTHLS
                     DTTRVKLLSMLAVSDMCVCEMADLLGMSQPAVSHHLRLLRQCDVIRFRKRGQRTVYYL
                     SDNEIGDTVKRLLYAVKNGKGQNNG"
     gene            complement(1065..1736)
                     /locus_tag="PLR57_01755"
     CDS             complement(1065..1736)
                     /locus_tag="PLR57_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQQ40209.1"
                     /translation="MDYKAIADQMGPRVGATQEWIFHVLREGIISGLLPGGMQLKQDE
                     ISAALNVSHIPVREALRRLEAQGLVRIHPNRGASVTQLTRDMLLDMMEVRATLSVMML
                     KNNAPLLTQEDFDALEAVVKQQRETPVDDIVRSEELNYKFHEILTSRADNSMADLMLE
                     LIHANIDRYLRANFYGSLETREVSIGQHEAILAACRKGDFEAACSLLRDHILNAKEFI
                     PASLK"
     gene            complement(2005..2340)
                     /locus_tag="PLR57_01760"
     CDS             complement(2005..2340)
                     /locus_tag="PLR57_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQQ40210.1"
                     /translation="MDIGASIKRLRKSHGETQQQLGEAVGVVQQSVAAWESNRCMPDI
                     ASLVAIATHYGTSTDSILGLTEEPSYLLDTEGMQSYRNDVALADRLPMEIREGVLALI
                     RLERAKYGR"
     gene            3219..>3728
                     /locus_tag="PLR57_01765"
     CDS             3219..>3728
                     /locus_tag="PLR57_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HQQ40211.1"
                     /translation="MGIRLAVVGAGSTYCPELMQGLIKRRDILPLGELRLTDIDESRL
                     NIVGDFCERVLLENDMEPRILRSVDLARAVEGADYVVTQIRAGKLEARVRDEKIPLEY
                     GFIGQETTGIGGFTNALRTIPQMELIAEAVSKYAPDSWLINFSNPSGIVTEFLLNYTP
                     VKAIGLCNLP"
BASE COUNT          833 a         1011 c          931 g          953 t
ORIGIN      
        1 gacttcctcc ggcttgacca tccgctcttc atccccgctg accagacgcg ccttgtcggg
       61 atgcagttcc accgcgtccg aaatccgctt gcggctggaa cgcacggcca gctcctgcaa
      121 gctctcgcct acgccgtaaa aaatcatgac cgccgccgct tccgaatagt cgcccagcgc
      181 gattgcgccg atggtggcaa tgctcatgag cacgttttcg tcgaagaacc gctttttaaa
      241 cagtcctttg cccgcgcgca gcagaacttc gtatcccgcc gcaagatagg aaacaatacg
      301 cagcgcagtc tgccagaaac cgtcgccaat aaaatggctt gcaatcagca ttgccgcggc
      361 aacgcctaat atcaaaaact gacgggcgcc ttcgccgccg tgttcatgct cttcgctttc
      421 atgcgcgtgt ccgcaggagc agctttcgtc gctgcaggtg atatcgcagg agtcgttttc
      481 cggtttcgcg caaacgcagt gcgcctcgtc cagtccgcag tacttgcatt tcccgttggt
      541 tccaatccag tccttagcca ttgttctgac cctttccgtt ctttacagca tataaaagcc
      601 gcttgaccgt atccccgatt tcgttgtcgc tgaggtaata caccgtcctc tgcccccgct
      661 tgcggaaacg aatcacgtcg cactgccgca ataagcgcag atggtgcgaa accgcgggtt
      721 ggctcatgcc caataagtcc gccatttcgc agacgcacat atcggatacc gcgagcatgg
      781 aaagcagttt gacgcgcgtg gtgtccgaaa ggtgggtaaa aaccgccgcg gcgtccgccg
      841 cttcgtcctc gctcggcagg tgcgcgcgca cctcgtcctc atgctgcttg tcgatatgcg
      901 tttcgccgca gatcggtgtt ccggagatta cgttcataag cgcctcttta tataagtgat
      961 tgcttatata ttagcatttt actaggcttt gtcaagacac aaatgcacag aaaaaataaa
     1021 aacaggctcc tctttcgaaa agcctgtcgg ttcacgatct gccgttattt cagcgacgcg
     1081 gggatgaatt cctttgcgtt gaggatgtgg tcacgcagca gactgcaggc ggcttcaaaa
     1141 tcgccttttc tgcaggccgc gaggattgcc tcgtgctggc caatggagac ttcccgcgtt
     1201 tccagcgagc cgtagaagtt ggcgcgcagg taacggtcga tgtttgcgtg aatcagctcg
     1261 agcatcaggt cggccatcga gttgtccgcg cgggatgtca gaatttcgtg gaacttgtag
     1321 ttgagctctt cggaacgtac aatgtcatcc acaggcgttt cgcgctgttg tttgacaacc
     1381 gcttccagcg cgtcgaaatc ttcctgtgtc agcagcggcg cgttgttttt gagcatcatc
     1441 accgaaagcg ttgcgcggac ttccatcata tccagcagca tgtcgcgcgt cagctgggta
     1501 acggacgctc ctctgttggg gtggatgcga accagtccct gcgcttccag cctgcgcagc
     1561 gcctcgcgca ccgggatatg gctgacgttc agcgcggcgg aaatttcatc ctgcttgagc
     1621 tgcatgcccc cgggcaaaag cccgctgata ataccttccc gcagaacgtg aaaaatccat
     1681 tcctgcgtgg cgccgacgcg cggccccatc tgatcggcaa tggctttata gtccatattt
     1741 cacctcataa aaaatcgcga cttgaaaata acggtgatgt gccgtccaga gagtattata
     1801 taggatgttt tcgccccgcg caaccccccg cccccgcggg tggatacgcg gctgccgctt
     1861 gtccgtcatg gttaacttta acaagcccac gcagggtacc gacgaagaac acaggtttct
     1921 actcgttcaa gctaggtggt ttttctgtag gttttgtttg gtgcccgtta cgctctttac
     1981 agcgcgataa caaaaggtgg tttgttatct gccgtacttt gcgcgctcca gacgaatcag
     2041 cgcgagaacg ccttcacgaa tttccatggg cagacgatcc gccagcgcca cgtcgttgcg
     2101 gtaggactgc atgccttccg tatcgagcag atagctgggt tcctcggtca ggccaagaat
     2161 gctgtccgtg gacgtgccgt agtgggtggc gattgcaacc agcgacgcga tgtccggcat
     2221 gcagcggttg gactcccaag ccgcgaccga ctgctgaact acgccaaccg cttcgccaag
     2281 ctgctgctgc gtttccccgt gtgattttct gagtctcttg atgctcgctc caatgtccat
     2341 tgtctctctc tccttcgaat caattccaac agcgtacgtt cgtgattcaa caggcaaacg
     2401 ccgcttggtt ccagtgtttg gcaggcgctg atttttacat attaatgcag tcagcacact
     2461 atttctaaca ctgtatgtca tattgtacta cacccgtgga cagaaagaaa gagccagatt
     2521 gaaaaaaata ctattttttg cgatattttc tacagaaata tctaaatgca cattttcccg
     2581 ggatcaaaac agcgttttat tatttaatca tcgttcaatt cattaaacgt ctattatttt
     2641 ctgtcggtgg ttgtatatat gagtgcagcg tcaaaaataa tcgaaaattg atgtaaagca
     2701 tgaaagtttt ctaatcatta ttcgcgatcg aggtgaacgt ttttcgtcaa aaacgcgcaa
     2761 ggttaatgtc gtccaactgt acagccattg tttttcacag aatgcttgac cgttttaact
     2821 tgtacaacac aatatgtaag attcacgtaa caatttgcaa aaaaaacgaa cgtatctcac
     2881 tctgttatta acagtgatgc attttgcaca ctgaacatcc ccgttcgtaa acgattacga
     2941 aaatgttgtt gtatcaacca gtttatcgat ttcatttttc aaggccgctt tgttacaatt
     3001 ttggtgattt tgcgttcgtt ttttacgtat tttttacttt gcgtaataca aagtatgata
     3061 tactttccat aaacgctttg ttcatcaaag catcctctgt cggcgcgtgg tatccagccg
     3121 ttcagaattg cgctttacga gtcaaacgac ccgcatttcg atggtttttc attaacacaa
     3181 agaactacta aatagaaaat gaatcaggag gtgcggtcat gggaattcgg ctggcggttg
     3241 tcggcgcggg cagcacctat tgcccggaac tgatgcaggg gttgatcaag cgcagggata
     3301 tcctgccctt gggcgaactg cggcttacgg atattgacga atcccggctc aatattgtcg
     3361 gggatttctg cgagcgcgtc ctgctggaaa acgacatgga gccgcgcatt ctgcgcagtg
     3421 tcgatctggc gcgcgccgtg gaaggcgcgg actacgtcgt cacccagatt cgcgcgggca
     3481 agctggaagc gcgcgtgcgg gacgaaaaga ttccgctcga atatggtttc atcgggcagg
     3541 agacgaccgg catcggcgga tttaccaacg cgctgcgcac catcccgcaa atggagctta
     3601 ttgcggaagc ggtttcgaag tacgcgccgg attcctggct catcaacttt tccaacccct
     3661 ccggcatcgt aacggaattc ctgctcaact atacgcccgt caaggcaatc gggctttgca
     3721 acctgccc
//
LOCUS       DAOMGY010000068         3718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_25929_length_3718_cov_2.896260, whole genome shotgun sequence.
ACCESSION   DAOMGY010000068 DAOMGY010000000
VERSION     DAOMGY010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25929_length_3718_cov_2.896260"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLR57_01770"
     CDS             complement(<1..495)
                     /locus_tag="PLR57_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009753838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HQQ40212.1"
                     /translation="MRLRVNDMEQNEQREVRRIDARGTDYQALNACIRQATADVEIVG
                     ALGQRYLGDAMDSGTLTIRGTAGNALGAYLNGGRIVVYGNAQDAAGDTMNKGEIIIHG
                     SVGDTVGYSMRGGCIYVRDSAGYRAGVHMKAYGETSPVLVVGGRCGSFLGEYQAGGTI
                     VVLGL"
     gene            complement(606..2111)
                     /locus_tag="PLR57_01775"
     CDS             complement(606..2111)
                     /locus_tag="PLR57_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HQQ40213.1"
                     /translation="MPVSELYPEYELIRNERRCTNCRICERQCANGVHKLDARSGRMV
                     ADESKCVNCQRCVAYCPTHALKIVKTQNACRENHNWSCDAIKEITLQAGSGGVLLSSM
                     GNPREYPVYWDRLLVNASQVTNPSIDPLREPMETRVFLGKKPTSIARTQDGVLVSDLP
                     PQIELKLPILFSAMSYGSVSRNVHEALARAATELGTCYNTGEGGLHESLYPFVKNTIV
                     QVASGRFGVHKEYLNAGAAIEIKMGQGAKPGIGGHLPGTKIVGDVSKTRMIPEGSDAI
                     SPAPHHDIYSIEDLQQLVYSLKEASGYQKPIIVKVAAVHNIAAIASGIARSGADIIAI
                     DGFRGGTGAAPTRIRDNVGIPIELALASVDARLRDEGIRNSVSLVIGGSIRSSADAVK
                     AIALGADAVYIGTAALIALGCHLCRSCHKGACNWGIATQRPDLVDRLDPEEGAQRLIN
                     LVTAWNREIREFLGGMGINSIEALCGNRAMLRGIGLNEAELRVLGVKHAGE"
     gene            complement(2102..3205)
                     /locus_tag="PLR57_01780"
     CDS             complement(2102..3205)
                     /locus_tag="PLR57_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40214.1"
                     /translation="MALREGEVRIPSGCAIAGVFSKEGNLIPGSLVTHAMTSMHERSN
                     GLGGGFAGYGIYPQNKEFYALHVFYTDSDARAACERYLDQVFEVVNLSKIPTRKTKKI
                     TDEPLIWRYFVAPQPNRLRDSQLDEKEYVARAVIHVNSTFEGSFVFSSGKNMGVFKGV
                     GYPEDIGDFYRLDEYEGYCWTAHGRYPTNTPGWWGGAHPFALLDYTIVHNGEISSYDA
                     NRRFIEMFGYRCTLQTDTEVMTYIADYLLRKVKLTLSEFASVIAAPFWSTIAQKDEAE
                     QKRLTYLRNVFGSLLVTGPFSILLGFEGGMLALNDRLKLRSMVVGERGDRVYVASEEC
                     AIRYMEPTLDRLYAPRGGEPVIVTLNGGVEQCL"
BASE COUNT          735 a         1080 c         1115 g          788 t
ORIGIN      
        1 gaggccgagc acgacgatgg ttccgcccgc ctggtattcg ccaagaaagc tgccgcaccg
       61 cccgccgacc accaatacgg gggaagtttc gccgtacgcc ttcatatgca cgcccgcgcg
      121 gtagcccgcg ctgtcgcgca catagatgca cccgccgcgc atgctgtagc caacggtatc
      181 gccaaccgaa ccgtggatga tgatttcacc cttgttcatc gtgtccccag ctgcgtcctg
      241 cgcgttgccg taaacgacga tgcgcccgcc gttgaggtaa gcgccgaggg cgttgcccgc
      301 cgtgccgcgg atggtgagcg tgccgctgtc catcgcgtcg cccagataac gctggccgag
      361 cgcgccgaca atttccacgt ccgccgtggc ttggcgaatg caggcgttga gcgcttgata
      421 atccgtgccc cgcgcgtcga tacgccgcac ttcgcgttgt tcgttctgct ccatatcgtt
      481 cacccttaat ctcaaattgg taacgccaaa ggggtgttca cccctttgga aaccccatat
      541 caggtggggt tcttagggga gaattcccct aaggcagggg tttggggcgg agccccaaga
      601 agtccttact ctcctgcgtg cttgacgccg agcaccctca gctccgcctc gttgaggccg
      661 atgccgcgca gcatggcgcg gttgccgcag agcgcttcga tggagttgat gcccattccg
      721 ccgaggaact cccgaatctc gcggttccag gcggtcacga ggttgataag ccgctgcgcg
      781 ccttcctccg ggtcgagccg gtcgaccagg tccgggcgct gggtggcgat gccccagttg
      841 caggcgcctt tgtggcagct gcggcagaga tggcagccga gcgcgatgag cgctgcggtg
      901 ccgatataga ccgcgtccgc accgagggcg atagccttga ccgcgtccgc actggagcgg
      961 atgctgccgc cgataacgag ggaaacgctg ttgcggatgc cttcgtcgcg gaggcgcgcg
     1021 tccacggaag cgagcgcaag ttcgatggga atacctacgt tgtcgcgaat gcgggtgggc
     1081 gctgcgcccg tgccgccgcg gaagccgtcg attgcgatga tgtccgcgcc gctgcgggcg
     1141 atgccgctgg cgatggcggc aatgttgtgc accgccgcaa cctttacgat aatcggcttt
     1201 tggtagccgc tcgcttcctt taaagaatag acgagctgct gcagatcctc aatggaatag
     1261 atatcgtggt gcggcgcggg ggaaatcgcg tccgaccctt ccggaatcat gcgcgttttc
     1321 gacacgtcgc caacgatctt ggtgccgggc aaatgtccgc cgatgccggg ctttgcgccc
     1381 tgacccatct tgatttcaat ggccgcgccc gcgttgagat attccttgtg cacgccgaag
     1441 cgcccggagg ccacctgaac gatggtgttt ttcacgaagg ggtagagcga ctcgtgcaaa
     1501 ccgccctcgc cggtgttgta gcaggtgcca agctccgttg ctgcgcgggc gagcgcttcg
     1561 tgcacattcc ggctcaccga gccgtagctc atggcggaaa acagaatcgg caacttaagc
     1621 tcaatttgcg ggggcaggtc ggaaaccagc acgccgtcct gcgtgcgcgc aatggacgtc
     1681 ggctttttgc cgagaaaaac gcgcgtctcc atcggttcac gaagcgggtc gatggaaggg
     1741 ttggtcacct gagaagcgtt tacgagtagc ctgtcccaat agaccgggta ttcgcgcggg
     1801 ttgcccatgg aggagagcag cacgccgccg ctgcccgcct gaagcgtgat ctccttaatg
     1861 gcgtcgcagc tccagttgtg attttcgcgg caggcgtttt gcgtttttac aattttcagc
     1921 gcgtgcgtcg ggcagtaagc cacacagcgc tggcagttta cgcacttgga ttcgtccgca
     1981 accatgcggc cggagcgcgc gtcgagctta tggacgccgt tggcgcactg gcgttcacag
     2041 atgcggcagt tggtgcagcg ccgttcgttg cggatcagtt catattccgg gtagagttcg
     2101 cttacaggca ctgctcgaca cctccattca gggttacgat aaccggttcg ccgccgcgcg
     2161 gggcgtaaag ccggtcgagc gtcggttcca tatagcggat ggcgcattct tcgctggcaa
     2221 catacacccg atccccgcgt tcgccaacca ccatggagcg aagcttcagc cggtcgttga
     2281 gcgcgagcat gccgccttca aaccccagca ggatggagaa cggccccgtt accagcaggc
     2341 tgccgaatac gttgcgcagg tacgtcaggc gcttttgctc cgcttcgtcc ttctgcgcga
     2401 tggtgctcca aaagggagcg gcgatgacgc tggcgaattc gctgagggtc agcttgacct
     2461 ttctaagcag atagtccgca atataggtca tgacttcggt atccgtctgc aaggtgcagc
     2521 gatagccgaa catttcaata aagcggcgat tcgcgtcgta actcgaaatc tcgccgttgt
     2581 gaacgatggt gtaatccaga agcgcaaacg ggtgcgcgcc gccccaccag ccgggggtgt
     2641 tggtcgggta ccgcccgtgc gcggtccagc agtagccctc gtattcgtcg agacggtaaa
     2701 agtcgccaat gtcctccggg tagcccacgc ctttgaacac gcccatgttc ttgccgctcg
     2761 aaaacacaaa gctgccttcg aacgtggagt tgacgtggat caccgcgcgc gcgacgtatt
     2821 ccttttcgtc aagctggctg tcgcgcaggc ggttcggctg cggggcgaca aaatagcgcc
     2881 agatcagcgg ctcgtccgtg attttctttg tcttgcgcgt gggaattttt gagaggttga
     2941 ccacctcgaa cacctgatcc agataccgct cacaggcagc gcgcgcgtcc gaatcggtat
     3001 aaaaaacgtg cagcgcgtaa aactccttgt tttgcggata gatgccataa ccggcaaatc
     3061 cgcccccaag gccgttggag cgctcgtgca tgctggtcat ggcgtgcgtg acgagacttc
     3121 ccggaatgag gttgccctcc ttcgaaaaga ctcccgcgat cgcacagccg gacggaatgc
     3181 ggacttcacc ttcccgcagc gccatagtcg tttctcctta cgcgttagat tcctgtttct
     3241 gcgcccgttt tggggataaa taccggtttt ttgccggatt ttccctgctt gggcgcaaca
     3301 ggcgtccttt tttgggtgat atacagcggg ccaaccgccc gtttgaacgc aaaaagcgtc
     3361 cacacggcgg acgatttgcc cgcgtgatga acgccgttgt tccacggcag tatacataat
     3421 ataggatatc ctgtcaagca taaaatgaat gatatatact tataaactgc aaaaaaccaa
     3481 aaacagaata tttttggtga gacaaaaaag aattccgtgc aggaatttga attaatctgc
     3541 aggatatcct gcaaaaagaa agggttgaca agggttatac cccgctgtat aatacccgca
     3601 tattatttca aaacggcaaa ggcgccgcgg ttggaaccgt ggtgtcgttg cctcttttta
     3661 taagaggcag gaggagaaaa taatcatggg tatggtaacg gaaacatttg gctgcaag
//
LOCUS       DAOMGY010000069         3704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_26061_length_3704_cov_2.723760, whole genome shotgun sequence.
ACCESSION   DAOMGY010000069 DAOMGY010000000
VERSION     DAOMGY010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26061_length_3704_cov_2.723760"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLR57_01785"
     CDS             <1..384
                     /locus_tag="PLR57_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40215.1"
                     /translation="LTLPLGWIAEESENSLACFHPKGEGAITISLHAAETGGETLTQY
                     LILLAKRYIKQNEICLTSRLIPDDSNPNCVFIFAEGEQPDGWKTAFRFVSNGQQVAMF
                     TYLCQSRTREWNKAVKIAANAVFIG"
     gene            495..1274
                     /locus_tag="PLR57_01790"
     CDS             495..1274
                     /locus_tag="PLR57_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HQQ40216.1"
                     /translation="MLRGERCQTYEGFFSECAAAFQFPNYFGNNWNAWDECINDLDWL
                     EFTSLAIVIDCFELLFSKEGHLARDRYLLEQSFDEWTRYWQEEKGVSCFVTAFSEEKL
                     VLPRYVLPERLNGHFRITDVKTKADTVLTGYLECCSDRAFEVFYDAKLKRSWIGEYSL
                     NATARGFAFLARCARCGGEIRLLNCRREEELTSAIPHQLFCPKCGKNEFALKVSLEYP
                     SDAAEQEETNERGEAFGWIWVAASCCACGKELKHLVVLEND"
     gene            complement(1351..2982)
                     /locus_tag="PLR57_01795"
     CDS             complement(1351..2982)
                     /locus_tag="PLR57_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40217.1"
                     /translation="MKRFNRSSTDHLLTLGMALYADQKTMERRVRGVFARKKSTKPAK
                     LLTLALCLALAAGCFTTACQPGAQPASNGNAAVSGGNAALVSGGNASSGNASTGGISA
                     AGMTPEEAKAQTMRWHQDELTQARTFVAPPVRDIYRDPLGDWRADEDKNAAPAEAASA
                     AEAFLAVANQVFNTSYTAADMTATYYTDASGFRSDVYRFDSKDGVLSGAVDAKTFAFI
                     AANCGVEPADTAHQSLLDAGEKLDTDFGDELLDTTDAAVRLAGIFGGDASAAVKQGSS
                     YGDNKLAGWNMQVTVTIPLKDGKFCRAMVFGDENLTVSSIGVYPDSNCADEDVYWRAD
                     LIWTEGVITLAAPQDFRKGEPGADDMPVEQAYDLYYKLVAAAGTPDGRAAEPPKEPNA
                     TFYVDYSGARDNYWHIEGKYARFDLTSQTGHMLNLESNSMLGFSMDLMEIDYDHMGGQ
                     EYIDATRALFTALFGEGSVKDVMVNAVYDYHYCTVDPVMVDGTAYEIMFQDGLIVEAT
                     FFSSQEKSGFGTDPNWAADWIYKNNETGETFHMEW"
     gene            complement(2954..>3704)
                     /locus_tag="PLR57_01800"
     CDS             complement(2954..>3704)
                     /locus_tag="PLR57_01800"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HQQ40218.1"
                     /translation="LPQSQTPTVALQPVGTTAEPIVDAAAPSTEPEPAADAPAGMQTK
                     TDSPRVSAQPLTLWQWLLIAWAAGFALVLTAHLALSLQLNRRIKQLGCPPGDRTQTLY
                     REVKQGMRIRADVPIVLMADIKSPALTVQFRPKLLLPDRLLYAADREHMAFAMAHELM
                     HYRRRDYLVCLLISLLRAVYWFNPIVWIMPRILRLDMESACDAMVVKSMSREQKLSYV
                     NLLLALAEETSAKEITEEGDFTHETIQPVVN"
BASE COUNT          735 a         1067 c         1104 g          798 t
ORIGIN      
        1 ctgaccctgc cgctcggctg gattgcggag gaaagcgaga acagcctcgc ctgctttcac
       61 ccgaagggcg aaggcgcgat aacgatttcc ctgcacgcgg cggaaacggg cggcgaaacg
      121 ctgacccaat acctgattct gctggcgaag cgctacatca agcagaatga aatctgcctg
      181 acgagcaggc tgattccgga cgattcgaac ccgaactgcg ttttcatttt tgcggaaggc
      241 gaacagccgg acggctggaa aacggcgttc cggttcgtct ccaacggaca gcaggttgcg
      301 atgttcacct atctttgcca gtcccgtacg cgagagtgga acaaagccgt taagatagca
      361 gcgaacgcgg ttttcatcgg ctgaagggca gaaagggagc ttccattggt ccgaattgga
      421 tcagacaggg aacctgtgaa gacgcttctg cggcgttctc cgaacacggt cagacgcccg
      481 ggcgctatgt tgccttgctg cgcggcgagc gctgccaaac atacgaaggc ttcttttcgg
      541 aatgcgcggc tgcgtttcag tttccaaact actttggaaa taactggaac gcctgggacg
      601 aatgcatcaa cgatctcgac tggctggagt tcacctcgct tgcgatcgtc atcgactgtt
      661 ttgagctgct tttcagcaaa gaagggcacc ttgcgcgcga ccgctatttg ctggagcagt
      721 cctttgacga atggacgcgc tattggcagg aggaaaaagg tgtttcctgc tttgtcaccg
      781 ctttttcgga ggagaaactg gttctgccga gatacgtcct gcccgaacgg ctcaacggcc
      841 attttcgcat caccgacgtc aaaactaagg cggatacggt gctgaccggt tatctggagt
      901 gctgcagcga ccgcgcgttc gaggtgtttt acgacgcgaa gctcaagcgg agctggatcg
      961 gcgaatattc gctcaatgct acggcgcgcg ggtttgcgtt tttagcgcgc tgcgcccgtt
     1021 gcggcggcga aattcgcctg ctgaactgcc gcagagaaga agaactgacc tcggcgattc
     1081 cgcatcaact attctgcccc aagtgcggca agaacgagtt tgcgctcaag gtgtcgctgg
     1141 agtacccgtc cgacgccgcc gagcaagagg aaacaaacga gcgcggcgag gcgttcggct
     1201 ggatctgggt tgcagcttcc tgctgcgcct gcggaaaaga attgaaacat ctggtcgtgc
     1261 ttgagaacga ctgattaggg ttttatttga atgtaagaac atgaaaaagc cgcacgggat
     1321 ttcccgtacg gctttctagc atcttctgat ttaccactcc atatggaatg tctcgcccgt
     1381 ttcgttgttc ttgtaaatcc agtcggccgc ccagttcggg tcagtgccaa agccgctctt
     1441 ttcctgcgac gagaagaacg tagcctccac gatcaatccg tcttggaaca taatttcgta
     1501 ggcggttccg tccaccatga cgggatcgac cgtgcagtag tggtaatcgt ataccgcgtt
     1561 gaccatcacg tcttttacgc tgccttcgcc gaacagcgcg gtgaagagcg cgcgcgttgc
     1621 gtcgatatac tcctgcccgc ccatgtggtc gtagtcgatc tccataagat ccatggagaa
     1681 accgagcata ctgttggatt ccagattcag catgtgtccg gtctggctgg tcaaatcgaa
     1741 tcttgcgtat ttaccctcaa tatgccagta gttatcccgt gcgccggaat agtccacgta
     1801 aaacgtcgcg ttcggctctt tgggcggttc ggcggctctg ccgtccggcg tacccgccgc
     1861 ggcaacgagt ttgtagtaga ggtcgtaagc ctgttcaacg ggcatatcgt ctgcgccggg
     1921 ttcacccttg cggaagtcct ggggagctgc cagcgtgata acgccttccg tccagatgag
     1981 gtcggcgcgc cagtaaacgt cctcgtccgc gcagttggaa tccgggtaaa cgccgatgct
     2041 gctgacggtc agattctcgt ctccaaacac catcgcacgg cagaatttgc cgtccttgag
     2101 cggaatcgtt accgtcacct gcatattcca tccggccagc ttgttgtcgc cataggaaga
     2161 accctgtttg accgcggcgc tcgcgtcccc gccgaaaata ccggcgagcc gtacggcggc
     2221 gtcggtcgta tcgagcagtt cgtcgccaaa gtccgtatcc agcttttctc ctgcgtcgag
     2281 caggctttga tgcgccgtat cagcgggttc caccccgcag ttggcggcaa tgaacgcaaa
     2341 ggtcttcgcg tccaccgcac cggagagcac gccgtccttg gaatcaaagc ggtagacgtc
     2401 actgcggaag ccggacgcgt ccgtatagta cgtggcagtc atgtccgccg cggtgtaact
     2461 ggtattgaac acctggtttg caacggcgag gaacgcttcc gccgcactcg ccgcttcggc
     2521 gggcgccgca ttcttgtcct catccgcgcg ccagtcaccg agcggatcgc ggtagatatc
     2581 ccgcaccggc ggagctacaa acgtgcgcgc ctgcgtaagt tcgtcctggt gccagcgcat
     2641 ggtttgcgct ttcgcttctt cgggcgtcat gccagcggcg gatatgccgc cagtggatgc
     2701 gttgccggag gaagcgttgc cgccggagac cagcgcggcg ttcccgccgg aaaccgctgc
     2761 gtttccattg cttgcgggct gcgcgcccgg ctgacaggcg gtggtaaaac agcccgccgc
     2821 aagagccaga cagagcgcaa gcgtcagcag tttcgcgggt ttcgtgctct ttttgcgggc
     2881 gaacacgccg cgtacgcgcc gttccatcgt tttttgatcg gcgtacagcg ccatgccgag
     2941 ggtcagtaaa tggtcagttg acgaccggtt gaatcgtttc atgggtaaaa tctccttctt
     3001 cggtgatctc tttggcggat gtttcctccg ccagcgccaa caagagattg acataactca
     3061 gcttttgttc tctgctcatc gattttacga ccatcgcgtc gcaggcggat tccatatcca
     3121 gccgcagaat gcgcggcata atccatacga tggggttgaa ccagtaaacg gcgcgcagaa
     3181 gcgaaatcag caggcagacg aggtagtccc gccgccggta gtgcatcagc tcatgcgcca
     3241 tggcaaacgc catatgctcg cgatccgccg cgtagagcag gcgatccggc agcagaagtt
     3301 tgggtcgaaa ctgcacggtc agcgcggggc ttttgatatc cgccatcagc acaatgggaa
     3361 cgtccgcgcg aatacgcatg ccctgcttga cctcccggta gagcgtctgc gtcctatcgc
     3421 cgggcggaca gccgagctgt tttatccggc ggttgagttg aagcgaaagc gccagatgag
     3481 ccgtcagtac gagcgcgaat cccgccgccc aggcaatcag cagccactgc cagagtgtga
     3541 gcggctgtgc cgaaacgcgc ggagagtcag ttttcgtttg catgcccgcg ggcgcgtctg
     3601 ccgccggttc cggctccgtg gatggagcgg ccgcgtccac aatcggctcc gcggtcgtgc
     3661 ctacaggttg cagggcgaca gtcggggtct ggctctgcgg cagg
//
LOCUS       DAOMGY010000070         3681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_26298_length_3681_cov_3.046608, whole genome shotgun sequence.
ACCESSION   DAOMGY010000070 DAOMGY010000000
VERSION     DAOMGY010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26298_length_3681_cov_3.046608"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2702)
                     /gene="pepF"
                     /locus_tag="PLR57_01805"
     CDS             complement(<1..2702)
                     /gene="pepF"
                     /locus_tag="PLR57_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HQQ40219.1"
                     /translation="MNRVQRQDETEITTRDIARSARKLLRARFFSLLKRFFLHAGLAL
                     MVLVSAGAFLAAAALQPELLGVVRSGFRAEALLIFCGIPAILLTGLYAAYLHPRAVVA
                     VYETYAEAQFVAPRGGGSLTSANRSYVRVKGAYWAAALVWLCGGLAAAGFCWPLTLAA
                     EKIPRFGSFCSSAVLTLGLSLFWLLRVPLGLSLAAAARENIGGFRALRRGFLLHRRVR
                     QTASAVYMPWLIAVVLLGFGALLLSQWASGGEPLRRTVVLAFLLALSLPAWSVLLAAF
                     DTALYAAACRGRRLNGEHDEHDEQNAFQSRRADKNRRIDQRKERISMKTRDQIESTMK
                     WDLGGIYPNEDLWEQDFAAAQELVGAFSAHAGKLHVSDDALYAALTDGSKLSYLVEKL
                     YVYAHMKKDEDNGNVRYQGMTDRAIQMLVAAEAASSFVKPEILAIPHETLVSWSKQER
                     FKHFRFDLFDIDRRREHTLSTEEERLLAMAEDALSGAHNVFTMLSDVDLEYGTVTDEN
                     GEEVKLTHGTYGMFLMSPDRRVRRDAYEGLYKAYRSMKNTITATYATSVKADVFRART
                     HNFSGALEAALFGNGVPVSVYDQLIEAVHEKLPTLHKYLAIRKRLLGVDQLEMYDLYV
                     PILSDLDIPMPYEDAKSLVKEALIPLGEPYGKLLDDSYTKGWIDVYETPGKTSGAFSW
                     GVYGVHPYVLLNYQDKLDDAFTLGHELGHAMHSYYSNSAQPYETSQYRIMVAEVASTV
                     NETLMTYHLLEKETDKMKRAYYITQFLESFRTTCFRQTMFAEFERNSHRMAENGEPLT
                     VESLSEMYRKLNELYYEGAHVDDNIAIEWMRVPHFYNAFYVYQYATGLCSAVKLADDI
                     LHHGALERYLTFLKSGGSDHPIEELKSAGVDLAKKESILASLDV"
     gene            2956..>3681
                     /locus_tag="PLR57_01810"
     CDS             2956..>3681
                     /locus_tag="PLR57_01810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQQ40220.1"
                     /translation="MDRRILHADFNSFYASVACFLDPALRPHPVAVAGNPEARHGIIL
                     AKNELAKRFGVKTGEAIWQAKQKCPLLVTVKPDFESYQKFSALGRAIYGEYSDQVEAF
                     GLDENWIDVSACTETMEDARRLADVIRFRIRDELGITVSIGVSDNKVFSKLGSDLKKP
                     DAVSVISPENYKKVAWPLPASELLYVGRATQAKLRTYGILTIGDLATTPASFLKTQFG
                     KCGVMLHAFANGLDAAPVLHTVDQ"
BASE COUNT          745 a         1141 c         1072 g          723 t
ORIGIN      
        1 aacacatcca gcgaagccag aatgctctcc tttttcgcga ggtccacgcc cgcggacttg
       61 agttcctcga tggggtggtc gctgccgccg ctcttgagga acgtcaggta ccgctccagc
      121 gcgccgtgat gcagaatgtc gtccgccagc ttgaccgccg agcagaggcc ggtcgcgtac
      181 tggtaaacat agaacgcgtt gtagaagtgc ggaacgcgca tccactcgat ggcgatgttg
      241 tcgtccacat gcgcgccttc gtagtacagc tcgttgagct tgcggtacat ctccgaaagg
      301 ctctcaacgg tgagcggctc gccgttttcc gccatgcggt ggctgttgcg ctcgaactcg
      361 gcgaacatgg tttgccggaa gcacgtggtg cggaaggatt cgaggaactg cgtaatgtaa
      421 tacgcgcgct tcatcttgtc cgtttctttt tcgagcaggt ggtaggtcat cagcgtttcg
      481 ttgaccgtgg atgcgacctc cgcaaccata atgcggtact gcgaggtttc gtagggctgc
      541 gcggagttgg agtaatacga gtgcatcgca tggccaagct cgtggccgag ggtaaacgca
      601 tcgtccagct tgtcctgata gttcaaaagc acgtacggat gcacgccgta gacgccccag
      661 gagaatgcgc cgctggtctt gcccggcgtt tcgtaaacgt cgatccagcc cttggtgtac
      721 gaatcgtcca gcagcttgcc gtaaggctcg ccgagaggga taagcgcttc cttgacgagc
      781 gactttgcgt cctcgtaagg catcgggatg tccaaatccg aaagaatggg cacatacagg
      841 tcgtacatct ccagctgatc cactccgagc aggcgcttgc ggattgcaag gtatttatgc
      901 agcgtcggca atttttcgtg gacggcttcg atgagctgat cgtaaacgga aaccggaacg
      961 ccgttgccga acagcgcggc ttccagcgcg ccggagaagt tatgcgtgcg cgcgcggaac
     1021 acgtccgcct tgacggaggt ggcgtaggtt gccgtgatgg tgtttttcat gctgcggtag
     1081 gctttgtaga ggccctcgta cgcgtcccgg cggacgcggc ggtccgggct catgaggaac
     1141 atgccgtagg tgccgtgggt gagcttgact tcctcgccgt tttcgtccgt gaccgtgccg
     1201 tactccaagt ccacgtccga aagcatggta aagacgttat gcgcgccgga aagcgcgtcc
     1261 tccgccatgg cgagcaggcg ctcttcctcc gtggaaagcg tatgctccct gcggcggtcg
     1321 atatcgaata aatcaaaccg gaagtgctta aagcgttcct gcttcgacca gctcacaagc
     1381 gtttcgtgcg gaatggcgag aatttccggc ttgacgaacg aggaagccgc ctccgctgcg
     1441 acgagcatct gaatggcgcg gtcggtcatg ccctgatagc ggacgttgcc gttgtcctcg
     1501 tcctttttca tgtgcgcgta aacatagagc ttttccacaa ggtacgaaag cttgctgccg
     1561 tccgtgagcg cggcgtacag cgcgtcgtcg gagacatgca gtttgcccgc gtgcgcggaa
     1621 aacgcgccga ccagttcctg cgccgctgcg aaatcctgct cccaaagatc ctcgtttgga
     1681 tagatgccac ccaggtccca cttcatggtg ctttcaattt gatctctcgt tttcatgctg
     1741 attcgttcct tccgttgatc aattcttcta tttttatccg ctctgcggct ctgaaaagca
     1801 ttttgttcgt cgtgttcgtc gtgttcgccg tttaaccgcc gtccccggca ggccgcggca
     1861 tagagcgccg tatcgaaagc ggcgagcaga accgaccacg ccggcagcga cagcgccagt
     1921 aaaaacgcca gtacgactgt ccgccggagc ggctccccgc ccgacgccca ctgggacagc
     1981 agcagcgcgc caaagccgag cagtacgacg gcgatcagcc acggcatgta cacggcgctc
     2041 gccgtttgcc gcacgcgccg gtgcagcaaa aacccgcgcc gcagcgcgcg aaacccgcca
     2101 atattctccc gcgccgccgc ggcgagcgaa agccccagcg gcacgcgcag cagccagaac
     2161 agcgaaagtc ccagcgtgag aaccgcgctg gagcaaaacg agccgaagcg gggaatcttt
     2221 tccgcagcca gcgtcagcgg ccagcaaaaa ccggctgccg caagcccgcc gcagagccaa
     2281 acgagcgccg ccgcccagta cgcgcccttg acgcgcacat acgagcggtt tgccgaggta
     2341 aggctgcccc cgccgcgcgg agcgacaaac tgcgcttccg catacgtttc gtaaacggcg
     2401 accaccgcgc gcggatgcag gtacgcggcg tacagccccg tcagcaggat tgccggaatg
     2461 ccgcagaaga tcagcagagc ttccgcccgg aacccactgc gcacgacgcc cagcagctcc
     2521 ggctgcaatg ccgccgccgc aaggaacgcg cccgcgctca caagcaccat cagcgccagt
     2581 cccgcgtgaa gaaaaaaccg tttcagaagc gaaaaaaaac gcgcgcgcag gagctttcgc
     2641 gccgagcgcg caatgtcccg cgtggtgatt tccgtttcgt cctgcctttg tacccgattc
     2701 attcgctctg tttccgtctc tttaccggta gtatagcata cgcgcccgcc tcaaagccag
     2761 aaaacgcgcg gctttttgcc gaatttccac cgtgcagaca ctttggcgaa agcgaaaatc
     2821 cggccttgac atttttcgac aatgcggtat actatagatg cgaacaaacg tttgtgttcg
     2881 taatttcacg accattcctg cgcccgtcca acagacggta cgcaatccgg cggagagaaa
     2941 aggaggcgca ttcggttgga ccgcagaatt ctgcacgcgg acttcaatag tttttacgcc
     3001 tcggtggcgt gcttcctcga ccccgccctg cggccgcacc ccgtggccgt tgcgggtaat
     3061 ccggaagcgc ggcacggcat tatcctcgcc aaaaacgagc tggccaagcg ctttggagtc
     3121 aaaaccggcg aagcgatctg gcaggccaaa caaaaatgcc cgctgctggt taccgtcaag
     3181 ccggactttg aatcctacca gaagttctcc gcgctcggac gcgccatcta cggcgaatac
     3241 tccgatcagg tggaagcctt cgggctggac gaaaactgga tcgacgtttc cgcctgcacc
     3301 gaaaccatgg aggacgcgcg ccgccttgcg gacgtaattc gcttccgtat tcgcgacgag
     3361 ctgggcatca cggtttccat cggcgtttcg gacaacaagg tgttttccaa gctcggctcg
     3421 gaccttaaaa agccggacgc cgtctccgtc atttcgccgg agaactacaa aaaagtcgcc
     3481 tggccgctgc ccgcgagcga gctattgtac gtcggccgcg ccacgcaggc aaagctgcgc
     3541 acctacggca tcctcaccat cggggatttg gcaaccacgc ccgcttcgtt tctcaaaacg
     3601 cagttcggca agtgcggcgt catgctgcac gcgttcgcca acggcctgga cgcggcgccc
     3661 gttctgcata cggtcgatca g
//
LOCUS       DAOMGY010000071         3664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_26468_length_3664_cov_3.718759, whole genome shotgun sequence.
ACCESSION   DAOMGY010000071 DAOMGY010000000
VERSION     DAOMGY010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26468_length_3664_cov_3.718759"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLR57_01815"
     CDS             complement(<1..543)
                     /locus_tag="PLR57_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40221.1"
                     /translation="MEDTFQRFDMLAFPKRQRLRLLAWLISFPAVWQHKAVIKKIRMD
                     ELKPPYLLLCNHNAFQDFKVTTAAVFPHRANYVVAIDGFTAPTKKGFESREWLLRTVG
                     CICKRKFTNDAILVRQLNRVVKKGDIAVLYPEARYSLCGTSAILPESLGKLCQLLKVP
                     VVSLIMHGHHVNSPYWNLRER"
     gene            complement(748..1284)
                     /locus_tag="PLR57_01820"
     CDS             complement(748..1284)
                     /locus_tag="PLR57_01820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013659023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HQQ40222.1"
                     /translation="MKTILLVVDVQQALVDEGPAHKDEFLLNLNLLISTAHDYGVEAI
                     YVRHDGGEGDVLAMDTEGWQLHSSLVPRVDEKIVDKRYSSAFRKTDLREYLDGKGVKR
                     IILCGMQTEYCIDTTCKVAFEYGYDVIIPSGGTTTYTNPLLSGEKLVVYYERLIWNEP
                     LATVKPMEEVLELLKSKK"
     gene            complement(1321..1983)
                     /locus_tag="PLR57_01825"
     CDS             complement(1321..1983)
                     /locus_tag="PLR57_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HQQ40223.1"
                     /translation="MKIKIVENGPYLVSGGVPLLERIIVPKGEGYEYQNGRELPQKEV
                     YALCRCGRTSTPPFCDGAHTSCGFDGTETACRDDYDKRATLLPGTGIDLLDDGRCAFA
                     RFCHREGGDAWELSEDSASAENKSEAIIAANECPAGRLTAVEKDGRQHEPVYEPAIEI
                     LQDPEEEVSAGIFVKGGIPIESSDGTTYEIRNRVVLCRCGASKNKPFCDAAHIVEVYK
                     DK"
     gene            complement(2018..2581)
                     /locus_tag="PLR57_01830"
     CDS             complement(2018..2581)
                     /locus_tag="PLR57_01830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQQ40224.1"
                     /translation="MEQLKILAIAGSLRSQSYNRQLANLAKAAVGERAEFELLEYADV
                     PLLNQDFEYPAPEPVKRVREQVKAADGIWFFTPEYNHAVPGVLKNLIDWLSRPVSKEE
                     RQVLSGKPAAISGITTGMSGTGIAQDALVALISLLNMRVMNIPRLTIPNAAQQTNAEG
                     KLDLVASAPYLQKQADAFLRFIADQKK"
     gene            complement(2625..3461)
                     /locus_tag="PLR57_01835"
     CDS             complement(2625..3461)
                     /locus_tag="PLR57_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQQ40225.1"
                     /translation="MRLCEIAKTVRGQHAVDGAGVSLVRVLGHRDVYDFDPFLMLDSF
                     DSTDPSDYLAGFPTHPHRGIETITYLLSGKIEHEDSLGNKGVIRAGESQWMTAGSGIL
                     HQEMPKASERMLGFQLWLNLPKAEKMAEPAYLSITKEMIPVVRQGGAEIRILSGKFQD
                     TEGVTPRHIPATILDIHATYGEELTLPVKPDETTFVFLIEGDGMINGNLIESKTAVLF
                     GVGDAIRVSPTPEKCLRYICFSAKPLREPIAWGGPIVMNTREELELAFEELSRGTFIK
                     HA"
BASE COUNT          812 a          977 c          968 g          907 t
ORIGIN      
        1 gcgctcgcga aggttccagt agggcgagtt gacatggtga ccgtgcataa tcagtgaaac
       61 gacgggaacc tttaaaagct ggcagagctt gccgagcgat tcaggcaaaa ttgcgctggt
      121 gccgcagagg gaatatcgcg cttcgggata gagaaccgcg atatcgcctt ttttgacaac
      181 tcggttgagc tggcgcacca gaatcgcatc gttggtgaac ttccgtttgc agatgcagcc
      241 aacggttctc aaaagccatt cgcggctttc aaagcctttt ttggtcggcg cggtaaaacc
      301 gtcgatcgcg acgacataat tcgcgcgatg cggaaagacg gctgcagttg taaccttaaa
      361 atcttgaaaa gcgttgtgat tgcagagaag cagataggga ggcttaagtt cgtccatgcg
      421 gatcttttta ataaccgcct tgtgctgcca aacagctggg aagctgatga gccatgccag
      481 aagcctgagc cgctgacgct ttgggaacgc aagcatgtcg aaccgctgaa acgtgtcttc
      541 cactgatgct ccttttctgc ggtcaacagt tttaccgcag actgtatagt atgtgcgtat
      601 tatattggtt cacttgcggc gttgtcaatc taaacagact ttactggaag cgcgctcaag
      661 aatattgaat cgaacggttt ttcaagcaca agaaaacccg ctccacatat gaagcgggtt
      721 tgatgaaatt cgtgattcaa cgaaacgtta tttcttgctt ttcaacagtt caagaacttc
      781 ctccatgggc ttgacggtgg cgagcggctc attccagatc aagcgctcgt aatagacgac
      841 cagtttctcg ccggaaagca ggggattggt atatgttgtc gttccgccgg aggggattat
      901 gacgtcgtag ccgtactcaa acgcaacttt gcaggtcgta tcgatgcagt attccgtctg
      961 catgccgcag agaatgatgc gcttcacgcc cttgccatcg agatactctc gcaaatcggt
     1021 tttgcgaaac gcgctggaat agcgcttatc gacgattttt tcatcaacgc ggggaacgag
     1081 cgagctatgc agctgccagc cttcggtatc cattgccaga acatcgccct cgccgccgtc
     1141 atggcgaacg tagattgctt caacgccgta atcatgcgcg gtggatatca agaggttcag
     1201 gttgagcaga aattcatcct tatgcgcggg gccttcgtcc accagagcct gctgcacgtc
     1261 aacgaccaac aagatggttt tcatattaat acctcctgct actaaaaaat caagtttcaa
     1321 ttacttatct ttatacactt caacaatatg cgccgcgtca cagaacggct tattcttcga
     1381 agcaccgcaa cggcagagca ccacgcggtt gcggatttca taggtagttc cgtccgaaga
     1441 ctcaatgggg attccgccct tgacgaagat tcccgcgctg acttcctctt cgggatcctg
     1501 cagaatttcg atggccggtt catagaccgg ctcatgctga cggccgtctt tttccaccgc
     1561 ggtcaggcgg ccggcggggc actcgtttgc ggcgatgatt gcttccgact tgttttctgc
     1621 agaggcggaa tcttccgaca gctcccatgc gtctccgcct tccctgtgac agaaccgcgc
     1681 aaaggcgcat ctgccgtcgt ccagcaggtc gattcccgtg ccgggcagaa gagtcgcccg
     1741 tttgtcgtaa tcgtccctgc aagccgtctc cgtgccgtcg aagccgcatg aagtatgcgc
     1801 gccgtcgcaa aacggcgggg tggacgttct tccgcagcgg cagagcgcgt agacttcctt
     1861 ttgcggcagt tcgcgaccgt tttggtattc gtatccttcg cctttgggca cgatgatgcg
     1921 ttccagaagc ggaacgccgc cggaaaccaa gtacggcccg ttttctacga ttttgatttt
     1981 cattttgtcg atgcttttgt tcaaagtgcg ttccgcctta tttcttttga tcagcgatga
     2041 accgcagaaa cgcgtccgcc tgcttttgca gatacggcgc actggcaacc agatccagct
     2101 taccttccgc gttcgtctgc tgcgctgcgt ttggaatcgt gaggcgggga atgttcatca
     2161 cgcgcatatt gagcaggctg atgagcgcaa caagcgcgtc ctgcgcgatt cccgtgccgc
     2221 tcatgcctgt cgtaatgccg ctgatagcgg cgggcttacc ggagagcacc tggcgttctt
     2281 ctttactgac agggcgcgaa agccagtcga tcagattttt cagcacgccc gggacggcat
     2341 ggttgtactc tggcgtaaaa aaccagatgc cgtcggcggc tttaacctgt tcccgaacac
     2401 gcttaacggg ttccggagcg gggtattcga aatcttgatt caacagggga acatccgcat
     2461 actcgagcag ctcgaattca gcgcgttcgc cgaccgcagc ttttgcgagg ttcgccagct
     2521 gacgattata ggattgtgaa cggagagaac cggcaatggc aaggattttc agttgttcca
     2581 tatggtactt ctttctattt caaatcgttt tattatagga atggttatgc atgcttgata
     2641 aacgttccgc ggctgagctc ttcaaatgcg agctccagct cttcgcgcgt gttcatgacg
     2701 atcggcccgc cccaagcgat tggttcgcgc agcggctttg ctgaaaagca gatgtatcgc
     2761 aggcatttct ccggcgtggg agatacgcga atggcgtcgc caacgccaaa gagcacagcc
     2821 gtcttcgact caatcagatt gccgttgatc atgccgtcgc cctcgataag aaacacaaat
     2881 gtcgtctcat ccggcttgac cggtaaggtc agttcctcgc cgtaagtcgc gtgaatatcc
     2941 agaatcgtag ccggtatatg gcgcggggtt actccttcgg tatcctgaaa cttgccggaa
     3001 agaatgcgaa tttccgcgcc gccctgccgt acgacgggaa tcatttcctt tgtgatgctt
     3061 aaatacgcgg gttccgccat cttttccgct ttcggcaggt tcagccaaag ctggaaacca
     3121 agcatccgtt cggaggcctt tggcatttcc tgatgcagga tcccgcttcc cgcggtcatc
     3181 cactggcttt ctccggcgcg gataacgcct ttgttgccga ggctgtcctc gtgctcaatc
     3241 tttccggaaa gcaggtaggt aatcgtttca attccgcggt gcggatgggt aggaaatccc
     3301 gccaggtagt ccgaagggtc ggtggaatca aaagagtcca gcattaaaaa cggatcaaaa
     3361 tcataaacgt cccgatgccc caatacgcgc acaaggctga cgcccgcgcc gtctacggcg
     3421 tgctggccgc gcacggtttt tgcaatttca cataatcgca tattgtttcc tccttacggt
     3481 aaaagtttca tttatcgtta tcagccgaat cggtaaccgg tcatcgagag cgatcccagc
     3541 acacatgcgt cgttgaaaac ggatatatca tcttcatcca ttttcgcgcc aagcacgtaa
     3601 attaatggcg cataatgatc cggcgtagta aaggcgtagt atgcggaaga ccccgcgcgt
     3661 tcat
//
LOCUS       DAOMGY010000072         3662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_26486_length_3662_cov_4.619906, whole genome shotgun sequence.
ACCESSION   DAOMGY010000072 DAOMGY010000000
VERSION     DAOMGY010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26486_length_3662_cov_4.619906"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLR57_01840"
     CDS             complement(<1..844)
                     /locus_tag="PLR57_01840"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HQQ40226.1"
                     /translation="MKRCSVGGQAVMEGVMMRTPEGAVALAVRKADGTIVTEYTEKKA
                     KSDHPAQKWPVVRGVVAFVDSLSVGMKMTTRSAELYGEGIEEEPSRFEKWLAAKTGKS
                     AMDVAIAVAVVLAVALAVGLFVFLPSLTTQLISWKEATPRIWKSLTEGFVRLIIFLAY
                     ITVIALMKDIRRVYMYHGAEHKTIACYEHEVELTPENAMKYSRLHPRCGTNYLFLVMA
                     VSILFFAILPYTENFFLRFLTRLVFLPLVAGLAYEVRKLAAASDKLWARISRAPGLGL
                     EYLTT"
     gene            complement(1034..1231)
                     /gene="rpmE"
                     /locus_tag="PLR57_01845"
     CDS             complement(1034..1231)
                     /gene="rpmE"
                     /locus_tag="PLR57_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HQQ40227.1"
                     /translation="MKKDIHPKYGQATVRCACGETFTTGSTKDELTVEICSKCHPFFT
                     GKQKLIDSGGRVDRFKKRYGL"
     gene            complement(1371..3257)
                     /gene="rho"
                     /locus_tag="PLR57_01850"
     CDS             complement(1371..3257)
                     /gene="rho"
                     /locus_tag="PLR57_01850"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQQ40228.1"
                     /translation="MNKEQLEAKSLGDLREIAKLQGVKSLTKFRKGELVEIIMSGGIV
                     PPDFGTSEESGEEVLSSEGTAEESAKTGEPEADLSDHSSQMPDSEGGYRRSGYTPRPY
                     QQKPYYDNRPQQGGYQRRQYNPQQGGYQQGGYQQRGYQQGGGYQQQGGYQQQGGGYQP
                     QGGGYQQQNGYQPQGGYQGQGGGYQPQGGGYQPAYSPSSYGADQGAGFEDDRRFRPRA
                     EGYYNKDYGTSNPAVPEILQTGDCGDAEGVLEVLPDGYGFLRSENYLSGQHDVYVSIA
                     QIRRFNLRTGDMVTGKTRPSKEGERFLALLYITAVNGESPDTIAERKPFEELVPVFPF
                     ERYTLELPGTNSLAIRLIDLIAPIGKGQRAMIVSQPKAGKTTLLKSIANGISTNYPEA
                     HLIVLLIDERPEEVTDMQRSIKGEVLYSTFDELPEHHTRVAEMVQERAMRLVEMGKDV
                     VILLDSLTRLSRAYNLTIPPTGRTLSGGMDPGALHKPKRFFGAARKIEGGGSLTIIAT
                     ALTETGSRMDDMVYEEFKGTGNMEIHLDRKLSERRIFPAIDIYKSGTRREDLLLSTEE
                     LEAVRTIRRVLSGGNPAEVTEHLISMLEKTSTNDEFLQRLKDWVNIYEKDGYTTMSTS
                     RFGK"
BASE COUNT          781 a         1017 c          934 g          930 t
ORIGIN      
        1 gggtggtcaa gtactccagt cccagtcccg gcgcgcggct aattctcgcc cagagcttgt
       61 cggacgcggc ggcgagcttg cgcacctcgt atgcaagccc cgcaaccagc ggcagaaaca
      121 ccagacgcgt caaaaaccgc agaaagaagt tttccgtata cggcagtatt gcaaagaata
      181 gaatggaaac cgccatgacc agaaacaggt agttcgttcc gcatctggga tgcaggcgcg
      241 aatacttcat tgcgttttcc ggcgtcagtt ccacttcatg ttcgtagcag gcgatggtct
      301 tgtgctccgc accgtggtac atatacacgc ggcggatatc cttcatcagc gcgataacgg
      361 tgatgtacgc taaaaagatg atgaggcgca caaacccttc cgtcaggctc ttccagatac
      421 gcggggtcgc ttccttccag gagatcagct gcgttgtcag cgaaggcaaa aatacgaaga
      481 ggcctaccgc gagcgcaacc gcaagaacca ccgcaacggc aattgcaacg tccatggcgg
      541 attttccggt ctttgcagcc agccattttt cgaaccggct cggctcttct tcaattccct
      601 cgccatagag ctctgcagag cgggtggtca tcttcatacc aacggaaaga gaatcgacaa
      661 acgcaacaac accgcgcacc accggccact tctgggcggg atggtcgctc ttagccttct
      721 tctccgtgta ctccgtcacg atggtgccgt cggcttttct taccgcaagc gccacggcgc
      781 cttcgggcgt acgcatcatg acgccctcca tgaccgcctg gccgccgacc gagcatctct
      841 tcatgtattg ttcctcttgt ttccgacgca tctatgaaaa aagcgataaa agacacatct
      901 gtttgttctg tcagtttacc atatttaggg gttacgcaca aggttgaacc atacagttgg
      961 ttgtgcgctt cccggtgtga tttcagctga acaagaaaaa acctcccctg aaacgggaag
     1021 gtttttacgt ttcttacaga ccgtaacgct ttttaaagcg atccacacgg ccgccgctgt
     1081 cgataagctt ctgcttgccc gtgaaaaacg ggtggcactt gctgcagatt tcaacggtca
     1141 gctcatcttt cgtagagccg gtcgtaaagg tttcaccgca agcgcagcga accgtggctt
     1201 ggccgtactt cggatggata tccttcttca tgctcttcac tccctttaac agactaccat
     1261 cggctattat aaccatagaa gtctgaaaat gcaactgatt tttcacgatt ttcgcaaaat
     1321 atcacgattt cttttccgca aaaatcgccc gcgtgcgggg aaaaatgacg ttatttgcca
     1381 aaacggctcg tggacatggt cgtatagccg tccttttcgt aaatgttcac ccagtctttc
     1441 agccgctgga ggaattcgtc gttggttgaa gtcttttcca gcatgctgat gaggtgctcc
     1501 gtaacttccg caggattccc gccggaaaga acgcggcgga tggtgcgaac cgcttccagc
     1561 tcttccgtcg agagaagcag gtcctcgcgg cgcgtgccgg acttgtagat atcgatggcg
     1621 gggaaaattc tgcgttcgct gagcttgcgg tcaagatgga tttccatgtt gcccgtgccc
     1681 ttgaactcct cgtacaccat gtcgtccatg cggctgcccg tttcggtcag cgcggtggca
     1741 ataatcgtca gactgccgcc tccttcaatt ttacgcgcgg cgccgaagaa gcgcttgggc
     1801 ttatgcaacg cgcccggatc cataccgccg gagagcgtcc gccccgtcgg aggaattgtc
     1861 aggttgtatg cacgggaaag cctagtgagg ctgtccagca gaataacgac gtccttaccc
     1921 atctccacaa gacgcatggc gcgttcctgt accatttcag ccacgcgggt gtggtgctcg
     1981 ggcagttcgt cgaaggtgga atacagcact tcgcccttga tggagcgctg catatccgtc
     2041 acttcctccg gccgctcgtc gatgagcagc acgatgaggt gcgcttccgg atagttcgtt
     2101 gaaatgccgt tggcaatgct cttgagcagt gtcgttttgc ccgctttcgg ctgggaaacg
     2161 atcatggcgc gctggccttt gccgattggc gcaatcaggt cgatcaaccg gattgcaaga
     2221 ctgtttgttc cgggaagttc gagcgtatac cgttcgaacg ggaacacggg aaccagttct
     2281 tcaaaaggtt tgcgttccgc aatcgtgtcc ggcgactcgc cgttgacggc ggtgatgtat
     2341 aacagcgcga ggaagcgctc gccctctttg gaaggacgcg ttttaccagt gaccatgtcg
     2401 ccggtgcgca ggttgaatct tcgaatctgc gcgatggaaa catacacgtc atgctggccg
     2461 gagagatagt tttcgctcct taagaagccg taaccgtccg gcaggacttc cagaacgcct
     2521 tccgcatccc cgcagtctcc ggtctgcaga atctccggca cggcaggatt gctcgttccg
     2581 taatctttgt tgtagtagcc ttccgcgcgg ggacgaaaac ggcggtcgtc ttcaaacccc
     2641 gctccctgat ccgcgccata gctgctcgga gaatatgcgg gctgataccc gccgccctgc
     2701 ggctgataac cgccgccctg gccttgataa ccgccctgcg gctgataacc gttctgctgc
     2761 tgatacccgc cgccctgcgg ttgatatccg ccgccctgct gctgatagcc gccctgctgc
     2821 tggtaaccgc cgccttgctg atagccgcgc tgctggtaac cgccctgctg atatccgcct
     2881 tgctgcggat tgtactgtct tctctggtat ccgccctgtt gcggacgatt atcataataa
     2941 ggcttctgct ggtaggggcg cggcgtataa ccggaacggc ggtaaccgcc ttcggaatcc
     3001 ggcatctggg aagaatgatc cgaaagatcc gcttccggtt ctcccgtttt tgcggattct
     3061 tctgctgtgc cttcggatga aagcacttcc tctccacttt cttcggacgt gccgaaatcg
     3121 ggtggtacga tgccgcccga cataatgatt tctaccaact cgccttttcg aaatttcgta
     3181 agcgatttta caccttgtaa ttttgcgatt tcacgcaaat caccaaggct cttggcctct
     3241 aactgttctt tgttcaactg taaactcctc tcttacggta agaaacgtta cctgtcagca
     3301 ccattaatcg gaataaaaga tgtgatttct ttgatgagga aatcatatcc ttgggcactc
     3361 tttttgtcaa ggcgcgccgc ctttttttca caacatttcc gctattgggc gttgttttgg
     3421 gtctttcatg ttggaaactg ttcccattgg tattgcgcgc gccgcaaatt gcaatccacg
     3481 ctcttgtaaa aaagcggacg gatttttgaa attcattttg tttttcaaat gcaattcaaa
     3541 gtattataaa cgcttccggc ggattaatcc agatgaattt ttatcatttt agcagataaa
     3601 ttatcagaac gagttaattt catgttatat ttttgtgaca atgctcggaa agagcatttc
     3661 at
//
LOCUS       DAOMGY010000073         3650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_26611_length_3650_cov_3.840334, whole genome shotgun sequence.
ACCESSION   DAOMGY010000073 DAOMGY010000000
VERSION     DAOMGY010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26611_length_3650_cov_3.840334"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1156)
                     /gene="ftsW"
                     /locus_tag="PLR57_01855"
     CDS             complement(11..1156)
                     /gene="ftsW"
                     /locus_tag="PLR57_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HQQ40229.1"
                     /translation="MERRPLGRKRQRIGHIDYPIVLMVGLLCAFGLVMVFSASYYYAQ
                     NTASVGYDGYYFIRKQAMYLAIGFPLMLLISLIDFRKLEKFKVIAILISIILLVAVLI
                     FGQETNGAKRWIVVGGQSIQPSEIAKFGMMLYMCSFMARKHAIMQNFSKGMLPMLLII
                     GVICGLVMLQPNMSMAVIMGLMGYALLFIGGCDTKQMLLLLVVLIGMFALLAVIEPYR
                     MARLTSFVNPWADALGDGYQLIQSYYALGSGGLFGVGLNNSHQKLLYMTYGESDFIFA
                     IVCEELGLIGGLLVIAAYGFIIYRGIRIALQCRDRFGSMLAGGITVVFGLQVFVNIGV
                     CTGLLPTTGQALPFISAGGSSMMIFLAAMGVLLNISRFNNMQKPAKA"
     gene            complement(1649..2611)
                     /locus_tag="PLR57_01860"
     CDS             complement(1649..2611)
                     /locus_tag="PLR57_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ40230.1"
                     /translation="MKQKYPIDEHLKVPVNGTTLDLRIRGTNTNNPVVLFLHGGPGIC
                     DRHFVLRDQAPLADVCTLVCLDQRGSGKSYTREQGKREMSVDQVVEDARIVVEFLCDK
                     FRKDKIYLVGHSYGSFLGVLLCQRYPKHIAAYVGLGQLADGPENERISYEFVWNEAQK
                     RGDAKAIADLTRIGAPVHGFYKSLDDLTVQRNYMTKFGGAAHGESESIFTSMVLPVLR
                     SPEYTLFDLIKYANGAYYNLRQLWESVIACEFIKTVRSLDMPVFITQGKFDRNTPPEL
                     AKAWLDALDAPKKEWIWFENSAHSPIREESARWNEVFREKVLTC"
     gene            complement(2711..3010)
                     /locus_tag="PLR57_01865"
     CDS             complement(2711..3010)
                     /locus_tag="PLR57_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40231.1"
                     /translation="MAKTVCGGLEQPHQDKTKLSAFLSERGKEHAPHGKQRTNLPRSA
                     RLLTRLFVRTELVRAALSGWRYYSMFTMRGQPREESRIFLYERQKELYNTIDSFK"
     gene            3054..>3650
                     /locus_tag="PLR57_01870"
     CDS             3054..>3650
                     /locus_tag="PLR57_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ40232.1"
                     /translation="MNLKPKLRFAPLALAMLLLLFSLGCALPSPVPLEDGSVVQTEPT
                     YDNTTQNQTYLIDMIDMAGNPVQMNSYAGSIVVLDPGDCEILCAIGAEEQIIGRSEDC
                     DYPESVASLPVVTTEGMANTEQILTLNPQAVIMDAEAATDTSLIAELKEANITPIVTN
                     VTDIKGLYTAITLLGTVTDRVAESGSVVANLLAAFAGIQ"
BASE COUNT          851 a         1038 c          972 g          789 t
ORIGIN      
        1 atttgagtcc ttacgctttc gccggtttct gcatgttgtt gaaacgcgaa atattgagca
       61 ggacgcccat tgcggcaagg aagatcatca tcgaggagcc gccagcgctg atgaacggga
      121 gcgcctgtcc ggtcgtcggg agtagaccag tgcagacgcc gatgttaacg aaaacctgca
      181 ggccgaaaac caccgtaatg ccgcccgcaa gcatgctgcc gaatcggtct ctgcactgca
      241 gcgcaattcg aatgccgcgg tagatgatga atccgtacgc tgcgatgacg agcagcccgc
      301 cgatgagccc cagctcctcg cagacgatgg cgaagataaa gtccgactcg ccgtacgtca
      361 tgtacagcag cttttgatgg ctgttgttga ggccgacgcc gaacagcccg ccggaaccca
      421 gcgcgtaata ggactgaatc agctggtaac cgtcgcccag cgcgtcggcc caagggttga
      481 caaacgaagt cagacgcgcc atgcggtaag gttcaataac cgccagcaac gcaaacatac
      541 cgataagcac gacaagcagc aacagcatct gcttggtatc gcagccaccg ataaacagca
      601 gcgcgtatcc catcaggccc atgataacgg ccatggacat gttgggctgc agcatgacca
      661 ggccgcagat aacgccgatg atcagcagca tgggcagcat gcccttggag aaattctgca
      721 tgattgcgtg ttttcgcgcc atgaacgagc acatgtagag catcataccg aatttcgcga
      781 tttcggaagg ctgaatactc tggccaccga caacgatcca acgcttcgcg ccgttggtct
      841 cctgaccgaa aataagcacc gccacgagaa ggatgataga aatcagaata gcgatgacct
      901 tgaatttttc cagctttcta aaatcgatca gcgaaatgag cagcatcagc ggaaagccga
      961 tggcaagata catggcctgt ttgcggataa aatagtaccc gtcgtagcca accgaagcgg
     1021 tgttctgcgc gtaataatag ctcgcggaaa ataccataac gaggccgaac gcgcaaagca
     1081 gcccgaccat caaaacgatg ggatagtcga tatgtccgat tctttgtctc tttctgccaa
     1141 gagggcgtcg ttccatcgta tctccgtttt catgtatttc ccgtcctgcc ggggtagtgt
     1201 ttcaaaaaaa atcacccgtt ttcaagctgt cggtttttac gcgcgccgtt caacaaaggt
     1261 gcatcgtgta tcataccaaa atggccgcaa actgacaagc cgccggagcc acgggggtcg
     1321 atttgttttc agaatctttc caatatggta atctttgcgg cagaattcaa gacaatcgct
     1381 ccactttcgt aactttttca aaacgaaaag ggagaacgca aagcgtcctc cttccttgtg
     1441 catcgacaaa ctccatcaga gggtaagggg gattcgcaaa accccctgat ttcaatcaaa
     1501 ttcagcgatt cattcgttga atttgtggaa aaatggcgcg attagcgcta tttttacgca
     1561 cagaaaaacc catcgataaa gcctgcaagg ctttgtcgat gggctgaaaa agagaacgca
     1621 aagcgttctc ttttttaaac atccccactc agcaggtgag cactttttcg cggaacactt
     1681 cgttccagcg cgcggactcc tcccggatgg gcgaatgcgc gctgttttca aaccagatcc
     1741 attccttttt cggcgcgtcc agcgcgtcca gccaggcttt tgcaagttcg gggggcgtgt
     1801 tgcggtcgaa cttgccttgc gtaataaaca ccggcatatc cagcgagcgc acggttttga
     1861 tgaactcgca ggcgatgacc gactcccaga gctggcgaag gttgtagtag gcgccgttgg
     1921 cgtacttgat cagatcgaac agcgtatact ccggcgagcg gagcaccggc agcaccatgg
     1981 aggtaaagat ggactcgctt tcgccatgcg ccgcgccgcc aaacttggtc atatagttgc
     2041 gctgcaccgt cagatcgtcc agcgacttgt agaagccgtg caccggcgcg ccgattcgcg
     2101 ttaagtccgc aatcgccttg gcgtctccgc gcttctgcgc ttcgttccag acgaactcat
     2161 acgaaatccg ctcgttctcc ggcccgtccg caagctgacc gaggccgaca tacgccgcga
     2221 tatgctttgg ataccgctgg cagagcagca caccgaggaa gctgccgtag gaatgcccca
     2281 cgaggtaaat cttatccttg cggaacttgt cgcatagaaa ttctactacg atgcgcgcgt
     2341 cctccaccac ctggtcgacg ctcatctcgc gtttcccctg ctcgcgcgta tagctcttgc
     2401 ccgaaccgcg ctggtccagg caaaccagcg tgcagacgtc cgcaagaggc gcctggtcgc
     2461 gcagaacgaa atggcggtcg cagatgcccg gcccgccgtg gagaaacagc actacagggt
     2521 tgtttgtgtt tgtgccgcgg atgcgcaggt ccagcgtcgt gccgttgacc ggcaccttca
     2581 ggtgctcgtc gatggggtat ttctgtttca taggcggttt gctcctcgct cgtttgattc
     2641 gatcgacggc gaacaaagtc agccgatcgt tggttcggcc ggtgctgtcc gatatagatt
     2701 gtcaagcagg ttacttgaaa ctgtctatgg tattatacaa ttctttttgc cgttcgtaca
     2761 gaaaaatgcg cgattcttcg cgcggttgac ccctcatggt gaacatgcta taatagcgcc
     2821 agccggagag agcggcgcgt accagctcag tgcgtacgaa aagccgcgtc aagagccgcg
     2881 cggaacgcgg caggttcgtt ctctgttttc catgcggcgc atgttctttg cctctctccg
     2941 acaaaaacgc ggagagtttt gttttatctt gatgcggctg ttccagcccg ccgcaaactg
     3001 tctttgccat tgcgtcccct cggctgacca aagccggaaa ggatacgaac tatatgaact
     3061 taaaacccaa actgcgcttt gcgccgctcg cgcttgccat gctcctgctg ctcttttcgc
     3121 tcggctgcgc actgccttcg cccgtgccgt tggaagacgg ctcggttgtt caaaccgaac
     3181 ccacttacga caacaccacg cagaaccaaa cgtacctgat cgacatgatc gatatggcag
     3241 gcaacccggt gcagatgaac tcctatgccg gaagcatcgt cgttttggat ccgggcgact
     3301 gcgaaatcct ctgcgccatc ggcgcggagg agcagatcat cggccgaagt gaggactgcg
     3361 actatcccga atcggtcgcg tcgcttccgg tcgtcacgac ggaaggaatg gcgaatacgg
     3421 agcagatcct tacgctcaac ccgcaagccg tcattatgga cgccgaagcc gctacggata
     3481 ccagcctcat cgccgagctt aaggaagcga acatcacgcc gattgtgacc aacgtaaccg
     3541 atatcaaggg tctctacacc gcgataacgc ttctgggaac ggtgaccgac cgcgttgccg
     3601 aatccggctc ggtggttgca aaccttctcg ccgcctttgc gggaattcag
//
LOCUS       DAOMGY010000074         3646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_26658_length_3646_cov_3.104149, whole genome shotgun sequence.
ACCESSION   DAOMGY010000074 DAOMGY010000000
VERSION     DAOMGY010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26658_length_3646_cov_3.104149"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="PLR57_01875"
     CDS             <1..1123
                     /locus_tag="PLR57_01875"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HQQ40233.1"
                     /translation="PQNAEAYLTAAGALLALNEQNEQEMKRILALGLTNAPESAEAIT
                     SWQDENGLGAAQKAAFVPDYTDVSQINTEGTTPGNLTNAMKRGDTWMGGLVTSQAGWV
                     YFSRIDNGYALYKMRADGSGLMALSSVHAVALNVAGDWLYFANVDDGKCPYRMRTDGT
                     DLEKLADFSCGFLSVEGDWIYCDASTEEYFHLSKLKIDGSEETVLFDGPIIFCCVYDG
                     YVYFDIKSMDEGGFLRIGIDGTGKQQLLTSMPSFYCIVNDRIYFTEESDPTLIKSMNL
                     DGSDITEVYRANDTVVAVNISGKLLILAKGISYDNDGLTLSGAIQIVSLDTGEVVREW
                     ETTTEPLSVAEGLLFFTEDQENMAWSCINLETFETIPTK"
     gene            1153..2529
                     /locus_tag="PLR57_01880"
     CDS             1153..2529
                     /locus_tag="PLR57_01880"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HQQ40234.1"
                     /translation="MNTKLHKSVFPLICTLALAFLLCACAKPTPVAAPEITATPVVSS
                     EPDVETIVFTDPVLEAKIRADLGQPEGDITSAQAAELLELDLSGDGTAGGSITDLSAL
                     RYFTGLFRLNLSNNAISNLEPLSGLTQLRILDLAQNPITNIEALSGLSLAKLVLSGCV
                     VTDPSPLSEMAQLRVLDLSGNAVTDVSALAGMPRLETLLLAGNPVADYRPLSVIYPEL
                     TKKDFEIVFDTDVIVFTDPVLEERIRVETGMLEGDITVADAKTVTELNLGVQQGNTEQ
                     QYHDISPLRFFVNLTKLHLDYALIYDNGNPDVSALASLTRLKELGFPGCSVADISFLS
                     GMKDLEWLDFQLNSVEDISPMAGLKKLKLLSMCSNPVTDISPLSSLTSLEELFLFGVH
                     ASDFGPLSSLGNLVFLDLCGTGITDLTPLSGLTNLKTLQLGGNPIEDYSPLAGIYPNL
                     ENKDFTIR"
     gene            2765..>3646
                     /locus_tag="PLR57_01885"
     CDS             2765..>3646
                     /locus_tag="PLR57_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQQ40235.1"
                     /translation="MPQRKSNIYKTNLSIIAIALMAVITVASMFVYNGLINRTFKADT
                     AGNTYYTYHFALVAEAHDDPFWINVWTAANKEANRMDACVEWIGHSLAEQYPLTDQLE
                     MAIAARVDGILIQPDGSDEVKQLIQDAIDARIPVITVMTDSAGSNRQGFVGYNSYDLG
                     QLYGQQVRSAMAQSPNECHAVVLFSSDVAENTQNIVYSSIMESLEGQNITVDVLVIDN
                     SNVFSAEEAIRELVVTPAESSSAGSVSGGSTQMTLKSPPDILVCLNATNTICAYQAVV
                     DHNKVGEIQILGYYDSEE"
BASE COUNT          819 a         1054 c          997 g          776 t
ORIGIN      
        1 gccgcaaaac gcggaagcct acctgaccgc ggcgggcgcg ctgctggcac tcaacgaaca
       61 gaacgagcag gagatgaagc gcattcttgc tctggggcta accaacgcgc cggaaagcgc
      121 ggaggcgatc accagctggc aggatgaaaa cggccttggc gcggcgcaga aagcggcgtt
      181 tgttcccgat tacaccgacg tcagccaaat caatacggaa ggcactacac cgggcaacct
      241 gaccaacgcc atgaagcggg gcgacacctg gatgggtgga ctagtgactt cgcaggcggg
      301 ctgggtctac ttttcgcgca ttgacaacgg ttatgcgctc tacaagatgc gcgcagacgg
      361 cagcgggctt atggcgcttt ccagcgtgca cgcggttgcc ttgaacgtcg ccggcgactg
      421 gctgtacttt gccaacgttg acgatggcaa gtgcccgtac cgtatgcgca cggacggcac
      481 cgacctcgaa aagctcgcgg acttctcctg cgggttcctc tccgtcgagg gcgactggat
      541 ctattgcgac gcaagcacgg aagagtattt ccatctcagc aaactcaaga tcgacggttc
      601 ggaagaaacg gtactttttg acggtccaat catcttctgc tgcgtttatg acggatacgt
      661 ctattttgac atcaagagca tggacgaggg cggattcctg cgaattggaa tagacgggac
      721 tggcaagcag cagttgctga ccagcatgcc atccttttat tgcatcgtaa acgaccgaat
      781 ctactttacc gaagaatccg atccgacttt aatcaaaagc atgaatcttg acggaagcga
      841 cataaccgaa gtgtaccgtg cgaacgatac ggtcgttgcg gtcaatatct ccggcaagct
      901 cctgatcctt gcaaagggca tttcctatga taacgacggg ctaaccctga gcggagccat
      961 ccagattgtc agcctcgaca ccggcgaggt cgtccgcgag tgggaaacga caaccgaacc
     1021 gctcagcgtg gcggaagggc tgctcttctt tacggaggat caggaaaaca tggcctggtc
     1081 gtgcatcaac ctggaaacgt ttgaaaccat accgacgaag tagcgtcggc ataaagccgg
     1141 gagggctcaa aaatgaatac gaagctgcat aaaagtgtgt tccccctgat ttgcaccctt
     1201 gcgctggcgt ttttactctg cgcctgcgcc aaacctacgc cggtcgcagc ccccgagatt
     1261 acggcgacgc ctgtcgtttc gagcgagcct gatgtcgaaa cgatcgtgtt taccgatcct
     1321 gtgctggaag cgaagatccg cgccgacctc ggccagccgg agggcgacat cacttccgca
     1381 caggcggcgg agttgctgga gctggacctg tccggcgacg gcacggcggg cggctcaatt
     1441 accgatcttt ccgcgctgcg gtatttcacc ggcctgttcc ggctgaacct ttccaacaac
     1501 gcaatatcga accttgagcc cctttccggg ctgacacagc tgcgcattct ggatttggcg
     1561 caaaacccga taacaaacat cgaggccctg tccggcctga gtctggcaaa gcttgtgctc
     1621 agcggctgcg tggtcaccga cccgagcccg ctttccgaaa tggcacagct gcgcgtgctc
     1681 gatttgagcg gaaacgccgt aacggacgtg tcggcgcttg ccggtatgcc ccggctggaa
     1741 acgttgctgc ttgcgggcaa cccggtcgcg gactaccgcc cgctttccgt aatctatccg
     1801 gaactcacga aaaaagactt tgaaatcgta ttcgataccg acgtaatcgt gtttaccgat
     1861 ccggttttgg aagagcgcat ccgtgtggaa accggcatgc tggaaggcga catcaccgtt
     1921 gcggacgcaa agacggtgac cgagctgaac ctcggcgtcc agcagggcaa cacggaacag
     1981 caataccacg acatttcgcc gctccggttc tttgtgaatc tgaccaagct gcaccttgat
     2041 tacgcgctta tttacgacaa cggaaatccg gacgtttccg cgctcgcctc gcttacgcgc
     2101 ctcaaagagc tcggctttcc gggctgctcg gttgcggata tctcgtttct ttccggcatg
     2161 aaggatctgg aatggctcga tttccagttg aactccgtgg aggatatctc ccccatggct
     2221 gggctgaaaa agctgaagct gctctccatg tgcagtaatc ccgttacgga catctccccg
     2281 ctttcaagtc tgaccagcct tgaggagctt ttccttttcg gcgtacacgc ctcggatttc
     2341 ggtccgctga gttcgctcgg caatctggtc tttctggatc tctgcggcac gggcattaca
     2401 gaccttaccc cgctttccgg gctgacaaac ctgaaaacgc ttcagcttgg cggcaacccg
     2461 attgaggact attcgccgct tgcgggaatc tacccaaacc tcgaaaacaa ggatttcacg
     2521 attcgctaga aatcatcact cgctcacaaa agcgcgcccg tttagggtgc gctttttttg
     2581 cgtcaattgc gccatcgtgc aatcttttta aaagtatgct atcatctttt caagcgccac
     2641 agcacctttt agggaccatt cgcagcatat ggcgcgggca tatctattct tacgattctg
     2701 gctgggtgca gttttgacga acaagaagaa ccgacttttt tcgattgcca gcaggttttc
     2761 cgacatgccg cagagaaaat cgaacatcta caaaacgaat ctttccatta ttgccattgc
     2821 actgatggcg gtcatcaccg ttgcgagcat gttcgtctac aacggcctga tcaaccgcac
     2881 gttcaaggcg gatacggcgg gcaacacgta ctacacttac catttcgcgc tggtggcgga
     2941 agcgcacgac gacccgttct ggatcaacgt ctggacggcc gccaacaagg aagccaaccg
     3001 catggacgcc tgcgtagagt ggattggtca ttcgcttgcg gagcagtacc cgctgacgga
     3061 tcagctcgaa atggccattg ccgcgcgcgt ggacggcatc ctcatccagc cggacggttc
     3121 ggacgaggtc aagcagctca ttcaggatgc gatcgacgcg aggattcccg tcatcaccgt
     3181 gatgaccgat tccgcgggaa gcaaccgtca gggctttgtc ggttacaaca gctacgattt
     3241 aggccagctc tacggccagc aggtgcgttc ggctatggcg cagtcgccca acgagtgcca
     3301 cgcggtggtg ctcttcagca gcgacgttgc ggaaaacacc cagaatatcg tctattctag
     3361 catcatggag agtctggaag gccagaatat cacagtggac gtgctggtca tagacaactc
     3421 caacgttttc agcgccgagg aagccattcg cgagctcgtc gtcacccctg ccgaaagcag
     3481 ctcggctggc tcggtttccg gcggctcaac ccagatgacg ctcaaatccc caccggatat
     3541 cctcgtctgc ctcaacgcaa ccaacaccat ctgcgcctat caggcggtgg tcgatcataa
     3601 caaagtcggt gaaatccaaa tcctcggcta ttacgattcg gaggaa
//
LOCUS       DAOMGY010000075         3627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_26851_length_3627_cov_2.751120, whole genome shotgun sequence.
ACCESSION   DAOMGY010000075 DAOMGY010000000
VERSION     DAOMGY010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26851_length_3627_cov_2.751120"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PLR57_01890"
     CDS             <1..1270
                     /locus_tag="PLR57_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HQQ40236.1"
                     /translation="PEEWFIGSYLPDFTNPKTRSWWLEKRRYLVEQLGVAGFKTDGGE
                     FLFNTEARLFDGQTGSAAHNAYPGAYEGAYHAFLDETRGEGQGVLFSRAGFTGAQKSP
                     IHWAGDQVSTFNELKAQLKAGLSLGLSGVPFWGFDIGGFAGELPSAELYLRSAAFGAF
                     APVMQFHSEPRNGQYYMTDRKAWNNDRSPWNLASVQEDERILTVYRLFANLRMNLLPY
                     LWQEAKHCAKTTRPMMAHLIFDFAKTDLESVREIEDEYMLGRSLLVAPVIQEGAASRS
                     VYLPSGSWRDFYTGELFSGGQTVEVECGLDRIPVFVRDNTAIPVNANGALVIGSEGKD
                     GAVSNRTDAYEQLGFLLYGEPKEICFADDLGSDFTLTSGEPGYALSGRLVNSFALIDV
                     TRLNAESCSAKVFGRLVPVTKIPQNTENNA"
     gene            1267..3111
                     /locus_tag="PLR57_01895"
     CDS             1267..3111
                     /locus_tag="PLR57_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HQQ40237.1"
                     /translation="MKERYLVTGNAVVSLPTIRERDAAIEGVSFLHMGAKGMIELCGD
                     EADPLLQPFVRQNGEYVPLTELRWTRHCDWIPSFTARAGEFSLEGTFLAPIGERGFGI
                     RLRLTNDGESTSAAFGLNGCWAVSKHTINESKPVTGERFVYNSGWNHSFVLDQRAGFS
                     LFAFAPIVTETPETCPITGSFERSDERITFSLVREESMEPGQTLEAVFWFGIGFEEVA
                     AATSAKEMLRRGFDFEWNKTLDWLQARRRTVGDAKLDELLNLNLLFSFFFGGGLTLDT
                     EEYALVTSRSPRYYVSAAYWDRDSLLWSFPAILLIDPPTAREMLQYVFTRQIRNIGVH
                     SRFIDGTLLEPGFELDELCAPVIALNRYIKTTGDTALLQKECVREGIEHILARLRSMR
                     HASVALYETFLQPTDDLIEHPYLTYDNVLVWYSLLALAEMFSNVWPQGRIKEMEREAE
                     QVKRAVWEHCVFEKDGEDIFAWSVDLNGKWNIYDEPPGSLLLLPYYGFCGENDPVWQA
                     SARTIRRADYPYSFADCPIAEIGCPHAPYPWVLSISNSLLSGRREQAKQHLLRTQMDN
                     GVACESVDPYTGECRTGEAFATCAGFLAYAINEAFGGKKVQNDTPSNR"
     gene            3129..>3627
                     /locus_tag="PLR57_01900"
     CDS             3129..>3627
                     /locus_tag="PLR57_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40238.1"
                     /translation="MEWIKIAPWDISCEGAEEETRDYRESVFSQGNGYLGMRGYRPDE
                     ASPNQAHRTTFYSGFYEYIRPGITDLVNQPDCSYTEIRLSGKPAREMRVSEAKQSFSM
                     KTGEASWQYMLEDGEGRKTQVESFRFASMDNRRLVALRYRITPLNYEGTLELTTGVDG
                     GVENLP"
BASE COUNT          763 a         1040 c         1124 g          700 t
ORIGIN      
        1 tcccgaggaa tggttcatcg gttcctattt gccggatttc accaacccga aaacccgctc
       61 ctggtggctg gaaaagcggc ggtatctggt cgagcagctc ggcgtagccg ggttcaagac
      121 cgacggggga gagtttctct tcaataccga agcgcggctc tttgacgggc agaccgggag
      181 cgcggcgcat aacgcctatc ccggcgctta tgaaggcgct taccatgctt ttctggacga
      241 aacgcgcggc gagggccagg gcgtgctgtt cagccgcgcg ggttttacgg gcgcgcagaa
      301 atcgcccatt cattgggcgg gcgatcaggt ttccacgttc aacgagctga aagcacagct
      361 caaagcaggc ctgtcccttg gtctcagcgg cgtgccgttc tgggggttcg atatcggcgg
      421 gtttgccggc gagcttccct cggcggaact ctacctgcgt tcggcggcgt tcggcgcgtt
      481 cgcgccggtg atgcagtttc attccgagcc gcgcaacggc cagtactaca tgaccgaccg
      541 caaggcctgg aacaacgacc gttcgccttg gaacctcgcc agcgtgcagg aagatgagcg
      601 catcctcacg gtctacaggc tgtttgccaa cctgcgcatg aaccttttgc cgtatctctg
      661 gcaggaagcc aagcactgcg ccaaaacgac gcgcccgatg atggcgcacc tgattttcga
      721 ctttgccaaa accgacttgg agtccgtccg cgagattgag gacgaataca tgctggggcg
      781 cagcctgctg gttgctcccg tgatacagga aggcgcagcg agccggagcg tgtatctgcc
      841 gagcggaagc tggcgggact tttacaccgg agaattgttc tccggcgggc agaccgttga
      901 agtggagtgc gggttggatc gcattccagt gttcgtgcgg gacaacaccg ctatcccagt
      961 caacgcaaac ggcgcgctcg tcatcggcag cgaaggcaaa gacggcgcgg tttccaaccg
     1021 cacggacgct tacgaacagc tcgggttcct gctctacgga gagccgaaag agatttgctt
     1081 tgcggacgac ctcggcagtg attttacgct gacgagcggc gaaccaggct acgcgctgtc
     1141 gggcaggctg gtgaacagct tcgcgctgat cgacgtgacc cgcttgaacg cggaatcctg
     1201 ctcggcaaag gtgttcggcc gtcttgtgcc cgttacaaaa attccgcaga atacggagaa
     1261 caacgcatga aagaacgcta tcttgtcacc ggaaacgccg tcgtctccct gccgacgatt
     1321 cgggagcgcg acgcggcaat cgaaggcgtg agctttctgc atatgggcgc aaagggcatg
     1381 atcgagctct gcggcgacga agcagatccg ctgctccagc cgttcgtccg ccagaacggc
     1441 gagtatgtgc ctttaacgga actccgttgg acacgtcact gcgactggat tccctcgttt
     1501 accgcacggg cgggcgaatt ttcgctggaa ggcacgtttc ttgcgccgat cggtgaacgg
     1561 ggcttcggca tccggctacg gctgaccaac gacggagaat cgacgagcgc ggcgttcggg
     1621 ctgaacggtt gctgggcagt cagcaagcac acgatcaacg aatccaagcc ggtcaccggc
     1681 gaacggtttg tttacaacag cggctggaat cacagctttg tgctcgacca gcgcgcgggt
     1741 ttttcgctct ttgcgttcgc gcccatcgta accgaaacgc ctgaaacctg cccgattacc
     1801 ggctcgttcg agcggtcgga cgaacgaatt accttttcgc tcgtccgaga ggaaagcatg
     1861 gagccgggac agacgctgga agcggtgttc tggtttggga ttggcttcga agaggtcgcg
     1921 gcggcaactt ccgccaagga gatgctccgc cggggattcg actttgaatg gaacaaaacg
     1981 ctggactggc tgcaggcgcg caggcgtacg gttggcgacg ccaagctgga cgaactgctg
     2041 aacctgaacc tcctcttcag cttcttcttt ggcggcggcc tgacgctgga caccgaggag
     2101 tacgcgctcg tcacctcccg aagcccgcgg tactatgtaa gcgccgcgta ctgggaccgg
     2161 gacagcctgc tctggagctt tcccgcgatt ctgctcatag acccgccgac cgcgcgggag
     2221 atgcttcaat acgttttcac ccggcagata cgaaacatcg gcgtgcacag ccgctttatc
     2281 gacggaacgc tgctggagcc ggggtttgag ctggacgagc tctgcgcacc ggtcatcgcg
     2341 ctcaaccgct acatcaaaac cacgggagat accgctctgc tgcaaaaaga gtgcgttcgc
     2401 gagggcatcg agcatatcct tgcaagactt cgttccatgc ggcacgcaag cgtcgcgctg
     2461 tacgaaacct ttctgcagcc gacggacgac ctgatcgaac acccgtacct gacttatgac
     2521 aatgttctgg tctggtattc gctgctcgcg ctggcggaga tgttttcgaa cgtctggccg
     2581 cagggacgaa ttaaggaaat ggagcgcgaa gcggaacagg tcaaacgagc cgtctgggaa
     2641 cactgcgtgt tcgaaaagga cggcgaggat atcttcgcct ggtcggtcga tttgaacggc
     2701 aagtggaata tctacgacga gccgccggga agcctgctgc tgctgccgta ctacggtttc
     2761 tgcggcgaaa acgatcccgt ctggcaggcg agcgcaagga cgattcgccg cgcggactat
     2821 ccttactcgt ttgcggactg tcccattgcg gaaatcggct gcccgcacgc gccgtatccc
     2881 tgggtgctga gcatatccaa cagcctgctc tccggcaggc gcgagcaggc gaaacaacat
     2941 cttctgagaa cgcagatgga caacggcgtc gcctgcgaaa gcgtcgatcc ctataccggc
     3001 gagtgccgca cgggggaagc gttcgccacc tgcgcgggat ttctcgccta cgccataaac
     3061 gaagcgttcg gcggcaaaaa agttcaaaac gatacaccat caaacagata gaaggaatgg
     3121 aaccgggaat ggaatggatc aagattgcgc cgtgggatat ctcttgcgag ggcgcagaag
     3181 aggaaacgcg cgattaccgc gagagcgtgt tttcgcaggg gaacggatat ctggggatgc
     3241 gcggctaccg gccggacgaa gcttccccca atcaggcgca ccgcaccacg ttttattcgg
     3301 ggttttacga atatatccgt ccgggcatta ccgatctggt caatcagccg gactgctcgt
     3361 ataccgaaat taggctcagc ggcaaacccg cccgtgaaat gcgtgtcagt gaggcaaaac
     3421 agtcgttttc gatgaagaca ggcgaagcct cctggcagta tatgctggag gacggcgaag
     3481 ggcgcaaaac ccaggttgag tcgttccgct ttgcgagcat ggacaaccgc cgtctcgtcg
     3541 cgctgcgcta tcgaatcacg ccgcttaact acgaaggaac gctggagctg acaaccggtg
     3601 tggacggcgg ggtggagaat ctgccca
//
LOCUS       DAOMGY010000076         3611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_27029_length_3611_cov_2.522778, whole genome shotgun sequence.
ACCESSION   DAOMGY010000076 DAOMGY010000000
VERSION     DAOMGY010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27029_length_3611_cov_2.522778"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1940
                     /locus_tag="PLR57_01905"
     CDS             <1..1940
                     /locus_tag="PLR57_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQQ40239.1"
                     /translation="IIVETLKCIALPVLAIYPLGTMIMCMVITYEGKSQQFEQKLTES
                     EIRFRAVCEQAPVGITVETTEQVLYANSEISNILGMSTEEIEEVSWQSYTHPEDLQKD
                     VELFHQLLAGEIDKYDLVKRYIRKDGTIVWVHMFVSILHRSADDNNEYICIIQDITDE
                     VDQECSLLESERKQREASAFLATLLDAIPDHIFYKSKSGVYLGCNKAFEEASGISRDQ
                     LVGRTDVQVYDEETACFFIEADQAVLNGEEQVRTEEMVTYPNGGIIVTETLKTRFLDD
                     EGEIAGLIGISRDITDRKKKEERIEYLSIHDIMTGLYSRMYFDTELYRQDTIQELPYS
                     VIMGDINALKLTNDLFGHNEGDKLIIQTAELLKSCCKKGIVARIGGDEFAVLLPGVDE
                     EELKEIVNRIYTKLEDQKKNNKEGSVFLSISLGYSTKTSAEHTLSDVLKTAEEQMYRR
                     KLLDHQSIRSTLLTTIKELLFSKSNETLEHAERMTVLAKRLGDKVGLSEADMDALELM
                     ATLHDVGKIGISNHILSKAGSLDDLEWTEIRKHPEIGYRIALTIPELQGIAGYILCHH
                     ERWDGKGYPQGLSGEEIPFISRMISVIDAFDAMTEDRPYKKAITTLEAIEEILRNAGT
                     QFDPAIAKVFVENVLRFEQKL"
     gene            complement(1976..2686)
                     /locus_tag="PLR57_01910"
     CDS             complement(1976..2686)
                     /locus_tag="PLR57_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQQ40240.1"
                     /translation="MDERKTPLFSEIAEASPFIEMPELDWSSVTHDLELSTFNSGMII
                     YQQDTVANYIYLVKKGRVRVDLYSYSGEYKSLFFASTGCLFGEISTIDGLPNRGTAIA
                     TVPSKVYLIPKARFQYEMSHNIQFCNNVALLLARKFRFLTAEIELQSFYNSYYKVGFV
                     LTHLVRSYGTKTDEGYRLNIGFTHQEMASLTGLSRVSVSKILSDFKTRGIIDKRQGNI
                     IIPDVDALYKMLLENVNI"
     gene            complement(2884..>3611)
                     /locus_tag="PLR57_01915"
     CDS             complement(2884..>3611)
                     /locus_tag="PLR57_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HQQ40241.1"
                     /translation="LVVPVLLIIMMFSGANLIVALMTSGFAGVVLALVCKFITFKDVM
                     FVNLETFSVEGIITSGIVNMIDIAVFAFLLMGLIRLLDESGLLHWVIEKLSKMAKSPR
                     TAELWIAIINIILNILTVASTIVIVMEGPIAKKLLVGKYNITPSHSANMLDAVAAGAM
                     CLIPYGFAPLLAVMFSGTTGVSVFQVCLNSFYGYTLLAVMFIAILTGWGRKYIKPEVA
                     KEMIEDENKVLSLKELDEVTLSK"
BASE COUNT          962 a          850 c          992 g          807 t
ORIGIN      
        1 agatcattgt cgaaacactc aaatgcatcg cgctgccggt gctggcaatc tatccgctgg
       61 gtacgatgat aatgtgcatg gtcatcacct acgaaggcaa gagtcagcag tttgagcaga
      121 aattaacgga aagcgaaatc cgttttcgag cggtatgcga gcaggctccg gtggggataa
      181 cggtggaaac caccgagcag gttctttacg ccaactcgga aatttcgaat attcttggaa
      241 tgtcgaccga agaaatcgaa gaagtcagct ggcagtccta cacgcatccg gaggatctgc
      301 agaaggatgt tgagctcttt catcaactgc tggcaggcga aatagacaag tatgaccttg
      361 tgaaaagata cattcgcaag gacggtacca tcgtttgggt tcatatgttc gtctcgattc
      421 tgcaccgcag tgcggacgat aacaacgagt atatctgcat tattcaggat atcacggatg
      481 aagtcgatca ggaatgcagc ctgctggaga gcgaacggaa acagagagaa gcgtccgcgt
      541 ttcttgcaac gctgctggac gccattccgg atcatatctt ctacaagagc aaaagcggag
      601 tgtacctcgg ctgcaacaaa gcgtttgaag aggcatccgg catttcccga gaccagcttg
      661 tcggcagaac agatgtccag gtttacgacg aagagacagc gtgtttcttc atcgaagcgg
      721 atcaggcggt tttgaacggc gaggagcagg tgcgaacgga agaaatggta acctacccga
      781 acggcggaat tatagttacc gaaacgctga aaacgcggtt cctagacgac gaaggcgaaa
      841 tagccggatt gattggaatc agcagagaca ttacggacag gaagaaaaaa gaagagcgga
      901 ttgagtacct gagcattcat gatatcatga cggggcttta cagccgcatg tattttgaca
      961 ccgagttgta ccggcaggat acgattcagg aactgccgta ctcggtcatt atgggcgata
     1021 tcaacgcgct gaaactgacg aacgatctgt ttgggcataa cgaaggtgat aagctcatca
     1081 ttcagactgc cgagctattg aaaagctgct gtaaaaaagg aatcgtggcc cgcattggcg
     1141 gcgacgagtt cgcggttttg ctgccgggag tagacgagga agagctgaaa gagattgtca
     1201 accgaatcta cacaaagctc gaagatcaaa agaagaacaa caaggaaggc agcgtgttct
     1261 tgagcatttc gctggggtat tccacaaaaa ccagcgcaga gcatacgctt tcggacgtac
     1321 tgaagactgc cgaagagcag atgtacagaa gaaagcttct cgatcatcag agcattcgca
     1381 gcacgctttt gactacgatt aaggaactgc tgttctccaa gagcaacgaa acgctggagc
     1441 acgcggaacg catgacggtg ctggcaaaac ggctcgggga caaagttggc ttgagtgagg
     1501 cggatatgga cgcgcttgaa ctgatggcga cgctgcatga cgttggcaaa atcggcatca
     1561 gcaaccatat tctatccaag gcgggctctc tggacgactt ggagtggacg gagattcgca
     1621 agcatccgga aatcggctac cgcatcgcgc tgaccattcc cgaactgcag ggcatcgcgg
     1681 ggtacatcct ctgccaccat gagcgctggg acggcaaggg gtatccgcaa gggctgtccg
     1741 gggaggaaat cccgttcatt tcgcggatga tatccgtcat cgacgcgttc gacgctatga
     1801 cggaagatcg tccctataaa aaggcgatca cgaccctaga ggccatcgaa gagattctgc
     1861 gaaacgccgg aacgcagttc gatccggcca tcgcaaaggt ttttgttgaa aatgtcctgc
     1921 gatttgagca aaaactataa agcttgcttt ttcccgcagg ggagaaaagc acgcgctaga
     1981 tattcacgtt ttccagcagc attttataga gcgcgtccac gtcggggatg atgatatttc
     2041 cctgacgttt atcgataatg ccgcgcgttt tgaagtccga tagtatcttg gagaccgata
     2101 cgcgggacag tccggtcagc gaagccatct cctgatgggt aaagccgatg ttcagccgat
     2161 atccctcgtc ggtcttcgtg ccgtaggaac gcaccagatg tgtaagcacg aacccgactt
     2221 tgtaatagga gttataaaag gactgcagct cgatttccgc ggtcaggaag cggaattttc
     2281 tggccagcag gagtgctacg ttgttgcaga actggatgtt gtggctcatc tcgtactgga
     2341 agcgcgcttt cggtattaag tacacttttg aaggaaccgt agcaattgcg gtgccacggt
     2401 tcggcaggcc gtcgatggtg gagatttcac cgaacaagca gcccgtagaa gcgaagaaaa
     2461 ggcttttgta ctcgccgctg tagctataca aatcgacgcg gacgcgtcct ttcttcacca
     2521 agtagatgta atttgccacc gtatcctgct ggtagatgat catgccgctg ttgaacgtgg
     2581 agagctccaa atcgtgcgta acggaactcc aatccagctc aggcatttcg ataaaaggcg
     2641 acgcttcggc gatttcgctg aacaacggcg ttttcctttc gtccatcgag tgaacctcct
     2701 ctccggtcaa gtgtccacgg tttattgaaa caatattcaa cgttactata tcatttgttg
     2761 ctttacaaga caaataacga tacttaagcc attaaaaacg aatcaaatgc tgaatttcta
     2821 tatgcacaaa cgaacggccg gtggaccgtt cgtctgtgca tgttttttgg aggtattgtc
     2881 cggttatttg ctgagtgtaa cttcgtcgag ctctttgagg ctgagtactt tgttttcgtc
     2941 ctcaatcatc tcttttgcca cttccggctt aatgtacttg cgtccccaac cggtgaggat
     3001 ggcaatgaac atgaccgcaa gcagcgtata gccgtagaag ctgttgaggc agacctggaa
     3061 cacggaaaca ccggtcgtgc cgctgaacat gacggcgagc agcggagcaa acccgtaagg
     3121 aatcaagcac attgcgcccg cggcaaccgc gtccagcatg tttgcgctgt gggaaggcgt
     3181 aatgttgtac ttgccgacga gcagcttctt tgcaatcggc ccttccatta cgatgacgat
     3241 ggtggaagca accgtcagga tgttgaggat gatgttgatt atcgcaatcc agagttccgc
     3301 ggtgcgcggg gacttggcca tcttgctgag cttttcaatg acccagtgca gcaggccgga
     3361 ttcgtccaga agacggatta agcccatcaa caggaacgcg aacaccgcaa tatcgatcat
     3421 gtttacaatg ccgctggtga tgatgccttc gacgctgaac gtttcgaggt tgacgaacat
     3481 aacgtctttg aaggtgatga acttacagac cagtgcgagc acaacgcctg caaagccgga
     3541 cgtcatcaac gcaacgatca ggttggcgcc ggagaacatc atgatgatga gcagaacagg
     3601 aacgacgagc a
//
LOCUS       DAOMGY010000077         3563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_27533_length_3563_cov_2.736602, whole genome shotgun sequence.
ACCESSION   DAOMGY010000077 DAOMGY010000000
VERSION     DAOMGY010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27533_length_3563_cov_2.736602"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..945
                     /locus_tag="PLR57_01920"
     CDS             211..945
                     /locus_tag="PLR57_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HQQ40242.1"
                     /translation="MQAAIIGGTNLEEIPVPYREQPVVTPYGDVIVYRGTLDNGQDVI
                     FLSRHGVLYRADPADINYRANIYALYSLGVTHIIGLSSVGACDYAYKLGTVCLINDFI
                     DFTKSRAHSFDREHRLALHTGMEDVCCPDLNDALEQMILTRGLPYSGRVVYACTEGPR
                     FETAAEVRMFRTLGAQIIGMTLVPEAPLARDLGMNYAALGIIANYCTGMTSYVTDDGI
                     ENVIRGMRESVFELCFDFIKNASNQP"
     gene            complement(1039..1695)
                     /locus_tag="PLR57_01925"
     CDS             complement(1039..1695)
                     /locus_tag="PLR57_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQQ40243.1"
                     /translation="MERLDDLQHNGLMLFQDTDYARFNADALLLLGFLRLNKRDSVVE
                     LGSGTGVVCVLGADVFGCSFTGVEPQQRLVELAQKSADYNHQQIRFVCQTASGAPELL
                     GRGQFSAVVMNPPYFAEGDRSGNPSVALSRHAASGVLEEFLSAAFALLDNGGKLFVIY
                     PAAALCDLFTALRAHRLEPKRMRFVSAEPGENASRVLVEAKKLGKPGLLVEPPALMQN
                     "
     assembly_gap    1929..2028
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2077..2715
                     /locus_tag="PLR57_01930"
     CDS             2077..2715
                     /locus_tag="PLR57_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40244.1"
                     /translation="MITNDLQKRIQHGDREAFRSVYTKYGRDVYLTALHALGSEEAAR
                     GVVKQTFLVLHNELMRASDDVDLAVRIRELSENELLLLEILKTGGGEQAASAVAERAK
                     STARARTYVEPETEPEDETESAIPEDLPPLERTRAYMRADHAPGAAPKREKKSGSSRF
                     GHAISVFLLFVFLLLLLWTLAGVLMGLGFIPKFDLGYGWFNNSVFPLFHLGA"
     gene            2778..>3563
                     /locus_tag="PLR57_01935"
     CDS             2778..>3563
                     /locus_tag="PLR57_01935"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HQQ40245.1"
                     /translation="MIIRFSLDAARRLFTPVDNRFILDFLPDASDVCIRVYLYGLMLC
                     TSGAAAETDLGDALGLSETAVKEAYVYWQNQGLVHIRGEEPLIVEYLDLEPTKEGGVI
                     PGKYASLVSALNSLIAPRQFDFRELVHVYDWIEVYGLDEETVLELVSHCMSIKNRRVT
                     VNYIDSVARTWSEEGIKTREKARAYIEGYELKKHGATLILKQWNKQRKPTQDEMSLYE
                     KWVGEWGFTQEAILASLPKLTVTGSPNFIYLDELLDSLRREGQT"
BASE COUNT          770 a         1033 c          931 g          729 t
ORIGIN      
        1 gaacgctgcc gttttcgtac caggcgatat tttcaaattt gaataaaaac gcaagatcgt
       61 gatcggctct caaacgcatg acggttctcc tcatcgattg cttcgctctc tgtacagaac
      121 ggcggattat tcgctttggc cgttttacgg ctgtttccag tatacacttt ttcggttccg
      181 tatgatatag tcaaaccgag gtgatggaac atgcaggcag cgatcatcgg cggcactaat
      241 ctcgaagaaa ttcccgttcc ctatcgtgaa cagcccgtcg ttacgcccta cggcgacgtg
      301 atcgtttatc gcggcacgct ggataacggt caggacgtga tctttctttc gcgtcacggc
      361 gtactctacc gcgccgaccc ggcggacatc aactaccgcg ccaatatcta cgcgctctac
      421 tcgcttgggg tgacgcatat catagggctc tccagcgtcg gcgcctgcga ctatgcctac
      481 aagctcggca cggtatgcct catcaacgat tttatcgact ttaccaagag ccgcgcgcat
      541 tcctttgacc gcgaacaccg cctggcgctg cataccggca tggaggacgt ttgctgtccc
      601 gacctgaacg acgcgctgga gcagatgatt ctcacgcgcg gcctgcccta ctccggcagg
      661 gtggtctatg cctgcacgga aggcccacgc tttgaaaccg cggcggaagt gcgtatgttc
      721 cgtacactcg gcgcgcagat tatcggcatg acgctggtgc cggaagcgcc gcttgcgcgc
      781 gacttgggca tgaactacgc ggcgctcggc atcattgcca actactgcac gggaatgacc
      841 tcctacgtta ccgacgacgg catagaaaac gtaatccgcg gcatgcgcga gagcgtgttc
      901 gaactctgct tcgactttat taagaacgca agcaaccagc cgtaatccac actgtttccc
      961 gattatgact tctcccaaaa caaaaacagc ttttccgaat cgaagagctg tttttcaatt
     1021 gctactctgt gtaacgaatc agttttgcat cagcgccggc ggttccacta agaggccggg
     1081 tttgccgagc tttttggcct ccacgagcac gcgagaagcg ttttctcccg gctcggcgga
     1141 gacaaaccgc atgcgtttgg gttcgagccg gtgtgcgcga agggcggtga ataaatcgca
     1201 gagcgcggcg gcgggataga tgacgaagag ctttccgccg ttgtcgagca gcgcgaacgc
     1261 cgccgaaagg aactcttcca gcacgccgga agccgcatgg cgcgagagcg caacggaagg
     1321 attgccgctg cggtcgccct cggcgaagta cggcgggttc atcacgaccg cgctgaactg
     1381 cccgcgcccc aagagctccg gcgcgcccga cgcggtctgg cagacaaagc gaatctgctg
     1441 atggttgtaa tccgcgctct tctgcgcaag ctctacaaga cgctgctgcg gctccacacc
     1501 ggtaaaggag cagccgaaca cgtccgcacc caacacgcag actactcctg tgccgctgcc
     1561 gagttcgaca acgctgtccc gcttgttcag ccgcaaaaag ccgagcagca acagcgcgtc
     1621 ggcattgaag cgcgcgtaat ccgtgtcctg aaacagcatc aatccgttgt gctgcaggtc
     1681 gtccaatcgt tccataaaaa gaagtatagc atattttttg ctccttcccg catgaataag
     1741 ccgcatcccc gccgcgcttt tcttttattg ctcaacatgc cgccgaggat ggcattggaa
     1801 acgtattccg cgcgtttgaa gcgctctaag ccgcatcccc gccgcgcttt tcttttattg
     1861 ctcaacatgc tacaattatg aaaaccgctc cccgaaaaaa agcagagaag gaagcaatgc
     1921 catgatcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa ttatgaaaac
     2041 cgctccccga aaaaaagcag agaaggaagc aatgccatga tcacaaacga tctgcaaaag
     2101 cgcattcaac acggcgaccg cgaagcgttt cgttcggtct acaccaagta cggccgcgat
     2161 gtttacctca ccgcactgca cgcgctgggc agcgaggaag ccgcgcgcgg cgtggtcaag
     2221 caaacgttcc tcgtacttca taacgaactg atgcgcgcct cggacgacgt tgatctcgcc
     2281 gtccgcatcc gcgagctttc ggaaaacgag ctgcttttgc tggagattct caaaaccggc
     2341 ggcggagagc aagccgcttc cgctgtcgcc gaacgcgcca aaagcaccgc gcgggcacgg
     2401 acatatgttg aaccggaaac cgagcccgaa gatgaaacgg aatccgccat tccggaagat
     2461 ttgccgccgc tggagcgcac gcgcgcctac atgcgcgcgg atcacgcgcc gggcgctgcg
     2521 cccaagcgcg aaaagaaaag cggttcttcc cgctttggcc acgcgatcag cgtgttcctg
     2581 ctctttgtat ttctccttct gctgctctgg acgctcgcag gcgtgctgat ggggctcggg
     2641 ttcatcccca agttcgatct tggctacggc tggttcaaca acagcgtgtt cccgctcttc
     2701 catctgggcg cgtaagtaaa agccgttacg gctgtgaaac cctcatgacg aatcgttaaa
     2761 aggaagcctc aattggcatg atcatccgtt tttctctgga cgcggcgcgg cgcttgttca
     2821 cgcccgtaga caaccgattt atcctcgatt ttctgccgga tgcaagcgat gtctgcattc
     2881 gggtgtatct ctatggcctg atgctctgca cgtcgggcgc ggctgccgaa acggatctcg
     2941 gcgacgcgct gggcttatcc gaaaccgcgg tcaaggaagc gtatgtctac tggcagaatc
     3001 aggggcttgt gcatattcgc ggcgaagagc cgcttatcgt tgaatacctc gacctcgaac
     3061 ccaccaagga aggcggcgtc atccccggta aatatgcctc cctcgtttcc gcgctgaatt
     3121 cgctgattgc gccgcgccag ttcgattttc gcgaactcgt gcatgtttac gactggattg
     3181 aggtatacgg cctcgacgag gaaactgtgc tcgaactcgt ttcgcactgc atgagcatca
     3241 agaaccgccg cgtaaccgtg aactatatcg actccgtcgc ccgcacctgg tctgaggaag
     3301 gcatcaagac ccgcgaaaaa gcgcgcgcct atattgaagg ctacgagctg aagaagcacg
     3361 gcgcaaccct gattttgaaa caatggaata aacagcgcaa acccacgcag gacgagatgt
     3421 ctctctacga aaagtgggtc ggcgaatggg gctttacgca ggaagcaatt ctggcttcgc
     3481 tccccaagct cacggtaacc ggctcgccga actttatcta cctggacgag cttttagatt
     3541 ccctacgccg cgaaggccag acc
//
LOCUS       DAOMGY010000078         3551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_27677_length_3551_cov_2.974828, whole genome shotgun sequence.
ACCESSION   DAOMGY010000078 DAOMGY010000000
VERSION     DAOMGY010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27677_length_3551_cov_2.974828"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLR57_01940"
     CDS             complement(<1..762)
                     /locus_tag="PLR57_01940"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HQQ40246.1"
                     /translation="MKSVLYRFICGIAVGIGGVLPGVSGGILAISLGMYEKMMLAIGD
                     FFHNIKANFKYLLPIVLGGGIGILLTSNVLSILIARYEAPLLALFSGLVLGSVPGLYS
                     EVRKSGPIKPKHIIAALLGLGIVLLFALGENTAVASGKMAELTIPVSLLAGAVLAFGV
                     VIPGVSSSFILIYMGLYSAVIGVISGVLDLKALATGGVSGAFNRFLEILVPLIFLSIG
                     FGLCALIIIKFVNRMMKRHHTSSYAAIIGFVIGSVA"
     gene            1166..2668
                     /gene="malQ"
                     /locus_tag="PLR57_01945"
     CDS             1166..2668
                     /gene="malQ"
                     /locus_tag="PLR57_01945"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HQQ40247.1"
                     /translation="MKLGVHMKRSSGILLHISSLPSPYGIGSLGREAYAFVDQLERAR
                     QSYWQVLPLGPTGYGDSPYQTFSAFAGNPYFIDLETLVNEGLLDRSALDAIDWGSPAR
                     QVDYGKIYFNRFTVLKSAFARAMQDSEKTRALDDFSQKNADWLEDYALFMSLKDENGG
                     VAWNKWSQETRMREQSALDSARSRLGESIRFYKFIQCLFFEQWVKLKAYANEHGIRIV
                     GDLPIYVPLDSADVWSAPEEFQLDEERRPRCVAGVPPDYFSEDGQLWGNPIYDWDYMK
                     QTGYAWWIRRMRSAATLFDCLRIDHFRGLSSYWSVSADAETARGGVWVEGPGIGFVDA
                     IKAACPQMEVIAEDLGFLTDEVRSLLKQSGFPGMKVLQFAFDAREPSNYLPHTYDRHC
                     VCYAGTHDNTTTAGWFSEVDPLAVGYAMEYLGLNEREGYVWGILRGGMGSVSDLFVAQ
                     MQDYLGLDATGRMNIPGTLGGNNWRWRLLPGEFTDELIEKIAAMTTIYGR"
     gene            complement(2735..>3551)
                     /locus_tag="PLR57_01950"
     CDS             complement(2735..>3551)
                     /locus_tag="PLR57_01950"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQQ40248.1"
                     /translation="ALQYIEQHLLEEADSAQAARHVGLSRFYLERTFAALTGMSVSEY
                     IRARRLSQAANELIAGDAKIIDLALKYGYDTPESFTKAFSRFHGVTPTNARRFSTLLK
                     CQNPLAISIKLEGFTRMNYKIEQMDAFTVFGAERSFHMDNSKEEIPKFWDEFFRLGLA
                     EKVCPVFGICFDADASGNFPYMIGEKLEPGMVVSEGLVTREIPAHTWARFACVGAMPG
                     AIQTATQQIYSEWLPTNGVYEVAQYVEIEMYSEGDPSKDDYYSEIWIPIRKKA"
BASE COUNT          780 a         1007 c          986 g          778 t
ORIGIN      
        1 cgcaaccgat cctatcacaa agccgataat cgcggcgtag gacgacgtat ggtgccgctt
       61 catcatgcgg ttgacgaatt tgataataat cagcgcgcaa agcccgaagc cgattgaaag
      121 aaagatcagc ggcacgagga tttccagaaa gcggttgaac gcgccggaaa cgccgccggt
      181 agcgagcgct tttaagtcga gtacgccgga gatcacgccg atcaccgcgc tgtagagacc
      241 catatagatt aggataaacg aggagctaac gccgggaata acgaccccga acgctagcac
      301 ggcgcctgcg agcagagaaa cgggaatggt cagctccgcc atcttgccgc tggcgacagc
      361 ggtgttttcg ccgagagcga acagcagaac gatccccagc cccagcagag ccgcaatgat
      421 atgcttcggt ttgatcgggc cgctctttct tacttccgaa tacaaccccg gcacgctgcc
      481 gagcaccaga ccggagaaga gcgccagcag cggcgcttcg tatcgcgcaa tcaggatgct
      541 gagcacattg cttgtcaaca ggattccaat ccctccgccg agcacgatgg gcagcagata
      601 tttgaagttc gcttttatgt tatgaaagaa gtctccgatg gcaagcatca tcttttcata
      661 catgccgagg ctgatggcaa ggattccgcc ggagaccccc ggcagtacgc cgcctatgcc
      721 aacagctatt ccgcagataa atcgatagag cacagatttc atttcgttcc ctcaaaaagg
      781 cgcacctgcg cggcaatgta cgcgcctgag cgcgccgggg ctgcgtttgg cttcagcgac
      841 cgaatctttc gttttttatg acaagcgctt tctacgacaa gtcaaagcga aaattaaacg
      901 gtcgctgcta aaattgtgtt gcaaaaaaca accgcttcta ttatatcagt ccgggcaagc
      961 gcctaagtga aatttcagca aacttttgca tgattgcagc atatttattg atatggttct
     1021 agattttcgc tttgccagtc ggttttgcag cttccgtctg ctccgcagat tcgcgctgcg
     1081 caacgcaata accgctttcc ataatcgttc ttttgctgag aaacaacttc atgtataata
     1141 ctgttgttgc tcaacgatga atttaatgaa acttggtgtg catatgaagc gttcttccgg
     1201 catattgctg catatttcat ctcttccatc cccgtacggc atcggttcgc tcgggcggga
     1261 agcctatgcg ttcgtcgatc agctcgagcg cgcccgccag agctactggc aggtgctgcc
     1321 gctcgggcct acagggtacg gcgactcgcc gtatcaaacc ttttccgcgt ttgcaggcaa
     1381 cccgtatttt attgaccttg aaacgctggt gaacgaaggc ctgctcgacc gttccgcgct
     1441 ggacgccatc gactggggtt cccccgcgcg gcaggtggat tacggtaaaa tctacttcaa
     1501 ccggtttaca gtgctgaaaa gtgctttcgc acgcgcgatg caggattctg aaaagacgcg
     1561 cgcgctggac gatttttcgc aaaagaatgc ggactggctg gaagactacg cgctgtttat
     1621 gtccctcaag gacgaaaacg gcggcgttgc ctggaacaaa tggtcgcaag aaacgcgcat
     1681 gcgcgaacaa tcggctctcg attccgcgcg gagccgtctg ggtgaaagta tccgttttta
     1741 taagttcatt caatgcctgt tctttgaaca gtgggtaaag ctgaaggcct acgccaatga
     1801 acacggtatc agaattgtcg gcgatctgcc catctacgtt ccgctggaca gcgcggacgt
     1861 ctggagcgcg cccgaggagt ttcagctgga cgaagagcgc aggccgcgct gcgttgcagg
     1921 cgttccgccg gactacttca gcgaggacgg tcagctttgg ggcaacccga tctacgactg
     1981 ggactatatg aagcaaacgg gctacgcgtg gtggattcgg cgcatgcgct cggcggcgac
     2041 gctgttcgac tgcctcagaa tcgaccattt ccgcgggctc tcctcctact ggagcgtgag
     2101 cgcggacgcg gaaaccgcgc gcggcggcgt ttgggtggaa ggaccgggaa tcggttttgt
     2161 agacgccatc aaggccgcct gcccgcagat ggaagtgatc gccgaggact tgggcttttt
     2221 gaccgacgaa gtgcgcagcc tcttaaagca gagcggcttt ccgggcatga aggtgctcca
     2281 gttcgcgttc gatgcgcgcg aaccgagcaa ctacctgccg catacctacg accgccactg
     2341 cgtctgctac gcgggcacgc acgacaacac caccacggcg ggctggtttt ccgaagtgga
     2401 tcccctcgcg gtcggctacg cgatggaata ccttggtctg aacgagcgcg aaggctacgt
     2461 ctggggcata ctccgcggcg gaatggggtc ggtttccgac ctgtttgtgg cgcagatgca
     2521 ggactatctc ggccttgacg caacggggcg catgaacatc cccggcacgc tcggcggcaa
     2581 caactggcgc tggcggctgc tccccggcga gtttaccgac gaattaatcg aaaagatcgc
     2641 cgcgatgacg acgatctacg gacgataaga agtttctttg tgaaaaaaac ggaacctcgc
     2701 ggttccgttt tttgtatgtg ctgcttaaaa tcccttacgc tttcttgcgg atcgggatcc
     2761 agatttcgct gtagtagtcg tctttcgaag gatcgccttc cgaatacatc tcaatttcca
     2821 catactgcgc aacctcgtac acgccgttgg tcggcagcca ttcggaataa atctgctggg
     2881 tagcggtttg aattgctccc ggcatagcgc caacgcaggc aaagcgcgcc catgtatgcg
     2941 cagggatttc gcgcgtaacg agcccttccg aaaccaccat gcccggttcg agcttttcgc
     3001 cgatcatata gggaaagttg ccgcttgcgt ccgcgtcgaa gcagatgcca aacaccgggc
     3061 aaaccttttc cgccagcccc aaacgaaaga actcgtccca gaacttcgga atctcttcct
     3121 tgctgttgtc catgtgaaac gagcgctccg cgccgaatac cgtaaaagca tccatctgtt
     3181 caatcttgta attcattctc gtaaatccct ccagttttat tgaaatcgca aggggatttt
     3241 ggcatttgag cagtgtgctg aaccgccgcg cgttggtcgg cgtgaccccg tggaagcggg
     3301 agaacgcctt ggtaaagctc tccggcgtat cgtagccgta cttcagcgcg aggtcgatta
     3361 tctttgcgtc gcccgcgatc agctcgtttg ccgcctgcga gagcctgcgc gcgcggatgt
     3421 actcgctcac gctcatgccg gtcagcgccg cgaacgtccg ctccagatag aagcgcgaga
     3481 ggcccacgtg ccgcgccgcc tgcgcgctgt ccgcttcctc cagcaggtgc tgctcaatat
     3541 actgcagcgc g
//
LOCUS       DAOMGY010000079         3541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_27800_length_3541_cov_2.350545, whole genome shotgun sequence.
ACCESSION   DAOMGY010000079 DAOMGY010000000
VERSION     DAOMGY010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27800_length_3541_cov_2.350545"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLR57_01955"
     CDS             <1..804
                     /locus_tag="PLR57_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40249.1"
                     /translation="GIGDYSAFVAGGITFAADTSWSKPLLAEVGDYKKLPPLGTSVWY
                     QRFLLICDKLMTAMGTCGLPFTRGFFSPMDLAGALRGEGLYFDFYDDPDELRGLLDYC
                     ADATIRFAEDIYALAERRLGSTKYGTFYLRDKINMSEDISCMISGELYRTFAAPATQK
                     VIDHFGVGHMHTHSRAMYLVKEICALKNVANLWLATDPNQPRPIEHIKELCADSNGVC
                     IAIDCENTDEIERNLDELKHGNFSICMPVASYKEAQAAAARFDCYRSRG"
     gene            807..3161
                     /locus_tag="PLR57_01960"
     CDS             807..3161
                     /locus_tag="PLR57_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQQ40250.1"
                     /translation="MKRKAALCLLTAAIAAVSFALPSSVARAEGNGEQSDSGSASAGT
                     VKVWFGANEEENAVLEEIAERFEQETGISVEVVARRAIFDAPTDLVNYAQLEEAPDIV
                     YIQAPDIGTLVRSGFLEPLDFSAETKSRFTDVAFSAFTLNGLCYGVGYNNSTSGLLYN
                     KALISKEELPKTWDEFFSTAERLTIPDGSGGYSQFGTLLNARNMWFVYPIIKEYGGYY
                     YGQLADGTYNPYDVGLDNAGMLSYVEKIKELQAKGLVLGTRVASESNIVSAFGEGKVA
                     MMLYGLWSADYLKGLGVDYGIAPLPAQSNGEPSKPLTTVEGFVVNHYSRHTDAAKQFL
                     NFILEDGNQQLLVEAGNGYALKTGARNPTNLSVIASDYIQSDEILKNLSAIGENSEPF
                     PNIPEGTIWYNYTTTAFGTIFYGDSSGKAVDAEAKLHELANAVRSDVARMNEQTERVD
                     IPWWAFAVSGVLFVLGVSLAFFRRQKRRPLEVHTRYPKGSALIGWGLMTPLICLLALF
                     YIYPILHNIALSLTNYSSIHLTDYEYVGVANYKNILTEGVQGLLSMLLWTVLFAISVV
                     TLAFILGTFIATLLERVGVRVGRAYRVVFILPWVIPTVITLLMWQGLLETDGGLVNQL
                     LGLVGIPGVPWLSDPVWARVSSILVMVWFAFPYFMVVAFGILKSIPRDYFEAARIDGA
                     SNAHIFRSITLPIVFKAMKPTLIMSLIMQFNQFGIYLLTQGGPASDKLGAPGATDLLI
                     TYIFNTAFNTGSYAKAAAYSVILFLFIGTFSLVAMNRGSRKEAD"
     gene            3163..>3541
                     /locus_tag="PLR57_01965"
     CDS             3163..>3541
                     /locus_tag="PLR57_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40251.1"
                     /translation="MKRRLSRFLIQAFLLVLAICVLVPFVYIVLISFGKHVSGTSTVV
                     PKAFTLENYAKLFTQTGFLRWMGNSLFVALGTTLIAVALVSVSVYVFSRMRFRGRMQL
                     FNAVLLIQIFPLTLSMVSLFHIFV"
BASE COUNT          713 a         1049 c          999 g          780 t
ORIGIN      
        1 ggcattggcg actacagcgc gtttgtcgct ggcggtatta cgtttgcggc ggatacgagc
       61 tggagcaagc cgttacttgc ggaggtcggc gactataaaa agctcccgcc gctcggtaca
      121 tccgtttggt atcagcgttt cctgctgatt tgcgacaagc tcatgacagc gatgggaacc
      181 tgcggtctgc cctttacgcg cgggttcttt tcgccgatgg atctcgcggg cgctctgcgc
      241 ggcgaagggc tctacttcga cttttacgac gatccggacg aactgcgcgg ccttctcgac
      301 tactgcgcgg acgcgaccat ccgctttgcc gaggatatct acgcgctggc ggaacgccgt
      361 cttggcagca cgaaatatgg aacgttctat ctgcgcgaca agatcaacat gagcgaagac
      421 atctcctgca tgatctccgg cgagctctac cggacgtttg ccgcgcccgc gacgcaaaag
      481 gtcatcgacc attttggcgt ggggcacatg catacacatt cgcgcgccat gtatctggtc
      541 aaggaaattt gcgcactcaa gaacgttgcc aacctctggc tggcgaccga cccgaaccaa
      601 ccgcgcccca ttgaacatat caaagaactc tgcgcggact ccaacggcgt ctgcatcgcc
      661 atcgactgcg aaaacacgga cgaaatcgag cgcaatctcg acgaactcaa gcacggcaac
      721 ttctccatct gcatgcctgt tgcttcctat aaagaagcgc aggctgccgc cgcacgattt
      781 gattgttacc gttcgagggg ataagtatga aacgcaaagc cgcgctctgc ctgctgaccg
      841 ccgccatagc cgcggtttcg ttcgcgctgc cttcttccgt ggcgcgcgcg gagggcaatg
      901 gcgagcagag tgattccggc agcgcttccg ctggaaccgt caaggtatgg tttggcgcaa
      961 acgaggaaga aaacgcggtt ctagaggaga ttgccgaacg ctttgagcag gagacgggca
     1021 tttccgtcga ggtcgtcgcc cggcgggcca tcttcgacgc accgaccgac cttgtcaact
     1081 atgcgcagct cgaagaagcg ccggacatcg tttatattca ggcgccggat atcgggacgc
     1141 tcgtgcgctc cggctttctg gagccgctcg atttttccgc ggaaacaaag agccggttta
     1201 cggacgtcgc gttcagcgcg tttacgctga atggtctctg ctacggcgtt ggctacaaca
     1261 acagcacttc agggctttta tacaacaaag cgctgatttc caaggaagaa cttcccaaaa
     1321 catgggacga gttcttttcc accgccgaac ggttgaccat tccggatggg tccggcgggt
     1381 attcgcagtt cgggactctt ctcaacgcac gcaacatgtg gtttgtgtac cccatcatca
     1441 aggaatacgg cgggtactat tacggtcagc ttgcggacgg aacctataac ccttacgacg
     1501 tcgggctcga caacgcgggc atgctcagct atgttgaaaa aataaaggaa ctgcaagcta
     1561 aagggctcgt tctcggcacc cgcgtggcaa gcgaaagcaa catcgtcagc gcttttggcg
     1621 aaggcaaagt agccatgatg ctctacgggc tttggtcggc ggactatctc aaagggctcg
     1681 gcgtagacta cggcatcgct cccctgcccg cgcagagcaa cggagaacct tccaagccgc
     1741 tcaccacggt ggaagggttc gtcgtcaacc attattcgcg ccatacggat gcggcaaagc
     1801 agtttttgaa cttcatactc gaggacggaa accagcagtt gctcgtggaa gcgggcaacg
     1861 gatacgcgct caagaccggc gcgcggaacc ccacgaacct gtccgtcatc gccagcgact
     1921 acatccagag cgacgaaatc ctcaaaaacc tcagcgccat cggggaaaac agcgaaccgt
     1981 tcccgaatat ccccgaaggc acgatctggt acaactacac gacaacggcg ttcggcacga
     2041 tcttttacgg cgacagcagc ggaaaagctg tggacgcgga agccaagctg cacgagctgg
     2101 caaacgcggt gcgttcggac gttgcacgca tgaacgaaca gacggaacgg gtggatatcc
     2161 cctggtgggc gttcgccgtc tctggagtac tgtttgtgct gggcgtttcg ctggcattct
     2221 tccgcaggca gaagcgccgc ccgctcgagg ttcatacgcg ctacccgaag ggctccgcgc
     2281 tgatcggctg ggggctgatg acgccgctga tctgcctgct tgcgctgttc tatatttacc
     2341 cgattctcca taacatcgcg ctttcgctga cgaattacag cagcatccat ctgaccgatt
     2401 acgaatacgt aggcgtagca aactacaaaa acattctgac cgaaggcgtg caggggctgc
     2461 tctccatgct gctctggacg gtgctgtttg ccatttcagt cgtgacactc gcgttcatcc
     2521 tcggaacgtt catcgcaacg cttttggagc gcgtcggcgt acgcgtaggc cgcgcgtacc
     2581 gggtggtgtt catcctgccg tgggtcatcc cgaccgtgat cacgctgttg atgtggcagg
     2641 gactgttgga aaccgacggc gggcttgtca atcaactgct gggtcttgtc ggcattcccg
     2701 gcgtcccgtg gctcagcgac cccgtctggg cgcgggtgag ttccatcctc gtaatggtgt
     2761 ggtttgcgtt tccgtacttc atggtggtgg cgttcggcat cctcaagtcc atcccgcggg
     2821 attacttcga ggccgcgcgc atcgacggcg caagcaacgc gcatatcttc cgctcgatta
     2881 cactgcccat cgtgttcaaa gccatgaaac cgacgttgat tatgtcgctg attatgcagt
     2941 tcaaccagtt cggcatctac ctgctcacgc agggcggccc cgcttcggac aagctcggcg
     3001 cgccgggggc aaccgacctg ctgattacct acatcttcaa caccgcgttc aacaccggca
     3061 gctatgccaa agccgcggcc tactcggtga ttctgttcct ctttatcggc acgttctcgc
     3121 tcgttgccat gaaccgcggc agccgaaagg aggcggattg acatgaagcg caggctttca
     3181 cgttttctga ttcaggcgtt cctgcttgtg ctcgccatct gcgtgctcgt tccgttcgtc
     3241 tacatcgtgc tcatctcgtt cggcaagcac gtttcgggca ccagcaccgt cgtgcccaag
     3301 gcgttcacgc tggaaaacta cgcaaagctc tttacgcaga ccggttttct gcgctggatg
     3361 ggcaactcgc tgttcgtagc gctcggcaca actctgatcg ccgttgcgct cgtctccgtt
     3421 tccgtgtacg tgttctcgcg catgcggttt cgcgggcgga tgcagctctt taacgccgta
     3481 ctgctgattc agattttccc gctgacgctt tcgatggtct cgctgttcca tatctttgtg
     3541 c
//
LOCUS       DAOMGY010000080         3498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_28311_length_3498_cov_3.110369, whole genome shotgun sequence.
ACCESSION   DAOMGY010000080 DAOMGY010000000
VERSION     DAOMGY010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28311_length_3498_cov_3.110369"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PLR57_01970"
     CDS             complement(<1..1143)
                     /locus_tag="PLR57_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ40252.1"
                     /translation="MQRKTVFIRGALCALPDGASECTIAVWDGKITSIGRFDPPDGAE
                     ILEANGLVALPGAVDSHVHFHMPTADGGYNADDFSTGSTCAICGGTTTVVDFASPVEG
                     KSWTEGVRRRRAEADGKIFCDYGLHMEVTGAFEQDITRLGELADAGVKVLKIYTTYGA
                     DQYPRAKLPALFREAKRLNLRILAHCEDDEIIQETKRRMLAAGKTSAALHAESRPAAA
                     EVAAVRELIELAEQTGTELIIAHVSTGEAGELVAKARKRGVAVYAETCPHYLLLTEEL
                     YADKEPQRTIMTPPLRTASDNALLWELLASGDIAMASTDHCPFLLEQKLAEPTCFGAV
                     PGVGGCENLVSLLFSEGYQKGRLSLPQLAERLSGEAARLYGLSPCKG"
     gene            1510..>3498
                     /locus_tag="PLR57_01975"
     CDS             1510..>3498
                     /locus_tag="PLR57_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HQQ40253.1"
                     /translation="MPLYGEKQFHIINHSAPRIDGIDKVTGRARYAADVSYPFMAYGA
                     MLRLENVPSAQIRSIDASRALALPGVLAVVTAADMPKTKSWASYRYLTDRVRYVGDVI
                     ALIAAETRELAEEAKKLIRVEYDVLPAVFDVREAQQPGAHTVLEDYPDNRFTPSRYTL
                     KKGDPEAGFAEADFIIEREYETQYVEHAYIEPEAAVAIENSTNGEMTVHASAQNPFFT
                     RRYVADILQIPLNRVRLIQETLGGTFGGKEEGAGLLAARCAYLCKLLHRPVKMVFTRE
                     ESLLESSKRHPFQFTYKAGVKRDGRIVAWKATQIDNSGAYNNQTQFMNWRATAHCAGA
                     YSIPNIEAETYGVFTNNVHSGAFRGYSSPELLFAQEQFIEEIGEELGLSPLEMRRINC
                     LKNGSTVATGAEVEHVILPELIDFTAKQTGYERKHAENRTQHSVLRRGIGMAISHRGC
                     GYGAESPDAAGAFLIVNEDGSVLLHSGLAENGQGLKTVYCQIAAEALGVPFESIQFYG
                     TDTHCIPDCGMTVASRGTVMGAQPVRRAGEKLNRILRENAVGLDLFTAGKGGAPRDRK
                     ADDIVLLDSFFFDPDYPDYRVSLAEVAGTALWRGMCLSAYEWFTPPPCLQDHATQQGK
                     AFPTYSYACVVAEVEVDTSTGEVSVLRVTSSHDTGTAIN"
BASE COUNT          680 a         1099 c          987 g          732 t
ORIGIN      
        1 gcctttgcag ggcgaaagcc cgtagagccg cgccgcctcg ccgctaagcc gttctgccag
       61 ttgcggcaag ctcagcctgc ccttttggta cccttccgaa aacaagagtg aaacaaggtt
      121 ttcacacccg cctacgcccg gaaccgcgcc aaaacacgtc ggttccgcga gcttctgctc
      181 cagcaaaaac gggcagtggt cggtggaagc catcgcgata tcgccgctgg caagcagttc
      241 ccagagaaga gcgttgtcgc ttgccgtccg cagcggcggc gtcatgatgg tccgctgcgg
      301 ctctttgtcc gcgtacagtt cttccgtcag cagcaggtaa tgcgggcagg tttccgcata
      361 gaccgcaacg ccgcgtttcc gcgctttggc aaccagctct cccgcttcgc ccgtggagac
      421 gtgcgcaatg ataagttccg ttcctgtctg ttcagcaagt tcgatcaact cccgaaccgc
      481 ggcaacctcc gccgccgcag gcctgctctc cgcgtggagc gctgcgctgg ttttccccgc
      541 tgcaagcatt cgtcttttgg tctcttgtat gatttcgtca tcctcgcagt gtgccagaat
      601 ccgcagattc agccgctttg cctcgcgaaa cagcgcgggc agttttgcgc gggggtattg
      661 atccgcgcca taggtcgtgt agattttcag tacctttacg cccgcgtcgg ccagctcgcc
      721 cagccgcgtg atatcctgct caaacgcgcc ggtgacctcc atatgcaggc cgtaatcgca
      781 aaagatcttt ccgtccgctt ccgccctgcg gcggcgaacg ccctccgtcc agctttttcc
      841 ctcgacgggg ctggcaaaat cgacaaccgt tgtcgttcct ccgcagattg cgcaggttga
      901 gccggttgaa aagtcatccg cgttataacc gccgtccgcc gtcggcatat ggaagtgtac
      961 atggctgtcc accgcgcccg gcaaggcaac aaggccgttt gcctctaaaa tctccgcgcc
     1021 gtccggcgga tcgaaccgcc cgatggaggt gatttttccg tcccagacgg cgattgtaca
     1081 ctcgcttgcc ccgtccggca gcgcacagag cgccccgcgg ataaaaacag tttttctttg
     1141 catggttgcg cctccagaac gcatgttgac cggcagtgct gcttgttccc cgtatgtctt
     1201 tcaaaaagta ttatactgta ttttacattt ggttttacag ttggacataa ttgcaaaaca
     1261 tacatgcaca gatgtgcgta taccccgaaa acgaccgtat tcggggcttt ttcgattgct
     1321 ttgatttttt cgtttccata taatcttcat atagtatatt agatattcat tgctggtatc
     1381 tgttgattcc tctgcatcat ctgttcgctc cggcttttgg cgcatgatct gaacacggtt
     1441 tcccgcgccg gccgcccgca ccccgttcct gttctccgag tacgacgcaa cgttgaggtg
     1501 aaccagtcta tgccgctgta cggtgaaaaa cagtttcata tcatcaacca ttccgcgcct
     1561 cgcatcgacg ggatcgataa ggtcaccggg cgcgcacgat atgccgcgga cgttagctat
     1621 ccgttcatgg cgtacggcgc aatgctgcgg ctggaaaacg tgcccagcgc gcaaattcgc
     1681 tccatcgacg cttcccgtgc gctcgcgctg cccggcgtac tcgcggtggt caccgctgcc
     1741 gatatgccca agacgaagag ctgggcgagc taccgctacc tcaccgaccg cgtccgttat
     1801 gttggcgacg tgatcgcgct gatcgcggcg gaaacgcgcg agctggccga ggaagcgaaa
     1861 aaactgattc gcgtggagta cgacgtcctt cccgctgtgt tcgacgtgcg cgaggcgcaa
     1921 cagcccggcg cgcacacggt tctggaagac tatcccgaca accgctttac cccctcgcgc
     1981 tatacgctga aaaagggcga cccggaagcg ggctttgccg aagcggattt cattattgag
     2041 cgcgaatacg aaacgcagta tgtcgagcac gcgtatatcg agccggaagc tgcggtcgcc
     2101 atcgaaaact cgaccaacgg cgagatgacc gtccatgcct ccgcgcagaa cccgttcttt
     2161 acgcgtcggt acgttgcgga tatcctccag attccgctca accgcgtccg tctgattcag
     2221 gaaacgctcg gcggcacgtt cggcggcaag gaagaaggcg cgggactgct cgcggcgcgc
     2281 tgcgcgtatc tctgcaaact gctgcaccgc ccggtcaaga tggtcttcac gcgcgaggag
     2341 tctcttctcg aaagcagtaa gcggcacccg tttcagttta cttacaaggc gggcgtaaaa
     2401 agagacggcc gcatcgttgc ctggaaagcg acgcaaatcg acaattccgg cgcttacaac
     2461 aatcaaacgc agttcatgaa ctggcgcgcc accgcccatt gcgcgggcgc ctacagtatc
     2521 cccaatatcg aagccgaaac ctacggcgta tttacgaaca atgtccattc cggcgcgttt
     2581 cgcggctact ccagcccgga actcttgttt gcgcaggagc agttcatcga ggagatcggc
     2641 gaggagctcg gcttatcccc gctggagatg cgccgtatca actgcctcaa aaacggctcg
     2701 accgtcgcca caggcgcgga ggtggagcac gtgattctgc cggaactcat cgattttacc
     2761 gcaaagcaga ccggctatga gcgaaagcac gccgaaaacc gcacgcagca cagcgttctc
     2821 cggcgcggta tcggcatggc aatctcgcat cgcggctgcg gctacggcgc ggaatcgccg
     2881 gacgcggcgg gcgcgttttt aatcgtcaac gaggacggtt ccgttctgct gcattccggc
     2941 ctcgccgaaa acgggcaggg gcttaagacc gtgtactgtc agatcgcggc ggaagcgctg
     3001 ggcgttccgt ttgagagcat tcagttttac ggcacggata cgcactgcat cccggactgc
     3061 ggcatgaccg ttgcttcgcg cggaacggtg atgggcgcgc agcccgtccg tcgcgcgggc
     3121 gaaaagctca accgcatcct gcgggagaat gccgtcggcc tcgacctctt caccgccgga
     3181 aagggcggcg caccgcgcga ccgcaaggcg gacgatatcg tgcttctgga cagtttcttt
     3241 ttcgacccag attacccgga ctaccgcgtt tcgcttgcgg aagtggcggg tacggcgctc
     3301 tggcggggca tgtgcctctc cgcctacgag tggttcacgc ccccgccctg cctgcaggat
     3361 cacgcgaccc agcagggcaa ggcttttccc acctactcct acgcctgcgt cgttgccgaa
     3421 gttgaagtcg atacctccac gggagaggtg agcgtgctcc gcgtgaccag cagtcacgac
     3481 acgggcaccg ccatcaac
//
LOCUS       DAOMGY010000081         3490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_28408_length_3490_cov_3.169141, whole genome shotgun sequence.
ACCESSION   DAOMGY010000081 DAOMGY010000000
VERSION     DAOMGY010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28408_length_3490_cov_3.169141"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /gene="gnd"
                     /locus_tag="PLR57_01980"
     CDS             <1..1046
                     /gene="gnd"
                     /locus_tag="PLR57_01980"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HQQ40254.1"
                     /translation="RGPSIMPGGSPAAWPHVKPIFQAISAKTEKGEPCCDWVGENGAG
                     HFVKMVHNGIEYGDMQLICEAYQLLKDVLGLSAGEMNEIFADWNKGELDSYLIEITRD
                     ILGFIDTDGSALVDKILDTAGQKGTGKWTAIAALDEGQPLTLIGEAVFARCLSAIKEE
                     RVTAAKAFPRTIPAYEGDKAALVESIRKALYASKIVSYAQGYSLMRAAAKTYGWNLNY
                     GGVALMWRGGCIIRSVFLGKIKEAFDRDPALSNLLLDPYFTETVKGCVPAWRKVVAAA
                     VERGVPVPAMSSALAYFDGYTSERLPANLLQAQRDYFGAHTYERVDQPRGQFFHTNWT
                     GEGGDTAASTYTV"
     gene            1063..1914
                     /locus_tag="PLR57_01985"
     CDS             1063..1914
                     /locus_tag="PLR57_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQQ40255.1"
                     /translation="MKLPYKPDFSGKVAVVTGGAGVLCSMFAKALALCGAKVAVLDLA
                     GDKAEALAAELRELGGEAIGVAVNVLETESVQAAHQKVLTSLGPCDILVNGAGGNNAK
                     ATTDDEFFHREESLAEGKKSFFDLDPAGFDFVFGLNLKGTLLPTQAFARDMAEMSAGT
                     IINISSMNAFTPLTKIPAYSAAKAAVSNFTQWLAVYFSQSGIRVNAIAPGFFSTQQNA
                     KLLWNDDGSPTPRTNKILRNTPMGRFGESEELIGALLFLLDNNASSFVTGVVLPVDGG
                     FSAYSGV"
     gene            1920..3218
                     /locus_tag="PLR57_01990"
     CDS             1920..3218
                     /locus_tag="PLR57_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HQQ40256.1"
                     /translation="MELCLRATTESGLTDGELTAAVERLCAEHPANKVLLLPPDFTRY
                     HSKAGFLTNACYHYYAARGASVDILPTLGTHRAMTQAEAMEMFGDVPFERFLVHNWRT
                     GVVKIGEVPGEFLSEITEGLWSDPMNVEVNRRIMDPEYDLILSIGQVVPHEVIGMANH
                     SKNVFVGAGGSDMINKSHMVGAVYGMERMMGRDFTPVRKVFDYALKHFLAERPLVFLL
                     TVTTAPGGKTATHGLFAGRERDALEAAIALAQQKNIDFVEHGIKKCVVYLDPSEFHST
                     WLGNKAVYRTRMAVADGGELLILAPGVETFGEDEQVDALIRKYGYRGRKAVLELFQKP
                     ECEDLRANMGAAAHLIHGSSDGRFTVTYAVQPEMREQIEGVHFRSADINAMLRRYDPA
                     TLKYGYNTLPDGEEIFFIPNPALGLWIDRERFDREGGVLA"
     gene            complement(3392..>3490)
                     /locus_tag="PLR57_01995"
     CDS             complement(3392..>3490)
                     /locus_tag="PLR57_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HQQ40257.1"
                     /translation="RAYDEAGLIGEGTQERFIVENAKFMAKVEQKK"
BASE COUNT          709 a         1034 c         1062 g          685 t
ORIGIN      
        1 tgcgcggccc ctcgatcatg cccggcgggt cgcccgcggc ctggccccac gtcaagccca
       61 tattccaggc tatctcggcc aagaccgaga agggcgaacc ctgctgcgac tgggtgggcg
      121 agaacggcgc ggggcatttc gtcaagatgg tgcacaacgg catcgaatac ggcgacatgc
      181 agctcatctg cgaagcgtat cagctgctca aggacgtgct ggggctcagc gcgggcgaga
      241 tgaacgaaat cttcgcggac tggaacaagg gcgagctgga cagctacctc atcgaaatca
      301 cgcgcgatat tctgggcttt atagatacgg acggttccgc gctagtggac aaaatcctcg
      361 acacggcggg gcagaagggc accggcaagt ggacggccat cgccgcgctg gacgaaggcc
      421 agccgctcac gctgattggc gaagcggtgt tcgcgcgctg tctttccgcc atcaaggaag
      481 agcgcgtaac tgccgccaag gcattcccgc gcacgattcc cgcttacgaa ggcgacaagg
      541 cggctctcgt ggaatccatc cgcaaggcgc tttacgccag caagatcgtc tcctacgcgc
      601 aggggtattc gctcatgcgc gcggcggcaa agacctacgg ctggaacctc aactacggcg
      661 gcgtggcgct catgtggcgc ggcggctgca tcatccgcag tgtgttcctc ggcaaaatca
      721 aggaagcgtt cgaccgcgac cccgcgctga gcaatctctt gctcgacccg tactttaccg
      781 agacggtcaa gggctgcgtt cccgcctggc gcaaggtcgt cgccgccgcc gtggagcgcg
      841 gcgtgcccgt gccggcgatg agttcggcgc tggcgtattt cgacggttac acgagtgagc
      901 ggctcccggc aaacctcctg caggcgcagc gcgactactt tggcgcgcat acctacgagc
      961 gggtcgatca gccgcgcggc caatttttcc acacgaactg gacgggcgag ggcggggata
     1021 ccgccgcgtc tacctacacg gtctgaggag gcgaagattt caatgaaact accttataaa
     1081 ccggactttt cgggcaaggt tgccgtcgtt accggcggcg cgggcgtgct ctgcagcatg
     1141 tttgcaaaag cccttgcgct ctgcggcgcg aaggttgctg ttcttgacct cgcgggggac
     1201 aaagccgaag cgcttgccgc ggaactgcgc gaactcggcg gcgaagcaat cggcgtggcg
     1261 gtcaacgttc tggaaaccga gagcgtgcag gccgcgcatc aaaaggtgct gacatctctc
     1321 ggcccctgcg atatcctcgt taacggcgcg ggcggcaaca acgccaaggc gaccacggac
     1381 gacgaattct tccatcgtga ggaatcgctt gcggaaggca agaagagctt tttcgatctt
     1441 gacccggcgg gctttgattt tgtgttcggt ctcaatctca aaggcacgct tctaccgacg
     1501 caggctttcg cgcgggatat ggcggaaatg agcgctggca cgattattaa catatcttcc
     1561 atgaatgcgt ttacgccgct gacgaagata cccgcctatt ctgccgcgaa ggccgccgta
     1621 tcgaacttta cccagtggct cgccgtgtat ttttcgcagt cgggcatccg cgtcaacgct
     1681 attgcgccgg gctttttctc gacgcagcag aacgcaaagc tgctctggaa cgacgacgga
     1741 agccccacgc cgcgaaccaa caagattttg cgcaacacgc caatggggcg atttggcgag
     1801 tccgaagagc tcatcggcgc gctgctgttt ttgctggaca acaacgcttc cagctttgtc
     1861 accggcgtcg tgctgcccgt tgacggcggg ttctccgcct attcgggagt gtgagccgta
     1921 tggaactctg cctgcgcgcg acaaccgaat ctggcctgac tgacggcgaa ctcaccgcgg
     1981 cggtcgagcg gctctgcgcc gaacatcccg ccaacaaggt gcttttgctg ccgccggact
     2041 ttacgcgcta ccacagcaaa gcggggtttt taaccaacgc ctgctatcac tactatgccg
     2101 cgcgcggcgc gagcgtggac attctgccga cgctcggcac ccatcgcgcc atgacgcagg
     2161 cggaagcgat ggagatgttc ggggatgtac cgtttgagcg cttcttagtt cataattggc
     2221 ggacgggcgt ggtcaagatc ggcgaagtgc cgggcgagtt tctgagcgaa attacggaag
     2281 gcttgtggag cgacccgatg aacgtcgagg tcaaccgccg catcatggat ccggaatacg
     2341 atctcattct ctcgattggt caggtcgtgc cgcacgaggt gatcggcatg gccaaccact
     2401 caaagaacgt ttttgtcggc gccggcggct cggacatgat caacaagtcc catatggtcg
     2461 gcgcggtcta cggcatggag cgcatgatgg ggcgggactt cacgcccgtg cgcaaggtgt
     2521 tcgactatgc gctcaagcac ttcctcgcgg aacgtccgct tgtgttcctg ctgacggtga
     2581 ccaccgcgcc gggcggcaaa accgccacgc acggtctctt tgccgggcgg gagcgggacg
     2641 cgctggaagc ggcaattgcg ctggcgcagc agaagaacat cgattttgtg gagcacggca
     2701 tcaaaaaatg cgtggtctac ctcgaccctt ccgagttcca cagcacatgg ctcggcaaca
     2761 aggccgtcta ccgcacgcgc atggccgttg ccgacggcgg cgagctgctc atcctcgcgc
     2821 ccggcgtgga gacgttcggc gaggacgagc aggtggacgc gctcatccgc aagtatggct
     2881 accgcggccg caaagccgtg ctggagctgt tccaaaagcc ggaatgcgaa gacctccgcg
     2941 ccaacatggg cgcggccgcg caccttatcc acggttcttc cgacgggcgc tttaccgtga
     3001 cctacgcggt gcagccggaa atgcgggagc agatcgaggg cgtacatttc cgctcggcgg
     3061 acataaacgc aatgctgcgc cgctacgacc ccgcaacgct gaagtacggt tacaacaccc
     3121 tgccggacgg cgaggagata ttctttattc cgaaccccgc actggggctc tggatcgacc
     3181 gcgagcgctt tgaccgcgaa ggcggcgtgc tggcgtaaag ggctttcttt actttttctt
     3241 ggaagaaaaa gtaaaacaaa aagaaccgaa ttctctttct ttttcttgga agaaaaagaa
     3301 aacaaaaaga acttcctgat tgggaagttc tttgtttgtt aagagagaaa ttcttttgct
     3361 tcttttcttt taagaaaaga agaagagaag cttatttctt ctgttctact ttcgccataa
     3421 actttgcgtt ctccacgatg aaccgctcct gcgtgccttc gccgatcaac cccgcttcgt
     3481 cgtaggcgcg
//
LOCUS       DAOMGY010000082         3482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_28508_length_3482_cov_3.033849, whole genome shotgun sequence.
ACCESSION   DAOMGY010000082 DAOMGY010000000
VERSION     DAOMGY010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28508_length_3482_cov_3.033849"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2970
                     /gene="nifJ"
                     /locus_tag="PLR57_02000"
     CDS             <1..2970
                     /gene="nifJ"
                     /locus_tag="PLR57_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HQQ40258.1"
                     /translation="RARALNPEHPHQGGTAQNPDIYFQGREAANKYYDAVPGIVQQYM
                     DEVGKVTGRSYHLFDYYGAPDAENILVIMGSGADVAEETIEYLNAKGAKLGLVKVRLY
                     RPFVAEKFVEALPKTTKRIAVLDRTKEPGSLGEPLYQDVINALVETGKLSGIEVVGGR
                     YGLGSKEFTPSMVDAVYANLEEKKPKNHFTVGIVDDVTFTSLPIKRQVNAAPTGTIAC
                     KFYGLGSDGTVGANKNSIKIIGDHTPLYAQGYFAYDSKKSGGLTVSHLRFGKKPIQSP
                     YLIDNADFVACHNPSYVTRYAVAEGIREGGVFLLNSPWTLAEMECELPAVMKRAIAKQ
                     KLRFYNIDAVKLAREVGMGAHINTIMQSAFFKLANVIPAEEAIEYMKAAAKKSYGKKG
                     DEVVKKNWDAIDAGATAIEEIKYPASWADATDGAVPVKTTDDAYFTDYIQPILAQEGD
                     KLPVSKMSPDGTVPTGTTQYEKRGIAVDVPKWIPENCIQCNQCALACPHAVIRPFLVT
                     AEEKAAAPVGFETKKASGKEFDGLEFRIQISPFDCTGCGNCIDVCPAKVKALEFRPLA
                     DAIEKEEENWNYAAKLPEKNLPVNRATVKGSQMLKPLFEFSGACAGCGETPYVKLVTQ
                     LFGDRMVVANATGCSSIYGGSAPTCPYTVNDKGRGPAWANSLFEDNAEFGFGMNLANA
                     HRRENLAALVKKLIAAEYATDAIREAGKEWLEQMDDAQGSRAAADKLIAACEEGVDLS
                     GTEWEAEWQKNGKVCSCDACKLATEVLENKDLLTKQSVWVFGGDGWAYDIGYGGLDHV
                     LAMDEDINVLVMDTELYSNTGGQSSKATPTGSVVKFAAAGKRTKKKDIGLMAMSYGYV
                     YVAKVAMGANPNQLMKAITEAEAYKGPSLIIAYAPCINHGINMGHSQLEEKKAVEAGY
                     WPLYRYNPDLAKEGKNPFTLDSKDPTGSFRDFILGEVRYASLKKAFPDVADELFDRAE
                     KEAKEKIDYYKKLNSME"
     gene            3182..>3482
                     /locus_tag="PLR57_02005"
     CDS             3182..>3482
                     /locus_tag="PLR57_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQQ40259.1"
                     /translation="MAQQNPEKVVIWGDSLAKGVIWNNERRRHGYSRTTAADIVAEKL
                     GVQIINRAKFGYTAPQGLEMMERDFAEGITCDTALLEFGGNDCNFDWAAISEHPEE"
BASE COUNT          851 a         1058 c          990 g          583 t
ORIGIN      
        1 cgcgcccgcg cgctgaaccc cgagcatccg catcagggcg gcaccgcgca gaacccggat
       61 atctacttcc agggccgcga agcagccaac aagtattacg acgcggtacc cggcatcgtt
      121 cagcagtaca tggacgaagt cggcaaggtc acgggccgca gctaccacct cttcgactac
      181 tacggcgcgc cggatgcgga aaacattctc gtcatcatgg gcagcggcgc ggacgtcgcg
      241 gaagaaacca ttgaatacct caacgcaaaa ggcgcaaagc tcggcctcgt gaaggttcgc
      301 ctgtaccgcc cgttcgttgc ggaaaaattt gtcgaagcgc tccccaagac aacaaagcgc
      361 atcgccgttc tcgaccgcac caaggagccc ggctcgcttg gcgaaccgct gtatcaggac
      421 gttatcaacg cgcttgttga aaccggcaag ctctccggca tcgaagttgt cggcggccgc
      481 tacggcctcg gcagcaagga gttcacgccg tccatggtgg acgctgtgta cgccaacctc
      541 gaggagaaga agcccaagaa ccacttcacc gtcggcatcg tggacgacgt aacctttacg
      601 tcgcttccca tcaagcgtca ggtcaacgcc gcgcctacgg gcaccatcgc ctgcaagttc
      661 tacggcctcg gttcggacgg cacggtcggc gcaaacaaga actccatcaa gatcatcggc
      721 gaccatacgc cgctttatgc gcaagggtac tttgcctacg actccaaaaa gtccggcggc
      781 ctcaccgttt cccatctgcg ctttggcaaa aagccgatcc agagcccata cctcattgac
      841 aacgcggact tcgttgcctg ccataacccg tcgtacgtca cccgttacgc cgtggcggaa
      901 ggcatccgcg aaggcggcgt gttcctgctc aacagcccct ggacgctggc cgaaatggaa
      961 tgcgaactgc ccgcggtcat gaagcgcgcg atcgccaagc agaagctgcg tttctacaac
     1021 atcgacgcgg tcaagcttgc gcgtgaagtc ggcatgggtg cgcacatcaa caccatcatg
     1081 cagagcgcgt tcttcaagct cgcaaacgtc attcccgccg aagaagcgat tgagtacatg
     1141 aaagccgccg ccaagaagag ctacggcaag aagggtgacg aagtcgtcaa gaagaactgg
     1201 gacgcaatcg acgctggcgc aaccgccatc gaagagatca agtatcccgc ttcctgggcg
     1261 gacgcaaccg acggcgcagt cccggttaaa accacggacg acgcgtactt caccgattac
     1321 attcagccca tcctcgcgca ggaaggcgac aagctgcccg tctccaagat gagcccggac
     1381 ggcacggttc cgaccggcac gacgcagtac gaaaagcgcg gcatcgccgt ggacgttccc
     1441 aagtggattc cggaaaactg catccagtgc aaccagtgcg cgctggcctg cccgcatgcg
     1501 gtcatccgcc cgttcctcgt taccgcggaa gaaaaagccg ccgctcccgt aggattcgaa
     1561 accaagaagg cctccggcaa agagttcgac ggtctcgaat tccgcatcca gatcagcccg
     1621 ttcgactgca ccggctgcgg caactgcatc gacgtctgcc cggccaaagt caaggcgctg
     1681 gaattccgcc cgctggccga cgccatcgag aaggaagaag aaaactggaa ctacgccgcc
     1741 aaactgccgg agaagaatct gccggtcaac cgtgccaccg tcaagggcag ccagatgctc
     1801 aaaccgctgt ttgagttcag cggcgcctgc gcgggctgcg gcgaaacgcc ttatgtcaaa
     1861 ctggtaacgc agctctttgg cgaccgtatg gtcgttgcca acgcaacggg ctgctcctcc
     1921 atctacggcg gcagcgcgcc gacctgcccc tacacggtca acgacaaggg tcgcggtccg
     1981 gcatgggcaa actcgctgtt tgaggacaac gcggagttcg gcttcggcat gaaccttgcc
     2041 aacgcgcacc gccgcgaaaa cctcgctgct ctcgttaaga aactaatcgc cgcggaatac
     2101 gcgaccgacg ctatccgcga agcgggcaag gaatggctcg agcagatgga tgacgcgcag
     2161 ggttcccgcg cggcggccga caagttgatt gcagcctgcg aagaaggcgt tgacctctcc
     2221 ggcacggagt gggaagcaga atggcagaag aacggcaagg tctgctcctg cgacgcctgc
     2281 aagctcgcca ccgaagtgct tgaaaacaaa gacctgctta ccaaacagtc cgtctgggta
     2341 ttcggcggcg acggctgggc ttacgacatc ggctacggcg gactcgatca cgtgctcgcc
     2401 atggacgaag atatcaacgt tctggttatg gacaccgagc tgtactccaa caccggcggc
     2461 cagtccagca aagcgacccc gaccggctcg gtcgtgaagt tcgccgcggc gggcaagcgc
     2521 accaagaaga aggatatcgg cctcatggca atgagctacg gctatgtgta cgtcgccaaa
     2581 gttgcgatgg gcgcaaaccc caaccagctc atgaaggcca tcacggaagc ggaagcttac
     2641 aagggcccga gcctgattat cgcttacgcg ccctgcatca accacggcat caacatgggc
     2701 cattcgcagc tggaagagaa gaaggccgtc gaagcgggct actggccgct gtaccggtac
     2761 aatcccgacc tcgcgaagga aggcaagaac ccgttcacac tggacagcaa ggatccgacc
     2821 ggcagcttcc gcgatttcat cctcggcgaa gtccgctatg cgtcgctgaa gaaggcgttc
     2881 ccggacgttg cggacgaact gttcgaccgc gcggaaaagg aagcaaagga gaagatcgac
     2941 tactacaaga agctcaacag catggagtaa acgtacatca accaaaacgc cgcgcgggta
     3001 gaccgcgcgg cgcttttttg tttgtgcaaa cgatatggac tgaaaaatgt actcgcagtt
     3061 ttattgcaca aaaagtttta ttttataacc attcaatgac atgatttacc tcgttgactt
     3121 aacaaaaact tgaatcgcat gcgcctataa tagggcaagt aaacagcgag gtatcaacac
     3181 aatggcacag caaaatccag aaaaagtagt tatatgggga gactccctgg caaaaggagt
     3241 catctggaac aacgagcgca gacggcacgg ttattcccgc accacggcgg cggatattgt
     3301 tgcggaaaag ctcggcgtgc agattattaa ccgcgcaaag ttcggctaca ccgcgccgca
     3361 ggggctggag atgatggagc gcgactttgc ggagggcatt acctgcgata cggcgctgct
     3421 ggaattcggc ggcaacgact gcaatttcga ctgggcggcg atctccgagc atccggaaga
     3481 ac
//
LOCUS       DAOMGY010000083         3440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_29010_length_3440_cov_3.380798, whole genome shotgun sequence.
ACCESSION   DAOMGY010000083 DAOMGY010000000
VERSION     DAOMGY010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29010_length_3440_cov_3.380798"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLR57_02010"
     CDS             <1..644
                     /locus_tag="PLR57_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40260.1"
                     /translation="LQRPIMLHAILETALGVFFGGISGYFGKWVDQLIMRIVDIFFCL
                     PGLPILLILSATIASIDAIPPEHRIYYLMGFLTLIGWSGTARLVRGQILMLREQEYMM
                     AAETTGIPARKKIFKHLVPNVMPQLIVTMTLGLGGVILYESTLSYLGLGVPFPRAAWG
                     SMIALADPSKGQEILANYPNMWVPAGLLIIITVLGFSFIGDGLRDAFDPRMKR"
     gene            650..1864
                     /locus_tag="PLR57_02015"
     CDS             650..1864
                     /locus_tag="PLR57_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009534962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40261.1"
                     /translation="MAVRKVFKKKQIKGVDDVAYLSLKEERRISHENAKIMRDFEHRM
                     NRKNVPASEYAATMRDPNNVVEFDDLHTFFYTDIGTVKAVNGVSFDVPRGKTVGVVGE
                     SGCGKSVTSLSLMQLVQAPQGQIVQGQIRFASSDGVCYDIAKMPRSEMLHIRGREISM
                     IFQEPMTSLNPVFTIGEQMDEVILLHEAGMTPQTAKEHSLKMLELVGIAMCDKVYASY
                     PHELSGGMRQRVMIAMALSCNPRLIIADEPTTALDVTIQAQILDLLRDIKRKINGSIM
                     LITHDLGVIAEMADYVVVMYAGRIVEKGTVFEIFDNPKHPYTVGLMRSKPSVSKQIDR
                     LYSIPGQVPNPINMPNHCYFKNRCEHCLERCEGEYPPMVQFSETHFAACYLYEEDPKA
                     EIFESLNSQPKA"
     gene            1874..2851
                     /locus_tag="PLR57_02020"
     CDS             1874..2851
                     /locus_tag="PLR57_02020"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HQQ40262.1"
                     /translation="MEQDNILVVKDLKKYYPITDSIFGKAKAFVKAVDGVSFEIERGT
                     TMGLVGESGCGKTTVGRTILRLHDVTDGTVLFDGEDLAKVSKEHLRRMRPKMQMIFQD
                     PYSSLSPRMPVGEIIGEAVREHNIVPKSQYRSYILKVMRDCGLQPQYFYRYPHEFSGG
                     QRQRICIARAVALNPELIICDEPVSALDVSIQAQIINLMMDLQREFGFTYLFISHDLS
                     VVQHISKTIWVMYLGSMVEMGDKERIFDNPMHPYTKALLSAVPMPDPSYKMNRIILKG
                     DIPSPANPPSGCKFRTRCQQCMDICSEKTPVFKEYEPGHKVACHLYSQD"
     gene            2869..3147
                     /locus_tag="PLR57_02025"
     CDS             2869..3147
                     /locus_tag="PLR57_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40263.1"
                     /translation="MAKRKRDDWEDDGRVIANMDVDGMPYTFRRGKRRNYDAFGKVKQ
                     KSDPIDLTRKEKRAISWGAASAYLLVLGVFVVVFGLFLLFASKVWLNK"
     gene            3237..>3440
                     /locus_tag="PLR57_02030"
     CDS             3237..>3440
                     /locus_tag="PLR57_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HQQ40264.1"
                     /translation="MINEMTARAVLFEAQKSGADYAELYLEDKESTQIEMLKGKVETA
                     NYTRVCGAGVRVLKGTQSTYAYCA"
BASE COUNT          810 a          949 c          957 g          724 t
ORIGIN      
        1 ggctgcagcg cccgatcatg ctccacgcga tcctcgaaac ggcgctgggt gtgttctttg
       61 gcggcatatc gggctatttc ggcaagtggg tcgatcagct gatcatgcgt atcgttgata
      121 ttttcttctg cctgccgggg ctcccgatct tgctgattct ttcggcaacg atcgcttcca
      181 tcgatgcgat tcctccggaa caccgcatct actacctgat gggtttctta acgctcatcg
      241 gctggtcggg tacggcgcgc ctcgtccgcg gccagatcct catgctgcgc gagcaggaat
      301 acatgatggc ggcagaaacg accggcattc ccgcaagaaa gaaaatcttc aaacacctcg
      361 tgccaaacgt catgccgcag ctgattgtaa ccatgacgct cggccttggc ggcgttatcc
      421 tgtatgagtc cacgctgtcc taccttggcc taggcgttcc gttcccgcgc gccgcgtggg
      481 gatcgatgat cgcattggcg gatccttcca aggggcagga aatcctggcc aactatccga
      541 acatgtgggt tccggccggt ctcctcatta tcatcaccgt tctgggattc agtttcatcg
      601 gcgatggtct gcgggacgcg tttgacccga gaatgaagag gtaacggcaa tggcagtgag
      661 aaaggttttt aagaaaaagc agattaaggg tgttgacgac gtagcatacc tttcgttgaa
      721 ggaagaacgt cgcatcagcc atgagaacgc aaagatcatg cgtgactttg agcaccgcat
      781 gaaccgcaaa aacgttccgg cttcggaata cgcggcaacg atgcgcgatc ccaacaacgt
      841 tgtggagttt gacgacctgc atacgttctt ctatacagat atcggcacgg ttaaggccgt
      901 caacggcgtg agcttcgatg ttccgcgcgg caaaaccgtc ggcgtcgttg gcgagagcgg
      961 ctgcggcaaa tccgtaacca gtctctcgct catgcagctc gtgcaggcac cgcagggcca
     1021 aatcgtgcag gggcagatcc gctttgcttc cagcgacggc gtatgctacg acattgcgaa
     1081 aatgccgcgt tcggaaatgc tgcatatccg cgggcgcgaa atctccatga tcttccagga
     1141 gcccatgacg agcctcaacc cggtgttcac catcggcgag cagatggacg aagtcatcct
     1201 tctccacgaa gccggcatga caccgcagac ggcgaaagag cattcgctta aaatgctcga
     1261 actcgtcggc attgccatgt gcgacaaagt ttacgcaagc tatccgcacg agctttccgg
     1321 cggcatgcgc cagcgcgtca tgattgctat ggcgcttagc tgcaacccgc gcctgatcat
     1381 tgcggacgaa ccgaccaccg cgctggatgt taccattcag gcgcagattc tcgatttgct
     1441 gcgcgatatc aagcgcaaga tcaacggcag catcatgctc attacgcacg accttggcgt
     1501 tatcgccgaa atggcggatt acgtggtcgt tatgtatgcc ggacggatcg ttgaaaaggg
     1561 tacggtgttc gaaatcttcg ataaccccaa gcatccctac accgtgggac tcatgcgcag
     1621 taaacccagc gtgagcaaac agatcgatcg tctttacagc atccccgggc aggttccgaa
     1681 ccccatcaac atgcccaacc actgctattt caagaaccgc tgcgagcatt gcctggagcg
     1741 gtgcgagggc gagtacccgc ccatggttca gttcagcgaa acgcatttcg ccgcctgcta
     1801 tctctatgag gaagacccca aggcggagat tttcgaatcg ctcaacagcc agccgaaggc
     1861 gtgaggagga agcatggaac aagataacat cctggtcgtc aaagacctga aaaaatacta
     1921 tccgatcacc gattcaatct tcggtaaggc aaaggcattc gtcaaagcgg tcgacggcgt
     1981 ttcgttcgaa attgaacgcg gcaccacgat ggggctcgtc ggcgagagcg gctgcggcaa
     2041 aacgacggta ggacgcacga ttctgcgcct gcacgacgta accgacggaa cggttctctt
     2101 cgacggcgaa gacctcgcaa aggtatcgaa agagcacctg cggcggatgc gccccaagat
     2161 gcagatgatc tttcaggatc cgtattcgag cctttcgccg cggatgccgg ttggcgagat
     2221 catcggcgaa gcggtacgcg agcacaacat cgtaccaaag agccagtacc gctcctacat
     2281 tctgaaagtc atgcgcgact gcggactgca gccgcagtac ttctaccggt atccccacga
     2341 attctccggc ggccagcggc agagaatctg catcgcccgc gcggtggcgc tcaatcctga
     2401 gctcatcatc tgcgacgaac cggtcagcgc tctggacgtt tcaattcagg cgcagattat
     2461 caacctcatg atggacctgc agagggagtt cgggtttacc tacctgttta tttcgcacga
     2521 tctctcggtg gttcagcaca tcagcaagac catctgggtt atgtatctcg gttccatggt
     2581 ggagatgggc gataaggaac gcatcttcga caatccgatg catccgtata ccaaggcgct
     2641 gctcagcgcg gtgcccatgc ccgatccttc ctataagatg aatcgcatca tcctcaaggg
     2701 tgacattccg agccctgcga acccgccttc cggctgcaaa ttccgcaccc gctgccagca
     2761 gtgcatggat atctgctcgg aaaaaacgcc ggtgttcaag gaatacgaac cgggacataa
     2821 ggtggcttgc catctttaca gtcaggattg accaaaggag cgttgaagat ggcgaaacgc
     2881 aagcgcgacg attgggaaga cgacggacgc gtcattgcca acatggatgt ggacggcatg
     2941 ccgtatacct tccgccgcgg gaagcgcagg aactacgacg cgttcggtaa agtcaagcag
     3001 aaatccgatc cgatcgattt gaccagaaag gaaaaacgag ccatctcctg gggcgcggct
     3061 tccgcctatc tgcttgtgct gggtgtattc gtcgttgtat ttgggctgtt tctgctgttt
     3121 gccagcaaag tttggctcaa taaataaata cgaagaaagc cgtttccaag aaacggcttt
     3181 tctttgcggg atattaaact tatgacaata aacactgacg gaggagggaa cattctttga
     3241 tcaacgaaat gaccgcgcgg gccgtgcttt ttgaagcgca gaagagcggc gcggattatg
     3301 cggagctcta tttggaggac aaagagagca cgcagattga aatgctgaag ggcaaagtgg
     3361 aaaccgccaa ctacacgcgt gtctgcggcg cgggcgtacg cgtgcttaaa ggcacgcaga
     3421 gcacatacgc ctattgcgcg
//
LOCUS       DAOMGY010000084         3434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_29066_length_3434_cov_2.550459, whole genome shotgun sequence.
ACCESSION   DAOMGY010000084 DAOMGY010000000
VERSION     DAOMGY010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29066_length_3434_cov_2.550459"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(691..1251)
                     /locus_tag="PLR57_02035"
     CDS             complement(691..1251)
                     /locus_tag="PLR57_02035"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HQQ40265.1"
                     /translation="MILLVAAALGVVLGLVSGGSIRRCAKYPLYGAALPILALLIKAC
                     AAYLLEPQTLAETVCILQYALVFSFLLFNAKHGLWPLLAFLGAFSNFVVILLNGGCMP
                     VAASLMGTDAARIALLHAGKIYAYSAITERTVLPFLGDVLRLGPAGMPFGFASVGDIV
                     LCAGVALLCFQMTRCKERSQPESKPV"
     gene            complement(1248..2570)
                     /locus_tag="PLR57_02040"
     CDS             complement(1248..2570)
                     /locus_tag="PLR57_02040"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HQQ40266.1"
                     /translation="MKKQQIYTTFISLCGIGVFGISVYQSIALFTDPQTSLSMILFSA
                     VMLVIMLLCHMLPIYITSDKTMEISFVAVIACVVTKGVALSIVLFALSTVLALLQDTK
                     TRKYYSPWARAPLKELFNVSNVLVSNWIGGILYNWIVPDAGGEVFAWRVMIGAVVFSC
                     VAIFINLVLFILYYKLSEQGNFFMLLRDNIGGILPNIVATIPLGLLIGYILTQTNAYL
                     WLVLFMAPLMLARYSFKIYLDSHTILLRTIASLVEAIEAKDQYTRGHSQRVAYISNAL
                     CRALKCPKKFTDQVTMAALLHDTGKIGVEDAILRKPGPLTKEEYDIVKQHPVIGRRII
                     ESINLPQTVNDAVLYHHRRFDGTGYPEEGPAAGELPLSAAILAVADCYDAMITDRPYR
                     KGMTKAEARKIIEEVAGTQLDPKVVKAFLAIEPQLKPEEAETQLLYAI"
     gene            complement(2921..>3434)
                     /locus_tag="PLR57_02045"
     CDS             complement(2921..>3434)
                     /locus_tag="PLR57_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40267.1"
                     /translation="SRRNGKSLVRDVQSAITARYGIEVDYRIISVAQIAPSVMSGMGF
                     RLTYAGINVRTSDKNVDVSVTLRRDTEEFTGTAHGGSFQFSRMRSIAQATLDAVKACT
                     GGTSFFELASTELVRSNGRPIVITQIFCVPDLTPLIGAVYAEADADAAVVQSVLDAIN
                     RKLEIYTQEM"
BASE COUNT          830 a          922 c          876 g          806 t
ORIGIN      
        1 cgttacgatt gctaaaatcg gaatgagctt ctgcgaaagc tgcaatttgt agtcgtcgta
       61 cgcagtgtgc gtaatctgcg cgcccccggc gctgcctgcc gattgcatcc tgttcaaatg
      121 ttttcacccg tcgagcagct attcataaga tagaaaaaca agatgttcgt atcatcagca
      181 aattcgatgt atatacgacc catcttttct ctattctaca ccaatgaatt gttatttcaa
      241 tatttttcca aaaattttag ctcattttcc agttctgttt gaacaaatct gtgttccaca
      301 gagcgcaaca cgcagcagtc tatatttttc gacaaaatcc gagcaaattt gcaggtattg
      361 cgggtaaaaa agttccttct ccgggaataa ccattccatc ccatgttcgg ttcctatcgt
      421 gcttgaccgc atagtaagcc gtcgataata taccatgcat ggtgtttttc agtttttagg
      481 tgcttgaaga atcgtctttt aaatacgcgt tcatttagtt tgtctatcat atattgttgt
      541 attccgattg ctatcttaag aatattgtgg ttgcctgtca tcctgcatca gcagagatac
      601 gctgcgtggt atttttgttt ttctttacaa aaaaagcgca cccgataacc agcgcgctga
      661 aattacgaat tctcacatat actaacccag ttataccggt ttgctttccg gctgagaacg
      721 ctccttgcat ctcgtcatct ggaagcataa cagcgcgacg cccgcgcaga gcacgatatc
      781 ccccacgctc gcaaacccga acggcatccc ggccgggcct aagcgcagaa catcacccag
      841 aaacggcaga acggtccgct ccgtaattgc ggaataggcg taaattttac ccgcgtggag
      901 cagcgctatt ctggcagcgt ccgttcccat cagggacgcc gcaaccggca tgcagccgcc
      961 gttgagcagg ataaccacga agttggaaaa agcgcccaga aacgcgagca gcggccaaag
     1021 gccgtgtttt gcgttgaaca acagaaaaga gaataccagc gcgtactgca ggatgcagac
     1081 cgtctccgcc agcgtctgcg gttccagcag gtaagcagcg caagccttga tcagcagcgc
     1141 cagaatcggc agcgccgcgc cgtagagcgg atactttgcg cagcgcctga tggatccgcc
     1201 gctgacaaga cccagcacaa cgcccagagc tgccgcgacg agcagaatca tatggcgtac
     1261 agaagctgcg tttccgcttc ttccggtttc agctgcggtt caatggccaa aaatgctttt
     1321 acaaccttgg ggtcaagctg cgtgcccgcc acttcttcaa taatcttccg cgcttccgcc
     1381 ttggtcatgc ccttacggta aggacggtcg gtaatcattg cgtcgtagca gtctgcaacc
     1441 gcaagaatcg ccgccgaaag aggcagttct cccgcggcgg gtccttcttc gggatagccc
     1501 gttccgtcga atcttctatg atggtaaagc accgcgtcgt tgaccgtctg cggcaggttg
     1561 atgctctcaa tgatgcgcct gccgatgacc gggtgttgtt tgacgatgtc gtactcttcc
     1621 ttagtcagcg ggccgggttt acggaggata gcgtcctcaa cgccgatttt gccggtatcg
     1681 tgcagaagcg ccgccatcgt cacctgatcg gtgaacttct tggggcattt gagcgctctg
     1741 cagagagcgt tgctgatata ggcaacgcgt tgggaatgac cgcgcgtata ttgatccttg
     1801 gcctcaatcg cttctacaag ggatgcgatg gtgcggagca ggatcgtgtg gctgtccaga
     1861 tagattttaa acgaataccg cgccagcatc agaggagcca tgaacagcac cagccaaagg
     1921 taggcgttgg tttgcgtcag aatgtagccg attaagagac cgagcggaat ggtcgctaca
     1981 atgttcggca gaatgccgcc gatgttgtcc cgcagcagca tgaaaaagtt gccctgctcg
     2041 ctgagtttgt agtagaggat gaaaagaacg aggttgatga agattgccac acaggaaaaa
     2101 acgaccgccc cgatcatgac gcgccaggca aacacctcgc cgcccgcgtc cggaacgatc
     2161 cagttataga gaatcccgcc tatccagttg gaaacgagaa cgttggaaac gttaaagagc
     2221 tctttgagcg gtgcgcgcgc ccagggggaa taatacttgc gtgttttcgt atcctgcagc
     2281 agcgccagca cggtggaaag cgcaaacagc acgatggaaa gcgcaacgcc cttggtcacg
     2341 acacaggcaa tcaccgcgac aaaactgatc tccatggttt tgtcggaggt gatgtaaatg
     2401 ggcagcatgt ggcacagaag catgatcaca agcatgaccg cgctgaaaag aatcatgctc
     2461 agcgacgttt gcgggtctgt aaagagcgcg atggattggt atacgctgat accaaacacc
     2521 ccgataccac acagactgat gaacgtcgtg taaatttgct gcttcttcat ttcataccct
     2581 catgccgtaa cccgccgggc gggtaaaaca ttaacaggtc cggcgagaga ttcatgagtt
     2641 tcctttaccg tgctacttgt cttatctgac gaagaagtac gcgccgctgg agagaaccag
     2701 agccgcaagg gaagcaagcg ccatgataat ctttttcatt ggatatagcc tccttcttta
     2761 ggtctctgtt cgccgctcgt tgaacaggct atatccgcta cgctattgcc ggacctgtta
     2821 agttttcaag atacctcaat caatctgaat ttcgatcttt cccatcgatc gacgaatgtg
     2881 caaacgtaaa tctctaatat aaaaggaaaa aactcgtttc ttacatctcc tgcgtataga
     2941 tttcgagttt ccggttaatc gcgtccaata cgctctgcac aaccgccgca tccgcatccg
     3001 cttccgcata gacggcgccg atgagcggtg taagatccgg cacgcagaaa atctgggtga
     3061 tcacgatcgg gcgtccgttg gagcgcacca gctccgtgct ggccagctca aaaaacgaag
     3121 tcccgcccgt acaggccttg accgcgtcca gcgttgcctg cgcaatggag cgcatcctgg
     3181 aaaactgaaa cgatccgccg tgcgctgtgc cggtgaattc ttcggtatcc cggcgcaggg
     3241 taacggaaac atccacattc ttatcggagg tgcggacgtt gattcccgca tatgtcagcc
     3301 ggaagcccat gccggacatc acgcttggcg caatctgcgc cacgctgatg atgcggtagt
     3361 ccacctcaat gccgtaccgc gccgtaatgg cggactgcac gtcccgtacg agcgattttc
     3421 cgttgcggcg gctg
//
LOCUS       DAOMGY010000085         3426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_29159_length_3426_cov_2.883714, whole genome shotgun sequence.
ACCESSION   DAOMGY010000085 DAOMGY010000000
VERSION     DAOMGY010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29159_length_3426_cov_2.883714"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /gene="modA"
                     /locus_tag="PLR57_02050"
     CDS             <1..848
                     /gene="modA"
                     /locus_tag="PLR57_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQQ40268.1"
                     /translation="PAATEAPAVTEAVATEEPDATEEPAATAAPLEAAEVQLFIAASL
                     EGAFQEIIPLYNATQPNVTITYNADSSGKLLTQIQEGFACDVFFSAATSQVKALEEGG
                     QMIPDTRVDLLCNKLVLVTLKGSGTAVTGFADLASAKSIALADGSVPAGKYARQVLIN
                     LGILDGSKAAADYTTSEVSTALGGVEINECSSVSKVKEAVKEGSNEVGLVYYSDAYSI
                     KDDVDILETADSSLTGDIIYPVCRVNNAEATAAETAAAEDFVAFLQTDAAKTVFEKFM
                     FVIYE"
     gene            1038..1742
                     /gene="modB"
                     /locus_tag="PLR57_02055"
     CDS             1038..1742
                     /gene="modB"
                     /locus_tag="PLR57_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HQQ40269.1"
                     /translation="MEQLIEILRSLDYSPLWISLKTGVVATVLCFFLGIWAARGVMRM
                     GKRAKAVVDGLLTLPMVLPPTVAGFFLLLIFSLRRPIGTFIYATFSIKVVQSWLGCIL
                     AASVIALPLMYRNARAAFEQVDIELIYAARTLGMSERKIFWRVALPSAGPGVVSGTIL
                     TFARALGEYGATSMLAGNIAGKTGTISQRIAMVIQNGDYLTAGVWVVVILIISFAIVA
                     AMNLFADSKLKNIKKW"
     gene            1786..2853
                     /locus_tag="PLR57_02060"
     CDS             1786..2853
                     /locus_tag="PLR57_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016324357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ40270.1"
                     /translation="MSLEVSIHKKLDGFSLDVSFTAEPGVTALLGASGSGKSMTLRCI
                     AGVEKPDAGRIVLDGRVLYDSERKIDLPPQQRRVGYLFQRYALFPNMTVLQNIEAGLS
                     LEHEKSVRMQKRESLIKRFHLEGLERRYPRELSGGQMQRVALCRMLAGEPEAILLDEP
                     FAALDSYLRWQLEREVKNTLAEFSGVSLLVSHDRGEVYRMSERVCVLGGGMSERVTST
                     RELFEHPRTVQSALISGCKNVVPAEMAGDCDVFVPDWNAHLSCEPAGERRITHVGVRA
                     HNIALCERDAENALLCRVEDVAEDVLSCVLTLRPEGAAATLRAELTKEAAKPLPVGSE
                     AYFSIPPAAVMLLSESEQAGS"
     gene            2931..>3426
                     /locus_tag="PLR57_02065"
     CDS             2931..>3426
                     /locus_tag="PLR57_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HQQ40271.1"
                     /translation="MFKASVITVSDKGYAGERVDTAGPLAAELLREAGYDVLTVSIVP
                     DERSLIEAELRRHADELGAALVLTTGGTGFSQRDVTPEATLAVCERLVPGIPEAMRAY
                     CLPITNRAMLSRAAAGIYHETLIVNLPGSPKAVKENLAPVLPALEHGLCMLLGTKNEC
                     ASEPR"
BASE COUNT          705 a          960 c         1084 g          677 t
ORIGIN      
        1 aacccgcagc gacggaagca cccgcagtga cggaagcagt tgcaacggaa gaacccgatg
       61 caacggaaga acccgcggca acagcggctc cgctggaagc ggcggaagtg cagctcttta
      121 tcgcggcaag ccttgaaggc gcgtttcagg agattattcc gctctataac gcgacgcagc
      181 cgaatgtgac catcacctac aacgcggaca gctccggcaa gctgctcacg cagattcagg
      241 aagggtttgc ctgcgacgtg ttcttttccg cagcgacctc tcaggtaaaa gcgctggaag
      301 aaggcggcca aatgattccc gacacgcgcg tcgacctgct ctgcaacaag ctcgtgctcg
      361 ttaccctgaa aggcagcggc acggcggtga ccggctttgc ggatcttgcc agcgcaaaga
      421 gcatcgcgct tgcggacggc agcgttcccg cgggtaaata cgcccgtcag gtgctgatta
      481 acctcggcat tctcgacggc agcaaagcgg cggcggacta taccacctcg gaagtctcaa
      541 cggctctcgg cggcgtggaa atcaacgagt gcagcagcgt cagcaaggtc aaggaagcgg
      601 tcaaggaagg ctccaacgaa gtcggcctcg tgtattactc cgacgcgtac tcgattaaag
      661 acgatgtgga tatcctcgaa acggcggatt cttcgctgac gggcgatatc atctacccgg
      721 tctgccgcgt gaacaacgcc gaagcgaccg cagccgaaac ggccgccgcg gaagactttg
      781 tagcgttcct gcagacggac gcggcgaaga ctgtcttcga aaaattcatg tttgtcattt
      841 acgaataagc gaccctccgc aacattcgta atgcatggcg cgccacccca aacggcgctc
      901 cacgtgcccg ccgtcatgcg gtttggcggc gggcatttct tttacagcat accggaaccg
      961 aaccgcagag ataaaacagc gcggctgttc ggtaaatatg atatgatgaa cgcaagccgg
     1021 atttcgaggt gaaagccatg gagcaactca tcgaaatact gcggtcgctg gactacagcc
     1081 cgctctggat ctcgctcaaa acgggcgtgg tcgcaaccgt gctgtgcttt tttctgggca
     1141 tttgggcggc gcggggcgtc atgcgtatgg gtaagcgcgc caaagccgtt gtggacgggc
     1201 tcttgacgct gccaatggtg ctgccgccga cggtcgcggg tttcttttta ctgctcatct
     1261 tcagccttcg acggccaatc ggcacgttta tttacgctac gttctccatt aaagtcgtgc
     1321 agagctggct cggttgcatc cttgccgcca gcgtaattgc gctgccgctg atgtaccgta
     1381 acgcgcgcgc cgcgtttgaa caggtggata ttgagctcat ctacgccgcg cggacgctcg
     1441 gcatgtccga acgcaagatc ttctggcgcg tggcgctgcc ttccgcgggt cccggcgtgg
     1501 tcagcggaac gattttaacg tttgcgcgcg cgcttggtga atacggcgcc acgagcatgc
     1561 tcgcgggaaa catcgcggga aagaccggca cgatttccca gcgcatcgcc atggtgattc
     1621 aaaacggtga ctacctcaca gcgggcgttt gggttgtcgt gatactgatt atttcgtttg
     1681 ccatcgtcgc ggctatgaac ctgtttgcgg attcgaagct gaagaatata aaaaagtggt
     1741 gattgcccgt tttttcacgg gtgaagaata ggaaaagagc gaacgatgtc gctggaagtt
     1801 tcgattcata aaaaactgga cggattttca ctggatgtgt cgtttacggc cgaaccgggc
     1861 gtgacggcgc tgctcggcgc ttccggcagc ggcaaaagca tgacacttcg gtgcatcgca
     1921 ggtgtggaaa agccggatgc ggggcgaatc gttctcgacg gacgcgtgct ctacgattcc
     1981 gaacgtaaga tcgacctgcc gccgcagcaa cggcgcgtgg ggtatctgtt tcaaaggtac
     2041 gcgctgtttc ccaacatgac ggtgctgcag aatattgaag cgggattgtc gctggagcat
     2101 gaaaaatccg tccgcatgca aaagcgggag agcttgatta aacggtttca tctcgaaggg
     2161 ctggagcgcc gctacccgcg cgagctttcc ggcgggcaga tgcagcgcgt ggcgctctgc
     2221 cgcatgctcg cgggcgagcc ggaggcgatt ctgctggacg agccgttcgc cgcgctggac
     2281 agctaccttc gctggcagct cgagcgcgag gtgaaaaata cgcttgcgga gttttccggc
     2341 gtgtcgctgc tcgtttccca cgaccggggc gaggtttacc gcatgagcga acgcgtctgc
     2401 gtgctcggcg gcggaatgag cgaacgggtc acgagcacgc gcgaactctt tgagcatccc
     2461 cgcaccgtgc agagcgcgct gatctccggt tgcaaaaacg tggttcccgc cgagatggcg
     2521 ggggattgtg atgtattcgt gccggactgg aacgcgcacc tttcctgcga acccgcaggt
     2581 gaacggcgga tcacgcacgt gggtgttcgc gcgcataaca tagcgctttg cgaaagagac
     2641 gcggaaaacg ctttgctctg ccgcgtggaa gatgttgcgg aggatgtgct ttcctgcgtt
     2701 ttgacgttgc gccccgaagg cgcggcggcg acgctccgcg cggagttgac aaaagaggcg
     2761 gcaaagccgc tccccgtcgg ttcggaggcg tatttttcaa ttcccccggc ggcggtcatg
     2821 ctgctgtcgg agtcggaaca ggcgggttct tagggctgcc taagccttga gcaggggttt
     2881 ggggacggag tccccataaa aaagattcca acattcttgg aggtagatgt atgtttaagg
     2941 catccgtcat tacggtgagc gacaaaggct acgcgggcga gcgtgtggac accgccggcc
     3001 cgctggcggc ggaactgctc cgcgaagcgg ggtacgacgt gctgaccgtt tccatcgtgc
     3061 cggacgagcg gtcgctcatt gaagcggaac tgcgccgcca cgcggacgag ctgggcgcgg
     3121 cgctcgtgct caccacgggt ggaacgggct ttagtcagcg ggacgttacg ccggaggcga
     3181 cgcttgccgt ctgcgagcgg ctcgtgccgg gcattccgga agcgatgcga gcctattgtt
     3241 tgccgattac caaccgcgcc atgctctcaa gagctgcggc gggcatctac cacgaaacgc
     3301 tgattgtcaa cctgccgggc agccccaagg cggtcaagga aaacctcgcg cccgttttgc
     3361 ccgcgctgga gcacgggctt tgcatgctgt taggcacaaa gaacgagtgc gcaagcgaac
     3421 caagaa
//
LOCUS       DAOMGY010000086         3409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_29359_length_3409_cov_3.844961, whole genome shotgun sequence.
ACCESSION   DAOMGY010000086 DAOMGY010000000
VERSION     DAOMGY010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29359_length_3409_cov_3.844961"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PLR57_02070"
     CDS             <1..1101
                     /locus_tag="PLR57_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQQ40272.1"
                     /translation="QIAVITRNMEFRKLSVTTLGATIFSGIVGIWMAYAGYGIWALVV
                     NQLLGSLGTLILLWLQDRWKPLLTISLPRVKSLFSYGWKLLVSSLLDTGYTELSSLVI
                     GKRFIGDALAFYNRGRQYPEMIANNLTSVALAVLFPAYSQKQDDQPLVLEMVRKTNRT
                     TALMVFPMMAGMAMVATLLMRVLLTEKWVPAAPFLQVLAIVYALYPVEAADLQAINAI
                     GRSDLYLKTEIIKKAFGILALAAAVFLFTTPIAIAFSAALTAVFSMIVTMIVMKRLFF
                     YRMRDQIWDMMPPILLSGAMAAAVWGVSLIQMGDLTSLIVQVVCGIAVYLGLAVLCKL
                     KSLDYLVASIKDFLNRKKTKQPGQDEPKKDQL"
     gene            1179..2468
                     /locus_tag="PLR57_02075"
     CDS             1179..2468
                     /locus_tag="PLR57_02075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HQQ40273.1"
                     /translation="MKLAVAGTGYVGLVAGVCFAEKGHTVTCVDVDEQKVARMRGGES
                     PIYEVGLEELLKKNIAANRLFFTTDYASAYADADAIFIGVGTPELADGSANLSYIAAV
                     CREIAESVTRDCLVVVKSTVPVGTNDKVEQYIKDNLIRDVKVRVASNPEFLAQGNAVH
                     DTMHATRIIIGTEDEEAERTLLKIYAPFDLPIVSVSRRSAEMIKYACNNFLALKISYM
                     NDIANLCERVGANVDDVAEGMRYDPRIGAKFLKAGVGYGGSCFPKDTKALCFLAEQHG
                     YHLRTVDAAVAINAEQKTKLFEKAKKRFMSFQNLRVAVLGLAFKPGTDDLREAPALDN
                     VKLLLDHGAQVICYDPVAGENFKKKFPNVTLAASAEEALAGAFCCFVFTEWEEIVRLS
                     PETFRSKMHVPLVYDGRNIFDQDTMKRGGVEYYSIGR"
     gene            2528..>3409
                     /locus_tag="PLR57_02080"
     CDS             2528..>3409
                     /locus_tag="PLR57_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ40274.1"
                     /translation="MIKQTEKPILVTGAAGFIGYHLAKRLLNAGYAVAGIDNLNDYYD
                     PALKRARLDELASFEQFSFTEGDLADESTVEEIFARVQPEIVVNLAAQAGVRYSIDHP
                     RAYIESNILGFFNVLEAVRRYPVKHLLYASSSSVYGDREQTPFSVSDRVDEPISLYAA
                     TKKSNELFAYTYSHLYGIPATGLRFFTVYGPFGRPDMAYFKFTKAILAGETIDVFNNG
                     ELLRDFTYVDDVTACVEAMLFSPPKPNANGDRHAVYNIGNNKPEKLMDFIQTLESALG
                     VTANKRFLPMQPGDVTQT"
BASE COUNT          746 a          938 c          999 g          726 t
ORIGIN      
        1 cagattgccg ttattacccg caatatggag tttcgaaagc tcagcgttac aacgctggga
       61 gcgaccatct tttccggtat cgtcggcatc tggatggcgt acgctggcta cggtatctgg
      121 gcgctggtgg tcaaccagtt gctcggttct ctcggaacgc tgattttgct ctggctgcag
      181 gaccgctgga aaccgctttt gaccatatcg cttccgcgcg tcaagtcgct gttcagttac
      241 ggctggaaac tgctggtttc gtcgcttctg gatacggggt atacggagct ttcgtcgctg
      301 gtcatcggca aacgcttcat cggcgatgcg ctggcgtttt acaaccgcgg gcggcaatat
      361 cccgagatga ttgccaataa cctcaccagc gtggcgcttg ccgtgctgtt tccggcatac
      421 tcccaaaagc aggacgatca gccgctcgta ctcgaaatgg tacgaaagac gaaccgcacc
      481 accgcgttga tggtgtttcc catgatggcg ggtatggcga tggtggcgac gcttctaatg
      541 cgcgtactcc tgacggagaa gtgggtgcct gccgcgccgt ttctgcaggt actggccatc
      601 gtttacgcgc tgtatcccgt tgaagcggcg gatctgcagg cgatcaacgc catcggacga
      661 agcgacctgt acctaaaaac agagatcatc aagaaggctt tcggcattct ggcattggcg
      721 gcggcggttt tcctctttac aacgcccatt gctattgcgt tttccgccgc gctgacggct
      781 gttttctcca tgatcgtgac catgatcgtg atgaagcggc tgttctttta ccgcatgcgc
      841 gaccagattt gggatatgat gccgccgatt ctgctctccg gcgcgatggc tgccgccgtc
      901 tggggcgttt cgctgataca gatgggcgat ttgacttcgc tgatcgtgca ggtcgtttgc
      961 ggtattgcgg tttacctcgg tttggcggta ttatgtaagt taaagagtct ggattatctg
     1021 gttgcttcga tcaaggattt tctgaacaga aagaaaacga agcagcccgg gcaggacgaa
     1081 ccaaagaaag accaactata ataaataaag aaacgaatca gtactgcatc cgcccgcaca
     1141 ggatagaacg cgcggcggag caacgaggag gattctgcat gaaactggcg gtagccggaa
     1201 cgggatatgt cgggttggtg gcgggcgtct gctttgccga aaaggggcat acggtcacct
     1261 gtgtagacgt ggacgagcag aaagttgcca gaatgcgcgg cggagagtca cccatctacg
     1321 aagttgggct cgaagagctg cttaaaaaga acatcgcagc aaaccgcctg ttctttacca
     1381 ccgattatgc cagcgcctat gcggacgcgg acgccatctt catcggcgtc ggcacgcctg
     1441 aactggcgga cggcagcgcg aacctttcgt atatcgcggc ggtctgccgg gagattgcgg
     1501 aaagcgtcac gcgcgactgc ctcgtggtcg ttaagtcaac cgtgccggtc ggcacgaacg
     1561 ataaagttga acagtatatc aaggacaacc tcatccgcga cgtaaaagtc cgcgtggcga
     1621 gcaatccgga attccttgcg cagggcaacg cggtgcacga caccatgcac gccacgcgca
     1681 ttatcatcgg caccgaggac gaggaagccg agcggacgct gcttaaaatc tacgcgccgt
     1741 tcgacctgcc catcgtttcg gtttcccgcc gttcggcgga gatgatcaag tacgcctgca
     1801 acaacttcct cgcgctcaag atttcgtaca tgaacgacat cgcaaacctc tgcgaacgcg
     1861 ttggcgcgaa cgtggacgac gtagccgaag gcatgcggta cgacccgcgc atcggggcaa
     1921 agttcctcaa ggcaggcgta ggctacggcg gaagctgttt ccccaaggac accaaagcgc
     1981 tgtgcttcct cgccgaacag catggctacc atctgagaac cgtggacgcg gcggtagcga
     2041 tcaacgccga gcagaagacc aagctcttcg aaaaagcgaa aaagcggttt atgtccttcc
     2101 aaaaccttcg cgtggcggtg ctggggcttg cgttcaagcc cggtacggac gatttgcggg
     2161 aagcgcccgc actcgacaac gtcaagctcc tgctggatca cggcgcgcag gtgatctgct
     2221 acgacccggt cgcgggtgag aactttaaaa agaaattccc gaacgttacc cttgccgctt
     2281 ccgctgagga ggcgcttgct ggcgcgttct gctgctttgt gtttaccgag tgggaggaga
     2341 tcgtccggct ctcgccggag acgttccgct ccaagatgca tgtgccgctg gtatatgacg
     2401 ggcggaacat cttcgatcag gatacgatga agcgcggcgg cgtggagtac tactccatcg
     2461 gcagatagtc catctgtcaa ccggtgcgcg agtgaaaaat gtttactgca gtgaaggcgg
     2521 ataaagagtg atcaagcaga ccgaaaaacc gattctcgta acgggggcgg ccgggttcat
     2581 cggctaccat ctggcaaaac gcctgcttaa cgcaggatac gcagttgcgg gaatcgacaa
     2641 cctcaacgac tattacgacc ccgcgctcaa gcgcgcgcgg ctggacgagc ttgcttcgtt
     2701 tgaacagttt tcgtttacag aaggcgatct ggcggacgaa tccacggtgg aagagatctt
     2761 tgcccgcgtt cagccggaaa ttgtcgtcaa ccttgccgcg caggcgggcg ttcgctacag
     2821 catagaccat ccacgggctt atatcgagag caacatcctt ggctttttca acgtgctgga
     2881 ggccgtccgg cgctatccgg tcaagcacct gctgtacgct tccagctcgt ccgtctacgg
     2941 cgacagggaa cagacgccgt tttccgtttc ggaccgcgtg gacgagccga tttcgctcta
     3001 cgccgcgacg aaaaagagca acgagctgtt tgcctatacc tattcgcacc tttacggcat
     3061 ccccgcaacg gggctgcggt tcttcaccgt gtacggcccg ttcggaaggc cggatatggc
     3121 gtatttcaag tttaccaaag cgattctcgc gggggagacc atcgacgtat tcaacaacgg
     3181 cgaactcctg cgcgatttta cgtatgtgga cgacgtgacc gcctgcgtgg aagccatgct
     3241 tttttcgccg ccaaagccga acgcaaatgg agaccggcac gcggtctaca acatcggcaa
     3301 caataagcct gaaaagctca tggatttcat ccagacgctg gaaagcgcgc ttggcgtaac
     3361 cgcaaacaag cggtttttgc ccatgcagcc gggggatgtg acgcagacg
//
LOCUS       DAOMGY010000087         3404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_29436_length_3404_cov_3.154374, whole genome shotgun sequence.
ACCESSION   DAOMGY010000087 DAOMGY010000000
VERSION     DAOMGY010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29436_length_3404_cov_3.154374"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLR57_02085"
     CDS             complement(<1..494)
                     /locus_tag="PLR57_02085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ40275.1"
                     /translation="MELQGLYRIQKKDLAKCAEVSARAFITDPALIYQFGGKIPSYRR
                     LKRYFDVIFQTGFPYYDFFAPSENIEGVIGLLPPNTDSVPPMEFLLRGGWKMPLTVCR
                     GLLGRLDKYEIHSLEIRKAVHAMDAWYVMMLAVDPEEQGKGHGAKMLRAFLAEMDAQK
                     ASCYL"
     gene            complement(628..720)
                     /locus_tag="PLR57_02090"
     tRNA            complement(628..720)
                     /locus_tag="PLR57_02090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Ser,seq:gct)
     gene            complement(739..828)
                     /locus_tag="PLR57_02095"
     tRNA            complement(739..828)
                     /locus_tag="PLR57_02095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(792..794),aa:Ser,seq:tga)
     gene            1088..1480
                     /locus_tag="PLR57_02100"
     CDS             1088..1480
                     /locus_tag="PLR57_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40276.1"
                     /translation="MNMMKKRFFPAALVLLMLVSAGCKQTSSTTLNSEYKIFYGGCQI
                     EATVRELTVDGGTTTARIHFLNLGSETLSSLQALVEFVDAEGNTIDSDVLDLTFNTEI
                     TVGEGFSETATCKSNDKIVNVYVTEYTP"
     gene            complement(1483..2238)
                     /locus_tag="PLR57_02105"
     CDS             complement(1483..2238)
                     /locus_tag="PLR57_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HQQ40277.1"
                     /translation="MEQTPLKRRPVSYKDGVNWNPWHGCHKYSAGCLNCYVYRMDGRH
                     GKDADVVKKTNDFYLPIAHARDGSYKIPSGSLIWTCFTSDFLLEDADEWRSEAWRMMK
                     ERSDCAFLFITKRITRFAGCVPEDWGEGYPNVSICCTMENQAKANERLPVFREAKIRH
                     KAIVCEPLLERIDFGAYLGDWVDGVTVGGESGEQARTCEYDWILDIREQCIRAGVPFH
                     FKQTGANFVKDGKRYAIRRAFQHSQARKANIDT"
     gene            2345..2932
                     /locus_tag="PLR57_02110"
     CDS             2345..2932
                     /locus_tag="PLR57_02110"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HQQ40278.1"
                     /translation="MNELLQAIERFAQGTTIWVYLFIFFGKILEVSFGTLRIVLINRG
                     ERTVGSMIAIIEITLWLVVASSVLTGFREDFLKGIVYAVAFACGNYIGSWLDELFAFG
                     LSSMQVVLPNMEAAKAAEECLRAKGFGLTSLDVHGRDDDRAMLMMTMQRKRLSEALTL
                     VEHSCPGAVVTVTDIKTQRGGYLERAVRKQLRNGK"
     gene            3146..>3404
                     /locus_tag="PLR57_02115"
     CDS             3146..>3404
                     /locus_tag="PLR57_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HQQ40279.1"
                     /translation="MKKNLLYAFKRTIPILAGFFPLGLAYGILMMKAGYNFLWTGFTC
                     VTVLAGSLQFLMLDFLASGVPIGTIIVMALLLNSRHIFYGLS"
BASE COUNT          780 a          909 c          855 g          860 t
ORIGIN      
        1 agatagcagc tcgccttttg cgcgtccatt tccgcaagaa acgcgcgaag catctttgca
       61 ccgtgccctt tcccctgctc ctctggatcg accgcaagca tcatcacgta ccaggcgtcc
      121 atagcatgca cggctttgcg aatctccaga ctgtggattt cgtacttgtc aagcctgccg
      181 agcagaccgc ggcaaacggt aagcggcatc ttccagccgc cgcgcagaag aaactccatc
      241 ggcggaacgc tgtccgtatt cggcggaagc agcccgatca cgccttcgat attctcgctg
      301 ggcgcaaaaa agtcgtagta cggaaaaccc gtctgaaaga tgacgtcgaa ataccttttc
      361 agccgccggt agctcggtat ctttccgcca aattgataga tcagcgcggg atccgttata
      421 aacgcgcgcg cggaaacctc cgcgcatttc gcgaggtctt tcttttgaat gcggtacagg
      481 ccctgcagtt ccatgaaatg ttctccaatg attaatgtgt ttcggcagta cattgccttc
      541 ttgttttctc tattataatg agcgcacaaa ctgtatacaa gcggcggata caaaagaaaa
      601 ccgcccgaac ggttcattcg ggcggcttgg cggagaaggt gggatttgaa cccacgcacc
      661 ggttacccca gtctactccc ttagcagggg agccccttac agccacttgg gtacttctcc
      721 gtatgcggtt ttcaataatg gcggagagag agggatttga acccccggtg cctttggggc
      781 atcaccagtt ttcaagactg gctccataaa ccactcggac atctctcctt aaacgcaccg
      841 ttagcagtat agcaaattca tggcttgatt gtcaacgatt gaatcttgtc ctgcgttgct
      901 gaaatttgcg gactgtgaat aacagaattt ttcgttacaa aaaccatctt gtcccgctgt
      961 tcacgcagtg tttgccttgc gttcataaag aattgtgcgg ttgcgattgc aagcgtgagc
     1021 ggcatttcct ataataacag tagaatcaca aaaagaaaat attgtttgtt taccaggggg
     1081 taatcctatg aatatgatga aaaaaagatt cttccctgcc gcgctggttt tgctgatgct
     1141 ggtttcggca ggctgcaagc agacttcttc caccacgctc aattccgagt ataaaatttt
     1201 ctacggcggg tgccagattg aagcgaccgt tcgtgaactc accgttgacg gcggaacgac
     1261 caccgcgcga attcattttc ttaaccttgg cagcgaaaca ctttcgtctc tccaggcgct
     1321 ggtcgaattc gtggatgccg aaggcaacac gattgatagc gacgtgctcg acctgacgtt
     1381 taacacggag attaccgtcg gcgaaggctt tagcgaaact gcaacctgca agtccaacga
     1441 caaaattgtg aatgtctatg ttacggaata tacgccgtaa cgtcaggtat cgatgttcgc
     1501 ctttctcgcc tgcgaatgct gaaacgcacg gcggatggcg taccgctttc cgtccttgac
     1561 gaaattagcg cccgtctgct taaagtgaaa cggaacgcca gcgcggatgc actgctcgcg
     1621 aatatcgaga atccagtcgt attcgcaggt gcgcgcctgt tcgccggact ctcctccaac
     1681 ggttacaccg tccacccaat cgccaaggta tgcgccaaag tcgattcgtt ccagcaaagg
     1741 ctcgcagacg attgccttat gccgaatctt tgcctcccga aacaccggaa ggcgttcgtt
     1801 cgctttcgct tgattctcca ttgtgcagca aattgaaacg ttcgggtagc cctctcccca
     1861 atcctccgga acgcatccgg caaaccgcgt tatccgcttt gttataaaca gaaacgcgca
     1921 gtcggaacgc tctttcatca tccgccaggc ttcgcttctc cactcatccg cgtcttccag
     1981 cagaaagtcc gatgtaaaac aggtccatat cagcgacccg gagggtattt tataggaacc
     2041 gtcccgcgcg tgcgcaatcg gcaggtagaa gtcgttggtc tttttcacga cgtccgcgtc
     2101 cttgccgtgc cggccgtcca tgcggtagac atagcagttc aaacacccgg cggaatactt
     2161 atggcatccg tgccaggggt tccagttgac gccgtcttta taagaaaccg gccttctttt
     2221 gagtggagtc tgttccatat ctgttccatc cgggcgtttt acgcccacaa ttcgtcacaa
     2281 ttcgtttcgt tgacattgcc gacacgggta cagtataatc ggcaggctga aaggagcgca
     2341 tactatgaat gaattattac aggcaatcga gcggttcgcc caagggacga caatctgggt
     2401 ttacctgttc attttctttg gaaaaatcct tgaggtatct ttcggtacgc ttcgtatcgt
     2461 gctgattaac cgcggtgagc gcaccgttgg atccatgatt gcaatcattg aaattacgct
     2521 ctggcttgtc gttgcgagca gtgttctgac gggctttcgg gaagattttc tcaagggcat
     2581 cgtctatgcc gttgcgtttg cctgcggcaa ttatatcggc tcctggcttg acgagttgtt
     2641 cgcgtttggt ctgagctcca tgcaggttgt actgcccaat atggaagccg cgaaagccgc
     2701 ggaagagtgc ctgcgcgcaa aaggcttcgg cctgacctcg ctggacgttc acggacgcga
     2761 cgacgaccgt gcgatgctga tgatgaccat gcagcgcaag cgtctttccg aagcgcttac
     2821 tcttgtggaa cacagctgcc ccggcgcagt cgttaccgtt accgacatta aaacgcagcg
     2881 cggcggctat ctggagcgcg ccgttcgaaa gcagctccgc aacggcaaat aataggctct
     2941 atcgtttgat taaaaggcgt cgttaacagc ggcgcttttt ctgttttggg gaagattgtg
     3001 ttttcgcata cgccaatatt gacaggcgca tctgcagtta ctataatcga acgaaacgcg
     3061 cgcgagccgt caatctttac tctttcaatc gtagcatcga tcaaaccgac cgtcaataaa
     3121 gccgaaagca ctcgagggaa acagcgtgaa aaagaatctt ctctatgcgt tcaaacgaac
     3181 aatcccgatt ttggcgggat tctttccgct tgggttggct tacggcatcc tcatgatgaa
     3241 ggcaggctac aacttccttt ggacggggtt tacatgcgta accgtactgg cgggatcgct
     3301 tcagtttttg atgctggact ttcttgccag cggcgtaccg attggcacaa ttattgtgat
     3361 ggcgctcttg ctcaacagca gacatatctt ctacgggctg tcgt
//
LOCUS       DAOMGY010000088         3400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_29494_length_3400_cov_3.518386, whole genome shotgun sequence.
ACCESSION   DAOMGY010000088 DAOMGY010000000
VERSION     DAOMGY010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29494_length_3400_cov_3.518386"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..1466
                     /locus_tag="PLR57_02120"
     CDS             312..1466
                     /locus_tag="PLR57_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQQ40280.1"
                     /translation="MKMNQTEPVHAESAAPAVEPTQTEAKKPKKRWRILSSVFLFALI
                     AGAMYFALRDTSLAEVWSAVQSANPLWLLCALGASLIAAVMFGIALHISLRALDGKRI
                     SLIRSIGFGFIGQYYTAITPAGAAGQPMQLYYMLGYGVEVSHASLSLLLVNASHQIVV
                     LLIPALLFPFRASLILNNLGAFLWFLLFGAIVNIGLILFLLFAMFSQSFVNRAVNWAI
                     RLMTKIKIIKRPEAIQARVNKQIALYKQGAEVFRKHPWLLVGELFAYVVLLGMQFVIP
                     YFIYRAFGLSGFGMVDFIALQSILYLAVCFLPLPGSAGASESGFVTIFRVLFQSSLIV
                     PAMLLSRLMSFYVILLLSGVISLTMHLILTNRRKRALAAAVELPEPPQES"
     gene            complement(1524..2168)
                     /locus_tag="PLR57_02125"
     CDS             complement(1524..2168)
                     /locus_tag="PLR57_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HQQ40281.1"
                     /translation="MQNLQRHGSGTHAAEYDSRLVHLHTPRTPAIRAGIVDELTRSPA
                     GRGAGRVRKNIKKTLKIPAGIDNGQTLRLPNMGSAGHRGGPNGDLYVTIGVRSHKQFV
                     RKGFDLYLEIPISYTMAALGGSITVPTLRESVKYTIPAGTQPGSTFRLREQGIKHLNA
                     NGTGDLLVTVKVEVPKHLTEEQKSLLEQYAATLGESGDGRTGKKNVFNRSSNRK"
     gene            complement(2534..2677)
                     /locus_tag="PLR57_02130"
                     /pseudo
     CDS             complement(2534..2677)
                     /locus_tag="PLR57_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            complement(2829..>3400)
                     /locus_tag="PLR57_02135"
     CDS             complement(2829..>3400)
                     /locus_tag="PLR57_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQQ40282.1"
                     /translation="KFQLTGIAPAPRGMPQIAVSFDIDANGIVHVSAKDLGTGNEQQV
                     TITASSNLNEDEIKRAVSDAEKFAGEDKKRKEEVETRNHADQLIYTTERSLKEMGDKI
                     DAAGKEEIEKAVADLRKTLESGDVGLIKTASEHLTEVSYRVFGKVYEQAAQQQQAAGG
                     TQDGPQDGAQGGAQDDVVDADYEVVDDNK"
BASE COUNT          578 a         1034 c          913 g          875 t
ORIGIN      
        1 tccccggcta ctataccgac aaaacgcact ggagttcgct ctttttagac acgctcgtaa
       61 cggacgatac cgcaaaatcc atgctggaca acgcgtatct gaccacgctc gcaggcctta
      121 ctaaaaaact gcgcgcgcaa atactcagcg agtagccgtt tcccgaataa tccgctttag
      181 catccgaaat tcaccaaaac gacgcgccat ttgcatctgg cgcgccgttt tctttgcgtt
      241 catatttttt tcacgtttgc tctcaggcgc gcatatagct ttttgtcggt ttctttagta
      301 tgatacaact catgaagatg aatcaaacag aacccgttca cgctgagagc gccgctcccg
      361 ccgtggaacc gacgcaaacc gaagcaaaaa agccgaagaa acgctggcgc atactgagtt
      421 ccgtttttct gttcgcgctg atcgcgggag ccatgtattt tgcgctgcgc gatacttccc
      481 ttgcggaagt ctggagcgcc gtccagtcag ccaatccgct ctggctgctc tgtgcgctgg
      541 gcgcgtcgct cattgccgcc gttatgttcg gaatcgcgct gcatatttcg ctgcgcgcgc
      601 tcgacggaaa acgcatttca ctgatccgca gtatcgggtt cggatttatc ggccagtact
      661 atacggccat cacgcccgct ggcgcagcag gccagccgat gcagctttac tacatgctgg
      721 gatacggcgt ggaagtttca cacgcttcgc tttcactgct gctggtcaac gcgtcgcatc
      781 aaatcgtcgt gctgctgatt cccgcgctgc tgtttccgtt tcgcgcaagc ctgattctca
      841 ataacctcgg agcgtttctc tggtttctgc tctttggcgc aatcgtcaat atcgggctca
      901 tcctgtttct gttgtttgca atgttctcgc agagctttgt taaccgcgcc gtcaactggg
      961 cgattcgtct tatgaccaaa atcaaaatta taaagcgccc ggaggccatt caggcgcgcg
     1021 tcaacaagca gattgccctt tacaagcagg gtgccgaggt gtttcgtaag cacccgtggc
     1081 ttttggtcgg cgagctgttt gcgtatgtcg ttctgctcgg catgcagttt gtaataccat
     1141 actttatcta ccgtgcgttc ggcctcagcg gattcggcat ggtcgacttc atcgccctgc
     1201 agtcgattct ctacctcgcg gtctgctttt tgccgctacc cggctccgca ggcgcaagcg
     1261 agagcgggtt tgtaacgatc ttccgcgtgc tgttccagag ttctcttatc gtgcccgcca
     1321 tgctgctctc gcgcttgatg agcttttacg tcatcctgct cctcagcggc gtgatatcgc
     1381 tcacgatgca cctcatcctc acaaacaggc gcaaacgcgc tcttgccgcc gcagttgaac
     1441 tccccgagcc gccgcaggaa tcttaatcaa acgcgtttat gtatgaaaaa agcctttccg
     1501 actggaaagg ctttttggtt acgttatttt ctgttgcttg agcggttgaa cacgtttttc
     1561 ttgccggttc tgccgtctcc gctttcaccc agcgttgcgg cgtactgttc cagcagcgac
     1621 ttctgctctt ccgtcaggtg ctttggcact tccaccttca cggtgaccag caggtcgccc
     1681 gtgccgtttg cgttgaggtg cttaatgccc tgttcgcgca ggcggaaggt cgaacccggc
     1741 tgcgttccgg cggggatggt gtactttacg ctttctctga gcgtcggcac agtgatgctt
     1801 ccgccgagcg ccgccatggt gtacgaaatc ggaatctcca aatacagatc gaaccctttt
     1861 cgcacaaact gcttatgtga gcgcacgccg atggtcacgt atagatcgcc gttcgggccg
     1921 ccgcggtgac ccgcgctgcc catgttcggc aggcgcagcg tttgcccgtt gtcgatgccc
     1981 gcgggaatct tgagcgtctt tttgatgttc ttgcggacgc gccccgcacc ccggcctgcc
     2041 ggggaacggg taagttcgtc aacgatccct gcacggattg ccggggtgcg gggcgtgtgg
     2101 aggtgaacga gccgagaatc gtattctgct gcgtgcgtac ctgacccgtg ccgttgcagg
     2161 ttttgcactt tacgggttcc gtgcccggct tcgcgccgct gccgccgcag gtttcgcaga
     2221 cttcttcacg cggaatcgta atctcctttt ccgcgccgaa cgcggcttcc tcaaaggaaa
     2281 tcgtgatgct gtagcgcagg tcctctcccg gtacggggcc gttgcgggtg gaaccgccga
     2341 atccgccgcc ggaaaacgcg tcgccgaaca tctgccccag aatatcgcca aggtcgccaa
     2401 acccgccgaa accgccgccc gcgaacgggt cctgcccgcc catggagggg tcgaacgccg
     2461 catggccgta ttgatcgtac ttggcgcgct tttcggggtc gacgagcact tccgccgctt
     2521 cgttgagttc cttgaattcc gcttccgcct ttttatcgcc cggatgcagg tcggggtggc
     2581 attcctttgc acgtttacgg aacgcgctct tgatctcctc taccgttgcg gttttggaaa
     2641 cgcccagtat ttcgtaataa tcgcgtttgt cagccaatgc cccttacctc tcgataagga
     2701 ggcgcatagc attctgcgcc tccttatttc ccttcgtttt ctattaacgc ccaagacggg
     2761 cgtatcattc tcttctgttc gagggagaac gaaagtctag ctttcgtctc ccccgatagt
     2821 ataccagatt atttgttgtc gtccacaacc tcgtagtcgg cgtccacaac gtcgtcctgc
     2881 gcgccgccct gagcgccgtc ctgcggtccg tcctgcgttc cgcctgccgc ctgctgctgc
     2941 tgcgcggcct gctcgtagac tttgccgaat acgcggtagc tcacctcggt gaggtgttcg
     3001 ctcgcggtct tgatgaggcc aacgtcgccg ctttcaagcg tctttctgag gtcggcaacc
     3061 gccttttcaa tctcttcctt gccggcagcg tcgatcttat cgcccatttc cttgagggag
     3121 cgctcggtgg tgtagatcag ctggtcggca tggttgcggg tttcaacctc ttccttgcgc
     3181 ttcttgtctt cgcccgcgaa cttttcggcg tccgaaaccg cgcgcttgat ctcatcctcg
     3241 ttgaggttgc tcgaagcggt aatggtcacc tgctgttcgt tgcccgtgcc aaggtccttg
     3301 gcggaaacgt gcacgatgcc gtttgcgtcg atatcgaacg agaccgcgat ctgcggcatg
     3361 ccgcgcggcg cgggcgcgat gcccgtgagc tggaacttgc
//
LOCUS       DAOMGY010000089         3397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_29541_length_3397_cov_2.977558, whole genome shotgun sequence.
ACCESSION   DAOMGY010000089 DAOMGY010000000
VERSION     DAOMGY010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29541_length_3397_cov_2.977558"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(321..716)
                     /locus_tag="PLR57_02140"
     CDS             complement(321..716)
                     /locus_tag="PLR57_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40283.1"
                     /translation="MSYISEKVAYLDGLADGLSLKEEGTDKLLRGIIDVLGAVAETLE
                     EQDETIDDLSDCIDGIYDELDELDGGFDEDDFIEVTCPHCGETVYFDEEMIDSEDGLI
                     CPNCNGTIEFEIPGCECGCGCGCEDEDKD"
     gene            complement(780..1337)
                     /gene="efp"
                     /locus_tag="PLR57_02145"
     CDS             complement(780..1337)
                     /gene="efp"
                     /locus_tag="PLR57_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQQ40284.1"
                     /translation="MISAGEFRKGMTVEIDGSVWSISDFQHVKPGKGAAFVRTRLKNV
                     MTGAVLERTFNPTDKYPPAHIETKEMQYLYEDGGLYYFMDVETYEQLPLNHDQVEDAI
                     DFIVENQNVKVRFFKGAPFSVEAPNFVELTITHSEPGVRGDTATGTTKPATLETGYKL
                     NVPLFVNEGDRIRVDTRTGEYMERV"
     gene            complement(1515..2561)
                     /locus_tag="PLR57_02150"
     CDS             complement(1515..2561)
                     /locus_tag="PLR57_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQQ40285.1"
                     /translation="MRENLRKNGLDAALITSGVNIRYYSGFTSPDAVLVVTQKSAYLV
                     TDFRYTIQAKAQTMGCAEVVETTYAEQLGRIRSFFEQDGAKRAGFEQNHLSVGAFAEY
                     KTFPLEWVEFHDEITRPRLHKSAEEVASLQAAQKIADMSYAEFLGRVRPGMTELEATA
                     ELIYVCSKNGSEGPAFDPIVAGGANGAMCHAVPGARKLQKGDLVVVDFGCVSDGYRSD
                     MTRTFGIGSIGAEEKKICEIVKTAQQMALDALVNGVSGKALDAVARDYIASFGYGAAF
                     GHGLGHGFGLEIHEPPRAAMSSTDTLTAGMTITVEPGIYVEGLCGVRIEDCCVVTETG
                     HLNLVSSPKELVLI"
     gene            complement(2719..>3397)
                     /gene="mltG"
                     /locus_tag="PLR57_02155"
     CDS             complement(2719..>3397)
                     /gene="mltG"
                     /locus_tag="PLR57_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQQ40286.1"
                     /translation="KGLISNEADLLEAARTGSAFTNFAFINDVASMSNVGSRAYVLEG
                     YLFPDTYEVYADASVETILIKMLNRFNEVFTDEYLTRAQQLNMTMDQVLTLASLIERE
                     AQAPGDFAKVSAVFHNRLSQDMPLQSCASLSYVLGVTKYVFNEDERATDSLYNTYKYN
                     GLPVGPVCNPGKAAIEAALYPNEEYLAGGYLYFCNQNPAETSELVFAKTYEEHQANVE
                     KYQQYWG"
BASE COUNT          689 a          980 c          907 g          821 t
ORIGIN      
        1 ctaaaaaaaa cgcgtccata ttaaacgtcc ctctgttctc aaaaaagatt cgccgttcgc
       61 aagaacaata gacggcgacg ctttgatcta agggtacaaa ttcgccgttt actaaacatt
      121 attcggcgac gctttgctct aagggtactt attcgccgtt tatataagaa ctccttgttt
      181 tgtgagcgga aatgacagaa tcttacgcgc agttttcact tttgacgcaa ttggcaggtg
      241 atccggcgcg cgtttccgcc aacagcaaaa aaacccgccc gcagtttttc tgcaaacggg
      301 gtttttatat tgctaaaccg ttagtcttta tcctcgtcct cgcagccgca gccacagccg
      361 cattcgcagc cggggatttc gaactcgatg gttccattgc agttcgggca gatgaggccg
      421 tcctcgctgt cgatcatctc ttcatcaaag tagaccgttt cgccgcagtg cgggcaggtc
      481 acttcgatga aatcgtcttc gtcgaatccg ccgtcgagct cgtccagctc gtcgtagatg
      541 ccgtcgatgc agtccgaaag atcgtcaatg gtttcgtcct gctcttccag cgtttccgca
      601 accgcgccca gcacgtcgat aatgccgcgc agcagtttgt cggttccttc ttccttcagc
      661 gaaaggccgt cggccagacc gtcgagatag gcaacttttt cagaaatata actcatgggt
      721 attccctcct gccgacccca aacgagcggc ttttgattca taattggtgg tagcttgctc
      781 tagacgcgct ccatgtattc gcccgtacgg gtgtccacgc ggatgcggtc gccttcgttt
      841 acgaacaacg gaacgttgag cttgtagccg gtttcgagcg tcgcgggctt ggtcgtgccg
      901 gtcgcggtat cgccgcgcac gccgggctcg gagtgcgtaa tggtcagttc aacgaagttc
      961 ggcgcttcaa ccgagaacgg cgcgcccttg aagaagcgga ctttcacgtt ctggttttcc
     1021 acgatgaaat cgatggcgtc ttccacctgg tcgtggttga gcggcagctg ctcgtaggtt
     1081 tccacatcca tgaagtaata cagcccgccg tcttcgtaga ggtactgcat ttccttggtc
     1141 tcgatgtgcg ccggcgggta tttgtccgtc gggttaaacg tgcgctcgag caccgcgccg
     1201 gtcatcacgt tcttaaggcg agtacgcacg aaagccgcgc ccttgccggg ttttacatgc
     1261 tggaagtccg agatcgacca gaccgacccg tcaatttcca cggtcatgcc ttttctgaat
     1321 tcgcctgcac tgatcataaa tttgattcct ccgtaacgtt cgataaaaat cgaagtttga
     1381 tagtatggac tttgtatgac gcatcgccgt ttgattgcgg cgagttgttt cggtgaaagt
     1441 aactaaatga gcacgagctc tgatcgaatg gtctgttgca tacagagtca ttcggcttta
     1501 ttcagcaacc aatactagat gagcacgagc tccttgggtg aggaaacgag attgaggtgc
     1561 cccgtttcgg tcaccacgca gcagtcctca atacgaacgc cgcacaggcc ctccacatag
     1621 atgcccggct cgaccgtgat ggtcatgccc gcagtcagcg tgtccgtgct gctcatggcg
     1681 gcgcgcggcg gctcgtgaat ttccagcccg aagccgtggc caaggccgtg gccaaacgcc
     1741 gcgccgtagc cgaagcttgc gatgtaatcc cgcgctaccg cgtccagcgc tttaccgctg
     1801 acgccgttaa ccagcgcgtc cagcgccatc tgctgagcgg tcttgacgat ttcgcaaatc
     1861 ttcttttctt ccgcgccgat ggagccgatg ccgaacgtgc gcgtcatatc cgagcggtaa
     1921 ccgtcgctca cgcagccaaa gtccacaacc acgaggtctc ccttttggag cttgcgcgcg
     1981 ccgggtaccg cgtggcacat ggcgccgttt gcgccgcctg caacgatggg atcgaacgcg
     2041 gggccttcgc tgccgttttt ggagcagacg tagatgagct ccgccgtcgc ttccagctcg
     2101 gtcatgccgg gacggacgcg gccgaggaac tcggcgtagg acatatcggc aatcttctgg
     2161 gcagcctgca ggctcgccac ttcttccgca gatttatgca gacgcgggcg cgtgatttcg
     2221 tcgtggaact cgacccactc cagcggaaag gtcttgtact cggcaaacgc gccaacgctg
     2281 aggtggttct gttcgaaccc ggcgcgtttt gcgccgtcct gctcaaagaa gctgcgaatg
     2341 cgcccgagct gttcggcata cgtggtttcc acaacctccg cgcagcccat tgtctgcgcc
     2401 ttggcctgaa tggtataccg aaaatcggtc acgagatacg cgctcttttg cgttaccacg
     2461 agcacagcgt ccggactggt aaatcccgaa taatagcgga tattcacgcc gctggtgatc
     2521 agcgccgcgt ccagaccgtt tttgcgcaga ttttcgcgca atccttctaa tctctttttc
     2581 atacggagtt attttaccac agttgttccc gccgcacaac aaaaaagcgg cagagctacc
     2641 tttcttttta ctggaagcac aggtcttccc gcaataaagc tccggctatg ttcagccgga
     2701 gttttataaa tcaaatcgtt atccccaata ctgttgatac ttctccacgt ttgcctgatg
     2761 ctcttcgtat gtcttggcaa acacaagctc gctggtttcg gcgggattct ggttgcagaa
     2821 gtagaggtag ccgcccgcga ggtattcctc attgggatac agcgccgcct cgatggccgc
     2881 cttgccgggg ttgcaaacgg ggccgacggg caggccgttg tatttgtagg tattgtagag
     2941 cgagtcggtc gcgcgctcgt cctcgttgaa cacgtacttg gttacgccca gcacatacga
     3001 aaggctcgcg cagctctgca gcggcatatc ctgactcagt cggttatgga acaccgcaga
     3061 aaccttggca aagtcgcccg gcgcctgcgc ctcgcgttca atgagcgaag caagcgtcag
     3121 cacctgatcc atggtcatgt tgagctgctg cgcacgggtc agatactcgt ccgtaaacac
     3181 ctcgttaaag cggttgagca ttttaatgag gatcgtttcc acgctcgcgt cggcatacac
     3241 ctcatacgta tccgggaaga ggtacccttc cagcacatac gcgcggctgc caacgttgct
     3301 cattgacgca acgtcgttga tgaacgcaaa gttcgtaaac gcgctgccgg ttctggcagc
     3361 ctctaataga tcggcttcgt tcgatatcag gcccttt
//
LOCUS       DAOMGY010000090         3386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_29663_length_3386_cov_3.128190, whole genome shotgun sequence.
ACCESSION   DAOMGY010000090 DAOMGY010000000
VERSION     DAOMGY010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29663_length_3386_cov_3.128190"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1061
                     /gene="ispG"
                     /locus_tag="PLR57_02160"
                     /gene_synonym="gcpE"
     CDS             3..1061
                     /gene="ispG"
                     /locus_tag="PLR57_02160"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQQ40287.1"
                     /translation="MTRQIFVGGVPVGGGAPVTVQSMTNTDTANAEATAAQINALTAA
                     GCEIVRSSVYNMACAKALRDIKSKISIPLVADIHFDYRLAIAAIENGADKLRFNPGNI
                     GGEDRVRSLVACAKEHRVPIRIGVNAGSLEAELKQKHAGNTVAAMVESALRHVKILER
                     EGFEEIVLSLKASDVRTTVEAYREISRIVDYPLHVGVTETGDVQSGIIKSAAGIGALL
                     LEGIGDTIRVSLTDDPVREVEAGLKILRAVGLRKDDIELVSCPTCGRTRVDVMKMVEL
                     VNSRLPHHQGYLKVAVMGCAVNGPGEASDADIGVAFGNGNGVLFVRGEQVYHGSAEQV
                     VEALIERATTMLEEQKKA"
     gene            1120..>3386
                     /locus_tag="PLR57_02165"
     CDS             1120..>3386
                     /locus_tag="PLR57_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HQQ40288.1"
                     /translation="MEEELVSTLRSVGIDDASVTLDHGTLNREQNLLTVFFRCEREFS
                     ISVSQKLAAALEEKSGGARVKVVWLAEKPYEPETSAPAVTTASAESSAPTPNSAAAPV
                     KPKQVVGKRVLIGAYIASALKRRIRDVAEGKNKIVIEGTVVAIAERNPFKNKRKKDQT
                     CYPLDVSVSDGTDTIYCSLLLDTPEQLAKIVGEFEKAKGKGDHLLVQGVAKTSKITGE
                     LMLYANDVCLIPFEGRKDECELKRVELHLHTRMSAQDGITDVDKAFETAKRFGHRALA
                     VTDHGVVQAFPAEAKAAKKSGVKPIFGVEGYLQRESEMADMDQTFVVFDLETTGLKPE
                     QCEIIEVGAVKIKNGEVIDRFQTFVNDGGIVPPRITELTGITTEMLYGASNSRQVLGD
                     FYEFSKGCCLVAHNAEFDMGFIRHHGERFGIKFDQQYSDTLMLSRYLVRDLENYKLDT
                     VCEALNVVLKDHHRASDDANATGEVFLHLLERLRALGLTSLPVRTDATRPEGGKKRAK
                     TNHIVILARTQAGLKNLYRIVSFAHLEHFHYHPVIPYSLLSIFRDGLILGSACADGEL
                     YQAILHDKGAESIKSIARFYDYLEIQPRCNNAFLVRAGEATDERILEINREIVSLGER
                     LGIPVAATGDVHFLNPEDAIYREVILTKNGFEDASLQPPLYFKTTTEMLEEFSYLGAE
                     TAKRVVVDVPNEIADRCEQIKPFPDGTHAPTIENASEILQEMAQKRAHELYGDPLPPV
                     VQARLDRELKSIIGNG"
BASE COUNT          783 a          892 c         1022 g          689 t
ORIGIN      
        1 aactgacacg tcaaatcttc gtcggcgggg tccccgtcgg cggaggcgcg ccggtaacgg
       61 tgcagtccat gacgaatacg gataccgcga acgccgaagc cactgcggcg cagatcaacg
      121 cgcttactgc cgcgggctgc gaaattgtgc gctcgtcggt ctataacatg gcgtgcgcaa
      181 aagctctgcg tgatattaaa agcaagattt ccattccgct ggttgcggat attcatttcg
      241 actaccggct cgccattgcg gcaattgaaa atggcgcgga taagctgcgc ttcaatcccg
      301 gcaacatcgg cggggaagac cgcgttcgct cgctggtcgc ctgcgcaaag gaacaccgcg
      361 ttcccatccg catcggggtc aacgcaggtt cgctggaagc ggaactcaag cagaagcacg
      421 caggcaacac ggttgcggca atggtggaaa gcgcgctgcg gcatgtaaag attctggagc
      481 gggaaggctt tgaagagatc gtgctttcgt taaaggcttc ggatgtgcgc accactgtgg
      541 aagcgtaccg tgagatttcg cgcattgttg attatccgct gcacgttggc gtaacggaaa
      601 cgggcgatgt gcagagcgga atcatcaaat ccgccgcggg catcggcgcg ctgctgctgg
      661 aaggaatcgg ggatacgatt cgcgtttctc tgacggacga cccggtacgc gaagtcgaag
      721 ctgggctcaa gattttaaga gcggtcggcc tcagaaaaga cgatattgaa cttgtgagct
      781 gcccgacttg cggcagaacc cgcgtggacg tgatgaagat ggtcgagctg gtcaacagcc
      841 gcctgccgca tcatcagggc tatttgaaag tcgcggtcat gggctgcgcg gtcaacggcc
      901 caggcgaagc gagcgacgcg gatatcggcg ttgcgtttgg aaacggaaac ggcgtgctgt
      961 ttgtccgcgg cgagcaggtg tatcacggtt cagcagagca ggttgtggaa gctttaatcg
     1021 agcgcgcgac aacgatgctg gaggagcaga agaaggctta aataacatca tattccggtt
     1081 ggaagccggg ggtcatctct tgggaaaggg actgggacgt tggaagagga actggtatca
     1141 acgctgcgga gcgtcggcat tgacgatgct tccgtaacgc tggatcatgg gacgctgaac
     1201 cgtgagcaaa acctgctgac ggtctttttt cgttgcgagc gggaattttc catttccgtt
     1261 tcgcaaaaac tcgccgccgc actggaagaa aagtccggtg gcgcgcgcgt aaaggtcgtg
     1321 tggctcgcgg agaagcccta cgagccggaa acttccgcac ctgcggtaac aactgcttcc
     1381 gctgaatctt ccgcacccac accgaacagc gcggcggctc cggtcaagcc caagcaggtt
     1441 gtcggcaagc gcgtgctgat tggcgcatac atcgcttccg cgctgaagcg gcgcattcgt
     1501 gacgttgcgg aaggcaagaa taaaatcgtc atcgaaggca cggttgtcgc cattgcggaa
     1561 cggaatccgt tcaaaaacaa gcgaaaaaag gatcaaacct gttacccgct ggatgtatcc
     1621 gtatccgacg gaacggatac tatctattgt tcgctcctgc tcgatacgcc ggaacagctc
     1681 gccaagatcg ttggcgagtt tgagaaggcg aaaggcaaag gagaccacct gcttgtgcag
     1741 ggcgtggcaa aaacttccaa aatcacaggc gaactgatgc tttacgcaaa cgacgtatgc
     1801 ctgattccgt ttgaagggcg caaggatgag tgcgaactca agcgcgtaga actgcatctg
     1861 catacgcgta tgtctgcaca ggacggcatt acggacgttg acaaggcgtt tgaaacggca
     1921 aagcggttcg ggcatagagc gctcgcggtc accgaccacg gcgtggtgca ggcgtttccg
     1981 gcggaagcca aggccgcaaa gaagtccggc gtcaaaccga ttttcggcgt ggaaggctac
     2041 ctccagcgcg aatcggaaat ggcggatatg gatcaaacct tcgtcgtgtt cgacttggaa
     2101 acaacaggcc tcaagccgga acagtgcgaa attatcgaag tcggcgcggt gaagatcaag
     2161 aacggggaag tgatagaccg ctttcagacg ttcgtcaacg acggcggcat cgttccgccg
     2221 cgtatcaccg aactcaccgg catcacgacg gaaatgctct atggcgcgtc gaattcccgt
     2281 caggtattgg gcgattttta tgaattctcg aaggggtgct gcctggttgc gcacaacgcg
     2341 gaattcgaca tggggttcat ccgccatcac ggcgagcggt tcggcataaa attcgatcag
     2401 cagtattccg acactctgat gctttctcgc tatctcgtgc gggatttgga aaactacaag
     2461 ctggataccg tctgcgaagc gttgaatgtg gtgctcaaag accatcaccg cgcctcggac
     2521 gacgcaaacg ccacgggcga agtgttcctg catctgctgg agcgtcttcg cgcgctgggg
     2581 ctcacttcgc tgcctgtacg cacggatgcg acccgtccgg aaggcggtaa aaagcgcgcc
     2641 aagacgaacc atatcgtcat tctggcaagg acgcaggcgg gtttgaaaaa cctctaccgc
     2701 atcgtgagct ttgcgcatct tgagcatttt cattatcatc cggttattcc gtacagcctg
     2761 ctttccatct tccgcgacgg attgattctc ggctccgctt gcgcggacgg ggaactgtat
     2821 caggcgattc tgcacgataa aggcgcggaa tcgattaaaa gcatcgcgcg gttctacgac
     2881 tatttggaaa ttcagccgcg ctgcaacaac gcgtttcttg tgcgcgcggg ggaagccacg
     2941 gacgaacgga ttctggagat caaccgtgaa atcgtatctc tgggcgaacg acttggcatc
     3001 ccggttgccg ctaccggcga cgttcacttt ttgaatccgg aggacgcgat ctaccgcgaa
     3061 gttattctta ctaaaaacgg ctttgaggac gcttctttgc agccgccgct gtactttaaa
     3121 acgacgacgg agatgctgga agagttttct taccttggcg cagaaacggc aaaaagggtt
     3181 gttgttgacg ttccgaacga aattgccgac cgctgcgaac agatcaaacc cttcccggat
     3241 ggtacgcacg cgccgaccat tgagaacgca agcgagattt tgcaggagat ggcgcagaag
     3301 cgcgcgcacg agctctatgg cgacccgctc ccgcctgtcg tgcaggctcg tctcgatcgc
     3361 gagctcaagt cgatcatcgg caacgg
//
LOCUS       DAOMGY010000091         3370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_29888_length_3370_cov_3.203318, whole genome shotgun sequence.
ACCESSION   DAOMGY010000091 DAOMGY010000000
VERSION     DAOMGY010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29888_length_3370_cov_3.203318"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLR57_02170"
     CDS             <1..582
                     /locus_tag="PLR57_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQQ40289.1"
                     /translation="PIGAARGMIGIPNYSTLAWILFFMTLTLFLLKNLIYSPYGRAFI
                     AIRDNEIAADVMGINTTKYKITAFCIAAFIAGVAGGLYAHVLAFIQPDSFSFTKSSDF
                     LVFMYAGGSGSLTGSIVGAALLTVLPEVLRFLSDWRIAVYAAVLVIVMLYRSDGLCGG
                     KELPFMRIKRNSLYDKPIFPRKNGKNEPAGSNR"
     gene            604..1383
                     /locus_tag="PLR57_02175"
     CDS             604..1383
                     /locus_tag="PLR57_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40290.1"
                     /translation="MSILEVKNLSIMFGGLCAVDDFNLSLEQGEMVAVIGPNGAGKTT
                     VFNMLTGIYKPTSGTITYEGRNMAGLKPYQFAQAGMTRTFQNIRLFSNASVADNVKMA
                     HTMHNQQSLASCFLRTPAMQRSERELDEEVMALLDELNLADKADFLAKNLPYGEQRRL
                     EVARALMTKPKILLLDEPVAGMITTELEEMARLVVDIRKRFDLTIILIEHHMNFVMPI
                     ADRIKVLDFGKTIAEGLPAEIQKNPRVIEAYLGGEYENGNA"
     gene            1370..2080
                     /locus_tag="PLR57_02180"
     CDS             1370..2080
                     /locus_tag="PLR57_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40291.1"
                     /translation="MAMLEVKDLHVSYGAIKAVQGVSFHLDKGEIVALIGSNGAGKST
                     VLRTISGLEHAKSGSIQFEGRELTKMKAHKIVELGIAHVPEGRRVFSGLTVTENLKMG
                     ANLVKSAAEIRTSFERVFTIFPRLKEREKQKAGTLSGGEQQMLALGRALMTNGKLLML
                     DEPSMGLAPIIVEDIFRIIRQINETGTSILLIEQNAFLALKTASRAYVLETGHISMEG
                     AAKDLLVDERVKSAYLGA"
     gene            2171..3340
                     /locus_tag="PLR57_02185"
     CDS             2171..3340
                     /locus_tag="PLR57_02185"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQQ40292.1"
                     /translation="MELSKLAQHLGGSAIRAMYNEALKMKDTISFTVGEPDFITPKPI
                     IDEACRCWEQGLTHYTPNAGVLPLRQAIAEYHKNDLNPDPETQIMVSCGATEAIQMAL
                     FTLVDPGDEVIVVTPAWPNYFGQIGMTGAILKVVPAYEENNFVPDPEDIKKAIGPKTK
                     VIILNSPSNPTGAVIDKATCKALADILRDSNIYVIADEIYSRLVYDEEGYTSITSFDG
                     MIENTVYISGFSKMFAMTGWRLGYAIARPDIIRNMTKLHENGASCLPAPSQIAAAVGL
                     KQCLPEIETMRKTYLERRNLICSLINSTPGLSIRPPKGAFYAFTNAKELSRATGMTSM
                     ELCMDLLKKTGVVTVPGSGFGDVGEGFIRITYATSTENIKAGFERINGYVRGLGL"
BASE COUNT          763 a          973 c          962 g          672 t
ORIGIN      
        1 cccatcggcg ctgcgcgcgg aatgatcggc attccgaact attcaacgct ggcgtggatt
       61 ctgttcttta tgacgctgac gctgttcttg ctcaaaaacc tgatttattc gccttacggg
      121 cgtgcgttca ttgccattcg tgacaacgaa atcgcggcgg acgtcatggg catcaacacc
      181 accaagtaca aaatcacggc gttctgcatt gccgcgttca tcgcgggcgt tgcgggcggg
      241 ctgtatgccc acgtgctggc gttcattcag ccggactcct tcagctttac caagagctcg
      301 gactttctgg tgtttatgta cgccggcggc agcggttcgc ttacgggttc cattgtaggc
      361 gctgcgcttt tgacggtttt accggaagtc ctgcgcttcc tttcggactg gcgcatcgcc
      421 gtatatgcgg ccgtgctggt catcgttatg ctgtaccgct cggacggact ctgcggcggc
      481 aaggaactgc cgttcatgcg catcaagcgc aattcgctgt atgacaagcc gattttccca
      541 cggaagaacg gcaaaaatga gcccgccggc tcgaaccggt aacggcgaga ggggagagta
      601 ttcatgagca ttcttgaagt aaaaaacctt tcgatcatgt tcggcggtct ctgcgcggtg
      661 gatgatttca acctcagcct cgaacagggc gaaatggttg ccgtcatcgg ccccaacggc
      721 gcgggcaaaa ccaccgtgtt caacatgctg acgggcatct acaagcccac ctccggcacg
      781 attacctacg aaggccgcaa catggcgggg ctcaagcctt accagtttgc gcaggcgggc
      841 atgacccgta ccttccagaa catccggctg ttttccaacg cttcggtggc ggacaacgtc
      901 aagatggcgc acaccatgca caatcagcag agcctcgcga gctgttttct ccgcacgcct
      961 gccatgcagc gcagcgagcg cgaattggat gaggaagtca tggcgctgct ggacgagctg
     1021 aaccttgccg ataaagcgga ttttctcgcc aagaaccttc cttacggcga acagcgccgc
     1081 ctggaggttg cgcgtgcgct gatgaccaag ccgaaaatcc tgctgctcga cgagccggtc
     1141 gcgggcatga tcaccaccga gctggaagag atggcgcgcc ttgtggtgga tatccgcaag
     1201 cgcttcgacc tgaccatcat cctcattgag caccacatga acttcgttat gccgattgcg
     1261 gaccgcatca aggtgctgga cttcggcaag accatcgcgg aagggcttcc ggcggaaatc
     1321 cagaagaacc cccgtgtgat cgaggcttat ttaggaggag aatacgaaaa tggcaatgct
     1381 tgaagtcaag gatctgcacg tatcctacgg tgcgatcaaa gccgttcagg gcgtctcctt
     1441 ccatttggac aagggcgaaa tcgtagcgct tatcggttcc aacggcgcgg gcaaatctac
     1501 ggttctgcgc accatctccg gcctggaaca cgcaaaaagc ggttcgattc agtttgaagg
     1561 ccgggaactg acaaagatga aggcgcataa aatcgtcgaa ctgggcattg cgcacgtgcc
     1621 ggaaggccgc cgcgtctttt ccgggctgac ggttacggaa aacctcaaga tgggcgcgaa
     1681 tctggttaaa tccgccgcgg aaatccgcac gagcttcgag cgcgtattca ccattttccc
     1741 gcggctgaag gaacgcgaaa agcagaaggc gggcaccctc tccggcggcg aacagcaaat
     1801 gctcgcgctg ggacgcgcgc tgatgaccaa cggcaagctc ctgatgctgg acgaaccgtc
     1861 catggggctc gcgccaatca tcgtggaaga catcttccgc atcatccgcc agatcaacga
     1921 aacgggcacg agcatcctgc tcatcgagca gaacgcgttc ctcgcgctca aaaccgcctc
     1981 ccgcgcgtac gttctggaaa cggggcatat cagcatggaa ggtgcggcga aggatctgct
     2041 cgtggacgag cgtgtcaagt ccgcttacct cggcgcgtaa aaagaacaca cggcggaatg
     2101 gaaccgggat cgccccgggg gttgtttccg cacggaagat cagtggaaaa taaatagagg
     2161 atacacgaac atggagttat caaaattagc gcagcatctt ggaggctccg ccatccgcgc
     2221 gatgtataac gaagccctga agatgaagga taccatcagt tttaccgtcg gcgagccgga
     2281 cttcattacg ccgaagccca tcatcgacga agcctgccgc tgctgggagc aggggctgac
     2341 gcactatacc ccgaacgcgg gcgttctgcc gctgaggcag gcgattgcgg aataccataa
     2401 aaacgacctc aaccccgacc cggaaacgca gattatggtt tcctgcgggg cgacggaagc
     2461 catccagatg gcgctgttta cgctggtcga tcccggcgac gaggtcatcg tcgtaacccc
     2521 cgcatggccg aactactttg gccagatcgg catgaccggc gcgattctca aggtcgttcc
     2581 ggcgtacgaa gagaacaact ttgtgcctga ccccgaggat atcaaaaagg cgatcggccc
     2641 caagacgaag gtcatcatcc ttaactcgcc ttccaacccc acgggcgcgg tcatcgacaa
     2701 ggcgacctgc aaagccctgg cggatatttt gcgcgacagt aatatctacg tcattgcgga
     2761 cgaaatctac agccgcctcg tctacgacga agaaggctat accagcatca cttccttcga
     2821 cggcatgatt gaaaacaccg tgtatatcag cgggttttcc aagatgtttg ccatgaccgg
     2881 ctggcgtctg ggctatgcca tcgcgcggcc ggacatcatc cgcaatatga ccaagctgca
     2941 cgaaaacggc gcttcctgcc tgcctgcgcc gagccagatt gccgccgcgg tggggctcaa
     3001 gcagtgcctg ccggagattg aaaccatgcg caaaacgtat ctggagcgca ggaatttgat
     3061 ctgctcgctg attaacagca cgcccggctt gtcgattcgc ccgcccaagg gtgcgttcta
     3121 cgcgtttacc aacgccaagg aactttcccg cgcaacgggc atgacatcca tggaactctg
     3181 catggatctg ctcaaaaaga ccggcgtggt tacggtgccc ggcagcgggt ttggcgacgt
     3241 tggtgaaggc tttatccgca ttacctacgc aacctccacg gagaacatta aggccgggtt
     3301 tgaacgcatc aacggctatg tgagggggct gggactatga gcagcatact agaccgcgtt
     3361 tctgctatcg
//
LOCUS       DAOMGY010000092         3364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_29965_length_3364_cov_2.980659, whole genome shotgun sequence.
ACCESSION   DAOMGY010000092 DAOMGY010000000
VERSION     DAOMGY010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29965_length_3364_cov_2.980659"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLR57_02190"
     CDS             complement(<1..631)
                     /locus_tag="PLR57_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ40293.1"
                     /translation="MKLTVLMDNSTYIDQYYLAEPAVSYLIEDGERTILFDAGYSGAF
                     IENAERMKIDLGRVTDIVLSHGHIDHTGGLPAFFERFHQPVRLTAHPSAFLPKQFEGM
                     PAGSPISLCGLPDWVEQRLTAKAVRISEHVLFLGEVPRVYPFEQDRAVGETLASCGCC
                     WESDTLRDDSSLALSVPGGVFTVCGCAHSGACSTVAAAKERTGEDRVAGL"
     gene            complement(672..1265)
                     /locus_tag="PLR57_02195"
     CDS             complement(672..1265)
                     /locus_tag="PLR57_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQQ40294.1"
                     /translation="MIHVLYIDCCIRGELSRTRKLAETFLAELSRTGEYAVERLTLMD
                     EPLVPFQNGFFRQREELLEAGELDHPRFRYAHQFKAAERVVIAAPFWDLSFPALLKVY
                     IENLCVQGITFDCDEKNGTYGVCKAERMLFLTTRGGALEGSPMDNGTKYLSDMAKFFG
                     IPRFGHIAADGLDLGLEPVEAILNRAIERAKEIATDF"
     gene            complement(1297..2226)
                     /locus_tag="PLR57_02200"
     CDS             complement(1297..2226)
                     /locus_tag="PLR57_02200"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HQQ40295.1"
                     /translation="MARILNFGSLNIDHVYRVDAFVRPGETKSSLSLEDHCGGKGLNQ
                     SVAAARAGAAVLHAGLIGRDGNMLLEKLVESGVDCSLLEHSEGVSGHAIIQVDDRGQN
                     CILLHGGTNRMMTEDYVEHALDAFGGRGLVLLQNETNLVGSIIKRAKAKGLMTALNAA
                     PMDEAVFSYPLDLLDWLIVNEVEGAAIARCTREEEILPALERAYPHLNVLLTLGERGS
                     VCVCGGEQTRFGAYRVKAADTTAAGDTFLGYFLNETINGAEVSDALALATAAAALCVQ
                     VMGAADSIPPRERTLSAVRSGELGELLPWGGWR"
     gene            2304..3053
                     /locus_tag="PLR57_02205"
     CDS             2304..3053
                     /locus_tag="PLR57_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HQQ40296.1"
                     /translation="MPQNAPKLQPHLQLDSAHGAVSALLPGDPARLDRIKPFLTDTVE
                     LAYNREFRSLAGTYQGARVLAVSTGIGGASAGIAVEELHNLGIQSMIRIGSCGALQPQ
                     VKLGDLILVSGAVRDDGASKAYIDPIYPAVADAALLAACEKVAQKLGFAFHTGIARSH
                     DSFYTDREEEIDAYWSQKGVLGCDMETAALYVIGRLRQIRCMSILNNVVAYQADTADA
                     IGSYAGGEDAAMRGEEHEIVTALEALASLSA"
     gene            complement(3104..>3364)
                     /locus_tag="PLR57_02210"
     CDS             complement(3104..>3364)
                     /locus_tag="PLR57_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HQQ40297.1"
                     /translation="YSHGYGLPGMCMHDPLALLYADDEALLKTERAGVRVETKGRLTR
                     GKTVTDLYSDKKTEKNAWVATGVDREALKRRLFETMAQYGAE"
BASE COUNT          656 a         1079 c          981 g          648 t
ORIGIN      
        1 aaaggcccgc aacgcggtct tcccccgtcc gctcttttgc cgcggcaacg gtgctgcaag
       61 ccccgctgtg cgcgcagccg cagactgtga atacgccgcc gggtacgctc agcgcaaggg
      121 aagaatcgtc ccgcagcgtg tcgctttccc agcagcagcc gcaggaagcg agcgtttcgc
      181 cgaccgcgcg gtcctgctca aacgggtaga cgcgcggaac ctcgcccaaa aacaagacgt
      241 gctcgctgat tcgaactgcc tttgcagtaa ggcgctgttc aacccagtcc ggcaggccgc
      301 aaagcgaaat gggcgaaccc gcgggcatcc cttcgaactg cttgggcaga aacgcgctgg
      361 ggtgcgccgt gagccttacg ggctggtgaa agcgttcgaa gaacgcgggc aggccgcccg
      421 tatggtcgat atgcccgtgg gagagtacga tatccgtcac gcgtcccaga tcgatcttca
      481 tgcgttccgc gttttcaatg aacgcgccgg aatatcccgc gtcgaacaaa attgtccgct
      541 cgccgtcctc gatgaggtag ctcacggcgg gttcggcgag atagtactga tcgatatagg
      601 tgctgttgtc catgagtacg gtgagcttca tcggttcctc ccgcgcgggt gttccgttcg
      661 tttgactagg attagaaatc ggtcgctatt tctttcgcgc gctcgatggc gcggttcaaa
      721 atcgcctcca cgggctcaag ccccaaatcg agaccgtccg ccgcgatatg gccaaagcgc
      781 ggaatgccga aaaacttcgc catgtcgctt aagtactttg tgccgttgtc catgggcgaa
      841 ccttcgagcg cgccgcctcg ggtcgtcaaa aacagcattc gctccgcctt gcagacgccg
      901 taggtgccgt ttttctcgtc gcagtcgaag gtgatgccct gtacgcagag gttttcgatg
      961 tagaccttca acagcgcggg aaagctcaag tcccaaaacg gagccgcaat cacgacccgc
     1021 tccgccgcct tgaactgatg cgcatagcga aaacgcgggt ggtcgagttc ccccgcctcc
     1081 agcagctctt cccgctggcg gaaaaacccg ttctgaaacg gaaccagcgg ctcgtccatc
     1141 agcgtcagcc gctcaacggc gtactcacca gtgcgtgaaa gctccgcaag aaacgtttcc
     1201 gcgagctttc tggtgcggga cagttcgccg cggatacagc agtcgatgta cagtacgtga
     1261 atcaaaccga accctcccgt ctatggatgc atgatctcag cgccagccgc cccaagggag
     1321 cagttcgccc aattcgccgg agcgcaccgc cgagagcgtc cgttcgcgcg gcggaatgga
     1381 atccgcggcg cccatcacct gcacgcagag cgcggcggcg gcggtcgcca gcgcgagcgc
     1441 gtcggaaacc tccgccccgt tgatcgtttc gttgagaaag taccctaaaa acgtatctcc
     1501 cgccgccgtg gtgtccgccg ctttcacgcg atatgcgcca aagcgcgtct gctcgccgcc
     1561 gcagacgcag accgagccgc gctcgcccag cgtgagcaga acgttcagat gtggatacgc
     1621 ccgttccagc gcgggcagaa tttcttcctc gcgggtacac cgcgcgatgg ccgcgccttc
     1681 cacttcgttg acaatcagcc agtccagcag gtccagcggg tacgaaaaca ctgcctcgtc
     1741 catcggagcc gcgttgagcg ccgtcatgag tccctttgcc ttggctcgct ttataataga
     1801 gccaaccagg ttcgtttcgt tctgcaggag cacgagccct ctgccgccga acgcgtccag
     1861 cgcatgttca acataatctt ccgtcatcat ccggttggtg ccgccgtgga gcagaatgca
     1921 gttctggcca cggtcgtcca cctgaatgat cgcgtggccg gaaacgcctt cgctgtgctc
     1981 cagaagcgag cagtcaacgc cgctctcgac gagcttttcc agcagcatgt tcccgtcccg
     2041 cccgatcagt ccggcatgga gcacggccgc tcccgcgcgc gcggctgcaa cggactggtt
     2101 gagccccttg cccccgcagt ggtcttccag cgacaggctg gacttggttt cgccgggacg
     2161 cacaaacgcg tccacgcgat agacatggtc gatgttgaga gaaccaaagt tgagtattct
     2221 tgccacgtga tccttcctcc ggcatagttt tatggtatga tgaaatcgtt cgatactcta
     2281 ctactattga aggggaaacc gatatgccgc aaaatgcgcc caagctgcag ccgcaccttc
     2341 agctcgattc cgcccacggc gccgtctcgg cgctgctgcc gggcgatccc gcgcggctcg
     2401 accggatcaa gccgtttctg accgataccg tggaactcgc ttacaaccgc gagtttcgaa
     2461 gccttgctgg aacctatcag ggcgcgcgcg tgctcgccgt ttccaccggc atcggcgggg
     2521 cttccgccgg aatcgccgtg gaggagctgc ataacctcgg catacaaagc atgatccgca
     2581 tcggaagctg cggcgcgctc cagccgcaag tgaaacttgg cgatctgatc ctcgtttccg
     2641 gcgcggtgcg ggacgacggc gcgagcaaag catatatcga cccgatctac cccgccgtcg
     2701 cggacgctgc tctgctcgcc gcctgcgaaa aagtcgcgca aaagctcggc tttgcgttcc
     2761 acacgggaat tgcgcgcagt cacgacagct tctataccga ccgcgaggag gaaatcgacg
     2821 cgtactggtc gcaaaaaggc gtactggggt gcgacatgga gaccgccgcg ctgtacgtca
     2881 tcgggcggct ccgacagatt cgctgcatga gcatcctcaa taacgttgtc gcgtaccaag
     2941 ccgatacggc ggacgccatc ggctcgtacg ccggcggcga ggacgcggcc atgcggggcg
     3001 aggaacacga aattgtcacc gcgctggaag cgctggcaag cctttcggca taagcaaaaa
     3061 atcgaatcca gaacaacggt tccgaaccat acggagccgt tttttattcc gcgccatatt
     3121 gcgccatggt ttcgaacagg cggcgcttga gcgcttcccg gtctacaccg gtggcaaccc
     3181 atgcgttctt ctctgtcttt ttatcgctgt acaagtcggt cacggtcttg ccgcgcgtga
     3241 gcctgccttt ggtctccacg cggacgcccg cgcgctcggt ttttagcagc gcttcatcgt
     3301 cggcatacag cagcgcaagg gggtcgtgca tgcacatgcc cggcaggccg tagccatgcg
     3361 aata
//
LOCUS       DAOMGY010000093         3354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30092_length_3354_cov_3.026675, whole genome shotgun sequence.
ACCESSION   DAOMGY010000093 DAOMGY010000000
VERSION     DAOMGY010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30092_length_3354_cov_3.026675"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2201)
                     /locus_tag="PLR57_02215"
     CDS             complement(<1..2201)
                     /locus_tag="PLR57_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HQQ40298.1"
                     /translation="MGELKYVGKTVDRCDAVKKVTGAAEYVDDIHLPRMLYAQCVRSP
                     YAHAKIISVDLTEAKKLPGVRCAICGDEYPNRAGLYLEDRYFMAKPGDTAKYMGEAVA
                     AVAAETPEIAQQACDLIKVVYEELPAVTNALDGMKPDAPVIHPDLGKYKVAPIFYPVP
                     GTNISHHYVLRKGDFEAAAAEADAVFENSFYIPHVQHVPLEPHACMAKYEADGKLTVW
                     ASCQSPYAVRQALSVAFDMALNKIRVISPTVGGGFGSKAGTTLEGIVIPLALHCQGRP
                     VKLVYSREDEFVNAYVRQGMHATIKTAVKKDGTILGVKNDFVWDGGAYTEYGVNITKA
                     GGWASAGPYDIDNVWTDSYCVYTNHPVGGPYRGFGMCEIHFAIEQNIDMVAEAIGISD
                     VEIRRINGIRPGGFTGMGEKMDVAGYQDCLETVLKDMEYEKPSVQPENPKKIRAKGLA
                     SGWKSPSMPTNAGSSAIIRMNEDGTFFLMSSGQEIGQGAATVLSQIAAEVLSVPPSKI
                     TYVSGDTDHTPYEWQTVASRTTYCAGNAIKLAAEDLKDKVLDLAQVKMGAMKRDLDFE
                     DGYVVHKIYPERRLPISEFALGVKFDDGSGYGGPAIGVGSFTLENNINTDKKTGVAKH
                     HPAAFWTMGVNGAEVEVDTETGVIKVLKMCSCFDAGKVINPALFCAQSEGAMVQALGT
                     AIFEELKLKNGRVLNKSFMDYKIPTADDIPELVVKYVEHAEPTGPYGARGVG"
     gene            complement(2201..2698)
                     /locus_tag="PLR57_02220"
     CDS             complement(2201..2698)
                     /locus_tag="PLR57_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQQ40299.1"
                     /translation="MKKIHVSFFINEEVFELDVQPNRTMLSVIRDELHLTGTKVGCAA
                     GECGACTVIVDGKPINACLVLAPEMDGMHITTIEGIADHDGNLSPLQKSFVEHAALQC
                     GFCTPGFIMSATALLNENPTPTRDEIKHAISGNLCRCTGYIRIIEAIEDVAKQNADQA
                     KGGAN"
     gene            complement(2712..>3354)
                     /locus_tag="PLR57_02225"
     CDS             complement(2712..>3354)
                     /locus_tag="PLR57_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQQ40300.1"
                     /translation="KQNGLVIGACATVAAISADENVKKYYPFLAEAAESIGSKQVRNR
                     ATCIGNIVNASPLCDTGTPLYALNAVVLLESASGKREVPITEFITFVRKTVLKEDELV
                     TAIRVPYVEGVKGVFTKVARRKEVDLSTICGTVVKDGNGFRIALGAVAPTPVRLPKTE
                     ALLSGKPLTDALIDEAAKLAQTEVSPIDDVRASKAYRLDVTEVIVKNGLRAVR"
BASE COUNT          599 a         1017 c          985 g          753 t
ORIGIN      
        1 cctacgccgc gcgccccgta tgggccggtg ggttccgcgt gctcaacata ttttaccaca
       61 agttcgggta tgtcgtcagc agtgggaatc ttgtagtcca taaagctctt gttgagcacg
      121 cgcccgttct tgagcttgag ttcttcaaag atcgccgtgc cgagcgcctg caccattgcg
      181 ccttcgctct gcgcgcagaa gagagcgggg ttgatgacct tgcccgcgtc gaagcaggag
      241 cacatcttga gcaccttgat cacgccggtc tcggtatcca cctcgacctc cgcgccgttg
      301 acgcccatgg tccagaacgc cgccggatgg tgcttggcaa cgcccgtctt cttgtcggta
      361 ttgatgttgt tttccagcgt aaacgagccg acgccgatag cgggtccgcc atagccggag
      421 ccgtcgtcaa acttcacgcc cagagcaaac tccgaaatcg gcagcctgcg ttcggggtaa
      481 atcttgtgca cgacgtagcc gtcctcaaag tcgaggtcgc gcttcatggc gcccatcttc
      541 acctgtgcaa ggtcaagcac cttgtccttg aggtcttccg cggcaagctt gatagcgttg
      601 cccgcgcagt aggtcgtgcg gctcgccacc gtctgccatt cgtagggcgt atggtcggtg
      661 tcgccggaaa catacgtgat cttggaaggc ggaacggaga gcacctccgc cgcgatctgc
      721 gaaagcaccg tcgccgcgcc ctggccgatt tcctgcccgc tggacatgag gaagaacgtt
      781 ccatcctcgt tcatgcggat gatcgcgctg ctacccgcgt tggtcggcat ggaaggcgac
      841 ttccagccgc tggcaaggcc ctttgcgcgg atcttcttcg gattttcggg ctgcacgctc
      901 ggcttttcgt actccatatc cttgagcacg gtttcgaggc agtcctgata gcccgcaacg
      961 tccatctttt cgcccatgcc ggtgaacccg cctggacgaa tgccgttgat gcggcggatt
     1021 tccacgtccg aaatgccaat cgcttcggcg accatgtcga tgttctgctc gatggcgaaa
     1081 tggatttcgc acatgccgaa cccgcggtac ggcccgccga cgggatggtt ggtgtagacg
     1141 cagtagctgt ccgtccagac gttgtcgatg tcgtacggac cagcgctcgc ccatccgccc
     1201 gccttggtga tgtttacgcc atactcggtg taggctccgc cgtcccagac gaaatcgttc
     1261 ttaacgccaa ggatcgtgcc gtccttctta accgcggtct tgatggtcgc gtgcatgccc
     1321 tggcggacat acgcgttgac gaactcgtcc tcacgggagt agaccagctt gaccggcctg
     1381 ccctgacaat gcagcgcgag cggaatgaca attccttcca gcgtcgtgcc ggccttgctg
     1441 ccgaatccgc cgccgaccgt aggcgaaatg acgcggattt tattgagcgc catatcgaac
     1501 gcaacggaga gcgcctgccg cacggcgtac ggagactggc agcttgccca aacggtcagc
     1561 ttgccgtccg cttcgtactt ggccatgcag gcgtgcggct ctagcggcac gtgctggaca
     1621 tgcggaatat agaacgagtt ttcgaaaaca gcgtccgctt ccgcggcggc ggcttcaaaa
     1681 tcgcccttgc gcagtacgta atgatgcgag atattcgtgc ccggcacggg gtaaaagatg
     1741 ggagctacct tgtacttgcc aaggtcgggg tggatgaccg gcgcgtccgg cttcatgccg
     1801 tccagcgcgt tcgtcacggc gggcagttcc tcatacacga ccttgatcag gtcgcaggcc
     1861 tgctgcgcta tttccggcgt ttccgccgca accgccgcta ccgcctcgcc catatacttg
     1921 gcggtgtcgc ccggctttgc cataaagtac cggtcttcca gatacaggcc cgcgcggttg
     1981 gggtattcgt ccccgcagat ggcacagcgc acgcccggca actttttcgc ttccgtcagg
     2041 tcgaccgaaa tgatctttgc gtgcgcgtac gggctgcgga cgcactgggc atagagcatg
     2101 cgcggcagat ggatatcgtc cacgtattcg gcggcgcccg ttactttttt caccgcgtcg
     2161 cagcggtcaa ccgtcttgcc gacatatttg agttcgccca tcagttcgca ccccctttcg
     2221 cttgatctgc gttctgtttt gcaacgtctt caatggcttc gataatgcgg atataccccg
     2281 tgcagcggca gaggttgccg gagatggcgt gcttaatctc gtcacgcgtc ggggtcgggt
     2341 tttcgttcag aagcgcggtt gcgctcataa taaagcccgg cgtgcagaag ccgcactgaa
     2401 gagcggcgtg ctccacaaac gatttctgca gcggagaaag gttgccgtcg tgatcggcaa
     2461 tgccctcgat ggtggtaata tgcatgccgt ccatttcggg cgcgagcacg aggcaggcgt
     2521 tgatgggttt accgtccaca atcaccgtgc aggcgccgca ttcccccgcc gcgcagccga
     2581 cctttgtgcc ggtgaggtgc agttcgtcgc gaatgacgct gagcatggtg cggtttggct
     2641 ggacgtcaag ctcaaaaact tcttcgttga tgaagaaact gacgtgaatc tttttcatgc
     2701 gctttccctc cttaccgaac cgcccggagg ccgtttttca cgatgacttc cgtcacgtcc
     2761 agccggtatg ccttgctggc gcgaacgtcg tcgatgggag aaacctccgt ttgggcgagc
     2821 tttgccgctt cgtcaatcag cgcgtccgtg agcggtttgc cgctaagcag cgcttccgtt
     2881 ttgggcagac gaacgggcgt cggcgcaacc gcgccgagcg cgatgcggaa gccgtttcca
     2941 tcctttacta ccgtgccgca gatggtcgaa agatcgactt cctttctgcg ggcgaccttg
     3001 gtaaacacgc ccttcacgcc ttccacgtaa ggaacgcgga tggcggtaac gagttcgtct
     3061 tccttgagca cggttttccg cacgaaggta atgaattccg taatcggcac ttctcgtttg
     3121 ccagaagcgc tttcgagtaa gactacggca ttcaacgcgt agagcggcgt gccggtgtcg
     3181 cagagcggag aagcgttgac gatgttgccg atgcaggtgg cgcggttacg cacctgcttg
     3241 ctgccaatgg actccgccgc ttccgcaagg aacgggtaat actttttcac gttctcgtcc
     3301 gcggaaatcg cggcaaccgt cgcgcacgcg ccgattacaa gaccgttttg ttta
//
LOCUS       DAOMGY010000094         3348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30171_length_3348_cov_4.179168, whole genome shotgun sequence.
ACCESSION   DAOMGY010000094 DAOMGY010000000
VERSION     DAOMGY010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30171_length_3348_cov_4.179168"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLR57_02230"
     CDS             <1..406
                     /locus_tag="PLR57_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HQQ40301.1"
                     /translation="EALFFRHELKKTYHAIVRGTPPKSGRLVNWVKKDAEESFVSICG
                     ENDPDALRCELTYRVIRSDGEKSLLEIELMTGRTHQIRVQMAAAGYPVLGDDKYGDRA
                     FNKAQKRRTQALLAKRLELDGHVFESLRELEL"
     gene            complement(510..2009)
                     /locus_tag="PLR57_02235"
     CDS             complement(510..2009)
                     /locus_tag="PLR57_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQQ40302.1"
                     /translation="MCYHTPMERPAKTKKLYQSASYVTSNAVFYGTLLFFVSRVFGFL
                     QLFIDAYNRKKLGQAPPENFTVLGIIGSILVLGVITGAFLLLVQYRQKKLRLRKGNFF
                     LLLNVLFVVWGVIQAINYLVEMILFFEFVYLLDFLTLLGALALPSVLLQLSDRKQEPV
                     QEQILLYAGIGSTALSVIATLIVALVLRKSYTAFQLVRELMFRGGIILFGVAVLQKAI
                     KLRSEPLPAYTPKPEAKKPAPKKTVLRDPVYTPDKSGKIECPDCGKKVAVGTAICPRC
                     GYDMNTPSLFEEDEEEIITPPEEPEEEEVFVPEPEEPAPEPVKSDLCPRCGKKIPEPL
                     VTCPRCGYYPGDSLEMPKEPEKRAPVPEPRQRSAEPKRERTCAKCGKKLPGNLATCPY
                     CGYHPSDEELAAEQAEEYDDYGEPEDGVPCPRCDRLVAHGTQICPHCGYPFPDETARH
                     PRPAPPKLPRVPDPKYLTEKNSIDCPECGRRYPASRSVCPYCGYSLYED"
     gene            2161..>3348
                     /locus_tag="PLR57_02240"
     CDS             2161..>3348
                     /locus_tag="PLR57_02240"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HQQ40303.1"
                     /translation="MQSNAQAIFESWKNEPSLDAATRAELTAIENDPREIEERFYREL
                     EFGTAGLRGIIGAGTNRMNAYVVRRATQGLCDYLATVPGAKERGVCVAYDSRLFSDEF
                     AKQTACVLAGNGVKTYLFTSLHSVPQLSYAVRHLGCMAGIVITASHNPKQYNGYKVYW
                     AYGGQAAPEQAAAIYEMIQNVKMFAASECDFEGAVHDERIVLVGKEEDEAYYAATAGL
                     LLNPDLLRKFGGTLPIVYTPLHGSGAIPVKELLERVGLTSVTVVPEQAAPDGTFPTVT
                     APNPEDPAAFRLAIALAEKVRARVCLATDPDADRLGVAVKTANNEWVTLTGNQIGCIL
                     LEHILSSLRSQGRLPENGVVIKSIVSTRLADAICKAYGVELEDVMTGFRFIGEKVDEY
                     QSSG"
BASE COUNT          696 a          962 c         1051 g          639 t
ORIGIN      
        1 cgaagcgctg tttttccggc acgagcttaa aaagacatat cacgccatcg tgcgcggaac
       61 gccgcccaag agcggaaggc tcgtaaactg ggttaaaaag gacgcggagg aatcgtttgt
      121 ttccatttgc ggtgaaaacg atccggatgc tctgcgctgc gaactgacgt accgggtgat
      181 cagatccgac ggcgaaaaga gcctgctgga gattgagctg atgaccgggc gcacgcacca
      241 gattcgcgtg cagatggcgg cggcgggcta tcccgtgctg ggggacgata aatacggcga
      301 ccgcgccttc aacaaggcgc agaaacgcag aacgcaggcg ctgctcgcca aacgtctgga
      361 gctcgacgga catgtgttcg agagcttgcg agagttggaa ctataagaaa ttaaaacgct
      421 tctcaaccga gaagcgtttt gtatttcacg cggggttcgc aggggtgtgc cgctttgcgg
      481 gagttcgagg gcggagccct cggcgtcttc tagtcctcat acaagctata cccgcaatag
      541 gggcagacgc tccgcgaggc gggatagcgt cttccgcatt cgggacaatc gatgctgttc
      601 ttctcggtga ggtacttggg gtcgggcacg cgcgggagct tcggcggagc gggccgcgga
      661 tggcgcgccg tctcgtccgg gaagggatac ccgcagtgcg ggcaaatctg cgtgccgtgc
      721 gcaaccagcc ggtcgcagcg cgggcagggg acgccgtcct ccggctcgcc gtaatcgtcg
      781 tattcttcgg cctgctcggc ggcgagctcc tcgtccgagg ggtgatagcc gcagtagggg
      841 caggtggcga ggttgccggg gagcttcttg ccgcatttgg cgcaggtgcg ttcccgtttc
      901 ggttcggcgc tcctctgcct tggttcgggc acgggggcgc gtttctccgg ctcctttggc
      961 atctccagag agtcgcccgg gtaatacccg cagcgcgggc aggtgacgag cggttcggga
     1021 atcttctttc cgcagcgcgg gcaaaggtcg cttttgaccg gttccggcgc gggttcttcc
     1081 ggttccggaa caaaaacctc ttcctcttcc ggctcttcgg gcggcgtgat aatttcttcc
     1141 tcgtcctctt caaagagact gggcgtgttc atgtcatacc cgcagcgcgg gcagatggcg
     1201 gtgccgacgg caactttctt gccgcagtcc gggcactcga ttttgccgga tttgtccggc
     1261 gtgtagacgg gatcgcggag aacggtcttc ttcggcgcgg gctttttcgc ctccggcttc
     1321 ggcgtatagg cgggcagcgg ttcggaacgg agcttgatgg ctttctgcag caccgctacg
     1381 ccaaagagga tgattccgcc gcggaacatc agctcgcgca caagctgaaa tgccgtatag
     1441 ctcttgcgca gaacgagcgc aacgatgagc gttgcgatta ccgagagcgc ggtggaacct
     1501 atgcccgcat agagcagaat ctgctcctgc acgggctcct gcttgcggtc ggaaagctga
     1561 agcagtaccg aaggcaacgc cagcgcgccg agcagcgtta aaaagtccag caggtataca
     1621 aactcgaaaa acaggatcat ctcgaccaga tagttgatgg cctgtatcac gccccagacg
     1681 acaaagagca cgttgagcag caggaaaaag ttgcccttgc gcaggcgcag tttcttctgg
     1741 cggtactgca ccagcaaaag aaacgccccc gtaatgacgc cgaggacgag gatggagccg
     1801 atgatgccga gcaccgtaaa gttctccggc ggcgcctgcc ccagtttttt tcggttgtac
     1861 gcatcgataa agagctgtaa aaagccgaac acccgcgaaa caaagaaaag aagcgtacca
     1921 tagaacaccg cgttgctcgt tacatagctt gcggactggt agagcttttt cgttttggca
     1981 ggccgttcca taggggtatg atagcacaag cccgccttgc catgcaactc ggggaacggt
     2041 ataatgtaat cggaagtata tcgaaaaagc gaccgaaaac cctgccgttc gggactgcgt
     2101 tctttgaacc gaccgttccg gacgtggcag atatttatat caagagagag gattaccgcc
     2161 atgcaatcaa acgcgcaagc gatttttgaa tcctggaaaa acgaaccctc gctggatgca
     2221 gcgaccagag ccgaactgac ggccatagaa aacgacccgc gcgaaattga agagcgcttt
     2281 taccgcgagc tggagttcgg cacagcgggc ttacggggca tcatcggcgc gggcaccaac
     2341 cgcatgaacg catacgtcgt ccgccgggcg acgcagggcc tttgcgacta cctcgcaacc
     2401 gtgccgggcg caaaggaacg tggcgtctgc gtggcgtacg actcgcgcct cttttcggac
     2461 gaatttgcca aacagaccgc ctgcgtgctc gcgggaaacg gcgtaaaaac ctacctgttt
     2521 acctcgctgc attccgttcc gcagctttcc tacgcggtgc ggcatctggg ctgcatggcg
     2581 ggcatcgtga ttaccgcctc gcataacccc aagcagtata acggctacaa ggtatattgg
     2641 gcgtacggcg ggcaggcggc tcccgaacag gcggccgcta tctacgaaat gattcagaac
     2701 gtaaaaatgt tcgccgccag cgagtgcgat tttgaagggg ctgtgcacga cgagcgtatt
     2761 gtactcgtcg gcaaagaaga ggacgaggcg tactacgccg cgacggcggg acttttactc
     2821 aaccccgatc tgctgcggaa attcggcggt acgctgccca tcgtgtatac gccactgcac
     2881 ggctccggcg cgattcccgt caaggaactg ctggaacgcg tcgggctgac cagcgtcacc
     2941 gtcgtgccgg agcaggccgc gccggacgga acgtttccaa ccgtgaccgc gccgaacccg
     3001 gaagaccctg cggcgttccg cctcgccatc gcgctggcgg aaaaggtgcg cgcgcgcgtc
     3061 tgcctcgcca ccgacccgga cgcggaccgg ctgggcgttg ccgtcaagac cgcgaacaac
     3121 gagtgggtca ctctaaccgg caaccagatc ggctgcatcc tgctcgagca cattctctcc
     3181 tcgctccgct cgcaggggcg gctgccggaa aacggcgtgg tgatcaagtc catcgtttcc
     3241 acacgtctgg cggacgcgat ctgcaaagct tacggcgtgg agctggaaga cgtgatgacg
     3301 ggattccgct tcatcggcga aaaggtggac gagtatcagt ccagcggg
//
LOCUS       DAOMGY010000095         3344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30219_length_3344_cov_3.423229, whole genome shotgun sequence.
ACCESSION   DAOMGY010000095 DAOMGY010000000
VERSION     DAOMGY010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30219_length_3344_cov_3.423229"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="PLR57_02245"
     CDS             <1..129
                     /locus_tag="PLR57_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40304.1"
                     /translation="ALKEEIQEYVKAHTAPYKYPRIVEFRESLPKTTSGKIMRRLL"
     gene            194..841
                     /locus_tag="PLR57_02250"
     CDS             194..841
                     /locus_tag="PLR57_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40305.1"
                     /translation="MNAKRVTVLTGHYGSGKTSLAVNWALKLSESGRAVTVADLDIVN
                     PYFRTTDRRRELEARGIRLIASQFANSNLDLPALPAELYSVVESREGYAVLDVGGDDR
                     GAVALGRYAPFLLAEGDYEMLFVVNFCRPLTRTALEALGVLREVEAACGLAATALVNN
                     SNLGRETTADTVLQTVERMRELSQLANLPVKMTAVSRELKDQLEGNIENLFPMDL"
     gene            909..1115
                     /locus_tag="PLR57_02255"
     CDS             909..1115
                     /locus_tag="PLR57_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQQ40306.1"
                     /translation="MAKLTFETDLCKGCGLCIDACPKHILKIDRETINQKGHSPATLT
                     DESACTGCGSCATMCPDCVITVER"
     gene            1128..2192
                     /locus_tag="PLR57_02260"
     CDS             1128..2192
                     /locus_tag="PLR57_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HQQ40307.1"
                     /translation="MSEKVLMKGNEAIAEAAIQAGCRHYFGYPITPQTEIAAYMAKRM
                     PKINGVFLQAESEIAAINMVYGVSASGRRVMTSSSSPGISLKSEGISYLAGSDLPALI
                     VNVQRGGPGLGGIQPSQADYFQATRGGGHGDYHLIVLAPASVQEMASLTALGFDLADQ
                     YSMPAMILADGTIGQMMEPVALETSEAKKVEKPWALTGTNKTRAHNIVTSLYLQPEEL
                     ERTNIARFQRYAEIEAKEPRFESYLMDDAEICVVAYGIAARVSRNAVDAAREKGIRAG
                     LIRPITLWPFPKQAMQAAAKQVKAFLSVELSMGQMIEDIELSIRCSKPVTLINRVGGM
                     IPSPEQVLAGIEQAAGGEQK"
     gene            2189..2935
                     /locus_tag="PLR57_02265"
     CDS             2189..2935
                     /locus_tag="PLR57_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQQ40308.1"
                     /translation="MIVAKRPDSLLPAATHYCPGCTHGIIHRLVAEAIDTLGIEGKTI
                     GVAPVGCAVLAYKYFACDMIEAAHGRAPAVATGLKRADPNTIIFSYQGDGDLASIGMA
                     ETVHAAARNENITIIFINNAIYGMTGGQMAPTSLPGQVTQTSPYGRDVTQCGYPIRVC
                     EMLSELEGPEYLERVTVNSVKNVRAAKKAIEKAFRNQIEGKGFSLIEVLSACPTNWGM
                     TPQDALKWIDDKMIPYYPLGVFKDRSGKGA"
     gene            2939..>3344
                     /locus_tag="PLR57_02270"
     CDS             2939..>3344
                     /locus_tag="PLR57_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQQ40309.1"
                     /translation="MEQQKNTKAKSMQVLIAGFGGQGVLFAGKFLAYLGMIGERQVSW
                     LPSYGPEMRGGTANCSVILSDAPIGSPIVSAPDCLIAMNLPSFQKYQSQVKSGGVIVL
                     DSSLIFNQPERGDLTVCSVPATQLAKDSGLEGL"
BASE COUNT          762 a          977 c         1003 g          602 t
ORIGIN      
        1 gcgctcaagg aagagataca ggagtatgtc aaagcgcata ccgctccgta taaatacccg
       61 cgcatcgtgg agttccgcga atctctcccc aagaccacga gcggaaagat catgcgcaga
      121 ctgttataat gaaagaatct cagcccgctt ttagcggaat gacgatggac gggacatgaa
      181 ggagaacggg aatttgaacg caaagcgcgt cacggtgctc accggccact atggcagcgg
      241 taaaacgagc ctcgccgtca attgggcgct caagctcagc gaaagcggac gcgcggtcac
      301 ggttgccgac ctcgacatcg taaacccgta tttccgcacc accgaccgca ggcgagaact
      361 ggaagcgcgc ggcatacggc tgatcgcatc ccagtttgcc aacagcaacc tcgatctgcc
      421 cgctctgccc gccgaactct actcggttgt ggaaagcagg gaagggtacg cggtgctgga
      481 cgttggcggg gacgacaggg gcgcggtcgc gctgggacgg tacgcgccgt ttctgctggc
      541 cgaaggcgat tacgaaatgc tctttgtggt caacttctgc cggccgctga cccgaaccgc
      601 tctcgaagct ttgggcgtgc tgcgcgaggt ggaagccgcc tgcggactgg ccgcaaccgc
      661 gctggtcaac aacagcaacc ttggcaggga aacgaccgcg gataccgttt tacagacggt
      721 ggaacgcatg cgggagcttt cgcagcttgc aaacctgccc gtcaagatga cggcggtcag
      781 ccgggagctg aaagaccaat tggagggaaa catcgaaaac ctgtttccaa tggatttata
      841 aaaagaagcg gcgatttaac tttcgagata atcgccgcgg aacgtacagg aaagggacaa
      901 aggcgaacat ggcaaaactg acgtttgaaa cggatctctg caagggctgc ggtctctgca
      961 tagacgcatg cccgaagcac atcctcaaaa tcgatcgcga aacgatcaac caaaaggggc
     1021 attcgcccgc aacactgacg gacgaatccg cctgcacggg ctgcggttcg tgcgcaacga
     1081 tgtgccccga ttgcgtgatc acggtggaaa ggtaaggaga acaagaagtg tccgaaaaag
     1141 tactgatgaa ggggaatgaa gccatcgccg aagcggccat tcaggcggga tgccgccact
     1201 acttcggtta cccgattacg ccgcagacgg agattgcggc ctacatggca aagcgcatgc
     1261 ccaagatcaa cggcgtgttt ctgcaggcgg agagcgaaat tgccgccatc aacatggttt
     1321 acggcgtgtc cgcctccggc aggcgcgtta tgacgtcttc ttcgagcccc ggaatctcgc
     1381 tcaagagcga ggggatttcg tatctcgcgg gcagcgacct tcccgcgctg attgtcaacg
     1441 tacagcgcgg cggacccggc ctcggcggga ttcagccttc gcaggcggat tactttcagg
     1501 ctacgcgcgg cggcggccac ggggactatc atctcatcgt gctcgcgccc gcttccgtac
     1561 aggagatggc gagtttgacc gcgctggggt tcgatcttgc ggatcagtat tccatgcccg
     1621 ccatgatcct tgccgacggg acgatcgggc agatgatgga gcccgtagcg ctggaaacga
     1681 gcgaagcaaa gaaggttgaa aaaccctggg cgctcaccgg cacgaacaaa acgagggcgc
     1741 ataatatcgt gacctcgctc tacctccagc cggaagagct ggaacgcacg aatatcgccc
     1801 ggtttcagcg gtatgccgaa atcgaagcaa aggaaccgcg ctttgaatcg tacttgatgg
     1861 acgacgcgga aatctgcgtg gtcgcctacg gcatcgccgc gcgcgtttcc cgcaacgccg
     1921 tggacgccgc ccgcgaaaag ggtatccgcg cgggtctcat ccgcccgatt acgctctggc
     1981 cgttccccaa acaggcaatg caagccgccg caaagcaggt caaggctttc ctttccgtgg
     2041 aactctcgat gggacagatg attgaggata tcgagctttc gatccgctgc tccaagcccg
     2101 tgacgctgat caaccgcgtt ggcggcatga tcccctcgcc ggagcaggtg ctcgccggca
     2161 tcgaacaggc cgccggaggt gaacagaaat gattgtagcg aaacgtcccg attctctttt
     2221 gcccgcagca acgcattact gcccgggctg cacgcacggc attatccacc gcctcgtggc
     2281 ggaagccatc gataccctcg gcatcgaagg aaaaacgatc ggtgttgctc ctgttggctg
     2341 cgcggtgctg gcttacaagt actttgcctg cgatatgatc gaagctgcgc acgggcgcgc
     2401 gcccgccgta gcgacggggc tcaagcgcgc cgatccgaac acgattatct tttcgtatca
     2461 gggcgacggc gaccttgcga gcatcggcat ggcggaaacc gtacacgccg cggcgcgcaa
     2521 cgaaaacatc accatcatct ttatcaacaa cgccatctac ggcatgaccg gcggacagat
     2581 ggcgccgact tcgctccccg gacaggtcac gcagacgtcc ccttacgggc gggacgttac
     2641 gcagtgcggc tacccgattc gcgtctgcga aatgctctcc gaactcgaag gaccggaata
     2701 tctggagcgc gtgaccgtca acagcgtcaa aaacgtccgc gccgcgaaga aagccattga
     2761 aaaggcgttt cgcaaccaga tcgaaggcaa ggggttttcg ctcatcgaag tgctttccgc
     2821 ctgcccgacc aactggggca tgaccccgca ggacgcgctt aagtggattg acgacaagat
     2881 gattccgtac tatccgctcg gcgtgtttaa agaccgcagc ggaaaggggg cgtgaaccat
     2941 ggagcagcag aaaaacacaa aagcgaaaag catgcaggtt ttaatcgcgg gcttcggcgg
     3001 acaaggcgtc ctgtttgcgg gcaagtttct tgcttacctc ggcatgatcg gcgagcggca
     3061 ggtgagctgg cttccctcgt acgggccgga gatgcgcggc ggcacagcca actgcagcgt
     3121 cattctcagc gacgctccca tcggctcgcc catcgtctcc gcgccggact gtttgatcgc
     3181 aatgaacctg ccgtcgtttc agaagtacca aagtcaggtc aaaagcggcg gcgtgatcgt
     3241 acttgattct tcgctgattt ttaatcagcc ggagcgcggc gatttgaccg tctgcagcgt
     3301 acccgcgacc cagcttgcca aggacagcgg gctggaaggc ctcg
//
LOCUS       DAOMGY010000096         3333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30371_length_3333_cov_2.715985, whole genome shotgun sequence.
ACCESSION   DAOMGY010000096 DAOMGY010000000
VERSION     DAOMGY010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30371_length_3333_cov_2.715985"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PLR57_02275"
     CDS             <1..1021
                     /locus_tag="PLR57_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ40310.1"
                     /translation="RDRFSYLWTAVGICQMLRSGVTAFFNMDIKYAQDGMFRAAKEAN
                     VRGYIGLELADLFLSDEKGLRRDLSEVEWLLKTYPETCVVTPSEPNLCSEAALSALSA
                     LAKQYGALIQVHVDETALEAAQCVSERGKTELEYLDSLGFLSPRFSAVHGVHLTEREI
                     ELAAQRGVTVVYNPKSNAKLGSGVCPIPALNQAGINISLATDGPASNDRLDMFEEMRF
                     GAMLQKAALSNPEAVTASDVFAMATAGGANMLLLDAGELKEGKLADFSILPLDNPRLA
                     FGTADLISTIVYCAESGDVRDVYVGGKPALKNGVVTGFDESAVTSEFYGILHRLLDEI
                     QALPK"
     gene            1154..2122
                     /locus_tag="PLR57_02280"
     CDS             1154..2122
                     /locus_tag="PLR57_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HQQ40311.1"
                     /translation="MQTLSERTVNIIEERILPRLWELNCERHTLKNGATVIDMGIHAP
                     GGREAALLFTLVTLGGLGEVGYGLIPVGGKMLESIEVRLDRPQEAALSSQFSAWKIKP
                     TGEYQLPGFASGPARAIAYRDPIAKMWPYRETFHKTALAIQTAELPDESIAEEVCSAC
                     GVEPQNVYLLAAKTGSLVGCVQIAARMAEVSLWGLGYNGFPLDQVLSFSGSAAIPPVI
                     SDELIAMDRVNSSTLYGSVARYIVDCEDAEIERVLPHMAFSGTKHNKKTFAELYDAAE
                     RDIFKMDTDVHKVARIEMTNLRTGRTFYAGALDAEMLVQSYYENVK"
     gene            2175..>3333
                     /locus_tag="PLR57_02285"
     CDS             2175..>3333
                     /locus_tag="PLR57_02285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ40312.1"
                     /translation="MQSGNQPFDLQYDVAVVGGGVVGAAAAWKLAKAGAKVALLEKGD
                     LCSGASSTNPGFCVLSYRENSLTMRFALEQQRSWDALQAEIGDVEYVPSGGLIPLTDH
                     AQEDILSGLCRHAKKLGLDEIGLITAAKAKELEPALDESQIVGGCWCPGEGRVNPFKM
                     NLNMASKAKSLGADIFTHTPVTNLRIENGLVRALETPRGEIKADLFILANGAWARELA
                     SLAGHDIPILFERGEAMVSMQVAPRVKRMITDGALFNQSPADHPMVIGACLGQTVSGN
                     IVIAQATTRPDDFDKSNSFEGPRLIAKRVLKLFPSLKDIEIIRMWAGLVSYTDDHQPV
                     FGAFANPNNLFVANTFHSAVAISPAIGGMIADYWKTGSLPEEAKPYTPERFA"
BASE COUNT          711 a         1046 c          940 g          636 t
ORIGIN      
        1 acgcgatcgc ttctcctacc tctggacggc ggtcggaatc tgccagatgc tgcgctccgg
       61 cgtgaccgcg tttttcaaca tggacatcaa gtacgcgcag gacggcatgt tccgcgcggc
      121 aaaggaagcg aacgtccgcg gctatatcgg gctggagctt gccgacctgt ttttaagcga
      181 cgaaaaagga ctccggcgcg acctttcgga agtcgagtgg ctgcttaaaa cttacccgga
      241 aacctgcgtc gtcaccccgt ccgaaccgaa cctctgcagc gaagccgctc tttccgcgct
      301 ctctgcgctt gcaaagcagt acggcgcgct cattcaggtg catgtggacg aaaccgcgct
      361 ggaagccgcg cagtgcgttt ccgaacgcgg caaaaccgag ctggaatacc tagattctct
      421 cgggttcctt tcgccccgct tctccgccgt gcacggcgta cacctcaccg agcgggaaat
      481 cgagctcgcg gctcagcgcg gcgttacggt agtctacaat cccaaaagca acgccaaact
      541 cggcagcggc gtctgcccca tcccggcgct taatcaagcg ggtatcaaca tctcccttgc
      601 cacggacggc cccgccagca acgaccgtct cgatatgttc gaggagatgc gctttggcgc
      661 gatgctccag aaagccgcgc tttcgaatcc cgaagccgtc accgcgtcgg acgtgtttgc
      721 aatggcgacc gcgggcggcg cgaatatgct cctgctcgac gcgggcgaac tcaaggaagg
      781 caagctcgcg gacttctcca ttctcccgct cgacaatccc cgccttgcgt tcggaacagc
      841 cgatctcatc tccaccatcg tctactgcgc cgaaagcggg gatgtgcgag atgtttacgt
      901 cggcggcaaa cccgcgctga aaaacggcgt ggtcacgggt ttcgacgaat ccgccgtcac
      961 aagtgaattt tacggcatcc tgcatcgtct tctggacgaa atacaagcgc ttcccaaata
     1021 aagagatttt acggcattct gcgtcgtctt ctggacgaaa tacaagcgct ttccaagtaa
     1081 tctaacaagc atcaatctaa caagcatcaa tctaacaagc atcaatctaa caagcatcaa
     1141 atggagggat tccatgcaga ctctgagtga acgaaccgtc aacataatcg aggaacggat
     1201 tcttccgcgc ctctgggaac tgaattgcga acggcacacg ctgaaaaacg gcgcaaccgt
     1261 gatcgatatg ggtatccacg cccccggcgg gcgggaagcc gccctgctct ttacgctcgt
     1321 gacgctcggc ggcctcgggg aagtcggcta cgggctgatc cctgtcggcg gcaaaatgct
     1381 ggaaagcatc gaagtgcgtc tcgaccgtcc gcaggaagcg gcgctctcct ctcaattttc
     1441 cgcctggaag atcaagccca ctggcgaata ccagttaccc ggctttgctt ccggtcccgc
     1501 gcgcgcaatc gcttaccgcg acccgatcgc aaagatgtgg ccgtatcgcg aaaccttcca
     1561 caaaaccgcg ctcgccatcc agaccgcgga actgccggac gaatccatag ccgaggaagt
     1621 ctgctccgcc tgcggcgtgg aaccacagaa cgtgtacctg ctcgcggcta aaaccggcag
     1681 cctcgtcggg tgcgtgcaga ttgccgcgcg catggcggaa gtctccctct ggggactggg
     1741 ctacaacggt ttcccgctcg atcaggtgct ttcgttctcc ggcagcgcgg cgattccgcc
     1801 cgtaatttcg gacgagctca tcgccatgga ccgcgtcaac agttccacgc tctacggttc
     1861 cgtcgcgcgg tatatcgtgg actgcgagga cgccgaaatc gagcgtgtgc tgccgcatat
     1921 ggcgtttagc ggcacgaagc acaataaaaa gaccttcgcc gaactctacg acgcggcgga
     1981 gcgcgatatc ttcaaaatgg atacggacgt gcacaaggtc gcccgcatcg agatgaccaa
     2041 tctcagaacg ggcagaacgt tctatgccgg cgcgctcgac gcggaaatgc tggtgcagag
     2101 ctattacgaa aacgtcaagt aaacgcgccg ccgcgcttca acgcgggcgc tgattcgatc
     2161 aatcgggggt attcatgcaa tcgggtaatc aaccattcga tttacagtac gacgttgccg
     2221 tcgtcggcgg cggagttgtc ggcgcggccg cggcctggaa gctggcaaag gcaggcgcaa
     2281 aagtcgccct tctggaaaag ggcgatctct gttccggcgc ttccagcacc aatccgggat
     2341 tctgcgtgct gagctaccgt gaaaacagcc tcaccatgcg ctttgcgctg gagcagcagc
     2401 gttcttggga cgctcttcag gcggaaatcg gggatgtgga atacgttcct tccggcgggc
     2461 tgattccgct taccgaccac gctcaagagg atattctctc cggcctctgc cgccacgcaa
     2521 agaagcttgg tctcgacgaa atcggcctga tcaccgccgc aaaagccaag gagctggaac
     2581 cagcgttgga cgaaagccaa atcgtcggcg gctgctggtg ccccggcgaa gggcgcgtca
     2641 acccgttcaa aatgaacctc aacatggcct ccaaagccaa atctctcggc gcggatatct
     2701 ttacgcatac gccggtgaca aacctgcgca tcgaaaacgg gcttgtccgc gcgctggaaa
     2761 cgccgcgcgg cgagatcaag gcggatttat tcatcctcgc caacggcgcc tgggcgcggg
     2821 aactcgccag tcttgccgga cacgatattc cgattctgtt tgagcgcggc gaggccatgg
     2881 tcagcatgca ggtcgccccg cgcgtcaagc gcatgatcac cgatggcgcg ctcttcaacc
     2941 agtcccccgc cgaccacccc atggtgattg gcgcgtgcct tgggcagacc gtttccggca
     3001 acatcgtcat cgcgcaggcg accacgcgcc cggacgattt cgacaagtcg aactccttcg
     3061 aaggtccgcg cctgatcgcc aagcgagtgt tgaagctctt cccctcgctc aaggatatcg
     3121 agatcatccg catgtgggct gggctggttt cgtatacgga cgatcatcag ccggtgttcg
     3181 gcgcgttcgc caatccgaac aacctgtttg tggccaacac gttccacagc gcggtcgcga
     3241 tttctcccgc catcggcgga atgattgcgg actactggaa aaccggctcg ctccccgaag
     3301 aagcaaagcc ctatacgccg gaacgcttcg cgt
//
LOCUS       DAOMGY010000097         3330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30397_length_3330_cov_3.124885, whole genome shotgun sequence.
ACCESSION   DAOMGY010000097 DAOMGY010000000
VERSION     DAOMGY010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30397_length_3330_cov_3.124885"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLR57_02290"
     CDS             complement(<1..987)
                     /locus_tag="PLR57_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQQ40313.1"
                     /translation="MKQQRVREPIDSVESMERAFTRLREAQKEFSTFSQEQVDAIFLA
                     AATAANQARIPLAKLAVEETGMGVVEDKVIKNHYASEYIYNAYRSTKTCGVIEEDKAY
                     GIVRIAEPVGVITAVIPTTNPTSTAIFKTLLALKTRNGILISPHPRAKNATIAAAKIV
                     LDAAVAAGAPEGIIDWVDSPSLELTNLAMKEADLILATGGPGMVKAAYGSGKPAVGVG
                     SGNTPAIIDDTADLLLAVSSIIHSKTFDNGMICASEQSVIVLDSVYAKVKKEFAARGC
                     YFLNAEETEKVRKTILINGALNAKIVGQTAFAIASLAGVAVPQATKILIGEVE"
     gene            complement(1372..2304)
                     /locus_tag="PLR57_02295"
     CDS             complement(1372..2304)
                     /locus_tag="PLR57_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQQ40314.1"
                     /translation="MNNLLQLKYAVEVEKTGSISKAAENLYMNQPNLSKAIRELEDDI
                     GIAIFDRTAKGVVPTEKGREFLSYARAILAQVAEMEALYHPDNDRKLRFDLCAPRAGY
                     ISRAFSALVNEIGPEADIGFSYRESGPMRVIKSVSNEVNRLGIVRCQTYYKKYFLGEL
                     TERGLKHETIRSYTRTALMSRTHPLAQKDGIDENDLTPHTEILYGDWSVPSLPVAEAR
                     QFSRIHEARKTISVFERASQMELLGTVFGAYALVSPEPESSWTCFGLVQKHCELPGNS
                     FSDLLVWRENYRMTREDLLFLEKLRASAASVPNK"
     gene            complement(2337..>3330)
                     /locus_tag="PLR57_02300"
     CDS             complement(2337..>3330)
                     /locus_tag="PLR57_02300"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ40315.1"
                     /translation="RKVAELCARYNRPLTVHPRACSAVSMAYPELLGRPHLLRALDEL
                     EEVTRGLNVKLQYSHAIFVGTSSFKCKAELVEIIERMRKNGVDAMFDLYSEICGVSVI
                     TVVMPTWYQALSKEDKRKFVNKFRFRVLANASIALLGFGFNDIQIAYIGPGFEHYEGK
                     TVHEIAKERGMNDLDAYCMLCEESDFAGRVNMGPYSTPEIITSLSRHPNSLYMTDAWV
                     EEHGVQNPAIYDCFPKFLHLSLNGKGDTLPNAIRKMTGAVAERFSLEHRGLIKPGYYA
                     DLTVFDELALKNGVPDQPKAFGIERVFVNGVCVLNGETLDEAAFRTAGRAMRVK"
BASE COUNT          626 a          908 c         1011 g          785 t
ORIGIN      
        1 ctcgacttcg ccgatgagaa tcttggttgc ctgcggcaca gccacgcccg caagggacgc
       61 aatggcaaac gcggtctggc cgacgatttt tgcgttgagc gcgccgttga tcagaatggt
      121 tttgcgtacc ttttcggtct cttccgcgtt caggaaatag cagccgcgcg cggcgaattc
      181 ctttttcact ttggcgtaaa cggagtcgag cacgatgacg ctttgttcgg aggcgcagat
      241 cataccgttg tcgaaggtct tactgtggat aatcgagctg acggccagca gaagatccgc
      301 cgtgtcgtcg atgatggcgg gggtgttgcc gctgccaacg ccgacggcgg gtttgccgct
      361 gccgtacgct gctttgacca tgcccgggcc gccggtggcg aggattaaat ccgcttcctt
      421 catggcgagg ttggtcagtt ccagactcgg cgaatcgacc cagtcgataa tgccttccgg
      481 cgcgccggcg gcaaccgccg cgtcgagcac gatctttgcc gcggcgatgg tggcgttctt
      541 tgcgcggggg tgcgggctga tgaggatgcc gttgcgggtt ttgagcgcaa gcagcgtctt
      601 aaaaatggcg gtcgaagtcg ggttggtggt cggaatcacg gcggtgatga cgccgacggg
      661 ctccgcaatg cggacgatgc cgtaggcctt gtcctcttca atgacgccgc aggttttggt
      721 gctgcggtag gcgttataaa tatattcgct ggcgtaatgg ttcttgatga ccttgtcttc
      781 caccacgccc atgccggttt cttctaccgc gagtttggcc agcgggatgc gcgcctggtt
      841 ggcggcggtt gcggccgcga ggaagattgc atccacctgt tcctgcgaga acgtggaaaa
      901 ctccttttgc gcttcgcgta agcgggtaaa cgcgcgttcc atggattcaa cgctgtcgat
      961 cggttctctt actctctgtt gtttcatacg ctccatctcc tcttttttcc tcttggcgca
     1021 ttgcaccttg attcgtttgg ccggaagtac taggttttgc cctataatcg ataacgataa
     1081 atcgattagt gctgcttccg tgttccgaac tccctgcggt gtggccgaaa tctgcgcgct
     1141 gtcgcgataa ttccggtact tttggcattc tccgcgcggg gtattccttt tgctgactat
     1201 agaataccga aaatccaacc ttgaaagaag acagaacttt ggaataccga tatatcaata
     1261 tcgatatgat aatatcgaat aacagtagcg cgatacaaaa accgcctaat tatgcctgtc
     1321 tttccagcgc aaaataaaaa agcccgcttt tgcgggcgaa atagctttcg tttacttgtt
     1381 gggaacggag gcggcggagg cgcgcagttt ttcgagaaac agcaggtcct cgcgcgtcat
     1441 gcggtagttt tcgcgccaga cgagcaggtc gctgaagctg ttgccgggca gttcgcagtg
     1501 tttttgcacc agtccgaaac aggtccagga actctcgggt tcgggagaaa ccagcgcgta
     1561 tgcgccaaag acggtgccaa gcagttccat ctggctggcg cgctcaaaga cggagatcgt
     1621 ctttctcgct tcatgaattc gggaaaactg ccttgcttcc gcaacgggca gggagggaac
     1681 cgaccagtcg ccgtagagaa tttccgtatg cggcgtaaga tcgttttcgt cgatcccgtc
     1741 tttttgcgcc agcggatgcg ttcggctcat gagcgccgtg cgggtatagc tgcggatggt
     1801 ttcgtgcttt aatccgcgct cggtgagttc gccaagaaaa tactttttat agtacgtttg
     1861 gcagcggacg atgccaaggc ggttgacttc gttggaaacg ctcttgatca cccgcatggg
     1921 cccgctctcg cggtaggaaa agccgatatc cgcttcgggg ccgatttcgt ttaccagcgc
     1981 ggagaacgcg cggctgatat agcccgcgcg cggcgcgcag aggtcaaagc ggagcttgcg
     2041 gtcgttatcg ggatgataca gcgcttccat ctcggcaacc tgcgctagaa tggcgcgcgc
     2101 gtagcttaaa aactcccgcc ccttttcagt ggggacgacg ccttttgccg tgcggtcgaa
     2161 gatggcaatg ccgatatcgt cctccagctc gcggatggcc ttgctgaggt ttggctggtt
     2221 catatagagg ttttcggcgg ccttgctgat ggagccggtc ttttccactt ccacggcgta
     2281 cttcagctgc agcaggttat tcatggggct tctcctttcg agggggagcg gacgcgctac
     2341 ttcacccgca tggcgcgccc cgcggtgcgg aacgcggcct cgtccagcgt ttctccgttc
     2401 agaacgcaga cgccgttgac gaacacgcgc tcaatgccga aggctttcgg ctgatccggg
     2461 acgccgttct tcagcgcaag ctcgtcgaaa acggtcaaat cggcgtagta tccgggcttg
     2521 atcaggccgc ggtgctccag cgagaaccgc tcggccaccg cgccggtcat cttgcggatg
     2581 gcgttcggca gggtatcgcc tttgccgttg agggaaagat gcagaaactt ggggaaacaa
     2641 tcgtagatgg cgggattctg cacgccgtgt tcttccaccc acgcgtcggt catatacagg
     2701 gagttcgggt ggcggctcag gctcgtgatg atctccggcg tgctgtaggg gcccatattg
     2761 acgcggcccg caaagtcgct ttcctcgcaa agcatgcagt aggcgtccag atcgttcatg
     2821 ccgcgctctt ttgcgatttc gtgcacggtt ttgccttcgt agtgctcgaa tcccggcccg
     2881 atatacgcaa tttgaatgtc gttgaatcca aagcccaaca gcgcgatgga ggcgtttgcc
     2941 agaacgcgga agcggaattt gttgacgaac ttgcgcttgt cctcttttga gagcgcctgg
     3001 taccaggtcg gcatgacgac ggtaatgacc gaaacgccgc agatctcgct gtaaagatcg
     3061 aacatggcgt ccacgccgtt tttgcgcatc cgctcgataa tctcaacgag ctcggccttg
     3121 catttaaacg agcttgtgcc aacaaagatt gcgtgcgagt actggagctt tacgttcagg
     3181 ccgcgggtga cctcctccag ctcgtccagc gcgcgcaaga ggtgcggccg ccccagcagc
     3241 tcagggtacg ccatggaaac ggcggagcag gcgcgcgggt gaacggtcag cgggcggttg
     3301 tagcgtgcgc agagttcggc gaccttgcgc
//
LOCUS       DAOMGY010000098         3324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30463_length_3324_cov_3.267666, whole genome shotgun sequence.
ACCESSION   DAOMGY010000098 DAOMGY010000000
VERSION     DAOMGY010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30463_length_3324_cov_3.267666"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PLR57_02305"
     CDS             <1..907
                     /locus_tag="PLR57_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQQ40316.1"
                     /translation="ILEAAANMGYRPNAIARAMITKRTGNLAFIIYGRQAPVITNPFY
                     GPILESVVNAAQRQGFSVFIVSDEEIRLPSGDLMLQKQVDGVIFASQPDLDMLEMYRR
                     NGTPVVLVNHRSELPDMVSVISDDYQGMNLVMDHLYAMGRRRIALLGGNFTEFILHRR
                     QQAYRDALARYGIAYSEALVELAEPHVADAEEGMRRILQRAGADCPDAVVCMNDTLAV
                     GAIKALRKAGKRVPDDVAVTGYDDSTICALCEPELTSVDGGKERMGEEAVRLLCELIA
                     KKTEIDSVVLDAKLRVRASSAEAVN"
     gene            960..1820
                     /locus_tag="PLR57_02310"
     CDS             960..1820
                     /locus_tag="PLR57_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQQ40317.1"
                     /translation="MESRTLQMPKKRKFRFPWRAIVAVVLLFITLFPIYWLLAMSIRN
                     NSEMAGSIPVIPQSFTMEHFIALFTEQDFGLALTNSLQVTAVSVTLSLAFGLVAAYVI
                     ARRRFRFGLKKPVTYWVLLVRVLPPVAFTIPLFIMFSKLGILHSKLPIMLACILINIP
                     LTIWFMVSFFQDLPEEIEESAKIDGATEWQLYLRVVLPLVAPGIAAIAMLSFMYAWNE
                     YTYSIIFIQTPANYTVPLKLAVLNTEDNLTQFGLVAAGGAISMIPITLFVIFAQNYLI
                     KGLSSGAVKG"
     gene            1943..3232
                     /locus_tag="PLR57_02315"
     CDS             1943..3232
                     /locus_tag="PLR57_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQQ40318.1"
                     /translation="MKKLTALLLILAMVCAAFAGCAQPAQETATEPNAADTTVTEAAA
                     EPAAANQEPTKLTLILRGGTYGDVIKAALPEFEAANNCTCEVLELAFDELHTKIALDA
                     VNAQGAYDLCMVDGSWMAEFTANNVLTDLSAMGYSFDEDIIPATTSICKVGDSIYLAP
                     FFGNVTVLLYNKQLIADAGYQPEDIDTFAEVMDIAQKTNAAGKLGFIVRGGSGDNIVS
                     DFIPHLLAYGGWVVDENNKPTVNTDAFKAAFTNYIDLYKLGDTMDKDDIVAAIDSGSA
                     AMAIGWPGWYVPSADSAASYIVIPTKLDDNDTAKNTSMYGVWTIGICNNSQNKELTLK
                     LLEYLMDQNTQLASVANGGVPCRYSSLLNADVLATHPQLETVCSALQTGVYRPVIEQW
                     SEFTNILGTEMDSVIQGTKTMDQGLADAQAALEALMS"
BASE COUNT          736 a          954 c          947 g          687 t
ORIGIN      
        1 cattctggaa gccgccgcaa acatgggtta ccggcccaac gcgattgccc gcgccatgat
       61 caccaagcgg acgggcaacc ttgcgtttat catctacggc cgtcaggcgc cggtcattac
      121 gaaccccttt tacgggccga ttctggagag cgtcgtcaac gccgcccaac ggcagggatt
      181 ttccgtgttt atcgtttcgg atgaggaaat ccgcctgccc agcggagacc tgatgctgca
      241 aaagcaagtg gacggcgtga tctttgcttc ccagccggat ttggacatgc tggagatgta
      301 ccgccgcaac ggcacgccgg tcgtgctggt caaccaccgc agcgaactgc cggatatggt
      361 cagcgtcatt tcggacgact atcagggcat gaaccttgtt atggatcacc tgtacgcaat
      421 gggtcgccgc aggattgcgc tgcttggtgg taattttacg gaatttattc tccaccgcag
      481 acagcaggct taccgggacg cgctggcgcg gtatggcata gcctacagcg aagcgctcgt
      541 ggaacttgcg gaaccgcatg ttgcggacgc tgaggaaggg atgcggcgca tcctgcaaag
      601 agcgggggcg gactgcccgg acgccgtggt ctgtatgaac gatacgctgg cggttggtgc
      661 tatcaaggcg ctgcgtaaag caggcaagcg tgtgccggac gacgttgccg tcaccggtta
      721 cgacgacagc accatctgcg cgctttgcga gccggagctt accagcgtgg acggcggcaa
      781 ggaacgcatg ggggaagaag cggtacggct tctctgcgaa ctgattgcaa agaaaacgga
      841 gatcgactcg gttgtgctgg acgccaaact cagagtgcgc gcttcttcgg cggaagctgt
      901 caactaaacg aaaatcgaga gaccgcgccg cacgtgcgca aacaactgga gggtacgaaa
      961 tggaaagcag gacgctgcaa atgccgaaga aaaggaaatt tcgcttcccg tggagagcga
     1021 ttgtagccgt tgtgctgctg tttattacgc tgtttcccat ctattggctg ctcgcaatgt
     1081 caattcgaaa caactccgag atggcgggga gcattccggt cattccgcag tcctttacga
     1141 tggagcattt tatagcgctg tttaccgagc aggacttcgg cctggcgctg accaacagtc
     1201 tgcaggttac ggctgtttct gtaacgcttt cactcgcctt cggactggtt gcggcttatg
     1261 taatcgcgcg gcggcgcttc cgcttcgggc tcaaaaagcc ggtcacctac tgggtgctgt
     1321 tggttcgcgt tttgccgccg gttgcgttta caattccgct gttcatcatg ttctccaaac
     1381 tgggcattct ccactcgaag ctcccgatca tgctggcttg catcctgatc aacattccgc
     1441 tcacgatctg gtttatggtg tcgttctttc aggatctgcc ggaggaaatt gaggagagcg
     1501 ccaagataga cggcgcgacc gagtggcagc tgtatctgcg cgtggtgctt ccgctggttg
     1561 cacccggcat tgcggcaatc gccatgctgt ctttcatgta tgcctggaac gaatatacct
     1621 acagcatcat ctttattcaa acgcccgcga actataccgt gccgctgaaa ctggcggttc
     1681 tgaacaccga ggataacctc acccagttcg gactcgttgc cgccggcggg gcgatttcga
     1741 tgatcccgat tacactgttt gttatctttg ctcagaacta cctgatcaaa ggcctgtcga
     1801 gcggggccgt gaaaggttaa caggatcgat catcgatccc gatcacacag tttgttgtct
     1861 tcgcacagaa ctacttgatg caaggctttt cgagcggggc tgtgaagggg taacaagata
     1921 aatcaaaaag gagagaaacg ctatgaaaaa actgaccgca ctgctgctga ttcttgccat
     1981 ggtttgcgcc gcctttgccg gatgcgcaca gcccgcgcag gaaacggcca cggagcccaa
     2041 cgccgctgac accaccgtta cggaggctgc cgcggaaccc gctgccgcga atcaggaacc
     2101 gaccaagctg acgctgattc tgcgcggcgg cacctatggc gacgtcatta aggccgctct
     2161 gcctgaattt gaagccgcca ataactgcac ctgcgaagtg ctcgaactgg cattcgacga
     2221 actgcacacc aagattgctc tcgacgccgt gaacgcacag ggcgcatatg acctctgcat
     2281 ggtggacggc agctggatgg cggaattcac cgccaacaac gtgctgaccg acctctccgc
     2341 gatgggctat tcgtttgacg aagatatcat tcccgccacg acttccatct gcaaagttgg
     2401 cgacagcatt tatctggctc cgttcttcgg caacgtgacc gttctgctct ataacaagca
     2461 gctgattgcg gacgccggat atcagcccga agatatcgac acattcgcgg aagtcatgga
     2521 catcgctcag aagacgaatg cggccggcaa gctcggcttt atcgtccgcg gcggttccgg
     2581 cgacaacatt gtttcggact tcatcccgca cctgctggcg tacggcggat gggttgtgga
     2641 cgagaacaac aagcccactg tgaacaccga tgcgttcaaa gcggccttca ccaactatat
     2701 cgacctctac aaactgggcg acaccatgga taaggatgat atcgttgcgg ccatcgacag
     2761 cggcagcgcc gccatggcca tcggctggcc gggctggtac gtcccctcgg cggacagcgc
     2821 agcgagctac atcgttatcc ccacaaaact ggacgataac gacaccgcaa agaacacctc
     2881 catgtacggc gtgtggacca ttggcatctg caacaacagc cagaacaagg aactgaccct
     2941 caagctgttg gagtacctga tggatcagaa cacccagctg gcttccgtcg ccaacggcgg
     3001 cgtaccctgc cgttacagca gcctgctgaa cgccgacgtg ctggcaacgc acccgcagct
     3061 cgaaaccgtc tgctccgctc tgcagaccgg cgtgtatcgc ccggtcatcg agcagtggtc
     3121 tgaattcacc aacattctcg gcaccgagat ggattccgtg attcagggaa ccaagaccat
     3181 ggatcaggga ctggccgacg cgcaggccgc gctggaagcg ctgatgtcgt aattctgctt
     3241 cagcgcagaa actcataaac taaccctgca tgctcccggc gctgcgaacg cgcgcagcgc
     3301 cgggggacag gataaacagg aaac
//
LOCUS       DAOMGY010000099         3322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30483_length_3322_cov_3.939700, whole genome shotgun sequence.
ACCESSION   DAOMGY010000099 DAOMGY010000000
VERSION     DAOMGY010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30483_length_3322_cov_3.939700"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="PLR57_02320"
     CDS             complement(<1..1327)
                     /locus_tag="PLR57_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HQQ40319.1"
                     /translation="MSEILDAVNGVVFPVWFLIGAALVFFMQGGFAMVETGFTRAKNA
                     GNIIMKNLMDFCIGTVVFVVLGFSLMMSENYLFGVIGLPDFQIFTNFAEFNFSSFVFN
                     LVFCATAATIVSGAMAERTKFSAYCIYSAIISLVIYPVEAGWVWNPQGWLFNLGYIDF
                     AGSSAIHMVGGITAFIGAAILGPRIGKYVKDAKTGKKTSRAIPGHSITLGALGVFILW
                     FGWYGFNGAAALSVEQLGSVFLTTTVAPGVATVVTMLFTWIKNGKPDVSMSLNGALAG
                     LVAITAPCANVDALGACIIGAVAGILVVLAVEFIDLKLHVDDPVGAVAVHGVNGLWGT
                     LAVGLFATDAAPGATAKGLFYGGGFAQLGIQALGVAAIAAWTIATMTGIFFLIKKTNG
                     LRVSAEEEIQGLDLPEHGLVSSYADFMITVPALDIGAAAVSKPALAVPQE"
     gene            complement(1621..2481)
                     /locus_tag="PLR57_02325"
     CDS             complement(1621..2481)
                     /locus_tag="PLR57_02325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010500735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQQ40320.1"
                     /translation="MKGKIVIVTGANSGIGKATAAQIADLGARVVLACRNKERGECAA
                     DDLAGVAGRDITLMRLDLADLDSVRAFAAAFTERYDHLDALINNAGILGRWRAETKQG
                     FEMSFGVNYLGHFLLTMLLLPLLQKAEQGRIVMMSSMAHAWVDVRFDDLNYTHGYNRF
                     LAYGHSKLCNLLFARVLADKLKQKGSRVTINAVHPGIVATDIVVNRKTGAFRWVASLM
                     RLFFTSSEEGAETSVYLACDPDAAKYSGEYFYKKHVEPSSPASKNLESAYRLYNISLR
                     LAGLESDPLC"
     gene            2746..>3322
                     /locus_tag="PLR57_02330"
     CDS             2746..>3322
                     /locus_tag="PLR57_02330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ40321.1"
                     /translation="MFLADGWSDFRVLDAGNGMKLERWKDITLLRPDPQAVWPMAVPA
                     ADAVYLRSETGGGHWEFTRQLPEQWKIQYRDLTFLVRPTGFKHTGLFPEQAVNWDWMR
                     GELEQWKQRHDRAPRVLNLFAYTGGASVACAAAGAEVVHVDAAKGMVAWAKNNAEASG
                     LNKAVIRYIVDDCVKFVKREQRVSKVVEDVDE"
BASE COUNT          759 a          918 c          997 g          648 t
ORIGIN      
        1 cttcctgcgg tacggcgagg gcgggcttgg acactgccgc agcgccgatg tcgagggcgg
       61 gaaccgtaat cataaagtcc gcatagctgc tgaccagacc atgttccggc aggtcgagac
      121 cctgaatttc ttcctcggcg gaaacgcgga ggccgttggt ctttttaatc aggaagaaga
      181 tgccggtcat tgtggcaatg gtccaggccg cgatggccgc cacgccgagc gcctgaatgc
      241 cgagctgcgc aaacccgccg ccgtagaaga gacccttggc cgttgcgccg ggagccgcat
      301 cggttgcgaa caggccgact gcaagcgtgc cccaaagacc gttgacgcca tggaccgcaa
      361 ccgcgccgac cggatcgtcc acgtgcagct tgaggtcgat gaactcaact gccaacacca
      421 cgaggatacc ggcgaccgcg ccgatgatgc aggcgccgag cgcgtcaacg ttcgcgcagg
      481 gagccgtaat tgcaacgagg cctgcgagcg cgccgttgag agacatagag acgtccggct
      541 tgccgttctt gatccaagta aacagcatcg tgacaacggt tgccacgccg ggagcgaccg
      601 tggtggtcag aaagacggaa ccgagctgct ctacgctcag agctgccgcg ccgttaaagc
      661 cgtaccagcc gaaccagagg atgaatacgc cgagcgcgcc gagcgtaatc gagtgaccgg
      721 gaatcgcgcg ggacgtcttt ttgccggtct tagcgtcctt tacgtatttg ccgatacgcg
      781 ggccgaggat agccgcgccg atgaaggccg tgatgccgcc caccatgtgg atggcggaag
      841 aacctgcaaa atcgatgtag ccgaggttga agagccagcc ctgcgggttc cagacccagc
      901 cagcctctac ggggtagatg acgaggctga tgatcgcgct gtagatgcag taggccgaga
      961 acttcgtgcg ttccgccatc gcgccggaga cgatcgtcgc cgccgtggcg cagaacacaa
     1021 ggttaaagac gaagctggaa aaattgaact ccgcgaagtt cgtgaaaatt tggaagtccg
     1081 gcaggccgat gacgccgaac aggtagtttt ccgacatcat caggctgaaa cccagcacga
     1141 cgaacaccac ggtgccgatg cagaaatcca tgaggttttt catgatgatg ttgccggcgt
     1201 tcttcgcgcg agtgaacccg gtttccacca ttgcaaaacc gccctgcata aagaatacga
     1261 gcgctgctcc aatcagaaac caaactggga atacgacgcc gtttacggca tctaaaatct
     1321 cggacattac gatacctccc caaaaaacaa aataggccaa aaggcgccgt ctgtagaaac
     1381 agacagcgcc cttgcttgac cttgcaagta atataacgga ttgcttttca aaaaacaaga
     1441 ggaattttgg aatttttgta aaaataatat ataaacagtt ataaagcgtg atataaaatt
     1501 atatagtaga gaatcgggca agtcttacgc aaataacgag tgcttgcgag cgaaaaacgg
     1561 gtgattttgc ggaaaagttc gccgaaacgc ccaatgattg cgcagggcaa attgccattt
     1621 ttagcatagc gggtcgcttt ccaaccccgc gagccgcagg ctgatgttgt agagccggta
     1681 ggcgctttcc aggtttttgg aagccggact ggaaggctcg acgtgctttt tgtagaagta
     1741 ttctccgctg tattttgccg catccgggtc gcaggcaagg tagacgctcg tctccgcgcc
     1801 ttcctcggag gaagtgaaaa acagccgcat gagcgacgcc acccagcgga acgcgccggt
     1861 cttgcggttg accacgatat cggttgccac gatgccgggg tgtacggcgt tgatcgtaac
     1921 cctgctgcct ttttgcttga gcttgtcggc cagaacgcgc gcaaacagga ggttgcagag
     1981 cttgctgtgg ccgtacgcca gaaatcggtt gtagccgtgc gtatagttga ggtcgtcaaa
     2041 gcggacgtcc acccaggcgt gcgccatgct gctcatcatt acgattcgcc cctgctcagc
     2101 cttctgcagc agcggtaata gcagcatcgt cagcagaaag tggccgaggt agttgacgcc
     2161 gaagctcatt tcaaacccct gcttggtttc cgcgcgccat ctgccgagga tgcccgcgtt
     2221 gttgatgagc gcgtccagat ggtcataccg ctccgtaaac gccgccgcga acgcgcgtac
     2281 gctgtccaga tcggcaagat cgagccgcat gagcgtgatg tcgcgccccg caacgccggc
     2341 gagatcgtcc gccgcgcatt ccccgcgctc cttattgcgg caggcgagaa cgacgcgcgc
     2401 gccaaggtcc gcaatctgcg ccgcggtcgc ctttccgatg ccggaatttg cgcccgtaac
     2461 gatgacgatt ttccccttca tatccccgtt tatttttcca tccatggcaa aaagtatagc
     2521 gaacggggca tgaaaaagca acttgcgcgc gccgcgcggg aaatcgaact tgtcaaaaac
     2581 ccaactagac gaatatactg attaagaact acgagggctc tgccctcgcg ctcccgcaaa
     2641 aggggattcc ccttttgaaa ccctacgctt tatatggttg gagtattttg acagcattgg
     2701 ggttttaagg ggtatggggg tgaaaccccc aaaaagagag gttttatgtt tttagccgat
     2761 ggatggagcg actttcgcgt gctggacgcg ggaaatggta tgaagctgga gcgctggaag
     2821 gatatcaccc tcctgcgtcc cgacccgcag gccgtctggc ctatggccgt gcccgcggcg
     2881 gatgctgtct acctgcgctc cgaaaccggc ggcgggcact gggagtttac cagacagctg
     2941 cccgagcagt ggaagataca gtaccgcgat ttgacgtttc tcgtccgccc gacgggtttt
     3001 aagcacaccg gcctctttcc cgaacaggcg gtcaactggg actggatgcg cggggaactg
     3061 gaacaatgga aacagcgcca tgaccgcgcg ccgagggtgc tcaacctctt tgcgtatacc
     3121 ggcggggcga gcgttgcctg cgcggcggcg ggcgcggaag tggtgcatgt ggacgcggca
     3181 aagggcatgg tcgcctgggc gaagaacaac gccgaggcga gcgggctgaa caaagccgtg
     3241 attcggtata tcgttgacga ctgcgtcaag ttcgtcaagc gcgagcagcg ggtttcgaag
     3301 gtggtagagg acgttgatga ac
//
LOCUS       DAOMGY010000100         3317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30556_length_3317_cov_2.919681, whole genome shotgun sequence.
ACCESSION   DAOMGY010000100 DAOMGY010000000
VERSION     DAOMGY010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30556_length_3317_cov_2.919681"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PLR57_02335"
     CDS             <1..962
                     /locus_tag="PLR57_02335"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HQQ40322.1"
                     /translation="YDIIIVNDGSDKDRLPIFESLEPYAKVLTHEVNRGKGAALKTAL
                     SYIYEQYPADEGVVTIDADGQHLPADVIRVSEAWEQAPERLVLGSREFTGNVPFKSRA
                     GNAITRCVFAASTGVKVFDTQTGLRAFGVFRIPMMMEMKGDRYEYEINVLLYATKHRI
                     PIQEVVIQTVYIEDNKSSHFNPVRDAWRIYKMILLFVASSLLAMGIDYAALLTLSAAT
                     KSLPQSLLISVVGARLISSLVNYFINCKLVFEHRSLASIIRYYLLVAGILAIDYGMLY
                     VTTQWITLPVAKIIVEIALYPLSFYIQRKYVFPPQTEDIGADN"
     gene            1021..2073
                     /locus_tag="PLR57_02340"
     CDS             1021..2073
                     /locus_tag="PLR57_02340"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HQQ40323.1"
                     /translation="MIKPAKSPAKRLITAIVIDLLVLGALLYAYSYVTFLRVLDYTPK
                     ALNSPTPAAAATPSPDENAALANSPSSEEAVSPSPTPVDTGLLGGNYAEKFTSGEVEQ
                     TEDSYRSANVSIEMSQRTAGTTEKPIVYYVADIYIKNISSFRTAVAEEYKDQNESDIK
                     NSMPALQLSQLAGAIVSINGDSFAWHDGLAVRNSIEWTRDLPVYGDICVLYKDGTMET
                     YAQKDVKNALIDEIYAKDPYHIWTFGPQLLVDGQVPANFTRSKANPLSAVGYAEPGHY
                     YFILVDGRQKGYSWGMTLEELAQVFYDLGCKVAFNLDGGDTAVMTYNGAWRSQPENSS
                     PRNTSDILFICEPDAQ"
     gene            2101..2394
                     /locus_tag="PLR57_02345"
     CDS             2101..2394
                     /locus_tag="PLR57_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40324.1"
                     /translation="MKRIITRVLPHITIILGLMTLVFFVVDGFNPIMAFMSNELSKKI
                     FAVLAVCGIASSVMLIARQWQEDDRRARKLARQQRRQELLAEETEKSFEEEAR"
     gene            complement(2656..3317)
                     /gene="metK"
                     /locus_tag="PLR57_02350"
                     /pseudo
     CDS             complement(2656..3317)
                     /gene="metK"
                     /locus_tag="PLR57_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253520.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
BASE COUNT          696 a         1004 c          913 g          704 t
ORIGIN      
        1 actacgatat cattattgtc aacgacggca gcgacaagga ccggctgccg attttcgagt
       61 ccctggaacc ctacgcaaag gttttaacgc acgaagtcaa ccgcggcaaa ggcgccgctc
      121 tgaaaaccgc tctttcgtat atctatgagc agtaccccgc ggacgagggc gtcgtcacca
      181 tagacgccga cggacagcac ctgcccgcgg acgtcatccg cgtcagcgaa gcctgggagc
      241 aggcgcccga acgccttgtt ctgggcagca gggaatttac gggcaacgtg ccgttcaaaa
      301 gccgcgcggg caacgccatt acgcgctgcg tgttcgccgc gtccacgggc gtaaaggtgt
      361 ttgatacgca aaccggcctt cgcgcgttcg gcgtgtttcg catccccatg atgatggaga
      421 tgaaaggcga ccggtacgag tacgaaatca acgtgctcct ctacgcgacc aagcaccgga
      481 ttccgattca ggaagtcgta attcaaacgg tgtatattga ggacaacaaa tcctcgcact
      541 tcaaccccgt gcgcgacgca tggcgcatct acaagatgat tctcctgttc gtggcttcgt
      601 cgctccttgc catgggcatt gattatgccg ccttgctgac gctgtctgcg gcaacgaagt
      661 ccctgccgca gtcgctgctc atcagcgttg tgggcgcgcg cctgatcagt tcgctggtca
      721 actattttat caactgcaag ctcgtgtttg agcaccgcag cctcgccagc atcattcgct
      781 attacctgct ggttgcgggg attctcgcga ttgattacgg aatgctgtat gtgaccacgc
      841 agtggattac gctgcccgtt gccaagatta tcgttgaaat cgcgctctac ccgctgagct
      901 tttacattca gcgtaaatat gttttcccgc cgcagaccga agacatcggc gcggataact
      961 gattttttcg ccgcggctta ttaccgcggc attcctgcct aaggaggtat ttcttccgtt
     1021 atgataaaac ccgccaagag tcccgcaaag cggctgatta cggccattgt aatcgacctt
     1081 ctggtgctcg gcgctttgct ttacgcgtat tcttacgtta cgttccttcg cgtgctcgac
     1141 tatacgccaa aagcgcttaa cagcccgact cccgcggctg cggcaacgcc ttctccggac
     1201 gaaaacgcgg ctctcgccaa ctcgccttcg agcgaagaag ctgtttcgcc ttcgcccact
     1261 cccgtggaca ccggcctgct ggggggaaat tatgctgaaa agtttaccag cggcgaagtg
     1321 gagcaaaccg aggattccta ccgcagcgcg aacgtctcta ttgaaatgtc ccaaaggact
     1381 gcgggtacga cggaaaagcc gatcgtctac tacgttgcgg atatttacat caagaatatt
     1441 tcgagcttcc gcacagctgt ggcggaagag tataaagatc agaacgaaag cgacattaaa
     1501 aattcgatgc ccgcgcttca gctttcccag ctggcgggcg ccatcgtttc gatcaacggc
     1561 gacagctttg cctggcatga tggcctcgcc gtgcgcaaca gcatagaatg gacaagggac
     1621 ctgccggtct acggcgatat ctgcgtgctc tataaagacg gcaccatgga aacctacgcc
     1681 cagaaggacg ttaaaaacgc gctgatcgac gaaatctacg caaaggatcc ctaccatatc
     1741 tggacgttcg gcccgcagct gctcgttgac gggcaggtgc ctgcaaactt tacgcgctcc
     1801 aaggcgaacc cgctctccgc cgtagggtat gccgaaccgg ggcattacta ctttatcctc
     1861 gtggacgggc ggcaaaaggg gtattcctgg ggcatgacgc tggaagaact tgcgcaggtg
     1921 ttctacgatc tcggctgcaa agtcgcgttc aaccttgacg gcggcgacac cgcggtgatg
     1981 acctacaacg gcgcatggcg cagccagccg gaaaacagca gcccgcgcaa cacgagcgac
     2041 atactcttca tttgtgaacc ggatgcgcag taaagcgtgc agtaaggagg agactgaaag
     2101 atgaaacgaa tcattacccg cgtgctgccg catataacga ttatcttagg cctgatgacc
     2161 cttgtgtttt tcgtggttga cggctttaac ccgattatgg cttttatgag caacgaactt
     2221 tccaaaaaga tcttcgccgt gctcgccgtc tgcggcatcg cctcgtcggt catgctcatt
     2281 gcccgccagt ggcaggagga cgaccgccgc gcgcgcaagc tcgcccgcca gcagcgcagg
     2341 caggaactgc tcgccgagga aaccgagaaa tcctttgaag aagaagcccg ctgacacgca
     2401 ggcaacgcaa acgccgccct tgacgggcgg cgttttatat gtcttttttc ttggggcttc
     2461 accccatctc agaacgaagc cttcttgggg cttcgcccca aaccctgact caggggtgtt
     2521 cacccctgag aaccccctct ttggcaggcg attcgatacg gggtttccaa aggggtgaac
     2581 acccctttgg caggagctcg agggctggcc ctcgaatcat tcccctgctc gagggcagcg
     2641 ccctcgaaag aagccctact taattgcaat gcgcagcgct tcggcgcggt cggtctgctc
     2701 ccaaggcagg ttcaaatcgc tcctgccaaa gtggccgtaa gccgccgtct ggcggtagat
     2761 cggacggcgc agcttgagct gctcgatgat ggccgcgggg cggaagtcga acacgctcaa
     2821 aatcgccttc tcaatcgcct cttccgaata cgcgcccgtg ccgaacgtat ccacgcagat
     2881 ggagacggga cgcgccacgc cgattgcgta cgccagctgg atttcgcact ttttcgccag
     2941 ccccgccgca acgacgttct ttgcggcgta ccgcgccata taggccgcgc tgcggtcgac
     3001 ttttgtcgga tccttgccgc tgaagcagcc gccgccgtgc cgcgcgtagc cgccgtaggt
     3061 atccacaatg atcttgcgcc cggtcaggcc ggaatcgccc gtcggcccgc cgatgacgaa
     3121 cctgccggtc gggttgacgt agatcttcgt ctttacatcc accagcgcag tcggcagcgt
     3181 ggggcggatc accttttctt cgatgtcccg ccggagtatg gccatatcca catcgtcatg
     3241 ctgcgcgctg atgacgaccg tgtccacgcg gagcggcttc gccttcgtac tcaatggtca
     3301 cctggctctt gccgtcc
//
LOCUS       DAOMGY010000101         3317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30558_length_3317_cov_2.742489, whole genome shotgun sequence.
ACCESSION   DAOMGY010000101 DAOMGY010000000
VERSION     DAOMGY010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30558_length_3317_cov_2.742489"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PLR57_02355"
     CDS             complement(<1..670)
                     /locus_tag="PLR57_02355"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HQQ40325.1"
                     /translation="MCFLKRAALIVTAAALLVSLPLMNAAADTGEGGEGASNRQVHLF
                     SQQPVESSFDVSGFRKVAESDKAELWLNEQFGTLRIVALDTGYVWGALPLEDAEGINK
                     SWNGYGNSIAAIECFDDSASVKRLSFGKDAQVNYEITPEGLLCRADFGEAGIRFSVLI
                     KLEGERINFSLVPGSLEEGLNGSTYTIKSLSFLPYLGASLNDNIDGYLFIPDGSGALI
                     RFQKA"
     gene            complement(657..2705)
                     /locus_tag="PLR57_02360"
     CDS             complement(657..2705)
                     /locus_tag="PLR57_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ40326.1"
                     /translation="MKNNRKRLPLLALALLTAFCVCIPSTLAMEGTTYTRALSADLTR
                     AVRTQEAYIPAGTFFADLGMASPEDLFYSDGKIYIADSGNHRVLVCSPQGSLFSVIGE
                     ETLKKPTGIAVADDGTIYVADYSAEAVVVFSADGTEQMRIVRPTDVYFGSGVYRPRKV
                     ALDRFGNLYVVSEGTNEGIIQFNLKGEFGGFFGANKTPPLTVREWFQKTFYTDEQKSK
                     LTFRTPASIVSLDVSQNGLVYSATQGERFSAVKALNMAGYNILPDDIPMYSMNYVDVA
                     VTGTGAFYGVTDEGVIDEFSEDGLLLVFFGGRAATTDRNGLTAVASAIDTDPAGNLYV
                     LDKERGTVQIWYPTDYAALLHRASNTFRAGQYEESLSCWQEILRMNPATYMAHEGVAS
                     ALFQLGRYQEAAAHARVISDRSLYSSCFWKLRSAWINEHIQSIVVICFGLMVVLIVLH
                     QIRKKHDFLLPLKRAWAAQKRKHPMLGRLIDDPLYQMRHPIDGVYYLKIRQRGSFGAA
                     VVLYIAAFAVYLLCRAGTSFVFGGGFWLYSSPVAVSLIVLVPAGLFVVGSYLISSIND
                     GEGTLRQSFIAIGYSLSAFILFWPILAGLSWLFTYAELFQYQALQTLVLGYTGIMVFL
                     AVKETHAYTPKKTVANLLLTLAFMVIAVLAAIILYILWKELFSFVLQILEEVKYRVFS
                     "
     gene            complement(2726..>3317)
                     /locus_tag="PLR57_02365"
     CDS             complement(2726..>3317)
                     /locus_tag="PLR57_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQQ40327.1"
                     /translation="AYCMSKKQYKLKNTLFAVNEAALMFVSVAVAIPRYFIVSKLGLT
                     DSFWAHVIPLLAMPVGLYLVKQFVDDLPDSMIEAARIDGADDYTILWRIVIPNIAPAL
                     ATVAILAFQASWNSVEASNYFISNEALKSFSFYMTTLTANAGNTSVMVGQLSGNAVAG
                     LGMQAAAVLIMFIPNLILFILLQSRVMNTMSHSGMK"
BASE COUNT          742 a          982 c          946 g          647 t
ORIGIN      
        1 gcgccttctg gaaccgaatc agcgcgccgc ttccgtccgg aataaagagg tagccgtcga
       61 tattgtcgtt gagcgaagct ccaaggtatg gcagaaacga aagcgacttg atcgtatacg
      121 tgcttccgtt caggccttcc tccagactgc ccggcacaag cgaaaagttg atccgttcgc
      181 cttccagttt gatcagcacg gaaaagcgaa tgcccgcttc gccgaagtcc gcccggcaga
      241 gcaggccttc cggcgtaatt tcgtagttga cctgcgcgtc cttgccaaag ctcagacgtt
      301 tgaccgatgc cgaatcgtca aagcactcga tggcggcgat ggagttgccg taaccgttcc
      361 aggacttgtt gatcccctcc gcgtcctcca gcggaagcgc gccccaaacg taaccggtgt
      421 caagcgcaac aatccggagt gtgccaaatt gctcgttgag ccagagctct gctttatcgc
      481 tttcggctac cttgcgaaag ccggaaacgt caaagctgct ctccacgggc tgctgggaaa
      541 acaggtgaac ctgccggttg gaagcgcctt cgcccccttc tcccgtgtcc gccgcagcgt
      601 tcatcagcgg cagagacaca agcagcgccg ccgcggtgac aatcagcgcc gcgcgcttaa
      661 gaaaacacac ggtacttcac ctcctcgaga atctgcagga caaagctgaa aagctccttc
      721 cacagaatgt acagtatgat ggccgcgagc accgcgataa ccataaacgc aagcgtcaaa
      781 agcaggtttg caaccgtctt tttcggcgtg taagcgtgcg tctccttgac ggccagaaaa
      841 accataattc cggtatagcc gagaacgagc gtctgcagcg cctgatactg aaacagctcg
      901 gcataggtaa acagccagct cagccccgca aggatcggcc agaacaggat aaacgccgac
      961 agcgaatacc cgatggcgat gaacgactgc ctgagcgttc cctcgccgtc gttgatagag
     1021 ctgatcaggt aactgccaac cacgaacagc cccgcgggca ccagcacgat caaactgacc
     1081 gcaaccggac tgctgtagag ccagaacccg ccgccgaaca caaacgacgt gcccgcccgg
     1141 caaagcagat agaccgcaaa cgccgcgata tacagcacca ccgccgcgcc aaagctaccg
     1201 cgctgccgga ttttgaggta gtacacgccg tcgatcgggt ggcgcatctg gtagagcgga
     1261 tcgtcgatga gccgccccag catgggatgc tttcgctttt gggccgccca ggcgcgcttc
     1321 aacggcagga gaaaatcgtg ctttttgcgt atctggtgca gaacgatcag caccaccata
     1381 aggccaaagc agattacgac gatcgactga atatgctcgt tgatccaggc gctgcgcagt
     1441 ttccagaagc aggaagagta gagcgaccgg tcgctgatga cgcgggcgtg cgccgccgct
     1501 tcctgatagc gccccagctg aaacagcgcg gaagcgacgc cttcgtgcgc catatacgtt
     1561 gccgggttca tgcgcagaat ttcctgccag caggacaggc tctcttcgta ctgccccgcg
     1621 cggaaggtat tgctggcgcg gtgcaggagc gccgcgtagt cggtcggata ccaaatctgt
     1681 accgtgccgc gctccttatc gagcacgtag agattgcccg ccgggtcggt atcgatggcg
     1741 gaggcaaccg cggtgagccc gttgcggtct gtggttgcgg cgcggccgcc aaagaacacc
     1801 aaaagcaggc cgtcttcgct gaactcgtcg atgacgccct cgtcggtcac gccgtaaaac
     1861 gcgccggtgc cggtaaccgc aacgtccaca tagttcatcg agtacatcgg gatatcgtcc
     1921 ggcaggatat tatagcccgc catgttcagc gctttgacag cgctgaagcg ttcgccctgc
     1981 gtagcggagt acacgagccc gttttgagaa acgtccagag aaacgatgct ggccggtgtg
     2041 cggaaggtga gcttgctctt ctgctcgtcg gtataaaacg tcttttgaaa ccactcgcgc
     2101 actgtcagcg gcggtgtttt atttgcgccg aagaacccgc cgaactcgcc tttgagattg
     2161 aattggatga tgccttcgtt ggtgccttcg ctcacgacgt agaggttgcc gaagcgatcc
     2221 agcgcaactt ttctcgggcg gtaaacgccg ctgccaaagt acacgtcggt cgggcgcaca
     2281 atgcgcattt gttcagtacc atcggcagaa aacacgacca ccgcctcggc ggaatagtcc
     2341 gcaacgtaaa tcgtcccgtc gtccgcaacg gcaatgcccg tcggcttttt gagtgtttct
     2401 tcgccgatca cggagaacag gctcccctgc gggctgcaga ccagcacgcg gtgattgccg
     2461 ctgtcggcga tatagatttt tccgtcgctg tagaacaaat cctccggact cgccatgcca
     2521 aggtcggcaa aaaacgtacc cgccggaata tacgcttcct gcgtgcgcac ggcgcgcgtc
     2581 aaatccgccg agagagcgcg cgtgtaggtc gttccctcca tggcaagcgt ggacgggatg
     2641 cacacgcaga atgctgtcag taaggcgaga gcgaggagcg gcaggcgttt tctgttgttt
     2701 ttcatgcgcg gtatcctcct ttgccttact tcatgccgga gtgcgacatg gtgttcatga
     2761 cgcgcgactg cagcaggata aacagaatca ggttcggaat aaacataatc agcaccgccg
     2821 ccgcctgcat gccgagaccc gcaacggcgt ttccgctgag ctggccgacc atgacgctgg
     2881 tgttgcccgc gttggccgtc agcgttgtca tataaaacga aaagctcttg agcgcttcgt
     2941 tgctgataaa gtagttggaa gcttccacgc tgttccagga agcctgaaac gcgagaatgg
     3001 caacggttgc cagcgcgggc gcaatgttgg gaatgacgat tcgccagaga atcgtatagt
     3061 cgtccgcgcc gtcgatgcgc gccgcttcga tcatagaatc cggcaggtcg tctacaaact
     3121 gcttgacgag atacagcccc accggcatgg ctagcagcgg aatcacatgt gcccagaagc
     3181 tgtccgtcag cccgagtttg gaaacgataa agtaacgcgg aatggccacc gcgaccgaaa
     3241 cgaacatgag cgccgcttcg tttacggcaa acagcgtgtt tttgagcttg tactgctttt
     3301 tggacatgca gtaggcc
//
LOCUS       DAOMGY010000102         3313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30612_length_3313_cov_3.015040, whole genome shotgun sequence.
ACCESSION   DAOMGY010000102 DAOMGY010000000
VERSION     DAOMGY010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30612_length_3313_cov_3.015040"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLR57_02370"
     CDS             complement(<1..835)
                     /locus_tag="PLR57_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HQQ40328.1"
                     /translation="MAKSNPFFRVDSGDDPKLKEPELENQPLLKERDKPSSFFLAVLF
                     TSLKMLILAFIVVCFIGFGLLVGVVKAYVETTPSLDVAQLTISDRTSYLYDMNGDLLT
                     TIADVEYRDWVDIENIPDMLKNAFISVEDVRFYKHSGVDFKRLFSAALEILGNSNSTG
                     GSTITQQLIKNKILGSQRNYKRKIQEAYLALEVETIITKDQILEAYLNDIPLGESNYG
                     VKSAAMDYFGKELSELTVRECAMLAGLPQAPYRYDPRKNMYQRDQMEVTDERTNQVIL
                     VT"
     gene            1088..1164
                     /locus_tag="PLR57_02375"
     tRNA            1088..1164
                     /locus_tag="PLR57_02375"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1122..1124,aa:Ala,seq:ggc)
     gene            complement(1214..2026)
                     /locus_tag="PLR57_02380"
     CDS             complement(1214..2026)
                     /locus_tag="PLR57_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40329.1"
                     /translation="MESTWNITRYSGHCYTPEQTLAIRNTTGHDALTPDDVDAALQER
                     EDLSLLQASRLNQASFEYLISRYGDHLRTIYLFSSPDIRDFSPLSALPLLENVLIYWN
                     RKAETLWDMSNNTGLRGIQISASKRLLLGLDALATAPHLEEIRLNGDILTNHSIRDFS
                     VFERCGSLKRLSLLGLNVLDGTMEPLFRLANLEIFDFDPSFFTLDQIALLKAKLPATT
                     GMSLCAFQQDGEDKVRINGKRMPTLKLPQQQKRRDEYVERFEELVREYRRQA"
     gene            complement(2067..2741)
                     /locus_tag="PLR57_02385"
     CDS             complement(2067..2741)
                     /locus_tag="PLR57_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HQQ40330.1"
                     /translation="MNRIEQKLDFLRRVEESEEPLQAEETVYLIELLSAKNADVRCRA
                     AISAGICFLPEFEKPLLQLLNDKKRIIRVNACDSLSNSANMAVAFELVPLMQSRYRLE
                     RGYAVLSYSDIALNAGAPKEETINVLRPYMEAEKDDWAKAFGVEALAQLGDYGALEHL
                     YRLIGSKDAHARRAALKCAARFVAYMDRDMLETTLIRQLEQERDETIKGLISSLLEKV
                     GESENV"
     gene            complement(2770..>3313)
                     /locus_tag="PLR57_02390"
     CDS             complement(2770..>3313)
                     /locus_tag="PLR57_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40331.1"
                     /translation="PDFVSARGEVGLEVTRAVIEEWARQEAAVGKKFGKDISDDMVRQ
                     NSKTGTNDALLTRLDLTRDLSNGDTGGDPLDAEIHIGRAIERIEGKTKLLPDYQPCDE
                     YWLYLYSETLHLNADDISRIAQHVEQMPQKQKFRMIFIKVGGSLYVLESGKKPREIAL
                     NGRVLRRIHRRAHWKSMLIR"
BASE COUNT          752 a          902 c          798 g          861 t
ORIGIN      
        1 ccgtcacgag gatgacctgg ttggtgcgct cgtcggtaac ttccatctgg tcgcgctgat
       61 acatgttctt gcgcgggtcg tagcggtacg gcgcctgcgg gagccccgca agcatggcgc
      121 actcacgaac agtcagttcg gaaagctcct tgccaaagta gtccatggcg gcggatttca
      181 cgccgtagtt cgactcgccc agcggaatat cgttcaggta cgcttccaga atctgatctt
      241 tcgtgatgat ggtttccact tcgagagcaa ggtacgcttc ctgaatcttg cgcttgtagt
      301 tgcgctggga accgaggatt ttgtttttaa taagctgctg ggtaatcgtg ctgccgccgg
      361 tggaattgct gttgcccaag atttccagcg cggcggaaaa cagtcgctta aagtccacgc
      421 cggaatgctt gtaaaagcgc acgtcctcaa cggagatgaa cgcgtttttg agcatgtcgg
      481 gaatgttttc tatatccacc cagtcacggt attctacgtc cgcgatggtc gtaagcaggt
      541 cgccgttcat atcgtagagg tagctcgtgc ggtcggaaat cgtcagctgc gccacgtcca
      601 gagaaggcgt cgtttcaaca tatgccttga caacgccgac caaaaggccg aaaccgataa
      661 agcaaacgac gatgaacgcc agaatcaaca ttttaagcga cgtaaacaaa accgccagaa
      721 agaaagaact cggtttgtcg cgttccttta ataacggctg gttttccagc tccggttctt
      781 tcagttttgg gtcgtctccc gaatccactc gaaaaaacgg attgcttttt gccattcgtc
      841 tgcacctcaa tgaaatctta ggtattatag catacggttg gtttctttat gcgttttgcc
      901 gcaaaaagtt catactttcc gcgtttttcg acttgcttta tatacaaaaa agtgagacga
      961 tatcgagcgc acagaacaaa gcgaggcgct ttgcacaaac aaaattcaca ctatattatt
     1021 cgcccaaaat tcgaaaattt agtctttaca aaacaccggc tttccactat aatagcacat
     1081 gcggctcggg gctttagcga agttggctat cgcgctacac tggcagtgta gagatcaccg
     1141 gttcgaatcc ggtaagctcc accaaacgaa aactcgcgga caaatcgtcc gcgagttttt
     1201 ttgttttctc cagtcaagcc tgccgccggt actccctaac cagctcttca aatctctcca
     1261 catattcatc ccgtcgtttc tgttgttggg gcagtttcag cgttggcatt cgcttgccgt
     1321 ttatgcgcac tttgtcttcc ccatcctgct gaaaggcgca gagagacatg cccgtagtcg
     1381 cgggcaattt cgctttgaga agcgcaattt ggtccagcgt aaagaacgat ggatcaaagt
     1441 cgaaaatctc aagattcgca agcctgaaca gcggttccat cgttccatcc agcacgttca
     1501 gtccaagcag tgaaagacgt ttcagactgc cgcatcgttc gaatacggaa aaatcccgaa
     1561 tcgagtgatt ggttaaaatg tctccgttca gtcgtatctc ttcgagatgc ggcgcagtcg
     1621 ccaacgcatc cagcccaagc agaagacgtt tcgacgcgct gatctggatt cctctcaagc
     1681 cggtgttgtt actcatgtcc caaagcgttt cagctttccg gttccagtag atcagcacat
     1741 tttccagcag cggcagcgcg gacaacggcg aaaaatcccg tatgtcggga ctggagaaaa
     1801 ggtatatcgt tctgagatga tccccgtagc gcgaaatcag atattcaaaa cttgcttgat
     1861 tcagcctgct cgcctgcaaa agcgacaaat cttcccgctc ctgcaaagcc gcgtccacat
     1921 cgtcaggcgt gagtgcatca tgccctgtag tgtttctgat cgcaagagtc tgctccggcg
     1981 tgtagcaatg tcccgaatag cgcgtgatgt tccaggtgct ctccatatgt tattccttca
     2041 gccggttgat cggcttttag tcagtttcat acgttttcag attcgccgac cttttccaaa
     2101 agactcgata tcagaccctt aatcgtctcg tcgcgttcct gttcaagctg cctgatgagt
     2161 gtggtttcca acatatcgcg atccatgtat gccacaaacc gagccgcgca ctttaaggcc
     2221 gcgcgtctcg cgtgggcatc ttttgagccg atcaaacggt acaaatgctc caacgctcca
     2281 tagtcgccaa gctgtgccag cgcttcaact ccgaacgctt tcgcccaatc gtccttttcc
     2341 gcttccatat acgggcgcag aacgttaatt gtttcctctt tgggcgctcc tgcgtttaat
     2401 gcaatgtcgg aataactcag cacggcgtac ccgcgttcca atcgatacct gctctgcatc
     2461 agcgggacca gctcgaaagc aaccgccata ttggcgctgt tgctcagaga atcacaggcg
     2521 ttgacacgga tgattctctt tttgtcattg agaagctgta gcaacggctt ttcgaattcc
     2581 ggcaaaaaac aaatccctgc tgatatggcg gcgcgacacc gcacatcggc gttttttgcg
     2641 cttagaagtt cgatcaggta cacagtctct tccgcctgca gtggttcttc gctttcttcc
     2701 acgcgccgca gaaaatcaag tttttgttcg atgcgattca tatatgatcc tcaatcagta
     2761 aaaaactatt tagcgaatca gcatcgattt ccagtgcgca cgccggtgaa tgcgccgcag
     2821 taccctgccg ttcagcgcaa tttctcttgg cttttttccg ctttcgagca catacaggct
     2881 gccgccaact ttaataaaga tcatgcgaaa cttctgtttc tgcggcattt gttcgacgtg
     2941 ctgtgcaatc ctcgatatat cgtccgcatt gagatgcaag gtttcgctgt agaggtagag
     3001 ccaatattca tcacagggct gatagtccgg caggagcttg gtttttcctt ctatgcgctc
     3061 gatggcgcgg ccgatgtgaa tctccgcatc aagcggatcg ccgcccgtat caccattcga
     3121 aaggtcgcgt gtcaggtcaa ggcgggttaa cagcgcgtcg ttcgtacccg tcttgctatt
     3181 ctggcgaacc atgtcgtccg agatgtcttt gccaaacttc ttgccaacgg ccgcctcctg
     3241 ccgcgcccat tcttcgatca ccgcgcgggt cacttccagg ccgacttcgc cgcgcgcgga
     3301 gacaaagtcc ggg
//
LOCUS       DAOMGY010000103         3304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30732_length_3304_cov_2.562327, whole genome shotgun sequence.
ACCESSION   DAOMGY010000103 DAOMGY010000000
VERSION     DAOMGY010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30732_length_3304_cov_2.562327"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="PLR57_02395"
     CDS             complement(<1..1427)
                     /locus_tag="PLR57_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40332.1"
                     /translation="MAGRLNRKKTMLALRSLLMGLVVLALFFGLPSRAFAEGEDDTSA
                     GDGDVTATVPLAATEGETTIVEHTQSGESGNQLDEIDADPDNLTFENDLPSVTTTTIE
                     TLDGTGTVTDTTVTNTLTDTGEAVELTTPTTVTPDNTQVNETSDTRTTVTISASENAI
                     QKALDKVLAELAPDSTSATIRVAAGTYNGDIAITAGSTVPENFLLYILAEDSYETPAE
                     GALIDKDTIGTQSAGTVDVNGNISVDGVNVVLAGLYLSLEKIVSAKNAEVTVYGTKKD
                     DSITLKLEGDSSAAVYGGEGNDTITVSGSGGASSDAARGTVTLNGGEGDDLINVDTTL
                     AKNATLANVTGGAGIDRVHLTGTLKQDGTSQAIMIGGVPSITLQDVSEKTIAFLLNST
                     ELYTDALKNKSRVEITQAEIAGGTYTASQPFADYTLAAADLPASTTSVTIQGNGFLAG
                     LIIDGDEYTIHNLNASGLNVTLNGK"
     gene            complement(1803..2420)
                     /locus_tag="PLR57_02400"
     CDS             complement(1803..2420)
                     /locus_tag="PLR57_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40333.1"
                     /translation="MTLKELIAEVVLKIPSKALAGYLSKNIPDWSIMQTATNIIEHCE
                     KVASLALIEQLISVTEEETDRTLLRAALKDLQEFGYIDEETNRIYRERFSEEVFPRYP
                     FLEKCYLPVLFRIGDVISFLNYFKETTYACIEDLPELGDDEDYTGECYYCHDLDCSDP
                     EKVYCDGSAHLHVPLGVADAVDERAISDRQRENLVVFRRCISGQP"
     gene            complement(2464..2988)
                     /locus_tag="PLR57_02405"
     CDS             complement(2464..2988)
                     /locus_tag="PLR57_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40334.1"
                     /translation="MEKYKSWSDLQKLLNDRLFPELRGRVTYFLTRYHDVHNAYGRAA
                     IRVDGKELVCFSWIEQYLQESDLDEALRDHPESNYEQLWETLKPEWDAACQYCEMDFL
                     EAVLQYRSMPVQDALRSENLIIRVLAILDRRTGRRTLERMEVEQELAKSSDWVRQFFD
                     LRLSAMRKQKQTRS"
     gene            complement(3016..>3304)
                     /locus_tag="PLR57_02410"
     CDS             complement(3016..>3304)
                     /locus_tag="PLR57_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HQQ40335.1"
                     /translation="VGPETMSKGRFIFMSLLPSIVFGFVPFLAAMLVPSLVFLGVCGA
                     LSISMGAGDFYNVFNAVTQMPKGARTYLYKFNSFWYLPSENETSKNQVNIH"
BASE COUNT          703 a          880 c          844 g          877 t
ORIGIN      
        1 tgcttgccgt tcagggtgac gttcaggccg gaagcgttga ggttgtgaat cgtgtactcg
       61 tcgccgtcga tgatgagacc ggcgaggaag ccgttacctt gaatggtaac gctggtcgtg
      121 ctggcgggca gatcggccgc cgcaagcgta tagtccgcaa acggctggct cgcggtgtag
      181 gtgccgcccg cgatttccgc ctgcgtgatt tccacgcggc tcttgttctt cagcgcgtcg
      241 gtatagagct cggtcgagtt gaggaggaac gcaatcgtct tttcggaaac gtcctgcagc
      301 gtgatactgg gtacgccgcc gatcatgatc gcctggctcg tgccatcctg cttgagcgtg
      361 ccggtcaggt gaactctgtc gatacccgct ccgccggtaa cgtttgccag cgttgcgttc
      421 tttgccagcg tggtgtcaac gttgatcagg tcgtcgcctt ccccgccgtt cagggttacg
      481 gttccgcggg cggcgtcgct gcttgctccg ccgctgccgg aaacggtgat cgtgtcgttg
      541 ccctcgccgc catacacggc agctgaggag tcgccttcca gcttgagcgt aatcgaatcg
      601 tctttcttgg ttccgtaaac ggtcacttct gcgttctttg cggaaacgat tttttcgagc
      661 gataaataca aacccgccag cacaacgttt actccgtcaa cgctgatgtt tccgttgacg
      721 tcaaccgtgc cggcggactg ggtgccgatg gtgtccttgt cgatgagcgc gccttcggcg
      781 ggcgtttcgt atgaatcttc ggcgaggata tataatagga aattctcggg aacggtgctt
      841 cccgcggtaa tggcaatatc tccgttatag gtgcccgcgg ctacgcggat ggttgcgctc
      901 gtactgtccg gcgcaagttc ggcgagcact ttgtccagtg ctttttgaat cgcgttttcc
      961 gaagcgctga tggtcacggt cgtgcgcgta tcgctcgttt cgttgacctg cgtattgtcc
     1021 ggcgtaaccg tcgtgggggt tgtcagttca accgcttcgc ccgtatcggt cagcgtgttg
     1081 gtaaccgtgg tatcggttac ggttccggtg ccgtcgagcg tttcgatggt ggtggtcgta
     1141 acggagggca aatcgttttc gaatgtcaaa ttgtccggat ccgcgtcgat ttcatccagc
     1201 tggttgccgc tttcgcccga ctgcgtatgt tcgacaatcg tcgtttcgcc ttcggttgct
     1261 gcaaggggca ccgttgcggt aacgtcgccg tcgcccgcgg aagtatcatc ctcaccctcg
     1321 gcaaacgcgc gggaaggaag cccgaaaaaa agagccagca cgacaagccc catcaaaagg
     1381 gaccgcagtg ccagcatcgt ttttttgcgg ttcagtcttc cggccatgga acgaatgagt
     1441 ctcctttgag aaatccagaa tatagcgtcc gatcatgtcg gttattcgtc gttaaacttg
     1501 ttttatcgtc aatccggaga cagccgtttc cgatacactt tacagatcaa tgccattctt
     1561 gtatatacgt tcaatataac tatacacgtc atttttactg gaatccagtg tttttttatt
     1621 ttttgttacc atgaaatatt ttcttgttat tgtttgttga acgataatca ccaaactttg
     1681 aaattctgtt ggttaacaca ttgttcacaa ttcaaaatgc acaatttccc agattacgat
     1741 tagaattcca attagcaaaa aagcggcgaa acgccgcttt tactcgaagc acaatcctca
     1801 ttttacggct gtccgcttat gcagcgccgg aatacaacta ggttctccct ctgccgatcg
     1861 gatatcgcac gctcgtcaac ggcatcggca acgccaagcg gaacatgcag gtgcgcgctt
     1921 ccgtcgcaat agactttctc cggatcagag cagtccaagt cgtggcaata atagcattcg
     1981 cccgtatagt cctcatcatc gcccagctca ggcaagtctt cgatgcaggc gtatgttgtt
     2041 tccttaaagt aattcaaaaa ggaaataacg tctccgatac ggaacagcac aggcaaataa
     2101 catttctcca gaaatggata ccgcgggaaa acttcttcgg aaaaccgttc ccgatagatc
     2161 cggtttgtct cctcatcaat ataaccgaat tcctgcaagt ccttcagagc ggcacgcagt
     2221 aatgttcggt ctgtttcctc ttcggtaacg ctaatcagtt gctcgatcag cgcaagagac
     2281 gccacttttt cgcaatgctc tatgatgttc gttgccgtct gcatgatcga ccaatccggg
     2341 atatttttcg acagataacc ggcaagtgct ttcgacggga ttttaagaac gacttcagca
     2401 atcagttctt tcagcgtcat ggaacgtgct ctctcttcct taattggatt acggtttatt
     2461 gtcttatgag cgagtttgtt tctgcttccg cattgccgac aatctcaagt cgaaaaactg
     2521 gcgcacccaa tcggaactct tcgcaagctc ttgttccact tccatacgct caagcgttcg
     2581 tctgccggtg cgcctgtcca gaatggccag tacacggata atcaaattct ccgaccgcag
     2641 cgcatcctga acgggcatgc tgcggtattg gagcacggct tccaaaaagt ccatttcgca
     2701 atattggcag gccgcgtccc attccggttt cagcgtttcc cacaactgct catagttgct
     2761 ttccggatga tcccgcaaag cttcatcaag gtcgctctcc tgcaggtact gctcaatcca
     2821 ggaaaagcat acgagttctt ttccgtcgac ccgaattgcc gctctgccgt aagcattgtg
     2881 cacgtcgtgg taacgggtca aaaaatatgt cactcttccc cgcagttccg ggaagaggcg
     2941 atcgttcagc agtttttgta agtctgacca ggatttatac ttttccattg cgtcctacca
     3001 tgcagcgtag cctgctcaat gtatgttaac ctggttcttc gacgtttcgt tttccgaagg
     3061 cagataccag aatgaattga atttgtagag atacgttctt gctccctttg gcatctgcgt
     3121 gactgcgttg aatacattgt aaaagtcccc cgctcccatg gaaattgaaa gcgcgccgca
     3181 aacgccgaga aagacgagcg acggaacgag catagcggcg aggaacggaa cgaagccgaa
     3241 cacgatgctg ggcagcagcg acataaagat gaacctgcct ttgctcatcg tttcggggcc
     3301 tacg
//
LOCUS       DAOMGY010000104         3297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30823_length_3297_cov_4.481801, whole genome shotgun sequence.
ACCESSION   DAOMGY010000104 DAOMGY010000000
VERSION     DAOMGY010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30823_length_3297_cov_4.481801"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PLR57_02415"
     CDS             complement(<1..1378)
                     /locus_tag="PLR57_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40336.1"
                     /translation="MKLNLRLSAFALAFCLLLTALPAAALAETETAATATELTEQCVL
                     TPSGDDADKAYRVTDAYTESYLELSKDKPLSITLPAGASAQGLYLEWYDLPDNYTVEQ
                     YDASGTLLSSAERQLYINCYFPLDAAAASIKINCVGDAFLSGLHVYGEGTLPNTVQLW
                     EPTLDQADILFIGATPDAAVTDFYALLALYTVEHQIPTVITVLEKESRTEQGDLLAGL
                     WRMGIRNYPVFGAFSALNNDSYKRVRGWWGVSATHSFIQDQLAAYGEKIVVTHENSEN
                     AFSAAAQYTAEVVLDNTEDADDDYETIQKVYCVSPTGQTAIDLTQELITFDGATLLDA
                     ANIAYQMNADMRRFHKTLAPSAAFTLTYSAVGEDTTAGDLLENIDTATLSGYVSPTPS
                     PTPTPEPTPTPEPTPTATPVAAPVEAVADENESSTIADMEQYMNWGMLIVALIGIIGS
                     LWVVVASFR"
     gene            complement(1476..3113)
                     /locus_tag="PLR57_02420"
     CDS             complement(1476..3113)
                     /locus_tag="PLR57_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HQQ40337.1"
                     /translation="MKRIFLIAVVALAALFMTGCDLFVDRSATELAFDDVQLIDAEDL
                     QKYENLQTLDIRNADVSTELYDQLHAALPNCKILWSVPVGESRIDNQVTQVVLPKIDG
                     EQLTLLSYFPNLTAVDATACDCYGALMTKSLEMQNVAFRWTITIGGVAVQNTDTNADL
                     SGTTTGGMDALGDALSCLPSLMTLNIAETDISGEDAQALMARFPNISFLYTAEIFGVK
                     VNSDATALDLTSAVIDETALVDALAPLKELTTVDLSGQTLSADTMNALRERYPNIQFA
                     FTVDVFGQLVASDANELDLRGNTFTAPEEVAAVLTSLPNLTYCDLCDTGLTNEQMETL
                     MAQFPGVKFVWYVDIGAWRIRTDIEAFSTGNRDEFPNGAGYFTGKGRTSLTDTEVEAL
                     KYCTDLIYLDVGHNKITSLAFVEGLTKLRVLIVGSNKLPDITPVSKLENLEYLEMFMN
                     LVTDISPLSGLTKLQYLNCSRNSFTDITPLLTMTQLKKLWIVNNRQISKDDFTKLIAA
                     LPECEICTSAVHSTANGWRDTDIYKEFQEKFGLPALD"
     gene            complement(3118..>3297)
                     /locus_tag="PLR57_02425"
     CDS             complement(3118..>3297)
                     /locus_tag="PLR57_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40338.1"
                     /translation="SSVMKKANVRTGCTNGWINNTETGTLGADESGSEFNPTDTETGN
                     RNGTNERTIDGKGAE"
BASE COUNT          633 a          954 c          946 g          764 t
ORIGIN      
        1 atcgaaacga tgcgaccact acccacaaac tgccgataat gccgatcaga gcaacgatga
       61 gcattcccca gttcatgtac tgctccatat cggcaatggt cgagctttcg ttttcgtccg
      121 ccacggcttc aacgggagcg gcaaccggcg ttgccgtcgg cgtaggctcc ggcgttggcg
      181 tgggctccgg ggtcggcgtt ggcgacggcg ttggggaaac atacccgctg agcgtcgccg
      241 tgtcgatatt ttcaagcagg tcgcccgcgg tcgtatcctc acccacggcg gaatacgtca
      301 gcgtaaacgc cgcggaaggc gccagcgttt tatggaaacg gcgcatgtcc gcgttcatct
      361 ggtaagcgat gttggcagcg tcgagcaaag tcgcgccgtc aaacgtaatc agctcctgcg
      421 tcaggtcgat tgccgtctga cctgtcggag aaacgcagta taccttttga atggtctcgt
      481 aatcgtcgtc cgcgtcctcg gtattatcca gcacgacttc ggcggtgtac tgcgcagccg
      541 cgctgaacgc attttcgctg ttttcgtgcg tcacgacaat cttttcaccg taggccgcga
      601 gttgatcctg aatgaacgaa tgcgtcgcgc tcacgcccca ccagccgcgc acccgcttgt
      661 aggaatcgtt gttgagcgcg ctgaacgcgc cgaacaccgg atagttcctg attcccatgc
      721 gccagagccc ggcgagcagg tctccctgtt cggtgcggct ctccttttcc agcacggtga
      781 tcacggtggg aatctggtgt tccacggtat agagcgcaag cagcgcgtaa aagtccgtta
      841 ccgcggcgtc aggcgtggct ccaataaaga gaatgtccgc ttggtccagc gtcggctccc
      901 aaagctgcac ggtattaggc agcgtgcctt cgccgtacac atgcaggccg gagaggaacg
      961 cgtcgcctac gcagttgatc ttgattgagg ccgccgccgc gtccagcggg aaatagcagt
     1021 tgatgtaaag ctggcgctcc gcgctggaaa gcagcgttcc ggaagcgtcg tactgttcaa
     1081 cggtatagtt atccggcagg tcgtaccatt ccagatacag cccctgcgcg ctcgcgcccg
     1141 cgggcagcgt gatcgaaagc ggcttgtcct tggaaagctc gagatagctc tccgtatacg
     1201 cgtccgtcac gcggtacgcc ttgtcggcgt cgtctccgga cggcgtaagc acgcattgct
     1261 ccgtcagttc ggttgctgtg gcagcggttt ccgtctccgc gagcgcggcg gcgggaagag
     1321 cggtcagcag caggcaaaac gcgagggcaa acgcggacaa gcgcaggttc agtttcatgg
     1381 tttctctcct ttgggggtgg cggccagccg tcagggttct acgtgcaggc cgggtttcgt
     1441 ggtttcgtgg ttcgttttgg atcggttcgc cgccgctagt cgagcgcggg caggccgaac
     1501 ttctcctgga attccttata gatatccgta tcccgccagc cgttggcggt ggaatgcacg
     1561 gcggacgtgc agatttcgca ctccggcaga gctgcgatga gctttgtaaa atcgtctttg
     1621 gaaatctggc gattgttcac gatccagagt tttttcagct gcgtcattgt caaaagcggc
     1681 gtgatgtccg taaagctgtt gcgactgcag ttgagatact gtaacttcgt cagtccggaa
     1741 agcgggctga tgtcggtcac aaggttcatg aacatctcca gatattcgag gttttcgagc
     1801 ttggaaactg gcgtaatatc cggtaacttg ttgctgccga caatcagaac gcgcagcttc
     1861 gtcaagcctt ctacaaacgc aaggctcgta atcttgttgt gtcctacgtc gaggtagatc
     1921 aaatccgtgc agtacttgag cgcttccact tccgtatccg tcaacgaagt tctgcccttg
     1981 ccggtaaagt accccgcgcc gttggggaac tcgtcgcggt tgccggtgga aaacgcctct
     2041 atatccgtgc gaattctcca ggcgccgata tcgacatacc agacgaactt gacgccgggg
     2101 aactgcgcca tcagcgtttc catctgctcg ttggtcagcc cggtatcgca aaggtcgcaa
     2161 taggttaagt tcggcaggct cgtcagcacg gcagcaactt cttccggcgc agtaaacgtg
     2221 tttccgcgca ggtcgagctc gtttgcgtcg cttgcaacaa gctggccgaa cacgtccaca
     2281 gtaaacgcaa actgaatgtt cgggtagcgc tccctgagcg cgttcatcgt gtccgcgctg
     2341 agcgtctgcc cactgaggtc aaccgtggtc agctccttca gcggcgcgag cgcgtccacg
     2401 agcgctgttt cgtcaataac ggcggaagtc aaatccagcg cagttgcgtc ggagtttacc
     2461 tttacaccaa atatctccgc ggtatagaga aacgaaatgt tcggaaaccg cgccatcagc
     2521 gcctgcgcgt cctctcctga aatatccgtc tccgcaatat tcagcgtcat cagcgaaggc
     2581 agacaggaaa gcgcgtcgcc gagcgcgtcc attcccccgg tcgtcgtgcc ggaaagatcg
     2641 gcgtttgtat cggtattttg aaccgccacg ccgccgatgg tgattgtcca gcgaaacgct
     2701 acgttctgca tttcgaggct ctttgtcatc agcgcgccgt agcagtcgca ggcggtggca
     2761 tccactgccg tcaggttcgg aaaatagctt aaaagcgtga gctgctcgcc gtcgatcttc
     2821 ggcaggacga cctgcgtcac ctggttgtcg atgcggcttt ctcccaccgg cacgctccag
     2881 aggatcttgc agttcggcag cgcggcatgc agctggtcgt acagctccgt agaaacgtcc
     2941 gcgttgcgaa tgtcgagcgt ctgcaggttt tcgtactttt gcaggtcttc cgcgtcgatc
     3001 agttgtacgt cgtcgaaagc cagttccgtt gccgagcgat ccacaaacag atcgcagccc
     3061 gtcatgaaaa gcgcggcaag cgcaaccacc gcgatcagga aaatccgttt catctgctta
     3121 ttccgcccct ttcccgtcaa ttgttcgttc gtttgtccca tttctgtttc ctgtttctgt
     3181 atctgtcggg ttgaattccg agccggattc atcggctccg agcgtcccgg tttctgtgtt
     3241 attaatccag ccgttcgtac agcctgtacg tacgttcgct ttcttcataa cggaact
//
LOCUS       DAOMGY010000105         3295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30846_length_3295_cov_3.078086, whole genome shotgun sequence.
ACCESSION   DAOMGY010000105 DAOMGY010000000
VERSION     DAOMGY010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30846_length_3295_cov_3.078086"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="PLR57_02430"
     CDS             <1..1572
                     /locus_tag="PLR57_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006255895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HQQ40339.1"
                     /translation="KHLPVDFRNGVDVMTTETTCLSSIWETDGEIANYYRTHGRPADF
                     QTLEPEEGAYYDAMITIELDKVECMIALPFHPSNAYPIHEFLERADELLAKVEAEAAE
                     QFPRAKPNLRGKIQPDGVHADQGIIAGCAGGLFDNIVEASEILSGGSIGNGAFSFSVY
                     PTSVPVSLALTKAEATETLIRAGAVIKPSFCGPCFGAGDVPANNGLSIRHTTRNFPNR
                     EGSKPGEGQIATVALMDARSIAATAANGGRITAATDVLYRKTRIKYRYDRSAYEKRCY
                     NGFGKADPSVELVFGPNIADWPKIDKLSDNLLVELSAVIRDPVTTTDELIPSGETSSY
                     RSNPLRLAEFALSRRAPDYVGRSKAVAKAEDARKSGNAPEALKALLGRVGNADELLKT
                     THYGSCVFANKPGDGSAREQAASSQRVLGGAANICYEYATKRYRSNCINWGILPFTLP
                     AGAAFPYEVGDHVYIPGVRAAVESKADTLRAVVLKQDGSKEEITLNLPPLTEDEREIL
                     LEGCLMNYYAAHAGK"
     gene            1643..2854
                     /locus_tag="PLR57_02435"
     CDS             1643..2854
                     /locus_tag="PLR57_02435"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQQ40340.1"
                     /translation="MSLISMKTPLVEMDGDEMTRVLWQQIKEELILPFVDLKVEYYDL
                     GLPEREKTRDQVTTDSANAIKKYGVGVKCATITPNAQRVKEYNLTEMWKSPNGTIRAI
                     LDGTVFRAPILIDSIKPVVKNWKKPITIARHAYGDVYRGTEYRVPEKGKAELIFTGEN
                     GASFRKTVYDFECPGVLQAMYNKDSSIKSFAHSCFRYALDTKQDLWFSTKDTISKLYD
                     HTFKDIFEEIFESDYKAAFEEKGLAYFYTLIDDAVARVIRSEGGFIWACKNYDGDVMS
                     DMVSTAFGSLAMMTSVLVSPDGNYEFEAAHGTVTKHYYRYLEGGETSTNPMAMIFAWS
                     GAFKKRGELDGLPQLTAFGEAMEAASIETLDAGVMTKDLVGLCEGTQPKAVTSIAFLR
                     VVREHLEAKMA"
     gene            3058..>3295
                     /locus_tag="PLR57_02440"
     CDS             3058..>3295
                     /locus_tag="PLR57_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40341.1"
                     /translation="MKHEIFKRIGAILTAAVLIFVFGCTKSAPVKQSVETEQPQTQTE
                     TSEFTGYSGVYHADGTNETVENASYATSTANENAL"
BASE COUNT          777 a         1022 c          925 g          571 t
ORIGIN      
        1 aaacacctgc cggtcgattt ccgcaacggc gtggacgtga tgaccaccga aaccacctgc
       61 ctttcctcta tttgggaaac ggacggcgaa attgcgaatt actaccgcac gcacggccgc
      121 cccgcggact tccagacgct ggagccggag gaaggcgcgt attacgacgc catgatcacc
      181 atcgagctcg acaaggtcga gtgcatgatc gcgctgccgt tccaccccag caacgcctac
      241 cccatccacg agttcctgga gcgggcggac gagctgctcg ccaaggtgga agcggaagcg
      301 gctgagcagt tcccgcgcgc aaaaccgaac ctgcgcggaa aaatccagcc ggacggcgtg
      361 cacgccgatc agggcatcat cgcgggctgc gcgggcggtc tgttcgacaa catcgtggaa
      421 gcttccgaaa tcctctccgg cggctccatc ggaaacggcg cgttctcgtt ctccgtttac
      481 cccaccagcg tgccggtcag cctcgcgctg accaaggcgg aagcgactga aacgctcatc
      541 cgcgcgggcg ccgtcatcaa accctcgttc tgcgggccgt gcttcggcgc gggcgacgtc
      601 cccgccaaca acggcctgtc gattcggcat accacgcgca acttccccaa ccgcgaaggc
      661 agtaagcccg gcgaagggca aatcgccacc gtcgcgctca tggacgcgcg ttcaatcgcc
      721 gcaaccgccg ccaacggcgg acgcatcacg gcggccaccg acgtgctcta ccgcaagacg
      781 cgcatcaaat accgttacga ccgttctgct tatgaaaagc gctgctacaa cggttttggc
      841 aaggcggatc cttccgtcga gctcgtattc gggccgaaca ttgcggactg gccgaagatc
      901 gacaaactga gcgacaacct gctcgtcgag ctctcggcgg tcatccgcga ccccgtcacc
      961 acgacggacg agctgattcc ctccggcgaa acaagttcct atcgttctaa ccccctgcgg
     1021 ttggcggagt ttgcgctctc gcgccgcgcg ccggactatg taggccgcag caaggcggtc
     1081 gccaaagcgg aagacgcgcg taagagcggc aacgcgccgg aagcgctgaa agccctgctc
     1141 gggcgggtcg gaaacgcgga cgagctcctc aagaccacgc actacggctc ctgcgtgttc
     1201 gccaacaaac ccggcgacgg cagcgcgcgc gaacaggccg cgtccagcca gcgcgtactc
     1261 gggggagccg cgaacatctg ctacgaatac gccaccaagc gctaccgcag caactgcatc
     1321 aactggggca tcctgccgtt tacgctcccc gcgggcgcgg cgttccccta tgaagtgggc
     1381 gaccatgtgt atatccccgg cgtgcgcgcc gcggtcgaaa gcaaggccga tacgctgcgc
     1441 gctgtcgtgc tcaaacagga cggttcgaag gaggaaatca ccctcaacct gccgccgctg
     1501 acggaggacg agcgcgaaat cctgctcgaa ggatgtctga tgaactacta cgccgctcac
     1561 gcgggaaaat agttgccaat gctttcgcta gaagtttttg cttcacgcga acgataaaac
     1621 ggatttttga aaggaaacga ctatgagcct gatttccatg aagaccccgc tcgtcgagat
     1681 ggacggcgac gaaatgaccc gcgtcctctg gcagcagatt aaggaagaac tgattctgcc
     1741 gttcgtcgat ctcaaggtgg aatattacga cctcggcctg ccggaacgcg aaaagacacg
     1801 cgaccaggtg actacggaca gcgcgaacgc catcaaaaaa tacggcgtgg gcgttaagtg
     1861 cgcaaccatc accccgaacg cccagcgcgt gaaggaatat aacctcaccg agatgtggaa
     1921 gagccccaac ggcaccatcc gcgccatatt ggacggaacg gtgttccgcg cgccgatttt
     1981 gatcgactcg atcaagcccg tcgtcaaaaa ctggaaaaag cccatcacaa tcgcccgtca
     2041 cgcctatggc gacgtgtacc gcggcacaga ataccgcgtg ccggaaaagg gcaaggccga
     2101 gctgatcttt accggcgaaa acggcgcgag cttccgcaag acggtttacg actttgagtg
     2161 ccccggcgtt ctgcaggcca tgtacaacaa ggacagctcg atcaagtcct ttgcgcacag
     2221 ctgcttccgc tatgcgctgg atacaaagca ggacctgtgg ttttccacca aggacaccat
     2281 cagcaagctc tacgaccata cgtttaagga tatcttcgaa gagatctttg aaagcgacta
     2341 caaagccgcg ttcgaagaaa aggggcttgc ctatttctat acgcttatcg acgacgcggt
     2401 ggcgcgcgtc atccgctccg aaggcggatt catctgggcc tgcaaaaact acgacggcga
     2461 cgtgatgagc gacatggtca gcacggcgtt cggcagcctt gccatgatga cgagcgttct
     2521 cgtttctccg gacggaaatt acgagttcga agccgcacac ggcaccgtaa ccaaacacta
     2581 ttaccgctac ctcgaaggcg gcgaaaccag cacaaacccc atggcgatga tctttgcctg
     2641 gagcggcgcg ttcaaaaagc gcggcgagct ggacggtctg ccccaactga ccgcctttgg
     2701 cgaagcgatg gaagcggcga gcattgaaac gctggatgcg ggcgtgatga ccaaggacct
     2761 cgtaggcctt tgcgaaggca cgcagcccaa ggcggttacg tccattgcgt tcctccgcgt
     2821 ggtgcgcgag catctggaag ccaaaatggc ctgatcgacc gaatgaaacg gcatcctgcg
     2881 cccgcgcgat tgttttgcgc gggcgctttt tcattagttt cctttcctga aatgcaaacg
     2941 catggatacg aagtttgaca attcattcaa acaccgtcca cgccaatgac acaactgcac
     3001 gataagataa attgatatat tgtatccccc atcctttgat tgaaggagca tataaatatg
     3061 aagcatgaaa tctttaagcg catcggcgca attctgacgg cggccgtgct gattttcgta
     3121 ttcggctgca caaagagcgc accggttaag cagtctgttg aaacggaaca gccgcaaacg
     3181 caaacggaaa ccagcgagtt caccggctac agcggcgtgt accatgcgga cggcacaaac
     3241 gagaccgtcg aaaacgcatc ctacgccaca tccaccgcaa acgaaaacgc gctgc
//
LOCUS       DAOMGY010000106         3287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30955_length_3287_cov_2.983292, whole genome shotgun sequence.
ACCESSION   DAOMGY010000106 DAOMGY010000000
VERSION     DAOMGY010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30955_length_3287_cov_2.983292"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="PLR57_02445"
     CDS             <1..1572
                     /locus_tag="PLR57_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40342.1"
                     /translation="TKIATIAPEAKAIRVYDRSDGSSTDFDYDVRQEGATLLEYAPDG
                     ALYLCDSTGLYRHTQDGTLWERIMDGSTCNLGLPSFLPIGMTTAQGQPDAIYVYGEGS
                     TLLKYWFDPAAPMAASKTISVFSLHADETVQQAIVVFNRSQSEFAASYTAAMEQGAAG
                     TEQDYIKALNTELLAGTGPDVLILDGLPVNSYIQKGVLADIGAIVDDAEPVLSNIRAA
                     SMAGDGVLYAMPSGIRLPFAYAPGGAESAFASLSALANACENAGEVPLLASAAFNYQT
                     LAEVLLNYYGGSLLTGNTDDIRSFLTDTGRVARSIGTDDKLCEGWEAVQGTPDEELLT
                     AMRINNGGPQIWADMTGRAQGALLLPSGSFYSEMIVFSAAEALNAALTGIANQYEPVG
                     LVGVNRASASLDAAAQFVQTLLSFQVQSANKYSDAFPVNVEALNNKAANVDDTMSQSM
                     RFDETNSLSAEWPSEAVRVQFHALAQQVNSPLMTDTVFGDMVTPAIVSYLDGSDTLET
                     AVVKLESVISTYLSE"
     gene            1581..2441
                     /locus_tag="PLR57_02450"
     CDS             1581..2441
                     /locus_tag="PLR57_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQQ40343.1"
                     /translation="MKPKVGGEGRAALAFLAPSLIGIFGMVLLPTADVVRRSFLSAMG
                     NSFVGLENYRTILLNASFRLAVANTARFLITCIPLLLLSSLTVALMLYSRTRLQNLFR
                     TTYLLPLVVPVTSVVMIIQTVFHKNGLLSTLCAAFGLAPHDWLNSEYAFFVMVLCYLW
                     KYFGYNVILWMAGLAAIPESYYEAARVDGANEAQCFFHITLPQLGGTLFMTSVLALIN
                     SFKVFREAYLVAGRYPNSSIYMLQHVLSNWFLNLDMQKISAAAVLLMCTILLVVFVTQ
                     RALDRKREVL"
     gene            2438..>3287
                     /locus_tag="PLR57_02455"
     CDS             2438..>3287
                     /locus_tag="PLR57_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQQ40344.1"
                     /translation="MKTIRLRPVSFVLLFLLALLVWMPLWMLVTGALTPTDELRTHLP
                     GVLGASAELASWNLLPNRVTLRHVVELLLDTPAFFRVFWNSCVQVFPAVLGQILVGAP
                     AAWALSQMCFRGRNALFRLYIVLMILPFQVTMVSSYIVLDRLGLVDTPFAVLLPAAFS
                     AFPVFIMTKFFAGIPREMLEAARMDGAGEWSIFFRIGLPLGLPGVLSAALLGFVEGWN
                     AVEQPITFLKTPSMYPLSIFLPATTVENAGVSFVSAVITLLPAVLIFLFGQQYLEQGI
                     IASGIKE"
BASE COUNT          626 a          895 c          985 g          781 t
ORIGIN      
        1 accaaaattg cgacaatcgc accggaagca aaagcaatcc gggtgtacga ccgttcggac
       61 gggtccagca cggacttcga ttacgatgtt cgtcaggagg gcgcgactct tttggaatat
      121 gcgcccgatg gagcgctgta tctttgcgac agcacggggc tctatcgcca tacgcaagac
      181 ggaacgctct gggaacgcat tatggacgga agcacctgca acctcggttt accttccttt
      241 ctgccgattg gaatgacaac ggcgcagggc cagccggatg cgatctatgt ctacggtgaa
      301 ggatcgacgc tgctgaagta ttggttcgat ccggcggctc cgatggccgc ttcgaaaacg
      361 atcagcgtgt tctccctgca cgcggacgaa acggttcaac aggctatcgt cgtgttcaac
      421 cgttcgcaga gcgaatttgc cgccagctat accgccgcaa tggaacaggg cgcggccggg
      481 acggaacagg attacatcaa ggcgctcaac acggaacttc tcgcgggcac ggggccggat
      541 gtgctgattc tcgacgggct tccggtgaac tcgtatattc agaaaggcgt tcttgcagat
      601 atcggtgcta tcgtggacga cgctgagccg gtgctgagca atatccgcgc ggcctccatg
      661 gcaggcgacg gtgttttgta cgccatgccg agtggaatcc gtttgccgtt tgcatatgcg
      721 ccgggcggcg cggaaagtgc gtttgcatcg ttgtccgctc tggcgaacgc ctgtgaaaac
      781 gcgggagaag tacccctgct cgccagtgcc gcgttcaact atcagacgct tgcggaagtt
      841 ctgctgaact actacggagg aagcctgctc acaggcaata ccgatgatat ccgctcgttt
      901 ttaacggaca ccgggcgtgt tgcccggagc atcggtaccg acgataagct ctgcgaaggt
      961 tgggaagccg tgcagggcac gcctgacgaa gagctcctga cagccatgcg catcaataac
     1021 ggcggcccgc agatctgggc ggacatgacc ggcagggcgc agggggcgct gttgctgccg
     1081 tccggttcgt tctacagcga gatgatcgtt tttagcgcgg cggaggcgct gaacgccgcc
     1141 ctcacgggaa tagccaacca gtacgaaccg gtggggctgg tcggtgtcaa ccgcgcgagc
     1201 gcaagtttgg acgcggcggc gcagtttgtg cagaccctgc tctctttcca ggtgcagagc
     1261 gccaacaagt attccgacgc gttccctgtg aacgtggaag cgctgaacaa taaagccgca
     1321 aacgtggacg atacgatgtc ccagtccatg cggttcgatg aaacgaacag cctgagcgca
     1381 gaatggcctt ccgaagcggt tcgggtgcag ttccatgcgc tggctcagca ggtaaacagc
     1441 ccgctgatga cggataccgt gttcggcgac atggtcacac cggccatcgt ttcttacctt
     1501 gacgggagcg atacgctgga aaccgccgtg gtcaagctgg aaagcgtcat ttccacatat
     1561 ttgtccgaat gaaacgggcg ttgaaaccga aagtgggcgg ggaagggcga gctgcgctcg
     1621 cctttctcgc ccccagcctg atcggcattt tcggcatggt gcttttgccg acggcagatg
     1681 ttgtgagaag gtcgtttctc tctgcaatgg gcaactcgtt tgtcgggctg gaaaattacc
     1741 ggacaattct gctcaacgcg tcctttcggc tcgcggtggc caacacggct cggttcctga
     1801 ttacctgcat tccactactg ctgctgagtt cgctcaccgt ggctctgatg ctctattcga
     1861 gaacgcgcct gcagaatttg ttccgcacca cgtatctgct gccgctggtg gtgccggtga
     1921 cgagcgtcgt gatgattatt caaaccgttt ttcataagaa cgggctgctt tcgacgctct
     1981 gcgccgcgtt cggcctggct ccgcatgact ggctcaacag cgagtacgcc ttttttgtga
     2041 tggtgctctg ttacctttgg aagtacttcg gctataacgt gatcctttgg atggcggggc
     2101 tggcggcgat tccggagtct tattacgaag cggcgcgcgt ggacggcgca aacgaagcgc
     2161 agtgcttttt tcatatcacc ctgccgcagc tcggcggaac gttgtttatg accagcgtgc
     2221 tcgcgctgat caactccttt aaggtgtttc gtgaagcgta tctggtcgca ggacgctatc
     2281 ccaacagcag tatttacatg ctccagcacg tgctttccaa ctggtttttg aatttggata
     2341 tgcaaaagat cagcgcggct gcggtgctgc tcatgtgcac aattctgctt gttgtgttcg
     2401 ttacgcaaag agcgctggat cgaaagaggg aggtcctatg aaaacaatac ggctgcgtcc
     2461 ggtttcgttt gtgctgctct ttcttctggc tttgctggtc tggatgccgc tttggatgct
     2521 cgtcaccggc gcactgacgc ccacggacga actaagaacg catcttcccg gcgtgctcgg
     2581 tgcgtccgcc gaacttgcaa gttggaacct gcttcccaac cgcgtgacgc tgcggcatgt
     2641 cgttgaactg ctgctggaca cgcccgcgtt ctttcgggtg ttctggaact cctgcgttca
     2701 ggtatttccc gcggtattgg ggcagatact ggttggcgcg cctgcggcct gggcgctgtc
     2761 ccaaatgtgt tttcgcggca gaaacgcgct atttcgcctc tatatcgtgc tcatgatttt
     2821 gccgtttcaa gtgacgatgg tctccagcta tatcgtgctg gaccggctcg ggctggtcga
     2881 tacgccgttt gcggtactgc tgccagccgc gttttctgcg tttccggtgt ttattatgac
     2941 aaaattcttt gcgggaatcc ctcgggaaat gctggaggcg gcgaggatgg atggcgcggg
     3001 cgaatggtcc atcttctttc gaatcggact gccgcttggc ctgccgggcg ttctctccgc
     3061 ggcgcttctc ggttttgtgg aaggctggaa cgccgtagaa cagccgatta cgtttctcaa
     3121 aacaccatca atgtatccgc tttccatttt tctgcctgca acgacggtgg aaaatgcagg
     3181 ggtttcgttt gtttccgcgg tgattacgct gctgcccgcg gtgctgatct tcttattcgg
     3241 gcagcaatat ctcgaacagg gcatcattgc ctcgggaatc aaggagt
//
LOCUS       DAOMGY010000107         3286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_30968_length_3286_cov_3.201795, whole genome shotgun sequence.
ACCESSION   DAOMGY010000107 DAOMGY010000000
VERSION     DAOMGY010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30968_length_3286_cov_3.201795"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLR57_02460"
     CDS             <1..563
                     /locus_tag="PLR57_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40345.1"
                     /translation="TDTDYTDFFGTYYLDEGTNPGNAAFALFIVGAAFSGLILLILIA
                     VSASIQNRYKKSDERLYAFGKSDDAEFEFTAMDNLRFEKSKLILSEHFAYAGSTGVVA
                     SYEDIIWLYKYVQRNHGVVVSTQLIAGLINGRTAALATRFVTDELIEQAVMKILRQNP
                     NVLVGYSIDNARQFRARVKEFKLNNR"
     gene            841..1407
                     /locus_tag="PLR57_02465"
     CDS             841..1407
                     /locus_tag="PLR57_02465"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HQQ40346.1"
                     /translation="MDRRVRKTRMQLRAGLTQLMRQKPIKDITVRELAQLVDINRCTF
                     YLHYRDIYDMVEQVEQEVFSEFEALVSAHPPKEIQNKPMPMLLDLYTFFSDNADLCAA
                     FLGGNGDITFVNKIIGLIRDRVLEYWLQERKFSPEQFDYYFSFMASGCIGMIREWFRR
                     DMLETPTEMAELTEKMILCGASAVNDKE"
     gene            1510..>3286
                     /gene="ftsH"
                     /locus_tag="PLR57_02470"
     CDS             1510..>3286
                     /gene="ftsH"
                     /locus_tag="PLR57_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQQ40347.1"
                     /translation="MKEHTPSKRPIIYYAVVAAIMLLLLNALVFPGLLQAEVKEVGYN
                     EFLKMVESGTVTEVARDEAAGTITFTTGKDSTVQYYKTGIWPDDTLVDRLNQAGVQFS
                     ASIPTQASPLISFLVSWVLPILFFVWIGRFMMKRMQSGPGGLGNALSFGKANAKIYVE
                     AQTGKTFEDVAGQDEAKEALLEIVDFLHNPNRYADIGAKLPKGALLVGPPGTGKTLLA
                     KAVAGEANVPFFSISGSEFVEMFVGMGAAKVRDLFSQAEQKAPCIVFIDEIDTIGKRR
                     DNGLGSNDEREQTLNQLLTEMDGFDARKGVVILAATNRPDSLDPALLRPGRFDRRIHV
                     ELPDLKGRTDILRVHARGVKIDPNVDFEAVARATSGASGADLANIINEAALRAVRLKR
                     EIVEQSDLEESVEVVIAGYARKNAVISPKEKRIVAYHEIGHALVAAKQTDSAPVHKIT
                     IVPRTSGALGYTMQVDESEHYLMTKEEAENKIATLTGGRSAEEIVFGSVTTGASNDIE
                     QATRIARAMVVRYGMSGTFGMVALECQSNPYLGGDNSLTCSSDTAAKVDAEIVTIVEA
                     AHQRALKILRENEGALHTLAEYLLER"
BASE COUNT          759 a          950 c          903 g          674 t
ORIGIN      
        1 taacggatac ggattatacg gacttctttg gcacttacta tctcgacgaa ggcacaaatc
       61 ccggcaatgc cgcgtttgcg ctgtttatcg taggcgcggc gttttccggt ttgattttgc
      121 tcattcttat tgccgtttcc gcgagcattc aaaaccgtta caaaaagagc gacgagcgtc
      181 tttatgcgtt cggcaaatcc gacgacgcgg agtttgaatt taccgccatg gacaatctgc
      241 ggttcgaaaa atcaaagctc atcctctccg agcactttgc gtatgcgggt tccacaggcg
      301 ttgtcgcgtc ttacgaagat atcatatggc tctacaaata tgttcagcgc aaccacggcg
      361 tcgtcgtctc cacacagctc atcgcggggc tgatcaacgg tcgcaccgcc gcgctggcca
      421 cccggtttgt caccgacgag ctcattgaac aggccgtcat gaaaatactc cggcaaaacc
      481 cgaacgtgct ggttggctac tccattgata acgcccggca gttccgcgcc agagtaaaag
      541 aattcaagct gaacaatcgc tgaaagtaag aaaaaaggga cgcgtttgcg tcctttttta
      601 tttttcctca tatgaacaag agatagaacc aaaagttgaa ttttgtcttt ttcgcgacgc
      661 caagttcacg ttgggttaac accagagtca cttcgttctg ttgatttatc atccggaata
      721 gagtatatta aacccagttg tagcaacatc atacaacagt gtttccgccg ttctccggcg
      781 gctctcaaat aatcaactat aaacagaatc agggggcgtt gcattttgga aacgaaaaaa
      841 atggaccgac gcgtcagaaa aacgcgaatg cagctgcgcg cggggctgac ccagctcatg
      901 cgtcaaaagc cgatcaagga catcaccgtc cgcgagctgg cgcagcttgt ggacatcaac
      961 cgctgcacgt tttacctgca ctatcgggat atttacgata tggtcgagca ggtggagcag
     1021 gaagtctttt ccgagttcga agctctggtt tccgcgcatc cgcccaagga aattcagaac
     1081 aagcccatgc ccatgctgct ggatctgtac acctttttct ccgacaatgc ggatctctgc
     1141 gcggcgtttt taggcggaaa cggggacatt acgttcgtca acaagatcat cggcctgatt
     1201 cgcgaccgcg tgctggagta ctggctgcag gaacgaaagt tcagccccga gcagtttgac
     1261 tactactttt cgttcatggc atccggctgc atcggtatga tccgcgaatg gttccgccgg
     1321 gatatgctcg aaacgccgac cgagatggcc gaactcaccg aaaagatgat tctctgcggc
     1381 gcttccgccg ttaacgacaa ggaatagcat tcacactgcg actaagtatt acaccatcta
     1441 atatattctc tgctgccgcc gcacccaaaa cagcaaaccg cggcgggtac ccgaaaggac
     1501 aaacgctcta tgaaagaaca tacgccttcc aaacggccga tcatctacta tgccgtagtt
     1561 gcggccatca tgctgcttct gctcaacgcg ctggtgtttc cggggctttt gcaggccgaa
     1621 gtgaaggaag ttggatacaa cgaattcctc aaaatggtgg aaagcggaac ggtaacggag
     1681 gtcgcgcggg acgaagccgc gggcaccatc acctttacca ccggcaagga cagcacggtt
     1741 caatattaca aaacaggcat ctggccggac gatacgctgg tcgaccgtct caatcaggcg
     1801 ggcgtgcagt tctccgccag cattcccacg caggcgtccc cgctgatcag ctttctggtg
     1861 agctgggtgc tgccgattct gtttttcgtg tggatcggcc gctttatgat gaagcgcatg
     1921 cagagcggcc ccggtggcct cggcaacgcg ctgtcatttg gcaaggccaa cgcgaaaatc
     1981 tatgtggaag cgcagaccgg caagacattt gaagacgttg ccgggcagga cgaagccaag
     2041 gaagcgctgc ttgaaatcgt ggactttctc cataacccga accgctatgc ggatatcggc
     2101 gcaaagctcc ccaaaggcgc gctgctcgta ggccctccgg gcacgggtaa aacgctcctg
     2161 gcaaaagccg tcgccggcga agcgaacgtg ccgtttttca gcatctctgg ctcggaattt
     2221 gtggaaatgt tcgtgggcat gggcgcggcc aaggtgcgcg acctgttttc gcaggccgag
     2281 cagaaagctc cctgcatcgt atttatcgac gaaatcgaca ccatcggcaa acgccgtgac
     2341 aacggcctcg gcagcaacga cgagcgcgag cagaccctca accagctcct caccgagatg
     2401 gatggcttcg acgcgcgcaa gggcgttgtc atcctcgccg ccaccaaccg tccggactcg
     2461 ctcgaccccg cgctgctgcg tcctggccgc ttcgaccgcc gcatccacgt ggaactgccc
     2521 gacctcaagg ggcgcacgga cattctgcgc gtccacgcgc gtggcgtgaa gatcgatccc
     2581 aatgtggact ttgaggccgt cgcccgcgcc accagcggcg catccggcgc cgatcttgcc
     2641 aatatcatca acgaagccgc gctccgcgcc gtccgcctga aacgggaaat cgttgagcag
     2701 agcgatctgg aagagagcgt ggaagttgtc attgcgggtt acgcgcgcaa aaacgccgtc
     2761 atctctccca aggagaagcg gattgtggct tatcacgaga tcggccacgc gctcgttgcc
     2821 gcaaagcaga cggactccgc gcccgtgcac aagattacca tcgttccgcg cacatcgggc
     2881 gcgctgggct acaccatgca ggtggacgag tccgagcact acctgatgac caaggaggaa
     2941 gccgagaata agatcgcaac ccttaccggc ggacgttccg cggaggagat tgtgttcggt
     3001 tccgtcacta ccggcgccag caacgatatt gagcaggcga cgcgcatcgc ccgcgcaatg
     3061 gtcgtgcgct acggcatgag cggcacgttc ggcatggtgg ctctggagtg ccagtccaac
     3121 ccctacctcg gcggggacaa ttcgctcacc tgctcttcgg acaccgccgc caaggtggac
     3181 gcggaaatcg tcactatcgt ggaagccgcg catcagcgcg cactgaagat cctgcgggaa
     3241 aacgaaggcg cgctgcatac gctggcggaa tatctgctgg agcgcg
//
LOCUS       DAOMGY010000108         3280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_31041_length_3280_cov_3.533023, whole genome shotgun sequence.
ACCESSION   DAOMGY010000108 DAOMGY010000000
VERSION     DAOMGY010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31041_length_3280_cov_3.533023"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1460)
                     /locus_tag="PLR57_02475"
     CDS             complement(96..1460)
                     /locus_tag="PLR57_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HQQ40348.1"
                     /translation="MKKFHFTKAAAALMTLALALVIVGLPGIAKADITAISVTPTAGQ
                     STDIAVGGTVQLNATYTGTTTGVVTYTWGATGGGANVSFGSASASGDQITVTGLQAAS
                     GIVVTATLVIDGAPTGITNSITINVLPMTISETSKALVGGDSFTLSVQRTAAGTLTWS
                     TSDATVATVAGGLVSAVGAGTATITATYTYLSNTQEKTCTVTVSPIITLTPASANITT
                     AGGSQTFTLTVLYGGSIYASGSAVEWTNSNGAAGSITASSTALSGSPLAATATFTAPT
                     TMTNATTTLTALVRGTGSNTTTRTATINVRNTRYLTLTGDANLSNSDRYGNYIVTLHE
                     ADGSVVNDDTSTVHWSWSSSYLKISSAALNDSRADMEDGTAEIQLYARYNTPTAGTKL
                     YAWINDDSAHKVSYTIYITGLSSLPQTGQDFTLVYVFGGLCAALLVAAGVWYGIRKKR
                     VTNA"
     gene            complement(1672..>3280)
                     /locus_tag="PLR57_02480"
     CDS             complement(1672..>3280)
                     /locus_tag="PLR57_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HQQ40349.1"
                     /translation="PLAANIAGETFTLNYVPVREQETKSAQRPTLRRALRASNGEVTL
                     LRYDSARGNRVYERTMLFVFLLAMRKRYPKTRVRINYAVGAGLYTNVGDNMCLSPGEV
                     EGIKQEMRAIVNADLTLERKRVDIDDAIDFFSADGQEDKVRLLQWRQFSYFDVYRQED
                     YVDYFYGEMAPSTGYASVFDLQALPTGLFLIRPSSENPNKPAKHVFLPHLTSVFSESE
                     EWGRLMHCAVVADLNDMVLSGEIRNLIRVNEALHEKRFAALADEIIGRGARAILIAGP
                     SSSGKTTSANRLCTQLRVHGKSPVLVSLDDYYLDREKILPGPDGKIDLEHFNTIDVEQ
                     FQQDLTRLLRGEEVELPKFDFIRQHRCEKGVLLRLDETTPLVIEGLHGLNPHLLPDAI
                     DKKLIFRLYVSALTTLNLDDHNRVPTTSIRLLRRMVRDNETRGASVERTLSMWESVRA
                     GEEKWIFPFQEDADAIFNTTLIYEPAVLKKHIFPLLTAVKPESPYYDEAHSIVKFLNY
                     FVEANVEDEIPPTSILREFIGGNTFYR"
BASE COUNT          640 a          901 c          987 g          752 t
ORIGIN      
        1 gcgcgcggat ggcgcgcatc tcttgctgcg ctttggaacg gtgtttaggt ttcttaccgc
       61 ttggggtaac ggtcagttcg gtatgttctt cgcgcttacg cgttggtgac gcgttttttc
      121 cgaatgccat accaaacccc cgccgcgacg agaagcgccg cgcaaagacc gccaaacaca
      181 tacacgagcg tgaaatcctg accggtctgc ggcaggctgc tcagtcccgt gatgtagatg
      241 gtataggaga ccttgtgcgc gctgtcatcg ttgatccagg cgtagagctt ggtgccggcg
      301 gtcggggtgt tgtaacgcgc gtagagctga atctccgcgg tgccgtcttc catatcggcg
      361 cggctgtcgt tgagcgctgc gctcgaaatt ttgaggtagg aagaagacca gctccagtgc
      421 acggtgctcg tgtcgtcgtt cacgacagac ccgtcggctt cgtgcagggt tacgatatag
      481 ttgccgtaac ggtcggagtt gctcaggttc gcatcaccgg tcagggtgag ataacgtgtg
      541 ttgcgaacgt tgatggttgc ggttctagtc gtcgtgttgg atcccgtgcc gcgtaccagt
      601 gcggtcagcg tggttgtcgc attggtcatc gtggtgggag cggtgaaagt cgccgttgcc
      661 gccagcgggc tgccggagag cgcggttgag cttgcggtaa tgctgcccgc agctccgttg
      721 gagttcgtcc attccacagc agagccggag gcgtaaatcg agccgccata caagacggtc
      781 agcgtgaatg tctgcgaacc gcccgccgtg gtaatgttgg ccgacgccgg agtcagcgtg
      841 ataatcggcg aaacggtcac cgtgcaggtt ttctcctgcg tgttggagag gtaagtatac
      901 gtagccgtga tagtcgccgt gcctgcgcca actgcgctga cgagaccgcc ggcaaccgtt
      961 gccaccgtcg catccgatgt agaccatgtg agcgttcctg cggctgtccg ctgtacgctc
     1021 agcgtgaacg aatcgccgcc gacgagcgct ttgctcgttt cggaaattgt catgggcaac
     1081 acgttgatcg tgatggaatt cgttatccct gtcggcgcgc cgtcaatcac gagcgtggct
     1141 gtgacaacaa taccgctggc ggcctgcaga ccggtaacgg tgatctgatc gccgcttgca
     1201 gaggctgagc cgaaagaaac gtttgctcca ccgcctgtag caccccaggt atacgttacg
     1261 acaccggttg tggtaccggt atacgtcgcg ttcagctgga cagtgccgcc gacggcaata
     1321 tccgtgctct gacccgccgt cggggtgacg ctgatggcgg taatgtccgc tttggcgata
     1381 cccggcagac cgacgatgac aagcgcgagc gccagggtca tcagtgctgc cgcggctttt
     1441 gtgaaatgga attttttcat tctgaagttc ctccttttaa tacgaacgga acgctggatt
     1501 tcggattttt acgcaatgga ttgataccat aatacgaatc gcttcctata ttgtcaacaa
     1561 aagtgcataa tcgttttcgg tttcttaacc gaaacgttac gaatacgcca agttgtatcg
     1621 acattttggc tttgtggctg aaaaaagaaa gccacaaaac gcgcaattcg gttatcgata
     1681 aaacgtattt ccgccgataa actctcgtaa aatcgacgtc ggcggaattt cgtcctccac
     1741 attcgcttct acgaagtagt tcaggaattt aacgatggaa tgcgcttcgt cataatacgg
     1801 actttcgggc tttacggcgg tcagcaacgg gaaaatatgc ttcttgagca ccgcgggctc
     1861 gtagatgagc gtcgtgttga aaatggcgtc cgcatcctcc tggaacggga aaatccattt
     1921 ttcctccccc gcgcggacgc tctcccacat ggaaagcgtg cgttcaacgg aagcgcctcg
     1981 cgtttcgttg tcccgcacca tgcgccgcag cagccgaata gacgttgtgg gcacgcggtt
     2041 gtggtcgtcg aggttcaatg tggtcagcgc ggaaacgtag agccggaaga tcagcttttt
     2101 gtcgatcgca tccggcagca gatgggggtt gaggccgtgc agaccctcga ttaccagcgg
     2161 cgtcgtttcg tccagccgca gcagaacgcc tttttcacag cggtgctggc ggataaagtc
     2221 gaacttcggc agttctactt cttcaccgcg caacagccgg gtcaaatcct gctgaaactg
     2281 ctctacgtcg attgtgttaa agtgctccag atcgattttt ccgtcaggtc cgggcagtat
     2341 tttttcccgg tcgaggtagt agtcgtccag agaaacgagt acgggggact tgccgtggac
     2401 gcgcagctgc gtgcagaggc ggtttgcgct cgtggttttg ccggaactcg aaggcccggc
     2461 aatcagtatg gcgcgcgcgc cgcgcccgat aatttcgtcc gccagcgccg caaagcgctt
     2521 ttcgtgcagc gcttcgttga cgcggatcag gttgcggatt tcgccgctga gcaccatgtc
     2581 gttgaggtct gcaactaccg cgcagtgcat gagccgtccc cactcctcgc tttcggagaa
     2641 cacgctcgtt aaatgcggca aaaacacgtg cttggcgggt ttgtttggat tttcggaaga
     2701 cgggcgaatc aagaacagcc ccgtcggcag cgcctgcagg tcaaacacgc tggcgtagcc
     2761 ggtggaaggg gccatttcgc cgtagaagta gtccacatag tcttcctgac ggtaaacgtc
     2821 gaaatacgaa aactgccgcc actgcagaag ccgaactttg tcctcctgcc cgtcagcgct
     2881 gaaaaagtcg atggcgtcgt caatatccac gcgtttgcgc tccagcgtca gatccgcgtt
     2941 tacaatcgcg cgcatctcct gcttgattcc ttccacctcg ccgggcgaaa ggcacatgtt
     3001 gtcgccaacg tttgtgtaga gccccgcgcc gacggcgtag ttgatgcgaa cccgcgtctt
     3061 ggggtagcgc ttgcgcatgg cgagcagaaa cacaaacagc atagtgcgct catacacgcg
     3121 gttgccgcgc gcgctgtcgt acctgagcag ggtgacttcg ccgttgcttg cgcggagcgc
     3181 gcgcctgagc gtcgggcgct gggcggactt cgtctcctgt tcgcgcacgg gaacgtagtt
     3241 gagcgtaaac gtttccccgg cgatgtttgc cgcgagcggc
//
LOCUS       DAOMGY010000109         3259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_31306_length_3259_cov_3.068352, whole genome shotgun sequence.
ACCESSION   DAOMGY010000109 DAOMGY010000000
VERSION     DAOMGY010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31306_length_3259_cov_3.068352"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..973)
                     /locus_tag="PLR57_02485"
     CDS             complement(161..973)
                     /locus_tag="PLR57_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HQQ40350.1"
                     /translation="MKIDRLNEMEAYIEKSGAISLQELCRCFHVSMSTVRRDVSELIR
                     RKRIQKIYGGVIRGSKTAADAAEIWAPQPFRAGSQTGFLAASLVREGMSVFLDSGALE
                     LIPYLAEKRHITVITNSLMVLKQAALYHTLNFIALGGIYNCGTASFSGQAALDELAKM
                     SIDLVFLSADGVTLERGLTSSNYTEIDVKKNVAKWNRDLVLLADGSVFGKNALITFCE
                     IARLRAIVCDRPLPSEFLRANALHKITILSPATEFETALEEPALERAYATNR"
     gene            complement(1064..2083)
                     /gene="iolG"
                     /locus_tag="PLR57_02490"
     CDS             complement(1064..2083)
                     /gene="iolG"
                     /locus_tag="PLR57_02490"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HQQ40351.1"
                     /translation="MIQIGIIGAGRIGQVHMKSVASGVPNAVIRSLADPMISAQAEQM
                     ARGFGIEHICGDYHEILEDLCIDAVLVCSPTDIHAKISVEAIRAGKHVFCEKPVDHEL
                     AKIDFVEEALKASPKKLKYQVGFNRRYDHNFRALRQAVLEGKVGDVQFIRVSSRDPQP
                     PHAGFIASSGGLFLDMMIHDLDMIRYLSGSEVEEVFAQGAVLIDPEIGAAGDVDTATV
                     SARMTNGALALIDNSRKAVYGYDQRAEVFGSKGCVQNANDLPSTVVLSTVDGVTAEKP
                     LWFFLERYMASYQAEVRAFIDSIEQDTETEVGIFDGRRTIQIALACKKSLAENRPVRV
                     DEIKA"
     gene            complement(2143..>3259)
                     /locus_tag="PLR57_02495"
     CDS             complement(2143..>3259)
                     /locus_tag="PLR57_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate mutase
                     (2,3-diphosphoglycerate-independent)"
                     /protein_id="HQQ40352.1"
                     /translation="GVAFDTVPALSLPKVAEQVAEGMQKGFDFIVTNFANGDVIGHTS
                     NNAAKVETARVVDKYLGETLAKAKAAGYTTLITADHGNLERMITPDGKADVAHTENLV
                     AFILVPPEGTAPAIARTLFDPNRADGALCDVTPTVLEALGVQKPADMLGKPQFRPEKT
                     GKVLLIILDGWGMGAQDETNPIFLAETPVWDDLLKNYPVRYLRASGNAVGLESGKAGN
                     SEAGHLNIGAGRVVPQDDVRLENAMQDGSFGENPVFISAVEQAKQTGKAVHLFALLTK
                     KSSHGSIDYPLELLGLCKRMEMENVFVHIFFDGRSTPPGSAPQLLRELEEKMRQIGAG
                     TIVSGVGRGIALDRDKNWAKVKKAYDSLTLGKGARYR"
BASE COUNT          655 a          983 c          888 g          733 t
ORIGIN      
        1 tacagccaca tgaatcacgt atcggacaag ggcgatatgt gcgccgacct gctgcaggat
       61 atcgtagtca agctgtaaga aaacacaagc caaacgaaaa gcccctcgag aatgcgcttc
      121 ccgggggctt tgtttttaac ctgtttgcgt ttttcgctcg tcagcggttc gtggcgtacg
      181 ccctttccaa agcgggttct tccagcgcgg tttcaaactc ggttgcaggc gaaagaatgg
      241 taattttgtg cagagcattg gcgcgcaaaa attcgcttgg cagcggacgg tcgcaaacga
      301 ttgcccgcaa gcgcgcaatc tcgcagaatg tgatcaacgc gtttttgccg aacacggaac
      361 cgtccgccag caacaccagg tcgcggttcc atttggctac gttcttcttt acgtcgatct
      421 ctgtatagtt ggagctggtc agcccgcgtt ccagcgtcac gccgtccgcg gagaggaaca
      481 ccagatcgat ggacatcttc gccagctcgt ccaacgcggc ctgaccggaa aacgaagccg
      541 tgccgcagtt gtagattccg ccgagcgcaa tgaaattgag cgtgtgatag agcgccgcct
      601 gtttaagtac catcaggctg ttggtgatga ccgtaatatg tcgcttttcg gcaagatacg
      661 gaataagctc cagcgcgccg gagtcaagaa acacactcat cccttcccgc accagcgaag
      721 ccgccagaaa cccagtttgg cttcccgccc ggaaaggctg aggagcccag atttccgccg
      781 cgtctgccgc cgtctttgag ccgcggatta cgccgccgta gattttttgt atgcgtttgc
      841 ggcgaatcag ctcggagacg tcccttcgca ccgtgctcat ggaaacatga aagcaacggc
      901 acaactcctg cagcgaaatt gcgccggatt tttcgatgta tgcttccatt tcgttgagcc
      961 ggtcgatctt cacaaaaaga acctccgctc gtaaaatctg tttagcgagc cgctttgcct
     1021 gttccgtctt gataatcgta aagcgccgct tcgttcgact gcgttatgcc ttgatttcgt
     1081 ccacgcgaac cgggcggttc tcggcgagcg actttttaca ggccagcgcg atttgaatcg
     1141 tcctgcgacc gtcaaaaatg ccgacttccg tctcggtgtc ctgctcgatg ctgtcgataa
     1201 acgcgcgcac ttccgcctga taggacgcca tgtaccgttc caggaagaac caaagcggtt
     1261 tttccgccgt cacgccgtcc accgtggaaa gtaccactgt cgagggcaaa tcgtttgcgt
     1321 tctgcacgca gcctttcgaa ccgaacacct cggcgcgctg gtcgtaacca tagaccgcct
     1381 tgcgggagtt gtcgatgagt gcaagcgcgc cgtttgtcat gcgcgcggaa acggtcgcgg
     1441 tgtccacatc tcccgccgcg ccgatttcgg ggtcgatcag caccgcgccc tgcgcgaaaa
     1501 cttcctcgac ctcgctgccg gagagatagc gaatcatatc caagtcgtga atcatcatat
     1561 ccagaaacag cccgccggag gacgcgataa agcccgcgtg cggcggctgc ggatcgcgcg
     1621 agcttacgcg gataaactgc acgtccccga ctttgccttc gagcaccgcc tgacgcagcg
     1681 cgcggaagtt atggtcgtag cggcggttga agccgacctg atacttgagc tttttaggag
     1741 atgccttcag tgcttcttcc acaaaatcga tcttggcgag ttcgtgatcc acaggctttt
     1801 cgcagaatac atgcttgccc gcgcggatcg cttcaacgga tatcttggcg tggatatccg
     1861 taggcgagca gacgagcacc gcgtcgatgc agaggtcttc cagaatttcg tgataatcgc
     1921 cgcagatgtg ctcaatgccg aaacctcttg ccatctgttc cgcctgcgcg ctgatcatgg
     1981 ggtcggcgag cgagcgaatc accgcgttcg gcacgccgga agcaacgctt ttcatatgta
     2041 cctgaccgat gcgccccgcg ccgatgatgc caatctgaat catcttaact cctcgctttc
     2101 attctgtttc tccagaagaa aattaaagca aatttctcgc gctcagcggt accttgcgcc
     2161 cttgccgagg gtcagggaat cgtaagcctt tttcactttt gcccagttct tgtcccgatc
     2221 cagcgcgatg ccgcgcccga cgccggaaac gattgtgcct gcgccgattt ggcgcatttt
     2281 ctcttccagc tcgcgcagca attgcggcgc gctgcccggc ggcgtggagc gcccatcgaa
     2341 gaagatatgc acaaacacgt tttccatctc catacgcttg caaagaccca gcagttccag
     2401 cggatagtca atcgacccgt gggaggactt cttagtcagc agcgcaaaca ggtgcacggc
     2461 cttgccggtt tgctttgcct gttcgacggc ggaaatgaac acgggatttt cgccaaacga
     2521 gccgtcctgc atagcgtttt ccagccggac gtcgtcctgc ggcaccacgc gccccgcgcc
     2581 aatgttcaaa tgtcccgctt cggagttgcc cgccttgccg ctttccagcc cgacagcgtt
     2641 tccggaagcg cggagatagc gcacaggata gttttttaac aggtcgtccc agaccggcgt
     2701 ttccgctagg aaaatcgggt tggtctcgtc ttgtgcgccc attccccagc catccagaat
     2761 gatgagcagt actttgcctg tcttttccgg acggaactgc ggcttgccaa gcatatcggc
     2821 tggcttttga acgccaagag cctccagcac ggtcggagtt acgtcgcaaa gcgcgccgtc
     2881 cgcgcggttc gggtcgaata aagtccgcgc tatggcaggc gcggttcctt ccggcggaac
     2941 caagatgaac gccacaaggt tctcggtatg cgcaacgtcc gccttgccgt ccggcgtaat
     3001 catccgctca agattgccgt ggtcggcggt gatcagggtc gtataacccg ccgctttcgc
     3061 ctttgcaagc gtttcgccaa ggtatttgtc cacgacgcgc gcggtctcca ccttggcggc
     3121 gttgttgctg gtgtgcccga tcacgtcgcc gttggcgaag ttggttacga taaagtcaaa
     3181 ccctttttgc atgccttccg caacctgctc cgccaccttc ggcaggctca gcgcgggcac
     3241 ggtatcgaac gcaacgccc
//
LOCUS       DAOMGY010000110         3250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_31418_length_3250_cov_3.380282, whole genome shotgun sequence.
ACCESSION   DAOMGY010000110 DAOMGY010000000
VERSION     DAOMGY010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31418_length_3250_cov_3.380282"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            516..2162
                     /locus_tag="PLR57_02500"
     CDS             516..2162
                     /locus_tag="PLR57_02500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HQQ40353.1"
                     /translation="MANKLKIIPLGGLGEIGKNMTVLEYGNDIVVIDCGLSFPDDDMP
                     GIDIVIPDMSYLRENRAKLKGFLITHGHEDHIGALPYALKEFDVPVYGTAFTVALIEH
                     KLEEARVSSKKLNVVKPGDFVELGCFKVEFIKTGHSIAGAVALAVNTPIGTVIHTGDF
                     KVDFTPIDGEPIDIIRFAHYGARGVLALMSDSTNVESPGHTQSERDIGKTFEYYFDVA
                     EGRIIVASFASNVYRIQQVADVAVSHGRVICFQGRSMEQVVQIAMRLNYLKIPAESIV
                     KVEQLKRYRDDQICVITTGSQGEPMSGLFRMATATHRLNIGKGDTVIISASAIPGNEI
                     GVSRVINQLYQKGAQVIYNRMADVHVSGHACREELRLMMELTKPKFFIPVHGEYRHLV
                     MHGKLAEDMGVLPDNTFIADCGDVIELTQRSGRKAGAVTAGAVMVDGIGDIDDVVLKD
                     RKTLSQDGLVVAVLTLDSETGRMLAAPEIVSRGFVYMRDSEELIAETKALLVKETRKF
                     EGADRSDYANIKSNIRASLKSFLKSKTKRTPMIMPIIIEI"
     gene            2176..2517
                     /locus_tag="PLR57_02505"
     CDS             2176..2517
                     /locus_tag="PLR57_02505"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HQQ40354.1"
                     /translation="MVYDTAHKLAEELKVSEEYVTYAAAREQAMENATTRALIDEYNK
                     LQIRAQAALISGDRENESVKKLQKIGELLQFDQTASAYLMAEFRLKRALGDVYKILAD
                     AVDVDLGALEG"
     gene            2684..>3250
                     /locus_tag="PLR57_02510"
     CDS             2684..>3250
                     /locus_tag="PLR57_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQQ40355.1"
                     /translation="MATRAEREKLKKQKRMRRLWLAVRPAVVLGISLLIVFFIARFTV
                     NYVLSNYINPVDMNDATPIEVTIPSSSSASSIARILYGACGYDKEGLISSTAAFKVYV
                     DFVGKANSLKAGTYVFSKNMSVKQIVEELCEGNAPKATVTFTVPEGYTIVGIGKVLIE
                     KGLISNEADLLEAARTGSAFTNFAFINDV"
BASE COUNT          816 a          874 c          942 g          618 t
ORIGIN      
        1 accattccgg acagcgtaat cacctatgcc gacgacgtgt tcaacctcgg caaccgtctg
       61 ttgccgagca acgtaatgtc gtttcgcagc gaaaacaccg ataaaacctg gggcaccaga
      121 acgttttacg gcacatacgg cggcaacgac ttttacagtt aattcagctc cctctgacga
      181 gggagctgtc gcccgcaggc gactgaggga gagaaaaaga agcattcttt ctccctccgg
      241 cacctttcgc ggcaaagcgg cagaaacgcc agttaacaca gaacgttcgc ggaacagtgt
      301 tccgcttttg gggaaaaacg aacccgccgg gcgggtaaaa ccagaaaaca acgtcaagct
      361 tgcgcaaggc aagccttact gttgattttg gggatcagtg agaaacacat atatcgcccg
      421 gataggcgaa aagtacacag aattgaactt gtgcggggca tgcgtgcctg cgctttgggg
      481 ataggatgaa aaagagtatt ggagaagaat aaaaaatggc taacaaatta aagattatac
      541 cgcttggcgg attgggcgag atcggcaaaa acatgaccgt tctcgagtac ggaaatgata
      601 tcgtggtcat cgactgcggg ctcagctttc cggacgacga tatgcccggc atcgacattg
      661 tcattccgga catgtcttac ctgcgcgaaa accgcgcaaa actgaaaggg tttctaatta
      721 cccacggaca tgaggatcat atcggggcgc tgccgtacgc gctcaaggaa ttcgacgttc
      781 ccgtttacgg cacggcgttt acggttgcgc tgatcgagca taagctcgag gaagcgcgcg
      841 tatcaagcaa aaagctcaat gtcgtcaagc ccggcgactt cgttgaactt ggctgcttca
      901 aggtggagtt cattaaaacc gggcattcaa tcgcgggcgc ggtcgcgctg gcggtcaaca
      961 cgcccatcgg cacggtgatt cacaccggag acttcaaggt ggacttcaca cccatcgacg
     1021 gcgagccgat cgacatcatc cgctttgcgc attacggcgc gcgcggcgtg ctcgcgctga
     1081 tgagcgacag caccaacgta gaaagcccgg gacacacgca gagcgagcgg gatatcggca
     1141 aaacgttcga atactatttc gacgttgcgg aagggcgcat catcgttgct tcctttgcca
     1201 gcaacgttta ccgcatccag caggttgcgg acgttgcggt cagtcacggc cgcgtgatct
     1261 gctttcaggg gcgcagcatg gagcaggtgg tgcagattgc catgcgcctg aactacctga
     1321 agattccggc ggagagcatc gtcaaggtgg agcaactcaa gcgctaccgc gacgaccaga
     1381 tctgcgtcat cactaccggc agccagggcg aacccatgag cggtctgttc cgcatggcaa
     1441 ccgcgacgca ccgcctcaac atcggtaagg gcgacacggt gatcatctcc gcctcggcca
     1501 ttccgggcaa cgagattggc gtttcccgcg tcatcaatca gctttaccaa aaaggcgcgc
     1561 aggtcattta caaccgcatg gcggacgtgc atgtttccgg tcacgcctgc cgcgaggaac
     1621 tgcgcctgat gatggaactg acgaaaccca agttctttat tcctgtgcac ggcgagtacc
     1681 ggcatttggt gatgcacggc aaacttgccg aggacatggg cgttttaccg gacaacacgt
     1741 ttattgcgga ctgcggggac gtgattgaac tcacccagcg gagcgggcgc aaagcgggcg
     1801 ccgtcaccgc gggcgcggtc atggtggacg gcatcgggga catcgacgac gtggtgctca
     1861 aagaccgcaa gacgctttcg caggacggcc tcgtggttgc ggtgcttacg ttggacagcg
     1921 aaaccgggcg catgcttgcc gctccggaaa ttgtctcccg cgggtttgtg tatatgcgcg
     1981 attccgagga gctgattgcg gaaaccaagg cgctgctcgt caaggagacc cgcaagttcg
     2041 aaggcgcgga ccgcagcgac tacgcaaaca tcaagagcaa tatccgcgct tcgctcaagt
     2101 cgtttctaaa gagcaaaacc aaacgcacgc cgatgatcat gccgatcatc attgaaatat
     2161 aggagagagc gaaacatggt ttacgacacg gcgcacaaac ttgccgagga actcaaggtg
     2221 agcgaggaat acgtaacata cgcggcggcg cgcgaacagg caatggaaaa cgcaaccacg
     2281 cgcgcgctga tagacgagta caacaagctc cagattcgcg cgcaggcggc gctgatttcc
     2341 ggcgaccgcg aaaacgaatc ggtcaagaag ctccagaaga tcggcgaact gctgcagttc
     2401 gatcagaccg cgtccgctta cctgatggcg gagttccgcc tcaagcgcgc gctgggggac
     2461 gtatacaaaa tcctcgccga cgcggtggac gtcgatctcg gcgcgctgga gggctgagtt
     2521 cacaacacgt tcataaactt gaactattta aacaggattg ctatactgat gcggacgaga
     2581 aaccgcgttt aagaagccgc gcattatgaa ccacggttcg gatggaacaa gcaggaacga
     2641 gtgcatagcg acaacgtaag ataaggaaga gcgagacccg actatggcaa cgagagcgga
     2701 acgcgaaaaa ctgaagaagc aaaagagaat gcgcaggctc tggctggcgg ttcgtccggc
     2761 ggttgtgctg ggcatcagcc tgctgatcgt gttctttatt gcgcgtttta ccgtaaacta
     2821 cgtgctttcg aactatatca accccgtcga catgaacgac gcgacgccca tcgaggtcac
     2881 aattccgtcc tcctcgtccg ccagcagcat tgcgcgcatt ctctacggcg catgcggcta
     2941 cgacaaggaa gggctgattt ccagcaccgc ggcgtttaag gtgtatgtgg actttgtcgg
     3001 taaggcaaac agcctcaagg cgggcacgta tgtgttcagc aagaacatga gcgtcaagca
     3061 gatcgtagaa gagctctgcg aaggcaacgc gccaaaagcc acggtcacct ttaccgtgcc
     3121 ggaaggctac accattgtcg gcattggaaa agtgctcata gaaaagggcc tgatttcgaa
     3181 cgaagccgat ttgctggagg ctgccagaac cggcagcgcg tttacgaact ttgcgttcat
     3241 caacgacgtt
//
LOCUS       DAOMGY010000111         3248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_31455_length_3248_cov_2.435954, whole genome shotgun sequence.
ACCESSION   DAOMGY010000111 DAOMGY010000000
VERSION     DAOMGY010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31455_length_3248_cov_2.435954"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="PLR57_02515"
     CDS             complement(<1..1293)
                     /locus_tag="PLR57_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQQ40356.1"
                     /translation="MRQRIFWNIFLASVIAAVLLGGAVLVTQYDVFEERIFSELESEC
                     GYVSAGILQAADETAYLDSLSSKNRITLINPDGSVAYDSEEDETTLDNHATRPEVAQA
                     LVAGTGQSSRYSETQLTRNFYYAVRISDGRVLRVSTAQKSLLGMVGSMLPTILLGILA
                     ILIISVFFSRYAAKRIVAPINALDLEKPLGNAAYDELAPLLTNMEHQRREIAARIGAL
                     CEKQREFTAVTEHMREGLILLDSSERMLAINSSAAHTFGADASAVIGAHVLMLNRSYA
                     LQQALERAKTGGAGESTLIKGAKTYQVLASTVEDESVAAGTVILILDVSDKVAAEQMR
                     REFSANVSHELKTPLTSILGYAEIMRDGVAKPEDTRRFAGKIHSEAAQLMSLIEDIIR
                     LSQLDEKTALPEMTTVDLKAICEDVCQRMMPQAQKAGVT"
     gene            complement(1409..2077)
                     /locus_tag="PLR57_02520"
     CDS             complement(1409..2077)
                     /locus_tag="PLR57_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQQ40357.1"
                     /translation="MIYYVEDDANIRELVVYTLSQMGLEARGFVDAASFFAAIQEQKP
                     SLALLDIMLPGDDGLTILKKIRSSSATADLPVIMVTAKSAEYDKVSGLDLGADDYIAK
                     PFGMAELVARVKALLRRSGAKSGSDRLVSGDLIVDVKRHTVTAGKNDIALTFKEFSLL
                     ACLMENAGRAFTREQLLETVWGYDYEGGTRTVDVHVQTLRTKLGECGKQVETVRGVGY
                     RFHD"
     gene            complement(2111..2764)
                     /gene="phoU"
                     /locus_tag="PLR57_02525"
     CDS             complement(2111..2764)
                     /gene="phoU"
                     /locus_tag="PLR57_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005345595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQQ40358.1"
                     /translation="MRITFEEQLRLLNQELLEMGALIEHAIQSASEALIKQDVDAANA
                     AIAFDREVDQKERDIETLCLKLLLQQQPVARDLRLISAALKMITDMERIGDQASDISG
                     IVIYLAGKPYIKALEHLPQMAEASIRMVSGSIDAFINKDLALARNVIEMDDIIDDLFD
                     VIKGELIELIQRDVTNGEQAIDLLMIAKYFERIGDHAQNIAEWAEFSITGVHKGAEL"
     gene            complement(2834..>3248)
                     /locus_tag="PLR57_02530"
     CDS             complement(2834..>3248)
                     /locus_tag="PLR57_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ40359.1"
                     /translation="RGKAKLDDIVEQSLRDAAIWEELKDRLKKDALGLSGGQQQRLCI
                     ARALAVEPEVLLMDEPTSALDPISTSRIEDLCVTLKEKYTIIIVTHNMQQAARISDNT
                     AFFLLGEVIEYGSTEQIFSIPRDKRTEDYITGRFG"
BASE COUNT          621 a          930 c          948 g          749 t
ORIGIN      
        1 cgtgacgccc gccttctgag cctgcggcat catgcgctgg cagacgtctt cgcagatggc
       61 ttttagatca acggtggtca tctccggcag ggcggttttt tcgtccagct gtgagaggcg
      121 gatgatgtct tcaatcaacg acatgagttg agcagcctcc gaatggattt ttccggcaaa
      181 gcggcgtgtg tcctccggct ttgccacacc gtcgcgcatg atctccgcat agccgaggat
      241 agaggtgagc ggcgttttca gctcgtggga tacgttggcg gaaaactccc ggcgcatctg
      301 ttcggcggca accttgtcgc tcacgtcgag aatcaaaatc accgtgcctg ccgcgacact
      361 ttcgtcctca acggtgctgg cgagcacctg ataggttttt gcgcccttga tcagcgtact
      421 ttctcccgcg ccgcctgttt tcgcgcgttc cagcgcttgc tggagggcgt agctgcggtt
      481 gagcataagc acgtgcgcgc cgatgaccgc acttgcgtcc gcgccaaagg tatgcgccgc
      541 gctggagttg atggcaagca tccgctcgct ggagtccagc agaatcagcc cttcgcgcat
      601 gtgctcggtt acggcggtga actcccgctg cttttcgcag agcgcgccga tgcgggcggc
      661 aatctcccgc cgctgatgct ccatgtttgt taatagcggc gcgagctcgt cgtaagcggc
      721 gttgccgagc ggcttttcca agtcgagcgc attgatgggg gcaacgatgc gctttgccgc
      781 gtagcgcgaa aagaacacgg agatgatgag aattgcgaga atccccagca gaatggtcgg
      841 cagcatggaa ccgaccatgc cgagcaggct cttctgcgcg gtggaaaccc gcagaacgcg
      901 cccgtcgctt atgcggacgg cgtaatagaa attccgcgtg agctgcgttt ccgaatagcg
      961 ggagctttga cccgtaccgg caaccagcgc ctgcgccact tccggacgcg tggcgtggtt
     1021 atccagcgtg gtttcgtcct cttccgaatc gtaggcaacg gagccgtcgg gattgatcag
     1081 cgtgatgcgg tttttggaag acaggctgtc cagatacgcg gtttcgtccg cggcctgcag
     1141 aatgccggcg gaaacatacc cgcattccga ttcgagttcg gagaagatgc gctcctcaaa
     1201 cacgtcgtat tgcgtcacca gcaccgcccc gcccaagagg acggcggcga tgacggaggc
     1261 gagaaagatg ttccaaaata tgcgctgtct catttcatcc tcgtttaagt gctatttcgg
     1321 tatgtttcaa gttacgcgcc gctgaaggaa cgctttagaa gaaacgattt tgctgcggag
     1381 aaagccgctg ttcggtggga agtttctgtt aatcgtggaa acgatagccc acgccgcgaa
     1441 cggtttcgac ctgtttgccg cattcgccaa gctttgtgcg cagggtttgc acatgcacgt
     1501 ccaccgtgcg cgttccgccc tcgtagtcgt agccccaaac cgtttcgagc agctgctcgc
     1561 gggtaaaggc acggcctgcg ttttccatta aacaagccag aagtgaaaac tccttaaacg
     1621 tcagcgcgat atcgttcttg cccgccgtta cggtgtgccg cttgacgtcc acaatgaggt
     1681 cgccgcttac cagccggtcg gaaccggact ttgcgccgct tctgcgcagc aaggccttca
     1741 cacgcgcgac cagctccgcc atgccgaagg gctttgcaat gtagtcgtcc gcgccgaggt
     1801 cgaggccgga aaccttgtcg tattccgcgc tctttgccgt gaccatgatc acgggcaaat
     1861 cggcggtggc ggaggaggag cggatttttt tgagtatcgt cagtccgtcg tcccccggca
     1921 gcatgatatc cagcagcgcc agcgagggct tttgctcctg tatggcggca aaaaacgagg
     1981 cggcgtcgac aaaaccgcgc gcttccagac ccatctggct gagcgtataa acaaccagtt
     2041 cgcggatgtt cgcgtcgtcc tcaacgtaat agatcatact caggctcctt tatgcgtgtt
     2101 gtttttcgcg ttacagctcg gcgcccttgt gcacgccggt aatcgaaaac tccgcccatt
     2161 cggcaatgtt ctgcgcgtgg tcgccgatgc gctcaaagta ctttgcgatc atgagcaggt
     2221 caatggcctg ctcgccgttg gtcacgtcgc gctgaatcag ctcgatcagc tcgcccttga
     2281 tcacgtcgaa gaggtcgtct atgatgtcgt ccatttcaat gacgttccgc gcgagcgcca
     2341 aatccttgtt gataaacgcg tctatgctgc cgctcaccat gcggatggaa gcttccgcca
     2401 tttgcggtag atgctcgagc gctttgatat acggcttgcc cgcgaggtag atcacgatgc
     2461 cggaaatatc cgaagcctga tcgccgatgc gctccatatc cgtaatcatc ttcaacgccg
     2521 cggagatcag ccttaaatca cgagcgacgg gctgctgctg gagcagcagc ttgaggcaga
     2581 gcgtttcaat atcgcgctcc ttttgatcga cttcgcggtc aaaggcgatg gcggcgttgg
     2641 cggcgtccac gtcctgcttg atgagcgctt cgctggcgct ctggattgcg tgctcaatga
     2701 gcgcgcccat ctcgagcagt tcctgattca gcaggcgcag ctgttcttca aatgtaatac
     2761 gcattaaata ttaccctccc gttgctgtta gatgacgtcg ttgaggaaaa caataccgat
     2821 gaattggact gcatcaaccg aacctcccgg tgatgtaatc ctctgtccgt ttatcccgcg
     2881 ggatgctgaa gatctgctcc gtggagccgt attcaatcac ttcgccgagc aggaagaagg
     2941 cggtgttgtc cgaaatgcgc gcagcctgct gcatattgtg cgttacgatg attatagtat
     3001 atttttcttt cagcgtcacg cagaggtctt caatgcggct ggtgctgatg gggtcgagcg
     3061 cgctggtggg ttcgtccatg agcagcactt ccggctcgac cgccagagcg cgcgcaatgc
     3121 agagcctttg ctgctgtccg ccggagaggc cgagcgcgtc ttttttgagg cgatccttga
     3181 gctcttccca gatggcggcg tcccgaagcg actgctcaac aatgtcgtcg agtttggctt
     3241 tgccgcgc
//
LOCUS       DAOMGY010000112         3243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_31530_length_3243_cov_2.693225, whole genome shotgun sequence.
ACCESSION   DAOMGY010000112 DAOMGY010000000
VERSION     DAOMGY010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31530_length_3243_cov_2.693225"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..889
                     /locus_tag="PLR57_02535"
     CDS             23..889
                     /locus_tag="PLR57_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQQ40360.1"
                     /translation="MKESTKGSLAMLAAALLWSLGGIFIKLVPWNPLSITGARGLLGG
                     IVVFIYLRIKKIPLAINKDTIKIAVSLACVCATFVTANKLTTAANAIVIQYCAPVYVL
                     LYVAFVQKKKLKPLDIAVVPITILGVALCFISQIGKGTLTGDLVAVVSGMFFAAMFIF
                     SEGVSDETRFNGIMQGQLLTALIGLPVLFATRPAFTTQAMIGILVLGIFQIGIAYVLY
                     GVALKSAPILNCTLLAVIEPLLNPVWVFLFNGENPGIWSLVGGVIVVGVITLWYVKDS
                     KSATPNAGEISA"
     gene            1008..1370
                     /gene="rplS"
                     /locus_tag="PLR57_02540"
     CDS             1008..1370
                     /gene="rplS"
                     /locus_tag="PLR57_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQQ40361.1"
                     /translation="MSDIIRELDKEQIRTDLPELQVGDAVRVFVKVIEGNRERLQNFE
                     GVVIKIQGGGIRKSFTVRRISYGVGVERTFPYNSPRIGRIEVVHHGVVRRAKLFYLRE
                     RTGKAAKIRERITEKSSK"
     gene            1475..2329
                     /gene="ylqF"
                     /locus_tag="PLR57_02545"
     CDS             1475..2329
                     /gene="ylqF"
                     /locus_tag="PLR57_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HQQ40362.1"
                     /translation="MPAINWYPGHMAKTRRMLVESLKMIDVVVEIIDARAPLASRNPD
                     FDDLFAGRARVVLLNKSDLADPQATKRWISYYASRGVAAAGISATGGGAKKTAVTLIE
                     RAAKPKVDAMRLKGVNKTVRCIVVGIPNVGKSTFINRMAGETRAEVGDKPGVTRGKQW
                     VKITPYLELMDTPGMLWPKLENQELASHLAFLGSIKDEIMDSEALATDLLKLLQVSSP
                     APLTERFSKLAPETPTEDLLDAVCRSRGFLLKGGALDTERAAHVALDEFRAGKIGRVT
                     LELPEARA"
     gene            2326..2955
                     /locus_tag="PLR57_02550"
     CDS             2326..2955
                     /locus_tag="PLR57_02550"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQQ40363.1"
                     /translation="MTRLPEEERLNRLAEPDRAFWEREGILLAGMDEVGRGPLAGPVV
                     VCCAAMPHSPLIKHVNDSKKVSEPRREALYSELAETALGFSFGWVWPETIDEINILQA
                     TRQAFREAFLGMPVTVTDVLVDAMKGLDIPARQHPLIHGDALSYSIACASILAKVTRD
                     RYMLEQDALYPEYGFARNKGYGTSEHIMALKRFGPCPIHRRSFIGKFLT"
     gene            2993..>3243
                     /locus_tag="PLR57_02555"
     CDS             2993..>3243
                     /locus_tag="PLR57_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQQ40364.1"
                     /translation="MTTTSIGRRGEDLALRYLEKLGYVLLARNYRDGSREIDLVLGDG
                     DTIVFTEVKARSGIGFGTPAEFVGREKQRQLTRAAQTYL"
BASE COUNT          700 a          927 c          950 g          666 t
ORIGIN      
        1 cagagccctc gagttaataa gtatgaaaga gtccactaaa ggttctctcg ccatgctggc
       61 ggcggcgctg ctctggagct tgggcggcat cttcattaag cttgttccct ggaacccgct
      121 ttccattacg ggcgcgcgcg gcctgctcgg cggaatcgtt gtgtttattt acctccgcat
      181 caaaaagatt ccgcttgcga tcaacaagga cacgatcaaa attgcggtct ctcttgcctg
      241 tgtctgcgca acgttcgtta ccgcaaacaa gctcaccacg gctgccaatg cgatcgtaat
      301 tcagtactgc gcgccggtat acgtgcttct ttacgttgcg ttcgtgcaga aaaagaagct
      361 caagccgctc gatatcgcgg tggtgccgat taccattctg ggggtcgcgc tctgctttat
      421 cagccaaatc ggcaaaggta cgctgacggg cgacctcgtt gcggtggtat ccggcatgtt
      481 ctttgcggca atgttcatct tcagcgaggg tgtgagcgac gaaacgcgct tcaacggcat
      541 tatgcagggg cagctcttaa ccgcgctgat cggcctgccg gtgctgtttg ccacgcgtcc
      601 ggcgttcact acgcaggcga tgatcggcat tctggttctc ggtattttcc agatcggaat
      661 agcgtacgtg ctttacggcg ttgcgctcaa gagcgcgccc attttaaact gcacgctgct
      721 ggcggtcatc gaaccgctgc tcaaccccgt ttgggtgttt ctcttcaacg gcgagaatcc
      781 cggcatctgg tcgctggtcg gcggcgtaat cgttgttggc gtaatcacgc tctggtatgt
      841 aaaggactca aaaagcgcga ccccgaacgc cggcgagatt tccgcataaa cgccgaaaaa
      901 cggcttgcaa tcggcaatta cctatgttat aatcgcgttc gtgacatcgg gcggtcctct
      961 gttcggcatc ggataagaac gcttgcaaac tgaaaggaga attttcaatg tcggacatca
     1021 tcagagaact cgataaggaa caaatccgga cggaccttcc ggaacttcag gttggcgacg
     1081 cggttcgcgt tttcgtcaag gttattgaag gcaaccgtga acgtctccag aacttcgaag
     1141 gcgtcgtcat caagattcag ggcggcggca tccgcaagtc cttcaccgtt cgccgcatct
     1201 cttacggcgt aggcgtggag agaaccttcc cctacaacag cccgcgcatc ggccgcatcg
     1261 aagttgtgca ccacggcgtc gttcgccgcg caaagctctt ctatctgcgt gagcgcaccg
     1321 gcaaagccgc caagattcgc gaacggatca cggaaaagtc ttccaaataa gagacattct
     1381 tcgtaagata aaaccccctg ccggaaacgg caaaggggtt tttccgtatt taccgagcgg
     1441 ggaaatgcgt ttcacccgcc gaaaggaaac acctatgccc gcaattaact ggtatcccgg
     1501 acacatggcc aaaacgcgcc gcatgctcgt tgaaagcctc aaaatgatcg acgtggtggt
     1561 ggaaattatc gacgcgcgcg cgccgctagc ttcccgcaac ccggactttg acgacctgtt
     1621 cgccggcagg gcgcgggtcg ttctgctcaa caagagcgac cttgccgacc cgcaggcaac
     1681 caaacgctgg atttcttact acgcttcccg cggggttgcg gcggcgggca tcagcgcaac
     1741 cggcggcgga gcaaagaaaa ccgcagttac gctcattgag cgcgcggcaa aacccaaggt
     1801 ggacgccatg cgcctaaaag gcgtcaacaa gaccgtacgc tgtattgttg tcggcattcc
     1861 gaacgtaggc aaatcgacgt tcatcaaccg catggctggc gaaacccgcg cagaagttgg
     1921 cgacaagccc ggcgtcaccc gcggcaagca gtgggtaaag attacgccgt atctggagct
     1981 gatggatacg ccgggcatgc tctggccgaa gctggagaat caggaactcg cttcccatct
     2041 ggcttttctg ggttctatca aggacgaaat catggattcc gaagcgcttg ccacggatct
     2101 gctcaaactt ttgcaagtca gctcgcccgc gccgcttacg gagcgcttca gtaagctcgc
     2161 gccggaaacc ccgacggaag acctgctcga cgcggtctgc cggagcaggg gcttcctgct
     2221 caagggcggt gcgctggata ccgaacgcgc ggcgcatgtc gcgctggacg aattccgcgc
     2281 gggcaaaatc ggcagagtca cgctcgaact tccggaggcg cgcgcatgac gcgcttgccg
     2341 gaagaggaac gtctcaaccg tcttgccgaa cccgaccgcg cgttttggga gcgggaaggc
     2401 attctgctgg cgggaatgga cgaagtgggg cgggggccgc tggcggggcc ggtggtcgtg
     2461 tgctgcgcgg caatgccgca tagcccgctg ataaaacatg tcaatgactc caagaaggtg
     2521 agtgaaccac gcagggaagc gctgtattcc gaactggcgg aaaccgctct cggcttttct
     2581 ttcggctggg tctggccgga aaccatagac gaaatcaaca ttctgcaggc gacgcgacag
     2641 gcgtttcggg aagcgtttct gggcatgccg gttacggtga ccgacgtgct tgttgacgcc
     2701 atgaaaggcc tcgacatccc cgcgcgtcag cacccgctga tccacggcga cgcgctgagc
     2761 tactccattg cctgcgcttc cattctggca aaggtaacgc gggatcgcta catgctcgaa
     2821 caggacgcgc tgtatcctga atacggcttt gcgcgcaaca aaggctacgg cacttcggag
     2881 catatcatgg cgctcaagcg cttcggcccg tgcccgattc accgcagatc gtttatcggc
     2941 aagtttctga cttgaaccaa agagtgactt taatcagaag acgggggagc atatgacgac
     3001 tacctcgatt ggcaggcgcg gcgaagacct tgcgctgcgc tacctcgaaa aactgggcta
     3061 cgtcctgctg gcgcggaact accgcgacgg ttcgcgcgag atcgaccttg ttttggggga
     3121 cggggacacc atcgtcttta ccgaggtcaa ggccagaagc ggaatcggct ttggtacgcc
     3181 cgcggagttc gtgggccgcg aaaaacagcg tcagctgacg cgcgcggcgc agacgtattt
     3241 aat
//
LOCUS       DAOMGY010000113         3170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_32563_length_3170_cov_3.215730, whole genome shotgun sequence.
ACCESSION   DAOMGY010000113 DAOMGY010000000
VERSION     DAOMGY010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32563_length_3170_cov_3.215730"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLR57_02560"
     CDS             complement(<1..742)
                     /locus_tag="PLR57_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQQ40365.1"
                     /translation="MSDFNFHVKPIGLFDMLHFKKMVPARQKALACGSTDHVTGVYGS
                     NKLASRLHPKEQHLTIAEIIEHGENAKSFVLTGKNLAQFRAGQYVSVSLKIGSAVLTR
                     PYSISCSPKWALEGKYALTIKRQQDGFASGWILDNWKVGDEITVSGPEGTFYYEPLRD
                     AKHVVGLAGGCGITPFLSIAYAIRDGVEDFRLTLLYGSRKECDILYHSELEEIAKATG
                     KVKIVHILSDEKKAGYEYGFITAEAIQAA"
     gene            complement(739..2274)
                     /locus_tag="PLR57_02565"
     CDS             complement(739..2274)
                     /locus_tag="PLR57_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQQ40366.1"
                     /translation="MEHAYDAVVIGAGNGGLTAAATMAKAGKKVLLVEQHNLPGGFAT
                     SFVRGRFEFEPSLHELCDVGSEENPGEVRRLLQQTLGISVGWAAVPEAYRLLTIAEGK
                     DNLDIVMPFGVKEYVDQMEAYVPGSRPSMEKFIKLCEGIMAALAYIGASGGKADSKVL
                     MKEHANFLKCGGYSVSRVLKSLHMPKKAQRILCAYWCYVGISTDQLNFALFAAMLYKY
                     LTKGAWIPKMRSHELSVALDARIRELGGEIWYNTRAEKILTENGKIVGVRTSHGDVKT
                     SHVIACCSPNAVFGDMMDAADVPERQTRMVNARRIGARGFVVYLGLNKTADELGLTDY
                     SYFIYESMDTIKEAKRADGFNDHPMQATVCLNRANPGCSPEGTCIMSFTTLYNTDVWG
                     DVAPEDYVNKKREVAEKMIRDFEAHVGVSITPYIEEIEIATPVTMARYTLNPQGAMYS
                     YSCADWDTMLPRLMMLKEDQIVPGVRFAGGYGPRIYGYSSTYLNGELTAKLTLADMKE
                     AAK"
     gene            complement(2333..>3170)
                     /locus_tag="PLR57_02570"
     CDS             complement(2333..>3170)
                     /locus_tag="PLR57_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQQ40367.1"
                     /translation="FVSDWLFQSLQEGAELSATGPHGDFCVSPIRDTHEIVAIAGGAG
                     VTPFVSMMKQFLAGRPELSVTLLYGCRNPQDILFEQEIDAVIQADPKRFRRFNTFEDN
                     GGNASLRKGYLDAAFIRENIPEPEKKTYFICGPAIMYSFLEKEFPKVAFLERKQTRYE
                     VSGAPCDVTRIAGYPAVFAEKMYTVSVLCNEVKTEIPASGAEPLLTAIERAGLILDSR
                     CRSGECGICRSQLVSGDVFILPDGDGRREADKELGFIHPCATYPLSDLEVILPPGSAA
                     QT"
BASE COUNT          591 a          936 c          953 g          690 t
ORIGIN      
        1 aggcggcctg aatcgcttcg gcggtgataa agccgtactc gtagcctgcc ttcttttcgt
       61 cgctgaggat atgcacgatc ttgaccttgc cggtcgcttt ggcaatttcc tccagctcgc
      121 tgtggtagag gatgtcgcat tccttgcggc tgccgtagag aagcgtcagg cggaaatcct
      181 ccacgccgtc gcggatggcg tacgctatgg agagaaacgg cgtaatgccg cagccgcccg
      241 cgaggccgac gacgtgtttt gcgtccctga gcggttcgta atagaaggtt ccttccgggc
      301 cggacaccgt gatttcgtcc ccgaccttcc agttgtcgag aatccagccg gaggcaaagc
      361 cgtcctgctg gcgcttgatg gtcagcgcgt acttgccttc cagcgcccac tttggcgagc
      421 aggaaatgga gtacggccgc gtgagtacgg ctgagccgat cttcagcgaa acggaaacgt
      481 actgccccgc gcggaactgt gcgagatttt tgcccgtgag cacaaacgac tttgcgtttt
      541 cgccgtgttc gattatctcc gcgatggtca aatgctgctc tttcgggtgc aggcggcttg
      601 ccagcttgtt ggagccgtaa acgcccgtca cgtggtcggt cgagccgcag gccagcgcct
      661 tttggcgcgc gggcaccatc tttttaaagt gcagcatgtc gaacaggccg atgggtttta
      721 catgaaagtt aaagtcgctc attttgccgc ctccttcatg tccgcaaggg tgagctttgc
      781 ggtaagttcg ccgttgaggt aggtcgagct gtagccgtag atgcgcggcc cgtacccgcc
      841 cgcaaaccgg acgcccggca cgatctggtc ttctttaagc atcataagcc gcggcagcat
      901 ggtgtcccag tcggcgcagg agtaggagta catcgcgccc tgcgggttga gggtgtagcg
      961 cgccatggtc acgggagttg cgatttcgat ttcctcgatg tacggcgtaa tgctgacgcc
     1021 cacgtgcgct tcaaagtcgc gaatcatctt ttcggccact tcgcgcttct tgttcacgta
     1081 gtcttccgga gcgacgtcgc cccagacgtc cgtattgtaa agcgtggtaa agctcataat
     1141 gcaggtgcct tccggcgagc agcccgggtt ggcgcggttg aggcagacgg ttgcctgcat
     1201 ggggtggtcg ttgaacccgt ccgcgcgctt ggcttcctta attgtatcca ttgactcgta
     1261 gataaagtag ctgtagtcgg tcagtcccag ttcgtcggcg gttttattga ggccgaggta
     1321 gaccacgaat ccgcgcgcgc cgatccggcg ggcgttgacc atgcgcgtct ggcgttccgg
     1381 cacgtcggcg gcgtccatca tgtccccgaa gacagcgttg ggcgaacagc aggcgatgac
     1441 gtgactggtt tttacgtccc cgtggctcgt gcgaacaccg acgattttac cgttttccgt
     1501 gaggattttt tccgcgcggg tgttatacca aatctcgccg ccgagttcac ggatgcgcgc
     1561 atccagcgca acggaaagct cgtgggagcg cattttggga atccacgcgc ccttggtcag
     1621 gtacttatag agcatggccg caaacagcgc aaagttgagc tgatccgtgc tgatgcctac
     1681 gtagcaccag taggcgcaga ggatgcgctg ggcttttttg ggcatgtgca gggactttag
     1741 cacacggctg acgctgtagc cgccgcactt taagaagttc gcgtgctcct tcatgagcac
     1801 cttggagtcc gccttgccgc cggaagcgcc aatgtaagca agagccgcca taatcccctc
     1861 gcagagcttg atgaacttct ccattgaagg gcggctgccg gggacgtacg cttccatttg
     1921 atcgacgtac tccttcacgc cgaacggcat gacgatatcg aggttgtcct tcccctcggc
     1981 aatggtgagg agacggtagg cttctggcac cgccgcccag ccgacggaga tgccaagcgt
     2041 ctgctgcagc agacggcgca cctcgccggg gttctcctcc gagcctacgt cgcagagctc
     2101 gtgcagcgac ggttcaaact caaaccgccc ccgcacaaag cttgttgcga atccgcccgg
     2161 caggttgtgc tgctcgacca gcagcacctt tttgcctgct tttgccatgg ttgcggcggc
     2221 ggtgagcccg ccgttgcccg cgccgatgac gaccgcatca tatgcgtgtt ccaatgtttc
     2281 aacactcctt tccaaattta ccataagagg tggttactca aaaacgcttt gctcaggtct
     2341 gcgccgcgct gcccggcggc agaatcactt ccagatccga aagcgggtaa gtggcgcagg
     2401 gatgaatgaa gccgagctcc ttatccgctt cccgccgccc gtcgccgtcc ggcaggatga
     2461 acacgtcgcc cgaaacaagc tggctgcggc agatgccgca ttctccgctg cggcagcgcg
     2521 agtcgagaat cagtcccgcg cgctcaatgg cggtcaacag cggttccgcg ccgctggcgg
     2581 gaatctcggt tttcacctcg ttgcagagca cggaaacggt atacatcttt tccgcaaaga
     2641 ccgccggata ccccgcaata cgggtcacgt cgcagggcgc gccggagact tcgtagcgcg
     2701 tctgcttgcg ctccaaaaac gccaccttcg ggaactcctt ttcgaggaag ctgtacatga
     2761 tcgcaggccc gcagataaaa tacgtttttt tctccggttc ggggatgttc tcgcggatga
     2821 acgccgcgtc aaggtaccct ttgcgcaggc tggcgtttcc gccgttgtcc tcgaatgtat
     2881 tgaacctgcg gaagcgcttg gggtcggcct gaatcaccgc gtcgatttcc tgttcaaaca
     2941 ggatatcctg cgggttgcgg cagccgtaga gcagggtaac ggagagctcc ggccggccag
     3001 caaggaactg cttcatcatc gagacgaacg gcgtaacgcc cgcgccgccc gcaatagcga
     3061 cgatttcgtg cgtgtcccgg atcggggaaa cacagaagtc cccgtgcggt ccggttgcgg
     3121 aaagctcggc tccttcctgc aagctctgaa aaagccaatc ggaaacgaag
//
LOCUS       DAOMGY010000114         3152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_32853_length_3152_cov_2.843074, whole genome shotgun sequence.
ACCESSION   DAOMGY010000114 DAOMGY010000000
VERSION     DAOMGY010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32853_length_3152_cov_2.843074"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..401
                     /locus_tag="PLR57_02575"
     CDS             18..401
                     /locus_tag="PLR57_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HQQ40368.1"
                     /translation="MLCETRFFRCDICGNLVGMIHASGAKMICCGQEMTELVPGSVDA
                     SHEKHVPVIKQNGDSITVHIGSVDHPMTEEHHIAWVYLQTTDGGQRKCLAVGGAPEVT
                     FALGGAKPVAAYAYCNLHGLWKADA"
     gene            543..1217
                     /locus_tag="PLR57_02580"
     CDS             543..1217
                     /locus_tag="PLR57_02580"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQQ40369.1"
                     /translation="MPFDVQSWGESFIRAVESAFGARIVCAGIQGSRARGEAKPSSDI
                     DTVLILDELSAEDLSVYRALLQTLPNTELICGFVSGRTELACWEPSELVSFYFDTLPL
                     KGDLEFLRPLLTAEAARRAVLDGACGIYHACCHNVLFDHSVEILQALYKAAFFVLRAK
                     VFSEGGTFFRREDKLIPNLCGADREVMERRAHLMEHAPEETELIAETDLLLRWSGALI
                     RVFSEA"
     gene            1551..2162
                     /locus_tag="PLR57_02585"
     CDS             1551..2162
                     /locus_tag="PLR57_02585"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HQQ40370.1"
                     /translation="MAAAVATKALASLLFILLGVLCARLASDRAYAVVILIGLAFGAV
                     GDVCLNLRHLVGSRGKAVFMAGIAAFMIGHLFYLFALISRAPKMLLWAIPACAIVSVL
                     MLRFILKRIEAQGAIRIFGIVYLVVVFQMMCCALGLLPLNPNNPAYILFAAGAVLFAA
                     SDVLLVLNQFGKRQYPAFRPMNLSLYYLGQLCIALTIAFMPSL"
     gene            2381..3076
                     /locus_tag="PLR57_02590"
     CDS             2381..3076
                     /locus_tag="PLR57_02590"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HQQ40371.1"
                     /translation="MKQEGLKKSFVHDMQQKIFSGELQIGQQLPPERELASQLDVSRS
                     LVNTGIIELENQGFVRIMPRQGTVVADYKRNGTLQVLVALMSCDTAIIDYPLFCNLVD
                     FRVLIECECARLASVHGTMNDFDTLLTLLHCVERAAKPLDAVEPLVRFHYVLTQISGN
                     AVYAMTYKSFESVVKRLVTQHLTIAPDLPKTVRLHEALLAALTAHDPEKSAQCMRLCL
                     LQGMNAIKKLYKP"
BASE COUNT          670 a          811 c          908 g          763 t
ORIGIN      
        1 caaggaggat atttactatg ttgtgtgaaa cccgtttctt ccgttgtgat atctgcggca
       61 atctggttgg tatgattcac gcgtccggcg caaaaatgat ctgctgcgga caggaaatga
      121 ccgaattagt acccggcagc gttgacgcga gccacgaaaa gcacgttccc gtcatcaagc
      181 agaatggcga cagcatcacc gtccatatcg gcagcgtgga tcaccccatg accgaagagc
      241 atcacatcgc ctgggtgtac ctgcagacca ccgatggcgg ccagcgcaag tgcctggcgg
      301 ttggcggcgc gcccgaagtg acctttgcgc tcggcggtgc aaaacccgtt gcggcctatg
      361 cttactgcaa cctccacggg ctctggaagg ccgacgcgta acgcctggaa caaccaactc
      421 cttatgaaga aaacgttccg ccctgaaaac gggcggaacg ttttttatac gctcttgcat
      481 ccgccaaatc tgtggtatac tgtcccggca agagaaaatt cagccatttg ggaggtggaa
      541 ccatgccgtt cgacgtacag tcctggggag agagttttat ccgcgcggtg gagagcgcat
      601 ttggcgcgcg catcgtttgc gcggggattc agggcagcag agcccggggc gaggcaaagc
      661 cttcgagcga tatcgacaca gtgctgattc tggacgagct gtctgcggag gatttgagcg
      721 tttaccgcgc gctgctgcaa acgcttccga ataccgaact gatctgcggg tttgtttccg
      781 gcagaacgga actggcctgc tgggagcctt ccgagctggt ttcgttttac tttgatacgc
      841 tgccgctgaa gggcgatctg gagtttcttc gcccgctttt aactgcagaa gcggcgcggc
      901 gcgcggtgct cgacggcgca tgcggaatct accacgcctg ctgtcacaac gttctgttcg
      961 atcacagcgt ggagattctc caagcgttgt ataaagccgc gtttttcgtt ctccgcgcaa
     1021 aggttttttc ggagggtggc acatttttcc gcagagagga caagctgatc cccaaccttt
     1081 gcggagccga ccgcgaagtg atggaacgcc gcgctcacct catggaacac gcgccggagg
     1141 aaacggaact cattgccgaa actgatttgc tgctgcgctg gtcgggcgcg ctgattcgcg
     1201 tattttccga agcgtaaatt ttttcgtgat atattcgtga ttttgtgcgc atgccccgac
     1261 tgaggataac gcttttttac atgaaacaat accacaatca cacaaataaa tttagtatcg
     1321 tggccacaaa gatatagcct aaaaagtatg attttggttg gaaacccgct caaaccgtct
     1381 ggtttgcttg tatttacaac ctgcaaactg gtataattct accagtggct ataccagcag
     1441 acaggcgaat aacgaggggg gctccgagta tgaatcctgt ttggattgtc gtcattttgg
     1501 ttggatgcgc cgtagaggct gtatttatca tactggaaca gaagaaaaat atggcagcgg
     1561 cggtagcgac aaaagcgctc gcttcgctgc tgtttatttt gcttggcgtt ctttgcgcaa
     1621 gattggcgag tgatcgcgcg tatgccgttg tcattttgat cggtttagcc tttggtgcgg
     1681 tgggagacgt ttgccttaac cttcgccatc tggtgggcag ccgcggcaag gctgttttca
     1741 tggcgggcat tgcggcgttt atgatcgggc atttgttcta tctgtttgcg ctgatcagcc
     1801 gcgcgccgaa gatgctcctt tgggcgattc ccgcctgcgc gattgtgtcc gtactgatgc
     1861 ttcgcttcat tctcaaacga attgaagcgc agggcgcaat acgcatattt ggaattgtat
     1921 atcttgtagt tgtttttcaa atgatgtgtt gcgcgctagg tctgctgccg ctcaatccga
     1981 ataacccggc atacatcctc tttgcggcgg gggcggtgct gttcgccgcg agcgacgtgc
     2041 tgcttgtgct caaccagttc ggcaaacggc agtatccggc attccgaccg atgaatcttt
     2101 cgctgtacta tttgggtcag ctctgcattg cgctgaccat tgcgtttatg ccgtcattgt
     2161 aataaagaag ctgctttgga gcaaataaga cgtaatatca gctgacagtt caagacgggc
     2221 gcgggcctgt cacaaggaat tgaaccggac ggttcgaaat cgcgctgccg ggctgcggaa
     2281 gacattggtt ccgcgcggca ggagtggaag taggttaccg gcgcagccag ccagcgcgcc
     2341 gccggtaaga gaggaaaccg atacggttca acgcatatat atgaaacagg aaggcctcaa
     2401 aaagtcgttc gttcacgaca tgcaacaaaa aatcttttcc ggcgaactgc agattggcca
     2461 gcagctcccg ccggagcgcg agctcgcttc ccagctggac gtcagccgga gtcttgtgaa
     2521 cacgggcatc atcgagctgg agaatcaagg ttttgtgcgc atcatgccgc gccagggcac
     2581 cgttgttgcc gactataagc gaaacggcac gctgcaggtg ctcgttgcgc tcatgagctg
     2641 cgatacggct atcatcgatt atccgttgtt ctgcaatctg gttgatttcc gcgtgcttat
     2701 cgagtgcgaa tgcgcgcgcc ttgccagcgt tcacgggacg atgaacgatt tcgatacgct
     2761 gctgacgctg ctgcactgcg tggagcgggc ggcaaagccg ctggacgcgg tggaaccgct
     2821 ggttcgcttt cattatgttt taacgcagat ttcgggcaac gcggtgtacg ccatgaccta
     2881 taagagcttt gagtcggtgg tgaagcgcct cgtaacccag catctgacca ttgcgccgga
     2941 cttgccaaag actgttcgcc tgcacgaagc gctgctcgcc gcgttgacag cgcacgaccc
     3001 cgaaaagagc gcgcagtgta tgcggttgtg tctgctgcag ggcatgaatg cgatcaagaa
     3061 gctctataaa ccctgaagcc ctgctggttc tttgcagaat ttgattttgc gcggtacgat
     3121 tgccgttttc ccgccggaat ttacccggcg gg
//
LOCUS       DAOMGY010000115         3145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_32956_length_3145_cov_2.658576, whole genome shotgun sequence.
ACCESSION   DAOMGY010000115 DAOMGY010000000
VERSION     DAOMGY010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32956_length_3145_cov_2.658576"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PLR57_02595"
     CDS             complement(<1..928)
                     /locus_tag="PLR57_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQQ40372.1"
                     /translation="MKRKLASLLLAVLLLLSALCGCSSTDSAAVHDGSGEEKPLIGMS
                     FDSFLIERWQRDRDIFVSTASELGAEVNVQIANGSANEQVSQIEYFIKKGVDAIVVVA
                     VDCYGLSDIVKKAHDAGILVISYDRLIMNADSDLYISFDNEMVGTMMAEALVANMPAG
                     SNIVSICGSPTDQNVAQANRGFDAVIAKSDLNIIVEEYAPNWLAETAYNVVNQAIADG
                     LTFDGVRCGNDDLASQAFLALSEHRLASKVALVGQDADLAACQRIVEGTQVMTVYKPV
                     EKLAKEAAEIVVQMISGQPLETTDTIFDGVYDV"
     gene            complement(925..2523)
                     /locus_tag="PLR57_02600"
     CDS             complement(925..2523)
                     /locus_tag="PLR57_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQQ40373.1"
                     /translation="MYKILLADDEGIELDALTFILEKNFPGQCAVETAKTGCDAVELA
                     ERFRPDIVVMDIRMPGINGIEAIQSIQAISPGIVFIVLTAYDKFEYAKEAISLSVFDF
                     LTKPVNRLVFADTIRRAMNKVEAERKARSAALKTRERMENMLPVLENSFVYLLLMQSD
                     DIGAYLRLCDLLGIDAPCATVLVLELTRLVGGDLLELDVKVSEVYTAIRTLVKESFNC
                     IVGPAMANRVIIVRPTATPENEYYERLLLIEQGRALAHRVEDKVGAECKLGIGSTVPM
                     ERLSDSYLQAVKAVKHCKGIVSHFNDLPIAPAYEYGYPIEREKHLEEMIQKGDAAAAS
                     ADAESFFQWMTDTYPEHDMAIRLKVLEFVMRAEFRAFHEGGMKYSFLDRDEYLQAVLA
                     AESYEELRAWFVRKIAESARNVTTKADEKANRIIAKARAYIDRSFCRELTLEEVSREV
                     HVSPYYFSKLFKEQTVDNFINYVTLKRIETAKQLLRDGRLNIKSICSEVGYSDPNYFS
                     RLFKRFEGVTPTEYREQVAKETSV"
     gene            complement(2633..>3145)
                     /locus_tag="PLR57_02605"
     CDS             complement(2633..>3145)
                     /locus_tag="PLR57_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ40374.1"
                     /translation="FFRYSVKHTGQTSTLAEEISQVETYLHIINVRFSNEIQYVKSIE
                     GPVERIVLPGVILQPIVENAINHGLQDVKWEKRITLSTREQGESIVITVTDNGKGMTP
                     EEIDGVLVGQPVAVELDGQQHRGTGLKNVIERLRIYYGIKDVFEIASEGTGRGTAVSI
                     IIPRHGSHVM"
BASE COUNT          592 a          881 c          934 g          738 t
ORIGIN      
        1 gcacgtcata cacgccgtca aagatggtat cggtcgtttc cagcggctgg ccgctgatca
       61 tttgaaccac gatttcggcg gcttccttgg cgagtttctc aacgggcttg tatacggtca
      121 tcacctgcgt gccttcgaca atccgctgac aggcggcaag atccgcgtcc tgacccacaa
      181 gggcaacctt gctcgcgagc cggtgctcgg agagcgcgag aaaggcctga ctggcgaggt
      241 cgtcgttgcc gcagcgcacg ccgtcgaagg tcagcccgtc cgcaatggcc tggttcacca
      301 cgttgtaagc ggtttcggca agccagtttg gcgcatattc ttcgacaatg atgttcaaat
      361 cgctcttggc gatgaccgcg tcgaaaccgc ggttggcttg agctacgttc tgatccgtcg
      421 gagagccgca gatgctgacg atgttgctgc ctgcgggcat gttggcaacc agcgcttcgg
      481 ccatcatggt gccgaccatc tcgttgtcga aggagatata caaatcgctg tccgcgttca
      541 tgatcagccg gtcgtaggag atcacgagga tgcccgcgtc gtgcgctttt ttgacgatat
      601 ccgaaagtcc gtagcagtcc accgcgacca cgacgatggc gtccacgccc tttttgatga
      661 agtattcaat ctgggagacc tgctcgttgg cgctgccgtt ggcaatttga acgttgacct
      721 ccgcgccgag ctcgctggcg gtggagacga aaatatccct gtcgcgctgc cagcgctcaa
      781 tcaaaaacga gtcaaaactc atgccgatga gcggcttttc ctcgccgctg ccgtcgtgca
      841 ccgcggcgga atccgtactg ctgcagccgc agagcgcaga taaaaggagc agcaccgcaa
      901 gcagcagaga ggccagtttt cttttcatac gctcgtctcc tttgcaacct gttcgcggta
      961 ttcggtgggc gtaacgcctt cgaagcgctt gaacagcctt gaaaaataat tcggatcgct
     1021 gtagccgact tcgctgcaga tgcttttaat attcagcctg ccgtcgcgca ggagctgctt
     1081 ggcggtttcg atgcgcttga gcgttacgta gttgataaag ttgtcgacgg tctgttcctt
     1141 aaagagtttg ctgaagtagt aggggctgac gtgcacctcc cgcgagactt cctccagcgt
     1201 gagctcgcgg cagaagctgc ggtcgatata ggcgcgcgct ttggcgatga tgcggttggc
     1261 tttttcgtcc gccttggtgg taacgttccg tgcgctctct gcaattttgc ggacgaacca
     1321 ggcgcgcagc tcctcgtagc tctcggcggc gagaacggcc tgcaggtatt cgtcccggtc
     1381 gaggaagctg tatttcatgc cgccttcgtg gaacgcacgg aattccgcgc gcataacgaa
     1441 ctccagcacc ttgaggcgga tggccatatc atgttcgggg taggtgtccg tcatccactg
     1501 aaagaacgat tctgcgtccg cgctggcggc tgcggcgtcg cccttttgaa tcatttcttc
     1561 gaggtgtttt tcacgctcaa tggggtagcc gtactcgtac gcgggcgcga tgggcaggtc
     1621 attgaagtgc gaaacaatcc ccttgcagtg cttgacggct ttgaccgcct gtaaatacga
     1681 gtcgctcaga cgctccatag gcaccgtcga gccgatgccg agcttgcact ccgcgccgac
     1741 cttgtcctcc acgcggtgcg ccagcgcgcg tccctgctca atgagtaaga gccgttcgta
     1801 gtactcgttt tccggcgttg ccgtcgggcg aacgatgatg acgcggtttg ccatggctgg
     1861 gccgacaatg cagttgaacg attccttcac gagcgtgcgg atggcggtat acacttccga
     1921 aaccttgacg tccagctcca gcaggtctcc gccgacaagg cgcgtcagct ccagtaccag
     1981 tacggttgcg cagggcgcgt cgattcccaa aaggtcgcag agccgcaggt aagcgccgat
     2041 gtcgtcgctc tgcatgagca gcaggtagac aaagctgttt tcgagcacgg ggagcatgtt
     2101 ttccatgcgc tcgcgggttt tcagcgcggc ggagcgcgct ttgcgctcgg cttccacctt
     2161 gttcatggcg cgccggatcg tatccgcaaa gacgaggcgg ttgacgggct ttgttaaaaa
     2221 gtcgaacacc gaaaggctga tggcctcctt ggcatactca aacttatcat aagcggtgag
     2281 cacgatgaat acgatgccgg gggaaatcgc ctgtatcgat tggatggcct ctatgccgtt
     2341 gatgccgggc atgcggatat ccatcacgac gatgtccggt cgaaaccgct cggcaagctc
     2401 caccgcgtcg caaccggttt tggcggtttc gacagcgcac tggccgggga aattcttttc
     2461 taaaatgaaa gtgagcgcgt cgagttcaat gccctcgtcg tccgctagaa ggattttgta
     2521 catgatgcct gctccattgc tttttggtag tttccggaag ggaattgcat tgctgcggaa
     2581 gaagattccg cgttcggttg gggaaagatt gatttgccgc ggcttgccgc cgttacataa
     2641 cgtggctgcc gtgccgggga atgataatgc tcaccgcggt gccgcgcccc gtgccttcgc
     2701 tggcaatttc gaatacgtcc ttgatgccgt aatagatgcg gagacgctcg atgacgtttt
     2761 tcagtcccgt tccgcggtgc tgctgaccgt ccagttcaac ggcgaccggc tggcctacaa
     2821 gaaccccgtc gatttcctcc ggggtcatgc ctttgccgtt gtcggttacg gtgatgacga
     2881 tgctctcgcc ctgttcgcgg gtggaaagcg tgatgcgctt ttcccacttg acgtcctgta
     2941 aaccgtggtt gatggcattc tccacgatgg gctggaggat gacacccggc aggacaatcc
     3001 gctcgaccgg cccttcgatg gacttgacgt actggatttc gttggaaaac cgtacgttga
     3061 tgatatgcag atacgtctcc acctggctga tttcctcggc gagggttgac gtttgccccg
     3121 tatgtttgac gctgtagcgg aagaa
//
LOCUS       DAOMGY010000116         3142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_33001_length_3142_cov_3.646582, whole genome shotgun sequence.
ACCESSION   DAOMGY010000116 DAOMGY010000000
VERSION     DAOMGY010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33001_length_3142_cov_3.646582"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            349..843
                     /gene="nuoE"
                     /locus_tag="PLR57_02610"
     CDS             349..843
                     /gene="nuoE"
                     /locus_tag="PLR57_02610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HQQ40375.1"
                     /translation="MAKQKTAVPFRGTQEQEKQLRKIIADHKDVPGALMPVLQQAQEV
                     YGYLPIEVQNIIAEGLDISLEEVYQVVTFYGQFSLNPTGKHRVAVCLGTACYVKGSQN
                     ILEELEKQLGVKAGGTTPDGVFTLEATRCLGCCGLAPVIVVDDEVYGRLVPADIKGIL
                     AKYN"
     gene            892..1236
                     /locus_tag="PLR57_02615"
     CDS             892..1236
                     /locus_tag="PLR57_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HQQ40376.1"
                     /translation="MKLRQIADLLGAEVLCGEQQLDEEVHSACGSDMMSDVLAYVKDQ
                     AVLITGLVNPQVVRTAMMMDMRCVVFVRGKRPTQEMVELANENHIAVLVSGHRMYEAC
                     GKLYSGGLGCEA"
     gene            1241..1663
                     /locus_tag="PLR57_02620"
     CDS             1241..1663
                     /locus_tag="PLR57_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HQQ40377.1"
                     /translation="MAETLRFHYDVDGDNFTSAGAASVEVKRKLRQLGFPAETIRRVS
                     IAMYEGEINMVVHARGGTADVYVEPDSISIVLADQGPGIPDVELAMQEGYSTAPDNIR
                     ALGFGAGMGLPNMQRYTDEMKIETEVGKGTTVSMKVYV"
     gene            1776..3101
                     /locus_tag="PLR57_02625"
     CDS             1776..3101
                     /locus_tag="PLR57_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HQQ40378.1"
                     /translation="MDQYKHSVSLDIEKCKGCTNCLKRCPTEAIRIRDGHAVINSDVC
                     IDCGECIRRCPYQAKKANFDKFEDVDPKKYRIALPAPSFYGQFTELDDVDYVLQGLLD
                     IGFNEVFEVARAAEIVTEYTRRYMKSEDVRYPVINSACPAVVRLISLRFPYLCDHVIP
                     MMPPIEVAGRMAREEALEKHPELKPEDIAVVFISPCPAKASYVKNGFMEQKSNVDYVV
                     SMSDIYFKLIGVMKKSKLPQTSSQSGMIGMSWASTGGEASALFNDKYLAADGIENVIR
                     VLDEIETGHFPMLQFVELNACPGGCVGGVATVENPYIARVRMRALRRYLPVSLNRLSK
                     DDPEYLPKDMLFKHELEYRPVGKFDEDRALAMQKMSEIEALAETLPALDCGSCGAPTC
                     RAFAEDVVLGGRSVDECIVYMRERIQKLAAEQEAEQTKNDESGSEGEAK"
BASE COUNT          734 a          868 c          935 g          605 t
ORIGIN      
        1 cgagaattcg cataagttcg aggggtctcc tttcgatttc ttccgcgttt ctcccgacgc
       61 gcagtgtgcg tgcccatcgg cggaagttgt cattcatggg tattcaaacg ggaaaagcca
      121 tgttccgctc cgaaaacgtt tccatagagc gcagaacgca tctctattat aggcgcgcgg
      181 ataaactctg tcaaaggtgt gaataaatac atcaaaatcg ataaaagacg acggatttcg
      241 gcgttaaaat cgattttcag ctttcctaaa taatattgct atggtataat tcagcttggc
      301 ttataccgga caaagaccgg tttttgaatc acaccgagga ggaagattat ggcaaagcag
      361 aaaactgcgg taccgttccg cgggacgcag gaacaggaaa aacagctcag aaagatcatt
      421 gccgatcata aggacgtgcc gggcgcgctg atgccggtgc tgcagcaggc gcaggaagtc
      481 tacggctatc tgcccatcga agtgcagaac atcattgccg aagggctgga catttccctt
      541 gaagaagtgt accaggtcgt cacgttttac gggcaatttt cgctgaaccc caccggcaag
      601 caccgcgtcg cggtctgcct tggaacggcc tgctatgtca aaggctcgca gaacatcctc
      661 gaagaactcg aaaaacagct cggcgtcaaa gccggcggca ccacgccgga cggcgtgttc
      721 acgctggaag cgacccgctg cctcggctgc tgcggcctgg ctccggttat cgttgtggac
      781 gacgaagtct acggccgtct ggtccccgcg gacatcaaag ggattttggc caagtataac
      841 taagcgaata ccccaacacc aagctagact atccgaaggg atgaatgccg gatgaaacta
      901 cgtcaaatcg ccgatctgct cggcgccgag gtgctctgcg gagagcagca gctcgacgaa
      961 gaagtgcaca gcgcctgcgg cagcgacatg atgagcgacg tgcttgccta tgtcaaagat
     1021 caggctgtgc tgattacggg tcttgttaac ccgcaggttg tacgcaccgc catgatgatg
     1081 gatatgcgct gcgtcgtgtt tgttcgcggt aagcgcccca cccaggagat ggtcgagctt
     1141 gccaacgaga accacatcgc ggtgctggtc agcggtcacc gcatgtatga agcctgcggc
     1201 aagctctatt ccggcggcct cggctgcgag gcctgacgcg atggcggaaa cgcttcggtt
     1261 tcattacgat gtggacggcg ataacttcac ctccgcgggc gccgcgtccg tcgaggtaaa
     1321 gcgtaagctg cgccagctcg gttttcccgc cgagacgatt cgcagggtat cgattgcgat
     1381 gtacgagggc gaaatcaaca tggttgtgca cgcgcgcggc ggcacggcgg atgtttacgt
     1441 tgagccggac agcatttcga ttgttcttgc ggatcagggc cctggcatcc cggatgtgga
     1501 gctcgccatg caggaagggt attccaccgc gccggacaat atccgcgcgc tgggtttcgg
     1561 cgccggtatg ggactgccga acatgcagcg ctacaccgac gaaatgaaga tagaaacgga
     1621 agtcggcaag ggaacaacag tctcgatgaa ggtctacgta taactacgcc gctgacgcag
     1681 ggcaaacgga aatcctgtcg aagcgccgcc cggaacaatc gcgctgaaaa gcggcggtct
     1741 cccggcggaa cggcggaaag gcaagtctac cggccatgga tcagtacaag cattcggttt
     1801 cgcttgacat cgaaaaatgc aaaggctgca ccaactgcct gaaacggtgc ccgaccgaag
     1861 ccatccgcat tcgcgatggc cacgcggtga tcaattcgga tgtctgcatc gactgcggcg
     1921 agtgcatccg ccggtgcccc tatcaagcca aaaaggcgaa ctttgataaa tttgaggacg
     1981 tcgatcccaa gaagtaccgc atcgctctgc ccgcgccttc gttttatggg cagtttacgg
     2041 aactggacga cgtggactac gtgctgcagg gcctgctcga catcggcttc aacgaggtat
     2101 tcgaggtagc tcgtgctgcg gagatcgtaa cggaatacac ccgccgctat atgaagagcg
     2161 aggacgtccg ttaccccgtg atcaactccg cctgcccggc agtggtgcgg ctcatctcgc
     2221 tgcggtttcc gtatctttgc gaccacgtca tccccatgat gcctcccatc gaggtggcgg
     2281 ggcgtatggc gcgcgaggaa gcgctggaaa aacacccgga actcaagccg gaggacattg
     2341 cagtcgtctt tatctctccc tgcccggcaa aagccagcta tgtcaagaac ggctttatgg
     2401 agcagaagag caacgtcgat tacgtggtct ccatgagcga tatctatttc aagctcatcg
     2461 gcgttatgaa aaagagcaag ctgccgcaga cttcgagtca aagcggcatg atcggcatga
     2521 gctgggcgag cacaggcggg gaagccagcg cgctgttcaa cgataaatac ctggccgcgg
     2581 acggcataga aaacgtcatc cgcgtgctgg acgaaatcga aaccggacat ttccccatgc
     2641 tccagtttgt ggagctcaac gcctgccccg gcggctgcgt cggcggggtg gccacggtgg
     2701 aaaacccgta cattgcgcgt gtacgcatgc gggcgctcag gagatacctg ccggtttcgc
     2761 tcaaccgcct cagtaaggac gacccggaat accttcctaa agatatgctc tttaagcatg
     2821 agctggagta ccgccccgtc ggcaagttcg acgaggaccg cgcgctcgcc atgcagaaga
     2881 tgagcgaaat cgaagcgctg gcggaaacgc tgcccgcgct cgactgcggg agctgcggcg
     2941 cgccgacctg ccgcgcgttt gccgaggacg tcgttcttgg cggacggagc gtggacgagt
     3001 gcatcgtcta catgcgcgag cggattcaaa agctcgccgc cgaacaggaa gccgagcaga
     3061 cgaagaacga cgagagcggt tcggaagggg aagcgaaatg acggtagccg aactcatcca
     3121 aaagctgaac cttgagccgt tt
//
LOCUS       DAOMGY010000117         3099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_33653_length_3099_cov_3.618594, whole genome shotgun sequence.
ACCESSION   DAOMGY010000117 DAOMGY010000000
VERSION     DAOMGY010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33653_length_3099_cov_3.618594"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..942)
                     /gene="murB"
                     /locus_tag="PLR57_02630"
     CDS             complement(40..942)
                     /gene="murB"
                     /locus_tag="PLR57_02630"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQQ40379.1"
                     /translation="MENTLDRVYAAIKGLNVKCNIPLSTLTSFRIGGPAAMVLRPRSA
                     EDIRRTIQACSQFGCPYCVLGNGTNVLAPDSGYPGVIIRIDTPMTTPLFVDTTATSPA
                     GTALSVLARDSVANGLMGMETLSGIPGTVGGAVAMNAGAYGAEIRHLIKSVRILKNGR
                     VLDVETKKSDFGYRESKYAAPGVIVLSATFQLEHDDGQAKQRMLETSRQRKEKQPLSY
                     PSAGSVFKRPNGRFAGALIEGCGLKGFSVGGAQVSPLHAGFIVNTGNATERDVLELIA
                     IIQQRVFDDSGVRLEREVKLLSEL"
     gene            complement(1070..1555)
                     /locus_tag="PLR57_02635"
     CDS             complement(1070..1555)
                     /locus_tag="PLR57_02635"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HQQ40380.1"
                     /translation="METVFHTIPPVFSEHSEILILGTMPSPKSREAAFYYAHPQNRFW
                     PALALVYGAEPPKTTGQKVALLLEHNLALWDVLKSCEITGASDASIRNPVANDISALL
                     KQTPIRRVLCTGSTSARLYRTLVEPLTHLPCTALPSPSAANARTSLEALVCAYKAALL
                     G"
     gene            1701..>3099
                     /locus_tag="PLR57_02640"
     CDS             1701..>3099
                     /locus_tag="PLR57_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40381.1"
                     /translation="MSTIKRFLSYYKPHIGLLVLDLSCAFTVAAIDVAYPMLTQYILR
                     TLLPQMAIDSALVRVFVLLLIGALAAYIVRAVLLYVINYWGHRLGILMEADIRKDIFS
                     HIQQLSFSFFDKVRTGKLMSRVTTDLFDITELAHHGPEDVLISITTLIGAFIAMAVLE
                     WRLALALLCIVPVGVFYVMRIRIKMRKASLTVKERVAEINADIESSISGARVAKAFTN
                     EEHELKKFDKGNKSFVHAKDRFYMYMAFFNSGMEFFVGLFNLVVLGVGGYLIYKSSLD
                     PILLVTFTLYIATFVQPLKRLASFTELYILGMAGFSRFCEIMDIEPDIKDRPNAKPLQ
                     NVKGDICFTDVSFAYESGRSVLSHINLDIPAGKTLALVGPSGGGKTTLCHLIPRFYEL
                     RSGSITIDGTDIRDITLQSLRQNVGIVQQDVFLFASSVLENIRYGRIDATDAEVVEAA
                     KRAHIHDEIQLFPNGY"
BASE COUNT          650 a          844 c          881 g          724 t
ORIGIN      
        1 gaacccttct atatagcttc aatatgcagt atgaatcgtt tacagctcgg agagcagttt
       61 gacttcgcgc tccagacgta cgccggaatc gtcgaacacg cgctgctgga tgatcgcaat
      121 caattcaagc acgtcccgct cggtagcgtt gccggtgttg acgataaacc ctgcgtgcag
      181 cgggctgacc tgcgcgccac ccacggagaa tcctttgagc ccgcagcctt cgatcagcgc
      241 gcccgcgaac cgcccgttcg ggcgcttgaa cacgctgcct gcgcttgggt agctgagcgg
      301 ctgcttttcc ttgcgctgtc gcgaggtttc cagcatgcgc tgcttggcct ggccgtcgtc
      361 gtgctccaac tggaacgtag ccgaaagcac gatcacgccg ggcgcggcgt atttgctctc
      421 gcgataaccg aagtcggatt ttttcgtttc aacgtccagc acacgcccgt ttttgaggat
      481 gcggacgctt ttgatcaaat gccgtatctc cgcgccgtat gcgcccgcgt tcatggcaac
      541 cgcgccgccg accgtgcccg gaatgccgga gagggtttcc atgcccatca ggccgttggc
      601 aaccgaatcc cgcgcgagaa cggagagtgc agtacccgcc gggcttgtgg cggtggtgtc
      661 cacaaacaaa ggcgtagtca tcggcgtgtc gatgcggatg atgaccccgg gatagcccga
      721 gtccggcgcg agcacgttgg tgccgttgcc caatacgcag tagggacagc cgaactggct
      781 gcaagcctga atggtgcggc gaatatcttc tgcgctgcgc gggcgcagca ccatggccgc
      841 gggcccgccg atgcggaagc tcgtgagggt ggaaagcgga atattacatt ttacgtttaa
      901 gccctttatg gcggcataaa cgcggtcgag cgtattctcc atacaagcta tgataacaaa
      961 ttagacatat tttaacaacg gatttgagcg caaaaaatcg atttcgggac gaaaatttat
     1021 aaatttattc gatcgtttat gcaaatatga acaaattgcg acgttgtgtt catcccagca
     1081 aagcggcttt gtaggcgcaa acaagcgctt ccagagacgt cctcgcgttc gcggcactgg
     1141 gcgagggaag cgccgtgcag gggagatgcg tcagcggctc gacgagcgtt cggtaaagcc
     1201 gcgcgctggt ggaacccgtg cagagtacgc gccggatggg agtctgcttc aggagcgcgg
     1261 atatatcgtt ggcaaccggg tttcttatgc tggcgtcgct tgcgcctgtg atttcgcagc
     1321 ttttcagcac gtcccagagc gcaaggttgt gctcaagcaa aagcgcaacc ttctgtccgg
     1381 ttgtttttgg tggttccgcg ccgtaaacca gcgcgagcgc cggccagaag cggttctgcg
     1441 gatgcgcgta atagaatgcc gcttcgcgcg atttcggcga gggcattgtg ccgagaatta
     1501 agatttcgct gtgttcagag aacacgggcg ggatggtatg aaacacggtt tccatagcaa
     1561 tagcctactc cgttttgccc gcgcggtcaa acggttcact tgaggcgcgg aacggttgaa
     1621 gctgcatgaa acgcaaaata taaaaatcga accgctcatg cgagattgat tcaaccggcg
     1681 ggttctgtta agatgaacgc atgagtacga tcaaacgatt tctttcatat tataaaccgc
     1741 atatcggttt gctggttctc gatttaagct gtgcgtttac cgttgccgct atcgacgttg
     1801 cttaccctat gcttacgcag tatatcctgc gcacgctcct gccgcaaatg gcaatcgact
     1861 ccgcgctggt gcgtgtgttt gtgcttctgc tcatcggcgc gctggcggcg tatatcgtgc
     1921 gcgcggtgct gttgtatgtc atcaactact gggggcaccg ccttggcatc ctgatggaag
     1981 cggatatccg aaaggacatc ttttcgcata ttcagcagct ttccttcagt tttttcgata
     2041 aggtacgcac cggcaagctc atgagccggg taacgacgga cttgttcgac atcaccgaac
     2101 ttgcgcacca cggccccgag gacgttttga tttcgattac aacgctcatc ggcgcgttca
     2161 tcgccatggc ggtgctcgag tggcggctgg cgctggcgct gttgtgcatc gtgcccgtcg
     2221 gcgtgtttta cgtgatgcga attcgcatca aaatgcgcaa ggcttcgctg acggtcaaag
     2281 agcgcgttgc cgaaatcaac gccgatatag aatcttccat ctccggcgcg cgcgtagcca
     2341 aagcctttac caacgaagaa cacgaactga aaaagttcga caagggcaac aagagctttg
     2401 tgcatgccaa ggaccgcttc tatatgtata tggcgttctt caacagcggt atggagttct
     2461 ttgtgggctt attcaacctc gtcgtgcttg gcgtgggcgg atacctgatt tacaagagca
     2521 gtctcgatcc cattctgctc gttaccttta cgctttatat cgcaacgttt gttcagccgc
     2581 tcaagcgcct tgccagcttt acggaactct atatcctcgg catggccggc ttttcgcgtt
     2641 tctgcgaaat tatggatatc gagccggata tcaaagaccg ccccaacgca aagccgctgc
     2701 aaaatgtgaa gggcgacatc tgctttacgg acgtttcgtt tgcttatgaa agcggaagga
     2761 gcgtgctttc gcatatcaat ctggatattc ccgccggtaa aacgcttgcg ctcgtcggtc
     2821 cttccggcgg cggcaaaacg acgctctgcc atttgattcc gcgtttctac gaactgcgca
     2881 gcgggtcgat taccatcgac ggaacggaca ttcgggacat caccttgcag tcgctccgcc
     2941 agaacgttgg catcgttcag caggacgtgt tcctctttgc cagcagcgtg ctcgaaaaca
     3001 ttcgctacgg gcgcattgac gcgaccgacg cggaggtggt ggaggcggca aagcgcgcgc
     3061 atattcacga cgaaattcag ctgttcccca acgggtacg
//
LOCUS       DAOMGY010000118         3092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_33771_length_3092_cov_3.620678, whole genome shotgun sequence.
ACCESSION   DAOMGY010000118 DAOMGY010000000
VERSION     DAOMGY010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33771_length_3092_cov_3.620678"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..319)
                     /locus_tag="PLR57_02645"
     tRNA            complement(243..319)
                     /locus_tag="PLR57_02645"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(283..285),aa:Val,seq:cac)
     gene            623..1984
                     /gene="murC"
                     /locus_tag="PLR57_02650"
     CDS             623..1984
                     /gene="murC"
                     /locus_tag="PLR57_02650"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HQQ40382.1"
                     /translation="MAHIDEAKRIHLIGIGGCSMNGLALILQSQGHTVTGSDRERTAF
                     MDALDKDGIRYSVGHTGEYVGGADLVIYSAAIKPDNPERVLAREKGIPELERSVALGQ
                     ISERYREVVAVAGCHGKTTITSMLALINDLAKTDATIHVGGFVEFLGGGVRVGSQDLL
                     VTEACEYVESFLTLKPTVALINNIDDDHLDYYKDIDAIVEAFEKFLALLPEDGVFVAC
                     ADDPRVMKLAKQSDLNVVTYGLTGGDYTAKDVEFDAEGNAGFTVLFHGEPLGKVQLSV
                     PGMHNVVNALGSIAVADRLKVPFAVSAKALNQFRNTKRRFEYYGERNGVKVFHDYGHH
                     PNEIRATLDAAKRVPHNRLICVFQCNSYTRACTLFCEHVVCFRDADMVLVPDIYPGRE
                     KDTGIVHARDMVAAINRESDNAVYLPTFEEIRLYLDEHAEEGDLVVTVGSGDVYKQTR
                     KLL"
     gene            2066..2908
                     /locus_tag="PLR57_02655"
     CDS             2066..2908
                     /locus_tag="PLR57_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQQ40383.1"
                     /translation="MPSFIQILKEYLPDKSVMDVRPPFDDDLSQPLFVKGGTVGCLVL
                     HGIGGTPANVRVVADALVQKGYTVLSPTLPGHGETVRAQNRSTGREWLACARESYQRL
                     RNEGCEQIYALGLSLGGILCGLLAEEEWLDGLALICAPIKMTRYLRTARAMSFLLPVV
                     RYPETRDGSTAWGDNLYKQMYGGFSTTKLVDLGRLSRRLKRNLARIVCPTLVVSAKFD
                     DKVDPVSVEIFRKRAVNVESAEYVLFENSPHGCTYGPEKEQVAEKCAEFVSGLVDKKA
                     SASV"
BASE COUNT          652 a          878 c          931 g          631 t
ORIGIN      
        1 cttgcatgaa cttctttatt tccctaaagt gttgcacttg atagtataat tcaccgaact
       61 agaaaaaagt tatccgcacg atcttgggac aactagctgc aaaactaatt agaaatttaa
      121 atagtcttta aatgggaagc catactttta caaacactag aaggtagctc taagtgggtt
      181 attaagtggg tttttggcaa tagaaaagag ccggaatcgc tagattctat cggctctctt
      241 tttggtgggg atggaggggc tcgaaccccc gacctcttcg atgtgaacga agcgctctga
      301 ccaactgtgc tacatcccca agcaaaggtt attttaccgc atcagtcgga gaatggcaag
      361 cctttctttt catttcagca tgcgttttcc ggtgaattaa ggcacatagg ttgggggtac
      421 gccaagcttt cttttttcgt tttcgggtgc gttttgacgg catagcgttc gacgactggc
      481 cggatgtgaa tcccgctttc agtgaaaaaa acctgattcg cgggggatgg cttggtcgtc
      541 aaagttgcga aacgcatacg ctgaggcgta taatatacta gtttacagaa cagcgattta
      601 ccgcataggg agttaaagat tcatggcaca tatcgacgaa gcaaagcgca tccacctgat
      661 cggcatcggc gggtgcagca tgaacgggct cgcgctcatc ctgcagtcgc aggggcatac
      721 cgtgaccggc tccgaccgcg agcgcaccgc gtttatggac gcgctcgaca aggacggcat
      781 ccgctacagc gtaggccaca cgggcgaata cgtcgggggg gcggatctcg tgatctactc
      841 cgccgcgatc aagcccgaca atcccgagcg cgtgcttgcg cgcgaaaagg gcattcccga
      901 actggagcgc agcgtggcgc tggggcagat cagcgagcgc taccgcgaag tggtcgccgt
      961 tgcgggatgc cacggcaaaa cgaccatcac ctccatgctc gcgctcatca acgatctggc
     1021 aaagacagac gcgaccatcc acgtaggcgg gtttgtggag ttcctgggcg gcggcgtgcg
     1081 cgtgggcagt caggacctgc tcgtcacgga agcctgcgag tatgtggaaa gctttctaac
     1141 gctcaaaccg accgtcgcgc tgatcaacaa catcgacgac gaccatctgg attactataa
     1201 ggatatcgac gccattgtcg aggcgtttga aaagtttctg gcgctcctgc cggaggacgg
     1261 cgtgtttgtt gcctgcgcgg acgacccccg cgtgatgaag cttgccaaac agtccgatct
     1321 caacgtcgtc acctacgggc tcaccggcgg cgattacacg gcaaaagacg tcgaattcga
     1381 cgctgagggc aacgcgggct ttaccgtgct gttccacggc gaaccgctgg gcaaggtgca
     1441 gctctccgtg ccgggcatgc ataacgttgt caacgcgctg ggctcgattg cggttgcgga
     1501 ccggctgaag gttccgttcg cggtttccgc caaggcgctg aaccagttcc gcaacaccaa
     1561 gcgaagattc gagtactacg gcgagcgcaa cggcgtgaag gtgtttcatg attacggcca
     1621 ccaccccaac gaaatccgcg caacgctgga cgccgccaag cgcgtgcccc acaaccgcct
     1681 gatctgcgtg ttccagtgca acagctacac ccgcgcgtgc acgctctttt gcgagcatgt
     1741 ggtgtgcttt cgggacgcgg acatggtgct cgtgccggat atctaccccg ggcgcgaaaa
     1801 ggacaccggc atcgtccacg cgcgggacat ggtagccgcc atcaaccgcg agagcgacaa
     1861 cgccgtgtat ctgccgacgt tcgaggagat tcgcctgtat ctggacgagc acgccgagga
     1921 aggcgacctc gttgtcaccg tcggctccgg ggacgtgtat aagcagacgc gaaaactcct
     1981 ctagaaagcg gctttcgttc gattccaccg cccgcgttgt tgggcgcgtg agctacccgg
     2041 ccaacgttcg gaaaggagcg gttgcatgcc gtcgttcatt cagattttga aggaatacct
     2101 gccggacaag agcgttatgg acgtgcgccc gccgtttgac gacgacctgt cccagccgct
     2161 gttcgtcaag ggcggcaccg tcggctgcct cgtgctgcac ggcatcggcg gcacgcccgc
     2221 gaacgtccgc gtcgtggcgg atgcgctcgt tcaaaaaggc tataccgtcc tttcgcccac
     2281 gctgccgggg cacggcgaaa ccgtccgcgc gcagaaccgc tccactggta gggaatggct
     2341 tgcctgcgcg agggaatcct accagcgcct cagaaacgag gggtgcgagc aaatttacgc
     2401 gctcgggctt tcgctcggcg gcatcctctg cgggctgctg gcggaggaag agtggctgga
     2461 cggccttgcg ctcatctgcg cgccgattaa gatgacgcgt tacctccgca ccgcgcgcgc
     2521 catgagcttc cttctgcccg tggtgcggta tcccgaaacg cgggacggtt caaccgcctg
     2581 gggcgacaac ctctataagc agatgtacgg cgggttttcc accacgaagc tcgtcgatct
     2641 cggccgcctg agccgcaggc tcaaacgcaa cctcgcgcgg atcgtctgcc cgacgctcgt
     2701 cgtttccgcc aagtttgacg acaaggtcga tcccgtgagc gtggagattt tccgcaagcg
     2761 cgcggtcaac gttgagagcg cggagtatgt gctgttcgaa aactcgccgc atggctgcac
     2821 ctatggcccg gaaaaggagc aggtggcgga gaagtgcgcc gaattcgttt ccggtttggt
     2881 tgacaagaaa gcgtccgctt cggtataatt cctctcgtgc gccgcgcgaa atatagcgga
     2941 ggtttgcggc ggctcgcccg ttggttagga cggcgggaga gcggggaaaa gtgaataggg
     3001 taaaagtgtg tctccgtagc tcagtaggat agagcgttcg cctcctaagg cggaggacgt
     3061 acactcgcct tgagccactc cccaaagtcc gt
//
LOCUS       DAOMGY010000119         3082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_33926_length_3082_cov_2.548398, whole genome shotgun sequence.
ACCESSION   DAOMGY010000119 DAOMGY010000000
VERSION     DAOMGY010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33926_length_3082_cov_2.548398"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLR57_02660"
     CDS             <1..235
                     /locus_tag="PLR57_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HQQ40384.1"
                     /translation="GHIKERIRALGYALDDEEMALGVRCLAVPVYDYRNSVRYSIGVS
                     GPKANISSATMETYIKRMVEATRKLSAAIGSTR"
     gene            339..1544
                     /locus_tag="PLR57_02665"
     CDS             339..1544
                     /locus_tag="PLR57_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQQ40385.1"
                     /translation="MEKLKFVLIGCGRIATLHVAGYKDREDAELWGVYDKNPQTAKRF
                     AAEHGIPKVYDSYEAVLADPEVTGVEILVPHHLHCELTIAACKAKKHVSVQKPMAMSL
                     AECDKMIAAAKENGVLLKVFENFVHYPPYLLLKKMIAEGEIGEIVGIRYKMCNASLFS
                     DNIPGAGARAALAGVKDAKDCERTGWKVDPSSWLWRLNDTLCGGGPAVFDDGYHKFSL
                     FVDLLGEVEKVCAWIDETPALPGLMQDVPSVIMWKYAGKKVYGVWDTTSAPELYVKGS
                     YYTCDERMEVTGTRGVLWLTRCTAEMLESVAPVVLYKDGKLTEFWDEPSDWQDAFIHS
                     THDFVDAVKEGREPILSGERGREVLAFALAAIDSSACKKEVYLDQYQDKPTGKRLGFF
                     AAMRRKKHG"
     gene            1537..>3082
                     /locus_tag="PLR57_02670"
     CDS             1537..>3082
                     /locus_tag="PLR57_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ40386.1"
                     /translation="MAKEPGKLKMKLAYAAGDIYGGGAFLIFSMLYMNFLVLVEGLPV
                     VATSLIVFIGKIWDAVTDPLMGRISDRTRSRFGRRRLYFLLGIIPVFASFVMMFYSFG
                     IVSTLSKIIYYTCAYMFFGTAFTIVMVPYNAILSDMTSDYNERTSFTTIRMLFSGGAS
                     LIAAVLPGTLIKLFGGSANGPAQKPGYLIMALVMATAFGLCWLFAFLGTREKENLPPP
                     EKITVRDWLSPFANKAYRNYLGIFLSFQVAVDLVLALFIFYIDIVVLKYTSYTLVMGV
                     LLVFSLVFMAVMGAVAQKKGKAFPLYIGMPIWIVTMAGFLFANSSTSVYILCALAVLI
                     AVGSASGNLSTWSMLTDIFDIDEIRTSKRREGIYSGLTTFLRKFASGVAILVLGFGLK
                     ALGFDQNQYNLLKASGASFDPATYAQSSLVSGIKWMFVLIPVLLLSITLFFAIKNKVN
                     KRRFDAVLKGIDSFQKTGSLAELNEQEKEDVRLVTGKRKISSGASKAYTVGNTKPRNI
                     RGFVCFM"
BASE COUNT          655 a          897 c          882 g          648 t
ORIGIN      
        1 cggccacatc aaggagcgca tccgcgcgct tgggtacgcc ctggacgacg aggagatggc
       61 gctgggcgtc cgctgcctcg cggtgccggt atacgactac cgtaacagtg ttcgctacag
      121 catcggcgtt tccggcccga aagccaacat cagttccgca accatggaaa cctacatcaa
      181 gcgcatggtg gaagcgaccc gaaagctcag cgccgccatc ggcagcacgc gctgaggcaa
      241 ctcctttcca gcaacttccg cccggtatca tttcgtttta cccgccaggc aaaaaccttt
      301 catacaacag aatcaattct cgaaaagagg aagaaccgat ggaaaaactg aaatttgtcc
      361 tgattggctg cgggcgtatc gcaacacttc acgtcgcggg gtataaggac cgcgaggacg
      421 cggagctctg gggcgtgtac gataaaaacc cgcagacggc caagcgcttt gccgccgaac
      481 acggcatccc gaaagtgtac gattcctacg aagcggtgct cgccgatccg gaagtgaccg
      541 gcgtggaaat cctcgtaccg caccatctgc actgcgaact gacaattgcc gcctgcaagg
      601 caaaaaaaca cgtttccgtg caaaagccca tggccatgag cctggccgaa tgcgacaaaa
      661 tgatcgcagc ggcaaaggaa aacggcgtgc tgctcaaggt gtttgaaaac tttgtgcact
      721 acccgccgta cctgctgctc aaaaagatga tcgcggaagg cgaaatcggg gaaatcgtcg
      781 gcatccgcta taaaatgtgc aacgcctcgc tcttttcgga caacatcccc ggcgcgggcg
      841 cgcgggctgc tcttgcgggc gtcaaggacg ccaaagactg cgagcgcacg ggttggaagg
      901 tcgatccttc gagctggctc tggcggctca acgacacgct ctgcggcggc ggccccgccg
      961 tgttcgacga cggctaccat aaattctcgc tgtttgtgga tctgctggga gaagtcgaaa
     1021 aggtctgcgc ctggatcgac gaaacgcccg ctctgccggg gctcatgcag gatgttccgt
     1081 ctgtgattat gtggaaatac gcgggtaaaa aggtctacgg cgtgtgggac accacctccg
     1141 cgccggagct ctacgtcaag ggcagctact acacctgcga tgagcgcatg gaggtcaccg
     1201 gcacgcgcgg cgtgctgtgg ctgacccgct gcacggcgga gatgctcgaa agcgtcgccc
     1261 ccgtcgtgct ctacaaggac ggcaagctca cggagttctg ggacgagccc agcgactggc
     1321 aggacgcgtt tatccactcg acccatgact ttgtcgacgc cgtcaaggaa ggccgggagc
     1381 cgatcttatc cggcgaacgc gggcgcgagg tgcttgcgtt tgcgctggcg gccatcgact
     1441 cttccgcctg caaaaaggaa gtgtacctcg accaatatca ggacaagccg acgggcaagc
     1501 gtttgggctt tttcgcggcg atgaggagga aaaaacatgg ctaaggaacc cggcaaactc
     1561 aaaatgaagc ttgcctatgc cgcgggcgat atctacggcg gcggcgcgtt tctgatcttc
     1621 agcatgctgt atatgaactt cctcgttctg gtcgagggac tgccggtcgt tgccacgagt
     1681 ttgatcgtgt tcatcggcaa aatctgggac gcggtgaccg accctcttat ggggcgcatc
     1741 tccgaccgca cgcgttcgcg cttcggccgg aggcggctgt atttcctgct cggcatcatt
     1801 ccggtattcg cgtcgtttgt catgatgttc tattcgttcg gcatcgtgag cacgctctcc
     1861 aagatcatct actacacgtg tgcctacatg ttcttcggaa cggcgttcac catcgtaatg
     1921 gtgccctaca acgccatcct ttcggacatg acaagcgact ataacgagcg cacgagcttt
     1981 acgaccattc gtatgctctt ctccggcggc gcttcgctga ttgcggcagt tctgccggga
     2041 acgctgatca agctctttgg aggcagcgcc aacggccctg cgcagaagcc cggctatctg
     2101 atcatggcgc tcgtaatggc tacggcattc ggactctgct ggctgtttgc cttcctcggc
     2161 acgcgcgaaa aggaaaatct gcccccgccg gaaaagatca ccgttcgcga ctggctcagc
     2221 ccctttgcca ataaggcata ccgcaactac ctcggcatct ttctctcgtt tcaggtcgcg
     2281 gtggatctcg tgctggcgct gttcatcttc tatatcgaca tcgtcgtgct caagtacacc
     2341 agctatacgc tggtcatggg cgtgctgctt gtgttctcgc tggtgtttat ggcggtgatg
     2401 ggcgcggtgg cgcagaaaaa gggcaaggcg ttcccgctct atatcggcat gccgatctgg
     2461 attgtcacaa tggccggatt cctttttgcc aacagcagca cttccgtcta catcctctgc
     2521 gcgctcgcgg tgctcatcgc ggtcggctcc gccagcggca acctctccac ctggtcgatg
     2581 ctgacggata tcttcgatat cgatgaaatc cgcacctcca agcggcgtga gggcatctac
     2641 agcgggttaa cgacattcct gcgcaagttc gccagcggcg tggccatact ggtgctcggt
     2701 ttcggcctaa aagcgctggg gttcgaccaa aaccagtaca atcttttaaa ggcttccggc
     2761 gcaagtttcg atccagcgac gtacgcgcag agttcgctcg tctccggcat caagtggatg
     2821 ttcgtgctca tccccgtact tctgttgagc attacgctgt ttttcgcaat caaaaacaaa
     2881 gtcaacaaac ggcggttcga tgcggtgctc aagggcatcg actcgttcca gaagaccggg
     2941 tcgctcgccg agctgaacga gcaggaaaaa gaagacgtcc gcctcgtcac cggaaaacgg
     3001 aagatcagct ctggggcaag taaggcgtat actgtaggta acacaaaacc gcggaatatc
     3061 cgcggttttg tttgttttat gc
//
LOCUS       DAOMGY010000120         3078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_34002_length_3078_cov_3.429375, whole genome shotgun sequence.
ACCESSION   DAOMGY010000120 DAOMGY010000000
VERSION     DAOMGY010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34002_length_3078_cov_3.429375"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLR57_02675"
     CDS             complement(<1..287)
                     /locus_tag="PLR57_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40387.1"
                     /translation="MLGNVFKKLFLLLIVTALLVGTALGVWCAPRTDLFFWESATPTP
                     TQTAVLATTTSPEPEATAEPTPTPVAEPATTEEWIDRYIEGVIKADDLEHI"
     gene            569..2257
                     /locus_tag="PLR57_02680"
     CDS             569..2257
                     /locus_tag="PLR57_02680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HQQ40388.1"
                     /translation="MEKFSEIVYKRPDMAALKKAMKQQLELLKKAKTYEEARAAYLAS
                     QEITAHAETSYVVASVRNTLDTTDKFYDGEMQFFNRALAMLMPISKAFIDAILKTPFR
                     AEFEAEFGKQLFTLAEMAQKSQSKKIIPELIAQGTTENNYKKTAAACKTTFRGEECNF
                     YGLLKHMESPDREERKEAYLAWAKLYEGASEQLDKQFDKLIKLRVRMAKKLGLPGGFT
                     ELGYLNMHRANYGPAEVEQFRAQVKAVIVPAVAKLREEQAKRLGVEKLKYYDETCIYP
                     DGNADPVGGEADLVAVAQRMYREISPETGEFFDFLFEYGLFDLATRPGKHLGGYCTYL
                     ADYKAPFIFSNFNGTAADVNVLTHEAGHAFAGYTAMRSQPLMDYMSSTSEVNEIHSMT
                     MEHFAYPYMKNFFGEENVAKAKYAHLSGALSTIPYLVSVDEFQHRVYAKPEMTAKERR
                     AVWHEIEQTYMPWRDYDGVPFLEEGGFWMQKQHIFLYPFYYVDYALAQVCAFEFYGRM
                     KKDHSDAWESYLALCRAGGSKGYFELLQLAGLSNPFVEGSVEKAVRHVIEELNV"
     gene            2571..>3078
                     /locus_tag="PLR57_02685"
     CDS             2571..>3078
                     /locus_tag="PLR57_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HQQ40389.1"
                     /translation="MTKVMKIILITLLVVVLAGAGLIWYVLGSMRSGAVETIEKPTED
                     VSVTEQPEVITVISDEPTPSPTPMPIYAEESKEDSIVNILLVGTDSRSESELESSEGR
                     SDSMMLASLNKEKGTITLISFMRDSRVHRIGKSGRFTFYNRLNGAYSGGYGGGGPGEL
                     INTLNENFD"
BASE COUNT          763 a          881 c          817 g          617 t
ORIGIN      
        1 tgaatatgtt ccaggtcatc cgctttgatg accccttcga tatagcggtc gatccattcc
       61 tccgtcgtcg cgggttccgc aaccggagta ggcgtcggtt ccgccgttgc ttccggctca
      121 ggagaagtcg ttgttgccag cactgccgtc tgcgttggcg ttggagttgc gctctcccaa
      181 aagaaaaggt ctgttcgcgg ggcgcaccaa acgccgagcg ccgtgccgac cagcagcgcc
      241 gtcacgataa gcagcaggaa tagcttctta aaaacgttac ctaacaatcg gtaattctcc
      301 gtttcaaatc agcgcaacga aaaatgatcc cgccgccgcg cgtgatatgg atgtattata
      361 ccctgcataa acccgaattg caaacaccgc cgcacagccg ccggcagaac gctttttcca
      421 aatacacggc aattacgggt tgccaaccgc aagactgcat attacaatac agttaatgtg
      481 tttatgcagg cattcaggca gtcctgccgg gtttccctgt cggtttttca acaggaaaac
      541 acataattaa tgaaatcggg ggcgcatcat ggagaaattt tcggagatcg tatacaaacg
      601 gccggacatg gccgcgctga aaaaagcgat gaaacagcag ctcgaactgc tgaaaaaagc
      661 aaagacgtac gaagaagccc gcgcggcata ccttgcctcg caggagatta ccgcgcacgc
      721 ggaaacgagc tacgtcgtcg ccagcgtccg caacacgctg gacaccacgg acaagtttta
      781 cgacggcgaa atgcagtttt tcaaccgcgc gctcgccatg ctgatgccca tctccaaagc
      841 gtttatcgac gcaatactca aaacgccgtt ccgcgcggaa tttgaagccg aattcggcaa
      901 acagctcttt acgctcgcgg aaatggcgca gaagtcgcag agcaaaaaaa tcattccaga
      961 actgattgcg cagggcacga ccgaaaacaa ctataaaaag accgccgccg cctgcaaaac
     1021 gaccttccgc ggcgaagaat gcaactttta cggcctgctc aagcacatgg agagccccga
     1081 tcgcgaagag cgcaaggaag cgtatctggc ctgggcaaag ctctacgaag gcgcttccga
     1141 gcagctcgac aagcagttcg acaagctgat caagctccgt gtccgcatgg caaagaagct
     1201 cggccttccc ggcggcttta cggaactcgg ttatctcaac atgcaccgcg caaactacgg
     1261 tcccgcggaa gtggagcagt tccgcgcgca ggtcaaggcg gtcatcgttc ccgccgtggc
     1321 aaagctccgc gaagaacagg caaagcgcct cggcgtggaa aagctcaagt actacgacga
     1381 aacctgcatc tatccggacg gcaacgcgga tcccgtcggc ggcgaagcgg atctggtcgc
     1441 ggttgcgcag cgcatgtacc gcgagatctc gccggaaacg ggcgaattct tcgacttcct
     1501 ctttgaatat ggtttgttcg acctcgcaac ccgtcccggc aagcacctcg gcggctactg
     1561 cacgtacctt gccgactaca aagcgccgtt catattcagc aacttcaacg gcacggcggc
     1621 ggacgtcaac gtcctcaccc acgaagcggg tcacgcgttc gcgggctata ccgccatgcg
     1681 cagccagccg ctgatggact acatgagttc gaccagcgaa gtcaacgaaa tccactccat
     1741 gaccatggag catttcgcct atccgtacat gaagaacttt tttggcgaag aaaatgttgc
     1801 caaagccaag tatgcgcacc tgtccggcgc gctctcgacc attccgtatc tggtcagcgt
     1861 ggacgagttc cagcaccgcg tttacgccaa gccggagatg accgccaagg agcgccgcgc
     1921 tgtctggcac gaaatcgagc aaacatacat gccctggcgg gactacgacg gcgtgccgtt
     1981 cctcgaggaa ggcggcttct ggatgcagaa acagcatatc ttcctctacc cgttctatta
     2041 tgtggactat gcgctggcgc aggtctgcgc gtttgaattc tacggccgca tgaagaaaga
     2101 ccattccgac gcctgggaat cctatctcgc gctctgccgc gcgggcggga gcaaagggta
     2161 tttcgaactg ctgcagctcg cggggctttc caacccgttt gtcgaaggca gcgtggaaaa
     2221 agccgtgcgg cacgtcatcg aagagctgaa cgtataaatt ccgtcattac aaaaagtccg
     2281 cttcgaacaa tcgaagcgga cttttttatc attttaagcg ggataaacta aatatgattg
     2341 cggatgtgca ggatggacgg caggcgcgcg tcctgccagt tcgctgcaaa gtattccatg
     2401 acgtccccgt aaataccggc gaaagcagaa tatttcataa ttgccccagt tgtaaaaaag
     2461 ttgtgagttt tccggcattc tgcgaaatca aattgaaaca aaccgcccgt tatggtatca
     2521 tggtctcgct ttacagcgat cttgaccatc ggtttagaaa ggtacctatt atgaccaaag
     2581 tcatgaaaat tatactgatc accctgttgg tggtcgtact ggccggcgcg ggtttgattt
     2641 ggtacgtgct cggaagcatg cgttccggtg cggtggaaac gattgaaaaa cccaccgagg
     2701 acgtttccgt taccgaacag cccgaagtca tcaccgtcat ttcggacgag cccaccccgt
     2761 cccccacgcc gatgccgatc tatgcggaag agagcaagga ggacagcatc gtcaacattc
     2821 tgctcgtcgg cacggattcg cgcagcgaaa gtgagctgga aagctccgag gggcgttccg
     2881 actccatgat gctcgcttcc ctcaacaaag agaagggcac gattaccctg atttcattca
     2941 tgcgcgattc gcgcgtacac cgcatcggca agagcggcag gttcaccttc tataaccgtc
     3001 tgaacggcgc ttacagcggc ggttacggcg gcggcggccc cggagaactg atcaacaccc
     3061 tgaacgagaa cttcgatc
//
LOCUS       DAOMGY010000121         3068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_34151_length_3068_cov_2.840358, whole genome shotgun sequence.
ACCESSION   DAOMGY010000121 DAOMGY010000000
VERSION     DAOMGY010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34151_length_3068_cov_2.840358"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLR57_02690"
     CDS             complement(<1..255)
                     /locus_tag="PLR57_02690"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40390.1"
                     /translation="MQQACEVIVDIAHANVDRLFTYLLPEGLTLPAGSHVLVPFGNGN
                     RQLEGFVIRVMEASEMPTERDETQPSPVSLKPLLRVIEPYP"
     gene            complement(512..712)
                     /gene="rpoZ"
                     /locus_tag="PLR57_02695"
     CDS             complement(512..712)
                     /gene="rpoZ"
                     /locus_tag="PLR57_02695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQQ40391.1"
                     /translation="MMNLPLNELIEKAGCRYMLVTAVSQRARQLQSEPDKLDGRKPVS
                     VAVDELYRDKLNLEYPKEFYQD"
     gene            complement(709..1341)
                     /gene="gmk"
                     /locus_tag="PLR57_02700"
     CDS             complement(709..1341)
                     /gene="gmk"
                     /locus_tag="PLR57_02700"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQQ40392.1"
                     /translation="MMKQTREGLLLVVSGPAGVGKGSINQALIQRNSDIRMSVSATTR
                     SPRPGEINGVHYFFKTEEEFKKMIDEGAFLEYMRVFNTHYYGTPKSFVEQELKEGRSV
                     VLEIDVQGAMRVKAAYPDAVLIFIAPPSMSELKTRLIGRGTESAEAVERRFETAYKEL
                     DYVNKYDYVVVNDILDLAIARVEHILSAERCRSVRNGELIEKLMGGRSEL"
     gene            complement(1361..1621)
                     /locus_tag="PLR57_02705"
     CDS             complement(1361..1621)
                     /locus_tag="PLR57_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HQQ40393.1"
                     /translation="MNLRLVNIGFGNIVSADRLIAIVSPESAPIKRIVQEARESGRLI
                     DATCGRRTRAVVITDSDHVILSAIQPETIANRLDTKADEADD"
     gene            complement(1688..2563)
                     /locus_tag="PLR57_02710"
     CDS             complement(1688..2563)
                     /locus_tag="PLR57_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQQ40394.1"
                     /translation="MYSMTGYGKGSASGDGREVTVELKSVNHRYLDVGMRLPRHLSFL
                     EDGIRSLLGARLSRGHIDVFVNYRNLRSDARTVEIDVPLLFAFVSSAKQANETLQLTD
                     DLTLSTALRLPDVSNVREADEDSEAVLALTQSALSAAIDALLGMRLSEGERLKVYLSN
                     CADTVLALTEKISKRAPLVVSEYKTKLDERIQTLLGQVEVDRTRLATEVALFADKASI
                     DEEITRLHSHITEFRSLLNACEPSGRKLDFVVQEMNREFNTIGSKANDGELTSLVLSG
                     KAEIEKIREQVQNIE"
     gene            complement(2694..>3068)
                     /locus_tag="PLR57_02715"
     CDS             complement(2694..>3068)
                     /locus_tag="PLR57_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HQQ40395.1"
                     /translation="ESTIAMQFGATAMHDVTEGGVLGAVWELAYANGCGAEIFSDRIS
                     ICAETRALCAEAGLDPLRLIGSGSLLIACKVGETLVHALKSRGIEAAVIGRAIAGTTC
                     LFDGDAMEEPHADEIYKLYENA"
BASE COUNT          639 a          872 c          857 g          700 t
ORIGIN      
        1 ggggtatggc tcgatgacac gaagcagcgg cttgagcgaa acgggggatg gctgggtctc
       61 atcgcgctcg gtaggcattt cgctcgcctc catcacgcgg atgacgaagc cctcgagctg
      121 gcggttgccg ttgccgaacg gcacgagcac atggctgccc gccggaagcg tgagtccttc
      181 cggcagaaga tacgtaaaga gccggtcgac gtttgcgtgc gcaatatcga caatgacttc
      241 acaggcctgc tgcataccgt tcacctcctc gatgtttctt ttcagggctc tgccctgaaa
      301 ctctctgatt tcgagctcag ggctctgccc tgaaacccgc caaagggatg actccctttg
      361 gaatcccatt tttgccaaaa gcacaaatag cccgcatgcg ggcctgtgca agctgaaacc
      421 gacgcacgga agctaaccgg atcgggttcg gcaagaattc atatcgttcg ttatagcacg
      481 ggaatgacga atcccgtaaa gaagcggggt gtcagtcctg atagaattcc ttcggatatt
      541 ccagattcaa cttatctcga tagagttcat ctacggcgac ggaaacaggc ttgcgcccgt
      601 cgagcttatc tggttccgat tgcaattggc gcgctctctg agacacggcg gtcaccagca
      661 tatagcggca acctgctttc tcgatcagtt cgttcaacgg taaattcatc ataattcgct
      721 cctgcctccc attaatttct cgatcagttc gccgttgcgg acgcttctgc agcgctccgc
      781 ggagaggatg tgctcgactc ttgcaatggc caagtcgagg atatcgttta cgacgacata
      841 atcgtattta ttaacataat ccaattcttt ataagcggtt tcaaaccggc gttcgacggc
      901 ttcggcgctc tcggtgccgc ggccgatcag gcgggttttg agttcgctca tggacggggg
      961 agcgataaag atgagaaccg cgtcgggata ggcggctttg acgcgcatgg cgccctgaac
     1021 gtcgatttcc agtacgacgc tgcggccttc cttgagttcc tgctcgacga agctcttggg
     1081 cgttccgtaa taatgggtgt tgaacacgcg catatattcg agaaacgcgc cttcgtcgat
     1141 catcttttta aactcttctt ccgtcttgaa gaagtaatgc acaccgttga tttcgccggg
     1201 acgcggagaa cgcgtcgttg cggaaacgga catgcggata tcgctgttgc gctgaatcag
     1261 cgcctgattg atcgagcctt tgcctacgcc cgcggggccg gaaacgacca gcaggagacc
     1321 ttctcttgtc tgcttcataa ttcgaacttt cctctcgcgg ttaatcgtcc gcctcgtccg
     1381 ccttggtgtc cagacggttg gcgatggttt caggctggat agcggagagg atcacatgat
     1441 cgctgtccgt aatcacaacc gcgcgggtgc gccgcccgca ggtagcgtcg atcaggcgac
     1501 cgctttcgcg cgcttcctga acgatgcgct tgattggcgc gctttccggg ctgacaatcg
     1561 caatcaggcg gtccgccgaa acgatattgc caaagccgat attcacaagt cgcaggttca
     1621 tcatactaaa ctgctccttc aaaaacgttg tttctgtgcc ggggggctga acggacggca
     1681 gtaccgtcta ttcgatattt tgcacctgtt cgcggatctt ttcgatctcc gccttgccgg
     1741 agagcacaag gctggtgagt tcgccgtcgt tggccttgga gccgatggtg ttgaactcgc
     1801 ggttcatctc ctgcaccaca aagtcgagct ttctgccgga aggttcgcat gcgttcagaa
     1861 gcgaacgaaa ctccgtaata tggctgtgca ggcgggtgat ttcctcgtcg atgctcgcct
     1921 tgtcggcaaa cagcgcaact tcggtagcaa gccgcgtgcg gtccacttcc acctgcccca
     1981 acagggtttg aatgcgctcg tcgagctttg ttttatactc gctgacaacg agcggcgcgc
     2041 gcttggaaat cttttccgta agcgcaagca cagtatccgc gcagttggag aggtaaacct
     2101 tgagccgttc gccttccgaa aggcgcatgc ccagcaacgc gtcgatggcg gcggaaagcg
     2161 cggactgcgt cagcgcgaga accgcttccg aatcttcgtc cgcttcccga acgttggaaa
     2221 catccggcag acggagcgcg gtggaaagcg taaggtcgtc cgtgagctga agcgtctcat
     2281 tggcctgctt tgccgaggaa acgaacgcaa acaggagcgg aacgtcaatt tctaccgtcc
     2341 gcgcgtcgct gcgcagattg cggtaattga cgaaaacgtc gatatgcccg cgcgaaagac
     2401 gagcgccgag cagagagcga ataccgtcct ccagaaacga aagatggcgg gggagacgca
     2461 tgccgacgtc caggtaacga tggttcacac tcttgagttc caccgtaact tcgcgcccgt
     2521 cgccggaagc gctgcccttg ccgtagccgg tcatgctgta catcaaaaat tcacccccaa
     2581 gtcgcaatta taaagtatat catgcccgga gcattcacac aagcgataga ttttgccgga
     2641 cacgtcaaat acggttcaaa ggtttacgcg caactgcttt atttcgccct gcattacgcg
     2701 ttttcataca acttatagat ttcgtccgcg tgcggttctt ccattgcgtc gccgtcaaag
     2761 aggcaggtgg ttcccgcaat tgcacgcccg atgaccgcgg cttcgattcc gcgggacttc
     2821 agcgcgtgca ccagcgtttc gcctactttg caggcaatca agaggcttcc gctgccgatg
     2881 agcctgagcg ggtcgagtcc tgcttcggcg cagagcgcgc gtgtttccgc acagatggaa
     2941 atccggtcgg aaaagatttc cgcgccgcat ccgttggcgt aagccagctc ccagaccgcg
     3001 ccgagaacgc cgccttcggt cacgtcgtgc atggcggttg ccccaaactg catggcgatc
     3061 gtgctttc
//
LOCUS       DAOMGY010000122         3058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_34302_length_3058_cov_3.176490, whole genome shotgun sequence.
ACCESSION   DAOMGY010000122 DAOMGY010000000
VERSION     DAOMGY010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34302_length_3058_cov_3.176490"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLR57_02720"
     CDS             <1..766
                     /locus_tag="PLR57_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HQQ40396.1"
                     /translation="FGDERRIRAILGSLGADHKAFHIVEPGDGGADASAEAAIQAVID
                     CEADFLMKGAMSTSDLLKHVVRSPLVQGIPLSHTMIFEVPAYGKLLVNTDGGMNPAPD
                     LARKKAILENAAVLLKTLGYETITAACVSGSEVVSEKIPSTTDAKALAEMDWSRYNME
                     VYGPVGLDLAISEQARVHKGYNVPGAKDADILLMPNYETANCFGKALTYFAGARSAGL
                     VVGARCHIVLVSRADPAETKLSSLALGAIAAKGETT"
     gene            766..2547
                     /gene="iorA"
                     /locus_tag="PLR57_02725"
     CDS             766..2547
                     /gene="iorA"
                     /locus_tag="PLR57_02725"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HQQ40397.1"
                     /translation="MESGQKHLLTGNEAIARGAYEAGAVVCSAYPGTPSTEIFETLAQ
                     YKDSLYAEWAPNEKVAVEVAYGASMANVRSLSAMKHVGVNVAADPIFTVAYNGVGAGF
                     VIVSADDPDMHSSQNEQDNRRYAPFAKIPLVEPSDSQECKDFMKEAYEISERFDLPVL
                     FRVTTRICHSKSLVEFGKREERPVAPYARNIPKFVCTPANAKRHRPMLEAKLEHLAEY
                     AETSPLNRMELKGKKIGVVTSSIAYQYAKEAFPEDTSFLKLGFTWPLPIRLIRTFAEQ
                     VETLYVVEELDPFLEDQIKAAGIFCIGKALIPNMYELNPEVVREAVFGEKTRVVRVGG
                     GTVPRPPALCPGCPHRGFFYVLSKRKDAVIAGDIGCYTLGCAAPLSAMDTVVCMGGGF
                     SVAMGMAKAFERSGQTGKKVFGVVGDSTFFHSGMTGAVEILYNAGSVIPCVLDNSITG
                     MTGHQDNPGTGYTLSGEIATRVSIEEVLKSFGYQNVIVVDPQDIKAMQKAVDDAVAST
                     VPAAIVARRPCLLIKRMKHDIGKCVVNTETCRGCKLCLKVACPALHLNNGKAEIDRNL
                     CVGCRVCAQVCSFGAITREGADNQKGE"
     gene            2554..>3058
                     /locus_tag="PLR57_02730"
     CDS             2554..>3058
                     /locus_tag="PLR57_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HQQ40398.1"
                     /translation="MATNDTKSILLAGVGGQGTILVSKVLTHGLVSAGYDVKMSEVHG
                     MAQRGGSASTQVRYGTKVYSPIIGRGEADVLVAFEKMEAARYAEFLKVGGTAVINDYA
                     IMPLPVATGMADYPEGTIEAMQMSFPVIVIPASRIAEELGNPKCMNIVLLGALVNAFH
                     LEQIDWNA"
BASE COUNT          656 a          909 c          920 g          573 t
ORIGIN      
        1 ctttggcgac gagcgcagaa tccgcgcgat actcggttcg cttggcgccg atcataaggc
       61 gttccacatc gttgaaccgg gtgacggcgg cgcggacgca agcgcggaag ccgcgattca
      121 ggcggtgatc gactgcgaag cggatttttt gatgaagggc gcaatgagca cgtccgacct
      181 gctcaagcac gtcgttcgtt cgccgctggt gcagggcatt ccgctctccc acacgatgat
      241 ttttgaagtg cctgcttacg gcaagctgct ggtcaatacg gacggcggca tgaaccccgc
      301 gcccgatctc gcgcgaaaga aagcgattct ggaaaacgcc gcggttcttc taaaaacgtt
      361 ggggtacgaa accattaccg ctgcgtgcgt gagcggcagc gaagtcgtca gcgaaaaaat
      421 tccttccaca acggacgcaa aagccctcgc agagatggac tggtcgcgct acaatatgga
      481 agtatacggg cctgtcgggc tcgacctcgc catcagcgag caggcgcgcg ttcataaggg
      541 ctataatgtg cccggcgcaa aggacgcgga catcctgctc atgcccaact acgaaacggc
      601 gaactgcttc ggaaaagcgc ttacgtactt tgccggcgcg cgttcggcgg ggctggtggt
      661 tggcgcgcgg tgccacattg ttctggtatc ccgcgcagac ccggcggaaa cgaaattatc
      721 gtccctcgcg cttggcgcga tcgcggcgaa aggagaaact acctgatgga atccggtcaa
      781 aaacaccttc tcacgggcaa cgaggcaatc gcgcgcggcg cttacgaagc gggcgcagtc
      841 gtctgctccg cctaccccgg cacgccgagc acggagatct tcgaaacgct cgcgcagtac
      901 aaggactcgc tctacgcgga gtgggcgccg aacgaaaagg tcgccgtaga agttgcgtac
      961 ggcgcgagca tggcaaacgt tcgctcactt tcggccatga agcatgtggg cgtcaacgtt
     1021 gccgccgacc cgatctttac cgtcgcctac aacggtgttg gcgcggggtt tgtgattgtc
     1081 agcgcggacg atccggacat gcactcttcc caaaacgagc aggataaccg ccgctacgcc
     1141 ccctttgcca agattccgct tgtggagccc agcgattcgc aggaatgcaa ggatttcatg
     1201 aaggaagcct acgaaatttc ggagcgcttc gacctgcccg tgctgtttcg ggtgaccacg
     1261 cgcatttgcc acagcaagag cctggtcgaa tttggcaaac gggaggaacg ccccgttgcc
     1321 ccatatgcgc gcaacattcc gaagttcgtc tgcacgcccg ccaacgccaa gcgccaccgc
     1381 ccgatgctgg aagcgaagct tgagcatctc gcggaatacg ccgagaccag tccgctcaac
     1441 cgtatggagc tcaagggcaa aaaaatcggc gtagtcacgt cttccattgc ctaccagtac
     1501 gcaaaggaag ccttcccgga agatacgtcg ttcctcaagc tcggctttac ctggccgctt
     1561 cctatccggc tcatccgcac gtttgccgag caggtggaaa cgctgtatgt cgttgaagag
     1621 ctcgacccgt tcctggagga tcagatcaag gccgcgggca tcttctgcat cggcaaggca
     1681 ctgattccga atatgtacga actcaacccg gaagttgtcc gcgaagcggt gttcggcgaa
     1741 aagacgcgcg ttgtccgcgt tggcggcggc accgtcccgc gtcccccggc gctctgcccg
     1801 ggctgtccgc acagggggtt cttctatgtg ctcagcaagc gcaaggatgc cgtcatcgct
     1861 ggcgatatcg gctgctacac gctcggctgc gcagctcccc tttcggcaat ggatacggtc
     1921 gtctgcatgg gcggcgggtt ctccgttgcc atgggcatgg caaaagcctt tgagcggagc
     1981 ggccagaccg gcaaaaaggt ctttggcgtg gttggagatt ccacgttttt ccacagcggc
     2041 atgaccggcg cggttgagat tctctacaac gccggaagcg tcatcccctg cgtgctggac
     2101 aactccatca ccggcatgac gggtcatcag gacaaccccg gcacgggcta tacgctctcc
     2161 ggcgaaatcg ccacgcgcgt ttcgattgaa gaagtgctca aaagctttgg ctaccagaac
     2221 gtaatcgtcg tcgatccgca ggatatcaag gccatgcaga aggccgttga cgacgcggtg
     2281 gcaagcaccg tgcccgcggc aatcgtagcg cgaaggccgt gccttttgat caagcgcatg
     2341 aagcacgata tcggcaaatg cgtggtcaac acggaaacct gccgcggctg caaactctgc
     2401 ttaaaagtcg cctgcccggc gctgcacctc aataacggca aagcggaaat cgaccgcaac
     2461 ctctgcgtcg gctgcagggt ctgcgcgcag gtttgctcct ttggcgcgat tacgcgcgaa
     2521 ggcgcggaca accagaaagg cgagtgagcg aatatggcga cgaatgatac caaaagcatc
     2581 cttctggcgg gcgttggcgg gcagggcacg attttagtga gcaaagtcct gacgcacggg
     2641 ctcgtttccg caggatacga cgtaaagatg agcgaagtgc acggcatggc gcagcgcggc
     2701 ggctcggctt ccacgcaggt gcgctacggc accaaggtct actcccctat catcggtcgc
     2761 ggcgaggcgg atgtgctggt cgcgtttgaa aaaatggaag ccgcgcggta cgcggaattc
     2821 ctgaaagtcg gcggaaccgc cgtaatcaac gattacgcca tcatgccgct tccggtcgcc
     2881 acgggcatgg cggactaccc cgaaggcacg atcgaagcca tgcagatgtc gtttcccgtg
     2941 atcgtgattc ctgcaagccg catcgccgaa gagctcggca atccgaaatg catgaatatc
     3001 gtacttctcg gcgcactggt caatgcgttt catctggaac aaatcgactg gaacgccg
//
LOCUS       DAOMGY010000123         3050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_34420_length_3050_cov_3.346578, whole genome shotgun sequence.
ACCESSION   DAOMGY010000123 DAOMGY010000000
VERSION     DAOMGY010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34420_length_3050_cov_3.346578"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1121
                     /locus_tag="PLR57_02735"
     CDS             219..1121
                     /locus_tag="PLR57_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40399.1"
                     /translation="MSAIVETKNLTKYYGKARGIVDVTLSIEEGEIFGFIGPNGAGKS
                     TTIRTLLSLIYKTGGEARIFGLDTEKHNVEILREVGYLPGEVFYYENMRAIDLFRYSA
                     SFYKKDCSRRIEELAGILQVNLKQKIEDMSLGNKKKVGIIQGLLHSPRLIILDEPTSG
                     LDPLMQKTFFDLIREENQRGATVLFSSHILSEVQRICDRVAIIKEGRIVAQRSMAELR
                     ENAVKNVSLTFRTPEDAAGFALEGAQDLEIAQAGVSFLYRGAIGSLLGDLAKRPLVNA
                     DISEPTLEEIFLHYYADGAEASNT"
     gene            1118..1918
                     /locus_tag="PLR57_02740"
     CDS             1118..1918
                     /locus_tag="PLR57_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQQ40400.1"
                     /translation="MNIFAYECKSQLRSFINWTVGIVGGFLLFVAVFYGAFMDSKAAV
                     EQALANLPPAFAALFGVHVQSMFTFGGFFQFGYTYLSVVAAIMASVTGIAVFAREKRN
                     KCADFLLVKPLRRSEIFAQKLFACFALILALNVLFLGAVLAAYAVNGVGAEGYARVAL
                     AGSSLFFVQLFYLAVAVLYATLAKKVRSVSGAGMALGFSGFLLMALYSLTEEQAVRWV
                     SPLQYFSPASVFSTGGYETKYVLVAVCAAVACLLAAFWRFCRSDARAD"
     gene            1934..2722
                     /locus_tag="PLR57_02745"
     CDS             1934..2722
                     /locus_tag="PLR57_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQQ40401.1"
                     /translation="MNLLKRELRANWKAFFFWSIGMFVLCFAGVVKFESYTASGSMME
                     LLNSFPRIVLAVMGAVGVDMGSLNGYIALMFYYVLICACIYALHLGSFAVSRESVDKT
                     YEFLFTKPRSRSHLLGVKIIAAYIYFAVFCLVSGAFVLLAVQTLGSGSGVEGEVMLCV
                     LGVFFVGSMFLSLAACFSAYAMRPEKGAALGNLAFLAAFVLGVVYNLLEKPGLLRLVS
                     PISYFPASELAEKTLSLPYSALALVLSGTFLVLAFRRFDKKDLL"
BASE COUNT          600 a          782 c          899 g          769 t
ORIGIN      
        1 aaacaggggg cgttccgccc cctaacccct gacttagggg cgactgcccc taagattccc
       61 atataaatcc cgcgcgagaa attgcgcggg ttttcgtttt gcacaaaggg ttgcataata
      121 ataatcaatg atgcatactg gtgttaatta acaagtcaaa agtaaacgag caattcgaat
      181 tttctagcaa aatcaatcga atacaggaag ggcaaactat gagcgcgatt gtggaaacga
      241 aaaacctgac caagtactac ggtaaggcgc ggggcattgt ggacgttacc ctttccattg
      301 aggaaggcga aatcttcggt ttcatcggcc ccaacggcgc gggaaagtcc accaccatcc
      361 gtacgctcct ctcgctgatc tacaagaccg gcggggaagc gcgcatcttt ggtctggaca
      421 cggagaagca caacgtggaa atcctccgtg aagtcggcta cctgcccggc gaggtgtttt
      481 actatgaaaa catgcgcgcc atcgacctct tccgctattc cgcgagcttc tacaaaaagg
      541 actgctcgcg gcgcattgag gaactggcgg gaatcctgca agtcaacctc aaacagaaga
      601 ttgaggacat gagcctcggc aataaaaaga aggttggcat cattcagggt ctgctccatt
      661 cgccgcggct gatcattctg gacgaaccga ccagcggcct cgacccgctc atgcaaaaga
      721 ccttcttcga tttgatccgc gaggagaacc agcgtggggc gacggtgctc ttttcgtcgc
      781 acatcctcag cgaagtgcag cgcatttgcg accgcgtggc catcattaag gaagggcgca
      841 ttgtggcgca gcggagcatg gccgagctgc gcgaaaacgc ggtgaaaaac gtaagcctga
      901 ccttccgtac gccggaggac gcggcggggt ttgcgttgga aggcgcgcag gatcttgaga
      961 ttgcgcaggc gggcgtgagc tttctgtatc gcggggcgat cggttcgctt cttggcgatc
     1021 tggcaaagcg tccgctcgtc aacgcggaca tttcggagcc gacgctggaa gagatcttcc
     1081 tccactatta tgcggacgga gcggaggcga gcaacacttg aacatctttg cctatgaatg
     1141 caaatctcag ctgaggagct ttatcaactg gacggtcggt attgtcggcg ggtttctgtt
     1201 gtttgtcgcg gtgttttacg gcgcgtttat ggacagcaaa gccgccgtcg agcaggcgct
     1261 tgcaaacctg ccgcctgcgt ttgcggcgct gttcggcgtg catgtgcaga gcatgttcac
     1321 gttcggcggg tttttccagt ttggttatac ctatctcagc gtcgtggcgg cgattatggc
     1381 ctcggttaca ggaattgccg tctttgcgcg cgaaaagcgc aataagtgcg cggacttcct
     1441 gctcgtaaaa cctctgcgcc gcagcgaaat ctttgcgcaa aagctctttg cgtgctttgc
     1501 gctgattctt gcgctcaacg tgctgttcct cggcgccgtc ctcgcggcgt acgcggtcaa
     1561 cggcgtggga gcggaaggct atgcgcgtgt ggcgctcgcg ggatcgtcgc tcttttttgt
     1621 gcaactgttt tatctggccg tcgcggtgct gtatgccacg cttgccaaaa aagtgcgttc
     1681 cgtctccggc gcgggcatgg cgcttgggtt ttcgggattt ctcctgatgg cgctctattc
     1741 gctcaccgag gagcaagctg ttcgctgggt ttcaccgctg cagtattttt ctccggcgtc
     1801 cgtgttctcc acgggcgggt atgaaacgaa gtatgtgctg gttgccgtgt gcgccgcggt
     1861 tgcctgcctg ctggccgcgt tctggcgttt ctgccgcagc gacgcgcgcg cggactagag
     1921 gaaggagcgt tctatgaatc ttttaaaacg tgaactgcgc gccaactgga aagcgttctt
     1981 cttctggtcg atcggtatgt ttgtgctctg ctttgcgggc gtcgtcaagt ttgaaagcta
     2041 caccgccagc ggaagcatga tggaactgct caactcgttt ccgcgtatcg tactggctgt
     2101 gatgggcgcg gtcggcgtgg acatgggttc gctgaacggg tatatcgcac tgatgttcta
     2161 ctatgtgctc atttgcgcgt gcatctatgc tttgcatctg ggctcgtttg ccgtttcgcg
     2221 ggaaagcgtg gacaagacct atgaattcct tttcacaaag ccccgctcgc gctcgcatct
     2281 gctcggcgta aaaattattg cggcgtatat atactttgcg gtgttctgcc ttgtcagcgg
     2341 cgcgtttgtg ctcttggcgg tgcagacgct tggttcgggc agcggagtag agggcgaagt
     2401 gatgctctgc gtgcttggcg tgttctttgt gggctctatg ttcctctcgt tggcggcgtg
     2461 tttttccgcg tatgccatgc ggccggaaaa gggcgcggcg ttagggaacc tagcgttcct
     2521 tgccgcgttc gtgctcggcg ttgtctacaa tctgctcgaa aagccgggtt tactgcggct
     2581 cgtttctcct attagctact ttcccgcttc cgaacttgcg gaaaagacgc tcagcctgcc
     2641 gtattcggcg ctggcgctcg tgctttcggg cacatttctc gtgcttgcgt ttcgccggtt
     2701 cgacaaaaag gatttgctgt aaggtggaag atgtcggggg ctttcagccc ccaagatcga
     2761 aaaacagggg gctttcagcc cccaaacccc tgacttaggg gcgactgccc ctaagaaccc
     2821 cacttttatc ccgcgcgaga agttgcgcgg gtttttcgtt gttttttaga tattgttaag
     2881 caaatatgtg cggcgggata agcccgcgta aaatttcgaa aattacattt taaaatggtg
     2941 aaggcgtagt aatcccgctt gttccgaatg gtttagtttg ctatactgca ggtattggga
     3001 cacatcgatt caacatctgc gaaaactgaa tttatgtatg ggagagggtg
//
LOCUS       DAOMGY010000124         3029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_34761_length_3029_cov_3.783457, whole genome shotgun sequence.
ACCESSION   DAOMGY010000124 DAOMGY010000000
VERSION     DAOMGY010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34761_length_3029_cov_3.783457"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..671)
                     /locus_tag="PLR57_02750"
     CDS             complement(186..671)
                     /locus_tag="PLR57_02750"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HQQ40402.1"
                     /translation="MIQLVRCDDRLIHGQCVVRVISDFGIGHIVVCDDFVAGNAVLKN
                     VFLLAAPSGIKTEILSVEQAAARMDALQSEAANVLILVRSPETALSLYLAAPQLKKEL
                     NIGPMSNRPNAKKATMYCSLTKDEAVAVHQLAFLGVRVYFNQVISQKAVEWAEIEQQF
                     K"
     gene            complement(668..1093)
                     /locus_tag="PLR57_02755"
     CDS             complement(668..1093)
                     /locus_tag="PLR57_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HQQ40403.1"
                     /translation="MTAIIVSAHAALAEGLLGAVSMIAGEQEQLYSVPFLPGESLTDL
                     IERVSRQITDSGAEQALVLTDLFGASPANAASAALFSVPAETAVVTGVNLAALLEAAL
                     TRSDAEPLNQFAARVAQAGRENIRLITKETVLNAGGADA"
     gene            complement(1157..1867)
                     /locus_tag="PLR57_02760"
     CDS             complement(1157..1867)
                     /locus_tag="PLR57_02760"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQQ40404.1"
                     /translation="MSKNDQPLFKQIIASINEGIESGAYARGSAIPSEPELCRVFDTT
                     RMTVRRAIDALVNEGKLYRVQGKGTFVSHIELQKTYQKHGFTSNMISLGIHPSSKVLS
                     MGVCDPDDAVKASLEVEPGEPLFCLKRIRMADLEPIAIERVWISLKRFPKLPDYDYSE
                     TSLYEVLRVEYGLEAGYSRQKLNAVMVEGEDAQILFGAKKGVAMRMRNVDYDKNLRPI
                     AAADSIYHGTKYTFDIVI"
     gene            2220..>3029
                     /locus_tag="PLR57_02765"
     CDS             2220..>3029
                     /locus_tag="PLR57_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ40405.1"
                     /translation="MDCILTAKRLLLTDGELEHGAALVQNGRISAVGTAEEFGGVDLP
                     LLDCGEHILSPGFFDLHIHGCMGRVADESEDAVNLLAGYLPRTGTTSFLATAMTARGC
                     KHAAGAIAAQKVGKVPGAMIRGIYMEGPFLSPRKLAGAEGADQDIVAPSLLALEEILA
                     IGDGNIRVMGLGVELDGASEIMRALKSRGIVVSAAHTRAEYADILAAKANGMTHATHL
                     YNVMSGLHHRRPGVVGAVLTCDDITTELICDGVHLHPAAIDVALRCKGSDRI"
BASE COUNT          656 a          824 c          896 g          653 t
ORIGIN      
        1 gaacgccgcg atgaggattg cctgaaacag cgtcatgatc tttgcctcct gaatgcttga
       61 gttaggtggg gttcccaaag gggtgaatac ccctttggca ggagcgcgag ggcggagcct
      121 tcgcaaggtt taaaatcaaa cgtgtgaacc gaaaaaacaa gacgagggct gagccctcga
      181 acaaactact taaattgctg ttcaatttcc gcccactcga cggctttctg gctgatgacc
      241 tgattaaagt acacccgcac gcccaagaac gccagctgat ggacggcgac ggcctcgtcc
      301 tttgtgagac tgcagtacat cgttgctttt ttcgcgttgg ggcggttgct catggggccg
      361 atgttcagct cttttttgag ctgcggcgcg gcaaggtaga gcgaaagcgc ggtttccggt
      421 gagcggacga gaatcagcac gttggcggct tccgactgga gcgcgtccat gcgggcggcg
      481 gcctgttcaa cggaaaggat ttccgtctta atgccggacg gcgcggcgag cagaaagacg
      541 tttttgagca cggcgttgcc cgcaacaaaa tcgtcgcaaa ctacgatatg cccgatgccg
      601 aagtcggaga tgacgcgcac cacgcactgg ccgtggatga gccgatcgtc gcagcggaca
      661 agctgaatca tgcgtctgct cctcccgcgt tcaaaacggt ttcttttgtg atgagacgga
      721 tgttctcgcg cccggcctgc gcaacgcgcg cggcgaattg attcagaggt tctgcgtcgg
      781 aacgcgtgag cgccgcttcc aacagggcgg cgagattcac gcccgtgacc acggcggttt
      841 cagccggaac agagaacaat gcggcggaag cagcgttcgc gggcgacgcg ccgaacagat
      901 cggtcaaaac aagcgcttgt tccgcgccgg aatccgtaat ttgccgggat acgcgttcaa
      961 tcaaatccgt caggctttcg cccggcagaa aaggaacgct gtagagctgc tcctgctcgc
     1021 ccgcgatcat ggaaaccgcg ccgagaagcc cttcggcaag cgccgcatgc gcggaaacga
     1081 tgatggcggt catattcgta cccccgcagg atttattcta tccaaatgtt ctagcgcgtt
     1141 ataaaccagt taaaaatcag atcacaatat caaacgtgta cttcgttccg tggtaaatcg
     1201 aatccgctgc ggcgatcggg cgaaggtttt tgtcgtaatc gacgttgcgc atgcgcatgg
     1261 caacgccttt ttttgcgcca aagaggattt gcgcgtcctc gccttccacc atgacggcgt
     1321 tgagcttctg ccgcgaatag cccgcttcca gcccgtattc cacgcggagc acttcgtaaa
     1381 gcgacgtttc ggaataatcg tagtcgggca gtttcggaaa gcgcttcagg gaaatccata
     1441 cgcgttcaat ggcgattggc tcaaggtccg ccatgcggat gcgcttaagg cagaacagcg
     1501 gttcgcccgg ctccacttcc agactggctt taacggcgtc gtccggatcg cagacgccca
     1561 tggagagcac cttgctggag ggatggatgc caagcgagat catgttgctc gtaaagccgt
     1621 gtttttgata ggttttctgc agttcgatat gggaaacaaa cgtgcctttt ccctgtacac
     1681 ggtagagttt gccttcgttc accagcgcgt caatggcgcg ccggacggtc atgcgcgtgg
     1741 tgtcgaacac gcggcagagt tcaggttcgg acggaatggc agatccgcgc gcgtacgcgc
     1801 cgctctcgat cccctcgttg atgctggcga tgatctgctt gaataacggt tggtcgtttt
     1861 tgctcaaagc gttcatcccc ttttcgttgt tcttttcttc atcatacagc ggttttttgt
     1921 agaacggtag tagttttttt atcggaacga aaaagtggtt cggcgaagag tgaataacga
     1981 acggcaaaaa taattttcaa agttgtatat acaagatata gacattctat cattcttttg
     2041 ctatattgca agcagaagtt acagtttcaa acgaaaaatc gcaaacaaac gacccgatcc
     2101 aaaccaatcc ttttgatagc ctcttccgct gctgagacct tgataaccgt cttcgcggac
     2161 gcgtctgcgt aataataggc tgtattttga acgcttcaga gaaggggggg cgatgaacca
     2221 tggactgcat cctgacggca aagcgtttac tcctgacgga cggcgaactg gagcacggcg
     2281 cggcgctcgt tcaaaacggg cgaatttccg ccgtcggaac tgcggaggag tttggcggcg
     2341 tggatttgcc gctgctggac tgcggcgaac atattctcag ccccggcttc ttcgacctgc
     2401 atatccacgg ctgcatgggg cgcgttgcgg acgagagcga ggacgcggtg aacctgctcg
     2461 cgggctatct gccccggacg ggtacgacct cgtttctggc gacagccatg accgcgcgcg
     2521 gctgcaaaca tgccgcaggc gcgattgccg cgcaaaaagt gggcaaagtg cccggggcta
     2581 tgattcgcgg tatctatatg gaagggccgt tcctctcccc gcgcaaactc gcgggcgcgg
     2641 aaggcgcgga tcaggatatc gttgcaccga gcctcctcgc gctggaggag attctcgcaa
     2701 tcggcgacgg aaacatccgc gttatggggc tgggcgtgga gctggatgga gcaagcgaga
     2761 tcatgcgcgc gctgaaaagc cgcggaattg tcgtttccgc tgcgcacacc agggcggaat
     2821 acgcggacat tttggcggca aaggcgaacg ggatgaccca cgccacgcac ctctataacg
     2881 tgatgagtgg cctgcatcac cgccgccccg gcgtggtcgg tgcggtgctg acctgcgacg
     2941 atatcactac cgaactcatc tgcgacggcg tgcacctgca tcccgcggca atcgacgtgg
     3001 ctctccgctg caaaggcagc gacaggatc
//
LOCUS       DAOMGY010000125         3026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_34818_length_3026_cov_3.048805, whole genome shotgun sequence.
ACCESSION   DAOMGY010000125 DAOMGY010000000
VERSION     DAOMGY010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34818_length_3026_cov_3.048805"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLR57_02770"
     CDS             <1..303
                     /locus_tag="PLR57_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HQQ40406.1"
                     /translation="NANRYFLRLLGLLGRTIPSGGGLLLTPCNDIHTFGMRYPIDAVY
                     LDAAGRVLRVDDSLRAGKAWPAQRGAKRVLELPAGAAKERNIRVNDILEVIPWTKS"
     gene            288..1148
                     /locus_tag="PLR57_02775"
     CDS             288..1148
                     /locus_tag="PLR57_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HQQ40407.1"
                     /translation="MDKELRRHEESVDALRNRMDADARFHRVLSGYDPDEVREYLEET
                     RKTFSQQAKAAKREMESVFAELESAKSEIDSRNCAIRTLKEALSQREKELTDATVRIT
                     TLLQNLKKYEAEREGYARLRAAVDGVRVTNERAQALEQEVQQLRTSLSQAAGLATSWR
                     DERTLLTSENARMSAEIEQLRSEVLRVTAERDQARSVAYMAAMNNRAPAAPVRQEAPV
                     YAPPEPAGIPPQIADRLAETFAEAYALISQFKSGSEPQAEPPVQRPTAPPKMQVLRPD
                     GSFGERGFGK"
     gene            complement(1240..2115)
                     /gene="aguB"
                     /locus_tag="PLR57_02780"
     CDS             complement(1240..2115)
                     /gene="aguB"
                     /locus_tag="PLR57_02780"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014425421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HQQ40408.1"
                     /translation="MSKITVAAVQMRMTNSPAENLAKAEAFVREAAKRGAQIVLLPEL
                     FERPYFCQERRYEYLDYALPVSENPAVLRMQAVAKELGIVLPVSFYEREQNRLYNSVA
                     MIDADGSLLGVYRKTHIPDDHFYQEKFYFSPGNTGFRVFETRFGRVGVGICWDQWFPE
                     AARAMALLGADILLYPTAIGSEPILTVDSMPHWRRVMQGHAAANILPVAAANRVGEEK
                     VLPCAENGNQSSALTFYGSSFLTDETGEIVASADRESEGVLYAEYDFEQIKQARLSWG
                     LFRDRRAECYKELVR"
     gene            complement(2112..>3026)
                     /locus_tag="PLR57_02785"
     CDS             complement(2112..>3026)
                     /locus_tag="PLR57_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HQQ40409.1"
                     /translation="EHYSEALERAGAFAALLGIPSNDAWARDIGPTFVTNGEHVCGVS
                     WRFNAWGGEVDGLYADWQEDDTFAEAFCAAIDADCCRTEDFVLEGGSICSDGEGTLLV
                     TEACLLSAGRNPNLAKEQIERKLKDFLGIEKVLWLPRGIYNDETNEHVDNICAFLAPG
                     EVVLAWTDDESDPQYALSRADYDYLSSVTDALGRRLVIHKLPIPDHPVCVMKADLANF
                     VFEQGEDTRTDGERLAASYVNFYFSNDSVLLPQFAGENAASDVRAVELMRRLCPNRRI
                     VPIDSRPILLGGGNIHCVTQQIPKGALK"
BASE COUNT          616 a          955 c          865 g          590 t
ORIGIN      
        1 aatgccaacc gctatttcct gcgccttctg ggcttgctcg ggcgcacgat tccttccggc
       61 ggcggcctgc tgttgactcc ctgcaacgat atacatacct tcggcatgcg ctaccccatc
      121 gacgcggtgt atctcgacgc tgccgggcgc gttctccgcg tggacgactc tctgcgggcc
      181 ggaaaagcct ggcccgcgca gcgcggcgca aaacgcgtgc tggaactgcc cgcgggagcc
      241 gcaaaggagc ggaatattcg cgtgaacgat attctggagg tgattccgtg gacaaagagc
      301 tgaggcggca cgaggaaagc gttgacgctc tgcgcaaccg catggacgcc gacgcgcggt
      361 tccaccgggt tctttccggc tacgatccgg acgaagtacg cgaatatctg gaagaaacga
      421 gaaaaacatt ttcacagcag gccaaggcgg caaaacgcga gatggagtcc gtctttgcag
      481 agctggaatc cgcaaagagc gaaatcgact cccgcaactg cgccatccgc acgctgaagg
      541 aagcgctttc tcagcgggaa aaggagctta cggacgcaac cgttcgcatc accacgctcc
      601 tgcagaatct gaagaaatac gaagccgaac gcgaagggta cgctcgcctc cgcgcggcgg
      661 tggacggcgt acgcgtgacc aacgaacgcg cgcaggcgct ggagcaggaa gtgcagcagc
      721 ttcgtacatc gctttcgcag gcggcggggc ttgcaacctc ctggagggac gagagaaccc
      781 ttctgaccag cgaaaacgcc cgcatgagcg cggagatcga acagctgaga agcgaagtcc
      841 tgcgcgtcac cgcagagcgc gatcaggcgc gttccgtggc ctacatggcc gctatgaaca
      901 accgcgcgcc cgcggctccc gtaaggcagg aagcgcccgt ttatgctccg ccggaacccg
      961 ccggaattcc gccccaaatc gcggatcgcc ttgcggagac attcgcggaa gcgtatgcgc
     1021 tcatcagtca gttcaaaagc ggttcggaac cacaggccga gccccccgtg cagcggccca
     1081 ccgcccctcc caagatgcag gttctccggc ccgacggcag ttttggcgag cgcggattcg
     1141 gaaaataatt gctgattgag tataaaacaa aagcgcggct taccgcgctt tttgcttgtt
     1201 ctggattgtt gccctaatta taggccgaac cgtcaaggtt caccgaacca attctttata
     1261 gcactccgcc ctgcggtcac gaaacagccc ccagcttaat cgcgcctgct tgatctgctc
     1321 gaaatcgtat tcggcataga gtaccccttc gctctcgcga tctgccgagg cgacaatctc
     1381 ccccgtttcg tccgtcaaaa acgacgagcc gtagaacgtc agcgcgctgg actggttgcc
     1441 gttttccgcg cagggtaaaa ctttctcttc tcccacgcgg tttgccgctg cgacgggcag
     1501 aatattcgcc gccgcgtgcc cctgcataac ccttctccag tgcggcatgc tgtccacggt
     1561 gagaatcggt tccgagccga ttgcggttgg atacagcaga atatccgcgc caagcagcgc
     1621 cattgctcgc gcggcttccg gaaaccactg atcccagcag atgcccacgc cgacgcgtcc
     1681 gaaacgcgtt tcaaacaccc gaaagcccgt gttgccgggt gaaaagtaga acttttcctg
     1741 ataaaaatgg tcgtccggga tatgcgtctt gcggtacacg ccaagcaggc tcccgtccgc
     1801 gtcgatcatg gcgacggaat tgtacaagcg gttttgctcc cgctcgtaaa agctcacggg
     1861 caggacaatg ccgagttctt ttgcaaccgc ctgcatgcgc aataccgccg gattttccga
     1921 aacgggcagc gcgtagtcca gatattcata ccggcgttcc tggcagaaat acgggcgctc
     1981 gaacagctcc ggcaggagca caatctgcgc gccgcgcttt gccgcttcgc gcacgaaggc
     2041 ttccgctttc gcgaggtttt cagccgggct gttcgtcatg cgcatctgca cggcggcaac
     2101 ggtgatcttg ctcattttaa cgctcctttc gggatctgct gcgtcacgca atggatgttt
     2161 ccgccgccaa gcaggatggg gcgggaatcg atcggcacaa tccttcggtt cgggcagagg
     2221 cggcgcatga gctcaaccgc gcgcacgtcg gaagcggcgt tttctcccgc gaactgcggc
     2281 agaagaacgg aatcgttgga aaagtaaaag tttacgtagc tcgcggcaag gcgttcgcca
     2341 tctgtacgcg tatcctcccc ctgttcgaat acgaagttcg ccaaatccgc tttcattacg
     2401 caaaccggat ggtctggaat tggcagttta tgaatcacga gccgccgccc cagtgcgtcc
     2461 gtaacgcttg aaagatagtc gtagtccgcg cggctcagcg catactgcgg gtcgctctcg
     2521 tcgtccgtcc acgccagcac gacttcgccc ggcgcgagga atgcgcaaat attgtccaca
     2581 tgttcgttcg tctcgtcgtt atagattccc cgcggcagcc agagcacctt ctcaatacca
     2641 agaaaatcct tgagtttgcg ctcaatctgc tcttttgcaa ggttcgggtt tcgccccgcg
     2701 ctgagcaggc acgcttccgt cacgagcagg gtgccttccc cgtccgagca gatggaaccg
     2761 ccctccagca cgaagtcttc cgttcggcag cagtccgcgt cgattgcggc gcagaacgcc
     2821 tccgcgaatg tgtcgtcctc ttgccagtcc gcgtagaggc catccacttc gccgccccag
     2881 gcgttgaagc gccagcttac gccgcacacg tgctcgccgt ttgtcacgaa cgtagggccg
     2941 atatcccgcg cccatgcgtc gtttgagggg attcccagca gcgcggcgaa cgcgcccgcg
     3001 cgttccagcg cttcgctgta atgctc
//
LOCUS       DAOMGY010000126         3020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_34920_length_3020_cov_3.457336, whole genome shotgun sequence.
ACCESSION   DAOMGY010000126 DAOMGY010000000
VERSION     DAOMGY010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34920_length_3020_cov_3.457336"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /gene="dnaK"
                     /locus_tag="PLR57_02790"
     CDS             complement(<1..1349)
                     /gene="dnaK"
                     /locus_tag="PLR57_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HQQ40410.1"
                     /translation="MGKIIGIDLGTTNSCVAFLEGGEPVVIPNSEGARTTPSVVAFKD
                     GERMVGNIAKRQAITNPDRTVASIKRDMGSARKVRIEGKDYTPQEISAMILQKLKQDA
                     EAYLGSTVTQAVITVPAYFTDSQRQATKDAGRIAGLEVLRIINEPTAAALAYGMDKEN
                     NQKILVYDLGGGTFDVSILEIGDGVFQVLATAGNNRLGGDDFDQLIIDYIAEDFKRNN
                     GIDLRQDKMALQRLKEAAEKAKIELSGVAQTTINLPFITMDKSGPKHLDITITRAKFN
                     ELINDLVEQTKTLVQKCLTDAGLSASQVDKVILVGGSSRIPVVQEMVKKITGKDPFKG
                     INPDECVAIGAAIQGGVLGGEVKDILLLDVTPLSLGIETVGGVFTRLIDRNTTIPTKK
                     SQIFSTAADGQTSVEVHVLQGEREMAQYNKTLGKFQLTGIAPAPRGMPQIEVSFDIDA
                     "
     gene            complement(1463..2077)
                     /gene="grpE"
                     /locus_tag="PLR57_02795"
     CDS             complement(1463..2077)
                     /gene="grpE"
                     /locus_tag="PLR57_02795"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQQ40411.1"
                     /translation="MTKKNEPIKPAATEEKAADSEQAERKETLTGEPVASKKEAGSDE
                     EVYTLTAEEFAAAKAHIESLQKEKDDTVQLLQHIQADFDNFRKRNASIRLDSYEEGKR
                     DTMKELLPALDSFDRAVSSAGENCDKPLLEGVQLVQRQLLDALKKLGLEEVEASGQFD
                     PNLHEAVLREKAEGKASGDIVEVLQKGYRVGEKVLRHTMVKVAE"
     gene            complement(2136..>3020)
                     /locus_tag="PLR57_02800"
     CDS             complement(2136..>3020)
                     /locus_tag="PLR57_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HQQ40412.1"
                     /translation="DKTLLPTAGSCDKCPKRSGNQAVLFEKADDKDLCTDLACYREGI
                     RAHLTAKLDEVEMVMKQTAQALSAVTHYTAMIMPPMLAANRLRHIQLVPLREGRALMV
                     VVTSAGFAREAVIRVPEEINTVELERISRILTERFRDCRMDQLGERIMKDLGSELFER
                     RAFLNSMVEAIEHKMEPDAPNVQLSGATNMLHFPEYSDVNKAKMFLAAVEGRNTLYEL
                     LKRASRLEFSITIGGENENDMLKDCSVVTATYQIGEEPMGSFGIIGPTRMHYGKVISV
                     LEFMSRCLTEMLTSDDEE"
BASE COUNT          527 a          870 c          871 g          752 t
ORIGIN      
        1 gcgtcgatat cgaacgagac ctcgatctgc ggcatgccgc gcggcgcggg cgcgatgccc
       61 gtgagctgga acttgccgag ggtcttgttg tactgcgcca tttcgcgctc gccctgcaga
      121 acgtggactt ccacgctggt ctggccgtcc gcagccgtgg agaagatttg gctcttcttg
      181 gtcgggatgg tcgtgttgcg gtcgatcagg cgggtgaaca cgccgccgac ggtctcaata
      241 cccagcgaaa gcggggtgac gtccagaagc agaatgtcct tgacttcgcc gccgagcaca
      301 ccgccctgaa tagccgcgcc gatggcgacg cactcatcgg ggttgatgcc cttgaagggg
      361 tctttgccgg taatcttctt gaccatttcc tgcacgacgg ggatacggga agaaccgccg
      421 acgaggatga ccttatccac ctgcgaagcg gagagacccg catcggtcag gcacttctgc
      481 accagagtct tggtctgttc gacgaggtcg ttgatcagct cgttgaactt ggctctggta
      541 atcgtgatgt ccaggtgctt gggaccggat ttatccatcg tgataaacgg caggttgatc
      601 gtcgtctgcg ccacgccgga aagttcgatc ttggcttttt ccgccgcttc cttgaggcgc
      661 tgcagagcca tcttgtcctg acggaggtcg atgccgttgt tgcgcttgaa gtcctcggcg
      721 atgtagtcga tgatcagctg atcaaagtcg tcgccgccca aacggttgtt gcccgcggtg
      781 gcgagaacct ggaagacgcc gtcgccaatt tccagaatgg aaacgtcgaa cgtgccgccg
      841 ccaaggtcat acaccagaat cttctggttg ttttctttat ccataccgta tgccagcgca
      901 gccgccgtcg gctcgttgat gatgcgcagc acttcgagac ccgcaatacg ccccgcgtcc
      961 ttggtggcct ggcgctggga gtctgtaaag taagccggaa cggtaatgac ggcctgcgtc
     1021 accgtggagc cgaggtaggc ttccgcgtcc tgcttgagtt tctgcaggat catggccgaa
     1081 atttcctgcg gcgtgtagtc tttgccttcg atgcggactt tgcgggcgct gcccatgtcg
     1141 cgcttaatcg aagcgaccgt gcggtcgggg ttcgtgattg cctgacgctt tgcaatgttg
     1201 cctaccatgc gttcgccgtc tttgaaagcg accacggaag gcgtggtgcg cgcgccttcg
     1261 gaattcggga tgacgaccgg ttcgccgcct tcgaggaaag cgacgcagga attggtcgtg
     1321 ccaaggtcga tgccaatgat tttgcccata agtgataatc ctccaactgt tctatttaat
     1381 ctatatgaat tcaaatgaat ccggtaataa tgattccgcg gcttaattag ctgtatggat
     1441 ttctgaaaat ccagctaatt aactattccg cgactttaac cattgtgtgc cgcagaacct
     1501 tttcgccgac gcggtagcct ttttggagca cctcgacgat atccccgctg gccttgcctt
     1561 ccgccttttc gcggagaacc gcttcgtgca gattggggtc gaactggccg cttgcttcta
     1621 cctcttcgag gccgagcttt ttaagcgcgt cgagcagctg ccgctgaacg agctgtaccc
     1681 cttcgagcag cggtttgtcg cagttttcgc ccgcgcttga aacggcgcgg tcaaaactgt
     1741 ccagagcggg cagcagttcc ttcatcgtgt cccgcttgcc ctcttcgtaa ctgtccagac
     1801 gaatgctggc gttgcgcttg cggaagttgt cgaaatccgc ctgaatatgc tgcaaaagct
     1861 gaacggtatc gtccttctcc ttctggagag attcgatatg cgcctttgcc gccgcgaact
     1921 cttccgcggt cagggtgtag acttcctcat cggaacccgc ttccttcttt gaagcgacgg
     1981 gttcaccggt aagcgtttcc ttgcgctctg cctgttcgct gtctgccgcc ttctcttcag
     2041 tggcggccgg tttgattggt tcgttcttct tagtcaatgg tcgttctcct gttcctgccc
     2101 gttcggacgc ctgttgagtg gctgtcctgc agcgtttatt cttcgtcgtc actggtcagc
     2161 atttccgtga ggcaccggct catgaattct agaaccgata tgacctttcc gtaatgcatt
     2221 ctcgtcggcc caatgatgcc gaacgagccc atcggttctt cgccgatctg gtaggttgcg
     2281 gtgacgacgc tgcaatcctt gagcatatcg ttttcgtttt ctccgccgat agtgatggaa
     2341 aattccagcc tgctcgcgcg tttgagcagt tcataaaggg tgtttcgccc ttccacggcg
     2401 gcaagaaaca tctttgcttt attgacgtcg ctgtactccg gaaagtggag catattcgtg
     2461 gctcccgaga gctgtacgtt cggcgcgtcc ggttccattt tgtgctcaat ggcttccacc
     2521 atcgaattca aaaacgccct gcgctcaaac aattcgctgc caaggtcttt catgatccgc
     2581 tcgccgagct gatccatgcg gcagtcgcgg aagcgctcgg tcagtatccg cgagatgcgc
     2641 tcgagctcga cggtgttgat ctcctccgga acgcggatga ccgcttcccg cgcaaagccc
     2701 gccgacgtga ccacgaccat cagcgcgcgc ccctcgcgga gcggtacaag ctgaatatgc
     2761 cttagccggt ttgccgcgag cattggcggc atgatcattg ccgtatagtg cgtcaccgcc
     2821 gagagcgcct gtgcggtctg cttcatgacc atttcgactt cgtcgagttt ggccgtcagg
     2881 tgcgcgcgaa tcccctcgcg gtagcaggcg aggtcggtgc agaggtcctt gtcgtcggcc
     2941 ttctcgaaga ggacggcctg gttgccggaa cgcttcgggc acttgtcgca gctgccggcg
     3001 gtcggcagca gggtcttgtc
//
LOCUS       DAOMGY010000127         2996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_35323_length_2996_cov_3.475349, whole genome shotgun sequence.
ACCESSION   DAOMGY010000127 DAOMGY010000000
VERSION     DAOMGY010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35323_length_2996_cov_3.475349"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1315
                     /locus_tag="PLR57_02805"
     CDS             104..1315
                     /locus_tag="PLR57_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HQQ40413.1"
                     /translation="MSNINTTASTAGTTYSNKTFYDRALLEIAKTRLVHANYGQKRSI
                     PRNSGKRVEFRRYELFSPDANALVLEEGVTPAGQSLAQSKIEAEVKQYGAYVEVSDLL
                     DLTSYDPVLGESTELLGEQLGTVVEWVTRDAMCAGTNVQYANGKTNRLALEAADKLTV
                     TEIRKAVRSLKKAKARMFNSTGEGKTRKPHFVCICSPDATFDLQSDSLWQDVSKYADV
                     EQIYSGEIGRLFGVVFVESTEAKVFSQSVLTAVASHSANSAVVTLDEITDAAALYLVS
                     GAKIKIGTTEYTVASSNQTAKTVTLSAAVASAIDADTIVYSEDAGILDASTKKGMDVH
                     ATLVFGADAYGVIDVDGEGCLQTIVKPCGSGGAADPLDQRATVGAKVAAYTARILNNL
                     WIVRIEHGVSL"
     gene            1433..1711
                     /locus_tag="PLR57_02810"
     CDS             1433..1711
                     /locus_tag="PLR57_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40414.1"
                     /translation="MQYITEAEIDNISSDTGKALAAEPKVTIVIHPESGEPYWEGGVN
                     GHFFRIRTNESVAVPQSLATLIAQSAAVKVEIEARTRAFRKSGGKKVS"
     gene            1724..2269
                     /locus_tag="PLR57_02815"
     CDS             1724..2269
                     /locus_tag="PLR57_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40415.1"
                     /translation="MTLKELLTGALLQLDRATDAQTLEAWRDKLTRYLNDAMIDLTSE
                     LQPRRTDELTLTDGTLDLSALPRAVVKVLALTRGSTRLPFYYGLSSDKLRVPAVADGA
                     VSVTYRYIPEPLRVDTDVPDLPEWCHGALIGYAVGRERAAGDAASVNAARACFELYNA
                     AKRCMRAHRGELDAFTIENRG"
     gene            2283..>2996
                     /locus_tag="PLR57_02820"
     CDS             2283..>2996
                     /locus_tag="PLR57_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40416.1"
                     /translation="MSLRQYRIPMFYGLNQGITENRISEGESPDACNMDTSGGRLSVA
                     KGYVRESEVPFSTPEAVQRLFVWNRAEAKRFLVSTADGLFVLDQTAGEWKRLYTFSPQ
                     NSEKRCDFQVLKIASTEYLVVASGACQMAKWDGVSESAAAFGSAEGLSDIAVNFCELY
                     YSRLFAAGDADNPARLYYSQAPGDTRTLENWASAEESENAGGGHVEIGADSDPITGLF
                     ALSNQLLIFKRDSLYRLLGD"
BASE COUNT          681 a          890 c          938 g          487 t
ORIGIN      
        1 ctaccgcggc atggacgcgg aagcgttccg ctcgctgatg tcgcagatga aaaagaccgc
       61 ccgcgacggc ggcaagacca gactgtaacc aacggaggga aacatgagca atatcaatac
      121 gaccgcctcg acggcgggca ccacctacag caacaagacg ttctacgacc gcgcgctgct
      181 cgagattgcc aagacgcgac ttgtgcacgc aaactacggc cagaagcgca gcattccccg
      241 taactccggc aagcgcgtgg agttccgccg ctacgaactc ttttcgccgg acgccaacgc
      301 gctcgtgctg gaagagggcg taacccccgc gggccagagc cttgcccaga gcaagattga
      361 agcggaagtc aagcagtacg gcgcttacgt ggaagtgagc gacctgctcg atctgaccag
      421 ctacgacccc gtcctcggcg aaagcactga actgctcggc gaacagctcg gcaccgttgt
      481 ggaatgggtc acccgcgacg caatgtgcgc gggtacgaac gttcagtacg ccaacggcaa
      541 aacgaaccgg cttgcgctgg aagcggcgga taaactgacc gttacggaaa tccgcaaggc
      601 cgtccgttcg ctcaagaagg cgaaggcgcg catgttcaat tccaccggcg aaggcaaaac
      661 gagaaaaccg cactttgtgt gtatctgctc gccggacgca acgttcgatc tgcagagcga
      721 ctcgctttgg caggacgttt ccaagtacgc ggacgtggag cagatctact ccggcgaaat
      781 cggacggttg ttcggcgtgg tattcgtcga aagcacggaa gccaaggtct tttcgcagag
      841 cgttttgacc gcggtcgctt cccacagcgc caacagcgcg gtggtcacgc tggacgaaat
      901 caccgacgca gcggctctgt atctcgtctc cggcgcaaag atcaagatcg gcacgacgga
      961 atacaccgtt gcgtcctcga accagacggc aaagaccgtc accctctcgg cggcggttgc
     1021 aagcgccatc gacgcggaca cgatcgttta cagcgaggac gcgggcattc tggacgcttc
     1081 cactaaaaag ggcatggacg tccatgcgac gctggtcttc ggcgcggacg cgtacggcgt
     1141 gatcgacgtg gacggcgaag gatgcctgca gaccatcgtc aagccctgcg gcagcggcgg
     1201 cgcggcggat ccgctcgacc agcgcgcaac ggtaggcgcg aaagtcgccg cctatacggc
     1261 caggattctc aacaatctct ggatcgtgcg cattgagcac ggcgtgagcc tgtaaggtca
     1321 ggcagtaacg gcgcgggagg gcggaaacgc tctcccgcaa agtaaatgag tgcaagaaaa
     1381 atcaggataa gaaaaatcag gataagaaaa atcagatttg gaggaatcaa acatgcagta
     1441 cattacggaa gccgaaatcg acaatatttc gtccgatacc ggaaaagcgc ttgccgcgga
     1501 accgaaggtt accatcgtca tccacccgga gagcggcgaa ccctactggg aaggcggcgt
     1561 gaacggacat tttttccgca tccgcaccaa cgagagcgtg gccgttcccc agagccttgc
     1621 cacgctgatt gcccagagcg ccgccgtaaa ggtggaaatc gaggcgagaa cccgcgcctt
     1681 ccgcaagagc ggcggcaaaa aggtttcgta agggggatgc gttatgacgc tcaaggaact
     1741 gctcaccggc gcgctgctcc aactcgaccg cgcgaccgac gcgcagacgc tggaggcatg
     1801 gcgggacaag ctcacgcggt atctcaacga cgcgatgatc gacctcacca gcgaactgca
     1861 gccgcgccgg acggacgaac tgacgctgac ggacggcacg ctcgatctga gcgcgctgcc
     1921 gagagcggtg gtcaaggtgc tcgcactcac gcgcggcagc acgcgtctgc ccttctatta
     1981 cgggctctca agcgacaagc tgcgcgtgcc cgccgtggcg gacggagctg tatcggtgac
     2041 gtaccgctac attcccgaac cgcttcgggt ggatacggat gttcccgatc tgccggaatg
     2101 gtgccacggg gcgctgatcg gctacgcggt cggacgcgaa cgcgctgccg gcgacgccgc
     2161 gagcgtgaac gccgcgcgcg cctgcttcga actctacaac gcggcaaaac gctgcatgcg
     2221 cgcgcaccgc ggcgaactgg acgcgtttac catcgaaaac cgcggataag gagggaatgc
     2281 atatgtcgct caggcaatac cggatcccga tgttctatgg cctgaatcag ggcataacgg
     2341 aaaaccgcat cagcgaaggc gaaagcccgg acgcctgcaa tatggacacc tccggcgggc
     2401 ggctcagcgt cgccaaaggg tatgtgcgcg aaagcgaggt tccgttttcc acgccagaag
     2461 ccgtgcagcg gctgtttgtc tggaaccgcg cggaagcaaa acgctttctc gtttccacgg
     2521 cggacgggct gttcgtttta gatcagacgg cgggggaatg gaagcggttg tacaccttca
     2581 gcccgcagaa cagcgaaaag cgctgcgact ttcaggtgct gaaaatcgcg agtacggaat
     2641 acctagtcgt tgccagcggc gcttgccaga tggcaaagtg ggacggcgtt tcggaatctg
     2701 cggccgcgtt cggcagcgcg gaaggcctct cggacatagc ggtgaatttc tgcgaattgt
     2761 attattcccg cctgtttgcc gcgggggacg cggacaatcc cgcaaggctg tattactcgc
     2821 aggcgccggg cgacacgcgc acgctggaaa actgggcgag cgcggaggaa agcgaaaacg
     2881 caggcggcgg gcacgtggaa atcggcgcgg actccgaccc gattaccggc ctgttcgcgc
     2941 tcagtaacca gcttttgatc ttcaagcgtg actcgcttta ccgcctgctg ggcgat
//
LOCUS       DAOMGY010000128         2993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_35366_length_2993_cov_3.170865, whole genome shotgun sequence.
ACCESSION   DAOMGY010000128 DAOMGY010000000
VERSION     DAOMGY010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35366_length_2993_cov_3.170865"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /gene="xseA"
                     /locus_tag="PLR57_02825"
     CDS             complement(<1..639)
                     /gene="xseA"
                     /locus_tag="PLR57_02825"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQQ40417.1"
                     /translation="MEQTLTPVFSVSELNEYVATLLGADPNLCDIRVSGEISGFKRHT
                     SGHLYFSLKDENALVRCVMFRQQAMRLNFNPQDGMRVLLLGRASLYERDGTFQLYANY
                     LKKSGEGELFAKFLALKAELEQKGYFAQERKRPIPFLPKRVGVVTSETGAAVQDIVNI
                     IRRRFPRMPIVLAPVRVQGQGASAEIARAIQLMNEANAADVLIVGRGGGSLED"
     gene            complement(671..1534)
                     /locus_tag="PLR57_02830"
     CDS             complement(671..1534)
                     /locus_tag="PLR57_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HQQ40418.1"
                     /translation="MLSVPLGDRGLRQSDGVFQHVRNLPPLIDALLSNIDRTAVRAVA
                     VSAKPTADEDSYMPVFLAGKLAAVSVAGALGVPLLETTHQAGHIRAAMLDNEYAVPTD
                     KPFLAMHLSGGTTDLLSVRLENGTVGAIERIGGCDDLHAGQFVDRVGVRLGLPFPSGS
                     ALEQLARAAKLRQIRLPSSVKGLTCSFSGQESQAQRLIEQGEAPEEVAYAVYDCMART
                     FSKMLLNAMEQTGCQTALLSGGVSGSELLRELLAERMKKPLLFAGKGRSSDNAIGTAL
                     LARDRFMNGEI"
     gene            complement(1624..2043)
                     /gene="nusB"
                     /locus_tag="PLR57_02835"
     CDS             complement(1624..2043)
                     /gene="nusB"
                     /locus_tag="PLR57_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQQ40419.1"
                     /translation="MSRTSAREVAMMMEFSRMLGGEDTPERVCDKAENIGEGALDAED
                     VLYVEQINGGVNAHQQEIDTLISKYSVDWAIERIARVDLSILRVAIYEMLYRKEDVPA
                     GAAINEAVELAKRYGGERSYAFINGILGSVAKELAST"
     gene            complement(2139..2369)
                     /locus_tag="PLR57_02840"
     CDS             complement(2139..2369)
                     /locus_tag="PLR57_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HQQ40420.1"
                     /translation="MNGSFLEFIKEHKFTIILVLIGLILSILFFTIGFWKTILLMLIL
                     ALCFFVGFLLDKGGLEGLKDFFNKLFSNNKTV"
     gene            complement(2381..>2993)
                     /gene="amaP"
                     /locus_tag="PLR57_02845"
     CDS             complement(2381..>2993)
                     /gene="amaP"
                     /locus_tag="PLR57_02845"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HQQ40421.1"
                     /translation="RRALRRADEKGVIAMKLKWFDRLLLGILLIVAIVASFVLFGMAA
                     NLIQEPVVTDFIALFYMYKVNALILAGSGLVLLLISIKLLFAGKGKKTEVRPASALMK
                     QTEFGGTFITLEALDAMIQKHCRAQQRVKDCHTTLHSSEEGVTVGIRLAVLPDTDVVS
                     LSSELQKTLKEYVEGLTGIHVKEIGILVENAAAPATASVARID"
BASE COUNT          633 a          919 c          796 g          645 t
ORIGIN      
        1 gtcctcgaga gagccgccgc cgcggcctac gatcagaacg tccgcggcgt tcgcttcgtt
       61 catcagctga atcgcgcgcg caatctccgc cgatgcgccc tgcccctgca cccgcactgg
      121 agcgagaacg atgggcatgc gcggaaaacg cctgcggatg atgttgacga tatcctgcac
      181 cgccgcgccc gtttcgctgg tgacaacgcc gacgcgcttt ggtaaaaatg gaatcggccg
      241 cttgcgctcc tgcgcaaaat agcccttttg ctccagttcg gccttgagcg ctaaaaactt
      301 tgcaaacaac tcgccttcgc cgctcttttt tagataattg gcgtagagct gaaacgttcc
      361 gtcccgttcg taaagggaag ctctacctag aagcaacacg cgcatgccgt cctgcgggtt
      421 gaagttcagg cgcatggcct gctggcggaa catcacgcag cgcacgagcg cgttttcgtc
      481 cttgagtgaa aagtacaggt gcccggacgt atgccgtttg aagccgctga tttcaccgct
      541 gacgcggata tcgcagaggt tcgggtctgc gcccagcaag gtggcgacgt attcgttcag
      601 ttcgctgacg ctgaataccg gtgtaagcgt ctgctccatt ttatcgcccc ctcccgccga
      661 tttatgtgcg ttaaatttcg ccgttcatga acctgtcccg cgcaagcaga gccgttccga
      721 tggcattgtc gctggaacgc cctttgcccg caaacagcag cggttttttc atccgctctg
      781 caagcagttc gcgcagcagc tcgctgccgg aaacgccgcc ggaaagcaac gcggtctggc
      841 aacccgtctg ttccatggcg tttaatagca tcttggagaa tgtccgcgcc atgcagtcgt
      901 ataccgcgta ggcgacttcc tccggcgctt cgccctgctc gatcaaacgc tgggcttgcg
      961 attcctgccc ggaaaacgaa catgtcaaac cctttacgct cgaaggcagg cgaatctgcc
     1021 gcagtttcgc cgcgcgggcg agctgctcca gcgcgctccc gctcggaaac ggcagtccca
     1081 gcctgacccc gacgcgatct acgaactgac cggcgtgcag atcatcgcag ccgccgattc
     1141 gctcaatcgc gccgacggta ccgttctcca gccggacgga gagcaagtcc gtcgtcccgc
     1201 cggaaaggtg catagccaaa aacggcttgt ccgttggaac ggcatattcg ttatccagca
     1261 tcgcggcgcg gatatgcccg gcctgatgcg tcgtttccag caacggaacg cccaaagcgc
     1321 ctgcaacgga caccgccgca agtttacccg cgagaaacac cggcatgtag gagtcctcgt
     1381 ccgcagtcgg ctttgcgctg accgcaacgg cgcggaccgc ggttcggtcg atattgctta
     1441 agagcgcatc gatcagcggc ggaaggttac ggacgtgctg aaacaccccg tcggactgcc
     1501 gtaagccccg gtcgcccaac ggcacagaaa gcatcgttct ccgttcgaaa acgatgcctt
     1561 cctcggatac gcagcaaacc gacgtcgtat aacagcttgt atcgacgcct aaaaagagac
     1621 ggctcatgtg gaagcgagtt cttttgcgac gctgcccaga atgccgttga taaacgcata
     1681 ggagcgttcg ccgccatagc gtttggccag ttccaccgct tcgttgatgg ccgcgcccgc
     1741 gggcacgtcc tctttgcggt aaagcatttc atagatcgcc acgcgaagta tgctcaaatc
     1801 cactctagcg atgcgctcga ttgcccagtc cacggagtat tttgagatca gcgtgtcgat
     1861 ctcctgctga tgcgcgttca cgccgccgtt gatctgctca acgtaaagaa cgtcctccgc
     1921 gtccagcgcg ccttcgccga tgttttcggc tttgtcgcaa acgcgttcgg gcgtatcctc
     1981 tccgccaagc atgcgggaaa attccatcat catcgcgact tcacgcgcgc tcgttctgct
     2041 catgcaatac tttccttacc caattgtctg gacactccgg ctgcgccgcg attcagaccg
     2101 ttttattagt tgtctagacg ctccggctgc gccgcgcctc agaccgtttt attattcgaa
     2161 aacagtttgt tgaaaaaatc tttcagccct tcaagcccgc ccttgtcgag taaaaagcca
     2221 acaaagaagc agagcgcgag gataagcatc agaagaatgg tcttccaaaa cccgatggtg
     2281 aaaaacagga ttgaaagaat caacccgatc agcaccagga tgatggtgaa tttatgttcc
     2341 ttgataaact cgagaaacga gccgttcatg atgcccctcc tcagtcgatc cgcgcaacgc
     2401 tcgccgtagc aggcgccgcc gcgttttcaa ccagaatgcc gatttctttg acatggatgc
     2461 ccgtcaggcc ttccacatac tctttcagtg ttttctgcag ctcgcttgaa agcgaaacaa
     2521 cgtccgtatc cggcagtacc gcgaggcgga tacctaccgt gacgccctct tccgaagagt
     2581 gcagcgttgt gtggcagtcc ttgacgcgct gctgtgcgcg gcagtgcttt tgaatcatgg
     2641 cgtccagcgc ttccagcgta atgaacgtgc cgccaaactc cgtctgcttc atcagcgcgg
     2701 aagccgggcg aacttcggtc tttttgcctt tacctgcaaa cagcagcttg atcgagatga
     2761 gcagaagcac aagcccgctg cctgcgagaa tcagcgcgtt caccttatac atgtagaaca
     2821 gcgcgatgaa gtccgttacg acaggctctt gaatgaggtt tgccgccatg ccgaagagca
     2881 caaacgacgc tacgatggct acgattaaga gaatgcccag caacagccgg tcgaaccatt
     2941 tcagtttcat tgcgataacc cctttttcat cggcgcgacg gagtgcccgt ctg
//
LOCUS       DAOMGY010000129         2992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_35379_length_2992_cov_3.439224, whole genome shotgun sequence.
ACCESSION   DAOMGY010000129 DAOMGY010000000
VERSION     DAOMGY010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35379_length_2992_cov_3.439224"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /gene="clpX"
                     /locus_tag="PLR57_02850"
     CDS             complement(<1..858)
                     /gene="clpX"
                     /locus_tag="PLR57_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HQQ40422.1"
                     /translation="MAKNEEPSVVRCSFCGKAQEDVHRIIAGPGVNICNECVELCREI
                     IEEDAEAEPIDLSNIPKPQEILTQLNQYVIGQDMAKRALAVAVYNHYKRINSPAVKGD
                     DVELQKSNIIMLGPTGCGKTMLAQTLARILNVPFAVADATSLTEAGYVGEDVENILLK
                     LIQAADYDVEKASKGIIYIDEIDKIARKGENVSITRDVSGEGVQQALLKILEGTVASV
                     PPQGGRKHPHQEFIQIDTTNILFICGGAFSGVDKIIEKRIGHKILGFGAGVQSNVDKD
                     VGEVLKHILP"
     gene            complement(881..1465)
                     /gene="clpP"
                     /locus_tag="PLR57_02855"
     CDS             complement(881..1465)
                     /gene="clpP"
                     /locus_tag="PLR57_02855"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HQQ40423.1"
                     /translation="MNLIPMVIEQTSHGERSYDIYSRLLKDRIIFIGGPIDDDTANIV
                     VAQMLFLEGDDPDKDINLYINSPGGSVSAGLAIYDTMQYIKCEVATICIGLAASMGAF
                     LLAAGAKGKRKALPNAEIMIHQVSGGAQGQATDINIQAEQILKIKRRLNEILAARTGQ
                     TIERVTQDTERDNYMSAEEACAYGLIDEVFPPRR"
     gene            complement(1550..2947)
                     /gene="tig"
                     /locus_tag="PLR57_02860"
     CDS             complement(1550..2947)
                     /gene="tig"
                     /locus_tag="PLR57_02860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQQ40424.1"
                     /translation="MATVEKLEKNRAKLTVTVTPEAFAAAIQQAYFKTAKRYNIPGFR
                     KGKAPKKVIENMYGEGVFYEDAFELVWGDAYDAALAEHSLTAVDKPSLDISSISLAEG
                     IVFTAEVQLKPEVSLGAYKKLEVPEPEFTVDDSEVLAELEKEREKSARFIEVERAVEN
                     GDRVVLDYSGSVDGEKFEGGTAEDQLLVIGSGTFIPGFEEQLVGMKTGEEKDISVRFP
                     DEYSAPLAGKDAVFSVKIKAVQIKELPALDDEFAKDISDFDTLEALKADKKAKMLEKA
                     EKNRKTAIENVALKIASDNAIVEIPDVMVERQINYMLRDIAYQLSMSGLSLDDYCKYT
                     GTDLNGLKESYREEALARVKMQLVIEAIGEKEGVYCTEEDKKAAIAEYAEGSGKTPEE
                     FEKTLTADDMEYIEDHVVAQKTVKIITDSVVLVKGEAEKKPAKKAAAKKAEAGAEAEA
                     KPAKKPAAKKAEPKE"
BASE COUNT          477 a          869 c          865 g          781 t
ORIGIN      
        1 cggcaggatg tgcttgagca cttcgccgac gtccttatcc acgttgctct gcacgcccgc
       61 gccaaagccg aggattttat gcccgatgcg cttttcgatg atcttgtcca cgcccgaaaa
      121 cgcgccgccg cagataaaca ggatgttcgt ggtatcgatc tggatgaact cctgatgcgg
      181 atgcttgcgc ccgccctgcg gcggaacgga agcgaccgtg ccttcaagga ttttcagcag
      241 cgcctgctgc acgccttcgc cggaaacgtc ccgcgtgatg gagacgtttt cgcccttgcg
      301 cgcgatcttg tcgatctcgt cgatgtagat aatgcccttg ctggccttct ccacgtcgta
      361 atcggcggcc tgaatgagct tgagcaggat gttttccaca tcctcgccga catacccggc
      421 ctcggtgagg ctggttgcgt ccgcaacggc gaacggtacg ttgagaatcc gcgccagcgt
      481 ctgcgccagc atggtcttgc cgcagccggt cggcccgagc atgatgatgt tgctcttttg
      541 cagctccacg tcgtcgccct tgacggcagg cgagttgatg cgtttgtaat ggttatacac
      601 cgcaacagcc agcgcgcgct tggccatatc ctggccgatg acgtactggt tgagttgggt
      661 taaaatctcc tgcggtttgg ggatgttgct caggtcgatg ggttcggcct cggcgtcctc
      721 ctcaatgatt tcccggcaga gctcaacgca ttcgttgcag atgttgacgc ccggccccgc
      781 gatgatgcgg tgcacgtcct cctgcgcttt gccgcagaac gagcagcgca cgacggacgg
      841 ttcttcgttt ttcgccatag tgtacctcta aaattcaggg ttatctgcgc ggcgggaaga
      901 cctcgtcgat cagcccgtac gcgcacgcct cctcggcgct catgtagttg tcgcgctcgg
      961 tatcctgcgt gacccgttcg atcgtctgcc ccgtgcgcgc ggcaagaatt tcgttgagac
     1021 ggcgcttgat tttgagaatt tgttcggcct ggatgttgat atccgtcgcc tgtccctgcg
     1081 cgccgccgct gacctgatgg atcatgatct cggcgttggg cagcgctttg cgcttgcctt
     1141 ttgcgccggc cgcgagcaga aacgcgccca tggaggccgc aaggccaatg cagatggtgg
     1201 cgacttcgca cttgatgtac tgcatcgtgt cgtaaatggc aagccccgcg ctgaccgagc
     1261 cgccggggga gttgatgtag aggttgatgt ccttatcggg gtcgtcgccc tctaaaaaga
     1321 gcatctgcgc cacgacgata tttgccgtgt cgtcgtcgat gggcccgccg ataaagatga
     1381 tgcggtcctt gagcaggcgg ctgtagatat cgtagctgcg ttcgccgtgg ctggtctgct
     1441 cgatgaccat cggtataagg ttcatgttcg ttccctcctt tggggaaatg taggggttgc
     1501 ggtttcgccg cgtttgatcc gtttggctga aacgctgcgc gaaaatgagt tactctttcg
     1561 gctccgcctt cttggccgcg ggcttcttgg cgggttttgc ttccgcttcc gcgcctgctt
     1621 ccgctttctt ggcggcggct ttcttggcgg gcttcttctc ggcttcgccc tttacgagca
     1681 ccacgctgtc ggtgatgatc tttacggttt tctgcgcgac tacgtggtct tcgatatatt
     1741 ccatatcgtc ggcggtgagc gtcttttcga attcttccgg cgttttaccg ctgccttcgg
     1801 cgtattccgc gatggcggct ttcttgtcct cttccgtgca gtagacgcct tccttttcgc
     1861 cgatggcttc gatgacgagc tgcatcttga cgcgcgcgag cgcttcttcg cggtagcttt
     1921 ctttcaggcc gttgaggtcg gtgccggtat acttgcagta gtcgtccagc gagagcccgc
     1981 tcatgctgag ctgatacgcg atgtcgcgca gcatgtagtt gatctgccgc tcgaccatga
     2041 cgtccggaat ctcgacgatg gcgttgtcgc tggcaatctt cagcgcgacg ttttcgattg
     2101 cggtcttgcg gttcttctcc gctttttcga gcatcttggc cttcttatcg gctttgagcg
     2161 cttcgagcgt gtcgaagtcc gaaatatcct tggcaaactc gtcgtccagc gcgggcagtt
     2221 ccttgatctg aacagccttg atcttgacgg agaacacggc gtccttgccg gccagcggcg
     2281 cggagtattc gtccgggaag cggacggaga tatccttttc ctcgccggtc ttcatgccga
     2341 cgagctgttc ttcaaagccc ggaataaacg tgccggagcc gatgaccaga agctgatctt
     2401 ccgcggtgcc gccctcgaat ttttcgccgt ccacgctgcc ggagtagtcc agcaccacgc
     2461 ggtcgccgtt ttccacggcg cgctcgactt cgataaagcg ggcgctcttt tcgcgctctt
     2521 tttcgagttc ggccagcact tcggaatcat cgacggtgaa ttccggttcg ggaacttcca
     2581 acttcttgta agcacccaga gaaacttccg gctttaactg cacttcggcg gtaaagacga
     2641 tgccttccgc gaggctgatg gagctgatgt cgagggaggg tttatccact gcggtcagac
     2701 tgtgctcggc aagcgccgcg tcgtacgcgt cgccccagac gagttcgaac gcgtcctcat
     2761 agaaaacgcc ttcgccgtac atgttttcaa tgaccttctt gggcgctttg cccttgcgga
     2821 aacccggaat attgtaacgc ttcgcggttt taaaataagc ctgctgaatc gcggcggcga
     2881 atgcttcggg ggtgacggtt acggtcagtt tcgcgcggtt tttctccagt ttctctacgg
     2941 ttgccatttg ctcctccatt ttttcggcat ctattctcaa tagccatata at
//
LOCUS       DAOMGY010000130         2978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_35614_length_2978_cov_2.740677, whole genome shotgun sequence.
ACCESSION   DAOMGY010000130 DAOMGY010000000
VERSION     DAOMGY010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35614_length_2978_cov_2.740677"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLR57_02865"
     CDS             <1..453
                     /locus_tag="PLR57_02865"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HQQ40425.1"
                     /translation="FYRTYGKPEEKNGTIRVTSFTFWRRHKKSKDWLIIGGVLLLAAV
                     VFFVSRLMKPDISGAAVTVYVGGEVYARVSANEYQTITIDQGDGKVNVVVIDAAGVRM
                     DSSTCKNQLCVKHAPVDPNNIGEIEFDNWIICMPNGVSVEVTGGEGGQ"
     gene            450..1031
                     /locus_tag="PLR57_02870"
     CDS             450..1031
                     /locus_tag="PLR57_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HQQ40426.1"
                     /translation="MSKRPMGAGTIARLGLLTAVALVLGYVEHLIPVTAIPGIKLGLA
                     NTVLLYALYLLDVPSAILLMILKVGLGGLFYGGFAAMLYSLAGGVLSLFVMILVKKAR
                     GVSVIGASVCGAAAHNIGQILIACFVVGVRAALAYLPVLLVAAAITGALTGIIAKYVF
                     RTFSPKSAGACTASEPAKANGAARQGENTEQHP"
     gene            1227..2153
                     /locus_tag="PLR57_02875"
     CDS             1227..2153
                     /locus_tag="PLR57_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQQ40427.1"
                     /translation="MTTVIVYDTSEKNAQGVLTRANELLLEYHKLYDIYHAYDGMNNL
                     YTVNQSAGGAPVTVDARIIDLLKFAKEMDVLTNGGTNVAMGCVTELWRVCREAGVEDP
                     AHAALPAEEELTRAARHTNIEDVLIDESANTVQLADPEMLLDVGAIAKGYAVQQVLDQ
                     LKSEGVTSLLLSAGGNVAAIGTRPDGTSWKVGIQDPYSGGYLCAVNVDGMCLVTSGTY
                     ERYYTVDGKRYHHIIDPNTLYPSAYYDSVSVLCADSGLADALTTGLFSMPLEQGQALV
                     ASLDGVEAFWILPDGTQVMSAGFGAFIIQTTD"
     gene            2221..>2978
                     /locus_tag="PLR57_02880"
     CDS             2221..>2978
                     /locus_tag="PLR57_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HQQ40428.1"
                     /translation="MYNTRKVNEDITWVGGDDRRLAMFEGVYSIPQGVSYNSYLVMDE
                     QTILFDTVDKAIGKVFFENVSHVLGERKLDYVVVQHMEPDHSATLMDLLCHYPEVKVV
                     CNQKTRDMIAQFFGEDLGERAHLVKEGDTLSTGRHEFTFVFAPMVHWPEVMFTYDKTD
                     RVLFSADAFGTFGALGGALFADEMDFYNECLPEARRYYTNIIGKYGLQVQSALKKACA
                     LDVTVICPLHGPVHRKDFGAYIDKYRQWSSYTPE"
BASE COUNT          651 a          815 c          895 g          617 t
ORIGIN      
        1 ttttatagaa cgtatggcaa gccggaggag aaaaatggta caatacgagt aacaagtttc
       61 actttttgga ggcggcacaa gaagagcaag gactggctga tcatcggcgg agtactgctg
      121 ctggctgccg tcgtcttttt cgtttcgcgc ctcatgaagc cggatatttc gggtgccgcg
      181 gtgaccgtat atgtcggcgg cgaggtgtac gcgcgcgttt ccgcaaacga ataccagacc
      241 atcacgatcg atcagggcga cggcaaggtg aacgtcgtcg tcatcgacgc agccggagtg
      301 cggatggatt cctccacctg caaaaaccag ctctgcgtaa agcatgcgcc ggtcgatccg
      361 aacaacattg gcgaaatcga attcgacaac tggattatct gcatgccgaa cggcgtatcg
      421 gtggaagtca ccggcgggga gggcggacaa tgagcaaacg acctatgggc gcgggaacca
      481 tcgctcggct cggccttctc acggcggttg cgctcgtgct tggttatgtg gaacacctga
      541 ttcccgtaac ggcgattccc ggcatcaagc tggggcttgc caatacggtg ctgctctatg
      601 cgctgtacct gctggacgta cccagcgcaa tcctcttgat gattctcaaa gtaggtctcg
      661 gcggcctgtt ttacggcggg tttgcggcaa tgctctatag ccttgcgggc ggagtgctga
      721 gcctttttgt gatgatcctc gtcaaaaaag cgcggggggt cagcgtcatc ggcgcgagcg
      781 tctgcggagc tgcggcgcat aacatcggcc agattctcat tgcgtgcttt gtggtaggcg
      841 tccgcgcggc gcttgcgtac ctgccggtgt tgctggttgc ggcggcgatc accggcgcgc
      901 tcacgggtat aatcgccaaa tacgtctttc gtacgttctc gccaaagtcc gccggcgcgt
      961 gcaccgcatc cgaacccgcc aaagcgaacg gcgccgcacg gcagggcgaa aatacggaac
     1021 aacacccgta agagtcttga tgaaacacaa aaaacatgga ataatgccaa cagcgactgt
     1081 tcattcaata agggggaacc taagcacgaa gcgttgcatt tgcccttgcg gttctgctga
     1141 ccgccagcct gctcctgacg gggtgtggcg cgagaaccgc ggaaccggca aggtacgaaa
     1201 agactttttt tgacgcgttc gacaccgtga caacggtgat tgtttacgat acgagcgaaa
     1261 agaacgccca gggcgtttta acccgcgcga acgaactcct gctggagtac cataaactct
     1321 acgatatcta ccatgcgtac gacggcatga acaacctcta taccgtcaac cagagcgcgg
     1381 ggggcgcgcc ggttacagtt gacgcacgga ttatcgattt gctgaagttc gccaaagaga
     1441 tggatgttct cacaaacggc ggaacgaacg tcgccatggg ctgcgtgacc gaactctggc
     1501 gcgtctgccg tgaagctggt gtggaagacc cggcgcacgc cgcgcttcca gcggaagagg
     1561 aactgacccg cgccgcccgg cacacgaata ttgaggacgt gctcattgac gaatccgcaa
     1621 acacggttca gctggccgac ccggagatgc tgctggacgt tggcgcaatt gcaaaagggt
     1681 acgccgtcca gcaggtgcta gatcagctca aaagcgaagg cgttacgtcg cttttactca
     1741 gcgcgggcgg caacgtggcc gccatcggca cgcgtccgga cgggacaagc tggaaggtcg
     1801 gcattcagga tccgtattcg ggcgggtacc tctgcgcggt caacgtggac ggcatgtgtc
     1861 tcgtgaccag cggcacctat gagcgctact acaccgtgga cggcaagcgc tatcaccaca
     1921 ttatcgaccc gaacacgctg tatccttccg cgtattacga ctccgtttcc gtgctttgcg
     1981 cggactctgg cttggcggac gcgctgacca cggggctgtt cagcatgccg ctggaacaag
     2041 ggcaggcgct cgttgcttcg ctcgacggcg tggaagcctt ctggattctg ccggacggca
     2101 cgcaggtgat gagcgcgggg ttcggcgcat ttatcatcca aacgactgat taatcgaggg
     2161 gttcttcccc tctggaataa aggtttttta cgcaacatat ttggaaaggg gcatacggcc
     2221 atgtacaaca caagaaaagt aaacgaggac atcacctggg taggcggcga cgaccggcgg
     2281 ctcgccatgt ttgaaggcgt gtattcgatt ccgcagggcg tttcctacaa ctcttatctg
     2341 gtcatggacg aacagaccat tctgttcgat acggtcgata aggccatcgg caaggtgttt
     2401 tttgaaaatg tttcccatgt gctcggcgag cgcaagctgg actacgtcgt ggtgcagcat
     2461 atggagcccg accactcggc aacacttatg gatctattgt gccattatcc ggaagtcaag
     2521 gtcgtttgca accagaagac cagggatatg atcgcccagt tctttggtga ggaccttggc
     2581 gaacgtgcgc acctcgtgaa ggaaggcgac accctctcga ccggcaggca cgagtttacg
     2641 tttgtgttcg cgcccatggt gcactggccg gaagtcatgt tcacctacga caaaaccgac
     2701 cgcgtgctgt tttccgcgga cgcgttcggc acgttcgggg cgttgggcgg cgcgctgttt
     2761 gccgacgaaa tggactttta caacgaatgc ctgccggaag cgcggcggta ttacaccaac
     2821 attatcggta aatacggcct gcaggtacag tccgcgctca agaaagcctg cgcgctggac
     2881 gttacagtca tctgcccgct gcacggacct gtgcaccgca aggattttgg cgcatatatc
     2941 gataagtacc gccagtggag cagctatacg ccggagga
//
LOCUS       DAOMGY010000131         2971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_35731_length_2971_cov_3.938615, whole genome shotgun sequence.
ACCESSION   DAOMGY010000131 DAOMGY010000000
VERSION     DAOMGY010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35731_length_2971_cov_3.938615"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..496
                     /gene="rpsG"
                     /locus_tag="PLR57_02885"
     CDS             26..496
                     /gene="rpsG"
                     /locus_tag="PLR57_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HQQ40429.1"
                     /translation="MPRRGFIPKREVLADPIYNSVVVTKLINQVMLDGKRGVSQKAVY
                     DAFDIIREKTGREPGEVFEQAMNNIMPVLEVKARRVGGATYQVPIEIRTERRQTLGLR
                     WLVNFARKRSERTMSARLAGEIMDGANSTGNAFKKKEDTHKMAEANKAFAHYRW"
     gene            727..2808
                     /gene="fusA"
                     /locus_tag="PLR57_02890"
     CDS             727..2808
                     /gene="fusA"
                     /locus_tag="PLR57_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQQ40430.1"
                     /translation="MPRDTALEKTRNIGIMAHIDAGKTTTSERILFYTGKIHKIGEVH
                     EGAAVMDYMVQEQERGITITSAATTAYWRAHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VAVFCAKGGVEPQSETVWRQAVKYGVPCIAYVNKMDITGADFNRVCDMMRDRLGANAL
                     PIQLPIGSEDTFRGIIDLVTMKAEIYYSDDGTDIRQEEIPADMLKTAKEQRAKLLDTV
                     IEEDEHLMERYFNGEWFTAEELIPCIRKATLSGRVVPVTCGSSYRNKGVQPLLDAIVD
                     YLPSPLDVPPIKGTSKDGEKEMAVEPRDDAPFAALALKILTDPFVGKLAFIRVYSGTL
                     KSGSYVYNVSKNKRERVGRILMMHADSRTEIEEAHAGDIIALVGLKDTTTGDTLCVES
                     HQLLLERMEFPDPVIQVAIEPKTKAGQEKMGLALVKLAEEDPTFRTYTDQETGQTIIA
                     GMGELHLEIIVDRMIREFHVEARVGNPQVSYKESIKAPVKAEARYVRQTGGHGQYGHV
                     VIELAPREPGAGYEFIDKIVGGVIPREYIPAVDKGIREALKSGHLGGYEVVDVQATLL
                     DGSYHEVDSSELAFTIAGSLAVKEALEKANDVLLEPMMKVEVLVPEDYLGDVMGNLTA
                     RRGKVQGMESRQNVQVIDAVCPLSEMFGYATDLRSRTQGRGTFTMQFSHYDEVPRSIA
                     ESVVGKNNYRL"
     gene            2841..>2971
                     /locus_tag="PLR57_02895"
     CDS             2841..>2971
                     /locus_tag="PLR57_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQQ40431.1"
                     /translation="MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITMALAQQGEAKA
                     "
BASE COUNT          716 a          852 c          891 g          512 t
ORIGIN      
        1 gaagaagtaa gggagggccg aacaaatgcc aagaagagga tttattccca agcgcgaagt
       61 gttggctgac ccgatttaca acagcgttgt ggtcaccaaa ctcatcaacc aggtcatgct
      121 ggacggcaaa cgcggcgtat cccagaaagc ggtttacgat gcgtttgaca tcatccgtga
      181 aaagaccggc cgggaacccg gcgaagtgtt cgagcaggcc atgaacaaca tcatgcccgt
      241 tctcgaagtc aaggcacgca gagtcggcgg cgccacctat caggtgccga tcgaaatccg
      301 caccgagcgc cgtcagacgc ttggccttcg ctggctcgtc aactttgcgc gcaagcgcag
      361 tgaacgcacc atgagcgcgc gcctcgcggg cgaaatcatg gacggcgcca actccacggg
      421 caacgccttc aaaaagaagg aagacaccca caagatggcc gaagcgaaca aggcgttcgc
      481 gcactatcgc tggtaaatcc gcgcgaacaa cggtttgcgc gcgggcagag cgatttgcca
      541 tgcgccgccg ttttgcacgg tttggaacgt gttgtcccgg gggaaacgca ctggaaacgg
      601 gctttccttt cgggagcaaa atgaggagtc aacagccacg ggcctgcggt tcggagcagt
      661 caatccgaac cgcagacccg cgcggactgt tagggaacaa tattcgacaa gcagagggaa
      721 acgattttgc cgagagatac cgcactggaa aaaacaagaa atattggcat catggcgcat
      781 attgacgccg gaaaaaccac cacgtccgaa cgaattctgt tctacacggg caagattcac
      841 aagatcggcg aggtgcacga aggcgcagcc gtcatggatt acatggtgca ggagcaggag
      901 cgcggaatta ccattacgtc cgcggccact accgcttact ggcgcgcgca ccgcatcaac
      961 attatcgaca cgccgggtca cgtggacttc accgtggaag tggagcgttc gctccgcgtt
     1021 ctcgacggcg cggtcgctgt tttttgcgcc aaaggcggcg tagagccgca gagcgaaacc
     1081 gtctggcgtc aggccgtcaa atacggcgta ccctgtattg cttacgtcaa taaaatggac
     1141 attaccggcg cggacttcaa ccgcgtctgc gatatgatgc gcgaccgcct cggcgccaac
     1201 gcgctgccca tccagctgcc catcggcagc gaggatacgt tccgcggcat catcgacctc
     1261 gtcaccatga aggcggagat ttattacagc gacgacggaa cggatatccg gcaggaagaa
     1321 attcccgcgg atatgctcaa aaccgccaag gagcagcgcg caaagcttct ggatactgtc
     1381 atcgaagagg acgagcatct gatggagcgg tatttcaacg gcgagtggtt caccgctgaa
     1441 gagctcattc cctgcatccg caaggcgacg ctctccgggc gtgtggttcc cgtcacctgc
     1501 ggctcatctt accgcaacaa gggcgtgcag ccgctgctcg acgcgattgt ggactacctg
     1561 ccgagccccc tcgacgttcc cccgatcaag ggtacgagta aggacggcga aaaggaaatg
     1621 gcagtggaac cgcgcgacga cgcgccgttt gccgcgctcg cgctcaaaat cctgaccgac
     1681 ccgtttgtcg gcaagctcgc gttcatccgc gtttacagcg gcacgctcaa gtccggctcc
     1741 tacgtgtata acgtaagcaa gaacaagcgc gaacgcgtcg gccgcatcct gatgatgcac
     1801 gccgattccc gtacggagat tgaggaagcg cacgcgggcg atatcattgc attggtcggc
     1861 ctgaaggata cgaccaccgg cgacacgctc tgcgtggaaa gccatcagct cctcttggag
     1921 cgcatggagt tcccggatcc cgtcattcag gtcgccatcg agcccaagac caaagcaggc
     1981 caggagaaga tgggccttgc gctggtcaag ctcgcggagg aagaccccac gttccggacg
     2041 tataccgatc aggaaacggg tcaaaccatc atcgcgggca tgggcgaact gcatctcgag
     2101 atcatcgtgg atcgcatgat ccgcgagttc catgtggaag cacgcgtggg caacccgcag
     2161 gtttcctata aagaatcgat caaagcgccc gtcaaagcgg aagcgcgcta cgtccgccag
     2221 accggcggcc acggtcagta tggccatgtg gttatcgagc ttgcgccgcg cgaacccgga
     2281 gcgggctatg aattcatcga caagatcgtt ggcggcgtaa ttccgcgcga atatatcccc
     2341 gccgtcgata agggcatccg cgaagcgctc aagagcggcc atctcggcgg ctatgaggtt
     2401 gtggatgtgc aggcaacgct gctcgacggc tcctaccacg aggttgactc ctccgagttg
     2461 gcgtttacca tcgcgggttc cctggcagtc aaggaagcgc tggagaaggc gaacgacgtg
     2521 ctgctcgaac ccatgatgaa ggttgaagtg ctcgtgccgg aagactacct tggcgacgtg
     2581 atgggcaacc tcaccgcacg ccgtggaaaa gtgcagggca tggagtcgcg ccagaacgtt
     2641 caggtgatcg acgcggtctg cccgctttcc gaaatgttcg ggtatgcaac cgaccttcgc
     2701 agccggacgc aggggcgcgg aacgttcacc atgcagtttt cgcattatga cgaagtaccg
     2761 agatcgatcg ccgagtcggt ggtcggaaaa aataattacc gtttataaca aacgattgat
     2821 cagaaaagag gagagctaac atggcaaagg caaaattcga gagaacgaaa ccgcacgtca
     2881 acatcgggac gatcgggcac gtagaccacg gcaagacgac gctgacggcg gccatcacga
     2941 tggcgctggc gcagcagggc gaagccaagg c
//
LOCUS       DAOMGY010000132         2967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_35809_length_2967_cov_2.554258, whole genome shotgun sequence.
ACCESSION   DAOMGY010000132 DAOMGY010000000
VERSION     DAOMGY010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35809_length_2967_cov_2.554258"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="PLR57_02900"
     CDS             complement(<1..862)
                     /locus_tag="PLR57_02900"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQQ40432.1"
                     /translation="MRTADFISDAATLGSDDARQTPELTRYALEGFAIEVDAKIQSEL
                     LTKLILSHSEIARRLETAVETLRRSEAALKEAQQIALLGRWDIDLATGRRVWSESLYS
                     ILELDPSTEPTAELFLSRLHPDQREEALRIYAALSERKEPWTIHYRLLMPDGREKVIQ
                     LLVHTEYAEGKAVRSYGTLQDVTAVAKAEEELENYNKHLQQMVADKVQELTQAQMATI
                     YALVKLSESRDDDTGAHIERTASFCRLLAEKARSHPAYAGQIDDEFLDSIFKASPLHD
                     IGKVGISDTIL"
     gene            complement(859..1683)
                     /locus_tag="PLR57_02905"
     CDS             complement(859..1683)
                     /locus_tag="PLR57_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ40433.1"
                     /translation="MQKTTENLDLDPIEIAEGIYWLGYTNNNAGLHCNPYLIREGDEA
                     VLIDGGNRDDFSAVMLKILRTGLQPAQIKLLIYQHYEPDLCGNLPQLEALINTKELRI
                     ISHAENNVFIHYYSTKTPKLDYRVLQSEFLFATGRRLTFHGTPFCHSPGSFMTYDERT
                     KTLFSSDLFGSYDAQWDLFFAFQEGCSECSHTRICPINGKHCPTYGIEQFHTRIMTSN
                     RALSMALDVVESLDTERIAPQHGSILTNREDVKRIVGFLRALNSVGIDAVAAEQTP"
     gene            complement(1863..2759)
                     /locus_tag="PLR57_02910"
     CDS             complement(1863..2759)
                     /locus_tag="PLR57_02910"
                     /inference="COORDINATES: protein motif:HMM:NF040016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HQQ40434.1"
                     /translation="MNELTPIVSPLPEGLREPRGEAFAAEFITLLAERTARYTMGDST
                     SVRLETAERLAGGILYCIELAQNSRSPGAAGSLKALYETGVREAERIKRHGKLLLRQA
                     ESIRPPVENIGFRDTLKALPAFFRAYDTAFFAQEIPGDIDYPLCHPVSESLKGAAYVC
                     EYLRRLNLETAFLRRFSAPLLTLVYERIYLGDGGLLVNLYMPAAEAALGRALAGKTVR
                     NLLTDASDRALIRLKMNNLTEPAAKRILQQAANRVGVELGMTGGLEREYLVQTAEALL
                     PRLLALEAEPGYRGIFASREAG"
     gene            complement(2752..>2967)
                     /locus_tag="PLR57_02915"
     CDS             complement(2752..>2967)
                     /locus_tag="PLR57_02915"
                     /inference="COORDINATES: protein motif:HMM:NF040063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6323 family protein"
                     /protein_id="HQQ40435.1"
                     /translation="TLCALTEAFYSFKNDCFDVLTDDELIACLRACYDAYEGGLDAVC
                     SLTIEELARGRRLDDADDPDDGEEADE"
BASE COUNT          622 a          879 c          813 g          653 t
ORIGIN      
        1 acaaaattgt atcggaaatg cccactttac cgatgtcgtg cagagggctc gccttaaaga
       61 tggagtccag gaactcatcg tcgatctgac cggcgtaggc gggatgactg cgggctttct
      121 ccgcaagcaa acggcaaaag ctcgccgtgc gctcaatatg cgcaccggta tcgtcgtccc
      181 gcgattcgga gagctttaca agcgcgtaga tcgtcgccat ctgcgcctgg gtcagttcct
      241 gcactttgtc cgccaccatc tgctggaggt gcttgttgta attttccagc tcttcttccg
      301 ccttggccac cgcggtcacg tcctgcaagg tgccgtaact gcgaaccgct ttgccctctg
      361 catactcggt atgcacaagc agctgaatca ccttttcgcg tccgtccggc atcagcagtc
      421 ggtagtgtat tgtccacggc tcttttctct ccgataaagc agcgtatatc ctcagcgctt
      481 cttcccgctg atcgggatgt aagcgggaaa gaaacagttc tgccgtcggt tccgtcgacg
      541 ggtcgagctc caaaatgctg tagagggatt cggaccagac gcgcctgcca gtggcaaggt
      601 caatatccca gcggcccagc aaagcaatct gctgcgcttc cttcagcgcg gcttcactgc
      661 ggcgcagagt ttccaccgct gtctcgagcc gacgggcaat ttcggaatgg ctcaaaatca
      721 gtttggtgag aagctcgctc tgaatttttg catcgacttc tatggcaaac ccctccagcg
      781 cgtagcgcgt cagttcgggg gtttgccgcg catcgtcact gccgagcgtc gcagcgtccg
      841 aaataaaatc cgcagttctc atggcgtttg ctccgcggcg actgcgtcga ttccaacgct
      901 attgagcgcg cgcagaaagc cgacgatccg ttttacgtcc tcgcggtttg taagaatact
      961 gccatgctgc ggagcgattc gttccgtatc gagggattcc acaacgtcca gcgccatgga
     1021 gagcgctctg ttggacgtca tgatgcgggt atgaaactgt tcgatgccgt atgtcgggca
     1081 atgtttgccg ttgatcgggc aaatccttgt gtgcgagcac tcgctgcacc cttcctgaaa
     1141 cgcaaagaac aaatcccact gcgcatcgta actgccgaac aggtcgctgg aaaagagcgt
     1201 ctttgtccgt tcatcatagg tcataaagct acccggcgaa tgacagaacg gcgtgccgtg
     1261 aaacgtaagc cgccgcccgg tggcgaacaa gaattctgac tgcagtacgc gataatcaag
     1321 ctttggcgtt ttggtggagt agtagtgaat aaaaacgttg ttttccgcat gcgatatgat
     1381 gcgcagttcc tttgtgttga tcagcgcttc cagctgcgga agattgccgc aaaggtcggg
     1441 ttcgtagtgc tggtagatca ggagtttgat ctgcgcgggc tgcaggccgg tgcgcaaaat
     1501 tttgagcatc acggcgctga agtcatcccg gtttccgccg tctatgagca ccgcctcgtc
     1561 cccttcccga atcaagtacg ggttgcagtg caggcccgcg ttattgttcg tatacccgag
     1621 ccagtagatc ccttccgcga tttcgatggg gtccagatcc agattctcgg tcgtcttctg
     1681 catatcgttc cctccgttct cccatggact cactgttaat agaatagcag aaatgcagtt
     1741 caggcgcttt tgatagctcc caaacagaaa gcgactacct gaaaaacggg aaacaaaaat
     1801 cagttatacc tgttaattcc attttacaaa gtcaaatccg ataatgcaat cgaaaaaaag
     1861 gtttatccag cctccctgga ggcgaaaatc ccccgatacc ccggctcggc ttccagcgcg
     1921 agcagccgcg ggaggagagc ttcggcggtt tgaaccagat attcccgttc cagtccgcct
     1981 gtcatgccca gctccacgcc gacgcggttt gccgcctgct gcagaatccg ttttgcggcg
     2041 ggttcggtaa gattgttcat cttcagccgg atcaacgcgc ggtcggaagc gtccgttagc
     2101 aagtttcgca cggtttttcc cgcgagcgcg cgccccagcg cagcctcggc agcgggcatg
     2161 tacaggttga ccaaaagccc gccgtcgcca aggtaaatcc gctcgtaaac cagcgtaaga
     2221 agcggcgcgg aaaagcgccg gagaaacgcc gtttcaagat tgagcctgcg aagatattcg
     2281 cagacatacg ccgcgccctt cagcgattcc gaaactggat ggcagagcgg atagtcgata
     2341 tcgccgggaa tttcctgcgc aaaaaacgcc gtgtcgtagg cgcgaaaaaa agcgggcagc
     2401 gccttcagcg tatcccgaaa accgatgttc tccaccggcg gacggatcga ctcagcctgc
     2461 cgcagcagca gctttccgtg ccgtttgatg cgctccgcct cccgaacgcc cgtctcgtac
     2521 agcgccttca gtgaacctgc cgcgccggga gagcgcgaat tctgtgccaa ctcaatgcag
     2581 tagaggattc ctcccgcgag ccgttccgcc gtttccagcc gaacggaggt gctgtccccc
     2641 atcgtatacc gggcggtgcg ctccgccagc agagtaataa attccgcggc aaacgcttcc
     2701 ccgcgcggtt cacgcaaacc ttccggcagc ggcgaaacga tcggcgtcag ttcattcatc
     2761 cgcttcctcc ccgtcgtcag gatcgtccgc gtcgtccagc cgccgtccgc gcgccagttc
     2821 ttctatcgtg agcgagcaga ccgcgtccag cccgccctcg taagcgtcgt agcaggcgcg
     2881 aaggcaggcg atcagttcgt cgtccgtgag cacgtcgaag cagtcgttct tgaatgaata
     2941 aaacgcttcc gtcagcgcgc agagcgt
//
LOCUS       DAOMGY010000133         2960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_35931_length_2960_cov_3.552496, whole genome shotgun sequence.
ACCESSION   DAOMGY010000133 DAOMGY010000000
VERSION     DAOMGY010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35931_length_2960_cov_3.552496"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="PLR57_02920"
     CDS             complement(<1..1662)
                     /locus_tag="PLR57_02920"
                     /inference="COORDINATES: protein motif:HMM:NF023324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3416 domain-containing protein"
                     /protein_id="HQQ40436.1"
                     /translation="MRNKRFFAALCALALLLTILPASTLAEESDSWWQGDIWDTAALT
                     ALGALPPAYNADTQQYEISTPEQLLYLSGTWKPEDTNADGAPDAPCDGVYVLTADLDM
                     QPLMERIGQALTSLSGTAAEGYMPPIAALTDEGRDGGVNCAFFGTFDGQGHVISNLRI
                     ERMQSKYAGLFGNVGHDLGEGTVRNLALFNIEIKCLASGGLLVGGLYGDVENCVVIGS
                     IDCAEKTAGGIAGKVKKNDNGYLGTVSNCFVYADILVRGEGDENGAAGGVTSAQSDGG
                     RIYNCIVGGSITVLGEKADSVGGITGNLKSGQALENTLMCLKKIDVNDGANIGLLCGD
                     YAGETGSHLLNNFVWNGTVLSGGVSSDHPETAAFTSVDAAAIQTKSFYADTLGWDFEA
                     LWTWIGEDDSGYPMLSQFTGTGGVLENLNAAFSSNLAVTEPILRLSEPMTTKAYAGDE
                     VPVTCTLVLPEGVTASLATLRYGTEKDASAFTDSVPMADNGDSTFTAVFPLTEAGEYY
                     YSVEAVAGGKTLLFPNQAGASVRLDLVSPEAKYQPRQLTVSPGADV"
     gene            complement(1751..2761)
                     /locus_tag="PLR57_02925"
     CDS             complement(1751..2761)
                     /locus_tag="PLR57_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40437.1"
                     /translation="MKKLLAVLLSLTMIFIAVACTATPAATEPAEEATVTEAPAVEAT
                     EAPAETEAPAEEPAVVTYVPLGLDFKNKTGVTITGLYLYPTGSEDKGMSICSAEWVDK
                     DNDPNEEFYEFQAYIVRPASETYDVFVEFADGTNATWTGVAVQTNDKLSLKDGVDPST
                     WELEALEDQEDLDKVAAVIAAGKTSDNTYPGYETLGLEIKNKTGKGITEFYFYETGGD
                     YTMYKNMVASLVDDTGAAVTVWQSGKGGLYVFNFFLRPHADTYEVYVVYEDGTTMTVP
                     AIDLFTLNGDGFASNEISMKDAVDPDLTEVSYDDGDPEPLQLIKDAIAAGIPADAWYP
                     VY"
BASE COUNT          550 a          842 c          897 g          671 t
ORIGIN      
        1 aacatccgcg ccgggggaga cggtcagttg cctgggctgg tatttcgctt ccggcgagac
       61 cagatcaagg cgaacgcttg cgcctgcctg attgggaaac agcagcgttt tgccgcctgc
      121 aacagcttca acgctgtagt agtattcgcc tgcttccgtc agcgggaata ccgcggtaaa
      181 cgtactgtcg ccgttgtcgg ccatgggtac ggaatccgta aacgcgctcg cgtccttttc
      241 cgtgccgtag cgcagcgtgg ctaaagaagc agtcacgcct tccggcagga caagcgtgca
      301 agtaaccggc acttcgtcac ccgcgtaggc cttggtggtc ataggttcgg acaagcgcag
      361 aattggctca gtaactgcca aattgctgct gaaagccgcg ttcaggtttt ccagcacgcc
      421 accggttcct gtgaactgac taagcatcgg gtagccggaa tcgtcctcgc cgatccacgt
      481 ccagagcgct tcaaaatccc agccgagcgt atctgcgtag aaggattttg tctgaatcgc
      541 ggctgcgtca accgatgtaa acgcggcggt ttcgggatgg tcgctggaca cgccgccgct
      601 gagaacggtg ccgttccaga caaagttgtt caaaaggtgc gaaccggttt ctcccgcgta
      661 gtcgccgcag agcaggccga tattcgcgcc gtcgtttacg tctatctttt tcaggcacat
      721 cagcgtattt tccagcgcct gaccggactt gaggttgccc gtaattccgc ctacgctgtc
      781 ggccttttcg ccgagcacgg tgatgctgcc gccgacgatg cagttgtaga tgcgcccgcc
      841 gtcggactgc gcgctggtca cgccgcccgc tgcgccgttt tcatcgcctt cgccgcgcac
      901 gaggatatcc gcgtagacaa agcagttgga aacggtgccg aggtagccgt tgtcgttctt
      961 tttcaccttg ccggcgatgc cgcccgcggt cttttccgcg cagtcgatcg agccgatgac
     1021 gacgcagttt tctacgtccc cgtacagacc gccgacaagc aagccgccgg aagcgaggca
     1081 cttgatttct atattgaaaa gcgccagatt gcggacggta ccttcgccga ggtcgtgacc
     1141 cacgttgccg aagagcccgg cgtacttact ctgcatccgc tcgatgcgga ggttggagat
     1201 tacgtgaccc tgaccgtcga acgtgccgaa aaacgcgcag ttcacgccgc cgtcccggcc
     1261 ttcgtccgtc agcgcggcaa tcggcggcat atagccttcc gcggcagtgc cggagaggct
     1321 ggtcagcgcc tgcccgatgc gttccatcag cggctgcata tccaaatccg cagtcagcac
     1381 atacacgccg tcacagggcg cgtccggggc gccgtcggca ttcgtatcct ccggcttcca
     1441 cgttccgctg agatagagca gctgctccgg cgtggagatt tcgtattgtt gcgtatctgc
     1501 attataggcg ggcggcagcg cgcccagcgc tgtcagcgcg gcggtatccc agatatcgcc
     1561 ctgccaccag ctgtcgcttt cttccgcaag agtgcttgcg ggtaatatgg tcaataacaa
     1621 cgccagcgcg cagagcgctg cgaagaatct tttattgcgc atggagaaat cctcccttga
     1681 aaatgatccg aaacaggcgg gggatcacag tattgtgatc cccctggcat tttagtttag
     1741 attgttttga ttagtacacc gggtaccatg cgtcggccgg aatgccggcg gcgatggcgt
     1801 ccttgatgag ctggagcggt tcgggatcgc cgtcgtcata ggagacttcc gtcagatccg
     1861 gatccactgc gtccttcatg gagatttcgt tgctggcaaa tccgtcgcca ttcagcgtga
     1921 agaggtcgat tgcgggaacc gtcatcgttg tgccgtcttc ataaacgaca tagacttcgt
     1981 aggtatccgc gtgcggacgg aggaagaagt tgaacacata caggccgccc ttgccggact
     2041 gccagacggt gactgccgcg ccggtatcgt ccacgaggct tgcaaccatg ttcttgtaca
     2101 tggtgtagtc tccgccggtt tcatagaaat agaactccgt gatgcctttg ccggtcttgt
     2161 tcttgatttc caggccgagg gtttcgtaac ccggataggt gttgtcggac gtcttgcccg
     2221 cggcgatgac ggcggcaact ttatcaaggt cttcctgatc ttccagcgct tcgagttccc
     2281 aggtggaagg atcaacgccg tccttgaggg agagcttgtc gttggtctga acggcaacgc
     2341 cggtccaggt ggcgttcgtg ccgtccgcaa actctacaaa cacgtcgtag gtttcggaag
     2401 cggggcggac gatgtacgcc tggaattcat agaactcttc gttcgggtcg ttgtccttgt
     2461 caacccattc ggcggagcag atgctcatgc ctttgtcttc cgatccggtc gggtagagat
     2521 acaggccggt aatcgttacg cccgtcttgt ttttgaagtc gagtccgagc ggcacatagg
     2581 tgaccactgc gggttcttcg gccggcgctt cggtttcggc gggggcttcc gtcgcttcaa
     2641 cggcgggagc ttcggtgacg gtggcttctt cggcgggctc cgtggcggcg ggcgtcgccg
     2701 tgcaggcaac cgcgatgaag atcatcgtca gggacaacag aactgctagt agttttttca
     2761 tgatcttacc tcctgtttgc ttttatgggg agccgcacac cgtgatgcgg cagtggtacg
     2821 ggggataggc tgcggatatc cgaggagcgg acgaacgctg ctacactcga tgatggattg
     2881 aaccgtcggg atggatttcc agccggacgg caatctgggt cgaggcgggg taatgatgaa
     2941 aagcgcggtt ttccgtgcag
//
LOCUS       DAOMGY010000134         2960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_35938_length_2960_cov_2.602065, whole genome shotgun sequence.
ACCESSION   DAOMGY010000134 DAOMGY010000000
VERSION     DAOMGY010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35938_length_2960_cov_2.602065"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="PLR57_02930"
     CDS             <1..1297
                     /locus_tag="PLR57_02930"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQQ40438.1"
                     /translation="KDIDSSWEAVKYAKHPRIHVFIATSDIHMEYKLKKTREEVIEQT
                     GEMVRYAKKYCEDIEFSAEDATRSDMDFLCRVFETAIEAGATVLNIPDTVGYTTPEEF
                     AYLIGYIREHTKGVENVQLSVHCHNDLGMAVANSLAAIRAGVDQVECTVNGIGERAGN
                     AALEEIAMNLRTRADYYGASCGIDTTQIYKSSRLLSSITGVKVQPNKAIVGENAFAHE
                     SGIHQHGMLANKQTYEIMDPASIGLAQNKMVLGKHSGRHAFNERVHDLGFTVDRELSN
                     MLFKRFKDLADKKKTVSDRDIEALIRSILAQEIPKSYALERYVINSGNSITPTSSIVL
                     VKSTGERTEKVAIGFGPVDASFNAINKIVRMQIRLEEYSIGAVTEGRDAQGEVSVKIS
                     SKGATAKGYGLSTDVIEASILAYVNAINNLIYGSAAEQE"
     gene            1299..2558
                     /gene="leuC"
                     /locus_tag="PLR57_02935"
     CDS             1299..2558
                     /gene="leuC"
                     /locus_tag="PLR57_02935"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQQ40439.1"
                     /translation="MGMTITQKILASHAGQDLVTAGQLIRAKVDLVLGNDITSPVAIA
                     EFEKAGIERVFDREKIALVMDHFTPNKDIKAAEQCKTVRAFARKHDIKYFFDVGRVGV
                     EHALLPELGLVKPGDLVIGADSHTCTYGALTAFSTGVGSTDMAAGMATGYCWLKVPSA
                     IRFVLKGELRPWVSGKDVILYIIGKTGVDGALYQSMEFTGPGLKNLTMEDRLCMANMA
                     IEAGAKNGIFEYDAETARFATERGCKNWTIYTADEDAEYTATYEIDLCEIPPTVAFPH
                     LPDNARAVADCGDVTIDQAVIGSCTNGRIEDLRAAAKVFEGRHVHENVRCIVIPATQK
                     IMLQAMEEGLVKTFIDAGCAVSTPTCGPCLGGHMGILAAGERAVATTNRNFVGRMGHA
                     GSEVYLASPAVAAASAILGRLADPGQI"
     gene            2572..>2960
                     /locus_tag="PLR57_02940"
     CDS             2572..>2960
                     /locus_tag="PLR57_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQQ40440.1"
                     /translation="MNAAGTAIKYGDNIDTDVIIPARYLTTTNADELAAHCMEDIDKT
                     FLQRVKPGDIMVGGKNFGCGSSREHAPIAIKASGISCVIAATFARIFYRNAINIGLAI
                     IECPEASERIQEGDEVSVDFDTGVITNL"
BASE COUNT          701 a          868 c          869 g          522 t
ORIGIN      
        1 aaaggatatc gacagttcgt gggaagcggt gaagtacgca aagcacccgc gcatccatgt
       61 gtttatcgcc acatccgata tccatatgga atacaagctc aaaaagacgc gcgaggaagt
      121 cattgaacag accggcgaga tggtgcggta cgcaaaaaaa tactgcgagg atatcgagtt
      181 ctccgccgag gacgcaacca gaagcgatat ggactttctc tgccgcgtgt ttgaaaccgc
      241 catcgaagcg ggcgcaaccg tgctcaacat tccggatacg gtcggctaca ccacgccgga
      301 ggagttcgcc tacttaatcg gctatatccg cgagcacact aaaggcgtgg aaaacgttca
      361 gctttccgtt cactgccaca acgatttagg catggcggtc gccaactcgc tcgccgccat
      421 cagggcgggc gtagatcagg tagagtgcac ggtcaacggc atcggcgagc gcgcgggaaa
      481 cgccgcgctg gaggaaatcg ccatgaacct gagaacccgc gcggactact acggcgcgtc
      541 ctgcggcatc gacaccacgc agatttacaa gagcagccgc ctgctttcca gcatcaccgg
      601 cgtaaaagtg cagcccaaca aagccatcgt cggcgaaaat gcgtttgccc acgagtccgg
      661 cattcaccag cacggcatgc tcgccaacaa gcagacctac gagattatgg atcctgcttc
      721 gatcggactt gcgcagaaca aaatggtgct gggcaagcac tccggcaggc acgcgttcaa
      781 cgagcgcgtt cacgatttgg gctttacggt agaccgcgag ctttccaaca tgctcttcaa
      841 gcgctttaag gacctcgcgg acaagaagaa aaccgtgagc gaccgcgata tagaggcgct
      901 catccgcagc atcctcgcgc aggagattcc caagtcgtat gcgctggaac ggtacgtcat
      961 caactccggc aactcgatta cgccaacttc cagcatcgtg ctggtcaagt ccaccggtga
     1021 gcggacggag aaggtcgcca tcggctttgg ccccgtggac gcttcgttca acgcgatcaa
     1081 caagatcgtc cgcatgcaga ttcgtttgga ggaatactcg atcggcgcgg tcacggaagg
     1141 acgggacgcg cagggcgagg tcagcgttaa aatctccagc aagggagcaa cggcgaaagg
     1201 ctacggcttg agtacggacg taattgaggc cagcattctg gcctacgtca acgccatcaa
     1261 caacttaatc tacggcagcg cagcggagca ggaataatat gggaatgacg ataacgcaga
     1321 aaatactcgc gtctcacgcg gggcaggatc tcgtaacggc gggtcagctg atccgcgcga
     1381 aggtcgatct tgtgctgggc aacgacatca cctcccccgt tgcgattgcg gagtttgaaa
     1441 aagcgggcat cgagcgcgtg ttcgaccgcg aaaaaattgc gctcgtgatg gatcacttta
     1501 cgccgaataa agatatcaaa gccgccgaac agtgcaaaac cgtccgcgcg tttgccagaa
     1561 agcacgacat caagtacttt ttcgacgtag ggcgcgtcgg cgtggagcac gcgctgctgc
     1621 cggaactggg tctggtcaag ccgggcgatc tggtcatcgg cgcggactcg cacacctgca
     1681 cctacggcgc gctgaccgcg ttctccacgg gcgtcggcag cacggacatg gcggcaggca
     1741 tggcgacggg gtactgctgg ctgaaggtgc cttccgcaat ccgctttgtg ctcaaaggcg
     1801 aactgcgccc ctgggtttcc ggcaaggacg ttatcctcta catcatcggc aaaacaggcg
     1861 tggacggcgc gctgtaccag tccatggagt tcaccggccc gggcttaaag aacctcacga
     1921 tggaagaccg tctctgcatg gcgaacatgg caatcgaggc gggcgcgaag aacggcattt
     1981 tcgaatacga cgcggaaacc gcgcgctttg ccaccgagcg cggctgcaaa aactggacga
     2041 tctataccgc ggacgaggac gcggagtaca ccgcgacgta cgaaatcgac ctctgcgaaa
     2101 ttccgcccac cgtcgctttt cctcacctgc cggacaacgc aagagccgtt gcggactgcg
     2161 gagacgtaac cattgatcag gcggtaatcg gctcctgcac gaacgggcgc atagaagatt
     2221 tgcgcgcggc ggcaaaggtg tttgagggca ggcacgtaca cgaaaacgtc cgctgcatcg
     2281 tcatccccgc cacgcagaag atcatgctgc aggcgatgga ggaaggcctt gtaaaaacct
     2341 ttatcgacgc gggctgcgcg gtatccaccc ccacctgcgg cccctgcctc ggcgggcaca
     2401 tgggcattct ggcagccggg gaacgcgcgg tggcaacgac caaccgtaat ttcgtagggc
     2461 gcatggggca cgcggggtcg gaagtgtatc ttgcttcccc cgccgtagcc gccgcatccg
     2521 cgatcctggg caggctggcc gaccccggac agatatagga ggagaagcga aatgaacgca
     2581 gcaggaaccg ccatcaagta tggcgacaat atcgatacgg atgtaatcat ccccgcgcgg
     2641 tacttaacca ccacaaacgc cgacgaactc gccgcgcact gcatggagga tatcgacaaa
     2701 acctttttgc agcgcgtaaa gccgggcgac atcatggtcg gcggcaagaa ctttggctgc
     2761 ggctcttccc gcgagcatgc gccgattgcc attaaggctt ccggcatctc gtgcgtaatt
     2821 gcggcaactt ttgcgcgtat cttctaccgc aacgccatca acataggcct cgctatcatc
     2881 gaatgccccg aggcttcgga gcggattcag gaaggcgacg aggtctccgt cgatttcgac
     2941 acgggcgtta tcaccaacct
//
LOCUS       DAOMGY010000135         2950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_36116_length_2950_cov_2.998618, whole genome shotgun sequence.
ACCESSION   DAOMGY010000135 DAOMGY010000000
VERSION     DAOMGY010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36116_length_2950_cov_2.998618"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1319)
                     /locus_tag="PLR57_02945"
     CDS             complement(9..1319)
                     /locus_tag="PLR57_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQQ40441.1"
                     /translation="MKLQIKKAVALLLVVALLVGTVTMLSGCGKKADANKLTLWINGK
                     DSYIGPSEQKLSQDQWYISQAIQRFEDANPGVTVELVVSADGETAHQTFKADAMAGTA
                     PDLANLWTGQNIFAMEDVMMDISKLIPADDLANISGWDSVTSPATGAILGYPTSDNQI
                     CFFIYNKQIIADCGLDFDNNPPRTIEDFNAACQTILDKGYQPIASDESFPWMGCYVVA
                     YWWVQQTGMDGILSSCKGETKFVDDQGLLSALDYYRSTIVNGYLNADAVTSADSWNKF
                     CQGTVAMMPQVSSVVSDAEAALGAENVGVMLPPDFAGATITNKTIGGPGQCIVVSKDC
                     DNPELAVKFLSFLNSRDEVLKFLEVQSKVPTRLDITAADLNLAEGSAAAKLFGWADSI
                     VFWVDNSLYSTVVGDFYNLLPLVLAGKMTPAEFAADLDSKVVAG"
     gene            2103..>2950
                     /locus_tag="PLR57_02950"
     CDS             2103..>2950
                     /locus_tag="PLR57_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQQ40442.1"
                     /translation="MLDKTSAVPLYAQLAERIKAQILSGEIKPGDKLKSEAEMVEEYD
                     VGRLTVRSALSQLVEEGYLEKAQGKGTFCRAAAEVNYLNVEAVLDMGNTYFIPTYYVK
                     GISEVLNTNNCNFLISDTNSDAAYLCAQLEQILTKNTSGVIFQLTQVQLSPEIRRRLF
                     ACLTAFRNRGIPCIMIDSRIDDAEASYVMLDETAGGARVAEHFAAFGHKRCAVAYAPN
                     CCDALQRYDGFCAAAAEYGMETPVAIDCDAGMEGALIKAVREQGVTGVFGYNDEIALR
                     CMRALRA"
BASE COUNT          633 a          766 c          941 g          610 t
ORIGIN      
        1 taaaggactt agcctgcgac aaccttgctg tcgaggtcgg ctgcgaattc cgcgggggtc
       61 atcttgcctg cgagcacgag gggcagcagg ttatagaaat cgccgaccac ggtggaatac
      121 agcgagttgt ccacccagaa cacgatgctg tccgcccaac cgaagagctt ggcggcggcg
      181 gaaccttccg cgaggttcag gtccgcggcg gtgatgtcca gacgggtggg aaccttgctc
      241 tgaacctcaa ggaacttgag cacttcgtcc ctggagttca ggaaggagag gaacttcacg
      301 gcgagttcgg ggttatcgca gtccttggag acgacgatgc actggcccgg gccgccgatg
      361 gtcttgttgg tgatggttgc gcccgcaaag tccggcggca gcataacgcc gacgttctcc
      421 gcgccgaggg cggcttccgc atcggaaacg acgctggaaa cctgcggcat catggcgacg
      481 gtgccctggc agaacttatt ccaggaatcc gccgaggtga cggcgtccgc gttgaggtaa
      541 ccgttgacga tggtcgagcg gtagtagtcc aacgcgctga gcagaccctg atcgtcgacg
      601 aacttggttt cgcccttgca gctgcttaaa atgccgtcca taccggtctg ctgcacccac
      661 cagtaagcca cgacgtagca gcccatccac gggaagcttt cgtcggacgc gatgggctga
      721 tagcctttgt ccagaatggt ctggcaggcg gcgttgaaat cttcaatggt gcgcggcggg
      781 ttgttgtcga aatccagacc gcagtccgcg atgatctgct tgttgtagat gaagaagcaa
      841 atctggttgt cgctggtcgg atagccgagg attgcgccgg tggcggggga agtgacggaa
      901 tcccacccgg agatgttcgc gaggtcatcc gcggggatca gcttggagat gtccatcatg
      961 acgtcttcca tggcgaagat gttctggccg gtccaaaggt ttgcgaggtc cggcgcggtg
     1021 cccgccatcg cgtccgcttt gaaggtctga tgcgcggttt cgccgtcggc ggagaccacg
     1081 agctcaaccg ttacgccggg gttggcgtct tcaaagcgct ggatggcttg agagatgtac
     1141 cactgatcct gcgagagctt ctgctcgctg gggccaatgt agctgtcctt accgttgatc
     1201 cagagcgtca gcttgttggc gtcggctttt ttgccgcagc cggagagcat ggtgacggta
     1261 ccaaccagaa gggcaacaac gagaagcaga gcaacggcct ttttgatttg taacttcatt
     1321 ctgttccatc ctttacactc gtatttgaat agggaggtga acccctgatt ccggatttag
     1381 cgggcagcga ggccggggag cgagcggcgc ggcagggcgc gcgcactcgt atgggtgaat
     1441 cgggaggtga gcccctgatt ccgggttaag cggccagcga ggccggaagg gcgagggcgg
     1501 cgcggcaagg cacgcgcact cgtatgggtg aatagggagg tgagcccctg attccgggtt
     1561 aagcggtcag cgaggccgga aaggcgagga cggcgcggca gggcgcgcgc actcgtatgg
     1621 gtgaataggg aggtgaaccc ctgattccgg gttgagcggc cgagccgttt atgcgccctc
     1681 ggcgcggcag ggcgcgcgca ctcgtatggg tgaataggga ggtgagcccc tgattccgaa
     1741 ttgagcggcc gagccgtttg cgcgcccgcg gcgcgaaaga gtacaacaag taaggttact
     1801 tccgatagtc tgatccagtg gaaagcttta ggttgtttgc gttgtgcggc agcacggatg
     1861 aaaaatacga aactatttgc caacttatct gcagatatgt tataataact acgaaaggtg
     1921 cttcgcgcac cttacaaaac ttatcctgcc acgagtttgg cgaaatgtca atagttattg
     1981 aaaaaatgtt atgattgaca acaacatcac actgttgtta aatggtagat atcaggagat
     2041 gctgttagga tatctgctag aaacgaatat gttatctgca acttatctgg aggtagccgc
     2101 ctatgctcga taaaacaagc gctgttccgc tctatgccca gctggcggag cggattaaag
     2161 cgcagatcct atcgggagaa atcaagccgg gagacaaact gaaaagcgaa gccgagatgg
     2221 tggaggaata cgacgttggc cgcctgacgg ttcgcagcgc gctctcccag ctcgttgaag
     2281 aaggctatct cgaaaaagcg cagggcaaag gcaccttctg tcgcgcggcg gcggaggtga
     2341 attacctcaa tgtggaagcg gttcttgaca tgggcaatac ctactttatt ccgacctatt
     2401 acgtaaaagg catctccgaa gtgctcaaca ccaacaactg caatttctta atcagcgata
     2461 cgaattccga tgcggcgtac ctttgcgccc agctcgaaca gatactcaca aagaatacct
     2521 ccggcgtgat ttttcagctc acgcaggttc agctttctcc ggagatccgg cggaggttgt
     2581 ttgcgtgcct gacggcgttt cgcaaccgag gcattccctg tatcatgatc gacagccgca
     2641 ttgacgacgc ggaagcaagc tacgtcatgc tggacgaaac cgcgggcggc gcaagggttg
     2701 ccgagcactt tgcggcgttt ggccacaagc gctgtgcggt cgcttacgcg ccgaactgct
     2761 gcgacgcgct ccagcgctac gacggttttt gcgcggcggc ggcggaatac ggaatggaaa
     2821 ctcccgtcgc gatcgactgc gacgcgggca tggaaggcgc tttgatcaag gccgtgcgcg
     2881 agcagggcgt aacaggggta tttggttaca acgacgaaat cgcgctgcgc tgcatgcgtg
     2941 cgctgcgggc
//
LOCUS       DAOMGY010000136         2948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_36149_length_2948_cov_3.543381, whole genome shotgun sequence.
ACCESSION   DAOMGY010000136 DAOMGY010000000
VERSION     DAOMGY010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36149_length_2948_cov_3.543381"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..643
                     /locus_tag="PLR57_02955"
     CDS             230..643
                     /locus_tag="PLR57_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40443.1"
                     /translation="MRKQKDSEYQGFIAISYSEEAKPILEAFRSAIKDSGYDARIIGE
                     KEHNHQIVPEMFYEIERSKFMVVDVTHQNYGAYYEAGYAQALGKEVIVCCSKEVFDDP
                     KKKPHFDIAQKPIIIWTDLDDLKSRLIRRIQATVH"
     gene            complement(645..2135)
                     /locus_tag="PLR57_02960"
     CDS             complement(645..2135)
                     /locus_tag="PLR57_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQQ40444.1"
                     /translation="MKKFNRALALCLILLLLPFPAARAEGESAPVEISDAAGMLAIKD
                     NPSGSYVLTADIDMKGVDWLPVPFSGTFDGGGHVLYNLSITRMSEDTSITYDGRHRGY
                     HTVYASLFSVVENATIQNLNLLNLKINASTDQPCFAAGIAGHIERSSILNCSVKGRIV
                     INGTSRQCGVGGIAGFGNGLIQNCSVEAELTVIAVNPEVASEEYLGGILANGYADLDG
                     CSVVLAGYASVHGYAHNGGLIGIDDINPRNRRHSGYVKNNTVDASITFYEETDDRRAY
                     CEAYLGENQNDTVIVSANKTTRFKSNEVTDFSRPLRADMDENPVYEAVVTPPRDTEMG
                     YTTYTNKNNGYSYTDDYTAPGHTPGDWVTESEPTYESEGVKRQYCSECGALLGEETIP
                     KLVAVSSCQIDQTQLSLEYRSTYQLSAAVLPENALDTSVTWSSSNEAVATVDQTGLVT
                     AVEKGAVTITCRSNDGFASSDCQVEVYMTRGQWIVRYVLFGWLWDK"
     gene            complement(2295..>2948)
                     /locus_tag="PLR57_02965"
     CDS             complement(2295..>2948)
                     /locus_tag="PLR57_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40445.1"
                     /translation="YLGFCAFALLTDLAWLFLDVPYRLFAWFSLAQVPACLLLYYLFP
                     NEVTLTEYKKQANGRVMIAFVLLITAFVPLLRLMIDFNIRAWGALLLIAGVTAALLLV
                     QTLYLSPECRAQKRLLLTVGLAAAIWSVGAVGITNALFDSNPPAEKPAVVQEMQITHS
                     AKSPDRYLITAEAEDGQDFELSVAKKLYDSLAVGDTVTVSLYQGAYNIPYADAADAP"
BASE COUNT          671 a          817 c          796 g          664 t
ORIGIN      
        1 gtttaagaga gcccgcacct tataaaatct gtccgactta ctgttgacaa tccacgtatc
       61 atatagaatt cctctgttgc taaaaagagc actcttctgt tctataacta ctaatatagt
      121 tgagagtaat caagttctat cttatctttg cgatctcggt ttactgttat atgattcaga
      181 agcttataca tataacatca cctatgcggg gtgggtacaa atagatagta tgagaaagca
      241 gaaggactct gaatatcagg gctttattgc cataagctat tcggaagaag caaagcctat
      301 tctcgaagca tttcgttccg caatcaaaga cagtggttat gatgctagaa taataggcga
      361 aaaagagcac aaccatcaga ttgtgcccga aatgttttat gagattgagc gaagcaaatt
      421 tatggtcgtc gatgttactc atcagaacta tggagcctat tatgaagctg gttatgcgca
      481 agcactcggt aaagaggtaa ttgtctgttg cagtaaagag gtttttgatg atccaaaaaa
      541 gaaaccccat tttgatattg cccaaaaacc aatcatcatc tggactgatc ttgatgactt
      601 aaaatcaaga ctcatccgcc gcattcaagc cacggtacac taacctattt atcccacagc
      661 cacccgaaca gcacataccg cacaatccac tgccctctgg tcatgtacac ttccacctga
      721 cagtcggacg aggcaaagcc gtcgttcgag cggcaggtga tggttaccgc gcccttttcc
      781 acggcggtga ccagccccgt ttgatcgacg gtggcgaccg cttcgttgga ggacgaccac
      841 gttacactcg tatccagcgc gttttcgggc agcacggcgg cgctgagctg gtaggtgctc
      901 ctgtactcga gcgaaagctg cgtttggtcg atctggcagg agctaacggc aacgagcttg
      961 ggaatcgtct cctcgccgag cagcgcgccg cattcactgc agtactgccg cttgacgcct
     1021 tcgctttcgt aggtcggttc ggactccgta acccagtctc cgggcgtatg tccgggggcg
     1081 gtgtagtcgt ccgtatagct gtagccgttg ttcttattcg tgtaggtcgt gtagcccatt
     1141 tccgtgtcgc gcggcggggt gacgaccgct tcgtacacgg ggttttcgtc catatccgcc
     1201 cgcaggggac gcgaaaagtc ggtgacctcg ttactcttaa agcgcgttgt cttgttggcg
     1261 gaaacgatca ccgtgtcgtt ctggttttcg ccaagatacg cttcgcagta ggctctgcgg
     1321 tcgtcggttt cctcgtaaaa cgtaatgctc gcatcgacgg tattgttctt gacgtaccct
     1381 gaatgcctgc ggtttctggg gttgatgtcg tcaatgccga taagcccgcc gttatgcgcg
     1441 tagccgtgga cggaagcgta ccccgcgagc acgacgctgc agccgtcgag gtcggcgtat
     1501 ccgttggcga ggataccgcc aagatactcc tcgctcgcaa cttccggatt gacggcgatc
     1561 acggtcagtt ccgcctcgac cgagcagttc tgaatcaagc cattgccaaa ccccgcgatg
     1621 ccgcccacgc cgcactggcg gctggtgccg ttgatgacga tacggccttt gacggagcag
     1681 ttgagaatac tgctcctttc aatatgcccc gcgatgccgg cggcaaagca cggctggtcg
     1741 gtcgaggcgt ttattttgag gttgagaagg ttcaagtttt gaatcgtcgc gttttccacg
     1801 accgaaaaca gcgaagcgta caccgtgtga tagccccggt gccgcccgtc gtaggtgatg
     1861 ctggtatcct cgctcattcg cgtgatcgag aggttgtaga gaacgtgccc gcccccgtcg
     1921 aacgtgccgc tgaacggaac gggcagccag tccacgccct tcatgtcgat atccgccgtg
     1981 agcacatagc tgccggaagg gttatccttg atggcgagca tccctgccgc gtcggaaatt
     2041 tcgaccggcg cgctctcccc ttccgctctg gcggccggaa acggcagcag taataggatc
     2101 aaacaaagcg caagcgcgcg attgaacttt ttcacagaaa cgacctcctg cgcggttcat
     2161 tttcagccgg agtttagatg aatgaaccga taccccattg tagtccccgc gcgcttgttc
     2221 cgtcaacgcc acttaaatga aaacggcccc cgctccgtta agagcgaaag ccgctgatcg
     2281 ttcgattcgc aagattacgg cgcgtcggca gcgtccgcgt agggaatgtt gtacgcgccc
     2341 tgatagagag aaaccgtcac cgtatcgccg acggcgagac tgtcgtagag tttcttggcg
     2401 accgagagct caaagtcctg cccgtcttcc gcttccgccg tgatcaaata ccggtcgggc
     2461 gacttagcgc tgtgggtaat ctgcatttcc tgcaccacgg cgggtttttc cgcgggcggg
     2521 ttgctgtcga acagagcgtt cgtgattcca accgcgccga ccgaccagat ggctgcggcg
     2581 agcccaaccg tgagcagtaa acgtttctgc gcgcggcact ccggcgaaag gtagagcgtc
     2641 tgcaccaaaa gaagcgccgc cgttacgccc gcaatcaaaa ggagcgcgcc ccacgcgcgg
     2701 atgttgaagt ctatcatcag ccgcaaaagc ggaacaaacg cggtaatcag cagcacaaag
     2761 gcgatcatca cgcgcccgtt cgcctgcttt ttatactccg tcagcgtcac ctcgttggga
     2821 aagaggtaat acagcagcag gcacgcgggt acctgcgcca gcgaaaacca ggcaaagagc
     2881 cggtacggga cgtccagaaa cagccatgcc agatcagtca gcagcgcaaa cgcgcagaac
     2941 cccaagta
//
LOCUS       DAOMGY010000137         2943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_36245_length_2943_cov_3.967798, whole genome shotgun sequence.
ACCESSION   DAOMGY010000137 DAOMGY010000000
VERSION     DAOMGY010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36245_length_2943_cov_3.967798"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..2067
                     /gene="typA"
                     /locus_tag="PLR57_02970"
     CDS             253..2067
                     /gene="typA"
                     /locus_tag="PLR57_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HQQ40446.1"
                     /translation="MEIRNVAIIAHVDHGKTTLVDQMLRDAGVLRRGEQDVERIMDSN
                     ELERERGITILSKNTAVVYGGTRINIVDTPGHADFGGEVERILQMVDGVLLLVDAFEG
                     CMPQTRFVLRKALELKKVPIVVVNKVDRADSRPMEVVDEALELFIELGSSAEQLDFPV
                     VYASARDGMSGMTPNDMKPNMRALFDTILTAIPAPEFQPDEPLQILFSTIDYDEYVGR
                     IGVGRIERGTAKKGMPIVICGGESNRRQEAKITRLYRYEGLKRVEVDEAPCGDIVCVA
                     GVEGLNVGETVCDPACVEPLPFVKIDEPTVSMMFMLNDSPFAGREGKYVTGRNLRDRL
                     FTEVKTNISMRVEETGTTDSFRVSGRGELHLSILIETMRRQGYEFAVSRPRVILHTNE
                     AGEVTEPIEELTIDVPTEYVGAVMEKLGQRKAELVNMTNQGDNSVRLQFLVPARGLMG
                     YRSELLTDTRGNGVMSHIFHDYEPYKGPIEERKTGSLVAHETGDTSSYGLFNTQERGK
                     LFVGPGIPVYEGQIVGENARSEDIVVNVCKKKHVTNMRASGSDDALRLTPAVTLTLEQ
                     CLEFIAEDELVEVTPKSIRMRKKLLTKDQRVKASNKQQ"
     gene            2168..2611
                     /locus_tag="PLR57_02975"
     CDS             2168..2611
                     /locus_tag="PLR57_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009332422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ40447.1"
                     /translation="MVQLIEIDRHNYLSVIDLAVSDEQKSFVASNMYSLAQAYVQPEC
                     IPFALYAENKPVGFVMYAMDEDDHEYWIYRLMIDKKYQRKGYGREAMHLLIDRIRADQ
                     DHSVIYISFEPENAAAKALYESLGFVPDGRVEYGEVVYKLEKVEE"
     gene            2747..>2943
                     /locus_tag="PLR57_02980"
     CDS             2747..>2943
                     /locus_tag="PLR57_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40448.1"
                     /translation="MAFLFKISLARDAASYANVCLAAGKKRQVLLDSGAKKRYFSFRR
                     DNTMQLNDMLPFLIPLAVAEL"
BASE COUNT          716 a          796 c          803 g          628 t
ORIGIN      
        1 aatgttctat ttgcttaata cagttataca cgtctccgca gggcttgtca agcattattt
       61 tgagcagaaa ttcgaacccg ttctccgcag ctagctgttt aacaagataa cccgccgtat
      121 caagcgaaaa cagcgcaaaa aaaacggagc gaacgtaaaa agcgtgtgag tattttttcc
      181 ctatcttgtg cttcttggcg aacggttgta taataacttg tttttgtata ggagaaagaa
      241 ggaagtatta ccatggaaat tcggaacgtc gccatcatcg cgcatgtcga ccacggcaag
      301 accacgctgg tcgatcaaat gctgcgtgac gcgggcgtac tgcgcagagg cgaacaagac
      361 gtcgagcgca ttatggactc caacgagctg gagcgggagc gtggaatcac gattctctcc
      421 aagaacaccg ccgtggtcta cggcggcacg cgcatcaata ttgtagacac accgggccat
      481 gcggatttcg gcggcgaggt ggagcgcatc ctccagatgg tggacggcgt tctgttgctc
      541 gttgacgcgt tcgagggctg catgccgcag acgcgctttg tgctcagaaa agcgctcgaa
      601 ctcaaaaagg tccccatcgt cgttgtcaac aaggtagacc gtgcggattc ccgcccgatg
      661 gaagttgtgg acgaggcgct ggagctcttt attgaactcg gctcttctgc ggaacagctt
      721 gatttccccg tggtttacgc ttccgcgcgg gacggcatgt ccggcatgac ccccaacgac
      781 atgaagccga atatgcgcgc cctgttcgat acgattctca cagcgattcc cgcgccggag
      841 tttcagccgg acgaaccgct gcagattctg ttttccacca tcgactacga cgagtacgtc
      901 ggccgcatcg gcgtcggccg catcgaacgc ggaaccgcga aaaagggcat gcccatcgtg
      961 atctgcggcg gtgagagcaa ccgccgtcag gaagccaaga ttacgcgcct ctaccgctac
     1021 gaaggcctca agcgcgtgga agtggacgaa gccccctgcg gcgatatcgt ctgcgtcgcg
     1081 ggcgtggaag gcctaaacgt aggcgaaacg gtctgcgacc ccgcctgcgt cgagccgtta
     1141 ccgttcgtga agatcgacga gccgaccgtt agcatgatgt tcatgctcaa cgacagtccg
     1201 tttgccgggc gcgaaggaaa gtatgtcacc gggcggaacc tgcgcgaccg cctgtttacc
     1261 gaggtcaaaa ccaacatctc catgcgcgtg gaagaaaccg gcaccaccga ctccttccgc
     1321 gtaagcggcc gcggtgagct gcatctctcc atcctgatcg agaccatgcg ccgccagggg
     1381 tacgagtttg ctgtttcccg cccgcgcgtc attctgcata ccaacgaagc cggcgaagta
     1441 accgagccga tcgaggagct gaccatcgac gttccgaccg aatatgtcgg cgccgttatg
     1501 gaaaagctcg gtcagcgcaa agcggaactg gtcaacatga ccaatcaggg cgacaacagc
     1561 gtccgtctgc agtttctcgt tcctgcgcgc ggcctgatgg gttaccgttc ggagctcttg
     1621 actgatacgc gcggcaacgg cgtgatgagc catattttcc acgactacga gccttacaag
     1681 ggtcccattg aagagcgcaa gaccggttcg ctggttgcgc acgaaaccgg cgatacctca
     1741 agctacggcc tgttcaacac gcaggagcgc ggcaagctct ttgtaggccc cggcattccg
     1801 gtgtacgaag gccagatcgt cggcgaaaac gcgcgttccg aggacatcgt ggtcaacgtc
     1861 tgcaaaaaga agcacgttac caatatgcgc gcttccggtt cggacgatgc gctgcgcctc
     1921 acacccgccg ttacgctcac gctggagcag tgtctggagt tcatcgccga agacgagctc
     1981 gtcgaagtca caccaaagag catccgcatg cgcaaaaagc tgctcaccaa ggatcagcgc
     2041 gtcaaggcct ccaacaagca gcagtaaaca gcgcattcac ggaaaacagt tattttttcg
     2101 ttcaacagcc gcgccggtca acggcgcggc ttcccgctat cccgatatgt aaaaaggagt
     2161 ggaatgtatg gtgcagttga tcgagatcga ccggcataat tatctctccg taatcgatct
     2221 tgctgtttcg gacgagcaga agagctttgt agcgagcaat atgtactctc tggcgcaggc
     2281 gtacgtacag ccggagtgca tcccctttgc gctctacgcg gaaaataagc ccgtcggctt
     2341 tgtgatgtac gcaatggacg aagacgacca cgaatactgg atctaccggc tcatgatcga
     2401 caaaaaatat cagcgcaaag gctacggcag agaggccatg cacctgttga tcgaccgcat
     2461 tcgcgcggat caggatcaca gcgtcatcta tatcagtttc gagccggaaa acgccgccgc
     2521 caaggcgctc tacgaaagcc tcggcttcgt tcccgacggc agggtggagt acggtgaagt
     2581 tgtatacaag ctggaaaagg tggaggaata attcttttac tccacttttc tttataagaa
     2641 aagtggagta aagaatcctt ttattaagca caaaagtcat tatttcataa aagaaaagtg
     2701 gacaaaagaa cccttttatt aagtacaaaa gtcatttcga aaagaaatgg cttttttgtt
     2761 taaaatttct cttgctcgtg acgctgcgtc atatgctaat gtttgccttg cggcagggaa
     2821 gaagcgacag gttttgcttg attccggcgc gaaaaagcga tatttttcat tcaggaggga
     2881 taacactatg cagctcaacg acatgcttcc atttctgatt ccgcttgcgg ttgcggaact
     2941 cat
//
LOCUS       DAOMGY010000138         2926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_36549_length_2926_cov_2.744340, whole genome shotgun sequence.
ACCESSION   DAOMGY010000138 DAOMGY010000000
VERSION     DAOMGY010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36549_length_2926_cov_2.744340"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..957)
                     /locus_tag="PLR57_02985"
     CDS             complement(97..957)
                     /locus_tag="PLR57_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQQ40449.1"
                     /translation="MISETETQTTKEQPFDMHLHSSRSDGTDSPAEVIARAASLGITL
                     AALTDHDSVAGVSEALVAGKRVGVRVLPALEMDTEWPHELHILGLDVDIEEPRLYAAL
                     QTARERRVVRNDEIVRRLAEAGADVNPFLERDGSVATRLHFALALVAGGFATDIKDAF
                     RRYLRRGCAGYYTVERFPPKQVISLILGAGGVPVWAHPTHGSPNLHALTEELAGYGLM
                     GLEAFHPSVSEGESEVLVSIARQKGLLVTCGSDYHGANRAEVVPGQAWRKCAALEETR
                     AFFDARPVRE"
     gene            complement(1039..2925)
                     /gene="ftsH"
                     /locus_tag="PLR57_02990"
     CDS             complement(1039..2925)
                     /gene="ftsH"
                     /locus_tag="PLR57_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQQ40450.1"
                     /translation="MRNTEDDAAGKTIKAIQVNQREVYGLYGDSDSAAKDLPKKAEFY
                     VVIPSESTFRADMAAIVYAANPDQFSSVDQVSSADYSFAYESKIVEESIWSILLPYLL
                     IFGAFGVFYFFMMRAQSGGKQMTNFGKSRARLQTGDKLNVTFADVAGADEEKEELKEV
                     VDFMRNPARFNAIGARIPKGVLLVGPPGTGKTLLAKAVAGEAKVPFFSISGSDFVEMF
                     VGVGASRVRDLFQTAKRATPAVVFIDEIDAVGRHRGAGMGGGHDEREQTLNQLLVEMD
                     GFSPNEGIIVIAATNRPDILDPALLRPGRFDRQITVNYPDVKGREEILKVHARNKPLA
                     PEVELAVLAKRTPGFTGADLENVLNEAAILAARANKKTIGMTELEEAITRVQMGPEKR
                     SRVITESDKRITAYHEAGHAIVALKLEGCDPVHEVSIVPRGMAAGYTMYLPKEDMMHV
                     TKNKLLDRIAMALGGRVAEQLKFDDVSTGAYNDLQRSSEIAHKMVTEYGMSDSVGPIF
                     LGGQEEVFIAKDWGHQRNYSESLAAKVDDEVRKILDEQFERAKAAISSDMAALDRLAN
                     VLIEYERISGEEFAAILSGEDPSAVMGKPKKKAPAKRAPKAPKPEPDQGGMPNDAVPT
                     GSEA"
BASE COUNT          531 a          946 c          820 g          629 t
ORIGIN      
        1 cctcgaataa ttccggtgct cgagggcaga gccctcgaat tattcttccg gggctccgcc
       61 ccgaaccccg ctcaggggtc aaccccctga gaacccctac tctctcaccg gccttgcgtc
      121 aaagaacgcg cgcgtctctt ccagagcggc gcatttgcgc catgcctggc cgggaaccac
      181 ctcggcgcga tttgcgccgt ggtaatcgct gccgcaggtc acaagcaatc ccttctgccg
      241 cgcaatgctc acaagcacct cgctctcacc ttcgctcacg gagggatgga atgcctccag
      301 tcccatcagc ccgtaccccg ccagctcctc ggtcagcgca tgcaggttcg ggctgccgtg
      361 ggtaggatgc gcccaaacgg gtacgccgcc cgcgccaagg atgagcgaaa tcacctgctt
      421 gggcggaaaa cgttccaccg tatagtagcc cgcgcacccg cggcgtaagt aacgccggaa
      481 tgcgtccttg atatccgtcg caaacccgcc cgccacaagc gccagcgcaa agtgcaggcg
      541 cgttgcaacg ctgccgtcgc gctccagaaa cggattcacg tccgcgcccg cttccgcgag
      601 ccgccgcacg atctcgtcgt tacgcaccac gcgcctttcc cgtgccgttt gaagcgcagc
      661 ataaagtcga ggctcttcaa tatccacatc cagtcccaga atatgcagct cgtgcggcca
      721 ctccgtgtcc atctccagcg cgggcagaac gcgcacacca acgcgctttc cagccacgag
      781 cgcttccgac acaccggcaa cgctgtcatg atccgtcagc gcggcaaggg taatgccaag
      841 cgacgccgcg cgggcaatca cctctgcggg tgaatccgtc ccgtccgagc gactgctgtg
      901 caggtgcatg tcaaagggct gttcttttgt tgtttgtgtt tctgtttccg atatcataac
      961 ccgtattcct gtctacctga aaacgggacg gccaagccgc cccgtttctc tttgcaagtt
     1021 gtgcttcttg gttcttcgtt acgcttcgct gccggtcggt acagcatcgt tcggcatgcc
     1081 gccctgatcc ggttccggct tgggcgcctt gggcgcgcgc tttgcgggcg ctttcttctt
     1141 gggtttgccc atcacggcgg aagggtcttc cccgctcaga attgccgcaa actcttcgcc
     1201 ggagatgcgc tcgtattcga tgagcacatt cgccagacga tccagcgccg ccatatccga
     1261 gctgatggct gcctttgcgc gctcgaactg ttcgtccaga atcttgcgca cctcgtcgtc
     1321 aaccttggct gcgagagatt cggagtagtt tcgctgatgt ccccagtcct tggcgatgaa
     1381 gacttcttcc tgaccgccga ggaaaatcgg gccgacggag tcgctcatgc cgtactctgt
     1441 gaccatttta tgcgcaatct cgctcgaacg ctgtaaatcg ttgtacgcgc cggtggagac
     1501 atcgtcaaac ttgagctgtt cggcaacgcg cccgcccagc gccatggcga tgcggtcaag
     1561 cagtttgttc ttggttacgt gcatcatgtc ctctttgggc agatacatcg tgtaccctgc
     1621 cgccatgccg cgcgggacga tggagacttc gtgcacgggg tcgcatcctt ccagcttgag
     1681 ggcaacgatg gcgtggccag cctcgtggta tgccgtaatg cgcttatcgc tttccgtaat
     1741 cacgcggctg cgcttttccg gccccatctg cacgcgggtg atggcttctt caagctccgt
     1801 catgccgatg gtctttttat tggcgcgcgc ggcaaggatc gccgcttcgt tgagcacatt
     1861 ttccaaatcc gcgccggtaa atccgggcgt acgcttggcc agaacggcaa gctccacttc
     1921 cggcgcaagc ggtttgttgc gcgcgtgcac cttgaggatc tcctcgcggc ccttcacgtc
     1981 cggatagttc acggtaatct gccggtcgaa ccggccgggg cgcagcagcg ccgggtcgag
     2041 gatgtccggg cggttagtcg ccgcgatgac gataatgccc tcgttcggcg aaaagccgtc
     2101 catctccacg agcagctggt tgagcgtctg ctcgcgttcg tcgtgtccgc cgcccatgcc
     2161 agcgccgcgg tgacggccga ccgcgtcgat ttcgtcaatg aacacgaccg cgggcgttgc
     2221 gcgcttggcg gtctggaaca ggtcacgcac gcgcgatgcg cctacgccga cgaacatctc
     2281 cacaaaatcc gaaccggaaa tcgagaaaaa cggaaccttc gcttcacccg caacggcttt
     2341 tgccaagagg gttttacccg tgcccggagg gcctacgagc agaacgcctt tgggaatccg
     2401 cgcgccgatg gcgttgaagc gggcggggtt gcgcatgaaa tctacgactt ccttgagttc
     2461 ttccttctct tcgtccgcgc cggcaacgtc tgcaaaggtt acattcagct tatcgcccgt
     2521 ctgcaagcgt gcgcgggact tgccgaagtt cgtcatctgc ttgccgccgc tctgggcgcg
     2581 catcataaag aagtagaaca cgccgaacgc gccgaagatc agtaagtacg gcagaaggat
     2641 cgaccaaatc gactcttcta cgatttttga ttcataggca aacgaataat cagcgctgga
     2701 tacctgatcc acgctggaaa actgatccgg attcgccgcg taaacgattg ccgccatatc
     2761 cgcgcggaac gtggattcgg acggaatcac cacatagaac tccgctttct tgggcagatc
     2821 ctttgcggct gaatcgctgt cgccatagag gccgtagacc tcgcgctggt taacctgaat
     2881 cgctttgatg gttttgcccg ccgcatcgtc ttccgtgttc cgcacg
//
LOCUS       DAOMGY010000139         2924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_36583_length_2924_cov_3.294876, whole genome shotgun sequence.
ACCESSION   DAOMGY010000139 DAOMGY010000000
VERSION     DAOMGY010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36583_length_2924_cov_3.294876"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1875)
                     /locus_tag="PLR57_02995"
     CDS             complement(<1..1875)
                     /locus_tag="PLR57_02995"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQQ40451.1"
                     /translation="MHMSGEDKTRRIEISLPDTLPEMPNFEPNIRRAPDRGYRLSPMQ
                     TRQALKNALRYVPESLHETLAPEFLEELKTRGRIYAYRYRPEGRIYGKPIDQYKGNCL
                     AGKAFQVMIDNNLDFQVALYPYELVTYGETGQPCQNWLQYRLIKRYLEELTDEQTLVL
                     ESGHPLGLFPSRPEAPRVILTNALMVGMFDNLDDWEIAEQMGVANYGQMTAGGWMYIG
                     PQGIVHGTFNTILGVGRKYLGVPSDGDLTGKVFVSSGLGGMSGAQPKAANIARAVGVF
                     AEVDRSRIQTRLDQGWVETVSDDLDEVFSLALEAKRAGRSVSIAYHGNIVDLLEAAVA
                     REFPIDLLSDQTSCHNAYNGGYCPVGLTFAERTELLHRDRSAFHALVDASLKRHVAAI
                     RALTERGTRFFDYGNSFLKAVFDAGETWIAKNGLDTKDGFVFPSYVEDIMGPLLFDYG
                     FGPFRWVCLSGKHEDLVKTDRAAMECIDPSRCWQDRDNYVWIRDAEQNRLVVGTEARI
                     LYQDEEGRRRIALRFNELVRSGEIGPVMLGRDHHDVSGTDSPFRETANIRDGSNVMAD
                     MATQCFAGNAARGMTLCALHNGGGVGIGKAINGGFGLLLDGSARVDEIIESAISCDVI
                     G"
     gene            complement(1893..>2924)
                     /locus_tag="PLR57_03000"
     CDS             complement(1893..>2924)
                     /locus_tag="PLR57_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HQQ40452.1"
                     /translation="GETLSGAEAMRRAELEPVRLLAKEGLALINGTQIMTAVGALAVY
                     GAQKLVKTADIVAALTCEAQTCITGAFDERVHRLRAHPGQIACADNLRKLLSGSGLSK
                     ENVEGKVQDAYSIRCIPQIHGASRDAVEYAAEAVTREINAVTDNPLIFPDEDDVLSGG
                     NFHGQPMALAFDFLGIAIAEFADVSERRTERLVNPYLNNNLPAFLAPNGGLNSGFMIA
                     QYAAAALVSENKILAHPASVDSIPSSANQEDHVSMGTIAARKAAEILENAERVLAIEL
                     FAAGQALSMIGAERLAPATRAVYDALRKEVPFVEKDVVMYEQIGKCERLVASGAVLAA
                     AESVCGALN"
BASE COUNT          535 a          955 c          850 g          584 t
ORIGIN      
        1 gccgatgacg tcacacgaga tcgcactttc gatgatttcg tccacgcgcg cgctgccgtc
       61 cagcaaaagc ccgaacccgc cgttgatcgc cttgccaata cccacgccgc cgccgttatg
      121 cagcgcgcag agcgtcatgc cgcgcgcggc gttgcccgca aagcactgcg tggccatatc
      181 cgccatcacg ttgctgccgt cgcggatgtt cgcggtctcc cgaaacggcg agtccgtgcc
      241 ggatacgtcg tggtggtcgc gccccagcat cacggggccg atttcgccgg agcgcaccag
      301 ttcgttgaag cgcagagcaa tccgccgcct tccttcctcg tcctgataca ggatacgcgc
      361 ttcggtgccg acgacgagcc ggttttgctc cgcgtcccga atccaaacat agttgtcgcg
      421 gtcctgccag cagcggctgg ggtcgatgca ctccatcgcc gcgcggtcgg ttttgaccaa
      481 atcctcgtgt ttcccgctca ggcaaaccca gcggaacggg ccaaagccat agtcgaacag
      541 cagcggcccc atgatatcct ccacgtaaga ggggaacacg aatccgtcct tcgtgtccag
      601 accgtttttg gcaatccagg tttcgcccgc gtcgaacacc gctttgagaa acgagttgcc
      661 gtagtcgaaa aagcgcgttc cccgttcggt cagcgcgcga atggccgcaa cgtggcgctt
      721 gagcgacgca tccaccagcg catgaaacgc gcttcggtcg cggtgcagca actccgtgcg
      781 ctcggcaaac gtaagcccca cggggcagta tccgccgttg taggcattgt ggcaggaggt
      841 ctggtcgctg agaagatcga tcggaaactc ccgcgcaaca gccgcttcca gcaaatccac
      901 gatgttgccg tggtaggcaa tcgaaacgct cctgcccgca cgctttgctt ccagcgcaag
      961 cgagaatacc tcgtccagat cgtccgaaac cgtctccacc cagccctgat cgagccgggt
     1021 ctgaatgcgg gagcggtcaa cttccgcaaa cacccccacc gcgcgggcga tgttcgcggc
     1081 tttgggctgc gcgccgctca tgccgccgag gccggaactc acgaacacct tgcccgtcaa
     1141 atcgccgtcc gaaggcacgc caaggtactt tctgcccacg ccgagaatcg tgttgaacgt
     1201 gccgtggacg attccctgcg ggccgatgta catccagccg cccgcggtca tctgcccgta
     1261 gttggcaacg cccatctgct cggcaatctc ccagtcatcg aggttatcga acatgccgac
     1321 catcagcgca ttggtcaaaa tcacgcgcgg ggcttccggc ctgctcggga acagtcccag
     1381 cggatggccg gattccagca ccagcgtctg ctcgtcggtc agttcttcga gataacgctt
     1441 gataaggcgg tactgcagcc agttctggca gggctggccg gtttcgccgt aggtgaccag
     1501 ctcataggga tagagcgcaa cctgaaaatc caggttgttg tcgatcatca cttgaaacgc
     1561 cttgcccgcg aggcagttgc ccttgtactg gtcgatgggc ttgccgtaaa tacgcccctc
     1621 ggggcggtag cggtaggcgt aaatgcggcc gcgcgttttc agttcttcca agaactccgg
     1681 cgcaagcgtt tcatgcaggc tctccggcac gtaccgcagc gcgtttttga gcgcctgccg
     1741 cgtttgcata ggcgacagcc ggtagccgcg atccggcgcg cggcgtatat tcggctcgaa
     1801 atttggcatc tccggcagtg tgtccggcag cgaaatttcg attcttcgcg ttttatcttc
     1861 tccgctcata tgcattcgcc tccgtttttc aactaattca gcgcgccgca gacggattcc
     1921 gccgccgcaa gcacagcgcc gctcgccaca agccgctcgc atttgccgat ctgctcgtac
     1981 atcacaacgt ccttctccac aaacggcact tcttttcgca gcgcgtcgta aaccgcgcgg
     2041 gtagcgggcg cgagccgttc cgcgccgatc atggagagcg cctgcccggc cgcgaacagc
     2101 tcgatggcaa gcacgcgctc ggcattttcc aatatctccg ccgcttttcg cgcggcgatg
     2161 gtgcccatgc tcacatgatc ctcctgattg gcggacgacg gaatggagtc cacgctggcg
     2221 gggtgcgcga gaatcttgtt ttcggaaacc agcgccgccg ccgcgtactg ggcgatcata
     2281 aacccggagt tcaaccctcc gttcggcgcg agaaatgcag gcagattgtt gttgagatac
     2341 gggttgacca gccgttccgt gcgccgctcg gaaacgtccg cgaattccgc aatcgcaatg
     2401 cctaaaaagt cgaacgccag cgccataggc tggccgtgaa aattgccgcc ggagagcacg
     2461 tcgtcctcgt ccggaaagat cagcgggttg tcggtcacgg cgttgatttc ccgcgtgacg
     2521 gcttccgccg cgtactccac ggcgtcccgg cttgcgccgt gaatttgcgg aatacagcgg
     2581 atggaatacg cgtcctgcac ctttccttca acattctctt tggaaagccc gctgccggaa
     2641 aggagctttc tgaggttatc tgcgcaggca atctgcccgg gatgcgcgcg caggcggtgc
     2701 acccgctcgt cgaatgcgcc tgtaatgcag gtctgtgctt cgcaggtcag cgcagcgacg
     2761 atatccgccg ttttgacgag tttttgcgcg ccgtaaacgg ccagcgcgcc gacggcagtc
     2821 ataatctgtg tgccgttgat gagcgcaagc ccttcctttg ccaacagccg gacgggctcc
     2881 agctccgcgc ggcgcattgc ttccgcgccg gagagcgttt cgcc
//
LOCUS       DAOMGY010000140         2923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_36600_length_2923_cov_3.418759, whole genome shotgun sequence.
ACCESSION   DAOMGY010000140 DAOMGY010000000
VERSION     DAOMGY010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36600_length_2923_cov_3.418759"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLR57_03005"
     CDS             <1..283
                     /locus_tag="PLR57_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HQQ40453.1"
                     /translation="ITDETVLRAIRLHTTGEDGMSDFDALVYAADLIEPTRTFLSVEE
                     YRRRVRDGADAFMRYSLQRVSRLIAQRGWAVHPASARALEYYEKKAAAK"
     gene            362..748
                     /gene="rsfS"
                     /locus_tag="PLR57_03010"
     CDS             362..748
                     /gene="rsfS"
                     /locus_tag="PLR57_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQQ40454.1"
                     /translation="MGEQANQDAIRQRVEGICEILYNKKALDIVAIHVADKTIIADWF
                     VICSGRAVSQVKALADELDEKAPLLGLDLRRSEGYTEGKWVVLDYADILVHIFYPEER
                     KYYNMERLWDEGGGAIRYSEQKDVKA"
     gene            791..1003
                     /locus_tag="PLR57_03015"
     CDS             791..1003
                     /locus_tag="PLR57_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40455.1"
                     /translation="MEEVELRGIVKRFAQSGWDLIAQPAKEYLEGADCREELVEAVRQ
                     ADEECGSCGCEYDALYKRFLALIEEQ"
     gene            1324..>2923
                     /locus_tag="PLR57_03020"
     CDS             1324..>2923
                     /locus_tag="PLR57_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQQ40456.1"
                     /translation="MKPSRGRFVRLLAALAAVTLIFGASASLAAGETAVTITGVSVAG
                     KTYDGTPAEASGTPVAKDGDTTVTISSGDYVYTYSSTDGGGYSSATPPTNAGDYKLVV
                     SVSNGTYAGQSADIPFTIAKAAITVLPAVSISRTAGLSNPAFAYTSSGAVSGQTPAFT
                     GSLAGDAGTASMEGVYSITLGTLALADNGAFLANNYTLALVSGAAYRVYNYITDAVAT
                     VSTDNLGENGIYTGGVTLTAPDGYEIALAPGGAWGATATKTDCVNGSNAVYYYLRVSG
                     GASDGAISERKAVVVRYDATAPDVSLYSPLSGSTNAAIGANQKIILSATESVSAVSGK
                     TVTVNDGSMDHTAPASSATLVGNASSGLWYLVFDISDFSDSLSLANATQYTVSIETGA
                     YIDRAGNASGSSSAQFTTVAADTLTDPVAVVFTPANTQLSVRTNTDAAVASGQALERG
                     TILTFNAPSDAGFTQQQTTLVGGASAEVTLTNYALTGDLEVYYTRTPGAFDGTAAITG
                     TAKAGETLSGSVQSPTQTNDYYWTF"
BASE COUNT          664 a          815 c          871 g          573 t
ORIGIN      
        1 cattacggac gaaacggttc tccgcgccat ccgcctgcac accacgggcg aagacggtat
       61 gagcgatttc gacgcgttgg tctacgctgc ggatttaatt gaacctacgc ggacgttcct
      121 cagcgtggaa gaataccgca ggcgcgttcg cgacggcgcg gacgcgttta tgcgctactc
      181 gctgcagcgt gtttcgcgtt tgatcgcgca gagaggctgg gcggtgcatc ccgcgagcgc
      241 gcgcgcgctg gagtattatg agaaaaaagc ggccgcgaag tgaccgttaa gttccgcagc
      301 agcctttcgc ggctgcgaag aaagaaacag tataagtaca aagattatcg gagggaattc
      361 attgggcgaa caagcaaacc aagatgccat tcggcagcgg gtagagggaa tctgcgagat
      421 cctgtacaac aaaaaggcgc tggatatcgt tgccatccat gtggcggaca agaccatcat
      481 agccgactgg tttgtcattt gcagcgggcg cgcggtatcc caggtcaaag cgcttgccga
      541 cgaactggac gaaaaagcgc ccttgctggg gctggatttg cgcagaagcg aagggtacac
      601 ggaagggaaa tgggtcgttc tggattacgc ggatatcctt gtgcacattt tttaccccga
      661 agagcgcaag tattacaaca tggagcgcct ttgggacgaa ggcggcggcg cgattcgcta
      721 ttccgagcaa aaggacgtaa aagcgtaagc cgccgggaat caacgcaaac acgcaaagga
      781 ggagaattcg atggaagaag tggagctccg gggaattgtc aaacggtttg cgcagtccgg
      841 ctgggattta atcgcccaac cggcaaaaga gtatctcgaa ggggcggact gccgggaaga
      901 actggtagaa gccgtccggc aggcggacga agaatgcggc tcctgcggtt gcgaatacga
      961 cgcgctgtac aagcgctttc tggcgttgat cgaagagcag taaccgaaca gcgttaagca
     1021 aattaaaaag cacggagatg cgttatttta cgcgccgtgc ttttttttac ttctactttt
     1081 gaaaaatttg caaacgaact gcaacgattt cggcttttgc gcagtgttac ccatagagaa
     1141 ggggtttggc cgtttgcaaa ggaaaagtga ttgtggcata ctgagactgc taaaaggcga
     1201 acgagcgaaa aatcagccgg tcaagaagaa aaagaagttc gtccgccgga gccaacccat
     1261 tccagtaccg aaggccaatc ccttcgtcaa taatccattc gttgtccacg gaaaggagcg
     1321 catatgaaac cttcgagagg acgttttgtc agactgcttg ccgcgcttgc ggcggtgacg
     1381 ctgatctttg gcgcgtcggc ttcgcttgcg gcaggagaaa cggcggttac gattaccggc
     1441 gttagcgttg cagggaaaac ctacgacggc acacccgcgg aagcgagcgg aacgccggtt
     1501 gccaaagacg gcgacacgac ggtaaccatt tcctccggcg attatgtcta tacctactcc
     1561 agcacggacg gcggcggata ttcatccgca acgccgccga caaacgcggg ggactataaa
     1621 ctcgtcgttt ccgtttccaa cggcacttac gcgggacaaa gcgcggacat tccgtttaca
     1681 attgcaaaag ctgccatcac ggtgcttccg gcggtgagca tcagccgcac tgcggggctt
     1741 tccaacccgg cgtttgcgta tacttcctcc ggcgcggtct ccggccagac cccggcgttt
     1801 accgggagcc ttgcgggcga cgcgggtacc gcctcgatgg agggcgttta cagcatcacg
     1861 ctcggaacgc ttgcgctggc ggacaacggc gcgtttctgg caaacaacta cacgctcgcg
     1921 ctcgtttccg gcgcggcata ccgcgtatat aactacatta ccgacgccgt tgcaaccgtt
     1981 tcgaccgata accttggcga aaacggcatc tataccggag gcgtcaccct gaccgctccc
     2041 gacggatacg aaatcgcgct cgcgcccggc ggcgcctggg gcgctaccgc caccaagacg
     2101 gactgcgtta acggttcgaa cgccgtgtac tattacctgc gcgtgtccgg cggcgccagc
     2161 gacggcgcaa tctccgagcg caaggccgtt gtggttcgtt atgacgcgac ggctccggac
     2221 gtttcgctct actcgccttt gagcgggtca acaaatgcgg ccatcggcgc aaatcaaaag
     2281 atcattctct cggcaaccga atccgtttcg gcggtctctg gcaaaaccgt gaccgtcaac
     2341 gacggctcta tggaccatac cgctccggct tccagcgcta cgcttgtcgg caacgcgtcc
     2401 agcgggcttt ggtatctcgt gttcgacatc agcgactttt cggattctct ctcgcttgcc
     2461 aatgcaacgc agtacaccgt ttctattgag acgggcgctt atatcgaccg cgcggggaac
     2521 gcaagcggct cttcgagcgc tcagtttacc accgtcgcag cggacacgct gacggatccc
     2581 gtcgcggtgg tgtttacgcc cgcgaacacg cagctcagcg tgcgtacgaa tacggacgca
     2641 gccgttgcga gcgggcaggc gctggagcgc ggcacgattt tgacctttaa cgcgccttcg
     2701 gacgcgggat tcacccagca gcagacgact ctggtcggcg gcgcttccgc ggaagtcacc
     2761 ttgaccaact atgcgttgac gggcgattta gaagtgtatt acacccgtac gccgggcgct
     2821 ttcgacggta ctgcggcaat caccggaacg gcaaaagcgg gtgaaacgct gagcggatcg
     2881 gtgcagagcc cgacgcagac caacgattat tactggacgt tca
//
LOCUS       DAOMGY010000141         2921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_36637_length_2921_cov_3.087230, whole genome shotgun sequence.
ACCESSION   DAOMGY010000141 DAOMGY010000000
VERSION     DAOMGY010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36637_length_2921_cov_3.087230"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /gene="rmuC"
                     /locus_tag="PLR57_03025"
     CDS             complement(<1..904)
                     /gene="rmuC"
                     /locus_tag="PLR57_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HQQ40457.1"
                     /translation="MEITILVVSLCVFVLTLLALLFLYRSQQTFQKEFRSEQTRALSE
                     LRRELADGVQSSVSRLGSMILEQQRTSDAQGETRFKTFEVTNEQKLEAIRLTVERRLI
                     QLQTENAQKLDEMRKIVDEKLQKTLETKVAQSFQLVNERLEQVYKGLGEMQTLAAGVG
                     DLKKVLSNVKTRGMLGEIQLGAILEEILAPEQYERDVVIKPGSKENVEYAIKIPAADG
                     ISVLLPIDSKFPADLYAVLQDAYESGSKDAVNAARNALIQRIRMEATKIRDKYIDPPH
                     TTDYAFMFLPFEGLYAEVTSRGLVE"
     gene            complement(923..1573)
                     /locus_tag="PLR57_03030"
     CDS             complement(923..1573)
                     /locus_tag="PLR57_03030"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HQQ40458.1"
                     /translation="MPYLALALYAADVTIHLFACASKNRLMLRRVTKCLLMPLLALCY
                     VLFAKTFSQNVLAAILFGFAGDLVLLFRPRHWAFPAGITAFAAGHVFYIVSFLSALPQ
                     KPAWYWFVLLGIISLTSAAVLLKYLWKGLPKKLRPPSFLYMLLIGAMASCAVLFGFSG
                     VSPLCWLAAIGGLLFVISDTTLSIDAFHHTVKYRNVIVMSTYIAAQTLIVAALALT"
     gene            1793..2485
                     /locus_tag="PLR57_03035"
     CDS             1793..2485
                     /locus_tag="PLR57_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40459.1"
                     /translation="MQYSHEVEEMECVLKGAKHDPAPIPEEGKWVKAKQITDISGLTH
                     GVGWCAPQQGACKLTLNVKNGVIEEALVETLGCSGMTHSAAMAAEILPGKTILEALNS
                     DLVCDAINTAMRELFLQIVYGRTQSAFSDDGLPIGAGLEDLGKGLRSQIGTMYGTKCK
                     GARYLEMAEGYVLAIGLDEGGEIIGYKFVSLGKMMDAIKKGVDPKEAYEKNIGTYGRF
                     DQAAKVIDPRKA"
     gene            2496..>2921
                     /locus_tag="PLR57_03040"
     CDS             2496..>2921
                     /locus_tag="PLR57_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HQQ40460.1"
                     /translation="MATFEGYERRIDKINAELKKYGINGLDDAKAIVDAKGIDVFGIV
                     RGIQPIAFENACWAYTVGAAIAIKTGATTADKAAEAIGVGLQAFCIPGSVADQREVGL
                     GHGNLAAMLLREETSCFCFLAGHESFAAAEGAIGIAKTAN"
BASE COUNT          691 a          800 c          855 g          575 t
ORIGIN      
        1 cttccacaag cccgcggctc gtgacctccg catagagccc ttcgaacgga aggaacataa
       61 acgcgtagtc ggtcgtatgc ggcgggtcga tgtatttatc ccgtatcttt gtcgcttcca
      121 tgcggattcg ctgaatcaga gcattgcgcg cagcgttgac tgcatccttg gagccggatt
      181 cgtaggcgtc ctgcaatacg gcgtacagat cggcggggaa tttggagtcg atgggtagca
      241 gaacggatat gccgtcggca gcgggaatct tgatggcata ttcgacgttt tccttggagc
      301 cgggtttaat cacaacgtcg cgctcgtatt gttcgggtgc gaggatttct tccagaatcg
      361 ctccaagttg aatttctccc agcatgccgc gcgtcttcac gttggagagc acctttttca
      421 aatcgcccac gccggcggca agcgtctgca tctctccaag gcctttgtag acctgctcga
      481 gccgttcgtt taccagttgg aaggattgcg cgaccttggt ttccagcgtt ttctggagct
      541 tctcatccac gatcttgcgc atctcgtcga gcttctgtgc gttttccgtt tgcaattgga
      601 tcagtctgcg ttcgacggtg agccggatgg cttccagctt ctgctcgttg gtgacctcaa
      661 acgtcttaaa gcgcgtttca ccctgcgcgt cgctggtgcg ctgctgctcg aggatcatgc
      721 tgccgagtct ggaaacggag gactgtacgc cgtccgccag ctcgcggcgc agttctgata
      781 gcgcgcgcgt ctgttcgctg cggaattcct tttgaaacgt ctgctgtgag cgatagagaa
      841 acaacagcgc caacagcgtc agcacaaaga cgcagaggga aacgacaagg atggtgattt
      901 ccatgttcgg cgataacctc ctttaagtaa gcgccagcgc ggcaacaatc agcgtctgcg
      961 cggcaatata agtactcatt acgataacgt tgcggtattt caccgtgtgg tggaacgcgt
     1021 cgatggaaag cgttgtatcg gaaatgacga acagcagccc gccgattgcc gcaagccagc
     1081 agagcgggga aacgccggaa aaaccgaaca gcaccgcgca ggaagccatc gccccgatga
     1141 ggagcatata gagaaagctc ggcggacgca gctttttcgg cagtcccttc caaaggtact
     1201 tcagaaggac ggctgcgctg gtgaggctga tgattcccag cagcacgaac cagtaccagg
     1261 cgggtttctg tggcagggcg gagagaaagc tgacgatata aaacacgtgc cccgcggcaa
     1321 acgcggtgat gcccgccgga aatgcccagt ggcgcggacg gaacagcagt acgagatcgc
     1381 cagcgaatcc aaacaggatg gcggcgagaa cgttttgcga gaacgtcttt gcaaacagaa
     1441 catagcagag cgccaacagc ggcatgagca ggcacttcgt taccctgcgg agcatgagcc
     1501 tgtttttgct ggcgcaggca aagagatgaa tcgtaacgtc tgcggcgtag agcgcgagcg
     1561 caagatacgg caagcggttg ttcccccgat catttaactg caaacagtat accatagacg
     1621 cgccgtgcgc caaagtaaaa tcatccggtg ttgcttatcc tgcgtgattt gtatttttcg
     1681 aaaaacatgc cgaaatgtcc gaacattcgt gttgaaaacg ctatatcgat aatgctatac
     1741 tgtaacctga aacaatctgg caagagtgaa cactaaagga ggaattccac atatgcagta
     1801 ttctcatgaa gtggaagaaa tggaatgcgt cctcaagggc gccaagcatg atcccgctcc
     1861 gatcccggaa gagggcaagt gggtcaaagc aaaacagatc accgatatca gcggcttaac
     1921 gcacggcgta ggctggtgcg cgcctcagca gggcgcctgc aagctcacct tgaacgtcaa
     1981 gaacggcgta atcgaagaag cgctggttga aaccctcggc tgcagcggca tgacgcactc
     2041 cgcggccatg gcggcggaaa ttctgcccgg caagaccatc ctcgaagcgc tcaacagcga
     2101 cctcgtctgc gacgcgatca acaccgccat gcgcgagctg ttcctgcaga tcgtctacgg
     2161 tcgcacacag agcgcgtttt cggacgacgg cctgcccatc ggcgcgggtc tggaagatct
     2221 cggcaaaggg ctccgctcgc agatcggcac catgtacggc acaaagtgca agggcgcgcg
     2281 ctatctggaa atggcggaag gctacgtcct cgccatcggc cttgacgaag gcggcgagat
     2341 catcggctac aagttcgtca gcctcggcaa gatgatggac gccatcaaga agggcgtcga
     2401 tcccaaggaa gcgtacgaaa agaacatcgg tacctacggg cgttttgacc aggccgccaa
     2461 ggtaatcgac ccgcggaaag cgtaaggagg cagctatggc tacttttgaa ggatacgaaa
     2521 gaagaattga caagatcaac gccgaactca aaaagtacgg catcaacggc cttgacgacg
     2581 ccaaagcgat tgtagacgca aagggcatcg acgtgttcgg catcgtccgc ggcattcagc
     2641 ccatcgcgtt tgaaaacgcc tgctgggcat acacagtagg cgcggctatc gccatcaaaa
     2701 cgggcgcgac aaccgcggac aaagcggcgg aagccatcgg tgtgggtctg caggcgttct
     2761 gcattccggg ttcggttgcg gatcagcgcg aagtcggact cggccacggc aacctcgccg
     2821 ccatgctgct gcgcgaagaa acctcctgct tctgcttcct ggcagggcac gagagctttg
     2881 ccgccgcgga aggcgcgatc ggcatcgcaa agaccgccaa t
//
LOCUS       DAOMGY010000142         2908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_36886_length_2908_cov_3.225727, whole genome shotgun sequence.
ACCESSION   DAOMGY010000142 DAOMGY010000000
VERSION     DAOMGY010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36886_length_2908_cov_3.225727"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLR57_03045"
     CDS             complement(<1..309)
                     /locus_tag="PLR57_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HQQ40461.1"
                     /translation="MAKHILGAALAKARSEELKSSVEALKLNGVEPKLVIVRFGDQAG
                     DLAYERGIIRTFAPIGILVETRRLDEHAPQSELLDLFKALAQDPSVHGILPMRPFPRQ
                     I"
     gene            complement(332..964)
                     /locus_tag="PLR57_03050"
     CDS             complement(332..964)
                     /locus_tag="PLR57_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HQQ40462.1"
                     /translation="MEFHEQTIDSFLELLSSKAAVPGGGGASALVGSVAASLSHMVAA
                     LTVGKAKYADVEGEMQGVLAESERITKRFLSLMDEDASAFAPLAEAYRLPKDTPEQIT
                     ARTKIMEAALRTAVEPPLRIMEVCGEALELIAVCAEKGSVMAVSDAGVAATLCRAALE
                     GASLNVFINTQPMTDREYAERLNFRAKHMLAEYGTRAEELYKKVYAKLGA"
     gene            complement(975..2642)
                     /locus_tag="PLR57_03055"
     CDS             complement(975..2642)
                     /locus_tag="PLR57_03055"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQQ40463.1"
                     /translation="MMSDIEIAQANTPLPIGEVASALGIPVDALEPYGNYKAKIDVHK
                     LNLSPQKSGKLVLVTAMTPTPAGEGKTTTTVGLADALRRIGKRSVVALREPSLGPVFG
                     VKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNALNIDPRRI
                     VWRRCVDMNDRQLRFVVDGLGGRVNGVPREDGYDITVASEIMAVLCLANGILDLKERL
                     SRVVVAYTYDEKPVTAGDLNAVGAMAALLKDALKPNLVQTLEHTPALVHGGPFANIAH
                     GCNSVTATKLALQLGEYCVTEAGFGADLGAEKFLDIKCRVSELKPDAVVVVATVRALK
                     MHGGVPKPDLGKENLEALERGLPNLLRHVENIVSVYHLPCVVAINRFPGDTEAELKLV
                     ESRCGALGVNVALSEVWAKGGLGGVALANEVVRLCEQSNQFAFSYELDQSIEQKVEAI
                     VKRVYHGSGVLFAPAVKKQADQLTALGYGGLPICMAKTQYSFSDDPGLLGAPEGFTVT
                     VRNLRVSAGAGFLVALTGDIMTMPGLPKSPAAERIDVDEHGRITGLF"
     gene            complement(2695..>2908)
                     /locus_tag="PLR57_03060"
     CDS             complement(2695..>2908)
                     /locus_tag="PLR57_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HQQ40464.1"
                     /translation="FVTVTPNNPRAMQAEELAKLLQEHGLNAVSCPSVQEGVSKALEQ
                     VGNDGIVCALGSLYLLGDVRSILGVK"
BASE COUNT          582 a          865 c          831 g          630 t
ORIGIN      
        1 gatttgcctt gggaaaggcc gcatcggcag gatgccgtga acgctcggat cctgggcaag
       61 agccttgaac aaatccagca gttcgctctg cggcgcatgt tcgtcgagcc tgcgggtttc
      121 aaccagaatg ccgatgggcg caaaggtgcg gatgatgccg cgctcatagg caaggtcgcc
      181 cgcctgatcg ccaaaacgga cgatcacgag tttgggttct acgccgttta gcttcaaagc
      241 ttccacgctg gatttcaact cttccgaccg cgctttggct agcgccgcgc cgagaatatg
      301 ttttgccata tcgttcccct taacggcgcg gttacgcgcc gagctttgcg tatacttttt
      361 tgtagagttc ttccgcgcgc gtgccgtatt cggcgagcat atgttttgcg cggaagttca
      421 gccgttcagc atattcgcgg tcggtcatcg gctgcgtatt gataaaaacg ttcagactcg
      481 cgccttcgag cgcagccctg cagagggtgg cggcaacacc cgcgtcgctg acggccatga
      541 cggagccttt ttccgcgcag acggcgatca gttccagcgc ttcgccgcag acttccatga
      601 ttcttaacgg cggttcgacg gcggttcgca aggcggcttc cataatcttt gtccgcgcag
      661 taatctgctc gggtgtgtcc ttcggcaggc ggtaggcttc cgcaagcggc gcaaaggcgg
      721 aagcgtcctc atccatgagc gaaaggaaac gtttggtaat gcgctcggat tccgccagaa
      781 caccctgcat ttcgccttcc acatcggcgt atttcgcctt gccgacggtc agcgccgcaa
      841 ccatatgcga aagcgatgcg gcaaccgagc cgaccagcgc gcttgcgccg ccgccgccgg
      901 gaaccgcagc cttgctggag agcagttcga gaaaggaatc gatggtttgc tcgtgaaatt
      961 ccataattta ctccttaaaa cagtccggtg atgcggccgt gttcatctac gtcaatgcgt
     1021 tcggcggcgg gggacttggg cagtcccggc atggtcatga tgtcgccggt taacgccacg
     1081 aggaagccag ccccggcgga gacgcggagg tttcgcacgg tgacggtgaa cccttccggc
     1141 gcgccgagca aacccggatc gtcggaaaaa ctgtattgcg ttttcgccat gcagatgggg
     1201 agtccgccat atccgagcgc agtgagctga tcggcttgct tcttcaccgc gggagcaaag
     1261 agaacgccgc tcccatggta gactcgcttg acgatggctt ccactttttg ctcgatcgac
     1321 tgatccagct catagctgaa cgcaaattgg ttcgactgct cgcagagacg caccacttcg
     1381 tttgccagcg cgacgccgcc taatccgcct tttgcccaaa cttccgaaag ggcgacgttt
     1441 acgccgagcg cgccgcagcg cgattcgacc aacttcagct ccgcttccgt gtctccgggg
     1501 aaccggttga tggcgacgac gcaggggaga tggtaaacac tcacaatatt ctcaacatgt
     1561 ctgagaagat tcggaagccc gcgttccagc gcttccagat tctctttgcc gagatcgggt
     1621 ttcggaacgc cgccgtgcat cttcaacgca cggacagtag caacaacaac gactgcgtcc
     1681 ggctttaatt cgctgacgcg gcacttgata tccagaaact tttctgcgcc gagatccgcg
     1741 ccgaatcctg cttccgtgac gcagtattcg ccaagctgaa gtgcaagctt cgtcgcggta
     1801 acggaattac agccgtgcgc aatgttggca aacggccctc cgtgcacgag cgcgggcgta
     1861 tgctccagcg tctgcacaag attcggtttg agcgcgtcct tcagaagcgc cgccattgcg
     1921 ccgaccgcat tcagatcgcc tgcggtcacg ggcttttcgt catacgtata agcaacgact
     1981 acgcgggata agcgttcttt taaatcgaga atgccgttgg cgaggcagag aaccgccatg
     2041 atctcgctgg cgacggtgat gtcatacccg tcctcgcggg gaacgccgtt gaccctgccg
     2101 ccaagaccat ctacgacaaa gcgaagctga cggtcgttca tgtccacgca cctgcgccag
     2161 acgatcctgc gcgggtcgat attcagcgcg ttgccctgat aaatatggtt atccagcatc
     2221 gcggccagca agttgttggc ggctccgatg gcatgaaaat ctcccgtaaa gtggaggttg
     2281 atatcctcca taggcacaac ctgcgcatag ccgccgcccg ccgcgccgcc ttttacgcca
     2341 aagacaggcc cgagcgaagg ttcccgcaag gctacgaccg aacgtttacc gatcctgcgc
     2401 agcgcgtcgg caaggccgac cgttgtagtc gttttgcctt ctccggcagg ggtgggggtc
     2461 atggctgtga caagaacgag ctttccggac ttctgtgggg agaggttcag cttatgcaca
     2521 tcgattttag ctttgtagtt gccgtaaggc tcgagcgcgt ccaccggaat gccgagagca
     2581 gaagcgactt cgccaatggg cagcggtgtg tttgcctgcg cgatttcgat atcgctcatc
     2641 attgccatga gagttcctcc ctgattgccg caaattttcg caatactgaa tctgttattt
     2701 cacgccgagg atgctccgca catcccccag taaatacagc gaacccagcg cgcagacgat
     2761 accgtcgtta cccacctgtt ccagcgcttt tgaaacgcct tcctgcacgc tcgggcagga
     2821 aacggcgttc agcccgtgtt cctgtaaaag cttcgcaagc tcttccgcct gcatggcgcg
     2881 gggattgttg ggcgtcaccg tgacgaag
//
LOCUS       DAOMGY010000143         2887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_37285_length_2887_cov_2.852754, whole genome shotgun sequence.
ACCESSION   DAOMGY010000143 DAOMGY010000000
VERSION     DAOMGY010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37285_length_2887_cov_2.852754"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLR57_03065"
     CDS             <1..217
                     /locus_tag="PLR57_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40465.1"
                     /translation="VAATVYASQPAGSETLVTLTVGKVEFLAIQIGLTEYEINQKVFA
                     VLHPSRMNVYSKATGRLIKFASAKRIK"
     gene            295..1593
                     /locus_tag="PLR57_03070"
     CDS             295..1593
                     /locus_tag="PLR57_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40466.1"
                     /translation="MKAKRLLAWLLTLALLLSVTSVFGCAKNDGAAEEPAAQGTEAEE
                     PAGPDYENMSMDELYELAKLETGTITIYSTTTTAQTAVKKFVKAYPEFDGRIEYIESD
                     TNTVADRIETESDTGNINADVLQVKDNSGEIFYELVQYDYLDVFYPASVCSHVDPELL
                     KYGMPVYATYSPWYYNTETFPDGCPLTSWWDIVQGYDTTTRSFIDASGTNNQYWTIYT
                     KDITSASYAALWAQLIVDGDAIAQQYETQYGKPLEYTYMDSLTNSEGVMEFPENNGGV
                     ELFWRFTQMAITELDDGDQVVSAVDESLNGPTLGLCSGSKLDNVNNGLTINWVTGLEP
                     YTAFKACDYVYPIKGSDNPAGARLFILFMLGGDDGQSGCLNAFNAIGKWSIRDDFVFD
                     KTPYTAEEVNLKNPDFEDIYSFYPDVKAYWIYWRSLAPST"
     gene            1671..2183
                     /locus_tag="PLR57_03075"
     CDS             1671..2183
                     /locus_tag="PLR57_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40467.1"
                     /translation="MANSKESPAGGTLRDKLDRFWRILFLNEQGRPKSAMFLYSFCLS
                     LLFFAIYAASYFFLIDVLESAFATSSVLMRNILQSVLPGLLGTIACCALFYVFPDKRL
                     VPVAYLWLAVFALAALITMACITEREEFRIFLYFFAMLIPAGLLSGGLISFLQFRRYR
                     QKAEKAKEAC"
     gene            2177..>2887
                     /locus_tag="PLR57_03080"
     CDS             2177..>2887
                     /locus_tag="PLR57_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ40468.1"
                     /translation="MLKLDFLNVGDGDAILVRDDAADFVMLVDTGRPHIEFTKGSRRT
                     SVINHLMRERIDHIDLLVLTHLHFDHIGGTLPVLRHIPVRRLLAVTLPPEGAPWIDSS
                     VSEEKTIVGLCDALNLYSDIVSAARESGTLCETASPTTERLTPQLTMLPVLPEEALIH
                     RQEKQFDAIFRGEPLSEDVLYAVSKERNISSMRLRLTYAGRTVLLTGDSYAAYWENDP
                     EEPCDILKVPHHGDEKSMT"
BASE COUNT          639 a          883 c          761 g          604 t
ORIGIN      
        1 cgttgcggca accgtgtatg ccagccagcc cgccggcagt gaaacactgg ttaccctcac
       61 ggtcggcaag gttgaattcc tcgccatcca gatcggcctg accgaatacg aaatcaacca
      121 gaaggtgttt gccgttctgc atccttcgcg catgaacgta tacagcaaag cgaccgggcg
      181 gctgatcaag ttcgccagcg ccaaacgcat taagtaacgc aatatctatc cggggaagcg
      241 gctgacgccg tttcccctta cgactaataa ttcacgcaag gaggagagaa tttcatgaaa
      301 gcaaaacgac tgcttgcctg gctgctgacg ctggcgctgc tcctgtcggt cacctcggtg
      361 ttcggctgcg ccaaaaacga cggcgctgcg gaagaaccgg ccgcacaagg taccgaggcg
      421 gaggaacccg cagggccgga ctacgagaac atgagtatgg acgagctcta tgagctcgcc
      481 aaactggaaa caggcacgat tactatttac tccacgacga cgaccgccca gaccgccgtc
      541 aaaaaattcg tcaaggctta ccccgaattc gacggccgca tcgaatacat tgagtccgat
      601 acgaatacgg ttgccgaccg cattgaaacc gaaagcgaca cgggcaacat caacgcggac
      661 gtgctgcagg ttaaggacaa cagcggagaa atcttctacg agctggtgca gtacgactac
      721 ctcgacgtct tctaccctgc cagcgtctgc tcgcatgtcg accccgaact gcttaagtac
      781 ggcatgccgg tctacgccac ctacagtccc tggtactaca atacggaaac atttccggac
      841 ggctgccctc tgaccagctg gtgggatatc gtgcaggggt atgacacgac cacccgttcc
      901 ttcattgacg cttccggcac gaacaaccag tactggacga tctacaccaa ggacatcacg
      961 agcgcttcct acgccgccct ctgggcacag ctcatcgtgg acggcgacgc gattgcccag
     1021 cagtacgaaa cgcagtacgg caagcctctg gaatacacct atatggattc gctcacgaac
     1081 agcgaaggcg ttatggaatt cccggagaac aatggcggtg ttgaactctt ctggcggttt
     1141 acccagatgg ctattaccga gctggatgac ggcgatcagg tggtttccgc cgtggacgag
     1201 tccctgaacg ggccgaccct cggcctctgc tccggcagca agctggataa cgtcaacaac
     1261 ggccttacga tcaactgggt aacgggctta gaaccgtata ccgcatttaa agcctgcgac
     1321 tacgtgtatc cgattaaggg ttccgacaac cccgcgggcg cgcgtctgtt tattctcttc
     1381 atgctcggcg gcgacgacgg ccagagcggc tgtctcaacg cgttcaacgc gattggcaaa
     1441 tggtccatcc gcgacgactt cgtgtttgac aaaacgccgt acaccgccga agaagtcaac
     1501 ctgaagaacc cggactttga ggatatctac agcttctatc cggacgttaa ggcttactgg
     1561 atctactggc ggagccttgc gccgagcacc tgatacctat cttaacttga tcgcattggc
     1621 ggcagtccgt gtgcagccgc gcacggattg ccgcaatgga ggcttttttc atggcaaact
     1681 ccaaagaatc ccccgccggg ggaacccttc gggacaaact cgaccgattc tggcgcattc
     1741 ttttcctcaa tgaacagggc cgccccaaaa gcgccatgtt cctctattcc ttttgtctga
     1801 gcctgttgtt ttttgccatc tacgccgcat catacttctt tctcatcgac gtgctggaaa
     1861 gcgcgttcgc cacttcgagc gtgctaatgc gaaacatcct ccagagcgtg cttcccggcc
     1921 tgctgggaac catcgcctgc tgcgcgctgt tttacgtgtt ccccgataag cggctggtgc
     1981 ccgttgccta cctctggctt gcggtattcg cgcttgcggc gctgattacc atggcgtgca
     2041 tcacagagcg cgaagaattc cgcatctttc tgtacttttt tgcaatgctg attcccgccg
     2101 ggcttctcag cggcggcttg atttcgtttc tgcagttccg ccggtaccgc caaaaagccg
     2161 aaaaggcgaa agaagcatgc tgaaactcga ttttctcaac gttggcgacg gcgacgccat
     2221 tttggttcgc gacgacgcgg cggatttcgt catgctcgtg gatacgggca gaccgcacat
     2281 cgaatttacg aagggttcca gacgaacctc ggtaatcaac catctcatgc gcgaacgcat
     2341 cgatcatatc gacctgcttg tgctcacgca tctgcacttt gaccatatcg gcggcacgct
     2401 gcccgtactg cgacatatcc cggtacggcg gctcctcgcc gttactcttc cgccggaagg
     2461 tgcgccctgg atcgactcct ccgtttccga ggaaaagacc atcgtcggcc tttgcgacgc
     2521 gctgaatctc tacagtgata tcgtctccgc cgcccgcgag agcggcacgc tctgcgaaac
     2581 cgcaagcccg accaccgagc ggttgacccc gcagctcacg atgctccccg tcctgccgga
     2641 ggaagcgctg attcaccgcc aagagaaaca gttcgacgca atctttcgcg gcgagccgct
     2701 ttcggaagac gtgctctatg cggtttccaa ggagcgcaac atctccagta tgcgtctccg
     2761 gctgacctac gcgggaagaa ccgtcctttt gacgggagat tcctacgccg cctattggga
     2821 aaacgatccc gaagagccct gcgatatcct caaggttccg catcacggcg acgagaaatc
     2881 catgacg
//
LOCUS       DAOMGY010000144         2868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_37625_length_2868_cov_2.974760, whole genome shotgun sequence.
ACCESSION   DAOMGY010000144 DAOMGY010000000
VERSION     DAOMGY010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37625_length_2868_cov_2.974760"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1954)
                     /locus_tag="PLR57_03085"
     CDS             complement(68..1954)
                     /locus_tag="PLR57_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40469.1"
                     /translation="MSPTNQGYDEYDRARKRGLREFSLAKARGESGYLPVLDSKREEN
                     GVLGFIAQPMRPISLNRIAGTYQASRANSFAPNFMPLLSPDTEFANKWINLCNAHLES
                     GIRDPIRVYEYLWKYYVAEGNKRVSVLKYFEASSFEAEITRLVPQYDENDPAIERYYT
                     FLAYTKKGVFRDIELSESRKYERLYRIEQKLIAELDPAADAPDYNAILIRFETVYLNS
                     NCTLSLGDAFLEYLHVYGFPTNILPDELLSRIKTLKPQLELLEHPPEPRVVFESEAQA
                     AEPPLFTRLLQTHKNPRIVFAYSGERTQNNWLGAHEQGRLAMQAELGERVNSSVVDLP
                     DREDAYEILCREAADAGLLFVTTPSLMNPVLRFALEHPNCLTLVYSRMQHHYRLHTYF
                     GRYYEAVFLCGAAAGQFTKTGVTAYVTPKLNYARFTSDINAFAIGVRSVRPDAKVLLV
                     TRDVSPTEPDTCEIGVKTAASLGADVVLTPHYDSLSLADLPYSAFSALVSIDSTGKPL
                     RYLAAPDWNWERFYTAIVKSYLNGGLGALLNTDREESPITSFWWGLGGGVVDVRLGAW
                     SSGTPNNLLRYLRGSIARNLYNPFHGPVVDNEGVLRIPAHSDPPPAEIIKMRYLVEGI
                     ESIE"
     gene            complement(1951..2655)
                     /locus_tag="PLR57_03090"
     CDS             complement(1951..2655)
                     /locus_tag="PLR57_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQQ40470.1"
                     /translation="MKLRALVVSDHESEYIWDYFDASVFKGVDVIVSCGDLKAEYLSF
                     LVTMIPAPLLYVRGNHDGRYEQNPPEGCIDLEEKPVVIKGVRFVGFGGCKCAKTEANH
                     YSERDMARRVKRTESLLTLQRKGFDVLVTHAPAAGLGDGDDSFHEGFTAFRTLIDRFQ
                     PRYHLHGHQHLSYSIGSKRVIEYNQTTIVNGFNYYLMDMEFVPQTAKKKPAEPLDVLK
                     RKPPAPAKHELIKEKT"
BASE COUNT          592 a          829 c          842 g          605 t
ORIGIN      
        1 gcgggtgctc gagggcagag ccctcgaatc attccggggc tcgagggcag agccctcgaa
       61 acagccccta ctcgatactc tcaatacctt cgacgagata gcgcatcttg atgatctcgg
      121 cgggcggcgg gtcactgtgc gcgggtatgc ggagcacgcc ctcgttatcg accacaggcc
      181 cgtggaacgg gttgtagagg ttccgcgcaa tgctgccgcg caggtacctt aacaggttgt
      241 tcggcgttcc gctcgaccaa gcgcccaaac gtacatccac cacaccgccg ccaagccccc
      301 accaaaagct cgtgatgggc gattcttcgc gatcggtatt caacagcgca ccaagcccgc
      361 cgttgagata gctcttcacg atagcggtgt agaaccgctc ccagttccag tccggcgcgg
      421 cgagataccg cagcggtttg cctgttgaat cgatggaaac gagcgcggaa aacgcgctgt
      481 acggcaggtc cgcaagcgag agcgaatcgt aatgcggtgt cagtaccacg tccgcgccca
      541 gcgaagcggc ggtttttacg ccaatctcgc aggtgtcggg ttcggtcggg gacacatccc
      601 gcgtgaccag cagaaccttt gcgtccgggc gcacggagcg cacgccgatg gcaaaggcgt
      661 tgatatccga agtaaagcgc gcgtagttga gtttgggggt aacatacgcg gtcacgccgg
      721 tcttggtgaa ctgtcccgcc gccgcgccgc agagaaacac cgcttcgtaa taccgcccaa
      781 agtaggtatg cagccggtaa tggtgctgca tgcgggagta aacgagcgta aggcagttcg
      841 ggtgttcaag cgcaaagcgc agcacggggt tcatcagcga cggcgtggtc acaaacagca
      901 gccccgcgtc cgccgcctcg cggcagagaa tttcgtacgc gtcctcgcgg tcgggcaggt
      961 ccacaacgga ggagttgacc cgttcgccca gttcggcctg catggcaagc cgtccctgct
     1021 catgcgcgcc gagccagtta ttctgcgtgc gctcgccgga gtaggcaaac acgatgcgcg
     1081 ggttcttatg cgtctgcagc agccgcgtaa atagcggtgg ttcggcggcc tgcgcttcgg
     1141 attcaaacac cacgcgcggc tcgggcggat gctcaagcag ttcgagctgg ggtttgagcg
     1201 ttttgatgcg agagagcagc tcgtccggca gaatgttggt cggaaagccg tacacatgca
     1261 gatattccaa aaacgcgtcg ccgagcgaca gtgtgcagtt gctgttgagg tagaccgttt
     1321 caaagcgaat cagtatcgcg ttgtaatccg gcgcgtcggc ggcggggtcg agctcggcaa
     1381 tcagcttttg ctcgatacgg tagagccgct cgtacttgcg tgattcggaa agctcgatat
     1441 cccgaaacac gccctttttc gtgtacgcaa ggaacgtata gtagcgctcg atggcggggt
     1501 cgttttcgtc gtactgcggc acgaggcgcg taatctccgc ctcaaacgag ctcgcttcga
     1561 agtattttag tacgcttacg cgcttgttgc cctcggcaac atagtacttc cagaggtatt
     1621 cgtatacgcg gatcggatcg cggatgccgc tctccagatg cgcgttgcag aggttgatcc
     1681 acttgttggc aaactccgta tccggagaca gcagcggcat aaagttcggc gcaaaggagt
     1741 tggcacggct cgcctgatag gtgcccgcga tgcggttgag cgaaatggga cgcatgggct
     1801 gggcgataaa gccgagcacg ccgttttcct cccgtttgga gtcgagaacc ggcagatacc
     1861 cgctttctcc gcgcgctttg gcaagcgaga actcccgtaa tccgcgtttt ctcgcgcggt
     1921 catattcatc atatccctga ttcgtagggc tcatgttttc tccttaatca attcgtgttt
     1981 ggcaggcgcc ggcggtttgc gcttgagaac gtcaagcggt tcggcaggtt tcttcttcgc
     2041 cgtctgcggg acaaactcca tatccattaa ataatagttg aatccgttga caatcgtagt
     2101 ctggttgtat tcgatcaccc gcttggagcc gatgctgtag ctcaaatgct ggtgcccgtg
     2161 caaatggtaa cgcggctgaa agcggtcgat cagcgtgcgg aaggcggtaa acccctcgtg
     2221 gaagctgtcg tccccgtctc ccagcccggc tgcgggcgca tgcgtcacca gcacgtcgaa
     2281 gcctttccgc tggagcgtca gcagactctc tgttcgttta acgcgccgcg ccatatcgcg
     2341 ctcgctgtag tggttcgcct ccgtttttgc gcacttgcat ccgccaaagc caacgaaccg
     2401 cacgcccttg ataacaaccg gtttttcttc caggtcgatg cagccttcgg gcgggttctg
     2461 ctcgtatctt ccgtcgtggt tgccccgcac atagagaagc ggcgcgggaa tcatggtcac
     2521 aagaaaggaa aggtactccg ccttcaggtc gccgcaggaa acaatcacat ccacgccctt
     2581 gaacacgctt gcgtcgaaat agtcccaaat gtattcgctt tcgtgatccg aaacgaccag
     2641 tgcccggagt ttcatgtttg ttccgccttg atccgatatt cgcgcacgcc gctaaccttt
     2701 ggccgcgcac ttttcagtaa taatacgata cacgcttaca acgcgaaagt aaagcggacg
     2761 aaaaacccgc cgggtaaatt ccggcgggaa aacggcaatc gtaccgcgca aaatcaaata
     2821 ctgcaaacaa ccagcagggc ttcagggctt atagagcttc ttgatcgc
//
LOCUS       DAOMGY010000145         2855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_37870_length_2855_cov_2.532857, whole genome shotgun sequence.
ACCESSION   DAOMGY010000145 DAOMGY010000000
VERSION     DAOMGY010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37870_length_2855_cov_2.532857"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLR57_03095"
     CDS             <1..660
                     /locus_tag="PLR57_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter A family member"
                     /protein_id="HQQ40471.1"
                     /translation="VREGELFALLGVNGAGKTTTIKMLAGLALPTSGDALLLGESIAK
                     DPQTAKQFNGISPQETAIARNLTVLENLELMARIYGLERAEARKRALELLERLSLEEV
                     AKSRAKTLSGGMQRRLSIAMALISEPKVLFLDEPTLGLDVLARRELWKLVLSLKTRST
                     ILLTTHYLEEVEALSDRVGVMNKGKLIAVGTVQELMERTKSKTFEDAFVLLATETEAI
                     A"
     gene            657..1397
                     /locus_tag="PLR57_03100"
     CDS             657..1397
                     /locus_tag="PLR57_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40472.1"
                     /translation="MNGTRAFASRTVKELLRDPLNLAFGLGFPLVLLLLMTAIQANIP
                     VSIFELDTLTPGVAIFGLSFISLFSSTLISKDRTTSFLMRLFASPLSASGFITGYTMP
                     LLPMALVQTVVCYTCAAALGMQMTLNVLLAIVTTIPAALLFIGIGLLCGSVFNDKQVG
                     GICGALLTNVSAWLSGTWFDLNLVGGVFKKIAYALPFAHAVEAGRAAVAGDYGAIMPH
                     LWWVIAYAAVVFALAVIVFRKKMHRDNI"
     gene            complement(1584..2327)
                     /locus_tag="PLR57_03105"
     CDS             complement(1584..2327)
                     /locus_tag="PLR57_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQQ40473.1"
                     /translation="MKRTIGALSKLSGVSIRALHYYDEIGLLHPSEIADSGYRYYDEK
                     AVERLWQILFYRELDFPLSDIAEILASPEFDRARALEEHRTLLLQRRERLDGLITLVS
                     NALKGETAMEFKPFDTSKIDELRDQYAEEAKARWGNTEAYRESARCTAKYTDEEFARI
                     QERSAEVTRGFAALIGTDPKDSRVQALVKEWQALITESYYVCTDEILAGLGEMYVADE
                     RFTKNIDQFGEGTAKLMSEGIAYYCANKK"
     gene            2459..>2855
                     /locus_tag="PLR57_03110"
     CDS             2459..>2855
                     /locus_tag="PLR57_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQQ40474.1"
                     /translation="MIGFMKQRELLDFLNALECLKTNGRHSYTCGGIQETVAAHSWRL
                     AVFAMLILPEFPEIDGDKLIRMCLIHDFGEAITGDIPSFLKTDANEETETDAVGALIR
                     RLPEPQQGVFRELFAEMDAMQTKEARLYKA"
BASE COUNT          583 a          783 c          831 g          658 t
ORIGIN      
        1 gtgcgcgagg gggagctctt cgccctgctc ggcgtgaacg gcgcgggcaa gaccactacg
       61 atcaaaatgc tcgcggggct ggcgctgccg accagcggag atgcgctttt gctgggcgaa
      121 agcattgcaa aggacccgca gactgccaag cagttcaacg gtatttcccc gcaggagacc
      181 gccattgcgc gtaacctcac cgtgctggag aacctggagc tcatggcgcg catctacggt
      241 ctggagcgcg ctgaggcgcg taaaagagcg ctggagctac tcgaacgcct ctctcttgag
      301 gaagtggcga agagcagggc aaagacgctc tccggcggca tgcagcggcg gctcagcatc
      361 gcaatggcgc tgatctccga acccaaagtt ctgtttctcg acgagccgac gctggggctg
      421 gacgtgctgg cgcggcgcga gctctggaag ctcgtgctct cactcaaaac ccgctcgacg
      481 atccttctga ctacgcacta cctcgaagag gtagaagcgc tttccgaccg cgtgggtgtg
      541 atgaacaagg gtaaactcat agcagttgga acggttcagg agctcatgga gcggacaaag
      601 tcaaaaacct ttgaggacgc tttcgtgctt ctcgcaacgg aaacggaggc gattgcatga
      661 acggcacaag agcgtttgcc agccgcacgg ttaaggaact cctgcgcgac ccgctgaacc
      721 tcgctttcgg gctgggattt ccgctggttc tgctgctgct catgaccgcg attcaggcga
      781 atattcccgt aagcattttc gagctggaca cccttactcc gggcgttgct atcttcgggc
      841 tttcgtttat ctcgctgttt tcctcgaccc tgatcagcaa ggaccggacg acttcgtttc
      901 tgatgcggtt gtttgcctct ccgctgagcg cttccggttt cataaccggt tacacgatgc
      961 cgcttctgcc catggcgctt gtacagacgg tggtttgcta tacctgtgcc gccgctctcg
     1021 gcatgcagat gacgctgaac gtgctcctcg ccattgtgac cacgattccc gcggcgctgc
     1081 tctttatcgg catcggcttg ctctgcggca gcgtttttaa cgataagcag gtgggcggta
     1141 tttgcggcgc gctgttaacc aacgtcagcg catggctcag cggaacctgg ttcgatttga
     1201 accttgtcgg cggggtgttt aagaagattg cctacgcgct gccctttgcg cacgcggtgg
     1261 aagccggacg cgcggcggtg gcgggggact acggcgcgat tatgccgcat ctttggtggg
     1321 ttatcgcgta cgcggctgtc gtgttcgcgc tggcggtgat cgtcttccgc aagaagatgc
     1381 acagggacaa tatttaaggg aagtacagca aaaggcgcgg gtgaccgcgc cttttaagca
     1441 actcttcttt tcttaaaaga aaagaagcaa aagaacgatt ctcatctttt tcttgaaaga
     1501 aaaagaagca aaaagaactt ttgttactag ttgagctaaa aagtggggtt tccaaagggg
     1561 cggacgcccc tttggccgtt cccttatttt ttattcgcgc agtagtacgc aattccctcg
     1621 ctcatgagct tggcggtgcc ttcgccgaac tggtcgatgt tctttgtgaa ccgctcgtcg
     1681 gcaacgtaca tttcgccgag tcccgcaagg atttcatccg tgcagacata gtagctttcg
     1741 gtaatcagcg cctgccactc cttgacgagc gcttgaacgc ggctgtcttt cggatcggtg
     1801 ccgatcagcg cggcaaaccc gcgcgtaacc tccgcggagc gctcctgaat gcgggcaaac
     1861 tcttcatcgg tgtatttcgc agtacagcgc gcgctctcgc ggtaagcttc cgtattgccc
     1921 cagcgggctt tcgcctcttc ggcgtactga tcgcgcagtt cgtctatttt agacgtatcg
     1981 aatggtttga attccattgc tgtttctcct ttcaacgcat tggaaacgag tgtaatgagc
     2041 ccgtccagcc gttcgcgcct ctgcaaaagc agcgttcggt gttcctccag cgcgcgtgca
     2101 cggtcgaact ccggcgaagc gagaatttcg gcaatgtcgc tcagcggaaa atccagttcc
     2161 cggtaaaaca ggatttgcca gagccgttca actgccttct cgtcgtagta ccggtagccg
     2221 gagtccgcga tttcgctcgg gtgcagaagc ccgatctcat cgtaatagtg cagcgcgcgg
     2281 atggaaacgc ccgaaagctt gctcagcgcg ccgatggtac gtttcatgtt cactcacctc
     2341 ccgataagta tggtaaacca taccgtaacg tcagagtcaa gaactttttt gcgttgaata
     2401 taaaaatcgc aatgtgtttt tttactgccg gtcatttgcg ggcgggagga tttgtggtat
     2461 gatcggattt atgaagcaaa gagaattgtt ggacttttta aacgcgctgg agtgcctcaa
     2521 aaccaatggc aggcacagct acacctgcgg agggatacag gagaccgtcg ccgcgcattc
     2581 ctggcggctc gcggtgtttg ctatgctgat tctgccggag tttccggaga tcgacgggga
     2641 caagctcatc cgcatgtgcc tgattcacga cttcggcgag gcgattaccg gggacatccc
     2701 gtcgtttctc aaaacggacg ccaacgagga aacggaaacg gatgccgtcg gcgcactgat
     2761 ccggcggctg ccggagccgc agcagggcgt gtttcgcgag ctatttgccg agatggacgc
     2821 catgcagacc aaagaagcgc ggctctataa agcgc
//
LOCUS       DAOMGY010000146         2846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_38027_length_2846_cov_3.456467, whole genome shotgun sequence.
ACCESSION   DAOMGY010000146 DAOMGY010000000
VERSION     DAOMGY010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38027_length_2846_cov_3.456467"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLR57_03115"
     CDS             complement(<1..261)
                     /locus_tag="PLR57_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009661230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ40475.1"
                     /translation="MSELRVSVVGAGLAGCEAAYQLAKRGYCVDLYEMKPEKKSPAHK
                     SDALCELVCSNSMRSDRVENAVGLLKAEMRLLGSLVIRIADET"
     gene            complement(258..2372)
                     /gene="topA"
                     /locus_tag="PLR57_03120"
     CDS             complement(258..2372)
                     /gene="topA"
                     /locus_tag="PLR57_03120"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQQ40476.1"
                     /translation="MSDTLVIVESPAKAKTIGKFLGSKFKVVASNGHVRDLPKSQIGV
                     KIDQGFEPKYITLRGRGDVLERIRKEAKAADKVFLATDPDREGEAISWHLAQVLNLDE
                     KSKCRIEFNEITATAVKNSIKGARAIDMRLVNAQQARRILDRLVGYEISPILWRKVRK
                     GLSAGRVQSVATRIICDREMEIRDFIPEEYWNLSVKLRDVAGKKSFEAKYFGEEGKKR
                     DLHTEAETNAVLERIKSGLFVITDVKDGVKTRHPAPPFTTSSLQQEASRKLGFTTKRT
                     MMIAQQLYEGIELGTKGAVGLISYIRTDSVRVASEAQAAARSFVEKRFGKDYIPEKPN
                     FYKGRAGAQDAHEAIRPTGVDLEPAGIKDHLTSEQYRLYKLIYERFLASQMSDAVYAT
                     TQVTLDCEGETFRANGTRTMFDGFTIVYTEGRDDEQEKESSLPEVKVDEHYKAEKISS
                     EQKFTQPPAHYTEATLVKALEEKGIGRPSTYSPTISTIVERGYIAREKKQLIPTELGF
                     VVNRFMLENFDEIVDVQFTAMMETQLDEVEEDKVGWADVLGKFYGPFHEKVEVALEKA
                     PPEKIPDEVSDIVCEKCGAMMVYKMGRFGKFLACPNFPTCRNTKAIVDKIDVPCPKCG
                     AALIKRKSKKGKAFYGCEKYPECDFVSWDKPTKMACPKCGGMMVQKIGQNGSYIACMN
                     KECGYIHRAVKKGKEEQEGEAE"
     gene            complement(2391..>2846)
                     /locus_tag="PLR57_03125"
     CDS             complement(2391..>2846)
                     /locus_tag="PLR57_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQQ40477.1"
                     /translation="HGTLVVEAAERSGTSITAGFAHDQGRDVFAVPGRIGDEMSLGPN
                     GMISRGEAKAVLSAEDILGEYSCLHAPAETLPEAETVSVLTLSEEQQSICRALKNGGF
                     SFDELCEMLSLPIGVLNSCLTELQFLGIMKQLPGRMYALDACRVRLKDN"
BASE COUNT          497 a          853 c          792 g          704 t
ORIGIN      
        1 cgtctcgtcc gcaatgcgga tcacgagcga gccgagcagg cgcatctccg ccttgagcag
       61 cccaacggcg ttttcaacgc ggtcggagcg catggagttg ctgcatacga gttcgcacag
      121 cgcgtcgctt ttatgcgcgg gcgatttctt ttcgggcttc atttcgtaaa ggtcgacgca
      181 gtagccgcgc tttgcgagct ggtacgccgc ttcgcatccc gcgagccccg cgccgacgac
      241 gctgacccgc agttcgctca ttcggcttcg ccttcctgtt cttccttgcc ctttttcacc
      301 gcgcggtgaa tatagccgca ctctttgttc atgcaggcaa tgtagcttcc gttctgaccg
      361 atcttctgaa ccatcatgcc gccgcacttg gggcaggcca tcttggtggg tttgtcccag
      421 gagacgaaat cgcactccgg atatttttcg cagccgtaga acgccttgcc ttttttgctc
      481 ttgcgtttga tgagcgccgc tccgcacttg gggcacggca cgtcgatttt atccacaatc
      541 gcctttgtgt tccggcaggt gggaaaattc gggcaggcaa gaaacttgcc gaagcgcccc
      601 attttataca ccatcatcgc gccgcacttt tcgcacacga tgtccgaaac ctcgtccgga
      661 atcttttccg gcggcgcttt ttccaaagcg acttccacct tttcgtggaa cgggccgtag
      721 aacttaccca gcacgtccgc ccagccgact ttgtcctctt ccacctcgtc gagctgcgtt
      781 tccatcatgg ccgtaaactg tacgtctacg atttcgtcga agttctcgag catgaagcgg
      841 ttgaccacaa agccaagctc ggtgggaata agctgcttct tttcgcgcgc aatatagccg
      901 cgctccacga tggtggagat cgtcggcgaa tacgtgctgg ggcgtccgat gcccttttct
      961 tccagcgcct tgaccagcgt cgcttccgtg tagtgcgccg gaggctgggt aaacttttgc
     1021 tcgctggaaa tcttttccgc tttgtagtgc tcgtccacct ttacttccgg cagagagctt
     1081 tctttttcct gctcgtcgtc gcggccttcg gtgtagacga tggtaaagcc gtcgaacatc
     1141 gtgcgcgtgc cgttggcgcg gaaggtttcg ccttcgcagt ccagcgtcac ctgcgtggtg
     1201 gcatagaccg cgtcgctcat ctggctggcg agaaagcgct cataaatcag cttatacagc
     1261 cggtactgct cgcttgttaa atgatccttg atgccggcgg gttcgagatc cacacccgtc
     1321 gggcggatgg cttcgtgcgc gtcctgcgca cccgcgcggc ccttgtaaaa gttcggcttt
     1381 tcagggatgt agtccttgcc gaatcgtttt tcaacgaacg aacgcgccgc tgcctgcgct
     1441 tcgctggcaa cgcggacgga gtccgtgcgg atatagctga tcaagccgac cgcgcccttg
     1501 gtgccgagct cgatgccctc gtagagctgc tgggcaatca tcatggtgcg cttggtggta
     1561 aagccgagct tgcgggaggc ttcctgctgg aggctgctgg tggtaaacgg cggcgcggga
     1621 tggcgcgttt ttacgccgtc cttgacgtcg gttatgacga acagcccgct cttgatgcgc
     1681 tccagaacag cgttggtttc cgcttcggta tgcaggtcgc gctttttgcc ttcctcgcca
     1741 aagtacttgg cttcgaagct ctttttgccc gccacgtccc gcagtttaac ggagaggttc
     1801 cagtattctt cgggtatgaa gtcccgaatc tccatttcgc ggtcgcagat aatgcgggtg
     1861 gcaaccgact gcacgcgtcc ggccgaaagg cccttgcgca cttttcgcca gagaatgggg
     1921 ctgatttcgt agcccacaag gcggtcgaga atgcgccgcg cctgctgggc gttgacaagg
     1981 cgcatgtcga ttgcgcgcgc gcctttgata gagttcttca cagcagttgc ggtgatttcg
     2041 ttgaactcga tgcgacactt gcttttttca tctaaattga gcacctgcgc cagatgccag
     2101 ctgattgctt ctccctcgcg gtcagggtcg gtcgcgagaa aaactttgtc cgcggccttt
     2161 gcttccttgc gaatgcgttc gagaacatcg ccccgtccgc ggagcgtgat atatttcggt
     2221 tcgaagccct ggtcgatttt aacgccgatc tggcttttgg gcagatcgcg cacatggccg
     2281 ttcgaggcca cgaccttgaa cttgcttcct aaaaattttc cgatcgtttt tgcctttgca
     2341 ggcgactcca cgatcaccag agtatccgac atagcggttc ctcccgtcgg ttagttgtcc
     2401 ttgagccgca ctctgcacgc gtccaaagcg tacattctgc ccggcaactg cttcattatt
     2461 cccaaaaact ggagttctgt caagcatgaa ttcaaaacgc cgatcggaag cgacagcatt
     2521 tcgcagagtt cgtcaaagga aaatccgccg tttttgagcg cgcggcagat gctctgctgc
     2581 tcctcgctca acgttaaaac ggacacggtt tcggcttccg gcaaggtttc ggcgggcgcg
     2641 tgcagacagc tgtattcgcc cagaatatcc tccgccgaga gaacggcttt tgcttccccg
     2701 cgcgagatca tgccgttggg gccgaggctc atttcatcgc cgatgcgccc gggaacggca
     2761 aacacgtccc gcccctgatc gtgcgcaaaa ccggcggtaa tggaagtgcc gctgcgttcc
     2821 gccgcctcca cgacgagagt cccgtg
//
LOCUS       DAOMGY010000147         2842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_38111_length_2842_cov_3.335845, whole genome shotgun sequence.
ACCESSION   DAOMGY010000147 DAOMGY010000000
VERSION     DAOMGY010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38111_length_2842_cov_3.335845"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLR57_03130"
     CDS             <1..493
                     /locus_tag="PLR57_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HQQ40478.1"
                     /translation="IVCQYWRPFLAGGKHNAAHYANAGERVIGSDFFHMYFDYPYAAT
                     PLKKTFHYEPVLRGVKMSARANFTGMECAVWTEWIDTEEKLFFNMLPRLAAAAEAGWS
                     KQRRNYRAFVGKLPSHYALYDRLGLMYAKDAEKQKPFWKQLKTIKTFLTQNTHVELPG
                     GGV"
     gene            542..1060
                     /locus_tag="PLR57_03135"
     CDS             542..1060
                     /locus_tag="PLR57_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HQQ40479.1"
                     /translation="MNEVLKSIQTRRSVRSFLPKQVEPELLDALLEAGTYAPTGSGKQ
                     SPIIVAVQDPATVKQLSRMNAAVFGGTSDPFYGAPTILVVLADKTKRTYLLDGAAVLT
                     TLLNAAHALGLGSCWIHRAKEEFESAEGKALLQQWGVEGDYEGIGHVALGYAAQATGE
                     PAPRKSDYIVKV"
     gene            1112..2389
                     /locus_tag="PLR57_03140"
     CDS             1112..2389
                     /locus_tag="PLR57_03140"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQQ40480.1"
                     /translation="MAAQKNEEFGVIRDCVDSFHRVTGLGALVCAPEGNVLFETGRHC
                     RACTLCANLGQPEDVCVVARAQAWREAERFGGKYIYACPMGLTCIATPVQTELDLVAD
                     ITVGPFLMVDEEDFVTCELESRTQDEEKLHLARESLQTIPRVAPEQAEPIARQLFLSV
                     GGTGKSFRIGDLLARQGASSLMGSLSDFTEREKQEENPASAYPILIERDFLEAIAAYD
                     RARAEELLNELLGHIFFLTGGDFSRIRARILELLVLSSRAAIEAGADETQILALCEEC
                     VGLMGSIYDVDKLCYWLTGALKRFFGCLFDRVSGAEQNPMPRVLAYLRRNSARRITLE
                     EAAEQAHLSPSYFSRMFRARTGKTFSDYLTRLRVEHAKRLLKQPELDMAQIAAQTGFY
                     DQSHFTRAFKSVTGYTPGVFRRRRERGFDELKE"
     gene            complement(2473..>2842)
                     /locus_tag="PLR57_03145"
     CDS             complement(2473..>2842)
                     /locus_tag="PLR57_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325234.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HQQ40481.1"
                     /translation="AILGTVKGDLHDIGKNLVRLMMEGKGLEVIDLGTDVPPEVFVQT
                     AIDSGCQVICCSALLTTTMYAIKDVIAAMKAAGIRDQVKIMVGGAPITQTFCDQIGAD
                     CYMPDATSAAVAAVNLCQAS"
BASE COUNT          574 a          804 c          903 g          561 t
ORIGIN      
        1 catcgtctgc cagtactggc ggccgttcct cgcgggcgga aagcataacg ccgcgcatta
       61 tgccaacgcg ggcgaacggg tgatcggaag cgatttcttc catatgtatt tcgactatcc
      121 ctatgcggca acgccgctga agaagacgtt tcattacgag ccggttctgc gcggagttaa
      181 aatgagcgcc cgcgcaaact ttaccggcat ggagtgcgct gtttggacgg aatggatcga
      241 tacggaagaa aagctgtttt tcaacatgct gccgcgcctt gccgccgcgg cggaagcggg
      301 atggtcaaag cagcggagga actaccgcgc gtttgtcgga aaactccctt cgcattacgc
      361 gctgtatgat cgactggggc tgatgtacgc aaaggatgcg gaaaagcaga agccattttg
      421 gaagcagttg aagactatta aaacgtttct tacgcagaat acgcatgtcg aactgcccgg
      481 cggcggggta taataaagaa ggacagtaat cagcgaactg attctacgga ggtatttttc
      541 gatgaatgaa gtgttaaagt cgattcagac gcgccgctcg gtgcgctcgt ttttaccaaa
      601 acaggtggaa cccgaactgc tggatgcgct gctggaagcg ggaacctatg cgcccaccgg
      661 cagcggcaag cagtcgccta taatcgtcgc ggtgcaggac cccgcaacgg tcaaacagct
      721 ttcgcgcatg aacgccgcgg tgttcggtgg cacaagcgac ccgttttacg gcgcgccgac
      781 gattcttgtc gtgctcgcgg ataagaccaa aagaacctat ctgctcgacg gtgcggcggt
      841 gctgaccacg cttctcaacg cggcgcacgc actcggcctt ggctcctgct ggattcaccg
      901 tgccaaagag gagttcgagt ccgcggaggg caaagcgctt ttacagcagt ggggagtcga
      961 aggggactac gaggggatcg gccacgtggc gctcggttac gcggcgcagg cgaccggcga
     1021 acccgccccg cgcaagagcg actatatcgt aaaagtttaa tccgctgagt cgggttcatc
     1081 ccgaccagag cggcggcagg gaggcgacgg catggccgcg cagaaaaacg aggagttcgg
     1141 cgtcattcgg gactgcgtgg actcgtttca ccgggttacg gggcttggcg cgctggtttg
     1201 cgcgccggag ggcaacgtgc tctttgaaac gggccggcat tgccgcgcct gcacgctctg
     1261 cgcgaatctg ggccagccgg aggacgtgtg cgtcgttgcc cgcgcgcagg cctggcgcga
     1321 agcggaacgc tttggcggca agtatatcta cgcctgcccg atgggactga cctgcatcgc
     1381 aacccccgtt cagaccgagc tggatcttgt cgcggacatc accgtcgggc cgtttctgat
     1441 ggtggacgag gaggattttg tcacctgcga gctggaatcc cgcacgcagg atgaggagaa
     1501 actgcatctt gcgcgggagt cgctccaaac gattccgcgc gtagcgcctg aacaggcgga
     1561 gccgattgcg cggcagctgt ttctttccgt cgggggaacg ggcaaatcct tccgcatcgg
     1621 cgaccttttg gcgcggcagg gtgcgagcag cctgatgggc agcctttcag atttcaccga
     1681 gcgtgaaaag caggaggaaa atcccgcttc ggcgtacccg atcctcattg aacgcgactt
     1741 tctggaggcg attgccgcct acgaccgcgc acgcgcggag gaactgctca acgaactgct
     1801 cgggcatatc ttctttttga ccggcggcga cttttcgcgc atccgcgcgc gaatactcga
     1861 actgctcgtg ctgagctccc gcgcggctat cgaagcggga gcggatgaaa cgcagattct
     1921 cgcgctctgc gaggaatgcg tgggtctgat gggctcgatc tacgacgtgg ataagctctg
     1981 ctactggctt accggcgcgc tgaaacgctt cttcggctgc ctgttcgacc gcgtttccgg
     2041 cgcggaacaa aaccccatgc cgcgcgtgct ggcgtacctg cgtcgcaaca gcgcccggcg
     2101 catcacactg gaagaagcgg cggagcaggc gcatctttcg ccttcgtact tcagcaggat
     2161 gttccgcgcg cgaacaggca aaacgttttc ggactacctg acgcgcctta gagtggagca
     2221 cgccaaacgg ctgctcaaac agccggagct ggatatggcg cagattgccg cgcagacggg
     2281 gttctacgat cagagccact ttacccgcgc gttcaaaagt gtaacgggct acacgccggg
     2341 ggtatttcgg cggcggcgcg aacgaggatt tgacgaactg aaggagtagg ataaagcgat
     2401 ccgcctattc gggcggatcg ctttactgtt gaactcaaat tttgtccgaa ttatctcatc
     2461 gtccggttcg cttcagctcg cctgacagag attgacggcg gcgacggcgg cggaggttgc
     2521 gtccggcatg tagcagtccg cgccgatctg gtcgcagaag gtttgcgtga tcggagcgcc
     2581 gccgaccatg atcttgacct gatcgcggat gcccgcggcc ttcatggcgg cgataacgtc
     2641 cttgatggcg tacatcgtgg tagtcaaaag cgccgagcag cagatgacct gacagccgct
     2701 gtcgattgcg gtctggacga agacttccgg cggaacgtcc gtgccgaggt cgatgacttc
     2761 gagtcccttg ccctccatca tgaggcggac gaggttcttg ccgatatcgt gcaggtcgcc
     2821 cttcacggtg cccagtatcg cc
//
LOCUS       DAOMGY010000148         2828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_38383_length_2828_cov_4.079697, whole genome shotgun sequence.
ACCESSION   DAOMGY010000148 DAOMGY010000000
VERSION     DAOMGY010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38383_length_2828_cov_4.079697"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLR57_03150"
     CDS             <1..356
                     /locus_tag="PLR57_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40482.1"
                     /translation="GGPEELPEPASYSPAPARVAPPSQPQRQFERTSSDFSEPVDKAS
                     RSSGRSIRDEVFSESYRPRKKKKHRSGAFSIILLILVCLILLWVVAGVAMTMKWIPEY
                     DLGYTWFNLNIFKLF"
     gene            complement(472..741)
                     /locus_tag="PLR57_03155"
     CDS             complement(472..741)
                     /locus_tag="PLR57_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HQQ40483.1"
                     /translation="MTGAQRAEFRKAANTLKPIFQIGKEDIGENLIKSVDEALTKREL
                     IKLAVLETSSLTAKEAAAVLAERTGAEMIQCIGRKFVLYRQKPEE"
     gene            complement(954..2696)
                     /locus_tag="PLR57_03160"
     CDS             complement(954..2696)
                     /locus_tag="PLR57_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HQQ40484.1"
                     /translation="MDKVTVKVNGIEAKVPANATILEAAHEAGIQIPTLCYLKKINAI
                     AACRVCLVQATGVRGHAAACVTQVAEGMEITTNTPELRAARKTTLELILSNHRMDCLS
                     CDRNGDCELQSLAREYGVDQYKYAGSEDLRPQYERSAPHLVRDNSKCILCRRCVAVCK
                     HNQHVAVIGTNERGFDTHVGSAFDMPLAETTCINCGQCISVCPTGALTERDDTAKVWE
                     ALADKTKHVIVAPAPSVRVQLGECFGMPIGTNVEGKLVAALRRLGFEKVFDVDFAADV
                     TIMEEGTELLDRLKNGGKLPLITSCSPGWIKFCEHYYPDMIENLSSCKSPQQMFGALV
                     KTYYAEKEGIDPKDIFVVSIMPCTAKKFECTRDDENAAGVPDVDISLTTRELGNMIKR
                     AGIMFTDLKDEDFDPMMGVASGAGHIFGATGGVMEAALRTVAEIVTGKPLDDLEFKAV
                     RGVEGCKEAEYDLAGTKVRVAVTSGLENAHQLLEMVRKGEADYHFIEVMACPGGCVNG
                     GGQPHQPGSVRNFIDLRAERAKGLYGEDKCMTLRKSHENPFVKELYDTYLEKPGSHKA
                     HKILHTTYVKRKVY"
     gene            complement(2709..>2828)
                     /locus_tag="PLR57_03165"
     CDS             complement(2709..>2828)
                     /locus_tag="PLR57_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQQ40485.1"
                     /translation="CPVSAITGTVKQPHVIDQQKCIKCGVCMENCKFGAISKK"
BASE COUNT          542 a          822 c          801 g          663 t
ORIGIN      
        1 acggcgggcc ggaagaactg ccggaaccgg cgtcgtactc tccagcaccc gcgcgcgtcg
       61 ccccgccttc gcagccgcag cggcagttcg aacgcaccag ttccgatttc tccgagccgg
      121 tggacaaggc ctcgcgttcc agcggacgct ccatccgtga cgaagtattc agcgagtcct
      181 atcgtccacg caaaaagaaa aagcaccgca gcggcgcatt cagcatcatc ctgctgattc
      241 tcgtatgcct gattctgctc tgggtcgtcg caggggtcgc catgaccatg aagtggatcc
      301 ccgagtacga tctgggttac acctggttca acctgaacat cttcaagctc ttttagactc
      361 ttcttttctt aaaagaaaag aagcaaaaga atacctcttt acaaacaaag aacttcccaa
      421 tcaggaagtt ctttttgttt tactttttct ttcaagaaaa agtaaaatac cctactcctc
      481 cggcttctgc cggtacagca cgaacttgcg accgatgcac tgaatcatct ccgcgcccgt
      541 gcgttcggca agcaccgccg cggcttcctt tgccgtaagc gagctcgtct ccagtacggc
      601 aagcttgatg agttctcgct tggtcagcgc ttcgtccacg ctcttgatta agttctcgcc
      661 gatatcctct ttgccaatct ggaaaatcgg cttgagggta ttcgccgctt ttcgaaattc
      721 tgcgcgctgc gctccggtca tggtatcgta ctcctctccc gcaagagcgg gtttgttttt
      781 tatttttgcg cttgattata acacgcgatt ccaaaaaaag cgagaattga ccgggaaaac
      841 agcgattcac tttttcttga aagaaaaagt aaacaaaaag aacttcctct ttgtcggtac
      901 aagaaaaatc cccgcacggt atgtgcgggg atttttgatg cagccttttg gaattaatag
      961 accttcctct tgacgtatgt cgtgtggagg atcttatgcg ccttgtggga gccgggcttc
     1021 tcaaggtagg tgtcgtacag ctccttgacg aaggggtttt catggctctt gcggagcgtc
     1081 atgcacttat cttcgccgta cagacccttg gcacgctcgg cacgcaggtc gatgaagttg
     1141 cgaacgctgc cgggctgatg cggctgaccg ccgccgttga cgcagccgcc ggggcaggcc
     1201 atgacttcga tgaagtgata gtccgcttcg cccttgcgca ccatttcgag cagctgatgc
     1261 gcgttttcaa ggccgctggt aaccgcaacg cgcaccttgg tgcccgcgag gtcgtattcc
     1321 gcttccttgc agccttccac gccgcggacg gctttgaatt ccaagtcgtc caggggcttg
     1381 ccggtcacga tttccgcaac ggtgcgcaga gccgcttcca taacgccgcc ggtcgcgcca
     1441 aagatatggc ccgcgccgga cgcaacgccc atcatcgggt cgaagtcttc gtctttgagg
     1501 tcggtgaaca tgatgcccgc gcgcttgatc atgttgccca gttcgcgcgt agtgagcgag
     1561 atatcgacgt ccggcacgcc cgccgcattt tcgtcgtcgc gggtgcactc aaacttcttg
     1621 gccgtgcagg gcatgatgga aaccacgaag atatccttcg ggtcgatgcc ttctttttcg
     1681 gcgtagtagg ttttcaccag cgcgccgaac atctgctgcg ggctcttgca gctcgagagg
     1741 ttctcgatca tatccggata gtaatgctcg cagaacttga tccagccggg cgagcaggaa
     1801 gtgatcagcg ggagcttgcc gccgttcttc aaacggtcga gcagctcggt gccttcttcc
     1861 ataatcgtca cgtccgcggc gaagtcaaca tcgaagacct tttcaaagcc gagacggcgg
     1921 agcgccgcaa cgagcttgcc ttccacgttg gtgccgatcg gcatgccgaa gcattcgcca
     1981 agctgaacgc gtacggaggg cgcaggagcg acgatgacat gctttgtctt gtccgccagc
     2041 gcttcccaaa ccttcgcggt atcgtcgcgc tcggtcaaag cgcccgtcgg gcagacggag
     2101 atgcactggc cgcagttgat gcaggtggtt tccgccagag gcatatcgaa cgcgctgccg
     2161 acatgcgtat cgaacccgcg ctcgttggtg ccgatcacgg ccacgtgctg gttgtgtttg
     2221 cagacggcga cgcagcggcg gcagaggatg cacttgctgt tgtcgcgtac cagatgcggc
     2281 gcgctgcgct cgtactgggg acggagatcc tcgctgccgg catacttgta ctgatccacg
     2341 ccgtactcgc gggcgagaga ctgcagttcg cagtcgccgt tgcggtcgca gctaaggcag
     2401 tccatgcggt ggttgctcaa aatgagctcc agggtggtct tccgcgcggc gcggagttcc
     2461 ggcgtgttgg tggtaatctc catgccttcg gcaacctggg tgacgcaggc ggccgcgtgg
     2521 ccgcgcacgc cggtggcctg cacaaggcag acgcggcagg ccgcgatggc gttgatcttc
     2581 ttgaggtagc acagcgtagg aatctggata cccgcttcgt gcgccgcttc caatattgtt
     2641 gcgttggcgg gaacctttgc ttcgatcccg ttgactttta ctgttacttt atccatgttc
     2701 ctgcctcctt actttttgga aatggcgccg aacttgcagt tctccatgca aacgccgcac
     2761 ttgatgcact tctgctggtc gatgacatgc ggctgcttga cggtgccggt gatggcgctg
     2821 acggggca
//
LOCUS       DAOMGY010000149         2822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_38505_length_2822_cov_3.121431, whole genome shotgun sequence.
ACCESSION   DAOMGY010000149 DAOMGY010000000
VERSION     DAOMGY010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38505_length_2822_cov_3.121431"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="PLR57_03170"
     CDS             <1..1638
                     /locus_tag="PLR57_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQQ40486.1"
                     /translation="PTIQNTVQTTLSTWDSYPSLADTSKSLLVETGEDGTVTETVEPQ
                     AASVVLDYHTGELRAVVGGRNEPTIRKGINRATSYTEVGSSIKPLAVYGPALDLGFSP
                     ASIVQNMDGPIQGWTTEKGYPSGGLDSRYGPVTFRRALMSSLNVGAARVLMDDVTPAI
                     SAEYLYQLGATESKINVDGAGLALGTSGLTPIQMAAAYGTIANGGVYLEPLSFTKVLD
                     DAGNVILDADEVRDSHRVFQESTAYLLVDMMTDAVKSGTGTKAKITDMTVAGKTGTNS
                     DYASVYFAGITPYYASSLWVGHDYPANKLKKGSSGGDEAAPLWQAYMSKIHEGLENKS
                     IIDTDPTSLGLVKRTVCSVSGLLATDACKDDEDHKPVTDWFLASNAPTEYCDMHVTLD
                     LCSAENAIATELCPSELVTRTSFILIRPDSMFYQFEDEYLLKAFPNAVRTELSTDDFI
                     ASMKTCTVHSDGSLSIFELKINAQDLMQTVTDYLNTAVGIPMEMSDKLKNDVAALEVA
                     LTGYEYSVIEPLYTTLQTDFTALQNYMATQTQGDNGG"
     gene            1631..2284
                     /locus_tag="PLR57_03175"
     CDS             1631..2284
                     /locus_tag="PLR57_03175"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQQ40487.1"
                     /translation="MADEQTKKENAEVSLQLELLYAFERERLGRLAALDQPQGEYAHP
                     VFGEGSAHAKVLLIGEAPGAEETKHGRPFVGKAGKQLDELFHLFGISRENAYITNVVK
                     YRPCVRSPRSVRNRTPVPSEVEASLPLLQKELELIRPDFLCTLGNTPLKAVYRLSGEK
                     PEVVGNVHGRQIPLTVGGVSFSLIPLYHPASGIYNRALIEVMREDAEKVHAAIANKT"
     gene            2351..>2822
                     /locus_tag="PLR57_03180"
     CDS             2351..>2822
                     /locus_tag="PLR57_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQQ40488.1"
                     /translation="MAQKVLIADDDPVVHESLGIYLKAEGFETVDAFDGVQALESITP
                     EVVLCVLDIMMPKMSGIEVCREIRKTSQLPVLMLTAKGEEIDRIVGLELDADDYIVKP
                     FSPREVVARIKAVLRRATEQPKSDSSVISYNGLTIDLKSYTVTLRGQPVICTPKE"
BASE COUNT          659 a          790 c          818 g          555 t
ORIGIN      
        1 ccgacgattc agaataccgt gcagaccacg ctgagcacat gggacagcta tccttcgctt
       61 gcggacacct ccaaatccct tctagttgaa accggcgagg acggaacggt aacggaaacc
      121 gtcgagccgc aggcagcgtc cgttgtgctg gattaccata caggcgaact ccgcgcggtc
      181 gtcggcgggc ggaatgaacc aacgattcgc aaaggcatca accgcgcaac gagctataca
      241 gaagtcggct cttcgattaa gccgcttgct gtttacggtc ccgcactcga tttgggcttt
      301 tcacccgcaa gcatcgtgca gaatatggac ggcccgattc aaggctggac cacggagaag
      361 ggatatcctt ccggcgggct ggattcccgt tacggaccgg taaccttccg ccgagcgctg
      421 atgagctcgc ttaacgttgg cgcggcgcgc gtgctgatgg acgacgtaac gccagccatc
      481 tctgcggagt acctttatca gcttggcgca accgaaagca agatcaatgt ggacggcgca
      541 ggtctcgcgc ttggtacttc cggcttaacg cccattcaga tggccgcggc atacggaact
      601 atagccaacg gcggggtcta tcttgaaccg ctgtcgttta ccaaggtgct ggacgatgct
      661 ggcaacgtca ttcttgacgc agacgaagtg agagattcgc accgcgtgtt ccaggagagc
      721 acggcgtatc ttttagtaga tatgatgacg gatgcggtca agtccggcac gggcaccaag
      781 gcaaagatta cggatatgac cgtcgccggc aagacgggca cgaactccga ctacgcgagc
      841 gtttattttg cgggcattac gccgtattat gcttcctcgc tctgggtcgg acacgattat
      901 cccgccaaca agctcaagaa gggttcttcc ggcggggatg aagcggcgcc gctctggcag
      961 gcgtatatgt ccaagattca cgaaggactg gagaataaat ccatcatcga cacggatccg
     1021 acgtcactgg gcttagtcaa acgcacggtc tgctccgttt ccggcctgct tgccacagac
     1081 gcctgcaagg acgacgagga ccacaagccc gtgaccgact ggttccttgc cagcaacgcg
     1141 ccaacggaat actgcgatat gcacgtgacg ctggatctct gctcggcgga gaacgcaatc
     1201 gcaaccgagc tttgcccgtc ggaactggtc acaaggacga gctttatcct gatacgtccg
     1261 gattccatgt tctaccagtt cgaggacgag tatttactca aagcgttccc caacgccgta
     1321 cggacggaac tttcaacgga cgattttatc gcctccatga agacctgcac cgtgcattcg
     1381 gacggctcgc tttcgatttt cgagctcaag atcaacgcgc aggatctgat gcagacggtg
     1441 acggactatc tcaacaccgc cgtcggcatc cccatggaga tgagcgacaa acttaaaaac
     1501 gacgttgcgg ctctggaagt agcgctgacc ggatatgagt actcggtgat tgagccgctg
     1561 tataccacgc tacagacaga ctttaccgcg ctgcagaact atatggcaac gcagacgcag
     1621 ggggacaacg gtggctgacg aacagacgaa gaaggaaaat gccgaagttt cgctccagct
     1681 tgaactgctg tacgcgtttg agcgggagcg ccttggcagg cttgccgcgc tcgaccagcc
     1741 tcagggcgaa tatgcacatc cggtctttgg cgaaggctcg gcacatgcaa aagtgctgtt
     1801 aatcggcgaa gcgccgggcg cggaagagac gaagcatggc cgtccgttcg tcggcaaggc
     1861 gggaaaacaa ctcgacgagt tgtttcatct gttcggcatt tcgcgcgaga acgcatatat
     1921 tacgaacgtg gtcaaatacc gtccctgcgt gcgctcacca agaagcgttc gtaaccgcac
     1981 gccggttcct tcggaagtgg aagcatcact gccgcttctg caaaaggaac tggagctgat
     2041 acggccggac tttctctgca cgctcggtaa cacgccgctc aaggccgtct accggctgtc
     2101 cggcgaaaag ccggaggtgg tgggaaacgt ccacggcagg cagattccgc tcacggtagg
     2161 cggcgtttcg ttttcgctga ttccgctgta ccatccggcg agcggcatct ataaccgcgc
     2221 gctgattgaa gtaatgcgcg aggacgcgga aaaggtgcat gcggcgattg cgaacaaaac
     2281 gtgacgctgt acgtttcaaa cgtgagtatg gtatactaaa gaataattca gcaacagcaa
     2341 ggagcgtatt atggcgcaga aagtattaat tgcggacgac gatccggtcg tacacgaatc
     2401 tctcgggatt tacctgaagg ccgagggctt tgaaacggtg gacgcgttcg acggcgtcca
     2461 ggcactggag agcattacgc cggaagtcgt gctctgcgtt ctggatatca tgatgcccaa
     2521 gatgagcggc atcgaagtct gccgagaaat tcgcaagacc tcgcagctgc cggtactcat
     2581 gctcaccgca aagggagaag agatcgaccg tatcgtgggt ctggagctcg acgcggacga
     2641 ctatatcgtc aagcccttca gcccgcggga agtggtcgcc cgcatcaaag cggtgctccg
     2701 ccgcgcaacc gaacagccca agagcgattc ttcggtgatt tcctacaacg gcctcaccat
     2761 cgacctcaag agctatacgg tgacgcttcg cggccagccg gtcatctgca cgccgaaaga
     2821 ga
//
LOCUS       DAOMGY010000150         2817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_38579_length_2817_cov_3.555033, whole genome shotgun sequence.
ACCESSION   DAOMGY010000150 DAOMGY010000000
VERSION     DAOMGY010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38579_length_2817_cov_3.555033"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PLR57_03185"
     CDS             <1..549
                     /locus_tag="PLR57_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ40489.1"
                     /translation="KQADVPGALAAYRKLTELSGKTVGIVGYGRIGQTLGKSCQALGM
                     NVLAYDIYKVPELECETMRYVEMDELLAKSDFVSLHVPSLPGQPLINAETLAKMKDGV
                     IIVNTSRGTNVDEAALLDALNSGKVYGAGLDVFAEEPAKNAALYTHPRVSSTPHIGAS
                     TKEAQKRIGAEIVDILVKYFNL"
     gene            948..>2817
                     /locus_tag="PLR57_03190"
     CDS             948..>2817
                     /locus_tag="PLR57_03190"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HQQ40490.1"
                     /translation="MFTEIIKRDGHKEPFNKNKIKSAIWKAAQAVNGTDEQLSERLTD
                     EVVRLAELRYAGTTPDVEGIQDLVEKVLIEAGHAKTAKAYILYREKRRGTREINALIG
                     ATIDMFGAYLDDRDWGVKENANMQRSVNGLNNYVREAFTKKYWLYEIYPIDICKAHES
                     GDAHIHDLGFFGPYCAGWDLRQLLTDGFGGVEGKVESKPAKHLRSFLGQIVNSTFTTQ
                     GETAGAQAWSSFDTYCAPFLYYDNMTFTQVCQCLQEFVFNINVPTRVGFQCPFSNLTF
                     DIRVPSTLKDEPVLIGGQMMDRTYGEFQEQMDLFNRAFCAVMLEGDAKGRVFTFPIPT
                     INITKDFDWSSPVVDDFMRITCKYGIPYFANYVNSDLSPEDAVSMCCRLRLDKRELRK
                     RGGGLFGSNPMTGSIGVYSINLPRIGYLATSKEDFFDRLRQIAVLGKTSLEIKRKIIE
                     QQTEQGLYPYSMHYLRNVKERSGEYWSNHFNTIGLVGMNECCLNFVGKDIGSPEGLAF
                     SLEVMHFLRALMIEFQEETGHSYNLEATPAEGTSYRLAKLDRERCPGIIQSGEDEPMY
                     TNSTQLPVGYTDDIFECCDLQDELQSLYTGGTVQHLYIGERIEDIETCKTVIRTLFE"
BASE COUNT          713 a          769 c          737 g          598 t
ORIGIN      
        1 aagcaggccg acgtgcccgg cgcgctcgcc gcgtaccgca agctcaccga gctctccggc
       61 aagaccgtcg gcatcgtggg ctacggccgc atcggccaga cgcttggcaa gagctgccag
      121 gcgctgggca tgaatgtgct ggcttacgac atctacaagg ttccggaact ggaatgcgaa
      181 accatgcgct atgtcgaaat ggacgaactg ctcgccaagt cggacttcgt ttctctgcat
      241 gttccgtcac ttcccggaca gccgctgatc aacgccgaaa cgctcgctaa gatgaaggac
      301 ggcgttatca tcgtcaacac gtcccgcgga accaacgtgg acgaagcggc cttgctggac
      361 gctcttaaca gcggcaaggt gtacggcgcg ggtctcgacg tattcgcaga agaacccgcc
      421 aagaacgcgg cgctctatac gcatccgcgc gtgagcagca cgccgcatat cggcgcttcc
      481 accaaagaag cgcaaaagcg catcggcgct gaaatcgttg atatccttgt aaagtatttc
      541 aatctgtaaa actgctaccg tctttcagcg ccggagaaag caacttctcc ggcgcttttc
      601 tattgtggtt tttgaagaaa acagtacaat atctagtaag tttacagttt gtacgataca
      661 aaatattgtg tccaattctg atttgaaata tgaaatcttt ataaacaaaa tacatgaaaa
      721 tacactgcat gcagtagcta tgcattacgt gttacaaggt tactcttgca aaactgccgg
      781 aaaagggcaa attttacgca gatttgatta atagaaagaa aaagctgtga attgtccaca
      841 tcttgtccac cgaatatatc aatatctggt atgcaagctc tcctattgac actagatata
      901 cgcgtagcta aactgaagat acgacacaac gaagcggggg cggtaatatg tttacagaga
      961 tcattaagcg cgacgggcac aaggagccgt ttaataagaa taagatcaaa agcgcgatct
     1021 ggaaagcggc gcaggcggtc aacggtaccg acgagcagct ctccgaacgg ctgacggacg
     1081 aagtcgtccg gcttgccgag ctgcgctatg ccggaaccac gccggacgtg gaaggcattc
     1141 aggatctcgt agaaaaagtt ctgatcgaag ccggacacgc caagaccgcc aaagcttata
     1201 ttctctaccg cgaaaagcga cgcggcacgc gtgaaatcaa cgctctcatc ggcgctacca
     1261 tcgatatgtt cggcgcttat ctggacgacc gcgactgggg cgtcaaggaa aacgcgaaca
     1321 tgcagcgcag cgtcaacggc ctcaacaact atgtccgtga ggcgtttacc aaaaagtact
     1381 ggctctacga aatatacccc atcgatatct gcaaggcgca tgaaagcggg gacgcgcata
     1441 tccatgacct cggcttcttc gggccatact gcgcaggctg ggatcttcgc cagcttttga
     1501 cggatggctt cggcggtgtt gaaggaaaag ttgaaagcaa gcccgcaaag cacctgcgtt
     1561 cgtttttggg gcagatcgtc aactccactt ttacgacgca gggcgaaacc gcgggcgcgc
     1621 aggcttggag ctcgtttgac acctattgcg ctccgttcct ttattacgac aacatgactt
     1681 ttacgcaggt atgccagtgc ctacaggaat ttgtattcaa tatcaacgtg ccgacacgcg
     1741 tcggcttcca gtgcccgttt tcgaacctga cgttcgatat ccgcgttccg tccaccctca
     1801 aggacgagcc tgttttgatt ggcgggcaga tgatggatcg cacctacggc gaattccagg
     1861 agcagatgga tctgttcaac cgcgcgttct gcgccgtcat gctggaaggc gacgccaagg
     1921 gcagggtctt cacgttcccg attccaacca tcaacattac aaaggatttc gattggtcga
     1981 gccccgttgt ggacgacttt atgcgcatca cctgcaaata cggcattccg tacttcgcca
     2041 actatgtcaa ctccgacctg tcgccggagg acgcggtttc catgtgctgc aggcttcgcc
     2101 ttgacaaacg cgagctcaga aaacgcggcg gcggcctttt cggcagcaac cccatgacgg
     2161 gaagcatcgg cgtttacagc atcaacctgc cgcgtatcgg ttatcttgca acatccaagg
     2221 aagacttctt tgaccgcttg aggcaaattg ccgtgctcgg caaaaccagc cttgaaatca
     2281 agcgcaagat catcgagcag cagacggaac aggggctgta cccgtattcc atgcactacc
     2341 tcagaaacgt caaggaacgc agcggcgagt attggtcgaa ccacttcaac accatcggat
     2401 tggtcggcat gaacgaatgc tgcctcaact ttgtcggcaa ggacatcggc agccccgaag
     2461 gacttgcgtt ctcgcttgaa gtcatgcact ttttacgtgc gctcatgatc gagttccagg
     2521 aggagaccgg gcacagctac aatctggaag cgacgcccgc ggaaggcaca agttaccgtc
     2581 ttgccaagct cgaccgcgaa cgctgccccg gcatcattca gtccggcgag gatgagccga
     2641 tgtatacgaa ctcaacgcag ttgcccgttg gctatacgga cgatatcttc gagtgctgtg
     2701 atttgcagga cgaacttcag tcgctctata caggcggaac ggtgcagcac ctctatatcg
     2761 gcgagcgcat cgaggatatc gaaacctgca aaaccgtcat ccgcacgctg tttgaaa
//
LOCUS       DAOMGY010000151         2802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_38886_length_2802_cov_3.403349, whole genome shotgun sequence.
ACCESSION   DAOMGY010000151 DAOMGY010000000
VERSION     DAOMGY010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38886_length_2802_cov_3.403349"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLR57_03195"
     CDS             complement(<1..630)
                     /locus_tag="PLR57_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009533597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HQQ40491.1"
                     /translation="MDYAKESLRLHKEWKGKIEVKATVPVATREDLSLAYTPGVAQPC
                     LEIQKDVELSYELTRRWNLCAVVTDGTAVLGLGDIGPEAGMPVMEGKCVLFKSFGDVD
                     AFPLCVKSKEVEDIVNTVYLISGSFGGINLEDISAPRCFEIERKLKEKCDIPIFHDDQ
                     HGTAVVTLAGLINALKVVGKKKSDVKIVINGAGAAAISVTKLLLSDGFKS"
     gene            complement(708..1820)
                     /locus_tag="PLR57_03200"
     CDS             complement(708..1820)
                     /locus_tag="PLR57_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HQQ40492.1"
                     /translation="MAETRPYISWDLIKNFMIDTFKAYGVPQEDAEICTDVLLESDRR
                     GIESHGCNRFKPIYLDRIDAGILYPVTNYEVVKETPTTLVADAHDGMGMVASYKAMKT
                     IIEKAKKYGMAMAAIRNSSHYGIAGYWASMAVNEGMIGITGTNARPSIAPTFGVENML
                     GTNPLTVGMPTDEDFPFMLDCATSISQRGKIEYYARSGKDTPAGMVIGRDGSAMTDSP
                     AILKGLTDGSAALAPLGGIGEDLAGYKGYGYATVVEILSAALQAGGFLKMCVDRDENG
                     NKKPYHLGHFFIVIDPEAFCGLETFKKISGEILRELRASEKAPGQTRIYTAGEKEHDV
                     WLERKEKGVPVGEAVQKEFIAIRDKKNLPYKFPFEK"
     gene            2191..2754
                     /locus_tag="PLR57_03205"
     CDS             2191..2754
                     /locus_tag="PLR57_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HQQ40493.1"
                     /translation="MQSILEQLQINPVIASVTSEDAFEAALHSECRVVFLLVGNILDI
                     GALTERVHEAGKLCVIHIDLIEGYSTKEIAVDALHKFTSAEGIISTRGTQIKRAKQLG
                     MTAIQRGFMLDSRSLSIFEAQIQTSKPDFVEILPGLLPKVITELKARLDVPIIAGGFI
                     HEKEDVIAAIQAGAMAVSASRPEIWSM"
BASE COUNT          568 a          751 c          808 g          675 t
ORIGIN      
        1 gctcttaaag ccgtccgaaa gcaggagctt ggtcacgctg atggcggcag cgccagcgcc
       61 gttgatgacg attttgacgt ccgacttctt tttgccgacg accttgagcg cgttgataag
      121 gcccgcgagg gtaacaaccg ccgtaccgtg ctggtcgtca tggaaaatcg gaatgtcgca
      181 cttttccttc agtttgcgct cgatttcgaa gcagcggggc gcggagatat cctcgaggtt
      241 gatgccgccg aagctgccgc tgatgaggta gacggtgttg acgatatctt cgacttcctt
      301 gctcttaacg cagagcggga aggcgtccac gtcgccaaag gatttgaaca gaacgcattt
      361 accttccata accggcatac ccgcttcggg gccgatatca ccgaggccga gcaccgcggt
      421 gccgtcggtg acgacggcgc agaggttcca gcggcgggtc agttcgtagc tgagttcaac
      481 gtccttctga atctccaggc agggctgcgc aacgcccggc gtgtaagcca gcgagaggtc
      541 ttccctcgtg gcaacgggaa cggtcgcttt gacttcgatt ttgcccttcc attccttgtg
      601 caggcggagg gactccttgg cgtaatccat attgattctc ctttatttat tcaaatgctg
      661 tccgcttggg gttcaatatt ttaccaggtg gttctcttca gcctgcgtta tttctcaaac
      721 gggaacttgt agggcaggtt cttcttgtcg cggatggcga tgaattcctt ctgcaccgct
      781 tcgccgaccg gaacgccctt ctccttgcgc tcgagccaga cgtcgtgctc tttttcgccc
      841 gcggtataga tgcgcgtttg tcccggcgct ttttcggaag cgcggagctc acgcagaatt
      901 tcgccggaga tctttttgaa cgtttcgagg ccgcagaacg cttccggatc gataacgatg
      961 aagaagtgcc cgaggtggta gggcttcttg ttgccgtttt cgtcgcggtc gacgcacatc
     1021 ttgaggaagc cgcccgcctg gagcgccgcg gagaggattt cgaccacggt cgcgtaaccg
     1081 tagcccttgt agcctgcgag gtcttcgccg atgccgccca gcggcgcgag agcggcggag
     1141 ccgtccgtaa ggcccttgag gatggcgggg ctgtccgtca tggcgctgcc gtcgcggccg
     1201 atgaccatgc ccgcgggggt gtccttgccg gaacgtgcgt agtattcaat tttgccgcgc
     1261 tggctgatgc tggtggcgca gtccagcatg aaggggaagt cttcgtccgt tggcatgccg
     1321 acggtgagcg ggttggtgcc aagcatattc tcaacgccga acgtgggcgc aatgctgggg
     1381 cgcgcgttgg tgccggtgat gccgatcatg ccttcgttga ccgccatgct agcccagtag
     1441 cccgcgatgc cgtaatgcga actgttacgg atggcggcca tagccatgcc gtactttttg
     1501 gctttttcga tgatggtttt catggccttg tagctcgcga ccatacccat gccgtcgtgc
     1561 gcatccgcaa cgagggtggt gggggtttcc ttgacgactt cgtagttcgt gacggggtag
     1621 aggatgcccg cgtcgatgcg gtcgaggtag atgggtttaa agcggttgca gccgtggctc
     1681 tcaatgccgc ggcggtcgct ttcgagcagg acgtccgtgc agatttccgc gtcttcctgc
     1741 ggaacgccat aagccttgaa cgtgtcgatc atgaagttct tgattaaatc ccaggagata
     1801 tacggtctag tttctgccat agtgtgaatc ctcctttagt gatgttggtt atattgaaat
     1861 gggaatctgc cgatggggca gaaaaaacgc cgggcaagta aaaaggagca cgcgcaacaa
     1921 acctgtgcgc cgctccaatc tccaaatctc aatggaactt ttgtacacgc ctatcatacc
     1981 cgcggctggc tggtttgtca atccattttg atcttttact tgcgttccgt cttggttttc
     2041 cttattctat tatacaagaa gtttttgatt atactattgc ttaacaagca aaggtcgaaa
     2101 ttcttttgct tcttttcttt taagaaaaga agagaatacc cgaaattctt ttgttaattt
     2161 ctttcaagaa aagaagagaa agcgagaaaa atgcagtcta ttctcgaaca actgcagatc
     2221 aacccagtaa tcgcctccgt caccagcgag gacgcgtttg aagccgcgct ccactccgag
     2281 tgccgggtgg tgtttctgct ggtgggcaac atcctcgata tcggcgcgct gaccgagcgc
     2341 gtacacgaag cgggcaagct ctgcgtcatc catatcgacc tgattgaagg ctactccaca
     2401 aaggagattg ccgtggacgc gctgcataaa ttcacgagcg cggagggcat catctccacg
     2461 cgaggaacgc agatcaagcg cgcaaaacag ctcggcatga cggcgattca gcgcgggttc
     2521 atgctggaca gccggtcgct cagcatcttt gaagcgcaga ttcaaaccag taaaccggac
     2581 tttgtggaga ttctgccggg acttctgccc aaggtgatta ccgaactgaa ggcgcggctc
     2641 gacgtgccca ttatcgcggg cggcttcatc cacgaaaagg aggacgtcat cgccgctatt
     2701 caggcaggcg caatggcggt ttccgcatcc cgccccgaaa tctggtcaat gtagaagcct
     2761 gtctggccaa aaccggaact acatagtaca tttacagcaa ac
//
LOCUS       DAOMGY010000152         2777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_39424_length_2777_cov_2.917340, whole genome shotgun sequence.
ACCESSION   DAOMGY010000152 DAOMGY010000000
VERSION     DAOMGY010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39424_length_2777_cov_2.917340"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PLR57_03210"
     CDS             <1..1185
                     /locus_tag="PLR57_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HQQ40494.1"
                     /translation="ITGADMKASSNLNEIIQNALDGGANQVYYTTISIDETALTQRLE
                     DINASLNSAPTEPSFTVEISESGKPSFNYVDGSPGYGLDVASTMQMVKDAIAAGQLQT
                     TIQPIVTAVQPTKTIADLQAHTSLIGEYTTTYDYKGTAEDTEEQRAIIPNRAFNIEKA
                     AGIINGQVIEPGERWSFNEVVGDRTEKNGWLEANGIYGGDTLKLQAGGGVCQASTTLY
                     NALLQSYPYYADTFDRSHHSFPSNYVDKGLDATVDTGHIDFAFTNNSDYPIYLFAYYS
                     KNKMASSRKRDLHVLVYGEALPEGTEYKTRPQLIETIPAGDDIIVNSSAMYVTDDPVT
                     LADRRDGYVVDVYVDRYLNGQLQESIFLYTDRYPGNPAKIKVGTKATPEPTVTPDPTI
                     EG"
     gene            complement(1244..1687)
                     /locus_tag="PLR57_03215"
     CDS             complement(1244..1687)
                     /locus_tag="PLR57_03215"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQQ40495.1"
                     /translation="MEKSKHKLLSVREDSRLLTQALRYGAAAFLGFAADYLTLLLLKE
                     TFGLHYLFAVPIAFIVGIAVNYLVGVLFVFRRGNWSMRMELSLFLGISLLALAVTELS
                     MFLLTDLVRLDYRVSRVLSGVVTYLFNFFSRRWILYRHHTHSEVK"
     gene            complement(1794..>2777)
                     /locus_tag="PLR57_03220"
     CDS             complement(1794..>2777)
                     /locus_tag="PLR57_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQQ40496.1"
                     /translation="SGIRAFVPASHVSVKYIENLSEFIGKPMKLKVLEVDRQRKRIVA
                     SHKQVLLAEAAAEKREKWEALVVGSKVNGIVRRIADFGAFVDIGGIDGLVHVTDAAWG
                     RVKHPSDVFTIGQEIEVLILNVDVEKERVSLGYKQLQPKPWMLAGEKYPIGSIVEGKV
                     VRIVPFGAFVALESTIDGLIHISQVSVHRIAKVEDELKVGDVVRCKVLEVNPEAKRIS
                     LSRKEALLEEDPELAERLKAEKAERDRQYAERQEQRTQQREVQTQQRATRDAAPRTER
                     PSNGDQDRRDASRSTDRRRRNNEDGDYELPPVEEAKTSLASLFEGLNVDDK"
BASE COUNT          666 a          757 c          731 g          623 t
ORIGIN      
        1 attaccggcg cggatatgaa agcaagttcc aatttaaacg aaatcatcca gaacgcgctt
       61 gacggcggag cgaatcaggt ttattacacg acgatctcta tcgacgaaac agcgctcacg
      121 cagcgtcttg aggatatcaa cgcatccctg aactccgcac ccacggaacc ttcgtttacc
      181 gtcgagattt ccgagagcgg aaagccttcg ttcaactatg tcgacggctc gcccggatac
      241 ggtctcgacg ttgcttccac catgcagatg gtcaaggacg cgattgccgc aggccaactg
      301 cagacgacga ttcagcccat agttacagct gttcagccta caaaaacgat agccgatctt
      361 caggcgcata cttctttgat cggtgaatat acgactactt acgactacaa aggcaccgca
      421 gaggatacgg aagaacagcg cgccatcatc ccgaaccgcg cgtttaatat tgaaaaagcc
      481 gccggaatta tcaacggtca agtgattgaa ccgggtgagc gctggagctt caatgaagtc
      541 gtgggtgatc gtactgaaaa gaacggttgg ctggaagcca acggtatcta cggcggtgat
      601 acattaaaac ttcaggctgg cggtggtgtt tgtcaggcga gtacgactct ttataatgct
      661 ctgctgcagt cgtatcctta ctatgcagat acattcgacc gctcacacca ttccttccct
      721 tcgaattatg tggataaggg actggatgct accgtcgata caggccatat agattttgcc
      781 tttacaaata attcggacta tccaatctat ctcttcgcct attattcgaa gaataaaatg
      841 gcgtccagcc gtaaacgcga tctgcatgtg cttgtttacg gtgaagcgtt gccagagggt
      901 acggaataca aaacccgtcc gcagttaata gaaacaattc cggcgggtga tgatatcatc
      961 gtaaactcca gtgctatgta tgttaccgat gacccagtga cgttagcaga tcgccgtgat
     1021 gggtatgtgg ttgacgtcta cgttgaccgc tacctcaacg gccagctgca ggaatctatc
     1081 ttcctctata ccgaccgtta tccgggcaac ccggcaaaga ttaaggttgg gacgaaagcg
     1141 acgccggagc cgacagttac tcctgatcca acaatcgaag gataattaac aattaccaat
     1201 aataaagcgt gggcgaaaaa ttcgctcgcg ctttttcttt cgtttatttc acttcactat
     1261 gcgtatgatg ccgatagaga atccaccgcc gggaaaagaa gttgaacagg tacgtaacga
     1321 cgcctgagag cacgcgtgaa acgcggtaat ccagccgaac cagatcggtc agcagaaaca
     1381 ttgaaagctc ggtgaccgca agcgcaagaa gcgagatgcc aagaaacagc gacagctcca
     1441 tccgcatgga ccagttgccg cgccgaaaga cgaacagcac gccgaccaga tagttgaccg
     1501 caatgccaac gataaacgca atcggtacgg cgaacaggta gtgcagcccg aacgtttctt
     1561 taagcagcaa gagcgtcagg tagtccgcgg caaagcccag aaaagccgcc gctccataac
     1621 gaagcgcctg cgtcagcagg cgcgaatctt cccgcacgga caagagtttg tgtttgcttt
     1681 tttccataag cgtagtatag catacccgcc tgcctgcgaa aatccccttt ttgcagttga
     1741 gtataataca taaaaagagc cgccctgttg ggcggctctt acttaacgtt tgcttatttg
     1801 tcgtcaacgt tgaggccttc gaagagagag gccagcgagg tcttcgcttc ttcgaccggc
     1861 ggcagttcat agtcgccatc ctcgttgttg cggcggcggc gatccgtgct gcgggacgca
     1921 tcacggcgat cctggtctcc gttggaggga cgctctgtgc ggggcgctgc atcgcgggtt
     1981 gcgcgctgct gggtctgcac ttcgcgctgc tgcgttctct gctcctgacg ctcggcgtac
     2041 tgacggtcgc gctcggcttt ttcagccttg agccgttcag ccagttccgg atcctcttcc
     2101 agcagagctt ccttgcggct caggctgatg cgcttggctt cggggttgac ctcgagaacc
     2161 ttgcagcgaa ccacgtcgcc aaccttgagc tcgtcttcga ccttcgcaat gcgatggacg
     2221 gaaacctgcg aaatatggat caaaccgtcg atggtgcttt ccagcgctac gaacgcgccg
     2281 aagggcacga tgcggacaac tttgccttcc acaatggagc cgatggggta cttctcgccc
     2341 gcgagcatcc agggcttggg ctgcagttgc ttgtagccaa gagaaacgcg ctctttttca
     2401 acatccacgt tgagaatgag cacttcgatt tcctggccga tggtgaatac gtcgctcggg
     2461 tgctttacgc gtccccaagc tgcgtcggtc acatgcacga ggccgtcgat gccgccgatg
     2521 tccacgaacg cgccgaagtc ggcgattctg cggacgatgc cgtttacctt gctgccaacg
     2581 acgagagctt cccacttttc gcgcttctcg gcagccgctt ccgcaagaag aacctgctta
     2641 tgagaagcga cgatgcgctt gcgctgacga tctacttcca gaaccttgag cttcatgggc
     2701 ttgccgatga actcgctgag gttctcaata tacttgacgg aaacatggga cgcgggaaca
     2761 aacgcgcgaa tgccgct
//
LOCUS       DAOMGY010000153         2777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_39426_length_2777_cov_2.800514, whole genome shotgun sequence.
ACCESSION   DAOMGY010000153 DAOMGY010000000
VERSION     DAOMGY010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39426_length_2777_cov_2.800514"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PLR57_03225"
     CDS             complement(<1..1173)
                     /locus_tag="PLR57_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQQ40497.1"
                     /translation="MKSTWKKLLALACIACMVMAQLAACAPTAAPAEETAQATTEPAA
                     EATAAEPAAEDVYAISGTVSIAYPEGEVAEIQPVLDAFRAQYPNVTVEEVPFAGSTGG
                     AFNEFLTQRAAANDMPDVMWLDWNDFAPEVASGFVMPLNDLMAGDPDAEYVPAGMTDP
                     YTYNGKLYALPCQMNAMGITINLDMLEALNIEKPSYDWTFAEFEDLLKKAISADSVGA
                     ATLEDLDQVYSAQAAGYMYPAYDAANQKFDFTNMWVPAMNKLAELRAIPGLEAWSMRF
                     PKNEDGTTSMDSEYVAKFGEAGKDDNHYTFKNGMALLCTNATWNDNWMRSECKVTWDY
                     WPYPRMDESTPVYTPIHVDCAYLTSTCAEPEVAFQLLKWLTYGVEGNLQRLDIFAAR"
     gene            complement(1323..>2777)
                     /locus_tag="PLR57_03230"
     CDS             complement(1323..>2777)
                     /locus_tag="PLR57_03230"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HQQ40498.1"
                     /translation="EDPAAQAACLLEENEADYDALLQKNAAVWEAIWRTADIRLDASD
                     ELQGAVRYNIYQLIASAPHGDEHASIGARGLMHGRYKGCYFWDTEVFMLPFFRDTLPD
                     TAKSLLMYRYNTLNDAIESARRFSVKGARYSWMSSDTGFEQCETWDTGCCEIHITADI
                     AYAVGKYALLSGDKAFMDEFGARILVETARYWFDRFSYSELEDRYHLLFVKGPDEYCG
                     VTSDNLYTVRLSKHNIALALQTLERLKKEQPGRYETLVQATALLPEETVNWQDLLEKT
                     VERFDEGRNLWVQDATFEQLEPLDIPGVRDTCKPLYYTLNFDRLQRYRVLKQPDVLML
                     MALLPEEFSQAEIESAWDYYEPITLHDSTLSFGMHALVAARLNKQAEAQGYFEKALYM
                     DLRDVMQNTAREGIHTAALGAAWQALVYGYCGLSADPGAPRCTPRLPAFIRSAAFTVF
                     RDGIWYDVTVNREGSCTVSKIQNPNPNSVFLRRV"
BASE COUNT          499 a          769 c          864 g          645 t
ORIGIN      
        1 gcgggcggca aagatatcca ggcgctggag gttgccttct acgccgtagg tcagccactt
       61 gagcagctgg aaggcgactt cgggttcggc gcaggtgctg gtcaggtacg cgcagtccac
      121 atggattggc gtgtagacgg gggtgctttc atccatacgc gggtagggcc agtagtccca
      181 ggtgactttg cactcggaac gcatccagtt gtcgttccag gtggcgttgg tgcagagcag
      241 agccatgccg tttttgaagg tgtagtggtt gtcgtccttg ccggcttcgc cgaacttggc
      301 aacatattcg ctgtccatgg aggtcgtgcc gtcttcgttc ttcgggaagc gcatgctcca
      361 ggcttcgagg ccggggatgg cgcggagttc ggcgagcttg ttcatggccg gaacccacat
      421 gttggtgaag tcgaacttct ggttcgcggc gtcgtatgcg ggatacatat atcctgcggc
      481 ctgcgcagag tagacctgat cgaggtcttc gagcgtggct gcgccgacgg aatcggcgga
      541 gatggccttc ttgagcaggt cttcgaactc ggcgaacgtc cagtcatagc tgggcttttc
      601 gatgttgagc gcttcgagca tgtcgaggtt gatcgtaatg cccatggcgt tcatctggca
      661 gggcagagcg tagagcttgc cgttgtaggt gtaaggatcg gtcatgccgg cgggaacgta
      721 ttccgcatcc gggtcgcccg ccatcaggtc gttcaggggc ataacgaacc cgctggcaac
      781 ttcgggagca aagtcgttcc aatccagcca catgacgtcg ggcatgtcat tggcggcggc
      841 acgctgggtc aggaattcgt tgaacgcgcc gcccgtgctt ccggcaaacg gaacttcctc
      901 gacggtcacg ttgggatact gcgcgcggaa cgcgtcgaga acgggctgaa tctcggcgac
      961 ttcgccttcc ggatacgcaa tggagacggt gccggagatg gcgtaaacgt cctcggcggc
     1021 gggttcggct gccgtggctt ccgcggcggg ttcggtggtt gcctgtgccg tttcctcggc
     1081 gggagcggct gtcggcgcgc atgccgcaag ctgagccatc accatgcagg cgatgcaagc
     1141 cagcgcaagc agtttcttcc atgtgctttt cattgtttct ctcctttgac ttgttttttc
     1201 ttttcaagtc gttccggcag cagatttgac agtcagtttc cattttatcg tatccgcggc
     1261 aggaacgctt agaaaattta taagcgtttt gaaagctttt attgaagctt ttattgaagc
     1321 ggttaaactc tgcgcaaaaa aacgctgttc ggattcggat tttgaatttt tgatacggtg
     1381 cagcttccct cgcggttcac ggttacgtcg taccagattc catcccggaa cacggtgaac
     1441 gcggcggaac ggatgaacgc cggcaggcgg ggcgtgcagc gcggtgctcc cggatcggcg
     1501 gaaagcccgc agtaaccgta gacgagcgcc tgccaggctg cgcccagcgc cgcggtatga
     1561 atgccttcgc gggcggtgtt ttgcatgacg tcccgcagat ccatatacag cgctttttca
     1621 aagtacccct gcgcctcggc ctgtttgttg agccttgccg caaccagcgc gtgcatgccg
     1681 aagctgagcg tggagtcgtg cagagtaatg ggctcgtagt agtcccacgc ggactcgatt
     1741 tccgcctgcg aaaactcttc cggcaagagc gccatgagca tgagcacatc cggctgcttg
     1801 agtacgcggt aacgctgcag gcggtcgaag ttgagcgtgt agtacagggg cttgcaggta
     1861 tcccttacgc cagggatatc gagcggttcc agctgttcaa acgttgcgtc ctgcacccag
     1921 aggttgcggc cttcatcgaa gcgctccacg gttttttcga gcagatcctg ccagtttaca
     1981 gtctcttccg gaaggagcgc ggtcgcctgc accagcgttt cataacgtcc gggctgttcc
     2041 tttttgagcc gttcaagcgt ttggagcgcg agggcgatgt tgtgcttgga tagacgcacg
     2101 gtgtagaggt tgtcgctcgt gacgccgcag tattcgtccg gtcccttcac aaacagcagg
     2161 tggtagcggt cttcaagttc gctgtagctg aagcggtcga accagtaacg cgcggtttcc
     2221 accaggattc tcgcgccaaa ctcgtccata aatgctttgt ccccgctcaa gagcgcgtat
     2281 ttgccgacgg cgtaagcgat atccgccgta atatggattt cgcagcagcc cgtgtcccag
     2341 gtttcgcact gttcaaaccc ggtgtcggac gacatccagg aataccgcgc gcccttgacc
     2401 gagaagcgcc gcgcgctctc tatagcgtcg ttcagcgtgt tgtagcggta catcaaaagg
     2461 cttttcgccg tatcgggcag cgtgtcgcgg aagaacggca gcataaagac ttccgtatcc
     2521 cagaaataac agcctttgta tctgccgtgc atcaggccgc gcgcgccgat gctcgcgtgt
     2581 tcgtcgccgt gcggcgcgct ggcaatgagc tgatagatgt tgtaccgaac cgcgccctgc
     2641 agttcgtccg aagcgtccag ccggatatcc gcggtacgcc agatggcttc ccagacggcc
     2701 gcgtttttct gcagcagcgc gtcgtaatcc gcttcgtttt cttccagcag gcaggctgcc
     2761 tgcgccgcgg ggtcttc
//
LOCUS       DAOMGY010000154         2764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_39697_length_2764_cov_3.540052, whole genome shotgun sequence.
ACCESSION   DAOMGY010000154 DAOMGY010000000
VERSION     DAOMGY010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39697_length_2764_cov_3.540052"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..759)
                     /locus_tag="PLR57_03235"
     CDS             complement(76..759)
                     /locus_tag="PLR57_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HQQ40499.1"
                     /translation="MLGTIVNAAAILVGGSLGLLLKKGIPERVSSGIVQGLALIVLYI
                     GISGMLKGQNALIATISIVVGGVIGILLDLDGRFKRLVGKLETRFVKTETGGSTFGEG
                     FMTATLLFCVGAMAVVGSLNSGLTGNHEVLYTKSLLDGVSAMIFASTLGAGVLLSAVA
                     VFVYQGAITLAAQFLAPVLSDATVAEMTCVGSLLIIAIGLNMLKLTKIKVMDFVLVIF
                     VPIGLCMFM"
     gene            complement(899..>2764)
                     /locus_tag="PLR57_03240"
     CDS             complement(899..>2764)
                     /locus_tag="PLR57_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQQ40500.1"
                     /translation="AFNRSVNWIPETIKEGRMGNFLENVIDWSVSRERYWGTPLPVWM
                     CECGHVHVVGSRAELLELGNDVPADIELHKPYIDEITFPCPKCGKPMKREPVVIDCWF
                     DSGAMPFAQWHYPFEHKEEFERRYPAQFISEAIDQTRGWFYSLLAISTCLFDEAAFQN
                     CIVMGHVQDMEGRKMSKHLHNVVDPWTVLNAQGADAVRWYFYTGSMPWLPSRFSAESV
                     SEAQRKFIGTFWNTYAFYVLYAEIDKFDPTQYSLKDCALSEMDRWVLSRLNTLVKTVD
                     GYMDKLKITEAGRALQAFSEELSNWYVRRCRDRYWGSEMTEDKKAAYMTLYTALHTLT
                     LLTAPFLPFMTEEIYQNIVRSVDASAPESVHFCDYPAADEAYIDTRLEADMARVLDIV
                     TLGRAARCDGAVKTRQPLALMYIQGEPLSERYVAIVTEELNVKKAEFLADASGFLSYV
                     VKPQLKTLGPRYGKILPKINAYLAQPGIGNAVVRAHSSGKDYETELEGVLVVLSPEDV
                     LVSTGKTDGFVAATDNDTIVVLDTKLTPELIDEGFVRELISKVQTMRKEAGFEVSDHI
                     TLSYDGSPKIEEIFARFGSDILAETLGDRLEKGAKGYTKDWDVNKEPVTLGVEKL"
BASE COUNT          572 a          780 c          813 g          599 t
ORIGIN      
        1 acgaatgagc tcctaggttg gtaatagagt cgtccctttg cttcttttct tgcaaaaaaa
       61 gaagaagaga accctttaca tgaacatgca gagtccgatg ggaacgaaga tgacaaggac
      121 aaagtccatc accttgatct ttgtgagttt gagcatgttc agcccgatgg cgatgatcag
      181 aagcgaccct acgcaggtca tttcggcaac cgtcgcatcg ctgagtacgg gggctaaaaa
      241 ctgcgcggca agcgtaatgg cgccctgata gacaaacacc gccacagcgg agagcagaac
      301 acccgcgccg agcgtgctgg caaagatcat tgcgcttacg ccgtcgagca ggctcttggt
      361 atagagcacc tcgtggttgc cggtgaggcc gctgttgagc gaaccaacga ccgccatggc
      421 gccgacgcag aagagcagcg tcgccgtcat gaacccttcg ccgaaggtgg aaccgccggt
      481 ttcggttttc acaaaccgcg tttcgagctt gccgacgagc cgcttgaacc gtccgtcgag
      541 atccagcagt atgccgatca cgccgccgac aacgatggag atggtcgcaa tcagcgcgtt
      601 ctggcctttg agcatgccgc tgataccgat gtagaggacg atcagcgcaa gtccctgcac
      661 gatgccgctg gagacgcgtt ccggtatgcc ttttttgagc agtaagccga gcgaccctcc
      721 aacgaggatc gctgcggcgt tgacgatggt tccaagcatg aaggcctttc ttcttacttt
      781 ctttgaagaa agtaagctaa agaatatttt cctttctttg aaaagaaagg aaacgaaaga
      841 aattttctgg ttatcgagag aatttctttg ctaagctttc ttttaaaaga aagcgcaatt
      901 acagtttctc tacgcccagc gtaacgggct ctttgttcac gtcccagtcc ttcgtgtagc
      961 ccttcgcacc cttttcgagc cggtcgccca gcgtttcggc gaggatgtcg ctgccgaagc
     1021 gggcgaagat ctcctcgatc ttcgggctgc cgtcgtagga aagcgtgatg tggtcgctga
     1081 cctcgaagcc cgcttccttg cgcatggtct gcaccttgct gatgagctcg cgcacgaaac
     1141 cttcgtcgat gagttccggc gtgagtttgg tatccagcac gacgatggta tcgttgtcgg
     1201 tcgcggcgac aaatccgtcg gtcttgccgg tggaaacgag cacgtcctcc ggcgagagca
     1261 cgaccagaac gccttcaagc tccgtttcgt agtctttgcc ggagctgtgc gctctgacga
     1321 ctgcgttgcc gatgccgggc tgggcaaggt acgcgttgat ttttggcaga atcttgccgt
     1381 agcgcggccc gagcgtcttg agctgcggct tgacgacgta gctcaaaaac ccggaagcgt
     1441 ccgcgaggaa ctccgccttc ttgacgttca gctcttcggt cacgatggcg acgtaccgct
     1501 cggaaagcgg ttcgccctga atgtacatca gcgccagcgg ctggcgggtc ttgacggctc
     1561 cgtcgcagcg cgcggcacgg ccgagggtga cgatatcgag tacgcgcgcc atatccgctt
     1621 ccagccgcgt atcgatatac gcttcgtctg ccgcgggata gtcgcagaaa tgcacgcttt
     1681 cgggcgcgga ggcgtccacg ctgcggacga tgttctggta gatttcctcc gtcataaacg
     1741 gcaaaaacgg cgcggtgagc agcgttaaag tatgcagcgc ggtgtagagc gtcatatacg
     1801 ctgccttctt gtcctcggtc atctcactgc cccagtagcg gtcgcggcag cggcggacat
     1861 accagttgga aagttcttcc gaaaacgcct gcagcgcgcg cccggcttcc gtgatcttga
     1921 gcttgtccat atagccgtcc acggttttaa cgagcgtgtt gaggcgcgag agcacccagc
     1981 ggtccatctc ggaaagcgcg cagtccttga gcgaatactg cgtgggatcg aacttgtcga
     2041 tctccgcgta gagcacgtag aacgcatagg tgttccagaa ggtgccgatg aactttctct
     2101 gcgcttcgct gacggattcc gccgagaagc ggctgggcag ccaaggcatg gagccggtat
     2161 agaagtacca gcgcaccgcg tccgctccct gcgcgttgag taccgtccag ggatcgacca
     2221 cgttgtggag gtgcttgctc atcttgcggc cttccatatc ctgcacgtgc cccatgacga
     2281 tgcagttctg gaacgcggct tcgtcgaaca ggcaggtgga aatggcaagc agggaataga
     2341 accatccgcg cgtctggtcg atggcttcgc tgatgaactg cgcgggatag cggcgttcaa
     2401 attcttcctt atgctcaaat ggatagtgcc actgcgcaaa gggcatggcg ccgctgtcga
     2461 accagcagtc gatgacgacc ggctcgcgct tcatcggctt tccgcacttt ggacagggga
     2521 aggtgatttc gtcgatgtac ggcttatgca gctcgatatc cgcgggaaca tcgttgccca
     2581 gttcgagaag ctccgcgcgg gaaccgacaa cgtgcacatg cccgcactcg cacatccaga
     2641 ccggcagcgg cgtgccccag tagcgttcac gggagacgct ccagtcgatg acgttttcga
     2701 ggaagttacc catgcggcct tccttgatgg tctccggaat ccagttgacg ctgcggttga
     2761 acgc
//
LOCUS       DAOMGY010000155         2752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_39967_length_2752_cov_3.604375, whole genome shotgun sequence.
ACCESSION   DAOMGY010000155 DAOMGY010000000
VERSION     DAOMGY010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39967_length_2752_cov_3.604375"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..978)
                     /gene="heR"
                     /locus_tag="PLR57_03245"
     CDS             complement(199..978)
                     /gene="heR"
                     /locus_tag="PLR57_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HQQ40501.1"
                     /translation="MELKYQKLRRFNLIMGAVHLVQAVIMLFLATSVIQKIAEFQPTI
                     TQNYLTFNVATKSLESAGKALFQLPFGILVASFLFISAVAHGIIYLSGEKYIDGIRNG
                     INKYRWFEYALSSSIMIVLISTLFGIYDIASLILIFVVNASMNLFGLVMEQLNSGAEK
                     SKVKWGPFVWGSIAGIAPWVAILLYMFGTGNFDMVPWFVWAIVGTYFVAFNTFPINMV
                     LQYKRVGKWKDYAYGERVYIVLSLVAKSFLAWLVLFGAMQP"
     gene            complement(1118..1891)
                     /locus_tag="PLR57_03250"
     CDS             complement(1118..1891)
                     /locus_tag="PLR57_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ40502.1"
                     /translation="MLELNDIQTTFNAGSPDEMRLFTGFSLAVQEGQFVSVIGSNGSG
                     KTTLLNLVCGTMRPDAGKIVFDGRDITGVSAVKRASWIGRVFQDPKAGTCSHLTILEN
                     LALADNKGSAYGLGRGVLKSRREHYRALLSQCGMGLENRLDTLVGTLSGGQRQALALV
                     IATMSDIRLLILDEHTAALDPKSSETIMELTNELVKKRNLTTVMVTHNLRYAVAYGDR
                     LLMMHRGEAVIDRSGEQKQALAVEDLLDTFTSISVELGN"
     gene            complement(1884..>2752)
                     /locus_tag="PLR57_03255"
     CDS             complement(1884..>2752)
                     /locus_tag="PLR57_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40503.1"
                     /translation="FGVYLTYSILDFPDLSVDGTMPLGAILSGVLILHGVNPWLCLLA
                     AFLAGAGFGSITGILHVKLKIRPLLSGILMFTALLSVNLVILFLFNGGNSVASFFRKP
                     TIFSAFPASLIPETVGGFYIHQLVVSLVLVIVCKVLLDAFLRTKCGLLLRATGDNAGF
                     VTMLSRDPGQSKITGLAIGNGFAALSGAVIAQAKGSSDQQMGTGMVVLGLASVIIGLS
                     LFKGFRRIRPTTAVVFGSVAYKACLSVALALGLPTEYLKLLMAALFVLALIAGEKPMF
                     QSLLKRRQSHAA"
BASE COUNT          632 a          778 c          784 g          558 t
ORIGIN      
        1 ggtgccgcgc gtgttcctca aagccgcgct gcagggctgc gtggactggg caaagcagaa
       61 cggcgtgagc gtgctggacg tagagcatat ggcaattatc cgcgacaaac gcggcgcgga
      121 aaagaagaag taacgcattt taagcaaaca aaaacgccag gtgaccggcg tttttttgat
      181 atccagcttt ttgcggcgtt acggctgcat cgctccaaag agcacgagcc aggccagaaa
      241 cgattttgcc acgaggctca gcacgatata cacgcgctcg ccataagcgt agtccttcca
      301 tttgccgacg cgcttatact gcagcaccat gttgatgggg aacgtgttga acgctacgaa
      361 gtacgtgccc acaatcgccc agacgaacca gggcaccatg tcgaaattgc ccgtgccgaa
      421 catgtacagg aggatcgcca cccatggcgc aatccccgcg atggaacccc agacgaacgg
      481 gccccatttt actttggact tttccgctcc ggagttgagc tgctccatca cgagcccgaa
      541 gaggttcatg gacgcgttga ccacaaagat cagaatcagc gacgcgatgt cgtatatacc
      601 gaacagggtg ctgatgagaa cgatcatgat cgacgagctc agagcgtact cgaaccagcg
      661 gtacttgttg atgccgtttc gaatgccgtc gatgtacttt tctccgctga gatagatgat
      721 tccgtgcgca accgccgaga taaagagaaa cgaggcaacg aggattccga acggcagttg
      781 gaacagcgct ttgcccgcgg attcgagcga ttttgttgcg acgttgaagg tcagatagtt
      841 ctgcgtgatc gtcggctgaa attccgcgat cttctgtatg acgctggttg ctaaaaagag
      901 catgatcacg gcctgaacca gatggacggc acccatgatc aaattaaacc ggcgaagttt
      961 ttgatatttc aattccattt gtatgccctc cctttctggc tctatcatac tatatttaca
     1021 gcggttttcg gtgatttcat cacaaatgtt atatgctaac acaaaacacc ggcgttattg
     1081 ccggtgcttt tgtgtttttc acctgtttgt attcttctta attgccgagt tcgacgctga
     1141 tcgacgtgaa cgtgtccagc aggtcttcca cggcgagcgc ctgcttctgt tcgccggaac
     1201 ggtcgatgac tgcttcgcct cgatgcatca tcaaaaggcg gtcgccgtag gctacggcgt
     1261 agcgcagatt gtgggtgacc atcacggtag tcaggttccg cttttttacg agctcgttgg
     1321 tcagttccat gatggtttcg gaggatttcg ggtcgagcgc cgcggtatgc tcgtccagaa
     1381 tcaagagacg gatatcgctc atggtcgcaa tcacgagcgc cagcgcttga cgctgcccgc
     1441 cggaaagcgt gccgaccagc gtgtcgagcc ggttctccag ccccatgccg cactggctca
     1501 agagcgcgcg gtaatgctcc cgtctgcttt tcaaaacgcc gcgccccaga ccgtaggcac
     1561 tgcctttatt atccgccagc gcaaggtttt ccaaaatcgt cagatgcgag caggtgcctg
     1621 ccttggggtc ctgaaacaca cgtccgatcc aggaagcgcg cttgacggcg gaaacgccag
     1681 tgatgtcccg cccgtcgaac acaattttgc ccgcatcggg gcgcatggtg ccgcagacga
     1741 ggttgagcag cgtcgtcttg ccgctgccgt tggagccgat cacactgacg aactgcccct
     1801 cctgaacggc gagcgaaaag cccgtaaaca ggcgcatttc atccggagat ccggcgttga
     1861 acgtggtttg aatatcgtta agttcaagca gcatggctct gcctcctttt gagcaacgac
     1921 tggaacatcg gcttttcgcc cgcaatcagc gcgagaacga acagcgccgc catgagcagc
     1981 ttgagatatt ccgtcggcag gccgagcgcc agcgcaacgg agaggcacgc tttgtacgca
     2041 acgctgccga acaccaccgc cgtggtcggg cggatacgcc gaaaaccttt gaacaggctc
     2101 aggccgatga tgaccgaagc gagccccagc acgaccatac ccgttcccat ctgctggtcg
     2161 gaggaaccct ttgcctgcgc gatgaccgcg ccggaaagag ccgcaaaccc gttgccgatg
     2221 gcaaggccgg tgattttgct ctgtcctgga tcgcgagaga gcatggtcac aaaccccgcg
     2281 ttgtcgccag ttgcgcggag cagcagcccg cacttggtgc gtaaaaacgc gtccagaagc
     2341 accttgcaga caatcacgag cacaaggctc acaacgagct ggtggatgta gaagccgccg
     2401 acggtttccg gtatcagcga cgcgggaaac gccgagaaga tcgtgggctt gcggaagaac
     2461 gaagccacgc tgtttccgcc gttgaacaga aacaggatca caaggtttac cgatagcagc
     2521 gccgtgaaca tcagaatccc gctgagcaga gggcgaatct tcagcttgac gtgcagtatt
     2581 cccgtgatgc tgccaaagcc cgcgcccgcg agaaacgccg cgagcaggca gagccagggg
     2641 ttcacgccgt ggagaatcag cacgcccgaa aggatcgccc ccagcggcat ggtgccgtcc
     2701 acggacaaat cggggaagtc gaggatgctg taggtcaggt aaacgccgaa cg
//
LOCUS       DAOMGY010000156         2750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_40015_length_2750_cov_3.559555, whole genome shotgun sequence.
ACCESSION   DAOMGY010000156 DAOMGY010000000
VERSION     DAOMGY010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40015_length_2750_cov_3.559555"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLR57_03260"
     CDS             complement(<1..531)
                     /locus_tag="PLR57_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQQ40504.1"
                     /translation="MFIKKGTSKSYSAAFFDRDGTLLHGDPAARDARRAMIERWSGKP
                     LDAPDDLFFQVLGDRRLLTVENEIAFWKQYFTALLRSQGIADRIEERANELFDAYWLK
                     GIVVYPEVVPVLDWFFERGFRMGVISDTFPSLPLTIEAAGLGKYFSCYVCSDQVGAFK
                     PDPRMYAAIAEKLNAEP"
     gene            complement(623..1261)
                     /locus_tag="PLR57_03265"
     CDS             complement(623..1261)
                     /locus_tag="PLR57_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40505.1"
                     /translation="MEEDFDLEKCLIALRKKSSVFHSEADFQFALAWETQLAYPNAVV
                     HLEYCPKAMPNMHIDIVVFYGEKFYPIELKYKTANLDVDSGSEAFHLKNHGAQDIGKY
                     DCMLDIQRIESMRGVIPNFEKGYAVWLTNDSSYWKAPSKASAFYREFSIHEGSVKSGV
                     MCWDEETGEGTTKGRTEPIVLRGTYSINWKPYSTIKTDRNGAFYYALVEVNQ"
     gene            complement(1288..2592)
                     /locus_tag="PLR57_03270"
     CDS             complement(1288..2592)
                     /locus_tag="PLR57_03270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HQQ40506.1"
                     /translation="MRRPLPKEYIERMRDLLGEDGLSAYLEAMLQPQKRTLRVNTLKT
                     SAEEFRAKADFPLEPVELVADSFFFDDDVQIGKHPLHAAGLCYVQEPSAQVPASLLDV
                     RPGMTVLDLCAAPGGKATQLAALMQNRGLLVVNEVVRSRTDTLLGNIERLGVSNALVV
                     SARPDALEDPLCGLFDRILVDAPCSGEGMFRKDETAVGVWSPEHVASCANRQGAILHS
                     AVEMLKPGGRLIYSTCTFSKEENEGVIKEFLDMHSGFKLLETRRLYPHTSVGEGQFMA
                     VLERTDDDAPPAEQLTETPLERLPAWEAFWRDTLLGEPPGVLTVHDGRVLLPPEKLPA
                     SLIRLHALRAGILAGEMDYRAGRFTPAHALAMAFPAAAFQRTVPLEGQELTRFFSGET
                     VPCDPALSGWCAVTALGHPIGWGKAVQGVLKNHIPKGLRFYS"
BASE COUNT          579 a          824 c          739 g          608 t
ORIGIN      
        1 gggctccgcg tttaattttt ccgcgatcgc agcgtacatg cgcgggtcgg gcttgaacgc
       61 gcccacctga tcggagcaga catagcagga gaagtacttg ccgagtccag ccgcctcgat
      121 ggtcagcggg agcgaaggga acgtgtcgct gataaccccc atgcggaagc cgcgctcgaa
      181 aaaccagtcc agcaccggca cgacctccgg gtacacgaca atccctttga gccagtacgc
      241 gtcaaagagt tcgttggcgc gttcttctat gcggtcggca atcccctgcg agcgcagcag
      301 cgcggtaaaa tactgcttcc aaaacgcaat ctcgttctcc accgtcagca gacggcggtc
      361 gcccagcacc tgaaaaaaca ggtcgtccgg cgcgtccagc ggtttgcccg accagcgttc
      421 gatcatcgct ctgcgcgcat cccgcgcggc ggggtcgccg tgcagcagcg tgccgtcgcg
      481 gtcgaaaaac gccgcggagt aggacttgga tgtacctttt ttaatgaaca ttcttatatc
      541 ccgccttaat catgtaagga tatttgtttc tatcgttcta gtcagagatt cccgcttctg
      601 gcatttcatt cgtcttttcc ggttattgat ttacctcgac gagcgcataa taaaacgccc
      661 cgttacgatc tgtttttatt gtggagtagg gtttccagtt gatcgagtaa gttccgcgta
      721 aaactatcgg ttccgtacga ccttttgtcg ttccctcgcc cgtttcttca tcccagcaca
      781 taacgccgct ctttacactg ccttcgtgaa tggaaaactc tctgtaaaaa gcactggctt
      841 tgctaggagc tttccaatag gacgaatcgt tcgtcagcca aacagcatac cctttttcaa
      901 aattcggaat gacaccccgc attgattcaa tgcgctgtat atcaagcatg cagtcatatt
      961 taccaatatc ctgcgctcca tgattcttca aatggaatgc ttcggaaccc gaatctacgt
     1021 ccagattcgc agttttgtat ttcaactcga tcggataaaa cttttcgccg taaaacacga
     1081 caatgtctat gtgcatgttt ggcattgcct tgggacagta ttccaaatgc acaaccgcat
     1141 tcggataagc aagctgtgtt tcccatgcca gagcgaactg aaagtccgct tccgaatgga
     1201 aaactgagct cttttttcta agtgcaataa gacacttctc cagatcgaag tcttcttcca
     1261 taatgcatcc ttatttaata ctaatattta cgaataaaac cttaacccct tcgggatatg
     1321 attcttgagc acgccctgca ctgccttgcc ccagccgatg ggatgcccca gcgcggtgac
     1381 ggcgcaccag ccggaaagcg cggggtcgca cggcactgtt tcgccggaga agaagcgcgt
     1441 gagttcctgc ccctccagcg gcaccgtccg ctgaaatgcg gcggcgggaa acgccattgc
     1501 cagcgcgtgc gcgggggtaa agcgcccggc gcggtagtcc atctcgcccg cgagaatgcc
     1561 cgcgcgcaga gcgtgcagcc gtatcaggct ggcggggagc ttttccggcg gcaggagcac
     1621 gcgcccgtcg tgcacggtga gcacgcccgg cggctcgcca agaagcgtat cccgccagaa
     1681 ggcttcccat gcgggcaagc gctccagcgg cgtttctgtg agctgctcgg cgggcggcgc
     1741 gtcgtcgtcc gtccgctcca gcacggccat gaactgccct tcgccgacgg acgtatgcgg
     1801 gtagagccgc cgggtttcga gcagtttgaa tcctgaatgc atgtcaagaa attccttgat
     1861 aaccccttcg ttttcctctt tggagaacgt gcaggtggag tagatgagcc gcccgccggg
     1921 cttgagcatc tccaccgcgc tgtgcagaat cgccccctgc cggtttgcgc aggaagcgac
     1981 gtgctccggc gaccagaccc cgacggcggt ttcgtccttg cggaacatcc cttcgcccga
     2041 gcaaggcgca tccaccagaa tgcggtcgaa cagcccgcag agcgggtctt ccagcgcgtc
     2101 cggccgcgcg ctgacgacaa gcgcgttgct taccccgagc cgctcaatgt tgcccagaag
     2161 cgtatcagtc cgagaacgga cgacttcgtt gaccacgagc agtccccgat tctgcatcag
     2221 cgccgcaagc tgcgtagctt ttccgccggg tgcggcacaa aggtcgagca ccgtcatgcc
     2281 ggggcgaacg tctagcaggc tcgccggaac ctgcgcggaa ggttcctgca cgtagcatag
     2341 ccccgccgcg tggagcgggt gcttgccgat ttgtacgtca tcgtcaaaaa agaaactgtc
     2401 cgcaacgagt tcaacgggtt cgagcgggaa gtctgctttc gcgcggaact cctccgcgct
     2461 cgttttgagc gtgttgaccc taagcgtgcg cttttgcggc tgcagcatcg cttcaaggta
     2521 ggcgctcaga ccatcctcgc caagcaggtc ccgcatccgc tcaatatact cctttggcag
     2581 tggccgccgc atcgcattct cctctgaaac atatttctta tattttagca taaaacacaa
     2641 ggggttctta ggggctttgc ccctaagtcg gaggtttgga ggcgaagccc caagaaaata
     2701 atagtaaaag ccacctcaca aacaacggct cccttgcggg agccgcgttg
//
LOCUS       DAOMGY010000157         2740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_40232_length_2740_cov_2.757169, whole genome shotgun sequence.
ACCESSION   DAOMGY010000157 DAOMGY010000000
VERSION     DAOMGY010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40232_length_2740_cov_2.757169"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLR57_03275"
     CDS             complement(<1..321)
                     /locus_tag="PLR57_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQQ40507.1"
                     /translation="MINACDAQVLPRKSGRILSLGELLIDLIPTETGGRIDQAGAVWK
                     TASGSAGIFACAVAALGAESAFLGKVGTDALSRMAYHTVREAGVDMSRVVVSGEGQIG
                     LAFIE"
     gene            630..1736
                     /locus_tag="PLR57_03280"
     CDS             630..1736
                     /locus_tag="PLR57_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQQ40508.1"
                     /translation="MKKVTSQDVAARAGVSQSAVSLILNESEKVLFNEETKARVIAAA
                     KELGYELPKRRKRLPASRTGILLLFTPTLNNPFYAELVQTVESYADACGYCVIVCNTL
                     RKPELERYYLETFLKMHVDGMIFTFLPSFPEIVESVADSVPSVLIGEKRIEQPICSIE
                     LSNERAGNILAEHLYALGHRKFLFVSTPFNQLTLARQQRLEGIRAFLRAQRLDESSLE
                     LLASEAQTETDALPDAMPYEYAVGRKLIGQAMERGLNATAIIGVNDMTAIGLMDEIKA
                     RGYSVPGDFSVCGFDNIFTSAISSPQLTTINHHLQVRCKAAVDQISARRRELSEATLP
                     PVINKVEYSPRLVVRASTGPAREGAPVRKRDSHA"
     gene            1729..2628
                     /locus_tag="PLR57_03285"
     CDS             1729..2628
                     /locus_tag="PLR57_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40509.1"
                     /translation="MLKNLFIPKPAPEDLPPQERPITRKALFKQTFGNYWSWFFAINL
                     VCFLFYLPASVWTEMSLSSLIAEESATFQTSAFMGAYLLGLAVCLFLTGPMLAGVTLL
                     MRSWSRGEPCMRWQTLFGGMKRNWKQALGFSLIEGLMPLAAYSTLRYYGGLGDSAGVG
                     YYVLFGFCALVILFALLMRQLVYTLTVTYELKFGQIVKNAFLLTFLELPKSLWTLLKN
                     SLPVAVFALLLWLLPAYTGLIVILGLAYYALFGVALERFVSASFANYVVEKHINSKLS
                     GARVDIGMASERVVSSESSQERE"
BASE COUNT          589 a          773 c          777 g          601 t
ORIGIN      
        1 ctcgatgaac gcaaggccga tctggccttc gcccgaaacc accacgcggc tcatatccac
       61 gcctgcttcc cggacagtat ggtaggccat gcggctcagc gcgtccgtac caaccttgcc
      121 gagaaaggcg ctttcggcgc ccagcgcggc gacggcgcaa gcaaaaatac cagcgcttcc
      181 actggccgtt ttccatacgg cgcccgcctg atcgatgcgt ccgccggttt ccgttggaat
      241 caggtcaatt aagagttcgc caagcgagag aattcgtccg ctcttgcggg gcagtacttg
      301 cgcgtcgcat gcgtttatca taatgcattc cccttgggcg cagagctgcg ccgggttttc
      361 attttgctat tcgactgcaa cgcgactgga aacggaatta ctaatactca catctaataa
      421 aatagcaaat aatagggaat aagtcaagaa gcggacggca aatccggcgg cttttttcgc
      481 gcgaattatc gcagccgcgc gtaaaaacag cgtattcagc caaaattcag gctcgcttta
      541 caaaaaagca tttgacagaa acgttgaatt aagataatat ttattaggaa ttattagcac
      601 atttcaacca tattacgggg gcataccgca tgaaaaaagt cacttcgcag gacgttgccg
      661 cgcgggcggg ggtttcgcag tccgccgtgt cgctcattct caacgaaagc gagaaggtgc
      721 tctttaacga ggagaccaag gcgcgcgtca tcgccgcggc aaaggaactg ggctacgaac
      781 tgccaaagcg cagaaagcgc ctgcctgctt cccgcaccgg catcctgctg ctgtttacgc
      841 caacgctgaa caatcccttt tacgccgagc tcgtgcagac cgtcgaaagt tatgccgacg
      901 cctgcgggta ctgcgtgatc gtctgcaaca cgctcagaaa gccagagctg gagcggtatt
      961 atctggaaac gttcctcaaa atgcacgtag acggcatgat ctttaccttt ttgccgagct
     1021 ttccggagat cgtggaaagc gttgcggaca gcgtgccttc cgtgctgatt ggcgaaaagc
     1081 gcatcgagca gcccatctgc tccatcgaac tgagcaacga gcgcgcgggc aatatcctcg
     1141 ccgagcacct ctacgcgctg gggcaccgca agttcctttt cgtctccacg ccgttcaacc
     1201 aactgaccct cgcgcgtcag cagcggctgg aaggaatccg cgcgttcctg cgtgcccagc
     1261 ggctggacga aagcagcctt gagctgctcg cttccgaagc gcaaacggag accgacgccc
     1321 tgccggacgc catgccgtat gaatacgccg tcggcagaaa gctcatcggc caggcgatgg
     1381 agcggggctt gaacgcaacc gcgatcatcg gcgtcaacga catgacggcc atcggactca
     1441 tggacgaaat caaggcgcgc ggctacagcg tgccgggaga cttttcggtc tgcgggtttg
     1501 acaacatctt tacctccgcc atctcctcgc cgcagctgac cacgattaac caccacctgc
     1561 aggtgcgctg caaagccgcc gtggatcaaa tcagcgcgcg caggcgcgag ctcagcgaag
     1621 cgacgctgcc gccggtcatc aataaagtgg agtattcgcc gcgtcttgtg gtgcgggctt
     1681 ccaccggccc cgcgcgcgaa ggcgcgccgg tgagaaagcg cgacagccat gcttaagaac
     1741 ttatttattc ccaaacccgc gccggaggat ttacccccgc aggagcggcc aataacgcgc
     1801 aaggcgctat ttaagcagac gttcggcaat tactggagtt ggttttttgc cattaatctt
     1861 gtctgcttcc tcttttatct gcccgcgagc gtctggacgg agatgtcgct gagctctttg
     1921 attgccgagg agagcgcaac gtttcaaacg agcgctttta tgggcgcgta cctgcttggg
     1981 ctcgcggtct gcctgtttct tacggggccg atgctcgcgg gggtgacgct tttaatgcgt
     2041 tcctggtcgc gcggcgaacc ctgcatgcgc tggcagacgc tttttggcgg catgaagcgc
     2101 aactggaagc aggcgcttgg gttttcgctc atcgaggggc tgatgccgct ggcggcctac
     2161 agcacgctgc gctactacgg cggccttggc gacagcgcgg gagtcgggta ttacgtgctc
     2221 ttcggcttct gcgcgctggt catcctcttt gcgctgctca tgcgtcagct tgtgtatacg
     2281 ctgacggtga cttacgagct caaattcggg cagatcgtta aaaacgcgtt tctgctgacg
     2341 tttcttgaac tgccaaagag cctttggacg ctgctgaaaa acagtctgcc cgtcgcggtt
     2401 tttgcgcttt tactctggct gctgcctgcg tatacggggc tgatcgtgat tcttgggctg
     2461 gcctactacg cgctgttcgg ggtcgcgctg gagcggttcg tttcggcttc gtttgccaac
     2521 tacgttgtgg aaaagcatat caattcaaag ctttccggcg cgcgcgtgga catcggcatg
     2581 gcgagcgagc gggtggtttc gtctgagtcg tcgcaggaac gagagtaaca gataaacaga
     2641 tacagtaaag gtcatccgaa aacggatgac ctttatttgc ataatggtga attatactat
     2701 caagtgcaac actttaggga aataaagaag ttcatgcaag
//
LOCUS       DAOMGY010000158         2738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_40264_length_2738_cov_3.459933, whole genome shotgun sequence.
ACCESSION   DAOMGY010000158 DAOMGY010000000
VERSION     DAOMGY010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40264_length_2738_cov_3.459933"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..875)
                     /locus_tag="PLR57_03290"
     CDS             complement(84..875)
                     /locus_tag="PLR57_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HQQ40510.1"
                     /translation="MKKGIYTWFGFEIPARERLVAIREAGFSAIMLNWEDGLPEMRHK
                     EGYADLARELDLEIVNGHLPYEGANDLWRDGLDGDDWMEFLIQSILSAGRSGVPVLVL
                     HAARGSTPPSLSEIGFTRFQRLNDAAEQANVVLAIENLHSLSYSMAVLDALDTPFVRA
                     CYDAGHHNCYGQDDAFLDRFGARLCSIHLHDNDGRHDQHLLPFTGTIDWPAEMARIKK
                     TAYRGPITLELGGTPELPMEEHLLAASAAADRLIALWEAAETDSI"
     gene            complement(936..1589)
                     /locus_tag="PLR57_03295"
     CDS             complement(936..1589)
                     /locus_tag="PLR57_03295"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ40511.1"
                     /translation="MRDAYWEGVWQAEDAAQCAEYLRGHMRARPAFLDVFRQYGAADV
                     CDAACGFGAYGAMLTANGFSVSLFDIASSSIALAKTLFQREGLAAKDWQVCDICQISY
                     PSGSFDAVVAHAVIDHLSTERARTALDELTRIAKPDGLVYLSFDPLEEDDLLEPHDIL
                     PDGSFLYTGGSRKGLLFHPYSEADAQQFLLGQKIVYSRVSSRGEREYVLQTTQTSAE"
     gene            complement(1595..2155)
                     /locus_tag="PLR57_03300"
     CDS             complement(1595..2155)
                     /locus_tag="PLR57_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40512.1"
                     /translation="MTRPRSPVRRHIAHYLKRNLILAFACAGPIAVLFAVVGIVYKSN
                     LTDIWTGIGLAFALAAMFYFAGALACIRFVRMIRLQEALFETEFDDQNATVLDRIGLT
                     SVSDRWFIRSGSAAFYDRYIRSIRYRTLHSQSGVGYRVTVLTTDGKSYGLWLNSVSDI
                     KRAQAWLAERRRQFACEADMPEEAKG"
     gene            complement(2152..2694)
                     /locus_tag="PLR57_03305"
     CDS             complement(2152..2694)
                     /locus_tag="PLR57_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HQQ40513.1"
                     /translation="MEPVFINHCTLNKKNLAEMAKHTRRGARVIVNICSVLLLLVALT
                     EYFVIYDFSTAFFALFLGVFFFAYVQLLPRISARMTLKRYQVLYHAEVVSELQFFEDH
                     VLMQSEQSKEHIELQYAQIKKVIRTKNLYLLQLGAQLILLTDKTGFANGASCSAFEGF
                     IRQKASCAKFRFPRQTRVKE"
BASE COUNT          579 a          785 c          801 g          573 t
ORIGIN      
        1 ttccgtgttt gcgtcgtagt aggcttttac tgtgctgttc gcgtcttcca tagaacactc
       61 cttaaaaact gagctatcga atactaaata ctatctgttt ccgccgcttc ccagagcgca
      121 atcagccggt cggctgcggc gctggcggca agcagatgct cctccatagg cagttccggg
      181 gttccaccga gctccaacgt aattggcccg cggtaggcgg tctttttaat gcgcgccatt
      241 tcggccggcc agtcaatcgt acccgtaaac ggcagcaggt gctggtcgtg cctgccgtcg
      301 ttgtcgtgca ggtgaatgga gcaaagccgc gcgccgaagc ggtcgagaaa cgcgtcgtcc
      361 tgaccgtagc agttgtgatg tcccgcgtcg taacaggcgc gcacaaacgg ggtgtccagc
      421 gcgtccagca ccgccatact gtaggaaagc gaatgcaggt tctcaatggc gaggacgacg
      481 tttgcctgct ctgcggcgtc gtttagtcgc tgaaagcgtg tgaagccaat ctcggaaagc
      541 gacggcggcg tgcttccgcg cgcggcgtgc agaacgagta ccggaacccc ggagcgcccc
      601 gcggagagga tgctctgaat caggaactcc atccagtcgt ctccgtccag tccgtcgcgc
      661 cagaggtcgt ttgcaccttc gtacggcagg tggccgttga cgatttccag atcgagttcg
      721 cgggcgaggt cggcgtaacc ctccttgtgc cgcatctccg gcaggccgtc ctcccagttc
      781 agcataatgg cggaaaagcc tgcttcacgg atggctacca gccgctcgcg cgcgggaatt
      841 tcaaaaccaa accaagtata aatacccttt ttcaaacgaa ctgcctttcc tgtttgccgg
      901 ctgcgggacg gttccgcagg ccgttatagt tgcgcttact ccgcgcttgt ttgcgttgtt
      961 tgcaggacat attcccgttc gcctcgtgaa ctgacgcggg aatacacgat tttttgcccg
     1021 agcaggaact gctgcgcatc cgcttcggaa tacgggtgga acaacagccc cttgcggctc
     1081 ccgccggtat agagaaaact tccgtcgggg agaatgtcgt gcggctcgag aaggtcgtct
     1141 tcttccagcg ggtcgaagga aaggtacaca agcccgtcgg gttttgcaat gcgcgtcagt
     1201 tcgtcaagcg ccgttcgcgc gcgttcggtc gagagatggt cgattaccgc gtgggcaacg
     1261 accgcgtcga aactgccgct cgggtaggag atctggcaga tatcgcagac ctgccagtct
     1321 tttgcggcaa gcccttcccg ctgaaacaga gtttttgcaa gcgctatgga actgcttgcg
     1381 atgtcgaaga gtgaaacgga gaaaccgttc gcggtcagca tcgctccgta cgcaccaaag
     1441 ccgcaggccg cgtcgcacac gtctgccgcg ccgtactgac gaaacacgtc cagaaacgcg
     1501 gggcgggcgc gcatatgccc gcgcaggtat tcggcgcact gtgcggcgtc ctccgcctgc
     1561 caaacgcctt cccagtaagc gtcgcgcatg atcgttatcc tttcgcttcc tcaggcatat
     1621 ccgcttcgca cgcaaactgc ctgcggcgct cggcaagcca ggcctgcgcg cgcttgatgt
     1681 ccgaaacgct gttgagccag agaccgtagc tctttccgtc cgtggtcagc accgtaaccc
     1741 ggtagcctac gccgctttgc gaatgcagcg tgcggtagcg gatggaacgg atgtaccggt
     1801 cgtaaaacgc tgccgaaccg gagcggatga accagcgatc cgaaacagag gtcagcccta
     1861 tgcggtcgag cactgtggcg ttttgatcgt caaattctgt ctcaaagagc gcttcctgca
     1921 ggcggatcat gcgtacaaag cggatgcagg cgagagctcc cgcaaagtag aacatggccg
     1981 caagcgcaaa ggcaagtccg atacctgtcc agatgtcggt gagattcgat ttgtaaacga
     2041 tgccgacaac ggcaaacagc acggcaatcg gccccgcgca ggcgaacgca agaatgaggt
     2101 tgcgcttgag atagtgcgca atatgccgcc ggacgggcga acgcgggcgt gtcattcctt
     2161 tacgcgcgtc tgccgcggga atctgaactt cgcgcaggag gccttttgac ggataaatcc
     2221 ttcaaaagcc gagcaggacg cgccgttggc aaatcccgtc ttgtccgtca gcagaataag
     2281 ctgtgcgccg agctgcagca ggtagaggtt cttggttcga atcacctttt taatctgcgc
     2341 gtactggagt tcgatatgct ccttcgactg ttcgctctgc atcagaacat gatcctcaaa
     2401 gaactgaagc tcgctgacga cttcggcatg ataaagcacc tgatagcgtt tcagcgtcat
     2461 gcgcgcggag atgcgcggca gaagctgtac ataagcaaag aaaaacacgc caagaaacag
     2521 cgcgaagaac gccgttgaaa aatcatagat cacaaaatac tcggtcagcg cgacgagcag
     2581 cagcaaaacc gagcagatgt ttacgatgac gcgagcgccg cgccgggtat gcttcgccat
     2641 ttcggcgagg ttctttttgt tgagcgtgca gtggttgatg aaaaccggtt ccatgaaacg
     2701 tctccttttg tgattgcgct ttcgcgcttt ttatccgc
//
LOCUS       DAOMGY010000159         2737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_40289_length_2737_cov_2.993289, whole genome shotgun sequence.
ACCESSION   DAOMGY010000159 DAOMGY010000000
VERSION     DAOMGY010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40289_length_2737_cov_2.993289"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PLR57_03310"
     CDS             <1..855
                     /locus_tag="PLR57_03310"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ40514.1"
                     /translation="YEPITETIRQTEGRPLHFAHLQFYGYSRSADGKMTSAAERLAEL
                     VNANKNITCDVGAVTFGPAAAVTADANFAKKLGRGKRGFQSQWWEQDGAFGVLPLKYE
                     ADKIAGATQFLTGLELMLLVEDASRIFLTTDHPNGGPFTDYPYLIQLLMDKPFRDEEI
                     HKLNPKAVSQSAAPSIAREYSLYEIAQITRSGPAAALGMRARGHLGVGAEGGVAVYRE
                     QADKRAMFSAPAAVFRGESAVLVPMERAFDEDWVERRTEGLRSGNPFEARMDEDFLQT
                     HRIARQEA"
     gene            859..1599
                     /locus_tag="PLR57_03315"
     CDS             859..1599
                     /locus_tag="PLR57_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40515.1"
                     /translation="MGVILMLKNPLETLLDLAPLSELVNADSEELANAKLAYGREFVF
                     AVELFTAAPSSEEGLTLNGNLHLCINAGAGLREGKMTIASSVGASAGEGMLGGKLIVH
                     GSVGKNACREMQNGFVRIYGDAENGLARGMRRGMIVVSGRAHGETCAALRGGTVLLLS
                     GTDEPEQLARGLSRGTVILPKGACVPSGFSRAASLDYTFLRLLFLELEQRGEKLPLGW
                     LGGVFTRYRGDAAGLGKGEIFVLSEERV"
     gene            1596..2447
                     /locus_tag="PLR57_03320"
     CDS             1596..2447
                     /locus_tag="PLR57_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HQQ40516.1"
                     /translation="MKHIAVLGTRLGWHCQEVLRAFERRGAEADFVTIDRLVCPLGLP
                     EAKLPLVEYDGVMIRGIPTGSLEQVIYRMDALFALERAGLLCVNSPKTIEKTVDKFLT
                     SRLLEEAGLPVPPTICCETSDAAFEAFAVLGGDVVYKPLFGSCGNGLLRLRTVEEAKE
                     AFAPLTEAGSVLYLQKFIPSGNSDIRAFVIGGRVVASMRRRGKDWRANVSRGGTAEAY
                     KLDIWQETLALAAAGTVNAAVAGVDLLVSESGETYVTEVNGCPGWQGLSTVTDVDIAD
                     EMVSLFF"
     gene            complement(2540..>2737)
                     /locus_tag="PLR57_03325"
     CDS             complement(2540..>2737)
                     /locus_tag="PLR57_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40517.1"
                     /translation="PEMPMRLMIDLIEEIEPAGPYGAKSIGECSVVPAAAAIANAVSN
                     AIGRDCNDLPVTPERILEKLK"
BASE COUNT          535 a          732 c          920 g          550 t
ORIGIN      
        1 tacgagccga ttacggaaac gatccggcag acggagggca ggccgctgca ctttgcgcac
       61 ctgcagtttt acgggtattc gcgctcggcg gacggaaaga tgacttccgc cgccgaacgg
      121 ctggcggaac tcgtcaacgc gaacaagaac atcacctgcg acgtcggcgc ggtgacgttc
      181 ggccccgcgg cggcggtcac ggcggacgcg aactttgcca agaaactggg gcgcggcaaa
      241 cgcggcttcc agagccagtg gtgggagcag gacggcgcgt tcggcgtgct cccgctcaaa
      301 tacgaagcgg acaaaatcgc gggcgcgacg cagtttttaa ccggactgga gctcatgctt
      361 ctggtggagg acgcttcccg tatcttttta accaccgacc atcccaacgg cgggccgttt
      421 accgattatc cgtatttgat tcaacttttg atggataagc cgtttcggga tgaagagatt
      481 cataagctga acccgaaggc ggtttcgcag agcgcggcgc cttcgattgc gcgcgaatat
      541 tcgctctacg aaatcgcgca gattacgcgc tccggccccg ccgcagcgct gggtatgcgc
      601 gcgcgcgggc atcttggcgt cggcgcggag ggcggagttg ccgtctaccg cgagcaggcg
      661 gacaagcggg caatgttcag cgcgcccgcg gcggtctttc gcggagagtc cgccgttctc
      721 gttccaatgg aacgcgcgtt cgacgaagat tgggtcgaac gccgtacaga aggtttgcgc
      781 agcggcaatc cgttcgaagc gcggatggac gaagattttc tgcagactca ccgcatcgca
      841 aggcaggagg cgtaaccgat gggcgtgata ttaatgttaa agaatcctct ggaaacgctg
      901 ctcgatctgg ctccgctttc cgaactggtc aacgccgact cagaggaact ggcgaatgca
      961 aaacttgcat acgggcggga attcgttttc gcggtcgagc tttttaccgc cgcgccgtcc
     1021 tccgaggaag ggctgacgct gaacggcaat ctgcatctct gcattaacgc gggagcggga
     1081 ttacgcgaag gaaaaatgac gattgcgtcc tccgtcggcg cgagcgcggg cgaaggaatg
     1141 ctgggcggca aactgattgt gcatggcagt gttggcaaaa acgcctgccg cgagatgcaa
     1201 aacgggtttg tgcggattta tggcgatgca gaaaacggtt tagcgcgcgg tatgcggcgc
     1261 gggatgatcg ttgtttccgg cagagcgcat ggcgaaacct gcgctgcgct tcgcggagga
     1321 acggtgcttt tgctgagcgg aacggacgag ccggaacagc ttgcgcgggg gctttcgcgc
     1381 gggacggtca tcctgcccaa gggcgcatgc gttccaagcg ggttttcacg cgcggcgagc
     1441 ctcgactata cctttttgcg gctgcttttt ctggaactcg aacagcgcgg agaaaaactt
     1501 ccgctaggct ggctcggcgg tgtgtttacg cggtatcgcg gggacgcggc ggggctgggc
     1561 aaaggggaga tcttcgtgct ttcggaggaa cgtgtatgaa acacattgcc gtactcggca
     1621 cgcgcctcgg ctggcattgc caggaagtgc ttcgcgcgtt cgagcgcaga ggagccgagg
     1681 cggattttgt gaccatcgac cggctggtct gcccgcttgg tctgccggaa gccaaactgc
     1741 cgctcgttga atacgacggg gtgatgatac gcgggattcc gacggggtcg ctcgaacagg
     1801 tgatctaccg catggacgcg ctgtttgcgt tggaacgcgc aggtctgctt tgtgtgaatt
     1861 caccaaaaac gatcgaaaaa acggtcgata agttccttac gtcgcggctg ctcgaagaag
     1921 cggggctgcc cgttccgccg acgatctgct gtgaaacgtc ggacgcggcg tttgaggcgt
     1981 tcgccgttct cggcggggac gttgtgtata agccgctgtt tggttcctgc ggaaacgggc
     2041 tgctgcgcct gcggacggtg gaggaagcaa aagaggcgtt tgcgccgctg acagaggcgg
     2101 ggagtgtact gtacctgcag aagtttatcc cgagcggaaa cagcgatatc cgcgcgttcg
     2161 tcattggcgg gcgggtggtt gcgagcatgc ggcgcagggg caaggactgg cgggcgaatg
     2221 tttcgcgagg gggaactgcg gaagcctaca agctcgatat ctggcaggag acgctcgcgc
     2281 tcgcggcggc aggcacggtg aacgcggcgg tcgccggggt cgatctgctg gtttcggaat
     2341 ccggcgaaac gtatgtgacc gaggtgaacg gctgtcccgg ctggcagggg ctttcaacgg
     2401 tgacggacgt ggacatcgcc gacgagatgg tctcactctt cttttagcaa atttggcata
     2461 gcaaaagccg ggcttacgcc cggcttattt aatagctaag agagttttct gcttcttttt
     2521 ttcaaaagag aagcgtactc tatttcagct tctccaaaat ccgctccggc gtgaccggca
     2581 ggtcgttgca gtcacgcccg atggcgttgg aaacagcgtt tgcgatggcc gcggcggcgg
     2641 gaacgacgga gcactcgcca atgctcttgg cgccatatgg ccccgcgggc tcaatctctt
     2701 caatcaagtc gatcatcagc cgcatgggca tttcggg
//
LOCUS       DAOMGY010000160         2730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_40440_length_2730_cov_3.353645, whole genome shotgun sequence.
ACCESSION   DAOMGY010000160 DAOMGY010000000
VERSION     DAOMGY010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40440_length_2730_cov_3.353645"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..772
                     /gene="mazG"
                     /locus_tag="PLR57_03330"
     CDS             5..772
                     /gene="mazG"
                     /locus_tag="PLR57_03330"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HQQ40518.1"
                     /translation="MEDVMRVVRRLRAPDGCPWDREQTHASLKNALLEECYELIDAID
                     EGDDVHICEELGDVLLQFALHAVIAEEHGAFTAQDAATDLVEKLIYRHPHVFGEVRVS
                     GSGEVLKNWDALKMQQRKQETQAEVLKSVPRSFPALLRSRKVQKKAADVGFDWPNAGE
                     AFFKIGEETTELEEAMHSGEHVAEEMGDLLFAVVNVARLLKLEPEFLLMEATDKFIGR
                     FETMEQLVVSSGKALSEMTLEQMDMFWEEAKKRGNRE"
     gene            852..1124
                     /locus_tag="PLR57_03335"
     CDS             852..1124
                     /locus_tag="PLR57_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQQ40519.1"
                     /translation="MNKTDLIAAVAEKSELSKKDAEKAVGALLSAIEDSLKAGEKVSI
                     VGFGTFEAKVRPARKGHNPQTRKEIDIPASKLPTFKAGKALKDSLN"
     gene            1340..1915
                     /locus_tag="PLR57_03340"
     CDS             1340..1915
                     /locus_tag="PLR57_03340"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008537362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HQQ40520.1"
                     /translation="MRQFQDRIRAEGRVLPGNIIKVDGFLNHRVDTALMRDMAKEFKS
                     RFDLSGLTAVLTVEASGIALATICAEEFGVPMIFAKKAKSDNIEGGLYHSEIYSYTYK
                     KKFTLILASQWLKAHDKVLIIDDFLANGEALRGLIEIVHEANADLIGIGVAIEKGFQP
                     GGKNIREAGIHLESLAIIESADETGIRFREE"
     gene            1919..>2730
                     /gene="guaA"
                     /locus_tag="PLR57_03345"
     CDS             1919..>2730
                     /gene="guaA"
                     /locus_tag="PLR57_03345"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQQ40521.1"
                     /translation="MRERVVVLDFGGQYNQLIARRVREAGVFSELIPHSASIERIKGD
                     SLSAIILTGGPDSVYAPDAKSCDNAIFSLGVPVLGICYGMQYIAKVFGGEIASANIRE
                     YGHTKIQLDAHPLFNGLGGVVWMNHNDSVTRAPEGFTAIARTDACPVAAFGDEQRKLY
                     GIQFHAEVTHTVNGQLLFRNFLRDVCGLRCDYSAANLREELIAQIRAQVGSARVIGAL
                     SGGVDSSVASVLVHEAIGEQLTCIFVDHGLLRENEAEEVMRVYHDTLSLNIV"
BASE COUNT          664 a          691 c          810 g          565 t
ORIGIN      
        1 ttcgctggag gacgtcatgc gcgttgtgcg ccgtctacgc gcgccggacg gctgcccgtg
       61 ggaccgcgag cagacgcacg cgtcactgaa gaatgcgctg ctggaagagt gctatgagct
      121 catcgacgcc atcgacgaag gcgacgatgt gcatatctgc gaggagcttg gggacgtgct
      181 gctccagttt gcgctgcacg ctgtaatcgc ggaagagcat ggggcgttta ccgcgcagga
      241 cgcggcgacg gatctcgtag aaaagctgat ctatcgtcac ccgcatgtgt tcggcgaagt
      301 acgcgtcagc ggttccggcg aagtgctgaa aaactgggac gcactaaaga tgcagcagcg
      361 caagcaggaa acccaggcag aagtgcttaa aagtgtaccg agaagctttc ccgcgctgct
      421 gagaagccgc aaggtgcaaa agaaagcggc ggacgtcggc tttgactggc cgaacgcggg
      481 ggaggcgttc tttaagatcg gtgaggaaac cacggaactg gaagaagcga tgcattccgg
      541 cgaacatgtt gccgaggaga tgggcgatct gctgttcgcg gtggtcaacg ttgcgcgact
      601 tttgaaactc gagccggaat tcttgttaat ggaagcgacg gataagttta tcgggcgctt
      661 cgaaacaatg gagcagctcg ttgtttcaag cggaaaagcg ctgtcggaaa tgacgctcga
      721 acagatggat atgttttggg aagaagcaaa aaagcgcgga aacagggaat aattcacaaa
      781 ttaaggcttg ataaatacca cgaagattga tacaatgttc cagcaaccaa aatactatgg
      841 aggtttacac aatgaataaa accgatttaa tcgcggctgt cgcggagaag agcgaactgt
      901 cgaaaaagga tgcggaaaaa gccgttggtg ctctgctgag cgcgatcgaa gattccctaa
      961 aggccggtga aaaggtatcc atcgtcggtt tcggcacgtt cgaagcgaag gttcgccccg
     1021 ctcgtaaggg ccacaacccg cagacccgca aagaaatcga tattcccgct tccaagctgc
     1081 ccaccttcaa ggccggcaaa gccctcaaag attcgctcaa ctaaaagaag agtcgctttc
     1141 ttacgccgta cggttcaccg tgcggcgttt ctcttgtgcc aagaagcggc ggttaaattt
     1201 tacaaagaga actgctaaca ggcagataaa atggaatcca agcaaacaga tttaccaatc
     1261 ttgctgtcgg ggcttgattc ctggctccat ttgcgagata atgtttgcat tattttactt
     1321 tgacgggaga tgatgcggta tgcggcagtt ccaggaccga atccgcgccg aggggcgcgt
     1381 acttccgggc aatatcatca aggtagacgg ctttttgaat caccgcgtgg atacggcgct
     1441 gatgcgcgac atggcgaagg aattcaagag ccgttttgat ttgtccgggt tgacggcggt
     1501 gctaacggtg gaagcctccg gcattgcgct ggcaacgatc tgcgcggaag aattcggtgt
     1561 tccgatgatt tttgcgaaaa aggcaaaatc ggacaatata gagggcggcc tctaccacag
     1621 cgaaatctac tcctatacct ataaaaagaa gtttacgctg attctcgcca gccagtggct
     1681 caaggcgcac gacaaggtgc tgattattga cgattttctg gcaaacggcg aagcgctgcg
     1741 cggcttgatt gaaattgtcc acgaagcgaa cgcggatttg atcggcatcg gcgtggcgat
     1801 tgaaaaagga ttccagcccg gcggaaaaaa tatccgcgag gcgggaatac atctcgaatc
     1861 tttagctatc atcgaaagcg ccgacgaaac cggaatccgg ttcagggagg agtaactcat
     1921 gcgcgagcgc gtcgtcgtcc tcgactttgg cgggcagtat aaccagctga ttgcgcggcg
     1981 tgtgcgtgaa gcgggcgtat tcagcgaact gattccgcat tccgcttcga ttgaacgcat
     2041 caaaggggac tctctttcgg ctatcatcct caccggcggg ccggacagcg tgtacgcgcc
     2101 ggacgcaaag tcctgcgata acgctatctt ttcgctgggc gttccggtgc tgggtatctg
     2161 ctacggcatg cagtacatcg ccaaggtatt cggcggggaa atcgcttcgg caaacattcg
     2221 cgaatatgga catacaaaaa tccagctcga cgcgcacccg ctctttaacg gacttggcgg
     2281 cgtggtctgg atgaaccata acgactccgt aacgcgcgcg ccggaggggt ttaccgccat
     2341 tgcgcggaca gacgcctgcc cggttgcggc gtttggcgat gaacagcgca agctctacgg
     2401 catccagttc cacgcggaag tgacgcatac cgtcaacgga cagctcctgt tccgcaactt
     2461 cctgcgggac gtttgcggct tacgttgcga ctacagcgcg gctaacctgc gcgaagaact
     2521 gattgcgcaa atacgcgcgc aggtaggctc tgcccgcgtg attggcgcac tctccggcgg
     2581 cgtggactct tcggtagcgt ccgtgctcgt gcacgaggcg attggcgaac agctcacctg
     2641 catctttgtg gatcacggcc tgctgcgcga aaacgaagcg gaagaggtta tgcgcgttta
     2701 tcacgatacg ctctcgctca acatcgtcaa
//
LOCUS       DAOMGY010000161         2721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_40644_length_2721_cov_3.822956, whole genome shotgun sequence.
ACCESSION   DAOMGY010000161 DAOMGY010000000
VERSION     DAOMGY010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40644_length_2721_cov_3.822956"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLR57_03350"
     CDS             complement(<1..512)
                     /locus_tag="PLR57_03350"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HQQ40522.1"
                     /translation="MLAGFLFGYAYRRNVAEAKIAKAEDAVKKLYDDAQRKAEEVKKE
                     KILEAKEEVFRIRSDAERENRERRAEVQRNERRIFQREEILDKKSDTLEKRELALAER
                     ETELETTQAEIKALHDQQLKELEKISGLSMEAAKDMLLANVEHEARHEAAILLRDIES
                     KTKEEADKRAR"
     gene            complement(765..>2721)
                     /locus_tag="PLR57_03355"
     CDS             complement(765..>2721)
                     /locus_tag="PLR57_03355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF001688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HQQ40523.1"
                     /translation="QARLDRELKSIIGNGFASLYLMAQRLVHKSNSDGYLVGSRGSVG
                     SSFVATMAGITEVNSLQPHYVCPHCKHSDFEVDRVKYACGIDMPQKDCPVCGTPYLKM
                     GFDIPFEVFLGFDGDKTPDIDLNFSGEYQGTAHKYVEEMFGRGHAFRAGTISGVAVRT
                     AFEYVRIYEEKNGRTLRKAERERIANGCQDVKVTTGQHPGGIVIVPKEDDILDYTPIQ
                     YPADKRDKAIITTHFDFHAMDDRLVKLDILGHDDPTALRMLQDLTGLDPVTIPLDDPE
                     TMRIYTSSEPLKVDLSALRCSVGTLGTPEFGTSFVRGVLEATMPTTMEELVRISGLTH
                     GTDVWLGNAETLVANKTATLSEVICTRDDIMNYLILRGVDASSSFKIMESVRKGKGLK
                     PEMEQKLLDNDIPAWYIDSCKKIKYMFPRGHAVAYTMMSFRVAYYKVHYPLEFYSVYY
                     TVRADLFDVSLAYGGAEQVLERIREIDRSTAKMSDSEKKRTGDIVTILEVVYEMNMRG
                     IELLPVDLYKSHATRFLIEDGAIRPPFNAIPSVGDNAAIDMAANRRNHVFHTIDEFRR
                     ITKANSAVISAMERCGCFAGMSRTDQISLFSFDDVEEEPEKRETVVPEQEESGDEEPL
                     FVQSDSEPETGDFFPDEFEDDSEDDEI"
BASE COUNT          565 a          756 c          697 g          703 t
ORIGIN      
        1 cgcgcgcgct tgtccgcttc ttccttggtt ttgctttcaa tgtccctcag caggattgcc
       61 gcttcatgcc gcgcttcgtg ctcgacgttt gccagcagca tatcctttgc cgcttccatg
      121 gagaggccgg aaatcttttc cagttcctta agctgctgat cgtgcagggc tttgatttcc
      181 gcctgcgttg tttccagctc ggtttcgcgc tcggcgagag ccagttcgcg cttttccagc
      241 gtgtcggact ttttgtccag aatctcttcc ctctggaaaa tgcgtctttc gttgcgctgg
      301 acttccgcgc ggcgttcccg gttttcacgt tccgcatcgg aacggattcg aaatacttct
      361 tcttttgctt cgagtatctt ttctttttta acttcctcgg ccttgcgctg cgcgtcgtca
      421 tacagtttct tgaccgcatc ttcggccttt gcaattttcg cttccgctac attacgccgg
      481 taggcatatc caaacaggaa accggcgagc aatgcgaagg cgcctattgt taggtataac
      541 caccacatat gattgctccg atctataaaa aatgtaaccg cacatctctg cacggtcact
      601 tcaaatgaat cattcctcat cacatagttg taaaaaacga tttattatga acacaccata
      661 agagtgcagg attactccaa aaaattatat atcaacaaag cgtgcgcgtc aagtatttag
      721 cggcttttca gacgtttctc acgtcaggat tgaacgccct gaagctaaat ttcgtcatct
      781 tccgaatcgt cttcaaattc gtctgggaaa aagtctcccg tttccggttc ggaatcgctt
      841 tgcacgaata acggttcttc atcgccgctt tcttcctgtt ccggcacaac cgtctccctt
      901 ttttcaggtt cttcttcaac gtcgtcaaag ctgaagagtg aaatctggtc ggttcgggac
      961 atgccggcaa agcagccgca cctttccatt gccgaaatca ccgcgctgtt cgctttcgta
     1021 atgcggcgga actcgtcaat ggtgtgaaac acgtgatttc tccggtttgc cgccatatcg
     1081 atggccgcgt tgtccccaac gctggggatt gcgttaaacg gaggccgaat cgcgccgtct
     1141 tcaatcaaga agcgcgttgc gtgcgattta tagagatcga ccggcagcag ttcgatgccg
     1201 cgcatgttca tttcgtacac cacttccaaa atggtgacga tatctccggt gcgtttcttt
     1261 tcggaatcgc tcatctttgc cgtcgagcgg tcgatttcgc gaatgcgctc cagaacctgt
     1321 tccgcgccgc cataggcaag cgaaacatcg aacaaatccg ctcgtacggt ataatataca
     1381 ctgtagaatt ccagcgggta atgcacttta tagtacgcca cgcggaagga catcatggta
     1441 tacgcaacgg cgtgcccgcg cgggaacatg tatttaatct ttttgcagga gtcgatgtac
     1501 caggcgggaa tgtcgttatc gagcagtttt tgctccatct ccggcttgag ccctttgccc
     1561 ttacggacgg actccatgat cttaaacgag ctggaagcgt ccacgccgcg cagaatgagg
     1621 tagttcataa tgtcgtcgcg tgtgcagatg acctccgaaa gcgtcgcggt tttgttggca
     1681 accagagttt ccgcgttgcc gagccaaacg tccgtaccgt gcgtcaggcc ggagatgcgg
     1741 acgagctctt ccattgtggt tggcattgta gcttccagca cgccgcgcac aaagcttgtg
     1801 ccgaactccg gcgtgccgag cgtacccacc gagcagcgca gcgccgaaag atcgaccttg
     1861 agcggctcgc tggaggtgta gatccgcatc gtttccgggt cgtccaacgg aatggttacg
     1921 gggtcgagcc cggtgaggtc ctgcagcata cgcagagcgg tcgggtcgtc gtgaccgagg
     1981 atatccagct tgacgaggcg gtcgtccatg gcgtggaagt cgaaatgcgt cgtaataatc
     2041 gctttatcgc gcttatccgc cgggtactga atgggtgtat agtcgaggat gtcgtcttct
     2101 ttcggcacga tgacgatacc gccggggtgc tgccccgtag tcaccttaac gtcctgacag
     2161 ccgttggcaa tgcgctcgcg ctcggctttt ctgagcgttc gcccgttttt ctcttcgtag
     2221 atgcggacgt attcaaacgc cgtgcgcacc gccacgccgg aaattgtacc cgcgcggaag
     2281 gcatggcccc gcccaaacat ctcttcaacg tacttatgcg ccgtgccctg atattcgccg
     2341 gaaaaattca ggtcgatatc cggcgttttg tcgccgtcga agccaaggaa cacttcaaaa
     2401 ggaatgtcaa accccatctt gagatacggc gttccgcaga caggacagtc cttttgcggc
     2461 atgtcgattc cgcaggcgta tttcacgcgg tctacttcga agtcgctgtg cttgcagtgc
     2521 gggcagacat agtgtggttg aagcgagttt acctccgtaa tgcccgccat agtcgcgaca
     2581 aatgaggagc cgaccgaacc acgggaacca acaaggtagc catcggaatt actcttgtgt
     2641 accaatcgct gcgccatgag atagagcgaa gcaaatccgt tgccaataat ggatttcagc
     2701 tctcgatcaa gtcgcgcctg c
//
LOCUS       DAOMGY010000162         2712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_40869_length_2712_cov_3.361686, whole genome shotgun sequence.
ACCESSION   DAOMGY010000162 DAOMGY010000000
VERSION     DAOMGY010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40869_length_2712_cov_3.361686"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLR57_03360"
     CDS             complement(<1..369)
                     /locus_tag="PLR57_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HQQ40524.1"
                     /translation="MRRIRVALWGFGAMGGGIARVLLGKQGVEITGVCDLHPNRVGKS
                     IFELLEIERGDQADVLIQEDIDAVLPKGGADICIVATDSFTRDVYPKLLRVIKSGINV
                     ICTAEEMSYPAAKEPKLAAEI"
     gene            complement(457..1209)
                     /locus_tag="PLR57_03365"
     CDS             complement(457..1209)
                     /locus_tag="PLR57_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQQ40525.1"
                     /translation="MQYYDLHTHTSMSDAGYPLSELVEVEKKLGNILGVSDHLFCCGI
                     YTLRDLSDYLAELSNYNVYRGVEVNMEHHITLPDAMDDQIDYVIASVHSMPDGRGGFV
                     PLNAYFSKRSGHTLEYNKNYSSDLNRYYLAYIYQMIEKAFSNERIDILGHGTVLPPCD
                     ELHGTKFLLQWEDAIIGLCKKHGIAMEISGLWHAPNMEMILRAKEAGVKFSMGSDCHN
                     RLQIGDLAYVERAVEEVGLTQEDFFVPRRALD"
     gene            complement(1391..2386)
                     /locus_tag="PLR57_03370"
     CDS             complement(1391..2386)
                     /locus_tag="PLR57_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HQQ40526.1"
                     /translation="MKRMLEAILAASTSGRSAMLCTIVSRRGTTPRGVGTAMVVDENG
                     AQTGTVGGGEAEYQVKQDALALLRENVSALRSYAIHPGEGEGCAVSGGEIDVLLRLFT
                     GESARKLAERALEVLDREEGYLVCSIDHGTVGETEVVPAWAAKDDPALKPFLSAAPVL
                     GTEEPRRFIEPLYPPPRAVILGGGHVAKALVPVLAQLGLRVWVVEGRDTLCDSSRFPM
                     AERVLSEMQERFLDELELTERDAVISMVRGRGIDFFILDAALRTRAGYIGAIGSHAHA
                     KKMRDQLLSRGFSKEQIARIHSPVGLDIGAESPEEIAVSIAAEWIACRYGKEKTR"
     gene            complement(2454..>2712)
                     /locus_tag="PLR57_03375"
     CDS             complement(2454..>2712)
                     /locus_tag="PLR57_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40527.1"
                     /translation="IHDIANELKEQNISYSLELFEVEHGQPFASLEDACAFFSFYSRR
                     ECSAEEVEPLLSKTNDPALPLYFAKTRKLGLLIFEAGAKKN"
BASE COUNT          583 a          827 c          698 g          604 t
ORIGIN      
        1 gatttccgcg gcgagcttgg gttcctttgc ggcgggatag ctcatctcct ccgccgtgca
       61 gatgacgttg attccgctct tgatgacgcg aagaagcttc gggtagacgt cccgcgtaaa
      121 cgagtccgtc gccacaatgc agatgtccgc gccgcccttt ggcagcaccg cgtcgatatc
      181 ctcctgaatc agcacatcgg cctgatcgcc gcgctcaatt tccagcagtt caaagatgct
      241 cttgcccacg cggttcgggt gcaggtcgca aacgcccgta atctcgacgc cttgcttgcc
      301 aagaagcacg cgcgcaattc cgccgcccat cgcgccaaat ccccagagtg caaccctgat
      361 tctacgcata ctcgttacaa tcctcccggt ttaacccgta ctctattcga tttacagtgt
      421 ctttagaaac tagaatgaaa atccgacgat aagaaactaa tccagcgctc tgcgcggaac
      481 aaagaaatct tcctgtgtta agcctacctc ttcgacggcg cgctccacat aggccaagtc
      541 accgatctgc aggcggttgt gacagtcgct gcccatagaa aacttcacgc ccgcctcttt
      601 ggcccgcaga atcatctcca tattcggcgc atgccaaagc ccagaaatct ccatggcgat
      661 gccgtgcttt ttgcataagc cgataattgc atcctcccac tggagcagga actttgtgcc
      721 gtgcaattcg tcgcagggcg gcagaaccgt accgtggccg agaatatcga tccgctcgtt
      781 ggaaaacgct ttttcaatca tctggtagat gtaggccaga tagtagcggt ttaggtcgga
      841 agagtagttt ttattatatt cgagcgtatg cccggaacgc tttgaaaaat acgcgttgag
      901 cggaacaaac ccgccgcgcc cgtccggcat gctgtggacg cttgcaatca cgtagtcgat
      961 ctggtcgtcc atcgcgtccg gcagggtaat atggtgctcc atattgactt ccacgccgcg
     1021 gtaaacgttg tagttggaga gctccgccag atagtccgaa aggtccctca acgtatagat
     1081 gccgcagcag aagagatggt cggaaacgcc gaggatatta cccagctttt tttcgacctc
     1141 caccagttcc gagagcgggt atcccgcgtc ggacatgctc gtgtgggtat gcaggtcgta
     1201 atattgcatg gggttctccg tttccagttg attgcataaa gaaggctcag tctatcgttc
     1261 tatgcatcct ttgcgcccta ccaatatacc atgatcgcgg cagcgaaaca agggcgtgat
     1321 tgccccgcgc gaacaatatg catgtaaaat ttgcgttgaa tataacgccc tgagcggcag
     1381 aaatgcttat ttaccgcgtt ttttcttttc cgtaccggca ggcaatccat tccgccgcaa
     1441 tgctcacggc aatctcttcc ggcgattccg cgccgatatc gagacccacg ggagaatgaa
     1501 tgcgcgcaat ctgttccttg gaaaacccgc gcgaaaggag ctggtcacgc atcttctttg
     1561 cgtgcgcatg actgccgatg gcgccaatgt accccgcgcg cgttcgaagc gccgcgtcga
     1621 ggatgaaaaa gtcgattccc ctgccgcgca ccatcgaaat gacggcgtcg cgttcggtga
     1681 gctccagttc gtcaagaaac ctttcctgca tttcgctcag cacccgttca gccataggaa
     1741 accgggaaga gtcgcagagc gtatccctgc cctccaccac ccagacgcgc aatccaagct
     1801 gcgccaaaac cggcacgagc gcttttgcca catgccctcc gccgagaata acggcgcgcg
     1861 gcggcggata gagcggttcg ataaaccgcc tcggttcctc cgtgccaagc accggcgcgg
     1921 cgcttaaaaa aggcttgagc gcgggatcgt cctttgccgc ccaggcggga acgacctccg
     1981 tttctcccac cgttccgtga tcgatggaac agaccagata cccttcctcg cggtcaagca
     2041 cttccagcgc gcgctcagcg agttttctcg cgctctcacc cgtaaataac cgcagcagaa
     2101 cgtcgatttc tccgccggaa acggcgcagc cctcgccttc gcctgggtgt atggcgtacg
     2161 agcgcagcgc ggagacgttt tcccgcagca gagcgagcgc gtcctgcttt acctgatact
     2221 ccgcttcacc gccgcccacc gttccagtct gcgcgccgtt ttcgtccacg accatcgcag
     2281 ttccaacgcc gcgcggcgtg gttcccctgc gggaaacgat cgtgcagagc atggcgcttc
     2341 tgccgctcgt gctggcggca agaatcgctt cgagcatcct cttcatccgg cgttccccct
     2401 ttatgaaacc tctgactgcc gttcaggctg tgcaaaagct gttctgtaaa aactcagttt
     2461 ttctttgcgc ccgcttcaaa gatcaaaaga cccagcttgc gcgttttggc aaagtagagc
     2521 ggcagagcgg gatcgtttgt tttagaaagc agcggctcca cttcctccgc ggaacattcc
     2581 ctgcgcgaat agaacgaaaa gaacgcgcag gcgtcttcta gcgaagcaaa cggctggccg
     2641 tgctccactt caaacagctc cagcgaatac gaaatgttct gctctttgag ctcgtttgca
     2701 atatcgtgta tg
//
LOCUS       DAOMGY010000163         2701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_41143_length_2701_cov_3.576342, whole genome shotgun sequence.
ACCESSION   DAOMGY010000163 DAOMGY010000000
VERSION     DAOMGY010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41143_length_2701_cov_3.576342"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1220)
                     /locus_tag="PLR57_03380"
     CDS             complement(222..1220)
                     /locus_tag="PLR57_03380"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HQQ40528.1"
                     /translation="MDYESIKQYIGNRQQLFSVRESCLSGGRADGVRVIDVQNGGNLS
                     VTIVPDRCMDIFSVRFKGRCLNYITSNGIAHPAYYEVTGEGWSEAFSGGFLYTCGLSN
                     TGLRSGTEWDAQKEHGCIANRPAERVNILELETPDGPAVEITGIMNEGMLAGTNLSLT
                     RSIRLYYQRDVIEVTDTVKNEGFRRVPHTILYHCNMGYPLLQPDSKFFIPHSAVRPRT
                     PFAEEMLPRLDEILPPQDALEEMCYYYSIVPDDSGWCAAVLENARENLRLRLRFDAST
                     LDHFVQWKNFVKGEYIMGLEPANAPIDGREDALVRNELPYLEGGETRTYRLIFELD"
     gene            complement(1264..>2701)
                     /locus_tag="PLR57_03385"
     CDS             complement(1264..>2701)
                     /locus_tag="PLR57_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HQQ40529.1"
                     /translation="GHANEEYLLLAEKPGYVELPAETYWQAVLSGAKSAIARAGIDPK
                     DIVSLSATTQSETFIPIGPDGTPLRNAIVWLDERADEQAKRLAQTFDAARFYRETGLP
                     ELSGYTPVSKLLWVKENEPELYEKTVKFLLLEDYILYRLTGKFVTEKALACSSGWFHF
                     SGDCYWQEILDAAGLDADKLPELLECGQIVPGGVLPEVRKLLGLSDSAAVVTGAMDQT
                     AGAVGAGNVVPGLVTETTGTALCVVATLDQPDLDHPARVTLYRHIERGKYLMITIAMS
                     AGMFLKWFKDNFCEPESEQAEREGKSVYALFDQIVEATEPGANGLIAYPYLNGSLQPH
                     NDPNARAIFFGAGLDSKKQHFLRAIFESIAFLLRENVELVEQVNRIEIKEIRSLGGGS
                     KSDIWRQIKADVTQRPIASLAESECTSLGAAILAAVALGYYPDAKTAAEQANRIVDWK
                     QPDASLAACYDALYQKYCALYPRLKDLF"
BASE COUNT          513 a          780 c          844 g          564 t
ORIGIN      
        1 cgtcctcgtg ccggacgatc tggagcgtta ccgtatacgg cgcggcggaa gaagccccaa
       61 acgtaaatac cggcagcgag cagagcagaa acaggatgtt gatgagcgca aggtcgccga
      121 cgcggccgac tgcgcgcatc agcggactgt ccgcgcgaaa gggagtttga gccatagttc
      181 tcctttgatc aggttcgctg gttaacggaa tttgagacgc ttcagtccag ttcgaagatc
      241 aggcggtagg tgcgcgtttc gccgccttcc agatacggca gttcgttgcg gacaagcgcg
      301 tcctcgcggc cgtcgatggg ggcgttggcg ggttccagac ccatgatgta ctcgcctttg
      361 acgaaattct tccactgcac gaaatggtcg agcgtggaag cgtcgaaccg gagccggaga
      421 cgcaggtttt cgcgcgcgtt ctccagaaca gcggcgcacc agccggaatc gtccggcacg
      481 atactgtaat aatagcacat ttcctccagc gcgtcctgcg gaggcagtat ctcgtccaga
      541 cggggcagca tctcttccgc aaagggcgtg cgcgggcgga cggcggagtg cggaatgaaa
      601 aatttcgagt ccggctgtaa aagggggtag cccatattgc agtggtaaag aatcgtatgc
      661 ggcacgcggc ggaagccttc gtttttgacc gtgtccgtca cttcaatcac gtcccgctga
      721 taatacaggc ggatgcttcg agtaagcgaa aggttcgttc ccgcaagcat gccttcattc
      781 attataccgg taatctccac ggcggggccg tccggcgttt ccagttcaag aatgttcacg
      841 cgctcggcgg ggcggttggc gatgcagccg tgctcctttt gcgcgtccca ctccgtgccg
      901 ctgcgaaggc cggtgttcga cagcccgcag gtatacagaa atccgccgga gaacgcttcg
      961 ctccagcctt cgccggtgac ttcgtagtag gcgggatggg caatgccgtt gcttgtgatg
     1021 tagttcaggc agcggccttt gaagcgcacg ctgaagatgt ccatgcagcg gtcgggcacg
     1081 atggtgacgg agaggttgcc gccgttttgc acgtcgataa cgcgcacgcc gtccgcgcgc
     1141 ccgccggaaa ggcatgattc gcgcacggaa aagagctgtt ggcggttgcc gatgtattgc
     1201 ttgatcgatt cgtaatccat attttctatc ctgttttcct tcaaataatg ttggctgcgc
     1261 tggttaaaac aggtccttta agcgcgggta gagcgcgcag tatttttgat acagtgcgtc
     1321 gtagcaagcg gcaagggacg cgtccggctg tttccagtcc acaatgcggt tggcctgttc
     1381 cgccgccgtc tttgcatccg gataataccc cagtgcaacc gccgcgagaa tggcggcgcc
     1441 gagggacgtg cattccgact ccgcgagcga ggcgatgggg cgctgggtga catccgcctt
     1501 gatctgccgc cagatatcgc tcttggagcc gccgccgagc gagcggattt ccttgatttc
     1561 gatgcggttg acctgctcga cgagctcgac gttttcccgc aggagaaacg cgatgctttc
     1621 gaagatggcg cgcagaaaat gctgcttttt cgagtccagc cccgcgccaa agaagatagc
     1681 gcgcgcgttg gggtcgttat gcggctgcag gctgccgttg aggtaggggt aggcgatcag
     1741 gccgttcgcg ccgggttcgg tcgcttccac gatttggtcg aacagcgcgt aaacggattt
     1801 gccctcccgc tctgcctgct cggattccgg ctcgcagaag ttatccttaa accacttgag
     1861 aaacataccc gccgacatcg cgatggtgat catcaggtac tttccgcgct cgatatggcg
     1921 gtacagcgtg acgcgcgcgg gatgatccag gtcgggctga tccagcgttg cgaccacgca
     1981 gagcgccgtg ccggttgttt ccgtaacgag cccgggcacg acgttgcccg cgccgaccgc
     2041 gcccgcggtc tgatccatag ctcccgtgac cacggcggcg gaatccgaaa gcccgagaag
     2101 cttccgcacc tccggcaata caccgccggg aacgatctgg ccgcactcga gcagctcagg
     2161 cagtttgtcc gcgtcgagac ccgccgcgtc gagaatctcc tgccagtagc agtcgccgga
     2221 aaaatggaac cagccggaac tgcaggcaag ggctttctcc gtaacgaact ttcccgtcaa
     2281 acggtagagg atatagtcct ccagcaggag gaacttgacg gttttttcgt agagttccgg
     2341 ttcattttcc ttgacccaga gcaacttgga aaccggcgtg tagccggaga gttccggaag
     2401 tcccgtttcg cggtagaacc gcgccgcatc gaacgtttgc gcgagacgct ttgcctgctc
     2461 gtctgcccgc tcgtccagcc agacgatggc gtttcgcagg ggagtgccgt ccggcccgat
     2521 ggggatgaag gtttcgctct gcgtggtggc ggagagcgaa acgatgtcct tcgggtcgat
     2581 tcccgcacgg gcaattgcgc tctttgcgcc ggaaagcacc gcctgccagt aggtttccgc
     2641 gggcagttcc acataccccg gcttttccgc cagcaaaagg tattcctcgt tggcgtggcc
     2701 g
//
LOCUS       DAOMGY010000164         2696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_41253_length_2696_cov_3.254449, whole genome shotgun sequence.
ACCESSION   DAOMGY010000164 DAOMGY010000000
VERSION     DAOMGY010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41253_length_2696_cov_3.254449"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLR57_03390"
     CDS             complement(<1..257)
                     /locus_tag="PLR57_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006822483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HQQ40530.1"
                     /translation="MFWHTIGLLFLGALIGWLASKIMHGKGGLIRNIIVGIAGSVLGG
                     WLADWIGIGTGNIWSIGGFLIAVGGACLLILEEAYLAGIDM"
     gene            complement(344..922)
                     /locus_tag="PLR57_03395"
     CDS             complement(344..922)
                     /locus_tag="PLR57_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQQ40531.1"
                     /translation="MEQTTQKPEELKPEHKENRAVTALKALGSAALGFGKAQLILLGV
                     NFVVVTTGMLIAGMKGWSPLIALGISLLDLLPVIGSGIVFVPWAIVALLLKNTSMAIT
                     VGATYIAMVVLRMILDPIITGKNIGLSPLLTLAASVAGLILFGAAGAIIGPAIAAVAN
                     ILFRVLFPKKSAKKAEQPEAGAGGSEQSPHGA"
     gene            complement(969..>2696)
                     /locus_tag="PLR57_03400"
     CDS             complement(969..>2696)
                     /locus_tag="PLR57_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40532.1"
                     /translation="GVRTLRKMFYFKLALSNVRRNRLTHLPYLIATAVMSGVFLLIAG
                     LLFSKSLTNTPSGNTASMLFAFGLVVYALFTFFFMMYINNFLIKRRKKEFGLYGILGL
                     DKRHIGRVLIWENLFVIGGGLLCGVAVALVFGRLLFLILIKLIHAAPGSSFYIAPIAY
                     LLMFGLFFVIFVVTNVINLVRIHTANPIALLSSEKRGEKDKKGLVPLAILGALLLGTA
                     YYFAWTADVPGFALGIFFPLAILVILATYLLFLCGSIVILRLLRLKKSLYYKPDHFVT
                     ISGMFHRMRQNSRGLATICILSTMLVVTISGTLSLYLGREEMTKGMYPYDVQVTVPEE
                     STKEQIAVYDGAIQRLAAEQNVVLSADKSKLITELPEGEEFRRNNFVWEDSEFVEAPT
                     LIFYDRSFRFDASGSETDCLAFVQAVSDYKDPAVGPDPIMHVSDFFTAREDGYGFYGG
                     LLFLGAFFAVLFLAVAVLILYFKQVTEGYEDKERFSILQQVGMDDHQVKKTINRQVLW
                     VFFLPLAATALHMLFASKIMAQMLRTFMLYDWSLVLCCIGGSLGAFALLYFGVYWLTA
                     RTYYRIVHR"
BASE COUNT          669 a          780 c          719 g          528 t
ORIGIN      
        1 ttcatgtcga tgccggccag ataggcttct tccagaatca gcaggcacgc gccgccgacg
       61 gcgatgagga acccgccgat cgaccagatg ttgccggtgc cgatgccaat ccagtctgca
      121 agccagccgc caagcacgct gcccgcgatg cctacgatga tattgcggat taatccgcct
      181 ttgccgtgca taatcttgct ggcaagccag ccgatcagcg cgccgagaaa cagcagtcca
      241 atggtatgcc agaacatgat agttttctcc cctttcgaag taatgcgtta ttaaacaatg
      301 ccaactgaac aaatctagtt cggtttacaa acaaaaaccg gtgtcacgcg ccatgcggac
      361 tctgttccga cccgccagcg cctgcttcgg gctgctccgc tttctttgcg ctcttctttg
      421 gaaacagcac ccgaaagagg atgttcgcca ccgccgcgat cgcgggaccg ataatcgcgc
      481 ccgcagcgcc gaacaaaatg agtccggcaa cggaagcggc cagcgtcagg agcggtgaaa
      541 gcccgatgtt cttgcccgta atgatcgggt caagaatcat ccgcagcacc accattgcga
      601 tatacgtcgc tcccacggtg atggccattg acgtgttctt gagcagcagc gctacgattg
      661 cccagggcac gaacacgatg ccggagccaa tgaccggcaa caggtcgagc aggctgatgc
      721 caagcgcaat cagcggcgac cagcccttca tgcccgcaat cagcatgccg gtcgtgacca
      781 caacgaagtt gaccccaagc aggatcagct gcgctttgcc aaagccgagc gctgcggagc
      841 caagcgcctt gagcgcggtt accgcacggt tttccttgtg ttccggtttc agttcttccg
      901 gtttttgcgt tgtttgttcc atatttcgca ttatatcgaa aagcgggtcg ccccgcaagg
      961 gaaccgcact acctgtgcac gattcgataa tacgtccgcg ctgtcagcca gtagacgcca
     1021 aagtacagca gcgcaaacgc gccgagcgat ccgccgatgc agcagagcac aagactccag
     1081 tcgtagagca tgaatgttct cagcatttgc gccatgatct tggaggcaaa cagcatgtgc
     1141 agcgcggttg ccgcaagcgg caggaagaac acccaaagca cctgacggtt gatggtcttt
     1201 ttgacctgat ggtcgtccat tcccacctgc tggagaattg agaagcgctc cttatcctcg
     1261 tagccttcgg tcacctgctt gaagtaaaga atcagcacag ccacggcgag gaacagcacc
     1321 gcaaagaacg cgccaaggaa caatagccca ccgtagaacc cgtaaccgtc ttcccgcgcg
     1381 gtaaagaagt cagaaacgtg catgatcgga tcgggcccga ccgcagggtc tttgtagtcg
     1441 ctgaccgcct gcacgaacgc aagacaatcc gtttccgaac ccgaagcgtc aaagcggaag
     1501 ctcctgtcat aaaagatcag cgtcggcgct tccacaaatt cgctgtcttc ccaaacgaag
     1561 ttattgcgcc gaaactcctc gccctccggc agttcggtga tgagcttgga cttgtctgcg
     1621 ctgagaacga cgttctgctc ggcggcaagc ctttggattg cgccgtcata tacggcaatc
     1681 tgttcctttg tggattcttc cggcacggtc acctgcacgt cgtaaggata catgcccttt
     1741 gtcatctcct cgcgtcccag atacagcgag agcgtgccgg agatagtcac caccagcatg
     1801 gttgaaagga tgcagatggt cgcaagtcct cttgagttct gccgcatgcg gtggaacata
     1861 ccggagatgg tcacaaaatg atccggcttg taatacaggc tctttttaag ccgcagcagg
     1921 cggagaatga cgatgctgcc gcataaaaac agcaggtacg ttgcgaggat aactaaaatg
     1981 gcaagcggga agaagatgcc gagcgcgaat ccgggaacat cggccgtcca ggcaaagtaa
     2041 tacgcggttc ccagcagcaa agcgccgaga attgccagcg gaaccaagcc ctttttgtcc
     2101 ttttctccgc gtttctcgct tgaaagcagc gcaatcgggt tcgccgtatg aatccgtacg
     2161 aggttgataa cattcgtaac cacaaagatg acaaagaaca gcccgaacat caaaagatac
     2221 gcaatcggcg cgatgtagaa cgaactgccc ggcgccgcgt gaatcagttt gatgaggatt
     2281 aagaacagca gccgcccgaa caccagtgct acggcaacgc cgcagagcag gccgccgccg
     2341 atgacgaaca ggttctccca aatcagcacg cgcccgatat ggcgcttgtc cagaccgaga
     2401 ataccgtaca gcccgaactc tttctttctg cgcttgatga gaaagttgtt gatgtacatc
     2461 ataaagaaaa acgtaaacag cgcgtacacc accagaccga aggcgaacag catggaggcg
     2521 gtgttgccgg acggcgtgtt ggtcaggctt ttggagaaca aaagccctgc gatcaacaga
     2581 aatacgccgc tcatcaccgc cgttgcgatt aagtacggca agtgcgtaag ccggttgcgg
     2641 cgaacgttgc tcagagcgag cttaaagtaa aacatcttac gcaatgtccg cacccc
//
LOCUS       DAOMGY010000165         2693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_41323_length_2693_cov_3.357089, whole genome shotgun sequence.
ACCESSION   DAOMGY010000165 DAOMGY010000000
VERSION     DAOMGY010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41323_length_2693_cov_3.357089"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="PLR57_03405"
     CDS             <1..1095
                     /locus_tag="PLR57_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40533.1"
                     /translation="LMSLAAAYPNVDFTWNVAFEGETYENDLTSLDLSGKTVTIEDLT
                     RALSGLPSLETVTFDDAAVFSTEDQLALVSAFPNVSFLWNVQLLDDLSVRSDVAELDL
                     RDYTVPDAAAFSDKLQLLPALTRLDMCNCGPTDEEMAAMRARYPGTKFIWMTKVSKWM
                     IRTDIQGFSTGNKRKFPDGMGYYIGKDFEYGNIHAEDFENLKYCTDLVALDVGHCDNI
                     GNIDFIAGLPKLKYLIISLCNLTDISALENQTDLEFLEMTYNYLTDISPIRNCTKLRF
                     LNLSANEVTSIDVYMGLTSLERLWINGNHLTEDQVAELQAALPNTLIKAHPTSNEYAL
                     SLWRKGNEGYLTIQAMFGLRAQHQGSVPAG"
     gene            1142..2053
                     /locus_tag="PLR57_03410"
     CDS             1142..2053
                     /locus_tag="PLR57_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HQQ40534.1"
                     /translation="MPQNAERHAYLITAHDKPEQLKLLLSLLDDSNNDLYLHIDKKAA
                     GIQNSDLTDAIKKAHIQFVPRLDARWGSEVFIDAIASLIREAVKTEHAYYHLLSGVDL
                     PLKPQQEIHAFFREHAGKEFVAFDQKSADQRVLVQRLGRYSFRRPVGPFALRAYKRFE
                     PLWNKLQSALGVNRIKRCPVVFQKGAVWFSITHAFALYVLEQFPHYRLYYRHSVCADE
                     IWLQTILVNSPFMERRSFSGWDDELAATMRYVDWSDGGRSPKVLVSEDYDALISSGAL
                     FARKFDQALDGAVIDRIAQFVRSRENQ"
     gene            2217..>2693
                     /locus_tag="PLR57_03415"
     CDS             2217..>2693
                     /locus_tag="PLR57_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40535.1"
                     /translation="MEVQPRPERRFLKAFIACRPAEKPLRFHWIFKVLFALSGAAATL
                     LFTQRGSTGIGFIGVFCSLAICALIAFRYQALERAFVRVSPAKLLVSAVFALYAAHRY
                     SEWYSASMSERILEKVGGVLHLSDIMCIFLERWLSVGIAAVALFALCTFFYVAIDRI"
BASE COUNT          568 a          801 c          710 g          614 t
ORIGIN      
        1 cttatgtcgc tcgctgcggc gtatccgaat gtcgatttca cctggaacgt tgcgtttgaa
       61 ggcgaaacgt atgaaaacga tctgacctcg ctcgaccttt ccggcaaaac ggttactatc
      121 gaggatctga cccgcgcgct ttcgggactg ccctcgctgg aaacggtaac gtttgacgat
      181 gccgccgtct tttcaacgga agatcagctt gcgcttgtca gcgcattccc caacgtttcg
      241 ttcctctgga acgtgcagtt gctcgacgac ctgtccgttc gttcggacgt tgccgaactg
      301 gatctgcgcg actataccgt gccggacgcg gcggcttttt ccgacaagct gcagctgctc
      361 cccgcgctca caaggctgga tatgtgcaac tgcgggccga cggacgagga gatggccgcc
      421 atgcgcgcgc gctatcccgg cacgaagttc atctggatga ccaaggtgag taagtggatg
      481 atccgcacgg acattcaggg cttcagcacc ggcaacaaac gcaagttccc cgacggcatg
      541 ggctactata tcggcaagga cttcgaatac ggcaacatcc acgcggagga ctttgaaaac
      601 ttgaaatact gcaccgatct tgttgcgctc gacgtcggcc actgcgacaa tatcggcaat
      661 atcgacttta tcgcgggctt gccgaaactg aagtacctga tcatttcgct ctgcaacctg
      721 accgatatct ccgcgctgga aaaccagacc gatctggaat ttctggagat gacttataac
      781 tacctgacgg atatttcgcc catccgcaac tgcacgaagc tccgcttcct caacctcagc
      841 gccaacgagg taacgagcat cgacgtctat atggggctga cgagtctgga acgactctgg
      901 atcaacggaa accatctgac agaggatcag gttgccgaac tgcaggccgc cctgcccaac
      961 accctcatca aagcgcaccc gacaagcaac gaatacgccc tgagcctctg gcgcaagggc
     1021 aacgaaggct atctgaccat tcaagcgatg ttcggtttgc gcgcgcagca tcagggttcc
     1081 gtaccggccg gttgataccg gttcaagcac gtaccgaaag acaaacgggg aggacgggtc
     1141 tatgccgcag aacgccgagc gccacgcgta cctgattacg gcacacgata agccggagca
     1201 gcttaagctg ctgctctcgc ttctcgacga ttcaaataac gacctctacc tccatatcga
     1261 caaaaaagcc gccggtattc aaaacagcga tctgacggac gcgataaaga aagcccacat
     1321 tcagttcgtt ccgcgtttgg atgcgcgctg gggcagcgaa gtgtttatcg acgcgattgc
     1381 ttcgcttata cgggaagcgg taaaaacgga gcacgcgtat taccacctgc tctccggcgt
     1441 cgatctgccg ctgaaaccgc agcaagagat tcacgctttt tttagagagc acgcgggcaa
     1501 ggagtttgtc gcgttcgacc agaagtccgc cgatcagcga gtgctcgtcc aacggctggg
     1561 gagatactcc ttccgtcggc ctgtcggccc gttcgccctc cgcgcttaca agcgtttcga
     1621 acccctttgg aacaaactgc aaagcgcgct cggcgtcaac cgcatcaagc gctgccccgt
     1681 cgtgtttcaa aaaggcgcag tgtggttttc cattacgcat gcgttcgctc tctatgtgct
     1741 ggagcagttc cctcattatc gtctctacta caggcactcc gtctgcgcgg acgaaatctg
     1801 gctccagacg attctcgtca actcgccgtt tatggagcgc cgctcttttt cgggttggga
     1861 cgacgaactc gccgccacca tgcggtacgt ggactggtcg gacggcggac gctcgccgaa
     1921 ggtgctcgta agcgaggatt atgacgcttt aatttcctcc ggcgcgctct ttgcgcgtaa
     1981 gttcgaccag gcgttggacg gcgcagtcat cgacaggatt gctcaattcg ttcgttcccg
     2041 cgaaaaccaa taaggcgcaa atcccgcatt tgcaaaaggg ttcttctctt aacagcgcat
     2101 gacctgcgct gttttcgtct tccggcaaag ccggtatcca accgtaagct atgcaattgt
     2161 gaaccgctgt tatgtatggt aaaattgatc caatcaaacg gaataaagga gcgacaatgg
     2221 aagttcaacc ccgccccgaa cgccggttcc tgaaagcctt tatcgcatgc cgtcctgccg
     2281 aaaagcccct gcgctttcac tggattttca aggttttgtt tgcgctttcc ggcgccgcgg
     2341 caacgctgct gtttacccag cgcggatcga ccggcatcgg ctttatcggc gttttctgtt
     2401 cgctcgcgat ttgcgctctt atcgcgtttc gttatcaggc gcttgaacgc gcgtttgtcc
     2461 gcgtcagtcc ggccaagctc ctcgtctccg ccgtgttcgc gctctatgcc gcgcaccgtt
     2521 actccgaatg gtacagcgct tccatgagcg aacgcattct ggagaaagtc ggcggcgtgc
     2581 ttcacctctc cgacatcatg tgcatttttc tagagcgctg gctctccgtc gggattgcag
     2641 ccgtcgctct ttttgcgctc tgcacattct tctacgttgc gatcgaccgt att
//
LOCUS       DAOMGY010000166         2680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_41617_length_2680_cov_2.878857, whole genome shotgun sequence.
ACCESSION   DAOMGY010000166 DAOMGY010000000
VERSION     DAOMGY010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41617_length_2680_cov_2.878857"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLR57_03420"
     CDS             <1..542
                     /locus_tag="PLR57_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQQ40536.1"
                     /translation="FLEGVQATDPEVKVDTRYVNSYVDTATAKEMGLSMINDKNCDII
                     WGVAGLSGNGAAEAALESGKAWFIGVDSDQELTLSSELAAITLTSGLKNVGNSLVWIF
                     DEWDAGRTYWGQQVNLGLAEGGVGVVTDKNFATVAPQAVQDKVLAAEAAIKDGTIVVP
                     SAIGDSTDAVKALRDSMLP"
     gene            829..2394
                     /locus_tag="PLR57_03425"
     CDS             829..2394
                     /locus_tag="PLR57_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40537.1"
                     /translation="MEQNELVVQMKGITKVYPNGIAANQSVDFSVRRGEIHALMGENG
                     AGKSTLMKMLFGLEQPTEGEIIVNGEKVSLTSPTVAIAHGIGMVHQHFMLVPSLTVAE
                     NIVLGMVLKKSGTFIDYKKVLDLTREYSERFNLAVNPDAKVMDIPVGMKQKVEILKAL
                     IRGAKILILDEPTAVLTTQETAELFKELVHLKEQGYTIIFISHKLNEIKQITDRLTIM
                     RGGKSMGVYETASISEQEISRLMVGRDVVLEVEKDTAKPTDVVLRVRGLRYTNEWNKK
                     MLDGISFDVRKGEILGIAGVEGNGQRELVDMLFDLEEPDEGVALVNGEDIIKASQSRI
                     RSLGVSLVPEDRMTYGIADAASIEENLISDRFEEKRFNHGPIFNVKAIRAESDRLIRD
                     YTVLCKSRRQKVKMLSGGNIQKVVVAREFSSNPTLIIADQPTRGIDVGATEFIRKRLV
                     DLSRAGAAVLLVSADLNEVMELSDSLIVLYGGKIAAYFEDTSQLDDMMMGEYMLGIKR
                     QSDEEIGRVCHEG"
     gene            2384..>2680
                     /locus_tag="PLR57_03430"
     CDS             2384..>2680
                     /locus_tag="PLR57_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40538.1"
                     /translation="MKDKRMFAFELVRGAAAILLALGVGTIFIFLSSDEPLTALRYLL
                     IGPLVSTKGGVASFNTQGLYTILAAMIPTILTGLAVCVMFSSNQFNLAGEGIVMV"
BASE COUNT          630 a          646 c          802 g          602 t
ORIGIN      
        1 gcttcctcga aggcgtacag gcgaccgatc cggaagtcaa ggtggatacc cgctatgtca
       61 acagctatgt ggataccgca acggccaagg aaatgggcct ttccatgatc aacgataaga
      121 actgcgacat tatctggggc gttgcgggcc tttccggcaa cggcgctgcg gaagccgcgc
      181 tggaatccgg caaagcttgg ttcattggcg ttgactccga ccaggaattg accctctcga
      241 gtgaacttgc ggcgatcacg ctgacctcgg gcctcaagaa tgttggcaac tcccttgtct
      301 ggatctttga cgagtgggat gccggccgta cgtactgggg acagcaggtg aaccttggtc
      361 ttgccgaagg cggcgttggc gtcgtaaccg acaagaactt tgccaccgtt gcgccgcagg
      421 ccgttcagga caaggtcctt gcagcggaag ccgctatcaa ggatggcacc attgtggttc
      481 cgagcgcgat tggcgacagc acggatgcgg tcaaggcgct gcgtgattcc atgctgccgt
      541 aaaaagcagc gaaacgaaag gcggttcttt gtggaacgct ttttgtaagc agatctgcaa
      601 gatcggggga gagcgagaag cgctcccccg gtttgtttac aaatcgtgtt gcgagttctt
      661 tgcagggaga atcttacctg gcaacgttgc aatcactcga tcttttcgat atatttagta
      721 gtataatttt ttccggctgt agcgactgct gcagcttcaa atctttgatg tttcgtcgca
      781 cgaacgcggc gatctaccaa ttctaacatc caaaggggaa tccactctat ggaacaaaac
      841 gaactcgttg ttcaaatgaa gggcattacc aaggtgtatc cgaatgggat tgccgcaaac
      901 caaagtgtcg atttctccgt gcgccgcggc gagatccatg cgttgatggg ggaaaacggc
      961 gcgggcaaga gcacgctgat gaagatgctc ttcggcctgg agcagccgac cgaaggcgag
     1021 atcattgtga acggcgagaa ggtgtccctt acttcgccga cggttgcgat tgcgcacggc
     1081 atcggcatgg tgcatcagca ttttatgctt gttccgagcc tgacggtagc cgaaaacatc
     1141 gtgctcggca tggtgctgaa aaaaagcgga acgtttatcg actataaaaa ggttctggat
     1201 ctgacgcgcg agtattcgga acgtttcaat ctggcggtca acccggatgc gaaagtgatg
     1261 gatatcccgg tcggcatgaa acagaaggtc gagattctca aagctctgat tcgcggcgcg
     1321 aaaattctga ttctcgacga accgaccgcg gttctgacca cgcaggaaac ggcggaactg
     1381 tttaaggagc tcgttcacct caaggaacag ggctacacaa tcatctttat atcgcacaag
     1441 ctgaatgaaa tcaagcagat taccgaccgg ctgacgatta tgcgcggcgg aaaatcgatg
     1501 ggggtttacg aaaccgcttc gatttccgag caggagattt cccgcctgat ggttggacgc
     1561 gacgtggttc tcgaggtgga aaaggatacg gcaaagccta cggacgttgt gctgcgcgta
     1621 cgggggctgc ggtacacgaa cgaatggaat aaaaaaatgc tggacggtat ttccttcgat
     1681 gtacgcaaag gggaaattct tggaattgcg ggtgtggaag ggaacggtca gcgtgaattg
     1741 gtcgatatgc tgttcgacct ggaggagccg gacgagggcg tggcgcttgt caacggcgag
     1801 gacatcataa aagcatcgca gagccgcatc cgttcgctgg gcgtgtcgct tgtgcccgaa
     1861 gaccgcatga cttacggaat tgcggatgcc gcttcgatag aggagaacct gatttccgac
     1921 cggttcgaag aaaaacggtt caaccatggc ccgattttca acgtaaaggc gatacgtgcc
     1981 gaaagcgatc gtctcatacg ggattatacc gttctctgca aatccagaag gcagaaagta
     2041 aagatgctct ccggcggtaa tatccagaaa gttgttgtag cgcgcgagtt ttcgagcaat
     2101 ccgacgttga ttattgcgga tcagcctacg cgcggcatcg acgtgggagc aacggagttt
     2161 attcggaaaa gactggtcga tctttcccgc gcaggcgcgg cggttctgct tgtgtcggcg
     2221 gatctcaacg aggtaatgga gctttcggac agccttatcg tattgtacgg cggaaagatc
     2281 gccgcctatt ttgaagatac gtcccagctg gacgacatga tgatgggaga atatatgctc
     2341 gggatcaaac gccagagcga cgaggagatc gggagggttt gtcatgaagg ataagcgaat
     2401 gttcgcattt gagctcgttc gcggcgccgc cgccattctg ctcgcgcttg gcgtcggaac
     2461 gattttcata tttctgagtt cggacgagcc gctgaccgcg cttcggtacc tgctgatcgg
     2521 tccgctcgtt tcgacaaagg gcggtgtggc gagtttcaac acgcagggac tctatacgat
     2581 actcgcggcc atgatcccga ccattctgac gggccttgcg gtctgcgtga tgttttcgag
     2641 caaccagttc aaccttgcgg gtgaaggcat cgtaatggta
//
LOCUS       DAOMGY010000167         2676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_41691_length_2676_cov_2.771843, whole genome shotgun sequence.
ACCESSION   DAOMGY010000167 DAOMGY010000000
VERSION     DAOMGY010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41691_length_2676_cov_2.771843"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLR57_03435"
     CDS             <1..272
                     /locus_tag="PLR57_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQQ40539.1"
                     /translation="KCSETEGYGWEKLKSAIAETWPDALISPYLMIAASDSRHYCAIS
                     DNVYRFSAMALTKEERGYIHAHNERIPLDKIVKTVQFYVRLMRSL"
     gene            complement(338..913)
                     /locus_tag="PLR57_03440"
     CDS             complement(338..913)
                     /locus_tag="PLR57_03440"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HQQ40540.1"
                     /translation="MKIYDISRELLKTPPYPGDEPPVLKAVHRMKDGEKYNLSRLETS
                     THAATHVDAPLHFIEKGKDIASIPADVFVGECVVLTVPKTSLDSMYFMKRPFAPRILL
                     HGKGELTESAIGYLYNQGLILLGTDCQSVGTYSDELSAHTALLGYGIVLLENLDLRGV
                     PDGRYTLSAAPLKIAGAEGAPCRAVLISNER"
     gene            complement(1288..2595)
                     /locus_tag="PLR57_03445"
     CDS             complement(1288..2595)
                     /locus_tag="PLR57_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40541.1"
                     /translation="MSISDILSTAFLNLWRRKMRAFLTVLGMVIGVASIVVMVSLGIG
                     IKQATIESFAGTGSLTTIRVNSYSWVDNGNGGGTSRQTELNKKAVTAMKKIDGVQSVM
                     PLVTTYGMLKSGRYAMDVSILGVTKEDAEEFGIELSEGEIPGGGGSNTYEMVMGAWTL
                     NNFYDPDNYRPAVDSKGNPRVTSSSRIQLTFDYRNIYQSQMDYSSGDNGDTRPLGSYY
                     KVKVTGVMSEENNDFSYYCLMDMDQLVKLAKANKDYTSFDSSKYDTVLVKCKEIDDVK
                     TVKAAIEDMGYGTYSLQDAVEMAEESTKNVQYLLGAIGGVALLVAAIGIMNTMMMSIF
                     ERTKEIGIIKVLGCRIDNIAGLFLAESAYIGLFGGALGLVLSFGVGALLNQLLVSSGL
                     RSIIPAYLAFGAVGFSIIVALAAGLYPALRAMKLSPLAAIRNE"
BASE COUNT          538 a          780 c          773 g          585 t
ORIGIN      
        1 gcaagtgctc cgaaacagag ggctacggct gggagaaact caaatccgcc attgccgaaa
       61 cctggccgga cgcgttgatt tcgccgtacc tgatgattgc ggcttccgat tcgcgccact
      121 actgcgcgat ttcggacaac gtctaccgct tctccgccat ggcgctcacc aaggaagagc
      181 gcggctatat ccacgcgcat aacgagcgga ttccgctgga caagatcgtc aagaccgtcc
      241 agttctatgt tcgcctgatg cgctccctgt aaagcaaacg aatcaaaaac gcgctccgca
      301 aggagcgcgt tttgtgtcta ttcgaatatt agcgccgtta tcgctcgttg gagatgagca
      361 ccgcgcggca gggcgcgcct tccgcgccgg caatcttgag cggggcggcg gagagcgtat
      421 accgcccgtc cggcacgccg cgcaggtcga ggttttcgag cagcacgatg ccgtagccaa
      481 gcagcgcagt atgcgccgaa agttcgtccg aataggtgcc tacggattgg cagtccgtgc
      541 cgagcaggat cagaccctgg ttataaaggt agccgatggc gctttcggtg agctcgccct
      601 tgccgtgcag taaaatgcgc ggcgcgaacg gccgcttcat aaagtacatg gagtccagcg
      661 acgttttcgg caccgtgagc acaacgcatt cgccaacgaa cacgtccgcg ggaattgaag
      721 cgatgtcctt gcctttttct ataaagtgaa gcggcgcgtc cacgtgcgtc gccgcgtggg
      781 tgctggtttc gaggcgcgag aggttatatt tttccccgtc cttcatgcgg tgaaccgcct
      841 tcaaaacagg cggttcgtcg ccgggatacg gcggcgtttt gagcagttcg cgggagatat
      901 cgtaaatttt cataagcttc agtccaatcc gatttttggg gaggcaaacc ccattatacg
      961 tgcccgcgcg taaaaacgca aatccctctg tagtcacgct gaaatgcggg tttccaaagg
     1021 gcgtgccctt tggcgggtgc gcgagggcag agccctcgga tttcatatat tggggctccg
     1081 ccccaaaccc cgcgcagggg tacacccctg cgaaccccgc gtgcgggttt ccaaagggcg
     1141 tgccctttgg cgggagcacg agggcagagc cctcggattc catatattgg ggctctgccc
     1201 caaaccctgc caaagggata catcccttta gaatcccatc tggctggaaa caaaagtacc
     1261 cgccggaagg cgggtacttg tttgcgttta ttcgtttcgt atggccgcca acggggaaag
     1321 cttcatggcg cgcagcgccg gatacaagcc tgcggcaagc gctacgatga tggagaagcc
     1381 aaccgcgccg aaggccagat atgcggggat gatgcttctc agtcccgaac tgaccagcag
     1441 ctgattgagc aaagcgccga ccccgaagct cagcaccagc ccaagcgctc cgccgaacag
     1501 cccgatatac gcgctctcgg caaggaacag ccccgcgatg ttgtcgatgc ggcagccgag
     1561 caccttgatg atgccgattt ccttggtgcg ctcgaagatg ctcatcatca tcgtgttcat
     1621 tattccgatg gcggcaacca gcagcgcaac tccgccgatg gcacccagca ggtactgaac
     1681 gttctttgtg ctctcctcgg ccatctccac cgcgtcctgc aggctgtagg tgccgtagcc
     1741 catgtcctca atggcggctt tcacggtttt gacgtcgtca atctccttgc acttgaccag
     1801 cacggtgtcg tacttggagc tgtcgaagct cgtgtaatcc ttgttcgcct tggcgagctt
     1861 gacgagctga tccatatcca tcaggcagta atacgaaaag tcgttgtttt cctcgctcat
     1921 gacgccggtg actttgacct tgtagtaact gcccagcggg cgcgtatctc cgttgtcgcc
     1981 ggaggagtag tccatctggc tttggtagat attgcggtag tcgaacgtca gctggatgcg
     2041 cgaggaactg gtcacgcgcg gattgccctt gctgtccacc gccgggcggt agttgtccgg
     2101 atcgtaaaag ttgttgagag tccaggcgcc catgaccatt tcgtaagtat tggaaccgcc
     2161 gccgccaggg atttcgcctt cgctgagctc gatgccgaac tcctcggcgt cttccttggt
     2221 tacgccgaga atggaaacgt ccatggcgta gcggccgctt ttgagcatgc cgtaggtcgt
     2281 aaccagcggc atgacgctct gcacgccgtc gatctttttc atggcggtga ccgctttttt
     2341 gttcagttcc gtctggcggg acgttccgcc gccgtttccg ttgtccaccc aggagtagga
     2401 attgacgcgg atggtggtca ggctgccggt tccggcaaag ctctcgatgg tcgcctgctt
     2461 gatgccgatg cccaaagaga ccataacgac gatggaagca acgccgatta ccatgccaag
     2521 aacggtcaaa aacgcccgca ttttgcgccg ccacaggttg agaaacgccg tggacagtat
     2581 atcgctgata ctcatttatc ctccaagtgc ttgagtccgt ccgccgctga cggcggagcg
     2641 tcagatatca tcatccttaa gcgagcgttc gcgttt
//
LOCUS       DAOMGY010000168         2675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_41711_length_2675_cov_3.649618, whole genome shotgun sequence.
ACCESSION   DAOMGY010000168 DAOMGY010000000
VERSION     DAOMGY010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41711_length_2675_cov_3.649618"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1412
                     /locus_tag="PLR57_03450"
     CDS             168..1412
                     /locus_tag="PLR57_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ40542.1"
                     /translation="MKKLLCMVMVIMMAFAVLTGCTAKTEPAATAADAAPAADATAAE
                     EPATAAETIKVGILGPHTGDYAVYGLAVKNGAQLYIDQVNAAGGVNGKQIETIVYDNK
                     GDSTEAITAFTRMVDEGITALIGDVLTGNTIAVVGEAYPINMPMITASATAAAVTYDA
                     DSDTVFTNVFRTCFIDPFQGEKMAQYAAEKLGATTAAIITQTGNDYSVGLADAFKAKC
                     AELGITIVAEEGYSEGDVDFKSQLTNILAAAPDVVFCPNYYQDDGMIVTQAREIGLTS
                     TFLGGDGWAGVAAYASAEDLEGSFYCSAYAPGSTDAVKAFEAAYTAAYGADTLNMFAA
                     TGYDAAMVLVAALAKAEESGAAPASDEYKQAILDGIKSEGPSVQGITSEAGYTFDEYN
                     NPIKSAVIMTVTGGAEVFQETF"
     gene            1537..2424
                     /locus_tag="PLR57_03455"
     CDS             1537..2424
                     /locus_tag="PLR57_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQQ40543.1"
                     /translation="MSAFSTFLRQVINGLQAGSIYALVALGYSMVYGIITLLNFAHGD
                     IIMIGAYIAWVSMAKFAMHPVFAIIAAILGCVLLGVSIEKAAYKPLRGSPRISLLITA
                     IGVSFLLENVAQMVFGANAQVIPSFFSIPTLMLGSIEVSGNAIVTVIVAAVSMILLTF
                     LVQRTKMGKAMRAVSEDTDAAQLMGININRTITFTFAVGSALAGIGSVLYCSAYPQAT
                     STMGAMLGLKAFVAAVLGGIGSIPGAMIGGFAIGLAEALVSAIGLSVWKDGVVFAILI
                     IVLLVKPTGFLGRPLQEKV"
     gene            2435..>2675
                     /locus_tag="PLR57_03460"
     CDS             2435..>2675
                     /locus_tag="PLR57_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40544.1"
                     /translation="MKQYKKIPMLLRYAINLLLFAALLAVGSLLIKDGVISKYYSKVL
                     MLIGINIILAVSLNISTGYLGQLPLASRVPQLAKVV"
BASE COUNT          550 a          827 c          743 g          555 t
ORIGIN      
        1 tctttgtggg agcgtgaact ccttaagtgt tgcacttagt ttgacaattc actatcaggt
       61 ttcaaacgcc ctgtacgcat ttgaaaagtc cgaacgattt taataccagg tttcaaacgc
      121 cctatatgca tttgaaaaga acaataataa actaggagga aaccacaatg aagaaattgc
      181 tctgcatggt catggtcatc atgatggcgt ttgccgtttt gaccggctgc acagcgaaaa
      241 ccgaacccgc agcaaccgct gccgacgcgg cacccgccgc cgacgcaacc gccgcggaag
      301 aacccgcaac cgccgccgaa actatcaagg tcggcatcct tgggccgcac accggcgatt
      361 acgccgtata cggcctggcg gtcaagaacg gcgcccagct ctacattgac caggtcaatg
      421 cggcaggcgg cgtaaacggc aagcagatcg aaaccattgt ttatgacaac aagggcgatt
      481 cgaccgaagc catcaccgcg tttacccgca tggtagacga aggcatcacg gcgctcatcg
      541 gcgacgtgct cacgggcaac accatcgcgg ttgtcggcga agcctacccg atcaacatgc
      601 cgatgatcac cgcttccgcg accgctgccg ccgtcaccta tgacgcggat tccgacacgg
      661 tgttcaccaa cgtcttccgc acctgcttca tcgatccgtt ccagggcgaa aagatggcgc
      721 agtacgccgc tgagaagctc ggcgcgacga ccgccgcgat catcacccag accggcaacg
      781 attactctgt cggccttgcg gacgcgttca aagccaagtg cgccgaactc ggcatcacca
      841 ttgttgccga agaaggctat tccgaaggcg atgtggactt caagtcccag ctcaccaaca
      901 ttctcgcggc cgctccggac gttgtgttct gcccgaacta ctaccaggat gacggcatga
      961 tcgtgaccca ggcccgtgaa atcggtctga cctccacgtt cctcggcggc gacggctggg
     1021 cgggtgttgc cgcttacgcc agcgcggaag acctcgaagg cagcttctac tgctccgctt
     1081 acgcccccgg ctcgaccgac gcggtcaaag cctttgaagc cgcttacacc gcagcctacg
     1141 gcgcggacac gctgaacatg ttcgcggcaa ccggttacga cgcggcgatg gttcttgttg
     1201 ccgctctggc caaggccgaa gaatccggcg cggcgcccgc cagcgacgaa tacaagcagg
     1261 cgatcctcga cggcatcaag agcgaaggcc cgtccgtcca gggtatcacg agcgaagccg
     1321 gctatacctt cgacgagtac aacaacccga tcaagagcgc ggtcatcatg accgtcaccg
     1381 gcggcgcgga agtcttccag gaaactttct aagccactgt acagtacaat ctgagtattg
     1441 gcacgtcccg cgccgcgcga aaatcgcggc gcgggacgaa gccgtgttaa agtaccctgc
     1501 ggcggctcgc cccgtcgtcc agagggaggg aaatcaatgt ccgccttttc cacgtttttg
     1561 cggcaggtca tcaacggcct gcaggcaggt tccatctatg ccctcgttgc gctgggctac
     1621 agcatggtgt atgggatcat cacgctgctg aattttgccc acggggacat cattatgatc
     1681 ggcgcgtata tcgcctgggt ttccatggca aagtttgcca tgcacccggt gtttgccatt
     1741 atcgccgcca ttctcggctg cgtgctcctt ggcgtatcga ttgaaaaagc cgcttacaag
     1801 ccgctgcgcg gttccccgcg catctcgctg ctcattaccg caatcggcgt ttcctttttg
     1861 cttgaaaacg tagcgcaaat ggtattcggc gccaacgcgc aggttattcc gagctttttc
     1921 agcattccga cgttgatgct tggttccatc gaagtttccg gcaacgcgat cgtaacggtt
     1981 atcgtcgccg ccgtttccat gattcttcta acgtttcttg tacagcgcac caaaatgggc
     2041 aaggccatgc gcgccgtttc ggaggatacc gacgcggctc agctcatggg catcaacatc
     2101 aaccgcacca tcacctttac gttcgccgtc ggcagcgcgc tggcgggaat cggttcggtg
     2161 ctgtactgct cggcctaccc gcaggcaacg tccaccatgg gcgccatgct gggtttaaag
     2221 gcgttcgtcg cggcggtgct cggcggcatc ggttccattc cgggcgcgat gatcggcggg
     2281 ttcgccatcg gccttgcgga agcgctggtt tccgcaatcg gcctctccgt ctggaaggac
     2341 ggcgtggtgt tcgccatttt gattatcgtt ctgctggtca agcccacggg attcctgggc
     2401 aggccgctgc aggagaaggt ttgagggcgc gcacatgaaa cagtataaaa aaatccccat
     2461 gctgctacgc tacgcgatca acctgctgct gtttgcggcg cttctcgctg tgggctcgct
     2521 gttgatcaag gacggcgtga tcagcaagta ctactccaaa gtgctcatgc tcatcggcat
     2581 caacatcatc ctcgcggtgt cgctgaatat ctccaccggc taccttggcc agctgccgct
     2641 cgcgagccgc gtgccgcagc tggccaaggt ggtct
//
LOCUS       DAOMGY010000169         2673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_41759_length_2673_cov_3.213522, whole genome shotgun sequence.
ACCESSION   DAOMGY010000169 DAOMGY010000000
VERSION     DAOMGY010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41759_length_2673_cov_3.213522"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /gene="ispE"
                     /locus_tag="PLR57_03465"
     CDS             <1..732
                     /gene="ispE"
                     /locus_tag="PLR57_03465"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQQ40545.1"
                     /translation="IMQSIDLCDTVSVERASDIVVTSTGMQLPYDNTVRRAAEQYRAL
                     TGRSAHIRVTKRIPAEAGMGGGSADAAATLLGLQKIYGEADEKTLFELGASIGADVPF
                     CMRGGVQRAEGIGEVLTPLRGMKLYFVVAKPKEGVSTKKLFSLLKLPRTMPDTTACIK
                     ALAVGDLHALAPLLFNALEEPAIELVPEIGAVKAALLEAGALAACMTGSGSAVFGLFE
                     TRELAETALPKLEKFAFSRVCESIG"
     gene            946..>2673
                     /locus_tag="PLR57_03470"
     CDS             946..>2673
                     /locus_tag="PLR57_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HQQ40546.1"
                     /translation="MRFTRFRKGTALLIALMMLALIVPTSSAPADTGVRFVRVLLSTE
                     SASNMNITVKGAYTLAQAQRSFQNGTLTVSASGSTVTVTHSTEGLLYSGSTATIERAV
                     LGRDAGYLTMRTAAGTRNYLGNFTFTASNGVLTVVNNVPLSQYLYGVVGYEMSNSFPL
                     EALKAQALAAKGYVLLKMKTTGSYDIGDTSSDQVYKGYDPSYTNVIAAVDATMGDVLY
                     YNNMPLYCYYAASNGGWMILPSDRWTSTLYDGAYSYGADPYDLANPSTPVECIYVPAN
                     YSETDMGTAAFAFLDARLMAMVAARGVIPQNFYFAGVQSIDSVVSSGRAGYADDLNHT
                     TVTITATVRANLNESLIPTPTPTFAPTPSPTPTFEPTPSPTPTFEPTATPTATPSPTP
                     EGQTPTPVPSDYTPTPSPSATLEPTATPSPTPTFAPTPSPTPTFAPTPSPTPAIETTR
                     TSQVSVTFTFADLMAAGFFSNTSLKITYATQTTNGWKLLHARYGHGVGMSQRGAQYMA
                     NIGKTYREILGFYYPGATMGTMGYTFPETVDSAQPQTAATSASTGLVKNGTVNLRAKA
                     STSASVVEQL"
BASE COUNT          576 a          823 c          765 g          509 t
ORIGIN      
        1 attatgcaaa gcatcgatct gtgcgacacg gtttcggtag aacgcgcaag cgatattgtc
       61 gtcaccagca ccggcatgca gctgccgtac gacaacaccg tgcgccgcgc ggcggagcag
      121 taccgcgcgc tgacgggaag aagcgcgcat atccgcgtta caaagcgcat tcccgcggag
      181 gcgggcatgg gcggcggcag cgcggacgcg gcggctacgc tcctgggact gcagaaaatc
      241 tacggtgagg cggacgaaaa aacgctgttt gagctcgggg cttctatcgg cgcggacgtg
      301 ccgttttgta tgcgcggcgg cgtgcagcgc gcggaaggga tcggcgaagt gctcaccccg
      361 cttcgaggga tgaagctcta tttcgttgtg gcgaaaccca aagaaggcgt ttccaccaaa
      421 aagctgtttt cactgctcaa gctgccgaga accatgccgg ataccaccgc ctgcatcaag
      481 gcgctcgccg tgggcgattt gcatgcgctt gcgccgctgc tgttcaacgc gctggaagaa
      541 cccgcgattg aactcgtgcc ggagatcggc gcggtcaaag cggcgctgct ggaagcgggc
      601 gctctggcag cctgcatgac gggcagcggg agcgcggtgt tcgggctgtt tgaaacgcgc
      661 gagcttgccg aaacggcgct gcctaagctg gagaaattcg ctttttcgcg tgtctgcgag
      721 agtattgggt aagtattgct tctttacaga agaaacagtt ttctgcttct ctttttcaaa
      781 agagaagaga aaaaagaaaa acccatggta gcggcgctgt gaacaatccg cgaatattca
      841 aaaaatagag tgccaagaca ggcaaaatcc gatacaatca cagcacgggg tccctgttgt
      901 tacatccccg tttcatcgca aatcgacttc gggggacata ccatcatgcg tttcacccgc
      961 ttccgaaaag ggactgcact cttaatcgcg ctcatgatgc tggcgttgat cgttcccacg
     1021 tcctccgcac ccgcggatac gggcgtgcgg tttgtgcgcg tgctgctctc tacggaatcc
     1081 gcctcgaaca tgaatattac ggtaaagggc gcgtatacgc tcgcgcaggc gcagcggtcg
     1141 ttccagaacg gaacgctgac ggtctccgct tccggcagca cggtaacggt gacgcattcc
     1201 acggaaggct tgctgtattc cggcagcacg gctaccattg aacgcgcggt tctcgggcgc
     1261 gacgcggggt atttaaccat gcgcaccgcg gcgggcacgc gcaactacct cggcaacttt
     1321 acgtttaccg ccagcaacgg cgttctcacc gttgtcaaca acgttccgct ctcgcaatac
     1381 ctctacggcg tggtcggcta cgaaatgagc aactctttcc cgctcgaagc gctcaaagcg
     1441 caagcgctgg ccgccaaggg atatgtgctg ctcaagatga aaaccaccgg cagctatgat
     1501 atcggcgaca cttcttccga tcaggtatat aagggttacg accccagcta caccaatgtc
     1561 atcgccgccg tggacgcgac catgggcgac gtattgtact ataacaatat gccgctctac
     1621 tgctactacg ccgcgagcaa cggcggctgg atgattttgc cttccgaccg ctggacttcc
     1681 acgctgtatg acggcgcata tagctacgga gccgatcctt acgatctggc gaatccttcc
     1741 acgccagtgg aatgcatcta cgtgcccgcc aactacagcg aaacggacat gggtacggcg
     1801 gcgtttgcct tcctcgacgc acgcctgatg gcaatggtcg cggcgcgcgg cgttattccg
     1861 cagaactttt actttgcggg cgtgcagtcg atcgacagcg tcgtttcctc cggcagagcg
     1921 ggctacgcgg atgatttgaa ccacacaacg gtaacgatta cggccacggt tcgcgccaac
     1981 ttgaacgagt cgctgattcc tacgccaacg cctacgttcg cgcctacgcc ttcgcccacg
     2041 ccaacgtttg aaccgacgcc ttcgcccacg ccgacgtttg agccgaccgc gacgccaacc
     2101 gcgacgccga gcccgacgcc ggaggggcag accccgacgc ctgtgccgag cgactatacg
     2161 ccaacgccgt ctccctcggc gacgctcgag cccacggcta cgccttcgcc cacgccgacg
     2221 tttgcgccaa caccttcgcc aacgcctacg ttcgcgccca caccttcgcc aacgcccgca
     2281 atcgagacca cgcgcacaag ccaggtttcc gtgacgttta cgtttgcgga tttgatggcg
     2341 gcgggcttct tttcgaacac cagtttgaag ataacctacg ccacccagac gaccaacggc
     2401 tggaagctgc tgcacgccag atacggccac ggcgttggca tgagtcagcg cggcgcgcag
     2461 tacatggcga acattgggaa aacctaccgc gaaatactgg gattctacta tcccggcgcg
     2521 accatgggaa ccatgggata cacgttccct gaaacagtgg acagcgcgca gccgcaaacc
     2581 gcggccacca gcgcttccac aggtctggtc aaaaatggaa cggtcaacct gcgtgcaaag
     2641 gcttccacga gcgcgagcgt agttgaacag ctt
//
LOCUS       DAOMGY010000170         2672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_41782_length_2672_cov_2.951471, whole genome shotgun sequence.
ACCESSION   DAOMGY010000170 DAOMGY010000000
VERSION     DAOMGY010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41782_length_2672_cov_2.951471"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLR57_03475"
     CDS             <1..199
                     /locus_tag="PLR57_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HQQ40547.1"
                     /translation="ASLTVHDFRVVPGHTHTNLVFDVVVPFGFPLSDEEVRTKISERV
                     LALEKNCYAVVQVDKASIAKE"
     gene            268..2034
                     /locus_tag="PLR57_03480"
     CDS             268..2034
                     /locus_tag="PLR57_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQQ40548.1"
                     /translation="MLTKWGKSLDPSNLLPEYPRPQFQRQSYYSLNGAWEFAFTPLGM
                     QTPPPRFDGTILVPFSPESELSGVKRTLKPNECLWYRRYVETPAGFDANQEDLILHFG
                     AVDQFAEVRVNGMSVVNHAGGYLPFSAIVTEFFPLGGVAEVVVRVRDETDTSCWSRGK
                     QSSKPGGIWYTPQSGIWQTVWMERVPKRRIESLRITPLFDEKAVELLVWTNCTGAGSI
                     RLGMSETPFTDGIPVKISLPEFTPWSPENPKLYDFTLSMERDRVESYFAMRKFGVGKD
                     AEGKPRLMLNNEPYFHSGVLDQGYWPDGLYTAPSDEAMVNDISLMKSMGFNMLRKHIK
                     IEPLRWYYHCDRLGMLVWQDMVNGGGLYGTFAIAAPLVLGNSHPDNDYAYFAREEVRG
                     REAYTRELRDTVRHLYNVCSLAMWVPFNEGWGQFDANRAVEEIRALDTTRTIDHASGW
                     HDQKGGDVKSLHVYYKPYRFTPDTLGRAVVLSEFGGYSQHIPEHSFCPKEFGYKRCKT
                     TEALVKDFERLYEREVIAAKDKGLAAAVYTQLSDVEQETNGFVTYDREMVKFDVARVR
                     AINEKLTGFASEPPKTPEQAKE"
     gene            2090..>2672
                     /locus_tag="PLR57_03485"
     CDS             2090..>2672
                     /locus_tag="PLR57_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ40549.1"
                     /translation="MEQELETRPAGEPAQPVTEALPSAEEKKRGFRNILLIGLTSMFM
                     DASTEMVYPLIPLYLVTAFGATPALVGVIEGIAESLASLMKVFSGYLGDRYKRKKALA
                     IGGYSTALIYKIALILSTSWVGVLTARVIDRFGKGVRVAPRDALVSENAAKTKQGTAF
                     GLHKALDMLGSAFGVLASYFILRAGAGAADYERV"
BASE COUNT          583 a          742 c          819 g          528 t
ORIGIN      
        1 cgcgagtttg acggtgcacg acttccgcgt cgtgccgggg cacacgcata cgaatcttgt
       61 gttcgacgtt gttgtgccgt ttgggttccc gctttcggac gaagaagtcc gcacgaagat
      121 cagcgagcgc gtacttgcgc tcgaaaagaa ctgctacgcc gtcgttcagg tggacaaggc
      181 cagcatcgcg aaagagtaag cgcaaaaaga ggggaagaag ttcggttcag tccgacagcg
      241 gcgccgaacg cacacaggag gaacgtcatg ctgacaaaat ggggcaaatc gctcgaccca
      301 agcaatcttc tgccggaata tccaagaccg cagtttcagc ggcagagtta ctactcgctc
      361 aacggcgcgt gggaattcgc gtttacccca ttggggatgc agacgccgcc gcctcgtttt
      421 gacggcacga ttctggtgcc gttctcgccg gaaagcgaac tttccggcgt caagcggacg
      481 ctaaagccca acgagtgcct ctggtaccgg cggtatgtgg aaacacccgc gggcttcgac
      541 gcgaatcagg aggacttaat tctccatttc ggagcggtgg atcagtttgc ggaagtgcgg
      601 gtcaacggca tgtccgttgt gaatcacgcc gggggctacc tgccgttctc cgcaatcgtg
      661 accgagttct ttccgctggg cggcgttgcc gaggtggtgg ttcgcgtgcg ggatgaaacg
      721 gacacctcct gctggtcgcg cggcaagcaa agcagcaagc ccggaggcat ttggtatacg
      781 cctcagagcg gcatctggca gacggtttgg atggagcgcg tgccgaaacg gcggattgag
      841 tcgctccgca ttacgccgct gttcgacgaa aaagccgttg agctgctcgt ttggaccaac
      901 tgcaccggcg cggggagcat tcggctcggc atgtcggaaa cgccgtttac ggacggtatc
      961 ccggttaaaa tttccctgcc ggagtttacg ccctggagcc cggaaaaccc caagctctat
     1021 gattttacgc tttcaatgga gcgcgaccgc gtcgagagct attttgccat gcgcaaattc
     1081 ggcgtcggca aggacgcgga aggcaagcca cggctgatgc tgaacaacga gccgtacttc
     1141 cacagcggcg tgctggatca gggatactgg ccggacggcc tctacaccgc gccttcggac
     1201 gaagcgatgg tcaacgatat ctcgctgatg aaatccatgg gcttcaacat gctccgaaaa
     1261 catatcaaaa tcgagccgct ccgctggtac tatcactgcg accggctcgg tatgctcgtt
     1321 tggcaggaca tggtcaacgg cggcgggctc tacggaacgt tcgccatcgc ggctccgctc
     1381 gtgctgggca actcgcatcc cgacaacgac tacgcctatt ttgcccgtga ggaagtgcgc
     1441 ggcagagagg cgtatacgcg cgaactgcgc gataccgtcc ggcacctcta caacgtctgc
     1501 agcctcgcca tgtgggttcc gttcaacgaa ggctgggggc agttcgacgc aaaccgcgcc
     1561 gttgaggaga ttcgcgcgct ggatacgacc cgcaccatcg accatgccag cggctggcac
     1621 gatcaaaaag gcggggatgt taaaagcctg cacgtgtact acaagccata ccgcttcacg
     1681 cccgacacgc tcggcagagc ggttgtgctc tcggaattcg gcggctacag ccagcatatt
     1741 ccggaacaca gtttctgccc caaagagttt ggctataagc gctgcaaaac gaccgaagcg
     1801 ctcgttaagg attttgaacg cctgtatgag cgcgaagtga ttgcggcaaa ggacaaaggg
     1861 cttgcggctg ccgtctatac gcagctttcg gacgtggagc aggagacgaa cggctttgtg
     1921 acctacgacc gcgagatggt caagttcgac gttgcccgcg ttcgcgcaat caacgaaaaa
     1981 ctgacgggat tcgcatcgga accgccgaaa acgccggaac aggcaaagga ataaccgttc
     2041 agcgaaattt tgagcatatt catcctgaaa caacggcgga ggtattatct tggaacagga
     2101 actggaaaca cggccggcag gcgaaccggc gcaacccgtt acggaagcgc tgccttccgc
     2161 ggaagagaaa aaacgcggat ttcggaatat tcttcttatc ggactgacca gcatgttcat
     2221 ggatgcgtcc acagagatgg tctatccgct gattccgctg tatctggtca cggcgtttgg
     2281 cgcaactccc gcgctggtcg gcgttatcga aggtattgcg gagagcctgg cgagcctgat
     2341 gaaagtcttt tcgggctatc ttggcgaccg gtacaagcgc aaaaaagcgc tcgccatcgg
     2401 gggctacagc acggcgctga tctacaaaat cgcgttgatt ctctccacct catgggtggg
     2461 cgtgttgact gcgcgggtaa tcgaccgctt tggcaagggc gtgcgcgtgg ctcccagaga
     2521 cgctctggtt tccgaaaacg cggcaaagac aaagcagggt acggcgttcg gactgcacaa
     2581 agcgctggat atgctgggca gcgcgttcgg cgttctcgct tcgtatttca ttctccgcgc
     2641 gggcgcgggt gcggcggatt atgagcgtgt gt
//
LOCUS       DAOMGY010000171         2666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_41905_length_2666_cov_3.774416, whole genome shotgun sequence.
ACCESSION   DAOMGY010000171 DAOMGY010000000
VERSION     DAOMGY010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41905_length_2666_cov_3.774416"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(354..1868)
                     /gene="lysS"
                     /locus_tag="PLR57_03490"
     CDS             complement(354..1868)
                     /gene="lysS"
                     /locus_tag="PLR57_03490"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQQ40550.1"
                     /translation="MEHPNTDQQVQEQELTQEQLSELLQIRRDKLTALQQAGNDPFFH
                     TVYNVSHTSEYILTHYEELEGKPVSVAGRMISRRIMGKASFVHILDGKGRIQAYVKLD
                     EVGEEAYAAFKTFDIGDIVGVKGTVFQTRTGETSIHATEITLLSKSLRPLPEKFHGLK
                     DPELRYRQRFVDLIVNPEVRSTFIKRSRIITEIRRRMDERGFIEVETPVLNTTASGAS
                     ARPFVTHHNTLDLDMYLRIATELHLKECVVGGLERVYEIGRLFRNEGMDPMHNPEFTT
                     IEAYQAYTDYNGMMELAEDLISRCCAAVNDTTKITYQGLAIDLTPPFRRLTMNESVRE
                     VTGADFYACATDEEARALAKSLKLEVKDKFATRGKLLAEAFDAFVEETLVQPTFIIDY
                     PVEISPLSKRKPSASYLTERFELFIAGHEFANAFSELNDPVDQRGRFVQQAQERANGD
                     DEAMMIDEDFCLSLEYGLPPTGGIGIGIDRLVMLLTDSASIRDVILFPTMKPVK"
     gene            complement(1941..2414)
                     /gene="greA"
                     /locus_tag="PLR57_03495"
     CDS             complement(1941..2414)
                     /gene="greA"
                     /locus_tag="PLR57_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQQ40551.1"
                     /translation="MAETWLTPEEIKKREERLEFLKTTRRLEIAEQLKIARAFGDLSE
                     NAEYDAAKNEQAKNEYDIVQLENELKNAHVIDESAIDTTTVGVGATVKLTDMSDKTKK
                     FTFQIVGSAETDPANGRISNESPIGKAVLGHKAGDVVEVVTPGGMLKLKVNSIGK"
BASE COUNT          485 a          777 c          731 g          673 t
ORIGIN      
        1 ttttcaaggt gccgtgcgct tccacgttcg ctttcgctcc cgtttccgct cgccgcccgc
       61 ttccgtttct ctcgcgggac agcttccaaa gtatagcaaa acagccatgg caaggcaaag
      121 cagactcgcg ttcgatatcc ggcataattc gttataaaac tccataattc gtttttattt
      181 caaaatgctt tgcttgtttg ttctcaaact ctcttattct ctcgttttcg ccaatcatgc
      241 cattaaactt ttgagctcat ttttttgccc tcaaaacgcg caaaaccgcc tcgtgtttga
      301 ggcggttttt tgctgaagca atcattgctg aaccttgctt gcctgatctg aatctacttc
      361 accggcttca tcgtcgggaa gaggatgacg tcccgaatgc tcgcggagtc ggttaaaagc
      421 atgacgagac ggtcgattcc gatgccgatg ccgcccgtcg ggggcaggcc gtattcgagc
      481 gagaggcaga agtcctcgtc gatcatcatg gcttcgtcgt cgccgtttgc gcgctcctgc
      541 gcctgctgga cgaaccgccc gcgctggtcg acggggtcgt tgagctccga gaacgcgttg
      601 gcgaactcgt gcccggcaat gaacagctca aaccgctcgg tcaggtacga cgcggagggc
      661 ttacgcttgc tcagcgggga gatctcgacc gggtagtcga tgataaatgt cggctgaacg
      721 agcgtttctt ccacaaacgc gtcgaacgcc tcggccagca gcttgccgcg cgtggcgaac
      781 ttgtccttga cttcgagctt gaggctcttt gccagtgcgc gcgcttcctc gtcggtcgcg
      841 caggcgtaga agtccgcgcc ggtaacttcc cgcacggatt cattcatggt caagcggcga
      901 aacggcggag tcaggtcaat tgccagtccc tgataggtga tcttcgtggt gtcgttgacg
      961 gcggcgcagc agcgggagat caaatcctcc gccagctcca tcatgccgtt gtagtccgta
     1021 tatgcctgat acgcctcgat ggtcgtaaat tcaggattgt gcatgggatc catgccttcg
     1081 ttgcggaaca gacgaccgat ctcgtacacg cgctccaaac cgccgaccac gcactccttt
     1141 aagtgcagct cagtagcgat gcgcaggtac atgtcgagat cgagcgtatt atggtgcgtc
     1201 acaaacggcc gtgcggaagc gccgctggcg gtggtgttga gcacgggagt ttccacctcg
     1261 atgaagccgc gctcgtccat tctgcggcga atctccgtaa taatccggct gcgcttgatg
     1321 aacgtcgagc gcacttcggg gttcacgatc aaatccacga accgctggcg gtagcgcagc
     1381 tccgggtcct tgagcccgtg gaacttctcc ggcagcgggc ggagggactt gcttaaaagc
     1441 gtaatttccg ttgcatggat gctggtttcg ccggttcgcg tctgaaacac cgttcccttg
     1501 acaccgacga tatcgccgat atcgaacgtc ttgaacgcgg cgtaggcttc ctcgcccact
     1561 tcgtcgagct tgacgtacgc ctgaatgcgc cccttgccgt caaggatatg cacaaagctc
     1621 gccttgccca tgatgcggcg ggaaatcatg cgccccgcga cggagacggg cttgccttcc
     1681 agttcttcat aatgtgtaag gatatactcg ctcgtatgcg acacgttgta aacggtatga
     1741 aagaagggat cgttccccgc ctgctgcagc gcggtgagct tgtcgcgccg aatctgcagc
     1801 agctcggaaa gctgctcctg cgttaattcc tgttcctgaa cttgttgatc cgtgttggga
     1861 tgttccatat tgcctctcct ctatcctgtg cttctgcaaa aaacaagggg gctcgccagc
     1921 ccccgaaaaa cctgtttgcg ttacttgccg atggaattga ccttcagctt cagcatgccg
     1981 ccgggggtga caacctcgac cacatcgccg gctttatgcc ccagcaccgc tttgccgatg
     2041 ggcgactcgt tggagatgcg cccgttggca ggatcggttt ccgcactgcc gacaatctgg
     2101 aacgtgaatt ttttcgtttt gtcgctcatg tccgtgagct ttaccgtagc gccgacaccg
     2161 acggtcgtcg tgtcgatagc ggactcgtcg atgacatgcg catttttcag ttcgttctcc
     2221 agttggacga tatcgtattc gtttttcgcc tgctcgtttt tcgccgcgtc atattccgcg
     2281 ttttcggaaa ggtctccgaa cgcgcgcgcg attttgagct gctccgcaat ctccagacga
     2341 cgggttgttt tgagaaactc caaacgctcc tcgcgttttt tgatttcttc cggcgtaagc
     2401 caagtttcag ccatcgttcg ttttctcctt atcgttccgc gcggacgcgt gcggcgcccg
     2461 ttcgtgttta cctattatat ttgtgcaaaa atagcatgtc aagcccggat tttccccgca
     2521 gtgcgcggtt ttttcgcgca gcgcgcccgg cggcgccgtt tacggcgcgt cgtccgcgcc
     2581 aaggccgtgt tccggccaga tttcctcaag ttccgtcagc gttttgcagg cgttgatgcg
     2641 cacgcggagc ttggaagcgc cccgtt
//
LOCUS       DAOMGY010000172         2663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_41976_length_2663_cov_2.942101, whole genome shotgun sequence.
ACCESSION   DAOMGY010000172 DAOMGY010000000
VERSION     DAOMGY010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41976_length_2663_cov_2.942101"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLR57_03500"
     CDS             complement(<1..307)
                     /locus_tag="PLR57_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQQ40552.1"
                     /translation="MKRKKRRVAIMGINPKRFDRSQIKFFAYLIPVTVIMGLPILFIV
                     FNAFKPLDELLTYPPKFITLRPTLENFRNLIATSTNKAIPMSRYLFNSVLSTALVVFF
                     "
     gene            complement(304..1224)
                     /locus_tag="PLR57_03505"
     CDS             complement(304..1224)
                     /locus_tag="PLR57_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQQ40553.1"
                     /translation="MKQFRTKLRAFNGRHSTFLLQLPFVVLFTLFVVVPVLVAIGLSF
                     TDFNSIQAPNFVGLRNYITILTDDSVFAANALPNTIRFAVIVGIGGYILSFLLAWSLA
                     QVPRMPRTIMAIILYLPSMTSGIMLSTVWQVVFAGDKVGYLNALLLRLEWIQKPILWL
                     SDSQYIMPIMILVSLWSCMGIGFLAMLAGILNINKDLYEAAYIDGVHNRFQEIIYVTI
                     PATRPYMMFGAVMSIVSAFQASSIGVLLTGGNPTPGYAGQLLVTHAEEHAFVRYEMGY
                     GAAVSVLVLLLVWGISQLAKRLFADKEGGK"
     gene            complement(1226..>2663)
                     /locus_tag="PLR57_03510"
     CDS             complement(1226..>2663)
                     /locus_tag="PLR57_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQQ40554.1"
                     /translation="NLSIDRIYLHAPGAKLPSPNAGFFSACSLSLKRFVYSFSTEMNE
                     SETESGDNAITVWINKPTQYVDALRELTAQEFTKQTGIAVTFSVMPDEKKITLANSTG
                     SNPDVALGLSYYRPAEFAMRGMAKNLLEYDDFVDWYGAEYNLSALNPMVYDQGIYGAS
                     ETQDFFVLFYRTDILDSLGLEVPQTWEDVKAMMPTLQRNAMSFNLPLANNVGYKTFEA
                     TSGFVFQNGGSFYSEDGLSAGFQNPNTIKALREMTDLYLVYGLSQNVPNFFNAFRSGS
                     VPIGVSNFATYLQLQQGAPELNGRWDIALIPGTLREDGVIDRSWQADATCAMIFNNTQ
                     KPDEAYAFFKWWLSSKTQLDYATTLRLKYGSDYIWNTGNLVALKGMPYSKAHLDVIAE
                     QWSWQKEVLRHPASYILEREVSNAWIAIVTKGEPFQPSVDQATIRCNSEIRRKLVEFG
                     YLDENGNALKIFNIHLVDELIAAQKGGQ"
BASE COUNT          609 a          727 c          769 g          558 t
ORIGIN      
        1 aaaagaacac gaccagcgcc gtggaaagca cgctgttgaa caggtagcgg ctcatcggaa
       61 tcgccttatt cgtggaggta gcgatcaggt ttcgaaagtt ctccagcgtt ggccggagcg
      121 tgatgaactt tggcgggtag gtcaagagct cgtcgagcgg cttgaacgcg ttgaacacga
      181 taaagaggat cggcaggccc atgatcaccg taaccggaat caggtaggcg aaaaacttga
      241 tctgcgagcg gtcgaaccgt ttcgggttga tgcccataat cgccacgcgg cgttttttac
      301 gcttcatttg ccgccctcct tatccgcaaa gagccgcttt gccagctgcg aaatgcccca
      361 aaccagcaga aggacgagca cggaaaccgc agctccgtaa cccatttcgt agcggacgaa
      421 cgcgtgctcc tcggcatggg tcaccaagag ctgacccgcg tagccgggcg tagggttgcc
      481 gccggtgagc agaacaccga ttgaacttgc ctgaaacgcg ctgacgatgg acatcaccgc
      541 gccgaacatc atgtacgggc gcgtcgcggg aatggtaacg tagataatct cctgaaagcg
      601 gttatgcacg ccgtcgatat acgccgcttc gtataaatcc ttgttgatgt tgagaatgcc
      661 cgcgagcatg gcgagaaagc cgatgcccat gcagctccag agcgaaacga ggatcattat
      721 cggcatgatg tactgcgaat ccgagagcca gagaatcggc ttttgaatcc actccagccg
      781 cagcagcagc gcattgagat atcctacttt gtcgccagcg aacaccacct gccagacggt
      841 ggagagcata attccgcttg tcatgctggg cagatagaga atgatcgcca taatcgtgcg
      901 cggcatgcgc ggcacctgcg ccagcgacca cgccagaagg aacgagagaa tatagccgcc
      961 gatgcctacg atgaccgcaa agcggatggt gttcggcagg gcgtttgccg caaacacaga
     1021 gtcatccgtc aaaatcgtaa tatagttgcg aaggccaacg aagttcggcg cctgaatcga
     1081 gttgaagtcc gtaaacgaca gcccgatggc caccagcacg ggaacgacta caaacagcgt
     1141 aaacagcacg acaaacggaa gctgcagcag gaaggtcgaa tgccgcccgt taaaggcgcg
     1201 cagtttcgtt cggaactgct tcatctcact gccctccctt ttgcgctgcg atgagttcgt
     1261 cgactaagtg gatattgaaa atcttgagcg cgttgccgtt ttcgtcgaga tacccaaact
     1321 cgaccagctt gcggcgaatt tcgctgttgc agcgaatcgt cgcctgatcg acgctcggct
     1381 ggaacggctc gccttttgta acgatggcaa tccaggcgtt gctcacctcg cgttcgagaa
     1441 tgtagctcgc gggatggcgc agaacttcct tctgccagct ccactgctcg gcgattacgt
     1501 cgagatgcgc cttggaatac ggcatcccct tgagtgcaac gaggttgccc gtgttccaga
     1561 tgtaatccga accgtattta aggcgcagag tggttgcgta atccaactgc gtttttgaag
     1621 agagccacca cttaaagaac gcgtaggcct cgtccggctt ttgcgtgttg ttgaaaatca
     1681 tggcgcaggt ggcgtccgcc tgccagctgc gatcaatcac gccgtcttcg cgcagcgtgc
     1741 cgggtatgag cgcaatatcc caccgtccgt tgagttccgg cgcgccctgc tgcagctgca
     1801 ggtaggttgc gaagttggaa acgccgatcg gcaccgagcc ggagcggaag gcgttaaaga
     1861 aattgggaac attttgcgaa agtccgtaca caaggtagag atccgtcatt tcacggaggg
     1921 ctttgatcgt attcgggttt tggaaacccg cggaaagccc gtcctcggaa tagaagctgc
     1981 cgccgttttg aaacacgaat ccggatgtcg cttcaaacgt cttgtagcca acgttgttgg
     2041 caagcggcag gttaaagctc atggcgttgc gctggagggt cggcatcatc gccttgacgt
     2101 cttcccaggt ctgcggaact tccagcccga gggaatcgag gatatccgtg cggtagaaca
     2161 gcacaaagaa atcctgcgtc tcgctcgcgc cgtaaatccc ctggtcgtac accatggggt
     2221 tcagcgcgct gaggttgtat tccgcgccgt accagtccac aaagtcgtcg tattccaaca
     2281 ggtttttggc catgccgcgc attgcaaact cagcagggcg ataataagaa agtccgagcg
     2341 cgacgtccgg attgctgccc gtcgagttgg cgagcgtaat ctttttttcg tccggcatca
     2401 cggaaaacgt aaccgcaatc cccgtctgct ttgtaaactc ctgcgcggtc agttcgcgca
     2461 gcgcgtccac gtactgcgtg ggcttattga tccagacggt aatcgcgttg tcgccgctct
     2521 ccgtttcgct ttcgttcatc tcggtggaga acgaatagac aaaccgtttg agcgatagcg
     2581 aacacgcgct gaaaaagccg gcgttgggag aaggcagctt tgcgccgggc gcatgcaggt
     2641 agatgcggtc gatgctcagg ttc
//
LOCUS       DAOMGY010000173         2654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_42166_length_2654_cov_3.412851, whole genome shotgun sequence.
ACCESSION   DAOMGY010000173 DAOMGY010000000
VERSION     DAOMGY010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42166_length_2654_cov_3.412851"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLR57_03515"
     CDS             complement(<1..445)
                     /locus_tag="PLR57_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40555.1"
                     /translation="MSRFGSSSQRHAGYRTTMLGKLGFNFYDVFVYAFCILFAILCIY
                     PMWYVLISSITPYEEYVKGGLMLLPLGGIDLQYYSAIFHTKAFTNSMWISVSKTVLAT
                     TLSMLITSTMAYACSKSHVKGMKLINALVVFVLFFTGGLIPQYMLY"
     gene            complement(461..1465)
                     /locus_tag="PLR57_03520"
     CDS             complement(461..1465)
                     /locus_tag="PLR57_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQQ40556.1"
                     /translation="MNRKGSPAPEKNGKASVPSKPYNSSFFTRMIRYIRKYWFLYMLV
                     LPGLCFLLVFEYAPMYGITLAFKDYSPRLGVLGSPWIGLDHFKEMFADPSFIRAFKNT
                     IIINVYNLVFGFTFSVFLALMINEIRLKRVKSVVQTAVYLPYFLSWVIFAGLVQVFLE
                     YPSSADIGGVVNQVITKLGGAPIDFLKRPDLFRGILVISNIIKTSGYSTIVYLAALAG
                     ISPTLYESAAIDGANRKDMLFHITIPRIMPSIAIMLILQLAGLFVSNFDQVYNLYNNY
                     VLSTGDVLSTYIYRISLGGGGTNFELSTATNFLLNAMGLVVVLIANKFVNKLDVQGIL
                     "
     gene            1794..>2654
                     /locus_tag="PLR57_03525"
     CDS             1794..>2654
                     /locus_tag="PLR57_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQQ40557.1"
                     /translation="MTKSIGFIGYGLRSRTMMKAFAGIDADIRVAAISDPRADEIQKE
                     VANDARFAGVQYEESAEALLKRNDLDGVFIGTRCSLHADLAAKVLKSGIPLYLEKPVC
                     INEAQYDRLKAASAGKTGRAVVSFPLRVSPLVREMKRIVESGLLGKLTMVQAVNNVPY
                     GSVYYHSWYRDPSETGGLFLQKATHDVDYIAYLTGETPKEVFARTTKLYYKGDKPAGL
                     RCPDCAEYKTCRESSYVVEHILKEEVTGDACCFAVDTGNEDGASAIMTYESGMIASYS
                     QNFVVKKGAAR"
BASE COUNT          675 a          652 c          781 g          546 t
ORIGIN      
        1 tatacagcat gtactgcgga atcaggccgc cggtaaagaa gagcacgaac acgacgagcg
       61 cgttgatgag cttcatgccc ttaacgtgcg acttactgca ggcgtaggcc atcgtggagg
      121 taatcagcat gctcagcgtg gttgccagaa cggtcttgga aaccgaaatc cacatgctgt
      181 tggtgaacgc tttcgtgtgg aagatcgccg agtagtactg caggtcgatg ccgcccagcg
      241 gaagcagcat cagtccgccc ttgacgtatt cttcatacgg cgtaatggag ctgatgagca
      301 cgtaccacat cgggtaaatg cagagaatgg caaagagaat gcagaatgcg tagacgaaca
      361 cgtcgtaaaa gttgaatccc agtttgccga gcatcgtggt tcgatagccc gcgtgccgct
      421 gagaggaaga accaaatcga ctcatactgc ttctcctttc ttacagaatg ccctgtacgt
      481 cgagcttgtt gacaaacttg ttggcaatga gcacgaccac gaggcccatg gcgttgagca
      541 ggaagttcgt tgcggtggaa agttcaaagt ttgtgcctcc gccgccaagg ctgatgcggt
      601 agatataggt ggagagcacg tcgcccgttg agagcacgta gttgttgtag aggttgtaaa
      661 cctggtcgaa gttggaaacg aacaagcccg ccagctgcag aatcagcatg atggcgatgg
      721 agggcatgat gcgcggaatc gtaatatgaa agagcatgtc cttgcggttc gcgccgtcga
      781 tggccgcgct ctcgtacagc gtcgggctga tgcccgcgag cgccgcgagg tagacgatgg
      841 tggagtagcc ggaggtctta atgatgttgg aaatcacgag gattccgcgg aacaggtccg
      901 gccgtttgag aaaatcgata ggcgctccgc cgagctttgt aataacctga ttgaccacac
      961 cgccgatatc cgcagacgac gggtactcca gaaacacctg cacaagaccc gcaaagatga
     1021 cccacgagag aaagtacggc aggtatacgg cggtctggac gacgctcttg acgcgcttga
     1081 gccggatttc gttgatcata agcgcgagga acacggaaaa cgtaaatccg aacacgaggt
     1141 tgtagacgtt gatgatgatc gtgttcttga acgcgcggat gaagctcggg tcggcgaaca
     1201 tttccttgaa gtgatccaga ccgatccagg ggctgcccag cacgccgagg cgcggggaat
     1261 aatccttgaa cgcaagtgta atgccgtaca tcggcgcgta ttcaaaaacc aaaaggaaac
     1321 acaaacccgg aagcaccagc atgtacagaa accagtactt gcggatgtac cgaatcatgc
     1381 gggtgaaaaa cgaactgtta tatggtttgc tgggaacgct tgccttgccg ttcttttccg
     1441 gcgcgggcga gcctttgcgg ttcatcgcgt ggccccctat tatcgtttat tagttaatat
     1501 tagctatcga agatttcggt gtgtttgcgc gattctttct ccggtcagga aatgaattcg
     1561 ctcgaattgg gcttaaaagc gtgttgttcc tacgatttcg gagctgcttt ctacgagcat
     1621 gatactaaat aaaacccgat aagtcaatat aagttaaaaa atagtagtta actattattc
     1681 aatttatggt agaataataa cgaaattagc aactaataat attgaaacgg ataaaatcgc
     1741 ttcgccggct agtcgggtcg gcagggcggc ggacggaaag ggaaccaatc ggcatgacaa
     1801 agagcatcgg ctttatcggt tacgggttgc gctcgcgcac gatgatgaag gcgtttgcgg
     1861 ggattgacgc ggatatccgc gttgcggcaa tcagcgaccc gcgcgcggac gaaattcaaa
     1921 aggaagtcgc aaacgacgcg cgctttgccg gagtacaata tgaagaaagc gccgaagcgc
     1981 tgctgaagcg gaacgatctg gacggcgtgt tcatcggcac gcgctgctcg ctgcacgcgg
     2041 atcttgcggc aaaggtgcta aaaagcggca taccgctcta tctcgaaaaa cccgtgtgca
     2101 tcaacgaggc gcagtacgac cggctcaaag ccgcctccgc gggaaagaca ggccgcgcag
     2161 tcgtgtcgtt tccgctgcgc gtttccccgc tggtgcgcga gatgaagcgc attgtcgaga
     2221 gcggcctgct tggcaagctc acgatggtgc aggcggtcaa caacgtgccc tacggcagcg
     2281 tgtattatca cagctggtac cgcgacccgt ccgaaactgg cgggcttttc ctgcaaaagg
     2341 cgacgcacga cgtggactat attgcctact taacgggaga aacgcccaaa gaggtattcg
     2401 cgcggacgac aaagctttat tataaaggag acaagcccgc gggactccgc tgcccggact
     2461 gcgcggaata caaaacctgc cgtgagtcga gctatgtggt ggagcatatt ctcaaggaag
     2521 aagtaaccgg cgatgcctgc tgctttgccg tggataccgg caacgaggac ggcgcgagcg
     2581 cgattatgac ctacgaaagc ggcatgatcg cttcctacag ccagaacttc gtggtcaaga
     2641 agggcgcggc acgg
//
LOCUS       DAOMGY010000174         2654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_42175_length_2654_cov_2.682570, whole genome shotgun sequence.
ACCESSION   DAOMGY010000174 DAOMGY010000000
VERSION     DAOMGY010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42175_length_2654_cov_2.682570"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLR57_03530"
     CDS             complement(<1..663)
                     /locus_tag="PLR57_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40558.1"
                     /translation="MEHIIEVSGLQKSYGSVHAVKGLDFYVESGKIFAFLGPNGAGKS
                     TTIDIICTFLKADAGTVVVDGSTLGKDDNAIRASIGAVFQDGLLDPLLTVQENLRIRA
                     GFYGLKGKKREKVIADVAKKAGVEDYLKRPYGKLSGGQRRRCDIARALVNTPKILFLD
                     EPTTGLDPQTRKSVWETITTLQHETGMTIFLTTHYMEEAAEADYVIVIDNGNIVAKGT
                     PSD"
     gene            869..1495
                     /locus_tag="PLR57_03535"
     CDS             869..1495
                     /locus_tag="PLR57_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQQ40559.1"
                     /translation="MPKTFSESEREYIKERLIDEAKKSLAQHGIRKTTVDELIKRVNI
                     PKGTFYLFYESKERLLFDVILRFNDEVQEQLLAEVTALPDVPDAQTLTDVIHRLYKQL
                     DDSFLPRLMLDGELEFFMRTLPPELASLHAEHDADKVRALIDLLPGVRPERAELYSAA
                     LRSVFLTLMYKDEIGEALFDDTIRMLIYGVVRQLVEESQAGGSAGQTK"
     gene            1532..2377
                     /locus_tag="PLR57_03540"
     CDS             1532..2377
                     /locus_tag="PLR57_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40560.1"
                     /translation="MIQVQQLQFSYTKQPFIEHVDFSVGSGEIFGFLGPSGAGKSTLQ
                     KILTGLIPRYGGSVVVDGEEVKSHGPRFFEKIGVDFEFPSLYEKLTARENLRFFASLY
                     PKHREIDALLESVELLRDADKKVGDYSKGMKSRLNFIKALLHDPQILFLDEPTSGLDP
                     SNARVMKDLILAEQAKGKTILLTTHNMYDATELCERVAFIVDGGLRALDSPRNLIMRR
                     GAAKVTYTYLEDGSERSAETPLAQTAQDETLQKLLRENRLRSIHSGEPTLNDIFVELT
                     GRQLQ"
     gene            2374..>2654
                     /locus_tag="PLR57_03545"
     CDS             2374..>2654
                     /locus_tag="PLR57_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40561.1"
                     /translation="MKTLALIRGDIRFQYKYGFYLLYLLFSLLYIGLLFAFPAAWREK
                     AAALMIFTDPAAMGLFFMGAIVLFEKNERVLDSLAVSPVKPWQYVATWGV"
BASE COUNT          569 a          751 c          786 g          548 t
ORIGIN      
        1 gtcgctcggc gtacccttgg caacgatatt gccgttgtcg atgacgatta cgtaatccgc
       61 ttcggcggct tcctccatgt agtgcgtggt gaggaagatc gtcatgcccg tctcgtgctg
      121 gagcgtggtg atggtttccc aaacgctctt gcgcgtctgc gggtcgaggc cggtggtcgg
      181 ctcgtcgaga aacagaattt tcggcgtgtt gaccagcgcg cgcgcgatat cgcacctgcg
      241 gcgctgaccg ccggagagct tgccgtaggg gcgcttgagg tagtcctcga cgcccgcttt
      301 tttcgcaacg tccgcaatta ccttttcgcg ctttttgccc ttcagcccgt agaaccccgc
      361 gcggatacgg aggttttcct gcacggtgag cagcgggtca agcaggccgt cctgaaacac
      421 cgcgccgatg ctcgcgcgaa tggcgttgtc gtccttgccc agcgtgctgc cgtccacaac
      481 gaccgtaccc gcgtccgcct tgaggaacgt gcagatgatg tcgatggtcg tggacttgcc
      541 cgcgccgttg gggccgagga aggcgaagat tttcccgctt tccacgtaaa aatccagccc
      601 cttgaccgca tggacggaac catacgattt ctgcaggccg gacacttcga tgatgtgctc
      661 caaaaagcag accccctcgt aaaaaactat gttcgaatca taacatactt catcaggtga
      721 ggcaaggggc tgtctggcga aaaatttcaa cctttccgaa gtgaaacacg agataatata
      781 ttgccgtgaa cttcttgaca agcagcgtac tttgtttcat aatgaccaaa acggcgaata
      841 tagtcaaata gaacgaaagg tgagcgaaat gcccaagacg ttctccgaaa gcgagaggga
      901 atacattaag gagcggctga tcgacgaggc gaaaaagagc cttgcgcagc acggcatccg
      961 caagaccact gtggacgaac tgatcaagcg cgtcaacatc ccgaagggga cgttctacct
     1021 cttttacgag tccaaggagc ggctgctgtt cgacgtgatt ctgcgtttca acgacgaggt
     1081 gcaggagcag ctccttgccg aagtgaccgc cttgccggat gtgccggacg cgcaaacgct
     1141 aacggacgta atccaccggc tttataagca gctggacgat tcctttctgc cgcggctcat
     1201 gctggacggc gagctggagt tctttatgcg tacgctcccg ccggagctcg cgagcctgca
     1261 tgccgagcac gacgcggaca aagtgcgcgc gctgatcgac cttttgccgg gcgttcgtcc
     1321 ggagcgggcg gagctctaca gcgcggcgct caggagcgtg tttttaacgc tgatgtacaa
     1381 ggacgaaatc ggcgaagcgc tgtttgacga tacgatacgg atgctgatct atggagttgt
     1441 gcgtcagctc gtggaagaat ctcaggcggg cggaagcgcc ggacaaacga aataaccgct
     1501 gttaccagcg agaaggatat tggaacaaac gatgattcaa gtacaacaac tgcagttcag
     1561 ctacacgaaa cagccgttta tcgagcacgt ggacttttcc gtcggcagcg gggaaatctt
     1621 cggctttctg gggccttccg gcgcgggcaa gagcacgctg cagaaaatcc tcaccgggct
     1681 gattccgcgc tacggcggca gcgtcgttgt ggacggcgag gaagtaaaat ctcacgggcc
     1741 gcgctttttt gaaaagatcg gcgtggattt tgagttcccc agcctctacg aaaagctgac
     1801 cgcgcgcgaa aacctgcgct tctttgcttc gctctatccc aaacaccggg agatcgacgc
     1861 cctgctcgaa agcgtggagc tcctgcggga cgcggacaaa aaggttggcg actactccaa
     1921 gggcatgaag tcgcgcctga actttatcaa ggcgctgctg cacgatccgc agattctgtt
     1981 tctggacgag ccgacctcag ggctcgaccc ttccaacgcg cgcgtgatga aggatctcat
     2041 cctcgccgag caggccaagg gcaaaacgat tctgctcacc acgcataaca tgtatgacgc
     2101 aacggaactc tgcgagcgcg tggcgttcat cgtggacgga ggcttacgcg cgctggacag
     2161 cccccgcaac ctcatcatgc ggcgcggcgc ggcaaaggta acctacacct atctggagga
     2221 cggttcggag cgttcggcgg aaacgccgct tgcacagacc gcgcaggatg aaacgctgca
     2281 aaagctcctc cgcgaaaacc gcctgcgctc catccattcc ggcgagccga cgctcaacga
     2341 tatcttcgtg gagctcacgg ggaggcagct gcaatgaaaa cgctcgcgct gatccgcggc
     2401 gatatccgct ttcagtataa atacgggttt tacctgctgt atctgctgtt ttcgctgctc
     2461 tatatcgggc tgctgtttgc gtttcccgcc gcgtggcgcg aaaaggcggc ggcgctgatg
     2521 atctttaccg acccggcggc gatgggactg ttcttcatgg gcgcgatcgt gctgttcgaa
     2581 aaaaacgagc gcgtgctgga cagccttgcg gtttcgcccg tgaagccctg gcagtatgtg
     2641 gccacctggg gcgt
//
LOCUS       DAOMGY010000175         2647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_42317_length_2647_cov_3.267361, whole genome shotgun sequence.
ACCESSION   DAOMGY010000175 DAOMGY010000000
VERSION     DAOMGY010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42317_length_2647_cov_3.267361"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PLR57_03550"
     CDS             <1..1044
                     /locus_tag="PLR57_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQQ40562.1"
                     /translation="SRRIAAAALCILFTLPLFLACSPAAEPAVATPEPTQATATPAPT
                     ATPVPSPTPFPEPTPFTVAWMTDTQEYAANHNDIFCAMTQWIADTQKEYNTVLTVHTG
                     DMIRGSYREYQWDNMVHAFSLLPKDMQILTVGGNHDIASFMPDYTPYLAYRPDTDTDA
                     EHSIFDGNVYYIEFEAGDVSILVFSITYGYEVESQDWINAVCAEHSNSYAILCLHSYL
                     EPAGYSSGGRYLFDGVVKKSPNVRLVLCGHEPGVRYQPEELDDDGDGVADRTVQQMLF
                     NMQEETKDGEGFLRLLQFDTVNDTINVITYSPHLDVYGYDWPGGDHFGGEHLIENAGI
                     AQYRIHSDTTPVN"
     gene            1111..>2647
                     /locus_tag="PLR57_03555"
     CDS             1111..>2647
                     /locus_tag="PLR57_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HQQ40563.1"
                     /translation="MSPTNRPGGRTKRTVSGGGNAYRRGSGLGTGSVGGGLRGSSSGG
                     SFGGGSRGSSGGGGLLAVLLALLIGGATNNSSGGTNKRGCLGRLLILVLIIAGVFLLL
                     NYCSGGNAGSFLSDLTSGYTDGSSDTGSTLSDLTGSDSGSSSGSLLDTLLGSTGVTSG
                     QISAVDGTDKVYSPHEPDTTVAAGAREKFTDVESAQTVTLMVYMCGTDLESKSGMATA
                     DLEEMASAVLNDNIRIIVETGGTSYWQNSVIKSGTNQRLRVKQGGLQMLDKNVGKKSM
                     VDPDTLSDFIQYCKNEFPADRYMLVLWDHGGGSLTGYGYDELFPNGSMSLDEIDTALK
                     NGGCKFDMIGFDACLMATMETALVTSQYGDYLIASEATEPGTGWYYTNWLSELANDPS
                     IATLDVGKTILDDYIQMSSSSSQNTLSMVDLAELSKTAPAAFSAFSSSTKELIANDGY
                     SDVASARSGARDFSAGSTKLNQIDLIHFAENLNTTEANALAEVLRASVKYNRNSTNIS
                     HANG"
BASE COUNT          590 a          775 c          771 g          511 t
ORIGIN      
        1 tccagacgta tcgccgccgc ggcgctctgc attctcttta ctttgccgct ttttctagcc
       61 tgttctcccg cggctgaacc cgccgtcgca accccggaac cgacgcaggc gaccgcaacg
      121 ccggcgccga ccgcaacgcc cgttccttcg ccgacgccgt ttcccgaacc aacgccgttt
      181 accgttgcct ggatgacgga tacgcaggaa tacgcggcca atcacaacga tattttctgc
      241 gccatgacgc agtggattgc cgatacgcaa aaggaataca acacggttct caccgtgcac
      301 acgggggata tgattcgcgg ttcctaccgc gaatatcaat gggataacat ggttcatgcg
      361 ttctcgctgc tgccgaaaga tatgcaaatt ctcaccgtcg gcggcaacca tgacatcgcg
      421 agctttatgc cggactacac gccgtatctg gcgtaccgtc cggataccga taccgatgct
      481 gagcactcga ttttcgacgg caacgtgtat tacatcgaat tcgaggcggg agatgtgagc
      541 atcctcgtgt tctccattac ctacggctac gaagtagagt cccaggattg gattaacgca
      601 gtctgcgccg agcacagcaa ctcctacgcg attctctgcc tgcatagcta tctggaaccg
      661 gcgggttact cctccggcgg caggtatctc tttgacggcg tggttaaaaa gtcccccaac
      721 gtgcggctgg tgctctgcgg gcatgagccc ggggtgcgct accagccgga agagctggac
      781 gacgacgggg acggtgttgc cgacagaacc gttcagcaga tgctcttcaa catgcaggag
      841 gagaccaagg acggagaagg ctttttacgt cttttgcagt tcgatacggt gaacgacacg
      901 ataaatgtca tcacgtattc gccgcatctg gacgtttacg gctacgactg gccgggcggg
      961 gatcacttcg gcggggaaca cctcattgaa aatgccggaa ttgcgcagta ccgcatccat
     1021 tccgacacga ctcccgtgaa ctgaaatgaa gacgttaggc ctgacaaccg cgaatatatt
     1081 tcaaaagaat tttaatcgga gggatagagt atgagcccaa ccaaccggcc gggtggcaga
     1141 acaaaaagaa ccgtgagcgg gggcggcaac gcttaccgca gaggcagcgg gctgggaacc
     1201 ggcagcgtcg gcggcgggct gagagggagc tcgtccggcg ggtcgttcgg cggcggaagc
     1261 cgcggttctt ccggcggcgg aggccttctt gccgtcctgc ttgcgctgct tatcgggggc
     1321 gctaccaaca acagctccgg cggcaccaat aagcgcgggt gcctcggacg tctcttgatc
     1381 ctcgtgctga ttatagcggg cgtattcctg ctgctgaact actgctccgg cggaaacgct
     1441 ggcagctttt tgagcgacct caccagcggg tatacagacg gttcttccga caccggcagc
     1501 acactcagcg atctgacggg cagcgattca ggttcttcct ccggttcgct gctggatacc
     1561 ctgttgggtt ccacgggcgt aacgagcggt cagatcagcg ccgtggacgg cacggataag
     1621 gtctactcgc cgcacgagcc ggacacaacg gttgccgcgg gagcgcggga aaagtttacg
     1681 gacgtggaaa gcgcccagac cgtaacgctc atggtgtata tgtgcggaac ggacctcgag
     1741 agcaagagcg gtatggcgac cgccgacctc gaagagatgg caagcgccgt gttgaacgac
     1801 aacatccgca tcatcgtaga gacgggcggt acttcctact ggcagaacag cgtaatcaag
     1861 agcggcacga accagcgtct ccgcgtaaag cagggcggcc tgcagatgct cgataaaaac
     1921 gtaggcaaaa agtccatggt cgacccggac acgctctcgg acttcataca gtactgcaaa
     1981 aacgagttcc cggcggatcg ctacatgctc gtgctctggg atcacggcgg cggctcgctg
     2041 actgggtatg gctacgacga gctcttcccg aacggctcca tgagtctgga cgagatcgac
     2101 accgcgctga aaaacggcgg ctgcaagttc gacatgatcg gttttgacgc gtgcctgatg
     2161 gcgacaatgg aaactgcgct ggtaaccagc cagtacggcg attacctgat cgcttccgaa
     2221 gcgaccgagc cgggcaccgg ctggtactat accaactggc tcagcgagct ggcaaacgat
     2281 ccgtctattg ctacgctgga tgtaggcaag acgattctgg acgactacat ccaaatgagc
     2341 tcgtcctcca gccagaacac gctttccatg gtcgatctcg cggaactctc caaaaccgct
     2401 ccggcggcgt tttctgcgtt ttcctcctcg accaaggagc tgattgccaa cgacggatat
     2461 tccgacgtgg cgagcgccag aagcggcgcg cgcgatttct cggcgggttc gacaaaactc
     2521 aaccagatcg atttgatcca ctttgccgag aacctcaaca cgacggaagc caacgcgctg
     2581 gcggaagttc tgcgcgcgag cgtaaaatac aaccgcaact cgacgaatat ttcgcacgcc
     2641 aacggac
//
LOCUS       DAOMGY010000176         2646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_42335_length_2646_cov_3.221536, whole genome shotgun sequence.
ACCESSION   DAOMGY010000176 DAOMGY010000000
VERSION     DAOMGY010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42335_length_2646_cov_3.221536"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1063
                     /locus_tag="PLR57_03560"
     CDS             179..1063
                     /locus_tag="PLR57_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40564.1"
                     /translation="MPQIIREMEQGKVSVSLPSLRVDSELANDLSVMQSVRKAGLTFA
                     PEAGTQRLRDVINKNVTEEDILRATQDAFSAGWSSVKLYFMLGLPTETDEDILGIAAL
                     SRKVSQQFYSLPKEQRGKGLRLTVSVSTFVPKPFTAFQWCGQVPAEEIRRRQELLKNA
                     IRGIRGVELHCHFSELSVLEAAFSRGDEKLAGVLKRAYELGCRFDAWTEHFQPKAWAQ
                     AFSDCELSAADYAERAYDLEEPLPWDYVDMLVTKDYLKKEFLRSQQEETTPDCREHCH
                     ACFGQRTDADCLSLKESK"
     gene            1128..1811
                     /locus_tag="PLR57_03565"
     CDS             1128..1811
                     /locus_tag="PLR57_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HQQ40565.1"
                     /translation="MKLVAVYHKGRALAMISHLDVQRTLQRAFRRAGLPLLYSNGFNP
                     HPQFSFATAAATGMSSDCEWFEVQLFEPIAPEEFVRRANAAMPDGMSVSDAFEPPEGF
                     GSLSAKLRAALYRAEISFETPVDGEKLKQTLETMLSGEIVVNKRTKSGIRPVDMRPYI
                     LEVSVEEVGDGIAVLRVLGKLQADGGLRVDAFIDALLERLDAQATYALRRMRMYFAGD
                     GFLPRLPSE"
     gene            1828..>2646
                     /locus_tag="PLR57_03570"
     CDS             1828..>2646
                     /locus_tag="PLR57_03570"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HQQ40566.1"
                     /translation="MNKEILVDATGFATRVAVVEDGAPVELYVEQEGTERLVGNIYNG
                     KVQNVLPGMQAAFIDIGLERNAFLYAGDIVLSDDPDDEIEQVVDSRNIRDLLKPGQTI
                     MVQVAKDPVGTKGARVTTHITLAGRTLVLMPTMDYVGVSRRIDKDDERQRLRKILTEI
                     KPKGMGVIVRTASAGKPKEAFVEDMESMVKLYREIEKKAASKKAPALLHAEQSLLFRT
                     VRDLLMRDIDRVFVNDTCCYDQLREIADVMAPKLKARILLKESEALFDRYETETK"
BASE COUNT          582 a          744 c          813 g          507 t
ORIGIN      
        1 gcgcggctgc acgcgcggct gccgcttctg ccaggcgggg tatatctacc gtccggtgcg
       61 cgaacgcaag agcgaaacgc tcatgcggca ggcgcgctag cagatcggct gcacgggtta
      121 cgactaagta tcgcttttaa gcctcagtac gggggattat tccgaggtgc atacgctcgt
      181 gccgcagatc atccgcgaaa tggaacaggg caaagtttcc gtgtccctgc cttcgctccg
      241 ggtggattct gaactggcaa acgatctttc cgtcatgcag agcgtgcgga aagcaggctt
      301 gacctttgcg ccggaagcgg gcacgcagcg tttgcgggac gtgatcaaca agaacgtcac
      361 ggaggaagat attctccgtg ctacgcagga cgcgttttcc gcgggctgga gcagcgtcaa
      421 gctctatttt atgctcggcc tgccaaccga aacggacgag gacattctcg gcatcgcggc
      481 gctttcgcgc aaggtctccc agcagttcta cagcctgcca aaggaacagc gcggcaaggg
      541 gctgcggctc accgtgagcg tttctacgtt cgtgccaaag ccgttcacgg cgtttcagtg
      601 gtgcgggcag gtgcctgccg aagagattcg ccgccgtcag gaactgctca aaaacgcgat
      661 tcgcggcatt cgcggcgtgg aactgcactg tcacttttcc gagctttccg tgctggaagc
      721 ggcgttttcg cgcggggacg aaaagctcgc gggcgtgctc aagcgcgctt atgagctggg
      781 ctgccggttc gacgcttgga cggagcattt tcagcccaag gcgtgggcac aggcgttttc
      841 cgattgcgaa ctcagcgctg cggactatgc cgaacgcgcg tacgatctgg aagagccatt
      901 gccgtgggac tatgtggata tgctggtaac aaaggactat ctcaaaaagg aattcctccg
      961 ttctcagcag gaagaaacca cgccggactg ccgcgagcac tgccatgcgt gctttggaca
     1021 acgaacggac gcggactgcc tctcgctgaa ggaatcgaaa taacgccgtc gtcccgcgaa
     1081 aagataacgc gagatgacgg atgactgaat aagaatcgga gcagaccatg aaactagttg
     1141 ctgtttacca taaaggacgc gcgcttgcga tgatttcgca tctcgacgtg cagaggacgc
     1201 tccagcgcgc gtttcgcagg gcgggattgc cgctgttgta ttccaacggc tttaacccgc
     1261 atccccagtt ttcgtttgca accgccgccg ccaccggcat gtccagcgac tgcgagtggt
     1321 tcgaagttca gttgtttgaa cccatcgcgc cggaggaatt tgtccgccgg gcaaatgcgg
     1381 ccatgccgga tggcatgtcc gtttcggacg cgttcgagcc gccggaaggc ttcggctcgc
     1441 tttcggcaaa actccgcgcg gcgctctacc gcgccgagat cagctttgaa actcccgtag
     1501 acggggagaa attgaaacaa acgctcgaaa ccatgctttc gggcgaaatc gtagtaaaca
     1561 aacggacaaa gagcgggatt cgtcccgtag acatgcgtcc gtatattctg gaggtctccg
     1621 tggaagaagt cggggacgga attgccgtcc tccgggttct cggcaaactg caagccgatg
     1681 gcgggctgcg ggtcgatgcg ttcatcgacg cgttgctcga acgtttggat gcacaggcga
     1741 catacgctct ccgccgtatg cgcatgtact ttgccggtga tggctttttg ccgcgcttgc
     1801 cttccgaata agaaagagaa ggtaaaaatg aataaggaaa tcttggttga cgcaaccggc
     1861 ttcgccacgc gcgtggcggt tgtcgaggac ggggcgcccg tcgaactcta cgtcgagcag
     1921 gagggcaccg agcggctcgt cggcaacatc tacaacggca aggtgcaaaa cgtgctgccc
     1981 ggcatgcagg cagcgtttat cgacatcggg ctggaacgaa acgcatttct ctacgcgggc
     2041 gacatcgtgc tttcggacga tccggacgac gaaattgaac aggtggtgga ctcgcgcaac
     2101 atccgcgatc tgctcaagcc cgggcagacc atcatggtgc aggtggcaaa agaccccgta
     2161 ggcaccaagg gcgcgcgcgt aaccacgcat atcacgctgg ccggccgcac gctggtactc
     2221 atgccgacaa tggattacgt aggcgtttcc cgccgaatcg acaaggacga cgagcgtcag
     2281 cgcctccgca aaatcctcac cgaaatcaaa ccgaagggca tgggcgtgat cgtccgcacc
     2341 gcgtccgcgg gtaaacccaa ggaagcgttt gtcgaggaca tggagagcat ggtcaagctc
     2401 taccgcgaaa ttgaaaagaa ggccgcgagc aaaaaagcgc ccgcgctttt gcatgccgag
     2461 cagtcgctgc tgtttcgcac cgtgcgcgac ctgctgatgc gggatatcga ccgcgtgttc
     2521 gtaaacgaca cgtgctgcta cgaccagctg cgggaaattg cggacgtgat ggcgccaaag
     2581 ctcaaggcgc gtatcctgct caaggagagc gaagcgctct ttgaccgtta cgaaacggaa
     2641 acaaag
//
LOCUS       DAOMGY010000177         2645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_42377_length_2645_cov_2.803475, whole genome shotgun sequence.
ACCESSION   DAOMGY010000177 DAOMGY010000000
VERSION     DAOMGY010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42377_length_2645_cov_2.803475"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="PLR57_03575"
     CDS             <1..905
                     /locus_tag="PLR57_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HQQ40567.1"
                     /translation="EALAALGETAVYDRTPADEILARIGDAEATYTNKTPLTRETIQA
                     CPNLKFIGVLATGYNVVDVAAAKERGIPVCNVPTYGTAAVGQFAIAMLLEICHHVAHH
                     SEAVHEGRWSGNIDWCFWDYPLIELEGKTMGVIGFGRIGQRTGEIAKALGMRVLAYDA
                     YQNDAGKAIGTYVDLDTLLKESDVISLHCPLFPETKGIVNAASIAKMKNGVILLNNSR
                     GPLIVEQDLADALNSGKVYAAGLDVVSEEPISPNNPLLSAKNCLITPHISWAPKESRA
                     RLMNIAVDNLSNWQNGKPTNVVNA"
     gene            913..1842
                     /locus_tag="PLR57_03580"
     CDS             913..1842
                     /locus_tag="PLR57_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQQ40568.1"
                     /translation="MKLKGVLYAVLSAVIFGTSPIFFKTIAATGINTTTQVVVRSVIT
                     MAIAAALAAAKKQPVLLKGRMLVDALLAFVAGQGVTAVLLNSSYAYLSAGMSTSLHFV
                     YPSVVMLVSVLFFRERVNLLKFAALLLSVFAIALMTDFSARGQLTGMLLALASGCSYA
                     FYILYLDRTALKDIPVWTFAFWSSLGCLVASAGLGIFTNTLDLSDATVKGVSLLLIMV
                     PLQSVLAVRLFQLGVRYTGSTAASLLSTMEPVTSTILGILLLHEKTDIGKLFGCAAIV
                     LSVVLVVLGTKRAEKRALAVGLEPSTEPARETE"
     gene            1880..>2645
                     /locus_tag="PLR57_03585"
     CDS             1880..>2645
                     /locus_tag="PLR57_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ40569.1"
                     /translation="MIDLTKWERIASEQIHNRKAAAGRLSGKIVIVTGSAQGFGKGIA
                     EELYREGASVLVADLNEPLAKAVAESLGERASSVRVDVTDEASVAAMIAACVETFGGL
                     DLFISNAGVLKAGSLDELEKADFEFVTSVNYTAFFTCVKYAQRIMRAEFAADESWMGD
                     IIEINSKSGLEGSGKNFAYAGGKFGGIGLVQSFALELAPYNIKVNAVCPGNYYDGPLW
                     SDPERGLFVQYLRAGKVPGAKTVEDVKNYYLSKPLIK"
BASE COUNT          525 a          738 c          832 g          550 t
ORIGIN      
        1 gggaagcgct tgcagcgctc ggtgaaacgg cggtgtacga ccgcacaccc gcagatgaaa
       61 ttctcgcgcg catcggagac gcggaagcga catataccaa caaaacgccg ctgacgcgcg
      121 aaacgattca ggcctgcccg aatctgaagt ttatcggcgt actcgctacg ggctataacg
      181 ttgtggacgt cgccgcggca aaagagcgcg gcatcccggt ctgcaacgtt cccacctacg
      241 gcaccgctgc ggtggggcag tttgccattg ccatgctgct ggaaatctgc caccacgttg
      301 cgcaccacag cgaggctgtg cacgaaggcc gctggagcgg caacatcgac tggtgcttct
      361 gggactatcc gcttatcgag ctggaaggca agaccatggg cgtgatcggc tttggccgca
      421 tcggccagcg cacgggcgag atcgccaaag cgctcggcat gcgggtgctt gcttacgacg
      481 cgtatcagaa cgacgcggga aaagcgattg gaacgtatgt cgatctggac acgctgctga
      541 aagagtccga cgtaatcagc ctgcactgcc cgctctttcc tgagacaaag ggaatcgtca
      601 acgccgcttc gattgcgaag atgaagaacg gcgttatcct cctcaacaac agccgcgggc
      661 cgctgattgt ggagcaggat cttgccgacg cgctgaacag cggaaaggtc tatgccgcgg
      721 gcctggacgt tgtttccgaa gagccgattt cgccgaataa tccgctgctt tcggccaaga
      781 actgcttgat cacgccgcat atcagctggg cgccgaaaga aagccgcgcg cgtttgatga
      841 acattgccgt ggataacctg tccaattggc agaacggcaa gccgacgaac gtcgtcaacg
      901 cctgaggagt ttatgaagtt aaagggcgta ctatacgctg tactttcggc ggtgatcttc
      961 ggcacgtcgc cgatcttttt taagaccatt gccgctaccg gcatcaacac cactacgcag
     1021 gtagtggtgc gctccgtcat caccatggcg attgcggcgg ctctcgcggc ggcaaaaaag
     1081 cagccggtcc tgctcaaagg caggatgctt gtggacgctc tgctcgcgtt tgttgcgggg
     1141 cagggcgtaa cggctgttct gctcaactcg tcgtacgctt acctttccgc gggaatgtcc
     1201 acctcgctgc attttgtgta tccttcggtc gtgatgctcg tgagcgtgct gttcttccgc
     1261 gagcgggtca accttctcaa gtttgcggcg ctgctgctga gcgtttttgc catcgcgctg
     1321 atgaccgact tcagcgcgcg cggccagctt accggaatgt tgctggcgct cgcttcgggg
     1381 tgcagctacg cgttttacat tctgtatctc gaccgcacgg cgctcaagga tatccccgtc
     1441 tggacgttcg cgttctggtc gtcgctgggg tgtctggttg cttcggcggg gcttggcatt
     1501 ttcaccaaca cgctggatct ttccgacgcg acggtcaagg gcgtttcgct gctgctgatc
     1561 atggtgccgc tgcagagcgt gctggcggtg cggctgtttc agctcggcgt gcggtacaca
     1621 ggctcgacgg cggcttcgct gttgagcacc atggaacccg ttacgagcac gattctcggc
     1681 attttgctgc tgcacgaaaa aacggatatc ggcaagctct ttggctgcgc cgccatcgtg
     1741 ctctccgtcg tgctcgtcgt gctcggcaca aagcgcgcgg agaagcgggc tttggcagtg
     1801 ggtttggaac caagcacgga gccggctcgg gagacggaat aatcccaaat tcccgttcgg
     1861 aacacggagg aggaattgga tgattgacct aacaaaatgg gagcggatcg cttccgaaca
     1921 gattcataac cgaaaagcgg ctgcgggtcg cttatctggc aagatcgtaa tcgtgaccgg
     1981 cagcgcgcag ggattcggca agggcattgc cgaggaactg taccgcgaag gcgcgagcgt
     2041 gctcgttgcc gacctcaacg aaccgctggc aaaggccgtt gcggagtcgc tgggcgagcg
     2101 agcctcttcg gttcgcgtgg acgtgaccga cgaggcgagc gttgcggcaa tgatcgccgc
     2161 gtgcgtggag accttcggcg gtctggactt attcatcagc aacgcgggcg tgctcaaggc
     2221 gggctcgctg gacgagctgg agaaagcgga ctttgagttt gtaacgagcg tcaactacac
     2281 cgcgttcttc acctgcgtca aatacgccca gcgtatcatg cgcgccgagt ttgcggcgga
     2341 tgagagctgg atgggtgaca tcatagagat caacagcaaa tccggcctcg aaggcagtgg
     2401 caagaacttc gcctacgcgg gcggcaagtt cggcggcatc gggcttgtgc agagctttgc
     2461 gctggagctt gctccgtata atatcaaggt caacgccgtc tgtcccggca actactacga
     2521 cgggcctctc tggagcgatc cggagcgcgg cttgtttgtg cagtacctgc gcgcgggcaa
     2581 ggtgccggga gcaaagacgg ttgaggatgt aaagaactac tatttgagca agcctctgat
     2641 taagc
//
LOCUS       DAOMGY010000178         2644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_42393_length_2644_cov_4.123986, whole genome shotgun sequence.
ACCESSION   DAOMGY010000178 DAOMGY010000000
VERSION     DAOMGY010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42393_length_2644_cov_4.123986"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..1133)
                     /locus_tag="PLR57_03590"
     CDS             complement(231..1133)
                     /locus_tag="PLR57_03590"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HQQ40570.1"
                     /translation="MISFSPITIQNAMELKPYLDVQPFRTCDYTLGAVFQWRAYFHSA
                     YAIVHGMLVMLASYPNECINCFVYPVGSGDLYAALDEIEAHAKETGMPLTFCAVPEEG
                     LAVLSERYGERAIVSTHRNWADYLYNASDLVDFPGKQYHTQRNHLNRFFKDNPSAQYV
                     PVTEETLPAALKFLNEYEQCAPVDKVIEAEEMLRAKELLYDALKLGQKAGYIDVGGTI
                     VALAIGEVVSDTLYVHVEKARVDYAGAYQAIVSLFAKNALEEGTLYINREDDSGEERL
                     RYSKMSYRPIRLIDKYWVTLENPA"
     gene            1534..1974
                     /locus_tag="PLR57_03595"
     CDS             1534..1974
                     /locus_tag="PLR57_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40571.1"
                     /translation="MNRITVPRLRAEAKKQLLSYRRNNARAYKNEAQRAERVNQTGWT
                     RAIDEARAYLGAVNPTKERFMCRLFGLETPVPRCRTARERMIKLSMELTVSESTLYKW
                     REDILEIVLYAAIEAGLVCPFGIRAGETNAEPKPERTKCAKAKN"
     gene            2043..>2644
                     /locus_tag="PLR57_03600"
     CDS             2043..>2644
                     /locus_tag="PLR57_03600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HQQ40572.1"
                     /translation="MEKIIQTELGPLTVNITRKRMKNLYLRVLPPDGTLQISAPNRVG
                     DLEIEQFVLSRLDWIARQREKVTSRKSVHFADGETLPFFGTNLTLRLRPVTGRAKVEQ
                     TGGMLILSIRADADTTARKRAVDGWYRRELTSAAEAMLPACEQTVGKRASELRIRDMK
                     TRWGTCNIKSGAVTINLRLAEKPPECLRYVLTHELCHLHEA"
BASE COUNT          630 a          710 c          773 g          531 t
ORIGIN      
        1 tattgaatgt gatgggcgac tgcatcgcgt agccacccag aatgcaggca acgaacgtaa
       61 tcagcacgac ccaccagtag cggccgcgca gattgtccca agcaatctga cgataatcgc
      121 tggctcttct catttaatat ttccccccca aaagtttcat atcgttgacc gcggaactgc
      181 cgcgggaaac gccttggctg atgcgcatat ttcgcgcatt cgtttgcagg ttacgcgggg
      241 ttttccagcg taacccagta tttatcgatc agccggatcg gccggtagga catcttggaa
      301 taccggagcc gctcctcgcc ggaatcgtcc tcgcggttga tgtagagcgt gccttcctcc
      361 agcgcgttct ttgcaaacag cgacacaatc gcctgatacg cgccggcgta atcgacgcgg
      421 gctttctcca cgtgcacata cagcgtgtcc gaaacgactt cgccaatggc gagggcgacg
      481 atggtgccgc caacgtcgat atatccggct ttttggccga gcttcagcgc gtcgtaaagc
      541 agttccttgg cgcggagcat ctcttccgcc tcgatgacct tgtccacggg cgcgcactgc
      601 tcgtattcgt tcaggaattt aagcgccgcg ggaagcgttt cctccgtaac cggcacgtac
      661 tgcgcggaag gattgtcctt gaagaagcgg ttcagatggt tgcgctgggt atggtactgc
      721 ttgccgggaa aatcgacgag gtcggaagcg ttgtagagat aatccgccca attgcggtgc
      781 gtggagacga tggcgcgctc gccataccgt tcggagagaa cggcaagccc ctcttccggc
      841 acggcacaga aggtcagcgg catgccggtt tcctttgcgt gcgcttcaat ttcatccagc
      901 gcggcgtaca gatccccact tccaaccggg tagacaaagc agttgatgca ttcgttgggg
      961 tagcttgcca gcatgacgag catgccgtga acgatggcgt atgcggaatg gaaataagct
     1021 ctccattgaa acaccgcgcc gagcgtatag tcgcaggtgc ggaaaggctg aacgtccaga
     1081 tacggcttga gctccattgc gttctggatc gtgatcggtg aaaaagatat cataactccc
     1141 ctaatgattg tcagtcatca aaacaaacta aattcagcgt cgcgatgcat tccacctgct
     1201 gttcgcggcg cggttgtttt tactgcacta tgataccact aaacctctgc gtcaggcaat
     1261 tgcgggtatt gggaattccg cccggttttg agggacgatt tgtaaaatta tggaagcgaa
     1321 atcgtgaact cgcgccgaac gccaaaacca ctttgtaaac aggtgaggcg aatggcgttg
     1381 caaaaccgaa agaaaacgac aaaatgcgat attatgcgag aaaataattc gaaatatttc
     1441 tgctttacgc aagaaaaacg agtacaaaac gcgcgtcatg cgttataata tacgtacaaa
     1501 cgttcgcatt gatgcgaaca gcgggaggaa attatgaacc ggattacggt gccccgcctg
     1561 cgcgcggaag caaagaaaca gctgctgagc tacagaagga acaatgcccg cgcttataaa
     1621 aacgaagcgc agcgcgccga gcgcgtgaat caaaccggct ggacgcgcgc aatcgacgaa
     1681 gcgcgcgcct atcttggcgc ggtcaatcca accaaagaac gctttatgtg ccgtttgttc
     1741 gggctggaaa cgcccgtgcc gcggtgccgt accgcgcgcg agcggatgat taagctgagc
     1801 atggagctta cggtttccga atcgacgctc tacaaatggc gcgaggatat tctggaaatc
     1861 gtgctttacg ccgccattga agcggggctg gtctgcccgt tcggcatccg cgcgggggag
     1921 acgaacgcgg agccgaagcc tgagcggacg aagtgcgcaa aggcgaagaa ttgacggcgc
     1981 tggacgcgga atggtagaat gctctcgaac tgcggaaaac attctgagcg gaggcgggga
     2041 agttggagaa gatcattcaa acagagcttg gcccgctgac ggtcaatatc accagaaagc
     2101 gcatgaaaaa cctctatctg cgcgtgctgc cgccggacgg aacgctgcag atcagcgcac
     2161 cgaaccgggt cggcgacctg gagatcgagc agtttgtgct gagccgcctc gactggattg
     2221 cccgccagcg cgagaaggta acgagcagaa agagcgttca ttttgcggac ggtgaaacgc
     2281 tgcccttctt cggaacaaat ttgactctgc gcctgcgtcc cgtaaccggg cgcgctaagg
     2341 tggagcagac aggcggaatg ctcattctat ctatccgcgc ggatgcggat acaacggcgc
     2401 gaaagcgcgc cgtcgacggc tggtaccgcc gcgaactgac ttccgccgcc gaggcaatgc
     2461 tgcctgcctg tgagcaaacg gttggcaagc gtgcttcgga actgagaata cgggatatga
     2521 agacgcgctg gggcacctgc aacatcaagt ccggcgcggt gaccatcaac ctgcggctgg
     2581 cggaaaaacc gccggagtgc ttacggtacg tgctgacgca tgaactgtgc catctgcacg
     2641 aagc
//
LOCUS       DAOMGY010000179         2631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_42682_length_2631_cov_4.181289, whole genome shotgun sequence.
ACCESSION   DAOMGY010000179 DAOMGY010000000
VERSION     DAOMGY010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42682_length_2631_cov_4.181289"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="PLR57_03605"
     CDS             complement(<1..1204)
                     /locus_tag="PLR57_03605"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQQ40573.1"
                     /translation="MKQVLCLAGAAIFLVCAPAFSRAELPIADDVTELCEVTEPSPGR
                     AARRLTDGNVETYTEFSEFKDFRCVWGEDAPVAGMYLKFYQAPGSFVLTQLDAAGEAL
                     SEQTVESPLLNAYYPLETAARGVLIHVNENMSIGEVAFYSAGSLPEGVHDWQPPADKA
                     DLLVISAHCDDELLYFGGTIPTYAGEKGLVVQVAYMANEERLRMDEAMNGLWLCKVRN
                     APVLVGMKDAYTETLEAAEKRWGRDETVAAIVSLIRRFKPEVIVTHDLEGEYGHGAHR
                     ITASCTLEAVSAAADGTLYPESAQKYGAWQTKKLYLHLFDTGAITMDWRVPLAAFDGL
                     TALDVANLAYDCHVSQQEFHQNVYDTGDFSSAEYGLAYTQVGQDVAGGDFFENIPLDA
                     LSNYVAPTP"
     gene            complement(1366..>2631)
                     /locus_tag="PLR57_03610"
     CDS             complement(1366..>2631)
                     /locus_tag="PLR57_03610"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ40574.1"
                     /translation="YDSFLPDVTTDERMDRVSGWGYAAGYIGGSTIPFVISIAVMLLR
                     NYDPIAMKFAILIVTVWWLLFSIPILKNVKQTHYVETPPNELAKHAMSNLWDTMKRCV
                     SNKGLFIYLIAYFLYIDGVGAVISLATNYGATLGLGTTGMIFALLVTQLVAAPCAILF
                     SKLSGKFGAIKMIISAIIMYFIICGVGFFMGRLVEPYQLDYVSAARTSIVAQGVAFDN
                     KEDEKAWSSLTVDLVEDTRDVLSAEDRAAAFDEVVADYIRQAGDATAVNYVFTSDASR
                     QTAVTALTNIGADLDVYAADTAKQEAYNGARTTATTLFWVLAALVGTVQGGIQALSRS
                     YYGKLVPQSRSAEYFGFYDVFGKFATVIGPLLYSMFYFLTDRASVGILSLLILFGGGG
                     ILLIAGRKELSKTEKEMRAANAVMEAEEA"
BASE COUNT          537 a          793 c          768 g          533 t
ORIGIN      
        1 aaggcgtcgg cgcaacgtag ttggacagcg catccaacgg gatgttctca aagaagtccc
       61 cgcccgctac atcctgcccc acctgcgtat aggcaagtcc gtattccgcg ctggaaaaat
      121 cgcccgtatc gtacacgttt tgatggaact cctgctggga aacatggcag tcgtaggcaa
      181 ggttggcaac gtccaacgcg gtgagcccgt cgaacgcggc aagcggaacg cgccagtcca
      241 ttgtgatggc gccggtatcg aacaaatgca gatacagctt cttggtctgc catgcgccgt
      301 atttttgtgc ggattccggg tagagcgtgc cgtccgccgc ggcggagacc gcttcgagcg
      361 tgcagctcgc ggtaatgcgg tgcgcgccgt ggccgtactc gccttcgagg tcgtgggtca
      421 cgatgacttc tggtttgaac cggcggatca gcgaaacgat ggcggctacg gtttcatccc
      481 gtccccagcg cttttcggcg gcttccagcg tttcggtata cgcgtccttc atgccaacca
      541 gtacgggcgc gtttcgaact ttgcagagcc aaagcccgtt catggcttcg tccatgcgca
      601 ggcgctcttc gtttgccata tacgcgacct gcacgacgag gcccttttca cccgcatacg
      661 tgggaatcgt cccgccaaag tagagcagtt cgtcgtcgca gtgcgcggaa atcaccagca
      721 ggtccgcctt atcggcaggc ggctgccagt cgtgaacgcc ttcaggcaaa cttccagccg
      781 agtagaaggc gacttcgccg atggacatgt tttcgtttac gtgaatcagc acgccgcgcg
      841 cggctgtttc cagcgggtaa tatgcattga gcagcgggga ctcgaccgtc tgctccgaga
      901 gcgcttctcc cgcggcgtcc agctgagtca gcacgaacga acccggcgcc tggtagaact
      961 tgagatacat gcccgcaacg ggcgcgtcct caccccagac gcagcggaag tccttgaact
     1021 ccgaaaactc tgtgtaggtt tccacgttcc cgtcggtcag gcggcgcgcc gctctgccgg
     1081 gggaaggctc ggttacttca caaagttccg tcacgtcatc cgcaatggga agctccgcgc
     1141 gcgaaaacgc cggcgcgcag acgagaaaga tcgccgcgcc ggcaagacag agaacttgtt
     1201 tcaaacggga aagcgtcatt tccgtatgtc ggtttccttc cgctataatg cgttcggcag
     1261 ctgcgccgaa cagaaactcc aacttaccct attatacacg attcccgcgc cgaggaaagg
     1321 ggattacccc gtaacgcggc gcgggaattg tttaaacgct tctttttatg cctcttcggc
     1381 ttccataacc gcattggccg cgcgcatctc cttctcggtt ttgctgagct ccttgcgccc
     1441 ggcaatcagc agaatgccgc ccccgccaaa gagaatcagc aagctcagaa tacctacgct
     1501 cgcgcggtcg gtgaggaaat agaacatgga atacagcagc ggccctataa cggttgcgaa
     1561 cttgccgaac acgtcgtaga agccgaaata ctccgcgctt ctgctttgcg gaaccagctt
     1621 gccgtagtag gagcgggaca gcgcttgaat gccgccctgc acggtgccga ccagcgcggc
     1681 gagcacccag aagagcgttg tcgccgttgt gcgcgcgccg ttatacgctt cctgcttggc
     1741 cgtatccgca gcgtatacgt ccagatccgc tccaatattc gtcagcgcgg tcacggcggt
     1801 ctggcggctt gcgtcgctcg taaagacata gttcacggcg gtggcgtccc ccgcctgacg
     1861 gatatagtcc gcaacaactt cgtcaaaggc cgccgcgcgg tcttccgcgg agagcacgtc
     1921 ccgcgtgtcc tccaccagat cgacggtcag gctgctccag gctttttcgt cttccttatt
     1981 gtcaaacgcc acgccctgcg cgacaatgct cgtgcgcgcg gcggagacat agtcgagttg
     2041 gtacggttcg accagccgcc ccataaagaa gccgacgccg cagatgataa agtacatgat
     2101 aatcgccgag atgatcatct tgatcgcgcc gaatttgccg gagagtttgc tgaacagaat
     2161 ggcacagggc gccgcgacca gctgggttac cagcagtgca aagatcatgc cggtcgtgcc
     2221 gaggccgagg gttgcgccgt agttcgttgc cagcgaaatc accgcaccga caccgtcgat
     2281 atagaggaag tacgcgatga ggtagatgaa taagcccttg ttgctcacgc agcgcttcat
     2341 cgtgtcccag aggttcgaca tggcgtgctt tgccagctcg ttcggcgggg tttccacata
     2401 gtgcgtctgc ttgacgtttt tgaggatcgg aatcgagaac agcagccacc agaccgtgac
     2461 gatgaggatg gcgaacttca tggcgatggg atcgtagttc ctcagcagca tgaccgcgat
     2521 ggagatcaca aacggaatcg tgctgccgcc gatatagccc gcggcgtatc cccagccgga
     2581 gacgcggtcc atgcgttcgt cggtcgtaac gtccggcaaa aacgagtcgt a
//
LOCUS       DAOMGY010000180         2631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_42692_length_2631_cov_2.952640, whole genome shotgun sequence.
ACCESSION   DAOMGY010000180 DAOMGY010000000
VERSION     DAOMGY010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42692_length_2631_cov_2.952640"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /gene="xseA"
                     /locus_tag="PLR57_03615"
     CDS             <1..583
                     /gene="xseA"
                     /locus_tag="PLR57_03615"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQQ40575.1"
                     /translation="DLWAFNEPEVAEAIVQSRIPVISAVGHETDFTIADFAADLRAPT
                     PSAAAELAVPEMSACYDAVSSLSERMRRALTASLERMRSNVKLFASSSAFRLLERRVQ
                     SERQTLDIMGERALRAAKERLKSERTALRTQSAKLTALGPGAVLERGYAILTDSAGNT
                     VTGVSKLKPKDIVGVRMRDGTAEATIERVEPNA"
     gene            624..836
                     /gene="xseB"
                     /locus_tag="PLR57_03620"
     CDS             624..836
                     /gene="xseB"
                     /locus_tag="PLR57_03620"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HQQ40576.1"
                     /translation="MEQPLRFEDAMQKLSGIVEKLESGEGSLDEMLRLYEEGMKLVKA
                     CETQLDAYEATITKLGQGAGGGEDAE"
     gene            826..1668
                     /locus_tag="PLR57_03625"
     CDS             826..1668
                     /locus_tag="PLR57_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQQ40577.1"
                     /translation="MLNDRLARYAAMTEQKLGELLGDQEIPALLKESMAYSVFSGGKR
                     LRPSLCMAACELCGGASEDALLPACAVELIHTYSLIHDDLPAMDNDDMRRGKPSSHIA
                     FGEANAILAGDGLQALAFAILADCPAVGAQKHIARGAFEMVMGQSLDCNTEGKAELLN
                     TLQDYKTGALFRAAVGAGAACAHASKEQTNALLAFATAYGMLFQITDDILDFTGSAKA
                     LGKSVGKDAAAGKVTYVTVYGLEGAKERANMFAEEARQAVLNAGGDAAFFLELIDYTL
                     TRRA"
     gene            1712..>2631
                     /locus_tag="PLR57_03630"
     CDS             1712..>2631
                     /locus_tag="PLR57_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HQQ40578.1"
                     /translation="MDRYPILDNINSPEDVKKLPEGQLPVLAAQIRQYMIENVARCGG
                     HLASSLGAVELTIAMHRAFSSPTDEIVFDVGHQSYAHKILTGRREAFCTLRQENGLSG
                     FPKREESEHDPFNTGHASTAISAALGIARAKRLRGQEGAAIALVGDGALTGGLAFEGL
                     NDAGQADVPLIVILNDNEMSISQNVGALHRMLVNMRVSPGYVRFKRFLVRVLDTGILG
                     RWLSRHMENLKNRIKSFLMPKRLFEDMGFLYLGPIDGHDIPALTRMFRRAKELKVPVI
                     VHCLTQKGKGYAFSERNPEKYHGVAPFSIDT"
BASE COUNT          589 a          744 c          820 g          478 t
ORIGIN      
        1 agacctttgg gcgttcaacg aacctgaggt tgccgaagct attgtgcaga gccgaatacc
       61 ggtcatcagt gcggtcgggc atgaaacgga ctttaccatt gcggatttcg ctgcggatct
      121 tcgcgcgcct acgccttccg ccgcggcgga gcttgccgtg ccggagatga gcgcctgcta
      181 cgatgcggta agctcgctct cggaacgcat gcgccgcgcg ctcacggctt cgctggaacg
      241 tatgcgctcg aatgtaaaac tgtttgcctc ctccagcgcg tttcggctgc tggaacgcag
      301 ggtgcagtcc gagcggcaaa cgctcgacat aatgggcgaa cgcgcgctgc gcgcggcgaa
      361 agaacgttta aagagcgaac gaaccgccct gagaacacag tcagccaagc tgaccgcgct
      421 cggccccggc gcggtgctgg aacggggata cgctatttta actgacagcg cgggcaacac
      481 cgtgaccgga gtgagtaaac tcaaaccaaa ggatattgtc ggcgttcgca tgcgtgacgg
      541 cacggcggaa gcgacgattg aacgggtaga accaaacgcc tgaacacgat tccgcaaatg
      601 cggaggcggg gaggataaat tcgatggaac agccattgcg ctttgaagac gcgatgcaga
      661 aactttcggg catcgttgaa aaactggaaa gcggagaagg gtctcttgac gagatgcttc
      721 gcctttacga agaaggtatg aagctcgtca aggcctgcga aacgcagctc gacgcttacg
      781 aagcgaccat caccaagctc ggtcagggcg ctggaggcgg cgaagatgct gaatgaccgt
      841 cttgcacgct acgccgcgat gacggaacaa aagctgggcg agttgcttgg cgatcaagag
      901 atcccagcgc tgctgaaaga gagcatggcc tacagcgtgt tttccggcgg aaagcgtctg
      961 cggccttcgc tctgcatggc ggcctgcgag ctctgcggtg gagcgtcgga ggatgcgctc
     1021 ctgcccgcgt gcgcggtgga actcatccac acctattcgc tcattcacga cgatctgccc
     1081 gccatggaca acgacgatat gcggcgcggc aaacccagca gccacatcgc ctttggggaa
     1141 gccaacgcaa ttctggcggg ggacgggctg caggcgctgg cgttcgcgat tctggcggat
     1201 tgccccgcag tcggtgcgca gaagcatatt gcgcgcggcg cgtttgaaat ggtcatgggg
     1261 cagagcctcg actgtaatac cgaaggcaag gcggaactgc ttaacacctt gcaggactat
     1321 aaaaccggcg cgctgtttcg cgcggcggtg ggcgcgggcg cggcttgcgc gcatgccagc
     1381 aaggagcaga caaacgcgct gcttgcgttt gcaaccgcct acggcatgct gtttcagatt
     1441 acggacgaca ttctggactt caccggcagc gccaaagcgc tcggcaagtc cgtcggcaaa
     1501 gacgcggcgg cgggcaaggt gacatatgtt accgtttacg ggctggaagg cgcaaaagaa
     1561 cgcgccaaca tgtttgccga agaggcaaga caagccgttt tgaatgcggg cggagacgct
     1621 gcgttctttc tggaactgat cgactacacg cttacgcggc gtgcgtaaga aaacggaata
     1681 aaccggacga cgaaggatag ataagaacaa catggatcgt tacccgatac tcgacaacat
     1741 caactcgccg gaggacgtaa aaaaactgcc ggaaggccag cttcccgttc tcgcggcgca
     1801 aattcggcaa tacatgatcg aaaacgtcgc gcgctgcggt gggcatctcg cttccagcct
     1861 cggcgcggtg gagcttacca tagccatgca cagggcgttc tccagcccga cggacgagat
     1921 cgtattcgac gtggggcacc agtcctacgc gcataaaatt ttgaccgggc ggcgggaagc
     1981 tttctgcacg ctccggcagg aaaacggcct cagcggcttt cccaagcgcg aagagagcga
     2041 acacgacccg ttcaacaccg ggcacgcgag cacggcgatt tccgccgctc tcggaatagc
     2101 gcgcgcaaaa cgcctgcgcg ggcaggaagg cgcggcaatc gcgctggttg gcgacggcgc
     2161 gcttactggc ggccttgcgt ttgaaggcct gaacgacgca ggccaggcgg acgtgccgct
     2221 gattgtgatt ctcaacgaca acgaaatgtc gatttctcaa aatgtcggcg cgctgcaccg
     2281 catgctggtg aacatgcgcg taagtcccgg ttacgtgcgt ttcaaacgtt ttctggtgcg
     2341 cgtgctcgat accggcattc tggggcgctg gctttcgcgc catatggaga acctcaaaaa
     2401 ccgcatcaag agctttttaa tgccgaagcg cctctttgaa gatatgggct ttctctacct
     2461 cggtcccatc gacgggcacg atatccccgc gctgacgcgg atgttccgcc gggcaaagga
     2521 actcaaagtt cctgtgatcg tccactgcct gacgcaaaag ggcaaagggt acgcgttctc
     2581 ggagcggaat ccggaaaagt accatggagt ggctccgttc tccatcgaca c
//
LOCUS       DAOMGY010000181         2625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_42840_length_2625_cov_2.820623, whole genome shotgun sequence.
ACCESSION   DAOMGY010000181 DAOMGY010000000
VERSION     DAOMGY010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42840_length_2625_cov_2.820623"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="PLR57_03635"
     CDS             complement(<1..136)
                     /locus_tag="PLR57_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQQ40579.1"
                     /translation="MTQAAGALTEGVYYILLSLNTPMHGYGIMQNVELLSGGRVRLAA
                     G"
     gene            complement(277..969)
                     /locus_tag="PLR57_03640"
     CDS             complement(277..969)
                     /locus_tag="PLR57_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HQQ40580.1"
                     /translation="MKTIGLIGGMSWESTVTYYQLLNEAAKQRLGGLHSAKVLLYSVD
                     FFEVEALMSRGEWDKAADLLGGVAERLERAGADFVLICTNTLHKVAPEVQSRISVPLV
                     HIAEAAAEELVSHGISRVGLLGTKYTMTQAFYRDKLTEQGLEVILPNEDDIELVNRVI
                     FEELCLGIVKPESKAEYLRVITDMQRRGAEAVLLGCTELGLIVSQEEVSLPLFDTTVV
                     HANKAADLALTE"
     gene            complement(1065..1688)
                     /locus_tag="PLR57_03645"
     CDS             complement(1065..1688)
                     /locus_tag="PLR57_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HQQ40581.1"
                     /translation="MPYSLDLKQITVTDYELLLKNQNLLPGRRILLEEIDSRFEAIRR
                     AGINTADRLKKELSTPQKLAAFADKSGIPEGYLVILKRELGSLEQKPVPLSNFPDMDA
                     ALFVKLEERGILNSKDYLERAAGESAELDALCDLVRINGVGAVAARAFYEAGYRSAAD
                     VAYAQAGTMLARVSAVNEQKKYYNAKLGVKDMQFCINFAVLLEKYSK"
     gene            complement(1730..>2625)
                     /locus_tag="PLR57_03650"
     CDS             complement(1730..>2625)
                     /locus_tag="PLR57_03650"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HQQ40582.1"
                     /translation="LSVYSVIEGLLTGNVNILKLPAEGDVLSEWILAELIKLEPRISH
                     KVFTFRLSSDDRASMEKLASLADAIVVWGGDEAVSAVRKLAKPDTRIIEWGHKISFAY
                     VSGDLVSDADLEGLADNICSTNQLLCSSCQGIFVDTEDFKDVSAFSERFFAVLDQRAS
                     ADPLDNDLFRAAQLTLELYTEELEAEGRERKAWRNERCSVIACSDSALIVSHLFRSVW
                     VKPLPRYRILHELKQHKNHLQTAALLCSPEDRVRLEELLITAGVARITRGENMSRSYC
                     GMPHDGEYALRRYVRAVTRED"
BASE COUNT          566 a          781 c          633 g          645 t
ORIGIN      
        1 tccccgccgc gagccgcaca cgcccgccgg atagaagctc cacgttctgc atgatgccgt
       61 acccgtgcat gggcgtgttc agcgaaagaa ggatgtagta tacgccttct gtcaatgcgc
      121 ccgccgcctg tgtcattgca atcactctcc ccctgtattg tatcgtcgta cgttatatcg
      181 gctgtcgtta tatatactat atcggtcgcc gatacatgtc aagcattttt tcgcaaaaaa
      241 aatacgccgc gttcagcggc gtaaaattca cattcgttat tccgtcagcg ccagatcggc
      301 ggctttgttc gcgtggacca cggtggtatc gaacagcggc agcgatactt cctcctgaga
      361 aacgatcaac ccgagttccg tgcagccgag cagaacggct tccgcgccgc gccgctgcat
      421 gtctgtaatc acgcgcagat attccgcttt ggattccggc ttcacgattc caaggcaaag
      481 ttcttcaaag atcacgcggt tgacgagctc gatatcgtct tcgttcggca ggatgacttc
      541 aagcccctgt tcggtcagct tgtcgcggta gaacgcctgc gtcatggtgt atttcgtgcc
      601 gagcagaccg acacgggaaa taccatgcga aaccagctct tccgccgccg cttcggcaat
      661 atgcacgagc ggaacggaaa tgcggctttg cacctccggg gcgaccttat gcagcgtgtt
      721 ggtgcagatg agcacgaaat ccgcgcccgc acgttcgagg cgttccgcca cgcctccgag
      781 caggtcggcg gccttgtccc actccccgcg ggacatcagc gcttccacct cgaaaaagtc
      841 cacgctgtag agcagcacct ttgccgagtg cagcccgcct aggcgctgct tggccgcttc
      901 gttgagcagt tgatagtagg tgaccgtgct ttcccagctc atgccgccga tgagtccgat
      961 tgttttcatt gtgttctcct gtatcgttac attctcttca gctaaattat cgtctattct
     1021 aatttctcaa gacgcttttt gcaaccgaat tatttccgtt ccgtttactt gctgtacttc
     1081 tccagcagaa cggcaaaatt gatgcaaaac tgcatgtcct ttacgccgag ttttgcgttg
     1141 tagtacttct tttgctcatt aacggcgctt acgcgggcaa gcattgttcc cgcttgggcg
     1201 tacgcaacat ccgccgccga acgataccct gcctcataga acgcccgcgc cgccacagcg
     1261 cctacgccgt taatccgcac aaggtcgcac agcgcgtcca gctctgcact ttctcctgcc
     1321 gcgcgttcca ggtagtcctt tgagttcaaa ataccgcgtt cttcaagttt tacaaagagt
     1381 gcggcatcca tatccggaaa gtttgaaagc ggaacgggtt tctgctccag acttccgagt
     1441 tcgcgtttca gaataaccag atacccttcc ggaataccgc tcttatccgc aaatgcagcc
     1501 agcttttgcg gcgttgacag ctcctttttc aggcggtctg ccgtgtttat gcccgcccgc
     1561 cggatagctt caaacctgct gtcgatctcc tccagcagaa tcctgcgtcc gggcagcaga
     1621 ttctggtttt tcaacagcaa ctcatagtcc gtaacggtga tttgtttcag gtcaagagag
     1681 tacggcatat tgaatctcct tactactaat tatcatgtta tactgatcgt cagtcttccc
     1741 gcgtaaccgc ccgtacataa cgacgcagcg catactcacc gtcatgcggc atgccgcagt
     1801 acgatcggga catgttttcc ccgcgtgtga tgcgggcgac ccccgctgta atcagcagtt
     1861 cttccaatct cacccggtct tccgggctgc agagcagcgc cgccgtttgc agatgattct
     1921 tgtgttgctt caattcatgt agaattctgt atctcggcaa gggtttgacc caaaccgaac
     1981 ggaataaatg cgaaactatt agcgcgctgt cagaacaggc aatcaccgaa caacgctcgt
     2041 ttctccaagc ctttcgttcc ctgccctccg cttccagttc ctccgtatag agttccagcg
     2101 tcagttgggc ggcgcggaac agatcattgt ctaacggatc ggcggaagcg cgctgatcaa
     2161 gaaccgcgaa aaaccgttcg gaaaatgcag aaacatcttt aaaatcttcc gtatcgacga
     2221 agattccctg acaggatgag cacaacagct ggttcgtact gcatatgtta tccgcaaggc
     2281 cttccaagtc cgcatctgaa acaagatcgc ctgaaacata cgcaaagctg atcttgtgtc
     2341 cccactcgat aatgcgcgtg tcgggttttg ccagtttgcg tacggcagaa accgcctcgt
     2401 cgccgcccca gacaacaatc gcgtccgcaa gcgacgccag cttttccatc gacgccctgt
     2461 cgtccgacga caggcgaaac gtaaaaacct tgtgcgaaat gcgcggctcc agttttatca
     2521 gctccgccag aatccattcg gaaagcacgt ccccttcggc gggcagtttc agaatgttga
     2581 cgtttccggt taacagtcct tctataacac tatagacgga cagtg
//
LOCUS       DAOMGY010000182         2623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_42885_length_2623_cov_2.970016, whole genome shotgun sequence.
ACCESSION   DAOMGY010000182 DAOMGY010000000
VERSION     DAOMGY010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42885_length_2623_cov_2.970016"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="PLR57_03655"
     CDS             <1..1422
                     /locus_tag="PLR57_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQQ40583.1"
                     /translation="PSVGQSEYEPNDFFGGDCSGIREKLTYLKDLGVTALYLNPVFES
                     ASNHRYDTADYHKIDPIFGTEEEFSALCSEAKELGIRIMLDGIFSHTGADSRYFDKFS
                     RYEDVGAYESESSPYRKWYTFHGDRDHYDCWWGFPTLPNVNELEPGYTAFITGDDGVL
                     NHWARAGAHDWRLDVADELPDEFIRILRKRVKQNDPEGVLLGEVWEDCSNKYGPGGRR
                     GYVNGDELDSAMNYPFTDAAIRFLTGHIDAYALDETLQTQREHYPKPFYEACLNLLSS
                     HDIIRAATALAGAPDRNAVSRKLQAAFRPGEQEKQKGFRRLILATALQMALPGVPCVY
                     YGDEAGMTGMADPFNRGTYPWGTENPETMAAFEALMHARRDTLALKRGLCRMGALSPE
                     VFALIRYLPEQGQTALLMLNRSEREQSVSFCPEWLQQGPDGETQVSLPGSMTDVLTGD
                     AIHQENGECKTVLPPMTARLWIG"
     gene            1483..1941
                     /locus_tag="PLR57_03660"
     CDS             1483..1941
                     /locus_tag="PLR57_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQQ40584.1"
                     /translation="MFQRQQKYLDDLNRALLIAALVLSIIGLFFFKFGTWPRYVVTGL
                     AAALLILLVLRMFSKDTSKRYRENMKYLTAVTAISNWFKRTFSKSASGGSTVKTHRAH
                     KARKNPTWNEIKQYKYLICPQCTQRLRVPRGKGRIRVTCTRCGNVFETKS"
     gene            2089..2289
                     /locus_tag="PLR57_03665"
     CDS             2089..2289
                     /locus_tag="PLR57_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HQQ40585.1"
                     /translation="MQATKEMLIQQVIELDEGTAEILMNSGMHCLGCAMAHGESIEQA
                     CAVHGIDVDALIGEINAYLAAK"
     gene            2478..>2623
                     /locus_tag="PLR57_03670"
     CDS             2478..>2623
                     /locus_tag="PLR57_03670"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HQQ40586.1"
                     /translation="MFGYIRPLECELKVREQAEYRAFYCGLCKTIGRRYGTLERLTLS
                     YDCA"
BASE COUNT          613 a          710 c          799 g          501 t
ORIGIN      
        1 ccctcggtgg gacagagcga gtacgaaccg aacgactttt tcggcgggga ctgcagcggc
       61 atccgtgaaa agctgacgta tcttaaagac ctcggcgtga cggcgctcta cctcaacccg
      121 gtctttgaat ccgccagcaa tcaccgctac gatacggcgg actaccacaa gatagacccg
      181 atcttcggaa ccgaagagga gttttccgcc ctctgcagcg aggcaaagga actcgggatc
      241 cgaatcatgc tggacggcat cttttcccac acaggcgcgg acagccggta tttcgacaaa
      301 ttttcgcggt atgaggacgt aggcgcgtat gagagcgaat cttcgcccta tcgcaaatgg
      361 tacacgttcc atggcgaccg cgaccattac gactgctggt gggggttccc aacgctgccg
      421 aacgtcaatg agctcgaacc gggctacacg gcgtttatta cgggcgacga cggcgttttg
      481 aatcactggg cgcgcgcggg cgcgcatgac tggcggctgg acgttgcgga cgaactgccg
      541 gacgagttta tccgcattct ccgaaagcgc gtcaagcaga acgacccgga aggggttctg
      601 ctcggcgaag tctgggagga ctgctccaat aagtacggcc cgggaggcag gcgtggatac
      661 gtcaacggcg acgagctgga ttccgcaatg aactatccgt tcacggacgc ggcgatccgg
      721 tttttgaccg ggcatatcga cgcttacgcg ctcgatgaaa cgctgcagac gcagcgcgag
      781 cattacccga aaccgtttta cgaggcatgc ctcaacctgc tctcctcgca cgatatcatc
      841 cgcgcggcaa ccgcgctggc gggcgcgccg gaccgaaacg ccgtttcccg caagctgcag
      901 gcagcgttcc gcccgggtga acaggagaag cagaagggct ttcgcaggct gattctcgca
      961 accgcgctgc agatggcgct gccgggcgtt ccgtgcgtat attacggcga cgaagcgggc
     1021 atgaccggca tggccgaccc cttcaatcgc ggaacgtacc cctggggcac ggaaaacccc
     1081 gagaccatgg cggcattcga ggcgctgatg catgccagaa gggacactct cgcgctcaag
     1141 cgggggcttt gccggatggg ggcgctctcg ccggaggtat tcgcgctgat tcgatatttg
     1201 ccggagcagg gacagaccgc gctgttgatg ctcaaccgtt cggagcgtga gcagagcgtg
     1261 agtttctgcc ccgagtggct ccagcagggg ccggacggcg aaacgcaggt ttcgctgccg
     1321 gggagcatga cggacgtgct gacgggcgac gcgattcacc aggaaaacgg agaatgcaaa
     1381 acagtcctgc cgccgatgac cgcgcggctc tggattgggt gacctgccat ttggcaaaca
     1441 ggataccggc cgtgaaccgg caaaagaaaa cgagggattc atatgtttca acggcaacaa
     1501 aagtatctgg acgatctcaa ccgcgcgctt ctcattgcgg cgctggtgtt gtcgattatc
     1561 gggctgttct tctttaagtt cggcacttgg ccgcgctatg tggtaacggg tcttgcggcg
     1621 gcgctgctga ttctgctcgt gcttagaatg ttcagcaagg atacgtccaa gcggtatcgt
     1681 gagaatatga agtatttgac cgccgtgacc gcgattagca actggtttaa gcggacgttt
     1741 tcgaaaagcg cttccggcgg ttccacagtc aaaacgcatc gcgcgcacaa agcgcggaag
     1801 aacccgacct ggaacgagat caagcagtat aagtatctga tctgtccgca gtgcacccag
     1861 cgcctccgcg ttccgcgcgg caaaggccgc atccgcgtga cctgcacgcg atgcggaaac
     1921 gtgttcgaaa ccaagagctg aacagaaaca gccggagaaa cgcgccaaaa gtgaatttgc
     1981 ggcaaactgc gcgggaaaac tattgcattt ttacaaagtg tgatatacta gtaaaacagt
     2041 agtgattata ctgaactcaa gtattgaaat attcggaggg aacagactat gcaggcaacc
     2101 aaagagatgc ttattcagca ggtgatcgag ctggacgaag gcaccgcgga gattctaatg
     2161 aactcgggca tgcattgcct cggctgcgcg atggcgcacg gcgagtcgat tgagcaggct
     2221 tgcgcggtgc acggcatcga cgttgacgcg ctgattggcg aaatcaacgc gtatctggcg
     2281 gcgaaataac cgccggcaaa acgaacccga aagcggccgt ccgtcgtgac ggctgttttt
     2341 ctgtccgttg attgtaagga aaaaccgccc cgttcctcac tatttgacac aatcgtttac
     2401 aagaacgggg caaaataaaa tggaatcacg cggcgttcca tgataaaata ccatggaaag
     2461 cgcgaaacgg aggacaaatg ttcggctata tccggccgtt ggaatgcgaa ttgaaggtgc
     2521 gcgagcaggc ggaataccgc gcgttttact gcggcctctg caagaccatc ggcaggcgat
     2581 atggaacttt ggaacggctg acgttgagtt acgactgcgc gtt
//
LOCUS       DAOMGY010000183         2622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_42908_length_2622_cov_3.485002, whole genome shotgun sequence.
ACCESSION   DAOMGY010000183 DAOMGY010000000
VERSION     DAOMGY010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42908_length_2622_cov_3.485002"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="PLR57_03675"
     CDS             <1..1641
                     /locus_tag="PLR57_03675"
                     /inference="COORDINATES: protein motif:HMM:NF039328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6034 family protein"
                     /protein_id="HQQ40587.1"
                     /translation="EAKAETGVRANIRMCCQYAVGAPAMVFPNLLFLPLGALVSMSDS
                     EADHALRHELTHFRRGDPAVSVLLAALCAVYWFNPVVWAAAYLMRADMEAACDAAVTK
                     RFSRDEKERYASLLLALYSLPALGAPALGLSGVGVAHQAERRVRGVFEGRPSTLPVRA
                     AALLLALILAFGCFTTACQPLTGTSGHVENPDNPWISDVPVKVLEHVKLEPEALQENV
                     VFTVDADVTQPTAYEPVTVGVARRTIDFAEFQLMIAAVMPEVAWTSSQNEDGTEITAT
                     GERNGEAYTASCYTYKDGTVSFAVYPENLYIIREGFLVNDIEMEQDYFAVLHQSIQTT
                     ASEAQPKADAVVNALGAEGLMLQSAERACRFDTNLPGNVVLSSGWSFVYVPDCGGLPA
                     NYRLGGSGIDDPFGDGYYGVPMKPVLSVYVDETGVSYIDWRSPFTVTQTNAQTEPLLD
                     YEEAIAGAKNILKERYEGRELTTQLTFTVTAIQLASIIVSDERMSGSAAFDYSGDTGR
                     LVPVWEIVCVESNPEGSYWRSIVLPFTAIDGVALTQQE"
     regulatory      1715..1916
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2064..>2622
                     /locus_tag="PLR57_03680"
     CDS             2064..>2622
                     /locus_tag="PLR57_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HQQ40588.1"
                     /translation="MKHKVNLKKRLALLLALLTALSALSGCAAAKPAGAEPAAVPAEA
                     AEQTAEPAAEPITLTDMAGREISLESPATRVVALTAADCEILYAIGAGETLVGRGEYC
                     DYPAEVSAVPSVQSGMETNLEQIIALQPQVLIMSTMAQTEEQVASLEAAGIKVVVSDA
                     QDIEGVYTAIALIGALTSKNAEAQGV"
BASE COUNT          562 a          706 c          828 g          526 t
ORIGIN      
        1 gaagccaagg cggagactgg cgtgcgcgca aacatccgca tgtgctgtca atacgccgtc
       61 ggtgcacctg ctatggtgtt tcccaacctg ctgtttttgc cgctcggcgc gctcgtttcc
      121 atgagcgaca gtgaggcgga ccatgccctg cggcacgaac tgacgcattt tcggcgcggc
      181 gatcccgccg tcagcgtact gcttgcggcg ctctgcgcgg tgtactggtt caatcccgtc
      241 gtgtgggcgg ctgcgtatct catgcgggcg gatatggaag cagcctgcga cgcggcggtg
      301 acaaagcgat tttcgcggga cgaaaaagag cggtatgcga gcctgctgct tgcgctgtac
      361 tcgctccccg cgctcggcgc gcccgcgttg gggctgtcgg gggtaggcgt tgcgcatcag
      421 gcggaacggc gcgtgcgcgg cgtgttcgaa ggcagaccct ccactttgcc ggtgagagcg
      481 gcggcgctgc tgcttgcgct cattctggcg ttcgggtgct ttacgaccgc ctgccagccg
      541 ttgaccggca caagcggaca tgtggaaaat cctgacaatc cctggatttc ggacgttccg
      601 gtcaaggtgc tggagcatgt gaaacttgag ccggaagcgc tgcaggagaa tgttgtcttt
      661 actgtggacg cggatgtgac gcagccaacg gcgtatgagc ctgtcacggt tggcgtggct
      721 cgccggacga tcgactttgc ggagtttcaa ttaatgatcg cggcggtgat gccggaggtt
      781 gcctggacga gttcacaaaa cgaggacggc accgaaataa ccgcaaccgg cgaaaggaac
      841 ggagaggctt acacggcttc ttgttataca tacaaggacg gaaccgtcag cttcgccgtt
      901 tatcctgaga atctctatat aatccgtgaa ggattcttgg taaacgatat tgagatggaa
      961 caggactatt tcgctgtgct tcaccagtcc atccaaacca cagcctccga ggcgcagcca
     1021 aaggcggacg cggtggtaaa cgcgcttggc gcagaaggtc tgatgctgca aagcgcggag
     1081 cgcgcctgcc ggtttgatac gaacctgccg ggaaatgttg ttctctcaag cggatggagc
     1141 ttcgtgtatg tgcctgattg cggaggcctt ccggcaaatt atcgcttggg tggatccggt
     1201 atcgacgatc cgtttggcga cgggtattac ggcgtgccga tgaagcccgt gctcagcgtg
     1261 tatgtggatg aaaccggtgt gtcgtatatc gactggcgca gtccgtttac tgttacgcag
     1321 acgaacgcgc agacggaacc gctgttggat tatgaagaag ccatcgccgg agcaaagaat
     1381 attctaaagg agcgttacga agggcgggag ctgaccacgc agttgacgtt caccgtgacg
     1441 gcaattcagc ttgcctccat tatcgtcagc gacgaaagaa tgagcggatc ggcggcattc
     1501 gactactccg gcgatacggg aaggcttgtg cccgtctggg agatcgtctg cgtggaaagc
     1561 aatccggagg gaagctattg gcgttcgatt gttctgccgt ttacggccat cgacggcgta
     1621 gcgctcacgc agcaggagta gcagaagaaa atacttctcc aacgcggttg acgcggaggg
     1681 gaagaagagg atataattcg gttaaatgaa tagcgtgcga ttctgcgccg gagatggcgc
     1741 cggcgcatcc ggaaacggaa acaatgcgcc gagggaatac ggatgaaagt tccgagctac
     1801 cccagtaacc gtgaagccga cgaacggaca catatgagcc actgcttttt cagcgggaag
     1861 gcgtccaagt aggatgaagc caagccggga gacctgttac cgcatgatat gctcctgggg
     1921 tggagtgaag ctatgcggcg gaagaaaaac gcaggtattt tttcattgct cctctcacaa
     1981 gagtggagca atttttgtta tgctccttcc tctctattca ttacgtacgc tcaaagagat
     2041 ttgaaaggag tccccaaaaa gaaatgaaac acaaagtgaa cctcaaaaaa cgtctcgcac
     2101 tgctgcttgc gctgctgacg gcgctttccg cgctctccgg ctgcgcggca gccaagcccg
     2161 ccggtgcgga accggccgcc gtccctgcgg aagccgccga gcaaaccgcc gaacccgcgg
     2221 cggaacccat tacgcttacg gatatggccg ggcgtgaaat ctcgctggaa tcgcccgcca
     2281 ctcgcgtggt agcgcttacg gcggcggact gcgaaatcct ctacgccatc ggcgcgggag
     2341 aaacgctcgt cggccgcggc gagtactgcg actatcccgc ggaagtcagc gccgtaccct
     2401 ccgtgcagtc cggcatggaa accaacctcg agcagattat cgcccttcag ccgcaggtgc
     2461 tgattatgag cacgatggcg cagacagagg agcaggttgc ttctctggaa gcggcgggca
     2521 tcaaggttgt cgtttccgac gcgcaggata tcgagggcgt gtataccgcc attgcgctca
     2581 tcggcgcgct gacgagcaaa aacgccgaag cgcagggcgt ga
//
LOCUS       DAOMGY010000184         2616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_43079_length_2616_cov_2.775088, whole genome shotgun sequence.
ACCESSION   DAOMGY010000184 DAOMGY010000000
VERSION     DAOMGY010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43079_length_2616_cov_2.775088"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1329)
                     /locus_tag="PLR57_03685"
     CDS             complement(220..1329)
                     /locus_tag="PLR57_03685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQQ40589.1"
                     /translation="MDKKKLNRIFLLSAAGVFLLLGVVGAVRSLRPETSADPIVVFTQ
                     PEAEVISVEAAEVTPSPSPVPITYEGTVILLVDRVPVMTLASELEAKRMLWEYLSVSA
                     IAPEGERFVSARFDCELILTQGDSYTKPLESFQALAMLQGNPAIVPIEVVAQRIAYAE
                     SSPEVQESGESALPKGFRIVTQLGTGTLTQTVTEVTYRAGEAVQTGTPLTKTLTEARA
                     SILRTGAYTKKDTSGTAQRLEGPEGKDAGDLKLTYPMRGQVVKFFGYNEGVMNNGLDI
                     SNKAGTDVTAPGEGLIVYCGERGAYGFVVDIDHGNGFVSRLTHLSNVQVEMNQRVFAG
                     DLIGSLAEDEDGGKPVFHYELIIDGIPYNPLYYID"
     gene            complement(1396..>2616)
                     /locus_tag="PLR57_03690"
     CDS             complement(1396..>2616)
                     /locus_tag="PLR57_03690"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQQ40590.1"
                     /translation="TAATSASTGLVKNGTVNLRAKASTSASVVEQLAVSTPLTLLGMT
                     GEWYYVSAPSGNTGYIRYDYVLITGSSMIARGVVSGSAVNYRTGPGTSYSSIGQLSLD
                     TQLGIYGMVDGWYKVKAMTTGTDGFVSKSYVTITAATAENLTPATPTPIGSTTATPTA
                     VPTPTVPGATPTPVVIGGATPTPTPMATPSTTLAPEYAAAGVLNAAGVNIRAGASTST
                     KSYGKLMKNTSVGIYEKKGSWYRVRILATGLDGYVYAKYITITQTASSTSSSTTCSSP
                     GYINASGVYVREGASTRYSRLGKFAKYTSVKVIASSGSWYQIEVPSADITGYVLAKYV
                     TLTSTTQQETQTGVITSRLNLRAQPSTSSGSKVLLTMSKGATVTVYSTSNGWCYVNYN
                     GTMGYCVASCVKIG"
BASE COUNT          512 a          773 c          766 g          565 t
ORIGIN      
        1 aagtgtgtgg acaacgccat tcaacgggtg aaaaagaagc tggaagagta cctgaaaaac
       61 gcgggataaa cacaaaataa aaacggcggc tcgaacgagc cgctgttttt tatgatatta
      121 tccgggggct ctgccccctt ttctcgtttt ttaccggggg cttcgccccc gtacccccga
      181 ccaaagggca cgccctttgg aaacccgtat tttatcaatt tagtcgatat aatacagcgg
      241 attatacgga attccgtcga taattagttc atagtggaag accggcttgc cgccgtcctc
      301 atcctccgcc agcgaaccga ttaaatcccc cgcgaacacg cgctggttca tttcgacctg
      361 cacgttgctt aagtgcgtca ggcgggaaac aaacccgttg ccgtggtcga tatcgaccac
      421 aaacccgtaa gcgccgcgtt cgccgcagta gacgattagt ccttcgccgg gcgcggtaac
      481 gtccgttccg gctttgttgg agatgtcgag gccgttgttc ataacgcctt cgttatagcc
      541 aaagaacttg actacctgcc cgcgcatcgg gtaggtgagc ttcaagtctc ccgcgtcctt
      601 gccttccggg ccttccagcc gctgggcggt gccggaagta tctttcttgg tatacgcgcc
      661 ggtacgcaag atgctggcgc gcgcttcggt gagcgtcttt gtcagcggcg tgcccgtttg
      721 aacagcttct cccgcgcggt aggtaacctc cgtcacggtc tgcgtgagcg tccctgtacc
      781 aagctgcgtt acaatgcgaa agcccttggg aagcgcgctt tcgccggact cctgcacctc
      841 cgggctcgac tcggcgtatg cgatacgctg ggctacaacc tcaatgggca caatcgcggg
      901 gttgccttgg agcatggcaa gcgcctgaaa cgactccagc ggctttgtgt aggaatcgcc
      961 ctgtgttaaa atcagttcgc agtcgaaccg ggcggaaacg aagcgttccc cttcgggcgc
     1021 gatcgctgag acggaaagat attcccaaag catacgcttt gcttccagtt cggaagccag
     1081 cgtcatcacc ggcacgcggt caaccagcag tatcaccgtg ccttcgtagg tgatcggcac
     1141 cggcgagggc gaaggcgtaa cttccgcggc ttccacagag atcacttccg cttccggctg
     1201 ggtaaagacc acgatcgggt ctgcggacgt ttccggccta agcgagcgca ccgcgccaac
     1261 gaccccgagc aggagaaaaa cgcccgcggc cgagagcaga aagatgcggt tgagcttctt
     1321 tttgtccatt gtacgattga tgccgtccta tatcgaaccc gcctctccgc tacgcggcgg
     1381 acaatcggat tcgctctatc ctatcttgac gcaggaagcg acgcagtaac ccatcgttcc
     1441 gttgtagttg acataacacc agccgtttga cgtcgaatag acggtaacgg ttgcgccttt
     1501 gctcatggtg agcagcactt tcgaacccga actggtcgag ggctgcgcgc ggaggttcag
     1561 ccgcgaggtg attacgcccg tctgcgtctc ctgctgtgtg gtgctcgtaa gcgttacata
     1621 ctttgccagc acatagccgg tgatatccgc ggaaggaacc tcaatttgat accagctgcc
     1681 ggaggacgca atcaccttaa cgctcgtata ctttgcgaac ttgccaaggc ggctgtagcg
     1741 cgtgctcgcg ccctcgcgga catacacgcc ggaagcgttg atgtaccccg ggctcgagca
     1801 agtcgtgctg ctgcttgtgc tggaagcggt ctgcgtaatg gtgatgtact tggcatacac
     1861 atatccgtcc aaaccggtag cgaggatgcg gacgcggtac caggagccct tcttttcata
     1921 aatgccgacg gaagtgttct tcatgagctt accgtagctc ttggtggagg tgctcgctcc
     1981 cgcgcggata ttgacgcccg ccgcgttgag cacgcccgcc gccgcatact ccggcgcaag
     2041 cgtcgtgctc ggcgttgcca tcggggtagg cgtaggagtt gctcctccaa tcaccacggg
     2101 ggtcggcgtt gcgcccggaa ccgtgggcgt tggaacagcg gtcggcgtcg cggtcgtgga
     2161 accaatcgga gtcggcgttg cgggagtcag gttctcggcg gtcgccgccg taatcgttac
     2221 atagctcttg ctgacgaatc cgtccgtacc ggtcgtcatg gccttgacct tataccagcc
     2281 gtccaccatg ccgtagatgc cgagctgggt atccagcgaa agctgtccga tggaagaata
     2341 gctcgtgccg gggcccgtgc ggtagttcac ggcgctgccg ctgaccaccc cgcgtgcaat
     2401 catgctgctg ccggtgatga gcacatagtc atagcggata tagccggtgt tgccgcttgg
     2461 cgcggaaaca tagtaccact cgcccgtcat gccgagcagc gtcagcggcg tgctcacagc
     2521 aagctgttca accacgctcg cgctcgtgga agcctttgcg cgcaggttga ccgtgccgtt
     2581 tttgaccaga cctgtggaag cgctggtagc cgcggt
//
LOCUS       DAOMGY010000185         2612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_43156_length_2612_cov_4.292139, whole genome shotgun sequence.
ACCESSION   DAOMGY010000185 DAOMGY010000000
VERSION     DAOMGY010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43156_length_2612_cov_4.292139"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..616)
                     /locus_tag="PLR57_03695"
     CDS             complement(29..616)
                     /locus_tag="PLR57_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40591.1"
                     /translation="MDIRFDNEPKKGQTPTTRLHMPNKETAEAFESRSERKPSRGRSA
                     SRAAEEPHVRVEKPELNPLARIGLILCGFIFAGMVLFTLTGYERISRAYADINALNTE
                     IDSTKLRINELEVQIECAVTIQDAQKVAEAYGMQYPEKSQYVKIGDPLPFSGSDYPVE
                     TTTEPTESPAASTPDPGTGDTGDTTGGGSQPPSGG"
     gene            complement(694..1635)
                     /gene="rsmH"
                     /locus_tag="PLR57_03700"
     CDS             complement(694..1635)
                     /gene="rsmH"
                     /locus_tag="PLR57_03700"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011949033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQQ40592.1"
                     /translation="MSGEFKHIPVLLDETLTLLAPERGGTFVDGTLGGGGHAEAVLAR
                     LPQSGKLIGIDRDWEAVRAAEERLTAYGERFTALHGNFFEMKQLLAEADVTEVNGILL
                     DLGVSSYQLDTQERGFSYKIDAPLDMRMDQGAPLSAYDVVNGYAPEEIARVLWEYGEE
                     RFSRRIADFIARARAERPIETTVELADIVRQAIPAKFRNEPQHPARRTFQAIRIEVNS
                     ELRGLDRAVESACDLLQKGGRMCIITFHSLEDRIVKQAFRRFENPCTCPPSAPICICG
                     KQPKAKILTNKPLTCSDREAERNPRSTSAKLRCIQKI"
     gene            complement(1632..2078)
                     /gene="mraZ"
                     /locus_tag="PLR57_03705"
     CDS             complement(1632..2078)
                     /gene="mraZ"
                     /locus_tag="PLR57_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQQ40593.1"
                     /translation="MLIGSFEHMLDAKGRVFIPAKWREDVGDTLIVTLGLLESPSQRC
                     LSGMSVAEWEKLSEKFAKLPMTDTRGQQIRRKLYSLAAACEIDKQGRILIPAQLRELA
                     GLTKDATMLGVDNHVEIWNPQSLQEYNVACEESYSDSMAHLSEVGV"
     gene            2476..>2612
                     /locus_tag="PLR57_03710"
     CDS             2476..>2612
                     /locus_tag="PLR57_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40594.1"
                     /translation="MASDYTVDKIKIMEGLDAVRMRPGMYIGSTGQRGLHHLLWEIID
                     NS"
BASE COUNT          535 a          770 c          683 g          624 t
ORIGIN      
        1 ccatttgagg aacgtccttt cgttaggatc aaccgccaga aggcggctgg ctccccccac
       61 ccgtcgtatc tcccgtatcg cccgtccccg gatcgggcgt cgaagccgcc ggggattccg
      121 taggctccgt ggtcgtttcc accggataat cgctgccgga gaacggcagc ggatcaccga
      181 tttttacata ctgtgatttt tcaggatact gcatgccgta agcttcggcc actttctgcg
      241 catcctgaat ggtaacggcg cattcaatct gcacttccag ctcgttgatg cgcagctttg
      301 tagaatcaat ctccgtattc agggcgttga tatccgcata cgcacgcgaa atgcgctcgt
      361 agcccgtcag cgtaaagagc accatgcctg caaagatgaa tccgcagagg atcaggccga
      421 ttcgcgcaag cgggttaagc tccggctttt caacgcgtac atgcggttct tccgccgcgc
      481 gggacgcgct tctgccgcgg ctcggctttc tctcggaacg gctctcaaaa gcttccgcgg
      541 tttccttgtt tggcatatgc agccgcgtgg tgggcgtttg ccctttcttt ggctcattgt
      601 caaagcgaat gtccaaattt cgatctcctc tattctagcc ccgccgccgt caaagagcaa
      661 gacttgacgg cgaaacatgc cacaagacgg gcgctagatc ttctggatac accgaagttt
      721 cgcgctcgtc gaacgggggt tgcgttccgc ctcccgatcc gagcaggtca gcggtttatt
      781 cgtgaggatc ttcgcctttg gctgcttgcc gcagatgcag atcggtgcgg acggcgggca
      841 ggtgcaggga ttctcaaacc ggcggaacgc ctgcttgaca attcgatctt ccaacgaatg
      901 aaatgttatt atacacattc ttccgccttt ttgcaacaga tcgcaggcgg actcgaccgc
      961 gcggtccagc ccgcgcagtt cgctgttgac ttcaatacga atcgcctgga acgtacgccg
     1021 cgcgggatgc tgcggctcgt tgcggaattt cgcgggaatt gcctgacgga cgatatccgc
     1081 cagctccaca gtggtttcaa tgggacgctc cgcacgcgca cgggctataa agtccgcaat
     1141 gcggcggctg aagcgctcct cgccgtactc ccagagcacg cgggcgattt cttccggcgc
     1201 gtagccgttg accacgtcgt aagcggagag cggcgcgccc tgatccattc gcatatcgag
     1261 aggagcgtct attttatagg aaaaacctct ctcctgcgta tccagctggt aggaggaaac
     1321 gccaagatca agcagtatgc cgttgacttc cgtaacatcc gcttccgcca gcagctgctt
     1381 catttcaaaa aagttaccgt gcagcgcggt aaaacgttcg ccgtaagcgg tcagtcgttc
     1441 ctccgccgct cgcacggctt cccagtccct gtcgattcca atcagcttgc cgctctgcgg
     1501 cagccgcgcc agcaccgcct ccgcgtgacc gcctccgccg agggtgccgt ccacaaacgt
     1561 acctccgcgc tccggggcga gcagcgtcag cgtctcgtcc agcagcacgg ggatatgctt
     1621 aaattctccg ctcatacgcc aacctccgaa agatgcgcca tggaatcgct gtagctttcc
     1681 tcgcaggcaa cattgtattc ctgcagggac tgcggattcc aaatttccac atggttgtcg
     1741 acgccgagca tcgttgcgtc cttcgtcagc cccgcaagtt cgcgcagctg cgcgggaatc
     1801 aggatgcgcc cctgcttatc gatttcgcag gccgccgcaa gggaatacag tttccggcgg
     1861 atctgttgtc cgcgcgtatc cgtcatgggc agtttggcaa atttttcgga gagcttttcc
     1921 cattcggcaa cggacatgcc ggaaaggcac cgctgggaag gcgattcgag cagccccagg
     1981 gtaacgatca gcgtgtcgcc gacgtcttct ctccacttag cgggaatgaa cacgcgccct
     2041 tttgcgtcca gcatatgctc aaacgaacca atcagcacgt gtctgtccct cctgttgtgc
     2101 aaagtgttga ccggtataaa aggatagaga accggtcagt ttaggcaaag tatacaccac
     2161 tttcccccat ttgtcaccac ttttcacgaa attagcacca ttttttcgcc gttcatttta
     2221 cgtttgctcc gccttctctt tgccgcaatt gccgctataa cgctgcttta cgggaaaaga
     2281 gattggtagt gcatacaaag tatggtatac ttagttgttg tagacgctag atatggaagt
     2341 tcattccgct atcaatccgt tatcaacctg ttttcatccg taaccctctc gtgagagatt
     2401 taaaatgatc tccggtattt tttcgctttc acggtattta tttgaattcc caaaattcaa
     2461 gcatcaggag tttgtatggc atcggactac acggtagata aaatcaaaat catggagggg
     2521 ctggacgcgg ttcgcatgcg tccgggcatg tacatcggct ccacgggaca gcgcggcctg
     2581 catcatctcc tctgggaaat catcgacaac tc
//
LOCUS       DAOMGY010000186         2606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_43319_length_2606_cov_2.938848, whole genome shotgun sequence.
ACCESSION   DAOMGY010000186 DAOMGY010000000
VERSION     DAOMGY010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43319_length_2606_cov_2.938848"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PLR57_03715"
     CDS             complement(<1..1175)
                     /locus_tag="PLR57_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQQ40595.1"
                     /translation="MQMIKKLSAFIGKYKVYAILSPVLVFFEVVLEVFIPLLMGRIVD
                     VGIPNNDYAYIFKMGGLMVVMAVGALFFGALCARYAVVASNGLATNLRKGLFEKIESF
                     SFKNTDRFTTPSLITRLTNDVNNIQQSFQMIIRMLARAPFMLILATVMAIRISTRLSL
                     VILCAIPVLSIAMAILAKIAFPRFEAMLKKYDLMNASTQENLVAIRVVKAFVRGKHES
                     VKFADAAAALKNAQRKAEKVLVMAMPIMQITMYLCILLVLWIGGMDILGGNLMTGELM
                     SFLSYITQILMSLMMITMVFVMLVLSSASAKRIIEVLDETPAINDDGANAELTVNNGD
                     VEFKDVCFSYADDPENLALSHIDLRFRSGETIGIIGGSGSAKSTLVQLIPRLYDVLSG
                     "
     gene            complement(1224..1781)
                     /locus_tag="PLR57_03720"
     CDS             complement(1224..1781)
                     /locus_tag="PLR57_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HQQ40596.1"
                     /translation="MDTHSLSKRMMLLQMLRRRYLHGSCFSKEIVPGQYPLLNHLSSN
                     PEATQQELATMLFVSPASVAQSTKRLQKAGLLEKETDPQNLRKNRLRVTEAGQEAVVS
                     VRAVIDALDDRILAGFSQEELASLNGMLERMIINLANEEDLALLRAMEEHSAPPGILF
                     NGHNCPNDPHSCHQRAYPAARPDKP"
     gene            2078..>2606
                     /locus_tag="PLR57_03725"
     CDS             2078..>2606
                     /locus_tag="PLR57_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQQ40597.1"
                     /translation="MKREIHGNTAGIRDIILNEMLGLYDLTMSADEFLSDEVTDQLVR
                     FTSLINREVLVYVSRAGAIEDVRIGDDHSVHMEEMRLVRNIDRLSGVRCIHTHPNETG
                     YLSDVDIGSLNALRLDAMCAIGVKEGRASSVYAAFIGDMEGEERIPVWYGPMRAGRLP
                     QKQLMDAILEADKRLL"
BASE COUNT          603 a          679 c          758 g          566 t
ORIGIN      
        1 ccggagagga cgtcgtagag ccgcggaatc agctgcacca gcgtggattt tgccgaaccg
       61 gaaccgccga taatgccgat ggtttcgccg gagcggaagc gcaggtcgat atgcgacagc
      121 gcgaggtttt ccggatcgtc cgcatagctg aagcacacgt ccttaaactc aacgtcgccg
      181 ttgttcaccg taagttcggc gttggcgccg tcgtcgttga tggcgggcgt ttcatccagc
      241 acttcgatga tgcgcttggc ggacgcgctg gagagaacca gcataacgaa caccatcgta
      301 atcatcatca gcgacatcag aatctgcgta atgtagctca aaaagctcat cagttcgccc
      361 gtcataaggt tgccgccgag gatatccatg ccgccgatcc agagtacgag tagaatgcag
      421 agatacatcg taatctgcat gatgggcatt gccataacga gcactttttc cgccttgcgc
      481 tgggcgtttt tgagggcggc ggcggcgtcc gcaaacttga cgctttcgtg cttgccgcgc
      541 acaaacgctt tgaccacgcg gattgccacg aggttttcct gcgtgctggc gttcatgagg
      601 tcgtattttt tcagcatcgc ttcaaaccgc gggaacgcga ttttggcgag aatcgccatg
      661 gcgatggaga gcacggggat ggcgcagagg atgacgaggg aaagccgcgt gctgatgcga
      721 attgccatga ccgttgccag aatcagcatg aacggcgcgc gcgccagcat gcggataatc
      781 atctggaacg attgctgaat gttgttgacg tcgttggtca gccgggtaat cagcgagggc
      841 gtcgtaaacc ggtcggtatt cttaaaggaa aacgactcga tcttttcgaa cagtcctttg
      901 cgcaggtttg ttgcgaggcc gttggaagcg accaccgcgt accttgcgca gagcgcgccg
      961 aagaacagcg cgccgacggc cataacgacc atcagtccgc ccatcttgaa gatgtaggcg
     1021 taatcgttgt tgggaatgcc aacgtccacg atgcgcccca tcagcagggg gataaacacc
     1081 tcaagcacca cctcgaagaa tacgagaacg ggcgagagga tggcgtaaac cttgtacttg
     1141 ccgataaacg ccgagagttt cttgatcatc tgcatgtgcg ttacttcatc ctttatcttt
     1201 aatcttgttg attgcgaatt tgattacggc ttgtccggtc ttgccgcggg gtatgcgcgt
     1261 tggtggcacg agtgcggatc gttcggacag ttgtggccgt tgaacagtat ccccggaggg
     1321 gcggaatgct cttccatagc gcgcagaaga gcgagatctt cctcattggc gaggttgatg
     1381 atcatgcgct ccagcatgcc gttcaggctc gcaagctcct cttggctgaa ccctgccagg
     1441 atacggtcgt cgagcgcgtc aatgaccgcg cgcacagaaa caacggcttc ctgacccgct
     1501 tcggtgacgc ggagacgatt cttgcggaga ttttgcggat cggtctcctt ttcgagtaaa
     1561 cccgctttct gcagacgctt ggtggactgc gccaccgaag cgggggagac gaacagcatg
     1621 gtagcgagct cctgctgggt cgcctccggg ttgctgctca agtggtttaa aagcggatac
     1681 tgaccgggga cgatctcctt tgaaaagcac gaaccatgca gatacctgcg gcgcagcatc
     1741 tgcaaaagca tcatgcgctt ggaaagcgaa tgggtatcca tactacaaac ctccgagaca
     1801 tactacggtt ccaacatgaa cggaacataa acttttcggg ttaatttcct aaattacaca
     1861 atgttccgca gaactgttaa ccggttaaca gttaagtggc tatcaatata acagacgcgg
     1921 ttttcattgt caatacacgc tttggatggt atgatgatgc caaacgaaaa aacgccggaa
     1981 ttccggcgca aatcgaattg tgaagaaatg gaaacatccg aaaagcgcgg taaatacgtt
     2041 tgttttgacc gcgccgcaaa cggagattaa ggggtatatg aaaagagaga tacacggaaa
     2101 caccgccggc attcgggata tcattctgaa cgaaatgctg gggctgtacg atttgaccat
     2161 gtccgcggac gagttcctct cggacgaagt aacagaccag ctggtgcgct ttacatcgct
     2221 gatcaaccgc gaggtgctgg tttacgtttc gcgcgcgggc gccattgagg acgttcgcat
     2281 tggagacgat cactccgtcc atatggagga gatgcgcctt gtgcgcaaca tcgatcgcct
     2341 cagcggcgtt cgctgcatcc atacgcaccc caacgaaacg gggtaccttt cggatgtgga
     2401 catcggctcg ctcaacgcgc tccggctcga cgccatgtgc gccatcggcg ttaaggaagg
     2461 cagagccagc agcgtctatg ccgcgtttat cggggatatg gaaggcgagg agcgcattcc
     2521 cgtttggtac ggccccatgc gcgcgggacg actgccgcaa aagcagctca tggacgcgat
     2581 tctggaggcg gacaagcggc tcttaa
//
LOCUS       DAOMGY010000187         2600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_43466_length_2600_cov_3.369352, whole genome shotgun sequence.
ACCESSION   DAOMGY010000187 DAOMGY010000000
VERSION     DAOMGY010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43466_length_2600_cov_3.369352"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="PLR57_03730"
     CDS             complement(<1..174)
                     /locus_tag="PLR57_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQQ40598.1"
                     /translation="MAGTVTFDRNRCKGCELCVLVCPKHIIVIDSATNAKGYHPAIVE
                     RMSECTGCASCARM"
     gene            complement(187..870)
                     /locus_tag="PLR57_03735"
     CDS             complement(187..870)
                     /locus_tag="PLR57_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007391663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40599.1"
                     /translation="MENHLHIITGHYGSGKTEFAVNFAVSLARKGYDVTLADLDIVNP
                     YFCSREQTQALNGRGVRVIASLGGGADLPALNPAVASLFEPGVCGVIDMGGDPAGARV
                     LGRYAEAIRAVPHELLCVLNFNRPETATPEQAERYLRGIEHSAKLAATGLIHNTHLCS
                     ETSAADVLRGAKLAEEVGKRTGLPVLYHAFPRWIKGLTGLAPETLFPMDIMMKKPWET
                     DCEPEPEPD"
     gene            complement(917..1984)
                     /gene="buk"
                     /locus_tag="PLR57_03740"
     CDS             complement(917..1984)
                     /gene="buk"
                     /locus_tag="PLR57_03740"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HQQ40600.1"
                     /translation="MPYRILAINPGSTTTKIAVYDDETPLFETTINHTREEFSSCADL
                     VASLPIRKGAIEAELQSHGISARSLNAVIGRGGLLRPIAGGTYTINESMLSDLRSCKY
                     GEHACNLGAMLADEIARSVGVPAYIADSVVTDELCPLARLSGHPEISRVSIFHALNQK
                     AEARRFCTSRGLKYEEVNLIVAHIGGGATIGAHEKGRVIDVNDGLGGEGPYSAQRCGG
                     LPANSLIKLCAKENLPATDMIRKLNTKGGLLAYLGTDDGKEISRRAQSGDETARLVYE
                     GMAYQTAKAIGACAAVLKGDIRAIILTGGFAYDPLFTGWIAERTSFLAEIVVMPGEDE
                     QKALTEAALRVLRGEEQLKVY"
     gene            complement(1986..>2600)
                     /locus_tag="PLR57_03745"
     CDS             complement(1986..>2600)
                     /locus_tag="PLR57_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HQQ40601.1"
                     /translation="VSTASMLREVVNAETGLRESELLSYVSVFELPGFDRLIYLTDPA
                     MNMYPDVTAKKHIIENAVKVAGVLGAKKPVVGCLCAVETVNPKMQCTLDAAELVRKNR
                     EGELPDCIVTGPLALDNCLFREAAIHKGIEDPNAGKTDILLAPEIETGNALYKSFAFV
                     MKATCAGVIVGASAPVILTSRADREETKLNSIALAMRIAYERMN"
BASE COUNT          442 a          780 c          794 g          584 t
ORIGIN      
        1 cattcttgcg cagctcgcgc agccggtgca ttcgctcatg cgctccacga ttgcgggatg
       61 ataacccttt gcgtttgtcg cgctgtcgat gacgatgatg tgtttgggac acaccagcac
      121 gcaaagttca cagcccttgc agcggtttcg gtcgaaggtt acggttcctg ccatggcgtt
      181 gcctccttaa tcgggttcgg gttccggctc gcagtcggtt tcccagggtt tcttcatcat
      241 gatgtccatt ggaaagagcg tttccggcgc gagaccggtc agccccttga tccagcgcgg
      301 aaacgcgtgg tataaaacgg ggagccctgt gcgcttaccg acctcttcgg ccagtttcgc
      361 accgcgcaga acatccgccg ccgaggtctc ggagcagagg tgcgtattgt ggatcagccc
      421 cgtagccgcg agctttgcgc tgtgctcaat cccgcgcagg taccgttccg cctgttccgg
      481 cgtggcggtt tccgggcggt tgaagttcag tacgcagagg agctcatgcg gaacggcgcg
      541 gattgcttcg gcgtaccgcc caagcacgcg cgcgcccgcc gggtcgccgc ccatgtcgat
      601 taccccgcat acgccgggtt cgaacagcga tgcaaccgcc ggattcagcg cgggcagatc
      661 cgcgcctccg cccagagagg cgattacgcg cacgccgcga ccattcagcg cctgcgtctg
      721 ctcccgcgag cagaagtagg ggttgacgat atccaggtcg gcgagcgtca catcatagcc
      781 ttttctggcg agagatacgg caaaattgac cgcgaattcc gtttttccgc tgccgtagtg
      841 gcctgttatg atgtgcaggt ggttttccat atcgttcgct ctttccgccg gaacaggatt
      901 gttcaaatgc gagtggtcaa tatactttta gctgttcctc gccgcgcaga acgcggagcg
      961 ccgcttcggt gagagctttc tgttcgtcct ctccgggcat gacgacgatt tccgccaaga
     1021 acgacgtccg ttcggcaatc cagcccgtaa acagcggatc gtaggcaaac ccgccggtga
     1081 gaatgatggc gcggatgtcg cctttcagca cggcggcgca ggctccgatc gcttttgcgg
     1141 tctggtaagc cattccttcg taaacgaggc gcgctgtttc gtccccactc tgcgcgcggc
     1201 ggctgatttc cttgccgtcg tccgtgccga ggtaggcgag aaggccgcct tttgtgttga
     1261 gcttgcggat catatcggtt gcgggcaggt tttcttttgc gcagagcttg ataagcgagt
     1321 ttgccggcag gccgccgcag cgctgcgcgc tgtaaggccc ttcgccgccg agcccgtcgt
     1381 ttacgtcgat gacgcgccct ttttcatgcg cgccgatggt cgcgccgccg ccgatatgcg
     1441 caacgatcag attgacttcc tcatatttca gcccgcggct cgtgcagaaa cgccgcgctt
     1501 ccgccttttg gttcagtgcg tgaaaaatag aaacgcggct gatctccgga tggccggaga
     1561 gccgcgctaa ggggcagagt tcgtccgtca cgacggagtc cgcaatatag gcgggaacgc
     1621 ccacggaacg ggctatttcg tcggccagca ttgcaccgag gttgcaggcg tgctcgccgt
     1681 atttgcagct cctgaggtcg ctgagcatgc tctcgttaat cgtatacgtt ccacccgcga
     1741 tggggcgtaa aagcccgccg cgtccgatga cagcgttcag ggagcgggcg gatatcccgt
     1801 ggctttggag ttccgcttcg atggcgcctt tccggatcgg cagcgaagcc accagatccg
     1861 cgcaggagga gaattcctcg cgcgtgtggt tgatcgtggt ttcgaatagt ggcgtttcgt
     1921 cgtcgtaaac cgcaattttc gtggtggtgg agccggggtt gatcgcgaga atccggtacg
     1981 gcatgctagt tcatcctttc ataagcgatg cgcatggcaa gcgctatgga gttgagcttt
     2041 gtctcttcgc ggtcggcgcg gcttgtgaga atcaccggcg cggaagcgcc aacgatcacg
     2101 cccgcgcagg ttgccttcat cacgaacgca aacgacttat acagcgcgtt gcctgtttcg
     2161 atttccggcg cgagcaggat gtccgttttc cccgcgttgg ggtcttcaat tcccttatgg
     2221 attgcggcct cccgaaacag gcagttgtcc agcgccagag ggccggtgac gatgcagtcc
     2281 ggcagctcgc cttcgcggtt tttacgcacc agttccgccg cgtcgagcgt gcactgcatc
     2341 ttggggttga cggtttccac cgcgcagaga cacccgacga cgggcttttt tgcgccgaga
     2401 acgcccgcaa ccttgaccgc gttttcaatg atgtgcttct ttgcggtcac gtcggggtac
     2461 atgttcatcg ccgggtcggt aagatagatc agccggtcga atcccggcag ctcaaacacg
     2521 ctcacatacg agagcagctc gctttccctg agccccgttt cggcgttgac cacctcgcgc
     2581 agcatggaag ccgtgctcac
//
LOCUS       DAOMGY010000188         2599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_43498_length_2599_cov_2.712264, whole genome shotgun sequence.
ACCESSION   DAOMGY010000188 DAOMGY010000000
VERSION     DAOMGY010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43498_length_2599_cov_2.712264"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..405)
                     /locus_tag="PLR57_03750"
     tRNA            complement(323..405)
                     /locus_tag="PLR57_03750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Leu,seq:taa)
     gene            complement(491..1060)
                     /locus_tag="PLR57_03755"
     CDS             complement(491..1060)
                     /locus_tag="PLR57_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HQQ40602.1"
                     /translation="MTYLLLFLEFLQTGLFSVGGGYATLPFLQKIMERYPDWFGSLQL
                     ADIAAIAETTPGPVGVNAATFAGYSAAGVPGALISTFALVLPSFIIVSLIARALEKYR
                     GNKLVGDLFSGLRPAVTGLIAAAAWSVIHAALMSGAIADGIWQAIDWKNVVLFFVFFG
                     LMMLKKLKNVHPVFFFLAGGAAGALMGLL"
     gene            complement(1057..1587)
                     /locus_tag="PLR57_03760"
     CDS             complement(1057..1587)
                     /locus_tag="PLR57_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HQQ40603.1"
                     /translation="MKNLWELFISFLKIGALMFGSGYTMLPLLTHEVVERRGWVTEEE
                     MLDYFALAQCAPGVIAVNTATFIGQKRGGWAGAAAAIAGVICAPILAILLVAIVLLQF
                     WDNPIVVNVFNGVRVAVAALITTAVIRLFRANVKDWFGIALCIVGFLIAVFGLSPIFV
                     VAVGVVAGLVAGRVRK"
     gene            complement(1641..>2599)
                     /locus_tag="PLR57_03765"
     CDS             complement(1641..>2599)
                     /locus_tag="PLR57_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40604.1"
                     /translation="VNLGDDRLLIVFLPLYPILVKIFHYVFGSYLASGLFVSNVCWLL
                     AAYMLYELALLDTNRRGALRALKYLCILPASFLFASPLSDSLFLLLSLLCVYCVRKGL
                     YPFAGIAGFFASFTRMPGILLLAPAIFELVGQIIRERRAHATDVRWKRRMVGSALSLL
                     LIPCGLLVYLYVNYSVTGNALMFLTYQKEHWHQQLGWFFSSTATIATSATTTFFSNAP
                     MVWGLWIPNLVYLLASLGIVLAAQNKLRASNVAYFIAYYAVCMGATWLLSAPRYLTAA
                     YPLALALGALTEKKWADRLATGVCIVLFILYLIAFVNQWYVY"
BASE COUNT          674 a          721 c          734 g          470 t
ORIGIN      
        1 ggagagccac gaagtactcg gcaaaaaaga aagaatagcc caactaacac ctctgattaa
       61 agtgtttggg cagagcattt tgaacacttt tatttttcct tgtactccgt tatttttcgt
      121 agaactattt tatttttcct cttagggtat tcaaattgat atcctgtgcc gggagttccg
      181 ataaaccccg ggaacgcaaa aaagcctgca atacaaagga ttttgaatac ccctacgggg
      241 caaacccctt gtattgcagg ctttctgaga cgaataataa aaggattgac cgaagcatcg
      301 tatcaatgct tcggtcaatg tttggtgcga ccggagggat ttgaaccctc aaggtttccc
      361 cgacggattt taagtccgtt gtgtatgccg ttccaccacg gtcgcaacgg gaatatttta
      421 ccatgctggg agcgaaagtc aattcgcttt ctcgaagttt tgtcggcgtt tgaattttac
      481 ggaaccgctc ctaaagcaaa cccatcagcg ctcccgccgc gccgcctgcg agaaaaaaga
      541 acaccggatg gacgtttttc agctttttga gcatcatcag cccgaagaac acgaaaaaca
      601 gcacgacgtt tttccagtcg atcgcctgcc aaatgccgtc cgcgattgca ccgctcatca
      661 gcgccgcgtg gatgaccgac caggccgcgg cggcaatcag ccccgtcacg gcgggacgca
      721 ggccggagaa cagatcaccc acaagcttgt ttccgcggta cttctcgagc gcgcgcgcaa
      781 tcagcgaaac gataataaac gaaggcagca ccagcgcgaa ggtggagatc aacgcgccgg
      841 gaacgcccgc cgcgctgtag cccgcaaacg ttgcggcgtt gacgccgacg gggccgggcg
      901 tcgtttccgc aatggcggcg atatcggcga gctggagcga gccaaaccag tccgggtaac
      961 gctccataat tttctgtaag aacggcaggg tggcataacc gccgccaacg gaaaacaaac
     1021 ccgtttgcag gaactcaaga aacagcaaaa gatacgtcat ttccgcaccc tccccgcaac
     1081 aagtcccgca acgacgccga cagcgacgac gaaaatgggc gacagcccga acacggcgat
     1141 gagaaacccg acgatgcaca gcgcaatgcc gaaccagtct ttcacgttgg cgcggaacag
     1201 ccggataacc gccgtcgtaa tcagcgccgc gaccgcaacg cgcacgccgt tgaacacgtt
     1261 aaccacgatc ggattgtccc aaaactgcag cagcacgatg gcgaccaaaa ggatggccag
     1321 aatgggagcg caaattacgc ccgcaatcgc cgcggcagcg ccagcccaac cgccgcgttt
     1381 ctggccgatg aacgttgcgg tgttgacggc gatgacgcct ggggcgcatt gcgcgagagc
     1441 gaagtagtcc agcatttctt cttccgtgac ccagccgcgg cgttcgacga cttcatgtgt
     1501 caaaagcggc agcatggtat acccgctgcc gaacatcagc gcgccgattt ttaaaaacga
     1561 tataaagagt tcccagagat ttttcatacg gtttcgatcc tgcgttggat tcggctgacc
     1621 cgccgtgcgg ttctgcccgt tcaatagacg taccattgat tgacaaacgc aatcaggtag
     1681 agaatgaaca gcacaataca gacgccggta gccagccggt ccgcccattt cttttcggtg
     1741 agcgcgccga gcgccagcgc gagcgggtac gcggcggtta aatacctagg tgcggagagc
     1801 agccaggttg cgcccataca gacggcgtag taagcgatga agtaggcaac gttcgaagcg
     1861 cggagcttat tctgggctgc gagtacgatg ccaagcgaag cgagaagata cacgaggttc
     1921 ggaatccaga gcccccaaac cataggcgcg ttgctgaaaa aggtcgtcgt cgcgctcgtg
     1981 gcaatggtcg ccgtcgagga gaagaaccag ccgagttgtt gatgccaatg ctccttttgg
     2041 taggtgagga acatcagcgc gttgccggtg acggagtagt tgacgtagag atataccagc
     2101 agcccgcagg ggatgagcag aagcgagagc gcgctgccaa ccatgcgccg cttccagcgg
     2161 acgtcagtgg cgtgcgcgcg gcgttcgcgg ataatctggc cgacaagctc gaagatggcg
     2221 ggcgccagca gcagaatgcc aggcatccgc gtaaacgagg cgaaaaagcc cgcaatgccc
     2281 gcgaacggat agagcccttt tcgcacgcag tatacgcaga gcagtgaaag caataaaaac
     2341 aggctgtccg acagcgggga agcaaagagg aacgacgcgg gcagaatgca gaggtacttc
     2401 agcgcccgca gcgcaccgcg gcggttggta tccagcagcg ccagctcata aagcatgtaa
     2461 gccgcgagca gccagcagac gttggaaaca aacagtccgc tggcaagata ggagccgaat
     2521 acatagtgga atatcttcac cagaatcgga tacagcggta aaaacacgat cagcagccgg
     2581 tcgtcgccaa ggttcacat
//
LOCUS       DAOMGY010000189         2593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_43645_length_2593_cov_5.126872, whole genome shotgun sequence.
ACCESSION   DAOMGY010000189 DAOMGY010000000
VERSION     DAOMGY010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43645_length_2593_cov_5.126872"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLR57_03770"
     CDS             <1..560
                     /locus_tag="PLR57_03770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ40605.1"
                     /translation="NDLSTLPESSPARLRKLEIFDINELIRRTLITFEARIYEHHMEV
                     DVKFAQEQYYVEADSAQISQVLRNIIDNAIKYSPDDSKLRIATYAMKREIYVSIQDSG
                     QGIPEQDIPHVFDRFYKVEKAHTPSKQSGTGLGLSIVKRIIDQHNQTITLKSAKGKGS
                     TFTFTLKRAPAPKRAPTDGGKKNGI"
     gene            550..1905
                     /locus_tag="PLR57_03775"
     CDS             550..1905
                     /locus_tag="PLR57_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQQ40606.1"
                     /translation="MEYNGSYQSDKRHPENGRGENAFAAIVIIIVLILLALLIVGLFW
                     PSLLGGSSADLAVITPTPSVSAAPTPEETAGQDAQLPASPTLTPAVDENDRVMPELDG
                     TKPNVPGLVENPIPDIYDAVSPGVVGVINYTKATSPSGKEYFEIYGSGTGFAVSSEGY
                     ILTNAHVVEGADKVTVKVDGEDEERDAVIIGSDNETDIAVLKVEGATLHPLMLGDSDS
                     VRVGEYVLAIGNPLSTDELANTLTFGIISATSREITIDSYTNTYLQTDAAINFGNSGG
                     PLINMDGLVIGINSAKTITAGYDELGNAVSAEGIGFALPINQVKKIMEILITEGKIER
                     PGIGITVTTITEDIAAENDVPVGAYVESVVIGQPSDIAGLLAGDIIVEANGKTIKTQD
                     DLVSVIKSCLVGDELKIKVYRDGEYLDLTIIIGNKTDMNFDDIVGGVTPTPVPSLIPV
                     P"
     gene            complement(2055..>2593)
                     /locus_tag="PLR57_03780"
     CDS             complement(2055..>2593)
                     /locus_tag="PLR57_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40607.1"
                     /translation="IGGGSTQLVKASSRESWPIGCVRAKDLAPFDELRQIMDALRPVL
                     SSLFGDPAQDDRPDAQSIEAIRRNQSAVPLGVGGTITTLALLCEGKPEFTTWEGEKPV
                     EFLDLCATLQTLSNMTDGERAELPLLKDRHDVILHGGVILLYLMERLHTTRVGATLSD
                     GLDGYGAYLFETLKKKKL"
BASE COUNT          625 a          743 c          722 g          503 t
ORIGIN      
        1 tcaacgacct ctcgaccttg ccagaatcga gtccggcaag actgcgcaag ctggagatat
       61 tcgatatcaa cgagttgatc cgcagaaccc tgattacctt cgaagcgcgc atctatgagc
      121 accatatgga ggtggacgtt aagttcgcac aggagcagta ctacgtggaa gccgacagcg
      181 cgcagatttc gcaggtgctt cgcaacatca tcgacaacgc gatcaagtat tcgccggacg
      241 acagcaagct cagaatcgcg acctatgcca tgaagcgcga aatctacgtc agcattcagg
      301 acagcggaca gggcattccc gaacaggata ttccgcacgt gttcgaccgg ttctacaaag
      361 tggaaaaggc gcatacgcct tccaaacaga gcggcaccgg attgggtctt tcgattgtca
      421 agcgcatcat agaccagcat aaccagacaa taaccctgaa gagcgcaaaa ggaaaaggaa
      481 gcacgttcac attcacgtta aagcgcgcac ccgcacccaa acgcgcgccg acggatggag
      541 gaaagaaaaa tggaatataa cggttcttat caatcggata aacgtcaccc ggaaaacggg
      601 cgaggcgaaa acgcgtttgc cgcgatcgtc atcatcatcg tacttatttt gctggcactg
      661 ctgattgtcg gcctgttctg gccgtcgctt ctcggcggtt cctcggcgga cttggctgtg
      721 attacgccaa cgccctccgt cagcgcggct cctacgccgg aagaaacggc ggggcaggat
      781 gcgcagctgc ctgcaagccc cacgcttaca ccagccgtgg atgaaaacga ccgcgtcatg
      841 ccagaactcg acggcacaaa gccgaacgtc cccggactag tggaaaaccc gattccggat
      901 atttacgacg cggtttctcc tggcgttgta ggcgttatca actacacaaa ggcgacaagc
      961 cccagcggca aggaatattt cgaaatctac ggcagcggta cgggctttgc cgtgtccagc
     1021 gaagggtata tccttaccaa cgcgcatgtc gtggagggcg cggacaaggt taccgtcaag
     1081 gttgacggcg aggacgaaga gcgcgacgct gtcattattg gttccgataa cgaaacggat
     1141 atcgccgtgc tcaaggtgga gggagcgacg ctccatccgc tcatgctggg cgattcggac
     1201 tccgtgcgcg taggcgaata cgtgctcgcc atcggcaatc cgctttccac ggacgaactg
     1261 gccaacacgc tgaccttcgg cattatcagc gccacttcgc gcgagattac gatagacagc
     1321 tatacgaaca cgtaccttca gaccgacgcc gccatcaact ttggcaacag cggcggcccg
     1381 ctgatcaaca tggacggcct cgtaattggc atcaacagcg caaagacgat caccgcgggc
     1441 tatgacgagc tcggcaacgc cgtcagcgcg gaaggcatcg ggtttgcgct gccgatcaac
     1501 caggtgaaga agatcatgga gattctgatc accgaaggca agatcgagcg tcccggcatc
     1561 ggcattacgg tcacaacgat tacagaggat atcgccgcgg aaaacgacgt cccggtcggc
     1621 gcgtatgtcg agagcgtcgt aatcggccag ccttccgata tcgcgggctt gcttgccggt
     1681 gatatcattg tggaagcgaa cggcaagacc atcaaaacgc aggacgatct ggtcagtgtc
     1741 atcaagagct gcctcgttgg cgacgaactc aagatcaagg tctaccgcga cggagagtat
     1801 cttgacctta cgatcatcat cggcaacaag accgatatga acttcgacga tatcgtcggc
     1861 ggggtaacgc ctaccccggt tccgtcgctg attccggtgc cgtaaccaat caaaattaaa
     1921 agaagcgctc cgtaacagga gcgtttctct ttatgccgga caaacctgtg ggttctcagg
     1981 gcgacagctc taggcgggtg caggagggcg gagccctcaa atcgatctcc tctaaattaa
     2041 cgggtttcct accgttaaag cttttttttc tttagcgttt caaaaaggta tgcaccgtag
     2101 ccatccagcc cgtcggaaag cgttgcgcca acgcgcgttg tgtgcaaacg ttccatgagg
     2161 taaagtagaa tcacgccgcc atgcagaatc acgtcgtgcc gatccttgag cagcggaagt
     2221 tccgcccgct cgccgtcggt catgttgctc agcgtttgca gcgtggcaca caggtcgaga
     2281 aactcaaccg gcttttcgcc ttcccacgtc gtaaactccg gcttgccttc acagagcagc
     2341 gccagagtcg taatcgttcc gccaacgccg agcgggacgg cgctttggtt cctgcggatg
     2401 gcctcgatcg actgcgcatc tgggcggtcg tcctgggctg gatcgccgaa caaactcgaa
     2461 aggacggggc ggagcgcatc cataatctgc ctcagttcat cgaacggcgc aagatccttt
     2521 gcgcgtacgc acccgatggg ccagctctcg cgcgaggagg cttttacgag ctgcgtactg
     2581 ccgccgccga tat
//
LOCUS       DAOMGY010000190         2591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_43693_length_2591_cov_3.232256, whole genome shotgun sequence.
ACCESSION   DAOMGY010000190 DAOMGY010000000
VERSION     DAOMGY010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43693_length_2591_cov_3.232256"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PLR57_03785"
     CDS             complement(<1..1108)
                     /locus_tag="PLR57_03785"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQQ40608.1"
                     /translation="MEKEAVPQTAPEIPAAGRREKNTRFDEKHIDVLDGIRALAVLGV
                     LWFHFWQQNWISPYFKLPFLTSLGLSGSVSLDFLPRAGFLFVDLLLFLSAFCLFLPYV
                     RASLDGAALPKAGQFYKKRIARIVPSYYFAVFVILFTVALPSGEYGSAEECLRDLLPT
                     LTFTQTLFPNILLGTKLNGVLWTAAIEMQFYLFFPLLAAAFVKKPGWTYLGMAAVAAL
                     YLRGFALNNPDSIRSTLNQLPGFFGVFANGMAVAYLYVLFARRVKRSAQLSAVALCAL
                     IAGIWILVGMMKAAPGTNPVHLWQAENRFKLSLVFALIVVSSALTFSGVRWFFSNAVM
                     RFLSAISYNLYIWHQWISVKFKQWKIPYWTGDTPP"
     gene            complement(1349..>2591)
                     /locus_tag="PLR57_03790"
     CDS             complement(1349..>2591)
                     /locus_tag="PLR57_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40609.1"
                     /translation="LAQVKGLKFEYISIGVTHTHNSSDFYSDFMREDNERVYGADILP
                     KIELAEDVLTGEEAQEFLVSRLADVVTRAWESRKSGAVSMAHDYAAVGFCRRPVFRGK
                     TDETVMYGDCSREDFIRFEPGGDSSADMLYTWDEQGELTGILVNIPCPSQVFELHRFI
                     TADYWGFARDEIRERLGNVYILPLCGAAGDLSPLDLVRISKYNKKELVEWGGQAKEVF
                     RNFDMTRVCQEIAERIADAVARGYQRARNEMEQTPAFGHEVLELKLPLRLVTREEYEI
                     AAREVARFKSTFSSEHRMTEEDVVKAFEPQGDVLRWEQQQVSCEFDCACHVLRIGNAG
                     IATNPFELFTEYALRMKARVIPEQLFIGQLSNGVGGYLPTESALAGGSYSSKSASSFC
                     GPEGGDALVEKTIEAVNRLFS"
BASE COUNT          604 a          799 c          695 g          493 t
ORIGIN      
        1 tcggcggggt gtcgccagtc cagtacggaa tcttccactg cttgaacttg acgctgatcc
       61 actggtgcca gatatagagg ttatacgaaa tcgcgctcaa aaaccgcatc accgcgttgg
      121 agaaaaacca gcgcacgccg gagaacgtca gcgcgctgga aacgacgatc agcgcgaaca
      181 ccagcgagag cttaaaacgg ttttccgcct gccagagatg cacggggttc gttccgggtg
      241 cggctttcat catgcccacg agaatccata ttcccgcgat gagcgcgcag agcgccaccg
      301 ccgaaagctg cgcgctgcgt ttaacgcgtc ttgcaaacag cacgtaaagg tacgcaaccg
      361 ccatgccgtt ggcaaacacg ccaaagaacc ccggcagctg gttgagcgtc gagcggatgg
      421 agtccgggtt gttcagcgca aatccgcgca ggtacagcgc ggcaacagcc gccatgccga
      481 gatacgtcca gcccggcttt ttaacaaacg ccgcagccag cagcggaaag aacaggtaaa
      541 actgcatctc gatggccgcc gtccagagca cgccgttgag cttggtgccc agcagaatat
      601 tcggaaacag cgtctgcgta aatgttagcg tcggcagcaa atccctcagg cactcctccg
      661 cggagccgta ctcgccggaa ggaagcgcca ccgtaaacag aatcacgaac accgcaaaat
      721 agtagctcgg cacgatgcgg gcgatgcgct ttttatagaa ctgtcccgct ttcggcagcg
      781 ccgcgccgtc gagggaagcg cggacatacg gcagaaacag gcagaacgcc gagaggaaca
      841 gcaagagatc gacaaacaaa aaccccgcgc gcggcaagaa atcaaggctg accgaccctg
      901 aaagcccgag cgaggttaaa aacggaagtt tgaagtaagg tgagatccag ttctgctgcc
      961 agaaatgaaa ccaaagcacg ccgagcaccg ccagcgcgcg gataccgtca agcacgtcga
     1021 tatgcttctc gtcgaagcgc gtattctttt ctcgtctgcc cgccgccgga atctccggag
     1081 ccgtctgcgg gaccgcctct ttttccatgg cgatatgata ccacaaaccc cttgttttgg
     1141 gaaagccgga acaagctgtt ttttctttcc cagttaatcg ccctgccgta aaaaaatggc
     1201 aaagccgcct tgcaaacgca aagcggcctt gtccggagaa aaaaccatga aaaagaccaa
     1261 tgtttttcac gagaattccg ttttcgcctt gagcagagct ttttcaagca tccgcacgcg
     1321 aagattctcc cgaaagaatc gtcgccgctt acgaaaacag gcggttcacc gcttcaatgg
     1381 tcttctccac cagcgcgtca ccgccttcag gcccgcaaaa gctggaagcg gatttgctgc
     1441 tgtagctgcc gcccgcaagg gcggactccg tcggcagata gccgccaacg ccattggaaa
     1501 gctggccgat aaagagttgc tcagggatta cgcgcgcttt catgcgcagc gcgtactccg
     1561 taaacagctc gaacgggttg gtcgcaatac ccgcgtttcc gatgcgcaat acgtggcagg
     1621 cgcagtcaaa ctcacaggaa acctgctgct gctcccagcg gagcacatcg ccctgcggtt
     1681 caaacgcctt gacaacgtcc tcttccgtca tgcggtgttc gctggaaaac gtgctcttga
     1741 atcgcgcaac ctcgcgcgcc gcgatttcgt actcctcgcg cgtgaccaac cggagcggga
     1801 gcttgagctc cagcacctcg tgcccaaacg cgggggtctg ttccatttcg ttgcgcgcgc
     1861 gctgataacc ccgcgccacc gcatccgcga tgcgctcggc aatctcctgg caaacgcgcg
     1921 tcatgtcgaa attgcggaac acttccttcg cctgaccgcc ccactcgact agctctttct
     1981 tattatactt gctgattcga actaaatcca gtggtgaaag gtctccggca gcgccgcaaa
     2041 gcggtaaaat atatacattt ccgagccgtt cgcgaatttc gtcccgcgca aagccccaat
     2101 agtccgccgt aatgaaccgg tgcagttcaa aaacctgcga gggacacgga atgtttacca
     2161 gtatgcccgt cagctcgccc tgctcgtccc aggtatagag catgtccgcc gaactgtccc
     2221 cgcccggctc aaagcggata aaatcctcgc gcgagcagtc gccgtacatc acggtttcgt
     2281 ccgttttgcc gcgaaagacc ggcctgcggc aaaagccgac cgctgcgtag tcgtgcgcca
     2341 tgctcacggc gccggatttc cggctttccc aggcgcgggt cactacgtcc gcaaggcgcg
     2401 aaaccagaaa ttcctgcgct tcctcgccgg tgagcacgtc ttccgccagt tcgatcttcg
     2461 gcagaatgtc cgcgccgtac acgcgttcat tgtcctcgcg catgaaatcc gagtagaaat
     2521 cggaagagtt gtgcgtgtgc gttaccccga tggagatata ttcgaacttg agccctttta
     2581 cctgcgcgag c
//
LOCUS       DAOMGY010000191         2585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_43838_length_2585_cov_3.271146, whole genome shotgun sequence.
ACCESSION   DAOMGY010000191 DAOMGY010000000
VERSION     DAOMGY010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43838_length_2585_cov_3.271146"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2321)
                     /locus_tag="PLR57_03795"
     CDS             complement(<1..2321)
                     /locus_tag="PLR57_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HQQ40610.1"
                     /translation="MLKYELQYPALGAHLNSIRRRVYRVVCRLNAEILPSAEPIPFSE
                     LGQHSFTEASRGASWGRKLSCAWLRISGSVPEGLNNPVLLVHNTGEGLLYTPEGEILD
                     GLSDVWAPNDIPRASGKCVSLRLPGLTAGKAFTFYMDYGYNGIVLNDIGRARFFGAFL
                     PETDEGVYAYYYDYITLFVLLAETGDAEKERELSRALERSYRVFVKEGANAAREVLAV
                     PLSKRSRDPFTFQAVGHGHLDLAWMWPIRETMRKSARTYAMALSNIARYPDYIYGTSQ
                     PQQLQWMKERNPLLYARIRQAIIDGRIELQGGFWTECDCNLSGGESLVRQAVYGTRFA
                     EREFGKQMRICWLPDAFGFNGNLPQILRGCGMEYFSTIKLAWNKVNVFPYRSFLWRGV
                     DGSEVLVHMPPEGDYNSGAAANGIFKALRQYPERALETALLVYGGGDGGGGPRESHLE
                     LLARERDLEGLPKIAFSSAIAFFDSLKQKTIPHTYTGELYLETHQGTYTTQSENKRYN
                     RLMERLLHNLEALCSLLSKKEYPYEALEEIWKEVLLYQFHDILPGSSIHRVYTESCAG
                     YAKMEQRLHALTEQAIPKADTVTAVNLTSFPRSEYVRWQDGWRHAEAAPYSAVEAVPF
                     SGSAEALSYTERTMSNGLLTLTFNEYGEIASCKIADGRELAGGTLNRLTLYRDKFTIP
                     FDAWDIDAKYYKKHCRRLRASIIETKIDGPRVVRKQVYCFGKSSVTQSVILELGGERV
                     LFDTECSWHERFKMLRADFYPADYGDTAEFDIQ"
BASE COUNT          543 a          748 c          755 g          539 t
ORIGIN      
        1 aactggatat cgaattcggc ggtatcgccg tagtctgcgg ggtagaagtc cgcgcggagc
       61 attttgaagc gctcgtgcca gctgcactcc gtatcaaaca acacgcgctc cccgccaagt
      121 tcgaggatga cactttgcgt aacgctggat ttgccgaaac agtagacctg ttttcgcacc
      181 acacgcgggc cgtcgatctt ggtttctatg atggaagcac gaagcctgcg gcaatgtttc
      241 ttataatatt ttgcgtcgat atcccacgcg tcgaagggga tggtgaactt gtcgcggtag
      301 agcgtcaggc ggttgagcgt gccgccggcg agttcgcgcc catcggcgat tttacaggag
      361 gcgatctcgc cgtattcgtt gaacgtaagc gtcaaaagcc cgttgctcat cgtgcgttcg
      421 gtgtacgaaa gcgcttccgc cgacccgcta aacggtaccg cctcgaccgc ggaatacggc
      481 gcggcctcgg catgccgcca gccatcctgc cagcggacat attccgagcg cgggaacgaa
      541 gtcaggttga ccgctgtgac ggtgtccgct ttgggaatcg cctgctcggt cagcgcgtgc
      601 agccgctgct ccattttggc atatcccgcg caggattccg tgtagacgcg gtggatgctc
      661 gaaccgggca aaatatcgtg gaactggtag agcagaactt ccttccagat ctcctccagc
      721 gcttcatacg gatattcctt ctttgacagc agtgagcaaa gcgcttccag attgtgcagc
      781 aaacgctcca tcaggcggtt atagcgcttg ttttcggact gcgtggtata ggtgccctga
      841 tgcgtttcta aatagagttc gccggtatag gtatgcggaa tcgttttctg cttgagggag
      901 tcgaaaaacg caatcgcgct cgaaaacgca atctttggca ggccttccag gtcgcgttcg
      961 cgggcgagca gttccaaatg gctctcgcgc gggccgccgc cgccgtcgcc gccgccgtag
     1021 accaaaagcg cggtctccag cgcgcgttcc ggatattgcc ggagtgcttt gaagattccg
     1081 ttcgcggcgg ctccgctgtt gtagtcccct tccggcggca tatggacgag cacttcgctg
     1141 ccgtccacgc cgcgccagag gaacgagcgg tacggaaaaa cgttgacctt gttccacgcg
     1201 agtttgatgg tggaaaaata ttccatgcca cagccgcgca gaatctgcgg caggttgccg
     1261 ttaaagccga acgcgtccgg cagccagcaa atgcgcatct gtttgccgaa ctcccgctcg
     1321 gcaaagcgcg tgccgtagac agcctgccga acgagcgatt caccaccgga taggttacag
     1381 tcgcactccg tccagaagcc gccctgcagc tcgattctgc cgtcgatgat cgcctgccgg
     1441 atgcgggcgt agagcaaagg gttacgctcc ttcatccact gcagctgctg gggctggctg
     1501 gtaccgtaaa tatagtccgg gtagcgggcg atgtttgaaa gcgccatggc gtaggtgcgc
     1561 gcgctctttc gcatggtttc gcggatgggc cacatccagg caaggtcgag gtgcccgtgt
     1621 cccacggcct gaaacgtaaa cgggtcgcgg gaacgcttgg agagcggaac cgcaagcact
     1681 tcgcgcgcgg cattcgcgcc ctctttgaca aacacgcggt aggaacgctc cagcgcgcgg
     1741 gaaagctcgc gttctttctc agcatctccg gtttcggcaa gcagtacgaa cagtgtgatg
     1801 taatcgtagt aatatgcgta tacgccctcg tccgtttcgg gaagaaacgc gccgaaaaac
     1861 cgcgcgcggc cgatatcgtt gagtacgatg ccgttgtagc catagtccat gtaaaaggta
     1921 aacgccttcc ccgcagtcaa cccgggcagg cggagggaaa cgcatttccc gctggcgcgc
     1981 gggatgtcgt tcggcgccca gacgtcgctg aggccgtcga gtatttcgcc ttccggcgta
     2041 tacaaaagcc cctcgcccgt attgtgcacg agcagcacgg gattgttcag tccctccggc
     2101 acgcttccgg aaatccgcag ccaggcgcag gaaagctttc gcccccacga agcgccgcgg
     2161 gacgcttccg taaacgaatg ctgacccagt tcgctgaacg gaatcggttc cgcgctgggc
     2221 agaatctccg cgttcaaacg gcatacgacg cggtaaacac gtctgcggat gctgtttaag
     2281 tgcgcgccga gcgccgggta ttgcagttca tatttgagca tagttcgcct cgaataacaa
     2341 aaataagaga taggaagaaa gagaacagat cgacggctcc cctgaacgga aaaaatacaa
     2401 gtcgagccat ctcttctggc atgctatcac atggcaaata ggctgtcaac gcactgccgc
     2461 cggcgatgtg cccgcaaatc cgtatgtcag attttcgccg cgctccgtac ggtgagaaca
     2521 agaaaaataa gtagaaatta atctgtcttt ccaccctttt tccggttcca ttccgcgcgc
     2581 ggacg
//
LOCUS       DAOMGY010000192         2576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_44040_length_2576_cov_3.140817, whole genome shotgun sequence.
ACCESSION   DAOMGY010000192 DAOMGY010000000
VERSION     DAOMGY010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44040_length_2576_cov_3.140817"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLR57_03800"
     CDS             complement(<1..339)
                     /locus_tag="PLR57_03800"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HQQ40611.1"
                     /translation="MEQHSEFNRIYEETYRDVLRYIVAKTNRADDVEDILQEVYRSFY
                     VRLCRSGEHGIRDAKRYLIVIAKKELTRFYRNRSTRAEKELPLTDDAAEAAEPLDESY
                     FTAEAAKRTWE"
     gene            575..2281
                     /gene="dnaX"
                     /locus_tag="PLR57_03805"
     CDS             575..2281
                     /gene="dnaX"
                     /locus_tag="PLR57_03805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQQ40612.1"
                     /translation="MAYRALYRQYRPAVFSEVIGQDHITTTLKNQVASDRPAHAYLFC
                     GTRGTGKTSTARILARAVNCLSPENGEPCGKCEACLLSAAEGASDITEIDAASNNSVS
                     DVRDLIESAQYAPLKLRRRVFIIDEVHMLSGAAFNALLKTLEEPPEHILFILATTEPQ
                     KLPATIISRCQRFDFHRLSVTDIKKTLKDVLSRAGASIADGGLSLIARAAQGGMRDAL
                     SLADQCLAFCGDNVSERDVYDVLGSMEDSFLFDISEALLSSNAQQALKMLDGIVHSGR
                     DLSVFCADLSAHFRALLLAKTCGRCEDLLDCTEDAMNRYLEQTKNTSEARLMLAVDRL
                     LSAQNEMRYLPSPRAMLESALIHICRPEDSVSLAALEARVDRLESGFVPAPAAQAKQS
                     AAPVSALNEPAPWDGPEPPPEGAYDSYAPAPEPKKAHAQAKKPEPKAEPAPQAKKTAS
                     DHAPAEPEIAPAADAPTDPVAIWRAAIAAIPNMFVQAIAREGVAIRFEHDTLTVGFSE
                     AQEVKYKNITAPINYEPVQRALQTVLPEAKLAFTLTRTAPASVRAEEMAKGLLGDKLE
                     IE"
     gene            2342..>2576
                     /locus_tag="PLR57_03810"
     CDS             2342..>2576
                     /locus_tag="PLR57_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQQ40613.1"
                     /translation="MAKGGFPGGLGGGANMQQLMMRAQRMQQDVQRTQAELAEREFSA
                     SAGGGMVTATVNGNKEVKAIVISKDCVDPDDVEM"
BASE COUNT          551 a          794 c          725 g          506 t
ORIGIN      
        1 ctcccatgtg cgtttcgccg cttccgccgt aaagtaactt tcgtcgagcg gttcggctgc
       61 ttccgcggca tcgtcggtca gcggaagctc cttttccgcg cgtgtgcttc tgtttcggta
      121 gaaacgcgtc agttcctttt tcgcaattac tatcaggtag cgctttgcgt cccgaatgcc
      181 gtgttccccg ctcctgcaaa gtcgaacgta gaagctgcgg tatacttcct gcagaatatc
      241 ttccacgtcg tcggcccgat tcgtttttgc aacgatataa cgcaaaacgt cccgataggt
      301 ctcttcgtag atgcggttga actcgctgtg ttgttccatc ctgttttcct ttcctatcga
      361 ctaaaaccgg tttcacctat aaagaggcgt ttttttaaaa aaagtaacac attggcgaaa
      421 tttcttcgat tttctatcga acagtccata atacagttcg atgggcttac ccccatagct
      481 cgccaacagg caaacccctt tggaatcccc gcttttaccg aatcgtgatt tatggtatca
      541 tactttttga atagagatca ggagaaacgc attcatggcc taccgcgcgc tctaccgcca
      601 ataccgcccc gccgtctttt cggaagtcat cgggcaggat catatcacca caacgctgaa
      661 aaaccaggtg gcgtcggatc gtccggcgca cgcctatttg ttctgcggca cgcgcggcac
      721 gggcaaaacc agcacggcgc gcatcctcgc ccgtgcggtc aactgccttt ctcccgaaaa
      781 cggcgaaccc tgcggcaagt gcgaagcctg cctgctgagc gccgcggaag gcgcttcgga
      841 cattacggaa atcgacgcgg ccagcaacaa cagcgtcagc gacgtgcgcg acctcatcga
      901 gagcgcgcag tacgccccgc tcaagctccg ccgccgcgtg tttatcatcg acgaagttca
      961 tatgctctcg ggagccgcgt tcaacgcgct gctcaaaacg ctggaagagc cgccggagca
     1021 cattctcttt atcctcgcga ccaccgaacc gcaaaagctc cccgcaacga tcatctcccg
     1081 ctgccagcgg ttcgactttc accgtttgag cgttacagac attaaaaaaa cgctcaagga
     1141 cgtcctctcc cgcgcgggcg cttccattgc ggacgggggg ctgtcgctca tcgcgcgcgc
     1201 ggcgcagggc ggcatgcgcg acgcgctttc gctggcggat cagtgcctcg cgttctgcgg
     1261 agacaacgtt tccgagcggg acgtttacga cgtgctgggc agcatggagg actcgttttt
     1321 attcgatatt tcggaggcgc tgctctcctc caacgctcag caggcgctca agatgctcga
     1381 cggcatcgtc cattccggac gcgacctttc ggtattctgc gccgaccttt cggcgcactt
     1441 tcgcgcgctg ctgctggcca aaacctgcgg caggtgcgag gatttactag actgcaccga
     1501 ggacgcgatg aaccgctatc tggagcagac gaagaatacg tccgaagcgc ggctcatgct
     1561 cgcggtggac cggctgcttt ccgcgcagaa tgaaatgcgc tacttaccct ctccgcgcgc
     1621 catgctggaa agcgcgctga tccatatctg ccggccggag gattccgttt cgctcgccgc
     1681 gctggaagcc cgcgtcgaca ggctggagag cggctttgtt ccggctcccg ccgcgcaggc
     1741 aaagcaaagt gcggctccgg tgagtgcgct gaacgaaccc gccccgtggg acgggcccga
     1801 accgccgccg gagggcgctt acgattccta tgcgcccgcg ccggaaccca aaaaagcgca
     1861 cgcacaagcg aaaaagccgg agccgaaagc ggaacccgcg ccgcaggcaa agaaaaccgc
     1921 aagtgatcac gcgcctgccg aaccggaaat cgcgcccgcc gcggatgcgc cgacagaccc
     1981 ggtcgccatc tggcgcgcgg ccatcgcggc gattccgaat atgtttgtgc aggccatcgc
     2041 gcgcgagggc gtcgccatcc gctttgagca cgacacgctg acggtcggat tttccgaagc
     2101 gcaggaggtc aagtacaaaa acatcaccgc gccgatcaac tacgaacccg tacagcgcgc
     2161 gctgcagacc gtactgccgg aagcaaagct cgcttttacg ctcacccgca ccgcgcccgc
     2221 gagcgttcgc gcggaagaaa tggcaaaagg gctgctcggc gacaagctgg aaattgagta
     2281 gatacacaaa acgattgata caagaacaga aagagaattt attttcagga ggatatgttt
     2341 tatggcaaaa ggcggattcc ccggaggact gggcggcggg gcaaacatgc agcagctcat
     2401 gatgcgcgcc cagcgcatgc agcaggacgt tcagcgcacg caggcggagc ttgcagagcg
     2461 tgaattctcc gccagtgcgg gcggcggcat ggtgaccgct accgtaaacg gcaacaagga
     2521 agtcaaagcg atcgtcattt cgaaagactg tgtcgatccg gacgacgtgg agatgc
//
LOCUS       DAOMGY010000193         2571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_44172_length_2571_cov_2.077901, whole genome shotgun sequence.
ACCESSION   DAOMGY010000193 DAOMGY010000000
VERSION     DAOMGY010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44172_length_2571_cov_2.077901"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLR57_03815"
     CDS             complement(<1..417)
                     /locus_tag="PLR57_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQQ40614.1"
                     /translation="MGKTPVKRIKSISYAKWGYFFIAPFFLVYLIFHLIPLGSTIYNS
                     FFENFRSGLTQIGPKFVGFQNYITLFQDGELPKFAWNTLVMWLMGFVPQILIAMLLAV
                     WFTSYRLKLKAQPFFKTVIYMPNLIMASAFAMLFFLL"
     gene            complement(551..1933)
                     /locus_tag="PLR57_03820"
     CDS             complement(551..1933)
                     /locus_tag="PLR57_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQQ40615.1"
                     /translation="MKKLLAILLTVAVLLSVCACSKPAETTAATTEEATAAAPAEAAA
                     PVEQGKVYNIYCWNDEFQARFEKYFVEAGLVPDGVTINWVITPNADNAYQNKLDEALL
                     AQETAAADDKIDLFLIEADYALKYVNTDYTLDVYTDVGLTEDDTAAMYGYTKDVCTDA
                     NGSLKGLSWQACPAGLIYRRSIAKAVLGTDDPAEVQAKLDSWDKFNTVAADAKALGYL
                     MVAGYDDDYRVFSNNATTTWVDENNKIQFDPNILAWIDQTKTFTDNGYNNKAILWSDE
                     QWAGAKADGTVMCYFGPGWFFDFCLAPATLADSEAEAAVGNGSYGDWAIVKGPQGYYW
                     GGTWLCAADGSDNLELTKLIMKTMTCDKDTLVGIVNDFNDFTNNEAAMTEIANSDFSS
                     AFLGGQNYISVLLDSAKSIKFLASPYDQGCNEKLQEAMRGYFNGTATYDEAMDNFYTL
                     VLEKYPELSR"
     gene            complement(2060..>2571)
                     /locus_tag="PLR57_03825"
     CDS             complement(2060..>2571)
                     /locus_tag="PLR57_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQQ40616.1"
                     /translation="IVRYVYSNGHRRIAFIHGEDTSVTQKRIASFFKACSEFDLRIPE
                     AYVRAAIYHDPRSSGLATRDLLALPERPTCILYPDDFSFIGGMNELERQGLSIPDDMS
                     VVGYDGILLSQVLRPKLTTFRQDAETMGRKAAALLVEQIENPKSWLPQSVTVSGTLLE
                     GHSVKTLNI"
BASE COUNT          539 a          679 c          742 g          611 t
ORIGIN      
        1 caggaggaag aacagcatgg caaacgcgga cgccatgatc aggtttggca tatagatgac
       61 cgttttgaaa aacggctgtg ctttcagctt gaggcggtaa ctggtaaacc agaccgcgag
      121 cagcatggca atgagaatct gcggcacaaa ccccatgagc cacatcacca gcgtattcca
      181 tgcaaatttc ggcagttccc cgtcctgaaa cagggtgata tagttttgaa acccaacgaa
      241 cttgggcccg atctgcgtga gccctgaacg gaagttttca aaaaagctgt tgtaaattgt
      301 tgaaccaagc ggaatcagat gaaatatcag atatacaaga aagaacgggg cgataaagaa
      361 atatccccat tttgcatagc tgatcgattt gattcgcttt acaggcgttt tacccataac
      421 ggcatccctt tcccttccag tttggtttgg ctttgccgga tgaacagcgg ctgctgcctt
      481 tctggaacga gacggcggaa cgggcatgcc cgtcccgccg tgcagcatct tatgttgtgt
      541 tcagagcaga ttatctgctg agttcggggt atttctccag cacgagcgtg tagaagttat
      601 ccatcgcttc atcataggtc gcggtgccgt tgaagtagcc gcgcattgct tcctggagct
      661 tctcgttgca gccctggtca tagggactgg cgaggaactt gatgctcttg gcgctgtcga
      721 gcagcacgga gatgtagttc tggccgccga ggaacgcgct ggaaaaatcg ctgttggcaa
      781 tttccgtcat cgcggcttcg ttgttggtga agtcgttgaa atcgttcacg atgccgacca
      841 gagtatcttt gtcgcaggtc atggtcttca tgatcagctt tgtgagctcg aggttgtcgg
      901 aaccatccgc tgcgcacagc caggtgccgc cccagtaata accctgcggt cccttgacga
      961 tcgcccagtc gccgtagctg ccgttaccaa cggcggcttc cgcttcggaa tcggccagcg
     1021 ttgcgggagc gaggcagaaa tcgaagaacc agccaggccc gaagtagcac atcacggttc
     1081 cgtcagcttt cgcgccggcc cactgttcgt cggaccagag gatcgctttg ttgttgtagc
     1141 cgttgtcggt gaaggtcttg gtctgatcga tccaggccag gatattcgga tcgaactgga
     1201 tcttgttgtt ctcgtcgacc caagtcgtgg tggcgttgtt gctgaatacg cggtagtcgt
     1261 cgtcataacc ggcaaccatg aggtagccca gcgctttggc gtccgcggcg acagtgttga
     1321 acttatccca actgtcgagc tttgcctgta cttctgccgg atcatccgtg ccgagaactg
     1381 ccttggcgat cgagcggcga tagatcagtc cggcggggca ggcctgccag gagagaccct
     1441 tgagagagcc gttggcgtcg gtgcagacgt ccttggtgta gccgtacatc gcggcggtgt
     1501 cgtcttccgt caggccgacg tcggtgtaaa catccagcgt gtaatcggtg ttgacatact
     1561 tgagggcata gtccgcttca atcaggaaca ggtcgatctt gtcgtcggcg gctgcggttt
     1621 cctgggcaag cagcgcttca tcgagcttgt tctggtaagc gttgtcggcg ttcggcgtga
     1681 tgacccagtt gatcgttacg ccgtccggca cgaggcccgc ttccacaaag tacttttcga
     1741 aacgggcctg gaactcgtcg ttccagcagt agatgttgta gacttttccc tgttcaaccg
     1801 gagccgctgc ttcggcggga gccgccgccg ttgcttcttc ggtcgtagct gcggtcgttt
     1861 ctgcgggttt cgagcaggcg cacacggaca acagtacagc cacagtcaat agaatcgcga
     1921 gtagtttttt cattgatact cctcctgttt attatttgaa cagacgcccc atcagcatcc
     1981 gttctatatt gagaacgcat cagcgcctca aaacttaatc ggtttacaga acggccttca
     2041 ttgagaacgc gcaagcgcct caaatattaa gcgtttttac cgaatggcct tccagcaggg
     2101 ttcccgatac ggtaaccgac tgcggaagcc agcttttcgg gttctcgatt tgctccacga
     2161 gcagcgccgc ggctttcctg cccatggtct ccgcgtcctg acggaaggtt gtgagctttg
     2221 gccgcagcac ctgagaaagc aggatgccgt cgtagccgac aacgctcata tcgtccggaa
     2281 tggaaagtcc ttgacgctcc agctcgttca tgccgccgat aaaggaaaaa tcgtcggggt
     2341 agaggatgca ggtcggccgc tccggaagcg cgagcagatc tcgcgtggca aggccgctcg
     2401 agcgcggatc gtggtagata gcagcgcgaa cataggcttc cggtatgcga aggtcgaact
     2461 cgctgcaggc cttaaagaag cttgcgatac gcttttgggt tacggaagtg tcttcgccgt
     2521 gaataaaggc gatgcggcgg tgaccgttgc tgtagacata ccgcacgatc t
//
LOCUS       DAOMGY010000194         2564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_44348_length_2564_cov_2.640893, whole genome shotgun sequence.
ACCESSION   DAOMGY010000194 DAOMGY010000000
VERSION     DAOMGY010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44348_length_2564_cov_2.640893"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PLR57_03830"
     CDS             <1..1098
                     /locus_tag="PLR57_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQQ40617.1"
                     /translation="ARFGLEADGKPVSKNRVYQLRDGLAEPIINKFYEDPTLTAFGED
                     VRDWGGAFAVYRGMTEAIPYNRLFNTPIAEAGIVGAAIGYAMSGGRAVAELMYCDFLG
                     RAGDEIFNQLCKWQSMSAGILKMPVVLRMSVGSKYGAQHSQDWSALVAHIPGLKVVFP
                     ATPYDAKGLMQSALNGTDPVIFLESQRIYDVAELFHSEGVPEAEYEIPFGEPDVKRVG
                     KDVTILSIGATLYRATKAADMLQEQYGLSAEIIDARSLVPFNYEPVIASVKKTGRIVI
                     TGDACERNSFMRNLASNIAELAFDYLDAPPVVVGAKNWITPAHELEDAFFPQPEWIVD
                     AIHERILPLPGHVCKHNFTTLEQIRENKRGI"
     gene            1219..>2564
                     /locus_tag="PLR57_03835"
     CDS             1219..>2564
                     /locus_tag="PLR57_03835"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQQ40618.1"
                     /translation="MKAIEQKAITNIRFLAAEAVQKAKSGHPGLPIGAAPMAFTLWKQ
                     MKHDPANPKWAGRDRFVLSAGHASMLEYSLLHLFGYGLPMDELKSFRQWGSLTPGHPE
                     FGHTVGVETTTGPLGQGVATAVGMAMAEARLAAEFNREEFPVVDHYTYVLSGDGCLME
                     GVASEAASLAGHLGLGKLILLYDRNQITIEGSTNLAFTEDVGKRFEAYGWTVQELPDG
                     EDIGAIDTAIQAAKADKTHPSLIIVHTEIAHGTPKANSASAHGEPLGEDAIKAMREFY
                     NWNEEPFTVPADVQAYFAELKTGYAKEHAAYDKMFAEYKKAYPELYEKWVAWHDKKMP
                     QALKTDTRLWNASGAKATRAVSGEVLNIFAEYLPNLFGGSADLAPSNKTELKGKGFFS
                     AENRTGANVHFGIREFAMACISNGIQLYGGFHAFCATFLVFTDYLKPALRLSAIMR"
BASE COUNT          588 a          739 c          732 g          505 t
ORIGIN      
        1 gctcgctttg gcttggaagc agacggcaaa cccgtcagca agaatcgcgt ctatcagctg
       61 cgcgacgggc ttgcggaacc aatcatcaac aagttctacg aggatccgac tctcacggcg
      121 ttcggcgaag acgtgcgcga ttggggcggc gcgttcgcgg tctaccgcgg catgacggaa
      181 gcgattccct ataaccgcct gttcaacacg cctatcgcgg aagcgggtat cgttggcgcc
      241 gcaatcggct acgccatgag cggcgggcgc gcggttgcgg aactcatgta ctgcgacttc
      301 ttaggcaggg cgggagacga aatctttaac cagctctgca aatggcagtc catgagcgcg
      361 ggtatactca aaatgccggt cgttctgcgt atgagcgtcg gcagtaagta cggcgcacag
      421 cactcgcagg actggagcgc tctggttgcg cacatccccg gcctgaaggt cgtgttcccg
      481 gcaacgcctt acgacgcaaa gggactcatg cagtccgcgc tcaacggcac cgatccggtt
      541 atcttcctcg aaagccagcg catctacgac gttgccgaac tcttccattc ggaaggcgtg
      601 ccggaagcgg agtatgaaat tccgttcggc gagccggatg ttaagcgcgt cggcaaggac
      661 gtgaccatcc tctccattgg cgctacgctt taccgcgcga cgaaggccgc cgacatgctg
      721 caggaacaat acggcctctc tgcggaaatc atcgacgcgc gttcgctcgt tccgttcaac
      781 tatgaaccgg ttatagcatc cgtcaaaaag accgggcgca tcgtcatcac cggcgacgca
      841 tgcgagcgca acagctttat gcgaaacctc gccagcaaca ttgccgaact ggcgttcgat
      901 tacctcgacg ctcctccggt cgttgtcggc gcgaagaact ggattacgcc tgcgcatgag
      961 ctggaagacg cgttcttccc gcagccggaa tggattgtgg acgcaattca cgagcggatt
     1021 ctgccgctgc cggggcacgt ttgcaagcat aactttacaa cattggagca gattcgcgaa
     1081 aacaagcgcg gtatctgatc cgggttaggt tatacggcgg caagtatgat ataatacttg
     1141 tcgccgtctt tatacatttt tctattcttt gttgattatt ctctgcctgt acaaacgaac
     1201 gaaaggaata taaaagacat gaaggcaatt gagcaaaaag caatcaccaa cattcgtttt
     1261 ctcgcggcgg aagccgttca aaaagcaaaa agcggccatc ccggcctgcc catcggcgcc
     1321 gctcccatgg cgttcacact ttggaagcag atgaagcatg atcccgccaa ccccaagtgg
     1381 gcggggcgcg accggttcgt cctctcggcc gggcacgctt ccatgctgga atactctctg
     1441 ctgcatctgt tcggttacgg tctgcctatg gatgaactca agagttttcg ccaatggggg
     1501 agcctgacgc ccggtcatcc ggaattcggc cacacagtcg gcgtagaaac gacaaccggc
     1561 ccgctggggc agggcgtcgc cacagcagtc ggcatggcga tggcagaagc gcggcttgca
     1621 gccgagttca accgcgagga gttcccggtt gtcgatcatt atacttacgt tctttccggc
     1681 gacggctgtc tgatggaagg cgtcgcttcc gaagcggctt cgctggcggg gcatcttggc
     1741 cttggcaaac tgattctgct gtacgaccgc aaccagatca ccatagaagg cagcacgaat
     1801 ctcgcgttta ccgaggatgt cggcaagcgg ttcgaagcgt acggctggac ggtgcaggaa
     1861 cttccggacg gcgaggatat cggcgcgatc gacacggcaa ttcaagccgc gaaagcggac
     1921 aaaacgcatc cttcgctcat catcgtgcat acggagattg cgcacggcac accgaaagca
     1981 aattccgcaa gcgcgcacgg cgaaccgctg ggcgaggatg cgatcaaagc catgcgcgag
     2041 ttctacaact ggaacgaaga accgttcacg gtacctgcgg acgtgcaggc atatttcgcc
     2101 gagctgaaaa cgggctacgc aaaagagcac gcggcgtacg acaagatgtt tgccgaatat
     2161 aaaaaagcct atcccgaact ctacgaaaag tgggtcgcgt ggcacgataa aaaaatgccg
     2221 caggcgctga aaaccgacac gcgcctttgg aacgcttccg gcgcaaaggc aacgcgcgcg
     2281 gtcagcgggg aagtgctcaa tatctttgcc gaatacctgc cgaacctctt tggcggcagc
     2341 gcggatctcg cgccttccaa caaaacggaa ctcaaaggca aaggattctt cagcgcggaa
     2401 aaccgaaccg gtgcgaacgt ccactttggc attcgtgaat tcgccatggc ctgtatcagt
     2461 aacggcatcc agctctacgg tgggttccat gcgttctgcg cgacgttcct cgtgtttacg
     2521 gattatctca aacccgcgct gcggctatcc gccatcatgc gcca
//
LOCUS       DAOMGY010000195         2562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_44391_length_2562_cov_3.039888, whole genome shotgun sequence.
ACCESSION   DAOMGY010000195 DAOMGY010000000
VERSION     DAOMGY010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44391_length_2562_cov_3.039888"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /gene="ftsZ"
                     /locus_tag="PLR57_03840"
     CDS             complement(<1..1152)
                     /gene="ftsZ"
                     /locus_tag="PLR57_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017811789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQQ40619.1"
                     /translation="MFEPEFENASFAQIKVIGVGGAGCNAINRMVQYGLRGVEFIAIN
                     TDKQALYLAKAPIKIQIGEKLTKGLGAGADPEVGRKAADESREMITEALRGTDMAFIT
                     AGMGGGTGTGATAIVAECARELGILTIAVVTKPFSFEGKVRMRNAEIGIAALKPAVDT
                     LITIPNDKLIDLVGRASLPDALRVADDVLRQGIQGISDLIAVPAMINLDFADVKTIMR
                     EKGLAHMGIGNASGEKRAAEAARQAIESPLLETSINGARGVLMNITGGPDLSLLEVNE
                     AAELIGENVDPEANIIFGADIDEAMGDALRITVIATGFDRTDRSGGAQVLPGYGTPFS
                     AYRPNYGGMGMGAQAPQSQPMQQQPQPMQQPIQQQPQYQPQQPMRQNPGQ"
     gene            complement(1165..2346)
                     /locus_tag="PLR57_03845"
     CDS             complement(1165..2346)
                     /locus_tag="PLR57_03845"
                     /inference="COORDINATES: protein motif:HMM:NF025806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HQQ40620.1"
                     /translation="MKPAAVLDIGSSKVVCLCGSFVNRDGITVHGVSVCPYHGYQGGT
                     FSDHRSLHNAIVEAVSHTEQEARLRIREIAVSVPAPFCKLVMTEAALPIGSRSKRVMA
                     EDIDDVISLSLKKVKLSGYVLMHSTPVSFTVGGVVSSTLPTGAKTEELGALVSHMYVR
                     EDFVKAIEQVLVTLDIEISMSVSSQLCGALAIIPEKERVRPAILIDVGYTHTDVSLVE
                     NAALTDMRTIEIGGKHFASDLAFGLDVPLETAEQVKRRYVFLQEPLSTTEIVRSPAGA
                     KRVDHAVIELILEARASELVSLLREAIKEMGVSPEASPVTYLTGGGLAMMKGGIDYLK
                     RGLQLSIQRDTPWVVDMDTPNFTSAFAALDFVLRATSDDVVTDTSPGTVVDRLKNLFT
                     K"
BASE COUNT          492 a          779 c          744 g          547 t
ORIGIN      
        1 ctggccggga ttctggcgca tcggctgctg gggctgatac tgcggctgct gctgaatcgg
       61 ctgctgcatc ggctgcggct gctgctgcat cggctgggac tgcggtgcct gcgcgcccat
      121 gcccatgccg ccgtagttgg gacggtatgc gctaaacggc gtaccgtacc cgggcagaac
      181 ctgcgcgccg ccgctgcggt cggtgcggtc gaacccggtt gcgataacag taatgcggag
      241 cgcgtcgccc atggcttcgt cgatatccgc gccaaagatg atattggctt cgggatcgac
      301 gttttcgccg atgagttctg cggcttcgtt gacttcgagc aggctcagat ccgggccgcc
      361 cgtgatgttc atgagcacac cgcgcgcgcc gttgatgctg gtttcgagca gagggctttc
      421 gatggcctgc cgcgctgctt ccgccgcgcg tttttcaccg gacgcgttgc cgatgcccat
      481 gtgggcaaga cccttttcgc gcatgatggt cttgacgtcc gcaaagtcga ggttgatcat
      541 tgccggaacg gcgatcaaat cggaaatgcc ctgaatgccc tggcgcagca cgtcgtccgc
      601 aacgcggagc gcgtcgggaa ggctcgctct gccaacaagg tcgatgagct tgtcgttcgg
      661 gatcgtaatg agcgtgtcca cggcgggctt gagcgcggcg atgccgattt cggcgttgcg
      721 catgcgcacc ttgccttcaa acgagaaggg cttggtcacg accgcaatgg tcagaatacc
      781 cagttcgcgg gcgcattcgg caacgatagc cgtggctccc gtgccggttc cgccgcccat
      841 accggcggtg atgaacgcca tatccgtgcc gcggagcgct tcggtgatca tttcgcggct
      901 ctcgtccgcg gctttgcggc cgacttccgg gtccgcgccc gcgccgaggc ctttggtcag
      961 cttctcgccg atctgaatct taatgggtgc ttttgccaaa tacagcgctt gcttatcggt
     1021 attaatcgca atgaattcaa cgcccctgag gccgtattga accatgcggt tgatcgcgtt
     1081 gcagccggcg ccgccgacgc cgataacttt tatctgggca aatgatgcgt tttcgaattc
     1141 cggttcaaac atagtttgtc ccccttattt ggtaaaaagg ttcttaagcc tatccactac
     1201 cgttccgggc gacgtgtcgg tcacgacgtc gtcgctcgtt gcgcgcagaa caaaatccaa
     1261 cgccgcaaaa gcgcttgtaa agttgggcgt atccatatcg acaacccacg gcgtatcgcg
     1321 ctgaatggac agctgcagac cgcgcttgag gtaatcgatt ccgcccttca tcatcgccag
     1381 gcccccgccg gtgaggtagg tcacggggct ggcttcggga gatacgccca tctccttgat
     1441 ggcctcgcgg aggagcgaaa ccagttcgct cgcgcgcgct tccagtataa gctcgatcac
     1501 cgcatgatcc acgcgctttg cgcccgcggg cgagcggaca atctccgtcg tactcagcgg
     1561 ctcctgcaag aacacatacc gccgtttaac ctgctccgcc gtctccagcg gaacgtcgag
     1621 gccgaaggca agatcggagg caaagtgctt gccgccgatc tcgatggtgc gcatatcggt
     1681 cagcgcggcg ttttccacga gggatacgtc cgtatgcgtg tacccgacgt cgatgaggat
     1741 cgccgggcgg acgcgttcct tttcgggaat aatcgccagc gcgccgcaga gctgcgagga
     1801 aacgctcatg gagatttcaa tatccagcgt cacaagcacc tgctcgatgg cctttacaaa
     1861 atcctcacga acatacatgt gggaaacgag cgcgccgagt tcctcggtct tcgcgcccgt
     1921 cggaagcgta gaagatacaa cccctcccac cgtaaatgaa acgggcgtgc tgtgcatgag
     1981 cacatatccg ctcagtttca cttttttgag ggagagcgag atgacgtcgt caatatcctc
     2041 cgccatcacg cgcttggagc ggctgccgat gggcagcgcg gcttccgtca tgaccagctt
     2101 gcagaaaggc gccggcacgc tgaccgcgat ttcgcggatg cgcagcctgg cttcctgttc
     2161 ggtatgggaa accgcctcta cgatcgcgtt gtggagggag cggtgatccg aaaacgttcc
     2221 gccttgataa ccgtgatacg ggcacaccga aacgccgtgc accgtaatgc catcccgatt
     2281 gacaaaactg ccgcacagac agacaacctt tgaactccct atgtccagta ccgcagccgg
     2341 cttcaacgcg gtctcctccg ccaacatctc aacatatacg gcgatattat atcacagcgt
     2401 aagaaaagcg cgcaaccaaa gtgcattgtt tcataattgt tcgttttttc gacatggttg
     2461 ccgctgtttc cccgtcgccg gacgaggagt tcgtccaacg cacaatttcc gctgtcaaaa
     2521 cgcgcgtccg tccgaaaatt caatgcgcct tgccctgaca aa
//
LOCUS       DAOMGY010000196         2562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_44394_length_2562_cov_2.932988, whole genome shotgun sequence.
ACCESSION   DAOMGY010000196 DAOMGY010000000
VERSION     DAOMGY010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44394_length_2562_cov_2.932988"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLR57_03850"
     CDS             complement(<1..627)
                     /locus_tag="PLR57_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HQQ40621.1"
                     /translation="MKILVINPGSTSTKLSLFEDDALLFEESEFHDAPVLLQYPSVND
                     QVPFRKKVILDIMQKNGYAPEDVDVFVGRGGSAYPQRAGVMPIDEWLYRDTWDAVGGS
                     EHAAKLGVLLAYELSREYGGQMFTLNPTNIDELCDYARVTGISGVYRNAQTHALNQKA
                     VARYHAESTGRRYEDCRFVVAHIDGGVTINAHDCGKMVDGNVGSGGDGA"
     gene            complement(874..1707)
                     /locus_tag="PLR57_03855"
     CDS             complement(874..1707)
                     /locus_tag="PLR57_03855"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQQ40622.1"
                     /translation="MIWVIIIAAFLLLLVCGALAFGNGFVNQRGVPRMTRRVRFGNDD
                     RILYVVAHPDDEVLMSGTLSLLKRSLKLPVKALYLTHGEDGPTFDLVPKSGLKERRTQ
                     ELGRVKEILSLDEMVVCDYPDRYLANQDFEEVCRTVGREMEAFQPTYVFTFDESIGMY
                     GHTDHVAAGKAAVRMAKQAESVKGVFQMTLPSGMLALALKMSKTFRERYNVEHGLPKP
                     NVAVNIYRWRKYRYAVTRAHETQTAVMRELQPYYDKLPRWLYYAVFDREYFYYRDKSE
                     L"
     gene            complement(1704..>2562)
                     /locus_tag="PLR57_03860"
     CDS             complement(1704..>2562)
                     /locus_tag="PLR57_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ40623.1"
                     /translation="FVQSFVSVFGILGMGVALGASGFLINALGYLGAGVALSVICALV
                     MVVPALVIKVNPDYVPAPAPNQTKNLFKNIVGAFKNDKFVKYIIGFATFFIGFQLVQY
                     NLAFVTTVSLGLEKGMSSTLFIVSVVCALAFIPLYNVFVKKFGTVKSLMISMASYALV
                     ALLIMSLPQIKGIPGTALGFALMALLGFPYSGLMVIPNVLVSEIIDDDYKRFAVRREA
                     MFFGVQGLVNKLATAFASFAVGVLHDLLGSTATQSLGITVVAPIAAVFSLFGFFVMLR
                     LNREKAAQQ"
BASE COUNT          598 a          752 c          671 g          541 t
ORIGIN      
        1 agcgccatcc ccgccggaac ctacgtttcc atccaccatt ttgccgcagt cgtgcgcgtt
       61 gatcgtaacg ccgccgtcga tatgcgccac cacgaagcgg cagtcctcat accgtctgcc
      121 cgtcgattcc gcatggtaac gtgcgaccgc cttttggttg agcgcgtgcg tctgggcgtt
      181 gcggtacacg ccggaaatgc cggtcacgcg cgcgtaatcg cagagttcgt cgatattcgt
      241 cgggttgagc gtaaacatct gcccgccgta ttccctggag agttcatagg ccagcagcac
      301 gccaagtttt gccgcgtgct cgctgccgcc gaccgcgtcc caggtatcgc ggtaaagcca
      361 ttcgtcgatc ggcatgacgc ccgcgcgctg cggataggcg cttccgccgc gtccaacaaa
      421 aacgtctacg tcttcgggag catagccatt cttctgcata atatccaaaa tcaccttctt
      481 gcggaaggga acctgatcgt tcaccgaggg gtactgcagt aaaaccggag cgtcgtgaaa
      541 ctcgctctcc tcaaacagca aagcgtcgtc ttcaaaaagg ctgagtttag tggatgtgga
      601 gcccgggtta atgaccagaa tcttcatcag agtaatatcc tttcgaacct gcgggggatt
      661 tggggctggt tgggcatccc aacaatcgtc aattatagca tgtcgagcga tttcttcaat
      721 attaaacaac attttcatgc gaaatttctg catgtttact gcatatttca tcccgatgcg
      781 taacattatg aacctgtaaa cgccaaaaca aaaaagaggt tcgttcgaac ctcttttttg
      841 tatcgttcta tcgtattttg acagatgttg tgtctacagc tcgcttttat cgcgatagta
      901 gaagtactcg cggtcgaaaa cggcgtaata gagccatcgc ggcagtttgt cgtaatacgg
      961 ctgcagttcg cgcatcaccg ccgtctgcgt ttcgtgcgcg cgcgtgactg catagcggta
     1021 tttccgccaa cgatagatgt ttacggcaac attcggcttt ggcaatccat gctcgacgtt
     1081 gtaacgctcg cggaaggtct tgctcatctt gagcgcaagc gcaagcatgc cgctcggcag
     1141 cgtcatctgg aacacgcctt tcacgctctc agcctgcttg gccatgcgga ccgccgcttt
     1201 ccctgcggca acatggtcgg tatgcccgta catgccgatg ctttcgtcga aggtgaatac
     1261 atacgttggc tgaaaggctt ccatctcgcg gccgaccgtt cggcagactt cttcgaaatc
     1321 ttgattcgcc agatagcggt cgggatagtc gcaaacgacc atttcgtcta gcgaaagaat
     1381 ctcttttacc cgcccgagtt cctgcgtacg ccgctctttc agcccgcttt tcggcacgag
     1441 atcgaaggtc gggccgtcct cgccatgcgt caggtaaagc gccttgaccg gcagcttgag
     1501 cgaccgcttc agcagcgaca gcgtgccgct cataagcacc tcgtcgtccg gatgcgcaac
     1561 gacataaaga atccggtcgt cgttaccaaa gcgcacccgc cgcgtcatgc gcggtacgcc
     1621 ccgctggttg acaaacccgt tgccaaacgc cagcgcgccg caaaccagca gcagaaggaa
     1681 tgccgcaatg ataataaccc agatcactgc tgcgccgcct tctcacggtt cagccgcagc
     1741 atcacaaaga agccgaacaa tgaaaacacc gccgcaatgg gcgcaacgac cgtgatgccg
     1801 agggactgcg ttgccgtgct gcccagcagg tcgtgcagaa cgccgaccgc gaacgaggca
     1861 aacgcggttg caagcttgtt gacgagaccc tgcacgccaa agaacatcgc ctcccggcga
     1921 accgcaaagc gcttgtaatc gtcgtcgatg atttccgaaa cgagcacgtt gggaatgacc
     1981 ataagcccgc tgtacggaaa gcccagcagc gccatcagcg caaaaccgag cgccgtaccg
     2041 ggaatgcctt tgatttgagg caatgacata atgagcagcg cgaccagcgc gtagctcgcc
     2101 atggagatca tcagcgactt gactgttccg aacttcttga caaacacgtt gtagagcgga
     2161 ataaacgcca gcgcgcagac cacggagacg ataaagagcg tgctggacat tcccttctcc
     2221 aggccgagcg aaaccgttgt aacgaacgca aggttatact gcaccagctg gaagccgatg
     2281 aagaacgttg caaacccgat gatgtatttt acgaatttgt cgttcttgaa cgcgccgacg
     2341 atgttcttaa agagattctt cgtctggttc ggcgcgggtg cgggaacgta atccgggttg
     2401 actttaatca ccagcgcagg cacaaccatc actagggcgc aaataacgct cagcgccaca
     2461 cccgctccga gatatcccag cgcgttgatc aaaaagcccg aggcaccaag cgcaacaccc
     2521 atgccgagaa tgccgaacac ggagacaaag ctctgcacga at
//
LOCUS       DAOMGY010000197         2557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_44544_length_2557_cov_2.349321, whole genome shotgun sequence.
ACCESSION   DAOMGY010000197 DAOMGY010000000
VERSION     DAOMGY010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44544_length_2557_cov_2.349321"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PLR57_03865"
     CDS             <1..767
                     /locus_tag="PLR57_03865"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HQQ40624.1"
                     /translation="LMTSVPGIFSCGNGLHVHDIVDMVSNEAELAAKNAVEFLKHSLP
                     KQPQIVVLAESGVRYVLPRSLPQNAPSHLSLRVQNPGTDQTVLVTQGGKTLLKKRMRR
                     LSPAEMIRLSLPAVRANAGELRVSLRQDPQSPAREKSDLICTCCPNGCSLTAVTNTKG
                     KLEITGAICSKGVSFGLSELTDPRRTLTTTVRLENGAYPLVAVRSAEPVKKTDLRRLV
                     AGLRSVTLTAPVRCGDRVPFDEAEILVSGRAEAVKK"
     gene            complement(830..1270)
                     /locus_tag="PLR57_03870"
     CDS             complement(830..1270)
                     /locus_tag="PLR57_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40625.1"
                     /translation="MNTVVLYYTFGGSTKKEAEKIGSELGAPVYRVKEKRNRSLIGSF
                     IPGGFQAMKRKKTKILPLEVDLNAFDRILIGCPVWAACPAPAFNSIVDLLPAGKEVEV
                     FLCSGGNDARPSDDVTKSMIEAKGCKVTGIRTIATGVAPSKMKE"
     gene            complement(1422..2090)
                     /locus_tag="PLR57_03875"
     CDS             complement(1422..2090)
                     /locus_tag="PLR57_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQQ40626.1"
                     /translation="MKILVINGSPRGEKSVTLKITRAFLEGMGESAEIIDTMQVNVKP
                     CLGCLSCWWKTPGVCAQKDDMADILKRLLESDLVIWSVPLYCYNVPSNCKVLYDRLIP
                     LGTPAQEVDETGTTYHPGRQEIHTKIMLISSCGFPDFQHNFDGMDFQFNRMFSKDCPR
                     IYCVEAPLLNVPEAKPLADAYLAAAKRAGAEYKANGVISAETKATLDAPMLPPDVYRA
                     QIKR"
     gene            complement(2218..>2557)
                     /locus_tag="PLR57_03880"
     CDS             complement(2218..>2557)
                     /locus_tag="PLR57_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HQQ40627.1"
                     /translation="RFYVCPVCGNVICAMGEGAFSCCGVTLPPLETEENDEAHEIYVE
                     PVENERYVTMRHPMEKTHYISFFAFMTSDRVQIKKLYPEMDAEARFPAGTHGIFYAFC
                     NRHGLYAKRI"
BASE COUNT          526 a          758 c          707 g          566 t
ORIGIN      
        1 gcctcatgac cagcgtcccc ggcatatttt cctgcggcaa cggcctgcat gtgcacgaca
       61 ttgtggacat ggtttccaac gaagcggaac tcgcggcaaa gaacgccgtg gaatttttga
      121 agcattccct gcccaaacag ccgcagatcg tcgtgctcgc ggaaagcggt gtgcggtacg
      181 ttctcccgcg cagcctgccg cagaacgcgc cgagccatct atcgcttcgc gtgcagaatc
      241 cgggaaccga tcaaacggtg ctggtcacac agggcggcaa aacactcctg aaaaagcgga
      301 tgcggcggct ttcccccgcc gagatgattc gcctttcgct ccccgctgtc cgagcaaacg
      361 ctggtgaact ccgcgtctcc ctccggcagg acccgcagtc ccccgcacgg gaaaagagcg
      421 atttgatctg cacctgctgc cccaacggct gttcgctcac cgcggtcacg aacacaaagg
      481 gaaagctgga gatcacaggc gcaatctgca gcaagggcgt cagctttgga ctaagcgagc
      541 tgaccgaccc ccggcggacg ctgaccacga cggtgcgcct cgaaaacggc gcatacccgc
      601 tcgtagccgt ccgcagcgcc gaaccggtta agaaaactga ccttcgccgc cttgtagcag
      661 gtcttcgctc tgtcacgctg accgctcccg ttcgctgcgg cgacagggtg ccgttcgacg
      721 aggcggaaat cctcgtaagc gggcgagccg aagccgtgaa aaagtaaaac cgaatccata
      781 caaaaaacgc tccgaaaaaa cggagcgttt tcttatgtcc ctatacgtgc tattccttca
      841 tcttggacgg agccacgccc gttgcgatgg tgcgaatgcc ggttaccttg cagcccttcg
      901 cctcgatcat cgacttggta acatcgtcgc tcggacgcgc gtcgtttccg ccggagcaga
      961 gaaagacttc cacttccttg ccagcgggca gcagatccac aatggagttg aacgcgggcg
     1021 cgggacatgc cgcccagacc gggcagccga tcaggatgcg gtcgaacgcg tttaagtcca
     1081 cttccagcgg cagaattttc gtctttttgc gcttcatggc ctggaatccg ccgggaatga
     1141 acgagccgat caggctgcga ttcctctttt ctttcacgcg gtagaccggc gcgccgagtt
     1201 cgctgccaat cttctccgct tcctttttgg tggagcctcc gaacgtataa tacaaaacga
     1261 ccgtgttcat tgatgcttct cctttgtttt gtgcgtagtt cgcgggctta tatccgctct
     1321 gttcgccgga ttcattctat catacctgca aaaatgcgca agaaaaaaac cggcgaaacg
     1381 cggttgttcc gcattcgccg gtcttgtatg gaatatcgtt ttcagcgctt gatctgcgcg
     1441 cggtaaacgt ccggcgggag catcggcgcg tcgagtgtcg ccttcgtttc agcggaaatc
     1501 acgccgtttg ccttgtactc cgcgcccgcg cgtttcgccg cggcaaggta cgcgtccgcg
     1561 agcggctttg cttcgggaac gttcaaaagc ggcgcttcca cgcagtagat gcgcggacag
     1621 tccttgctga acatgcggtt gaactgaaag tccatgccgt caaagttatg ctggaaatcc
     1681 gggaatccgc agctagaaat cagcataatc tttgtatgga tttcctgcct gccggggtgg
     1741 taggtcgtgc cggtttcgtc cacttcctgc gcgggcgtac caagcggaat cagacggtcg
     1801 tagaggactt tgcagttgga aggcacattg tagcagtaca gcggaaccga ccagatgacg
     1861 aggtcgcttt ccagcagccg cttgagaata tccgccatat cgtccttctg ggcgcagacg
     1921 cccggcgttt tccaccagca ggaaaggcag ccaaggcagg gtttgacgtt cacctgcatc
     1981 gtgtcgatga tctccgcgct ctcgcccatg ccttcgagaa acgcgcgggt aattttgagc
     2041 gtaacgctct tttcacctct cggcgagccg ttgatgacca gtatcttcat aaagttttcc
     2101 tttcaattcg tttgttccat tcattctgtg cgcgccgcaa acaagcggca cgttctataa
     2161 aaaagcctgc tgcagcgaac ttttttccgt tttgtatggg taaaatccgg aatccgatta
     2221 gattctcttt gcgtaaagcc cgtggcggtt gcagaatgcg tagaagattc cgtgcgtgcc
     2281 cgccggaaac cgcgcttccg cgtccatctc cgggtagagc tttttgatct gcacccggtc
     2341 ggacgtcata aacgcgaaaa acgaaatgta gtgcgtcttc tccatagggt ggcgcatggt
     2401 gacatagcgc tcgttctcca cgggttccac gtagatttca tgcgcttcgt cgttttcctc
     2461 cgtttccagc ggcggaaggg tcacgccgca gcaggaaaac gcgccttcgc ccatggcgca
     2521 gatgacgttt ccgcagaccg ggcagacata gaagcga
//
LOCUS       DAOMGY010000198         2554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_44615_length_2554_cov_3.378952, whole genome shotgun sequence.
ACCESSION   DAOMGY010000198 DAOMGY010000000
VERSION     DAOMGY010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44615_length_2554_cov_3.378952"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLR57_03885"
     CDS             <1..794
                     /locus_tag="PLR57_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQQ40628.1"
                     /translation="MGEVDVYKQAPNVTRMKMLGARVIPVKEGLRTLKEAVDAAFMAY
                     MSEYEDAIYCIGSVVGPHPFPKMVRDFQMVVGIEAKDQFKEMTGFLPDIVTACVGGGS
                     NAAGLFIPFLEEPVEIVGVEPMGRGTAVGDNAASITFGKKGVLHGFESLLLQDEAGAP
                     LPVYSIASGLDYPSVGPEHAFLHDVGRVKYEYVTDDEAMEAFFLLCRNEGIIPAVESS
                     HALAYAIRYARSNAKGSLLVNLSGRGDKDIDYIFEHYGTGEQFFE"
     gene            1129..>2554
                     /gene="glmS"
                     /locus_tag="PLR57_03890"
     CDS             1129..>2554
                     /gene="glmS"
                     /locus_tag="PLR57_03890"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HQQ40629.1"
                     /translation="MCGIVGYTGEKNCVPVLMDGLTALAYRGYDSSGLAVWEGSELSL
                     RKAKGKIENLNTLLKSSPISGKCGIGHTRWATHGEPSDVNSHPHTDSGKTIALVHNGI
                     IENYLKLKQMLQSRGCVFVSETDTEVVAQLLGYLYRGNPLSALKQAIALIEGSFALAI
                     IFADDPETIYCTCKDSPMVVGHGEGESVVASDIPAILAHTRDVCFVQDRQIAVLKKDG
                     ICYYDEFGVAIEKTPTHVDWDVESAKKGNHAHFMIKEICEEPDAFRRTFEQYVSVKEH
                     TLKSDRFPWDAERAKSLDRLTIVSCGTAYHAAMLGKKFIEHLAKLQVDVDIASEFRYG
                     DTLLKKGEPFMVISQSGETADTIAAMRRAKELGCETVAVCNVTGSTISREAGHVLYTY
                     AGPEIAVAATKTYLTQIIVMYLLALDLAEKRGTMTHEEVSARLAEMAKIPASMQKLLD
                     EKEKIQFFASRSFAVKHVFFIGRGL"
BASE COUNT          597 a          671 c          753 g          533 t
ORIGIN      
        1 acatgggcga agtggacgtt tataagcagg cgcccaacgt cacccgcatg aagatgctcg
       61 gcgcgcgcgt catccccgtc aaagaaggcc ttcgcacgct caaggaagcg gtggacgcgg
      121 cgttcatggc ttacatgagc gaatatgagg acgctatcta ctgcatcggc tccgtggtcg
      181 gcccgcaccc gttccccaag atggtgcgcg atttccagat ggtggtcggc atcgaggcca
      241 aagaccagtt caaggaaatg accggatttt taccggatat cgtaaccgcc tgcgtcggcg
      301 gcggttcgaa tgcggcggga ctgttcattc cgttcctcga agagccggtg gagatcgttg
      361 gcgtagaacc catggggcgc ggcaccgcgg ttggcgacaa cgcggcttcc atcacgttcg
      421 gcaaaaaggg cgtgctgcac ggctttgaaa gcctcttgct tcaggacgaa gcgggcgcgc
      481 cgctgccggt ctacagcatt gcaagcggtc tggattatcc ttccgtcggg ccggaacacg
      541 cgtttctgca cgacgtcggc agagtaaaat acgagtacgt aacggacgac gaagcgatgg
      601 aagcgttctt cctgctctgc cgcaacgagg gaatcattcc cgccgtggag agctcgcatg
      661 cgctggcata cgccatccgg tacgcgcgtt ccaacgccaa gggatcgctg cttgtcaacc
      721 tttccggcag aggcgataag gacatcgatt acatcttcga gcactacggc accggcgaac
      781 agttctttga ataaggggcg tttcaccgtt cataaaagcg gagttggctt gaaaaaggca
      841 ttgtgaactt attaagtcgc aatcgtctgg tacaataatc gaatgaattc gcgtaatccc
      901 gcaggactat cgatggcgcg gcgggagtaa aggttcgata caaaacacaa aatagggaaa
      961 tgtgacttcg gagcggccgt atatattgcg gtcgctccga aaattagctt gacgcaccga
     1021 gttgtaattt tgtataatga cacaggattt ttcgtccggt aatgggtggg cggttggcgg
     1081 cttattatcg gacgcttcat ttttggcaaa gggggagatt caacttacat gtgcggtatc
     1141 gttggttata cgggcgaaaa gaattgtgtt cccgtgttga tggacggcct gacggcgctt
     1201 gcctaccgcg gatatgattc ctcaggactt gccgtttggg aaggaagcga actctccctg
     1261 cgtaaagcga aagggaagat cgaaaacctc aacaccctgc tcaagagttc cccgatctcc
     1321 ggcaaatgcg gcatcggcca cacgcgctgg gcgacccacg gcgaaccttc ggacgtgaat
     1381 tcccacccgc atacggacag cggtaaaaca atcgcgctcg tccacaacgg cattatcgaa
     1441 aattatctta aactcaaaca gatgctgcag agccgcggct gcgtgttcgt ttccgaaacg
     1501 gataccgagg tggttgcgca gctgcttggt tatctttatc gcgggaatcc gctttccgcg
     1561 ctcaaacagg cgattgcgct catcgaaggc tcgttcgcgc tggcgattat ctttgccgac
     1621 gatccggaaa cgatttactg cacctgcaag gacagcccca tggtggttgg tcacggcgag
     1681 ggcgaaagcg ttgtcgcttc cgatatcccc gccattctgg cgcatacgcg ggacgtgtgc
     1741 tttgtgcagg acaggcagat tgcggtgctc aaaaaagacg gcatctgcta ttacgatgag
     1801 ttcggcgttg ccattgaaaa aacgccgacg cacgtcgatt gggacgtgga gtccgccaag
     1861 aagggcaacc acgcgcactt catgatcaag gaaatctgcg aggagcccga cgcgttcaga
     1921 agaacgttcg agcagtatgt ttccgttaag gagcatacgc ttaaaagcga ccgtttcccc
     1981 tgggatgcgg aacgcgcaaa gagcctcgat aggcttacga tcgtgagctg cggcacggca
     2041 taccacgcgg ccatgctcgg caaaaagttc atcgagcatc tggcaaagct gcaggtggac
     2101 gtggatattg cgagtgaatt ccgctacggc gacacgctgc tgaaaaaagg cgagccgttt
     2161 atggtaatca gtcagtccgg cgaaacggcg gataccattg ccgctatgag gagagccaaa
     2221 gaactcggct gcgaaaccgt cgcggtttgc aacgtcaccg gcagcacgat ctcgcgcgaa
     2281 gcggggcatg tgctgtatac ctacgccggg ccggagattg cggttgcggc aaccaagacg
     2341 tacttaacgc agattatcgt catgtacctg ctcgcgctcg accttgccga aaaacgcgga
     2401 accatgacgc atgaagaagt ttcggcgcgt cttgccgaaa tggcgaagat tcccgcgtcc
     2461 atgcagaaac tgctggacga aaaagaaaag attcagttct tcgcgagccg ctcgtttgcg
     2521 gtcaagcacg tgttctttat cggacgcggg ctgg
//
LOCUS       DAOMGY010000199         2552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_44674_length_2552_cov_3.195034, whole genome shotgun sequence.
ACCESSION   DAOMGY010000199 DAOMGY010000000
VERSION     DAOMGY010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44674_length_2552_cov_3.195034"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLR57_03895"
     CDS             complement(<1..496)
                     /locus_tag="PLR57_03895"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HQQ40630.1"
                     /translation="MEDELSSRIEELRSVLSRSKRIAFLGGAGTSTESGIPDFRSEKG
                     VFEAVRRFGHKPEVLLSHDFFLSHPDVFFEYYRDFLLYPNAKPNDAHRALASLERAGK
                     LGAVITQNIDGLHQKAGSKNVLELHGNVNRNFCMNCKKSFSMRDILAKSGVPKCDCGG
                     MIKPD"
     gene            647..1912
                     /locus_tag="PLR57_03900"
     CDS             647..1912
                     /locus_tag="PLR57_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQQ40631.1"
                     /translation="MKIRFFNARILPLDGATPLFTGELVVDGGKIVSLGEKSADIGPF
                     DREIDCNGNLLMPGFKNAHTHSGMTFLRSLADDLPLQRWLTEQVFPKEAQLTPEDVYV
                     LTKLAILEYLTSGITTMFDMYFARDSIAQAAIDTGFRACLVGCANDYGGTAEETVQEY
                     ERFSALHPRIAYQLGFHGEYTTSYPLLKDLSDMVHSLKLPFYTHLNETAAEVESCVKH
                     SGMRTIEYLDSLGLFDFGGGGYHCVHVSESEMDILKKRNMIVVSCPGSNTKLASGVAP
                     ITEYLSRGIPVALGTDGPASNNCLDFFREMFLVTGLQKLRCGAEAVDAMEVLNMACRN
                     GAYACGFPDCDCLAVGKQADLILIDLHQPNMQPLNNIEKNVVYSGTKQNVVLTMIAGS
                     ILYERGEFHIGDDPERIYAEANRIIRRMQ"
     gene            1979..>2552
                     /locus_tag="PLR57_03905"
     CDS             1979..>2552
                     /locus_tag="PLR57_03905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ40632.1"
                     /translation="MTDLKKIAPLFEGWEETMIWSCLDGSMGHALCDNEANPASAQII
                     LGDLCFFAGAPNDELSLKAGSPFLVPQSEAWCESIERAWGERVQKRLRYSIKKEPGVF
                     NTVKLACYANSLPDRFSLRLFDEVIVRESFEQSWSRDFCAQFADAADFLARGIGVAAL
                     YEGKLVAGASSYSVYRGGIEIEIDTKPEFRR"
BASE COUNT          557 a          733 c          697 g          565 t
ORIGIN      
        1 cgtccggttt aatcataccc ccgcagtcac atttcggcac gccggatttt gcgaggatgt
       61 cccgcatgga gaacgacttt ttgcagttca tgcagaagtt gcggtttaca ttaccgtgca
      121 gttccagcac gtttttgctg cccgccttct gatgcaggcc gtcgatattc tgcgtaatca
      181 ccgcgccgag tttgccagcg cgctccagac tcgccagcgc gcggtgcgcg tcgttcggtt
      241 ttgcgtttgg atacaaaaga aaatcgcggt aatactcgaa gaatacgtcc ggatggctca
      301 gaaaaaagtc atggctcaac aggacctccg gcttgtggcc gaagcgtcgc accgcttcga
      361 aaacgccttt ttccgagcga aaatccggta tgccgctttc cgtagatgtg cccgcaccgc
      421 ccagaaaggc gattcgcttg gagcgtgaga ggacgctcct caactcttcg atccgacttg
      481 atagttcgtc ttccatactt catattatag taagatacgg tgaatttgca agcgttcaaa
      541 gggttctctg aacaggaaat tttgccgccg ttccgctttt agctaggctc atcgccaaag
      601 ccgattttct gtggaaaccg ctcctgccgt atggtacaat acgcttatga aaattagatt
      661 ttttaacgca cgcatcctgc cgctcgacgg agcaacgccg ctgtttaccg gtgaactcgt
      721 cgtcgatggc ggaaagatcg tctctcttgg cgaaaaaagt gcagatatcg gcccgttcga
      781 ccgggaaatc gactgcaatg gcaatctttt gatgccggga ttcaaaaacg cgcatacgca
      841 ttccggcatg acgtttttgc gttcactggc ggacgatctg cctttgcagc ggtggctcac
      901 cgagcaggtt tttcccaagg aagcgcagct cacgccggaa gacgtatacg tgctcaccaa
      961 gctcgccata ttagagtatc tgaccagcgg cattacgacg atgttcgata tgtattttgc
     1021 gcgggacagc attgctcagg ccgccatcga cacgggtttc cgcgcgtgcc ttgtgggctg
     1081 cgccaacgat tacggcggaa ccgccgagga gacggtgcag gagtacgagc gcttctccgc
     1141 gcttcatccg agaatcgcct atcagctcgg cttccacggg gagtacacaa cgagctatcc
     1201 gctgctgaaa gacctttcgg atatggtgca ttccctcaag ctgccgttct atacacacct
     1261 aaacgagacg gcggcggagg ttgaatcctg cgtcaagcac agcggcatgc gcacgatcga
     1321 atacctggat tccctcggcc tgttcgattt cggcggcggc gggtaccact gcgtgcacgt
     1381 gagcgagagc gaaatggata tcctcaaaaa gcggaatatg atcgtcgtat cctgccccgg
     1441 ctccaacacg aagctcgcca gcggggtcgc ccccattacc gaatatctct cgcgcggcat
     1501 tccggttgcg ctcggcaccg acggccctgc cagcaacaac tgcctcgact tcttccgcga
     1561 gatgttcctc gtcaccggcc tgcagaagct ccggtgcggc gcggaagcgg tggacgccat
     1621 ggaggtgctc aacatggcct gccgcaacgg cgcgtacgcc tgcggtttcc cggactgcga
     1681 ctgcctcgcc gtcggcaaac aggcggatct gattttaatc gacctgcatc agccgaacat
     1741 gcagccgctc aacaacattg aaaagaacgt cgtctacagc ggcacaaagc aaaacgtcgt
     1801 gcttacgatg atcgctggca gcatcctcta cgaacgcggc gagttccata ttggcgacga
     1861 cccggaacgc atctacgcag aggcaaaccg catcatccgg cgcatgcagt aacgcatctg
     1921 aaacgccgct tcggcggcgt ttttctcttt tcagaaggta aatacatcag gagatttcat
     1981 gacagacttg aaaaaaattg ccccgctgtt cgaaggctgg gaagaaacga tgatttggtc
     2041 ctgcctggac ggttccatgg gacacgccct ttgcgataac gaagcgaatc ctgcctccgc
     2101 acagattatt ttgggcgatt tgtgtttctt tgccggagcg ccaaacgatg aattgagcct
     2161 gaaagcgggt tccccgtttc tcgtaccgca aagcgaagca tggtgcgaaa gtattgagcg
     2221 cgcatggggc gaacgcgtac agaagcggct tcgttattcc atcaagaagg aacccggcgt
     2281 gttcaacacc gtaaagctgg cctgctacgc caactccctg cccgacagat tctccctgcg
     2341 cctgttcgac gaggtgatcg tccgcgaatc gtttgagcag tcctggtcgc gggatttctg
     2401 cgcacagttt gccgacgcag ccgacttcct tgcgcgcgga atcggagtag cggcgcttta
     2461 tgagggcaag ctcgtggcag gcgcttcatc ctattcggtc taccgcggcg gcatcgaaat
     2521 tgaaatcgac accaagccgg agttccgcag gc
//
LOCUS       DAOMGY010000200         2545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_44873_length_2545_cov_3.090763, whole genome shotgun sequence.
ACCESSION   DAOMGY010000200 DAOMGY010000000
VERSION     DAOMGY010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44873_length_2545_cov_3.090763"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PLR57_03910"
     CDS             <1..312
                     /locus_tag="PLR57_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQQ40633.1"
                     /translation="ALRARGYLIPEQVSVIGFDDSYLATTVDPQLTTISHPKEVMGRH
                     TAQALVKLMKKQIRPPYIEIFRPELVKRGSCTRVFSGGTDPSEQRRKSVPAEQSADLD
                     E"
     gene            complement(381..1424)
                     /locus_tag="PLR57_03915"
     CDS             complement(381..1424)
                     /locus_tag="PLR57_03915"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HQQ40634.1"
                     /translation="MSVTKTLFGKMPGGEDVHLYTITNRSGASASILDYGATWQSMRV
                     PNRAGELVDVVLGYDTLEEYRKHTMFLGSTVGRVANRIGKSRFTLNGVEYKVFANDFE
                     NCCHGGREGFDKKIWKAEAEEDAVAFSLFSPDGDENFPGSLSVMVVYSLTEDNMLSIR
                     YFAVTDADTIVNLTNHAYFNLAGQGSGTILDQTLQVEADFATRIDSHILPTGEIVPVA
                     GGPLDFRKAKPIGQDIESTLENMGLAHGYDHNFVLSHREGGMERAATAADPVGGIVMD
                     TITDQPGVQLFTPTDFTGMRSKGGIEYGVRPGFCLETQHFADAMSHPEFPSIVLHAGE
                     IYSTETVYAFRVE"
     gene            complement(1474..2097)
                     /locus_tag="PLR57_03920"
     CDS             complement(1474..2097)
                     /locus_tag="PLR57_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40635.1"
                     /translation="MRLMNKELLLDQCPVLWHKELTEDVLREDFDIRSGQWHVEDGWL
                     YGENRGNFPGMVISRKDFYGNVLLEFTANTVSPSTHDINAMWNGSWNEQTNTRDVAYV
                     GGLEGWWHGKVGFEKSPDYKLNAANPLFDFHPGREYHIQMGSVDGHAFIIIDGALVLE
                     VTDPDPIDTAKYGRFGFEAYCSSIRIRDVYLRRAVAKAVEERYEPEF"
     gene            complement(2252..>2545)
                     /locus_tag="PLR57_03925"
     CDS             complement(2252..>2545)
                     /locus_tag="PLR57_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HQQ40636.1"
                     /translation="ERIIATGGGAKSALWCQLQADITGIPVSIPAEKEAACLGAAMIG
                     ATSAGVYKDFSEAANAAVRLEATFAPQADERNERKHRQFVALYEAMIGVQRMK"
BASE COUNT          517 a          700 c          768 g          560 t
ORIGIN      
        1 gcgctgcggg cgcgggggta tctgattccc gaacaggtgt ccgtaatcgg ctttgacgat
       61 tcgtatctgg cgaccacggt cgatccccaa ctgaccacga tttcccaccc aaaggaagtc
      121 atggggcggc ataccgcgca ggcgctggtc aagctgatga agaagcagat caggccgccg
      181 tacattgaaa tttttcgtcc cgagctcgtc aaacgaggct cctgcacgcg cgttttttcg
      241 ggaggaaccg atccttccga gcagcggcga aagtccgttc cggcggaaca aagcgcggat
      301 ttggatgagt aagtaaacaa aaaaactgcc cgtgaatgcg agcagttttt atgtacggat
      361 gtttactcaa tagatagtgc ttattcgacg cggaaagcgt acacggtttc ggtggagtag
      421 atttcgcccg cgtggagcac gatgctgggg aactcgggat gcgacatcgc gtctgcaaag
      481 tgctgcgttt ccaggcagaa tccgggacgg acgccgtatt caatgccgcc cttgctgcgc
      541 atgccagtaa agtcggtcgg cgtaaagagc tgcacgcccg gctggtcggt aatcgtgtcc
      601 atcacaatgc cgcccacggg gtcggctgcg gttgcggcgc gttccatgcc gccctcgcgg
      661 tggctgagca caaagttgtg gtcgtagcca tgcgcgagac ccatgttttc cagcgtggat
      721 tcgatatcct gcccgatggg ttttgccttt ctaaaatcga gcgggccgcc cgcgacaggc
      781 acgatttcac ctgtggggag gatatgcgaa tcgatgcgcg ttgcaaaatc cgcctcgacc
      841 tgcagcgttt gatcgagaat cgtgccgctg ccctgaccgg caaggttgaa atacgcgtga
      901 ttggtcagat tgacgatggt gtccgcgtcc gtaaccgcaa agtagcggat ggagagcatg
      961 ttgtcctcgg tgagcgaata gaccaccatg acggagagac tgccggggaa gttttcgtcc
     1021 ccgtccgggc tgaagagcga aaaggcgacg gcgtcttctt cggcttccgc tttccaaatc
     1081 tttttatcaa agccttcgcg tccgccgtgg cagcagtttt caaagtcgtt ggcaaagacc
     1141 ttgtactcca cgccgttgag cgtaaagcgg gacttgccga tgcggttggc gacgcgcccg
     1201 acggtagagc caagaaacat ggtgtgcttg cggtactctt ccagcgtgtc gtaaccgagc
     1261 acaacgtcca ccagttcgcc cgcgcggttg ggtacgcgca tgctctgcca ggttgcgccg
     1321 tagtcgagaa tggaagcgga cgcgccggag cggttcgtga tggtgtagag atggacgtct
     1381 tcgccgcccg gcatcttgcc gaagagggtt ttggtaacgc tcataatcgg ggtgcctcct
     1441 gttatgtaag tagtttttct gctgagataa agtttagaac tccggctcgt atcgttcttc
     1501 gaccgctttt gcgaccgcgc gccggagata tacgtcccgg atgcggatgc tggagcagta
     1561 cgcctcgaac ccgaaccgcc cgtatttggc ggtatcgatg gggtcggggt cggtgacttc
     1621 cagcacgagc gcaccgtcga tgatgataaa cgcgtggcca tcgacgctgc ccatctgaat
     1681 atggtattcc cttccgggat gaaaatcaaa gaggggattt gcggcgttga gcttgtaatc
     1741 gggcgacttt tcaaacccga ccttgccgtg ccaccagcct tccagcccgc caacgtaggc
     1801 tacgtcccgc gtgttggttt gctcgttcca gctgccgttc cacatggcgt tgatatcgtg
     1861 cgtgctgggc gacaccgtat tcgccgtgaa ctccagcaaa acatttccgt agaaatcttt
     1921 gcgcgagatg accatgccgg ggaagttgcc gcggttttcg ccgtagagcc agccgtcttc
     1981 cacgtgccac tgcccgctgc gaatgtcgaa atcctcgcgc agaacgtctt ccgtcaattc
     2041 cttgtgccag agcacggggc attgatcgag cagcagttcc ttgttcatca ggcgcatagc
     2101 ggttctccaa tccggggcag aattgaattc tggcgtgaaa aacgaacgat ctgcctgatt
     2161 ataatacaga ttggtacgtg cgaaaagggg cggataaaaa agaaacgccg aaaagaaggc
     2221 gtttcctgtg cgggcgtatg cgaaaagtaa gttacttcat gcgctgaacg ccgatcatcg
     2281 cttcgtagag cgccacgaac tggcggtgct tgcgctcgtt gcgttcgtcc gcttgaggag
     2341 caaacgtcgc ttccagccgg acggcggcat tcgccgcttc cgaaaagtct ttgtaaacgc
     2401 ccgcgctcgt tgcgccgatc atggcggcgc cgagacacgc cgcttccttt tctgccggaa
     2461 tagagacggg aatgccggtg atatccgcct gcagctgaca ccagagcgcg gacttggccc
     2521 cgccgccggt ggcgatgatg cgctc
//
LOCUS       DAOMGY010000201         2539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_45027_length_2539_cov_3.363929, whole genome shotgun sequence.
ACCESSION   DAOMGY010000201 DAOMGY010000000
VERSION     DAOMGY010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45027_length_2539_cov_3.363929"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..497
                     /gene="tadA"
                     /locus_tag="PLR57_03930"
     CDS             51..497
                     /gene="tadA"
                     /locus_tag="PLR57_03930"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HQQ40637.1"
                     /translation="MNEAFERFMHAALEEANLAASKGEVPVGAVLVKDGEIVARAHNN
                     VEQTHDATAHAELLCLRQGMELLGARLTDCTLFVTLEPCAMCAGACVNAKLGRLVFGA
                     FDESAGCCGTRMDLTDHCFLHTVETWGGVLETECKKLLSSFFKELR"
     gene            633..1568
                     /locus_tag="PLR57_03935"
     CDS             633..1568
                     /locus_tag="PLR57_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQQ40638.1"
                     /translation="MEFLRLLEEIRTPLFNAIFQFFTLFGEETLFMVFAMVFFWCVDK
                     KSGYFLLYVSFLGATINQFLKLVFCVPRPWVLDKNFTIVESARDAATGYSFPSGHTQS
                     AAGLFLGIARIRKEIWIRIVCVTIALLVAFSRMYLGVHTPADVLVSLGVALLLVFGAY
                     PLFQLAWKNGKWFILLLGLLLVTGVALLMYVEFVPLPANAILEFSADGAKTAYTMFGT
                     SLGLMFVLWIEQKYIKFSVKAVWWAQILKAAIGLGLVIAVRLLLKEPLLALCGGHNVA
                     HTIRYFLMVALGGAVWPMTFKFWGNLGNKKQAAKA"
     gene            complement(1632..>2539)
                     /locus_tag="PLR57_03940"
     CDS             complement(1632..>2539)
                     /locus_tag="PLR57_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQQ40639.1"
                     /translation="ISPKNKACGALFFLGSEMFYRQHLALTDARGSCNVVTVYNAFFE
                     NGTSYAVMERLEGVTLEAYLRLKKRRLTPEEAMFIAASMADALLVVHSLNSLHYDINA
                     KSIFLCTDGSVKLIDFGAAKAGLRARREVDDTEPWLDLGALGKTLYEAMSGQTLYGTQ
                     ISPSANIPAPFYDLILRMLHLDGARRFGSVFEYRHALACLEIGAACPAVTSEEAGMQL
                     PPLKKAESRRAEPQPQPQEAPPQKKTILDRALDTLQEAVETSPVEITPEVEQKRKHLT
                     RRVILFYVAIAVVTLVAALVIKSLT"
BASE COUNT          486 a          720 c          727 g          606 t
ORIGIN      
        1 ctgatgcggg tggtttacga cgggtttgat accgaggagg tgctttcccg gtgaacgaag
       61 cgtttgagcg gttcatgcac gcggcactgg aggaagcgaa tctcgccgca agcaagggcg
      121 aagtgcccgt aggcgcggtg ctggtcaaag acggcgaaat cgttgcccgc gcgcataaca
      181 atgtggaaca aacgcacgac gccaccgcac acgcggaact gctctgcctt cgtcaaggta
      241 tggaactgct cggcgcgcgc ttgacggact gcacgctgtt tgtaacgctg gaaccctgcg
      301 ccatgtgcgc gggcgcctgc gtcaacgcca agctcggcag actcgttttc ggtgcgttcg
      361 acgaatccgc gggctgctgc ggaaccagaa tggatttaac ggatcactgc tttctgcaca
      421 ccgttgaaac atggggtggc gttctggaaa cggagtgcaa aaaactgctt tcgtcgttct
      481 ttaaagagct tcgctgacgc gttctttatc tccatcgtca gtgtgtgatg agcggcattc
      541 gccgctttct ttttactgta aatccatgtt atactgaaca aaatcaagtc tgtattcttt
      601 gtcaaaaaac atatttggga ggggtattct gcatggagtt tttacgactg ctggaggaaa
      661 tccgcacgcc gctgttcaac gcgatcttcc agttttttac gctgttcggc gaggaaacac
      721 tctttatggt gttcgccatg gtgttttttt ggtgcgtcga taaaaagagc ggatattttc
      781 tgctctacgt gagctttctc ggcgcaacga tcaaccagtt cctcaagcta gtcttctgcg
      841 tcccccgccc ctgggtgctg gacaagaact ttaccattgt tgaaagcgcg cgggacgcag
      901 caaccggcta ctccttcccc agtgggcata cgcagagcgc ggcggggtta ttcctcggca
      961 ttgcgcggat acgaaaggaa atctggattc gcatcgtctg cgttactatt gcgctgctgg
     1021 tcgcgttttc gcggatgtat cttggcgtgc atacccccgc ggacgtgctt gtttcgcttg
     1081 gtgtggcgct cctgctggtg ttcggcgctt atccgctctt tcagctcgcg tggaaaaacg
     1141 gcaagtggtt catcctgctg ctcgggcttc tgctggtgac gggcgttgct ctgcttatgt
     1201 acgtggagtt cgtgccgctt cccgccaacg ccatactcga gttctccgcc gacggcgcaa
     1261 agaccgctta tacaatgttc ggcacgtcgc ttggactgat gtttgtgctc tggatcgagc
     1321 agaagtacat caagttcagc gtaaaggccg tctggtgggc gcagatcctc aaagccgcca
     1381 tcggcctcgg tcttgttatc gctgtgcgcc ttctgctcaa ggagccgctt cttgcgctct
     1441 gcggcggtca caacgtggcg cacaccattc gttatttcct catggtcgcg ctcggcggcg
     1501 cggtctggcc gatgacgttc aaattctggg gtaacctcgg caacaagaag caagccgcga
     1561 aagcttaaaa tacaaaacaa taaatcgccc cgtgcaatac ggggcgattt ttgtttgatt
     1621 tgttcttatt cttacgtcaa gctcttaatt acaagcgcgg caaccagcgt gaccaccgca
     1681 atggcaacgt aaaacaggat cacccggcgg gtcaggtgct tgcgcttctg ctccacttcc
     1741 ggcgtgattt ccaccggtga agtctcaacc gcttcctgca gggtatccag cgcgcggtcg
     1801 aggattgtct tcttttgcgg aggcgcttcc tgcggctggg gctggggttc tgcacgcctg
     1861 ctttccgcct tcttgagcgg cggcagctgc atgcccgctt cttcgctggt caccgcgggg
     1921 cacgccgcgc cgatttccag acacgccagc gcatgccggt attcaaacac agaaccaaac
     1981 cgccgcgcgc cgtccaggtg cagcatgcgc agaattaaat cataaaacgg cgcaggaata
     2041 ttcgcgctgg gcgagatctg ggtgccgtaa agcgtctgcc cgctcatcgc ctcgtacagc
     2101 gttttgccga gagcgcccaa atcgagccac ggttcggtat cgtccacttc ccgccgcgcg
     2161 cgcaggccag ctttcgccgc gccaaagtcg atcagcttga cgctgccgtc cgtgcagaga
     2221 aagatggact ttgcgttgat atcgtagtgc agcgagttga ggctgtgcac gaccagcagc
     2281 gcgtccgcca tggaagccgc gataaacatg gcttcctccg gcgtgagcct gcgctttttg
     2341 agtcgcaggt acgcttccag cgtaacgcct tcgagccgct ccatcaccgc gtaggaggtt
     2401 ccgttttcga aaaacgcgtt gtaaacggtg accacgttgc agcttccccg cgcgtccgtc
     2461 agcgccagat gctggcggta gaacatttcc gagcccagaa agaacagcgc gccgcaggcc
     2521 ttgttctttg gcgatatcg
//
LOCUS       DAOMGY010000202         2531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_45251_length_2531_cov_3.025040, whole genome shotgun sequence.
ACCESSION   DAOMGY010000202 DAOMGY010000000
VERSION     DAOMGY010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45251_length_2531_cov_3.025040"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="PLR57_03945"
     CDS             <1..1308
                     /locus_tag="PLR57_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQQ40640.1"
                     /translation="TAANNGKLVARLDEGTVVKILETNVFSEWYRIEAGTRTGYVNRM
                     YVNIDPSLPSYQLDCTGKVFNCAEFVNVRATPSMKAKKLGKANKDEIFKVTQAYASGA
                     WHQIDYNGKTGYVSSEYLLLTATVGDDLISGLEISGGTLSPRFSPTEYGYVLTANQSE
                     VIIKATANEGVKVSIGSTGIASAKYTINSGNSKTIRIYVGGKVKYSIYLVRDVLTVGT
                     WNIKRGNDNLVMQGWLIAAEKPDILGVQEVYVNSKEKTDNLLSVRTRDASYTSFAETV
                     AYPSGGQYGIGQISKFKPVEQEIIPLYSADKEVRCLQRVEYEIDGKVVSVYNTHFSFE
                     SASIRKKQFAEVLAAMDADTNQYKILTGDFNADETEFAAFSKNYHVVNTSATKFYDYS
                     YKRISMSQIDNIVVSNNITVLNARAIPNSYSDHYPLFAFLALK"
     gene            1620..2147
                     /locus_tag="PLR57_03950"
     CDS             1620..2147
                     /locus_tag="PLR57_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40641.1"
                     /translation="MLQHILEGRRADRRTDSLVYDVLARARKCPKCGHQQSWFPVQRL
                     FTFSANRYNRKHPMLSEPDIVFCGNSPQEEFDLLEKPCSITVVHHAEASEANEPGTYL
                     CINEMTKGKAYGSFYYNQDTRFGDNVITVRSLSGIVVLTYYFRAESGAGIKIYYEHFK
                     FEITAVFPPDSSEQG"
BASE COUNT          650 a          666 c          660 g          555 t
ORIGIN      
        1 accgccgcca acaacggaaa gctggttgct cggctggacg aaggcacggt cgtaaagatt
       61 cttgaaacga atgtgttttc cgagtggtac cgcatagaag cgggcacccg tactggctac
      121 gtcaaccgca tgtacgtcaa catcgatccc agcctgcctt cctatcagct tgactgcacg
      181 ggcaaggtgt tcaactgcgc ggagtttgtc aatgtccgcg caacgccctc catgaaggcc
      241 aaaaagctcg gcaaggcgaa taaggacgaa atcttcaaag tcacgcaggc atacgcttcc
      301 ggcgcgtggc atcaaattga ttacaatgga aaaacagggt atgtttcctc agaatacctg
      361 ctgttgaccg ctaccgtggg ggacgatctc atcagcgggc tggagatttc cggcggcacg
      421 ctctcgccgc ggttttcgcc caccgaatac ggctatgtgc tcaccgcaaa ccagagcgaa
      481 gtcatcatta aggccaccgc aaacgaaggc gtaaaggtaa gcatcggttc aacgggtata
      541 gcaagcgcaa agtacaccat caactccggc aacagtaaga ccatccgcat ctatgtcggc
      601 ggcaaggtca agtactccat ctatcttgtg cgggacgtgc tcacggtagg cacatggaac
      661 atcaagcgcg gcaacgacaa tctcgtgatg cagggctggc tgatcgcggc cgaaaagccg
      721 gatatcctcg gtgtgcagga agtctacgtc aactccaagg aaaaaaccga caacctgctc
      781 tccgttcgca cgcgggacgc ttcgtatact tcctttgccg aaacggttgc gtatccttcc
      841 ggcgggcagt acggcattgg ccagatttcc aaattcaagc cagtcgagca ggagattatc
      901 ccgctctaca gcgcggataa ggaagttcgc tgtctccagc gcgtggagta cgaaatagac
      961 ggcaaggtcg tctccgttta caacacgcat ttttcattcg agtccgcctc catccgcaaa
     1021 aagcagtttg ccgaagtgct tgcggctatg gacgcggata cgaaccagta caagattctg
     1081 accggggact tcaatgccga cgaaaccgag tttgcggcgt tcagcaagaa ctaccatgtg
     1141 gtcaacacct ccgcaacgaa gttctacgac tattcgtaca agcgaatctc catgtcccag
     1201 atcgacaaca tcgttgtaag caacaacatc accgtgctca acgcgcgcgc catcccgaat
     1261 tcgtactccg atcactaccc gctgtttgcg tttctagcgc ttaaataatg ctcgaagata
     1321 aaccacaaaa taaaccgcat tgtgtgccgg gtgcacacca tgcggttctt tttatttgct
     1381 agaatgaacg cataagcaag atataatttg tttggagcgt gcagaatatg attcgttacc
     1441 aatcgccgga acggtacgca aacaggcttt actatcggta tacctgcgaa caatgcgggt
     1501 ttcacagcca ttggatgatg cagtatttca gagatttgcc cagcagcgcg gaagtgggat
     1561 ttggccccgg ctcggagccg ggttctccgg tgccatatca ttttaagcgg ctcgatatta
     1621 tgcttcagca tatactcgaa ggcaggcgtg cggataggcg aaccgactct ctggtctacg
     1681 acgttttggc gcgtgcgcgc aaatgcccga aatgcggtca tcagcagtcc tggtttccgg
     1741 tgcagcggtt gtttacattc agcgcgaata gatacaacag gaaacatccc atgctgagcg
     1801 agccggatat cgtgttctgc ggaaattcgc cgcaggaaga attcgatctg ctcgaaaagc
     1861 cgtgttccat tacagttgta caccatgccg aagcttcgga agccaacgag ccgggaacgt
     1921 atctttgcat caacgaaatg acaaagggca aagcgtacgg ttcattttac tataaccaag
     1981 atacgcgctt cggcgacaac gtaataaccg ttcgaagctt atcgggaatc gtggtgttga
     2041 catactattt ccgggcggag agcggcgccg gcataaaaat atactatgaa catttcaaat
     2101 tcgaaatcac agcggttttt cccccggatt cgtccgaaca aggctgatca gcggcttgtg
     2161 aaatattatc tctgttgagc agttcaatca gattcacaac aagatgaatc cgtgtgatgg
     2221 agagctgccg aagccggaac tgctgagaaa gtatcagaat agacggcaat cgctccagcc
     2281 taaaacccgc acgaaactct aaccaaaagc tcatacgaaa atacaaagcg tcgccctgca
     2341 gggcggcgct tttgcgttac ttgacttcgt ttcgcttaat gcgcatacgt gccgttgaga
     2401 acggtagtgg cgagcacctt gccctcgatg gcgctggtca gctctttcag cgaaaagccg
     2461 tttgacagcg cgggaacttc cgaaagcgcg taggcggtgg tgacgtatgt gtgcgtaccc
     2521 gtgggcgggg t
//
LOCUS       DAOMGY010000203         2526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_45397_length_2526_cov_3.140024, whole genome shotgun sequence.
ACCESSION   DAOMGY010000203 DAOMGY010000000
VERSION     DAOMGY010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45397_length_2526_cov_3.140024"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..712
                     /gene="nrdR"
                     /locus_tag="PLR57_03955"
     CDS             257..712
                     /gene="nrdR"
                     /locus_tag="PLR57_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HQQ40642.1"
                     /translation="MKCRYCASIESKVIDSRPTEDGSAIRRRRECINCGKRFTTYEKI
                     EDIPVMVVKRDGRREPFDSEKIRNGIRKACEKRPIAADVQDKVVEDVTREVFNTLASE
                     VTTRDIGEIVMRKLKDVDEVAYVRFASVYREFKDTQTFMKELQRLLDEK"
     gene            898..1749
                     /locus_tag="PLR57_03960"
     CDS             898..1749
                     /locus_tag="PLR57_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HQQ40643.1"
                     /translation="MTQKQDFHTFYQNAERGAKLRFVNEIGSYTSPSLSALSGFDHGF
                     SARTGGVSEGFFSSLNLSFTRPEEPANVMQNYRLFCNAMGILFESMVMDNYAHETTVL
                     CVSSGDAGRGYLREPLPSCDGLVTNDPSITLITGHADCMAFFCIDPATKSIGLAHAGW
                     RGALGRIGANVIKQMTEHFGTNPADVLVSVGPSICSNCFEVGRNVSEAFEQEFGDIPC
                     IVSRAGEKAHVDLWMVAAKQFYDAGVQAGHFSLFDVCTMETPLLYSHRRDKGNTGGMA
                     AYLRILA"
     gene            1907..>2526
                     /locus_tag="PLR57_03965"
     CDS             1907..>2526
                     /locus_tag="PLR57_03965"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQQ40644.1"
                     /translation="MMKKKVVSILLVCALLLTMGFAGCAKQETLAAKAGDREITLQQL
                     ENSYSNSSAYASYSGYSLDTDEGIAAYVEYLLDNLIASNMKIYQAKLAGITLTDEEIA
                     AAKESAQSNYDDTYQSFIDQATQAGASNVDAYAKTLFTNALVQNHTTVNKLKASMLED
                     AENETLVAKHKTALLEGVALTDDELLQKYNDELAAQKTLFDSDASQ"
BASE COUNT          658 a          647 c          703 g          518 t
ORIGIN      
        1 atgaaaagcc cagtgtgaag cgtaaaaaga aggccgaggc cgctcggaag cgcaaatatt
       61 aatcgaaaaa acaacgatta caaagacgaa ccgcaaagcg gttcgtcttt tgtttgcctt
      121 tttcagcctg cattcgatga ctgctgacag caagcgacag gtcgtcggaa gccaataaag
      181 cagattctag tggtatttat actatatata tgatagtatg taacaaacga atgcaccata
      241 taaggagcgt cccattatga aatgcagata ttgcgccagc atcgaaagca aggtaatcga
      301 ttcgcggcca accgaggatg gcagcgccat ccgccgcagg cgcgaatgca tcaactgcgg
      361 caaacgcttt accacgtatg aaaaaataga agatatcccc gttatggtgg tcaagcgcga
      421 cggcagaagg gaaccgttcg attccgaaaa gattcgcaac ggcatccgca aggcctgcga
      481 aaagcgcccc atcgcggcgg acgtgcagga caaagtggtg gaggacgtaa caagggaagt
      541 gttcaacacc ctcgcttccg aagtgaccac gcgggatatc ggcgaaatcg tcatgcgtaa
      601 gctcaaggac gtggatgaag tcgcctatgt gcgctttgcc tccgtctacc gcgagttcaa
      661 ggacacgcag acgttcatga aagagttgca gcgtttgctg gacgaaaaat aagaggattg
      721 tttttcagag gaacttcagc gtttgctgga cgaaaagtaa gacatcagtt actcagaaga
      781 tctgcagcgt ttgctggacg aaaaataaga ggattgtttt tcagaggaac tgtagtgctg
      841 ctggacgaga aataagagca aaagattaaa ggaacagcgg cgctcgccgc cggaaagatg
      901 acgcagaaac aggatttcca tacattttac cagaatgcgg agcgcggagc aaagctacgc
      961 ttcgtaaacg aaataggcag ttacaccagc ccctcgctga gcgcgctgag cgggttcgac
     1021 catgggtttt cggctcgaac cggcggcgtg agcgagggct ttttttcgtc tctgaatttg
     1081 agctttaccc gccccgaaga accagcaaac gtcatgcaga actaccgcct cttctgcaac
     1141 gcaatgggaa ttctgtttga gtccatggtg atggataact acgcccatga aaccacggtg
     1201 ctctgtgttt cttccgggga tgcggggcgt gggtacctgc gcgaaccgct gccgagctgc
     1261 gacgggcttg tgaccaatga tccttccatc actctgatca ccgggcacgc ggactgcatg
     1321 gcgtttttct gcatcgatcc ggcgacaaag agcatcgggt tggcgcatgc aggctggcgc
     1381 ggcgcgctgg gcagaatcgg cgcgaacgtc ataaagcaaa tgacagagca cttcggcaca
     1441 aaccccgcgg acgtgctggt ctccgttggc ccctccatct gttcgaactg ctttgaagtc
     1501 ggcagaaacg tctcggaggc gtttgaacag gagttcggcg atattccctg cattgtctcg
     1561 cgcgcgggtg aaaaagcgca tgtcgatcta tggatggtcg ccgcaaaaca gttttacgac
     1621 gcgggcgttc aggccgggca tttttcactg ttcgacgttt gcacaatgga aacgccgctt
     1681 ctgtattccc accggcgaga caaggggaat accggcggca tggcggctta cctgagaatc
     1741 ctcgcctgac cgaatcggtt tcagcagacg ttactgccgg gaatgcccgt tttgcgcgca
     1801 tcgcttgatt gacaagcaac gcgccccaga gtataattca ctcgtttccg gctgttattt
     1861 cggctcgaga aatggatatt accaaagcaa gggagactag tttctcatga tgaagaaaaa
     1921 ggtcgtttca atccttctgg tctgcgcgct gctgctcacg atgggctttg cgggctgcgc
     1981 aaaacaggaa acgctcgcgg caaaggccgg cgaccgtgaa atcacgctcc agcagctgga
     2041 aaactcctac agcaacagct ctgcctacgc ttcctactcc ggctactcgc tggatacgga
     2101 cgaaggcatt gccgcttatg tcgaatacct gcttgacaat ctgattgcaa gcaacatgaa
     2161 gatctatcag gcgaaacttg cgggcattac gctgacggac gaagaaatcg ccgccgcgaa
     2221 ggaatctgca cagagcaatt acgacgatac gtatcagtcg ttcatcgacc aggcaacgca
     2281 ggccggtgcg agcaatgtag acgcgtatgc caagacgctc tttaccaacg cgctggtgca
     2341 gaaccacacg acggtgaata agctcaaggc ctccatgctg gaggacgcgg aaaacgaaac
     2401 gctcgttgcc aagcacaaga cggcgttgct tgaaggcgtc gcactgacgg atgatgagct
     2461 cctgcagaag tataacgacg agctcgccgc gcaaaagacg ctcttcgatt ccgacgcgtc
     2521 gcagta
//
LOCUS       DAOMGY010000204         2524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_45444_length_2524_cov_3.636695, whole genome shotgun sequence.
ACCESSION   DAOMGY010000204 DAOMGY010000000
VERSION     DAOMGY010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45444_length_2524_cov_3.636695"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLR57_03970"
     CDS             complement(<1..558)
                     /locus_tag="PLR57_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQQ40645.1"
                     /translation="MPKAPEQLRLENIIQRNMDDVMHQSMMPYAEHVILERALPRVED
                     GLKPVQRRILYTMLELSLTPDKPHRKSARIVGDCLGKYHPHGDSSVYDAMVRMAQPYN
                     MCVPLVDGHGNFGSVDGDSAAAMRYTEARMTPAAMQLLRDIEKDTVSYSFNFDDTLKE
                     PDLLPGRFPNLLVNGASGIAVGLATN"
     gene            complement(677..>2524)
                     /locus_tag="PLR57_03975"
     CDS             complement(677..>2524)
                     /locus_tag="PLR57_03975"
                     /inference="COORDINATES: protein motif:HMM:NF004189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HQQ40646.1"
                     /translation="HLLWEIIDNSIDEAANGYANEISVTLHKDNSVTVTDNGRGIPVQ
                     IHEKAGVSGVEVVFTQLHAGGKFGNDSYGYSGGLHGVGASVVNALSEWLKVEVCTDGR
                     LYGVEFRSEADETGKIVSGKTVAPLTELGRTRKQGTTITFMPDERVFETVEMNFDVIS
                     RRLRELAYLNRGTKMVLSDEHTGHQRKAEYQFNGGLSDFVCYLNGDKTPLFSTPIHFS
                     GASGDILVEVAIQYNDGYADNIFSYVNNIPTTEGGTHETGLKAALTKVLNDYCRKMGY
                     LKDKDAPLSGEDFREGMTAVLSLKMRTIQFEGQTKTKLGNTEARPAVETVVMEKLVPF
                     LEDLKNGEIAAIIAEKAVKAAKVREAARKAKATAREKNKLENAPLVGKLSSCTGRDYS
                     KNELFIVEGDSAGGSAKQGRDRRFQAILPLRGKPLNVEKKRLDQVLQNEEFRSIITAV
                     GTGIGEDFDISSLKYDKIIILSDADQDGAHIRAILLTFFYRYMKELITEGHVYIGLSP
                     LYKVQKDTKRIYCYDDDELRAATKSMGKGYTIQRYKGLGEMNPEQLWETTMNPDGRTM
                     LRVTIEDAADVEHIVVVLMGDKVQSRKEYIFEFADFNKSKDETFEKLKD"
BASE COUNT          434 a          708 c          767 g          615 t
ORIGIN      
        1 gtttgtggca agacctaccg cgatgccgct cgcgccgttg accaaaaggt tcgggaaacg
       61 ccccggcagg aggtcgggct ctttaagcgt atcgtcaaag ttaaaggagt agctgacggt
      121 gtccttttca atatcgcgca gcagctgcat ggcggcgggg gtcatgcgcg cttcagtata
      181 acgcatggcg gcggcgctgt ctccgtccac gctgccaaag ttgccgtgac cgtccacgag
      241 cggcacgcac atgttgtacg gctgcgccat gcgcaccatc gcgtcgtaaa cggagctgtc
      301 cccgtgcgga tggtatttgc ccagacagtc gcctacgata cgcgcgctct ttctatgcgg
      361 cttgtccggc gtcaggctga gctccagcat ggtatagagg atgcggcgct gcacgggctt
      421 taagccgtcc tcgacgcgcg gcagggcgcg ctccagaatg acgtgctcgg cgtaaggcat
      481 catggactga tgcatcacgt cgtccatgtt gcgctggatg atgttttcca gtctgagctg
      541 ttcgggtgct ttcggcatat cgaaaatcct cggttgtttg atttagtggc ttttctcgcg
      601 ggtttccggt tttggggaat ctgcttggct agtctttctt attggggctg tttcgcgggt
      661 ttccggctga ctgaagttaa tctttcagct tttcaaacgt ttcgtccttg gacttgttga
      721 agtccgcgaa ttcgaagatg tattccttgc gggactgtac tttgtcgccc atcaaaacga
      781 caacaatgtg ttccacgtcc gccgcgtcct caatggtgac gcggagcatc gtgcgcccgt
      841 ccgggttcat ggtggtttcc cagagctgtt cggggttcat ctcgccaagg cctttgtagc
      901 gctgaatcgt gtagcccttg cccatggact tggtggcggc gcggagctcg tcgtcgtcgt
      961 agcaatagat acgcttggta tccttctgca ctttgtagag cggggagagg ccgatataga
     1021 cgtgcccttc cgtaatgagc tccttcatat agcggtagaa aaacgtcaag aggatggcgc
     1081 ggatatgcgc gccgtcttga tccgcatccg agaggatgat gatcttgtcg tacttgagcg
     1141 acgagatgtc gaagtcctcg ccgatgccgg tgccgaccgc cgtgatgatg gagcggaact
     1201 cctcgttttg cagcacctga tcaagccgct ttttctcgac gttgagcggt tttccgcgca
     1261 gcggcaggat cgcctggaac cgccggtcgc gcccctgctt ggcggagccg cccgcgctgt
     1321 cgccctccac gatgaagagc tcgtttttgc tgtagtcgcg gcccgtgcag ctggagagct
     1381 tgccaacgag cggcgcgttt tcgagcttgt tcttttcgcg tgcggtggcc tttgctttcc
     1441 gcgcggcctc gcgcactttg gcggctttga ccgctttttc ggcgatgatg gcggcgattt
     1501 cgccgttctt taaatcttcc aaaaacggca cgagcttttc catgacgacg gtttccaccg
     1561 cggggcgcgc ttccgtattg ccgagcttgg tcttggtctg cccttcgaac tggatggtgc
     1621 gcatcttgag cgacagcacg gcggtcattc cctcgcggaa gtcctcgccg gaaagcggcg
     1681 cgtccttatc cttaaggtaa cccatcttgc ggcagtagtc gttgagcacc ttggtgagag
     1741 ccgctttcaa acctgtttcg tgcgtgccgc cctcggtggt cggtatgttg ttgacgtagg
     1801 aaaagatgtt gtcggcgtag ccgtcgttat actgaatggc gacctctaca aggatatcgc
     1861 cggaagcgcc ggaaaaatga atcggcgtac tgaacagggg cgttttatcg ccgttgaggt
     1921 agcacacaaa gtcgctaagg ccgccgttga actggtattc cgccttgcgc tggtggccgg
     1981 tatgctcgtc ggaaagcacc atcttcgtgc cgcggttaag gtacgccagc tcgcgcagac
     2041 gccgggagat gacgtcaaaa ttcatctcca cggtttcaaa cacgcgctcg tccggcatga
     2101 acgtaatcgt cgtgccctgc ttgcgcgttc gcccgagctc ggtcagcggc gctacggttt
     2161 tgccggagac gatcttgccc gtttcgtccg cttcactgcg aaactctacg ccgtagagcc
     2221 gcccgtccgt gcagacctcg accttaagcc attcggatag cgcgttgacc accgaagcgc
     2281 caacgccgtg gagcccgccg gaatagccgt aggaatcgtt gccgaacttg ccgcccgcgt
     2341 ggagctgggt aaacacgacc tccacaccgg aaacgcccgc cttttcgtga atctgaacgg
     2401 gaatgccgcg gccgttgtcg gtaacggtca cggagttatc cttgtgcagc gtgacggaga
     2461 tttcgttggc gtagccgttg gcggcttcgt cgatggagtt gtcgatgatt tcccagagga
     2521 gatg
//
LOCUS       DAOMGY010000205         2499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_46107_length_2499_cov_3.198445, whole genome shotgun sequence.
ACCESSION   DAOMGY010000205 DAOMGY010000000
VERSION     DAOMGY010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46107_length_2499_cov_3.198445"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..767
                     /locus_tag="PLR57_03980"
     CDS             351..767
                     /locus_tag="PLR57_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40647.1"
                     /translation="MTRHFLFWRFLNGRLGLLLLGVLIAVGIVLLIVLLTSRKYPRGN
                     VKTAPGAQPAASAAGTPASAVTGDPHGKILDLLRVASEEKQVSAADYEERRMVLDGGK
                     YDNYRRAELVELKEKYARFELSTQEYIEARAKLLNL"
     gene            complement(833..1801)
                     /locus_tag="PLR57_03985"
     CDS             complement(833..1801)
                     /locus_tag="PLR57_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40648.1"
                     /translation="MIRRGGVATHPSILQYLSDQACGEMPLWLFARVCGEEANAEEMR
                     ASRHYLPLANEQREDGGYGRFHSMDSLDKTSRLFRTTEQAVQRMRDLMLPHDDPVVLR
                     VQSALLGYADGTLAYSDRIERHPDFDIALQSMAAANAAALGSTDARVEQRKAVCAQYV
                     AAAAEAIASGGDYESAWRASALERHDLALKPETIHVLWLLWQNPYLEEEAEHQFLRYL
                     WGRSIYYTPGIPAREKRFADSAQFARWLFVLEQFSGARTFPRLMREHQTNEFLEQEAL
                     RLAGGVAEPYPNGELLGRYQEGKRTKPGLIWDTVLRIARLLQAAER"
     gene            complement(1929..>2499)
                     /locus_tag="PLR57_03990"
     CDS             complement(1929..>2499)
                     /locus_tag="PLR57_03990"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HQQ40649.1"
                     /translation="FAYGAFAGMMAGLFEETGRYSGFAVLLRKKRGKDINGLMYGAGH
                     GGIEAVLVMSLAMVSNLVFAFSVNAGTADPSMASIAAQLAGIAPVTFLVSIVERIGAV
                     ALHISLSVLVWFAVKNRKRFWLFPLAIVLHALVDAVAVIMSRFVTNIWLIEAAVYLLA
                     GVCAVLAVIVYKRNSKPAEMAADPAVDIA"
BASE COUNT          575 a          759 c          678 g          487 t
ORIGIN      
        1 tcgagtgcaa gaaatgcggc aacgtctacg agtacctcaa aaacaaccgc gtctgcccca
       61 agtgcggcgc atgggaatgc gtcatggtga cgggtcagga gttcaacatc aaagagattg
      121 tggtgtatga ggaggacgag ccggaagagc cgtaaccccg cgtcatctgt ttttgcccgc
      181 ttcggcgggc ttttttcttg cagtgcctga ggaatcggag caaatcaata aagtttatca
      241 aaaaaacata actccaacac ttttttaaca caaatgagat ttatgatgaa tttgcaataa
      301 taaaagtgtt tccgttatgg aagcacgaga aatgggagat tgctatgctg atgacacgtc
      361 attttctgtt ctggcggttc ctcaacggaa ggctgggtct gctcttgttg ggcgtactga
      421 tcgccgttgg aatcgtgctg ctgatcgtat tactgaccag ccgtaaatat ccccgcggaa
      481 acgtgaagac cgcgcccggc gcgcaacctg ccgccagtgc cgcgggcacg cccgcaagcg
      541 ccgtgaccgg cgacccgcac ggaaagattc tcgacctgct gcgcgttgcc agcgaggaaa
      601 agcaagtaag cgccgcggac tatgaagagc gccgtatggt gctggacggc ggaaagtacg
      661 ataactaccg ccgcgccgaa ctcgtcgagc ttaaggaaaa atacgcccgc ttcgaactct
      721 ccacgcagga atacatcgaa gcgcgcgcca aactgctgaa cctgtaacac cgaaacataa
      781 aacaaaaaga cccgcaaggg tctttttgtt tgtctttata aactattgcg cgttaccgct
      841 ccgccgcctg aagcaaacgc gctatccgca aaacggtatc ccagatcaga ccgggctttg
      901 ttcgcttgcc ttcctgatag cgcccgagca gctccccgtt cgggtaaggt tcggcaacgc
      961 cgccagcgag cctgagcgct tcctgctcta aaaattcatt cgtttgatgc tcccgcatca
     1021 gccgggggaa cgttctcgcg ccggaaaact gctccagaac gaacagccag cgcgcaaact
     1081 gcgccgaatc cgcaaagcgt ttttcccgcg ccggaatgcc gggcgtgtag tagatgctcc
     1141 tgccccaaag ataccttaaa aactgatgtt ccgcttcctc ttcgagatac ggattctgcc
     1201 agagcagcca gagcacgtga atcgtctccg gcttaagcgc aaggtcgtgc cgctccagcg
     1261 cggacgcgcg ccaggccgat tcgtagtctc cgccggaagc gatcgcctcc gcggcagcag
     1321 ccacatattg cgcgcagacc gccttccgct gctccacgcg tgcatcggtc gaaccaagcg
     1381 cggccgcgtt tgccgcagcc atcgattgca gcgcgatatc gaaatccggg tgacgctcga
     1441 tgcggtcgct gtaggcaagc gtgccgtccg cgtagccaag cagcgcgctt tgtacgcgca
     1501 gcaccaccgg atcgtcatga ggcagcatga gatcccgcat cctctgaacg gcctgttcgg
     1561 tggtgcggaa caggcgcgag gttttgtcca gcgagtccat ggaatggaac cgcccgtagc
     1621 cgccgtcctc gcgctgttcg ttcgccagcg gcaagtaatg gcgcgacgcg cgcatttcct
     1681 ccgcgttcgc ctcctctccg caaacccgcg caaaaagcca gagcggcatt tcgccgcaag
     1741 cctggtcgga cagatactgc agaatggacg gatgcgtagc gacacctccc cgccgaatca
     1801 taacggtatt tacctgtatt gtatctcgaa tttcgctgtc ccgccagata aacgcgctct
     1861 ttcagcgccg caaacgcgaa cacaaacaaa aaagaccctt tcgggtcttt ttgtttgctt
     1921 caattttgtt acgcaatgtc cacagcggga tccgccgcca tttccgcggg tttggagttg
     1981 cgcttgtaaa cgatgaccgc cagcacggcg cacacgcccg cgagcaggta caccgccgct
     2041 tcgatcagcc agatgttggt aacgaagcgg gacatgataa cggcaacggc gtccaccagc
     2101 gcgtgcagca cgattgcaag cgggaagagc cagaagcgct ttctgttttt cacagcgaac
     2161 cataccagca cggagagcga aatatgcagc gctaccgcgc cgatgcgctc cacgatgctc
     2221 accaggaacg tcaccggggc aattcccgcc agctgcgccg ctatggacgc catggacgga
     2281 tccgccgttc cggcgttgac cgagaacgcg aacacgaggt tcgaaaccat ggcaaggctc
     2341 attacaagaa cagcctcaat gccgccgtga cccgcgccgt acatcaggcc attgatatcc
     2401 ttgccgcgct ttttacgcag cagtacggca aagccggagt agcgtcccgt ttcctcaaac
     2461 agccccgcca tcattccggc aaacgccccg taagcaaag
//
LOCUS       DAOMGY010000206         2494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_46251_length_2494_cov_3.164002, whole genome shotgun sequence.
ACCESSION   DAOMGY010000206 DAOMGY010000000
VERSION     DAOMGY010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46251_length_2494_cov_3.164002"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PLR57_03995"
     CDS             complement(<1..895)
                     /locus_tag="PLR57_03995"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ40650.1"
                     /translation="MKTAKTGKVIPFSQTASFYRKRGEKELEKNDLIAAIHKYREAYE
                     RAPEDPEISIALAEILSQMQRFEESNRILLQLMASDQELPPEVHFGLACNYFGLQDYD
                     CAADSLEDYLDEEPDGPFAADAEDFLDLIDDDDAMYETTGLKTDDDYEDNAVCRFARS
                     LLASGDVGFAVEELEHRIGQKPDSVKVREQLSIAYFCDNRRADAERVANQLLAENPND
                     VLAICTLALGDLDANNQPAALEKLERLSQIKTSEPEELHSIAVLQMDLQRYEEAEKTL
                     SQLIVQMPYDENVLHKSGYVKF"
     gene            complement(882..1808)
                     /gene="hslO"
                     /locus_tag="PLR57_04000"
     CDS             complement(882..1808)
                     /gene="hslO"
                     /locus_tag="PLR57_04000"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HQQ40651.1"
                     /translation="MKKKQTGKQDRLIQCVAAEGMFRVTFIEGTRLVREARQIHGLSR
                     VATAALGRQLMMTAIMAADLKHEDDKVSTILNGDGPAGSMICTGNAALEVKGSVPNGI
                     SELPPTPEGKLDVGGFVGHTGKLTVVSDMGLKEPYVGVSNLVSGEIAIDFAQYYAASL
                     QRPALIYLGVRLNAESGEVLSAGGVFAEPMPGCPEELLDRLQQKSAEIVTLSKQLDAG
                     ETAEAFLERVFGEFGLTIASEREPVYRCDCSRERIERALISTGVQELRSMMEEDHGAG
                     VTCRFCDKIYTFTEEDLRELVNAASGNEDEDS"
     gene            complement(1847..>2494)
                     /locus_tag="PLR57_04005"
     CDS             complement(1847..>2494)
                     /locus_tag="PLR57_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQQ40652.1"
                     /translation="VHPGDSILDCACGTGELSIRLSKLGFLVTGIDRSEEMLEIAQKK
                     ARLAGQKIPFVCQDMRALSLHKPVSAVVCACDGVNYLLTKKDVGEFFNSAFAALKPNG
                     ALLFDVSSAYKLECVLGGQTYGEDTSECAYLWQNSFDPESRLIEMNLSFFVPDGAGKY
                     TRFTERHVQRAHTVEELQSALVKSGFNDCRVYEAFTRSEPNAASERIQFSAGKQL"
BASE COUNT          487 a          756 c          650 g          601 t
ORIGIN      
        1 tgaacttgac gtagccgctc ttatgcagaa cgttttcatc gtacggcatc tgcacaatca
       61 gttgactcag cgttttttcc gcttcctcat accgctgcag gtccatctgc aggacggcta
      121 tgctgtgtag ctcctccggt tcgctcgtct taatctgcga aagacgctcc agtttctcaa
      181 gcgcggcagg ctggttgttt gcgtcaagat cacccaaagc cagcgtgcag atggccagca
      241 cgtcgttcgg attttccgca agaagctggt tggcaacgcg ttcggcatcg gcgcggcggt
      301 tgtcgcaaaa atatgcgatg gaaagctgtt cgcgcacctt gacggagtcc ggcttctggc
      361 cgatgcggtg ctccagctcc tccacggcaa agcctacgtc cccgctggca agcagagagc
      421 gcgcaaaacg gcagacagcg ttgtcctcgt aatcgtcgtc cgttttcagg ccggtcgttt
      481 catacatcgc gtcgtcgtcg tcgataaggt cgaggaaatc ttccgcgtcc gcggcaaacg
      541 ggccgtccgg ctcttcgtcc agatagtctt ccagactgtc cgccgcgcag tcgtagtcct
      601 gcaacccgaa ataattacag gcaagtccaa aatggacttc cggcgggagt tcctgatcgc
      661 tcgccatcag ctgcagaaga atgcggttgc tctcctcaaa acgttgcatc tgactgagga
      721 tttccgccag cgcaatggag atttccgggt cttccggcgc gcgttcatat gcttcgcggt
      781 atttatgaat cgccgcgatt aaatcgtttt tttccagttc cttttcaccg cgtttccgat
      841 aaaacgacgc ggtctggctg aacggtatta cttttcctgt tttagctgtc ttcatcttca
      901 ttcccggaag cggcgttgac cagctccctt aagtcttctt cggtaaaggt ataaatcttg
      961 tcgcagaacc ggcaggtaac gcccgcgccg tgatcttctt ccatcatgct gcgcagttcc
     1021 tgcactccgg ttgaaatcag cgcgcgctca atccgttccc gcgagcagtc gcagcgataa
     1081 acgggttccc gttcggaagc gatagtaagc ccgaactccc cgaacacgcg ctccagaaac
     1141 gcttccgccg tttcgcccgc gtccagctgt ttggagagcg taacgatttc cgcgctcttt
     1201 tgctgcaagc ggtcgagcag ttcctccggg cagcccggca tcggttcggc gaacacgccg
     1261 cccgcgctga ggacttcccc gctttcggca ttcaatcgaa cgccgaggta aatcaaggcc
     1321 ggtctttgaa ggctcgcagc atagtactgc gcaaaatcga tcgcgatttc accggaaacg
     1381 agattgctga ctcccacata cggttcttta agccccatat cgctgacgac cgtgagcttg
     1441 ccggtatggc cgacgaaccc gcctacgtcc agcttgcctt cgggagtcgg cggcagttcg
     1501 gaaattccgt tcggaaccga acctttcact tccagcgcgg cgttgccggt gcagatcatg
     1561 ctgccggcag ggccgtctcc atttaatatg gtagaaacct tgtcgtcttc gtgctttaag
     1621 tccgccgcca tgatggccgt catcatgagc tgtctgccca gcgccgccgt tgcaacgcgc
     1681 gacagcccgt gaatctgccg cgcctcgcgg acaagtcgcg tgccttctat aaacgtaacg
     1741 cggaacattc cttccgccgc cacgcactga atcagtcgat cctgttttcc tgtttgtttc
     1801 ttcttcatcc tgctccgtca tccaatgtat cgtattaatt ttcttattat aactgcttac
     1861 ccgccgaaaa ctgaatccgc tcgctcgcgg cgttcggttc gctgcgcgta aacgcttcgt
     1921 agacgcggca atcgttgaat ccgcttttta caagagcgct ttgcagttct tccaccgtgt
     1981 gcgcccgctg aacatgccgc tcggtaaagc gcgtatactt gcccgcgccg tcaggaacga
     2041 agaatgacag gttcatctcg atcaggcggc tctcggggtc gaacgaattc tgccaaaggt
     2101 atgcgcattc gctcgtgtcc tcgccgtagg tctgcccgcc caggacgcat tcgagcttat
     2161 aggccgatga aacgtcgaag agcagcgcgc catttggctt gagcgcggca aacgcgctgt
     2221 tgaaaaactc gccaacatcc ttctttgtca gcaggtagtt cacgccgtcg catgcacaga
     2281 cgaccgcgct caccggtttg tgcagtgaaa gcgcgcgcat atcctgacag acgaacggaa
     2341 ttttctgtcc cgcaagccgc gcttttttct gggcgatttc aagcatctct tctgagcggt
     2401 caatccccgt taccaaaaag ccgagcttgc tgagccgtat ggagagttcg cccgttccgc
     2461 aggcgcagtc caaaatggaa tctccggggt gaac
//
LOCUS       DAOMGY010000207         2492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_46316_length_2492_cov_2.478457, whole genome shotgun sequence.
ACCESSION   DAOMGY010000207 DAOMGY010000000
VERSION     DAOMGY010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46316_length_2492_cov_2.478457"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..706
                     /locus_tag="PLR57_04010"
     CDS             314..706
                     /locus_tag="PLR57_04010"
                     /inference="COORDINATES: protein motif:HMM:NF022378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2680 domain-containing protein"
                     /protein_id="HQQ40653.1"
                     /translation="MRKALLIIAIVILVLTPIAAYAIMAYSPARSNVGYTYSRQELTQ
                     QQEADLQESYQQMIDLRKETINKMVQDGLLTEEQGQQALEQLDEMADYHAENGYAFGY
                     GMMGGCRIGFDADDDFYGRGRMRGYNWN"
     gene            727..912
                     /locus_tag="PLR57_04015"
     CDS             727..912
                     /locus_tag="PLR57_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HQQ40654.1"
                     /translation="MMLLWIIPVGIVVYLLFAKSGKPLGTWVKSGETPEDVLAKRFVN
                     GEITEEEYKKMQKIIHE"
     gene            927..1175
                     /locus_tag="PLR57_04020"
     CDS             927..1175
                     /locus_tag="PLR57_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010025309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40655.1"
                     /translation="MMFTRGFGSYGMLCSGFGAAYPGLWLGLAALFVAALVVVIVLLS
                     KKSRKTTNDGAADALKLRYIKGELTEEEYLKMKDVLGK"
     gene            1210..1896
                     /locus_tag="PLR57_04025"
     CDS             1210..1896
                     /locus_tag="PLR57_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQQ40656.1"
                     /translation="MRYQILVVEDQPEIRTIVEKYLLKEGYAVLSAGDGLEALALFNS
                     STVHLVLLDVMMPGIDGFEVLNEIRTVSEVPVIMLTARQNEVDRVRGFRGGVDDYVVK
                     PFSAKELMLRVQRLLLRVYHEADEIVYRCGDLSLHAGSMKLYRGEAEIPVTTAEFQLL
                     SAFFRNQGRVMSREHLIEQAYGTDYDGYDRNIDSYIKKIRQKIEADPRHPRLLLTKYG
                     AGYQFGGSKI"
     gene            1893..>2492
                     /locus_tag="PLR57_04030"
     CDS             1893..>2492
                     /locus_tag="PLR57_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQQ40657.1"
                     /translation="MTIRKQWMLALVVSAALSVIVNSLVLNIRINRYFVDYSAENYNT
                     HVLQVVQFSTDALSADEYTTQQLEMQLTSHLSDPITRIRLYRADGELLSDVSVSRTMM
                     GMMRSGTAARMMGSSAEEIDSIDIQKDGVLLGKLMITRYSSIGSSIATRQFAFSLITN
                     SLLSFGIVFALTLILGYFLSKRMSKDLIQTAEQAVTIDLG"
BASE COUNT          595 a          652 c          704 g          541 t
ORIGIN      
        1 tgcccgcgca ctggccacag aaccatccct tttgctgttg gacgagcctg ccgcctacga
       61 aaagatggcg catgcctgca acccctatgg cgacgggaac gcttgcgcgc gcattgcgga
      121 tattctggag ggcaagccgt tttcgccctg ggcttgacgg aataattgaa aagaagcgat
      181 tcttctaccg acaacaatgg atcacccgga ccgggtggtc tttttttcgc gcctttttgc
      241 gcaatcataa ttcaacatta atctaacctt atttaatagt acagaatcca ttcacgacaa
      301 ggaggtgaac ctcgtgagaa aagcactact catcatcgca attgttattc ttgtgctcac
      361 tcccattgcc gcctacgcga tcatggcgta ttcgccggct cgcagcaatg tcggctatac
      421 ctattcccgg caggagctga cgcagcagca ggaggcggat ttgcaggagt cctaccagca
      481 gatgatcgac ctgagaaaag agacgatcaa caagatggta caggacggac tgctcaccga
      541 agaacaagga cagcaggcac tggagcagtt ggacgaaatg gcggactatc atgcagagaa
      601 cggatacgcg ttcgggtatg gcatgatggg cggatgccgc atcggttttg acgcggacga
      661 cgatttctac ggccgcggca gaatgcgcgg ttataactgg aactgacaag gaaggcggaa
      721 aataaaatga tgttactttg gattatcccc gtcggcatcg tcgtttactt gctgtttgca
      781 aaatccggca agccgctggg cacttgggtg aagtccggtg aaacccccga agacgttctt
      841 gcaaagcgtt ttgtcaacgg ggagatcacc gaagaagaat acaaaaaaat gcagaaaatc
      901 atccacgaat aaggaggtat gatacaatga tgtttacaag aggattcggt agttacggta
      961 tgttgtgcag cgggtttggc gccgcgtatc ccggcctttg gctggggctg gccgctctgt
     1021 ttgttgcggc gctggtcgtg gtcatcgtgc ttttatccaa gaagtcccgc aagactacga
     1081 acgacggcgc ggcggacgcg ctcaagcttc gctacatcaa gggcgagctg acggaagaag
     1141 agtacctgaa aatgaaggac gtcctcggga agtaattccc gaaaggctgc aatgctttga
     1201 ggtggcacgg tgcgatatca gatattggtt gtggaagatc agccggaaat caggaccatc
     1261 gttgaaaagt atcttttaaa agaaggatac gcggttttat ccgcaggcga cggactggaa
     1321 gcgctggcgc ttttcaacag cagcaccgtg cacctcgttt tgctggacgt gatgatgccg
     1381 ggcatcgacg gatttgaagt gctgaacgag attcgcaccg tatccgaggt gcccgttatc
     1441 atgctcaccg cgcggcagaa cgaagtggac cgcgtgcgcg gcttccgcgg cggcgttgac
     1501 gactatgttg taaaaccctt cagcgcaaaa gagctgatgc tgcgcgtgca gcggctcctg
     1561 ctgcgcgtgt accacgaggc ggacgaaatc gtctaccggt gcggcgacct cagcctgcat
     1621 gcaggcagca tgaagcttta tcgcggcgaa gcggagatcc ccgtcacgac ggcggaattt
     1681 cagctcctgt ccgccttttt tcgcaatcag gggcgcgtca tgtcccgcga acatctcatt
     1741 gagcaggcgt acggaaccga ttatgacggc tacgaccgca acatcgacag ttatattaag
     1801 aaaatccggc agaaaattga ggcggatccg cggcatccgc ggctgctgct gacaaaatac
     1861 ggcgccggat atcagtttgg aggaagcaag atatgacaat ccgcaagcaa tggatgctcg
     1921 cgctggtcgt ttcggccgcg ctgtcggtca ttgtcaactc cctcgtactg aacatccgca
     1981 tcaaccggta ctttgtcgat tattccgccg aaaactacaa tacgcatgta ctgcaggtcg
     2041 ttcagttctc caccgacgcg ttgtctgcgg acgagtacac cactcagcag ctggagatgc
     2101 agcttacgtc gcacttgagc gatcccatca cgcggattcg cctctaccgc gcagacggcg
     2161 agctcctttc ggacgtcagc gtcagccgca ccatgatggg catgatgcgc tctggaacag
     2221 ccgcgcgcat gatgggttcg tccgcagagg agatcgactc catagatatt cagaaggacg
     2281 gcgtactgct cggcaaactg atgattacgc gctacagctc catcggcagt tccattgcga
     2341 cgcggcaatt cgcgttttcg ctgattacaa acagcctgct gtcctttggc atcgtgtttg
     2401 cgctgacact cattctgggt tactttctca gcaagcgcat gagcaaagac ctgattcaaa
     2461 ccgcggagca ggccgtcaca atagacctcg gg
//
LOCUS       DAOMGY010000208         2491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_46346_length_2491_cov_2.959360, whole genome shotgun sequence.
ACCESSION   DAOMGY010000208 DAOMGY010000000
VERSION     DAOMGY010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46346_length_2491_cov_2.959360"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLR57_04035"
     CDS             complement(<1..270)
                     /locus_tag="PLR57_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HQQ40658.1"
                     /translation="MEGDREAAGISTRAARAAGLVPADDPGRAALLWACDPSSPLGQP
                     QPGLVELVAGLRPAQSLVLDLAYEPLRLQGELALDEDALDRVRRLH"
     gene            complement(289..483)
                     /locus_tag="PLR57_04040"
     CDS             complement(289..483)
                     /locus_tag="PLR57_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40659.1"
                     /translation="MSASFTEGISSFFSSYGILIVLMIAMFAIMIIPQRRRDKKVKDM
                     LSSLMLAADFDPFGYGGAPN"
     gene            complement(655..1017)
                     /gene="nifU"
                     /locus_tag="PLR57_04045"
                     /pseudo
     CDS             complement(655..1017)
                     /gene="nifU"
                     /locus_tag="PLR57_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068444.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
     gene            complement(1086..2255)
                     /gene="nifS"
                     /locus_tag="PLR57_04050"
     CDS             complement(1086..2255)
                     /gene="nifS"
                     /locus_tag="PLR57_04050"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HQQ40660.1"
                     /translation="MQRVYLDNAATTMVRQEVLDAMIPAFTTYYGNPSSLHEFARDAG
                     KLVDEARAKVANAIGAKPDEIVFTGGGSESDNMVLRGVAAANKKKGKHIITSAVEHHA
                     VLYTLQAMEREGVAELTILPVDEYGLVTPEQVKDAIRDDTVLVSIMFANNEVGTIMPV
                     AEIGKICREKGVLFHTDAVQAVGHVPINVSEMNIDMLSMSAHKLHGPKGVGALYIKKG
                     VRVPALIIGGGQEKKRRAGTENVPGIVGMGRALELAVEHMEANALRVGALRDKLMKAI
                     PAAIPEVKLNGHPTKRLPNNVNFSIRYIEGESILLMLDINGIAASSGSACTSGSLDPS
                     HVLLAMGLPHEIAHGSLRLTLSEFTTEQEIDYVLDLLPQIVQRLRDMSPLYHKAK"
BASE COUNT          476 a          734 c          675 g          606 t
ORIGIN      
        1 atgcaggcgc cggacgcggt ccagcgcgtc ttcatccagt gccagttcgc cctgcaggcg
       61 cagcggctcg taggccaggt cgagcaccag cgactgcgcc ggccgcaagc cggccaccag
      121 ctccaccagg ccaggctgcg gctggccaag cgggctggac ggatcgcagg cccacagcag
      181 cgcggcgcgg ccggggtcgt cggcaggcac caggccggcc gcacgcgcgg cgcgcgttga
      241 aatgcccgcg gcttcgcggt caccttccac gtcccgtgca tcgctgcctc agttcggcgc
      301 gccgccgtaa ccgaagggat cgaaatccgc ggccagcatc agcgaggaaa gcatgtcctt
      361 aactttcttg tcgcggcggc gctgcgggat gatcatgatg gcaaacatgg cgatcatcag
      421 aacgatgagg atgccgtagc tgctgaagaa gctcgaaata ccttcggtga aacttgcgct
      481 catatttaaa aaccaattcc cttcctataa tagaaactag tgtatatgat tccccgaaca
      541 gcgtcaagta tcacaatgtg ttaaaataga aacaaacaga ggcaaaatgt tgttttcgcc
      601 tctgaaatgt atgtgtcttc gaaagcgtca agctttgccc gcctgctccg ctttcttctt
      661 catatagtct tcaattgctg cttttaccgc ttcttcggca agcaccgaac agtgaatttt
      721 ctgcgggggc aaaccctcca gcgcttccac aacggcggcg tttgtcagtt ccagcgcctc
      781 gctgacgctc tttcctttaa tcatttcggt cgccatggag ctggttgcaa cggcggcgcc
      841 gcagccgaaa gttttgaact ttacgtcgcg tatgatctcg ttttcgtcga tatccagaaa
      901 gatctgcatg atatcgccgc acttggcgtt gccgaccgtg ccgacgccgc tggcgctttc
      961 aatttccccg acgttgcgcg gatttttgaa gtgatccatc actttttccg tatacataat
     1021 ctatatctcc tttattcttc cggcggggtt tcgcgccgtc ggcaaatttc gtactaagta
     1081 ccggtttact tcgccttgtg gtacagcggg gacatatccc tcagcctctg cacgatctgc
     1141 ggcagcaggt cgagtacata gtcgatttcc tgttcggtgg tgaactccga aagcgtgagc
     1201 cggagcgaac catgcgcaat ttcgtgcggc agacccatgg cgagcagcac gtgcgaagga
     1261 tcgagcgagc cgcttgtgca ggccgaaccg ctggaagccg cgatgccgtt gatatccagc
     1321 atcagcagaa tcgattcgcc ttcgatgtag cgaatgctga agttaacgtt gttcggcagg
     1381 cgctttgtcg ggtgtccgtt gagtttgact tccggaatcg ccgcaggaat ggccttcatg
     1441 agtttgtcgc gcagcgcgcc gacgcggaga gcgtttgctt ccatatgctc cacggcgagt
     1501 tccagcgctc gtcccatgcc gacgatgccg ggtacgtttt cggttcccgc acggcgcttc
     1561 ttttcctgtc cgccaccgat gatcagcgca ggtacccgca cgcccttttt gatgtacagc
     1621 gcgccgacgc ccttgggtcc gtgtaactta tgcgcggaca tgctgagcat atcgatgttc
     1681 atttcggaaa cgttgatcgg cacatgccca accgcctgca ccgcgtccgt atgaaacagc
     1741 acgccttttt cgcggcagat tttgccgatt tccgcaaccg gcatgatggt gcctacttcg
     1801 ttgttggcaa acataatgga aacgagcacc gtatcgtccc gaattgcgtc cttcacctgt
     1861 tcgggcgtaa cgagtccgta ttcatccacg ggcaggatgg tcagctcggc aacgccttcg
     1921 cgctccatcg cctgcagcgt gtacagcacc gcgtgatgct ccaccgcgct ggtgatgatg
     1981 tgctttccct tcttcttgtt ggcggcagca acgccgcgca gaaccatgtt atcgctctcg
     2041 gaaccgccgc ccgtgaatac gatttcgtcc ggctttgcgc ctatggcatt ggcaaccttg
     2101 gcgcgcgctt catcgacgag tttgcccgcg tcccgcgcga attcgtgcag gctcgaggga
     2161 ttgccgtaat atgtggtaaa cgcgggaatc atcgcgtcta aaacttcctg ccggaccatc
     2221 gtggttgccg cattatccag gtacactctt tgcatacgct gctgacctct cttcttctct
     2281 gtctctatgt gataaacctt gcggttcgtc agtcttgata aatacttcgg ttattcgcgg
     2341 tttcgcgctg gaaccggcga atcgccctgc ccgcaaaagg agcaggcttt ttcgtatcgt
     2401 ttcttctgct ccaaatagtc gtccgcgagc tcctgaatcg tggtcttttc caatatgtcg
     2461 ttgattctgg actgaagctt gaggaataaa g
//
LOCUS       DAOMGY010000209         2489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_46415_length_2489_cov_2.837716, whole genome shotgun sequence.
ACCESSION   DAOMGY010000209 DAOMGY010000000
VERSION     DAOMGY010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46415_length_2489_cov_2.837716"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PLR57_04055"
     CDS             <1..895
                     /locus_tag="PLR57_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQQ40661.1"
                     /translation="RQVLDQGYYPDGIYTAPSVADLERDIDLALAFGFNGARPHEKVF
                     EERYLYYADRKGFLLWGEYPNWNCKTKPSNPVGLKHTLDEWQTVLERDYGHPSVIGWC
                     PLNEARFVWGNATDYTGQKALYDMTKAYDPARPVVGSSGGDLFVTDLHDAHFYTHDAR
                     TLKKLVRSGRYNEAPDFVYSIWRIFQNKLLKKKDLLKLPCYVSEYGGLSFLVEGKSWG
                     YHGKFTAEETFVQKYCELTNALFEEDCIGFCYTQLYDVEQEQNGLLRYDRSAKLSPAG
                     TEAIRRCNAQPKRQLTLNQAD"
     gene            942..2384
                     /locus_tag="PLR57_04060"
     CDS             942..2384
                     /locus_tag="PLR57_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ40662.1"
                     /translation="MEQTTKQTTKLGSRNWIGISLFAALGSVAWCMENDFLNLFIDRT
                     ISTNPLVISLIVSASAITAALTTMLVGIYLDRAGKRVRFMSWGYVIWGFVIMAFALIN
                     VKGISNTFGVSESTAVIACIVLVTLMDCIMTFFGSASNDAAFNAWVTDITNPTNRGKV
                     EGALGIFGFLGVSLVFVFDFLGGITYNHYFDASGNEVSNYVEGGSAVHGNWTLFFILI
                     GVLVLLAGLIGRFFVKDAPQLKPNPDSRMNKMLYGFRWSVVKQNKKLYTTLLGSSLLG
                     IAGMTSAPYLLIYMERTLGFKDYIIPYGILSIVSVAFGFLFGMYADKKGKKLRFILPG
                     MLVYILGCVIMFFGTTKQFASNAIFVFCAGSMVNGFGSAMIANVFGAKVRDYTPPDRV
                     GLFQGVRMVFNIMIPMCIGPVITALINNINKANTIGYDAAGNAIFNYSSSMFLFGAVL
                     VLLVLIPLGMLKKFEAKEAVLTPAAPPSGE"
BASE COUNT          539 a          716 c          679 g          555 t
ORIGIN      
        1 tcgtcaggtg ctcgaccagg gatattaccc ggacggaatc tataccgccc ccagcgtcgc
       61 cgacttggag cgggatatcg atcttgccct cgctttcggc ttcaatggcg cgcgcccgca
      121 cgaaaaggtg ttcgaagagc ggtacctgta ctacgccgac cgcaagggat tcctgctctg
      181 gggggagtat cccaactgga actgcaaaac gaagccaagc aaccccgtcg ggctgaagca
      241 cacgctggat gaatggcaga cggtgctcga acgcgattac ggtcatcctt ccgtgattgg
      301 gtggtgcccg ctcaacgaag cgcggtttgt ctggggcaac gctacggatt acacagggca
      361 gaaagcgctg tacgatatga ccaaggcgta cgatcccgcc cgccccgtag tcggcagcag
      421 cggcggcgac ctgtttgtga ccgatctgca cgacgcgcat ttctacacgc acgacgcgcg
      481 cacgctcaaa aagctcgtgc gcagcgggcg gtacaacgaa gcgcccgatt tcgtgtactc
      541 tatctggaga atcttccaaa acaagctgct caagaagaaa gacttgctca agctcccctg
      601 ctatgtttcc gaatacggcg gactttcctt cctcgtggaa ggaaaatcct gggggtacca
      661 cggaaagttc accgccgagg aaacgttcgt tcaaaagtac tgcgagctta caaacgcgct
      721 gtttgaggag gactgcatcg gcttttgcta tacccagctc tacgacgtgg agcaggagca
      781 aaacgggctt ttgcgctacg accgctccgc aaagctttcc cccgcgggaa cggaagccat
      841 ccggcggtgc aacgcgcagc caaaacggca gcttacgctg aatcaagcgg attaaccgca
      901 caattataga atcctgttca tcaaacgatt gggaggcagt tatggaacaa acaaccaagc
      961 agacaacgaa gctcggcagc aggaactgga tcggcatctc gctgtttgcc gctctgggca
     1021 gcgttgcctg gtgcatggaa aacgactttc tgaacctgtt tatcgaccgc acgatctcta
     1081 caaacccgct tgtgatctcg ctgatcgtca gtgccagcgc catcaccgcc gcgctgacga
     1141 ccatgctggt cggaatctac ctcgaccgtg ccggcaagcg cgtgcggttt atgagctggg
     1201 gctacgtgat ctggggtttt gtaatcatgg cctttgcgct catcaacgtc aaaggcattt
     1261 ccaacacgtt cggcgtgtcc gaaagcaccg ccgtgatcgc ctgtatcgtg ctggtgacgt
     1321 tgatggactg cattatgacg ttcttcggct ccgcttccaa cgacgcggcg ttcaacgcgt
     1381 gggttacgga catcaccaat cccaccaacc gcggcaaagt ggaaggcgcg ctgggtatct
     1441 tcggtttcct cggcgtaagc cttgtgttcg tgttcgattt tctcggcggc attacctata
     1501 accactattt tgacgcctcg ggcaacgagg tttccaacta tgtggaaggc ggctcggccg
     1561 tgcacggcaa ctggacgctg ttcttcattc tcatcggcgt actcgttctg ctggcaggct
     1621 taatcggcag gttttttgta aaggatgcgc cgcagctcaa accgaatccg gacagccgca
     1681 tgaataaaat gctctacggg ttccgctgga gcgtcgtcaa gcaaaacaaa aagctctaca
     1741 ccaccctgtt gggcagctcc cttctaggta tcgcggggat gacctctgcg ccgtatctgc
     1801 tcatctacat ggagcgcacg ctcggcttta aggattacat cattccatac ggcattctga
     1861 gtatcgtcag cgtggcgttc ggctttttat tcggtatgta cgccgacaaa aagggtaaaa
     1921 agctgcgctt catcctgccc ggtatgctgg tttacatctt aggctgcgtg atcatgttct
     1981 ttggcacgac aaagcagttt gcctccaacg ccatcttcgt gttctgcgcg ggatcgatgg
     2041 tcaacggctt tggcagcgcg atgattgcca acgtgttcgg cgcgaaggtg cgcgactata
     2101 cgccgcccga ccgggtcggc ctgttccagg gcgtgcggat ggtgttcaac atcatgattc
     2161 caatgtgcat cggccccgtg attaccgcct tgatcaacaa tatcaacaag gccaacacca
     2221 tcggctatga cgcggcgggc aacgccattt tcaactactc ctcctccatg ttcctcttcg
     2281 gcgcggtgct ggttctgctg gtgctgattc cgctcggcat gctcaagaag ttcgaagcaa
     2341 aagaggcggt tttaaccccc gccgctccgc cctccggcga ataattccgg aaccgggcgg
     2401 cagctaatct ccataaaaag ggcgctacca acatgctttt tagcacaaag gaagcgccct
     2461 gtttgtttgc gcttgaaccg tatgatcac
//
LOCUS       DAOMGY010000210         2488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_46443_length_2488_cov_2.842992, whole genome shotgun sequence.
ACCESSION   DAOMGY010000210 DAOMGY010000000
VERSION     DAOMGY010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46443_length_2488_cov_2.842992"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLR57_04065"
     CDS             complement(<1..844)
                     /locus_tag="PLR57_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ40663.1"
                     /translation="MTNIAVRLSGITKRFPGVVALKNVGFEIKEGEVHCLVGENGAGK
                     STLIKLLAGVEQADEGTITLFDKEVHFRTPEEALRAGVGVVYQELSNFVHLDVATNLN
                     ANDFPRKRGMIDYKALYRRTREHLDDCGLAYINEKSSMRELSLGTQQMVEIASLLGQE
                     AKVVILDEPTSALTEVEVEKLYELIARMKRQGVTIIYISHRLDEVMHLADTITVLKDG
                     EHVVTFPRDENTTKQMLVRYMVGRDVEFDYRLSDTVQTDVLLEAKGLSSGDKLMDASF
                     VLHKG"
     gene            complement(879..1868)
                     /locus_tag="PLR57_04070"
     CDS             complement(879..1868)
                     /locus_tag="PLR57_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40664.1"
                     /translation="MEKTSGSTKVSVAGVLRKILQTRELSLVVLLAIIVIALMSSTST
                     FATSGNIRVLLQGMSVDMMIAIPMAISLIAGNIDFSVGSNLCLSSAIVGLALNGGVPA
                     VLAVLIGLASGAFFGFLNGVIINKLGVTPLVATLGTWMAYRGIALVIIGGQTLGNFGE
                     SFTIIGRATWLGVPSTIVYMFAVILVGIFIVKYLSFFHNAYLIGSNKLSARLAGINIE
                     KFIYVSYMITGMIAAFAGIVLAARLGSSSQNAGTQLEFRNVVGLLVGGISMDGGEGKL
                     IGAVLGVTLMQIINNAIVLLYLNPSYTQVITGGILILAVAVDQLSKKRKVAAK"
     gene            complement(2045..>2488)
                     /locus_tag="PLR57_04075"
     CDS             complement(2045..>2488)
                     /locus_tag="PLR57_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40665.1"
                     /translation="VFTINQVHEHMQTKERKRGFLDMVKEYYPGIHPFELNIPDNSNL
                     YYEMVKAAIVDEGLRGLFCTDADSYIAGEVLRDLGRKDIVVVGFDLSEMGEQLMKEGY
                     LKVMIEQRPELFSYQAAGQCSTTFTIMKRRKRYSIRIWQSSPANA"
BASE COUNT          517 a          703 c          702 g          566 t
ORIGIN      
        1 cgcccttgtg cagcacaaag gaagcgtcca tgagcttgtc cccgctggaa agacctttgg
       61 cttccagcag cacgtccgtc tggacggtgt cgctcaggcg gtagtcgaac tccacgtcgc
      121 ggcctaccat gtagcgaacg agcatctgct tggtggtgtt ttcgtcccgc gggaaggtga
      181 cgacatgctc gccgtccttg agcacggtga tggtatccgc cagatgcatc acctcgtcca
      241 gccggtggga gatatagata atcgtcacgc cctgccgctt catgcgggcg atgagctcat
      301 agagcttttc cacttcgact tcggtgagcg cggaagtcgg ctcgtccaga atgacgacct
      361 ttgcctcctg accgagcagc gacgcaatct ccaccatctg ctgtgtgccg aggctgagct
      421 cgcgcatgga gcttttttcg ttgatatacg ccagcccgca gtcgtccaga tgctcgcgcg
      481 tacgccgata gagcgctttg tagtcgatca tgccgcgctt acgcggaaag tcgtttgcgt
      541 tgaggtttgt ggcaacgtcc agatgcacaa agttggagag ttcctgatac acaactccaa
      601 cgcccgcgcg cagggcttct tccggcgtgc ggaagtgcac ttccttgtca aagagcgtaa
      661 tggttccttc gtccgcctgc tccacgcccg cgaggagctt gatgagcgtg gacttccccg
      721 cgccgttttc acctaccagg caatgtactt cgccttcctt gatttcaaac ccgacgtttt
      781 tgagcgccac gacgcccggg aaacgcttgg tgatgccgga aaggcgcacc gcgatattcg
      841 tcattttctg cctccgatca taccggttgt ttccgcgcct acttggcggc gacctttctc
      901 tttttgctca gctggtcgac cgcgaccgcc agaatgagga tgccgcccgt aatcacctgc
      961 gtgtagctcg ggttgaggta gagcagcacg atggcgttgt tgataatctg catgagggtt
     1021 acgcccagca ccgcgccgat gagcttgcct tcgccgccgt ccatcgagat gccgccgacg
     1081 agcaggccga ccacgttgcg gaactccagc tgcgtgcccg cgttctggct gctgctgccg
     1141 aggcgcgccg ccagcacgat acccgcaaac gccgcgatca tgccggtgat catgtaggaa
     1201 acatagataa acttttcaat gttgatgccc gcgagccttg ccgaaagctt gttggagccg
     1261 atcaggtagg cgttgtggaa gaagctcagg tactttacga taaagatgcc aacgaggatg
     1321 accgcgaaca tatacacgat cgtggacggc acgccgagcc aggttgcgcg accgatgatc
     1381 gtaaagctct cgccaaagtt gccgagcgtc tgcccgccga tgatgaccag cgcgatgccg
     1441 cggtacgcca tccatgtgcc gagcgttgca accagcgggg tcacgccgag tttgttgata
     1501 atcacgccgt ttaggaagcc gaagaacgcg ccggacgcaa ggccgatgag tacggccaga
     1561 accgccggaa ccccgccgtt gagcgcaagg cctacgattg cgctggaaag gcagaggttg
     1621 gaaccgaccg aaaagtcgat gttgcccgca atcagcgaga tcgccatggg gattgcgatc
     1681 atcatatcga cgctcatacc ctgcagaaga acgcggatgt tgccggacgt cgcgaatgtt
     1741 gacgtggacg acatcaaagc gataacgatg atggccagca ggacgacgag cgaaagctct
     1801 cttgtctgca gaattttgcg gagaacgccc gcaacggata ccttggtact gccagatgtt
     1861 ttttccaccg gtttgccctc ttctctgaag tgtgtagttg atgtttcaaa atttccaggt
     1921 tggttatttt ttcgcccgtt gtttcagatg cgggcggctg aaccgagatt aataggaaga
     1981 aacgtttgta agaaaaagct gttttgttga tgccgtttgc accgttggac tctttaataa
     2041 tccatcaggc attcgctggt gatgattgcc agatccgaat actgtatctt ttccggcgtt
     2101 tcattatagt aaaggtagtt gaacattgcc cggcggcctg ataggaaaag agttccggcc
     2161 gctgctcaat catgaccttg aggtaacctt ccttcatgag ctgctcgccc atctccgaaa
     2221 ggtcgaagcc cacgaccacg atatccttgc gtcccagatc ccgcagcact tcgcccgcga
     2281 tataggagtc cgcgtctgtg cagaagagtc cgcgcaggcc ttcgtccacg atggcggcct
     2341 tgaccatttc gtaatagagg ttggagttgt ccggaatgtt cagctcaaac ggatgaatac
     2401 cggggtaata ctccttgacc atgtccagaa agccgcgctt gcgctccttg gtttgcatgt
     2461 gctcgtgcac ctgattgatc gtaaacac
//
LOCUS       DAOMGY010000211         2484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_46554_length_2484_cov_3.273364, whole genome shotgun sequence.
ACCESSION   DAOMGY010000211 DAOMGY010000000
VERSION     DAOMGY010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46554_length_2484_cov_3.273364"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2058
                     /locus_tag="PLR57_04080"
     CDS             <1..2058
                     /locus_tag="PLR57_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HQQ40666.1"
                     /translation="PLDLAAEDTRDAEQEKREKALRVMRIRLITAAVFALPILYIAMA
                     HMFPSLGVPLPAFMNPHSFPLVFALVQLFLSVPVMLAGGRFFRVGFKTLFKGAPNMDT
                     LVAIGTGSAFLYGVYATVLIYLGDFGFAQHLYFESAAVVITLVMVGKYLEAVSKSKTS
                     EAIKKLMSLKPKTAVVIKDGAELEVALDEVAVGDLVLVRPGAAFPVDGVVQDGASSVD
                     ESMLTGESLPVEKQPGSAVTGGSINGEGMLRFTVTHVGEDTALSKIIHLVEEAQGRKA
                     PIAKLADVISGYFVPAVIGVAVAAAVIWTLAGKDFNFVLNIFVTVLVIACPCALGLAT
                     PTAIMVGTGKGAELGVLIKGGEALETTHGIRAVVLDKTGTITQGKPEVTDIVLYSQLN
                     EAFVLAVAASAERGSEHPIARAIVSAAESRNLPLDQPDSFRAVPGRGIEAAVSGKSVL
                     AGSAKLLLEQGVDIAASSRDALALSGAGKTLMYIAIDGKLAALMAAADAVKPTSRRAV
                     AQLKALGLDVYMLTGDNAATAKAVAESVGIEHVLSDVLPDGKASEVQKLQAQGKRVAM
                     VGDGINDAPALVQADVGMAIGTGTDVAVESADVVLMRGDLGAVGAAVALSRATIRNIR
                     QNLFWAFAYNVIGIPFAAGVFYAFGGPLLTPVFAGAAMALSSVSVVTNALRLKRFHLK
                     KKD"
     gene            2085..>2484
                     /locus_tag="PLR57_04085"
     CDS             2085..>2484
                     /locus_tag="PLR57_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40667.1"
                     /translation="MKKIILIVFSLVLASVLLAGCAATDVILKYSPFSLDTIVEKFPA
                     IVTDNTQTDHYFTLSVDAETLLKVSNNFSLTDEDIAIQTPLKPFTDAGLNPAKLGAGY
                     RTDGASLYLVADFGAGTGVQSTITNALFESV"
BASE COUNT          480 a          777 c          746 g          481 t
ORIGIN      
        1 ccccttgacc tcgccgcgga agacacgcgg gacgccgaac aggagaagcg tgaaaaagcg
       61 ctccgcgtca tgcgtataag gctgattaca gccgcggtgt tcgcactgcc gattctttac
      121 atcgccatgg cgcacatgtt tccctcgctc ggcgtgccgc tgccagcgtt tatgaacccg
      181 cacagcttcc cgctcgtgtt cgcgctcgtg cagctgtttc tttcggttcc cgttatgctg
      241 gcgggcggac ggttcttccg cgtagggttc aaaacgctct tcaagggagc gccgaatatg
      301 gatacgctgg tcgccatcgg caccggcagc gcgtttctct acggagtcta cgcaaccgtg
      361 ttgatctatc tcggagactt cggttttgcg cagcacctct attttgagtc cgccgccgta
      421 gtcattacgc tcgtcatggt cggtaaatac ctcgaagccg tcagcaagag caagacttcc
      481 gaggcgatta aaaagctcat gagcctcaag cccaagaccg cagtcgtcat caaggacggc
      541 gcggagctgg aagtggcgct ggacgaagtc gccgtgggcg atctcgtgct cgtgcggccc
      601 ggagcggcgt ttcccgtgga cggcgtggtg caggacggcg cttccagcgt agacgagagc
      661 atgctcaccg gcgaatccct gcctgtggaa aagcagcccg gttccgccgt caccggcggc
      721 agcatcaacg gcgaaggcat gcttcgtttc accgttacgc atgtcggcga ggataccgct
      781 ctttccaaaa ttatccatct tgttgaagaa gcgcaggggc gaaaagcccc catcgcgaaa
      841 cttgcggacg tgatctcagg ctacttcgtg cccgcggtca tcggcgttgc ggttgccgcc
      901 gccgtaatct ggacgcttgc cggcaaagac ttcaacttcg tgctcaacat ctttgtgacc
      961 gtgctcgtga tcgcgtgtcc ctgcgcgctg gggctcgcca cgccgacggc catcatggtc
     1021 ggcaccggca aaggcgcgga gcttggcgtt ttgatcaagg gcggcgaggc gctggaaacc
     1081 acgcacggca tccgcgccgt cgtgctcgac aaaaccggca ccatcacgca gggcaagccg
     1141 gaagtaaccg acattgtgct ctattctcag ttgaacgaag cgttcgtcct cgcagtcgca
     1201 gcttccgccg aacgcggatc ggagcacccc attgcgcggg caatcgtatc cgcggccgaa
     1261 agccgcaatc tgccgcttga ccagccggat tccttccgcg cggttccggg gcgcggcatc
     1321 gaagccgccg tttccggaaa aagcgtgctc gcgggcagcg caaagctgct cctggagcag
     1381 ggcgtcgata tcgccgcatc ctccagggat gcgctcgcgc tctccggcgc aggcaaaacg
     1441 cttatgtata ttgccatcga cggcaagctt gcagcgctga tggccgccgc ggatgcggtc
     1501 aagcccacaa gcaggcgcgc tgttgcgcag ctcaaagcgc tcgggctcga cgtttacatg
     1561 ctcaccggag acaacgctgc caccgccaaa gcggtcgcgg aaagcgtcgg cattgagcac
     1621 gtgctttcgg acgtgctgcc ggacggcaaa gccagcgaag tgcaaaaact ccaggcgcag
     1681 ggcaagcgcg tcgccatggt gggcgacggc atcaacgacg cgcccgcgct cgtgcaggcg
     1741 gacgtcggca tggccatcgg caccggcaca gacgtcgcgg tcgaatccgc ggacgtggtg
     1801 ctcatgcgcg gggacctcgg tgcggtcggc gcggcggttg cgctttcgcg cgcgacgatt
     1861 cgcaacatcc gccagaacct cttttgggcg tttgcctata atgtcatcgg cattccgttc
     1921 gcggccggcg tgttctatgc cttcggcggc ccgctgctga cccccgtgtt cgcgggtgcg
     1981 gccatggcgc tgagctccgt ctccgtcgtg accaacgcgc tgcgtctcaa acggtttcac
     2041 ctgaagaaga aagactgacc ctggactcag aaaggaacag cgttatgaaa aagatcatat
     2101 taatcgtgtt ttcgctcgtg ctagcatcag ttttactcgc agggtgcgcc gcaaccgacg
     2161 tgatcctcaa atacagccct ttttctctgg acaccatcgt ggagaagttt ccggcaatcg
     2221 tgaccgacaa cacccaaacc gatcactact tcacgctctc cgtggacgca gaaacgctgc
     2281 tgaaggtcag caacaatttt agcctcacgg atgaagatat cgcaatccaa acgccgctga
     2341 agccctttac cgacgcgggg ctcaaccccg caaagctcgg cgcgggttac cgaacggacg
     2401 gcgcatcgct ttaccttgtc gctgatttcg gcgcgggcac gggcgtgcag agcacgatca
     2461 caaacgcctt gtttgagagc gtaa
//
LOCUS       DAOMGY010000212         2478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_46719_length_2478_cov_2.925712, whole genome shotgun sequence.
ACCESSION   DAOMGY010000212 DAOMGY010000000
VERSION     DAOMGY010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46719_length_2478_cov_2.925712"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="PLR57_04090"
     CDS             <1..1477
                     /locus_tag="PLR57_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQQ40668.1"
                     /translation="TTILAQQHYNTMCSRFADFPIKAACLSRFQTPKEREAIKRALAE
                     GKIDVIVGTHALLAKDVKYRSLGLLIIDEEHRFGVNHKEQIKALKRTVDVLTLTATPI
                     PRTLNMSMTGIRDISVIETPPEARYPVQTYVLEYTDALLKDAISRELARKGQAYVVYN
                     RVQSMETMARKIEQLLPEVRITVAHGQMGETQLEAAMLEFLEHRSDVLLCSTIIESGL
                     DISNVNTLIVIEADRMGLAQLYQLRGRVGRSSRIGYAYFTVQTGRVMNEKAAKRLMAI
                     REFTQFGAGFQLAMRDLEIRGAGSLLGAEQHGNISDVGYEYYVKLVRRAVDEQQGRPL
                     DPVTETSVDIPIDAHIPHDYVPSELMRLKAYRRIADIDGAETMMDVTEEFIDRYGEPP
                     ESVTNLMRISLVKAYAQRAYLESVSVRDGEAKLKFAESAPIDGGRLIAAVSNMEGARL
                     IASAPPSVEIRQKNADAASLTGKLPQFLYTIVHCVDADTGI"
     gene            1543..>2478
                     /locus_tag="PLR57_04095"
     CDS             1543..>2478
                     /locus_tag="PLR57_04095"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQQ40669.1"
                     /translation="MKNFKWKQMLAALLAVTLMLSVMGCALKDDATDTASSSNASSGD
                     AVDNNAVAVKIGDKYTITRGDIADQYDYMVQMYSYYGMTAPTEDADIESMQDSVIATL
                     VSDKIQLYEADLMGITLTDEEKANVEAQVEEEMKYYMDSFQSQAEGEGASDVDARTLE
                     IFQEQLDAAGMEMDVDGFRAYLSEQYTNDALKTALKAKVTEGVTATDDEIQAYYDENL
                     ASQKEAYDATPTDYLSAAESAQSGDGDPVLYAPAGYVRVRSISITPTSELSSDYDTLQ
                     SDLDTLAAQYGAAALDALAAKYAAQGTDPATASLDV"
BASE COUNT          568 a          711 c          740 g          459 t
ORIGIN      
        1 gactacgatt ctcgcgcagc agcactacaa caccatgtgc tcccggtttg cggactttcc
       61 catcaaggcc gcgtgtctct cgcgctttca aactccgaag gagcgcgagg cgattaaacg
      121 cgcgctcgca gaagggaaga tcgacgttat cgttggcacc cacgcgctct tggcaaagga
      181 cgtgaagtac cgttcgctgg gcttactgat catcgacgag gagcaccgtt tcggcgtgaa
      241 tcacaaagag cagatcaaag cgctcaaacg aaccgtggat gtgctcaccc tgaccgcaac
      301 gccgattccg cggacgctga atatgtccat gacggggata cgcgatatca gcgtgatcga
      361 aacgccgccg gaagcgcgct atccggtgca gacctacgtg ctggaataca cggacgcgct
      421 gctcaaggac gccatcagcc gcgagcttgc gcgaaaaggg caggcgtatg tggtctacaa
      481 ccgcgtgcag agcatggaaa ccatggcgcg aaagatcgag cagctcctgc cggaggtgcg
      541 cattaccgtc gcccacgggc agatggggga aacgcagttg gaagcggcca tgctggaatt
      601 tctggagcac cgttcggacg tgctgttgtg ctctacgatc atcgaaagcg ggctggatat
      661 ttcaaacgtc aacacgctga ttgtcatcga agcggatagg atggggcttg cccagctcta
      721 tcagcttaga ggccgcgttg ggcgttccag ccggatcggg tacgcgtact tcaccgtcca
      781 gaccgggcgg gtcatgaacg aaaaagccgc aaagcggctc atggcgattc gggaatttac
      841 gcagttcggc gcggggttcc agcttgccat gcgcgatttg gaaattcgcg gcgcgggttc
      901 gctgctcggc gcggaacagc acggcaacat ctccgacgtc gggtacgaat actacgtcaa
      961 gcttgtgcgc cgcgcggtgg acgagcagca ggggcgcccg ctcgacccgg tgacggaaac
     1021 cagcgtggat atccccatcg acgcgcatat tccgcacgac tacgtgccca gcgagctcat
     1081 gcgcctcaag gcttaccgca ggattgcgga tatcgacggc gcggagacga tgatggacgt
     1141 gaccgaagag ttcatcgacc gctacggcga gccgccggag agcgttacaa acctcatgcg
     1201 catttcgctg gtcaaggcat atgcccagcg ggcgtatctg gagagcgttt ccgtccgcga
     1261 cggcgaggca aagctcaaat ttgccgagtc cgctcccatc gacggcggaa ggctgattgc
     1321 cgccgtttcc aatatggaag gggcgcggct gattgccagc gcgccgccct ccgttgaaat
     1381 acggcagaaa aacgccgacg ccgcgtcgct caccggcaaa ctgccacaat tcctttacac
     1441 aattgttcat tgcgttgacg cggacacggg catataatga caagtgactt taactggctt
     1501 ttacgcgccg ggcgcggaaa ataaaaaagg gagaaatcaa acatgaagaa tttcaaatgg
     1561 aagcagatgc tggctgccct gctggcagtt acgctgatgc tgtccgtcat gggctgtgcg
     1621 ctcaaggacg acgcgaccga tacggcatcg agctccaacg cgagcagcgg agacgccgtt
     1681 gacaacaatg cggtcgccgt caaaattggc gataaataca ccatcaccag gggcgatatt
     1741 gcggatcagt acgactacat ggtgcagatg tactcctatt acggcatgac tgcgccgacc
     1801 gaggatgccg acattgaatc catgcaggac agcgtgattg caacgctggt ctccgacaag
     1861 attcagcttt acgaagcgga tctcatgggc atcacgctga ccgacgaaga gaaggccaac
     1921 gttgaagcgc aggtcgaaga agagatgaag tactacatgg attccttcca gtctcaggcg
     1981 gagggtgagg gcgcgtccga tgtagatgcg cgtacgctcg aaatcttcca ggaacagctc
     2041 gatgccgcag gcatggagat ggatgttgac gggttccgtg cttatctctc tgagcagtac
     2101 accaacgacg cgctgaaaac tgctctcaaa gcaaaggtca ccgaaggcgt taccgcgacg
     2161 gatgatgaga ttcaggctta ctacgatgaa aacctcgctt cccagaagga agcttacgat
     2221 gcaaccccga ccgattacct ctccgcggcg gaatccgccc agagcggcga cggcgatccg
     2281 gtgctctatg cgcccgcggg ctatgtccgc gtccgttcga tctccattac cccgacgagc
     2341 gagctttcgt ctgattacga cacgctgcag agcgatctgg atacgcttgc cgctcagtac
     2401 ggcgctgccg cgctggatgc ccttgccgcc aagtatgccg cgcagggcac cgaccccgca
     2461 accgcttctc tcgacgtt
//
LOCUS       DAOMGY010000213         2476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_46769_length_2476_cov_3.258984, whole genome shotgun sequence.
ACCESSION   DAOMGY010000213 DAOMGY010000000
VERSION     DAOMGY010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46769_length_2476_cov_3.258984"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PLR57_04100"
     CDS             complement(<1..562)
                     /locus_tag="PLR57_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HQQ40670.1"
                     /translation="MANKSRKYDLVIWDWNGTLLDDTETCYGIANFMRQERGMDLMPS
                     IDTYRTMFRFPVIEYYRSMGYTFETEPYDEVAREFIAMYAERVSLCPMQPCAEETLQA
                     VLDLGARQVLLSATGQQKLNEQVAMFSLNRYFERVIGGSDNLAHGKADYAREFLQECG
                     VTPRRALFIGDTDHDFEIASSIGCDCA"
     gene            complement(709..1737)
                     /locus_tag="PLR57_04105"
     CDS             complement(709..1737)
                     /locus_tag="PLR57_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HQQ40671.1"
                     /translation="MQHPLSAGPLLNAKGELNEAGYATSLVKTYDRSAIKAGKTRIKE
                     WDYYLIYNGSFGAALTIDDNSYMGMISASFLDFEAQKEKTVSPMFWLPMGKTNFPPSS
                     KTGDVEKTMKGAYASIKHENGGRRLICHLDKFDGDTPFDLNIFLFDEPRDSMVIATPF
                     AEQKTAFYYNQKIIGMSAIGTVKCFGETFTFELGDSFGLLDWGRGVWTHENTWYWGGG
                     QGLVEGHVVGFNIGYGFGDVTAASENMIFIDGIAHKLDRLTFNIPQKDGKDDFMSPWT
                     FTSSDGRFETAFTPVLDRAAYASVGPLLSDQHQVFGHMDGTLVLDDGSKITLKHFMAF
                     AEKVHNKW"
     gene            1925..>2476
                     /locus_tag="PLR57_04110"
     CDS             1925..>2476
                     /locus_tag="PLR57_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQQ40672.1"
                     /translation="MQRVFGAVLLAAAGAVMVWQLYTILILGMIHVASGLLLIAMVCV
                     PLFLGVFLLARSFTDPAAQRKVVRVSLAVLFGFYLAALASELILARIDFLHFSQAAAQ
                     YRENFDLMTNFRPFETVLLYLRALKYNYIGPGIPLSNLLGNMLLFMPMAVFLPCLFHT
                     MQKLWVFVLAMAGMLVMVEALQLL"
BASE COUNT          487 a          678 c          701 g          610 t
ORIGIN      
        1 gcgcgcagtc gcagccgatg gaggaagcga tttcaaagtc gtgatccgtg tccccgataa
       61 acagcgcccg gcgcggggta acgccgcatt cctgcaggaa ctcgcgggcg taatccgcct
      121 tgccgtgagc aaggttgtcg ctgccgccga taacgcgctc gaaatagcgg ttgagcgaaa
      181 acatcgcaac ctgctcgttc agcttctgct gacccgttgc ggagagcagc acctgccgcg
      241 cgcccagatc cagcaccgcc tgcagggtct cttccgcgca gggctgcatg gggcagaggg
      301 aaacccgctc ggcatacatg gcgataaact cgcgggcaac ctcgtcgtaa ggctcggttt
      361 caaaggtgta acccatgctg cggtaatact cgatcacggg aaagcggaac atggttcggt
      421 acgtgtcgat gctcggcatc aaatccatgc cgcgttcctg ccgcatgaag ttggcaatgc
      481 cgtagcaggt ctccgtgtcg tccaggagcg tgccgttcca gtcccatatg acgagatcgt
      541 atttgcggga tttgtttgcc atttcatgcg cctcgtttca aaccggacga acgccgtccg
      601 gttttttacc gcccatgata cacctttttg gagcggcagg gaacagttat tttcgcctta
      661 ccacttttta tgcgtcattt tttagcaaag agaagctttt ttcgggattc accatttatt
      721 gtgcactttt tcggcgaacg ccataaagtg cttgagggta attttgctgc cgtcgtcgag
      781 cacgagcgta ccgtccatat gcccgaacac ctgatgctgg tcggagagca gcgggccgac
      841 gctggcgtac gccgcgcggt cgagcaccgg cgtaaacgcg gtttcgaagc gcccgtcgct
      901 ggaggtgaac gtccaggggg acataaagtc gtccttgccg tccttttgcg gaatgttaaa
      961 cgtcagccgg tcgagcttat gcgcgattcc gtcgataaaa atcatgtttt cggaagcggc
     1021 tgtcacgtcg ccaaagccgt aaccgatgtt gaagccgacc acatgccctt caacgaggcc
     1081 ctgtccgccg ccccagtacc aggtgttttc gtgcgtccag acgccgcgcc cccagtcgag
     1141 caggccaaag gaatccccca gctcgaaggt gaacgtttcg ccaaagcact tgacggtgcc
     1201 gatcgcgctc atgccgatga tcttctggtt gtagtaaaac gcggtcttct gctcggcaaa
     1261 cggggtagcg attaccatgg aatcgcgcgg ctcgtcaaag aggaagatat tgagatcaaa
     1321 cggggtatcc ccgtcgaact tgtccaaatg gcaaatcagc cgccgtccgc cgttttcgtg
     1381 cttaatggaa gcataagcgc ccttcatggt tttttccacg tcgcccgttt tggagctcgg
     1441 aggaaaattt gtcttaccca tcggcagcca gaacatcggg gagacggtct tttccttttg
     1501 cgcctcaaag tcgagaaagg aagccgaaat catgcccatg taggagttgt cgtcaatggt
     1561 cagcgccgcg ccgaagctgc cgttgtagat caggtagtag tcccactcct tgatgcgggt
     1621 cttgccagcc ttaatagcag agcggtcgta agtttttacc agcgaagttg cgtagcctgc
     1681 ctcgttgagt tcgccttttg cgtttaaaag aggtccggcg gaaagcggat gctgcatgaa
     1741 tcaatacctt cctttcgtat cgctcgttcg atatggagtt gtatgctgtg ttcctttcat
     1801 tatacgcgtg ttgactttcc tctgcaagaa accgcccgtt tgaataagag gaattccggc
     1861 gtgcgatcgc tttggcacgc ggaaaagagt gtgatataat agcgggatga ttcagaagaa
     1921 aacattgcag cgcgtctttg gcgcggttct gctggccgcc gccggagccg tgatggtctg
     1981 gcagctttat acgattctca tcctcgggat gatccatgtc gcgtcggggc ttttgctcat
     2041 cgcgatggtc tgcgtaccgc tgtttttagg cgtgttcctg cttgcgcgct cgtttactga
     2101 ccccgccgcc cagagaaaag tcgtacgggt ttcgctggcg gtactgttcg ggttctacct
     2161 tgccgcgctg gcgagcgaac tgattcttgc cagaatcgac tttctgcatt tttcgcaggc
     2221 ggcggcgcag tatcgcgaga acttcgacct gatgacgaat ttccgtccgt tcgaaacggt
     2281 gcttttgtac cttcgcgcgc tgaaatacaa ctatatcggg cccgggattc cgctttccaa
     2341 ccttttgggc aacatgctct tgttcatgcc catggcggtg tttctgcctt gcctgtttca
     2401 tacgatgcag aagctctggg tgttcgtgct cgccatggca ggcatgctcg tgatggtgga
     2461 agcgctccag ctgctt
//
LOCUS       DAOMGY010000214         2474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_46842_length_2474_cov_2.821827, whole genome shotgun sequence.
ACCESSION   DAOMGY010000214 DAOMGY010000000
VERSION     DAOMGY010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46842_length_2474_cov_2.821827"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1343
                     /gene="thiI"
                     /locus_tag="PLR57_04115"
     CDS             171..1343
                     /gene="thiI"
                     /locus_tag="PLR57_04115"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HQQ40673.1"
                     /translation="MEPTLVVRYVEIHLKGLNRPYFERSLVKRIELALKPLKADVIRE
                     QGRIFVFGIPAEELDSAADKLTRVFGIHSVSPALATEKEWPEIVEAAKTLMEKRLSGK
                     EHLTFKVFARRSDKRFAMRSADINRTLGGLLLEAFPALSVDVHKPDCYVGVEIRQNQA
                     FIFIDELLGAGGMPVGCNGHAMLLISGGIDSPVAGHMIAKRGVRISAVHFFSYPYTSE
                     RARDKVVDLTRILSAYAGAVDLYLVPFTEIQLAIYEKCPQSETTVLMRRLMMKLSERL
                     ALSCGAQALVTGESIGQVASQTIESMTVTGDAVTLPVFRPLIGFDKEEIVDRAKAMGS
                     YETSILPFEDCCTVFVPQHPVTKPKLTDIRASEALVDFEPMMQKAVAETITLHIEP"
     gene            1509..1844
                     /locus_tag="PLR57_04120"
     CDS             1509..1844
                     /locus_tag="PLR57_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40674.1"
                     /translation="MKFIFQLIGVLVVLLILVMAVLFYLGDKGLLSGKLDQLITSLQL
                     LSKQAWVEIRYFMDDTGIAEDAAGLLDQGAEFLRESVEPRTTSKPGTEGAVTPTPEPS
                     PTPTTIVIG"
     gene            complement(1918..2379)
                     /locus_tag="PLR57_04125"
     CDS             complement(1918..2379)
                     /locus_tag="PLR57_04125"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HQQ40675.1"
                     /translation="MNRLDKCGYWLMLSDYDLGTIDALIAGRRWVYVAYLCQQAVERQ
                     LKAMYVYYIDSEPPKTHNVNFLFSKVVDSEPFKKEADQSRLAAGRIACEDYLMDAMFY
                     YMSDYPFSYKNISGRFVGEALALELYKKTKENVAWLRGFLPNVEIPQIPER"
BASE COUNT          544 a          707 c          669 g          554 t
ORIGIN      
        1 cgcgcggaag gcgcaatccg cttcagcttc tgcgcgttca acacgccgga ggagatggac
       61 gaaaccgcct ccgtcatagc tgaacaaatt gcgtttttac ggcggtttca gcgccgctaa
      121 agcataacat aacataaaag caagattact tacgataagg aaatggcaac atggaaccaa
      181 cactcgtcgt ccgctatgtg gaaattcatt taaaagggct caaccgcccg tactttgagc
      241 gcagcctggt caagcgcatc gagctcgcac tcaagccgct caaggcggac gtaatccgcg
      301 agcaggggcg tattttcgtt ttcggtatcc cagcggaaga gctcgacagc gcggcggaca
      361 aactcacgcg cgtgttcggc attcattccg tcagccccgc gctggcaacg gaaaaggaat
      421 ggccggaaat cgttgaagcg gcgaagacgc tcatggaaaa gcgcctctcc ggcaaggagc
      481 atctcacgtt caaggtattc gcgcgccgtt cggacaagcg ctttgccatg cgctcggcgg
      541 atatcaaccg taccctcggc ggcctgctgc tggaagcctt ccccgccctg tcggtggacg
      601 tacataagcc tgactgctat gtcggcgtgg agattcggca gaatcaggcg tttatcttta
      661 tagatgagct tctgggagct ggcggtatgc cggtcggctg caacggccac gccatgctgc
      721 tcatctccgg cggcatcgac agccccgttg cggggcacat gatcgccaag cgcggcgtgc
      781 gcatctccgc cgtgcacttt ttcagctacc cctacacgag cgaacgcgcg cgggataagg
      841 ttgtggattt gacccgtatc ctctcggctt acgctggtgc ggtcgatctg tatctggttc
      901 cgtttacgga aattcagctc gccatctacg aaaagtgccc ccagagcgag acgacggtgc
      961 tcatgcggcg gctcatgatg aagctctccg agcggctcgc tctctcctgc ggcgcgcagg
     1021 cgctcgtgac cggcgaatcc atcggtcagg ttgcaagcca gaccatcgag agcatgaccg
     1081 ttacagggga cgcggtgacc ctgcctgtgt tccgtccgct gattgggttc gacaaagagg
     1141 aaatcgtcga ccgcgccaag gcgatgggtt cgtatgaaac ctccattctg ccgtttgagg
     1201 actgctgcac ggtgttcgtg ccccagcacc cggtcaccaa accgaagctt acggatattc
     1261 gcgccagcga agcgctggtt gactttgagc cgatgatgca gaaggccgtt gcagagacga
     1321 ttacgctgca tatcgagccg taaccgttta tctcgctgaa gaaagcgaaa atcgacagaa
     1381 catcaaattc acacaacatt cacagcgcat gcctttaacg catggtataa tgcgcttgtg
     1441 aaggaaccgc ccaattcctc actttttcca gcgattacgc gttttttaga cggaggagat
     1501 gaacccagat gaaatttatc tttcagctca tcggcgtgct cgtcgtgctg ctcatcctcg
     1561 tgatggcggt gctgttttac cttggcgata aggggctgct cagcggaaag ctcgatcagc
     1621 tcattacctc tcttcaactt ttgagcaagc aggcatgggt cgaaattcgt tactttatgg
     1681 atgataccgg aatagccgag gacgcggcgg gacttctcga tcaaggggcg gagttcctgc
     1741 gggaatccgt cgagccgcgc acgaccagca aacccggaac ggaaggcgcg gtaacgccca
     1801 caccggaacc ctcgcctaca cccaccacga tcgttatcgg ctgacgacga gccaaaacaa
     1861 catcacgcgc aatcaaaaac gcttcccatg tgggaagcgt tttaaattac tctattgtta
     1921 ccgttcggga atctgcggaa tctcaacgtt cggcaggaat ccgcgcagcc aggcaacgtt
     1981 ttcctttgtc tttttgtaga gttccagcgc gagcgcttcg ccgacaaacc gaccgctgat
     2041 atttttgtag gaaaacggat agtcggacat atagtagaac atcgcgtcca tgaggtaatc
     2101 ctcgcaggcg atgcgccctg cggctaggcg gctctggtcc gcttcctttt tgaacggttc
     2161 gctgtccact accttggaga ataagaagtt gacgttgtgc gtctttggcg gttcggaatc
     2221 gatataatac acatacatcg ccttgagctg gcgttccacc gcctgctggc aaagataggc
     2281 aacgtatacc cagcgccttc cggcaatcag cgcgtcgatg gtgccaagat cgtagtccga
     2341 cagcatcagc caatatccgc atttatcgag tctgttcatc cggctcggtc tcctcttttt
     2401 cctctttctt aatcgtaccg ttgttcacaa gctgcctgag cgtttccgct accgcgccgt
     2461 cgaatatttc ttcc
//
LOCUS       DAOMGY010000215         2464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_47111_length_2464_cov_2.352013, whole genome shotgun sequence.
ACCESSION   DAOMGY010000215 DAOMGY010000000
VERSION     DAOMGY010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47111_length_2464_cov_2.352013"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLR57_04130"
     CDS             complement(<1..256)
                     /locus_tag="PLR57_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HQQ40676.1"
                     /translation="MEKPYEFVKEEKHLYQPKTVPELIEVPEMTFLAVDGRGNPNETD
                     GAYGKALELLYALSYTIKMSPKSGHAIPGYFEYRVPPLEGL"
     gene            complement(372..1001)
                     /locus_tag="PLR57_04135"
     CDS             complement(372..1001)
                     /locus_tag="PLR57_04135"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQQ40677.1"
                     /translation="MEEILFFSYVLVCVLISIVVVYAASRVVCRKKGLTRTRADWLKL
                     FVFAVYIAGVFYFTGTGTLYNIRQYGMGAGALQYSLVPFSAQSFDVTAYLLNIVLFLP
                     LGFLLPLIWPNYNRFWRILIFGAALSLLIELSQLLNIRSTDIDDLLLNTLGAALGFLL
                     FCLYQFVFHRKRKPVPQTTGSPVLYLAAMYASYFFLFNELGFAKLLYGF"
     gene            complement(1038..1856)
                     /locus_tag="PLR57_04140"
     CDS             complement(1038..1856)
                     /locus_tag="PLR57_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQQ40678.1"
                     /translation="MWIALAILVVLFFIWMVLVMPSRSSKAQRAPFQNRTFAHRGLFK
                     QDQSVPENSLLAFQHAVDAGYGVELDVQLTKDKVVVVFHDDDLKRVCGIDARVDAYTF
                     EELQAFPLFGSEHRIPTFTDVLGVLNGKIPVIVELKAGGDWKCLCELTLELLNGYKGD
                     YCVESFHPLLVRWFYLHAPQILRGQLSEAARFSKRYVPFYQAVMMSRLFTNVLTHPQF
                     IAYHIGPKCLSVRLSEALGAMKVAWTARPKDDHARLTRKNDAIIFEHYRPEPRF"
     gene            2009..>2464
                     /locus_tag="PLR57_04145"
     CDS             2009..>2464
                     /locus_tag="PLR57_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40679.1"
                     /translation="MKLNYKRTFLVGFAFLSICAFWQMYDTVIPLILRDTYSLPDGPA
                     GIIMGLDNVVALFLLPLFGSVSDGNRAPKWVWFAMLGAGIVTGAISWLTGSAATSAWL
                     VATLPWISGSLATIELICEILASICAAALIVLPLMTTLARASAKLGRRIP"
BASE COUNT          560 a          723 c          639 g          542 t
ORIGIN      
        1 agagcccttc cagcggcggc acgcggtatt cgaaatatcc cggaatcgca tgcccgctct
       61 ttgggctcat cttgatcgta tagctcaacg cgtacaaaag ctccagcgcc ttgccgtacg
      121 ccccgtccgt ctcgttcggg ttgccgcgcc cgtccacggc gagaaacgtc atctcaggca
      181 cctcgatcag ttcgggaacc gtcttcggct gatacaggtg cttttcttcc tttacaaact
      241 catacggctt ctccattttg ccgtttcctc ctttcgccga cgcaatcgac gatcattttt
      301 atgtatccat cctaacgcaa gaaacctgac agcagtgtgt caggttcctt atcaccgttt
      361 tatccggctg gctaaaagcc gtacagaagc tttgcaaagc cgagttcgtt aaacaggaaa
      421 aagtagcttg cgtacatggc cgcaaggtac agcacggggc tccctgtggt ttggggtacg
      481 ggcttccgct tccggtgaaa tacgaattgg tacaagcaaa acagcagaaa accgagcgcc
      541 gcacccagcg tgttgagcag caggtcgtcg atatccgtgc tgcggatgtt gaggagctgg
      601 cttagttcaa tcagcagaga gagcgcggct ccaaaaatga ggatgcgcca aaaccggttg
      661 taattcggcc agatcagcgg cagcaggaac cccagcggca gaaacagcac gatgttgagc
      721 agatacgccg tcacgtcaaa cgactgcgcg gaaaaaggaa caaggctata ttgcagcgcg
      781 cccgcgccca tgccgtactg gcggatgtta tacagcgttc ccgtgcctgt aaagtagaat
      841 acaccggcga tataaaccgc aaacacgaat agtttcagcc aatccgcgcg cgttcgggtc
      901 agccccttct tccggcatac aacgcgagaa gcggcataca cgaccacaat cgaaatcagc
      961 acacaaacaa gtacgtaact gaaaaacagg atttcttcca ttcttactct ttggctctcc
     1021 ttattgaata agagaaacta aaaccgcggt tccgggcgat agtgctcaaa gatgatcgca
     1081 tcgtttttgc gcgtgagcct ggcgtggtcg tctttggggc gcgccgtcca ggcaaccttc
     1141 atcgcgccca gcgcttcgga aagccttacg gaaaggcact taggtccgat atggtacgca
     1201 atgaactgcg gatgcgtgag cacgtttgta aacagtctgc tcatcataac cgcctggtaa
     1261 aatggcacat accgcttgga gaagcgcgcg gcttcggaaa gctgtccgcg caggatctga
     1321 ggcgcgtgga ggtaaaacca gcgcaccagc agcgggtgga agctctccac gcagtagtcg
     1381 cccttgtagc cgttgagcag ttccagcgtg agctcgcaga ggcatttcca gtcgccgccc
     1441 gccttcagtt cgacgatcac cgggattttg ccgttcagca cgcccagcac gtccgtaaac
     1501 gtaggaatcc tatgctccga accgaacagc ggaaacgcct gcagctcttc aaacgtatac
     1561 gcgtccacgc gcgcgtcaat tccgcagacg cgcttgagat cgtcgtcatg aaacaccacg
     1621 actactttat ccttcgtcag ctgtacgtcc agctccacgc cgtagccggc gtccacggcg
     1681 tgctgaaacg caagaagcga attttccggc acggactgat cctgtttaaa cagcccgcgg
     1741 tgcgcgaacg tcctgttttg aaacggtgcg cgctgggctt tggaactccg cgagggcata
     1801 acaagaacca tccagatgaa gaacaaaacg actaaaatcg caagcgcgat ccacataatc
     1861 cacaatgcct ccctgtgctt tttgcgacga ttttccaata aaccgtattg tcattatacg
     1921 ccggatgcgt taccatgtat atatctgttc gggaaccgct cccgaacgta ttcttccttt
     1981 catattatta gaaatcggag aagtgcatat gaaattgaac tacaaacgaa cgttcctcgt
     2041 cgggttcgcg tttctctcca tctgcgcgtt ctggcagatg tacgacacag tcatcccgct
     2101 gatcctgcgg gacacctact cgctgccgga cggccccgcc ggtatcatca tggggttgga
     2161 taacgtcgtg gcgctcttcc tgctgccact gttcggttcg gtcagcgacg gcaaccgcgc
     2221 gcccaagtgg gtttggttcg ccatgctcgg tgcgggtatc gtcaccggcg caatcagttg
     2281 gctgaccggt tccgcggcaa cttccgcctg gctcgttgca accctgccct ggatttccgg
     2341 ctcgcttgct accattgagc tgatctgcga aatccttgcc agtatctgcg cggcggctct
     2401 tatcgttctc ccgctcatga ctacgctggc gcgcgcaagc gcaaaactcg gccgacgcat
     2461 tccg
//
LOCUS       DAOMGY010000216         2459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_47233_length_2459_cov_3.283278, whole genome shotgun sequence.
ACCESSION   DAOMGY010000216 DAOMGY010000000
VERSION     DAOMGY010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47233_length_2459_cov_3.283278"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /gene="obgE"
                     /locus_tag="PLR57_04150"
     CDS             complement(<1..888)
                     /gene="obgE"
                     /locus_tag="PLR57_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQQ40680.1"
                     /translation="MQFIDHARIVIKAGNGGDGCVAFHREKYVAQGGPSGGDGGKGGD
                     LIFVADQQLSTLLDFKFQRFYRAENGEPGKAKLSSGRDGAPLIVRVPVGTVVRDVETG
                     AILADLNEDSKERVLLRGGYGGKGNARFATPTRQTPDFSQPGLKTAEHEVELELKSIA
                     DVGLVGLPSVGKSTILSVLTAAKPKIAAYHFTTLSPNLGVASRHGRSFVLADIPGLIE
                     GAAEGAGLGHDFLRHVERTRMLVHVLDISGSELRDPLDDYEKINDELEKFSPALLQVP
                     QLVAANKTDLPQANIDKVKR"
     gene            complement(910..1575)
                     /gene="nth"
                     /locus_tag="PLR57_04155"
     CDS             complement(910..1575)
                     /gene="nth"
                     /locus_tag="PLR57_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQQ40681.1"
                     /translation="MMLTKAQKTEALAILARTYPNAKPALEFHTPFELLVATILSAQS
                     TDVMVNLCTRELFPVANTPEQIAAMTEEELFPYIKRCGFFNTKGRHILETARILVTKY
                     GGEVPADLELLQTLPGVGRKTANVVASNAFGIPAIAVDTHVFRVSNRIGLANAKNVEK
                     TEEQLQKAIPREHWSQAHHWLIFHGRQICAARKPKCEICPLIDLCEFYKKNKAENSTK
                     QPS"
     gene            complement(1572..>2459)
                     /locus_tag="PLR57_04160"
     CDS             complement(1572..>2459)
                     /locus_tag="PLR57_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQQ40682.1"
                     /translation="YCDYLVEQSEFRFEGLRIVLDCANGASSFIAKDVFERLGAEVIA
                     KSCAPDGININDHCGSMHPERLQEMVVNYGADLGFAFDGDADRLISCDEYGNIVDGDQ
                     AMGILALHMLARGTLKHNTLVLTVMSNLGLKNALRDAGIQIVETKVGDRYVLESMLQN
                     DYAIGGEQSGHIILLSRNTTGDGMMSAIALLGVVAESKKRLSKLAAKIPKFPQVLVNV
                     TVENERKAEALADADMAARTQEIEKHLGKNGRVLVRASGTEPLVRIMLEGQDEKEILD
                     LALSLAHIIVNKHNGKIRT"
BASE COUNT          426 a          714 c          757 g          562 t
ORIGIN      
        1 gcgtttgact ttatcaatgt tggcttgcgg taaatcggtt ttgttggcgg cgaccagctg
       61 ggggacctgc agcaaggcgg gggaaaattt ctccagctcg tcgttgatct tttcgtagtc
      121 gtccaacggg tcgcgcaatt cgctgccgga gatgtccagc acatgcacaa gcatacgcgt
      181 ccgctccacg tgccgcaaaa agtcgtgccc caatcctgcg ccttccgccg cgccttcgat
      241 caggccgggg atatccgcca gcacaaacga acgcccgtgc cggctcgcca cgccgaggtt
      301 cggcgaaagg gtggtaaagt gatacgcggc gatcttgggt tttgctgcgg tcaaaacgga
      361 gaggatcgtg ctcttgccaa cggagggcag gccgaccaga ccgacgtccg caatgctctt
      421 aagctccagt tccacttcgt gttccgccgt tttgagcccc ggctgggaaa agtccggcgt
      481 ctgccgcgtc ggggtggcaa agcgcgcgtt gcccttgccg ccgtatccgc cgcgcaggag
      541 cacgcgctcc ttgctgtctt cgtttaagtc ggcgaggata gcgccggttt ccacgtcccg
      601 cacgacggtg ccgacgggaa cgcggacgat cagcggcgcg ccgtcgcgcc cggacgagag
      661 cttcgccttg ccgggttcgc cgttttccgc gcggtagaac cgctggaact tgaaatccag
      721 cagggtggaa agctgctgat cggcaacaaa aatcaagtcg ccgcccttgc cgccgtctcc
      781 gccggagggg ccgccctggg caacgtattt ttcgcgatgg aacgcaacgc atccgtcccc
      841 gccgtttccg gctttaatga cgatgcgcgc atggtcgatg aattgcatgt gtttttctct
      901 tttccggcct tatgaaggct gttttgtgga attctccgct ttgtttttct tgtaaaactc
      961 gcagagatca ataagcgggc agatttcgca tttcggcttc cgcgcggcgc agatttgccg
     1021 tccgtgaaag atcagccagt gatgcgcctg cgaccagtgc tcgcggggaa tcgctttctg
     1081 cagctgctct tccgtttttt ccacgttctt ggcgttcgcc agcccgatgc ggttgctcac
     1141 gcggaagacg tgagtgtcca cggcgatggc ggggatgccg aacgcgttgc tcgcaacgac
     1201 gttggcggtc tttcgaccca cgccgggcag ggtttgcagc agttccagat ccgcaggaac
     1261 ctctccgcca tacttggtta cgaggatgcg cgcggtttcg agaatatgcc gtcctttggt
     1321 gttgaagaag ccgcagcgct tgatgtaagg aaagagttct tcttccgtca tggcggcaat
     1381 ctgttccggt gtgtttgcaa cggggaagag ttcgcgcgtg cagaggttca ccatcacgtc
     1441 cgtactctgc gccgagagaa tggttgccac gagcagttca aacggcgtgt ggaactccag
     1501 cgcgggcttt gcgttggggt atgtccgcgc gaggatggcg agcgcttcgg ttttttgcgc
     1561 tttggtgagc atcaggtgcg aatctttcca ttgtgtttgt tcacgatgat atgcgccagc
     1621 gaaagcgcca gatcgagaat ttccttttcg tcctgacctt ccagcataat gcgcacgagc
     1681 ggttctgtgc cggaggcgcg gacgagcacg cggccgttct tgccgaggtg cttttcaatt
     1741 tcctgcgtgc gcgcggccat atccgcgtcc gcaagggctt ccgctttgcg ctcgttttcc
     1801 actgtaacgt tgaccagcac ttgcgggaat ttcggaatct tggcggcgag cttgctcagg
     1861 cgctttttgc tctcggcaac aacgcccaac agcgcgatgg cgctcatcat gccgtcgccg
     1921 gtggtgttgc gcgaaagcag gatgatatgc ccgctctgct cgccgccgat ggcgtagtcg
     1981 ttctgcagca tgctttcaag cacgtaccgg tcgccaacct tggtttccac aatctggata
     2041 cccgcgtcgc gtagggcgtt tttcaggccg agattgctca tgaccgtcag cacgagcgtg
     2101 ttgtgcttga gcgtgccgcg cgcgagcata tgcagcgcga gaatacccat ggcctggtcg
     2161 ccgtctacga tgttgccgta ttcgtcgcag gagatcagcc ggtcggcgtc gccgtcgaaa
     2221 gcgaatccca aatccgcgcc gtagttgaca accatctcct gcagccgttc ggggtgcatg
     2281 gagccgcagt ggtcgttgat gttgatgccg tcgggcgcgc aggacttggc gatgacttcc
     2341 gcgccgaggc gttcgaacac gtcctttgcg ataaacgaag aagcgccgtt ggcgcagtcc
     2401 agcacgatgc gcaggccttc aaagcggaac tcggactgct cgaccagata gtcgcagta
//
LOCUS       DAOMGY010000217         2450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_47517_length_2450_cov_3.513152, whole genome shotgun sequence.
ACCESSION   DAOMGY010000217 DAOMGY010000000
VERSION     DAOMGY010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47517_length_2450_cov_3.513152"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLR57_04165"
     CDS             complement(<1..603)
                     /locus_tag="PLR57_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40683.1"
                     /translation="MLPKKPLPALIVILLDLIGIGVMLLIFTLFHHMLDLDSGEPLQN
                     IVFGATPTPTVEAVATEEPTLAPQATATPQITATPAPTPTPEPGDFSATFPTDEPNVE
                     NAIGVYKTDTLRVVVTEGHTDDAVYYVADVWIRNIREFKTAFAKDKFGRGLYEMPDTI
                     AEHNGAVLAITGDTYGSSSQSVVIRNGNLYRDNGDGDVCIL"
     gene            complement(891..1610)
                     /gene="deoD"
                     /locus_tag="PLR57_04170"
     CDS             complement(891..1610)
                     /gene="deoD"
                     /locus_tag="PLR57_04170"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQQ40684.1"
                     /translation="MKSANITPHIGCSPEDFAKTVLMPGDPLRAKFIAETYLSDYELV
                     NNVRGVQGYTGYFEGKRVSVMASGMGMPSMGIYSFELFNIFGVETIIRIGSAGGMHPD
                     LKLFDIVLGQGACTNSNYFSQYGLNGSYAPIADYGTLRKAVDECEKRSLRYKVGNLLS
                     SDMFYSEKGIEGTLEWAKLGVLAVEMEAAALYMNAARAGKKALAICTISDLLIGGEVT
                     TAEQRQSAFHDMMQVALSIAE"
     gene            1776..>2450
                     /locus_tag="PLR57_04175"
     CDS             1776..>2450
                     /locus_tag="PLR57_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013853277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HQQ40685.1"
                     /translation="MPKSFIPQGYHSKLGLYDTQNAIEVIKSTFLESLSAVLRLKRVS
                     APLFVNPAEGLNDNLNGVERPVSFDIPSTGGNGEVVQSLAKWKRYALGRYGFRPGKGL
                     VTDMNAIRRDEALDNLHSVYVDQWDWEKVITPEERTEETLKQTVLSIVDAIYYTQQVI
                     CAKYDALTPFVRRDVTFITAQELLDLHPNLDPKEREYRFVKEHPTTFVMGIGGKLTDG
                     KPHDGRA"
BASE COUNT          577 a          658 c          690 g          525 t
ORIGIN      
        1 aagaatgcag acgtccccgt cgccattgtc gcggtaaaga ttgccgtttc gaatcacgac
       61 gctctggctg cttgacccgt aggtatctcc cgtaatggcg agaaccgcgc cgttatgctc
      121 cgcaatggta tcgggcattt catataaccc gcgtccgaac ttatccttgg caaacgcggt
      181 cttaaactcg cggatgttgc ggatccatac gtcggccaca taataaacag cgtcatcggt
      241 gtgtccttct gtcacgacaa cgcggagggt atccgttttg taaacgccga tggcgttttc
      301 cacgttcggt tcgtccgtgg ggaacgttgc ggaaaaatcg ccaggttccg gcgtcggcgt
      361 cggcgcgggc gttgcggtaa tctgcggcgt tgcggtggcc tgcggcgcaa gcgtcggttc
      421 ttcggtcgca acagcctcaa cagtcggcgt gggcgttgcg ccaaatacga tgttttgcag
      481 cggttcgccg ctgtccagat cgagcatatg atgaaacaac gtgaaaatca aaagcatcac
      541 gccgatgcca atcagatcca gcagaataac aatcagggcg ggcaagggtt ttttcggaag
      601 catggcgcgt cctttcaaca ggtcaaatcc gataagacgc aagtaacgac ttatcggaaa
      661 ggatgcttag atgatatccg cgtgacttta aaaaagcaat cgttgacgcg gaaagcaaca
      721 ataaattcac aaatgttggg aagtattgac aaattgatgc caggtttccg cgtaaccgcc
      781 tgtataatga aacgccgggg cttttgagct ccccggcgtg tctgcggtaa atataagaga
      841 aaaatccggc atgccccggg cgaaaagccg gggcgaacca ggcgaaacct ttattccgcg
      901 atggagagag caacctgcat catatcgtgg aaggcgctct ggcgctgttc cgcggtggtg
      961 acttcgccgc cgatgagcaa atcggaaatg gtgcagatgg caagcgcttt tttgccagcg
     1021 cgcgcggcgt tcatatagag cgcggcggct tccatctcca cggcgagaac gccgagcttt
     1081 gcccactcca gcgtgccttc gatgcccttt tcggaataga acatgtcgct cgaaagcagg
     1141 ttgccgacct tatagcgaag gctccgcttt tcgcattcgt ccacggcctt gcgaagcgtt
     1201 ccgtaatccg caatcggcgc gtagctgccg ttcaggccgt actgcgagaa gtaattggag
     1261 tttgtgcagg cgccctggcc gagaacgatg tcgaacagct tcaaatcggg atgcatgccg
     1321 cccgccgaac cgatgcggat aatggtttcc acgccgaaaa tattgaacag ttcaaacgaa
     1381 tagataccca tggacggcat gcccatgccg ctggccataa cggaaacgcg cttgccctcg
     1441 aaatatcccg tgtagccctg aacgccgcgc acgttgttga cgagctcgta atcgcttaaa
     1501 tacgtctcgg cgatgaactt ggcgcggagc ggatcgccgg gcattaaaac ggtttttgca
     1561 aagtcttccg gactgcagcc gatatgcggc gtgatgttgg cgctcttcat aagtgaatac
     1621 ctccgaaaag ttgttgcttg attcataaat tcagcctgtt tgagtatatc ataacaggca
     1681 ccgaattgca cggcgggaca aagcacttga catccttttt tgcgctggac taaactgtct
     1741 taacgaaaca cgatctggct ggaaggagcg aaacaatgcc taaatcattt atcccgcagg
     1801 ggtaccattc caagcttggc ctgtacgata cgcagaacgc gatcgaggtc atcaagagca
     1861 cctttttaga atctctctcg gctgtgctgc gtctcaagcg cgtctccgcg ccgctgttcg
     1921 tcaaccccgc cgaggggctg aacgacaacc tcaacggcgt cgagcggccg gtctcgtttg
     1981 acataccttc cacaggtggt aacggagagg tcgttcagtc gctcgcaaaa tggaagcgtt
     2041 acgcgctggg cagatacggc ttccggcccg gcaaggggtt ggtaacggac atgaacgcca
     2101 tccgccgtga cgaagcgctg gataacctcc attcggtcta cgtggatcag tgggattggg
     2161 aaaaagtaat cacgcccgaa gagcgtacgg aggaaacgct gaagcaaacc gtgctgagca
     2221 tcgtggacgc catctattat acgcagcagg tgatctgcgc aaagtacgac gcgctcactc
     2281 cgtttgtgcg ccgggacgtt acgtttatta ccgcgcagga actgctcgat ctgcacccga
     2341 atctcgaccc gaaggagcgc gaataccgct tcgtcaagga acatccgact acgtttgtca
     2401 tgggaatcgg cggaaaactg accgacggaa agccgcacga cgggcgcgcg
//
LOCUS       DAOMGY010000218         2446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_47626_length_2446_cov_3.111669, whole genome shotgun sequence.
ACCESSION   DAOMGY010000218 DAOMGY010000000
VERSION     DAOMGY010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47626_length_2446_cov_3.111669"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLR57_04180"
     CDS             complement(<1..635)
                     /locus_tag="PLR57_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HQQ40686.1"
                     /translation="MNSQDLHTHSVWDDGQNTLEEMANAAVAANLDAIGFSGHSPLPY
                     RNRWALQADRLEAYKAEALRLKKLFAGNIDIYLGIEWDVMSTIPPTGFDYVIGSVHHV
                     TVNGTIYSADATPETVETCLSDAFHGDADASARAYFTQLPALAKIPEVDIIGHFDLPT
                     KFNETHGFYNEDSPAYTAAALEAMEALVKADKIFEINTGAISRGYRTTPYPS"
     gene            complement(632..1282)
                     /gene="hisIE"
                     /locus_tag="PLR57_04185"
     CDS             complement(632..1282)
                     /gene="hisIE"
                     /locus_tag="PLR57_04185"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HQQ40687.1"
                     /translation="MLELDKLKFDEKGLIPAVVIDTNTKQVLMLAYMNAESLRISMEE
                     GRTCFWSRSRQSLWRKGETSGNVQHIVSITADCDADTLLVEVKPDGPACHTGAESCFF
                     QPVFAGETPKGFAYEDLMELLRGRKANPKEGSYTTYLFEKGVDKILKKIGEETTEVIL
                     AAKDGDRANMVYEICDLMYHVMVLMVETGVELDEVKREMASRHIVEHKIKQEKMVE"
     gene            complement(1285..2046)
                     /gene="hisF"
                     /locus_tag="PLR57_04190"
     CDS             complement(1285..2046)
                     /gene="hisF"
                     /locus_tag="PLR57_04190"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQQ40688.1"
                     /translation="MITKRIIPCLDVKDGRVVKGVNFLGLGDVSDPVTLARYYSESGA
                     DELVFYDITASAERRALFTDVLTRVAETIFIPLTVGGGINTLEDFDRVLKCGADKVSV
                     NSGAIKNPRLIEEAAKRYGDQCVVLSVDAKRVNGQFRVFAKGGREDTGLDALEWIKRG
                     VESGAGEIVLNSIDTDGVRTGFDLEMLRAVSELVGVPVVASGGAGGIADFITLFQTLP
                     KVDAGLAASIFHFGEVTIPELKNALADAGITVRRN"
     gene            complement(2092..>2446)
                     /locus_tag="PLR57_04195"
     CDS             complement(2092..>2446)
                     /locus_tag="PLR57_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HQQ40689.1"
                     /translation="EAIAVGVDLNDGLVAVKGWTETSGLSGLAFCKNLEEIGVQTVIC
                     TDIQKDGAMNGTNRALYQTLSESFSMRFIASGGVSSMEDILALKELNLYGAIVGKAYY
                     TGAIDLKAAILAAKE"
BASE COUNT          459 a          743 c          672 g          572 t
ORIGIN      
        1 gaaggatagg gcgttgtgcg gtaaccgcgc gaaatcgcac ccgtgttgat ttcaaaaatc
       61 ttgtcggctt taaccagcgc ttccatagct tccagcgcgg ctgcggtata cgccggggaa
      121 tcctcgttgt aaaagccgtg cgtttcgttg aactttgtcg gcaggtcgaa atgcccgatg
      181 atgtcgactt cgggaatctt tgccagcgcg ggcagctgcg taaaatacgc ccgggcggaa
      241 gcgtccgcgt cgccatggaa cgcgtcgctt aaacaggttt ccaccgtttc gggcgttgcg
      301 tccgcggagt agatggtccc gtttaccgta acgtgatgca ccgagccgat cacataatcg
      361 aaccccgtcg gcgggatggt actcattacg tcccattcga tgccgaggta aatatcaata
      421 tttcccgcga acagcttttt gagccgcaga gcctccgctt tgtaagcctc cagccggtct
      481 gcctgcagcg cccagcggtt gcggtagggc agcggggaat gcccgctgaa gccgattgcg
      541 tcgagatttg cggcgacggc cgcgtttgcc atttcttcga gcgtgttctg tccgtcgtcc
      601 caaacggagt gcgtgtgcag atcctgcgaa ttcattccac cattttctcc tgcttgattt
      661 tgtgctcaac gatgtgcctt gaggccatct cgcgcttgac ttcgtccagc tcaacgccgg
      721 tttccaccat cagcaccatc acatgataca ttaaatcgca gatttcataa accatattcg
      781 cacgatcccc gtcttttgcg gcgagaatca cctcggtcgt ctcctcgccg atcttcttga
      841 gaatcttatc gacgcctttt tcaaacagat acgtcgtata cgagccttcc ttcggattcg
      901 cctttctgcc gcgcaggagc tccatcaaat cctcataggc aaagcctttg ggcgtttccc
      961 ccgcaaagac cggctgaaaa aagcagcttt ccgcgcccgt atggcaggcg gggccgtccg
     1021 gtttgacttc gacgagcagc gtgtccgcgt cgcagtcggc ggtgatggag acgatgtgct
     1081 ggacgtttcc gctcgtttcg cccttgcgcc agagcgattg ccgcgagcgc gaccagaaac
     1141 aggttcgccc ttcctccatg gagatgcgca ggctttcggc gttcatatac gcaagcatga
     1201 gcacctgctt tgtgttggta tcgatgacga cggcgggaat cagtcccttt tcgtcgaatt
     1261 tcagcttgtc gagttccagc atggtcagtt cctccgcacc gttatgcccg cgtccgcgag
     1321 cgcgtttttg agttccggta tcgttacttc gccaaagtgg aagatcgagg cggctaagcc
     1381 cgcgtccacc ttgggcagag tttgaaaaag tgtaataaaa tccgctatcc cgcccgcgcc
     1441 gccggaagcg acgaccggca cgccaacgag ttcgcttacc gcgcgcagca tttcgaggtc
     1501 gaaccctgtc cgcacgccat ccgtatcgat ggagttgagc acgatttcgc ctgcgcccga
     1561 ttccacgccg cgcttgatcc actccagagc atccagcccc gtatcctcgc gcccgccttt
     1621 ggcgaatacg cggaattggc cgttgacgcg ttttgcgtcc accgagagca ccacgcactg
     1681 gtcgccgtag cgcttggcgg cttcttcgat tagacgcggg ttcttgatcg cgcccgagtt
     1741 gacgctgact ttgtccgcgc cgcacttgag cacgcggtcg aaatcctcca gcgtgttgat
     1801 gccgccgccg acggtaagcg ggatgaagat ggtttcggca acccgcgtga gcacgtccgt
     1861 aaacagcgcg cggcgttccg ccgaggcggt gatgtcgtag aacaccagct cgtccgcgcc
     1921 gctttcggaa tagtaccgcg ccagcgtcac ggggtcggaa acatccccga ggccgaggaa
     1981 gttgaccccc ttgacgaccc tgccgtcttt tacgtccaga cacgggataa tgcgtttggt
     2041 aatcatggta tgtcctcgcg gctgaaaagc ctgttctttg tgggttccat cttactcttt
     2101 tgccgccaaa atggcggctt tcaggtcgat tgcccctgtg taatacgctt tgccgacaat
     2161 cgcgccgtag aggtttaatt ctttgagcgc aaggatgtcc tccatcgaac tgaccccgcc
     2221 ggaggcgatg aaccgcatgg aaaacgactc gctgagcgtt tgatagagcg cgcggtttgt
     2281 gccgttcatc gcgccgtcct tttgaatatc cgtgcagata acggtttgaa cgccgatttc
     2341 ctccagattc ttacagaacg ccagccccga tagtccgctc gtctccgtcc agcccttgac
     2401 cgcgacaagc ccgtcgttca ggtcaacccc gacggcaatc gcttcg
//
LOCUS       DAOMGY010000219         2444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_47697_length_2444_cov_2.732105, whole genome shotgun sequence.
ACCESSION   DAOMGY010000219 DAOMGY010000000
VERSION     DAOMGY010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47697_length_2444_cov_2.732105"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLR57_04200"
     CDS             <1..690
                     /locus_tag="PLR57_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HQQ40690.1"
                     /translation="DIVIHSATKFIGGHGTAICGVIVEGGKFDWKASGKFPWISEPNP
                     SYHGLSFADVASPTAFATYLRAIVLRDTGATLSPFHSFLFLQGLETLSLRVERHVENA
                     LKIVKYLHRHPQVEAVHHPSLKSEPSHKLYEKYFPNGGGSIFTFEIKGTEEDAKAFID
                     NLDIFSLLANVADVKSLVIHPASTTHSQLSPAELLDQGIKPNTIRLSIGTEHADDLIE
                     ALENAFKAVKK"
     gene            complement(785..1300)
                     /locus_tag="PLR57_04205"
     CDS             complement(785..1300)
                     /locus_tag="PLR57_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HQQ40691.1"
                     /translation="MEINEIFELVPQAAEQLPRGAFLTAEGDCWNPMTIGWAQFGVIW
                     GKPIVTVLVRKSRHTFGLMEQTDVFTVSVPKSGELTKALAFCGSKSGRNVDKERESGL
                     TRLPARAGGADGVKECGSFFECRIVNKRMLDLDELDPELRSRYYGANQALPNGDPHML
                     YFGEVLGAYKI"
     gene            complement(1339..1644)
                     /locus_tag="PLR57_04210"
     CDS             complement(1339..1644)
                     /locus_tag="PLR57_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40692.1"
                     /translation="MKIWAKILKNHKIINEAVREFDSARPSDAEGWRPIVVALCKPLD
                     LECPVILKKHVAELQRFSRTVFSQGDFIDGVSFDHFELEIFPEKKKETRVEYIFGDE"
     gene            complement(1650..>2444)
                     /locus_tag="PLR57_04215"
     CDS             complement(1650..>2444)
                     /locus_tag="PLR57_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40693.1"
                     /translation="TPDPSASPDAEATTDPEATTEPEATEAVPTDDAAIPAVTGDGET
                     ITIEARKNNCVTARKVITVEPYVVQNLSFMVTNELATLSSDTGSVTVTGTATPGTQIT
                     AACPSTDVTFGVATVSETGTFSLAVSIGKVGAFDITLTGKLEGYYDGTAIATVERPPT
                     LDWSKFRKASSDLSKNYEKVSTGVTTSGDFYCTGTVTEIIATEPYAIFRIKLSDGSEV
                     ICANRSTKSTINSSDVKDKKEVIGTLKGFYTDGTTPYLWCWFIRNK"
BASE COUNT          512 a          718 c          643 g          571 t
ORIGIN      
        1 gatatcgtta tccacagcgc gacaaagttt atcggcggcc acgggactgc tatctgcggg
       61 gtcatcgtcg agggcggcaa attcgactgg aaagcctccg gcaagttccc ctggatttct
      121 gaaccgaacc cgagctatca cgggctgagt tttgcggacg tagcgtcccc caccgcgttt
      181 gccacgtacc ttcgcgccat cgttctgcgc gatacaggag ccacgctctc tccgttccat
      241 tccttcctct tcctgcaggg gctcgaaacg ctgtcgctcc gcgtggagcg gcacgtggaa
      301 aacgcgctca aaatcgtcaa atacctgcac aggcacccgc aggtcgaggc cgtgcaccat
      361 ccttcgctca agagcgaacc gagccacaag ctctacgaaa agtattttcc caacggcggc
      421 ggctctattt tcacctttga aatcaaggga acggaggagg acgcaaaggc gtttatcgac
      481 aacctcgata tcttttcgct gctggcaaac gttgcggacg tcaagtcgct ggtcatccac
      541 cccgcctcca cgacgcactc tcagctcagc cccgctgagc tgctcgatca gggcatcaaa
      601 cccaacacga tccgcctttc gatcggcacc gagcacgcgg acgatctgat cgaagcgctg
      661 gaaaacgcgt ttaaggcagt gaaaaagtaa aaaatcgaaa taaatattaa gaaaagcctg
      721 aggctttcag atcacctcag gctttaacat tttcagcttt atggctgcat attgggtaaa
      781 ttcgttaaat cttatatgcc ccgagcactt ctccaaagta aagcatgtgc ggatcgccat
      841 tcggcagagc ctgattggca ccgtagtacc ggcttcgtag ttccgggtcg agctcgtcaa
      901 gatcgagcat gcgcttgttg acgatgcggc attcaaagaa cgagccgcac tcctttacgc
      961 cgtccgcgcc gcccgcgcgc gcaggtaagc gggtcagtcc cgactcgcgc tctttatcga
     1021 cgttacggcc cgacttggag ccgcagaacg caagcgcttt tgtcagttca cccgacttgg
     1081 gaacgctgac ggtaaagacg tccgtctgct ccatcaaacc gaaggtatgg cggctctttc
     1141 gcaccaaaac ggtcacgatc ggcttgcccc agataacgcc gaactgtgcc cagccgatgg
     1201 tcatcgggtt ccagcaatcg ccttccgcgg ttaaaaatgc gccgcgcggc agctgctccg
     1261 ccgcctgcgg caccagttcg aatatctcgt taatctccat tgcttctacc tcctgcgctt
     1321 cggatggatt ttggcgcttc attcgtcgcc gaagatgtac tccacgcgcg tttccttctt
     1381 tttctccgga aagatttcca gttcaaagtg gtcgaacgat acgccgtcga taaagtcccc
     1441 ctgcgaaaac accgtgcgcg aaaaccgctg cagttcggcc acgtgttttt tcaggatcac
     1501 cgggcattcc agatcgaggg gcttgcagag cgcaaccacg atcggacgcc agccttccgc
     1561 atccgaaggc cgtgcggaat caaattcccg cacggcctcg ttgatgattt tatggttttt
     1621 gagtatcttt gcccaaatct tcacgttggt tacttgtttc gaatgaacca acaccagagg
     1681 taaggcgttg ttccatccgt atagaatccc ttcagcgtgc caataacttc tttcttatct
     1741 ttaacatccg acgagtttat cgtcgacttc gtgctgcgat tagcgcagat cacttcactg
     1801 ccgtcgctga gtttgatacg gaagatcgca taaggctcgg tcgcaataat ttctgtgaca
     1861 gtgccagtgc aatagaagtc accgctcgtg gttacgccag tcgatacttt ctcgtaattc
     1921 ttcgataaat cgctgcttgc ctttctaaac ttcgaccagt caagtgtcgg cggacgctcc
     1981 accgttgcaa ttgcggtgcc gtcataatac ccttccagct tgccggtgag ggtgatatcg
     2041 aacgcgccga ccttgccaat ggaaaccgca agcgagaagg ttccggtttc actgaccgtc
     2101 gctacgccga acgtcacgtc cgtcgagggg caggctgccg taatctgcgt gccgggggtc
     2161 gccgtgccgg taacggttac gctgccggta tcggaagaga gcgttgcaag ctcgttggta
     2221 accatgaacg agaggttctg caccacgtac ggttcaacgg tgatcacctt gcgggcggtc
     2281 acgcagttgt tcttgcgcgc ttcgatggta atggtttcgc cgtcgcccgt cactgcgggg
     2341 attgcagcgt cgtccgtcgg aacggcttcg gtcgcttccg gttcggtggt cgcttcagga
     2401 tccgttgttg cttccgcatc cggggacgcg gaaggatctg gcgt
//
LOCUS       DAOMGY010000220         2441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_47775_length_2441_cov_3.551970, whole genome shotgun sequence.
ACCESSION   DAOMGY010000220 DAOMGY010000000
VERSION     DAOMGY010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47775_length_2441_cov_3.551970"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..745)
                     /locus_tag="PLR57_04220"
     CDS             complement(53..745)
                     /locus_tag="PLR57_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HQQ40694.1"
                     /translation="MNDTLASALISLQYLARVALAAVLAGVIGYEREHVQRNAGMRTH
                     ILVAIGASLVMCCGEFLVTKYSGVMNLDPARLGAQVISGIGFLCAGTIIKEGISVRGL
                     TTASSLWCVSCIGLAAGAGAYLLAVGVAVLALIVLRVLRSLAHSRRKKSNRLVMQLVL
                     SMEGNTLISLQEDLQRMGCVIEEFSFAIDTDQQKRHVYLHLMLPNREDLSPLMEGLSR
                     IDGVVSVESVYE"
     gene            complement(1021..>2441)
                     /gene="asnB"
                     /locus_tag="PLR57_04225"
     CDS             complement(1021..>2441)
                     /gene="asnB"
                     /locus_tag="PLR57_04225"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004065131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HQQ40695.1"
                     /translation="LQTTASPLRNGDAVVCNGELYGFRKMRDALFSGYDFKSGSDCEL
                     LLPMYQRYGTDMFAMLDAEYALIIYDHKTDSLIAAGDPIGIRPLFYGYDVDGNILFAS
                     EAKNLTGLVETVNPFPPGHYWKDGEFVQYASLTDVSDYYKDDLDAVCGNIREKLTLGV
                     VKRLDADVPVGFLLSGGLDSSLVCAIANKYLPHPIKTFSIGMRDDAIDCKYARQVADY
                     LGCEHTEVIVTPQDAIGALRTVVRILETYDITTVRASVGMYLVCKYIHENTNIRVLLT
                     GEISDELFGYKYTDFAPNEEEFQKEAKKRIDEIYMYDVLRADRCISANSLEARVPFGD
                     LDFVKYVMTVDPALKMNRFGKGKYLLRRAFEGDYLPQDILYREKAAFSDAVGHSMVDQ
                     IKAYAEKLYTDEEFAARSAKYTFAKPFTKESLLYRELFEAEYPGQAHMVKDFWMPNRS
                     WEGCNVNDPSARVLSNYGASAV"
BASE COUNT          535 a          696 c          686 g          524 t
ORIGIN      
        1 tgcagacagc attccggctc attacatcct gttttgcggg taaagttctg ctttattcgt
       61 agaccgactc taccgagact actccgtcaa ttctggagag tccttccata aggggcgaca
      121 agtcttcgcg gttcggcagc atcaggtgca gataaacatg ccttttttgc tggtctgtat
      181 cgatggcaaa gctgaactct tcaataacgc atcccattct ctgcagatcc tcctgcaggg
      241 agatcagcgt gttgccttcc atagagagca cgagctgcat aacaaggcgg ttgctttttt
      301 tgcgccggct gtgcgcaagg ctccgaagca ctctaagcac gatcagcgcc agcaccgcaa
      361 ccccgacagc cagcagatac gcccctgcgc ccgcggcaag gccgatgcac gaaacgcacc
      421 aaaggctgct cgccgtggtc agcccgcgca cggaaatgcc ttccttgata atcgtgcccg
      481 cacagagaaa gccgatgccg ctgatgacct gcgcgccgag ccgcgcgggg tctaggttca
      541 tcacgcccga atatttggta acgagaaact ccccgcagca catcacaaga gacgcgccga
      601 tagccacgag aatatgcgtg cgcatgcccg cgttgcgctg aacatgctcg cgttcgtagc
      661 cgattacacc cgccagcacg gcggccagcg ccacgcgcgc cagatattgc agggaaatca
      721 acgcgcttgc taaagtatcg ttcattgaga aaagctcctt tccgtacagg ggacttggga
      781 gatcagcata tacgccaacc ggtccgggta tatatacccc ggatgcgttc gacggttaaa
      841 atagtattct atctgttttg ttaagctatt gtaaagcgca gatcaagatt tcgtcaagcg
      901 attgattgtg aataaaacaa accggcctcc ccgatgttca ggcgggagga ggccggttca
      961 aacatgccgt gaaaggagcg aatttccacg gcactcgagg atatcgcgtt aaagaagtag
     1021 ttaaaccgcg cttgcgccgt agttcgaaag cacgcgcgcg gaggggtcgt tgacgttgca
     1081 gccttcccag gagcggttcg gcatccagaa gtccttcacc atatgcgcct ggccggggta
     1141 ctccgcctca aagagctcgc ggtagagcag agactccttg gtgaacggct ttgcgaacgt
     1201 gtactttgcg ctgcgggcgg caaattcctc gtccgtatag agcttctcgg cataagcctt
     1261 gatctgatcg accatcgagt gcccgacggc gtccgagaac gcggcctttt cgcggtagag
     1321 aatatcctgc ggcagatagt cgccttcaaa cgcgcggcgc agcaggtact tgcccttgcc
     1381 gaagcggttc atcttgagtg cgggatcaac cgtcatgacg tacttaacga agtcgaggtc
     1441 gccaaacggc acgcgcgctt ccagcgagtt agcggagatg cagcggtcgg cgcggagcac
     1501 gtcgtacata tagatttcgt cgatgcgctt cttggcttcc ttctggaact cctcttcgtt
     1561 gggcgcgaag tccgtgtatt tatacccgaa cagctcgtcc gaaatctcgc ccgtaagcag
     1621 cacgcggatg ttggtgtttt cgtggatata cttgcagaca agatacattc caacgctcgc
     1681 gcgaacggtc gtaatatcgt acgtttcaag gatgcgcaca acggttctga gcgcgccgat
     1741 ggcgtcctgc ggggtaacga taacttccgt gtgctcgcag ccgagatagt ccgccacctg
     1801 gcgggcgtat ttacagtcga tggcgtcgtc ccgcataccg atggagaacg tcttgatcgg
     1861 gtgcggcagg tacttattgg cgatggcgca gaccagcgaa ctgtccagtc cgccgctgag
     1921 caaaaagccg accggcacgt ccgcatccag acgcttgacc acgccgagcg tcagcttttc
     1981 gcggatgttg ccgcagaccg cgtccagatc gtccttgtaa taatcggaaa cgtccgtcag
     2041 gctggcgtac tgaacgaact cgccgtcctt ccagtagtgt cccggcggga agggatttac
     2101 ggtttccacg agccctgtga gattttttgc ctcgctagca aagaggatgt tgccgtccac
     2161 atcgtagcca tagaacagcg gacggatgcc gatggggtcg cccgcggcga tcagcgagtc
     2221 cgttttatgg tcgtagatga tgagcgcgta ctccgcgtcc agcatggcga acatatccgt
     2281 tccatacctt tgatacatgg gtaagagcag ctcgcaatcc gagccgctct taaagtcata
     2341 tcccgaaaat aatgcatcgc gcatcttgcg gaagccgtag agttcaccgt tgcagaccac
     2401 cgcgtccccg ttgcgcaacg gggacgcggt ggtctgcaac g
//
LOCUS       DAOMGY010000221         2441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_47782_length_2441_cov_2.713747, whole genome shotgun sequence.
ACCESSION   DAOMGY010000221 DAOMGY010000000
VERSION     DAOMGY010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47782_length_2441_cov_2.713747"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PLR57_04230"
     CDS             <1..1006
                     /locus_tag="PLR57_04230"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HQQ40696.1"
                     /translation="VLTPRGEIIDLPVGSTPLDFAYRIHTNIGHHCQGAKVNGSLVRL
                     DYKLKTNDVVEIVTSNSQAGPSRDWLKIVKTQSARNKIRQWFKKENREENIARGRDML
                     EDAAKRQSQTLAELMKPEYTSVVLDRMRFTCMEDLYSAIGFGGISAGNVLHKLMELKK
                     KSDKVEELAEKIRSGEGGEAVKAHVPVNGIIVKGDPGMAVRFGNCCNPLPGDPIIGYI
                     TRGRGVSVHRADCPNVSSLRTEEDRIIDVEWAINTRSAFNASLQIEGENRPGTLSQIS
                     ATLSGLNISIQSINGRPVADGRYIIEMTFEVANTEQLNVIIKNIKKLKCVSEVYRMNR
                     "
     gene            1047..1493
                     /gene="dtd"
                     /locus_tag="PLR57_04235"
     CDS             1047..1493
                     /gene="dtd"
                     /locus_tag="PLR57_04235"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HQQ40697.1"
                     /translation="MRAVVQRTTAASVTVENEVIGEIGTGLVVFLGVEEGDTEQDLTY
                     ICEKVLGLRVFDDANEIPNMSVEDVNGSILLVSQFTLLADARKGRRPSYIRAARPETA
                     IPLYEEAIRKFSARVTVAHGVFQAYMQVKLVNDGPHTILLDSHKTF"
     gene            1525..2169
                     /locus_tag="PLR57_04240"
     CDS             1525..2169
                     /locus_tag="PLR57_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ40698.1"
                     /translation="MLFKQIINGSLGENAYLIAADGQKTCAVVDPGEAEPVLAALKAE
                     DLGCEAILLTHGHFDHIGGVRKLKDATGAKIYIHEADAGMLTSNRMSLAVLIGSMIPS
                     AEPDVLLQGGETLSIAGLNIRVIHTPGHTKGGICFAIEDEGVLLTGDTLFHTDAGRTD
                     FPGGSAKDLYHSIVDGLYTLEGDYTVYPGHEESTTLDEERKNNLFTTAGARRGW"
     gene            2163..>2441
                     /locus_tag="PLR57_04245"
     CDS             2163..>2441
                     /locus_tag="PLR57_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40699.1"
                     /translation="MVGIRTNRSEYSNDIAEEIRLFLGLTEIEPAEGEDDDKFELVFK
                     LLLNNNDNRFTIQAGAGAFRADDAFTIPADASALLVKKIEKRKLKLACY"
BASE COUNT          606 a          666 c          700 g          469 t
ORIGIN      
        1 cgtgctcacg ccgcgcgggg agattatcga cctgccggtg ggctcgacgc cgctcgactt
       61 tgcctaccgc atccatacga acatcggcca tcactgccag ggcgcaaaag tcaacggctc
      121 tctcgtgcgg cttgactata aactcaaaac gaacgacgtc gtcgaaatcg taacctcaaa
      181 cagtcaggcg ggaccgagcc gcgactggct caaaatcgtc aaaacgcaga gcgcgcggaa
      241 taaaatacgc cagtggttca aaaaagagaa ccgcgaggaa aacatcgcgc gcggcaggga
      301 catgctggag gacgcggcaa aacgccagag ccagacgctt gcggaactga tgaagccgga
      361 gtataccagc gtcgttctcg accgtatgcg ctttacttgc atggaagacc tgtattccgc
      421 catcggcttt ggcggcatat cggccggcaa cgtgctccat aagctcatgg agctcaaaaa
      481 gaagagcgat aaggttgaag aactggctga gaaaattcgc tccggcgagg gcggggaagc
      541 ggtcaaggcg cacgttcccg tcaacggcat tatcgtaaaa ggtgatccgg gtatggcggt
      601 gcgcttcggc aactgctgca atccgctgcc gggcgacccg atcatcggct atattacgcg
      661 cggcagaggc gtttccgtcc accgggcaga ctgtccgaac gtttcgagcc tgcgtacgga
      721 agaagatcgc atcatcgatg ttgagtgggc aatcaacact cgcagtgcgt tcaacgcgtc
      781 gctccagatt gaaggcgaaa accgccccgg cacgctttca cagatttccg caacgctttc
      841 cggcctcaac atcagcatcc agtccatcaa cggcaggccg gttgcggacg ggcggtatat
      901 cattgaaatg acgttcgagg ttgccaatac cgagcagctc aacgtgatta tcaagaatat
      961 caaaaagctg aagtgcgtga gcgaagttta tcgaatgaac cggtagctgc gtatttcacc
     1021 gtataaaaaa gaacaggcag gagcttatga gagcagtcgt acagcgaaca accgcggcgt
     1081 cggtaaccgt agaaaacgag gtcatcggcg agatcggaac cggtctcgtc gtgtttttag
     1141 gcgtggaaga aggcgacacg gagcaggatt tgacctacat ctgcgaaaaa gtgctcggcc
     1201 tgcgcgtttt tgacgatgcc aacgaaatac cgaatatgtc cgttgaggac gtcaacggca
     1261 gcatcctgct cgtttcgcag tttacgcttc tggccgacgc gcgcaaaggc cgccgcccca
     1321 gctatatccg cgcggcgcgt cccgaaacgg cgattccgct ctacgaggaa gccattcgga
     1381 agttcagcgc gcgcgtgacg gttgcgcacg gtgtgtttca ggcgtatatg caggtcaagc
     1441 tcgtcaacga cggcccgcat accatcttgc tggattcgca taaaacgttc tagcatccgg
     1501 cagttcccca aggaggaaga taccatgttg tttaaacaga tcatcaacgg ttcgctcggc
     1561 gaaaacgctt acctgatcgc ggcggacggg caaaaaacct gcgccgtcgt cgatcccggc
     1621 gaagcggagc cggttcttgc cgcactcaaa gcggaagacc ttggctgcga ggcgattttg
     1681 ctgacgcacg ggcatttcga ccatatcggc ggcgtgcgaa agttgaagga tgctaccggc
     1741 gcgaaaatct acatccacga agcggacgcg ggcatgctca cgagcaatcg catgagcctt
     1801 gcggtgctta tcggctctat gattcccagc gcggaaccgg acgttttact gcaaggcggc
     1861 gaaacgctct ccatcgctgg gctgaatatt cgcgtgattc atacgcccgg acacacgaag
     1921 ggcggcattt gctttgcaat agaagacgaa ggcgtgctgc ttacggggga cacgctgttt
     1981 cataccgacg cgggacggac ggactttccc ggcggcagtg caaaggactt gtaccattcc
     2041 atcgtggatg ggctctacac attagagggc gactatacgg tctaccccgg ccatgaggaa
     2101 tccaccacgc tcgacgaaga gcgcaaaaac aacctgttta caacggcagg agcgcggcgc
     2161 ggatggtagg cattcgaacc aaccgttctg aatacagcaa cgacatcgcc gaagaaatcc
     2221 ggcttttttt aggattgacc gaaattgaac ccgccgaagg agaagacgac gacaagtttg
     2281 aactggtgtt caaactgctt ttgaataaca acgacaatcg ctttacgatt caggcaggcg
     2341 cgggcgcatt tcgtgccgac gacgcattta cgattccggc ggatgcttcc gctctgctgg
     2401 ttaaaaagat agaaaagcgc aagcttaagc tcgcctgcta c
//
LOCUS       DAOMGY010000222         2440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_47809_length_2440_cov_1.728721, whole genome shotgun sequence.
ACCESSION   DAOMGY010000222 DAOMGY010000000
VERSION     DAOMGY010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47809_length_2440_cov_1.728721"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..1503
                     /locus_tag="PLR57_04250"
     CDS             322..1503
                     /locus_tag="PLR57_04250"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HQQ40700.1"
                     /translation="MLQTHLIAELLDMEAYDFEGVEQNEKRILLNVRMKRRTQECPAC
                     QTPCDQVHDYRLQRVKDSPIQGKMVIWQYRKRRYRCPCCGKRFYESNYLLPKRHRITN
                     RLAALGIDQLRRKQSRKEIANTLGVSESSVGRWMNLLEFGKPDRLPKVLSIDEFRGNT
                     EYGKFQCVLTDPVHKRIVDILPTRYHAQIYEYLRTFSSRNQVQYFVMDMNREYLDIAR
                     KLLPNAQIVIDRFHVVRYCTWALENVRKRVQTKLLPEQRKYFKRSRKLLLAHMRDLSA
                     ENKAVVERMLLIQCDLREAYLLKEKFYDFMDSSDSLQAKERLRAFRAHAFVADLPEFA
                     ACLTMLKNWEPFILNAFDCPYSNGFTEGVNNKIKVLKRIAFGYHSFKNFRTRILCTNN
                     A"
     gene            complement(1650..>2440)
                     /locus_tag="PLR57_04255"
     CDS             complement(1650..>2440)
                     /locus_tag="PLR57_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HQQ40701.1"
                     /translation="DDPELLELVEMEIRELLSTYEFPGDEIPIVKGSALLAMEALLEG
                     KKALETPECKCLFDLMDAVDSYIPTPERAVDKPFLMPVEDVFTITGRGTVATGRVERG
                     TVKVGDTVEIVGLAEATRSVVVTGVEMFRKQLDQAIAGDNIGALLRGVQRTDIERGQV
                     LAKPNSIHPHTHFMGRVYVLTKEEGGRHTPFFSGYRPQFYFRTTDVTGSIELPAGVEM
                     VMPGDHIDMTIQLITSIAIEPGLRFAIREGGRTVASGVVTEVIA"
BASE COUNT          593 a          655 c          636 g          556 t
ORIGIN      
        1 acgcgatcgg cacggcgaat aggtaatgca gcccacagat ttcctttagc agcagcagcg
       61 cggcgtagtc cgcggcgaac cccagaaagg ctgccagtcc atagcgcagc gcttgcgtga
      121 gcatccgcgc gtcttcccga attgcaaaga aaccttcgtt cttcttttcc atacgcgtag
      181 tatagcatac gttcggccgg ttggcgcaga taaaaaggct ctacactata gattggggta
      241 aggtaaaaaa agaagaagca taaccgcttc aaatcttgta gattaaggtt gttcacacca
      301 ctacgagaaa aggagaaggt tatgcttcaa acacatctta tcgcagaatt gctggatatg
      361 gaagcctatg attttgaagg agtggaacaa aacgaaaaac ggattctgtt aaacgtgcgg
      421 atgaagcgac gaacacagga atgtccggca tgccaaacac cttgcgatca ggtgcacgac
      481 tatcggctgc aacgtgtcaa agacagcccg attcaaggga aaatggttat ctggcagtat
      541 cgaaagcgac gatatcgctg tccctgctgc ggtaagcgtt tctatgagag taactatctt
      601 ttaccgaagc gtcatcgaat tacgaacagg ctggcggctt tagggatcga tcaactgcga
      661 aggaagcagt cccgaaaaga gatcgcaaac acgctcggag tatccgaatc ttccgttggg
      721 cgatggatga atctgctgga gttcgggaag ccggatcgat tgccaaaggt tctctccatt
      781 gacgaatttc gagggaatac cgagtatggg aagtttcagt gtgtcctgac cgatccggtg
      841 cacaaacgaa tcgttgatat cctcccaacg cgctatcacg ctcagattta tgagtatttg
      901 aggacttttt caagcagaaa tcaggtgcaa tatttcgtga tggacatgaa tcgggagtat
      961 ctcgatattg cgagaaaact gctgccaaac gctcaaatcg tgattgatcg gtttcacgtt
     1021 gtccgatact gcacctgggc actggagaac gtaagaaaga gagtacagac gaagttgctg
     1081 cccgaacagc gcaaatactt caagcgcagc agaaaactgc tgcttgcgca catgcgcgac
     1141 ctgtccgccg agaacaaggc tgtggtggaa cgtatgctgc tgatacagtg cgacctgaga
     1201 gaagcatatt tgctgaaaga aaagttctat gactttatgg attcgtccga ttccttacag
     1261 gcaaaagagc gtctccgcgc ttttcgcgcg cacgctttcg ttgcagacct gcccgagttt
     1321 gccgcatgcc ttacgatgtt aaagaactgg gagcctttta tcctcaacgc tttcgattgc
     1381 ccttattcca atggttttac cgaaggtgtc aacaacaaga tcaaagtact caaacgaatc
     1441 gccttcggtt accacagctt caagaatttt cgtactcgaa ttctctgtac gaacaacgct
     1501 taagggagct gttcgctctc gctcccagct ccctttgttt ctcttagtct actgctccac
     1561 cccaactatt gacggagagc ctctttaaaa aagaaaggtc aaaacacaaa gagaggctcc
     1621 ttgcggaacc tctcttgcgt tactctcgtt tacgcgatga cttcagtgac gacgccggag
     1681 gcaaccgtac ggccgccttc gcgaatcgcg aagcgcaggc ccggttcgat cgcgatcgac
     1741 gtgatcagct gaatcgtcat gtcgatgtgg tcgccaggca taaccatctc aacgcctgcc
     1801 ggcagctcga tggagcccgt cacgtccgtc gtacggaagt agaactgcgg acgatagccc
     1861 gagaaaaacg gcgtatgacg gccgccttct tctttcgtca acacgtacac gcggcccatg
     1921 aagtgcgtgt gcggatggat cgagttcggc ttcgccagaa cctggccgcg ctcgatgtcc
     1981 gtgcgctgta cgccgcgaag cagtgcgccg atgttgtcgc ctgcgatcgc ctgatccagc
     2041 tgcttacgga acatttctac gccggtcacg acgaccgagc gggttgcttc cgcgagaccg
     2101 acgatttcga ccgtgtcgcc gaccttgacc gttccgcgct ccacgcggcc cgtcgcgacc
     2161 gtaccacggc ccgtgatcgt gaacacgtct tctaccggca tcaggaacgg tttgtcaacc
     2221 gcgcgctccg gcgtcgggat gtagctgtct accgcgtcca tcaggtcgaa caggcacttg
     2281 cactccggcg tttccagagc ctttttgcct tccagcagcg cttccatcgc cagcagcgcg
     2341 ctgcctttga cgatcgggat ttcgtcgccc gggaattcat acgtgctcag cagttcgcgg
     2401 atttccatct cgaccagctc aagcagttcc ggatcgtcga
//
LOCUS       DAOMGY010000223         2438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_47859_length_2438_cov_3.099454, whole genome shotgun sequence.
ACCESSION   DAOMGY010000223 DAOMGY010000000
VERSION     DAOMGY010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47859_length_2438_cov_3.099454"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /gene="dnaB"
                     /locus_tag="PLR57_04260"
     CDS             <1..1087
                     /gene="dnaB"
                     /locus_tag="PLR57_04260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQQ40702.1"
                     /translation="LTDLVTFVPTAANVKNYIELVETKSVQRQLIRAGGEIIRDGMDD
                     EKELEDTLNAAERRIYDISMRKSQGSLIPIDQIVPEAYNLIGELAQRHGKITGVASGF
                     TELDRMTNGFQKSDLIIVASRPAMGKSSFAMNVAQYAALHDNRTAVVFSLEMSAEQLV
                     MRMLCTEASVDSQRIKEGLIGNSEMGQLMEVMDPMSRARIYIDDTSGATVPIIRSKCR
                     RLNARVGLDMIVIDYLQLMQSSGGRKSDNRMQEISDMTRQLKLLARELNVPIILLCQL
                     NRGPDQRADHRPIISDLRESGSIEQDADMVILLYRPAAYLDTPEYESGDNTSQIIIAK
                     HRHGSTGMVKLLWQGEYTRFRSIAHEE"
     gene            1119..1775
                     /locus_tag="PLR57_04265"
     CDS             1119..1775
                     /locus_tag="PLR57_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HQQ40703.1"
                     /translation="MKNQDMFETLSATLAASLFEETVYPWEVLPKIKEFVRALGPTLD
                     PAEYDHPDEDVWIHKSVKRYPNVYIEGLTIICAETEIRPGAFIRGSVLVGKSCVVGNS
                     TELKNCILFDHVQVPHYNYVGDSILGNYAHMGAGAICSNVKGDKTNVVVRGELAIETG
                     LRKFGAILGDHVEVGCNSVLNPGTVLEPYVSVYPLSNVRGVVKRGHIHKAHGEIVQRI
                     "
     gene            1979..>2438
                     /locus_tag="PLR57_04270"
     CDS             1979..>2438
                     /locus_tag="PLR57_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HQQ40704.1"
                     /translation="MIYFRHHSRLLTFRRPQGAAPCGSSVHIEAECGDPNARVQLRLY
                     HQNGSESFFDMQVSGGRATADLPMPEAPGLVWYYFIIRLQDGQTLYYGADSGEGALRQ
                     NEPRAYQITVYDGAFETPQSWREGIVYQIFPDRFRRSSWEDFRSRARYHVE"
BASE COUNT          554 a          696 c          705 g          483 t
ORIGIN      
        1 tctgaccgat ttggtcacgt ttgtgccgac cgcggcgaac gtgaagaact atatcgaact
       61 ggtcgaaacc aagagcgtgc agcgccagct catccgcgcg ggcggcgaga tcatccgcga
      121 cggcatggac gacgaaaaag agctggagga cacactcaac gccgccgagc ggcgcatcta
      181 cgatatctcc atgcgcaagt cgcaggggtc gctgattccc atcgaccaga tcgtgccgga
      241 ggcgtacaac ctcatcggag aactcgcgca gcgccacggc aagatcacgg gcgttgcgag
      301 cggctttacc gaactcgacc gcatgacaaa cggcttccaa aaaagcgatc tcatcatcgt
      361 ggccagccgt cccgccatgg gtaaatcgag ctttgccatg aacgttgccc agtacgccgc
      421 cctacatgac aaccgcacgg cggtcgtgtt cagcttggaa atgagcgccg aacagctcgt
      481 catgcgtatg ctctgcacgg aggcttcggt cgattcccag cgcatcaagg aaggcctcat
      541 tggcaactcc gagatggggc agctcatgga ggttatggat ccgatgagcc gcgccagaat
      601 ctatatcgac gatacgagcg gagcaacggt gccgattatc cgcagtaagt gccgccgcct
      661 caacgcgcgc gtcgggctgg atatgatcgt catcgactac ctccagctta tgcagtcttc
      721 cggcgggcgc aagagcgaca accgcatgca ggagatttcc gacatgacgc ggcagctaaa
      781 gctgctcgcg cgcgaactca acgtgccgat tatcctgctc tgccagctca accgcgggcc
      841 ggaccagcgc gccgatcacc ggccgatcat ctccgacctt cgcgagagcg gctccatcga
      901 gcaggacgcg gatatggtta tcctgctgta ccgtccggcc gcctacttgg atacgccgga
      961 gtacgaaagc ggtgacaaca ccagccagat catcattgcc aaacaccgcc acggttcgac
     1021 cggtatggtc aaactgctct ggcagggcga atacacgcgc ttccgctcga ttgcacacga
     1081 ggagtaaaaa cgccgagaaa cggagaaaca gcagcacaat gaagaatcag gatatgtttg
     1141 aaacgctttc cgcaacgctt gcggcaagtt tattcgaaga gacggtctat ccctgggaag
     1201 tcctgccgaa gatcaaggag tttgtgcggg cactggggcc gacgctcgac ccggcggaat
     1261 acgatcatcc cgatgaagac gtctggattc acaagagcgt taagcgctac cccaatgtat
     1321 atatcgaagg gctgaccatc atctgcgcgg aaacggaaat ccgccccggc gcgtttatcc
     1381 ggggaagcgt gctggttggc aaatcttgcg tggttggcaa ctcgacggag ctcaaaaact
     1441 gcattctgtt cgatcacgtg caggtgccgc actataacta cgttggcgac agcatcttag
     1501 gaaactatgc gcatatgggc gcgggggcga tctgctccaa cgttaagggc gacaagacga
     1561 acgtcgtcgt gcgcggcgaa ctggcgattg aaacagggct tcgtaagttt ggcgccatcc
     1621 ttggcgacca tgtggaagtt ggctgcaaca gcgttctcaa ccccggcacg gtgcttgaac
     1681 catacgtttc cgtttacccc ctctccaatg tacgcggcgt tgtcaagcgc ggtcatatcc
     1741 acaaagctca cggcgaaatt gtccaaagaa tttagttgtt gcccgcccgc gtatccggca
     1801 ggaaatcctg cggcgcgggc ggcttttttg cgcatttttc tgccgcaagg cgcggaattt
     1861 ttcgttgcga cgaattaaaa aagcatatct tgtttacgat ggcggaagaa atgtgaaaac
     1921 ctcgcaacat tccgcacaaa cttgtttttc gggggaagcc attcggtata ataagcgtat
     1981 gatttatttt cggcatcatt cgaggctgct cacctttcgc aggccgcagg gcgcggctcc
     2041 ctgcggcagc agcgtacata tagaagcgga atgcggcgat ccaaatgccc gcgttcagct
     2101 gcgtctttat caccagaacg gcagcgaatc gtttttcgat atgcaggttt ccggcggcag
     2161 ggcgacagcg gatttgccga tgcccgaagc gcccggtttg gtttggtact atttcatcat
     2221 tcgcctgcag gacgggcaaa cgctctatta cggagcggac agcggggaag gcgcgctccg
     2281 gcagaacgag ccgcgcgctt accagattac tgtttacgac ggcgcgtttg aaacgccgca
     2341 gagctggcgc gaggggatcg tctatcagat tttccccgac cggttccggc gcagcagctg
     2401 ggaggacttt cgctcccgcg cgcgatacca tgtggaaa
//
LOCUS       DAOMGY010000224         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_48038_length_2432_cov_3.534287, whole genome shotgun sequence.
ACCESSION   DAOMGY010000224 DAOMGY010000000
VERSION     DAOMGY010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48038_length_2432_cov_3.534287"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLR57_04275"
     CDS             <1..349
                     /locus_tag="PLR57_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HQQ40705.1"
                     /translation="QATAEYQKTIRAASVAKKEYDTLRVECEKERESRADELAKHDAK
                     LESLGKQVDQTLLARYEQVKLHHAVPIARVINSKCGGCNMSLPMVMLKKIATTDGIVE
                     CENCGRILYSGDQ"
     gene            372..712
                     /gene="rnpB"
                     /locus_tag="PLR57_04280"
     ncRNA           372..712
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLR57_04280"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            971..1979
                     /gene="trpS"
                     /locus_tag="PLR57_04285"
                     /pseudo
     CDS             971..1979
                     /gene="trpS"
                     /locus_tag="PLR57_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
     gene            1998..>2432
                     /locus_tag="PLR57_04290"
     CDS             1998..>2432
                     /locus_tag="PLR57_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40706.1"
                     /translation="MLRRFFIGVSEENMLSEQQFTSLRERALAFAHSSFTYLDFDAVE
                     HYAVLQDDQECVLLGGEKAETDVYELHWGANDPTPIIRVVKETGRETLVTFVPEEWKT
                     RFLASGFGEYGILREYWLEPLDKPLTPRIACEPLTTADATEAA"
BASE COUNT          609 a          658 c          726 g          439 t
ORIGIN      
        1 acaggcgaca gccgagtacc agaaaacaat ccgtgcggcg agcgtcgcca agaaagaata
       61 cgatacgctc cgcgtggagt gcgaaaaaga gcgcgaaagc cgcgcggacg agctggcaaa
      121 gcacgacgca aagctggaat ccctcggaaa gcaggttgac cagacgcttc tggccaggta
      181 cgagcaggta aagctgcacc acgccgtgcc gattgcgcgg gtcatcaaca gcaaatgcgg
      241 cggttgcaac atgtcgctgc cgatggtaat gctcaagaaa atcgcgacga cagacggcat
      301 cgttgaatgc gaaaactgcg gaaggatttt gtattcggga gaccagtaag aaaagcagca
      361 cctttacaac ttgagtaagg aaggcgaccg cgcctgccaa atggcgggtg aggaaagtcc
      421 gagcaccaca gggcggagtg ccgggtaaca cccggcggag gcgactccag ggaaagtgca
      481 acagaaagaa accgcccgcg gcaacgcggg taaggatgga aaggcgaggt aagagctcac
      541 ccgcgtcccg gcgacggaga cgcgctgtaa accccacttg gtgcaagatt ggtatgagag
      601 catttaatag ctgcccgcta cgctctcggg aatcgcatga gcctctcggc gacgattggc
      661 cccagataga tggtcgcact cgacagaact cggcttattc cttgctcaaa acttttttag
      721 aaacttacag cggctcaggg aacttggaaa actgagtacc ggctttgatt caggaagatg
      781 gtcacacccg acagaactcg gcttattcct tgctcaaaga tttttctata cgcgctcttt
      841 ggcacgctgc gaatgaacgg acgaacagcc ctgacgggtg gaacgtgcag aaacacaaac
      901 gcgccttgcg gcgcgttttg caaaagcaaa cgaaataacg gacactacgg gaaacgggat
      961 gggaacgaac atggaaacga ttacgacgga acaaaagaaa aaagcggtgc tctccggcgt
     1021 tcagccgacg agcgtattaa ctataggcaa ttatcttggc gcgctgaaga attgggtcgc
     1081 catgcaggac gagttcaacg ccttctacat ggttgccaac ctccacgcgc agaccgtccg
     1141 tcaggactct gcggaactgc gccgcaggag cctggaaacc ttcgcgctgt ttcttgcctg
     1201 cgggcttgac cccgagcgtt cgaccatctt cgtgcagtcg catgtgccgg agcactccat
     1261 gctcacctgg gcgctgagct gctgtacata catgggcgag ctttcccgca tgacgcagtt
     1321 caaggacaag agcgcgcgca atgccgacaa catcaacgcg ggtttgttta cctatcccgt
     1381 gctcatggcg gcggatatcc tgctctacca gtcgcattat atccccgtgg gcgcggatca
     1441 gaaacagcat gtcgagctca cacgcgatat tgcaatccgc ttcaaacacg tttggtgaaa
     1501 cgttcgtcgt gccggaaccg tatattccga aagtcggcgc gcggattatg agcctgcagg
     1561 agccggaaag caagatgagc aagtctgacc ccaacccgaa cgcttatatc cgcatcctcg
     1621 acgacccgga cacgattacg cgcaagctca aacgcgccgt aacggacagc gaaggcgaga
     1681 ttacagccaa cccggagcgc gcgggcgttt ataacctttt aaccatctac gccgcctgcc
     1741 gcggggaaac gattgagcag gcgcttgccg tgttcgaagg caaaggctac ggcgacctta
     1801 aggcgggtgt tgcggaagcg gtcatcgaac ttctccggcc gattcaggcg gagtatgcgc
     1861 gtgtgctcgc ggacaaggcg tacctgaacg gtcttatgaa gctaggcgcg gaacgcgcgc
     1921 agatctcggc gcgaaagacc gtcggcaagg tttaccacaa gatgggcttc gacgtataac
     1981 aaaccggcgg cggggaaatg ctccgccgct tttttattgg agtaagtgag gaaaacatgc
     2041 tgagcgaaca acaatttaca agcctgcgcg agcgcgcgct tgcgtttgcg cattcgtcgt
     2101 tcacgtatct ggatttcgat gcggtggaac actacgccgt gctgcaggac gatcaggaat
     2161 gcgtgctgct cggtggtgaa aaggcggaaa cggacgttta cgagctgcac tggggcgcaa
     2221 acgatccgac gccaatcatc cgcgtcgtaa aggaaacggg acgggaaacg ctggtcacgt
     2281 tcgtgccgga agagtggaaa acgcgatttc tcgcgagtgg ttttggcgag tacggcatcc
     2341 tgcgcgaata ctggctcgag ccgctggata aaccgcttac accgcgaatc gcctgtgagc
     2401 ctcttacgac cgcggatgcc acggaggcgg cg
//
LOCUS       DAOMGY010000225         2418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_48453_length_2418_cov_3.314431, whole genome shotgun sequence.
ACCESSION   DAOMGY010000225 DAOMGY010000000
VERSION     DAOMGY010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48453_length_2418_cov_3.314431"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /locus_tag="PLR57_04295"
     CDS             complement(<1..1653)
                     /locus_tag="PLR57_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQQ40707.1"
                     /translation="MSEQPKNGAVGAPFPIDDAALKATGQLRYVDDLKLPNMLYGKIL
                     FSSVPHARIKLLDTSAAEAMEGVRAVVSYKNTPAVYYNSSGETLDIFKTERVFDEIVR
                     YIGDRVAAVAADTPEIAAEAVRAIRVEYEPLPANFDPEAGMAEDAYPIHTHGDLLKGN
                     LMEIVKQSCGDAESAIASAPHVFEETFTVPPIHHGAIEPHGAVAFYDAGGKLTVWASS
                     QDSFAVRINLSRIFSLPMSRVRVSVPAMGGAFGGKVDMITEPVAAALSIQSGRPVKLI
                     YTRREDIVSSRTRHAMKIKLRMGFDSDGTILGEDMTVFCNAGAYASGTSNIVWAMCGK
                     FFKVHRCKNIRFTGYPVITNTPVGGPMRGFGSPQTFFAQQRLMQRAAAALGLDLYDMQ
                     VKNLTYPDGVDYRFDLPHGNAHPLDCLKRASELIGYEQCLAEQRASANERYRIGVGLA
                     VGAHGNGMFGIRTDITAMVLKMNDDGSCVLFSGSHEMGNASITMQKQMVSEVLGLPME
                     MIAAVSADTELTPYQLGDYSSRGAFVSGRAAMEVALDMRAKLC"
     gene            complement(1650..2132)
                     /locus_tag="PLR57_04300"
     CDS             complement(1650..2132)
                     /locus_tag="PLR57_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQQ40708.1"
                     /translation="MEVFRLTVNNRIFEVQARPNATLLSVLRDQLDLTGAKCGCGTGD
                     CGACTVLLDGEAVRSCIVPVKNAVGKQIITIEGLARGSELHPIQRAFVERGAIQCGFC
                     TPGMILAAKALLDHNPNPTEDEIRAALRFNLCRCTGYARIVEAVQFAAELLRKEAERA
                     "
     gene            complement(2151..>2418)
                     /locus_tag="PLR57_04305"
     CDS             complement(2151..>2418)
                     /locus_tag="PLR57_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HQQ40709.1"
                     /translation="GWAFEETDDFVQKALSGLRGAGVSASVTHYNFCTNGSHYAGEAG
                     IPTIGFGPSAETHAHTRDEWVALEQIQTALNGYLGLLGQFCPAQ"
BASE COUNT          454 a          720 c          761 g          483 t
ORIGIN      
        1 acatagtttc gcgcgcatat cgagcgctac ttccatcgcc gcgcggccgg agacgaacgc
       61 gccgcggctc gaatagtcgc cgagctgata gggggtcagt tccgtatccg ccgagacggc
      121 ggcgatcatc tccatcggga gtcccagcac ttcggacacc atctgctttt gcatcgtgat
      181 ggaggcgttg cccatctcgt gcgaaccgga gaagagcacg cagcttccgt cgtcgttcat
      241 cttgagcacc atggcggtga tatccgtgcg aatgccgaac atgccgttgc cgtgcgcgcc
      301 gaccgcgagg ccgacgccga tgcgataccg ttcgttcgcg cttgcgcgtt gttcggcaag
      361 gcactgctcg tagccgatca gctcggatgc gcgcttgagg cagtccaacg gatgcgcgtt
      421 gccatgcggc aggtcgaaac ggtagtcaac accgtccgga taagtcaggt ttttcacctg
      481 catgtcgtac aaatcgagcc cgagcgcggc agccgcgcgc tgcatgagcc gctgctgggc
      541 gaaaaatgtc tggggagagc cgaacccgcg catcgggccg ccgacggggg tgttcgttat
      601 gaccgggtag cccgtaaagc ggatgttttt gcagcggtgc accttaaaga acttgccgca
      661 catcgcccag acgatgttgc ttgtgccgga agcgtaagcg ccggcgttgc agaataccgt
      721 catgtcctcg ccaaggatcg tgccgtcgga atcaaagccc atgcgtagtt tgattttcat
      781 tgcgtgccgt gtgcgggagg agacgatatc ctccctccgg gtataaatca gcttgacggg
      841 acggccggac tgaatcgaaa gcgccgccgc gacaggctcc gtgatcatat ccaccttccc
      901 gccgaacgcg ccgcccatgg cgggcacgct aacgcgaacg cggctcatag gcagagagaa
      961 gatgcgcgaa agattgatgc gaacagcaaa actgtcctgc gaggaagccc aaaccgtgag
     1021 ctttccgcca gcgtcgtaaa aagcgacggc gccgtgcggt tcgattgcgc cgtgatggat
     1081 gggggggacg gtgaacgttt cctcaaagac gtgcggagcg gaagcgattg ccgattccgc
     1141 gtccccgcag ctctgcttga cgatctccat gaggttaccc ttgagtaaat cgccgtgcgt
     1201 atggattggg tacgcgtcct ccgccatacc cgcttcgggg tcaaagtttg cgggcagggg
     1261 ttcgtactcc acgcggatgg cgcggacggc ttcggcggca atctccggcg tatccgccgc
     1321 aacggcggcg acgcggtcac cgatatagcg cacgatctcg tcgaacacgc gctcggtctt
     1381 gaagatatcc agcgtttcgc cgctgctgtt gtaatagacg gcgggcgtgt ttttgtagga
     1441 gacgacggca cgcacgcctt ccatcgcttc ggcggcggac gtgtccagca gcttaatgcg
     1501 cgcgtgcggc acggaggaaa agagaatctt gccgtagagc atgttcggca gtttgagatc
     1561 gtccacgtac cggagctgac ctgtggcttt gagcgccgcg tcgtctatgg ggaaaggcgc
     1621 gccgaccgcg ccgtttttcg gctgttcgct catgcgcgtt ccgcctcctt tcgaagcaat
     1681 tctgcggcaa actgcaccgc ttccacgatg cgcgcgtaac ccgtacaacg gcagaggtta
     1741 aagcgaagcg ccgcgcggat ttcgtcttcc gtagggttcg ggttatggtc gagcagggct
     1801 ttcgcggcaa ggatcatgcc cggcgtgcag aagccgcact gaatcgcccc gcgctcgaca
     1861 aacgcgcgct ggatgggatg aagttcgctc ccgcgcgcga ggccttcgat ggtgataatc
     1921 tgttttccga ccgcgttttt gaccggaacg atgcaggagc ggacggcttc gccgtccagc
     1981 agcacggtgc aggctccgca gtcgcccgtt ccacagccgc atttggcgcc ggtaaggtcg
     2041 agctgatcgc gcagaacgct gagcagggtc gcgtttggcc ttgcctgcac ttcgaatatt
     2101 cggttgttga cggtaagccg gaatacttcc ataaaactcc tcaactttcg ctactgagcg
     2161 gggcagaact ggccgagcag accgaggtag ccgttcagcg cggtttgaat ttgctcaagc
     2221 gcaacccatt cgtcccgcgt gtgcgcgtgc gtttccgcag acggcccgaa cccgatggtc
     2281 gggatgcccg cttcgcccgc gtagtggctg ccgttggtgc agaagttgta atgcgtaacc
     2341 gacgcgctta cgccagctcc gcgaaggccg cttagcgcct tctgaacaaa atcgtcagtt
     2401 tcttcaaacg cccagccg
//
LOCUS       DAOMGY010000226         2412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_48651_length_2412_cov_2.837505, whole genome shotgun sequence.
ACCESSION   DAOMGY010000226 DAOMGY010000000
VERSION     DAOMGY010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48651_length_2412_cov_2.837505"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLR57_04310"
     CDS             <1..282
                     /locus_tag="PLR57_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HQQ40710.1"
                     /translation="NLVFLASTGVTLERGLTNTTYFEVEVKQTVARQNKNLVLMADHT
                     KFGHDALLTFCNFEDLSAVVTDQPLPPEYNAVIAQHGIRLLTPEASDLD"
     gene            complement(345..656)
                     /locus_tag="PLR57_04315"
     CDS             complement(345..656)
                     /locus_tag="PLR57_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40711.1"
                     /translation="MVRKTAVSALRIVFRESLSQLQSVRTAFTQRGMACLAFEPEAGS
                     AQSVIERLQPEIACWSGTMKLYIESTPDSVQIRVDNFYIDKPEEAEAVIRMGLILLEH
                     D"
     gene            complement(626..997)
                     /locus_tag="PLR57_04320"
     CDS             complement(626..997)
                     /locus_tag="PLR57_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40712.1"
                     /translation="MSAEWRILIASALIFIVIGVIYLIVDRRKHEKKEAFRRYWELNG
                     YDFGTFDEADYEGYSLKKDDWELHVCRSQERGSADWKLESIWRTRRHDPERKTFALQY
                     ARRACLLKICRRWFARPPFPR"
     gene            1332..>2412
                     /locus_tag="PLR57_04325"
     CDS             1332..>2412
                     /locus_tag="PLR57_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40713.1"
                     /translation="MKRSFIMLLALFACFALLGCAKAEEPAEPTASPTTTPTATPTPT
                     PEPTPEPEADYSVYAGEKLRAVDYLSNELESYRSQLTVYGDFAETENNYTQKALMYGT
                     NRNLVKDMNENWTENPYSGKSCIRCEIDTSGLDWGGWLFLNGYLPQGETVPKLNDGSR
                     AGEGLNLTGATALRFWARGETGSEIVEFFTAGFGYDGEWGTPLVDFPDTAKKQSLGFV
                     QLSTEWTEYTIPLRDLDLSSIVCGFGYVCSGTKTGDAANVFYLDDIRFVGRIDSLKTA
                     PVLLRSYETENIYIQNAAFSYDNALAAMAFLSEGMQDEAGQILDAFVYAVEHDRYEPG
                     RVRNAYAAGSIEAFPGWESGARLPGW"
BASE COUNT          540 a          738 c          636 g          498 t
ORIGIN      
        1 aacctggtgt tcctcgcttc cacgggcgtg actctcgagc gcggactgac caacacgacc
       61 tacttcgaag tcgaggttaa gcagacggtc gcgaggcaga acaagaatct cgtgctgatg
      121 gcggatcata ccaagttcgg ccacgacgcg cttttgacct tctgcaactt cgaagacctc
      181 agcgccgtgg tgacagatca gcctcttccc ccggagtaca acgcggtgat tgcgcagcac
      241 ggcatccggc ttttgacccc ggaagcgagc gatctggact gacaccccca tacgaatcac
      301 aagcccccga ctgaaacagt cgggggcttg tttgttgctt ccttctagtc gtgctccagc
      361 aggatcagcc ccatgcggat gactgcttcc gcttcttccg gcttgtcgat ataaaagttg
      421 tccacccgga tttgaacgga atccggcgtg ctctcaatat agagcttcat cgtgccgctc
      481 cagcaggcga tctccggctg aagccgttcg atgacgctct gcgcgctccc cgcctccggt
      541 tcaaacgcga ggcaggccat gccgcgctgc gtaaacgccg tgcgcacgct ttgcagctgc
      601 gacaggcttt cgcgaaagac aatcctcagc gcggaaacgg cggtcttgcg aaccatctcc
      661 ggcagatctt caaaaggcac gctcgacggg catattgcag cgcgaatgtt ttgcgctcgg
      721 gatcatgccg ccgggtacgc cagatggatt ccagtttcca gtccgcgctt ccccgttcct
      781 gcgacctgca aacgtgcagt tcccaatcgt cttttttcag tgaatagcct tcgtagtccg
      841 cctcgtcgaa cgttccgaaa tcatagccgt tcagctccca gtaccggcga aacgcttcct
      901 ttttttcatg ctttcgccgg tccacaatca aataaatcac gccgattacg ataaaaatca
      961 gtgcggacgc gatcagaatt ctccattccg cgctcatagc aacatctcct ttgcccgatt
     1021 tgccgccaac gcctgaccgg cagcttcatc ataccgaaaa cgcaagagga ttacaacgaa
     1081 tgtcagtccg gcgtgtattt cacagccgat tttttaaaat agaatcgaaa tttctttaat
     1141 ttcctccaaa agtgtgcaaa ctgcttaccg attgcacaaa tcacgcgcgt atactcgaat
     1201 cagaaaccgg aacttgattc agccgaatcc gcaaacaagg aggcgatgcc gccgtgcaga
     1261 agttcccacc gctctgcggc gcatgctgtc tttttacttc acacaacgct taacaaggag
     1321 gaattcacgc tatgaaacgc agttttatta tgcttctcgc cctgttcgcc tgcttcgcgt
     1381 tgctgggctg cgccaaggcg gaggaacccg cggagccaac ggcttctccc accacaacgc
     1441 ccaccgcaac ccctactcca acgccggaac ccacacccga gccggaagcg gattacagcg
     1501 tctacgccgg agagaagctc cgcgcggtcg attacttgag caacgaactg gaaagttacc
     1561 ggagtcagct gaccgtctac ggcgattttg ccgaaacgga gaacaactac acgcaaaaag
     1621 cgctcatgta cggcacgaac cggaatttag tcaaggacat gaacgaaaac tggacggaaa
     1681 acccgtattc cggcaagagc tgcatccgct gtgaaatcga caccagcggg ctggactggg
     1741 gcggctggtt gtttctgaac gggtatctgc cccagggcga aaccgtgccg aagctcaacg
     1801 acggctcccg cgcgggagag gggctaaatc tgaccggcgc caccgcgctt cgcttctggg
     1861 caagaggcga aacggggagc gaaatcgttg agttcttcac cgccgggttc ggttacgacg
     1921 gcgaatgggg cacaccgctc gtagacttcc ccgatactgc caaaaagcag agcctcggct
     1981 ttgtgcagct ctccacggag tggacggaat acaccatccc gctcagggat ttggatttga
     2041 gcagcattgt ctgcgggttt ggctatgtct gcagcggcac gaagacgggc gacgccgcca
     2101 acgtgtttta cctcgacgac attcgctttg tcggcagaat cgattcgctc aaaaccgctc
     2161 ccgtgctgct tcgttcctac gaaacggaaa atatctacat ccagaacgcg gcgttttcct
     2221 acgacaacgc gctcgccgcc atggcgttcc tctccgaagg aatgcaggat gaagcaggcc
     2281 agattctgga tgcgttcgtc tacgcggttg aacacgatcg ctatgagccc ggccgcgtcc
     2341 gaaacgccta cgccgcgggc agcatagagg ccttccccgg ctgggaaagc ggcgcgcgcc
     2401 tgcccggctg gt
//
LOCUS       DAOMGY010000227         2407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_48811_length_2407_cov_2.843963, whole genome shotgun sequence.
ACCESSION   DAOMGY010000227 DAOMGY010000000
VERSION     DAOMGY010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48811_length_2407_cov_2.843963"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..747
                     /locus_tag="PLR57_04330"
     CDS             82..747
                     /locus_tag="PLR57_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HQQ40714.1"
                     /translation="MSKLADRELPNYTRGEEIFNMVSHITGGALGVAALVLCVIKAAI
                     SGNAWAIVSSSIYGFTMVVLYCLSSVYHGLRPSLGKKVLQVLDHCSIYFMIAGTYTPI
                     VLVAMRPLYPFLAWIIFAFEWGITAIAVTLTAIDLKEYRAFSMLCYIVMGWGIVLATP
                     YATEALTQTGFTLLLAGGFAYTLGAVLYGIGANKRWFHCIFHIFVLIGSALQFFAIYL
                     FAI"
     gene            complement(879..1553)
                     /locus_tag="PLR57_04335"
     CDS             complement(879..1553)
                     /locus_tag="PLR57_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQQ40715.1"
                     /translation="MSIPYGFLKKTSLFRGTSEEELPTLLDCLSPTTREYAKNELILR
                     QGEDVSAVGLVLSGRAQVIEEDFWGNRSILADAWPGDLFAEAYAFLPGERLRVSVVAA
                     EPTSVMLLDAKRMLEVCSSACRFHTRLVQNILAESARKNLALTRKVSHMSKRSTREKL
                     LSYLSGQALGAGSDAFEIPFNRQQLADYLCVDRSAMSSELCKMRDEGLLVFDKNRFQL
                     KEEFSE"
     gene            1724..>2407
                     /locus_tag="PLR57_04340"
     CDS             1724..>2407
                     /locus_tag="PLR57_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQQ40716.1"
                     /translation="MIRKIIQIDEEKCNGCGACASACHEGAIAMVNGKAKLMRDDYCD
                     GLGNCLPACPTGAITFVEREAAAYDEEAVKAHLETAKPKHAAPATLACGCPGTHSRVI
                     ERHDEPQAEEKSEGCACGGHDAPAPSRLSQWPVQIKLVPVNAPYFNGAKLLVAADCTA
                     FARGDFHERFIKNHVTLIGCPKLDMTDYSEKLTEILRNNNIKSVTVVRMEVPCCGGIE
                     AAVKTALVNS"
BASE COUNT          528 a          717 c          659 g          503 t
ORIGIN      
        1 ccaaagtgtt gttgccccgg cctgcgcaat gcggtatact tggggtcaat tacgggtcaa
       61 accacgaaga ggagcgcaca agtgagcaaa ctggccgacc gcgaactgcc caactatacc
      121 agaggcgaag aaatcttcaa tatggtgtcc catatcacgg gcggtgcgct tggcgttgcg
      181 gcgctcgtgc tctgcgtaat caaggccgcc atctccggca acgcctgggc aatcgtttcg
      241 tcgtcgattt acggctttac gatggtggtg ctgtactgcc tttccagtgt gtaccacgga
      301 ctgcggcctt cgctgggaaa gaaagtcctg caggtgctcg accactgctc gatctacttt
      361 atgatcgcgg gaacctacac gcccatcgtg ctcgttgcca tgcgcccgct ctatccgttc
      421 cttgcgtgga ttatctttgc cttcgagtgg ggcatcacag ccatagccgt cacccttacc
      481 gccatcgacc tcaaggaata ccgcgcattc tccatgctct gctatatcgt gatgggctgg
      541 ggcatcgtgc tcgcaacgcc gtacgcaacg gaagcgctca cgcagaccgg ttttacactg
      601 ctgcttgcgg gcgggtttgc ctatacactc ggcgcggtgc tctacggcat aggcgcaaac
      661 aagcgctggt tccactgcat ttttcacatt ttcgtgttga tcggcagcgc gctccagttc
      721 ttcgccatct acctgttcgc aatataacga agggggcgtt cttcttttct gaagaaaaga
      781 agcaaaagac tactataaca atagaacttc tcaaacaata atgagaagtt ctttttgatt
      841 ctttttcttt caagaaaaag aatggagaag ccttcgtttt attccgagaa ctcttctttg
      901 agctgaaagc ggttcttgtc gaacacaagc agcccttcgt cccgcatctt gcagagctcg
      961 ctgctcatcg cgctgcggtc cacgcagaga tagtccgcga gttgctggcg gttgaacgga
     1021 atttcgaacg cgtcgcttcc cgcgccgagt gcctgaccgg agaggtagga aagcagtttc
     1081 tcgcgcgtgc tgcgcttgga catatgcgaa acctttcgcg tgagcgcgag atttttgcgc
     1141 gcagactcgg caaggatgtt ctgcaccagc cgggtatgga agcggcaggc gctggagcag
     1201 acctccagca tacgttttgc gtcgagcagc atcacgctgg tcggctccgc ggcaacgacg
     1261 ctcacgcgca gccgttctcc cggcagaaac gcatacgcct ccgcaaagag atcccccggc
     1321 caggcgtcag caaggatgct gcggttgccc cagaaatctt cctcaatcac ctgcgcgcgc
     1381 ccggaaagca ccagccccac ggcggaaacg tcctcgccct gacgcaggat cagctcgttt
     1441 tttgcgtatt cccgcgtggt tggcgagagg cagtccagca acgtcggcag ctcttcttcc
     1501 gaggtgccgc gaaacaggga tgttttctta agaaatccgt atggtatgct catatcgtgc
     1561 ctccgctcct gcgataccaa tattctacaa tgattttttt cgcattgacg ttgtttttac
     1621 aacggacttg tttgattcat tttagtatag taagctcgta aagtcaaaca acacgcgacg
     1681 cccgccagcc ggacgacgaa cgaaccgaaa ggaagaaacc gacatgatcc gcaaaattat
     1741 tcagatcgac gaagaaaaat gcaacggctg cggcgcctgc gcttccgcct gtcacgaagg
     1801 agccatcgcc atggtcaacg gaaaagcgaa gctcatgcgg gacgactact gcgacggact
     1861 cggcaactgc ctgcccgcct gcccgacggg cgcgataacg ttcgtggagc gggaagccgc
     1921 agcttacgac gaagaggcgg tcaaagcgca cctcgaaacc gccaagccga agcatgccgc
     1981 accagcaacg cttgcctgcg gctgcccggg cacgcattcg cgcgtcatcg agcggcacga
     2041 cgagccgcaa gcagaggaaa agtccgaagg ctgtgcctgc ggcgggcacg acgcgcccgc
     2101 gccttcccgc ctgagccagt ggccggtgca gatcaagctc gttccagtca acgcgccgta
     2161 cttcaacgga gcgaaactgc tcgttgccgc cgactgcacg gcgtttgccc gcggcgactt
     2221 ccacgaacgg ttcatcaaga accacgtaac gctgatcggc tgtcccaagc tggacatgac
     2281 ggactactcc gaaaagctca cggaaatcct gcgcaacaac aacatcaaga gcgttacggt
     2341 ggttcgcatg gaggttccct gctgcggcgg catcgaagct gcggtcaaga ccgcgctcgt
     2401 caacagc
//
LOCUS       DAOMGY010000228         2401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_48983_length_2401_cov_2.054987, whole genome shotgun sequence.
ACCESSION   DAOMGY010000228 DAOMGY010000000
VERSION     DAOMGY010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48983_length_2401_cov_2.054987"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLR57_04345"
     CDS             <1..337
                     /locus_tag="PLR57_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40717.1"
                     /translation="SLIDSSNVIILFGVSIGSTDKIWWKKIGDWLITDPDHQLVIFVY
                     LPKVDRENSGQHLVSIEETQDQFLINTDISPQEHASLRQRIQVIVNYPLFGSCMCVTK
                     QTRDKTMAN"
     gene            complement(391..1641)
                     /gene="glyA"
                     /locus_tag="PLR57_04350"
     CDS             complement(391..1641)
                     /gene="glyA"
                     /locus_tag="PLR57_04350"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQQ40718.1"
                     /translation="MKFTPTHPIVAQVDPEIARAMELELGRQRDHLELIASENFVSPA
                     VLAAMGSPLTNKYAEGYPGKRYYGGCEYVDIVETLACERAKALFHADHANVQPHAGAQ
                     ANLAVYYALLNPGDTVLAMNLNHGGHLTHGHTANASGKLYHFVQYGVREDTGYIDYDE
                     LERLAHEHKPKLIVAGASAYSRIIDFERIGAVAKAVGAYFMVDMAHISGLIAAGEHPN
                     MFPYADVATSTTHKTLRGARGGLILCKEELAAKIDKAVFPGMQGGPLMHVIAAKAVCF
                     FEAMQPEFKTYQHQIVLNAKALCETLQQNGLRIFSGGTDNHLMLVDLRELGITGLSVQ
                     NLLDRANITANRNAIPFDTRNKLETSGMRFGTPAITTRGMKEPEMRQIGNMIARIIKE
                     GESAVPEVKQEVLALCKRFPLYEE"
     gene            complement(1804..2235)
                     /locus_tag="PLR57_04355"
     CDS             complement(1804..2235)
                     /locus_tag="PLR57_04355"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HQQ40719.1"
                     /translation="MEEQSCSIGLVTEVHGSLAKIRFLRSAQCAHCGACLTAGESEME
                     VELLNPVGAKVGDHVSVNLEPKRVVQASLLAYALPLALLLLGVWGGSRISELYALLFG
                     VAGFGIGYLILRLYEKKGKKETFRPRIVAVLNRQTDLPEEE"
     gene            complement(2243..2401)
                     /locus_tag="PLR57_04360"
                     /pseudo
     CDS             complement(2243..2401)
                     /locus_tag="PLR57_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569012.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          500 a          665 c          648 g          588 t
ORIGIN      
        1 ctcacttatt gattcaagca atgtaattat attattcggt gtttcaattg gttcaacaga
       61 taaaatatgg tggaaaaaaa taggcgactg gttaataaca gatccagatc accagcttgt
      121 tattttcgta tatctaccaa aagtagatcg agaaaattcc ggccagcacc ttgtttcaat
      181 agaagaaaca caagatcaat ttttaataaa tacagatatt tctcctcaag agcatgcttc
      241 gttacgtcag cgaattcaag taatcgtgaa ctatcctctg tttggcagtt gcatgtgcgt
      301 tactaagcag acacgagata aaactatggc taattaatag attcaaattc agaagcgccc
      361 tcgcaggcgc ttcttttcat tcactctcgg ttattcctca tagagcggga accgcttgca
      421 gagcgcaagc acttcctgct tgacttccgg cacggcggat tcgccttcct tgatgatgcg
      481 tgcgatcata ttgccgatct gccgcatttc aggctccttc atgccgcgcg tagtgattgc
      541 gggcgtgccg aatcgcatgc cgctggtctc cagtttgtta cgcgtgtcaa atggaatggc
      601 gttgcggttg gctgtgatgt tcgccctgtc cagcagattc tgcacgctca aacccgtgat
      661 gccaagttcg cgcagatcga ccagcatcag gtggttgtcc gttccgccgg agaagatgcg
      721 caggccgttt tgctggagcg tttcgcagag cgctttggcg ttgagtacga tctggtgctg
      781 atacgtctta aattccggct gcatcgcttc gaagaagcag acggcttttg cggcgatcac
      841 atgcatcagc gggccgccct gcataccggg gaataccgct ttatcgatct ttgccgcgag
      901 ttcctcctta cagagaatca acccgccgcg cgcgccgcgc agggtcttgt gggtagtcga
      961 ggtggcgacg tccgcatacg ggaacatatt cgggtgttcg cccgccgcga tcaggccgga
     1021 gatatgcgcc atatccacca tgaaatacgc gccgactgcc tttgcaaccg ctccgatacg
     1081 ctcaaaatca atgatgcgcg agtacgcgct tgcgccagca acaatgagct tgggcttgtg
     1141 ctcgtgcgcc aatctctcca gctcatcgta gtcgatatag cccgtgtcct cgcgtacgcc
     1201 gtactgcaca aaatgataga gtttgccgga agcgttggcc gtgtgcccgt gcgtcaggtg
     1261 tccgccgtgg ttgaggttca tggccagcac agtgtcgccg gggttgagca gcgcgtaata
     1321 gacggcaagg tttgcctgcg cgccggcgtg cggctgaacg ttggcgtggt cggcatggaa
     1381 cagcgctttt gcgcgttcgc aggcgagcgt ttcaacgatg tctacgtatt cacaaccgcc
     1441 gtagtaacgt ttgccgggat acccttcggc gtacttgttg gtcagcgggc tgcccatagc
     1501 ggcgagcacc gcgggagaaa caaagttctc cgaagcgatg agctccagat gatcgcgctg
     1561 gcggccaagc tcgagttcca tggcgcgtgc gatttccggg tctacctgag ctacgattgg
     1621 atgcgtcggc gtaaatttca tttttctttt tcctatccct ttattttaat aacattgcgc
     1681 tgttatgagc aatcctatat gatatacagt ccgcttccgg attgcgcttc gcccgcgcga
     1741 tccgcataca cagagggcgc gggcgatgtt gaatacccta ttcggttgtc cgcccgaccg
     1801 cgctcattct tcttccggca ggtccgtttg ccggttcagg acggcgacaa tgcgcgggcg
     1861 gaaggtctct ttcttccctt tcttttcata gagccggagg atcaggtacc cgattccgaa
     1921 tcctgccact ccaaagagaa gcgcatagag ttccgaaatc ctgctgccgc cccaaacgcc
     1981 gagaagcaga agcgccagcg gcagcgcgta cgccagcagg cttgcctgca ccacgcgctt
     2041 tggctccagg ttgacgctga catggtcgcc aaccttcgcg ccgacggggt tgagaagctc
     2101 gacttccatt tcgctctccc ccgccgtcag acaggcgccg cagtgggcgc actgcgcgct
     2161 gcgcaggaag cgaattttag caagcgagcc gtgaacttcc gtcacgagac ctatactgca
     2221 gctttgctct tccaacgatt cctcactctt ccttttcggt gcaggcgata tgcagttcta
     2281 cgagctgctt gtcgtccaca aagtccggcg ctccggtcat cgggcaggaa gcgttctaca
     2341 ccttcgggaa ggcgataaca tcccttatgc tcgaagagcc tgttatgatc atggcgaggc
     2401 g
//
LOCUS       DAOMGY010000229         2400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_48999_length_2400_cov_3.888699, whole genome shotgun sequence.
ACCESSION   DAOMGY010000229 DAOMGY010000000
VERSION     DAOMGY010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48999_length_2400_cov_3.888699"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="PLR57_04365"
     CDS             complement(<1..1590)
                     /locus_tag="PLR57_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQQ40720.1"
                     /translation="MRFTSEQQEAIQAEGNVIVSAAAGAGKTAVLTERVVSRVALGSP
                     IDSMLVLTFTRAAASEMKTRITNRLHKLADESVDQKQARYLRRQAKNVDSAYISTIHA
                     FCARVLRRHYHAVGLPAQSRTADEMESAALMETVRDELLTELAASEDADYHTLLACFG
                     GEQQAWDAVMQTFRFTRAEADPAAWLDENSARYQSEIALVRLLDDAVDYCKHEVRLMM
                     EPILRAKDSLSPDWASVISVLDGDLSRYRALALCKSYDEYREALNSIEYDTMRFPKGT
                     SEEEKDPIKDARQLGKDLIKAQKERFLRSRVEALSALKRSGEAIAALSSVVKKLEAAY
                     TAAKRASALQDYDDLEHLTLEALKQEPIAREYRERFHWIAVDEYQDSNRVQEAILRRI
                     SRGNNLFFVGDVKQSIYRFRQAEPGLFLEKLASFSGGAGSRVDLTRNFRSSREVLSCV
                     NETFSAILSTEAGDIEYDSRARLACGGDPPPGGAELHLIRKTRAETEGEEDALEDAAD
                     MEVEARLIADRIRRMMESETYI"
     gene            complement(1574..>2400)
                     /locus_tag="PLR57_04370"
     CDS             complement(1574..>2400)
                     /locus_tag="PLR57_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQQ40721.1"
                     /translation="RQKATLFLLVETVRQSALAVTRQLRAGSFEPAATEARFGVGQPF
                     PPIRLALADGREALVGGKIDRIDRVRVSGGECLRIIDYKTGGKDFDFSGILQGLTLQL
                     PLYLAAASAGGQVRAGMYYMPVTQPAVSDSEEDIEAAVADAFRLQGLTVSNLEVLHAS
                     ENNMSGQSGVLYKVERTGEDAYSGSVCTAGELEALLTLARKKSEETLARMLSGEMTAS
                     PAARKKNRPACEYCEYNSVCRFDPAAPGCSVRLYKTVRQADFFKLIAGGDADALYE"
BASE COUNT          432 a          779 c          694 g          495 t
ORIGIN      
        1 gatataggtt tcgctctcca tcatccggcg gatgcggtcg gcaatcagcc gcgcttcgac
       61 ctccatatcc gccgcgtctt ccagcgcgtc ctcttcccct tccgtctccg cgcgggtttt
      121 gcggattaag tgcagttccg cgccgcccgg cggtgggtcg ccgccgcagg ccagacgcgc
      181 gcgggaatcg tactcaatat cccccgcttc ggtggaaagg atggcggaaa aggtttcgtt
      241 gacgcaggaa agcacttccc gagaactgcg gaagttccgc gttaaatcca cgcggctgcc
      301 cgcgccgccg gaaaaggacg cgagtttttc caaaaacaat cccggctccg cctgccggaa
      361 gcggtagatg gactgcttga catcgcccac gaaaaacagg ttgttcccgc gtgaaattcg
      421 cctcaaaatc gcttcctgca cgcggttgct gtcctgatat tcgtccaccg cgatccagtg
      481 gaaccgctct cggtattccc gggcaatcgg ctcctgtttg agcgcttcca gcgtcaggtg
      541 ttcaagatcg tcatagtcct gcaaggcgga ggcgcgcttg gcggcggtat aggccgcttc
      601 cagctttttg accacgctcg aaagcgcggc aatcgcttcg ccggagcgtt tgagcgcgga
      661 caacgcttcc acgcgggagc gcaaaaaccg ctccttctgc gccttgatta agtccttgcc
      721 cagctgacgc gcgtccttaa tcggatcttt ttcctcttcg gacgtgcctt tgggaaagcg
      781 catggtgtcg tattcaatgc tgttcagcgc ttcgcggtac tcgtcgtagc tcttgcagag
      841 cgccagcgcg cggtaacgcg aaaggtcgcc gtccagcacg gagatgacgc tcgcccagtc
      901 cggcgagaga gaatctttcg cgcgcagaat cggttccatc atgaggcgca cttcgtgttt
      961 gcagtagtcc accgcgtcgt ccagcaagcg gacaagcgcg atttcgctct ggtagcgcgc
     1021 gctgttttca tcgagccagg ctgcggggtc ggcttccgcg cgcgtaaagc ggaaggtctg
     1081 catcaccgcg tcccaggcct gctgttcgcc gccaaagcag gccagcagcg tgtgataatc
     1141 cgcatcctcg ctggctgcaa gttcggtcag cagttcgtcc cgcacggtct ccattaaggc
     1201 cgcgctctcc atttcgtccg cagttcggct ttgggcgggc aagcctaccg cgtggtaatg
     1261 ccgccgcagt acgcgcgcgc agaacgcgtg gattgttgaa atatacgcgc tgtctacatt
     1321 tttcgcctga cggcgcagat agcgagcctg tttctgatcg actgattcgt ccgcaagctt
     1381 atggagtcgg tttgtgatac gggttttcat ttcggaagcc gccgcgcgcg taaacgtcag
     1441 caccagcatg ctgtcgatgg gcgaacccag cgccacgcgg gagactacgc gttcggtcag
     1501 aaccgctgtc ttacccgcgc cggccgcggc ggaaacgatg acgttgcctt cggcctggat
     1561 cgcttcctgt tgctcactcg taaagcgcat ccgcgtcacc tcccgcaatc agcttgaaga
     1621 agtccgcctg tctgacggtt ttatacagcc gcaccgaaca gcccggcgcg gccgggtcaa
     1681 agcggcaaac gctgttgtac tcgcagtact cgcaggccgg tcggtttttc tttcgcgccg
     1741 cgggcgaggc agtcatttcc ccgctcagca ttcgagccag cgtctcttcg gacttttttc
     1801 gcgcaagcgt caaaagcgct tccagctcgc ccgccgtaca gacgctgccg gaatacgcgt
     1861 cttcgcccgt ccgctccacc ttgtaaagaa cgccggactg cccgctcata ttgttttcac
     1921 tggcatgaag cacttcgaga ttcgaaaccg tcagcccctg caggcgaaac gcgtccgcga
     1981 ccgccgcctc gatatcctcc tcggaatcgg aaaccgcagg ctgggtcacc ggcatgtagt
     2041 acatgcccgc gcgcacctgc ccgccagcgc ttgcagccgc aagatacaac ggaagctgga
     2101 gcgtcaaccc ctgcagaatg ccggagaagt caaagtcctt gccgccggtc ttgtagtcga
     2161 taatgcgcag gcactccccg ccggagaccc gcacgcggtc gatgcggtcg atctttccgc
     2221 caacgagcgc ttccctgccg tccgccagcg caaggcgaat cggcggaaac ggctgaccaa
     2281 cgccaaaccg cgcttccgtt gcggcgggtt caaagctgcc cgcgcggagc tggcgtgtaa
     2341 ccgcaagcgc gctctgcctg accgtttcga cgagcaaaaa cagcgtcgct ttctggcgtt
//
LOCUS       DAOMGY010000230         2396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_49132_length_2396_cov_2.951730, whole genome shotgun sequence.
ACCESSION   DAOMGY010000230 DAOMGY010000000
VERSION     DAOMGY010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49132_length_2396_cov_2.951730"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /gene="proS"
                     /locus_tag="PLR57_04375"
     CDS             complement(<1..1247)
                     /gene="proS"
                     /locus_tag="PLR57_04375"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQQ40722.1"
                     /translation="MRMSELLGERYKEKPADCTVESHALMVRGGYIKQVNTGVYTLLP
                     PGKRITTKIERIIREEMDRIGGQEVLFPVVLPADLWKESGRFTSVGKELVRLTDRWGA
                     DMVLGMTHEEAAVHLARDAAKSYAKYPFMIYQIQTKFRDEPRARGGLIRVREFTMKDA
                     YSFHTSQADLEAYYARCYEAYERIFKRAGCKNVIAVLSDSGMMGGKVSHEFMLLTAIG
                     EDTLVICDSCDFKANLEAAPSVVDNSAFGVEESLTLVPTPEMATIEEVAGFLHKPHTS
                     ACKAVVYQKNADDSYVIAFVRGDLEVHETKLRNAIGAEIHPGVITPESGIVAGFIGPM
                     GLDAKKITVVFDRSLEGIGALVCGANKADHHYTGFNIARDYGKVTYLDIAKATPGGIC
                     PVCGKKTLRVSNGIEVGNIFQLGT"
     misc_binding    1459..1697
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1779..>2396
                     /gene="cysE"
                     /locus_tag="PLR57_04380"
     CDS             1779..>2396
                     /gene="cysE"
                     /locus_tag="PLR57_04380"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HQQ40723.1"
                     /translation="MFRTLKSDIQAVLHRDPAARSALEVMLCYPGFRAVRMHRRANFF
                     YTRGMKLLGRMISESCRFRTGIDIHPAATIGKRLFIDHGVGVVIGETAVIGDDVTIYQ
                     GATLGGTGKDVGKRHPTLGDHVTVSAGAAVLGPVTVGSHAKIGAGAVVLKDVPPFSTV
                     VGVPGHVVRLYGCKVPCDKVGQCDDGNCDCIGTELCVRTRENLGGE"
BASE COUNT          510 a          680 c          708 g          498 t
ORIGIN      
        1 gtgccgagct ggaagatatt gcccacctcg atgccgttgc tcacgcgcag cgtctttttg
       61 ccgcagacgg ggcagatgcc gccgggggtc gcttttgcga tatccagata ggtcaccttg
      121 ccgtaatcgc gggcaatgtt gaagccggta tagtggtgat ccgccttgtt cgcgccgcag
      181 accagcgcgc caatgccttc cagcgagcgg tcgaacacga cggtgatctt cttggcgtcg
      241 agccccatag gcccgatgaa tccggctacg attccgcttt ccggcgtaat tacgcccgga
      301 tggatctccg cgccgatggc gttgcggagc ttggtttcgt gcacttccag atcgccgcgc
      361 acaaaagcga tgacgtagga atcatctgcg ttcttctggt agacaaccgc tttgcaggca
      421 gaggtatgcg gcttgtgcaa aaagcccgcg acttcctcaa tggtcgccat ttccggcgtg
      481 ggaaccagcg taagcgattc ctccacgccg aaagcggagt tgtccacgac gctcggtgcc
      541 gcttccagat tggccttaaa gtcgcagctg tcgcagatga ccagcgtatc ctcgccgatg
      601 gcggtgagca gcatgaattc gtgggaaact tttccgccca tcatgccgga atcggacaga
      661 acggcgatga cgtttttgca gcccgcgcgt ttaaagatgc gctcataggc ctcgtagcac
      721 cgcgcataat acgcttccag atccgcctga gaggtatgga aggagtatgc gtccttcatg
      781 gtaaactcgc gaacgcggat cagtccgccg cgcgcgcggg gttcgtcgcg aaacttcgtt
      841 tgaatctggt aaatcatgaa cgggtactta gcgtaggact ttgccgcgtc gcgcgcaagg
      901 tgaacggcgg cttcctcatg cgtcatgccg agcaccatat ccgcgcccca gcggtcggtc
      961 aggcggacaa gctccttacc gacgctcgta aaacgaccgg attccttcca aaggtcggcg
     1021 ggcagcacaa cggggaagag cacttcctgc ccgccgatgc ggtccatctc ctcgcggatg
     1081 atgcgttcaa tttttgtcgt aatgcgcttt cccggaggca gcagcgtgta aacgccggtg
     1141 ttgacctgct taatatatcc gccgcgcacc atcagcgcgt ggctttcaac ggtgcagtcg
     1201 gcgggcttct ccttatagcg ttcgccgagc agttcgctca ttctcatagt tcttcacctc
     1261 atacaaatca ataaacacct tattatcgcc tagattgcga aatttgacaa gggcggattc
     1321 cgctttcaaa cacggttttt taccaaaatc cggcaagagc gggcgttgaa ggcggcggaa
     1381 gcggaaatgt taaattgtgg aaaacacgct tgacaaaact ttcagccata acatacactg
     1441 tattgagaat aaaagaggaa aaaggcgcag atcggaaaag agtacgcgcg ttcactcgcc
     1501 ccaagagagg attggtcacc ggctgaaagc cgatccgggc aaaacgagcg gaagtgcctt
     1561 ccgagagccg ctgccgacaa caaacgctta tgcaaaagtg tttcagtacc ggcggacgat
     1621 tcagccgcgt taaggctttg agcgggggag gattctcccc aaataaagtg gaaccacgga
     1681 gcgcattcgt ctttataacg ggtgcgcctt tttgtatgtt tacccgcgtg aacgcggaga
     1741 cacaaacgac gatttaaaat gataaggaag aaacggatat gtttcgaacg ttaaaaagcg
     1801 atattcaggc ggtgcttcac cgcgaccctg ctgcgcgctc cgcgctggag gtcatgctct
     1861 gctatccagg cttccgcgcg gtacggatgc accgcagggc gaactttttc tatacgcgcg
     1921 gcatgaagct gctgggcagg atgatttccg aatcgtgccg cttccgcacg ggcatcgata
     1981 tccatccggc ggcaaccatc ggcaagcgtc tgttcatcga ccacggcgtt ggcgtagtca
     2041 tcggcgaaac cgccgtgatc ggagacgacg tgaccatcta tcagggcgcg accctcggcg
     2101 gtaccggcaa ggacgttggc aagcgccacc cgaccctcgg cgaccatgtt accgtttccg
     2161 cgggcgcggc ggtgctaggc cccgtaacgg tgggcagtca cgcaaagatt ggcgcgggcg
     2221 cggtcgtgct caaggacgtt ccgccgtttt caacggtggt cggcgtgccg gggcatgtgg
     2281 tgcgcctcta tggctgtaag gtgccctgcg acaaggttgg ccagtgcgac gacggcaact
     2341 gcgactgcat cggcaccgag ctctgcgtgc gcacgcgcga gaacctcggc ggcgag
//
LOCUS       DAOMGY010000231         2386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_49448_length_2386_cov_3.700558, whole genome shotgun sequence.
ACCESSION   DAOMGY010000231 DAOMGY010000000
VERSION     DAOMGY010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49448_length_2386_cov_3.700558"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..746
                     /gene="rdgB"
                     /locus_tag="PLR57_04385"
     CDS             153..746
                     /gene="rdgB"
                     /locus_tag="PLR57_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HQQ40724.1"
                     /translation="MKLILATNNAHKMREIYEILGGDFPEMQTLSQAGLSIDVVEDGD
                     TFEANAVKKAEEVLQKSGYEAALADDSGLMVDYLGGAPGVYSARYAGEGHNDADNNLK
                     LMADMEGVPTEQRACHFVSAVALARKGKPTICVRGTVDGVLLTEAHGQNGFGYDPYFF
                     YPPLNRAFAELSAEEKNSVSHRKRALEALKRELKKEA"
     gene            743..1210
                     /locus_tag="PLR57_04390"
     CDS             743..1210
                     /locus_tag="PLR57_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQQ40725.1"
                     /translation="MKRIAVFADTHNKFNRLPEAIEKLGKVDMLFHLGDFGSDAEIIA
                     DKLGVPAFAVRGNNDYASVLPRLRAELVEDAWILLVHGDAYYTTGQLFDEAEKHNCLA
                     VLFGHTHEPLLTASGKVLLLNPGSLSLPRHGYAPSFAVLEVEGKDINAKIITL"
     gene            complement(1252..1809)
                     /gene="scpB"
                     /locus_tag="PLR57_04395"
     CDS             complement(1252..1809)
                     /gene="scpB"
                     /locus_tag="PLR57_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQQ40726.1"
                     /translation="MEDRTLINTIECMLFVAGDPVPVSEIARVLDCSLAVAKGALSEM
                     EQLYKSEGRGIQLLVTADTAQLVSNRDYIDAVKKLLNPDETKGVSQSLLETLAVIAYR
                     QPVTRADVEKVRGVRCDYAVTQLQKLNLIVEVGRKDTVGHPSLFGTTDQFLRQFGIHS
                     LDEMPEYLRFSQEIPEAPGEEIPVV"
     gene            complement(1866..>2386)
                     /locus_tag="PLR57_04400"
     CDS             complement(1866..>2386)
                     /locus_tag="PLR57_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HQQ40727.1"
                     /translation="AEEDPEELLIRQLREYKAFKEATEQLRQLFEAAQDGKTRLPEDV
                     PLPPKEVTLDGASMDGLFSAFLVMLERKKDEPERIGPQQVRPDRYTVRTQTGKIRTIL
                     QQKAAVFFEELFESNTTRMEMIVTFMALLEMIARGEILLKQSAPFAPIRISADKLLLD
                     DETYEYMDESED"
BASE COUNT          524 a          648 c          678 g          536 t
ORIGIN      
        1 acgttctaat gatttatcaa aattacatga cttttttaaa gttcaaaaaa cggagctttt
       61 gaacgctcga gagcaaattc ctttacggcg cgggcattct ctgttagaat agccacagga
      121 ttttaaccaa ttgtgagcaa ggatcatcaa gcatgaaatt aatcttagca accaacaacg
      181 cgcataaaat gcgggaaatt tacgaaattc tgggcggcga ttttcccgaa atgcagacgc
      241 tttcgcaggc ggggctttcc atcgacgtgg tggaagatgg agataccttc gaagccaacg
      301 ccgtcaagaa ggcggaagaa gtgctgcaaa aatccggtta tgaagcggcg cttgcggacg
      361 acagcggcct gatggtggac tacctcggcg gcgcgcccgg cgtgtacagc gcgcggtacg
      421 cgggggaagg gcataacgac gcagacaata acttaaagct catggcggat atggaaggcg
      481 ttccaaccga gcagcgcgcc tgccatttcg tttccgccgt cgcgctggcg agaaaaggca
      541 agccgacgat ctgtgtgcgg ggcacggtgg acggcgtact gctgaccgaa gcgcatggac
      601 agaacgggtt cgggtacgat ccatacttct tctacccgcc gttgaaccgc gcctttgcgg
      661 aactctccgc tgaggaaaag aattcggtca gccaccgcaa acgcgcgctg gaagcgctga
      721 agcgggagtt gaaaaaagaa gcatgaaacg catcgcggtc tttgccgata cccataataa
      781 attcaaccgc ctgccggaag cgattgagaa actgggcaaa gtggatatgc tgttccattt
      841 gggcgatttc ggctcggacg cggagattat tgcggacaaa cttggcgttc cggcttttgc
      901 cgtgcgcggc aacaacgatt acgcttccgt tttgccgcgc ctccgtgcag aactcgtgga
      961 ggacgcatgg atcctgctcg tacacggcga tgcgtattat acgacagggc agctctttga
     1021 cgaagcggaa aagcacaact gtcttgctgt gcttttcggg catacgcacg agccgctgct
     1081 cacggcgagc ggaaaggtgc ttttgctcaa cccgggcagt ctttcgctgc ctaggcacgg
     1141 ctacgcgccg tcgtttgcgg tgctggaggt ggaaggcaag gatatcaacg caaagatcat
     1201 cacgctataa aatgagccgc tctgacgagc ggctcttgtt tttaataatc ttcaaacaac
     1261 ggggatttct tcgccgggcg cttccggtat ctcctgcgag aaacgcaggt actccggcat
     1321 ttcgtccagc gaatgaatgc cgaactgccg caaaaactga tcggtcgtgc caaagaggga
     1381 agggtggccg acggtatcct tacgcccgac ttccacgatc agattgagct tttgcagctg
     1441 ggttaccgca tagtcgcaac gcacgccgcg gactttttcc acgtccgcgc gggtgacagg
     1501 ctgacggtag gcgatcaccg cgagcgtttc caatagggac tgcgaaacgc ccttggtctc
     1561 gtcgggattg agcagtttct ttaccgcgtc gatatagtcg cggttgctga cgagctgcgc
     1621 ggtgtccgcc gtaacaagca gctggatgcc gcgcccttcg cttttataga gctgttccat
     1681 ctcgctgagc gcgcctttgg caacggcgag cgagcagtcc agcacgcgcg caatctcgct
     1741 gacgggcacg gggtcgcccg ccacaaacag catgcactcg atggtgttga tcaaggttct
     1801 gtcttccata ccaatcccca aaactatccc aaattcaaat acgagttttc tattttaacg
     1861 aaggctcaat cttccgactc gtccatatat tcataggttt cgtcgtcaag aagcagcttg
     1921 tccgcgctga tgcgaattgg cgcaaacggc gcgctctgct tgagcagaat ttcgccgcgc
     1981 gcaatcatct ccagcagcgc cataaaggtg acgatcatct ccatgcgcgt cgtgttgctt
     2041 tcgaaaagtt cctcaaagaa cacggcggct ttctgctgca gaatcgtgcg gattttcccc
     2101 gtctgcgtgc ggacggtgta gcggtcgggg cgcacctgct gcgggccgat tcgctccggt
     2161 tcgtcctttt tgcgttccag cattactaga aacgccgaga acaatccgtc catactcgcg
     2221 ccgtccaggg tgacttcctt gggcggcagg ggcacgtcct ccggcaggcg cgttttgccg
     2281 tcttgagctg cttcgaataa ctgccgaagc tgttcggtcg cttccttgaa cgctttgtac
     2341 tcgcgcagtt ggcggatgag cagttcctcg gggtcttctt ccgctt
//
LOCUS       DAOMGY010000232         2380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_49632_length_2380_cov_3.037849, whole genome shotgun sequence.
ACCESSION   DAOMGY010000232 DAOMGY010000000
VERSION     DAOMGY010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49632_length_2380_cov_3.037849"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="PLR57_04405"
     CDS             complement(<1..1626)
                     /locus_tag="PLR57_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HQQ40728.1"
                     /translation="MASKNETAVSSGKKAAETRAKNREREKRASEVSGVLLIAGGLLA
                     AAYLFFSAAGYLGEMLGKAMFGLLGVISYALPVILIAVGVLYIRGSSNERLNGSGWYL
                     MLGILALVTLLQTIRTTTYEGVLYMNYINEAFNVGQTYHMGGGFIGAVLCYPLLMLGG
                     KTLAFILAISVILVCVIAITGFSIFDTGKKIRERIASAKESGDSSLAETREDGKKPMF
                     TLTLEDEAPPAKTGKRKTAAEPVAEVVPSKKKRAKPVEAERELQDFSGSFRTKDGQTP
                     QRREPEIVRPVFSEDIDFEPSGDLPVQKSSGKKGAPQPVQLPDPTPLFGAQLPKQAEK
                     PVKGPARQPGVKQQPIPAATKEKSQPEAIVSNAAYASPKIDLLEKADVAKEDENDSPE
                     EKARILIETLNNFRIAAQVTNIAVGPALTRIEIQPAPGVRISRITALQGDITMALAAP
                     RLRMEAPIPGKNAIGIEIPNKRSSLVVLRDIVESKEFRSSTSPVTMALGREASGKVIV
                     ADLTRMPHLLIAGATGSGKSVCINDIIISMVYKS"
     gene            2125..>2380
                     /locus_tag="PLR57_04410"
     CDS             2125..>2380
                     /locus_tag="PLR57_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HQQ40729.1"
                     /translation="MEIVFLDAYSFNPGDVGWEMFEELGHVTAFDLTPPEQIAERIAS
                     ADAVFTNRCRLGEAEFSAAPKLRFLGLTATGFDKIDLAAAR"
BASE COUNT          452 a          655 c          742 g          531 t
ORIGIN      
        1 gctcttgtag accatggaga tgatgatgtc gttgatgcag acgctcttgc cggaaccggt
       61 cgcgccggcg attaaaaggt gcggcatgcg cgttaaatcc gcaacgatga ccttgccgct
      121 ggcttcgcgg ccaagcgcca tggttacggg cgacgtgctg gagcggaact ccttgctttc
      181 cacgatatct ctcagcacca cgagcgagct gcgcttgttg ggaatttcta tgccgatggc
      241 gtttttgccg ggaatcggcg cttccatacg caggcggggc gccgcgagcg ccatggtgat
      301 atcgccctgc agtgccgtaa tgcgcgaaat gcgtacgccc ggcgcgggct ggatttcgat
      361 tcgcgtcagc gcggggccga cggcgatatt cgtcacctgc gcggcgatgc ggaagttatt
      421 gagcgtttcg attaagatgc gcgctttttc ttccggtgag tcgttttcgt cctctttggc
      481 aacgtccgcc ttttcgagca ggtcgatctt gggggaggcg taagccgcgt tggaaacgat
      541 cgcttccggc tgcgacttct ctttggtggc ggcgggaatg ggctgctgtt ttacgcccgg
      601 ctgacgcgcg ggacctttga caggtttttc cgcctgcttg ggcagctgcg cgccgaagag
      661 cggcgtggga tcgggcagct ggacgggctg aggagcgcct tttttgccag aactcttctg
      721 tacgggcaga tcgccgctgg gctcgaaatc gatatcctcc gagaagacgg gacggacgat
      781 ctccggctcc ctgcgctggg gcgtttgccc gtccttggtg cggaaactgc cggagaaatc
      841 ctgcagttcg cgctccgcct cgacgggttt tgcgcgcttc tttttggaag gcacaacttc
      901 cgcaaccggt tcggcggctg ttttccgctt gccggttttg gcgggcggcg cttcgtcttc
      961 aagcgtcagg gtaaacatcg gctttttgcc gtcctcgcgt gtttccgcca gagaagaatc
     1021 gccggattcc tttgcgcttg caatgcgctc acggatcttt ttgcccgtgt caaagatgga
     1081 gaagccggtg attgcgatca cgcagacgag gatgacggag atggctagaa taaaagcgag
     1141 cgtttttccg cccagcatca acagcgggta gcagagcacc gcgccgatga acccgccgcc
     1201 catatggtag gtctggccga cgttgaacgc ttcgttgatg tagttcatgt acagaacgcc
     1261 ttcgtaggtc gttgtacgaa tggtctgcag cagcgtgacc agagcgagga tgccgagcat
     1321 cagataccag ccggagccgt tcaggcgctc gttggaactg ccgcgaatat agaggacgcc
     1381 gacggcgatg aggatgaccg gaagcgcata ggaaattacg cccagcaggc cgaacatcgc
     1441 cttgccgagc atttcgccaa gatatcccgc cgcggagaaa aagaggtacg ccgccgcgag
     1501 gagccctccg gcgatgagca gcacgccgga tacttccgac gcgcgttttt cgcgttcgcg
     1561 gttcttcgcg cgcgtttcgg cggctttctt gccgctggag actgccgttt cgttttttga
     1621 agccaaagac ctctcaacct ttccggaacc ttgttccaag tcaaatgata tcgggtgttt
     1681 gtccgttcgc ctgttgtttg aattctggat gaagagggga aatccgcctt gaacgactgg
     1741 cctgggcggc gcgggcgggc ttatccaata aaaagagccg ccggatgaca atatccccaa
     1801 tttattcgta tattatacaa catattgcgg ttccctgcaa cgcggggcac gacaaagggg
     1861 aaacgtacaa caaaattacg caatttaagg cagattattc ttcaataatc attgcgtggc
     1921 tgaacgcgcg caggatggcc gcctgcttgt catccggcgg ggaatgcggt atactttttc
     1981 tacatcgggg ccggagtctg cgccctgtcc gccgcaccgg aaaagcgcgg aaatgggcag
     2041 gactgagcga ttggcggaaa cgccggatcg cttccaactg agccgggccg ccggtacaac
     2101 ctttgggagt agccagcata cgaaatggaa atcgtctttc tcgacgctta ctcgtttaac
     2161 cccggcgacg ttggctggga gatgtttgaa gaactcggac acgttaccgc gtttgacctg
     2221 accccgccgg agcagattgc cgaacggatt gcatctgccg acgcggtgtt taccaaccgc
     2281 tgcaggctgg gggaagcgga gttttccgcc gcgccgaagc tccggtttct ggggctgacc
     2341 gcgacggggt tcgacaagat cgaccttgcc gccgcaagaa
//
LOCUS       DAOMGY010000233         2376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_49760_length_2376_cov_3.411461, whole genome shotgun sequence.
ACCESSION   DAOMGY010000233 DAOMGY010000000
VERSION     DAOMGY010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49760_length_2376_cov_3.411461"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PLR57_04415"
     CDS             complement(<1..1082)
                     /locus_tag="PLR57_04415"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HQQ40730.1"
                     /translation="MRDERLDRLADLLLKYSLKLQPGELFEINGGVPAKPLIKALQKR
                     AHAIGAVPFVKLVDDEIARLFFGFIDPEHPEKSKPAIDAQLEWETKYWDHMVAHVDIG
                     VDENDAELSAVDTRSMTYYRNLRRELRDRIIDERRWVYLHWPTKADAQKAGMCYDDFF
                     EFFLSAALVDYEKMARDMEPLVALMEKTDLVHIKGPGTELTFSIKDIPVIPCHGELNI
                     PDGEVYTAPVRESANGTVQYNTPAMRYGKRFDNPRLVFKNGRIEEATCDGDVAGFNEM
                     LDADEGARYLGEFAIGVNNAVTRSIGNTLYDEKIGGSFHLTPGCAYADAFNGNKSQLH
                     LDIVCIQTAAFGGGEIWFDGQLIRKDG"
     gene            complement(1337..2035)
                     /locus_tag="PLR57_04420"
     CDS             complement(1337..2035)
                     /locus_tag="PLR57_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HQQ40731.1"
                     /translation="MYFGYGSTDSIYIILLLVVMGISLFAQNRVQKTFNQYAQTPISS
                     GLRAESVAQALLDRAGSSVQITSVQGSLTDHFDPKNQIVGLSQQVYGSNSIAAIAVAA
                     HEIGHVMQYEERYAPIRLRNAILPVASIGSYAAPWIVILGLMMGSYNLAMIGVVLFGA
                     MLVFQVVTLPVEFNASSRALRMLEEGNYITYEESDSAKKVLNAAAMTYVWAAVASLVS
                     FLRLLALANRTRRN"
BASE COUNT          499 a          660 c          691 g          526 t
ORIGIN      
        1 ccgtccttgc ggatcagctg gccgtcgaac cagatttcgc cgccgccgaa cgcagcggtc
       61 tggatgcaga cgatgtccag atgcagctgg gacttgttgc cgttgaaggc gtccgcgtag
      121 gcgcatccgg gggtcaggtg gaacgagccg ccgatctttt catcgtagag cgtgttgccg
      181 atggaacgcg tgacggcgtt gttgacgccg atggcgaact cgccgaggta gcgcgcgcct
      241 tcgtccgcgt ccagcatttc gttgaacccg gcaacgtcgc cgtcgcaggt ggcttcctca
      301 atgcggccgt ttttgaagac gagacgcggg ttatcgaagc gttttccata acgcatagca
      361 ggcgtgttat actgcacggt tccgtttgcg ctttcgcgta cgggagcggt gtaaacttcg
      421 ccgtcgggaa tgttcagctc gccgtggcag gggataacgg ggatatcttt aatggagaac
      481 gtcagttcgg tgccggggcc cttgatgtgc acgaggtcgg ttttctccat gagcgccacg
      541 agcggctcca tgtcccgcgc catcttttcg tagtccacca gcgcggcgga gaggaagaac
      601 tcgaaaaagt cgtcatagca catgccggcc ttctgggcgt ccgccttggt cggccagtgc
      661 aggtagaccc agcgccgctc gtcgatgatg cggtcgcgga gctccctgcg gaggttgcgg
      721 taataggtca tagagcgcgt gtccacggcg gagagttccg cgtcgttttc gtccacgccg
      781 atatccacgt gtgccaccat gtgatcccag tacttggttt cccattcgag ctgcgcgtcg
      841 atggcgggtt tggatttttc cggatgttca gggtcgatga agccgaagaa gagacgggcg
      901 atttcgtcgt ccaccagctt gacaaacggc accgcgccaa tggcgtgcgc gcgcttctgc
      961 agcgccttga tgaggggctt tgcgggtacg ccgccgttga tttcaaaaag ctcaccgggt
     1021 tggagcttaa gcgagtactt cagtaagaga tcggcaagcc ggtccagtcg ttcgtcgcgc
     1081 ataacaatcc tccttataca ctctgcccct tattctatag caaacaaagc cgcacttcaa
     1141 caccgcaaaa attttgcata aaaaagtgca aaggcgttac cttcgcactt tttacagtcg
     1201 tgttatggaa tcaaaactct tgcggggtta aggggcagtc agccccttac ggggtttggg
     1261 gcagagcccc aagtttaatc cggttaagag gcggtcagcc ccttgcgggg tttggggcgg
     1321 agccccaaga atcccctcag ttccttcttg tccggttggc cagcgccagc agacgcagaa
     1381 agctcacgag cgacgcgacc gccgcccaga cataggtcat cgcggcggcg ttcaatacct
     1441 tctttgcgct gtcgctttcc tcgtaggtga tgtagttgcc ttcctcaagc attcgcaacg
     1501 ctcggctgga ggcgttgaac tcgacaggca gcgtcaccac ctgaaacacc aacatcgcgc
     1561 caaagagcac aacgccgatc atcgcgaggt tgtagctgcc catcataagc cccaggatca
     1621 cgatccaggg cgcggcatag gaaccgatgc ttgccaccgg caaaatcgcg ttacggagcc
     1681 gtatgggggc gtagcgctcc tcgtactgca tcacatgacc gatctcatgc gccgcaaccg
     1741 cgatagccgc gatggagttg ctgccgtaga cctgctgcga cagcccgacg atctggttct
     1801 tggggtcgaa gtggtcggtc agcgagccct gtacggacgt aatctgcaca ctgcttcctg
     1861 cgcggtcgag cagcgcctgc gcgacggact ccgcgcgcag gccgctggaa atgggcgtct
     1921 gcgcatattg gttaaacgtc ttctgcacgc ggttttgcgc aaacaggctg atgcccatca
     1981 caacaagcag aaggatgata tagatcgaat cggttgaacc gtagccaaaa tacatggtgt
     2041 tccccctcct ttggttcgtt atcagtatag catagttgat attgcggtac agcgcttttc
     2101 acgatcgttt gaatttctta caggttttgt acaaaattgc ccgaaacaca caggcttttg
     2161 catttcgctt tccggcgagc agcaattgct tttttgccaa aaatccccct gttcacggcg
     2221 gtaaaatgtg gtatactagc aagaaatcca aagaaacata aaaagaaaca taagaggtta
     2281 tcgccatgac gctcaaattg tcacagattc aaaaatcgcc ggaagcgtac gcgggcaaga
     2341 tcagcgtatg cggctgggtg cgcaccgtgc gcgagg
//
LOCUS       DAOMGY010000234         2364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_50141_length_2364_cov_2.971416, whole genome shotgun sequence.
ACCESSION   DAOMGY010000234 DAOMGY010000000
VERSION     DAOMGY010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50141_length_2364_cov_2.971416"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLR57_04425"
     CDS             <1..836
                     /locus_tag="PLR57_04425"
                     /inference="COORDINATES: protein motif:HMM:NF019555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40732.1"
                     /translation="LELVNTDRLLSAVAGDSLNTVYPYAICGKTGDTDTAGKCFVAAA
                     EKDGARVIVALFGDKIDMYNGDKELTNLARFVNAASIFEYVFANKYQIVTASELGLQK
                     SFTTGVVNANEADLVNGQLTMTANTDNVTLRALPDKIASYKAAASSITANVVLTTDAK
                     APIIAGQSMGSVEYSLNGEVLLSVPLTADFAVSEVLEMSSVQPDETAVVETSPGSTPL
                     ISKGHKEWGTADVLTLVFVLLLVLLVALIVVFIITERKRRYERKRRSARARARKKNYY
                     K"
     gene            complement(1006..1809)
                     /locus_tag="PLR57_04430"
     CDS             complement(1006..1809)
                     /locus_tag="PLR57_04430"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HQQ40733.1"
                     /translation="MGNCEGIYLYCDLDGTLLNDEKRVSAEDSAAIAEFVARGGRFGV
                     ATGRVPGIIGEIERELPVNAPCILYNGAGLYDLSQRRFLAMHPVDRKACARIAQKAMR
                     LYPDVCVQIFTDSAIYEVNPNQRDDPQTLVERIPTVKCPLEEVPDIFLKFILSLDPAR
                     LRELVAKIDLPHLDERLSAFQSSDVYLEFVSKGVCKGMALGDVRTRCENVQKILAVGD
                     YENDLEMIALADIGAAPSNAIDVVKRAADIVLPVDNNHSAVAKFLEQVL"
     gene            2173..>2364
                     /locus_tag="PLR57_04435"
     CDS             2173..>2364
                     /locus_tag="PLR57_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40734.1"
                     /translation="MNQQVLRYVAGVGAANVDVYGRSRSPIVMRDSNPGYLGTSVGGV
                     TRNILENLARQGLDTVLLSA"
BASE COUNT          548 a          646 c          688 g          482 t
ORIGIN      
        1 agcttgaact agtcaataca gaccgcttgc tcagcgccgt agctggcgat tcgctcaata
       61 ccgtctatcc ctacgccatc tgcggaaaaa cgggagatac cgatactgcg ggcaagtgct
      121 ttgtcgccgc agcggaaaag gacggcgcgc gcgtaattgt agctctgttt ggcgacaaga
      181 tagacatgta caatggcgat aaagaactca ccaatctcgc gcgatttgtc aacgcggcga
      241 gcatatttga atacgtcttt gccaacaagt accagatcgt aaccgcttcg gaacttggcc
      301 ttcaaaagtc gtttacaacc ggagttgtca acgcaaacga agcggacctg gtcaacggtc
      361 aactcactat gaccgcgaac accgacaacg tgactctacg cgcgcttccg gataagatcg
      421 cttcctacaa ggccgccgct tcctctatta ccgcaaacgt cgtgctgacc acggacgcaa
      481 aagcgccgat tatcgcgggg cagagcatgg gcagcgtgga atacagctta aacggcgaag
      541 tgctcttgtc tgtgccgctg acggcggatt tcgcggtaag cgaagtgctg gagatgtcct
      601 ccgttcagcc ggacgaaacg gcggttgtgg agacatcacc tggcagcacg ccgctcatct
      661 ccaaggggca taaggaatgg ggcacggcgg acgttctgac gctggtgttt gtgctgctgc
      721 tggtgcttct ggtggcgctg atcgtggtgt tcatcatcac cgagcggaaa cgccgctatg
      781 agcgcaaacg ccgcagcgcg cgcgcccgtg cccgcaagaa aaactactac aaataaaaca
      841 caaaccgccg cagatcgcgg cggttttgtt tattattctt ttacgagggc gctgccctcg
      901 agctcccgcc aaagggcaag ccctttggaa acccttttga tgagcgagtg gggttctcag
      961 gggcgtgccc ctgagcggtg gttcggaggc agagcctcag agagcctaca gcacctgttc
     1021 caaaaacttc gctaccgcgc tgtggttgtt gtctacgggc aggacgatat ccgccgcgcg
     1081 cttgaccacg tcgatggcgt tggaaggcgc ggcgccgata tccgccagcg cgatcatctc
     1141 cagatcgttt tcgtaatccc cgactgcaag aatcttctgg acgttttcgc accttgtccg
     1201 aacgtcgccc agcgccatgc ccttgcagac acctttggag acgaactcca gatacacgtc
     1261 cgaggactga aacgcggaga gcctctcatc taaatgcggg aggtcgattt ttgcgaccag
     1321 ttcgcgcagg cgggcgggat caagggacag aatgaattta aggaagatat ccggcacctc
     1381 ttcgagcggg cacttgacgg tcggaatgcg ttcaacgagc gtttgtggat cgtccctctg
     1441 gttgggattg acttcataga tggcggagtc ggtaaaaatc tggacgcaga cgtcgggata
     1501 cagccgcatc gctttttgcg caatgcgcgc gcaggctttg cggtcaacgg ggtgcatggc
     1561 taaaaaccgt ctctgcgaaa ggtcgtagag ccccgcgccg ttatacagaa tgcagggggc
     1621 gttgacgggc agctcgcgct cgatctcgcc tatgatgccc ggaacgcggc ccgttgcaac
     1681 gccaaatcgc ccgccgcgcg caacaaactc cgcaatcgcc gcggaatctt cggcagaaac
     1741 gcgcttttcg tcgttgagca gggtaccatc caaatcgcag tataaataaa tgccttcgca
     1801 gttgcccatg aaacgtcctc cggaaaaagt acagcaatgg ggaaatccgg cgcgtcagat
     1861 cggtctgtct cgcggaaaag cccaattgcg taacattcta taccagcgga acagaaaacg
     1921 caacgcggac ggaagaattc ggcggaatcg gaagaaagat agaaagcatt gtacgatacg
     1981 cggttccgca ttgcgcggtg aacacagaat tgctcaatat gcgccatttt tgttcacgcc
     2041 gggcggtctt gcaaaaccgg ctcatctgcg ggtatactag gagcgcggcg ggagtaaacc
     2101 cgccggagaa ccggacgctt ttacagttgt tccggttgat tcttttcgga cggaaaagga
     2161 gaacagagcg ggatgaacca gcaggttttg agatatgttg cgggcgtagg cgccgcgaac
     2221 gtggacgttt acggcaggag cagaagcccc atcgtcatgc gggactcaaa ccccggctat
     2281 cttggaacgt ccgttggcgg agtgacgaga aacatcctcg aaaaccttgc gcgtcagggg
     2341 ttggataccg ttttgctttc cgcc
//
LOCUS       DAOMGY010000235         2349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_50617_length_2349_cov_3.456408, whole genome shotgun sequence.
ACCESSION   DAOMGY010000235 DAOMGY010000000
VERSION     DAOMGY010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50617_length_2349_cov_3.456408"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLR57_04440"
     CDS             complement(<1..661)
                     /locus_tag="PLR57_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40735.1"
                     /translation="MSYCVNCGVELQKSEPRCPLCGTEVVNPSAPQEEKPVRPYPSHV
                     ERLDQRLDRRYIASFISLLMLIPLFSVMLANILVSHRLTWSYYVLGGELVAFTVFLLP
                     MIARMPRILYVTIDAAAIGVLLLLVQVMAQGNWNWLLLLGLPITGLTALMCWFFSLLA
                     APKCRLPILIRFTFGLTCLGLLVVAIEFFINLYQMRLAWPTWSLYVLFPCLVLGSSLL
                     LL"
     gene            complement(798..2087)
                     /locus_tag="PLR57_04445"
     CDS             complement(798..2087)
                     /locus_tag="PLR57_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40736.1"
                     /translation="MQKKQKSRPWMKLDNAALIYPATMNRSWTALFRLSATLTEPIDE
                     DILEVAQKRTLRRLPWFSYRLKKGLFWFYLDHAEHDPKIQPDVGNPCVRMRLRENGGF
                     CMRVRHYQNRIAVEFFHVLTDGTGGLVFLQTLVAEYLRLRYGAKIPRSEKILDCSEPP
                     RPQEAEDSFATNSGTVTQSRSEPKAYHISGTDERGGFVHIITGSMDSDVVLQKAREKG
                     VTITEYLTAVMILSVDAIQRRQVTHVHRFKPVKISVPINLRNVFPSSTLRNFANYVNP
                     GIDPRLGTYTFDEVLNLVHHFMAMEVTKKLLNVKITTNVRTEQNRVLRLMPLFIKNMA
                     MKYVYSQVGDVLSSTTLSNLGVTKLPEEMAQYVRRMDFILGPLASNRVCAAALTYNGR
                     LRINFTRTIAEPLLEREFFTRLVKQGIPVKVESNQME"
BASE COUNT          521 a          650 c          676 g          502 t
ORIGIN      
        1 tgagaagcag gagcgaggag ccgagcacaa gacagggaaa gagcacatac agcgaccacg
       61 ttggccatgc aagccgcatc tggtagaggt tgatgaagaa ctcaatcgcc accacgagca
      121 gacccaggca ggtcagtccg aacgtgaacc ggattaggat cggcaggcgg cactttggcg
      181 cggcaagaag gctgaaaaac caacacatca gcgcggtaag gccggtaatc ggcagtccca
      241 gcagtaggag ccagttccag ttgccctgcg ccattacctg cacgagcagc agcagcacgc
      301 cgatggccgc cgcgtcgatg gttacataca gaatccgggg catgcgcgca atcatcggca
      361 gcagaaacac cgtaaacgcg accagctcgc cgccgagcac atagtaactc caggtcagcc
      421 tgtggcttac gaggatgttg gccagcatga ccgaaaacag cggaatcagc attaaaagcg
      481 agataaacga cgcgatatac cgccggtcga gacgctgatc cagccgctcc acatggcttg
      541 ggtacgggcg gacgggcttc tcctcctgcg gggcggaggg gtttacgacc tcggttccgc
      601 agagcgggca gcgcggttcg cttttttgaa gttctactcc acagttgacg cagtaactca
      661 tattgaactc tccttcgaac accgcgtttg gcttttattc aaaagttcga caccccagtt
      721 tacgcagtaa ctcataaaga acactccttt ggggaaacac ttaaaaactc ggaacgacaa
      781 taacaaaatt ccggaagcta ctccatctgg ttgctttcca ccttcacggg gatgccctgt
      841 ttgacaagcc gcgtaaagaa ctcgcgttcc agcagcggtt cggcaatggt gcgcgtaaag
      901 ttgatgcgaa gcctgccgtt gtaggtcaag gcggcggcgc agacgcggtt gctcgccagc
      961 gggccgagga taaagtccat gcggcggacg tactgcgcca tctcttccgg cagtttggtc
     1021 acgccaagat tactcaacgt tgtggaagaa agcacgtcgc ccacctgcga atagacgtac
     1081 ttcatggcca tattcttgat aaacagcggc atgagtctaa gaacccggtt ctgctcggtg
     1141 cggacgttgg tggttatctt gacgttgagc agtttcttcg tgacttccat cgccatgaag
     1201 tgatggacga ggttgagcac ttcgtcgaac gtatacgtgc caagccgcgg gtcgatgcca
     1261 gggttgacat agttggcgaa attccgaagc gtgctggaag gaaagacgtt tcttaggttg
     1321 atcggcacgc tgattttgac cggcttgaac cggtgcacgt gcgttacctg cctgcgctgg
     1381 atagcgtcca cggagagaat catcaccgcg gtgagatact ccgtaatggt aacgcctttt
     1441 tcgcgcgctt tctgcagcac gacgtccgaa tccattgagc cggtgatgat atgcacaaac
     1501 ccgccgcgct cgtccgtgcc gctgatatgg taagctttcg gttcactgcg ggactgtgtg
     1561 acggttccgg aattggttgc aaagctgtcc tccgcttcct gcggtctggg cggctcagaa
     1621 caatcgagaa tcttttcgct gcgcggaatt tttgccccgt agcggaggcg cagatattcg
     1681 gcaacaagcg tctgtaaaaa gaccagcccg cccgtgccgt cggtaagcac atggaaaaac
     1741 tccaccgcaa tgcggttctg atagtggcga acgcgcatgc agaacccgcc gttttctctg
     1801 aggcgcattc tcacgcaggg gttgccgacg tccggctgaa tcttcgggtc gtgctcggca
     1861 tggtcgaggt aaaaccagaa caaccctttt ttgagccggt aggaaaacca gggcagccgc
     1921 cggagcgtcc gcttttgcgc tacttcaagg atatcctcgt cgatgggttc cgtcagcgtt
     1981 gccgaaaggc gaaaaagcgc cgtccagctg cggttcatcg tcgccgggta gatcaacgcg
     2041 gcgttatcca gcttcatcca gggcctagat ttttgtttct tctgcatctg ttatccgatc
     2101 gcgttcccgt cggcagttgc ctgcggggaa acgcgctcct ttttaacttt gctgtttgaa
     2161 tgcgtgttta tatcaggccg tcgggcggat ctatcggccg gaaaccgctg cgctcaatcc
     2221 aagctcggtg aggatgaatt cggacatctg cgcaaccgcc tttttgcctt ccggcgtgcc
     2281 gtaaagcggg tagacgtgcc aaagtccgtt tccgatcacc agctcgacct gcacgccgcc
     2341 ggcgctgag
//
LOCUS       DAOMGY010000236         2342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_50838_length_2342_cov_2.407521, whole genome shotgun sequence.
ACCESSION   DAOMGY010000236 DAOMGY010000000
VERSION     DAOMGY010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50838_length_2342_cov_2.407521"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PLR57_04450"
     CDS             <1..1118
                     /locus_tag="PLR57_04450"
                     /inference="COORDINATES: protein motif:HMM:NF027792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5054 domain-containing protein"
                     /protein_id="HQQ40737.1"
                     /translation="KKHLLDFTSWTKADFSRARAADRTDYSFFGVRNRHIFEAVREEL
                     REYRGENEVSSYSHFTRSHLEQRAYVDAALNALPAPLRAEAEGSLAFSFPEKAGDSCA
                     YERMISVGGWNVFIARNGALTRVQNKALGIDRRVNIGAFCYESFGGKESEDCFFDYVR
                     DAEKNFAWAACDFGKPGLRYETSIKHKLWDVCADELRQMGDTLTVFLHGEEEAVETYG
                     CPRELALSYRFLLDSIEVTLYWHGKDAVRSPEALWLGFDLCANNPNRWTMRKLGNPVS
                     PLNVASGGNRRLHAVERLSCQTALEELEIAPLDAPLVSVGGRYLYDTADEVGGMQNGF
                     WFLLCNNRWGTNFPQWFEDDMRFTFTVSLKELAPAVR"
     gene            complement(1175..1504)
                     /locus_tag="PLR57_04455"
     CDS             complement(1175..1504)
                     /locus_tag="PLR57_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HQQ40738.1"
                     /translation="MATFDDFMKIDIRVGEVVRAEVFEKAKKPAYKLWVDFGEEIGTK
                     QSSAQITQLYSAEELVGRQVLGVVNFPPRQVADFFSEVLVLGVYAEQGVVLIQPDQTV
                     RKGDKLG"
     gene            complement(1664..>2342)
                     /locus_tag="PLR57_04460"
     CDS             complement(1664..>2342)
                     /locus_tag="PLR57_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQQ40739.1"
                     /translation="LATDVTEPQRNLFIQYSDSVSVPVVVVDNFLFSAYTDCVGNDNT
                     YGVKSAVSYLIECGHRKIGYLRSKQRVVNFDDRELGVQLSLAEHKELGLDPLQVINVD
                     ISPDQAYRDVIAWLNAGNVPAAAFFAENDMLAATFIRALKECGYRVPDDVSVIGFDDV
                     QICEMVEPPITTMQAFKERLGRVATTLLDFRIRSGDTLQTSPHSGLMKIALSLQLKER
                     ASVKKIG"
BASE COUNT          481 a          663 c          703 g          495 t
ORIGIN      
        1 ccaagaagca cctactcgat tttaccagct ggacaaaggc ggatttctcc cgcgcgcgcg
       61 cggcggacag gacggactat tcgttttttg gcgttcgtaa tcgccatatt ttcgaagcgg
      121 tgcgcgaaga actgcgcgaa taccgcgggg aaaacgaagt ttccagctat tcgcacttta
      181 cgcgctccca tctggaacag cgcgcgtacg tggatgcggc gctgaacgcg ctgcctgctc
      241 cgcttcgcgc ggaggcggaa ggatcgctgg cgttttcatt tcccgaaaaa gcaggggatt
      301 cctgcgcgta cgaacgcatg atttcggtcg gcggctggaa cgtcttcatc gcgcgaaacg
      361 gcgcgcttac gcgggtacaa aacaaagcgc tcggcatcga ccggcgcgtg aacatcggcg
      421 cgttctgcta cgagtcgttt ggcggaaagg aatcggagga ctgctttttt gactatgtgc
      481 gggacgcgga aaagaacttc gcatgggcgg cctgcgactt tggaaagcca gggttacggt
      541 atgaaacgtc gatcaagcac aagctttggg atgtctgcgc cgatgagctg cggcagatgg
      601 gggacacgct gaccgtattc ctgcacgggg aagaggaagc ggtggaaact tatggctgcc
      661 cgcgcgaact cgcgctgtcg taccgctttt tgctggattc gattgaagtc acgctctact
      721 ggcacggcaa agacgctgtc cgcagcccgg aagcgctctg gctcgggttc gatctttgcg
      781 cgaacaaccc gaaccgttgg acgatgcgga agctcggcaa tccggtttcg ccgctgaacg
      841 tcgcctccgg cggaaaccgc aggctgcacg ccgtggagcg gctttcctgt caaaccgcgt
      901 tggaggagct ggagattgcg ccgctcgatg cgccgctggt cagcgtcggc gggcgatatc
      961 tctacgacac ggcggacgaa gtggggggta tgcaaaacgg gttctggttt ctgctgtgca
     1021 acaaccgctg ggggacgaac tttccgcagt ggttcgagga cgatatgcgc tttacgttca
     1081 ccgtttcgct caaagagctt gcgcccgccg tacggtaatc gggtgaatat aaaaaacagt
     1141 ccgcgcgtgc ggactgtttt ttatttgcga tgcttcagcc gagcttgtcg ccttttctca
     1201 cggtttggtc gggctggatg agtacgacgc cctgctcggc gtaaacgccc agcacgagca
     1261 cttcggaaaa gaagtccgcc acctgccgcg ggggaaagtt caccacgccc agcacctgcc
     1321 gtccgaccag ttcctcggct gaatagagct gcgtaatctg cgcgctgctt tgcttcgttc
     1381 caatctcttc gccgaaatcc acccagagct tataggcggg tttcttcgcc ttttcaaaca
     1441 cctcggcgcg gacgacttct cccacgcgga tgtcgatctt cataaaatca tcgaaggttg
     1501 ccatgacaat acttcctttg ccggatttga ataagagcaa aaactacctg ctccggtgcg
     1561 ccgcaacgca ttgggcttat tgtaataccg gccgccggct gcgtcaatgt tgacgcgtac
     1621 aaaaaagccg cccaaaaggg cggcgggatg aagttttcga attctacccg atcttcttga
     1681 cgctggcgcg ttccttgagc tggagcgaaa gcgcaatctt catcaggccc gaatgaggag
     1741 aggtctgcag cgtgtccccc gaacggatgc ggaagtccaa aagcgtggtt gcaaccctgc
     1801 cgagccgttc cttgaacgcc tgcatggtcg taattggcgg ctcgaccatt tcgcagatct
     1861 gcacgtcgtc gaagccgatc accgaaacgt cgtccggcac gcggtagccg cactccttga
     1921 gcgcgcggat aaacgttgcc gcgagcatat cgttttcggc aaagaacgcc gccgcgggga
     1981 cgttgcccgc gttcagccag gcgatgacgt cccgatacgc ctgatcgggc gaaatatcga
     2041 cgttgatgac ctgcagcggg tcgagcccca gctccttatg ttcggccagc gaaagctgaa
     2101 cgcccagctc gcggtcgtca aagttgacga cgcgctgctt agagcgaagg tatccgattt
     2161 tgcggtgccc gcactcaatg aggtaggaaa ccgcggattt tacgccgtag gtgttgtcgt
     2221 tgcccacgca gtccgtatac gccgaaaaca ggaagttatc gacaaccacg accggaacgc
     2281 tgacgctgtc gctgtactgg ataaagaggt ttctctgcgg ttcggtaacg tccgttgcca
     2341 gc
//
LOCUS       DAOMGY010000237         2335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_51050_length_2335_cov_3.619298, whole genome shotgun sequence.
ACCESSION   DAOMGY010000237 DAOMGY010000000
VERSION     DAOMGY010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51050_length_2335_cov_3.619298"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /gene="yedE"
                     /locus_tag="PLR57_04465"
     CDS             complement(<1..867)
                     /gene="yedE"
                     /locus_tag="PLR57_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HQQ40740.1"
                     /translation="MKKRNLWVILTGVVIGLAALLLTALGNPGNMGFCIACFIRDTAG
                     AAKLHSAEVVQYVRPEIVGMIAGSMIAAMLFKEFKPRGGSSPMTRFVIAVIVMIGALM
                     FLGCPLRMMLRIGGGDLNALVGLVGFVGGIFVGTLWLKGGFSLKRAYPQSLTEGGLLP
                     IISIALLVLSVTIPALFAFSESGPGAAHAPVLIALAAGLLVGGLSQRSRFCTVAGSRD
                     AMMFKDFSMLWGFLAVIATTIVGNLILGKFNLSFADQPVAHTDGLWNFLGMALVGWGS
                     VLLGGCPLRQVIL"
     gene            1057..2229
                     /locus_tag="PLR57_04470"
     CDS             1057..2229
                     /locus_tag="PLR57_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQQ40741.1"
                     /translation="MSEKNFAAPIIYLDQAATSRPKAKGVGEAMRTYIEDVCVNVSRS
                     AYAPSTAAAINALETRELLCELFGFGDPTYVIFTPGHTVSLNMVLKGILRPGDHALVS
                     SLEHNAVMRPLVQLTESGVTFDRIPVRGDDELDLDAAERMIRPNTKLCIVSHASNVSG
                     TILPIDALSKLCRQKGIFFAVDAAQTAGHYPIDLRKIAVDALCVPGHKGLQGPGGIAA
                     MLLSSRFASALEPLITGGTGSDSNSEIQPRYMPDRFESGTLNLAGIYGLHAALSELLE
                     QGVPARRKRELALLERFTAGLSRMEGFRTIGASDLSRRVGVVSCDFAKLDNAVVADRL
                     SSDYGVLTRCGLHCAPNAHKTYGTFPQGTVRFSMSHANTEQEIDAALAALAEILKD"
BASE COUNT          509 a          707 c          698 g          421 t
ORIGIN      
        1 gaggataacc tgccgaagcg ggcagccgcc gagcagaacg ctgccccagc cgacaagagc
       61 cataccgagg aaattccata gcccgtccgt atgcgcaacc ggctgatcgg caaaggagag
      121 gttgaacttg ccgagtatga ggttgccgac gatggtggtg gcgatgaccg cgaggaagcc
      181 ccagagcatg gagaaatcct tgaacatcat agcgtcgcgg ctgccggcca cggtgcagaa
      241 gcgcgaacgc tggctgagac cgccaacgag cagacccgcc gccagcgcaa tcaacaccgg
      301 tgcgtgcgcg gcgcccgggc cggattcgct gaaggcaaac agcgcaggaa tcgttacgct
      361 gagaacgagc agcgcaatgg aaatgatcgg cagcaggccg ccttccgtca ggctctgcgg
      421 atacgcgcgc ttgagcgaaa aaccgccctt gagccagagc gtgccaacga agatgccgcc
      481 aacaaagcca accagcccga ccagcgcgtt gagatcgcca ccgccgattc tgagcatcat
      541 gcgcagcggg cagccgagga acatcagcgc gccgatcata acgattaccg caatgacaaa
      601 ccgcgtcatc ggcgaagagc cgccgcgcgg cttgaattcc ttaaagagca ttgccgcgat
      661 catggagccc gcgatcatgc ctacgatttc cggacgaacg tactgcacta cctctgccga
      721 atgcagcttg gccgctcccg ccgtgtcccg aatgaagcac gcgatgcaga agcccatgtt
      781 gccggggttg ccgagcgcgg tgagcaaaag cgccgccaaa ccaatgacaa cgccggtgag
      841 aatcacccac aagtttcgtt ttttcatgtt gtttcctatc cttccctgat tgtaaaatgt
      901 aagcaatgac ggcggagttt tctccgtctg ctggatgtgc ggcgcttctc gtgatacgat
      961 agattatgga aagaacgccg cctgaacttg attgatctgc agatttcgga tatactgtac
     1021 catatcaaaa cgttatacgc aagcgaggat aacgctatgt cagaaaaaaa ttttgccgcc
     1081 ccgatcattt atctcgatca ggcggctacc tcccgcccca aggcaaaagg cgttggcgag
     1141 gcgatgcgaa cgtatattga ggacgtctgc gtcaacgtca gccgctcggc ctacgcgccc
     1201 tccacggcgg cggccatcaa cgcgctggaa acgcgggaac tgctctgcga actgttcggc
     1261 tttggcgacc ctacttacgt catctttacc cccgggcaca cggtttcgct caacatggtg
     1321 ctcaagggca tcctgcgccc cggcgatcac gcgctggtaa gctcgctgga gcacaacgcc
     1381 gtgatgcgcc cgctcgtgca gctgactgag tcgggcgtga ctttcgaccg catccctgtg
     1441 cgcggggacg acgaactcga cctcgacgcg gcggagcgga tgattcgccc caatacaaaa
     1501 ctttgcattg tgagccacgc cagcaacgtc agcggcacga ttctgcccat tgacgcgctc
     1561 tccaagctct gccggcagaa gggaatcttc tttgccgtgg acgcggcgca gaccgcgggg
     1621 cactacccca tcgatctgcg gaaaatcgcc gtggacgcgc tctgtgtgcc ggggcataag
     1681 ggtctgcagg ggccgggcgg aattgcggca atgctgctta gttcccgctt tgcctccgcg
     1741 ctagagccgc tgattacggg cggcacaggg agcgattcca acagcgaaat ccagccaagg
     1801 tacatgcccg accggttcga gtctggcaca ctgaaccttg cgggcattta cggcctgcat
     1861 gccgcgctga gcgagcttct tgagcagggc gttcccgcac gcagaaaacg cgagcttgcg
     1921 ctgctggagc gcttcaccgc cgggctgagc aggatggaag gattccgtac catcggggca
     1981 agcgacctct cccgccgcgt gggagtcgtc tcctgcgact ttgcgaagct ggacaacgcg
     2041 gttgtcgccg accggctttc gagcgattat ggggttctca cgcgttgcgg gctgcactgc
     2101 gcgccgaatg cgcacaagac ctatggaaca tttccgcagg gcacggtgcg gttttcgatg
     2161 agccacgcga acaccgagca ggagatagac gccgcgctag ccgctctcgc ggaaatcctg
     2221 aaagactaaa ccaggccgca tcaaaaaatc cgccttccgg cggatttttt gtgtgcgtat
     2281 atctagtaag gttctaaggg gcgtgcccct tagcgggagc tagagggtgg aaccc
//
LOCUS       DAOMGY010000238         2326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_51377_length_2326_cov_3.340379, whole genome shotgun sequence.
ACCESSION   DAOMGY010000238 DAOMGY010000000
VERSION     DAOMGY010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51377_length_2326_cov_3.340379"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PLR57_04475"
     CDS             complement(<1..671)
                     /locus_tag="PLR57_04475"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HQQ40742.1"
                     /translation="MSMDGIALGAVIAELQPLIGGKIDKVQQPEKDLLLFVVRASSGT
                     QRLLVSTHPENGRIQLTCQSYDNPAQAIAFCMLLRRRLTGGRICSIRQVGTDRVCEIV
                     IQARNELFDEVSLKLVIELTGKHGNLMLLEQNGCIVDCVRRVSAGDTTSRILLPGFPY
                     EPIPQQEKLDPFTAEPEQFEPVFASGDPVRALMNSFGGISKQTAAALLSACSTPSALA
                     DLFSQ"
     gene            complement(704..1006)
                     /locus_tag="PLR57_04480"
     CDS             complement(704..1006)
                     /locus_tag="PLR57_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HQQ40743.1"
                     /translation="MEPYVSRLESEELDDVFAAILSLNDVSECYRFFEDMCTINELQA
                     MAQRWQVARQLDAGVTYQEISHLYNASTATISRVNRCLEYGAGGYRLVLDRMKKPE"
     gene            complement(1185..1448)
                     /gene="rpsT"
                     /locus_tag="PLR57_04485"
     CDS             complement(1185..1448)
                     /gene="rpsT"
                     /locus_tag="PLR57_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQQ40744.1"
                     /translation="MANIKSAIKRVKVTEKQTLRNRAAKSEMKTTIRRFDEAMQSGDA
                     AATDKAYLTAVSTVDHAAAKGMIHKNAAARKKAQLAKKRAAAQ"
     gene            1805..2089
                     /locus_tag="PLR57_04490"
     CDS             1805..2089
                     /locus_tag="PLR57_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQQ40745.1"
                     /translation="MKLKPLADRVVVVSIENEEVVKGGIVLPTAAKEKPQMAEVISVG
                     PGGIVDGKEIKMYVKEGDKVICSKYSGTEVKLDDKEYLIVRQGDILAIVE"
     gene            2111..>2326
                     /locus_tag="PLR57_04495"
     CDS             2111..>2326
                     /locus_tag="PLR57_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HQQ40746.1"
                     /translation="MAKQIKYGEEARVALKSGLDQLADTVKITLGPKGRNVVLDKKYG
                     APLITNDGVTIAKEIELEDPFENMGAQL"
BASE COUNT          575 a          604 c          651 g          496 t
ORIGIN      
        1 atttggctga acaaatccgc cagagcggac ggggtggagc aggcggaaag caaggcggca
       61 gcggtctgct tggagatgcc gccaaatgag ttcatcagtg cgcggacggg gtcgccggaa
      121 gcaaagaccg gctcgaactg ctccggctcc gccgtaaacg ggtcgagctt ttcctgctgc
      181 ggaatcggct cgtacggaaa gcccggcaaa aggatacgcg aggtggtgtc cccggcggaa
      241 accctgcgca cacagtccac gatgcagccg ttctgttcca gcagcatcag gttgccgtgc
      301 ttgccggtca gttcgatgac gagcttgagg gagacttcgt cgaacagctc gttgcgggct
      361 tgaatgacga tttcgcagac gcggtccgtg ccgacctgcc taatagagca gatgcgcccg
      421 ccggtgagcc tgcgccttag cagcatgcaa aaggcgattg cctgcgcggg gttgtcgtaa
      481 ctttgacagg tcagctggat gcgcccgttt tccgggtgcg tggaaacgag caggcgctgc
      541 gtgccggaac ttgcgcgcac aacaaagagc agcaggtctt tctccggctg ctgcacttta
      601 tcaatcttgc cgccgatgag cggctgtaat tcggctatga ccgcgccgag cgcgattccg
      661 tccatggaca tacgagttta ctgctccaat atgataaagg tagttattcg ggttttttca
      721 tgcggtcgag cacaagccgg tagccgccag cgccgtattc caaacagcgg ttcacgcggc
      781 tgatggttgc ggtagaggca ttgtagagat gcgaaatctc ctgatacgta acgcccgcgt
      841 cgagctggcg cgcaacctgc cagcgctgcg ccatcgcctg cagttcgttg atggtgcaca
      901 tatcctcaaa aaaacgatag cattccgaaa cgtcgttcag cgagagaatg gcggcaaaga
      961 cgtcgtccag ttcttcgctc tccagccggg aaacatatgg ctccatagaa tcgcaccttt
     1021 catagaaata ttcaagcaat gattttcggt tgatgccgac tgccgcggat ttgaataacg
     1081 gcagcgggaa agggacaact caattctagc cgctttcaca ctggaaagca agaaaaacag
     1141 gcgctccgtt tccggtgcgc ctgtcaagag ccgatcgatc ggggttactg cgcggcagcg
     1201 cgctttttcg ccaactgcgc ttttttgcga gcggcggcat tcttgtggat catgcctttc
     1261 gcggcggcat ggtcgaccgt gctgacggcc gtcaaatacg ctttatctgt tgcggcggcg
     1321 tcgccggact gcatcgcttc gtcaaaacgg cggatcgtcg tcttcatttc agacttagcc
     1381 gcgcggttgc gaagggtctg cttttccgtg accttgacac gcttgatggc ggacttgatg
     1441 tttgccaata tcattcaccc cctgtatcag tctcaaattc attcgaacag atgttagttt
     1501 aacatacact tactgcgaat gcaagcgtaa tcttgaattt agtcggcatt cgcggcagga
     1561 aacaccgctt ttttatataa ggtagaagca cgctccaaac gaagcataga atatcaaaac
     1621 agcgcgggaa acacaagaga ttctcgtgct ttcgggcggt acatttcaca ttttcgttgc
     1681 atttttcaag ttgacttgaa aagcagtcgt ggtataatga aaaccgtgat tagcactcga
     1741 tccaaaagag tgctaacaga cagaaatcaa acaaatcact cataaactag gaggcgtaaa
     1801 tcgtatgaaa ctgaaacctt tggcagaccg cgttgtcgtt gtcagcatcg aaaacgaaga
     1861 agtggtcaag ggcggcatcg ttctgcctac cgccgcgaaa gaaaaaccgc agatggcgga
     1921 agtcatctcc gtgggccccg gcggcatcgt ggacggcaag gaaatcaaaa tgtacgtcaa
     1981 ggaaggcgac aaggttatct gttcgaagta ttcgggaacc gaagtcaagc ttgacgacaa
     2041 agaatacctg atcgtccgcc agggcgacat tctcgcgatt gttgaataac taaaggaggc
     2101 ataaaacact atggcaaagc aaattaagta cggcgaagaa gcacgcgtcg ccctgaaatc
     2161 cggcctggat caactggctg ataccgtaaa aatcacgctg gggcccaaag gccgcaacgt
     2221 ggtgctcgat aagaaatacg gcgctccgct gatcaccaac gacggcgtga ccatcgccaa
     2281 ggaaatcgag ctggaagacc cattcgaaaa catgggcgca cagctc
//
LOCUS       DAOMGY010000239         2324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_51443_length_2324_cov_3.041869, whole genome shotgun sequence.
ACCESSION   DAOMGY010000239 DAOMGY010000000
VERSION     DAOMGY010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51443_length_2324_cov_3.041869"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PLR57_04500"
     CDS             <1..908
                     /locus_tag="PLR57_04500"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ40747.1"
                     /translation="TDTLLRTLLKCRNRCPVMFASSAQAALPNLYGRSKKAGEELLFQ
                     YQRETDAKIYVFRFPFVFGKWSKPNYNNPVATFCYNVSHDLPVVVSDPAALISLAYVD
                     DLAEALVELIDETPVAMDGPYCAVPLVYTASLGDVADQIRSFAKIRTENVLPDMSSPL
                     TRKLYATYLSFLPTMEFAYPLFAKEDEQGMSAEFLRLCAGGQISVCIVKPGAVKGNHW
                     HHARTEKVLVVSGAGVIRFCSPGTDDVMEYHVSGKKFVVVDIPVGYAHNLENTGKTDL
                     VALLWASEWTDPARPDDVDSVLPIY"
     gene            1026..2132
                     /locus_tag="PLR57_04505"
     CDS             1026..2132
                     /locus_tag="PLR57_04505"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HQQ40748.1"
                     /translation="MARSESGMPEQKAIPEPKANILLLGGTGPLKDAAAPVLSEDVKR
                     LFGASGFRCGALFCDRNTHGGAGETGQNAIEWLKNTGIGLVSLCGVLQEDLQGTLDAL
                     DGVKTLGVGANQALANRAEVVSVNGVRLGFIALNECSGDRERFAPEADILEADALDRV
                     RKLLPQCDHIIVFSRLGIPGYGLPLPEWRARCRRFIKAGASVVAVMEPDALMGWEEYE
                     NGLILYGLGTLADDRTDMTGRSLAVSLALKTNRVFSYEASLLETSGNSVALCTDETAK
                     SELNAKNALLLDEAGYLAEAERRALKYYEDGEFALLFGEKDERRRGPIASLLHPKSQA
                     THREAEEQLRQLLLDESKRRVVLSALNRKKRDEV"
     gene            complement(2129..>2324)
                     /locus_tag="PLR57_04510"
     CDS             complement(2129..>2324)
                     /locus_tag="PLR57_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40749.1"
                     /translation="LPLALFWRNNTSCGSLVAVFPAGIVTFFAALRSNFLKSGWSWQT
                     DVQKRTAKPQYSAASAVFTF"
BASE COUNT          524 a          628 c          709 g          463 t
ORIGIN      
        1 ttaccgatac gctgcttcgc accctgctca aatgcagaaa ccgctgcccc gtcatgtttg
       61 ccagctccgc gcaggcggcg ctgccgaatc tttacggcag gagcaagaag gctggcgagg
      121 aactgctctt tcagtatcag cgggaaaccg acgcaaagat ctacgtgttc cgttttccgt
      181 ttgtctttgg caaatggtcg aaaccgaact acaacaaccc ggttgcaacc ttctgctaca
      241 acgtttccca cgatttgccg gttgtcgtga gcgatcccgc cgcgctgatc agccttgctt
      301 atgtggacga tcttgcggaa gcgctggtcg agctgattga cgaaacgccc gttgcaatgg
      361 acgggccgta ctgcgcagtt ccgctcgttt acacggcttc gctgggggac gttgcggatc
      421 agattcgctc gtttgccaag attcggacgg aaaacgtcct gccggatatg agttcgccgc
      481 tgaccagaaa gctatacgca acctacttga gctttttgcc aaccatggag tttgcgtatc
      541 cgctctttgc aaaagaggac gagcagggga tgtcggcaga gttcttgcgg ctctgtgcag
      601 gcgggcagat cagcgtttgc atcgtcaagc ccggcgcggt caaaggcaac cactggcatc
      661 acgcacgaac cgaaaaagtg cttgtggttt ccggcgccgg tgtgatacgc ttctgcagcc
      721 cgggcacgga cgacgtgatg gaatatcacg ttagcggcaa gaagttcgtt gtggtggata
      781 ttcccgttgg ttatgcccac aacctggaga acacgggcaa aacggacttg gtagcgctgc
      841 tttgggcgag cgaatggacg gatccggcgc gcccggacga cgttgattcg gtgctgccga
      901 tctactgagc aggctgaact caaaaaaaga accgacggcg cggaaaccag ttcttccgcg
      961 ctgttttctg caaaaaggga aacgacggaa cgttttccgt accgtttcca atgaaaggcg
     1021 aacacatggc aagaagcgaa tccggcatgc cggaacaaaa agcgataccg gaacccaaag
     1081 cgaatatcct gcttctggga ggcacaggcc cgctgaaaga tgcggccgcg cccgtgcttt
     1141 ctgaggatgt gaagcgcctg tttggtgcaa gcggcttccg ctgcggcgcg ctgttttgcg
     1201 acaggaatac gcatggcggg gcgggtgaaa cgggtcaaaa cgcaattgaa tggctcaaga
     1261 acacgggaat cggcctcgtt tcgctctgcg gcgtgctgca ggaggatttg cagggaacgc
     1321 tcgacgcgct ggacggggtt aagactctgg gcgttggagc gaatcaggcg ttggcgaacc
     1381 gcgcggaggt cgtttcggtc aacggcgtcc ggctaggatt tatcgcgctc aacgagtgct
     1441 cgggcgaccg ggaacgtttc gcgccggaag cggacattct ggaggcggac gcgctcgacc
     1501 gggtgcgcaa gctactgccg cagtgcgacc atatcatcgt attcagccgt cttggcattc
     1561 cgggctacgg ccttccgctg ccggaatggc gcgcgcgctg ccgcaggttt atcaaggcgg
     1621 gcgcgagcgt tgtcgccgtc atggagccgg atgcgctgat gggctgggaa gaatacgaaa
     1681 acggtctgat tctctacggc cttggcacgc tggccgacga ccgaacggac atgacaggca
     1741 gatcgcttgc cgtttcgctt gcgctcaaaa caaacagagt gttctcctat gaagcgagtt
     1801 tactcgaaac gagtggcaac agcgtcgctc tctgcaccga cgaaacggct aaaagcgagc
     1861 tgaacgcaaa gaacgcgctg cttctggacg aagcgggcta ccttgccgaa gcggaacggc
     1921 gcgcgctgaa gtattatgaa gacggcgagt ttgccctgct ctttggcgaa aaggacgagc
     1981 gcagacgcgg gccgattgcc tcgctgctgc acccaaaatc gcaggcgact caccgcgaag
     2041 cggaagagca gctccggcag ctgctgctgg acgagtcgaa aagacgggtc gtcctcagcg
     2101 cgctgaaccg gaagaaacgc gacgaagttt aaaaagtaaa aacggcagag gccgcgctat
     2161 attgcggctt cgccgttctt ttttggacgt ccgtttgcca tgaccatccg ctttttagaa
     2221 aatttgatct taaggcggca aaaaaagtca caatacctgc cggaaagaca gcaacaagac
     2281 tcccacaact tgtgttgtta cgccaaaaca acgcaagcgg tagt
//
LOCUS       DAOMGY010000240         2318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_51628_length_2318_cov_3.505524, whole genome shotgun sequence.
ACCESSION   DAOMGY010000240 DAOMGY010000000
VERSION     DAOMGY010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51628_length_2318_cov_3.505524"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLR57_04515"
     CDS             <1..519
                     /locus_tag="PLR57_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40750.1"
                     /translation="NPGNNTASDSAITLNNPTKDGYLFEGWTCAQLGITEPAPSVTIA
                     EGTTGALTFVANWVVNPAGGMGGGGGSGGGSSSTSAATQDDTTTSAAATPAPTTTDAG
                     TTSTRRVKTASSSTKVTFSSEVDVVEPTLESVRGNTFPWGWSLLGVGGAAVIGYAIAL
                     ISRKHRENVKTK"
     gene            complement(640..1071)
                     /locus_tag="PLR57_04520"
     CDS             complement(640..1071)
                     /locus_tag="PLR57_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40751.1"
                     /translation="MSEVKKNMDKMPKWAKYTLYGILGIAGAFLLGLLFGNVIMWLWN
                     WLMPKFFGLSTITFWEGLGLFLLAHILFGFGSSSHSDDSGKKHHKKNHRHGGEAEKKD
                     WKDWEYYDDWWEEDGKTAFHAYAERKKTTPDEQEPPKADAE"
     gene            complement(1135..1677)
                     /locus_tag="PLR57_04525"
     CDS             complement(1135..1677)
                     /locus_tag="PLR57_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQQ40752.1"
                     /translation="MEQDRASAWYEFFSLENGRLKNYVRKQAQRISDMDAEDIVAEVM
                     LHTVSRLGTSGPVENIAAYVYRSIRNRIADYERKRAKSTSLDGMLGEDGELPFLQALV
                     TENDDPFRAEEQAERIHRLTDAIGKLEPRQRAVLIATELKGKSFRELSEAWGEPVGTL
                     LSRKSRAVKALRALLEETEP"
     gene            complement(1880..>2318)
                     /locus_tag="PLR57_04530"
     CDS             complement(1880..>2318)
                     /locus_tag="PLR57_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40753.1"
                     /translation="ENSPCLLLSGGGVTLVNMKLSAKSVPLVYAPSGDSRLMLEGTAL
                     SAAPVIDEFATLWLELKNGSSLIGTFGESLPARASVSLDAASTWTLDADAYVTVFLNA
                     DTTNANIVSNGYSIYYDSNAPENEPLGGQSYALPGGGYLVPLI"
BASE COUNT          443 a          673 c          681 g          521 t
ORIGIN      
        1 aatcccggca acaatactgc gtcggactcc gccatcaccc tcaacaatcc gaccaaggac
       61 ggataccttt ttgaaggctg gacttgcgcg cagctcggca ttaccgaacc cgccccgagc
      121 gtgaccattg cggaaggcac gaccggcgca ttgacgttcg ttgccaactg ggttgtaaac
      181 cctgcgggcg gcatgggcgg cggaggcggt tccggcggcg gaagcagttc cacttcggcc
      241 gcaacgcagg acgatacaac aacaagcgcc gcggcaacgc ccgcgccaac cacaacagac
      301 gcgggcacga ccagcacgcg gcgggtcaag accgcttcct cttcgaccaa ggtgacgttc
      361 tccagcgaag tggacgttgt ggagcccacg ctcgagagcg tgcgcggcaa cacgttccct
      421 tggggatggt cgcttctggg cgttggcggc gcggcggtca ttggctacgc gattgccctg
      481 atcagccgca agcaccggga gaacgtgaaa acgaagtaac gccgggggtt tcacccccga
      541 acccctgacc aaagggcacg ccctttggaa acccactttt gatcgatcaa gaattaaaaa
      601 gcggatgcga taggcatccg cttttgtgct gctgttcggt tattccgcgt cggctttcgg
      661 gggttcctgt tcgtcgggcg tcgttttctt gcgctcggca taggcgtgga aggcggtctt
      721 tccgtcctct tcccaccagt cgtcgtaata ttcccagtct ttccagtcct tcttttcggc
      781 ctcgccgccg tggcggtggt ttttcttgtg gtgctttttg ccgctgtcat ccgaatggct
      841 ggaactgcca aagccgaaca ggatgtgcgc gagcaggaag aggccgaggc cttcccagaa
      901 ggtgatcgtg gaaagtccga agaacttcgg catcagccag ttccagagcc acatgatcac
      961 gttgccaaag agcagaccca gcaggaacgc tccggcgatt cccagaatgc catacagcgt
     1021 atacttcgcc cacttgggca tcttgtccat atttttcttt acttcgctca ttttattttc
     1081 tcctttgccg gagatgcgta cccggcgttt ctttgttaaa ttgttgtgtt ggcgttacgg
     1141 ttccgtttcc tccagcagcg cgcgcagggc tttgaccgcg cggcttttgc gggagagcag
     1201 cgtgccgacg ggttcgcccc aggcctcgct gagctcgcgg aaggactttc ccttgagctc
     1261 ggttgcaatc aacaccgccc gctgtcttgg ttcgagcttg ccgatggcgt ccgtcaggcg
     1321 gtggattcgc tcggcctgtt cttccgcccg aaacgggtcg tcgttttcgg taacgagcgc
     1381 ctgcaggaaa ggcagttcgc cgtcctcgcc cagcatgccg tccagactcg tggattttgc
     1441 gcgtttgcgc tcgtaatcgg caatgcggtt gcggatggag cggtagacgt aagcggcgat
     1501 gttttcgacc ggtccggatg tgccgaggcg ggaaacggta tgcagcatga cctcggcgac
     1561 gatatcctcc gcgtccatgt cgctgatgcg ctgtgcctgt tttcgcacgt agttcttcag
     1621 ccgcccgttt tcaaggctga aaaactcgta ccatgcgctt gccctgtcct gttccatcgt
     1681 ccgctcctgt cccatacact cttaagacgc gcgaagaacc cgtttattgc atgttttcta
     1741 aaaaaagttt gtacggataa tggatatata tcatatttgt ccgtttctgc ccagcttagg
     1801 gggagaattc gcgtgaaaaa aagagcacgc gccgtttaga cgcatgctca gattgaaatc
     1861 gataagggtt atagcttcgt tagatcagcg gaaccagata cccgccgccg ggcagcgcgt
     1921 aggactggcc gccgaggggt tcgttctcag gcgcgttgga atcgtaataa atcgaatacc
     1981 cgttggagac gatgttggca ttcgtcgtgt ccgcgttgag aaaaacggtt acgtacgcgt
     2041 ctgcgtccag cgtccaggtg ctggctgcgt ccagcgaaac gctcgcccgc gcgggcaggg
     2101 attccccgaa cgttccaatc agcgaggaac cgttcttgag ctccagccag agggtggcaa
     2161 attcgtcgat tacgggagcc gcggaaagcg ccgtgccttc cagcatcagc cggctgtcac
     2221 cgcttggtgc gtacacgagc ggaacgctct ttgccgagag cttcatgttg accagtgtaa
     2281 cgccgccgcc ggaaagcagc aggcagggcg agttttca
//
LOCUS       DAOMGY010000241         2315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_51745_length_2315_cov_2.582301, whole genome shotgun sequence.
ACCESSION   DAOMGY010000241 DAOMGY010000000
VERSION     DAOMGY010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51745_length_2315_cov_2.582301"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /gene="recF"
                     /locus_tag="PLR57_04535"
     CDS             complement(<1..1048)
                     /gene="recF"
                     /locus_tag="PLR57_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HQQ40754.1"
                     /translation="MRVTGLRLVNFRNYPSLSLRPDAGLCVLTGENAAGKTNVLEALF
                     LCSLGRSHRTVRDSEMVRKGESAGSVAVDMDTRSGPRRIECKLFSGDRKKMFIDGAPL
                     SRSGELLGCLNVVMFAPEDLALIKDGPGERRRFLDMAISQLRPSYYYTLQQYNAALKQ
                     RNALLKEPEGLDYDALECWDEQLSVLGAAIVLARAELVEHMGQIAAEQHETLSDGAET
                     LVAAYEPNVEPSDRESLAKTLRLALADTAERDVYRGSTSVGPHRDDLKLVINGSDVRV
                     YGSQGQQRTTVLSLKLAQLEILRAEKEDSPVLLLDDVFSELDRNRQKMLLIAAQDCQT
                     FLTCTHIEEVAAAGV"
     gene            complement(1448..2140)
                     /locus_tag="PLR57_04540"
     CDS             complement(1448..2140)
                     /locus_tag="PLR57_04540"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HQQ40755.1"
                     /translation="MNPILRAETDPLATAFNIKELFSSTLNPLQVILTLIIALAIGGF
                     LYFIYKKTFAGVVYSRSFNLSLILLTMVSAMVIMLISSNLTLSLGMVGALSIVRFRTA
                     IKDPIDTVYMFWAVGEGLALGAGFVDVGLIGALVIGVIMVIITSAKGNASMPYLLILH
                     FDDRASQQIKGLIRQLPKAHVKSKTVQHDGIELTIELRIRESDTDFVDKFLRVPGVFD
                     ATLVAHQGDLLS"
BASE COUNT          515 a          654 c          660 g          486 t
ORIGIN      
        1 ccacgcccgc ggcggcgact tcctcaatat gggtgcaggt caaaaacgtc tggcagtcct
       61 gcgctgcaat taacagcatc ttctggcggt tgcggtcaag ctcggaaaac acgtcgtcca
      121 gcagcagaac aggcgaatcc tccttttccg cgcggagaat ttcgagctgc gccagcttca
      181 ggctcaaaac agtcgtacgc tgctgtccct gggagccgta cacgcgcacg tccgaaccgt
      241 tgattacgag cttgagatcg tccctgtgcg ggcccacgga ggtgctgccg cggtagacgt
      301 cccgttcggc ggtgtccgca agcgcaagac gaagggtctt tgccagactc tcgcggtcgg
      361 aaggctcgac gttcggctcg taggccgcaa ccagcgtttc cgcgccgtcc gagagggttt
      421 cgtgctgttc cgcggcgatt tgccccatgt gttctaccag ttccgcacgc gccagcacga
      481 ttgccgcgcc cagcacggag agctgttcgt cccagcattc cagcgcatcg tagtccagac
      541 cttccggctc cttgaggagc gcgttgcgct gtttgagcgc ggcgttgtat tgctgcaggg
      601 tgtaatagta cgaaggccgc agctgcgaga ttgccatatc gagaaaccgc cgccgttcgc
      661 cggggccgtc cttgatgagc gcgaggtctt ccggcgcgaa cattacaacg ttcagacagc
      721 cgagcagttc gccggagcgg gaaagcggcg cgccgtcgat gaacatcttc ttgcggtcgc
      781 cggaaaagag cttgcactcg attctgcgcg ggccggaacg cgtgtccata tccacggcta
      841 cgctgccggc ggactcgcct ttacgtacca tttcgctgtc ccgcacggtg cggtggctgc
      901 gcccgagaga gcataaaaag agcgcctcca gcacgttggt tttccctgcg gcgttctccc
      961 ccgtcagtac gcaaagaccc gcgtccggac gcagggaaag cgacgggtaa tttctgaagt
     1021 tcacgagccg taggccggta acgcgcatgc ggacgctgcc caatcttcct tgtaaattct
     1081 tattgtatta taccatgttt agaaggttct gtatatatat tatagaaaag agatggcatt
     1141 tttggtgaat ttggccgtgt ttttacgcag tatttccatg tttgccgcat tcccgcgaca
     1201 tgcttaaaaa agaaccatga accttcgctg tttttcgaca gagcggttag gttcgataaa
     1261 gaaaaaatcc gccctattcg ggcggaacaa gccgttcatt cgggtaagag aaagccgggc
     1321 ggcgctgagc tcagcccgtc cggcatcatc attaccgaat gaattttacg agagcaagtc
     1381 tctctgatgc gcaaacagcg ttgcgtcaaa acgccaatct atttttcaaa aatagattaa
     1441 aaagatttta cgagagcaag tctccctgat gcgcaaccaa cgttgcgtcg aacacgccgg
     1501 gcactctgag gaacttatcc acaaagtccg tgtcgctttc acggatgcga agctctatgg
     1561 tcagctcgat gccgtcgtgc tggacggtct tgctcttgac gtgcgccttg ggcagctggc
     1621 ggatcaagcc cttgatctgc tggctcgcgc gatcgtcaaa gtgcagaatc agcaggtacg
     1681 gcatgctcgc gttgcccttg gcggacgtga tgatgaccat aatcacgccg atgaccagcg
     1741 cgccgatgag gccaacgtcc acaaaccccg cgccgagcgc aaggccttcg cccacggccc
     1801 agaacatata caccgtgtcg atggggtcct tgatggccgt gcggaaacga acgatggaca
     1861 gcgcgccgac catgcccaga gagagcgtca ggttcgagct gatgagcatg atgaccattg
     1921 ccgaaaccat ggtcaagagg atcaggctga ggttgaacga gcgcgaatac acgacgcccg
     1981 caaacgtctt tttgtagatg aaatacagga atccaccgat cgcaagagcg ataatcagcg
     2041 tcaggataac ctgcagagga ttcagagtgc tggaaaacag ctctttgata ttaaaggcgg
     2101 ttgcaagcgg atccgtctcc gcgcgcagaa tggggttcat atggttcctc cttcacacat
     2161 tgctggtgat aaactagtat accgcgccgc gcgcgaaaaa caaagcaaaa atccagccgc
     2221 agcgcggtaa accggcgctt gggctaaaac tcgtactgcc tgcaaaagat atatttgctg
     2281 gcggcggtgt gctccgccga gccgacggtc agcag
//
LOCUS       DAOMGY010000242         2306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_52057_length_2306_cov_3.131053, whole genome shotgun sequence.
ACCESSION   DAOMGY010000242 DAOMGY010000000
VERSION     DAOMGY010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52057_length_2306_cov_3.131053"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLR57_04545"
     CDS             complement(<1..402)
                     /locus_tag="PLR57_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ40756.1"
                     /translation="MKQTCTICPRKCVLEEGQTGFCRARVNLGGKVICGNYARVTALW
                     LDPIEKKPLKRFFPGTRILSVGSYGCNLACPFCQNSDISMANEKSVDWRIISPEELVA
                     QAEQLKSAGNIGIAFTYNEPMINLEYMRDTFR"
     gene            complement(446..1831)
                     /gene="amrA"
                     /locus_tag="PLR57_04550"
     CDS             complement(446..1831)
                     /gene="amrA"
                     /locus_tag="PLR57_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HQQ40757.1"
                     /translation="MPVLMACIVPHPPIILPEIGKGQEWEIRSTTAAYKEIARRVREK
                     KPETIVVLSPHATSYADYFHISPGTKARGDFSRFGAKQVEVTAYYDEEFCKALEVTAK
                     RAGIPAGTQGERDKALDHGTMIPLTFLAEKELPCAIVRIGLSGMDAVAHYRLGKCIKK
                     VSDDLNRDVVIIASGDLSHKLLESGPYGYAEEGPVFDKAVTKAMAEGDFFAMMTFDPH
                     LTEAAAECGLGSFEIMAGAFDRMSVKSELLSYEGPFGVGYAVAAFEPAEADESRAFDV
                     RSQKVERERISTIRDQEDAYTRLARQTVESFVKTGQVPPLPDEMPEEMKNTRAGVFVS
                     LKIQSKLRGCIGTISAVTGSVAEEIQRNAVSACSQDPRFDPVTADELERIHYSVDVLG
                     KAERIESVSQLDAKRYGVIVTSGYRKGLLLPNLEGVDTVEEQLKIAKQKAGIPESERC
                     RMERFEVVRHT"
BASE COUNT          465 a          664 c          607 g          570 t
ORIGIN      
        1 ccggaaggta tcccgcatgt attcaagatt gatcatcggc tcgttgtagg taaacgcaat
       61 tccgatattg cctgcgcttt tgagctgttc cgcctgcgcg acgagttctt cgggagaaat
      121 aatcctccag tcaaccgact tttcattcgc catggagatg tcgctgttct ggcaaaacgg
      181 gcaggcgaga ttgcagccgt agctgccgac ggataggatg cgcgttcccg gaaagaagcg
      241 cttgagcggt tttttctcga tcgggtcgag ccacagcgcg gtcacgcgcg catagttgcc
      301 gcagatcacc ttgccgccaa gattcaccct tgcgcggcaa aagccggttt gcccttcctc
      361 cagaacgcat tttcgcgggc agatcgtaca cgtctgtttc ataagcgccg aaccacctta
      421 ctgaaaaaat gtgaacccgt cgtaattatg tatgccggac gacttcgaag cgctccatgc
      481 ggcagcgctc gctttccgga atgcccgcct tttgttttgc gattttaagt tgctcttcca
      541 cggtatcgac cccttcaagg ttgggcaaaa gcaaaccttt gcggtagccg gaagtcacga
      601 tgaccccgta ccgctttgcg tcgagctggc tgacgctctc aatgcgctcg gctttcccca
      661 gcacatccac gctgtagtga atccgctcca gttcgtccgc cgtcacgggg tcgaatcttg
      721 gatcctggct gcaggcggaa accgcgttgc gctgaatctc ctcggcaacg ctccccgtca
      781 ccgcgctgat ggtgccgatg cagccgcgga gcttactctg aatcttgagc gaaacaaaaa
      841 cgccagcacg tgtatttttc atttcttccg gcatctcgtc cggaagcgga ggaacctgcc
      901 cggtttttac gaatgattcc acggtctggc gcgcaaggcg cgtatacgca tcctcctgat
      961 cgcgaatcgt ggaaattcgt tcgcgttcca ctttttggct gcgcacgtcg aacgcgcgcg
     1021 attcgtccgc ttccgcaggt tcgaacgcgg caactgcgta gcctacgccg aacgggcctt
     1081 cgtaggagag cagttcgctt tttacgctca ttctgtcgaa cgcgcccgcc atgatttcaa
     1141 acgagccgag cccgcattcc gcggcagcct ccgtcagatg ggggtcaaac gtcatcatgg
     1201 caaaaaagtc gccttccgcc atcgccttgg taacagcttt atcgaacaca ggcccttcct
     1261 cggcatagcc gtacgggccg ctttcgagca gtttatgcga gaggtcgccg ctggcgatga
     1321 tcacaacgtc cctgttgaga tcgtcggaca cttttttgat gcacttgccg agccggtaat
     1381 gcgccaccgc atccatgccg gaaagcccga tgcgcacgat ggcgcagggc agttcctttt
     1441 ccgccagaaa cgtcagcgga atcatcgtgc cgtggtcgag tgctttgtcg cgttcgccct
     1501 gcgtacccgc gggaatgccc gcgcgctttg ccgtaacttc cagcgctttg cagaattcct
     1561 cgtcgtaata ggcggtcact tccacctgct ttgcgccgaa ccggctaaaa tcgccgcgcg
     1621 ctttcgtacc cggggagata tgaaaatagt ccgcatagct tgttgcgtgc ggagacagaa
     1681 cgacgattgt ttcgggtttc ttttcgcgga ctctgcgggc gatttcctta tatgcggcgg
     1741 tcgtgctgcg gatttcccac tcctgccctt ttccaatttc cggcaggata atcggcggat
     1801 gcggcacaat acaagccatc agtacgggca tagatatccc tctcttttga atcatcgtat
     1861 tttgctggtt ccggttgcgg ttctcagcat aggccgcgca gggcgcgcgg cggacaggca
     1921 cttgatcgaa catattgtac cattgtgcgc attccctgtc aaagttccgc aacgcgggcg
     1981 tatggttctg ttgaaacccg tctccagcaa gcaagttata ttcttcttcc cgctatatac
     2041 tggattcgtt cacagatatt ctacttgcgt tcatcataat ttacggtgca tctgtgttaa
     2101 aatgaagtaa gatttttccc tgcatccgca gaggcacctc tttggcgttg atcaaaaatc
     2161 gtctgctgtc aggaaactac ggacaatatg gagccagtaa aaaccatcat gacggaactc
     2221 gaaaaacgaa aaagaagtaa gatcgatatg cgtacggctg ccgttctggg gctcggcagc
     2281 gtctgcttgc tttgcatgct gtttct
//
LOCUS       DAOMGY010000243         2294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_52441_length_2294_cov_3.185351, whole genome shotgun sequence.
ACCESSION   DAOMGY010000243 DAOMGY010000000
VERSION     DAOMGY010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52441_length_2294_cov_3.185351"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="PLR57_04555"
     CDS             <1..1397
                     /locus_tag="PLR57_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40758.1"
                     /translation="SVTVNMKTADGRAYTAGVWTNQTVTVTAVGADDLNPVTFQYAQD
                     GAAYAAAENSVVTTGVHSVYILATDAFDNAAEFGPYQARVDKQPPAVPKIAESLSGAN
                     IVLTLTFTADPGGSGNDYLILPDGTRAAAKGTPEFAVSRNGTVSFTLYDAAGNKTTFS
                     YTVSSVDTSKPVITCSSGGYSIGSTTQSSISASLSFTDNESELTARGYQLSTSAVPGG
                     SYKNYGGSISITAPGTYYIHAYAKNAFGLTTYETFGPFIVEAVPQATPAPTTIEETGD
                     VVVDVEDIADDVPQPPVYIRLPGEEWSQTLTLEDVGPGDYLVEAMDSEGNIYTTTVHV
                     TMRDIVARSLRNSGTSWGIAAIAGELGLAALILILLFAGYNVTVTVFGESLGETKKLR
                     ALRRILFRRDKLIIKLENKHVSGGEYANIHVAKHLTKRMRKRTIVITVRGQEVLSEYI
                     PEDINEAFQRKIEL"
     gene            complement(1538..>2294)
                     /locus_tag="PLR57_04560"
     CDS             complement(1538..>2294)
                     /locus_tag="PLR57_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40759.1"
                     /translation="FYLTGRNEIYIYFTSLLGGEGVVESISERLAESVGAAEAERVLE
                     GLEHPPLGSEPADFPAFTKALMERLESNLPEETVQCVLAGNNHGIPAAAFEEAKALFS
                     ASASMDEFLLAYHKKQVAELQHHCDTNTVWYEQSITQEVVDFVSANQEIQSAVREGDV
                     LYTTKIPYDPAQYLAETDPVKKRYYACHCPFVREAILAGSPAVSENWCYCSGGFVKYP
                     YEVILGRPLRVKMLQSVLRGDPVCRFAIDIAEA"
BASE COUNT          523 a          639 c          674 g          458 t
ORIGIN      
        1 tgagcgtaac ggtgaacatg aagacggcgg acggacgcgc ttatacggcg ggcgtctgga
       61 caaatcagac cgtgaccgtt acggcggtcg gtgcggacga cctgaacccg gtcaccttcc
      121 agtatgcgca ggatggagca gcctatgcgg cagccgagaa ctcagtggtc accacgggcg
      181 tccacagcgt gtatatcctt gcaacggacg cgttcgacaa cgcggcggaa ttcgggccgt
      241 atcaggcgcg cgttgataag cagccgcctg ccgtgcccaa gattgccgag agcctctccg
      301 gcgcgaatat cgtgctgacg ctgacgttta ctgccgatcc gggcggaagc ggaaacgact
      361 acctgattct gccggacgga acacgcgcgg cggcgaaagg aaccccggag tttgccgttt
      421 ccagaaacgg aacggtctcg tttacgctct acgacgcggc gggcaacaag acgacgttca
      481 gctataccgt ttcgagcgta gatacctcca agccggtgat cacctgctcc agcggcggtt
      541 acagcattgg cagcacgacg cagagcagca tctccgcttc gctgagcttt acggacaacg
      601 aatccgaact cacggcgcgc ggttaccagc tctcgacgag cgcggtgccg ggcggatcgt
      661 ataagaacta cggcggttcc atttcgatta ccgcgccggg aacttactac atccacgcct
      721 acgccaagaa tgcgttcggg ttaacgacgt atgaaacgtt cggcccgttc atcgtagaag
      781 cggttccaca agccacaccc gcgcccacaa ccatcgaaga aaccggcgat gtggtggtgg
      841 atgttgagga catcgcggac gacgttccgc agcccccggt ctatatccgc ctgccgggtg
      901 aagaatggtc gcagacgctg acgctggaag acgtcggccc cggcgattac ctcgtggaag
      961 cgatggattc agaaggcaac atttacacaa cgacggtgca tgtgaccatg cgcgatatcg
     1021 ttgcccgttc gctgcgtaac agcggaacaa gttggggcat tgcggcgatt gcgggcgagc
     1081 tcggactagc ggcactcata ctgattctgc tgtttgccgg gtacaatgta accgtaacgg
     1141 tatttggcga atcgcttggt gaaacgaaga aactccgcgc actgcggcgc atcctgttca
     1201 gaagggataa gctgatcatc aagctggaga acaaacacgt ttccggcggt gaatacgcga
     1261 atatccacgt tgccaagcat ctgacaaagc gcatgcgcaa gagaaccatc gtaatcaccg
     1321 tgcgcggcca ggaagtgctg agcgagtata tccctgagga tatcaacgaa gcgttccagc
     1381 ggaagatcga gctgtaaaga caaacgcaaa aagaagagcg gggcgttgac agcgccccgc
     1441 tctattttgt aaaatcagcc ggaatacgtt tgattattgc gcattgtgaa acctgacgcg
     1501 gattttactt tgttgagtaa aatttgagaa ttcggtgcta cgcttcggct atatcgatgg
     1561 caaacctgca aacggggtct ccgcgcagca cggactgcag catttttacg cgcagtggcc
     1621 gaccgagaat cacttcgtac gggtatttca caaatccgcc gctgcagtag caccagtttt
     1681 ccgatacggc gggggaaccc gccagaatcg cttcgcgcac gaacgggcag tggcaggcgt
     1741 agtaccgctt ctttactggg tcggtttccg ctaagtactg tgccggatcg tatggtatct
     1801 tcgtggtgta aagcacgtcg ccttcccgca cggcggactg aatctcctga ttggcggata
     1861 caaaatccac gacttcctgc gtaatcgact gttcgtacca gaccgtgttg gtgtcgcagt
     1921 ggtgctggag ttcggcaacc tgctttttat gatacgccag caggaattca tccatggaag
     1981 cggaggcgga aaacagcgct tttgcctcct caaacgcggc ggcggggatg ccgtggttat
     2041 tgcccgcaag cacgcactgc accgtctctt cgggaaggtt cgactccaga cgctccatca
     2101 gagccttggt gaaagcggga aagtccgcgg gctcgctgcc gagcggcgga tgctccagac
     2161 cttcgagaac gcgctctgct tcggcagcgc caacggattc ggccagacgc tccgaaatac
     2221 tttccacaac gccttctcct ccgagcaggg acgtaaagta gatgtagatt tcgtttctgc
     2281 cggtcagata aaag
//
LOCUS       DAOMGY010000244         2293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_52485_length_2293_cov_2.304736, whole genome shotgun sequence.
ACCESSION   DAOMGY010000244 DAOMGY010000000
VERSION     DAOMGY010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52485_length_2293_cov_2.304736"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..708
                     /locus_tag="PLR57_04565"
     CDS             70..708
                     /locus_tag="PLR57_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40760.1"
                     /translation="MASFGSLKHFTAENAPEGSAERCLDCSVAATCPYSATRLYMIPF
                     LTGWPVSVITDDISPAGRIKALREGPYGRCVYHCDNDVVDHQSTILEFEGGVTATMTV
                     SAFTDFRHGRTIHIMGSHGEIRAKMSEETIEVTNFRTGSVRSTENPLPGGMSDGHGGG
                     DMGLIGAFVRMELGEEGIVKSSIHDSIQSHLICIAAERSRHEHTVVDVDSKF"
     gene            788..1252
                     /locus_tag="PLR57_04570"
     CDS             788..1252
                     /locus_tag="PLR57_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40761.1"
                     /translation="MTTSKTRTRAETFFLIISCAYLIIAAAMIGLALYRLTGENVTLS
                     IGNVTDAVFAIIGGVLGFVAGVFGLVSKKMKRCRLMGLALLLIAAVPLAINLLAGKTF
                     AVCWKNILLMVMPLLYLIGSLLKRSAKPASAAPQTQPVQPEKPAEATQKTND"
     gene            complement(1357..>2293)
                     /locus_tag="PLR57_04575"
     CDS             complement(1357..>2293)
                     /locus_tag="PLR57_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQQ40762.1"
                     /translation="ETLFAVTTELKMRYALDLPTEWLKNKSLFMLFFEESTRTRNAFE
                     CGITQLGGHANYLTPKMTQIDHGETPKDTIEVLSRMGHGIGVRNTFVNGHKWMEAIAE
                     FSKIPVFNMQCDIWHPTQSFADLYTIKEKFHNELKGRKFVISWTSAGKYMRPLSMAQS
                     LVALMPRFGMDVTLAHPKGFELMPEPIRLARENAARYGSKFEVVDDMDEAVRGADVVY
                     AKGWGPVMTVGDDEAAGVEMINQNPGWIVDKRRMDLAGPDSIYMHCMPADRDIEVTSE
                     VMDGPHSVLYDEAENRLHTIKALMALTMGNSLARR"
BASE COUNT          465 a          640 c          698 g          490 t
ORIGIN      
        1 atgttcctcg ctaagagctg tcatgatacg gatatcctct tgtacctgat cggttaggac
       61 tgcaagcgcg tggcgtcgtt cggttccctc aagcacttca cagcggaaaa tgcgccggaa
      121 ggcagcgcgg aacgctgttt ggactgttcc gttgccgcaa cgtgccccta cagcgcgacc
      181 aggctctata tgattccgtt tctcaccggc tggccggttt cagtcattac ggacgatatc
      241 agccctgccg gacgcatcaa ggcgctgcgc gaagggcctt atggccggtg cgtctaccac
      301 tgcgacaacg acgtggtcga tcaccagtcc accattctgg agttcgaagg cggcgtaacc
      361 gctaccatga cggtttccgc gttcacggac ttccgccacg gacgcacgat tcacatcatg
      421 ggctcccacg gcgaaatccg cgccaagatg agcgaggaaa ccatcgaagt tacgaacttc
      481 cgcaccggtt ccgtccgctc gacagaaaat ccgctgcccg gcggcatgag cgacggtcac
      541 ggcggcgggg acatgggttt gatcggcgca ttcgtgcgca tggaactcgg cgaggaaggc
      601 attgtcaaga gttccatcca cgactcaatc cagagccacc tgatctgcat cgctgcggag
      661 cgttcgcggc acgagcacac ggttgtagac gtggacagca agttctagtt tgaaagagag
      721 accgctcaac agcgcaaact attttggtaa cgcagaaagc gaattacagc agaaagggcg
      781 gagcagtatg acgacttcga aaacacgcac gcgcgcggag accttctttc tgattatcag
      841 ctgcgcgtat cttattatcg ctgcggcgat gatcgggctc gcgctttatc ggttaacggg
      901 ggagaatgtt acgctctcca taggcaacgt aaccgatgcg gtgttcgcca tcatcggcgg
      961 cgtgctcggc tttgttgcgg gcgtattcgg gctcgtcagc aagaagatga agcgctgccg
     1021 cctgatgggg ctggcgctgc tgttgatcgc ggcggttccg ctggccatca acctgcttgc
     1081 gggcaagacg ttcgcggtct gctggaagaa catcctgctc atggtgatgc cgctgctgta
     1141 tctgattggt tcgctgctta aacgcagcgc aaagcctgct tcagccgcgc cgcagacgca
     1201 gccggttcag ccggagaagc ccgcggaagc aacacaaaaa acgaacgact agccgccagc
     1261 tactcacaaa gcaacagaaa aagcccggaa ttttgcttcc gggccttttt gcatgtattt
     1321 tgcttgctgc aaggggaagg gaatagcgcg tttgcgttat ctcctagcca gcgagttgcc
     1381 catggtcagc gccatcagag ccttgatcgt gtgcaggcgg ttttccgctt cgtcgtacag
     1441 aacggaatgc gggccgtcca tgacttcgct cgtgacctcg atgtcgcgat cagcgggcat
     1501 gcagtgcatg tagatcgagt cggggccggc aagatccatc ctgcgcttgt ccacgatcca
     1561 gccggggttc tggttgatca tttcaacgcc ggcggcttcg tcgtcgccaa cggtcatgac
     1621 cgggccccag ccctttgcgt agacgacgtc cgcgccgcga acggcttcgt ccatatcgtc
     1681 cacaacctca aacttggagc cgtagcgcgc cgcgttttca cgcgcaagac gtatgggttc
     1741 cggcatgagc tcaaagccct tggggtgcgc cagcgtaaca tccatgccaa agcgcggcat
     1801 gagggcgacc agcgactgcg ccatggagag ggggcgcatg tatttgccgg cggacgtcca
     1861 ggagatgacg aacttgcggc ctttcagctc gttatgaaat ttttccttga tcgtgtagag
     1921 gtcggcgaac gactgggtcg ggtgccagat gtcgcactgc atgttgaaga caggaatctt
     1981 ggagaactcg gcgatggctt ccatccactt gtgtccgttg acgaacgtgt tgcgaacgcc
     2041 gatgccgtga cccatgcggg agaggacttc gatggtatcc ttcggggttt cgccgtggtc
     2101 gatctgggtc atcttggggg tgaggtagtt cgcgtggccg ccgagctgcg taataccgca
     2161 ttcgaaggcg ttacgggtgc gggtggactc ttcaaagaac agcatgaaca gcgacttgtt
     2221 cttgagccat tcggtgggca ggtcgagcgc gtaacgcatt ttcagctcgg tggtgaccgc
     2281 aaagagggtt tca
//
LOCUS       DAOMGY010000245         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_52616_length_2289_cov_3.431065, whole genome shotgun sequence.
ACCESSION   DAOMGY010000245 DAOMGY010000000
VERSION     DAOMGY010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52616_length_2289_cov_3.431065"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="PLR57_04580"
     CDS             complement(<1..1464)
                     /locus_tag="PLR57_04580"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQQ40763.1"
                     /translation="MKEEDTVSHKYVYLFKEGSADMRALLGGKGANLAEMSNLGLPVP
                     FGFTVSTEACTRYYADGKTIAQEIVDQIEAALAIVEKEAGKKFGDPSNPFLVSVRSGA
                     RASMPGMMDTILNLGLNDETVIGLAKLTNNERFAFDSYRRFIQMFSDVVMEVKKSKFD
                     EIFEDVKHKNGFTLDTELTAENLKEIVIRYKKLYLAEKGTEFPQDPRVQLMEAVKAVF
                     RSWDNERAIIYRRMNDIPSDWGTAVNVQSMVFGNMGNDCGTGVAFTRNPSTGERKLYG
                     EFLMNAQGEDVVAGIRTPSPIDELKDSQPDVYQQFVTIAETLERHYRDMQDMEFTIER
                     GRLFMLQTRNGKRTAAAALQIAVDLVSEGMLTKEEALAKIDPKSLDALLHPTFEPKAL
                     KNACPIASGLPASPGAACGRIYFTAADASEASKRGEKVVLVRLETSPEDIEGMYASQG
                     ILTARGGMTSHAAVVARGMGTCCVAGCSEITVNEEKRT"
     gene            complement(1956..>2289)
                     /locus_tag="PLR57_04585"
     CDS             complement(1956..>2289)
                     /locus_tag="PLR57_04585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQQ40764.1"
                     /translation="DPGMENEAYADGYLVGTGANFVESFLTASLALENDGDVSAPVQS
                     DYGWHIIKRVSTEPAHEIAYEDIKESANAYFEAAYLTQYYQDIVDGWVADETLVTRYP
                     ENYASVGR"
BASE COUNT          500 a          620 c          629 g          540 t
ORIGIN      
        1 cgtgcgtttt tcttcgttca cggtgatctc ggagcagccc gccacgcagc aggtgcccat
       61 gccgcgcgca acgaccgccg cgtgggaggt cattccgccg cgggcggtca ggatgccctg
      121 agaggcatac atgccttcga tatcctcagg agaggtttcg aggcggacaa ggacgacctt
      181 ttcgccgcgc ttggacgctt ccgaagcgtc cgccgcggtg aagtaaatgc gtccgcaggc
      241 agcgcccggc gaagcgggca ggccggatgc gatcgggcaa gcgttcttca gcgccttggg
      301 ctcaaaggtg ggatgcagca gcgcgtccag cgacttgggg tcgatcttgg cgagcgcttc
      361 ttccttggtg agcatgcctt cgcttacgag atcgacggca atctgcaggg cggcggcggc
      421 ggtgcgcttg ccgtttctag tctgcagcat gaacaggcgg ccgcgctcaa tggtgaattc
      481 catatcctgc atgtcgcggt aatgccgttc aagcgtttct gcaatggtga cgaactgctg
      541 gtagacgtct ggctgggaat ccttgagctc gtcgatgggg cttggcgtac gaataccggc
      601 aacgacgtcc tcgccctgcg cgttcatgag gaactcgccg tagagcttgc gttcgccggt
      661 ggaaggattg cgcgtaaacg caacacccgt gccgcagtcg ttgcccatgt tgccgaacac
      721 catgctctgt acgttgacgg cggtacccca gtccgagggg atgtcgttca tgcggcggta
      781 gataattgcg cgctcgttgt cccagctgcg gaacacggcc ttgacggctt ccataagctg
      841 tacgcgcgga tcctgcggga attccgtgcc tttttctgcg agatagagtt ttttatagcg
      901 aattacgatt tctttgagat tttcggcagt gagttcggta tctaatgtga atccgttttt
      961 gtgtttgacg tcttcgaaga tctcgtcgaa cttcgatttt ttcacttcca ttacgacgtc
     1021 cgagaacatc tggataaagc ggcggtagct gtcgaacgcg aaacgctcgt tattggtcag
     1081 tttggcaaga ccgatcaccg tttcgtcgtt caggccaagg tttaaaatag tgtccatcat
     1141 gcccggcatg gacgcgcgcg cgccgctgcg gacggatacg aggaacgggt tggacggatc
     1201 gccgaatttc ttccctgctt ctttttcgac aatcgccaga gcggcttcga tctggtctac
     1261 gatttcctgc gcaatcgtct tgccgtccgc gtaataacgc gtgcaggctt ccgtagaaac
     1321 ggtaaagccg aagggaaccg gcaaaccaag attggacatc tcggccaggt tcgcgccttt
     1381 gccgcccagg agcgctcgca tatccgcgct gccttccttg aaaagataaa cgtacttatg
     1441 agacactgtg tcttcctcct tcatcattct aacaactaat tattatatcg ttggagccat
     1501 atgtatgcaa gaaaaatcgc aaacaggaac accgatcttc cgaatatgca tttttatatg
     1561 tccgtttgtt cactttatat agtaaaaacg cccgaaaacc agccggattg atggtttaag
     1621 gcgttttgtt atgcaatggc tcaaaatgca ggttctcctg cataaaatgc aatatttgcg
     1681 cgtagaattt cacgcaaatt tttcgttgtt aacaagtcgg ggcttccaca aggctgaata
     1741 tcatgttaaa acgcaaccag cgtacaaaaa aacggcagtt ttccattgcc ggaaatgcaa
     1801 aaaaaagcga ggccggaaaa ccggccccgc aaatcaaagc gcagggattt acagcacgga
     1861 gacaaagatc gaaatgaccg tgccaagcag catcattgcc gcaatcacca ggcagaggat
     1921 tctaacgcct ttgctcatct gtcaaaccct ccccatcagc gcccgaccga cgcgtagttt
     1981 tccgggtaac gggtgaccag cgtttcgtcc gctacccagc cgtccacgat atcctgataa
     2041 tactgcgtca gatacgccgc ctcgaaatac gcgtttgcgg attcctttat gtcttcgtaa
     2101 gcaatctcat gcgcgggttc cgtggaaacg cgcttgatga tatgccagcc gtagtcggac
     2161 tgcacaggag cggaaacgtc cccgtcgttc tccagcgcga gagacgcggt gaggaacgat
     2221 tccacaaagt ttgcgcccgt accgacgaga taaccgtcgg cgtaggcttc gttttccatg
     2281 cccggatcg
//
LOCUS       DAOMGY010000246         2283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_52811_length_2283_cov_4.623878, whole genome shotgun sequence.
ACCESSION   DAOMGY010000246 DAOMGY010000000
VERSION     DAOMGY010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52811_length_2283_cov_4.623878"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLR57_04590"
     CDS             <1..210
                     /locus_tag="PLR57_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HQQ40765.1"
                     /translation="PRGDMYEIGLWSDSIVETGDEIMYAINIPQESVTIPETIDGIRA
                     ACGMQQTRETATDATNEYLGIGRFK"
     gene            339..1604
                     /locus_tag="PLR57_04595"
     CDS             339..1604
                     /locus_tag="PLR57_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQQ40766.1"
                     /translation="MTRFGIHTMDAYDVQGKTVLCRVDINQPVDRVSGKLKSVNRIRA
                     CVPTVRELADRGAKVVLLAHQGSDIEYKNFYTTAPHAEVLSELLEREVKFIDDVTGPA
                     ARQAIRSLKNGEVLLLDNVRFCSEEQTLFETKLCLTHEQQAKTQVVEKLAPLADLYVC
                     DAFAAAHRDQPSLCGFEQVLPSMMGRLFEQEFCVISGIMEAPERPCVFVLGGAKVADA
                     FLMMETVLSGGTADKVLTGGLVANILLAAKGEQIGASSLAFIKKSGYDVFIEKSVPLL
                     ERYGDKIVLPLDAAYLDAAGRRVEADVGSIPTDAGVLDIGQKTALLYETVIKNAKTAF
                     VNGPMGVFEKPETELGTKAVWRALGATEAYTVVGGGDSITATEKYGLVEKMGYICTGG
                     GALIRFLTGEELPVAKALRYAAKKFPEGK"
     gene            1628..>2283
                     /locus_tag="PLR57_04600"
     CDS             1628..>2283
                     /locus_tag="PLR57_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HQQ40767.1"
                     /translation="MEIELGFGAGVQKLAVPDENLIGILLPNPVEHALTGEEEVLRAL
                     SDPIGTPRLGEIVHPGEKIAIVTSDITRPMPSYKVLPALLDELYLAGVKKEDITVVFG
                     LGSHRKQTPDEQKKLVGERAFAEVKCIDGDDSDCVHIGTTRRGTPVDIVRPVAEADRR
                     ICLGNIEFHYFAGYSGGAKAIMPGVSTRAAIQSNHSRMVEQAAAAGMLEGNPVREDIE
                     "
BASE COUNT          504 a          624 c          735 g          420 t
ORIGIN      
        1 ccccgcgggg acatgtacga aatcggcctc tggtcggatt ccattgtgga aaccggggac
       61 gaaatcatgt acgccatcaa cattccgcag gagagcgtaa cgattccgga aaccatcgac
      121 ggcatccgcg ccgcctgcgg catgcagcaa acgcgcgaaa cggccaccga tgcaaccaac
      181 gaatatctgg gcatcggccg ctttaaataa gcagtttgct ccgcgcgcga gtcagcgtgc
      241 ggctcagaat tgaatgatgc agcacggcgg gggtgcggaa tccgaacagg gtctccgcta
      301 tcccgccgcg gcgtattccg acttggggga aacagaatat gacacgtttt ggaattcata
      361 ccatggacgc gtacgacgtc cagggcaaga ccgttctctg ccgggtggat atcaaccagc
      421 cggtcgaccg cgtgagcggc aaactcaaga gcgtcaaccg tatccgtgcc tgcgtgccga
      481 ccgtgcggga gctcgcagac cgcggcgcaa aggtcgtttt gctggcgcat cagggaagcg
      541 acatagaata taagaatttt tacaccaccg cgccgcacgc ggaagtgctc tccgaactgc
      601 tggagcgcga agtgaagttt atcgacgacg tgaccggccc cgctgcgcga caggccatcc
      661 gctcgctcaa aaacggtgaa gtgctgctgc tggataacgt ccgattctgc tccgaggagc
      721 agacgctgtt tgaaaccaag ctctgcttaa cgcacgaaca gcaggctaaa acgcaggtcg
      781 tggagaagct cgccccgctg gcggacttgt atgtctgcga cgcgtttgcc gcggcgcacc
      841 gcgaccagcc ttcgctttgc ggattcgagc aggttctgcc cagcatgatg gggcggctgt
      901 tcgagcagga gttctgcgtg atttccggca ttatggaagc gccggaacgc ccgtgcgtat
      961 tcgtgcttgg cggcgcaaaa gtggcggacg catttctgat gatggagact gtgctttccg
     1021 gcggaacggc ggacaaggtg ctcaccggag gcctcgtggc gaacatcctg ctggcggcaa
     1081 aaggcgagca gatcggagca agcagccttg cgtttattaa aaagtccggt tacgatgtgt
     1141 tcatcgaaaa atccgtcccg ttgctggagc ggtacggcga taagatcgtg ctcccgctcg
     1201 acgcggcgta tctggacgcg gcgggcaggc gcgtggaagc ggacgttggg agcattccga
     1261 cggacgcggg cgtgctcgat atcggccaaa agaccgcctt gctgtatgaa acggtcatca
     1321 agaacgcgaa aaccgcgttc gtcaacggcc cgatgggcgt gtttgaaaag ccggaaaccg
     1381 agctcggcac aaaggccgta tggcgcgcgt tgggcgcaac ggaagcctat acggtagttg
     1441 gcggcgggga cagcatcacc gctacggaga agtacgggct tgttgaaaag atgggctata
     1501 tctgcaccgg cggcggcgcg ctgatccgct ttttgaccgg cgaggagctg ccggtcgcca
     1561 aggctctgcg gtatgccgca aagaagttcc ctgaagggaa ataacaaatt agcttaaggg
     1621 ttactatatg gaaatcgaac tcggattcgg cgcgggcgtg caaaagctcg ctgtgccgga
     1681 tgaaaactta atcggcatcc ttctgccaaa tccagtcgag cacgcgctca ccggagaaga
     1741 ggaggttttg cgcgctcttt ccgaccccat cggcacaccg cgcttgggag aaatcgtcca
     1801 ccccggtgaa aagattgcga tcgtcacaag cgatatcacg cgccccatgc cgagctataa
     1861 ggttctgccc gctctgttgg acgagctcta tttagcgggg gtcaaaaagg aagatatcac
     1921 ggtcgtgttc gggcttggca gccaccgcaa acaaacgccg gacgagcaga aaaagctcgt
     1981 cggcgagcgg gcgtttgcgg aggtgaagtg catcgacggg gacgattcgg actgtgtgca
     2041 tatcggtacg acgcgccggg gaacgcctgt ggatatcgtg cgcccggtgg cggaagccga
     2101 ccggcgcatc tgccttggca atattgaatt ccactatttc gcgggttact ctggcggggc
     2161 gaaggccatt atgccgggcg tgagcacgcg ggcggcgatt cagagcaacc acagtcgcat
     2221 ggtggagcaa gccgccgcgg cgggtatgtt ggaaggcaat cctgtgcgcg aagacatcga
     2281 gga
//
LOCUS       DAOMGY010000247         2283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_52815_length_2283_cov_4.046679, whole genome shotgun sequence.
ACCESSION   DAOMGY010000247 DAOMGY010000000
VERSION     DAOMGY010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52815_length_2283_cov_4.046679"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLR57_04605"
     CDS             <1..310
                     /locus_tag="PLR57_04605"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQQ40768.1"
                     /translation="IGNMLLFMPMAVFLPCLFRAMQKFWVFVLAMAGMLVMVEALQLL
                     LSCGSCDVDDILLNLTGTLIVYGILKIPFSKRLLTRLYMLPEPKPVPPPAPEADATAE
                     "
     gene            complement(297..1772)
                     /gene="abc-f"
                     /locus_tag="PLR57_04610"
     CDS             complement(297..1772)
                     /gene="abc-f"
                     /locus_tag="PLR57_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HQQ40769.1"
                     /translation="MSQIRVSDLTFHYEGSYDNIFEHASFSIDTDWKLGFVGRNGRGK
                     TTFLRLLMGELDYDGSISASVTFDYFPFEVEDETLTTSAVFETISGAPRWRLEKELNL
                     LEVEPDGLERAFCTLSPGEQTKVLLAALFSKHGNFLLIDEPTNHLDMEARAAVSRYLN
                     AKKGFILVSHDRAFLDGCVDHILSINRANIEVQKGGFSSWEENRRKQDAFELAENERL
                     TRESEQLKKAARRTAEWADRMESSKIGNGNVEGKPDRGAIGAQSARMMKRAKATQKRM
                     ESAAKQKEQLLKNVERNDTLRVSPLHHHAELLIEARGLTVAYGERTVCGPLDFTLRQG
                     ERLALCGRNGSGKSSIIKLLMGENVPHTGTLRTASGLIVSYMPQDTSFLHGNVLDYAR
                     ENGLDETLFLTILRKLDFPRVQFEKDMRDFSGGQKKKALLAKTLSERAHLYVWDEPLN
                     FVDVLSRMQIRDLLLEAKPSMLFVEHDKSFMDEIATGVIQL"
BASE COUNT          480 a          655 c          626 g          522 t
ORIGIN      
        1 gatcggcaac atgctcctgt ttatgccgat ggcggtgttt ttgccgtgcc tgtttcgggc
       61 gatgcagaag ttttgggtat tcgtgctcgc catggcaggc atgctcgtga tggtggaagc
      121 gctccagctg cttttgagct gcggcagctg cgacgtggac gatattctat tgaacctcac
      181 cggcacgctg atcgtttacg gcatcctgaa gataccgttt tccaagcggc tgctgacgcg
      241 gctctacatg ctgccggagc cgaaaccggt tccgcctccc gcgccggaag ccgacgctac
      301 agctgaataa cacccgtcgc aatctcgtcc ataaacgatt tgtcgtgctc cacaaacagc
      361 atggaaggct tcgcctccaa taataaatcg cgaatctgca tacgggagag cacgtccaca
      421 aagttgagcg gctcgtccca tacatacagg tgcgcgcgtt cggaaagcgt ctttgcaaga
      481 agcgcttttt tcttttgccc gccggagaag tcccgcatgt ccttttcaaa ctggacgcgc
      541 gggaagtcga gcttgcgcag aatcgtcaaa aacagcgtct cgtccagccc gttctcccgc
      601 gcgtagtcga gcacgtttcc gtgcaaaaac gacgtgtcct gcggcatgta ggaaacgatc
      661 agcccgctcg ccgtgcggag cgtgcccgtg tgcggcacgt tttcgcccat gagcagctta
      721 atgatgctgc tctttccgct gccgtttctg ccgcagagcg cgaggcgttc cccctgtctg
      781 agcgtaaagt ccagcggtcc gcagaccgtc cgctcgccgt aggcaacggt taagccgcgc
      841 gcttcgatca aaagttccgc atggtggtgc aggggagaaa cgcgcagggt gtcgttgcgc
      901 tccacgtttt tgagcaattg ctccttttgc ttggccgcgg actccatgcg cttctgcgtg
      961 gctttggcgc gcttcatcat gcgcgcggac tgcgcgccga ttgcgccccg gtcgggttta
     1021 ccctccacgt ttccgtttcc aatcttagaa gattccatgc gatccgccca ttcggctgtt
     1081 cgccgagcgg cttttttgag ctgttcgctc tcgcgcgtga ggcgctcgtt ttccgccagt
     1141 tcaaatgcgt cctgctttct gcggttctcc tcccagctcg aaaaaccgcc cttctgcact
     1201 tcgatattcg cgcggttgat cgagaggatg tgatcgacgc acccgtccag aaacgcccgg
     1261 tcgtgggaaa cgaggatgaa ccccttcttg gcgtttaggt agcggctcac cgccgcgcgg
     1321 gcttccatat ccaagtggtt tgtcggttcg tcgatgagca gaaagttgcc gtgcttacta
     1381 aacagcgcag ccagcagaac cttggtctgt tcgccggggc tgagcgtgca gaacgcgcgc
     1441 tccagcccgt ccggttcgac ctccagcaag ttgagctctt tttccagccg ccatctgggc
     1501 gcgccggaga tggtttcgaa caccgccgag gtggtcagcg tttcatcttc cacctcgaac
     1561 ggaaagtaat caaacgtaac ggaagcggaa atgctgccgt catagtcgag ctcgcccatg
     1621 agcaggcgaa ggaacgtcgt cttgccgcgg ccgttccgcc cgacaaaacc gagcttccag
     1681 tccgtgtcga tggaaaacga cgcgtgttca aaaatattgt cgtaactgcc ttcgtaatga
     1741 aacgttaggt cgctgacgcg aatttgggac atgggaatca tccacctttc tatagtagaa
     1801 gaaaagcgat gccaaaagcg aaacgaaaga tatgatcgat tctgtattga gttcctaaac
     1861 gctaaaaagc aaacaaaaaa gaacccgcat gaaatcggtt cgctcctttg gcatcgcaac
     1921 gacgcgccaa agcgcacttc tgcgcccggc ggggtgatcg aataccgtaa ggagttagcg
     1981 aatcattggt gttcctccgt aaaaaagata cgcttgcagt ttacccaatt aaggcggatt
     2041 tgtcaacatt tgcgccgtgg tattttcatg cgccgcgcgt atgatataat ttcgctgtaa
     2101 cggcaagttt atcatgaatc cacaaaaaaa tttccctccg ccgcaacctc ccgccaggat
     2161 gcgaagtgtt atgggtggaa gagagaggta cgaatccttc atggaacagc tgccaaaggc
     2221 aaccccgccg atcccggcgg acaacgcgga cgcgctcttt gccgagtatg ccgacaaggt
     2281 ata
//
LOCUS       DAOMGY010000248         2278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_52988_length_2278_cov_2.983806, whole genome shotgun sequence.
ACCESSION   DAOMGY010000248 DAOMGY010000000
VERSION     DAOMGY010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52988_length_2278_cov_2.983806"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="PLR57_04615"
     CDS             <1..1427
                     /locus_tag="PLR57_04615"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQQ40770.1"
                     /translation="ANRLLREKEMLDITLQSIGDGVLTTDTAGVIKLINPTAQTLTGW
                     GEDAISRPYSEVLRLVSEETGLSTKSPIETVLQTGETVALGNHTALITRSGGSVPIAD
                     RAAPIRDEEGRIYGVVMVFRDITQEKAQREQIDYIVSHDSMTGLYNRFFMENSILSME
                     REEGLPFAVIMGDLNGLKLVNDAFGHSAGDIFIIKVAEILRECSREGDLVGRWGGDEF
                     LVLMPGGTAADAEQYIDRVLRRCVEESDERIQLSIAMGYAVKTPGDRDFQETLRQAEQ
                     WTYRRKLMIEKSFRNSIINALLSTLSTKSEETEEHAERLQKYCAQIGKILGVSAKELD
                     EMALFAMLHDIGKIGVKDSVLQKPGPLTDEERNEMKKHPEIGFRIAQNNLDLAPVAEY
                     ILCHHERWDGRGYPRGLKGEDIPLLSRILAVVDAFDAMTSERIYRKAVSVNEAAEELM
                     RCAGKQFDPDIVRVFVEQVLGIVA"
     gene            complement(1537..>2278)
                     /locus_tag="PLR57_04620"
     CDS             complement(1537..>2278)
                     /locus_tag="PLR57_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQQ40771.1"
                     /translation="FRLEYALYYMFFLFSMLAGYFDDRSKSSWGEWKKGLIDLAICLG
                     ASLTYLYFNPNPSVSAFGYVLALPAWLFVLIATFLLWVSINFTNCTDGVDGLLGTVSI
                     VSLATFGIVFLLSFHELDMLQMNLIFIAALLAYLMFNASPSRIIMGDAGSRTIGIFLG
                     VMALKTNNLLLYIPAALVIILDGGIGLFKVLVIRITKKKNFLKSIRTPLHDHARYKWG
                     WSDTQVGYRFAIIQLLVSGTCIAAILLK"
BASE COUNT          564 a          585 c          701 g          428 t
ORIGIN      
        1 tcgccaatcg actgctgcgg gaaaaggaaa tgctggatat tacgctccag tcgattggcg
       61 acggcgtgct gacaaccgat accgcgggcg taatcaagct gattaacccg acagcgcaga
      121 cgctcaccgg ctggggagag gacgccatca gcagaccgta ttccgaggta ctgcggcttg
      181 tgagcgaaga aacggggctg agcacgaaat cgcccattga aaccgtgctg caaaccggcg
      241 aaaccgtagc gctggggaat cataccgcgc tgatcacgcg ctccggcggc agcgtaccta
      301 ttgccgaccg cgccgcaccc attcgggacg aagaagggcg catctacggc gttgtaatgg
      361 tatttcgaga cattacgcag gaaaaggcgc agcgcgagca aattgactat atcgtttcac
      421 acgactccat gacggggctt tacaaccgtt tcttcatgga gaacagcatc ctgagcatgg
      481 agcgcgagga aggcttgccg tttgccgtta tcatggggga tctcaacggc ctgaagctgg
      541 tcaacgacgc gttcgggcac agcgcgggag atatctttat catcaaagtt gcggaaatac
      601 tgcgggaatg cagcagggaa ggcgatctgg tcggacgctg gggcggggac gagttcctcg
      661 tgctgatgcc tggcggaaca gcggcggacg ccgagcagta tattgaccgc gtactgcggc
      721 gctgcgtcga ggagagcgac gagcgcatcc agctcagcat cgccatggga tatgccgtga
      781 aaacaccggg ggacagagat tttcaggaga ctttgcggca ggccgagcaa tggacgtacc
      841 ggcgcaaact catgattgag aagagcttcc gaaacagcat tatcaacgct ctgctttcca
      901 cgctttcgac aaagagcgaa gaaaccgagg agcatgccga acggctgcag aagtactgcg
      961 cgcagatcgg taaaattctc ggcgtttccg ccaaggaact cgacgagatg gcgctttttg
     1021 caatgctcca cgatattggc aagattggcg taaaggacag cgttttgcag aagcccggcc
     1081 cgctgacgga cgaagaacgg aacgagatga aaaaacaccc ggagatcggc ttccgcattg
     1141 cgcagaacaa tcttgatctg gcgccggttg cggaatacat cctctgccac catgagcgct
     1201 gggacgggcg cggctacccg cgcgggctga agggcgagga tatcccgctg ctttcgcgga
     1261 tccttgcggt cgtggacgca ttcgacgcaa tgacaagcga gcgcatttac cgcaaagcgg
     1321 tttctgtaaa cgaagctgcg gaggaactga tgcgctgtgc gggcaagcag ttcgacccgg
     1381 atattgtgcg cgtgttcgtt gagcaagtgc tcggaatcgt cgcatagccg ccgcacgaat
     1441 tgaaaaacga aaaaacaggc aggatgttgc gatcctgcct gtttgatgtt tctgttgcta
     1501 tgttcgatga acgaggtttc agcgggcttg catgctttat ttgagcagaa tcgcggcgat
     1561 gcaggttccg ctgaccagaa gctgaatgat tgcgaagcgg tagccgacct gcgtatccga
     1621 ccagccccat ttataccgcg cgtgatcgtg gagcggcgtg cggatggact ttaggaagtt
     1681 cttttttttc gtaatgcgga tgaccagcac cttgaacagg ccgatgccgc cgtcgagaat
     1741 gattacgagc gccgcgggga tatagagcag caggttgttg gttttaagcg ccatcacgcc
     1801 cagaaagatg ccgatggtgc gcgaacccgc gtcccccatg atgatgcggc tgggggaagc
     1861 gttgaacatc aggtacgcca gcagagccgc aatgaagatc aggttcatct ggagcatgtc
     1921 gagttcgtgg aaggagagca gaaagacgat gccgaacgtt gccagcgaaa caattgaaac
     1981 cgtgccgagc agcccgtcca cgccgtccgt gcagttggta aagttaatgg aaacccagag
     2041 caggaacgtg gcgatcagca caaacagcca tgcgggcaac gcgagcacat aaccaaacgc
     2101 ggaaacgctc ggatttgggt tgaaatacag gtaggtcagc gaagcgccaa ggcagatggc
     2161 gaggtcgata agcccctttt tccattcacc ccaggaactt ttgctgcggt cgtcaaaata
     2221 ccccgcgagc atggagaaca ggaagaacat atagtacagc gcgtattcca gacgaaac
//
LOCUS       DAOMGY010000249         2276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_53045_length_2276_cov_3.378658, whole genome shotgun sequence.
ACCESSION   DAOMGY010000249 DAOMGY010000000
VERSION     DAOMGY010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53045_length_2276_cov_3.378658"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PLR57_04625"
     CDS             complement(<1..829)
                     /locus_tag="PLR57_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HQQ40772.1"
                     /translation="MSNPRERLLLTRAYLTEWSDERHIVSPAELIAHLECQGFLADRR
                     TVSADIALLRQSGMRIAEQKSRPFGYYLENRLFEPAEVQLLTDLLRSSRVLTREHSDA
                     LSKKLASLLGRYEAARLRHDWGRKPCAQAVDERTFLNLKRVYEALADGKKLSFHYCQY
                     APEKTLVSRRKGSVYLVNPCMTLFADGNCYLLADHPLHEGFAHYRVDKMADVCVREEA
                     RAPLDPAFDPAQYAKTVFSMYPSEQKWVRLAFDKNLMGAVIDRFGADAAIAELDDTAF
                     "
     gene            complement(860..1861)
                     /locus_tag="PLR57_04630"
     CDS             complement(860..1861)
                     /locus_tag="PLR57_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQQ40773.1"
                     /translation="MAYSAGEKRYERMAYRSCGDSGLKLPLLSLGLWHNFGTHDDYEN
                     MKRMCFSAFDLGVTHFDLANNYGPEPGAAETNFGRILREELAQYRDELVISTKAGYLM
                     WQGPYGDHGSRKYMLASLDQSLKRMGLPYVDIFYHHRMDPETPLEESMLALDTAVKSG
                     RALYAGISNYDAKTALRAAEILKELHCPFVLNQIRYSIFDRHIEPDRLMEAAKQAGFG
                     LITFSPLAQGLLTDRYLGGIPTDSRIGKGGPFLKREQLTEERLAQIKALNDLAAERNQ
                     TLSQMALSWVVRNEAVTSVLIGASSPKQIEENIGILDAPAFTPEQLKRIDTITRYSA"
BASE COUNT          460 a          690 c          627 g          499 t
ORIGIN      
        1 cgaatgcggt gtcgtccagc tccgcaatgg ccgcgtctgc gccgaaccgg tctatgaccg
       61 cgcccatcag gtttttatca aacgcaagcc gaacccattt ttgctcgcta gggtacatgg
      121 aaaacacggt ttttgcgtac tgcgcggggt caaacgcggg atcgagcggc gcgcgggctt
      181 cctcgcgcac gcagacgtcc gccatcttgt ccacacggta atgcgcaaag ccttcgtgca
      241 ggggatgatc ggccagcaga taacaattgc cgtccgcaaa cagcgtcatg cacggattca
      301 ccaggtaaac gctgcctttc cggcgggaaa ccagcgtttt ttccggcgcg tactggcagt
      361 aatgaaacga gagctttttg ccgtccgcca gcgcttcgta aacgcgcttg agattcaaaa
      421 acgtgcgctc gtccaccgcc tgtgcgcagg gttttcgccc ccagtcatgc cgcaggcgag
      481 ccgcctcgta cctgccgagc agcgacgcga gttttttcga aagcgcatcg ctgtgttccc
      541 gcgtcagcac gcggctggat cgcagcagat ctgtcaacag ctgaacttcc gccggttcaa
      601 acagccggtt ttcgagatag tacccgaacg gacgcgattt ctgttcggca atgcgcattc
      661 cgctctgccg cagcagcgca atatccgcgc tcaccgtccg gcggtcggct aaaaagccct
      721 gacattctag atgcgcaatc aactccgcgg gagaaacgat atgccgctcg tccgaccatt
      781 ccgtcaggta tgcgcgggtc aaaagcagtc tctctcgcgg attcgacata accaaactcc
      841 tccggcttac cgcctgtgcc tatgccgaat agcgggtaat cgtatcgatt cgcttgagct
      901 gctcgggcgt gaacgcgggc gcgtcgagga tgccgatgtt ctcctcgatc tgctttggcg
      961 aggaagcacc aatcagcacg ctcgtgaccg cttcgttgcg caccacccag ctcagcgcca
     1021 tctgcgaaag cgtctggttc cgctcggcgg caaggtcgtt gagcgctttg atctgcgcaa
     1081 gcctttcttc cgttagctgt tcgcgcttta aaaacggccc gcccttgccg atccggctgt
     1141 cggttggaat gccgccgagg tagcggtcgg tcagcaagcc ctgcgccagc gggctgaacg
     1201 taatcagtcc gaatcccgcc tgctttgccg cttccatgag ccggtcgggt tcgatatgcc
     1261 ggtcgaagat ggaataccgg atttgattga gcacaaacgg gcaatgcagt tccttgagga
     1321 tttccgccgc acggagcgcc gttttcgcgt cgtaattcga aatgcccgcg tagagcgcgc
     1381 gtccgctctt gaccgccgtg tcgagggcga gcatgctctc ttccagcggc gtctctggat
     1441 ccatgcggtg gtggtaaaag atatccacat acggcagccc catgcgcttc aggctctgat
     1501 ccaggctcgc gagcatatat tttctgctgc cgtggtcgcc gtagggcccc tgccacatca
     1561 gataacccgc cttggtgctg atgaccagct cgtcgcggta ctgcgcgagt tcttcgcgga
     1621 ggatgcgccc gaaattcgtt tccgcggcgc ccggctcggg gccgtagtta ttggcgaggt
     1681 caaagtgtgt tacgcccaga tcgaacgcgg aaaaacacat ccgcttcatg ttttcgtaat
     1741 cgtcgtgcgt gccaaagtta tgccaaagcc ccagcgaaag cagcggcagt ttgagcccgc
     1801 tgtccccgca ggatcgatac gccatgcgct catacctttt ttcgccagcc gaatatgcca
     1861 tactgcgcct cctctttccg cctttgccaa aagtggcagg cggattgtca tgatgcaagt
     1921 attgtaccat acggcgacat tcctggcgcg tcggattcaa aaatcaccgc tttttacagc
     1981 gctgcttggt gccatgctgc ccgtaataac agagcacgca ggaaagcgcc aaaacgttca
     2041 ttaaaatatt gtatttcgtt cattgacttt ccgccgcgaa tgaaagaaaa taggaactgc
     2101 cgagttttgg agccggagaa agcgcgcgta ttccgcgccg tactccccgc ttcgttcggc
     2161 agaatcaaac ggcattcccg gcgcgcggca tacaaccgcc ggccgaagtt ttttattcat
     2221 aggaggacat tcaattggtt accaaagtac ccgtagccac catcgaaatg cagacc
//
LOCUS       DAOMGY010000250         2273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_53162_length_2273_cov_3.417493, whole genome shotgun sequence.
ACCESSION   DAOMGY010000250 DAOMGY010000000
VERSION     DAOMGY010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53162_length_2273_cov_3.417493"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2273
                     /gene="uvrA"
                     /locus_tag="PLR57_04635"
     CDS             <1..>2273
                     /gene="uvrA"
                     /locus_tag="PLR57_04635"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQQ40774.1"
                     /translation="AREHNLKNIDLVVPRDKLVVFTGLSGSGKSSLAFDTIYAEGQRR
                     YVESLSSYARQFLGQMEKPDIDSIDGLSPAISIDQKSTSHNPRSTVGTVTEIHDYLRL
                     LYARCGIPHCPDCGKVIERQSIDQVVDRVLNLPEGTKAQIIAPCVRGRKGEHQKLLDE
                     LKREGYVRVRVDGTVYDLADVPALDKKFKHNIDAVIDRVVIKQGIEGRLTDSLETAFR
                     LGNGLAIVAIVDGEELLFSQNYACPDCGTSIEELAPRMFSFNNPFGACPTCTGLGTLL
                     KIDPDLVIPDRTLSLAQGCVNVMGWNSGSKGTIASMYFEALTAHYGASMKTPFQDLPE
                     EVQNAVLYGTGKDKLHIAYRKEFGSGSFDAPFEGIVTNMERRYRESTSEYSKQEIERY
                     MTHTPCPDCKGRRLKRESLAVTVGETNIADLSELPVKEARTFLSSLRLSKTHMLIAAQ
                     ILKEIDARLGFLVNVGLDYLTLSRSAVSLSGGEAQRIRLATQIGSGLMGVLYILDEPS
                     IGLHQRDNARLLVTLKTMRDLGNTLIVVEHDEETIRSADYVVDIGPGAGEHGGEVVAI
                     GTPEEIAQNEKSITGAYLSGRMSIPVPESRRKGSGKFLTVRGARAHNLKNVDITIPLG
                     MFVCVTGVSGSGKSSLVNEIIKKSLLRDLNRAHTRPGDHDAIEGVDALDKIIDIDQSP
                     IGRTPRSNPATYCGLFDLIREVFANTPDAKMRGYSNGRFSFNVKGGRCEACNGDGIIK
                     IEMHFLPDIYVPCEVCG"
BASE COUNT          492 a          694 c          681 g          406 t
ORIGIN      
        1 gcgcgcgcga acataacctc aagaatatcg acctcgtcgt cccgcgcgac aagctggtcg
       61 tctttaccgg cctgtccggc agcggaaaat cgagcctcgc gttcgatacc atctatgccg
      121 aagggcagcg gcggtacgtc gagagcctat cttcctatgc gcgccagttt ctgggtcaga
      181 tggagaagcc ggatatcgac agcatcgacg ggctttcccc cgcgatctcc atcgaccaga
      241 agagcacgag ccataacccg cgcagcaccg tgggcaccgt aacggagatt cacgactacc
      301 tccgccttct ttacgcgcgc tgcggcattc cgcactgccc ggactgcgga aaggtgatcg
      361 agcgccagtc catcgaccag gtggtcgacc gcgtgctcaa cctgccggaa ggcacgaagg
      421 cgcagattat cgcgccgtgc gttcgcgggc ggaagggcga gcatcagaaa ctgctcgacg
      481 agctcaagcg ggaagggtat gtgcgcgtcc gcgtggacgg aaccgtgtac gatctggcgg
      541 acgttcccgc gctggataaa aagtttaagc acaatatcga cgcggtgatc gaccgcgttg
      601 tgatcaagca gggcattgaa ggccggctca cggattcgct ggaaaccgcg ttccgcctcg
      661 gcaacggcct tgccattgtt gcaattgtgg acggcgagga gctgcttttt tcgcagaact
      721 acgcctgccc ggactgcgga acgagcatag aagagcttgc gccccgcatg ttctccttca
      781 acaacccctt tggcgcgtgc ccgacctgca cgggtctcgg tacgctgctc aaaatcgacc
      841 ccgacctcgt aatccccgac cggacgcttt cgctggcgca gggatgcgtc aacgtgatgg
      901 gttggaacag cggctcgaag ggcacgatag cgagcatgta tttcgaggcg ctgaccgcgc
      961 actacggcgc ttctatgaag acgccgttcc aggacttgcc ggaggaagtg caaaacgccg
     1021 ttctctacgg caccggcaag gacaaactgc atatcgccta ccgcaaggaa ttcggcagcg
     1081 gctcgttcga cgcgccgttt gaaggcatag ttaccaacat ggagcggcgt taccgcgagt
     1141 ccaccagcga gtacagcaag caggaaatag agcggtacat gacgcatacg ccttgcccgg
     1201 actgcaaggg caggcgcctc aagcgcgaaa gcctcgccgt cacggttggg gaaacgaata
     1261 tcgccgacct ttccgaactg ccggtcaagg aagcgcggac gttcctttcg tccctgcggc
     1321 tcagcaaaac ccatatgctc attgcggcgc agattctcaa ggaaatcgac gcgcggctgg
     1381 gctttctggt caacgtcggc ctcgattacc tgacgctttc ccgctcggcg gtgtcgcttt
     1441 ccggcggcga agcgcagcgc atccggctgg ccacgcagat cggcagcggg ctgatgggcg
     1501 ttctgtacat tctcgacgag ccgagcattg gtctgcatca gcgggacaac gcgcgtctgc
     1561 tcgtgaccct gaagacaatg cgcgatctcg ggaatacctt gattgtggtg gaacacgacg
     1621 aagaaacgat cagaagcgca gactatgtgg tggatatcgg gcctggcgca ggtgaacacg
     1681 gcggagaagt ggttgcaatc ggcacgcccg aagaaattgc gcaaaacgaa aaatccatca
     1741 ccggcgcata cctgagcggg cggatgtcca tccccgttcc ggaatcgcgg cgaaaaggga
     1801 gcggaaagtt cctcaccgtg cgcggtgcgc gcgcgcataa cctcaaaaac gtcgatatta
     1861 cgattccgct gggcatgttc gtctgcgtca ccggtgtttc cggcagcggc aaatcgagcc
     1921 tcgtcaacga aatcatcaaa aaatcgctct tgcgcgacct caaccgcgcc catacccggc
     1981 cgggcgatca cgacgccatc gaaggcgtgg acgcgctgga caagattatc gatatcgacc
     2041 agagccccat cggacgcacg ccgcgttcga accccgcgac ctactgcggg ctgttcgacc
     2101 tgattcgcga agtgtttgcc aacacgccgg acgccaagat gcgcggctac agcaacgggc
     2161 gcttcagctt caacgtcaag ggcggccgct gcgaagcctg taacggcgac ggcatcatca
     2221 aaatcgagat gcactttttg ccggacatct acgtcccctg cgaagtctgc ggc
//
LOCUS       DAOMGY010000251         2273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_53167_length_2273_cov_2.825518, whole genome shotgun sequence.
ACCESSION   DAOMGY010000251 DAOMGY010000000
VERSION     DAOMGY010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53167_length_2273_cov_2.825518"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLR57_04640"
     CDS             <1..444
                     /locus_tag="PLR57_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HQQ40775.1"
                     /translation="MKNNYRVEWRRKLPGGGSEDGCGAFNGDLGTIYRLSELDRTISV
                     LFDDERLAVFDFTHLDELDLAYCISIHKSQGSEFPVVILPMFGSASPMMTRNLLYTAV
                     TRAKRQVVCVGRRDALMSMVNNNRTDRRYTALRERLSELFELRKK"
     gene            504..1208
                     /locus_tag="PLR57_04645"
     CDS             504..1208
                     /locus_tag="PLR57_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQQ40776.1"
                     /translation="MSQTQDNERYGLKAFVDGVFDALYPSNVVCRLCNSETILGEDCL
                     CDDCRATLIPCPPMTAPEPLEGLCAAFVYTGGAALGIHALKYAKQTRLAEFFGSAMRL
                     PEDWQIDAVVPVPLHPLKLWIRTYNQSELLAKEVCRRYRLPLEKKLLSRTRFTRTQTA
                     LDAYARTKNVSNAFSASIACAGRSILLVDDVTTTHSTLLACAAALKQAGAAHVYAVCA
                     CSAEKELLRSIKTETE"
     gene            1349..>2273
                     /locus_tag="PLR57_04650"
     CDS             1349..>2273
                     /locus_tag="PLR57_04650"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HQQ40777.1"
                     /translation="MKKIIPSSIRLRKEDFQNLGLNPARVELWEDGMRTDGGRGSYEW
                     WYFDSHLDNGGKLVIVFLTKSFVTPNRPLTPTVTLQYDAPDGTHYEELLTYPAAKFFA
                     SREHCDVKIGENVFSGDLHAYTIRLSGKQVNAELTLTGRVPAWRPGTGQIFFGEHDEH
                     FFAWLPSVPEGSVEGELTINGQSQRITGTGYHDHNWGNVSMLRLMNHWYWGRAKIGEY
                     TVISSFITAEKKYGYRTFPVFLLAKNGIMVAQSTLDSPAFSQSGEHLDDVTKKPVHDE
                     LVYDHTNGNERYVVTYRREKDILRTRFVDTLP"
BASE COUNT          509 a          703 c          609 g          452 t
ORIGIN      
        1 atgaagaaca actaccgcgt ggagtggcgg cgcaagctgc ccggcggcgg ctctgaggac
       61 ggctgcggcg cgtttaacgg cgaccttggc acaatctacc gtctcagcga gctcgaccgc
      121 accatcagcg tcctgtttga cgacgagcgg ctcgccgtgt tcgactttac gcatctcgac
      181 gagctggatt tggcatactg catctccatt cacaagagcc agggcagcga gtttcccgtg
      241 gttattctgc caatgttcgg cagcgcgtcg cccatgatga cgcgaaacct cctgtatacc
      301 gcagtcaccc gcgccaagcg gcaggtcgtc tgcgtcggcc gcagggacgc gctgatgagc
      361 atggtcaaca acaaccgtac cgaccgcaga tacaccgcgc tgcgcgaacg gctcagcgag
      421 ctgttcgaac tgcgcaaaaa atagaatcga agtgcacaaa ttccgaattc cagtaacaga
      481 cattctttaa tggagatcac cagatgagcc aaacgcagga taacgaacgc tatggcctga
      541 aggcctttgt ggacggcgtg tttgacgcgc tgtacccttc caacgtcgtc tgcaggctct
      601 gcaacagcga gacgattctg ggtgaagact gcctctgcga cgactgccgc gcaacgctca
      661 tcccctgccc tcccatgacc gcgccagagc cgctggaggg cctttgtgct gcgtttgttt
      721 ataccggcgg agccgcgctc ggcatccacg cgctgaaata cgctaagcag acgcgccttg
      781 cggaattctt tggcagcgcc atgcgcctcc ccgaagattg gcaaatcgac gcagtcgtgc
      841 ccgtccccct gcatccgctt aaactctgga ttcgaacgta taatcaaagc gaactgctcg
      901 ccaaggaagt ctgccggcgt tatcggctcc cgctggaaaa aaagctcctc tcgcgtacgc
      961 gctttacgcg cacgcagact gcgctggatg cttacgcgcg aacgaaaaat gtttccaacg
     1021 cattctccgc gtccatcgct tgcgccggac gctccatcct gcttgtggac gacgtgacca
     1081 ccacgcacag cacgcttctc gcctgcgccg ccgcgctgaa acaagccgga gccgcacacg
     1141 tttacgccgt ctgtgcctgc tcggcggaaa aagagctgct gcgctcaatt aagactgaaa
     1201 ccgagtgatc tggacatcaa acgtcctgca aacgcggggc gttttgtttt acccgtttcc
     1261 ctcggcttga atttgccaac gcgcttgtgt acaatcaaat cagattttga gtttcgtcga
     1321 tccattcttt tgtggaggtt ggcaaacaat gaagaaaata attccatcca gcatccgcct
     1381 cagaaaagaa gatttccaga acctcggtct gaaccccgcg cgtgtcgagc tctgggagga
     1441 cggcatgcgt accgacggcg ggcgcggcag ttacgaatgg tggtatttcg acagccacct
     1501 cgacaacggc ggcaagctcg taatcgtgtt tctcaccaag agttttgtaa cgcccaaccg
     1561 cccgctgacg ccgaccgtga cgctccagta cgacgcgccg gacggcacgc actacgaaga
     1621 actgctcacc taccccgcgg caaagttctt tgcctcacgc gaacactgcg acgttaaaat
     1681 tggcgaaaac gtcttttccg gcgatctgca cgcctatacg attcgtctca gcggcaagca
     1741 agtaaacgcg gaactcacgc tgaccgggcg cgtacccgcc tggcggcccg gcactgggca
     1801 aatcttcttt ggcgagcacg acgagcactt ctttgcctgg ctgccctccg tgcccgaagg
     1861 aagtgtagag ggcgaactga cgatcaacgg tcaaagccag cgcatcacag gcaccggata
     1921 tcacgaccac aactggggca atgtcagcat gctgcgcctg atgaaccact ggtactgggg
     1981 acgcgctaag atcggcgaat ataccgtgat ttcgtcgttc atcaccgccg aaaagaaata
     2041 cggctatcgt actttccccg ttttcctgct ggccaagaac ggcataatgg ttgcgcagag
     2101 tacgctcgac tcccccgcgt tttcgcaatc gggcgagcat ctcgacgacg tgaccaagaa
     2161 gccggttcac gacgagctgg tatacgatca caccaacgga aacgaacgat acgttgtcac
     2221 ataccgccgc gaaaaggata ttctccgcac gcggtttgtc gatacgcttc ccc
//
LOCUS       DAOMGY010000252         2271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_53232_length_2271_cov_3.726534, whole genome shotgun sequence.
ACCESSION   DAOMGY010000252 DAOMGY010000000
VERSION     DAOMGY010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53232_length_2271_cov_3.726534"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLR57_04655"
     CDS             complement(<1..626)
                     /locus_tag="PLR57_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQQ40778.1"
                     /translation="MKRLLGLLLALMVLLLPAAALAENEYDYDSIPTPNIIVVDGDDP
                     SVVFYERSADQKVYPASTTKIMTCLLALEHGNLDESFMVGDEVYGTSVHFSDYSSKMG
                     IQSGETLTLRDLLYGLMLASGNDAAEAIAKHISGSVDGFVELMNQKAAALGMNGTHFA
                     NPHGVPNDNHYTTARDMAKLMAYALTIDTFVEIAETKTYTVPANNVRST"
     gene            922..1467
                     /locus_tag="PLR57_04660"
     CDS             922..1467
                     /locus_tag="PLR57_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007390382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HQQ40779.1"
                     /translation="MKQFRCPVCGYVHTGETPPDFCPVCKAPGDSFIEIAPEKKPYAA
                     EHVVGVASGVDAEVLEGLRMNFSGECTEVGMYLAMSRVAEREGYPEVAEAFRRYAFEE
                     ADHASRFAELLGEVITTSTKKNLELRAAAEYGACEGKTQLAKRAKELNLDAIHDTVHE
                     MARDEARHGKGFEGLLKRYFS"
     gene            1525..2169
                     /gene="deoC"
                     /locus_tag="PLR57_04665"
     CDS             1525..2169
                     /gene="deoC"
                     /locus_tag="PLR57_04665"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQQ40780.1"
                     /translation="MINDLNTYLDHTLLKADAKPEDILHVVAEAKEFHTASVCVNSCY
                     TALVSAQLKGSGVKSCAVVGFPLGAMSTGAKALEAALTVRDGAEEIDMVMNVGLAKAG
                     DWSAVEKDIAAVVLASKPAIVKVILETCLLTDEEKVKACLAAKSAGAAFVKTSTGFST
                     GGATVEDIALMRKTVGPEMGVKASGGVRTREQAEAMIAAGATRIGCSSTRKIME"
BASE COUNT          532 a          632 c          622 g          485 t
ORIGIN      
        1 gtcgaacgaa cattgttggc cggaacggta tatgtttttg tttcggctat ttcaacaaag
       61 gtatcgatcg tcagcgcata tgccatgagc ttagccatat cccgcgcggt ggtatagtgg
      121 ttgtcgttag gcacgccgtg agggttggca aaatgcgtgc cgttcatgcc aagcgccgcg
      181 gctttctggt tcatcagttc cacgaacccg tcgaccgacc cgctgatatg cttggcaatc
      241 gcctctgccg cgtcgttgcc ggaagccagc atcagcccgt acaaaagatc gcgcagcgtc
      301 agcgtttcac ccgactggat tcccattttc gaggaatagt ccgaaaagtg aaccgaggtg
      361 ccatacacct cgtcgccgac catgaacgat tcgtccaggt tgccgtgctc aagtgcaagc
      421 aggcaggtca taatcttggt cgtgctggcg gggtacacct tctggtctgc gctgcgctcg
      481 taaaacacga cgctcggatc gtcgccgtct actacgatta tgttcggcgt tggaatcgag
      541 tcgtaatcgt attcattttc cgcaagcgca gccgccggaa gcagcagcac catcagagcc
      601 aacagcaagc caaggagtcg tttcattcct atcccccaaa atttcacgga agcgtttttt
      661 gtttggtcat cgttttgcag tatagcagtt caccccctct tttggtagtg gttttttcct
      721 tttgtgattg attgttaaca ttcacgagga tatacttgca gtagagccgc gcggttacgc
      781 atccgcattg tacggcgctt acttttaagc gaaaacacgg cttcccgctc tgttatattc
      841 agtcccgaat aaccatattt ttacgttgca gtcagctttt tgcctcgtcg ggcaagacat
      901 atttgaaagg agattacgct catgaaacag ttccgctgcc ccgtttgcgg gtatgtccac
      961 accggtgaaa ccccgccgga tttttgcccc gtctgcaaag ctcccggaga ttcattcata
     1021 gaaatcgctc ctgaaaagaa accttatgcc gccgaacacg tcgtaggcgt cgcttccggc
     1081 gtggacgccg aagtgctgga aggcctccgc atgaacttct ccggcgaatg cacggaggtt
     1141 ggcatgtacc ttgccatgtc gcgcgttgcc gagcgggaag gctatccgga agtggcggaa
     1201 gcgttccgcc gttacgcctt cgaagaagcg gatcatgcct cgcgttttgc cgagctcctc
     1261 ggcgaagtga tcaccacctc gaccaagaag aaccttgagc tgcgcgccgc cgcagaatac
     1321 ggcgcctgcg aaggcaaaac acagcttgca aagagagcca aggagctcaa cctcgacgcc
     1381 attcacgaca ccgtgcatga gatggcgcgc gatgaagcgc gtcacggcaa aggattcgag
     1441 ggcttgctca agcgctattt ttcctgaaca cacagttgac tggacagcag ttacaatatg
     1501 cagaacatct ggaggaaaca accaatgatc aacgacctga acacctatct tgaccatacc
     1561 ctgctgaagg ccgacgccaa gccggaagat atcctgcacg tcgttgcgga agctaaggag
     1621 ttccacaccg caagcgtctg cgtcaactcc tgctataccg cgctcgtcag cgcgcagctc
     1681 aaaggcagcg gcgtgaagtc ctgcgcagtg gtcggtttcc cgctgggggc gatgtccacc
     1741 ggagccaagg cgctggaagc cgctctcacc gtccgcgacg gagcggagga aatcgatatg
     1801 gtcatgaatg tgggtttggc caaagcgggc gactggagcg ccgtagaaaa ggatatcgcg
     1861 gcggtcgtgc tcgcaagcaa gcccgcaatc gtaaaagtca tccttgaaac gtgcctgctg
     1921 accgacgagg aaaaggtaaa ggcctgcctt gccgcaaaat cggcgggagc agcattcgta
     1981 aagacctcga ccggcttttc tacgggcgga gcgacagttg aagacattgc gcttatgcgc
     2041 aaaaccgtcg gccccgaaat gggcgtaaag gccagcggcg gcgtgcgtac gcgcgagcag
     2101 gcggaggcca tgattgccgc gggagccacg cgcatcggct gcagctcgac cagaaagatt
     2161 atggaataat gaggtaaacc ctctttctac tccatccgaa ctaaaaagtc ccccaaatga
     2221 actgaacccc ggaaaacaga cattgaaaga aaagagcctc ctatcgtagg a
//
LOCUS       DAOMGY010000253         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_53266_length_2270_cov_3.748984, whole genome shotgun sequence.
ACCESSION   DAOMGY010000253 DAOMGY010000000
VERSION     DAOMGY010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53266_length_2270_cov_3.748984"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLR57_04670"
     CDS             <1..711
                     /locus_tag="PLR57_04670"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40781.1"
                     /translation="AIPGDLSYLSDSYPAHADLEEQYYLDYADTDLLEYKTSLIQKIL
                     TVDESTTAMIRIRTQDVVSLIDELFLINDGITQFAGKLDLTKICVMGWSFGGATALDA
                     CVVDNRIQAGINIDGWPYGEQFNAAEAIRQPVLLIRSERRDEMEDIVSGLQLEKLDNP
                     ACVVSIDGAWHENFWDFPYFFPIYHYLGYWGPINPNHLLAIESAYILAFLRASFNDSS
                     ADYAAITAGYQEAELLNP"
     gene            complement(826..1944)
                     /gene="ugpC"
                     /locus_tag="PLR57_04675"
     CDS             complement(826..1944)
                     /gene="ugpC"
                     /locus_tag="PLR57_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HQQ40782.1"
                     /translation="MANVTLKNIKKVYDKNVVAVQEFSLDIADKEFIVLVGPSGCGKS
                     TTLRMIAGLEEITEGELYIGDRLVNDVAPKDRDIAMVFQNYALYPHMTVYENMAFGLK
                     LRKVPKAEIDEKVKEAAEILGITEYLQRKPKALSGGQRQRVAIGRAIVRKPQVFLMDE
                     PLSNLDAKLRNQMRAELIKLRQTVNTTFVYVTHDQTEAMTLGDRIVIMKDGYIQQIGT
                     PQEVFDHPANLFVAGFIGSPQMNFFDAELVVSSGKYAVKLEDATIDLPQDIQRTLKAK
                     NVAGGKIVLGIRPEHVKLTNGRSKDSIAAKVDVSEMMGSELYVHVTAVGKDSVMRIAT
                     VDLPAENRYGFKYGDQLNFTFDGSLAHLFDPETGLNLL"
     gene            complement(2077..>2270)
                     /locus_tag="PLR57_04680"
     CDS             complement(2077..>2270)
                     /locus_tag="PLR57_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40783.1"
                     /translation="ADGKIIIPLFMRLVCSAAKSAQNRVVITPHGLPSGRKSAIIGTA
                     YLVSKRTAFTPLLSRRDAE"
BASE COUNT          488 a          601 c          612 g          569 t
ORIGIN      
        1 gcgattcccg gtgacctttc gtatctgtcc gattcatatc cggcgcatgc tgatcttgag
       61 gaacaatact atctggatta tgcagatacc gacttgctgg agtataaaac atcgttgatt
      121 caaaagattt taaccgtcga tgaatcgaca actgcgatga ttcgcatacg aacacaggac
      181 gtagtatctc tgatagacga actgtttctt ataaacgacg gcataacaca atttgccgga
      241 aagctggatc tgaccaaaat ctgtgtcatg ggctggtcat ttggcggtgc aacagcattg
      301 gatgcgtgcg ttgttgacaa ccgtattcag gctggtatca atatcgacgg gtggccgtat
      361 ggcgagcaat tcaacgccgc cgaagcaatt cgccagcccg ttctgttgat acgcagcgag
      421 cgccgggacg agatggaaga catcgtgagt ggtttgcagc ttgaaaaact ggataacccg
      481 gcctgcgtcg tcagtattga cggtgcttgg cacgaaaatt tctgggattt cccttacttc
      541 tttccgatct accactatct cggatattgg ggacccatca atcccaatca ccttctggcg
      601 attgagtccg catatattct cgcattcctg cgtgcatcgt tcaacgacag ttcagctgat
      661 tatgcagcga tcacggcagg gtatcaggag gcagaattgc tcaacccata accgttctgt
      721 acacccgtgt aaaaaccttc ctttgggatg gttctttcaa atatcaagcc attctctcat
      781 gaaaaaaagc tcccgcgaca tgcgggagct tcataacaaa ttgtgctaga gcagatttag
      841 gcccgtttcc ggatcgaaca ggtgcgcaag cgaaccatcg aacgtgaagt tgagctgatc
      901 gccgtacttg aacccgtagc ggttctcggc gggcaggtct acggttgcaa tacgcatgac
      961 gctgtcctta ccaaccgccg taacgtggac atacagctcg ctgcccatca tttcggagac
     1021 gtccaccttc gcggcaatgg aatccttgct tctgccgttg gtgagcttga catgctcggg
     1081 gcggatgccc agaacgatct taccgccggc gacattcttt gctttcagcg tgcgctggat
     1141 atcctgcggc aggtcgatgg tggcatcctc gagcttgacg gcgtacttgc cgctcgaaac
     1201 gacgagttcc gcatcgaaga agttcatctg cggcgaaccg ataaatcctg caacgaaaag
     1261 gtttgccgga tggtcgaaca cttcctgcgg ggtgccgatc tgctggatgt agccatcctt
     1321 catgatgaca atccggtcgc ccagcgtcat agcttccgtc tggtcgtggg taacgtacac
     1381 aaacgtagtg ttgacggtct ggcgcagctt gatcagttcg gcacgcatct ggttgcgcag
     1441 cttagcgtcg aggttactga gcggttcgtc catgaggaag acctggggtt tacggacgat
     1501 ggcgcggccg atggcaacgc gctgtctctg gccgccggaa agcgctttgg gctttctctg
     1561 gaggtactcg gtgatgccca ggatctccgc ggcttccttg accttttcgt cgatctcggc
     1621 cttgggaacc ttgcgcagct tgagcccgaa cgccatgttt tcatacacgg tcatgtgcgg
     1681 gtagagcgcg tagttctgga agaccatcgc gatgtcgcgg tctttgggcg cgacgtcgtt
     1741 gacgagtcgg tcgccgatgt agagttcacc ttcggtgatc tcttcgagcc cggcgatcat
     1801 gcgcagagtc gtcgacttgc cgcagcccga gggcccgact agcacgatga actctttgtc
     1861 cgcgatgtca agagaaaact cctgcaccgc tacgacattc ttgtcgtaga ccttcttgat
     1921 gttcttgagt gtaacgtttg ccatgtgttt ttcggctttg gcgggcgcaa cattcgttca
     1981 tcgcccgact cgcgtaccgc ccgtaaaagt ggcggacgca ttacggcagg tctccacact
     2041 gccgcaccgc aagccatgcg gttggttatc cctcctttat tcagcgtcgc ggcggctaag
     2101 aagtggagta aacgccgtgc gcttggatac aagataggct gtacctatta ttgcagattt
     2161 tctcccgctt ggcaagccgt gcggcgtaat aaccacgcga ttttgtgcgc tttttgctgc
     2221 cgaacatact aatcgcatga acaaaggaat aattatcttc ccgtcagcag
//
LOCUS       DAOMGY010000254         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_53283_length_2270_cov_3.025734, whole genome shotgun sequence.
ACCESSION   DAOMGY010000254 DAOMGY010000000
VERSION     DAOMGY010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53283_length_2270_cov_3.025734"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PLR57_04685"
     CDS             <1..1009
                     /locus_tag="PLR57_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40784.1"
                     /translation="DGYAMKPVAGKDGWYTLTVALTPEVRDTTYDGHYYKVTNGTWDA
                     TGCWGADNYALQPAPVSPTGGGLGSIWVYNDGTLTVLFDSVNMVIYDDSMTQAFATPR
                     IYGDFNTAMARGTDWSLADGEALELTDGNGDGVFEGLYKLPAYTGTGEGYSMVLAVSQ
                     KYYIYAGNGAWGAAEQYLFDGTAAGMGTVSYLKPDADTIYEFKYDRATHITTATAVSK
                     DDVQALAGPTAYGDFSLWLYEGGSSVLLKDDDGDGVFTGTLTLPAYTGADQGYMIAIA
                     LSKKLYDDDYGIRWGTEEQYKFDGTAAGMGGLSYLNPTADTTYTLSYDSATHITTVTE
                     G"
     gene            1082..2110
                     /locus_tag="PLR57_04690"
     CDS             1082..2110
                     /locus_tag="PLR57_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HQQ40785.1"
                     /translation="MFRKQDRQPNFENLRLVLERKAPPRPVLFDFIIGADKEKLLTGE
                     DYRTETEFERVVTTIRAFDSAGYDHAPIIVRGLDFKKNAAHQEKTKSLNDGAAVASRE
                     DFNRFVWPEITDCDFSIIQRAGEFVPKGMKLIPFAYDGILENTISILGYESLCLMLYD
                     DPRLVEDVFFEVGSRVKQYYEQCLAFDEVGAILCNDDWGFGAQTMVSPDTLRKCVFPW
                     YREIVKAAHERGKPALLHSCGAYQAIIGDITDIMHFDGRHSYEDKIVPVETAYAQLHE
                     HIAVLGGIDVDFLARKTPEEVYARCRNLIALSAKEGGYALGSGNSVPDYIPNENFIAM
                     LRAANEVY"
BASE COUNT          508 a          680 c          660 g          422 t
ORIGIN      
        1 ggacggctac gctatgaagc cggtcgcggg caaggacggc tggtacacgc tgaccgtcgc
       61 tctcacgccg gaagtgcgcg atacgactta tgacgggcac tactacaaag tcaccaacgg
      121 cacctgggac gcgaccggat gctggggcgc ggacaactac gcgctgcagc ccgcgcccgt
      181 ctcccccacg ggcggcggcc tcggctcgat ctgggtctat aacgacggca cgctgacggt
      241 gctctttgac tccgtcaaca tggtcatcta cgacgattcc atgacgcagg cgttcgcaac
      301 cccgcgcatc tacggcgact tcaacaccgc catggcgcgc ggcaccgact ggagccttgc
      361 cgacggcgaa gcgctcgaac tcacggacgg caacggtgac ggcgtgttcg aaggtctcta
      421 caagctgccc gcttacaccg gcacgggcga aggctactcc atggttctcg cggtttcgca
      481 gaagtactat atctatgcgg gcaacggcgc ttggggcgcg gcggaacagt accttttcga
      541 cggcacggcc gccggaatgg gcaccgtgag ctacctcaag cccgacgcgg acaccatcta
      601 cgagttcaaa tatgacagag caacgcacat caccaccgcg accgccgtga gtaaggacga
      661 tgtgcaggct ctggccggcc ccacggcgta cggcgatttc tcgctctggc tctacgaggg
      721 cggctcctcc gtcctgctta aggatgacga cggcgacggc gtgtttaccg gcacgctgac
      781 tctgcccgcc tacaccggcg cggatcaggg ctatatgatc gccatcgcgc tctccaaaaa
      841 gctctatgac gacgattacg gcatccgctg gggcacggaa gaacagtata agttcgacgg
      901 cacggcggcg ggcatgggcg gcttaagcta cctcaacccg acggcggaca cgacctacac
      961 cctgagctac gacagcgcaa cccatatcac aaccgtaacg gaaggctaaa atcgtccttc
     1021 ctgaccaaaa tcacaagccc ccgctcgtca gtcgggcggg ggcggctttt taggagcaga
     1081 catgtttaga aaacaggatc gtcagccgaa ttttgagaat ctccgcctgg ttctggagcg
     1141 taaagctccg ccgcgccccg tactgtttga tttcatcatc ggagcggata aggagaaact
     1201 gcttacaggc gaagactacc gcacggaaac cgaattcgag cgcgtcgtca ccaccatccg
     1261 cgcgttcgac agcgcggggt acgaccatgc gccgatcatc gtgcgcggtc tggactttaa
     1321 aaagaacgcg gcgcatcagg agaaaacgaa gtcgctcaac gacggcgcgg cggtcgcctc
     1381 ccgcgaggac tttaaccgct ttgtctggcc ggaaatcacg gactgcgact tttcgatcat
     1441 tcagcgggcg ggagaattcg taccaaaggg catgaagctg attccgtttg cttacgacgg
     1501 tatcctcgaa aacacgatca gcatcctcgg ctatgagtcg ctctgtttga tgctctacga
     1561 cgatccccgg cttgtcgagg acgtattctt tgaagttggc agccgcgtga aacagtatta
     1621 cgaacagtgc ctcgcgttcg acgaagtcgg cgcaattctc tgcaacgacg actggggctt
     1681 tggcgcgcag accatggttt cgccggatac actgcgcaag tgcgtctttc cctggtaccg
     1741 ggagatcgtc aaagcggcgc acgaacgcgg aaagcccgcg cttttgcatt cctgcggcgc
     1801 gtaccaagcg atcatcggcg acattactga catcatgcac tttgacgggc ggcattccta
     1861 tgaggacaag atcgtccccg ttgaaacagc atacgcacag ctgcatgagc atatcgcggt
     1921 actgggcgga atagacgtgg actttctcgc gcggaaaacg ccggaggaag tctatgcccg
     1981 ctgccgtaac ctgatcgcgc tttccgcaaa agagggcggc tacgcccttg gcagcggcaa
     2041 cagcgtgccg gactatattc cgaatgagaa ctttatcgcc atgctgcgcg cggcaaacga
     2101 agtgtattga tcaagatata tgttcttgtg ctcctgccgt aaaagcgcat aagcgcagca
     2161 cggcgcgttc ttctcaaacg cgtcggcaac ccggtcgagc gagcacagcg ctggccgagg
     2221 aaaaagttat aattatggaa aagaaagggc tcaatcacaa aacttgtggg
//
LOCUS       DAOMGY010000255         2268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_53343_length_2268_cov_3.206959, whole genome shotgun sequence.
ACCESSION   DAOMGY010000255 DAOMGY010000000
VERSION     DAOMGY010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53343_length_2268_cov_3.206959"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PLR57_04695"
     CDS             complement(<1..330)
                     /locus_tag="PLR57_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40786.1"
                     /translation="MPSLSAQILKLQLRLAKPLARFTTIEAARIAQDQLGRVTAGVLK
                     TKVTYEDVSFDNFQACFAVSHECIAPCDRVILYLHGGGYTAGQLDYAKGFGGLLAAAT
                     RISVFCVA"
     gene            complement(480..1076)
                     /locus_tag="PLR57_04700"
     CDS             complement(480..1076)
                     /locus_tag="PLR57_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HQQ40787.1"
                     /translation="METGMRNPSVDDFLLGEKKWPAELLYLRGIALESGLSETLKWGK
                     PCYMLEGANVAILYSFKDTCAIGFLKGALLRDEQKLLVAPGEHSQAMRMLKYTSVQQI
                     AAQEQFIRAYLREAVELERSGAEIVFTEHETMEPPEELKEKFRTMPELEAAFLQLTPG
                     RQRAYLMYFSQAKQSETRVSRIEKYIPVILSGKGLNDR"
     gene            complement(1329..1673)
                     /locus_tag="PLR57_04705"
     CDS             complement(1329..1673)
                     /locus_tag="PLR57_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HQQ40788.1"
                     /translation="MDDCIFCKIASGAIPSKKIYEDDQLIAFHDIAPQAPVHVLVVPK
                     KHIPGVNELAADDLALVGHVYLVIQQLVRELKIDESGYRVVVNSGKDGSQSVPHLHFH
                     VLGGRSLAWPPG"
     gene            complement(1741..>2268)
                     /locus_tag="PLR57_04710"
     CDS             complement(1741..>2268)
                     /locus_tag="PLR57_04710"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ40789.1"
                     /translation="KICRQFHLSLQSGSDTVLKRMNRRYTSAEYLHAVEFLRDEMLDC
                     AVTTDIIAGFCGETEAEHRKTLAFCERAAFARMHVFPFSVRSGTKAATMPGQLPKAVK
                     DARAKELIALGKRLTEQYLASQTGSVVEVLVESDGGGYSGNYIRVQTDAPEGEIVRIR
                     LDGFKGETAFGTRVS"
BASE COUNT          470 a          655 c          611 g          532 t
ORIGIN      
        1 agccacgcag aaaacgctga tgcgcgttgc cgccgcgagc agtccgccaa agcccttggc
       61 gtagtcaagc tggcccgccg tgtacccgcc gccgtggaga tacaaaatca cccggtcgca
      121 gggggcgata cattcatgag acacggcaaa gcaggcctga aagttatcaa acgaaacgtc
      181 ttcgtaggta accttggttt ttaacacccc cgccgtcacc ctgcccaact gatcctgcgc
      241 gatgcgcgcc gcttcgatgg tggtgaaccg cgcgagcggt ttggccagcc ggagctggag
      301 tttgagtatc tgcgcgctga gacttggcaa tgtgtcaagt tcctttcgtg aatcgatact
      361 ctattctagc atactttgcg acatttcctt ctttttacag gcggcttctt tttttagcag
      421 gaacttctgc aaaagttgct ctttttgctt ctttttcttt tagaaaaaga agagtatccc
      481 tacctgtcgt tcagcccctt gccgctcagg ataacgggaa tgtacttctc gatgcgggaa
      541 acgcgcgttt cgctctgctt cgcctgcgaa aaatacatca ggtacgcgcg ctggcgcccg
      601 ggcgtgagct gcagaaacgc ggcttcgagc tcgggcatcg tgcggaattt ctccttcagc
      661 tcttcgggcg gctccatggt ctcgtgctcg gtaaacacga tctccgcgcc ggaacgctcc
      721 agttcgacgg cctcgcgaag gtacgcgcgt atgaactgct cctgcgccgc aatctgctgt
      781 acgctggtat acttgagcat ccgcatggcc tgcgagtgct cgccgggggc gacaagcagc
      841 ttttgctcat cgcggagcag cgcacctttc aaaaagccga tggcgcaggt gtccttaaag
      901 gaataaagga tggcgacgtt cgcgccttcc agcatgtagc agggcttgcc ccacttgagc
      961 gtttcgctca ggccgctttc cagcgcgata ccgcgaagat acagcagttc ggcaggccat
     1021 ttcttttcgc caagaagaaa gtcgtccacg ctcgggtttc tcattccggt ttccatactc
     1081 gacgcctcgc ttttttcaaa atgtattatg aatcgtgttc gtcaaatctg tttcaacaat
     1141 cccgccaatc tttgtgaaac aatgtgaagt ttctgcttga ccaattggac gcacctagga
     1201 aaaacaagcc acggtaaatg atatgttcta tatacgcatt atacagaaaa gaacaaggaa
     1261 gagccatata tgccgctctc ccttgcattg tgtttctgaa attgtacgaa gacgcattga
     1321 ttcccacctc agcccggagg ccatgcaagc gagcgcccgc cgagtacgtg aaaatgcaga
     1381 tgcggaacgc tctgcgaacc gtccttgccg ctgttgacca cgacgcggta gccggattcg
     1441 tcaatcttga gctcgcgcac aagctgctgg atcacgaggt agacgtgccc gaccagagcc
     1501 aaatcgtcgg cggcgagttc gttgacaccg gggatgtgct ttttcggcac gacgagaacg
     1561 tgcaccggcg cctgcggcgc aatgtcgtgg aaggcgatca gctgatcgtc ctcgtagatc
     1621 tttttggacg gaatcgcgcc ggacgcgatt ttgcagaaga tgcagtcgtc catgtttgtt
     1681 tcaccccttt cgtgtaatgc aaatcaacac tcctgaatca ggaatatttt gatggttatc
     1741 ttatgaaacc cgcgtgccga acgccgtttc gcccttgaac ccgtcgaggc ggatgcggac
     1801 gatttcaccc tccggcgcgt cggtttgcac gcggatgtag tttccgctgt agccgccgcc
     1861 gtcgctttcc accagcactt ccacaacgct gccggtttgg gaagcgaggt attgctccgt
     1921 gaggcgcttg ccaagcgcga tgagctcttt tgcgcgcgcg tccttaaccg ccttggggag
     1981 ctgccccggc atggtggccg ctttggtgcc actccgcacg gaaaagggga acacatgcat
     2041 ccgcgcaaac gccgcccgtt cgcagaacgc gagggttttg cggtgctccg cttccgtttc
     2101 gccgcaaaag cccgcgataa tgtccgttgt gacggcacag tccagcatct cgtctcgtaa
     2161 aaactccacc gcgtggaggt attccgcgct cgtatagcgc cggttcatac gcttgagcac
     2221 cgtgtccgac ccgctctgca aagaaagatg aaactgcctg cagatctt
//
LOCUS       DAOMGY010000256         2255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_53754_length_2255_cov_3.459545, whole genome shotgun sequence.
ACCESSION   DAOMGY010000256 DAOMGY010000000
VERSION     DAOMGY010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53754_length_2255_cov_3.459545"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1075
                     /locus_tag="PLR57_04715"
     CDS             170..1075
                     /locus_tag="PLR57_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HQQ40790.1"
                     /translation="MKNDRLFQMVYILLEKGSVTAPELASTLEVSVRTVYRDVEALSM
                     AGVPVYASQGKGGGISLMPNYSFDKALLSDDEQNQILFAIQSLQATDQPVGALLKKLG
                     GMFQKQNVSWIEADFSRWGMGRVDNEKFERLKTAILEKRVLEILYCSTSGETAKRSIL
                     PFKLIFKDKSWYLQAFCKKADDYRLFKVNRIVELEVTSERFTESFDDAPPMELDMLPV
                     GAFTTIRLRFSPAVAFRVYDEFDRRQVSPQADGSMVVETSLPADDWVAHYLFSFGTEV
                     EILSPPELKQELEAYAKKIYEHYKT"
     gene            1144..1410
                     /locus_tag="PLR57_04720"
     CDS             1144..1410
                     /locus_tag="PLR57_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018757441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQQ40791.1"
                     /translation="MEERYCQSCGMPMGPTDEHYGTEADGSKSKDYCKYCYQNGAFTA
                     DCTMQEMIDFCVQPMVENMPGMTEAQARAIMNEHFPQLKRWAAK"
     gene            1465..1896
                     /locus_tag="PLR57_04725"
     CDS             1465..1896
                     /locus_tag="PLR57_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HQQ40792.1"
                     /translation="MKAPEQIEESMLAPCGLNCTLCYRHVGKNPCPGCRARVSEPEKY
                     IRKCVMRACTAEQGLTLCAECGRFPCARVKSFDKRYTSGYGVSPMDDGAFAAANGINA
                     LFERQLKTYTCSVCGGLIDLHSGVCSECKKQYPLGKGRNHP"
     gene            1893..>2255
                     /locus_tag="PLR57_04730"
     CDS             1893..>2255
                     /locus_tag="PLR57_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HQQ40793.1"
                     /translation="MTWQQRYPKAIEPSLQEIDGFIDSPLWKELRAYIEGAYCVEPRV
                     EYSTCSGAPGWNVKYKKSGRSLCTLYPDEGAFTALVTIGTKEAPEAELLLPLCSEYLR
                     ELYTRAGALNGARWLMIRV"
BASE COUNT          550 a          643 c          635 g          427 t
ORIGIN      
        1 taaacgtttt gtatcccacc acattttact tcataaacac actctaaaga agtaaaattc
       61 ttttgcttct tttcttttaa gaaaagaaga gcactcccca aaaacctgac atccccatgt
      121 ccggtttttc tgctatactc agcctataaa gatcaaagaa ggtttccgct tgaaaaacga
      181 ccgtctgttt caaatggtat acatcctgct cgaaaaaggc tccgtcaccg cgccggagct
      241 ggcaagcacg ctggaagtct ccgtccgcac ggtgtaccgg gacgtagagg cgctgtccat
      301 ggcgggcgtt ccggtgtatg ccagccaggg caaaggcggc ggcatttcgc tgatgccgaa
      361 ctactcgttc gacaaggctc tgctttcgga cgacgagcag aaccaaatcc tgttcgccat
      421 ccaaagcctg caggcgacgg accagccggt gggcgcgctg ctcaaaaagc tcggcggcat
      481 gtttcaaaag caaaacgtca gctggatcga agcggacttt tcgcgctggg gcatggggcg
      541 cgtggacaac gagaaattcg agcgcctcaa aaccgccatt ctggaaaagc gcgtgctgga
      601 gattctctac tgcagcacgt cgggcgaaac ggcaaagcgc agcatcctgc cgttcaagct
      661 gatctttaag gacaaaagct ggtatctgca ggcgttctgc aaaaaggccg acgactaccg
      721 cctgttcaag gtcaaccgca tcgttgagct ggaggtcaca agcgagcgct ttacggaatc
      781 gtttgacgac gcgccgccaa tggagctgga tatgcttccg gtcggcgcgt ttacaaccat
      841 ccggctcagg ttctcgcccg cggttgcctt ccgcgtgtac gacgagttcg accgcagaca
      901 ggtgagcccg caggcggacg gctccatggt cgttgaaacc tcgctgcccg cggacgactg
      961 ggtggcgcac tacctgtttt cgttcggcac ggaagtggaa atcctctcgc cgccggaact
     1021 caaacaagag cttgaagcgt acgcaaaaaa aatctacgag cactacaaaa cctgacgtac
     1081 cgtgtcaggt ttccgccggt atactggagc catcctacaa caattcagga ggcgagaaca
     1141 acaatggaag aacggtattg ccaaagctgc ggaatgccca tggggccgac ggacgaacac
     1201 tacggaacgg aagcagacgg ctcaaagagc aaggactact gcaaatactg ctatcaaaac
     1261 ggcgcgttca ccgcggattg caccatgcag gaaatgatcg atttttgcgt ccagccgatg
     1321 gtggaaaaca tgcccggcat gacggaagcg caggcgcgcg ccatcatgaa cgagcacttc
     1381 ccgcagctca aacgctgggc ggcaaagtaa gaaggatttc gcttcaccaa aattcgactg
     1441 ccgcgcacgg cggagggagc caacatgaaa gcgccggaac aaatcgaaga atccatgctc
     1501 gcgccctgcg ggctcaactg caccctttgc taccgtcatg tgggcaaaaa cccctgcccc
     1561 ggctgccgcg cgcgggtttc cgaaccggaa aagtacatcc gaaagtgcgt catgcgcgcc
     1621 tgcaccgccg aacaggggct gaccctctgt gcggaatgcg ggcgctttcc ctgcgctagg
     1681 gtaaaatcgt ttgataagcg ctatacatcc ggctacggcg tcagtccaat ggacgacggc
     1741 gcgtttgcgg cggcaaacgg tataaacgcg ctctttgaac gccagttgaa aacgtatacc
     1801 tgctccgttt gcgggggtct gatcgacctg cacagcggcg tctgcagcga atgcaaaaag
     1861 caatatccat tgggaaaggg gcgaaatcat ccatgacctg gcaacagcgt tacccgaaag
     1921 cgattgagcc aagcttacag gagatcgacg gctttatcga ttccccgctc tggaaggaac
     1981 tgcgcgctta cattgaaggg gcttactgcg tggagccgcg cgtagagtac agcacctgtt
     2041 ccggcgcgcc gggctggaac gtcaagtaca aaaaaagcgg acgttcgctc tgcacgctct
     2101 accccgacga aggcgcgttt accgcgctgg ttaccattgg aaccaaggaa gcgccggaag
     2161 cggagctctt gctgccgctc tgctcggagt acctgcgcga actctacacg agagcgggcg
     2221 cgctcaacgg cgcgcgctgg ctgatgatcc gcgtg
//
LOCUS       DAOMGY010000257         2252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_53850_length_2252_cov_3.544379, whole genome shotgun sequence.
ACCESSION   DAOMGY010000257 DAOMGY010000000
VERSION     DAOMGY010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53850_length_2252_cov_3.544379"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1912
                     /locus_tag="PLR57_04735"
     CDS             <1..1912
                     /locus_tag="PLR57_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40794.1"
                     /translation="AEDAKYVIEYLRARDYGRATLLPMALLNPTRPTREERAMLDLPG
                     CIGFASELVGCDDNVRDVIDYLLCRTIIVKDLDAGIALKKRTRGAFHIATLEGDIIST
                     GGSMSGGSLQKQSHSLLGREREIKELRETLKKAEAALEEKKRSLRKNEQELLQCDIQV
                     DSFRDAVHACEIEAAKQAEQLDIIRRDVEKSRESEAELEAERLQLSENLTDVERERKS
                     ADESQTTIEQDNASTREDVVKAQGELAELRKERERAANELSEQKVRRMALSKERDAVY
                     AEQKRLVNEHRELSIKLQKLEKEEAENEAAIGQIEAELKGMLAEIDSDQKVNGAEKEA
                     QKKLEEERLSVSNSLSELRKRREELLTGARDLSERIHKQEMQQAKLEMELAAMQDHIW
                     EEYELTYENAASLRREIAIGATNTRIGEIKAEVRELGDINLGSIEEYKIVAERHTSLK
                     TQFDDLQKAKADLQQLILELTATMETVFLKQFELIQKNFSSVFAELFGGGFAELRLTD
                     KEDVLGCDIDIIAQPPGKKLQLLTLLSGGERALTAIALLFAMLMLKPPAFCMLDEIES
                     ALDEANVSRFADYVKRYSDGTQFILITHRKGSMEVCDTLYGVSMEEKGVSKVVSARFG
                     ESGDSANVMQAD"
     gene            2035..>2252
                     /locus_tag="PLR57_04740"
     CDS             2035..>2252
                     /locus_tag="PLR57_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HQQ40795.1"
                     /translation="MEKKPLFQKWKTGLGKTRTSLLARLFGAKDGVNEAFYEELEEAL
                     ILADVGAEATERLLDGLQRALGSHPDVVA"
BASE COUNT          580 a          614 c          691 g          367 t
ORIGIN      
        1 cgccgaggac gcaaaatacg ttatcgaata cctccgcgcg cgcgactacg gcagagcgac
       61 gcttttaccc atggcgcttt taaatcccac ccgcccgacg cgggaggagc gcgccatgct
      121 cgatctgccc ggctgtatcg ggtttgccag tgagttggtc ggctgcgacg acaacgtgcg
      181 ggacgtgatc gactacctgc tctgccggac gatcattgtt aaggacttgg acgcgggtat
      241 tgcgctcaaa aaacgcacgc gcggcgcgtt ccatattgcc accttagaag gcgatatcat
      301 cagcaccggc ggctccatga gcggcggtag cctgcaaaag cagagccatt cgctgttggg
      361 cagagagcgc gaaattaagg aactgcgcga aacgctcaag aaagcggaag ccgcgctgga
      421 agaaaagaag cgttccctgc gcaagaacga gcaggaactg ctccagtgcg atatccaggt
      481 ggattcgttt cgggacgcgg tgcacgcctg cgaaatcgaa gcggccaagc aggccgagca
      541 gcttgatatc atccgtaggg acgtggaaaa gagcagggag tccgaagccg aactggaagc
      601 ggaacgatta cagctcagcg aaaacctcac ggacgtggag cgcgagcgca agagcgcgga
      661 cgaaagccag accaccatcg agcaggacaa cgcctccacc cgcgaggacg tggtaaaagc
      721 gcagggcgaa ctcgccgaac tgcgtaagga gcgcgaacgc gccgccaacg agctctccga
      781 gcagaaggtg cgtagaatgg cgctctccaa ggagcgcgac gcggtctacg ccgagcagaa
      841 gcggcttgtc aacgagcacc gcgagctttc gattaagctc cagaagctcg aaaaggaaga
      901 agcggaaaac gaagccgcca tcggccagat cgaggcggaa ctcaagggaa tgctcgccga
      961 aatcgactcc gaccaaaagg tcaatggcgc ggaaaaggaa gcgcagaaga agctggaaga
     1021 ggagcgtctg agcgtttcca attcactcag cgaactgcgc aaacgccgcg aggaactctt
     1081 aaccggcgcg cgcgaccttt ccgagcgcat ccataagcag gaaatgcagc aggccaagct
     1141 cgaaatggag cttgccgcca tgcaggatca tatttgggaa gaatacgaac tgacctacga
     1201 aaacgcggcg tccctgcggc gggaaatcgc catcggggcg accaacacgc gcattggcga
     1261 gatcaaggcc gaagtgcgcg agctgggcga catcaacctt ggctcgattg aggaatacaa
     1321 gatcgttgcc gagcggcata cctcacttaa aacgcagttc gacgatttgc agaaagcaaa
     1381 agccgacctc cagcagttga ttctggaact caccgcgaca atggagaccg tgttcctcaa
     1441 gcagtttgag ctgattcaga agaacttttc aagcgtgttt gcggagctct ttggcggcgg
     1501 gtttgccgaa ctaaggctga cggacaagga agacgtgctc ggctgcgata tcgacattat
     1561 agcccagccg ccgggaaaga agctgcagct attgaccctg ctctccggcg gggaacgcgc
     1621 gctgaccgcc attgcgctgc tgtttgcaat gctcatgcta aagccgcccg cgttctgcat
     1681 gctcgacgaa atcgagtccg cgctggacga ggcgaacgtc agccgctttg cggactacgt
     1741 caagcgctac agcgacggca cgcagttcat cctgattacg caccgcaagg gcagcatgga
     1801 agtctgtgac acgctctatg gcgtttccat ggaggaaaag ggagtgagca aggtcgtttc
     1861 cgcgcgcttc ggcgaaagcg gagactcggc gaacgtcatg caggcggact gagaatttct
     1921 aaaaatgctt ttttcaagag catttttaga aatgagattg taaacgtaaa tagttatcgc
     1981 cggaagggcg gtcagggaga aaatattcgc ccgaagggcg aacaggtaaa tagaatggaa
     2041 aagaaacccc tctttcaaaa atggaaaacc ggcctcggca aaacgcggac gagcctgctc
     2101 gcccggctgt tcggcgcgaa ggacggcgta aacgaagcct tttacgagga actcgaagaa
     2161 gcgctgatcc ttgccgacgt cggcgcggaa gctacggagc gcctgctcga cggcctgcag
     2221 cgggcactgg gcagccaccc cgatgtggtc gc
//
LOCUS       DAOMGY010000258         2250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_53918_length_2250_cov_3.694761, whole genome shotgun sequence.
ACCESSION   DAOMGY010000258 DAOMGY010000000
VERSION     DAOMGY010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53918_length_2250_cov_3.694761"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLR57_04745"
     CDS             complement(<1..395)
                     /locus_tag="PLR57_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40796.1"
                     /translation="MKKEMTYRDSVHRMGRVWILSALALMIAVPVGICVYYSAWPPFS
                     GVLAGFLSVAPIFWTVGVIEVLTYVPMLGTGGSYVGFVTGNLSNLKVPCALNAMERAN
                     LKAGTEEGEVVSTIAISTSAIVTTIIIALG"
     gene            complement(409..1143)
                     /locus_tag="PLR57_04750"
     CDS             complement(409..1143)
                     /locus_tag="PLR57_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HQQ40797.1"
                     /translation="MEFNANSGFMYLLVSIVIVFVILQSVVFLIKAWKHGKEIGMDVS
                     VLKKAAGSAAVFTIAPAVAILLGVIALSKSLGFPLPWLRLSVIGALTYETTAAASAAS
                     AVGVTLGTQITDAKIFSTIVWVMTLGIVPGLVLVPAFGKKIENGLIKIKTKDQRWSEI
                     FMAGLFLGMISAFLGVVFAHVGEGLTGWIPVFVMLASAVLMLLCGLIRKVLNAKWIEE
                     YALPISLLGGMALSIPITTLVQSIAG"
     gene            complement(1456..1830)
                     /locus_tag="PLR57_04755"
     CDS             complement(1456..1830)
                     /locus_tag="PLR57_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HQQ40798.1"
                     /translation="MANVVWKDRKRIIFGLPWTFTRYSLTEEKLTVDTGFLSRKEEEI
                     RLYRILDITLLRPLHQRIWGLGTVRLRTADKSTPEIDIKRIKNAKSFKEMLSDMVEKE
                     RDEKRIAAREFMGADMDDDNLT"
BASE COUNT          517 a          639 c          590 g          504 t
ORIGIN      
        1 cccagcgcaa tgatgatggt ggtgacgatt gcgctcgtgg agatagcgat cgtggacacg
       61 acctcgccct cctcggtgcc cgctttgagg ttggcgcgtt ccatggcgtt aagcgcgcag
      121 ggcaccttca ggttggaaag gttaccggtg acaaacccga cgtaggaacc gcccgtgccg
      181 agcatgggca cataggtgag cacttcgata acgccgaccg tccagaagat cggagctacg
      241 ctcaaaaagc ccgccagaac gccgctgaac ggcggccacg cgctgtagta gacgcaaatg
      301 ccgacgggta cggcaatcat cagcgccagc gcgctgagaa tccaaacgcg gcccatgcgg
      361 tgcacgctgt cgcggtaggt catttctttt ttcatatgct tctcctcctt accctgcgat
      421 cgactgcacg agcgtcgtaa tcggaatcga aagcgccata ccgcccagca ggctgatcgg
      481 cagcgcgtac tcttcgatcc actttgcgtt gagcactttg cggatgagcc cgcagagcaa
      541 catgagcacc gcgcttgcca gcataacgaa caccggaatc cacccggtca gcccttcgcc
      601 gacatgcgcg aacacaacgc ccaggaaggc ggaaatcatg ccgaggaaca gccccgccat
      661 gaagatttcg ctccatctct gatccttggt cttgatcttg atcaggccgt tttcaatctt
      721 cttgccgaac gcgggcacca gcacgaggcc gggcacgatg ccaagcgtca taacccaaac
      781 gatggtcgaa aaaatcttgg cgtccgttat ctgggtgcca agcgttacgc caacggcgga
      841 agccgcgctt gcggcggcag ttgtttcata tgtcagcgcg ccaatgaccg acagccgcag
      901 ccagggcagc gggaagccca gcgattttga cagcgcgatc acgccgagca ggattgccac
      961 cgccggagca atggtaaaca ccgccgccga ccccgccgct ttcttgagca cgctgacgtc
     1021 catgccgatc tcttttccgt gcttccaggc cttgatgaga aacaccacgg actgcagaat
     1081 cacgaacacg atgacgatgg ataccagcag gtacataaac ccgctgtttg cattgaattc
     1141 catcccaatt ctcccttcat tccattccag agttgccgac ggcgcgagcc gagtcccgat
     1201 gcgagtttcc gtttcgcccg tccggatttg gtatatagta tattttgtta ttatagcata
     1261 tcgtgattgc cgaacaagat gttttacaaa tgtatattta ttgacaatag ttgttgaatt
     1321 gcttttgctt ggatattttg attatagcgt ggagacggcg gcgcgataca gaaaacggcg
     1381 gagcgaacgg aggattgtat tccgctcacc ccgccgctga aaacactttt gccgcggcgg
     1441 cagtctgctg atttatcagg ttaagttgtc gtcgtccata tccgcgccca tgaattcgcg
     1501 tgcggcgatg cgcttttcgt cccgctcctt ttcgaccata tcggagagca tttccttgaa
     1561 actcttggca ttcttgatgc gcttgatgtc gatttccggc gtggacttat ccgccgtgcg
     1621 gagacgcacc gtgccgagtc cccaaatacg ctgatggagg ggacgcagga gcgtgatatc
     1681 caaaatgcgg tacaggcgga tttcttcttc cttgcggctt aaaaacccgg tgtcgacagt
     1741 cagcttttct tccgtcaggg aataccgcgt aaacgtccac ggcagcccga aaatgatgcg
     1801 ctttcgatcc ttccaaacga cgtttgccat tgtgcgtcct ccgtatgctt taatagggcg
     1861 attataccat acggcaggct gttacggaag aacggaatcc acgtatctgc cgatgccgca
     1921 aatgcggttt ccctgttgcc tgttctaaaa acactggcga ttgcgccaaa ttttcaaacg
     1981 cggcaaatag tatgctgaca gcacaccaaa agcgttcccg aatccgtaat aatagccttg
     2041 aaagcaatct catttaaaac aggaggccgt gctatgaaac gaatcacaac tctcgttctt
     2101 gctgttatgc tggtctgtat gagcttgtcc ggctgcggcg ttccaaagga cagacccgta
     2161 acagaagtct ggctcggcga taccgctgaa accgttatgc agcttgagct cggactggaa
     2221 aaacagtacg acaggacaaa cacctacctt
//
LOCUS       DAOMGY010000259         2249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_53970_length_2249_cov_3.047858, whole genome shotgun sequence.
ACCESSION   DAOMGY010000259 DAOMGY010000000
VERSION     DAOMGY010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53970_length_2249_cov_3.047858"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1133)
                     /locus_tag="PLR57_04760"
     CDS             complement(3..1133)
                     /locus_tag="PLR57_04760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQQ40799.1"
                     /translation="MNVFLHIDLNVFMVAICLIMFFANRGMVEKRMAQNIIFRLLIVS
                     NIALLILEAITLLLEGHPSPVLIPLYYIVTILLYILTPIPALLWLMYVNWQLFQNSRK
                     LKTSVIIFGIPAAVCMILTVLSPLNGWMFTIDANNIYQRGVLYPLLAFTCFFPTIFAA
                     LLLAINRKRISKKMLVLMLLFTVPPLLGAVAQVLFYGITVLWSSITISIFFIHTNIQS
                     TQIHVDHLTGAFNRRQLDVILEDRIHTAQSKQPLSCILLDIDHFKAINDTLGHIEGDE
                     ALKDASALLQSCIRKRDLLARFGGDEFIIITDIDNDKTLGELCQRIRQAVEGFNQSCH
                     RNYTLGFSIGSAVYDPDSGWSKDRYIAHVDSLMYLDKNGGRK"
     gene            1341..1886
                     /locus_tag="PLR57_04765"
     CDS             1341..1886
                     /locus_tag="PLR57_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ40800.1"
                     /translation="MQTLESERLILRGFTELDTDDLFRYAADPDVGPRAGWKPHASRA
                     ESLAIVRMFVNDDNVWAIERKSDHRVIGSLGLHADKLRNLPDVKTFGYVLAQDCWGNG
                     YMTEAVRRAMEYTFTEAGMRLMSVSHYTFNDRSRRVIEKCGFTCEGTIRSAFQRFDGE
                     VFDETVYSITKEEWEKQSKQW"
     gene            1941..>2249
                     /locus_tag="PLR57_04770"
     CDS             1941..>2249
                     /locus_tag="PLR57_04770"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HQQ40801.1"
                     /translation="MAEQKRIVIESRSNERVKDARRLLQRKERKESGLHLIESEKLVR
                     EAIASGVRISALFVEDGFAFPVPENVPVYAVSRSVMESLCESQSPQGVVAVVETPSLL
                     P"
BASE COUNT          560 a          582 c          595 g          512 t
ORIGIN      
        1 tctcattttc tcccgccgtt tttatccaga tacatcagcg aatcgacatg cgcaatatag
       61 cggtccttgc tccagcctga gtcgggatcg tacaccgcgg aaccgatgct gaatccaagc
      121 gtgtagtttc gatgacagct ttggttaaag ccttccaccg cctgcctgat ccgctggcag
      181 agttcaccaa gcgtcttgtc gttgtcgata tccgtgatga taatgaactc gtccccgcca
      241 aagcgcgcca gcaggtccct ttttcggatg cagctttgca gcagtgccga agcgtccttg
      301 agtgcttcgt ctccctcgat atggccgagc gtatcgttga tcgccttaaa gtggtcgata
      361 tcgagcagaa tgcaggaaag tggttgtttg ctttgcgccg tgtgaatccg gtcttccaaa
      421 attacgtcca attgcctgcg gttgaacgcg ccggtcaggt ggtctacatg aatttgagta
      481 ctttgaatgt tggtgtgaat aaaaaagatt gagatagtaa tgctgctcca caagacggta
      541 atgccataaa acagcacctg cgccaccgcg cccagaagcg gaggcacggt aaacaaaagc
      601 atcaagacca gcattttctt tgagatgcgc ttgcggttga ttgccagcag cagcgctgca
      661 aaaatggttg gaaagaaaca tgtgaatgct agcagcgggt acagtacgcc tcgctggtag
      721 atgttgtttg cgtcaatggt aaacatccag ccgttcagcg gcgacagcac ggtcaaaatc
      781 atgcatactg cggcaggtat gccaaatata atcaccgatg ttttaagttt gcgcgagttt
      841 tggaacaatt gccagtttac atacatcagc cacagcagcg cgggtatcgg tgtcagtatg
      901 tagagcagta tcgtaacaat gtaatagaga ggaatcaata cgggcgaggg atgcccctcc
      961 agaagcaatg tgatagcctc cagaattagc agcgcgatgt tcgaaacgat cagcagccga
     1021 aaaatgatat tttgcgccat tcgtttttcg accatgccac ggtttgcaaa gaacataatc
     1081 aagcaaatcg caaccatgaa cacattcaaa tcaatatgca agaaaacgtt cataagtttt
     1141 ccttcggcaa tatgatgagg aatcaataga tatttgcatc atatcttgat ccaccaaagc
     1201 tgtcaacgcc cggcatattt ttttattgcg cgaaccccgc atgacacggc gctttttcaa
     1261 aacgtcccaa accgtgccgc tccccttatt cttgccgttg aagtgtgtta tactgtatga
     1321 aaaatatccg gagattccaa atgcaaactc ttgaatccga acgcctgatc ctgcgcggct
     1381 ttaccgagct ggacaccgac gacctctttc gctatgccgc cgatcccgac gttggtcctc
     1441 gcgcggggtg gaagccgcac gccagccgcg cggagtcgct cgcgattgtc cgcatgttcg
     1501 taaacgacga caacgtttgg gcgatcgagc gcaaaagcga tcaccgcgtg atcggctcgc
     1561 tcggcctgca cgcggataaa ctgcgcaacc tgccggatgt aaagacgttc ggctacgtgc
     1621 tcgcgcagga ctgctgggga aacgggtata tgacggaagc cgtccgccgc gcaatggagt
     1681 atacattcac cgaggcgggc atgcgtttga tgagcgtttc acactacacc ttcaacgacc
     1741 gttcgcgccg ggtcatcgaa aagtgcggct tcacctgcga aggcaccatt cgcagcgcgt
     1801 ttcagcgttt cgacggcgaa gtgtttgacg aaacggtcta ttccattacc aaagaagaat
     1861 gggaaaaaca atcgaagcag tggtaaccgc ctgctttttc acctgggata cagtaacgtc
     1921 gaataggagg cgaagcgcgc gtggcagaac aaaagcgaat cgtcatagaa agccgctcca
     1981 acgagcgcgt caaggacgcg cgcagactcc tgcagcgcaa ggagcggaag gaaagcggcc
     2041 tgcacctgat tgaaagcgaa aaactcgtgc gggaagcgat tgcttccggc gtacgaattt
     2101 ccgcgttgtt cgtcgaggac gggtttgcgt tccccgtgcc ggaaaatgtt ccggtatacg
     2161 ccgtttcgcg ttcagtaatg gagtccctct gcgagagcca gtcgccgcag ggcgttgtag
     2221 cggtggtgga aacgccctcg ctccttcca
//
LOCUS       DAOMGY010000260         2245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_54126_length_2245_cov_3.226941, whole genome shotgun sequence.
ACCESSION   DAOMGY010000260 DAOMGY010000000
VERSION     DAOMGY010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54126_length_2245_cov_3.226941"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLR57_04775"
     CDS             <1..192
                     /locus_tag="PLR57_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40802.1"
                     /translation="KRPETFAEELLGEETLPEEETLLEHERAVENAPRQIGRRRQKTK
                     DKEAEEPANDEDPFDKIVD"
     gene            complement(286..1113)
                     /locus_tag="PLR57_04780"
     CDS             complement(286..1113)
                     /locus_tag="PLR57_04780"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HQQ40803.1"
                     /translation="MEQTRPNADELKDETRSAKVLAEAEQRQKAHRPPSPATEDRPRH
                     RVSSRTIRWLLRLILFVVLVIALLILYAKLKDFLTGGITITAPESLSALLPDETMGYS
                     RFDFSNAILGESREKSEFVVLEQDVSVTSRVSQALANLALFEKSQLIRSYGTGVYSVD
                     LSKLTEDEVALDNELKTVTVTIPHAALSYLTVDVDKTEYEETQKAIFAFGEIKLTNEQ
                     MGLLEQSIQDAMRAELETADMLAKADASAITQVQKLFLPLVQAVAADYTVVILLADS"
     gene            1453..>2245
                     /locus_tag="PLR57_04785"
     CDS             1453..>2245
                     /locus_tag="PLR57_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQQ40804.1"
                     /translation="MSKIVFDDHTNLLRENYYEYEVQDVPEANLFPEIFTFDEIPKVA
                     FNQRLVPMHMPKDVWITDTTFRDGQQSRAPFTSAQILKLYDLMHDLGGPHGMIRQCEF
                     FVYSAQDRQVLEMCRTRGYRFPEITTWIRATESDFNLVKELKIRETGILVSCSDYHIF
                     KKMNLTRSQAMEKYLSVVKMALDRGITPRCHFEDITRADFYGFVVPFAHALQRLSEES
                     GIPIKIRCCDTLGLGVSYPGASLPRSVPGIIYGLRHYANVPSSLIE"
BASE COUNT          511 a          599 c          626 g          509 t
ORIGIN      
        1 aagcgccctg agacgtttgc cgaagagctt ttgggtgaag aaactctgcc ggaagaagaa
       61 acgcttctgg agcacgagag agccgttgag aacgcgccgc gccagatcgg acgcagacgc
      121 cagaaaacga aggacaagga agcggaagaa ccggccaacg acgaagatcc gtttgataaa
      181 atcgtagact gaaaagcccg ttcaaaaaca caaaaatacg cccgccggaa cctatagggt
      241 accggcgggc gattgttatc tgcgtggatg ctttccgctt tcagcttacg agtcggccag
      301 caggatgacg accgtgtaat cggcggctac ggcctgcacg agcggcaaaa agagcttctg
      361 cacctgtgtt atcgccgaag catccgcttt tgcaagcata tccgccgttt ccagctcggc
      421 gcgcatcgcg tcctgaatgc tctgctccag cagacccatc tgctcgttgg tcagcttgat
      481 ttcgccaaag gcgaagatcg ctttttgcgt ttcttcgtac tcggttttgt ccacgtccac
      541 ggtcaggtag gaaagcgcgg cgtgcggaat ggtcacggtg acggttttca gctcgttgtc
      601 gagcgcaact tcgtcctccg tgagcttcga gagatccacg gagtaaacgc cggtgccgta
      661 ggagcggatg agctggctct tttcaaatag cgcgaggttt gccagtgcct gactgacgcg
      721 gctggtcacg gaaacatcct gctccagcac gacaaactcg ctcttttcac gcgattcgcc
      781 taagatggcg ttggaaaaat cgaaccgact gtagcccatg gtttcatccg gcagcagggc
      841 ggaaagcgac tccggcgctg taatggtgat gccgccggtc aggaaatcct tgagcttggc
      901 atagagaatt aaaagcgcaa tgacgagaac gacaaacaga atcaaccgca aaagccatcg
      961 tatggtgcgg ctgcttactc tgtggcgcgg gcggtcttct gtggcggggg atgggggacg
     1021 gtgcgctttc tgccgttgtt cggcttcggc aagcaccttg gcggaacggg tttcgtcctt
     1081 gagttcatcc gcgttcgggc gggtctgttc catgttcctg cgcctccgtt tctccgcacg
     1141 cgcggttcat gctctatgaa ttcagtatac gtacgcgggg aaacgcctgt caaccggcgg
     1201 ctgactttga cacgtactgt ttgcggcgcg ttcaagtttt tcctttcgat tgcactgttt
     1261 atgcagaaaa aatacgattc acttgacaga cttgcggcag aagaataaac tactctgaaa
     1321 ttatatgcaa aatatgcgta taatcttcgc attttcccgt ttttcacctt gacaccggtt
     1381 ttgaggtgct gttataatga acagcatatt gattgtatac aagcatgcaa gcatgccgaa
     1441 aggaagggaa caatgagcaa aatcgttttt gacgaccaca caaacttgct gcgcgagaac
     1501 tactatgagt acgaagtaca ggatgtgccg gaagcgaatc tgttcccgga aattttcacg
     1561 ttcgacgaaa tccccaaggt tgcgtttaac cagcggctgg tgccgatgca tatgcccaag
     1621 gatgtttgga ttaccgatac gaccttccgc gacggacagc agtcccgcgc gccgtttacg
     1681 agcgcgcaga ttctcaagct gtacgatttg atgcacgacc taggcggccc gcacggaatg
     1741 atccgtcagt gcgagttttt cgtttacagc gcgcaggatc gtcaggtact ggaaatgtgc
     1801 cgtacgcgcg gctaccgctt cccggagatt acgacctgga ttcgcgccac ggagtcggac
     1861 tttaacctcg tcaaagagct gaaaatccgc gagacgggca tcctcgtttc ctgctcggat
     1921 taccacatct tcaaaaagat gaacctcacg cgctcgcagg cgatggagaa atacctcagc
     1981 gtcgtcaaga tggcgctgga tcgcggcatt acgccccgct gccactttga ggacatcacc
     2041 cgcgcggact tctacggctt tgtggtgccg tttgcgcacg cactgcagcg cctctccgag
     2101 gagagcggta ttcccatcaa gattcgctgc tgcgacacgc tgggtctcgg cgtttcgtat
     2161 cccggcgcga gcttgccaag aagcgtacca ggcatcattt acggcttaag gcactatgcc
     2221 aacgtgccga gttcgctgat tgaat
//
LOCUS       DAOMGY010000261         2236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_54468_length_2236_cov_3.113709, whole genome shotgun sequence.
ACCESSION   DAOMGY010000261 DAOMGY010000000
VERSION     DAOMGY010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54468_length_2236_cov_3.113709"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..415
                     /locus_tag="PLR57_04790"
     CDS             50..415
                     /locus_tag="PLR57_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40805.1"
                     /translation="MDMQAKKIILGNHEHDAPGGLFDVPRWGKVMRPILEKNPNVSVI
                     NSNLDVSAYPEYAGLTKPYVIEEVMAPWGKTKVATISKAQVREIAELKMPDLNAANVE
                     TAMSMIAGTARSMGVIVAE"
     gene            430..1119
                     /gene="rplA"
                     /locus_tag="PLR57_04795"
     CDS             430..1119
                     /gene="rplA"
                     /locus_tag="PLR57_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HQQ40806.1"
                     /translation="MKHGKKYLESKKSIEAQKQYDVHEGLELVLTTAKAKFDETIEAS
                     VRLGVDPRHADQQVRGAVVLPHGTGKKVRVLVFAKADKAREAQEAGADYVGDEDLVKK
                     IQTENWFEFDVCVATPDMMGVVGRIGRVLGPKGLMPNPKSGTVTMDVAKAVQDIKAGK
                     VEYRVDKTSIVHCPIGKASFGVEKLEENLTTLMDAIVKAKPSAAKGTYLKSVVISATM
                     GPGVRFNTLKF"
     misc_feature    1230..1370
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1402..1920
                     /gene="rplJ"
                     /locus_tag="PLR57_04800"
     CDS             1402..1920
                     /gene="rplJ"
                     /locus_tag="PLR57_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQQ40807.1"
                     /translation="MANVQNIERKAEQVALVAEKVKKAQSVVVFDYRGISVAEDTELR
                     SEMRKNGVEYVVIKNHIMARACEASGVHEHVEEILKGPSAFAFGYEDVAAPARILKAF
                     IKKAKKCELKGGIVECSVLDAKGIEAIADMPSREVLIARMLGSMMSPISKFAIVLDQI
                     AKKQGGTDEKAE"
     gene            1984..>2236
                     /gene="rplL"
                     /locus_tag="PLR57_04805"
     CDS             1984..>2236
                     /gene="rplL"
                     /locus_tag="PLR57_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQQ40808.1"
                     /translation="MDKEKLIAEIKAMSVLELFEIVKALEEEFGVSAAATAVAVAGPA
                     AAAEEVEEKTEFDVILKEVGPEKIKVIKVVRELTGLGLKE"
BASE COUNT          585 a          584 c          633 g          434 t
ORIGIN      
        1 cgtaaatttc gctttcacta aaggcggcaa agtcgaatac gaaagtctca tcgatatgca
       61 agccaaaaag attattttag gcaaccacga acatgacgct ccgggcggac ttttcgacgt
      121 tccccgctgg ggcaaagtaa tgcgcccgat tctcgaaaaa aatccaaatg tttcggtcat
      181 aaactcaaac ctggacgtca gcgcttatcc tgaatatgcc ggtttgacca agccctatgt
      241 cattgaagaa gtgatggcac catggggcaa aaccaaagtc gccaccattt ccaaggcgca
      301 ggtgcgtgag attgccgagc tgaaaatgcc cgacctcaac gccgccaacg tggaaacggc
      361 tatgtccatg atcgccggta ccgcccgcag catgggcgtt atcgtcgctg agtaaggggg
      421 agacgagcga tgaaacacgg caaaaagtat ctcgaaagca agaagagcat cgaagcccag
      481 aagcagtacg acgtacacga agggcttgaa ctcgttctga ccaccgcaaa ggcgaagttc
      541 gacgaaacta tcgaagcttc cgtccgtctg ggcgttgacc cgcgccatgc cgaccagcag
      601 gtccgcggcg ccgtcgttct gccgcacggc acgggcaaaa aggttcgcgt tctcgtgttc
      661 gccaaggccg acaaagcgcg tgaagcgcag gaagccggcg cggattacgt tggcgacgaa
      721 gacctcgtca agaagatcca gaccgaaaac tggtttgagt tcgacgtctg cgttgcgact
      781 ccggacatga tgggcgtcgt cggccgtatc ggccgcgtgc tcggccccaa aggcctcatg
      841 ccgaacccga agtccggcac ggtcaccatg gacgtcgcca aagccgttca ggacatcaaa
      901 gccggtaagg ttgagtaccg cgtggacaag accagcatcg tgcactgccc gatcggcaag
      961 gcttccttcg gcgttgaaaa gctcgaagaa aacctcacca cgctgatgga cgcgatcgta
     1021 aaggccaagc cgtccgcggc gaagggcacc tacctcaaga gcgttgtcat cagcgccacg
     1081 atgggcccgg gcgtccgctt caacaccctc aagttctaac gaatcgtctt gggaaagcgg
     1141 ttttcagccg ctttcccggg atttttcaag cattgatcca cacgaatcgc ttgttttcat
     1201 tgacaacgcg caaaagagtg tggtatcatt cttcccggta acgatcgccc aagacagccg
     1261 gcagcgggga accgcataag tcctgccgag gtgaagcgtg caacgattca ttttgtttgt
     1321 atgcaatcct cctgtcttgt ttcaaggcgg gaggattttt agttttccaa tgtggtattg
     1381 caaggaggtg aaacaacaaa tatggcaaac gttcagaata tcgaacgcaa agcggagcag
     1441 gttgccctcg tcgcggaaaa ggtgaagaag gcgcaaagcg ttgtggtgtt tgattatcgc
     1501 ggtatttctg ttgcagagga caccgaactg cgctcggaaa tgcgcaaaaa cggcgtggaa
     1561 tacgtggtta tcaagaacca tatcatggct cgtgcctgtg aagcgagcgg cgtgcacgaa
     1621 cacgttgaag aaattctcaa agggccgagc gcattcgcgt ttggctacga agacgttgcg
     1681 gctccggcgc gcatcctcaa ggcgttcatc aagaaggcca agaagtgcga gctcaagggc
     1741 ggtatcgtcg aatgcagcgt gcttgacgca aaggggatcg aagcgatcgc cgacatgccg
     1801 agccgcgagg tgctgattgc gcggatgctg ggctccatga tgtccccgat ttccaagttc
     1861 gcaatcgttc tcgatcagat cgccaagaaa cagggcggta cggacgaaaa ggcggaataa
     1921 cccgcaaaca aaaaacaaca aaaattgcgg gcaatccgca gaaaaaatta aatggaggat
     1981 attatggata aggaaaaatt aatcgctgag attaaagcga tgtccgttct cgaactgttc
     2041 gagatcgtta aggctcttga agaggaattc ggcgtttccg cagccgcgac cgcagtcgcc
     2101 gttgccggcc ccgccgccgc tgctgaagaa gtcgaagaga agaccgaatt cgacgtcatt
     2161 ctcaaggaag tcggccccga gaagatcaag gtcatcaagg tcgttcgtga gctgaccggt
     2221 ctgggcctca aagaag
//
LOCUS       DAOMGY010000262         2222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_54995_length_2222_cov_3.476696, whole genome shotgun sequence.
ACCESSION   DAOMGY010000262 DAOMGY010000000
VERSION     DAOMGY010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54995_length_2222_cov_3.476696"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLR57_04810"
     CDS             <1..552
                     /locus_tag="PLR57_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HQQ40809.1"
                     /translation="QAGAEAAFCGRVPDDADMLNQKVSACCGECDILLLSGGSSVGVK
                     DAAANCVSSLGELLFHGLAVKPGKPAFAGTIGKTLVLGLPGHPAAAYFVFELLARPGI
                     YALLGQTAREFTVQAKLTAAVPSNTGREEYVAVRLEEGTAEPLMSKSGLISVLSRADG
                     YIRIPRDAEGFAKGASVKVHLFC"
     gene            604..>2222
                     /locus_tag="PLR57_04815"
     CDS             604..>2222
                     /locus_tag="PLR57_04815"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HQQ40810.1"
                     /translation="MEFEYLSNTPLQEAVRGYLSFLKTNGMTYRTEMIASPDALDRVT
                     ACAVYANLCAPHYTACAMDGIALMAKRTFGASETTPVLLETNDYVRVDTGDPLPEGCD
                     AVVMMEDCVERDGKIALYAAAAPWQHVRQIGEDIATGDMILPSFRQIEPAMIGAMLAG
                     GVLSLEAVCRPKVGIIPTGDEIVPPSANPREGEVMEFNSAIFSGMLRRWGAEPRTYPI
                     VPDDPERIRAALKTAADECDLVLLCAGTSAGRDDGTVSAISALGEVYLHGVAIRPGKP
                     VVLGRVGAKPVYGVPGYPVSGIVVLEELVRPVIDALLMREREAGETVCVRMGRRLNSS
                     LKYREFVRATLGLGAEGQLSAVPLDRGAGVVTSFVKADCLLDVPQNCEGYEAGDRAFA
                     RLLKPVERIARTVRILGSHDPLIDEAADELRRMDISAHVSSAHVGSMGAILALSRGEA
                     QLGGVHLLDETDGSYNVSYIEKYFPNGGVALIECVRRTQGLMVKKGNPKGIQGIADLA
                     RGDVSYVNRQKGAGTRVLLDYLLRQEGVASA"
BASE COUNT          408 a          630 c          747 g          437 t
ORIGIN      
        1 caggcgggag cggaagcggc gttttgcgga cgcgtgccgg acgacgcgga catgctaaac
       61 caaaaggttt cagcctgctg cggcgagtgt gatattttat tgctttccgg cggtagctcg
      121 gtgggggtaa aggacgcggc ggcaaactgc gtttccagcc tcggcgaact gctgtttcac
      181 ggcctcgcgg tcaaacccgg caagcctgcg tttgctggaa cgattggaaa aacgctcgta
      241 ctcggtctgc cggggcatcc ggctgcggcg tacttcgtgt tcgaactgct cgcgcggccc
      301 ggaatctatg ccctgcttgg ccagaccgcg cgtgagttta ccgtacaggc aaaactgacc
      361 gcggcggtgc cttccaacac tggacgggag gagtatgttg cagtgcggct ggaagaaggc
      421 acggcggaac cgctgatgag caaatccggt ctgatttccg tgctctcccg cgcggacggc
      481 tatatccgca ttccgcgtga cgcggagggc tttgccaaag gcgcaagcgt taaggtgcat
      541 cttttttgtt gatggtttta cgtcattcaa aatctcgcaa tctatggaaa agagctgaaa
      601 acaatggagt ttgaatattt gagcaacacc ccgctgcagg aagctgtgcg cgggtatctt
      661 tctttcttaa agacgaacgg tatgacctac cggacggaga tgatcgcctc tcccgacgcg
      721 ctcgaccgcg tgacggcttg cgcggtttac gcaaaccttt gcgcgccgca ctacaccgcc
      781 tgcgcgatgg acggcattgc gcttatggca aagcgtacgt tcggcgcaag cgagaccacg
      841 ccggttctgc tggaaacgaa cgactacgtc cgcgtggaca ccggcgaccc actgccggaa
      901 ggctgcgacg cggttgtgat gatggaagac tgcgtcgagc gggacggaaa gatcgcgctc
      961 tatgcggcgg cggcgccctg gcagcacgtg cgccagatcg gcgaggatat cgccacgggt
     1021 gatatgattc tgccgtcgtt tcgccaaatt gagccggcta tgatcggcgc aatgctcgcg
     1081 ggcggcgtac tttctctgga agcggtctgc aggcctaagg tcggcattat cccgacgggg
     1141 gacgaaatcg ttccgcccag cgcgaacccg cgcgagggag aagtgatgga gttcaactcc
     1201 gccatctttt ccggcatgct gcgccgctgg ggcgcggaac cgcgcacgta tccaatcgta
     1261 ccggatgacc ccgaacggat tcgagctgcg ctcaaaacgg cggcggacga atgcgacttg
     1321 gttctgctct gtgcgggaac ttccgccggg cgggacgacg gcaccgtttc cgcgatttct
     1381 gcgcttggcg aagtctatct gcacggcgtt gcgattcgtc cgggtaagcc agtcgtgctg
     1441 gggcgcgtcg gcgcaaagcc ggtgtatggc gtgccgggat atcccgtgag cggcatcgtc
     1501 gtgctcgaag agctggtgcg tcctgtgatc gacgcgctgc tcatgcgcga gcgggaagcc
     1561 ggcgaaacgg tctgcgtgcg catggggcgg cggctcaact cgtcgctgaa ataccgcgag
     1621 tttgtgcgcg ctactcttgg cttgggcgcg gaaggtcagc tctccgccgt accgcttgat
     1681 cggggagcgg gcgttgtgac ctcgtttgtc aaggcggact gcctgctgga tgttccgcaa
     1741 aactgcgaag ggtacgaagc gggcgaccgc gcgtttgcgc gcctgctcaa gcccgtggaa
     1801 cgcattgcgc gcacggtgcg cattctcggc agccacgacc cgctcataga cgaagcggcg
     1861 gacgaactgc ggcggatgga catttccgcg catgtttcca gcgcgcatgt cggcagcatg
     1921 ggggcgattc tcgcgctctc gcgcggggaa gcgcagctcg gcggcgtgca tttgctggac
     1981 gagacggacg gaagctacaa tgtttcttat atagagaagt atttccccaa tggtggcgtg
     2041 gcgctcatcg aatgcgtccg gcgcacgcag gggctgatgg tcaagaaggg caacccgaaa
     2101 gggattcaag gaatcgccga tctcgcgcgg ggcgacgttt cctacgtcaa ccgccagaag
     2161 ggcgcgggca cgcgcgtgct gctggattat ctgctccggc aggagggcgt tgcttccgcg
     2221 ca
//
LOCUS       DAOMGY010000263         2220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_55085_length_2220_cov_3.213857, whole genome shotgun sequence.
ACCESSION   DAOMGY010000263 DAOMGY010000000
VERSION     DAOMGY010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55085_length_2220_cov_3.213857"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..2107
                     /gene="thrS"
                     /locus_tag="PLR57_04820"
     CDS             158..2107
                     /gene="thrS"
                     /locus_tag="PLR57_04820"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQQ40811.1"
                     /translation="MFITFPDGNKREFKDGSTGIEIAAEISPRLAKATLSCDIDGAHS
                     DAFRPITGDHAIKFYTFSDENGRWAYRHTASHVLAQAVKRLYPEVKLAIGPAIENGFY
                     YDFDAPSAFTPEDLVKIEAEMARIEKEDLKIERFELPRAEAIKFMEQKGEPYKVELIR
                     DLPEDAVISFYREGDFVDLCAGPHVPSTGIVKNIKLLNVAGAYWRGSEKNKMLQRIYG
                     TAFEKKADLDAYVTALEEAKKRDHNKLGRELELFTTVDIIGQGLPILLPKGARVIQLL
                     QRFVEDEEQKRGYNLTKTPFMAKRELYKISGHWDHYRDGMFIMSDTPDVEDENAEVFA
                     LRPMTCPFQFMAYLNRARSYRDLPLRYNETSTLFRNESSGEMHGLIRLRQFTISEGHL
                     ACRPDQVEDEFKGCLDLAKFMIDTLGFTDDVSYRFSKWDENDTAKYIGDKASWESTQN
                     MMRTILNDLGVQFTEADGEAAFYGPKLDIQIRNVYGKEDTLITIQIDFQLAERFGMVY
                     TDTDGQKKFPYVIHRTSIGCYERTLALLIEKYAGALPTWIAPVQVKVLPITDRAADAS
                     VEIQKSLEQYGIRVESDLRNEKIGFKIREAQMQKVPYMLVMGDKEMEQGLVAVRSRKD
                     GDLGTMTLEAFRAKLLEEVATRAKS"
BASE COUNT          551 a          639 c          643 g          387 t
ORIGIN      
        1 gcgcgcgcag gacaaaaacc accgctgtgc gaacgcgggt ggaaccacgg tagcaaggaa
       61 ctatcgtccc gaggcagaat aatctgcttc ggggcgtttt gttttatccg gcaaaaagcc
      121 ggaacaggct atgaaccaaa acaggaggag aagaagtatg tttattacat tcccggacgg
      181 gaacaaacgc gaattcaagg acggcagcac ggggatcgag atcgccgcgg agatcagtcc
      241 gcgccttgcc aaggccacgc tgagctgcga cattgacggc gcgcattccg acgcgttccg
      301 cccgatcaca ggcgaccacg cgatcaagtt ttatactttt tcggacgaaa acggccgctg
      361 ggcttaccgc cataccgcgt cccacgtgct ggcgcaagcg gtcaagcggc tgtacccgga
      421 ggtcaagctc gccatcggcc ccgcgattga aaacgggttc tattacgact ttgacgcgcc
      481 gagcgcgttc acgccggaag acctcgttaa aatcgaagcc gaaatggcgc gcatcgaaaa
      541 agaagatctc aagatcgagc gcttcgaact gccgcgcgct gaagcaatca agttcatgga
      601 gcaaaaaggc gagccgtaca aggtggagct catccgcgac ctgccggagg acgccgtgat
      661 cagcttctac cgcgaaggcg acttcgtcga cctctgcgcg gggcctcacg tgccttccac
      721 ggggattgtc aagaacatca agctcctcaa cgttgcgggc gcttactggc gcggcagcga
      781 gaagaacaag atgctccagc gcatctacgg cacggcgttt gaaaagaaag ccgacctgga
      841 cgcgtatgtg accgcgctcg aagaagcgaa aaagcgcgac cacaacaagc tcggccgcga
      901 gctggagctg tttacgaccg tggacatcat cggtcagggt ctgcccatcc tactgcccaa
      961 gggcgcgcgg gtcattcagc ttctgcagcg gttcgtcgag gacgaagagc agaaacgcgg
     1021 ctacaacctc accaagacgc cgtttatggc caagcgtgag ctctataaaa tctccggtca
     1081 ctgggatcac taccgcgacg gcatgttcat catgagcgac acgccggacg ttgaggacga
     1141 aaacgcggaa gtcttcgcgt tgcggccaat gacctgcccg ttccagttca tggcatacct
     1201 caaccgcgct cgttcctacc gcgatctgcc gctgcgctat aacgaaacga gcacgctgtt
     1261 ccgcaacgaa agctccggcg aaatgcacgg cctgattcgt ttgcgtcagt tcaccatttc
     1321 ggaagggcat cttgcgtgcc gtccggatca ggtggaagat gagttcaagg gctgtctcga
     1381 tctggcgaag ttcatgatcg acaccctcgg ctttacggac gacgtgagct accgcttcag
     1441 caaatgggac gagaacgaca ccgccaagta cattggcgac aaggcgagct gggaaagcac
     1501 gcagaacatg atgcgcacca tcctcaacga cctcggcgtg cagtttacgg aagcggacgg
     1561 cgaagcggcc ttctacgggc cgaagctcga catccagatt cgcaacgtct acggcaaaga
     1621 ggacacgctc atcaccatcc aaatcgactt ccagcttgct gagcgcttcg gcatggtgta
     1681 tacggacacg gacggacaga agaagttccc ttacgtcatc caccgcacgt ccatcggctg
     1741 ctacgagcgc acgctggcgc tgttaatcga aaagtacgcc ggcgcgctgc ccacgtggat
     1801 tgcgcccgta caggtgaagg tgctgcccat caccgaccgc gcggcggacg cttccgtcga
     1861 gattcagaag tcgctggagc agtacggcat ccgcgtggaa agcgacctcc gcaacgagaa
     1921 gatcggcttc aaaatccgcg aggcgcagat gcagaaggtg ccttatatgc tcgtgatggg
     1981 cgataaggaa atggagcagg gactcgtcgc cgtgcggtcc cgcaaggacg gcgacctcgg
     2041 caccatgacg ctggaggcct tccgcgccaa actgctcgaa gaagtcgcaa cgagggcgaa
     2101 gtcgtaacaa gaacaacgca agtacaaaaa agagggcgcg aaagcgccct ctttttatat
     2161 ttctaaggtg aattatacta tcaagtgcaa cactttaggg aaataaagaa gttcatgcaa
//
LOCUS       DAOMGY010000264         2217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_55217_length_2217_cov_2.564755, whole genome shotgun sequence.
ACCESSION   DAOMGY010000264 DAOMGY010000000
VERSION     DAOMGY010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55217_length_2217_cov_2.564755"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLR57_04825"
     CDS             complement(<1..289)
                     /locus_tag="PLR57_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQQ40812.1"
                     /translation="MKLSTRSKYGLKAVVDLAIRYGSGPVSLPTLAGEQGISEGYLEQ
                     LLRELKKGGVVDTVRGAQGGYELVCAPSELSVQRVLSLLEGSTAVVDCVDID"
     gene            complement(414..914)
                     /locus_tag="PLR57_04830"
     CDS             complement(414..914)
                     /locus_tag="PLR57_04830"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQQ40813.1"
                     /translation="MKQSRVSEPNPGASIRAASPVGELIIHSNGRAVCAITNAQPEDQ
                     SVPTQAQGHPELTQAVKELGEYFAGKRTVFTFPMEPDGTPFQKSVWDALLKIPYGKTA
                     TYGQIAMAVGNPKGARAVGMACNKNPIWIAVPCHRVIGANGSLTGYALGMGMKQQLLT
                     LEGAIK"
     gene            1008..1535
                     /locus_tag="PLR57_04835"
     CDS             1008..1535
                     /locus_tag="PLR57_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40814.1"
                     /translation="MEEAREELALEPAQPQTAQPEEKFETIDYVRELFLNSEEQKKTD
                     HKRVRLLRACVILLSVIAFCIVAATAVLVPTALITAKEVNDSLAVVQKIDVDALVTTV
                     NDFTDEAGNTFTSVGNAVSVLEELDIDTLNETIAQLKTAVESFSQVDITKLNEAIENL
                     NDSVEPFANFFNKFN"
     gene            1621..>2217
                     /locus_tag="PLR57_04840"
     CDS             1621..>2217
                     /locus_tag="PLR57_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40815.1"
                     /translation="MAELFSILYLALFALSGLGISRLFFYRDRPMIRIWLGLTFGMLM
                     LIWLPALFSFVFGFVLLSQLLGLFAACLIGAACWLLSRKKSLTAAPWKKELPALITCG
                     ALFLFCLVLLSSHTIVERDGALYVGQSTYGDLAMHLGFMTSIAAQETFPPMYSICPGT
                     PVGYPFLSDSVSATFLKLGASLRFAAILPAAFAFVIVIV"
BASE COUNT          433 a          650 c          581 g          553 t
ORIGIN      
        1 agtcgatatc cacgcagtct accaccgcag tggaaccttc cagcaggctc agcacgcgct
       61 gaacggagag ttccgagggg gcgcagacca gttcgtatcc accctgcgcg ccgcggacgg
      121 tatccaccac gccgcctttt ttaagttccc gcagaagctg ctccaaatat ccctcggaga
      181 tgccctgctc tcccgcgagc gtcggcagcg acacaggccc cgatccatac cggatggcga
      241 ggtctacaac cgctttcaag ccgtatttgc ttctcgtgga aagtttcatt tgcgcctcat
      301 accctagtaa attactcgtg ttacgtgcaa agcatagcat ttgagtagga tattgtcaag
      361 ggtttgcctt gtgaattttt acgtacgaag tgtgtgcgaa aaatgcgatg cttttattta
      421 atcgcacctt ccagcgtaag cagctgctgc ttcatgccca taccgagcgc ataacccgtg
      481 agcgaaccgt tcgcgccgat tacgcgatgg cacggcaccg caatccagat cgggtttttg
      541 ttgcaggcca tgcctacggc gcgcgcccct ttggggttcc ccaccgccat ggcgatctga
      601 ccgtaggtcg cagttttgcc gtacggaatc tttaacagcg cgtcccaaac ggacttctga
      661 aatggcgtgc cgtccggctc catgggaaac gtaaacacgg tacgcttgcc cgcaaagtat
      721 tcgccgagtt ccttgaccgc ctgcgttagt tccggatgtc cttgcgcttg cgttggaacg
      781 ctctgatctt ccggctgcgc gttcgtaata gcgcataccg cgcgcccgtt ggaatggatg
      841 atcagctcgc cgaccggaga ggctgcgcga atgcttgcgc cggggtttgg ttcgctcact
      901 ctgctttgtt tcatcgggtc gatcctcctg ccgcgcgggc tgtttctttt tattataccc
      961 gatctgtggt atactgacaa aaaaatacgg aaaggatttt ttagcagatg gaagaagcgc
     1021 gcgaagaact cgccctcgaa ccggcacagc cacagactgc gcagccggaa gagaaatttg
     1081 aaaccatcga ctatgtgcgc gagctgtttc tcaactccga ggagcagaaa aaaaccgacc
     1141 ataaacgcgt gcggcttctc cgcgcctgcg tgattctgct ttccgttatc gcgttctgca
     1201 ttgttgcggc aacggcggtt ctcgtgccaa ccgcgctcat caccgcaaag gaagttaacg
     1261 attctctcgc cgttgtgcag aagatcgacg tggacgcgct tgtcaccacg gtaaacgact
     1321 ttaccgacga agccggtaac acttttactt ccgtcggcaa cgcggtttcc gtgctggaag
     1381 agctggacat cgatacgctc aacgagacga tcgctcaact gaaaaccgcc gtagaatctt
     1441 tctcgcaggt ggacattaca aagctcaacg aagcgattga aaaccttaac gactcggtcg
     1501 agccgtttgc gaacttcttc aacaagttta actaatccga cgggcggcgc gatttcggga
     1561 agcactcccc tgcccgccgt ctttgcccgc gcggcaacat aaaaggagtc ttgactgcat
     1621 atggcggaac tgttttcgat tctgtacctt gccctgttcg ctctttccgg cctcggaata
     1681 tcccgtttgt ttttctaccg cgaccgcccg atgattcgaa tctggctcgg actgactttc
     1741 ggcatgctca tgctgatctg gcttcccgcg ctgttttcgt ttgtattcgg gtttgtgctg
     1801 ctttctcagc tattgggact gttcgccgct tgtctgattg gcgcggcctg ctggcttctt
     1861 tcccgtaaaa agtctttgac cgcagctcct tggaaaaaag aactccccgc gctgattacg
     1921 tgcggcgcgc tgtttctgtt ttgtctcgtt cttctttcca gccacaccat tgtcgaacgc
     1981 gacggcgcgc tgtatgttgg acagtcaacg tacggcgacc ttgccatgca cctcggattt
     2041 atgacgagca tcgcggcgca ggagacgttc ccgccaatgt actcgatctg tcccggcacg
     2101 cccgtcgggt atccgttttt gtcggacagc gtgagcgcga ccttcctcaa gctcggcgcg
     2161 tcgctgcgct ttgccgcaat attgcccgcc gcgttcgcgt ttgtgatcgt gatcgtc
//
LOCUS       DAOMGY010000265         2211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_55447_length_2211_cov_3.130334, whole genome shotgun sequence.
ACCESSION   DAOMGY010000265 DAOMGY010000000
VERSION     DAOMGY010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55447_length_2211_cov_3.130334"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLR57_04845"
     CDS             complement(<1..295)
                     /locus_tag="PLR57_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40816.1"
                     /translation="MKRHFHSFGETANAAVALEHLGALILDSLSGEKLERETVAAACD
                     ALSQKLSGEEYLQMLVSSGLSRIQAAEELAFAKNMFSRSYLEERLKHELGGLED"
     gene            complement(292..1413)
                     /locus_tag="PLR57_04850"
     CDS             complement(292..1413)
                     /locus_tag="PLR57_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-protein synthetase"
                     /protein_id="HQQ40817.1"
                     /translation="MSRMAKIFFSRNPYNSKRADNAFLDAVRENVRFQAAHCPVYARI
                     LENCGFSPDELKNEADLYRIPPLPTLHFKRNSLFSVPESEFALVATSSGTKGAKSTVA
                     FDKKTLFYGFGMAARFFLHHHVVSLLPTNYIVLGYEPSKHTSIGAVKTAYGVTKFTPA
                     LSRTYALKDTGKGYEPNQSGVLEALERYAKQDFPVRFVGFPSYLYFLVSELKKSGVRY
                     QLNPHSKILLGGGWKQFAGNEIDRNELFALVRETLGIEADQCLEFFSAVEHPLPYCKC
                     KNGHFHIPVYSRVIIRDAITLEPVADGMPGLLNFVTPLVNSMPLVSVMTDDIAVKRPA
                     EDCGCGNPKPFFDLLGRANVKDVKTCAAGANERLTGGGI"
     gene            complement(1592..1933)
                     /locus_tag="PLR57_04855"
     CDS             complement(1592..1933)
                     /locus_tag="PLR57_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ40818.1"
                     /translation="MAISLQKANIEDCAEIHALQIEAFASLLEKYRDTATNPGAEPLE
                     RIVQRMEQPHTDYYFIRLDGENIGAIRIVRLDDTACRIAPVFLLPTFQNKGFAQQAIS
                     LVEALYPAAAR"
BASE COUNT          512 a          612 c          530 g          557 t
ORIGIN      
        1 cgtcttcaag gccgccgagt tcatgcttca gccgttcctc gagatacgag cgtgaaaaca
       61 tgtttttcgc aaacgcaagt tcttctgccg cttgaatcct gctcaaaccc gacgaaacca
      121 gcatttgtaa atactcttca ccgcttagtt tctgtgaaag cgcgtcgcag gcggctgcaa
      181 ccgtttcccg ttccagtttt tcgccggaaa gcgaatccag aatcagcgcg ccgagatgtt
      241 ccagtgcaac ggcggcattt gcagtttcac caaaagaatg aaaatgacgc ttcatatgcc
      301 gcctcccgtc aaacgttcat tcgcacccgc cgcgcaggtt tttacgtcct tgacgttcgc
      361 cctgcccagc aggtcgaaga aaggtttggg gttcccgcag ccgcagtcct cggcaggccg
      421 cttcaccgca atatcatccg tcatcacgct tacaagcggc atgctgttaa caagcggggt
      481 cacaaagttc aacagccccg gcattccgtc cgcaacaggc tccaacgtga tcgcgtcccg
      541 gatgatcacg cggctgtaaa ccggaatatg gaagtgcccg tttttgcatt tgcagtacgg
      601 cagaggatgt tcaaccgcac tgaaaaactc caagcattga tcagcttcga ttccaagcgt
      661 ttcgcgaacc agcgcaaaca attcgttccg gtctatttcg tttccggcaa actgtttcca
      721 gccgccgcct aatagaatct tggaatgtgg attgagctgg tagcgaacgc cgctcttttt
      781 cagctcgctg accagaaagt acagatagga cgggaacccg acaaaccgca cgggaaagtc
      841 ctgctttgcg taccgctcca gcgcttcgag tacgccgctt tgattcggtt cgtagccttt
      901 gcccgtatct ttgagcgcat aggttcggga cagcgcgggc gtaaatttcg ttaccccgta
      961 agccgtcttg actgcgccta tcgaagtatg cttgcttggt tcgtacccga ggacaatata
     1021 attcgttggc agcagcgaaa cgacatgatg atgcagaaag aatcgcgcag ccatgccgaa
     1081 cccgtagaac agcgtttttt tatcgaatgc aacggtgctc tttgcgcctt ttgtgccgga
     1141 agaagttgca accagagcaa actcgctttc gggaacggag aacagcgaat ttcgtttgaa
     1201 gtgcagcgtc ggcagaggcg gaatccggta caggtcggct tcgttcttga gttcgtcagg
     1261 tgaaaatcca cagttttcca aaatacgcgc atacaccggg caatgcgctg cctgaaagcg
     1321 gacattctcc cgcaccgcat ccagaaacgc gttgtctgcc cgtttggagt tgtatggatt
     1381 tctcgaaaag aagatcttcg ccattctact catcggcata ctccaaaatt cttgtctgaa
     1441 cctttgccgt ctcccgattc ggctaaactc gcttctcgta aaacacaatc gtcattcccg
     1501 gctgaagttc ctcttccttg cctgtctgcc gatatcctaa tttttcatat aaatggcaaa
     1561 gctttgattc ctgcttgatc gtatccggtt ctcagcgcgc cgccgctgga tacaacgctt
     1621 ccaccagact aatcgcctgc tgggcgaatc ccttattctg gaatgtcggc agcaggaaga
     1681 cgggcgcaat ccggcaagcc gtgtcgtcaa gccgcacaat gcggattgcg ccgatgtttt
     1741 ccccatccag ccgaataaaa tagtagtcgg tatgcggctg ttccatgcgc tggacgattc
     1801 gctctaacgg ctccgcgccg gggttggttg cggtgtcgcg gtacttttcc agcaaactcg
     1861 caaacgcttc tatttgtagt gcgtggattt cggcacaatc ttcgatgttt gctttttgaa
     1921 gcgaaattgc catgccgcac tcctttaaat tgtttttgca ttgttactca ataaggtagc
     1981 acaattccag acagaaaaaa agacaaacca acgttggttt gctttttctg taaaaatacg
     2041 acagctgaag atccaagttt ataatcgagc aagatttaac cgacaggcgg aactctcatc
     2101 tgctccagct ttttcgccgc aagaaacgct ttttgcagaa acgcttccgc agatagtgtg
     2161 ccgtattccc agttcatcgc ttcaatcgcc gttgcgccgc gataacccga t
//
LOCUS       DAOMGY010000266         2209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_55509_length_2209_cov_3.502321, whole genome shotgun sequence.
ACCESSION   DAOMGY010000266 DAOMGY010000000
VERSION     DAOMGY010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55509_length_2209_cov_3.502321"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /gene="scfB"
                     /locus_tag="PLR57_04860"
     CDS             <1..1024
                     /gene="scfB"
                     /locus_tag="PLR57_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HQQ40819.1"
                     /translation="ATGDFHGARMLMPLDVGKRALDFLMEHSGTRKHLEVDLFGGEPL
                     MNWPVCVGLVEYGRELEKKFGKEIHFTITTNCVALDDDKIDFINREMHNVVISLDGRK
                     ETHDALRPTVNGKGSFDIILKNAQKLVAGRGDKEYYIRGTFTRNNLDFLEDVKALTGF
                     GFDQISIEPVVLSPESPYSLLPEHMERIDREYDSLAAHYIEARRNPETWFNFFHFMFD
                     PEGGPCLRKRITGCGAGTEYVAVTPDGELYPCHQFVGMQEWKLGTLETGILRDDLQKS
                     FLGCNVCTKETCQNCWAKYYCSGGCMANAVLYGGALNQPHEISCHLMRKRFECAIGVY
                     AAEQNE"
     gene            1017..>2209
                     /locus_tag="PLR57_04865"
     CDS             1017..>2209
                     /locus_tag="PLR57_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HQQ40820.1"
                     /translation="MNNRMIPRAPEVCSEALVENIIESERVSAMTARALIRRGVSSAE
                     EARRFLHPSELSLHDPYLLPDMERAVERIRLAIERSERICVYGDYDADGVCSTAMLTG
                     RLQSMGALVEYTIPLRHSEGYGLSEQSVRKLKERNIGLIITVDNGVSAFDEIALCSAL
                     GMDVIVTDHHSVGKTVPDCVAVVAASRKDSTYPNRYLCGAGVALKLISALNDGAYSDA
                     ELAMAAVATIADVVPLNEENRAIAANGLKYIESVPGFSALLEAAGWKQPRVDEQTVSF
                     VIAPRLNAAGRMSTAMIGVELLLGTEETRLKEIAELLNTDNLRRKEAETEILEEAKRQ
                     IEQEGVIGHVLILKHEAWNPGVIGIVASRLCETYHFPVILFADQNGVLTGSGRSIDGV
                     DLFEN"
BASE COUNT          485 a          610 c          679 g          435 t
ORIGIN      
        1 cgccacgggg gacttccacg gggcgcgtat gctcatgccg ctggacgttg gcaaacgcgc
       61 actcgatttt ttgatggagc acagcggaac cagaaagcat ttggaagtcg atctctttgg
      121 cggagaaccg ctcatgaact ggccggtctg cgtgggcctg gtcgaatacg ggcgggaact
      181 ggagaagaag ttcggtaaag agattcattt tacgatcacg accaactgcg tggcgctgga
      241 cgacgataag atcgacttta tcaaccgcga aatgcacaac gtcgtcatca gtctagacgg
      301 gcgaaaggaa acgcacgacg cgctccgtcc taccgtcaac ggcaaaggct cgtttgatat
      361 catcctgaaa aacgcgcaaa agctcgtcgc cggtcgcggg gacaaggaat actatattcg
      421 cgggacgttt acccgcaata atctggattt tctggaggac gtcaaggcgc tgaccggatt
      481 cggtttcgac cagatttcca tagagccggt cgttctctct ccggaaagcc cgtacagcct
      541 cttgccggag catatggagc ggatcgatcg cgagtacgat tctctcgcgg cgcattatat
      601 cgaagcgcgc agaaacccgg aaacatggtt taactttttc cacttcatgt tcgatcccga
      661 aggcggcccc tgcctgcgca aacgcatcac gggctgcggc gcgggtacgg aatacgtcgc
      721 cgttacgccg gacggggagc tatatccctg ccatcagttc gttggcatgc aggagtggaa
      781 gctcggcacg ctggaaacgg gaatcctgcg ggacgatctg caaaagtcgt ttcttggctg
      841 caacgtctgc acaaaggaaa cctgccagaa ttgctgggca aagtactatt gtagcggcgg
      901 gtgcatggca aacgccgttc tctatggcgg cgcgctgaac cagccgcatg aaatcagctg
      961 ccacctcatg agaaagcgct ttgagtgcgc aatcggcgtt tacgccgccg agcaaaatga
     1021 ataaccgcat gatcccgcgt gcgccggaag tctgttctga ggcgctcgtg gaaaacatca
     1081 tagagagcga gcgcgtttcc gcaatgaccg cgcgggcgct cattcggcgc ggcgtttcct
     1141 cggctgagga agcgcggcgc tttctgcatc ccagcgaact gtcgctgcac gatccgtacc
     1201 tgctgccgga tatggagcgg gcggtcgagc gcatacggct ggcgattgag cgaagcgagc
     1261 gcatctgcgt ttacggcgat tacgacgcgg acggcgtctg ctccacggcg atgttgacgg
     1321 gtcggctcca aagcatgggc gcgttggtcg aatacacaat tccgctcagg cacagcgaag
     1381 ggtacgggct ttccgaacaa tctgtacgca agctcaaaga gcggaatatc ggcctgatta
     1441 tcacggtgga taacggcgtt tccgcgttcg acgagattgc gctgtgttca gcgctcggta
     1501 tggacgtgat cgttaccgac catcacagcg tgggcaaaac cgtgccggac tgcgtggcgg
     1561 tggtcgccgc aagcaggaaa gattccacct atccaaaccg ctatctctgc ggcgcgggag
     1621 ttgcgctcaa actcatcagc gcgctcaacg acggcgcgta ttcggacgcg gagctcgcaa
     1681 tggcagcggt tgctaccatt gcggacgtcg ttccgctcaa cgaagaaaac cgcgcgattg
     1741 ccgctaacgg cttaaagtat attgaaagcg tccccggctt ttccgcgctg ctggaagcgg
     1801 cggggtggaa gcagccgcgc gtggacgagc agacggtttc gtttgtgatt gcaccgcgtc
     1861 tgaacgccgc gggacgcatg tccacggcga tgatcggcgt ggaactgctg ctgggcacgg
     1921 aagaaacgcg cctcaaggag attgccgaac tcctcaacac ggataacctc cgccgcaagg
     1981 aagcggagac ggagattctg gaggaagcca aacgccagat cgagcaggaa ggcgtcatcg
     2041 ggcacgttct gattctcaag cacgaggctt ggaatcccgg cgtgatcggc attgtggctt
     2101 cccggctctg cgaaacatat catttccccg tgatcctctt tgccgatcag aacggcgtgc
     2161 tgacgggttc cggcaggagc atcgacggcg tggacctgtt tgaaaactt
//
LOCUS       DAOMGY010000267         2206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_55626_length_2206_cov_3.337517, whole genome shotgun sequence.
ACCESSION   DAOMGY010000267 DAOMGY010000000
VERSION     DAOMGY010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55626_length_2206_cov_3.337517"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLR57_04870"
     CDS             <1..257
                     /locus_tag="PLR57_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40821.1"
                     /translation="GYFCGTGMHGGSIYVGGEIKREKFPRQVRICEANADDLAKIEPY
                     MEKYVTLFNKQIPTTARRSGCLPRIPTTPTVSFTLPTDPR"
     gene            404..1618
                     /locus_tag="PLR57_04875"
     CDS             404..1618
                     /locus_tag="PLR57_04875"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HQQ40822.1"
                     /translation="MYTAQEIVEYAAAEDVKFIRLAFCDVFGLPKNISIMPEELPRAF
                     SDGISIDASAIRGFGDEAHSDLLLFPDPETLTALPWRPSHGRVVRLLAEIRRSDGTVF
                     SLDTRNLLKAAIAEAAKEGVVVNFGTEFEFYLFRLDADGNATKIPYDSAHYMDIAPLD
                     RGENVRREICLTLCEMGIRPESSHHEDGPGQNEIDFRYSDALSAADNAFTFPSVVRSV
                     AMRNGLAADFSPKPLPDESGNGMHINLSLAKPHTETARDSFMAGLMEHITEITAFLNP
                     LPASYARFGEHKAPRYVTWSPENRSQLIRIPAAKGEFERLELRSPDPACNPYLAFTLI
                     LCAGLDGVRRGLVPPPATNVNLFTADASITKALKTLPKTREEAFALASGSAFVRSVLP
                     EGVFHAYETYNG"
     gene            1636..>2206
                     /locus_tag="PLR57_04880"
     CDS             1636..>2206
                     /locus_tag="PLR57_04880"
                     /inference="COORDINATES: protein motif:HMM:NF015799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HQQ40823.1"
                     /translation="MASGFEKQNSVLIVSASERARESIAGVLLPSRYSPIAMRGSAAE
                     ARRLVLDTPYSIVFINAPLPDEFGGQLALDLAASKLCCVALIVASESYEEVTDKVEDA
                     GVITLSRPCSAQNIRQAAALMSATRSKLADMEKKTATLEEKMEEIRLVNRAKWMLIER
                     RGMDEATAHRYIEKLAMDARQTRRLVAQTL"
BASE COUNT          465 a          700 c          631 g          410 t
ORIGIN      
        1 tggggtattt ctgcggcacg ggcatgcacg gcggatcgat ctacgtcggc ggtgaaatca
       61 agcgcgaaaa gttcccgcgc caggtgcgca tttgcgaagc gaacgccgac gacctggcca
      121 aaattgaacc gtatatggaa aaatatgtta cactgtttaa taagcagatt ccaacgacgg
      181 cacgccgttc tggctgctta ccccgaattc caacgacccc taccgtcagc tttacgttac
      241 caactgatcc tcgctgattc aaatgcggca aagtacaatc ggaaccggcc ggttttgacg
      301 aagcaatcga aacaggcggt tccggttttg tatttcaggc aaacggagaa aaacacacga
      361 ccgttcaaca gctcagtata tacaggaaag ggagccaaga aagatgtata ccgctcagga
      421 aatcgtagaa tacgccgccg cggaggacgt caagttcatc cgcctcgctt tttgcgacgt
      481 cttcgggctg ccgaagaata tctcgatcat gccggaggag ctgccgcgcg cgttttcgga
      541 cggcatctcc atcgacgcct cggccatccg cgggttcgga gacgaagcgc attccgacct
      601 gctgctcttc cccgacccgg aaacgctcac ggcgctgccc tggcggccca gccacgggcg
      661 cgtggtaagg ctgcttgcgg aaatccgccg ctcggacggc accgtgtttt cgctggacac
      721 gcggaacctt ttaaaagccg cgattgccga agccgcgaag gaaggcgttg tggtcaactt
      781 tggcacggag tttgagtttt acctcttccg cctcgacgcg gacggcaacg cgaccaagat
      841 tccctacgac agcgcgcact atatggatat cgcacctctc gaccgcggcg agaacgtccg
      901 cagggagatc tgcctgacgc tctgcgaaat gggcatccgt cccgagagct cgcatcacga
      961 ggacggcccc ggccagaacg aaatcgattt tcgctacagc gacgcgctga gcgccgccga
     1021 caacgccttc accttccctt cggttgttcg ctccgtcgcc atgcgcaacg gccttgccgc
     1081 cgacttttcg ccaaagccgc tgcccgacga gagcggcaac ggcatgcata tcaacctttc
     1141 acttgccaag ccgcatacgg aaaccgcgcg ggattcgttt atggcgggac tgatggagca
     1201 tattaccgag attacggcgt ttctcaatcc gctgcccgct tcctatgccc gctttggcga
     1261 gcataaagca ccgcggtacg tcacctggtc accggaaaac cgctcacagc tcatccgcat
     1321 ccccgccgca aaaggcgagt tcgaacggct cgaactgcgt tcgcccgacc ccgcctgcaa
     1381 tccctacctt gcgtttacac tgattctttg cgcggggctg gacggcgtgc gccgtggtct
     1441 cgttccgccg ccggcaacca acgtaaacct ctttacggcg gacgcttcca ttacaaaagc
     1501 gctgaaaacg ctgcccaaga cccgtgagga ggcgttcgcg ctcgcatccg ggagcgcgtt
     1561 cgtccgctcg gttctgccgg aaggcgtgtt ccatgcttac gaaacctaca acggctgaag
     1621 agcggagggc aaacgttggc gtccgggttt gaaaagcaga atagtgtatt gatcgtttcg
     1681 gcgtccgaac gcgcgcggga gagcatcgcg ggcgttctgt tgccgtcccg ctattcgccc
     1741 atcgccatgc gcggctccgc cgccgaagcg cgccggcttg tgctcgacac gccctactcg
     1801 atcgttttca tcaacgcgcc gctgccggac gagttcggcg gccagctcgc gctcgacctc
     1861 gccgcgagca aactctgctg cgtggcgctg atcgttgcca gcgaatcgta tgaagaagtc
     1921 acggacaaag tggaagacgc gggcgtgatt acgctgtccc gcccctgctc ggcgcagaac
     1981 attcgccagg ccgcggcgct gatgtccgcc acgcgctcga agcttgcgga catggagaaa
     2041 aagaccgcca cgctcgaaga gaagatggag gaaatccgcc tcgtcaaccg cgccaagtgg
     2101 atgctcatcg aacggcgcgg catggacgaa gccaccgcgc accgctatat cgaaaaactc
     2161 gccatggacg cgcgccagac ccgcaggctc gtggcgcaga cgctga
//
LOCUS       DAOMGY010000268         2204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_55693_length_2204_cov_3.542578, whole genome shotgun sequence.
ACCESSION   DAOMGY010000268 DAOMGY010000000
VERSION     DAOMGY010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55693_length_2204_cov_3.542578"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLR57_04885"
     CDS             <1..1035
                     /locus_tag="PLR57_04885"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQQ40824.1"
                     /translation="IDTILARFMVIPVFLETIGVKQKKFKVTDKSSSKPQASNLVYII
                     PQLVMLGLSVAGLVRYLSGKYGMALFYSSFIAFWLIYNIINLLYAVYFMMGRKAYRTS
                     ERFDADIPVRLTYNKQVVHAVTRNISEGGFLFTLACPLYLPNDEELKFTLRSDRYRAR
                     VAGKIAYVNSEKDQWLYHVKLTGELTDKQRRQYLQLVYDRAPSLPKSMNVWVTAFDDI
                     VNNATARLEKQQAEMRALPRIELDRKAVFEEGGAATLVNFNYKYFLVRDLTADAPVLT
                     IALNRGVRIVLEATDLPVYRAGEKLMRVVNWQELAGSKAFREVLDDWIFEYVQRYTGQ
                     NHQGKLLPQN"
     gene            1270..>2204
                     /locus_tag="PLR57_04890"
     CDS             1270..>2204
                     /locus_tag="PLR57_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40825.1"
                     /translation="MQIVLTILRLIAFALSTAGYVAFVRAYWRISPRASYVFVLSALA
                     CLMYFAGLLNVLLIGAYALFFGGVALFILVLARRKMALAYNRSSLSAMNIGFALGLAF
                     LIASLIDTKFVHYDNFSHWAVVVKSMLIENQIPTAASAIIDFKSYPLGSSSFLYYFCR
                     VVGNSEGVMLIGQGLLLTACFYAVFGVIRDQKRFLLSALLGLGCAALAFFNISIRVNN
                     LLVDFLLPALALAALGSLSVEQASFRSRCLSVVPVLGLLVITKNTGIFFAALVYVFLL
                     YRAVKLRNELEKPRPYVWLALAAIAASLLPLVLWN"
BASE COUNT          444 a          615 c          640 g          505 t
ORIGIN      
        1 atcgatacga tccttgcgcg gtttatggtg attccggtct ttctggagac catcggcgtc
       61 aagcagaaga agttcaaggt gacggacaag agctccagca aaccgcaggc ttcgaacctt
      121 gtttacatca ttccgcagct ggtcatgctt ggcttgtccg ttgcggggct ggttcgctac
      181 ttgagcggca aatacggaat ggcgctgttc tacagctcgt tcatcgcatt ctggctgatc
      241 tacaacatca tcaatctcct gtacgcggtg tacttcatga tgggcagaaa ggcctaccgc
      301 acgagcgaac ggtttgacgc ggatatcccg gtcaggctga cgtataataa gcaggtcgtc
      361 catgccgtaa cgagaaacat ctcggaaggc ggattcctct ttacgctcgc ctgcccactg
      421 tacctgccga acgacgaaga actcaagttt actctgcgga gcgaccgcta ccgcgcgcga
      481 gtggcgggaa agatcgccta tgtcaacagc gaaaaggacc agtggctcta ccatgtaaag
      541 ctcaccggag agttgaccga caagcagaga cggcagtacc tgcagctggt ttacgaccgc
      601 gcgccgtcgc tgccgaaatc catgaacgtc tgggttaccg cgttcgacga tatcgtcaac
      661 aacgccacgg cccggctgga gaagcagcag gctgaaatgc gcgccctgcc gaggattgag
      721 cttgacagga aggcggtgtt tgaagagggc ggagcggcaa cgctcgtcaa cttcaactac
      781 aagtactttc tcgttcgcga tctgacggcg gatgctccgg ttttgacaat cgctctgaac
      841 agaggcgtac gaatcgttct ggaagcgacg gatctgccgg tataccgcgc gggcgaaaag
      901 ctcatgcgcg ttgtgaactg gcaggagctt gccggcagca aagcatttcg ggaagtgctc
      961 gacgactgga tcttcgaata cgtacaacga tacacgggac agaaccatca gggcaaactg
     1021 cttccccaga actgaacggg ttccaaacag gccgcgccga acgcgcggcc tgtttttgca
     1081 gatgcgccag ccgagcgatg gatgcggcgc agacggagat gccggatacg cgtgacaagc
     1141 cttttttccg gcggaaagac agtgtatact ggttcatata gacggcttcg aataccaaac
     1201 ggcaaacaat ccccgcggga cagatgcggg tgaaacttcc cggctacttc ggaaaggaaa
     1261 gagattgtta tgcagatcgt cttaaccatt ctcaggctga tcgcgtttgc gctttccacg
     1321 gcgggctatg tcgcgtttgt ccgtgcgtac tggcgaatct ctccgcgcgc aagctacgtc
     1381 ttcgttctct ccgcgcttgc ctgcctgatg tactttgcgg gattgctgaa cgtactcctc
     1441 ataggcgcgt acgcgctgtt cttcggcggc gtcgcgcttt ttattcttgt gctggcgagg
     1501 cgcaaaatgg cgcttgcata caacaggtcc tcgcttagcg caatgaacat cggctttgcg
     1561 ctgggacttg cgtttctgat cgcttctctg atcgacacga agttcgtcca ttacgataac
     1621 ttttcgcact gggcggtggt cgtcaagagc atgctgatcg agaaccaaat cccgaccgcc
     1681 gcgagcgcga tcatcgactt taagtcctat ccgctgggca gctcttcgtt tctctattac
     1741 ttctgccgcg tggtgggcaa tagcgaaggc gtcatgctga tcgggcaggg attgctgctc
     1801 acagcgtgct tctacgcggt gttcggcgtc attcgcgacc agaagcgctt tctgctctcc
     1861 gcgctgcttg gacttgggtg cgcggcactg gcgtttttca acatcagcat tcgggtcaac
     1921 aacctgctcg tggactttct gctgcccgct ctcgcgctgg cggcgcttgg cagcctgagc
     1981 gtggagcagg cgagtttccg cagccggtgc ctttccgtcg tgcccgtgct ggggctgctt
     2041 gtcatcacga aaaacacggg catcttcttt gccgcgctgg tgtacgtgtt tctgctctac
     2101 cgcgcggtca aactgcgaaa tgagctggaa aaaccgcgtc cctacgtttg gctggcgctg
     2161 gcggcaatcg ccgcgtcgct gctgccgctg gtgctttgga actt
//
LOCUS       DAOMGY010000269         2204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_55701_length_2204_cov_3.147510, whole genome shotgun sequence.
ACCESSION   DAOMGY010000269 DAOMGY010000000
VERSION     DAOMGY010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55701_length_2204_cov_3.147510"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="PLR57_04895"
     CDS             complement(<1..145)
                     /locus_tag="PLR57_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HQQ40826.1"
                     /translation="MERTLITRIIPGTEVKVQGFVERVRNTGAMAFLVLRDRTGKVQV
                     TIEK"
     gene            complement(521..1687)
                     /locus_tag="PLR57_04900"
     CDS             complement(521..1687)
                     /locus_tag="PLR57_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQQ40827.1"
                     /translation="MKELNRTAERFTDSVIRRMTRISYQYGAINLAQGFPDFDPPTEI
                     TDRLAEISGVGPHQYAITMGAPNFRQALAKKCGRCMGRTLDADTEMVVTIGSTEAMVD
                     TLFAITNPGDKIILFSPYFENYTAQILMVGCEAVFVPLVPPAFGFDPNALEDAFKQNV
                     KAILICNPSNPSGKVFSPEELKIIAEMAVRYDTYVIMDEVYEHIIYAPYKHTYMNSLP
                     GMWERTISCSSLSKTYSITGWRLGYAIAPKLIMDKIKQYHDFNAVGAAAILMEAAIVG
                     LEMPESYYDGLAAHYAHMKELFTGGLKSIGIPFTDPQGAYFVLADIAPFLKPGQTDVA
                     FCEEMAEKVGVACVPGTSFFKEKVNNIVRLHFAKKDETLVAALDRLSDIKAKLS"
     gene            complement(1742..2005)
                     /locus_tag="PLR57_04905"
     CDS             complement(1742..2005)
                     /locus_tag="PLR57_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40828.1"
                     /translation="MQDPSVHFLHAGIFHQVLVKVKVVKVAEDEPAGVADAAISLYQP
                     LEDLVGDADIDGIVFRISDEPKLIKLLSAHGISAVTQEELGIR"
     gene            2018..>2204
                     /locus_tag="PLR57_04910"
     CDS             2018..>2204
                     /locus_tag="PLR57_04910"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQQ40829.1"
                     /translation="MIQRVRKAYDDYQFHVVYYTIYNFCTVDLSAVYLDVSKDRLYTS
                     KAGSNARKSAQSAMYIIL"
BASE COUNT          461 a          635 c          590 g          518 t
ORIGIN      
        1 ctttttcaat cgtaacctgc accttgcccg tgcggtcgcg cagcaccaga aacgccatgg
       61 cacccgtatt tcgcacgcgt tccacaaacc cctgcacctt gacctcggtt ccggggatga
      121 ttctggtgat caatgttcgt tccatattcc attcttcctt tcttcattag agttcagttg
      181 aacgcggaac aaacgcatca tatccggcat catccgtatg ccgcattcca tcgccccctc
      241 tcgcttatcg ctgatcagtc atagaaaaaa cccgtccgga caaactatgc ccgaacgggt
      301 aggaaaccag aggaacgttt ccccaaaagg cgaaacgcgg ttggatttcc tcgcgggcac
      361 agctcgtttc aagcacttgc gcctgcgggg tacttccgcg gctcaagtgc tatcgatcca
      421 tgtccttttt gaggcagatc cgaagcacgg ttggctctcc ttgtaggtga aattttctac
      481 agtatagtct tgcgcgcctt ttgtgtcaag cgggggctcg ctagctcagc tttgccttga
      541 tgtcgctcag gcggtcgagc gcggcaacga gcgtttcgtc cttctttgca aagtgcagac
      601 gcacgatgtt gttgaccttt tccttaaaga acgacgtgcc gggtacgcag gcaacgccaa
      661 ccttctctgc catctcctcg cagaacgcaa cgtcggtttg tcccggcttg aggaacggcg
      721 cgatatccgc cagcacgaag tacgcaccct gcgggtcggt aaacggaatg ccgattgact
      781 tcaacccgcc cgtaaagagc tccttcatgt gcgcatagtg cgcggccagc ccgtcgtaat
      841 agctctccgg catctccaag cccacgatag ccgcttccat gaggatggcc gccgcgccta
      901 cggcgttgaa gtcgtggtac tgcttgattt tatccataat caatttcggg gcgatggcat
      961 agccaagccg ccagcccgtg atggagtagg tcttggaaag cgaactgcag gagatcgtcc
     1021 gttcccacat gccgggcagg ctgttcatat acgtgtgctt gtacggcgcg tagatgatgt
     1081 gctcgtagac ttcgtccata atcacatacg tgtcgtagcg caccgccatt tcggcaatga
     1141 ttttgagctc ctccggcgaa aaaacctttc cgcttgggtt ggaggggttg cagattaaaa
     1201 tcgccttgac gttctgctta aacgcatcct ccagcgcgtt ggggtcgaac ccgaacgcag
     1261 gcggaacgag cggcacgaac accgcctcgc agccgaccat cagaatctgc gcggtatagt
     1321 tttcgaaata cggcgaaaag aggatgatct tgtcgccggg gttcgtaatg gcaaacagcg
     1381 tatccaccat ggcttccgtc gagccgatgg taaccaccat ttcggtgtcc gcatcgagcg
     1441 tgcgtcccat gcagcggccg cactttttgg ccagcgcctg ccggaaattc ggcgcgccca
     1501 tcgtgatggc gtactggtgc gggccaacgc cgctgatctc cgcgaggcgg tcggtaatct
     1561 ccgttggcgg gtcaaagtcc ggaaatccct gggccaggtt gattgcgccg tattggtagg
     1621 aaatccgcgt catgcgccgg attacggaat ccgtaaaccg ctctgcggtt cgattgagct
     1681 ctttcatgtg ttatccctcg ttgtttcgaa aaattcgtct cgcgtccgtt cgacaggttg
     1741 cctagcggat gcccagttct tcctgcgtca ccgccgaaat gccgtgtgcg ctcagcagtt
     1801 taataagctt tggctcgtcg gagatgcgga agactatacc gtcgatatcc gcatctccga
     1861 cgagatcctc cagaggctgg tagaggctta tcgccgcatc cgcaacacca gcaggttcat
     1921 cctcggcaac ctttacgact ttaactttga caaggacctg gtgaaatatg ccggcatgga
     1981 ggaaatggac agatggatcc tgcacaggct ccaggaagtg atccagcggg tccggaaggc
     2041 ctatgacgat taccagttcc acgtcgtgta ttacacgatt tacaatttct gcacggtcga
     2101 cctgagcgcc gtataccttg atgtgtcgaa ggacaggctc tacacttcca aagccggctc
     2161 caatgccagg aaatccgcgc agagcgcgat gtatattatc ctgg
//
LOCUS       DAOMGY010000270         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_55972_length_2197_cov_5.490663, whole genome shotgun sequence.
ACCESSION   DAOMGY010000270 DAOMGY010000000
VERSION     DAOMGY010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55972_length_2197_cov_5.490663"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PLR57_04915"
     CDS             <1..681
                     /locus_tag="PLR57_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HQQ40830.1"
                     /translation="GSAEPLFVEGGLFTRDQLTAMTEAQSYGDLPQAMADALNELEKK
                     LKIQAEPQLVSVMVDYGYLKNCLLTSADAKDPFVKQYFTALCDFDNVITFFRMRAMGA
                     QKEDLKEMLLPSAGVKAQSLIEAYELSAETLTRALSGSVAKSALQEGLNRMLVSGNIS
                     TLEKARDDYLLSLVNGHRHEVMTIFPIVGYLLARDRESKAVRLILTVKRNGLDDGVIT
                     ERLRELYG"
     gene            674..991
                     /locus_tag="PLR57_04920"
     CDS             674..991
                     /locus_tag="PLR57_04920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HQQ40831.1"
                     /translation="MAKIGVIGDKDSILLFQAVGLDVFFEDDGERANRTLHRLAREGY
                     AAVFVTEKLYPACAETIQEYQASAYPAIIPIPDNQGSRGVGAEALRKNVEKAVGMDIL
                     ANS"
     gene            1080..>2197
                     /locus_tag="PLR57_04925"
     CDS             1080..>2197
                     /locus_tag="PLR57_04925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HQQ40832.1"
                     /translation="MQGTIIKVSGPLIVAGGMADVQMYDVVRVSEKHLIGEVIELRGD
                     KASIQVYEETGGIGPGEPVETTGAPLSVELAPGLIESIYDGIQRPLNVIRERAGDRIT
                     RGIMVDALDHEKLWKFAPTAKVGDQLTGGDILGVVQETEAVEHRVMVPPNVSGKLTWI
                     FEGEANIVQPVAKLEKSDGSVVELPMLQKWPVRKGRPYTEKVSPKEPMVTGQRVIDTL
                     FPVAKGGTAAVPGPFGSGKTVVQHQLAKWADADIIVYVGCGERGNEMTDVLMEFPELK
                     DPRTGLSLMKRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYKVAIMADSTSRWA
                     EALREMSGRLEEMPGEEGYPAYLSSRLSEFYERAGIVT"
BASE COUNT          500 a          591 c          680 g          426 t
ORIGIN      
        1 ggttcagccg aaccgctttt cgtagaaggc ggactgttca cgcgcgatca gctcaccgca
       61 atgacggaag cgcagtcata cggagatctg ccgcaagcaa tggcggacgc gctgaacgag
      121 ctggaaaaga aactcaaaat tcaggcggaa ccgcagttgg tcagcgtgat ggtggattac
      181 ggctacctca aaaactgcct gctgacttcc gccgatgcaa aagacccgtt tgtaaagcag
      241 tattttacag cgctctgcga ttttgacaat gtgattacct tcttccgcat gcgcgctatg
      301 ggtgctcaga aggaggattt aaaagaaatg ctcctgccgt cggcaggcgt gaaggcacag
      361 tcgctgattg aagcctacga gctttccgcc gagacgctga ccagagcgct ttccggcagc
      421 gttgcaaaaa gcgcgctgca ggaggggcta aaccggatgc tcgtctccgg caacatctct
      481 acgcttgaaa aagcgcggga tgattatctg ctctcgctcg tcaacggcca tcgccacgag
      541 gtgatgacga tcttcccgat cgtcggctac ctgcttgcgc gggaccgcga gtcaaaggcg
      601 gtgcgcctga tcttaaccgt caagcgcaac gggctcgacg acggcgtgat cacggaaagg
      661 ctgagggaac tgtatggcta aaattggcgt tattggcgac aaggattcca tcctgctgtt
      721 tcaggcggta ggcttggacg tgttttttga ggacgacggt gagcgcgcca accgtacgct
      781 ccaccgcctt gcgcgggaag ggtacgccgc cgtgttcgtt accgaaaagc tgtatcctgc
      841 gtgcgcggaa acgattcagg agtatcaggc cagtgcttat cccgcgatca ttccgattcc
      901 ggataatcag ggctcgcgcg gggtcggcgc ggaagcgctc aggaaaaatg tggaaaaagc
      961 cgtcggcatg gacatcctcg cgaattcata gcaggacgga cttaccggct taaactcgaa
     1021 aaatatgcgg attcggtaga aaccttccgc gacagatagt atacgaaagg gaaaccaaca
     1081 tgcaaggaac gatcatcaag gtctcggggc cgctcatcgt tgcaggcggc atggccgacg
     1141 tgcagatgta cgacgttgtg cgcgtatccg aaaagcattt gatcggagaa gtcattgagc
     1201 tgcgcggcga caaggcctcc attcaagtct acgaggaaac gggcggaatc gggcccggcg
     1261 agccggtgga aaccacgggc gcgccgcttt cggttgaact cgctccgggg ctgatcgagt
     1321 cgatttacga cggcatccag cgtccgctca acgtcattcg cgagcgcgcg ggagaccgta
     1381 ttacgcgcgg catcatggtg gacgcgctgg atcacgaaaa attatggaag tttgctccta
     1441 ccgcgaaggt gggggatcaa ctcaccggcg gcgatatctt aggcgtcgtt caggaaacgg
     1501 aagcggtgga acaccgcgtt atggttccgc cgaacgtctc cggcaagctc acctggatct
     1561 tcgaaggtga ggcgaacatc gttcagcccg tcgcgaagct ggaaaagagt gacggatcgg
     1621 ttgtcgaact gccgatgctc cagaagtggc cggttcgtaa aggccgtccc tacaccgaaa
     1681 aggtttcgcc aaaagagcct atggtcaccg gccagcgcgt tatcgacacg ctgttccccg
     1741 tggcaaaagg cggcacggcg gcggttcccg gcccgttcgg cagcggcaag acggttgttc
     1801 agcaccagct cgcaaagtgg gcggatgcgg acatcatcgt ctacgttggc tgcggtgagc
     1861 gcggcaacga gatgacggac gttctgatgg agttcccgga actgaaagac ccgcgtacgg
     1921 gactttcgct gatgaagcga acggtgctga ttgccaacac ctccgacatg ccggtggctg
     1981 cgcgtgaagc ttccatttac acgggcatta cgattgcgga gtacttccgc gacatgggct
     2041 ataaagtcgc catcatggcg gactcgacct cccgctgggc ggaagcgctc cgcgaaatga
     2101 gcggacgact cgaagaaatg ccgggcgaag aaggttaccc tgcgtacctt tccagccgtt
     2161 tgagcgagtt ttacgaaagg gcgggcatcg tcacctg
//
LOCUS       DAOMGY010000271         2189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_56282_length_2189_cov_3.170572, whole genome shotgun sequence.
ACCESSION   DAOMGY010000271 DAOMGY010000000
VERSION     DAOMGY010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56282_length_2189_cov_3.170572"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1488
                     /locus_tag="PLR57_04930"
     CDS             124..1488
                     /locus_tag="PLR57_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQQ40833.1"
                     /translation="METQLKKKYGLLTAISMVVGIVIGSGVFFKAEKILTKTGGDLPL
                     GILAWVIGGVIMMICSYVFAVMATKYEYVNGVVDYSEVTVGKGYGYMMGWFFATIYYP
                     TLTSVLAWVSARYTAVLFGWSIVGPECMVIAAFYLIASYAVNALSPKLAGKFQVSTTI
                     IKLVPLLLMGVVGCIVGFTNGMTVQNFTNVVTEVASKSGALLTAVCATAFAYEGWVIA
                     TSINAELRDSKKNLPIALVLGALIVIVVYILYYIGLAGVVTNETLMAGGQEGAKQAFT
                     NLFSNVGGTLLFVFVIISCLGTLNGLMMGCTRGFYSLAARKVGPSPATYIEISPKTNM
                     ATNSSALGLLLSAAWLAYFFGANLVEKSWFGVLSFDSSELPIITVYAMYIPMFIAFMV
                     KQKDTNWFNRFFMPILGVAGCLFMVYAAIVSHQMAVVWYLVIFAAIMLVGVFFGKKKD
                     IEVK"
     gene            complement(1550..>2189)
                     /locus_tag="PLR57_04935"
     CDS             complement(1550..>2189)
                     /locus_tag="PLR57_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HQQ40834.1"
                     /translation="PDSNPSVSEYLSLLAETRIAGDEISIERCNARKSVLSGSAEAYT
                     AILESDAILIIFPLYFFCLPGLLTRFLQDYAAFVKSQERTGKVPRVYAMVNCGFPEDE
                     INGEALRVVESFCRAIGAHWRFGLMIGGGGMLLGAKDAPFMKGVMGTIDEYLSLIKND
                     ILTQKDSELQTMRAFVKFPSKLYFMMGNLGWKMQAKSNGLKTRDLRRKPYQK"
BASE COUNT          479 a          577 c          590 g          543 t
ORIGIN      
        1 agagaggctc tgaaatcgct ttctttttac aattgaaaac gctaaacaac caaaatccat
       61 ttggttgaca aaatatatat gatattgtta aataagaaca ctgacggaaa gggagtttgg
      121 ggtatggaaa cacagttaaa aaagaagtat ggccttctca ccgcaatttc gatggtcgtg
      181 ggcatcgtca tcggaagcgg cgttttcttt aaggccgaaa agattctaac caagaccggc
      241 ggagacctgc cgctgggcat cctcgcgtgg gttatcggcg gcgttatcat gatgatctgc
      301 tcgtatgtgt tcgccgtgat ggcaacaaaa tacgaatacg tcaacggagt agtggactat
      361 tcggaagtaa ccgtcggcaa gggatacggc tacatgatgg gctggttctt cgccacgatt
      421 tattacccga cgctgaccag cgtgctcgcg tgggtttccg cgcgctacac ggcggttctg
      481 ttcggctggt ccatcgtagg gccggagtgc atggtgattg cggcattcta tctgatcgcg
      541 agctacgccg tcaacgcgct ttcgccgaag cttgcgggca aattccaggt cagcacgacg
      601 ataatcaagc ttgttccgct gcttttgatg ggcgttgtcg gctgtatcgt cggctttacc
      661 aacggcatga ccgtgcaaaa ctttacgaat gtcgtaacgg aagtcgcttc caagagcggc
      721 gcgctgctca cggcagtctg cgccacggcg tttgcctatg aaggctgggt catcgcaacg
      781 agcatcaacg cggaactgcg ggattcgaaa aagaatcttc ccatcgcgct tgttctcggc
      841 gcgctgatcg tcatcgttgt ttatatcctg tactacatcg gccttgcggg cgttgttacc
      901 aacgaaaccc ttatggcggg cggtcaggaa ggcgcaaagc aggcgtttac gaatctcttc
      961 tcgaacgtcg gcggaacgct gttgttcgtg ttcgtcatca tctcctgcct tggcacgctc
     1021 aacggcctca tgatgggctg cacgcgcggc ttctactcgc tggcggcgcg caaggtcggc
     1081 ccgagccccg caacgtatat tgagatcagc ccgaagacca acatggcgac gaactcttcc
     1141 gcgcttggtc tgctgctctc cgcggcatgg cttgcgtact tctttggcgc gaacctcgtt
     1201 gaaaaatcat ggttcggcgt gctctcgttc gactcttccg aactgccgat catcacggtg
     1261 tacgccatgt acattccgat gttcatcgcg ttcatggtca agcagaagga tacgaactgg
     1321 ttcaaccgct tcttcatgcc gatcctcggt gtagcgggct gcctgttcat ggtatacgcg
     1381 gcaatcgttt ctcatcagat ggctgtcgtg tggtatctcg tgatcttcgc ggcgatcatg
     1441 ctcgttggcg tcttcttcgg aaagaagaaa gatatcgaag tcaaatagca atccactcag
     1501 ttaacaaaca gcctgccgga aacggcaggc tgttttgtgt ttcaatgcgt cacttttgat
     1561 atggctttct gcgcagatcg cgcgtcttta gaccgttgct cttggcctgc atcttccagc
     1621 cgagattgcc catcataaaa tagagcttgc tcggaaattt cacaaaggcg cgcatggtct
     1681 gaagttcgga atctttctga gtaaggatat cgtttttaat aagcgaaaga tactcgtcga
     1741 ttgtgcccat aacgcccttc ataaacggcg cgtcctttgc gccaagcagc attccgccgc
     1801 cgccgatcat cagtccgaac cgccagtgcg cgccgattgc acggcagaag ctctccacaa
     1861 cgcgcagcgc ttcgccgttg atttcgtcct cgggaaagcc gcagttgacc atcgcgtaga
     1921 cgcgggggac tttgccggtt ctttcctgcg atttcacaaa cgcggcgtag tcctgcaaga
     1981 accgggtcaa cagacctggc aggcagaaga agtacagcgg aaagattata agaattgcgt
     2041 cggattccag tattgcggta tacgcttccg ctgagccgct gagtacagat tttctcgcgt
     2101 tgcagcgctc aatactgatt tcgtcgcctg caatccgcgt ttccgccagt aaggaaagat
     2161 actccgaaac ggaagggttg ctatccggc
//
LOCUS       DAOMGY010000272         2185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_56417_length_2185_cov_3.557746, whole genome shotgun sequence.
ACCESSION   DAOMGY010000272 DAOMGY010000000
VERSION     DAOMGY010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56417_length_2185_cov_3.557746"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PLR57_04940"
     CDS             <1..923
                     /locus_tag="PLR57_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQQ40835.1"
                     /translation="MLGTKAANTGLSGADLLIAFGSRFNDRVTGDASRFGKNAQIVQI
                     DIDEAEINKNIRTFRSLLGDVKTVLGKLLPLLALSPESLAAREAWLGEIQGLKLNGQR
                     VAACDLGPKAVMEAIFERSPKDTFIVTDVGQHQLWAAKNYSYDEPGSFISSGGFGTMG
                     YGAGASIGARVGRPDRRIILITGDGSFRMNCNELATMEHYGLPVIIVVMNNGVLGMVR
                     QWQTVFYGKRYSQTNLDRGPDFCKLAEAYGLKGACAHSLEEFERAFEEALTSETATVI
                     DCHIGCDETVAPMVAPGKPITDFIIGEQHA"
     gene            948..>2185
                     /locus_tag="PLR57_04945"
     CDS             948..>2185
                     /locus_tag="PLR57_04945"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQQ40836.1"
                     /translation="MISDNVKTNAERAPHRSLFKAMGYTDEELQRPLIGIANPKSEIV
                     PGHMHLGQLAEAVKAGIRMAGGTPFEFGMIGVCDGIAMGHAGMRYSLASRELIADSCE
                     AMAMAHGFDGMVFLPGCDKIIPGMLMAAARLDLPAIVLTAGPMLSYKAEDGRQLDLNS
                     VFEAVGAFKAGMATAEELAYCENNACPGCGSCSGMFTANSMACLSEAVGMALPGGGTI
                     PAVYAERVRLAKYTGMRIVDLVKKDLRPSQILTERAFYNALAVDMALGCSTNSVLHLT
                     AIAAEAGVPIRLETVNEISAKVPNLCRLAPSGPHHVEDLYHAGGVSAVIKELMDNGFF
                     DGSALSVSGGSMAESIANARVRNRTVIRSCSEPYSKTGGIAVLKGNLAPDGAVVKRAA
                     VLESMLVHSGPAKVFDSEESA"
BASE COUNT          446 a          665 c          685 g          389 t
ORIGIN      
        1 gcatgctcgg cacaaaggcg gccaataccg gactgtccgg cgcggatctc ctaatcgctt
       61 tcggcagccg gttcaacgac cgcgttaccg gcgacgcgag cagattcggc aaaaacgccc
      121 agatcgtgca gatcgatatt gacgaagcgg agatcaataa aaacatccgc acgttccgct
      181 cgcttttggg ggacgttaaa acggtgctcg gcaagctcct gccgctgctt gcgctttctc
      241 cggaaagtct tgccgcgcgg gaagcctggc tcggcgaaat ccaggggctg aagctgaacg
      301 gacagcgcgt cgcggcctgt gatctcggcc cgaaagcagt aatggaagcg atcttcgagc
      361 gcagcccgaa ggacacgttc atcgttaccg acgtcggcca gcaccagctt tgggcggcca
      421 agaattattc ctacgacgaa ccgggctcct ttatctcctc cggcggattc ggcacgatgg
      481 gttacggcgc gggcgcttcc ataggcgcgc gggtcggacg gcccgaccgc cgcatcatcc
      541 tgattacggg cgacgggagc ttccgcatga actgcaacga gctggccacc atggagcact
      601 acggtttgcc ggtgattatc gtggtcatga acaacggcgt gctcggcatg gtgcgccagt
      661 ggcaaacggt gttctacggc aagcggtatt cccagaccaa tctcgaccgc gggccggact
      721 tttgcaaact cgcggaggca tacggcctga aaggcgcatg cgcgcattcg ctggaagagt
      781 ttgagcgggc attcgaagag gcgctcacgt ccgaaacggc aacggtgatc gactgtcata
      841 tcggctgcga cgaaacggtc gcccccatgg tcgcccccgg caagccgatt acggacttta
      901 tcatcggcga acagcacgcg tgataatagg aacggagaag acgaaatatg atcagtgaca
      961 atgtgaaaac caacgcggag cgcgcgccgc accgctcgct gtttaaagcc atggggtata
     1021 cggacgaaga actgcagcgc cccctgatcg gcattgccaa cccgaaaagc gaaatcgttc
     1081 ccgggcatat gcatttgggt cagctcgcgg aagcggtcaa ggcgggtatc cgcatggcgg
     1141 gcggaacgcc gtttgagttc ggtatgatcg gcgtttgcga cggcatcgcc atggggcatg
     1201 cgggcatgcg ctactcgctc gcctcgcgcg aactgattgc cgacagctgc gaagcaatgg
     1261 ccatggcgca cggcttcgac ggcatggtat ttctgccggg atgcgacaag atcattcccg
     1321 gcatgctcat ggcggcggcg cggctcgacc tgcccgccat cgtgctgacg gcaggcccga
     1381 tgctctccta caaagccgag gacggcagac agctcgacct caacagcgtg ttcgaagcgg
     1441 tcggcgcgtt caaagccggc atggctacgg cggaggagct ggcgtactgc gaaaacaacg
     1501 cctgccccgg ctgcggctcc tgctccggca tgtttacggc aaactccatg gcgtgccttt
     1561 cggaagccgt cggcatggcg cttccgggcg gcggcacgat tccggcggta tacgccgagc
     1621 gcgtccgcct tgccaagtac acgggcatgc ggattgtcga tctggtgaaa aaagacctgc
     1681 gccccagcca aattctcacc gaacgcgcgt tttacaacgc actcgccgtg gatatggcgc
     1741 tggggtgttc caccaactcc gttctgcacc taacggcgat tgccgccgag gcaggcgtgc
     1801 cgatccggct ggaaacggtg aacgaaatta gcgcgaaggt gccgaatctc tgccgcctcg
     1861 cgccgtccgg cccgcatcac gtagaagacc tttaccatgc gggcggggtt tccgccgtga
     1921 tcaaagagct catggacaac ggattctttg acggctccgc gctgagcgtt tcgggcggtt
     1981 cgatggcgga atctattgca aacgcccgcg tccgaaaccg caccgtcatc cgcagctgca
     2041 gcgaaccgta ttccaagacg ggcggcatcg ccgtgctgaa aggcaacctc gccccggacg
     2101 gagccgtggt caagcgcgcg gccgttctgg agagcatgct cgttcattcc ggcccggcaa
     2161 aggtgttcga ctccgaagaa agcgc
//
LOCUS       DAOMGY010000273         2178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_56699_length_2178_cov_3.712671, whole genome shotgun sequence.
ACCESSION   DAOMGY010000273 DAOMGY010000000
VERSION     DAOMGY010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56699_length_2178_cov_3.712671"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1093)
                     /locus_tag="PLR57_04950"
     CDS             complement(152..1093)
                     /locus_tag="PLR57_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HQQ40837.1"
                     /translation="MELQRNSAIDRFRGLAVLMMAVANYLGEVEIVPAWLKHAPDIGF
                     TITDTIASIFLFAVGLLYGRSFRKRAGINKSAAYGHFATRYLALIGIGAFLSAGGDVL
                     AGIHAEWGVLQSIGVAGLFCLAFIQLKPVPRMLAGLALLTAYQFMLDRSMLEAVLNTP
                     HGGIFGAISWSAMLIMVSGLGDLKERGLKEYTLGSLAVSALAAAAAFLTPISKNRVSA
                     SFILLTLAISAWLYLAIELLTRNWKTRGALCWVGENPLGLYVLHMLMLGLLAVPQNPH
                     WYAGAPVWLVAVQLTVFLGILIAAAYLLHRKNIVIKL"
     gene            complement(1169..>2178)
                     /locus_tag="PLR57_04955"
     CDS             complement(1169..>2178)
                     /locus_tag="PLR57_04955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQQ40838.1"
                     /translation="GIFLSLLLGTAILVICVNSGSRLSFYLALLFGLSALIGAALFFN
                     LRGKYKSAAWLTTICMVLGPWFSILLDPAVVAGDFVPLVYIGISIQLCSILLSERATI
                     GIALVQMSGLIAFILFSPVLRAINWPSLVAFIAFTATIGTLAGFSNRKQLEQIEQQRN
                     QLQRDEAKLRELSVHDPLTGLYNRRYMEETLDREIKRALRKGRSLAVVMTDIDGFKAI
                     NDTFGHVPGDMLLVRVAKFLMKGVRASDVVCRYGGDEFVLILPECSLEEAILRVSEMR
                     IDVEKMPLGEEVAVKEPLTMSFGVAAFPENGTTLEALIEAADRALYASKRAGRNRVNG
                     N"
BASE COUNT          499 a          653 c          594 g          432 t
ORIGIN      
        1 atacttctgt aaatccgggt gcacgggata tttcgtcata tatacctgct ttctttcaaa
       61 gggattgttt tttattcgat gaatcagtca ggaccacccg caaaacgtgt ggccctgact
      121 gatgttttat cactccagca ggagtgcccg cctatagctt gatgacgatg ttctttctgt
      181 gcagcagata ggccgccgca atcaggatgc cgaggaatac cgtaagctga accgccacga
      241 gccaaacggg agcgcccgcg taccaatgcg gattctgcgg cacggcgagc aggccgagca
      301 tcagcatatg caggacgtaa agccccagcg ggttttcgcc cacccagcag agcgcgccgc
      361 gcgttttcca gttccttgtg aggagttcaa tcgccagata cagccaggcg gagattgcca
      421 gcgttaaaag tataaaactc gccgaaacgc ggtttttgct gattggcgtt aaaaacgccg
      481 cggctgccgc aagcgcggat acggcgaggc tgccgagcgt atattccttc agccctcttt
      541 ctttgagatc gcccagcccg cttaccataa tcagcatggc gctccaggag attgcgccga
      601 aaatgccgcc gtgcggcgta ttgagcacgg cttccagcat ggatcggtcg agcatgaact
      661 ggtatgccgt cagcagcgcg agcccggcaa gcatgcgcgg aaccggcttg agctgaataa
      721 acgcaaggca gaacagccca gcaacgccga tggactgcaa aacgccccac tccgcgtgaa
      781 tgcccgcgag gacgtccccg ccggcggaga gaaacgcgcc aatgccgatc agcgccagat
      841 accgcgttgc aaaatggccg tatgctgcgc tcttattgat gccggcacgc ttgcgaaacg
      901 atctgccgta gagcaaccct actgcaaaga ggaagatcga agcgatggtg tccgtaatcg
      961 taaacccgat gtccggcgcg tgcttgagcc acgcgggaac gatttccacc tcgccgaggt
     1021 agtttgccac cgccatcatc agcaccgcga gcccgcggaa acgatcgatt gcgctgttgc
     1081 gctgcagttc catactgtac ccgcctcccc gtattcgttt ttgtttgcgt acctgtattc
     1141 tcgagccgct tcgttattcc cgtgcccgct agttgccgtt gacgcggttt ctgcccgcac
     1201 gtttagaagc gtagagcgcc cgatccgccg cctcgatcag tgcttccaga gttgttccgt
     1261 tttccgggaa cgctgcaacg ccaaatgaca tcgtcagcgg ctccttgacg gcaacttcct
     1321 cgcctaacgg catcttctcc acgtcgatac gcatttcgga aacgcgcaga atcgcttctt
     1381 ccagcgagca ttccggaagg atcagcacga actcatcccc gccatagcga catacaacgt
     1441 ccgacgcgcg aacgcccttc atcaggaatt ttgcgacgcg cacaagcagc atatcccccg
     1501 gcacatggcc gaaggtatcg ttaatcgcct tgaacccgtc gatatccgtc atgacgaccg
     1561 caagcgagcg gcctttgcgc agcgcgcgct tgatttcgcg atcgagtgtt tcttccatat
     1621 aacgacggtt atacagcccc gtcagcgggt cgtgcacaga gagctcgcgg agctttgctt
     1681 cgtcgcgctg gagctggttt cgctgctgtt caatctgttc gagctgcttg cggttggaaa
     1741 accccgccag cgtgcctatg gtcgccgtaa aagcgataaa cgcaacgaga ctcggccagt
     1801 tgatggcgcg cagcaccggg ctgaacagaa taaacgcgat cagcccgctc atctgaacca
     1861 gcgcaatgcc gatggtcgcg cgctctgaga gcaaaatcga gcagagctgg atagagatgc
     1921 caatatagac cagcggcaca aagtcccccg ccacgacggc agggtccagc agtatggaga
     1981 accacgggcc aagcaccatg cagatggtcg tcagccaggc ggcgcttttg tatttccctc
     2041 tcagattgaa aaacagcgcg gcgccgatga gcgccgaaag cccgaagagc agcgcaagat
     2101 aaaacgagag ccgcgacccg ctgttgacgc agataacgag aatggcagta cctaataata
     2161 acgatagaaa aatcccct
//
LOCUS       DAOMGY010000274         2175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_56820_length_2175_cov_3.140566, whole genome shotgun sequence.
ACCESSION   DAOMGY010000274 DAOMGY010000000
VERSION     DAOMGY010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56820_length_2175_cov_3.140566"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1001)
                     /gene="murI"
                     /locus_tag="PLR57_04960"
     CDS             complement(156..1001)
                     /gene="murI"
                     /locus_tag="PLR57_04960"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQQ40839.1"
                     /translation="MDTRPIGVFDSGMGGLTAVRQMIRMLPGEDIVYFGDTGRVPYGG
                     RSVETIVRYARQDVAFLRSFDLKAIVIACGTVSTTALDVIIEENHIPILGVVEPAARA
                     AAEATKNGRVGVVGTKATIRTGAYEASIRKLLPEVYLTAKACPLLVPLVEEGRVQRGD
                     LVAERVVEEYLSPVKAQGVDTLVLGCTHYPLLKDIISDYMGPTVTLIDTGEQAARAIA
                     ALLNERDQLNDRTRNGERRYYASDSAEDFAATASLYLGSSIAGSVERIDIELYLNKGW
                     HSTKA"
     gene            complement(1173..1598)
                     /locus_tag="PLR57_04965"
     CDS             complement(1173..1598)
                     /locus_tag="PLR57_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HQQ40840.1"
                     /translation="MNQSDKKCVMVIDANLPLGVIANTSAILGVSIGKQAPQAVGQDV
                     LDASGKTHPGIITVPITVLKGDEALLKNLRAKLYEPEYAEMQVVDFSNVAQSCNVYEE
                     YVASAAETPEREHTYYGVAIYGDKKKVNRLTGYLPLLRA"
     gene            complement(1582..>2175)
                     /locus_tag="PLR57_04970"
     CDS             complement(1582..>2175)
                     /locus_tag="PLR57_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQQ40841.1"
                     /translation="EITGADEPPVFTTPVVYRSELTETLYDLHGRIMRSETGFGKEEL
                     FFFFLAQLLTEYANSGEAPEEPVGNGAIEAVCAFLEQNYEKPVSLDALGERAGYSKYH
                     LLRSFTKQKGISPYSYLETVRIGKVKELLQAGVLPAEAAARTGFSDQSHLGNVFKRYI
                     GLTPKQYWRIFAQERVNTDSVASAGHTNGETNHESIG"
BASE COUNT          430 a          673 c          583 g          489 t
ORIGIN      
        1 cgccggctgc accgcggcgc agctcgttac cctcgcggac gcctgcctct accgagccaa
       61 agagcgaggg cgcgactgcg cggaaacatg cactctgtaa ataaaaaact gccgtcaggc
      121 agtttttgat ttcacattgc tgatttttta cccggctacg ccttagtcga atgccagcct
      181 ttatttaagt acagctcaat atcgattcgc tcaacgcttc cggcgatgct cgaacccaaa
      241 tagagcgaag cagtggcggc gaaatcttcc gcgctgtcac tggcgtagta gcgccgctcg
      301 ccgtttcttg tgcggtcgtt gagctgatcc cgctcgttaa gcagcgcggc aatcgcgcgc
      361 gccgcctgtt cgccggtgtc gatcagcgtg acggttggcc ccatgtagtc cgaaatgatg
      421 tccttgagca gcggataatg cgtgcagcca agcaccagcg tatccacgcc ctgcgccttg
      481 acgggagaaa ggtactcttc caccacgcgc tcggcaacga gatccccgcg ctgaacacgc
      541 ccttcctcca ccagcggaac aagaagcggg caggctttcg ccgtgaggta gacttccggc
      601 aggagctttc ggatgctggc ctcatatgcg ccggtgcgga tggtcgcctt tgtgccgaca
      661 acgccgacgc ggccgttctt cgtggcttcc gccgccgctc tggcagcggg ttcgaccacg
      721 ccgagaatcg gaatatggtt ttcttcaatg atgacatcca gcgccgtcgt ggaaaccgtt
      781 ccgcaggcga tgacgattgc tttgaggtcg aaactgcgca gaaacgcaac gtcctggcgc
      841 gcataccgca caatcgtctc cacggaacgc ccgccatagg gcacgcgccc ggtatcccca
      901 aagtagacga tatcctcgcc gggcagcatg cggatcatct ggcgaaccgc cgtcaatccg
      961 cccatgccgg aatcgaaaac gccgatgggt cttgtgtcca tgctgttcct ccgcgaaaaa
     1021 aagcgggcaa gcccggcctg cccgtctgtc agtgtccttg cggaacacat tgtatcacat
     1081 tattggcgat cgataaagaa acctatcatt cctttctatc ggtatcacat tattgcattt
     1141 cgataaagaa acgcattctt cggttgagca cgctacgccc tcagcagcgg cagataaccc
     1201 gtcaggcggt tgaccttctt tttgtcgccg taaatcgcaa caccgtaata cgtgtgctcc
     1261 ctctccggcg tctcagcggc gctggcgacg tattcctcgt acacgttgca gctctgcgcc
     1321 acgttggaaa agtccaccac ctgcatttcc gcgtattccg gctcgtagag cttggcgcgg
     1381 aggtttttga gcagcgcttc gtcgcctttg agcacggtaa tcggcacggt gatgatgccc
     1441 ggatgcgtct ttccggaggc gtccagcacg tcctgtccta ctgcctgcgg cgcctgcttg
     1501 ccgatggata cgccgaggat ggcggacgta ttggcgatca cgcccaacgg aaggttcgcg
     1561 tcgatcacca tcacgcactt tttatccgat tgattcatga tttgtttctc cattcgtatg
     1621 tccggcgctt gccacagaat cggtattcac ccgctcctgc gcaaagatac gccagtactg
     1681 cttgggcgtc agcccgatgt atcgcttaaa gacgttgccc agatggctct gatcggagaa
     1741 cccagttcgt gccgccgctt ccgcgggcag tacgcccgcc tgcagcagct cttttacttt
     1801 gcctatccgc accgtttcca aatagctgta cggcgaaatc cccttctgct ttgtaaacga
     1861 acgcaacaga tgatatttgc tgtagcctgc gcgttcgccg agcgcgtcga gcgacacagg
     1921 tttctcatag ttctgctcca gaaacgcgca aaccgcttcg atcgcgccgt ttccaacggg
     1981 ttcttccggc gcttcgccgc tgttggcata ttccgtcagc agctgcgcca aaaaaaagaa
     2041 aaacagttcc tctttgccaa accccgtttc ggagcgcatg atacgcccgt gcagatcgta
     2101 cagcgtttcc gtgagttcac tgcggtagac gaccggcgta gtaaagacgg gcggctcgtc
     2161 ggctccggta atctc
//
LOCUS       DAOMGY010000275         2171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_56980_length_2171_cov_3.440454, whole genome shotgun sequence.
ACCESSION   DAOMGY010000275 DAOMGY010000000
VERSION     DAOMGY010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56980_length_2171_cov_3.440454"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PLR57_04975"
     CDS             <1..862
                     /locus_tag="PLR57_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HQQ40842.1"
                     /translation="NTLNRFASPEMQQEIRLEMRMLTKQIEAAQKRVEAMNFETRKNV
                     LRYDDVMNKQREIIYGQRRRVLLGEDLSESIRTMVRQTIDSIVETACNPHAPRESWDF
                     TALSIEACKLCCRQPKPLFTADEQKKFGISEIKTRLYEYSDTVYAETEQHLKSAEVDM
                     RENERVILLRTVDEHWMDHIDAMDQLKQGIGLRSYAQKDPVYSYTSEGFEMFDAMVAE
                     IREQTVRRLYQMQVTGGPLRRVQLAKPIEPKTDGTSSTFSRSDKKVGRNDPCPCGSGK
                     KYKNCHGKNE"
     gene            1004..2104
                     /gene="prfB"
                     /locus_tag="PLR57_04980"
     CDS             1004..2104
                     /gene="prfB"
                     /locus_tag="PLR57_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_122013059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQQ40843.1"
                     /translation="MIPLDEYKMNLDAFEEQLDECRTALNPEQIKKELADLDALMGAP
                     DFWNDVENANKMNKQVKQLQHKLERFQKLRGRFEDLRTLIEMAEEEQDDSLLTEIKTE
                     TAAVSSGIEALRLEQMLKGPYDNSNAVLSLHAGAGGTEAQDWTEMLYRMYTRYCETCG
                     WTVRELDVLDGDDAGIKSVTFEVDGENAYGYLKAEKGVHRLVRISPFDSSGRRHTSFS
                     SLDVTPIFDDDSNKIEIDPDDLRVDTYRSSGAGGQHVNKTSSAIRITHLPTGIVVQCQ
                     NERSQIQNRETAMRMLRGKLLELQEREREAHMAEIKGEMKKIEWGSQIRSYVFQPYTL
                     VKDHRTGVENGNIQAVMDGDLEPFIHAYLVQS"
BASE COUNT          555 a          628 c          617 g          371 t
ORIGIN      
        1 taacacgctc aaccggttcg cttcccccga gatgcagcag gagatccgtc tcgaaatgcg
       61 gatgctcaca aagcagatcg aagccgcgca gaagcgcgtg gaagccatga actttgaaac
      121 gcgtaaaaac gttctgcgct acgacgacgt tatgaacaag cagcgtgaaa tcatctacgg
      181 ccagcgccgc cgcgtgctgt tgggcgaaga cctttccgaa agcattcgca ccatggttcg
      241 ccagacgatc gacagcattg tggaaaccgc ctgcaacccg cacgccccgc gcgagagctg
      301 ggatttcacc gcgctttcca tcgaagcctg caagctctgc tgccgccagc ccaagccact
      361 gtttaccgca gatgaacaga agaaattcgg catcagcgag attaaaaccc gcctgtacga
      421 gtacagcgac acggtttatg ccgaaaccga gcagcatctg aaatccgcgg aagtcgatat
      481 gcgcgaaaac gagcgcgtca tcctgctgcg cacagtggac gaacactgga tggatcacat
      541 cgacgccatg gatcagctca aacagggcat cggcctgcgc tcttacgcgc agaaggaccc
      601 cgtatactct tacacgagcg aagggtttga aatgttcgac gcaatggttg cggaaatccg
      661 cgagcagacc gtccgcaggc tctaccagat gcaggtgacc ggcggcccgc tccgccgcgt
      721 tcagctggca aagccgatcg agcccaaaac ggacggaacc agctccacgt tctcccgcag
      781 cgacaagaag gtcggcagaa acgatccctg tccctgcgga agcggcaaga aatacaagaa
      841 ctgccacggc aaaaacgaat gactccacag cgcgccgggc gaatctccgg gtgcgggata
      901 gcgggtataa cagctgcttt gccccggagc cgcgccgcaa aaggccaatc tcctgcgacg
      961 cgaaaatacc caatatggta cgattgagag gaatcgaaac actatgatcc cgttggatga
     1021 atataaaatg aatctcgatg cgtttgagga gcagctcgac gaatgccgaa ctgcgctcaa
     1081 cccggagcag attaagaaag agttagccga tctggatgcg ctcatgggcg cgccggactt
     1141 ctggaacgac gttgagaacg cgaacaagat gaacaagcag gtcaagcagc tgcagcataa
     1201 actcgaacgc ttccaaaagc tgcgtggccg gttcgaagac ctcagaaccc tgatcgagat
     1261 ggccgaggaa gagcaggacg acagcctgct taccgaaatc aagaccgaaa ccgccgctgt
     1321 ttccagcggc attgaggcgc tccggctcga gcagatgctc aaaggccctt acgacaattc
     1381 caacgccgtt ctctcgctcc acgcgggcgc gggcggcacc gaggcgcagg actggacgga
     1441 gatgctctac cgcatgtata cgcgctattg cgaaacctgt ggctggactg tgcgcgagct
     1501 ggacgttctg gacggcgacg acgcgggcat taagtccgtt accttcgaag tggacggcga
     1561 aaacgcctat ggctacctca aagcggaaaa gggcgtgcac cggcttgtgc gcatttcgcc
     1621 tttcgattcc agcggcaggc ggcatacgtc gttttcttcg ctggacgtaa ccccgatttt
     1681 cgacgacgac tcgaacaaga ttgaaatcga cccggacgac ctccgcgtgg acacctaccg
     1741 ctccagcggc gcgggcgggc agcacgtcaa taagaccagc tccgccatcc gcattacgca
     1801 cttgcctacg ggcatcgtcg tgcagtgcca gaacgagcgc agccagattc agaaccgcga
     1861 aaccgcaatg cgcatgctgc gcggcaagct gttggaactg caggagcgcg aacgcgaggc
     1921 gcacatggcc gagatcaagg gcgagatgaa gaagatcgag tggggcagcc agatccgcag
     1981 ctatgtgttc cagccgtaca cgctggtcaa ggatcaccgc acaggcgttg aaaacggcaa
     2041 cattcaggcc gttatggacg gcgatcttga accgttcatc cacgcgtatc tggtgcagtc
     2101 gtaagaactc cactttcttt gaaaagaaag tggacaagaa acttatatgt agctgacatg
     2161 aagttctttt g
//
LOCUS       DAOMGY010000276         2169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_57050_length_2169_cov_3.448912, whole genome shotgun sequence.
ACCESSION   DAOMGY010000276 DAOMGY010000000
VERSION     DAOMGY010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57050_length_2169_cov_3.448912"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLR57_04985"
     CDS             complement(<1..769)
                     /locus_tag="PLR57_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQQ40844.1"
                     /translation="MPHGEHKGRLTGMILLLSASIVWGVAFVAQSAGMAYMGPFTFTA
                     VRSAIAALGLWGLVALFDRLGLSPKNTDKKLLWRAGLILGVVMTIASNLQQIGLVTTS
                     AGKSGFITSLYIVFVPIFGLFIKRRPHPMLWACVVLAAVGLYFLSIRGDFTMSLGDLS
                     VLGCAVVFTFQILLVDHYAPKVDIMRLNCIQFTVMALLSAIPMVLFEEPKLNSILDSW
                     ITIVYAGLFSGCVAYSLQMFGQRRVEPTTASLLLSLEA"
     gene            961..2085
                     /locus_tag="PLR57_04990"
     CDS             961..2085
                     /locus_tag="PLR57_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HQQ40845.1"
                     /translation="MQPSKVYFTNMRTSYENSLPEKLARLIKKAGIETIDFADRFVAI
                     KVHFGEPGNLSFIRPNFAYSVAEVIRARGGKPFLTDCNTLYTGSRKNALDHLEAAARN
                     GFSSVSTGCQIIIADGLKGLDEALVPVEGGKHITQAKIGQAIMDADIFVSLTHFKGHE
                     GVGFGGALKNIGMGSGSRAGKMEQHCDGKPEVDSALCRGCRQCGKQCAHGAITYHGEK
                     RIASIDHALCVGCGRCIAACNFDAISNEGGNSDRILGEKIAEYSLAVLQNRPSFHINL
                     VMQVSPNCDCHAENDMPIIPDVGMFASFDPVALDQACADACNRQPIMPNSQLADHLAA
                     GEHHHDHFTDSAPGTDWTVALAHAEELGIGTRQYELITVK"
BASE COUNT          540 a          659 c          584 g          386 t
ORIGIN      
        1 ccgcttccaa actcagcagc aagctcgcgg tcgtaggctc cacgcggcgc tgaccgaaca
       61 tctgcagaga atacgcaacg cagccggaga acagccccgc atatacgatg gtaatccacg
      121 aatcaagaat gctgttcagc ttcggctctt caaacagcac catgggaatg gcggaaagca
      181 gcgccatcac cgtaaactgg atgcagttga gccgcatgat gtccactttt ggcgcgtagt
      241 gatcgaccaa caaaatctga aacgtaaaca cgaccgcgca gccgagcacg gagaggtcgc
      301 caaggctcat cgtaaaatcg ccgcgaatcg agaggaaata aagccccacc gccgcgagca
      361 cgacgcaagc ccagagcatg ggatgcggcc tgcgcttgat gaacagtccg aagatgggca
      421 cgaacacgat gtaaagcgac gtgatgaacc ccgattttcc ggcggaagtc gtgaccagcc
      481 cgatctgctg aaggttgctg gcaatggtca tgaccacgcc gagaatcaga cccgcgcgcc
      541 agagcagttt tttgtcggta ttcttcgggc taagccccaa tcggtcgaac agcgccacaa
      601 gcccccataa cccgagggcg gcaatcgcgc tgcgcaccgc ggtaaacgta aacggcccca
      661 tataagccat gcccgcgctc tgcgcaacaa acgcaacgcc ccatacgata gaagcgctca
      721 acagcagtat cattccggtc agccggcctt tgtgttcccc gtgtggcata aaaagattca
      781 tccaatccgg cgcccatccg cgccaaaagc aaacattcca agacgaaatc atactcgttt
      841 ttcactaaaa acgcaagcgt tccccgttcc gatggcacgg ggatattgtg cgtttgtgcg
      901 gcggtatggt attctttgaa tattccggca gaagaagccg tcagaacagg agaaaaaaag
      961 atgcagcctt ccaaggttta ttttacgaat atgcgtacaa gctacgaaaa ttcgctgccc
     1021 gaaaagctcg cgcggcttat aaaaaaagcg ggaatcgaaa ccatcgattt tgcggatcgc
     1081 tttgtcgcca tcaaggtgca tttcggtgag ccgggcaacc tttcgttcat ccgccctaac
     1141 tttgcctact ccgttgcgga agtcatccgc gcacgcggcg gcaagccgtt tttaaccgac
     1201 tgcaacacac tctacacggg cagccgcaaa aacgcgctcg accatctgga ggccgccgcg
     1261 cgtaacggtt tttccagcgt ctccaccggc tgccagatca tcattgccga cggcctcaag
     1321 ggactggacg aagcgctcgt gccggttgag ggtggaaagc atatcacgca ggccaagatt
     1381 ggccaggcga ttatggacgc ggacatcttc gtatcactga cgcatttcaa aggccacgag
     1441 ggcgtcggct tcggcggcgc gctcaaaaac atcggcatgg gcagcggctc gcgcgcgggc
     1501 aagatggagc agcactgtga cggcaagcct gaagtcgatt ccgctctctg ccgcggctgc
     1561 cgccagtgtg gtaaacagtg tgcgcacggc gcgattacct atcacgggga aaagcgcatc
     1621 gccagcatcg accacgccct ctgcgttggc tgcgggcgct gcatcgccgc ctgcaacttc
     1681 gacgcaatct ccaacgaagg cggaaacagc gaccgcatcc tcggcgaaaa gattgcggag
     1741 tactcgctcg cggttctgca gaaccgcccg agcttccata tcaatctcgt gatgcaggtt
     1801 tcccccaact gcgactgcca tgcggaaaac gacatgccga tcattccgga cgtaggcatg
     1861 ttcgcctcgt tcgacccagt tgcgctcgat caggcctgcg cggacgcctg caacaggcaa
     1921 cccatcatgc cgaacagcca gcttgccgac catctggccg cgggcgaaca ccaccacgac
     1981 cactttacgg attccgcgcc cggcaccgac tggacggtcg cgctggcgca cgccgaagag
     2041 ctcggaatcg gtacgcgcca gtatgaactg atcactgtaa agtaaactgc ctgacaacgt
     2101 aaaatgcccg ctgatgaact gaaccccgga aaacagacat tgaaagaaaa gagcctccta
     2161 tcgtaggat
//
LOCUS       DAOMGY010000277         2167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_57144_length_2167_cov_3.074811, whole genome shotgun sequence.
ACCESSION   DAOMGY010000277 DAOMGY010000000
VERSION     DAOMGY010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57144_length_2167_cov_3.074811"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..815
                     /locus_tag="PLR57_04995"
     CDS             63..815
                     /locus_tag="PLR57_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40846.1"
                     /translation="MKKVTSILSKRILPALAAVLLLVVFSVSALAESNKTDRGSGKPD
                     KTTSQRGNSDKDNGNGQEKHEEKVRNTYRGISVEKIALAIDSVTDEATKLELTALLEA
                     YMTALENKDVALSTKDGSLSELSQLASAARAALKTGLEDAGFTLGSVLGWQEWKDYGN
                     TALDLEAIAAAISALDDTDENKAALGALLAAYQDALNAAEAGSEEDGEALNEALEAAR
                     DGLLEALYETGIFPLQEAVPEELPEATPEVTE"
     gene            1329..>2167
                     /locus_tag="PLR57_05000"
     CDS             1329..>2167
                     /locus_tag="PLR57_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQQ40847.1"
                     /translation="MNLNKQKRAPIYEALAQFRKQRVVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLCHPVSVIREAEELAADAFGATHAFLMVGGTTSSVQSMILS
                     ACKSGDKIILPRNVHKSAINALVLCGAVPVYVDPRVDPKLGIPLGMEFADVQSAIEQN
                     PDAVAVLVNNPTYYGVCSNLKAIVDLAHAHGMQVLADEAHGTHLYFSENLPLCAMEAG
                     ADMAAVSMHKSGGSLTQSSLLLLGPNVNARYVDKIINLTQTTSASYLLMSSLDVSRRN
                     LALR"
BASE COUNT          511 a          584 c          652 g          420 t
ORIGIN      
        1 tgcggcagac cgggcatcca cccgcaccgc gcaaggctca gtttgaaagg agcagatttg
       61 aaatgaaaaa ggtgacaagc attctttcga agcgtatcct tccggcgctg gcggcggttc
      121 tgctgctggt tgtgttcagc gtatccgccc ttgctgaatc caataagacg gatcgcggtt
      181 ccggcaagcc cgacaagacc acgagccaac gcgggaattc agacaaggac aatggaaacg
      241 gccaggagaa gcacgaggag aaggtgagaa acacctaccg tggcatctcc gttgagaaga
      301 tcgcgcttgc cattgacagc gtaaccgacg aagcaaccaa gctggagctg accgcgctgc
      361 tggaagcgta tatgacagcc cttgagaata aggacgttgc gctttccacc aaggacggca
      421 gcctgagcga gttgtcccaa ctggcgtccg ccgcgcgcgc cgcgctgaag accggccttg
      481 aggacgcggg ctttacgctt ggcagcgtgc tgggctggca ggaatggaaa gattacggta
      541 acaccgcgct tgatttggaa gcgattgccg ccgccatctc cgcgctggac gataccgacg
      601 agaataaggc tgcgctcggc gcactgctgg ccgcttatca ggacgctctg aacgcagccg
      661 aagccggttc ggaagaggac ggggaagcgc ttaacgaggc tctggaagcc gcgcgtgacg
      721 gcctgctgga agcgctgtat gaaaccggca tcttcccgct gcaggaagcc gttccggaag
      781 aactgcccga agcgacgccg gaggttacgg aataacgcag ctttgctggc gagactgttg
      841 accaagaacg agaacgtatg ttttattcgg gctgttcctg tataaaatcg ggagcagccc
      901 gaaaaatata aattgacata cgggacaaat atgttaagat agataagcgt gaatcggctc
      961 tgccggtttg cgggacttgg ccgagtaaaa atgcccatcg gacagggctg gccgccgccg
     1021 cgcgtgacgt gaagcgtcac attattgatt gagttttaag actcaaattt tataagttgg
     1081 ttacttcata accagtgcgt tacagcacac cacttgtgaa acggtcaata agagtagcga
     1141 cggcatccat gctctacaaa actctgatga aacggacata gtcatcgttc tatccaactc
     1201 gcgcgcagtg tttgcgcacg ccggaaaacg gaacagagag atgacgacag gtggtagaat
     1261 ttcgccgcct gtctttttgt tttggcggcg aactggtaaa gcgtagacca gcggaaggaa
     1321 cgatgccgat gaatttaaat aagcagaagc gcgcgcccat ctatgaggca ctcgcccagt
     1381 tccgtaaaca gcgggtcgta ccgttcgacg tgccggggca taagcgcgga cgcggcaatc
     1441 cggagctggt ggaactgctg ggcgaaaagt gcgtaggcat agacgtcaac tccatgaagc
     1501 cgctcgataa cctctgccat ccggtttccg ttatccgcga agcggaagag ctcgcggcgg
     1561 acgcgtttgg cgcgacgcac gcatttctaa tggtcggcgg cacgacttcg agcgtacaga
     1621 gcatgattct ctcggcctgc aagagcggcg ataaaatcat cctgccgcgc aacgtgcata
     1681 aaagcgcgat taacgcgctc gtgctttgcg gcgcggtgcc ggtgtatgtt gatccgcgcg
     1741 tcgatcccaa gctcggtatt ccgcttggca tggagtttgc ggacgtgcag tccgccatcg
     1801 agcagaaccc ggacgcggtg gcggttcttg tcaacaaccc gacgtattac ggcgtttgtt
     1861 ccaacttaaa agccatcgtg gatctggcgc acgctcacgg catgcaggtt ctggcggacg
     1921 aagcgcacgg aacgcacctc tacttcagcg aaaacctgcc gctttgcgcc atggaagcgg
     1981 gggcggacat ggcggcggtc tccatgcata aatccggcgg gagcctgacg cagagctcgc
     2041 tcttgctgct cgggccaaac gtcaacgcgc ggtacgtgga taaaattatc aacctgacgc
     2101 agaccacgag cgcgtcgtac cttttaatga gcagcctcga cgtttcccgg cgaaaccttg
     2161 cgctgcg
//
LOCUS       DAOMGY010000278         2161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_57392_length_2161_cov_2.788224, whole genome shotgun sequence.
ACCESSION   DAOMGY010000278 DAOMGY010000000
VERSION     DAOMGY010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57392_length_2161_cov_2.788224"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            374..1156
                     /locus_tag="PLR57_05005"
     CDS             374..1156
                     /locus_tag="PLR57_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ40848.1"
                     /translation="MMFSPLCSGSSGNASYLEAGGVRLLVDAGLPGKRMEQLLDMIDV
                     SARSLDAILVTHEHSDHVSGVGVLSRKYDIPVYAAEACWRSLPASLGAFAPRNVRVFE
                     PDQDFYLKQLCVHPFTIPHDSAHPVGYTFVHDGRKLAIMTDIGHVSNTMLDAVAGADL
                     ILLEANHDVDMLKAGSYPYPLKMRILSSRGHLCNEDAGLVLQKLYARGVRNAILGHLS
                     QENNTPELALVTVQSVLESAGLLESVFVTVADRFQPCGMFEI"
     gene            1231..1710
                     /gene="rlmH"
                     /locus_tag="PLR57_05010"
     CDS             1231..1710
                     /gene="rlmH"
                     /locus_tag="PLR57_05010"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQQ40849.1"
                     /translation="MKIKIACVGKLKDSFYREAVAEYAKRLSRFCTLEIAEVADEKAP
                     ESLSPAEERQVMEREGERLLSKVNPGEFAVALCVEGKRMDSPAFAAFLQDAFVNHSPT
                     VTFLIGGSLGLSPAVLARADAKVSLSDMTLPHGLCRVFLTEQIYRAFKINAREPYHK"
     regulatory      1785..1924
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT          455 a          596 c          581 g          529 t
ORIGIN      
        1 tgacgacgat atcgcttgcg cgctctaccg aaaccgtgtc gaacaggtcg atgctttgca
       61 taatcgagtc gagcgcgtgg tacccgtcgc tgcgcttata cagcacgccg agggttaaat
      121 tcagtttcgc gtacgcgcgt tctgttgttc tatccatgca aaacagtata gcaggttttt
      181 acccgctccg cactccattg ctccgcctga atcggcagtt tgagacactg ttactgtcgc
      241 gctgattcgg cttgtgctat ccgagaatta tcttgcctta ccacacacaa cccccgtatc
      301 tccatacgtt actgtccgtt cattcaggct tgtgttatac taggtatcgt taaagaaaag
      361 gagcatcccg actatgatgt tttccccgtt gtgcagcggt tcttccggca acgcgtccta
      421 tctggaagcg ggcggcgttc gtctgctcgt ggacgccggc ttgcccggca agcgcatgga
      481 gcagctgctc gacatgatcg acgtttccgc gcgctcgctc gacgcaattt tggtgaccca
      541 cgagcacagc gaccatgttt cgggcgttgg cgtgctctcg cgcaagtatg atatccccgt
      601 ttacgccgcc gaagcctgct ggcggagcct gcccgcttcg cttggcgcat tcgcgcccag
      661 gaacgtgcgc gtattcgagc cggatcagga cttttactta aagcagctct gcgtgcaccc
      721 ctttaccatt ccgcatgaca gcgcgcaccc cgttggctac accttcgtgc acgacggcag
      781 gaagctcgcc atcatgaccg atatcgggca cgtttccaac acgatgctgg acgccgtcgc
      841 aggagcggat ttgattcttt tggaagccaa ccacgatgtg gacatgctca aggccggaag
      901 ctacccttac ccgctcaaga tgcgcattct ctcctcccgc gggcatctct gcaacgagga
      961 cgcggggctt gtgctgcaaa agctatacgc gcgcggcgtg cgaaacgcca ttttggggca
     1021 tctctcgcag gaaaacaaca cgccggagct cgcgctcgtg accgtgcagt ccgtgctgga
     1081 aagcgcgggt ctgctggaga gcgtgttcgt caccgttgcc gaccgcttcc agccctgcgg
     1141 gatgtttgag atatagggtt ttcttcttct ttcttaaaag aaagaagcaa agaatacttc
     1201 tcttttacaa aaagaacttt ggtttaattg atgaagatta aaattgcgtg cgtgggaaaa
     1261 ctgaaagaca gtttttaccg ggaggccgtg gcggaatacg ccaagcgcct ttctcgtttc
     1321 tgcacgctgg aaattgccga agttgcggac gaaaaagcgc cggaatccct ttcccccgcc
     1381 gaagaacgcc aggtcatgga acgcgaaggc gagcgcctgc tatcaaaagt gaaccccggc
     1441 gaattcgccg tcgccctctg cgtggaaggc aagcgcatgg actcccccgc gttcgcggcg
     1501 tttcttcaag acgcgttcgt gaatcattcg ccaacggtga cgttcctcat cggcggttcg
     1561 ctggggcttt cccccgccgt gctcgcgcgc gcggacgcga aggtttcgct ttccgatatg
     1621 acgctgcccc acggcctctg ccgcgtcttt ttaaccgaac agatctaccg cgcgttcaag
     1681 atcaacgccc gtgagccgta tcataaatga acacaaagtc cagttgttcc agtttttcgg
     1741 cgttgagatt tgacaagtga ttctgtaatc ctatatacta tattttaatt gaatagctga
     1801 atctttcctt attattaaga gaaagaacgg gagaaccaat ctttggggtg aatccggcgt
     1861 ttgccggtag ggcagctttc agcccgaacc cgtcagctaa tctcgtaagc gtttgaaagg
     1921 cttttctttt tgcatgttta aaaagagtcc taatgcgctt gcgatttcgc gggcgctttt
     1981 tgttttttat aggaggtaga atcgatgatt caagtagcag tagccggtgt tggacgcgcc
     2041 ggagcggaag ttataaggga gcttgccgtg agcagtcagt tccgcttagc tgcggctttc
     2101 tgccgcgacg gcagcgagaa ggccggcaaa aaaataacga cacttttacc gacggcagga
     2161 t
//
LOCUS       DAOMGY010000279         2158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_57499_length_2158_cov_2.670946, whole genome shotgun sequence.
ACCESSION   DAOMGY010000279 DAOMGY010000000
VERSION     DAOMGY010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57499_length_2158_cov_2.670946"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PLR57_05015"
     CDS             <1..1178
                     /locus_tag="PLR57_05015"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQQ40850.1"
                     /translation="LNREPKRGGLVDYKTMFQDAQRFIDGLGLRIDAVEKVFRLTIAE
                     QQIIEIVKAISFQSRIIVMDEPTSSLSDNEIDALFEHITALKSKGIGIIYISHRLSEL
                     ARIADRVTVLRDGQSVGSANVADISNDEIVRMMVGREMTHYYTRTYNQATEEALRVEH
                     LTTAHVKDISFSVKKGEILGFAGLVGAGRTEVMKGILGFDPVLSGSVYVGGKRVVIKR
                     PSQAYALKIGYIPENRREESLFSFRSVQYNLTIRVLRSFIRGVSVNGAKEQKITSEYV
                     EKLSIKTASYATKIQNLSGGNQQKVVISRWLAAKPDILIMDEPTRGIDVGAKAEIYEL
                     MNALAAQGMAIIMISSELPEVINISDRVIVMRESRISGMLERSQLSQEAIMQYAVNL"
     gene            1192..>2158
                     /locus_tag="PLR57_05020"
     CDS             1192..>2158
                     /locus_tag="PLR57_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40851.1"
                     /translation="MEAKQMELSSNNGTLKRLGRWFVNYAGQLGFLLILIVVLSFSAP
                     SFLKTNNIINVSRQVSVNMIIACAMTIVLISGGIDLSTGSVMAMSGMVSAYLSFSGFP
                     FVFCALAGILTGVFSGLINGLILANTKLPPFIVTYAMQSILRGVVFVATGAGTIRLEN
                     KAYLDFGGSSIGGIPLPVLYMIAVIVIAWLVLGRSKMGRHIYAIGGNPRAAQFTGINI
                     KRIVVFIYSLCGACAALAGLVLTSRNTSMQPAVGVGSEMDAIAAVVLGGTSMAGGQGA
                     IAGTIIGAFIIGIINNGLNLLGMDSYYQYICKGIVILTAVYMDDVK"
BASE COUNT          466 a          624 c          612 g          456 t
ORIGIN      
        1 tcctcaaccg cgaacccaag cgcggaggcc tcgtggacta caagaccatg tttcaggatg
       61 cgcagcgctt tatcgacggc ctcggactca ggattgacgc cgtcgaaaag gtgtttcgcc
      121 tgacgattgc cgagcagcag atcattgaaa tcgtcaaggc aatctcgttc cagtcgcgca
      181 tcatcgtgat ggacgaacca acctcgtcgc tttcggacaa cgaaatcgac gctttgtttg
      241 aacatattac cgctctcaaa tccaaaggga tcggcattat ctatatttcc caccggctct
      301 cggagctggc gcgcatcgcc gaccgcgtta ccgtgctgcg agacgggcag tccgtaggca
      361 gcgccaacgt tgccgatatt tccaacgacg aaatcgtgcg catgatggtc gggcgcgaaa
      421 tgacccacta ctatacgcgc acctataacc aggccacgga agaggcgctc cgcgtggagc
      481 acctgaccac ggcgcatgtc aaggacatca gcttttcggt caaaaaaggc gaaattttgg
      541 gctttgcggg gctggtcggc gcgggccgga cggaggtcat gaagggcatc ctcggctttg
      601 accccgtgct ctccggctcg gtttatgttg gaggcaagcg cgtcgtaatc aagcggcctt
      661 cgcaggcgta cgcgctgaag atcggttata ttccggagaa ccgtcgggaa gaaagcctgt
      721 tcagcttccg ctccgtgcag tacaacctga ccatccgcgt gctgcgctcg tttatccgcg
      781 gggtaagcgt caacggcgca aaagaacaga agatcacaag cgaatatgtg gagaagctct
      841 ctattaaaac ggcctcctac gcaaccaaga ttcaaaacct ttccggcgga aatcagcaaa
      901 aagtggtcat ctcccgctgg cttgccgcca agccggacat cctgattatg gacgagccga
      961 cgcgcggcat agacgttggc gcgaaggcgg aaatctacga actcatgaac gcgctggccg
     1021 cgcagggaat ggcgattatc atgatctcct cggaactgcc ggaagtcatc aacatcagcg
     1081 accgcgttat cgtcatgcgc gaaagccgca tctccggcat gctggagcga tcgcagcttt
     1141 cgcaggaagc cattatgcaa tacgcggtca atctataacg ggagagagaa aatggaagcg
     1201 aaacaaatgg aactttcttc gaacaacggc acgctcaagc ggctgggacg gtggtttgtc
     1261 aactacgcgg gtcagctggg attcctgctg attctgatcg tggtgctgtc cttcagcgcg
     1321 ccgtcgtttc ttaaaaccaa caacatcatc aacgtttcgc ggcaggtttc cgtgaatatg
     1381 atcatcgcct gcgcaatgac catcgtgctc atttccggcg gcatcgacct ttcaacgggg
     1441 tccgtgatgg ccatgtccgg catggtatcg gcctaccttt cgttcagcgg gttcccgttc
     1501 gtattctgcg cgcttgcggg catcctcacc ggcgtgtttt ccggcctgat caacggactc
     1561 atcctcgcca acacgaagct cccgccgttt atcgtaacct atgccatgca gagcattctg
     1621 cgcggggtcg tgtttgtagc caccggcgcg ggaaccatcc ggctggaaaa caaagcgtat
     1681 ctggactttg gcggcagctc catcggcggc attcccctgc cggtgctcta tatgatcgcc
     1741 gttatcgtga ttgcctggct ggtgctcggc cgctccaaga tgggacggca tatctatgca
     1801 atcggcggca acccgagagc ggcgcagttt acgggcatca atatcaagcg catcgtcgtt
     1861 ttcatctact cgctctgcgg cgcctgcgcg gcgctggcgg gactggtgct cacaagccgc
     1921 aacacctcca tgcagcccgc ggtcggcgtt ggcagcgaga tggacgccat cgcggcggtc
     1981 gtactgggcg gcacctccat ggcgggcggg cagggcgcga ttgccggaac catcatcggc
     2041 gcgtttatca ttggcatcat caacaacggc ctgaacctgc tcggcatgga cagctattat
     2101 caatacatct gcaagggcat cgtcattctg actgcggtct atatggacga tgttaaga
//
LOCUS       DAOMGY010000280         2142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_58103_length_2142_cov_3.741255, whole genome shotgun sequence.
ACCESSION   DAOMGY010000280 DAOMGY010000000
VERSION     DAOMGY010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58103_length_2142_cov_3.741255"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLR57_05025"
     CDS             <1..434
                     /locus_tag="PLR57_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HQQ40852.1"
                     /translation="KDAQAYLSRYRMPSEIRKIVNEHHGTTLVAYFYYKAKKMQEQDG
                     EPVIERLFRYQGGLPSSKESAIVMLADSCEAAVRSMTDPSREEIAEMVRKIVQGKMDD
                     GQLRQSPLTLEEVEKIEKNFLVTFIGLMHERIRYPGTELNP"
     gene            431..874
                     /gene="ybeY"
                     /locus_tag="PLR57_05030"
     CDS             431..874
                     /gene="ybeY"
                     /locus_tag="PLR57_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQQ40853.1"
                     /translation="MIDLQTELEQVPQSALELIRSAVSASLANGGVRGDVCVLITDAE
                     EIQSLNRTYRKIDRVTDVLTFPAWEGEAILCPPDEYLGDIAICYERAVEQAEEYNHSL
                     ERELAFLAVHGSLHLMGYDHMTSEDEAAMFEKQEEVLVSLGLTRE"
     gene            944..1357
                     /gene="cdd"
                     /locus_tag="PLR57_05035"
     CDS             944..1357
                     /gene="cdd"
                     /locus_tag="PLR57_05035"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008431620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HQQ40854.1"
                     /translation="MAKLERAEREKMLDLAYEAMKQAYAPYSHFQVGACLKGANGTYY
                     LGCNIENASYTPTNCAERTAVFKAVSEGEREFTAIAIVCSGKSPAAPCGVCRQVLREF
                     CKEDMPVVFANADRVVIEATLGDLLPHSFGLEDLS"
     gene            1354..>2142
                     /gene="era"
                     /locus_tag="PLR57_05040"
     CDS             1354..>2142
                     /gene="era"
                     /locus_tag="PLR57_05040"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQQ40855.1"
                     /translation="MKEYRSGFVSIVGCPNVGKSTLLNKIIGQKIAIVTDRAQTTRNK
                     ITGVLSRPGYQIVFLDTPGVTNPKNKLGEYMQKVTWDAMNEVEAILFMADAEQGVRER
                     DAALLDRLSSAKAPVIAFVNKCDVCSKGQIDEAVAALEQRSFIKAVLHGSANTGEGLE
                     QLETELLACMGVGPQYFPEDMVTDQPERIICAELIREKALLLLREEVPHGVGVAVDKM
                     ERREDRDMMDVYATIYCERESHKGIIIGKGGSMLKKIGQEARKDI"
BASE COUNT          519 a          574 c          654 g          395 t
ORIGIN      
        1 tcaaggacgc gcaggcgtat ctttcccgct accgcatgcc gtccgaaatc cgcaaaattg
       61 tcaacgaaca ccacggcacg acgctggttg cgtatttcta ttacaaagca aagaagatgc
      121 aggagcagga cggcgaaccc gtgatcgagc ggctgttccg ctatcagggc ggactgccaa
      181 gctccaagga aagcgccatc gtgatgctgg cggacagctg cgaagcggcg gtgcgttcca
      241 tgaccgaccc ttcgcgcgag gagattgccg agatggtgcg aaagatcgtg cagggcaaga
      301 tggacgacgg ccagctgcgc cagagcccgc ttacgctgga ggaagtggag aagattgaaa
      361 agaacttcct cgtcacgttt atcggcctga tgcacgagcg cattcgctac ccgggaacgg
      421 agctgaaccc atgatcgatc tgcaaaccga actggagcag gtgccgcagt ccgcgctgga
      481 gttgattcgc agcgcggttt ccgcctcgct ggcaaacggc ggggtgcggg gcgacgtctg
      541 cgttttgatt acggacgcgg aagaaattca gtccctcaac cgcacgtacc gcaaaatcga
      601 ccgcgttacg gacgtactga cgtttcccgc gtgggaaggg gaggcaatcc tctgcccgcc
      661 ggatgaatac cttggcgata tcgcaatctg ctatgagcgc gcggtggaac aggcggagga
      721 gtacaaccac tcccttgagc gtgaactcgc gtttctggcg gtgcacggtt cgctccacct
      781 gatggggtac gaccacatga cgagcgagga cgaagccgcc atgttcgaaa aacaggagga
      841 ggtgctcgtt tctcttggct taacgcggga gtaaaagagc cttcggctac gtgaaacaac
      901 agaaatatca aacatacgat tacagaacgg agtgaaacag tccatggcaa aactagaacg
      961 ggcagaacgc gaaaagatgc tcgaccttgc atatgaagcg atgaagcagg cgtacgcgcc
     1021 gtattcgcat tttcaggtag gagcctgctt aaaaggcgca aacggaacct attatctcgg
     1081 gtgcaatatt gaaaacgcat cctacacgcc gaccaactgc gcagaacgta cggcagtatt
     1141 taaggcggtt tccgaaggcg agcgcgagtt tacggcaatc gccatcgtct gctccggcaa
     1201 gagccctgcc gcgccctgcg gcgtttgccg tcaggtgctg agggaatttt gcaaggaaga
     1261 tatgcccgtg gtgtttgcaa acgccgaccg cgtggttatt gaagccacgc tgggcgatct
     1321 tttgccgcat tcgttcgggc ttgaggattt atcatgaagg aataccgctc gggctttgtc
     1381 tcgatcgtcg gctgcccgaa cgtcggcaag tccacgctgc tcaacaaaat catcggccag
     1441 aagattgcaa tcgtgaccga ccgcgcgcag accacgcgca ataagatcac cggcgtgctc
     1501 tcacgaccgg gctatcagat tgtgtttctg gatacgccgg gggttacaaa ccccaagaac
     1561 aagctcggcg aatacatgca gaaagtcacc tgggacgcga tgaacgaggt ggaagccatc
     1621 ctttttatgg cggacgccga gcagggcgtg cgcgaacgcg acgcggctct gctagatagg
     1681 ctttccagcg ccaaagcgcc ggtgattgcg ttcgtcaaca aatgcgacgt ctgcagtaaa
     1741 ggccagatcg atgaagcggt tgcagcgctc gaacagagaa gctttatcaa ggctgttctg
     1801 catggttccg cgaacacggg tgaaggcctg gaacagctcg aaacggaact gctcgcctgc
     1861 atgggcgtcg gcccgcagta ttttccggaa gacatggtca ccgaccagcc ggagcgcatc
     1921 atctgcgcgg agttgatccg cgaaaaggcg ctgctgctgc tcagggaaga agtgccgcac
     1981 ggcgtaggcg tcgcggtgga taaaatggag cggcgcgaag accgcgacat gatggatgtt
     2041 tacgcaacca tctactgtga gcgggaatcc cacaagggca tcatcatcgg caagggcggt
     2101 tccatgctca agaagatcgg gcaggaagcg cgaaaagaca tc
//
LOCUS       DAOMGY010000281         2128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_58683_length_2128_cov_3.897733, whole genome shotgun sequence.
ACCESSION   DAOMGY010000281 DAOMGY010000000
VERSION     DAOMGY010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58683_length_2128_cov_3.897733"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLR57_05045"
     CDS             <1..395
                     /locus_tag="PLR57_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HQQ40856.1"
                     /translation="LAVEGGFVTRADAAAEAERFWSGQIALIKVWYLEQKREDDLIIS
                     ASPEFLLMPVCRELGVTLIASQVDPRTGRYDGKNCHDEEKVRRMKEAYPEVEIDRFYS
                     DSRADTPLAKLAKQAFLVKGNALSLFPR"
     gene            625..1251
                     /locus_tag="PLR57_05050"
     CDS             625..1251
                     /locus_tag="PLR57_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQQ40857.1"
                     /translation="MNVVAINGSPRKDGNTAAALRLMADELEKQGIGVEIIQIGHLPI
                     HGCIACGHCQTSEGNWCVFTDDIVNEVSAKMRAADGLILASPTYFAGITGSLKSFLDR
                     VFMSGWGKFRLKVATSVAAVRRAGGVDVVHQLNDFLELAETVIPPSQYWTLAFGMEKG
                     EVLQDGEGTQTLRKNARAMAWLMKLIEAGKGKVPLPEDEPREITNFIR"
     gene            complement(1330..2031)
                     /locus_tag="PLR57_05055"
     CDS             complement(1330..2031)
                     /locus_tag="PLR57_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40858.1"
                     /translation="MLNVQNINVYYGNIHAIKDVSFSVETGEIVSLIGANGAGKSTIL
                     KTVSGLLRTKTGDVSFEGKSIKSVAPHKIVSRGIAHVPEGRRVFLQMSVEENLEMGAF
                     TAQRSTIEGSLENVYERFPRLKERHRQVAGTLSGGEQQMLAMGRGLMSRPKLMMLDEP
                     SMGLAPILVEQIFEIIQELNKTGTTILLVEQNAQMALSIATRGYVLETGKIVLAASAD
                     ELLVNDDVRKAYLGG"
BASE COUNT          462 a          571 c          639 g          456 t
ORIGIN      
        1 atctggcggt tgaaggagga tttgtcactc gtgcggatgc ggcggcggaa gccgagcggt
       61 tctggagcgg tcagattgcg ctgatcaaag tctggtatct cgagcagaag cgggaggacg
      121 atctgatcat ctccgcttcg cctgagttct tactgatgcc ggtctgccgc gaacttggcg
      181 taacgctcat tgcgtcgcag gttgatccca ggacgggcag gtacgacggc aaaaactgcc
      241 acgacgaaga gaaggtgcgc cgcatgaagg aagcgtaccc tgaggtcgaa atcgaccgct
      301 tttactcgga cagccgtgcg gatacgccgc tggcaaagct cgcaaagcag gcgtttctgg
      361 tcaaagggaa cgccctctcg ctgtttccgc gctgaacaac gttcggttga caatcagccg
      421 agacaacacg aaatgcgacc caatcagctt cttcgggggc tgatttttga ttgatcgggg
      481 attttacaaa tcaatcatcc ggattgcact gcgtttactg ctgaaaacaa tgaaatactg
      541 ttgaagtacg cgcaatatct gatatgatac tctcatcaac agatactgga cgcagactgg
      601 cagaatcgaa aggagaaagt tcgtatgaac gtggttgcga ttaacggcag tcccagaaag
      661 gacggcaata ccgcggcggc gctgcggctg atggcggacg aactggagaa gcagggcatc
      721 ggcgtggaga tcatacaaat cgggcattta cccatccacg gctgcatcgc ctgcgggcat
      781 tgccagacgt ccgaggggaa ctggtgcgtg ttcacggatg atattgtcaa cgaagtctcc
      841 gcaaagatgc gcgcggcaga cgggctgatc ttagcctcgc caacgtattt tgcgggaata
      901 accggcagct tgaaatcgtt tctcgaccgg gtgttcatgt ccggctgggg aaagttccgc
      961 ctgaaggtgg caacatccgt ggcggcggtg cgccgcgcgg gcggggtgga cgtggtgcac
     1021 cagctcaacg actttctcga actcgcggaa acggtgatac ccccttcgca gtactggacg
     1081 ctggcgttcg gcatggaaaa gggcgaagtg ctgcaggacg gcgaaggcac gcagacgctg
     1141 cgtaagaacg cacgcgcgat ggcatggcta atgaagctca tagaagcggg caaaggcaaa
     1201 gtcccccttc cggaggacga accgagagag ataaccaact ttatccgctg aacgaattcg
     1261 aacaaacaaa aaccgcgctt tcgcgcggtt ttttcgcgca gaagcgcggt tactttgata
     1321 ttcgaacgat cagccgccga ggtaggcttt ccgcacgtcg tcgttgacca gcagttcgtc
     1381 cgccgacgcg gcgagcacga ttttgccggt ttccagcacg tacccgcgcg tggcgataga
     1441 gagcgccatc tgcgcgtttt gctcgacgag cagaatcgtc gtaccggttt tgttgagctc
     1501 ctgaataatc tcgaaaatct gctccacgag gatcggtgca agccccatgg aaggttcgtc
     1561 cagcatcatg agcttggggc gggacatcag tcctctgccc atggcgagca tctgctgttc
     1621 gccgccggag agcgtgcctg ccacctgcct gtggcgttcc ttgaggcgcg gaaagcgctc
     1681 gtagacgttt tccagcgagc cttcgatggt ggagcgctgc gcggtgaacg cgcccatttc
     1741 gaggttttct tccacgctca tctgcagaaa gacccttcgg ccttccggca cgtgcgcaat
     1801 gccgcgggag acgattttat ggggagctac ggacttgatg ctcttgcctt caaacgaaac
     1861 gtctccggtc tttgtgcgca gcaggcccga caccgttttg aggatggtgg acttgcccgc
     1921 gccgtttgcg ccgattaacg agacgatttc gcccgtttca acggagaagg atacgtcctt
     1981 gatggcatgg atattgccgt aatacacatt gatgttctga acgttcagca tcaggctttc
     2041 gcctcctttc cgcggccaag atacgcttca atgaccaccg gattgcactt gacctgctcc
     2101 ggcgtgcctt ttgcgatgat cttgccgt
//
LOCUS       DAOMGY010000282         2127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_58728_length_2127_cov_3.124517, whole genome shotgun sequence.
ACCESSION   DAOMGY010000282 DAOMGY010000000
VERSION     DAOMGY010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58728_length_2127_cov_3.124517"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLR57_05060"
     CDS             complement(<1..448)
                     /locus_tag="PLR57_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40859.1"
                     /translation="MKRTILSFALAVLLISAVGCQQQSAPVTVVDVTPTPSQAPANVP
                     DVPIQTATEAPVVTEVPTVTEAPSDAFSLSSTGIVDGAIADDFGARASQTKAGIPNRS
                     FELAFANVPEGTACIALTMIDPDGGDWVHWLVANAPVEGLPENASVD"
     gene            complement(576..1877)
                     /locus_tag="PLR57_05065"
     CDS             complement(576..1877)
                     /locus_tag="PLR57_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40860.1"
                     /translation="MKTTLRALTILLLLACTLVFAACGIAPQHEKAIENAIIAYSDYY
                     IEVRDLGEQVDAQAKAFDQDAASFEYTITALVPDYTSLTQETLPFTPPGVDFNEPNSA
                     AYRQNALYALRQAAETYALEHEFTSYAEVPLTVVVEQSGSDWTATISSTSKKSIQTTA
                     EYLLSNLLDTYDSFQQNSRLAFIAESKTSLLQDVFGGSGYANAATVESVAPLGGGLYE
                     LSLSFPDPALVYSALAEDYFASFNQPFFGEEMTVTLSADDLSGINTTAMQTKSASVTV
                     AYDENTVTCSLTDASALSALIDPAKNQAEQTVSARVNADWRVPAAEPPASGKVLEGES
                     RGNEINFITSADLGAYYYVRFYLLSGDDVSEEGTLAAGIFITGGKKATIRLPSGYYRV
                     TCLVGNTWYGLDYLFGTDSKTYNGSNAVQSRSGYINTISFG"
BASE COUNT          444 a          570 c          628 g          485 t
ORIGIN      
        1 gatcgacgga agcgttctcc ggtaagcctt cgacgggcgc gttggcgacc agccagtgca
       61 cccagtctcc gccgtcggga tcgatcatcg tcagcgcgat gcaggccgtt ccttccggta
      121 cgttggcaaa cgccagctcg aacgagcggt tgggaatgcc cgctttcgtc tgggaagcgc
      181 gcgcaccgaa atcgtccgca attgcgccgt ccacgatgcc ggtgctggaa agcgagaatg
      241 cgtcgctcgg tgcttccgtt acagttggca cttccgttac aactggcgct tccgtggcgg
      301 tttggatggg tacgtccggc acgttcgcgg gcgcctgcga cggggttggg gtaacgtcca
      361 caaccgtaac gggcgcggac tgctgctgac agccaacggc tgatataagc aaaacggcga
      421 gcgcgaagct aagtatggtt cgtttcattc ggttcgctcc tttcagtatt cggtttcaaa
      481 ataactgcaa attcagtgta cccaaaagta cataaaaaat caacggcggg gaaaccgccg
      541 ttaagatatt agaaattcaa ttcgctttgc ggcgatcagc caaacgaaat ggtgttgata
      601 taccccgaac gggactgtac ggcgttggaa ccgttatagg ttttgctgtc cgtgccaaag
      661 aggtagtcca gcccgtacca ggtgttgccg accagacagg tcactctgta gtagccgctg
      721 ggcaggcgga ttgtcgcctt tttgccgccg gttataaaga tgcctgccgc gagcgttccc
      781 tcctcggaaa cgtcgtcgcc gctcagcaga tagaagcgaa cgtaatagta tgcgccgaga
      841 tccgcgctgg tgataaagtt tatttcgttg ccgcgacttt cgccttccag cacttttccg
      901 cttgcgggcg gttccgcggc gggtacccgc cagtccgcgt tgacgcgcgc ggaaaccgtc
      961 tgttccgcct gattttttgc ggggtcaatc agcgcggaaa gagcgcttgc gtcagtgagc
     1021 gagcaggtta ccgtgttctc gtcgtatgca actgttacgg acgcgctctt ggtctgcatc
     1081 gcggttgtat tgatgccgga gagatcgtcc gcagagagcg taacggtcat ctcttcgcca
     1141 aagaacggct ggttaaagga agcgaaataa tcctccgcca gcgcgctgta aacgagcgcg
     1201 gggtcgggga aggagaggga cagttcatat aagccgccgc cgagcggggc aacgctttcc
     1261 acagtcgccg cgttagcgta accgctgccg ccgaaaacgt cctgcagcag gctagttttc
     1321 gattcagcga taaaggcaag gcggctgttt tgctggaatg agtcgtaggt atccagaaga
     1381 ttgctcagca ggtactccgc ggtggtttga atgctttttt tactcgtgct ggatattgtt
     1441 gccgtccagt cgctgccgga ttgctctaca accacggtga gcggcacttc cgcataggat
     1501 gtgaactcat gctcaagcgc gtacgtttcc gccgcctgcc gtagcgcata gagcgcgttc
     1561 tggcggtatg ccgctgagtt cggctcgtta aaatcgacac ccggcggggt aaagggcagc
     1621 gtttcctgcg taaggctcgt gtagtcgggg acgagcgcgg taatcgtgta ttcaaacgac
     1681 gcggcgtctt gatcgaacgc ctttgcctgc gcatcgacct gttcgccgag gtcgcgcacc
     1741 tcgatataat agtccgagta agcgatgatt gcgttttcaa tcgccttttc gtgctgcggg
     1801 gcaataccgc acgcggcaaa aaccagcgtg caggctagca ggagcaaaat tgtaagtgcg
     1861 cggagcgtcg ttttcatgcg gcagttcctt cccgtccatc gggcaaatca gttttgcatt
     1921 acataaaaat gcatttattt acaagtaaac tgtaacatga cgttgcttat gcgtcaatca
     1981 atgcgaataa atattcttcg tatcgtccgg ctccctctga cgagggagct gccgccgaag
     2041 tcggctgagg gagagagaaa ataactcttt cgctccctcc ggcgcttcgc gccacctcac
     2101 tatttcgagg gaggcaagag aaggcac
//
LOCUS       DAOMGY010000283         2125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_58811_length_2125_cov_2.854589, whole genome shotgun sequence.
ACCESSION   DAOMGY010000283 DAOMGY010000000
VERSION     DAOMGY010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58811_length_2125_cov_2.854589"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PLR57_05070"
     CDS             <1..882
                     /locus_tag="PLR57_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HQQ40861.1"
                     /translation="YEKPRAPRGKKKRSKFGKFMHALGLYLAQLPAKTLVIFGGSFAM
                     ILVAVILLAVLLPNNARTERPVDGQLALSDVTVTPSLAPTNTPAPTEEMTPEPTIPAL
                     TEPISKSGTVSDLMPAIQERLVELGYMEMPTDGYTTKFGPITKTAIRLFQMKNFTDYN
                     SWDGIIGNSTYSLLMSSDAKAYYLTRGDGDDRTKVITKLVEDVTDLQKRLIELGYLAA
                     GSASGLYGNSTALAVQTFQEYHGLQADGKAGQETLKLIYSADAMDATTGKANNKSKMS
                     PTPSTSPSTEVSTTPAP"
     gene            complement(959..>2125)
                     /locus_tag="PLR57_05075"
     CDS             complement(959..>2125)
                     /locus_tag="PLR57_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQQ40862.1"
                     /translation="IAVLAAVIWAIAGKDFNFVLNIFVTVLVIACPCALGLATPTAIM
                     VGTGKGAELGVLMKGGEALETTHKIDTVVLDKTGTITQGKPELTDIRLYASGTENELL
                     ALSASAERGSEHPVARAIVEAAEARRLTLASPESFRAVAGRGIDAVVSGRRVLAGNEK
                     LMLENGISLSAASNDALALSGAGKTLMYVAVDGSLAALLAAADAVKPTSRRAVERLNA
                     LGLEVYMLTGDNAATAAAVAGSVGITRVISEVLPEGKAGEVQKLQNLGKRVAMVGDGI
                     NDAPALAQADVGMAIGTGTDVAVESADVVLMQGDLNAVATAIALSRATIRNIRQNLFW
                     AFAYNVVGIPFAAGLFFALGGPLLTPVFAGAAMALSSVSVVTNALRLRRFRVKD"
BASE COUNT          456 a          706 c          589 g          374 t
ORIGIN      
        1 tacgaaaaac cccgcgcgcc cagaggaaag aaaaaacgca gtaaattcgg caagttcatg
       61 cacgcgctgg ggctttacct ggcacagttg cccgcaaaaa cgctggtaat ctttggcgga
      121 tcgtttgcca tgattctggt ggcggtgatc ctgctggcgg tactgctgcc gaacaacgcg
      181 cgcaccgaac gccccgtgga cggccagctc gcgctttcgg atgttacggt taccccgtcg
      241 ctggcgccga ccaacacccc cgcgccaacc gaagaaatga ccccggaacc gaccattccg
      301 gcgctgacgg agcccatctc aaaatccggc acggtcagcg acctcatgcc cgccattcag
      361 gagcggctgg ttgaactggg ctatatggaa atgcctaccg acggctatac aacaaagttc
      421 ggccccatca cgaagaccgc catccgcctc ttccagatga agaatttcac cgattacaac
      481 agctgggacg gcattatcgg caacagcacg tattcgctgc tcatgagcag cgacgcaaaa
      541 gcttattacc tgacgcgcgg cgacggtgac gaccgcacca aggtcatcac gaagctcgtc
      601 gaagatgtta ccgacctgca gaaacgcctc atcgaactcg gttatctggc ggcaggaagc
      661 gcatccggcc tctatggcaa ctccacggcg cttgcggtgc aaaccttcca ggaatatcac
      721 ggcctgcagg cagacggcaa ggccgggcag gaaacgctga agctgatcta cagcgcggac
      781 gcaatggacg caacgaccgg caaggcgaac aacaagtcca agatgtcgcc cacgccgagc
      841 acctcgccct ccaccgaagt ttcaaccacg cctgctccgt aacaacaatt tctcaatcaa
      901 tagagataca caaaaacacc gtaccggttc tccggtacgg tgtttttgtg ttatgctgtc
      961 agtctttcac gcggaacctt ctgaggcgca gcgcgttggt aacaacggaa acggagctca
     1021 gcgccatggc cgcccctgcg aacaccggag tcagcagcgg gccgccgagc gcaaagaaca
     1081 atcccgccgc aaacggaatg ccgaccacgt tgtaggcaaa cgcccagaag agattctggc
     1141 ggatattccg aatcgtcgcg cgggagagcg caatcgcggt cgccacggcg ttgagatcgc
     1201 cctgcatgag caccacgtcc gcggattcta cagcaacgtc cgtgcccgta ccgatcgcca
     1261 tgccgacgtc cgcctgcgcc agcgcgggcg cgtcgttgat gccgtcgccc accatcgcca
     1321 cgcgcttgcc gaggttctgc agtttctgca cttccccggc tttgccttcg ggcagcactt
     1381 ccgaaatcac gcgggtgatt ccaacgctcc cggcgaccgc agccgcggtg gctgcgttgt
     1441 ccccggtgag catgtaaacc tccagcccaa gcgcgttgag ccgttccacg gcgcgcctgc
     1501 tcgtaggctt gaccgcgtcc gccgctgcca aaagcgcggc aagagaaccg tccacggcaa
     1561 catacatcag cgtcttaccc gcgccggaaa gcgcgagcgc gtcgttcgaa gcggctgaaa
     1621 ggctgattcc gttctccagc atcagtttct cgttgcccgc aagcacacgc ctgccggaaa
     1681 ccactgcgtc gatgccgcgc cccgccaccg cgcggaagct ttccggcgat gccaacgtga
     1741 gccttctcgc ctccgccgct tccacaatag cgcgcgcaac aggatgctcc gagccgcgct
     1801 ccgccgatgc gctgagcgca agcagttcgt tctccgtgcc ggaagcgtaa agccggatat
     1861 cggtcagttc gggtttgccc tgcgtaatcg tgcctgtttt gtcgagcacg acggtatcga
     1921 ttttatgcgt cgtctccaac gcttcgccgc ccttcattag tacgcccagt tccgcgccct
     1981 ttccggtgcc gaccatgatg gccgtagggg ttgcgagccc cagcgcgcac gggcaggcga
     2041 tgaccagcac ggtgacgaag atattgagca cgaagttgaa gtcctttccg gcaattgccc
     2101 aaatcaccgc cgcaagcacg gcaat
//
LOCUS       DAOMGY010000284         2121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_58980_length_2121_cov_2.622943, whole genome shotgun sequence.
ACCESSION   DAOMGY010000284 DAOMGY010000000
VERSION     DAOMGY010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58980_length_2121_cov_2.622943"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..>2121)
                     /locus_tag="PLR57_05080"
     CDS             complement(300..>2121)
                     /locus_tag="PLR57_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40863.1"
                     /translation="EANRMRKETEKKKPTREERKASLQKSKRTLRHILMEAKPIRWWL
                     ALAGFISVISVGMVLAGPELLGKQTDVLYQFWANGTAIDMPSFIRQCAVLGGVYLIAG
                     VCSLATMFIMNNVVSKLFTCELRIRMSDKIRRLAVKFVDDTPKGEVISRMTNDVSVLG
                     NTVHTVLDLTISGIIKLVGITVIVFTIQPLMALSVVVLVPLSLTIASLISQRSEKHFS
                     DGREFIGKIYALNEEDFAGFATIKAFHLEDRQKRAHETLVDTAKGHLQSGYYLAGIVQ
                     PIVALTNNLAYIAICLLGGALAINGTINVGEVVAFVLYAKLFAGPLESISGGLSTMQH
                     TLASAGRVYDLLDREEMVQPVERPLPQGGGHIRFEHVNFAYDPAKPLIQDLNIDVHPG
                     QKVAIVGPTGGGKTTIVNLLMRFYDVDSGCITIDGVDTKSMDRADLRSMFSMVLQDTW
                     LFSGSIYENIAYGKAGATREEVLEAARRAHIDFFVETLPEGYETQINEESTNVSSGQK
                     QLLTIARAYLANRNILILDEATSNVDTRTEILIQQTMDELMRARTSFVIAHRLSTIVN
                     ADTILVVNDGQIVEQGTHQELLAKNGFYAEIYNSQYALLN"
BASE COUNT          475 a          579 c          551 g          516 t
ORIGIN      
        1 atagtcgcgg tcaaactcga acttctcggc aaggtaggcg tacgcgagat cgcgcacggc
       61 ctttttgctg gggagctgtt taagtttgcc ttcttcatcc aaaaaccgtt caattccggg
      121 aagcataggg ttctcctttt tcctttctta aaaagaaagg aaacgaaaga atttacttct
      181 tcttttctta aaagaaaaga agcaaaagaa tgttttcttt gattctttct ttttcaaaag
      241 aaagaatgtg caatcgaacg ttttctttga ttctttcttt ttcaaaagaa agaatgctct
      301 taattcaaaa gcgcgtactg gctgttgtag atctccgcgt agaagccgtt cttagcaagc
      361 agttcctggt gtgttccctg ctccacgatc tgcccgtcgt tgacaacgag gatcgtatcc
      421 gcgttaacga tggtcgagag ccggtgcgca atgacaaagc ttgtccgcgc gcgcatgagc
      481 tcgtccatgg tctgctgaat gagaatctcc gtgcgggtgt ccacattgct ggtcgcttcg
      541 tcgagaatga ggatgttcct gttggctaag tatgctcgtg caatggtcaa aagctgcttt
      601 tgcccgctgc tgacattggt cgattcctcg ttgatctgcg tttcgtagcc ttccggcagc
      661 gtctcaacaa agaagtcgat atgtgcgcgg cgtgccgctt ccagcacttc ctcgcgggtt
      721 gcacccgctt ttccataggc tatattctca taaatcgagc cggaaaacaa ccatgtatcc
      781 tgtaaaacca ttgaaaacat tgaccgcaaa tccgcgcggt ccatcgactt tgtgtccacg
      841 ccgtcaatcg taatacaccc ggaatccacg tcgtaaaacc gcatcaacag gttgacgata
      901 gtcgtctttc ccccgccggt cggccctacg atggcgactt tctggccggg gtgcacgtcg
      961 atattcaaat cctgaatcaa cggctttgcc ggatcgtagg caaagtttac atgctcaaag
     1021 cggatgtgcc cgccgccctg cggaagcgga cgctcgacag gctgaaccat ctcttcccgg
     1081 tcgagcaggt cgtagacgcg ccccgcggaa gccagcgtat gctgcatggt ggaaagtccg
     1141 ccggatatgc tctccagagg gcctgcaaag agctttgcgt aaagtacgaa cgcgaccact
     1201 tcgccaacgt tgatcgtgcc gttaatcgcc agcgcgccgc cgagcaggca gattgcgata
     1261 tacgccagat tgttggtcag cgccacgatg ggctggacga tgccggcaag gtagtagccg
     1321 gactggagat gtcccttggc cgtgtctacg agagtttcgt gcgcgcgctt ctggcggtct
     1381 tccagatgga acgctttgat cgttgcaaac cccgcaaaat cctcttcgtt gagtgcgtaa
     1441 atcttgccga taaactcccg cccgtcggaa aagtgcttct cgctcctctg cgaaatcaag
     1501 gaagcgatcg tcagcgaaag cggaacgagc acgacaacgc tcagcgccat cagcggctga
     1561 atggtaaaca cgatcaccgt aataccgacg agcttgatga tacctgagat ggtcaaatcc
     1621 aacaccgtat gcaccgtgtt gccgagaacg gaaacgtcgt tggtcatgcg cgagatgact
     1681 tcgcccttgg gcgtatcgtc cacaaacttg accgcgagcc gccgtatctt gtcgctcatg
     1741 cggatgcgca gttcgcaggt aaagagcttg gaaacgacgt tgttcataat gaacatggtc
     1801 gccagactgc aaacgcccgc aatcaggtac acgccgccga gcacggcgca ctgcctgata
     1861 aaagagggca tatcgattgc tgtgccattt gcccagaact gatatagaac atccgtctgc
     1921 ttgcccagca gttccggccc ggccagcacc atgccgacgg agatgacgct gataaagccc
     1981 gcgagcgcaa gccaccagcg gatgggtttt gcttccatca ggatgtgcct gagcgtgcgc
     2041 ttgctcttct gcaaggacgc tttccgttct tcgcgggtcg gttttttctt ttcggtttct
     2101 ttacgcatcc tgttcgcctc c
//
LOCUS       DAOMGY010000285         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_59621_length_2105_cov_3.522439, whole genome shotgun sequence.
ACCESSION   DAOMGY010000285 DAOMGY010000000
VERSION     DAOMGY010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59621_length_2105_cov_3.522439"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLR57_05085"
     CDS             complement(<1..233)
                     /locus_tag="PLR57_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40864.1"
                     /translation="MIEYGTVFSNNTSFLNDNATPDSGFQTQTGFSAATPSGAANTTP
                     APVETAPAAVETTPNPTPDASTLVAESVSLEGTP"
     gene            complement(230..835)
                     /locus_tag="PLR57_05090"
     CDS             complement(230..835)
                     /locus_tag="PLR57_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQQ40865.1"
                     /translation="MNMQRCPSGHYFDKSKFDQCPYCNPATSSDNRTIPLESTMAGTS
                     DPGFGGGWNNNTSDANDIGVTVPLQSGSEVTVALTPKDETGKTFDPVVGWFVCVDGPD
                     KGLDYRIRSGNNSIGRGTGAQIRILNDSSISKENMSHVAFDSRSRRFYFEAGEGRNLI
                     RVNDELLLPHQSRELRAFDRVLLGQTTLAFIPLCGEEFSWE"
     gene            complement(949..1773)
                     /locus_tag="PLR57_05095"
     CDS             complement(949..1773)
                     /locus_tag="PLR57_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQQ40866.1"
                     /translation="MLTLIGAAILLFTPLISMPLVGSYSYHDIAFNPALSGSALSMAM
                     DYSSEAAFTLIALKAIGLTFYAAILLSLVALFQKIDKLTQVFRYLSLSFGGILLLALL
                     YLKSQTSQSLLTDSLVNIRFGVFLGIACLIAAIVLSYRRDVLTRELYRLRSGSQGGAV
                     MADPPLEKRAGALVCTRGEFSGARFDLSRSPTITIGRDARSCQIVFPKDMQRISRKHS
                     LVRFSSSENCYYVTDVSQNGTYLAGGVRLPAGQPCKLARGTTITFDSSGANAFYLE"
BASE COUNT          484 a          559 c          578 g          484 t
ORIGIN      
        1 ggcgttcctt cgagacttac ggattccgca acaagcgtgc ttgcgtccgg cgtggggttt
       61 ggcgttgttt caacagcggc aggcgccgtt tcaacaggtg caggcgtcgt attagctgcg
      121 cccgacggcg ttgccgcgct gaagccggtt tgcgtttgaa acccgctgtc cggcgtcgcg
      181 ttgtcgttta aaaacgacgt attgtttgaa aatacagttc cgtactcgat cattcccaag
      241 aaaactcctc tccgcaaagc ggaataaacg caagtgtggt ctgtcccagc agcacgcggt
      301 caaacgcgcg cagttcgcgg gactggtgcg gcagcagcag ttcgtcgttg acgcggatta
      361 ggtttctgcc ttcacccgct tcaaagtaga accggcgcga ccggctgtcg aacgccacat
      421 gggacatgtt ctccttggaa atcgagctgt cgttcagaat gcggatctgc gcgcccgttc
      481 cgcggccaat cgagttgttg ccgctgcgga tgcggtagtc caggccttta tccggcccgt
      541 ccacgcagac aaaccagccc accacggggt cgaacgtttt gccggtttca tccttcggcg
      601 tgagcgcaac cgtaacttcg ctgccggact gcagcggaac ggtgacgccg atgtcgtttg
      661 cgtcggacgt gttgttgttc catccgccgc cgaagccggg atccgaagtt cctgccatcg
      721 tgctctccag cgggatcgtc cggttgtccg aactcgtcgc cgggttgcag tatgggcact
      781 ggtcaaactt ggatttgtcg aagtagtgtc cgctgggaca acgctgcatg ttcatgagtt
      841 taccccctga ttcatataga tgtatggatt ctttatcaac aatagaagct ttttaaaaag
      901 cttttattta cgcgtgaaaa agctctcttg tatttgcttg gagagggact actccagata
      961 aaacgcgttt gcgccggagg agtcgaaggt aattgtcgtt ccgcgcgcaa gtttgcaggg
     1021 ctgtcccgcg ggcagacgaa cgcctccggc aagataggtg ccgttttgcg aaacatccgt
     1081 aacgtaatag cagttctcag aggagctgaa ccgtacgaga ctatgctttc tgctgatgcg
     1141 ctgcatatcc tttgggaaca cgatctggca ggaacgcgcg tccctgccga ttgtgatcgt
     1201 cgggctcctc gaaaggtcga accgtgcgcc cgaaaactct ccgcgcgtgc agaccagcgc
     1261 gcccgcgcgc ttttcaagag gtgggtcggc cataacggcg ccgccttgcg aaccggagcg
     1321 cagccggtag agctcgcggg taagaacgtc ccttctataa ctcagcacaa tggcggcaat
     1381 caggcatgct atgccgagaa aaacgccgaa ccggatatta acaaggctgt cggtcagcag
     1441 gctttgggat gtctgtgatt tcagatagag aagcgcaagc agcaggatgc cgccaaacga
     1501 cagagaaaga tagcggaaca cctgcgtcag cttatcgatt ttttgaaaca gcgcaacaag
     1561 ggaaagtaga attgcggcat aaaaagtcag accgatggct ttgagcgcaa tcagcgtaaa
     1621 ggccgcctcc gaggagtaat ccatggccat ggagagcgcg ctgccgctca aagccgggtt
     1681 gaacgcaata tcgtgataac tgtaggaacc gacgagcggc atactgataa gaggggtgaa
     1741 aagaagaatc gcagcgccga tcagggtgag catattgacg caaaattctc tcgccgtcca
     1801 taccgtggag cgaaacccgc ttctgagcgg tcttccgcag aaaacgcaaa cgtccccatc
     1861 gtttgccttt ccgcaagccg gacaatatcg cattgtgttc cccttccaac cattccgttg
     1921 cttgaatata caatacttat aatgtaaccc attttaacac gtgttacaag gttgaatggt
     1981 tgataattta tccatgtaat cactatttct tacaattatt cgatgtaagt ttttcatgct
     2041 ttcctctgcc gcggcgcaaa tccgtttgaa tgatttccgc acctgtaaaa aacgcgaaaa
     2101 accgc
//
LOCUS       DAOMGY010000286         2098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_59942_length_2098_cov_2.940773, whole genome shotgun sequence.
ACCESSION   DAOMGY010000286 DAOMGY010000000
VERSION     DAOMGY010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59942_length_2098_cov_2.940773"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PLR57_05100"
     CDS             complement(<1..1255)
                     /locus_tag="PLR57_05100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ40867.1"
                     /translation="MNPELLCMRCMSSLHEPNGVCPVCGFANAAAQNAPNQLDCGSIL
                     AGTYLVGCVLGQGGFGITYIGWDLNLGIKVAIKEYYPEGCVLRDIHTYSDVRPLPGAR
                     GEAFLSGKEHFVREAQILARFSGEPGVIGVRAFFQENGTAYIVMDYAEGETLKKYVSS
                     RGGKLSAAETLALFRPLVLSLMRVHEARLLHRDISPDNIIRRPDGTLVLLDFGAARQM
                     SMSGEHSNTINVKHGFAPEEQYRTHGEQGPWTDIYALCATIYRLTTGVTPPNALDRLM
                     SESGLVPPNALGADFTSAQQNAILHGLKVRVSERTRSLQQLEKELFGEGFKDRKDDKR
                     SRNRWKQAAILGFSACLIACIALVILLNKDRSHSAESPTTNTQLDSAEATIAPTSEPT
                     VKAALESSPAPAQEQPGTNTLPETFE"
     gene            complement(1361..>2098)
                     /locus_tag="PLR57_05105"
     CDS             complement(1361..>2098)
                     /locus_tag="PLR57_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40868.1"
                     /translation="DLSIADCDEVVLAGAGGKFPVNILSLSNCGNIDAGELAQFSNMS
                     SLSIWSTPVTSPEQIGGLTNLTSLTMGYTGVSNLDFILNLRDLYFLSLSGEEISQLPS
                     LDSLTGLTMLHLNYCSLDNSDMRQIASLTGLTELMLDGCPISDLSFVENLTNLMTLSF
                     DSSQVRDISPLKNLTKLETLWLFYVEVKDISVLQYCKSLKMVWIMQTNITDYSPLAGL
                     PITDIYVDEAYVDQVKTYLPNANVNGW"
BASE COUNT          462 a          573 c          573 g          490 t
ORIGIN      
        1 attcgaacgt ttcgggaagc gtgttcgtgc cgggctgctc ctgcgcggga gcgggcgaag
       61 attccagcgc cgcttttaca gttggttcgc tcgttggcgc aatcgtcgct tccgcgctgt
      121 ccaattgcgt atttgtcgtc ggcgattcgg cggagtgcga tctgtctttg ttgagcagta
      181 taaccagcgc aatacaggca atcaggcagg cggaaaatcc gagtattgct gcctgcttcc
      241 agcggttccg gctcctcttg tcgtctttac ggtctttaaa gccttccccg aacagttctt
      301 tttcaagctg ttggaggctg cgtgtccgtt cggaaacgcg caccttcagc ccgtgcaaaa
      361 ttgcattttg ctgcgcgctg gtaaaatccg cgcccagagc gttcggcggt actaagccgc
      421 tttcgctcat caggcggtcc agggcgttcg gcggagtaac gcccgtagtc aggcggtata
      481 tggttgcgca caacgcatag atatccgtcc agggaccttg ttcgccatgc gtacggtatt
      541 gctcctcggg tgcaaatcca tgctttacgt tgatcgtgtt tgagtgctcg ccggacattg
      601 acatctgccg cgccgccccg aaatccagca gcaccaacgt cccgtccgga cggcgaatga
      661 tattgtcggg gctgatatcc cgatgcaaaa gcctcgcttc gtgcacacgc ataagcgaca
      721 aaacgagcgg gcgaaacagc gcaagcgtct ccgcggccga aagtttgccg ccgcggctgg
      781 agacatactt tttgagcgtt tccccctcgg cgtaatccat tacgatatag gcggtgccgt
      841 tttcctgaaa gaaagcgcgt acgccgatta cgcccggttc accggaaaac cgcgccagga
      901 tctgcgcctc ccgcacgaaa tgttctttgc cgctcaggaa ggcttcgccg cgcgcgccgg
      961 gcagcggcct tacgtcggaa taggtatgaa tgtcccgcag aacacagcct tccggatagt
     1021 attccttaat ggcaaccttg atgccgaggt tgagatccca gccgatatat gtaatgccga
     1081 acccgccctg tccaagcacg cagccgacca gatacgtccc cgcgagaatg ctcccgcagt
     1141 cgagctggtt gggcgcgttt tgcgcggcgg cgttggcaaa cccgcagacc gggcaaacgc
     1201 cgttcggctc atgcaggctt gacatgcagc gcatgcagag cagttccggg ttcatgagcg
     1261 tcctccttac agggttcctt gttctgtaaa gcgcggcagt aacggttgct gcgaacgctg
     1321 tctctgctga ttaacgccat caagcgattg aaagagcggc ttaccagccg ttgacatttg
     1381 cgttgggcag gtacgttttc acctgatcca cgtatgcttc gtcaacgtaa atatcagtaa
     1441 ttggcagtcc tgccagtggg gaataatcgg tgatgtttgt ttgcataatc caaaccatct
     1501 ttaaactctt gcagtactgc agtacgctga tatcttttac ttccacgtaa aagagccaaa
     1561 gggtctcaag tttggtcaag ttcttcagag ggctgatgtc ccgcacctgc gagctgtcaa
     1621 aggagagcgt catcaggttt gtaagatttt caacaaagct cagatcggaa atcggacaac
     1681 cgtcgagcat caactcggta agtccggtaa gagaagcgat ctggcgcatg tcggagttgt
     1741 ccagagaaca gtagttcaga tgcagcatcg tgagtcccgt cagagaatcc agtgagggca
     1801 gctgcgaaat ctcttctccg ctcagcgata agaaatataa gtcccttaag ttcagaataa
     1861 agtcgagatt tgaaacacca gtataaccca ttgtaaggct cgtaaggttc gtcaagccgc
     1921 cgatttgttc tggggaagtc acaggagtac tccaaatact caacgaactc atgttggaga
     1981 actgcgccaa ttctccggcg tcaatgttgc cacagttgct cagcgaaaga atgttaacag
     2041 ggaattttcc ccctgcgccc gcgagcacaa cttcgtcgca gtcggcaatc gagaggtc
//
LOCUS       DAOMGY010000287         2096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_60014_length_2096_cov_3.535032, whole genome shotgun sequence.
ACCESSION   DAOMGY010000287 DAOMGY010000000
VERSION     DAOMGY010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60014_length_2096_cov_3.535032"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLR57_05110"
     CDS             <1..237
                     /locus_tag="PLR57_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQQ40869.1"
                     /translation="DERVLLQGVMDACFVENGAWTILDYKTDRVRPGESAEQAAQRHA
                     LQLSLYALALEALTKLPVKEKLVVMLSYRAVIAV"
     regulatory      333..486
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            576..1205
                     /locus_tag="PLR57_05115"
     CDS             576..1205
                     /locus_tag="PLR57_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HQQ40870.1"
                     /translation="MKQKSLADRLAKLGMLTALSVLLVYAIHFPIFPAASFLEYDMAD
                     VPILIGTFLYGPWWGLALTAVVSLLQWLLVSPQSMWVGAVMHFCATGSYVIAAGLIYG
                     KMRTLKGALIGMAVGSVLQTAMMIPMNLIFTVHFFGVPKETVLTLLPTAIIPFNAIKT
                     VANSILTFLLYKRVEKLLKKEIIKSPVKQAGTLPQEVDNSSTGDSSVNH"
     gene            1384..1782
                     /locus_tag="PLR57_05120"
     CDS             1384..1782
                     /locus_tag="PLR57_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQQ40871.1"
                     /translation="MRYSHQRERIYRAVVESHEHPTANMVYEQLKAEMPKLSLGTVYR
                     NLNQLADAGRLKKIPLADGSCRFDKTTDAHSHIVCEQCGCVADVELPSFEAFEKAISE
                     ETGYTLRSYDVVLRGLCKTCEQAVAGKTLT"
     gene            1905..>2096
                     /locus_tag="PLR57_05125"
     CDS             1905..>2096
                     /locus_tag="PLR57_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQQ40872.1"
                     /translation="MELKGSKTERNLMEAFSGESQARNKYTYFSSVAKKAGFEQISAI
                     FAETAENEKEHAKLWFKALH"
BASE COUNT          525 a          514 c          573 g          484 t
ORIGIN      
        1 gacgaacgcg ttcttctgca gggcgtgatg gacgcctgct ttgtggaaaa cggcgcctgg
       61 acgattctcg actacaagac agaccgcgtc cgtcccggcg agagcgcgga gcaggcggcg
      121 caaaggcacg cgcttcagct ttctctctat gcgctcgcgc tggaagcgct gaccaagctg
      181 ccagtcaaag agaaactcgt cgtaatgctt tcttatcggg cggtaattgc cgtataaagg
      241 cacatttttt ccataatcgc tcttgcaaag gacaatcatt cgtgctacga taggcgcgag
      301 ttgaggggaa acctcccctg acaaacaatt gcatatagct tcgggcgggg tgcaattccc
      361 caccggcggt gaagcggcag ttctgccgac aagcccgcga gtcttatatt aagaccgaga
      421 cggttcgaat ccgtcgccga cggtaagcca gagttttctg acgagtccgg atgaaagaag
      481 cgaaacgcca tgattttcat tggcatccgt tcaccccgcg ttgactagac gcggggtttt
      541 tcgtttcgaa cagtactgct atggagggtt attttatgaa acaaaaatcg ctggccgacc
      601 gattggccaa actcggcatg ctcaccgcgt tgtcggttct gcttgtgtac gcaattcact
      661 ttccaatctt tcccgccgcg tcgtttctgg aatacgacat ggcggatgtg ccgattctga
      721 tcgggacgtt cctttacggc ccgtggtggg ggttggcgct gacggcggta gtgagcctgc
      781 tgcagtggct gctcgtttcg ccgcagagca tgtgggtcgg cgcggtcatg catttctgcg
      841 cgacggggtc atacgtgatt gcggcggggc tcatctatgg aaaaatgcgt acgctcaagg
      901 gcgcgctcat cggcatggcg gtcggttcgg tactgcagac ggctatgatg attccgatga
      961 acctgatctt tacggtgcac ttcttcggtg tgccgaagga aacggtgctc acgctgttgc
     1021 ctacggcgat catcccgttc aacgcaatta agacggttgc gaactcaatt ttgacgttcc
     1081 ttctgtataa gcgcgtggaa aaactgctca agaaggaaat catcaagtcc ccagtaaaac
     1141 aggccggaac gctgcctcaa gaagttgata attcttctac aggggattcc tccgtcaacc
     1201 attgatctca tataaacgat gcgcgccaaa attcaggaac ggcgcgcatt tttcgtattt
     1261 ttgcaggaaa agaacaaaaa ggtattgaca tccaattgga aacgatttag tataataaca
     1321 gtaatcgtta ttgtataaaa aactttgtgt aacgcaacaa taaaacagat ttggagatgc
     1381 tttatgcgct actcccatca gagagaacga atttaccgcg cggtagtgga aagccatgaa
     1441 cacccaaccg ccaatatggt gtatgagcag ctaaaggcgg aaatgccaaa gctcagcctt
     1501 ggcacggttt accgcaatct caaccagctt gcggacgccg gtcggctcaa gaagattccg
     1561 ctggcggacg gcagctgccg gtttgacaaa acgactgacg cgcattcgca tatcgtctgc
     1621 gaacagtgcg gctgcgtggc ggatgtggaa ctgccttcgt ttgaagcgtt tgaaaaggcg
     1681 atttccgagg aaaccggata tacacttcgc tcgtacgacg tggttctccg cggcctatgc
     1741 aaaacttgcg aacaagccgt agccggaaaa actttgacgt aacaaagcct gattttggtg
     1801 aggttacggc ggtaatgccg tttacttcgc tgaaaacggg cgctttcacg cgaacacaag
     1861 aattcaattt tgcaataaaa tcaataatag gaggatgaac tagcatggaa ctcaaaggca
     1921 gcaagacgga acggaatctg atggaagcgt tttcgggcga atcccaggcg cgcaacaagt
     1981 atacgtattt ttcgtcggta gcgaagaaag cgggcttcga gcagatttcc gccatttttg
     2041 cggagacggc tgagaacgaa aaagaacacg ccaagctctg gttcaaagcg ctgcac
//
LOCUS       DAOMGY010000288         2094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_60097_length_2094_cov_3.229524, whole genome shotgun sequence.
ACCESSION   DAOMGY010000288 DAOMGY010000000
VERSION     DAOMGY010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60097_length_2094_cov_3.229524"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLR57_05130"
     CDS             complement(<1..231)
                     /locus_tag="PLR57_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQQ40873.1"
                     /translation="MLYTVFHLNYNAAWVLISVLISFFITLAAIQKGLSRLPKDQGRA
                     YAVNGELSKGKPRGAGLIFSICSTAVFLLFIPF"
     gene            493..963
                     /locus_tag="PLR57_05135"
     CDS             493..963
                     /locus_tag="PLR57_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HQQ40874.1"
                     /translation="MKLKKTAWDWITETLSLALVLGTILYLILMWKAIPDTIPAHYNA
                     AGEVNRWGGKSELLMLPIIGGILYFFITLIQQYPQAWNTGVTVTEENRERVYRILRNL
                     IGTTKLMMLLVFSSLTVLSSLGLALPIWYLGVFLVLLFGSITFFIIQLSKERQK"
     gene            complement(1049..>2094)
                     /locus_tag="PLR57_05140"
     CDS             complement(1049..>2094)
                     /locus_tag="PLR57_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HQQ40875.1"
                     /translation="VSALFVLTYTFLGGFLAESTSDFMQAVVMILALVLVVALGVAAA
                     GGISAVIANAEQIPGFLSLLSSATPTGEAANGYALFGAAKQYSGLAIASAMAWGLGYF
                     GMPQVLLRFMGIRSEHELTRSRRIATIWVVISLATAVLIGLIGRVLFSDAYMVYGSGN
                     TENIFILMSTKMLPPLLAGLVCAGILAAAISSSDSYLLISASAVSKNVWQGLIRKNAS
                     EKETMWVSRIVLVLITVFAIFVATGKNKVIFSIVDFAWAGFGAAFGPLMLFSLFWKRT
                     TYAGAIAGMISGGGMVFLWNFVISKLGGVWGIYELLPAFLFSCLTIVVVSLLTKKPNE
                     AIQKEFDSVNVKA"
BASE COUNT          511 a          557 c          625 g          401 t
ORIGIN      
        1 aaacggaata aagagaagaa acaccgccgt cgaacagatg ctgaagatca gccccgcgcc
       61 gcgcggcttg cccttgctga gttcgccgtt gaccgcgtac gcgcgcccct gatccttggg
      121 gagccgggaa aggccctttt gaatggcggc cagggttatg aaaaaggaga tcaatacgct
      181 gatcaaaacc caggcggcgt tatagttcaa gtgaaaaact gtatatagca tgcatgcctc
      241 gccggtaaaa aatcaaaaca agtatacagc ccaaacggga tatccgcaac ccttttccgc
      301 cgttttgcgg cttgcgcgtt cccatatggc ggggaagaag ggcgcgtttg ccgttcattt
      361 ggcagtggtt ctgttggaac ggggagccgg atacggggct tatgttcttc ccgattcgaa
      421 gaaaacatga tagcatgagg cgaagattcg ggaaagcaag caagacgttt cccgatacga
      481 ctggagaacc gtatgaagct gaaaaagacc gcgtgggact ggattacgga gacgctgagc
      541 cttgctctgg tgcttggaac gatcctgtat ctcatcttga tgtggaaggc gattccggat
      601 accattccgg cgcattacaa cgccgcgggg gaggtgaacc gctggggcgg caaaagcgaa
      661 ctgctgatgc tgccgattat aggcgggatt ctttactttt tcatcacgct gatacagcag
      721 tatccgcagg cttggaacac cggggtaacg gtcacggagg aaaaccgcga gcgcgtttac
      781 cggattttgc ggaatctgat cggcacgaca aagctgatga tgcttctggt gttttccagc
      841 ctgacggtgc tttcgtctct gggtctggcg ctgcccatct ggtatttagg tgtgtttctt
      901 gttttgctgt ttggctctat tacgttcttt atcatacagc tttccaaaga gagacagaag
      961 taagcgccac tattcagatt tatacgatct gcaaagaaaa accagcccgg gcaaagacaa
     1021 ccgggctggt tttgcgttta cgcaatgctt aagccttgac gttgacggag tcgaactcct
     1081 tctgaattgc ttcgttcggc ttcttggtca gcagcgagac gacgacgatc gtcaggcagg
     1141 agaacaagaa cgcgggcagc agctcgtaga tgccccagac gccgccgagt ttgctgatga
     1201 cgaagttcca gaggaacacc atgccgccgc cggagatcat gcccgcgatg gcgccggcgt
     1261 aggtcgtccg cttccagaac agcgaaaaca gcatcagcgg gccaaacgcc gcgccaaagc
     1321 ccgcccaggc aaagtccacg atggaaaaga tgaccttgtt cttgccggtg gcaacgaaga
     1381 tggcgaacac cgtgatgagc acgagcacga tgcgggaaac ccacatggtc tccttttcgg
     1441 aagcgttctt gcgaatcagc ccctgccaga cgttcttgga gacggcggag gcggagatga
     1501 gcaggtagga atcggaagag ctgatcgccg cggcaagaat acccgcgcag acgagacccg
     1561 ccaggagcgg cggcagcatc ttggtggaca tcaggataaa gatgttctcc gtgttgccgg
     1621 agccgtatac catgtacgcg tccgaaaaca gcacgcgccc gatcaagccg atgagcacgg
     1681 cggtggccag cgaaatgacg acccaaatcg tcgcaatccg gcgcgagcgg gtcagttcat
     1741 gctcggagcg gatgcccata aagcgcagca gtacctgcgg catgccgaag tagccgagcc
     1801 cccaggccat ggcggaagcg atggcgaggc cggaatactg ctttgccgcg ccaaagagcg
     1861 catatccgtt tgccgcttca ccagtcggcg ttgcggagga aagcaggctc aaaaagccgg
     1921 gaatctgctc ggcgttggca atgaccgcgc tgatgccgcc agcggcggca acgcccaaag
     1981 cgaccaccag taccagcgcc agaatcatga ctacggcctg cataaagtcc gacgtgctct
     2041 cggcgaggaa cccgccgagg aacgtatagg taagcacaaa cagagcggat acga
//
LOCUS       DAOMGY010000289         2086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_60440_length_2086_cov_2.525849, whole genome shotgun sequence.
ACCESSION   DAOMGY010000289 DAOMGY010000000
VERSION     DAOMGY010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60440_length_2086_cov_2.525849"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLR57_05145"
     CDS             <1..477
                     /locus_tag="PLR57_05145"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQQ40876.1"
                     /translation="KYYKREGVLTGVAGRKGFTVISVYKDNMHNEVGFGYRLLRVLDR
                     YNISFEHIPSGIDTMSLVIQETKLKEHLEQVLREIQRDCEPQSIEVHSNMALVATVGK
                     GMIRSLGVAARLFGALYEAGVNVRMIDQGSSEMNIIVGIENDDLPTAVRAIYRAFA"
     gene            600..845
                     /locus_tag="PLR57_05150"
     CDS             600..845
                     /locus_tag="PLR57_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40877.1"
                     /translation="MEVKKRVLHHGTHYNAPIDWNDPEQAAAQVKIDLLRNMLACNPM
                     SYSEKEELIRKQLAEEAHREEAEQAARSNPSGKKWDI"
     gene            1059..1718
                     /locus_tag="PLR57_05155"
     CDS             1059..1718
                     /locus_tag="PLR57_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40878.1"
                     /translation="MKKRFLCAALAAMLISFSLSGCAAINAAAAPGEVEEFQIGTIEG
                     NTYSNEFFGIGCQLDDTWKVLNEEEIAERSGLVVDTMEEGGLDDSMRTAMENGSVVFD
                     FFAQKKDNTAAINITVGNIGILYGTAMTEEGYIDATTEQMTESIEAIGYENVTTEKTT
                     ITLAGTEHAALKLSGEFSGISIYQTMVVLRKGIYVGCVAFSCGGEDFADDLITLFYAL
                     D"
     gene            complement(1846..>2086)
                     /locus_tag="PLR57_05160"
     CDS             complement(1846..>2086)
                     /locus_tag="PLR57_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQQ40879.1"
                     /translation="LFIVEAAQRAMESVGVKPHLAPIRGGTDGSRLSYVGLPCPNLCT
                     GGYNFHGRYEFISVEAMEKVVDILQALVTSFVPAK"
BASE COUNT          523 a          530 c          601 g          432 t
ORIGIN      
        1 aagtattaca agcgcgaagg tgtgctcacc ggcgtagcgg gccgcaaggg ctttacggtt
       61 atcagcgtgt acaaggacaa catgcacaac gaggtcggct ttggctatcg tctgttgcgc
      121 gtgctggatc gctacaatat ctcgtttgag catatcccca gcggcatcga caccatgagc
      181 ctcgtgattc aggaaaccaa gctcaaggaa catctggagc aggtgctgcg cgagattcaa
      241 cgtgactgcg agccgcagag catcgaggtg cattccaaca tggcgctggt cgccacggtc
      301 ggcaagggca tgatccgctc gctgggcgtt gcggcaaggc tgtttggcgc gctgtacgaa
      361 gcgggcgtga acgtccgcat gatcgaccag ggctcctcgg agatgaacat catcgtcggc
      421 attgaaaacg acgacctgcc gactgccgtg cgcgccatct accgcgcgtt tgcgtaaggc
      481 aaaacggctt tttcagcgta caggctcctc cgcaaggggg agcttttttg cgttgtccgg
      541 cgcgcattga caatccatcc cggcaggaat agaatgaggg caatccaaac agaattgaaa
      601 tggaagtgaa gaaacgcgtg ctccatcatg gtacgcatta caacgcgcca atcgattgga
      661 acgatccgga acaggcggca gcacaggtta aaatcgacct gctgcgtaat atgcttgcgt
      721 gtaatccaat gagctattcc gaaaaggaag agctgattcg aaaacagctt gccgaagaag
      781 cgcaccgcga ggaagcggag caggcggcaa ggtcgaatcc ttcgggtaaa aaatgggata
      841 tctagccaac ggcttctcag aagaatgata gcagtttatc aaccaaactc tcccgccggg
      901 gagagttttt tactacataa aacagccaaa aacaaggcgt acggcacaca aatgcgcgac
      961 taatgtacga acgtacagtg cggtttatcc aaaacgtaca gttgtttccc aaaacacctt
     1021 tatggtaaac tgaataccat caatactcgg ggggatttat gaagaaacgt ttcctttgcg
     1081 cggctctggc cgcaatgctc atcagttttt cgctttccgg ctgcgccgca atcaacgcgg
     1141 cggcggcgcc cggcgaggtg gaagaattcc agatcgggac aatcgaaggc aatacctaca
     1201 gcaacgagtt tttcggcatc ggctgccagc tggacgacac ttggaaggtt ctgaacgagg
     1261 aggagattgc cgagcgcagc ggacttgtgg ttgacaccat ggaagaaggc ggcttggacg
     1321 acagtatgcg cactgcaatg gagaacggca gcgtcgtttt cgacttcttt gcgcaaaaga
     1381 aggacaacac cgcggccatc aacatcaccg tgggcaacat cggcatcctc tacggtacgg
     1441 ctatgaccga ggaaggctat atcgacgcga caacggagca gatgacggag tccatcgaag
     1501 cgatcgggta tgaaaacgtc accacggaaa agacaacgat cacgctggcg ggaaccgagc
     1561 atgccgcgct gaaactttcg ggtgaattct cgggcatatc gatttaccag accatggtcg
     1621 tgctccgcaa gggcatctat gtcggctgcg tagcgttctc ctgcggcggc gaggactttg
     1681 cagacgatct gataacgctg ttctacgcgc tggactagga acgaatcgaa aaaaggagct
     1741 ttccaattgt ggaaagctcc tttttttaat gtttgacttc gaccagatca aatcgttttt
     1801 tggagagctc cttttttagt gcttgaattc gattagatca aaccgttatt ttgcagggac
     1861 gaacgaggta acaagcgcct gcaggatgtc cacaaccttc tccatggcct ctacgctgat
     1921 gaactcgtat ctcccgtgga agttgtatcc gcccgtgcag aggttggggc acggcaggcc
     1981 tacgtaggag aggcgggagc cgtccgtgcc gccgcggatg ggtgcgaggt ggggcttgac
     2041 tccaacgctc tccatggcgc gctgagcggc ctctacgatg aagaga
//
LOCUS       DAOMGY010000290         2084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_60502_length_2084_cov_3.912272, whole genome shotgun sequence.
ACCESSION   DAOMGY010000290 DAOMGY010000000
VERSION     DAOMGY010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60502_length_2084_cov_3.912272"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLR57_05165"
     CDS             <1..368
                     /locus_tag="PLR57_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQQ40880.1"
                     /translation="TVAPVICGASLKVLDLLAGSPALRDKLHENKRYFRENLGKLGFD
                     VLPGEHPIVPVMLYDAKTAQEFAARMLEKGVYVVGFFYPVVPMGKARIRTQVSAAHSK
                     ADLDFAVSCFAEVKREMGL"
     gene            complement(454..1869)
                     /locus_tag="PLR57_05170"
     CDS             complement(454..1869)
                     /locus_tag="PLR57_05170"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQQ40881.1"
                     /translation="MKQSNSTPEYNREGLLTEKTNLNFRSWQLPAVIAFICCIFISVY
                     QHYAPKYADLFLSSVLFPASVGIFALVLYTRPFLKYAEVKVMGVLFIWMILGLLFNDW
                     RLEKAFTSYWLYCACITTFLCFSLPYAFDKAMLKKALSILAAAMLVSATLLSAAALAI
                     VFGGVQAPAALTKIGYVGIHTDGRLWMVLHPNSIGPICGAAIILTGYLLTRTKKWLVR
                     VLLILCAVACYSTMALTDSRTAFISTAIAVALEFFLLFNLSFSKAKRKVGRILLSLAV
                     TLLAVGIFYFGTTVVRSVYNNTVVACAATESTASSTVTAQTDTVVDRGLDKVDSFNGR
                     TDIWKGTLQGLKENPDILLTGTTPIISGKVLASYFPEGSPIGNFHNSFVASLVSFGIP
                     GFIVILAFFVMLAISSARLSFTKLLDKDSLGARLIPAILLFAVSESMMEVFLFVEFSL
                     NVVWIWFMFAAGFVFRMQKSE"
BASE COUNT          542 a          541 c          565 g          436 t
ORIGIN      
        1 ataccgtcgc tcccgtcatc tgcggggcaa gcctgaaagt gctcgattta ctggcaggat
       61 cccctgcgct gcgcgacaag ctgcacgaaa acaagcggta tttccgcgaa aacctcggca
      121 agctcggctt cgacgtgctg cccggcgaac acccgatcgt acccgtaatg ctctacgacg
      181 cgaaaaccgc acaggaattc gccgcgcgga tgcttgaaaa gggcgtttac gtcgtgggct
      241 tcttctatcc ggttgtgccg atgggcaaag cgcgcatccg cacgcaggtt tccgccgcgc
      301 acagtaaggc cgacctcgac tttgccgttt cgtgtttcgc ggaagttaag cgcgagatgg
      361 ggctgtaact gcaaagaaat tcctgcatat taagaatagt aataaaaggg ctgccaaact
      421 tggcagccct tgctgtttct gcatttaaat gtgttactcg ctcttctgca tgcgaaagac
      481 gaagcccgcg gcaaacataa accagatcca gacaacgttc agcgagaatt cgacaaacag
      541 gaagacctcc atcatcgatt ccgaaaccgc aaacagcaga atcgccggaa tcagacgcgc
      601 gccaagggaa tccttatcca gaagctttgt aaaggacagc cgcgcggaag agatcgccag
      661 cattacaaag aatgccagaa tgacgatgaa ccccggaatg ccgaacgaaa cgagtgaagc
      721 cacaaacgag ttgtggaagt taccgatggg cgaaccttcc gggaaatacg acgccagcac
      781 cttgccggag ataatggggg ttgtgccggt caggaggata tcgggatttt cctttaatcc
      841 ctgcaacgtc cccttccaaa tatccgttcg tccgttaaac gaatcaactt tatccaaacc
      901 cctgtccaca accgtatcgg tctgcgcggt tacggtactg ctggcagtac tctctgttgc
      961 agcgcaagcg acgaccgtat tgttgtacac ggagcgcacg acggtcgttc cgaaatagaa
     1021 gatgcctacg gccagcagcg tgacggccag gctcagcagg atgcggccaa ccttccgctt
     1081 cgccttggaa aaggacaggt tgaacagcag aaagaactcc agcgcaacgg cgatggcggt
     1141 gcttataaag gcggtgcggc tgtccgtcag cgccatggtg gagtagcagg cgacggcaca
     1201 gagtatcaat aaaacgcgta caagccattt cttcgttcgg gtgagcagat atcctgttag
     1261 gataattgca gcgccgcaga ttggcccgat gctgtttgga tggagaacca tccaaagcct
     1321 gccgtcggtg tgaatgccga cataaccgat tttcgttaga gcagccggcg cttgtacgcc
     1381 gccgaacaca atagccagtg cagctgcact taacaatgtt gcggagacga gcatagccgc
     1441 ggcgagaatg gagagcgcct tttttagcat tgctttgtcg aacgcatagg gaagcgaaaa
     1501 gcagagaaac gtcgtaatgc aggcgcagta cagccagtag cttgtaaacg ccttttccag
     1561 ccgccagtcg ttgaacagca gcccgagaat catccagata aacaggacgc ccatgacctt
     1621 tacttccgca tatttcaaaa acgggcgcgt atagagcacc agcgcaaaga tgcccaccga
     1681 agcgggaaag agaacgctgg aaaggaataa atcggcgtac ttcggcgcat agtgctggta
     1741 tacgctgatg aaaatgcagc aaataaacgc aatgacagcg ggaagctgcc aggagcggaa
     1801 attcaggttc gttttttcgg tgagtagtcc ttcgcggttg tattccggcg tggaattcga
     1861 ctgtttcatg tttcttcctc tgcggttttc gtttacaatt tgctggttct gccaggctgc
     1921 ctattttacc ggccgggtat gaggcgggtg tgcgtttgaa gcacgtatag acaatcctgt
     1981 gaacagcacg cctaatggta caccgccgga tgcagagcgt caatacaaaa accagcaaga
     2041 atagttagaa ttcactaaag tttttcatgt cgttaaccgg tgca
//
LOCUS       DAOMGY010000291         2084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_60510_length_2084_cov_3.603746, whole genome shotgun sequence.
ACCESSION   DAOMGY010000291 DAOMGY010000000
VERSION     DAOMGY010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60510_length_2084_cov_3.603746"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLR57_05175"
     CDS             complement(<1..407)
                     /locus_tag="PLR57_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40882.1"
                     /translation="MRFLRKWLIPMIAGVLLLCLIAAFIPLTTREETRAKDDLSQLMD
                     TAFTRIAASQSAADPVVAAEQASLLSKARAVTRFLEHDDTLLATDALAALCSQLSIGQ
                     IDVANAEGTLIASSNASSIGTAPGTQEGFEWVL"
     gene            complement(545..1252)
                     /locus_tag="PLR57_05180"
     CDS             complement(545..1252)
                     /locus_tag="PLR57_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40883.1"
                     /translation="MNFIDIAILLVLGITILGGLYRGFVSTVYNVGATIVSLIVGRIC
                     IPLVSGLVKGSADLFNMMLYYTEGSEYVAITDVELTRTSIASISSDQLHTVLQNADMP
                     IPMDRCVIKNIAQEAFRDSGVTTLGDYFNQTIVCVVINILAMVLIFAVVRLLLGFVIR
                     GIEYGRGGFPVLTRGDGIIGAGLGLIHGVLLLFAIFMLVPVMLTVLPKLYEFLSESFF
                     GEFFYQANFLLSLIPAT"
     gene            complement(1280..>2084)
                     /locus_tag="PLR57_05185"
     CDS             complement(1280..>2084)
                     /locus_tag="PLR57_05185"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HQQ40884.1"
                     /translation="MTVVFNEAQLQVVGEKTPLTFTGFIREVECGVKHLAVLRQAEDG
                     SESVLIITKDGEQIKDLTFEDQYIVDFGFYTAKNEMMWIETLNINTGTPSTTISTYDF
                     VQQEITGLIHVQNQLVDQIYITPDSMFVVATNQIIRYTHEGNKEIYRMTIYGYKAVDF
                     SNASGTPTFLLTTRGGDFHTVKIMTLEEGTEASPVETTLQLPTEGVAAFIMGSRLEVV
                     SRISLYTYTLKGKLSATATFQQPVDEAVKLTDTKLLLSSNGMYYIGNVG"
BASE COUNT          461 a          597 c          562 g          464 t
ORIGIN      
        1 tccagcaccc attcaaaccc ttcctgcgtc cccggcgcgg tgccgatgct tgatgcgttg
       61 gaggatgcaa tcagcgttcc ttccgcgttg gcaacgtcga tttggccgat gctgagctgc
      121 gaacagagcg ccgcgagcgc gtcagttgcc agcagggtat cgtcgtgttc cagaaaacgc
      181 gtcactgcgc gtgctttgct caaaagagaa gcctgttcag cggcgacgac cggatcggcg
      241 gcggattgag aagccgctat ccgcgtaaac gccgtgtcca tcagttgcga cagatcgtcc
      301 ttagcacgcg tttcttcccg cgtggtgagc ggtataaaag ccgctatcag acacaggagc
      361 aatacccccg caatcatggg tatcagccac ttccgtaaaa aacgcacctt gtttcgaccg
      421 cctttcccgg gttccgtttt cagccgccgc gcggtgtttt caccaaacga acagcatttt
      481 caatctaatc tcatcagcac ggcgttgtaa tgccttcagg ccgtttgctc cttcgccgca
      541 tacgttacgt ggcgggtatg agcgaaagca ggaaattggc ctgataaaag aactcgccaa
      601 agaacgattc gcttaaaaac tcgtagagtt tcggcagaac cgtcagcatg accggcacaa
      661 gcatgaaaat agcgaacaac agcagcacgc cgtggatcag cccgagcccc gcgccgatga
      721 ttccgtctcc gcgcgtgagc accggaaaac cgccgcgccc gtactcgatt ccgcgaatca
      781 caaagccgag cagcaggcgc acaacggcaa atatcagcac cattgcgaga atattgatga
      841 ccacgcagac gatcgtctgg ttaaagtaat cgccgagcgt cgttaccccg ctgtcgcgga
      901 acgcttcttg agcgatgttt ttaatcacgc accggtccat cggaatcggc atatcggcgt
      961 tttgcagcac ggtatgaagc tggtcggaag agatgctggc aatggacgtg cgcgtcagct
     1021 ccacgtccgt aattgcaaca tattccgaac cctccgtgta atagagcatc atgttgaata
     1081 agtccgcgct gcccttgacc aggccggata ccagcggaat gcagatgcgt cccacgatga
     1141 gggaaacaat cgtagctcca acattgtaaa cggtactgac gaacccgcgg tacagcccgc
     1201 ccaggatcgt aattcccagc accagtaaaa ttgcgatgtc gataaagttc attgtctcgc
     1261 cttttcagcc cgcaaggaat cagccaacgt tgccaatgta gtacatgccg ttgctggaga
     1321 gcagcagctt ggtgtccgtg agtttgaccg cttcgtcaac cggctgctgg aacgtcgcgg
     1381 ttgcggagag tttgcctttg agcgtgtagg tatagaggct tatgcgggag acaacctcca
     1441 gcctgctgcc catgatgaat gccgcaacgc cttccgtcgg cagctgcagc gtcgtttcca
     1501 cggggctcgc ctcggtgcct tcctccagcg tcatgatctt aaccgtgtgg aagtcgccgc
     1561 cgcgcgtcgt gagcagaaac gtcggcgtgc cggaagcatt ggagaagtca accgccttgt
     1621 aaccgtaaat cgtcatgcgg tagatttcct tgttgccctc gtgcgtgtag cggataatct
     1681 ggtttgtcgc aacaacgaac atgctgtccg gcgtaatgta aatctgatcg accagctggt
     1741 tctgcacgtg gatgaggccg gtaatctcct gctgcacgaa gtcgtaggtc gaaatcgtgg
     1801 tcgagggcgt tccggtgttg atgttgagtg tttcgatcca catcatctcg tttttcgccg
     1861 tgtagaagcc gaagtccacg atatactgat cctcgaatgt gaggtccttg atctgctcgc
     1921 catcttttgt gatgatgagc acgctctccg aaccgtcctc ggcctgccgc aaaacggcaa
     1981 ggtgctttac gccgcattct acctcacgga tgaaccccgt aaacgtcaac ggcgtttttt
     2041 cgccgaccac ctgcagctgc gcttcgttga acacaaccgt catg
//
LOCUS       DAOMGY010000292         2084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_60524_length_2084_cov_2.694924, whole genome shotgun sequence.
ACCESSION   DAOMGY010000292 DAOMGY010000000
VERSION     DAOMGY010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60524_length_2084_cov_2.694924"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLR57_05190"
     CDS             <1..796
                     /locus_tag="PLR57_05190"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HQQ40885.1"
                     /translation="VNGITSRGGTILYTARSEEFKTEAGMQAAAANCKYLGIDGIIAI
                     GGDGTFRGARDLARLGVNTVGIPGTIDNDIACSHYSIGFDTAANTAIDAIDKLADTMQ
                     SHQRTSVVEIMGRNAGHLAIYVGISVGATSILIPEHPFDFEKDVVEHIREGQYRGKHH
                     HLIIVAEGVGHTNEIAERIRTELGLDTRVTIIGHVQRGGSPSARDRVMATRMGHRAVE
                     ELIAGGSNLIVCYRNSQITTVPIDEALEMTKGLDDYMYRVSQEITI"
     gene            990..1727
                     /locus_tag="PLR57_05195"
     CDS             990..1727
                     /locus_tag="PLR57_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ40886.1"
                     /translation="MYFDHYGKEGSPVLVLLHGAAALDTFAGHYAALSEHFHVLVPHL
                     PGAGQAAEEAYDPQAVCDALAEWIGSLSAGKVALMGHSVGAELAFKIVCEHESLFSRA
                     VFLSPWLMAGSASTKLYVSMARMTYKSIKNEKLIRMQAKYWKLSPKQADAMVAYSQKI
                     PLDTYVSFFEERIHFSDEPGYESVSIPMLAMCAKGDSGETKASVYALGENKNCLTVIF
                     PLGSHDFVLRQAEKLNPMLLDFLLAPA"
     gene            complement(1724..>2084)
                     /locus_tag="PLR57_05200"
     CDS             complement(1724..>2084)
                     /locus_tag="PLR57_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40887.1"
                     /translation="LIRWTILRGLAGTSLFRLKRMLKRHGYPTALKIVGKKNVALPEG
                     TEAGVIYYVHKDGPHFVTFYCDETVLQQENEKPRYRFLNAIPGRGNHFDTMQGFLTKH
                     NVLPIAGILVYPRKASS"
BASE COUNT          431 a          657 c          577 g          419 t
ORIGIN      
        1 cgtcaacggc atcacctcgc gcggcggcac gatcctctac accgcccgca gcgaggagtt
       61 caagaccgaa gcgggcatgc aggcggccgc cgcaaactgc aagtaccttg gcatcgacgg
      121 catcatcgcc atcggcgggg acggcacctt ccgcggcgcg cgggatcttg caaggctcgg
      181 cgtcaacacc gtcggcatcc ccggcacgat cgataacgac atcgcctgct cgcactattc
      241 catcggcttc gacaccgcgg ccaataccgc catcgacgcc atcgataagc tagcggacac
      301 catgcagtcc caccagcgca cctccgtggt cgaaatcatg gggcgcaacg ccgggcatct
      361 tgccatctat gtcggcatct ccgtcggggc tacctccatt ttgattcccg agcatccgtt
      421 cgattttgaa aaggacgtcg tggagcacat ccgcgaaggc cagtaccgcg gcaaacacca
      481 tcacctgatc atcgttgccg agggcgttgg ccataccaac gaaatcgccg agcgcatccg
      541 caccgaactg ggtctggata cgcgcgtcac catcattggc cacgtacagc gcggcggcag
      601 ccccagcgcg cgcgaccgcg tgatggcgac ccgcatgggt caccgcgccg tggaagagct
      661 gattgccggc gggtcgaacc tgatcgtctg ctaccgcaat tcgcagatca caaccgtgcc
      721 catcgacgaa gcgcttgaaa tgaccaaggg gcttgacgac tacatgtacc gcgtctcgca
      781 ggagattacg atataagggg gtcttcttgg ggatttcatc cccaaacctg aaaaagacgg
      841 gcagtggctc cgcccccgca cccccagacc aaagggcagt cgccctttgg aaacccctaa
      901 gacttataaa agctcggacg aaagttcggg ctttttgatt ctgacgtggt atactgttgt
      961 cagcaaaatc gtgttttacg gaggccatta tgtacttcga tcattacggc aaagaaggca
     1021 gtcccgtgct cgtgcttctg cacggagccg ccgcgctgga tacgttcgcg gggcattacg
     1081 cggcgctttc ggagcatttc cacgtcctcg ttccgcatct gcccggcgcg gggcaggccg
     1141 cggaggaagc ttacgacccg caggcggtct gcgacgcgct tgccgagtgg atcggctcgc
     1201 tcagcgcggg caaggtcgcg ctcatgggtc actccgttgg cgcggaactc gcgttcaaaa
     1261 tcgtctgcga gcatgaatcg ctcttttccc gcgcggtgtt cctcagcccc tggctcatgg
     1321 cgggttccgc cagcacaaag ctctatgtct ccatggcgcg gatgacctac aagtccatca
     1381 aaaacgaaaa actgatccgc atgcaggcca agtactggaa gctatccccg aagcaggcgg
     1441 acgcgatggt tgcctacagc cagaagatcc cgctggacac ctacgtttcg ttcttcgaag
     1501 agcggattca tttttccgac gaaccgggat acgagtccgt ctccatcccc atgctcgcca
     1561 tgtgcgccaa aggcgactcc ggcgaaacga aagcttccgt ttatgctttg ggcgaaaaca
     1621 aaaactgcct cacggtcatc tttccgctcg gcagtcatga ttttgtgctt cgtcaggctg
     1681 aaaagctcaa cccgatgctg ctggatttcc tgctcgcccc cgcttaagac gaggcttttc
     1741 gagggtatac caaaatcccc gcgatgggca gaacgttgtg ctttgtcaaa aacccctgca
     1801 tcgtgtcaaa atgattcccg cgccccggta tcgcgttcaa aaagcggtag cggggttttt
     1861 cgttttcctg ctgcagaact gtttcgtcgc agtaaaacgt aacgaagtgc gggccgtcct
     1921 tatggacata gtaaatcacg cccgcttccg ttccttccgg cagggcgacg ttctttttgc
     1981 ccacgatctt cagcgcggtc gggtacccgt gccgcttgag catgcgctta agccggaaga
     2041 ggctcgtgcc cgccagcccg cgcagaatcg tccatcgaat cagt
//
LOCUS       DAOMGY010000293         2080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_60701_length_2080_cov_2.999012, whole genome shotgun sequence.
ACCESSION   DAOMGY010000293 DAOMGY010000000
VERSION     DAOMGY010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60701_length_2080_cov_2.999012"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1235)
                     /gene="aroB"
                     /locus_tag="PLR57_05205"
     CDS             complement(165..1235)
                     /gene="aroB"
                     /locus_tag="PLR57_05205"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HQQ40888.1"
                     /translation="MDMRRIRVQVQNGYDVCIGRGLTAGFGGLLRSAVGSCRVAVMTD
                     SNVAPLYLDALTQSLEQAGYDVCAFVFPAGESSKRMDTLAEILEFLVENRFTREDCVI
                     ALGGGVTGDLTGFAAGCYLRGIRYVQAPTSLLAAVDSSVGGKAAVDLSAGKNLAGLFY
                     QPSLVVCDTNCFATLPKSEIANGSAEAIKTGVLGGETLFGLFESGAAREQMEQVVAQC
                     VAYKARIVEADERDSELRNILNLGHMAGHAIEVCSEYAIPHGQAVAIGLGIIARASER
                     LGEAQEPIYERIRQTLVKNGLPVVSPYSPEELAAAALSDKKRAGDSIRIVLPRAIGNC
                     VLKNVPVSELISIFRAGMEDDA"
     gene            complement(1240..>2080)
                     /locus_tag="PLR57_05210"
     CDS             complement(1240..>2080)
                     /locus_tag="PLR57_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HQQ40889.1"
                     /translation="KMNVGIVGLGLIGGSMAKSIKLRTAHTVWGVDLDAETMTLARLS
                     GAIDAPLDGGNLGLCNLILIAIRPNAALEWVRSHADEIGKGAIMVDLCGVKRTVCAEL
                     APIAREKGFSYIGGHPMAGRERSGFVSASEELFTNASMILTPDERTDIIMLEKLKSFF
                     TDIGFAGLTFSTPEEHDRIIAYTSQLAHVVSSAYIKSPEAQQRRGFSAGSYRDMTRVA
                     HLDEAMWTELFLDNADYLTEQLDILIQHLQEYQSALCAQDAERLCALLKDGREKKASA
                     GGN"
BASE COUNT          401 a          611 c          649 g          419 t
ORIGIN      
        1 tatgaggcag tcgatagtgg tttggatgtc gtccgaatgg cggttgatct ccacccgcgt
       61 cgtacctttg gaaagtgcgg cgagaatcag aacccggtgc gcgtcggatt tggaaggaat
      121 ggccgcgagt tcgccgccga gaggagaagg ggagatgcaa aggttcatgc gtcatcctcc
      181 atgcccgcgc ggaaaatgga gatgagttcg ctcacaggaa cgttcttgag cacgcagttg
      241 ccgattgcgc gggggagtac gatgcggatg gaatcgcccg cgcgtttttt atcggagagc
      301 gcggcggcgg cgagttcttc cggggaatag ggagatacga ccggaaggcc gtttttcacg
      361 agcgtctgcc ggatgcgctc gtatatcggt tcctgcgctt cgccgaggcg ctcggacgcg
      421 cgcgcgataa tgccaagtcc gatggcgacg gcctgcccgt gcggaatggc gtattcgctg
      481 cagacttcta tggcgtgccc cgccatatgg ccgaggttga ggatgtttct cagctcgctg
      541 tcgcgctcgt ccgcttcgac aatacgagcc ttataggcaa cgcattgagc gacgacctgt
      601 tccatctgct cacgcgcggc tcccgactca aacaggccaa aaagcgtttc gccgccgagc
      661 acgccggtct tgatcgcttc ggcggagccg ttggcgattt cgcttttcgg aagggtggca
      721 aagcaatttg tatcgcatac cacaagcgaa ggctggtaga acagccccgc gaggttcttg
      781 ccagcggaaa ggtccacggc cgctttgccg ccgacggagg agtccaccgc ggctaaaagc
      841 gaagttggcg cctgcacata ccggatgccg cggaggtaac accccgccgc aaagcctgtt
      901 aagtcgcccg tgacaccgcc gccgagggcg atgacgcagt cctcgcgcgt gaaccggttt
      961 tccactaaaa attcaaggat ttccgcgagc gtatccatgc gcttgctgct ttcgcccgcg
     1021 ggaaaaacaa acgcgcagac gtcgtagccc gcttgttcga ggctctgcgt cagcgcgtcc
     1081 agatagagag gggcgacgtt ggaatcggtc atcacggcga cgcggcagga accgactgcc
     1141 gaacggagca gaccgccgaa accggcagtc aaaccgcgcc cgatgcagac gtcgtaaccg
     1201 ttttgtactt gtacgcgaat tctgcgcatg tccatttgtt cagtttcctc ccgccgaggc
     1261 tttcttttcg cgtccgtcct tgagcagggc gcagaggcgc tcggcgtctt gcgcgcagag
     1321 cgcggactgg tattcctgca ggtgctgtat caggatatcg agctgctcgg tcaggtagtc
     1381 cgcgttgtcc aaaaagagct ctgtccacat ggcttcgtcg agatgcgcca cgcgcgtcat
     1441 atccctgtag cttcctgcgg aaaagccgcg ccgctgctgt gcttccgggg atttgatgta
     1501 cgcgctggag accacgtgcg ccagctggga cgtgtaggcg atgatgcggt cgtgctcttc
     1561 cggcgtagaa aacgtcagcc ccgcaaagcc gatatccgta aaaaacgatt taagcttctc
     1621 gagcatgatg atatccgtcc gctcgtcggg agtcaagatc atcgaagcgt tggtgaaaag
     1681 ctcctcgctt gcactcacaa agccactgcg ctcgcgcccc gccatcgggt gcccgccgat
     1741 gtaagaaaac cccttctctc gcgcgatggg cgcaagctct gcgcagaccg ttcgcttgac
     1801 gccgcagaga tcgaccatga ttgcgccctt accgatttca tccgcatggg agcgaaccca
     1861 ctccagcgcg gcgttcgggc ggatggcgat gagaatcagg ttgcacaggc cgaggttgcc
     1921 gccgtcgagc ggcgcgtcga tggcgccgct gagccgggcg agcgtcatgg tttccgcgtc
     1981 cagatcgacg ccccatacgg tatgcgccgt gcggagctta atgctttttg ccatggagcc
     2041 gccgattaaa ccaaggccga cgatgccgac gttcattttg
//
LOCUS       DAOMGY010000294         2077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_60817_length_2077_cov_3.553907, whole genome shotgun sequence.
ACCESSION   DAOMGY010000294 DAOMGY010000000
VERSION     DAOMGY010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60817_length_2077_cov_3.553907"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PLR57_05215"
     CDS             <1..1018
                     /locus_tag="PLR57_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ40890.1"
                     /translation="AYAENAVDNGVRLSLDSAVLGMRVENGEIKSVQTNRGTVYPKVV
                     VNAAGVFSEDVAKMACDRFFSIHPRRGTNTILDSKSAFRLNTIVSSLGTSSTKKKHTK
                     GGGLILTIDGNILVGPDAVETFEKENFATTEKSVTDVFDKQRRTDPALRFGEAITYFT
                     GVRAATYEEDFVIRKGRRTRNIVHAAGIQSPGITAAPAIAVDVAKMAAEVLGHTRPVG
                     KKANFNPIRKGVVRANELAFEERNELIRQNPDYGEIVCRCEQISRGEVLDALHRSVPC
                     DTVDGVKRRVRPGMGRCQGGFCGPLVLRIIAEDKGTSLEEVEKSGIGSELLFGGIKEV
                     TQND"
     gene            1011..>2077
                     /locus_tag="PLR57_05220"
     CDS             1011..>2077
                     /locus_tag="PLR57_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ40891.1"
                     /translation="MTDTASYDVTVIGAGPAGLAAALEADRSGASVLLIEREAQLGGI
                     LKQCIHDGFGLVRFGEKLSGPEYAERFIARLPESGVTVSTLSFVSNIEHVGENFYLTL
                     VTREGMRRVRSRSLVLATGCRERTSRQIGIHGRHCAGVLTAGCAQYYTNILGKLPTKR
                     CVVLGSGDIGLIMARRLTLEGAKVVGVYEAKPTPSGLTRNISQCLTDFEIPLYTSKTV
                     TRVFGNQRLSAVEISDVDEKMRPIAGTEEIIECDALILSVGLIPENELAESLGVPLDG
                     KTKGAVVDEQNQTMLDGVFACGNALHVNDLVDYVSESGETAGRAAAVHAKEPDAKVRD
                     LAPVNCDERLLYVVPQKLDRHA"
BASE COUNT          473 a          601 c          623 g          380 t
ORIGIN      
        1 cgcctacgct gaaaacgcgg tcgataacgg tgttcggctc tcgctcgatt ccgcagttct
       61 cggtatgcgc gtggaaaacg gcgagatcaa gagtgttcag acaaaccgtg gaacggttta
      121 ccccaaggtc gtcgtcaacg cggcaggcgt tttttcggaa gacgttgcca aaatggcctg
      181 cgaccgtttc ttctcgattc atccacgcag aggcacgaat acgatactgg acagcaaatc
      241 cgccttccgg ctcaacacca tcgtctcctc gcttgggact tccagcacga agaaaaagca
      301 caccaaaggc ggcgggctca tcctcacaat cgacggcaat atcctcgtcg gccccgacgc
      361 ggtggagaca ttcgaaaaag agaatttcgc aacgaccgaa aaaagcgtga cggacgtctt
      421 cgacaaacag cgccgcaccg accccgccct acgctttggc gaggcgatta cgtattttac
      481 gggcgttcgc gcggctacct acgaagaaga tttcgtaatc cgcaaaggcc gcagaaccag
      541 aaacatcgtc cacgcggctg ggattcagtc ccccggcatt acggcggctc ccgccattgc
      601 ggttgatgtt gcaaagatgg ccgccgaggt tctcggacat acccgccctg tcggtaaaaa
      661 ggcgaacttc aacccgattc gtaagggagt agtccgcgca aatgaacttg cgtttgaaga
      721 acgcaacgag ttgattcgtc aaaacccgga ctacggcgaa attgtctgcc gctgcgaaca
      781 gatcagccgc ggcgaagtgc tcgacgcgct ccacagaagc gttccctgcg atacggtgga
      841 cggcgtgaag cgccgcgtgc gccccggcat ggggcgctgt cagggcggat tctgcgggcc
      901 gctggtgctg cgtatcatcg ccgaggataa aggtacgtcc ctggaagaag ttgaaaagag
      961 cggcatcggc agcgagcttc tctttggcgg aatcaaggag gtgacgcaga atgactgaca
     1021 cggcatcgta tgacgtaacg gtcatcggcg cgggccccgc aggcctcgcc gccgcgctgg
     1081 aagcggaccg ctccggcgca agcgtccttc tgatcgagcg ggaagcgcag ctcggcggaa
     1141 tcctcaagca gtgcatccac gacggcttcg gtttggttcg tttcggcgaa aaactctccg
     1201 gcccggaata cgccgagcga tttatcgcgc ggctgcccga aagcggagtc accgtgagta
     1261 cattatcgtt tgtaagcaat attgagcacg taggcgaaaa cttctacctg actctggtca
     1321 cgcgggaagg catgcgccgc gtacgaagcc gctcgctcgt gctcgctacc ggctgcaggg
     1381 aacgcacttc ccgccagatc ggtattcacg gccggcattg tgcgggcgtg ctgacagcag
     1441 gctgcgcaca atactacacg aacatcctcg gcaaacttcc gaccaaacgc tgcgtcgttc
     1501 tcggcagcgg tgatatcggg ctgatcatgg cgcggcggct cacgctggaa ggcgcgaagg
     1561 tcgtcggcgt ttacgaagca aaacccacac ccagcggact gacgcgcaac atcagccagt
     1621 gtttgacgga tttcgaaatt ccgctctaca cgagtaaaac cgtgacgcgc gtattcggca
     1681 atcagcggct cagcgcggtg gaaatttccg acgtggacga aaagatgcgc ccgattgcgg
     1741 gaacggaaga gatcatcgaa tgcgacgcgc tgattctctc cgtcggcctg attccggaaa
     1801 acgaacttgc ggaatcgctc ggcgttccgc tggatggcaa gacgaaaggc gcggtcgtgg
     1861 acgaacagaa ccagacgatg ctggacggtg tatttgcctg cggcaacgcg ctgcatgtca
     1921 acgaccttgt cgattacgtt tccgaaagcg gcgaaactgc gggcagagcg gcggcggttc
     1981 acgcaaagga gccggatgcg aaagttcgcg atcttgcgcc ggtgaactgc gacgaaaggc
     2041 tgctatacgt cgtcccgcaa aagctagatc gtcatgc
//
LOCUS       DAOMGY010000295         2074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_60969_length_2074_cov_2.848935, whole genome shotgun sequence.
ACCESSION   DAOMGY010000295 DAOMGY010000000
VERSION     DAOMGY010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60969_length_2074_cov_2.848935"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2074)
                     /locus_tag="PLR57_05225"
     CDS             complement(<1..>2074)
                     /locus_tag="PLR57_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HQQ40892.1"
                     /translation="GVWTDVYALSATIYDLLCGQAPPEAVLRTGRDTLVKPNKLGARL
                     SPRQQRALLRGLTVHVSPRVSSMELLRSKLYNLPLPEEVRWRKRLIRNIAFAGAVIVL
                     VGGLFALNATTGLPTFSGVCFTIGKDGISAARYIGKKSSFSIPDRLLGLDVSEISSQA
                     FAGQDDLVSVHLPGTVHSIGEMAFSGCAKLSALNIPEGTSLIGDYAFLGCKMLSNITL
                     PSSVTSIAENAFEDCSDSLTLYGESGSYAEQYAGENGIKFVVASEFSYYVINGGAVIT
                     SYRGSASDVVVPAELGGCPVRRIDDAVFMFNYDLRSIELPDTLRSIGESAFGNAYHLK
                     SIRLPEGFLTIEGNAFWSCFALESVELPSTLRSIGESAFDMCWSLSSIAIPDGITELK
                     DYTFLDCERLESVAFPSSLKSIGAFAFADCVQLSHLRLPNTIQSIGESAFSGCPALKS
                     LYVPEGVLSIDPSAFGRNEFDKVENTCPNLTIIGYPNTVAQTAASACDLKFDDVTRWE
                     PVRNFLYRYENGGITITWYRGSAVDLVIPSYISGLPVLRIGDQAFDSNVRIQSVALPL
                     RIKQIDANAFSGCSNLKYIELPEGLETIGDHAFFHCALENVVLPDSLTSLGLEGFIDN
                     YYLKTLSVGNGLLSWSGYDFDGADELVSLTIEATSSLGYCGFPSLLSLRLGGGVQRIT
                     SGAFTNCPE"
BASE COUNT          517 a          551 c          589 g          417 t
ORIGIN      
        1 actctggaca gttggtgaac gcgccggacg tgatgcgttg cactccgccg ccaagccgca
       61 aggaaagcag cgagggaaaa ccgcagtacc ctagcgaaga cgttgcttca atagtcaagc
      121 ttaccagctc gtccgcaccg tcaaagtcat aaccgctcca actgagcaga ccgttgccga
      181 cagaaagcgt tttaagatag tagttatcga tgaacccttc caaaccaaga gaggttagac
      241 tgtcaggcag gacgacgttt tccagcgcgc agtggaagaa tgcgtggtcg ccaatcgttt
      301 ccaacccttc gggcagctcg atatacttta aattggaaca accggagaac gcattggcat
      361 cgatctgttt tatacggagt ggaagcgcaa cgctctgaat tctcacattg gagtcaaaag
      421 cttgatcacc gatgcgtaat accggcaggc cgctgatgta ggaaggtata accaaatcga
      481 cggcactgcc gcgataccat gtaatggtaa tgccgccgtt ttcatatcga tataagaaat
      541 ttctgacggg ttcccagcgc gtcacgtcgt cgaacttcaa atcacaggcg gaagccgctg
      601 tctgcgccac ggtattcgga taaccaatga tcgtcaggtt ggggcaagta ttctccactt
      661 tgtcgaattc atttcgacca aaggcggaag gatcaatcga aagaactcct tctggaacat
      721 aaagcgattt taaggccgga caaccggaaa aagcgctttc tccgatcgat tggattgtgt
      781 tcggcaaacg aagatgagaa agctgaacac agtcggcaaa agcgaacgcg ccaatggatt
      841 ttagagaact cgggaaggcg acgctttcga gtcgttcaca atccaaaaag gtgtagtctt
      901 tgagttctgt aattccgtcc gggatggcaa tagagcttaa cgaccagcac atatcgaaag
      961 cggattcccc gattgaccgc agcgtgctgg gcagttcgac gctttcgagt gcaaaacaac
     1021 tccagaaagc attgccctcg atggtcaaaa acccttccgg taagcggatg gacttgaggt
     1081 gataagcgtt gccaaacgcg ctctcaccga tggaacgcag tgtgtccggc agctcgatgg
     1141 agcgcaggtc gtagttgaac ataaacactg cgtcatcaat gcgccgcacg gggcatccgc
     1201 cgagctcggc aggaacgacc acgtcggaag cgctgccgcg ataactcgtt atcacggcgc
     1261 cgccgttgat gacgtagtag ctgaattcgg aggcaacgac gaacttgatg ccgttttcgc
     1321 cggcatactg ctcggcgtaa ctgccgcttt cgccgtagag tgtgagactg tccgaacagt
     1381 cttcaaacgc gttttcggca atgcttgtta cgctggaggg aagcgtgatg tttgagagca
     1441 tcttgcagcc taaaaacgcg tagtccccga tgagacttgt gccttccgga atattcagcg
     1501 cgctgagttt tgcgcagccg gaaaacgcca tttcgccgat ggagtgcacc gtccccggca
     1561 gatgcacgga aacgaggtcg tcctgaccgg caaaagcctg gctggaaatt tcggaaacat
     1621 ccagccccaa caggcgatcc ggtatggaaa agctcgactt tttgccaata tagcgcgcgg
     1681 cggagatgcc gtctttgccg atggtaaaac agacccccga gaacgtcggc agccccgtgg
     1741 ttgcgttgag ggcgaacagc ccgccgacaa gcacgataac tgcgccggcg aacgcgatgt
     1801 tgcggatgag ccgctttctc cagcggactt cctcgggaag cggcaagttg tagagtttgc
     1861 tgcgcagcag ttccatgctg gaaacacgcg gggaaacatg aacggtgagg ccgcgcagca
     1921 gcgcgcgctg ctggcgcggg gaaaggcgcg cgccgagttt gttcggcttg accagcgtgt
     1981 cgcgcccggt gcgcagaacc gcttccggcg gcgcctgacc acagagcaga tcgtaaatag
     2041 ttgccgaaag cgcataaacg tccgtccaga cgcc
//
LOCUS       DAOMGY010000296         2072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_61074_length_2072_cov_2.737234, whole genome shotgun sequence.
ACCESSION   DAOMGY010000296 DAOMGY010000000
VERSION     DAOMGY010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61074_length_2072_cov_2.737234"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLR57_05230"
     CDS             complement(<1..285)
                     /locus_tag="PLR57_05230"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQQ40893.1"
                     /translation="MKKRIACLLLILLALSSAAMPLKDARALSVTTGVIVGDDVALRK
                     TAANNGKLVARLDEGTVVKILETNVFSEWYRIEAGTRTGYVNRMYVNIDPS"
     gene            complement(463..>2072)
                     /locus_tag="PLR57_05235"
     CDS             complement(463..>2072)
                     /locus_tag="PLR57_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HQQ40894.1"
                     /translation="QIYPRSFMDGNGDGIGDIPGIIEKLDYLHELGVTALWLSPIFES
                     PNDDNGYDVSNYRAIMKEFGTMRDVEKLIKELHKRDMRLILDLVVNHTSDEHEWFIDS
                     VKHPEGKHGDYYIWRKPKADGQPPNNWKSFFSGSAWEAVEGREEMYLHLFSKKQPDLN
                     WENPAVRDEVCDIIDFWREKGVDGFRLDVINYISKTSLEDGNETLGSLLGFEGIEHYF
                     YGKRLHEFLHELRTRAFKEAFTVGETPGTGPAMNQLLTADERGELNTVFSFDHLENQG
                     KNRYDVYRYNLNHLKRCFIGYEGAYTDVAWPSIFVENHDNPRMVSKIDPREEYRLAIA
                     KLLAVLLLTARGTVFLYQGQELGAVNVPFTGIGELRDIESLNKYKELVAAGATPQTAF
                     QTVLTGTRDHARTPMQWTSGRYGGFSEHEPWICVGDKDECNADAQMQDETSAWSAYQS
                     LITLRREHPALVYGKFEVVEEKKEDVFCYFRDDGLERFYIEVNLCSRQIARPKQRGTM
                     QLIFSNYMASSDGMQPYEANVYCVDRV"
BASE COUNT          408 a          589 c          592 g          483 t
ORIGIN      
        1 gctgggatcg atattgacgt acatgcggtt gacgtagccc gtgcgggtgc ccgcttctat
       61 gcggtaccat tcggaaaaca cattcgtttc cagaatctta acaaccgtgc cttcgtccag
      121 ccgcgcgacc agcttgccgt tgttggcggc ggtttttctg agcgcgacat catcgccaac
      181 gatcacgccg gtggtcacgc tgagcgcgcg cgcatccttt aagggcattg cggcgctgga
      241 aagcgcaagc agaatcagca gcagacaggc aatgcgtttt ttcatatgta accttgtact
      301 ccttttgcgt gccgcgcact gcggcagcat ccaagatgag tgtagtgaaa attagacggg
      361 atgacaagcc gcatcccgcc caattcacga aaacgttaca ttagcgttcc cccgcgcgca
      421 gaacgggtca agcccggttc aacacgacag gtaaagcgcg ttttacacac ggtctacgca
      481 gtagacgttc gcttcgtaag gctgcatgcc gtccgacgaa gccatgtagt tggagaagat
      541 cagctgcatc gtcccccgct gttttggccg cgcaatctgg cgcgaacaga gatttacttc
      601 aatatagaac cgctccagcc cgtcatcacg gaagtagcag aacacgtcct ccttcttctc
      661 ctccacgact tcgaacttgc cgtagaccag cgccggatgt tcgcgtcgga gcgtgatgag
      721 cgactgatat gcgctccagg cggaggtttc gtcctgcatc tgtgcgtcgg cgttgcactc
      781 gtccttatcg ccaacgcaga tccatggctc atgctcggag aaaccgccgt aacggccgct
      841 cgtccattgc ataggcgttc tggcatggtc gcgcgtgccg gtaagcacgg tttggaacgc
      901 ggtctgcggg gttgcgcctg cggcaaccag ctctttgtac ttattcaggc tctcgatgtc
      961 tcgcagttcg ccgatgcccg taaacggaac gttcaccgcg cccagttcct gcccctgata
     1021 gagaaacacc gttccgcgcg cggtaagcag cagaaccgct agcagtttgg caatggcaag
     1081 gcggtattct tcgcgcgggt cgatctttga gaccatgcgg ggattgtcgt ggttttccac
     1141 aaagatactc ggccatgcaa cgtccgtata cgccccctcg tagccgatga agcagcgctt
     1201 gaggtggttg aggttgtagc gataaacgtc gtaacggttc ttgccctgat tttccagatg
     1261 atcgaagctg aacacggtgt taagttcgcc gcgctcgtcc gccgtaagga gctggttcat
     1321 ggcggggccg gtgcccggcg tttcgcctac tgtgaacgct tccttgaagg cgcgcgtacg
     1381 gagctcatgt aaaaactcgt gcaggcgctt gccgtaaaag taatgctcga ttccctcgaa
     1441 ccccaataag gagccgagcg tctcgttgcc gtcctcgaga gaagtcttgc tgatgtagtt
     1501 gattacgtcc agacgaaagc cgtccacgcc tttttcacgc cagaagtcga tgatgtcgca
     1561 gacttcgtcc cgcacggcgg gattctccca gttcaggtcg ggctgcttct tggaaaacag
     1621 gtggagatac atctcctcgc ggccttccac cgcttcccat gcgctgccgg agaagaagga
     1681 tttccagttg ttcggtggct gaccgtccgc tttaggcttg cgccagatat agtagtcccc
     1741 gtgcttgcct tcggggtgct tgaccgagtc gatgaaccac tcgtgttcgt cgctggtgtg
     1801 gttgaccacc aaatcgagaa tcaggcgcat gtcgcgcttg tggagttctt tgatgagctt
     1861 ttcgacgtcc cgcatggtac cgaattcctt catgatggcg cggtagttgc tcacgtcgta
     1921 gccgttgtcg tcgttgggcg attcgaaaat gggcgaaagc cagagcgcag tcacaccgag
     1981 ctcgtgcagg tagtcgagct tttcgatgat acccggaatg tcgccaatgc cgtcgccgtt
     2041 gccgtccatg aacgagcgcg ggtatatctg gt
//
LOCUS       DAOMGY010000297         2071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_61123_length_2071_cov_2.777282, whole genome shotgun sequence.
ACCESSION   DAOMGY010000297 DAOMGY010000000
VERSION     DAOMGY010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61123_length_2071_cov_2.777282"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLR57_05240"
     CDS             <1..613
                     /locus_tag="PLR57_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HQQ40895.1"
                     /translation="VDGLYGAMASLSGSGVSLEWYKSKFLPGVDYRTLDNEAEAREIG
                     DLFFYPYPLGAAYPLWNSNARAAFTGVTLEHDPYAFTRAILEGVAFGVRRTLADFAEN
                     GCAVKSLKVIGGAAKSALWTSLICDITNVPLELMQESDACALGAAVIAAVGAGAFAGY
                     RAATDAMVHRARIQQPEPERAAYYAAKYGRFCSLWDHMSPYYR"
     gene            968..1996
                     /locus_tag="PLR57_05245"
     CDS             968..1996
                     /locus_tag="PLR57_05245"
                     /inference="COORDINATES: protein motif:HMM:NF037759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40896.1"
                     /translation="MKNKKAMTYLLTIGMLLLALAVLSGPRPLPKEIISAQAQVNGSV
                     DAETSNGSEEIAANAVPLAGPSDIAPQTIQLVARQEPVKDEPATAEPIIEEPAPEEPV
                     KEEPVAEEPAPEDPVEEEPAPEDPVEEEPAPEEPTEEEPVEEEPAPEEPAEEEPPIVT
                     LTAAQKLLERSYALLNQYNACTTMEEKRVLLGASNYSLGNDALRAKLLADMGGSWELL
                     EEEVTDATEYQQEKTLYVQVYMSGSSSDFTPVVYTAPNSDRSGNQWATNLVYVENEAT
                     WMEYVKKHPYNDSRVTYYVTGLAPEGSWDTLQETMETSDLWQEVELEEEAPADDSAPL
                     AETDPTLG"
BASE COUNT          476 a          594 c          598 g          403 t
ORIGIN      
        1 cgtggacggg ctttatggcg caatggcttc gctctccggc tccggcgtgt cgctggaatg
       61 gtataaatcc aagttcctgc ccggcgtgga ttaccgcacg ctcgataacg aggcggaagc
      121 gcgcgaaatc ggagatctgt tcttttaccc gtacccgctc ggcgcagctt acccgctctg
      181 gaactccaac gcccgcgcgg cctttacggg ggtcacgctg gagcacgacc cctacgcttt
      241 tacgcgcgcc atcttagaag gcgtcgcgtt cggcgtgcgc aggacgctcg cggactttgc
      301 ggaaaacggc tgcgcggtca agtccctcaa ggtcatcggc ggcgcggcaa agagcgcgct
      361 ctggacgagc ctgatctgcg acattacgaa cgttccgctg gagctcatgc aggagtccga
      421 cgcctgcgcg ctgggcgccg ctgtcatagc agccgtcggc gcgggtgcgt ttgcgggtta
      481 ccgcgcggcg acggacgcga tggttcaccg cgcgcgcatt caacagcccg agcccgaacg
      541 cgccgcttat tacgcggcaa agtacggcag gttctgctcg ctttgggatc acatgtcccc
      601 ctattaccgc tgaaccgcac gttttccaaa cgccgcccgt cagggcggct ttttttgctt
      661 ctcgaatgcc gaaagacagg cagacatccg gctggtatgt tcgcgcgtaa aagcgtgcgt
      721 tttcgaaccg ctgttgcttg ctttgcgctg tacttgcgca ttttcagttt tctaagtaac
      781 gcttatgtaa catcgcgctc gtgattttgc gttttaacgg cgatttcggg tgataaacgc
      841 acgtaaaaaa gcaatttgtg atgcaaaaat atatttttgt ttctttttta agtctttgaa
      901 atgaaacgga tgtgtaacaa aatgcgcgta gactattgtt tgtaaggata aagaaggagc
      961 aaacaagatg aagaataaga aagccatgac atacctgctg accatcggca tgctgctgct
     1021 ggcgctggcg gtgctgtccg gcccgcggcc gctcccgaag gaaatcatct ctgctcaggc
     1081 gcaggttaac ggctcggtcg atgcggaaac atcgaatggt tcggaagaga ttgccgcgaa
     1141 cgcagtgccg ctcgcaggcc cgtccgacat tgcgccgcag acgatacagc tcgtcgcgcg
     1201 gcaggaaccc gtcaaagatg aacccgcaac ggcggaaccc atcatcgaag aacccgcgcc
     1261 ggaagaaccc gtcaaggaag aacctgttgc agaagaaccc gcgcccgaag atcccgtcga
     1321 ggaagagccg gcacccgaag accccgtcga ggaagaacca gcacccgaag aacccacgga
     1381 agaagaaccc gtcgaggaag agccagcgcc tgaggaacct gcggaagaag aacccccgat
     1441 cgtgacgctg actgccgcac agaagctgct ggaacgctcg tatgcgctcc tgaatcagta
     1501 caacgcctgc accaccatgg aagaaaagcg cgtactgctg ggagcaagca actactcgct
     1561 gggcaacgac gcgctgcgcg caaagctcct tgccgacatg ggcggcagct gggaactgct
     1621 ggaagaagaa gtgacggacg caacggaata tcagcaggaa aaaacgctgt atgtgcaggt
     1681 ttatatgtcc ggttcatcca gcgactttac ccccgttgtc tacaccgcgc cgaattccga
     1741 ccgaagcggc aaccagtggg cgacgaacct tgtttacgtc gaaaatgaag cgacctggat
     1801 ggagtatgtt aagaagcatc cgtacaacga ttcccgcgta acatattatg tcacagggct
     1861 cgcgcccgaa ggttcctggg atacgttgca ggaaacgatg gaaaccagcg acctctggca
     1921 ggaagtggag ctcgaagaag aagcccccgc agatgattcc gcgccgcttg cggaaaccga
     1981 tcccacgctg ggataaatat cggcgaactt ctaatccggc gcagccatcc ccggctgcgc
     2041 tgtttttcta tcttcatgtt cggcctgtcc g
//
LOCUS       DAOMGY010000298         2071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_61130_length_2071_cov_2.294643, whole genome shotgun sequence.
ACCESSION   DAOMGY010000298 DAOMGY010000000
VERSION     DAOMGY010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61130_length_2071_cov_2.294643"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..1104
                     /locus_tag="PLR57_05250"
     CDS             16..1104
                     /locus_tag="PLR57_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40897.1"
                     /translation="MEESQQTGRTAKPLPKRIGRALIWFFIAMLALTFISRAASDALK
                     AKVSIGYLSSSSLDQSLTGTGGWVSGNTQFYTTYYTRRISKVYVQPGQQVKEGDPLFA
                     YDVATVSGGKTVSDRKVTAAEKALEQAQKNLETAEDKEYAVRVVESAAQALDYARFTF
                     AQTSALQNGGVVRATFGGTILSCDLTPGKASTAGISGLEVALGDALFQFTVSAKQAER
                     ISLGDSVLIYRDSKQEKQPLEVVSLSTPDADGKVTVRCGGGGEGRAIGSEQDWKLKKQ
                     SGQYDTCVPLEALRQGGSNDYYVLVLQEKQTILGTQLMAKRVSVELLAHDGSHAAVEG
                     ELTREDKLITACSKEIKDGDLVVKNDAD"
     gene            1091..>2071
                     /locus_tag="PLR57_05255"
     CDS             1091..>2071
                     /locus_tag="PLR57_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40898.1"
                     /translation="MTRTKRGLWRAVLLGLIAAGCWLAALIIAGDLAEQYGGLSVRFA
                     KAAVTQKDLERAQSESTSEELVLRAAWTRKKELQTLGSELGGSAKARVIGIYGDPRQI
                     APLSLLSGGFLPENDEEGCVLDAVSAWALFHSSEAVGATLNLNGRVYVVRGIVKSYEP
                     MLIIRDDTLKYENLEFGVSDPAVGKQEAETFFARCGAGGEYLVVQSGLYVRLILGFVC
                     IAAGGFLLAGAAALLKSAWLKKDYSCARLFFLTAGIALAAAAVLLFIKTFYWPQSFLP
                     TRWSDFAFWRGLIESWQADWKAISLMTPLPKEIQLFRSLRCCFVLLLVSVL"
BASE COUNT          449 a          515 c          654 g          453 t
ORIGIN      
        1 tcaaggagta gtgttatgga agagagccaa cagaccggac ggacggcaaa accgctgcca
       61 aagcggatcg gccgcgcact catctggttt tttatagcga tgctggcgct gacgtttatt
      121 tcccgtgcgg cgagcgacgc gctcaaggcg aaggtttcga ttggctattt gagttcgtct
      181 tctctggatc agagcctgac gggcacgggc ggctgggttt ccggcaatac gcagttttac
      241 accacgtatt atacccgtcg tatttcaaag gtctatgttc agcccggcca gcaggtgaag
      301 gaaggcgacc cgctgtttgc ctacgacgtc gctaccgttt ccggcggcaa gactgtttcc
      361 gacagaaagg tgaccgcggc ggaaaaggcg ctcgaacagg cgcagaagaa cctggaaacc
      421 gccgaggaca aggaatacgc cgtgcgcgtt gtggaaagcg ctgcgcaggc gctcgactac
      481 gcccgcttta cctttgcgca gaccagcgcg ctacaaaacg gcggtgtagt acgcgcaacg
      541 ttcggcggaa cgattctgag ttgcgacctt acgccgggca aggcttccac cgccggaatc
      601 agcgggctgg aagtcgcttt gggggacgcg ctgtttcaat ttactgtgag cgcaaagcaa
      661 gcggagcgca tttcgcttgg cgattcagtt cttatttatc gtgacagcaa gcaggaaaag
      721 caaccgctgg aggtggtttc tctctcaacg ccggatgcag acggcaaggt aaccgttcgc
      781 tgcggcggcg gaggggaagg gcgcgcaatc ggttcggagc aggattggaa gctcaaaaag
      841 caatccggcc agtacgatac atgcgtgccg ctggaggcgc tgcgtcaggg agggagcaac
      901 gactattatg ttctggtgct gcaggaaaag cagacgattc tcggcacgca gcttatggcg
      961 aaacgcgtga gcgtggaact gctcgcacat gacggcagcc acgcagccgt ggaaggcgaa
     1021 cttacgcgcg aggacaagtt gattaccgcc tgttccaagg agatcaaaga cggtgatttg
     1081 gtggtgaaaa atgacgcgga ctaaacgcgg gctttggcga gcggttctgc tcggtctgat
     1141 cgctgcagga tgctggctgg cagctttgat catagcgggc gatctcgcgg agcagtacgg
     1201 cgggcttagt gtgcgctttg ctaaagccgc ggtcacacaa aaggatctgg agcgggcgca
     1261 aagtgagtct actagtgaag aacttgtact gcgagccgcc tggacgcgaa aaaaagagct
     1321 ccaaacgctt ggttcggaac tcggtggaag cgcaaaagcg cgcgtgatcg gcatatatgg
     1381 cgatccgcgg caaatagcgc ccctttcgct gctttccggt gggtttttac cggaaaatga
     1441 cgaggaggga tgcgttctcg acgcggtttc cgcctgggcg ttgtttcatt cctcggaagc
     1501 cgtaggcgca acactcaacc tgaacggcag ggtatacgtc gttcgcggca tagtaaaaag
     1561 ctatgaaccg atgctgatta ttcgtgatga tactctgaaa tacgagaacc tggaattcgg
     1621 tgtgagtgac cccgccgtgg ggaaacagga agcagaaacc ttttttgcgc ggtgcggagc
     1681 cggcggagag tatctggttg tgcaaagcgg cctgtatgta cgtttgatac tggggtttgt
     1741 ctgcattgcg gcaggcggct tcctgcttgc gggggcagct gctctgctga aaagcgcatg
     1801 gctgaaaaag gactattcat gcgcccgctt attctttctg acggcaggaa tcgcgctggc
     1861 tgcagcggca gtgctgctgt ttataaaaac cttctattgg ccgcaatcgt ttctgccaac
     1921 cagatggtcg gactttgcct tctggcgtgg tttgatcgaa agctggcagg cggactggaa
     1981 agcaatctct cttatgacgc cgcttcccaa ggagatacag ttgtttcgct ctctacgctg
     2041 ctgtttcgtt ctgctgcttg tttcggttct g
//
LOCUS       DAOMGY010000299         2070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_61159_length_2070_cov_3.707692, whole genome shotgun sequence.
ACCESSION   DAOMGY010000299 DAOMGY010000000
VERSION     DAOMGY010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61159_length_2070_cov_3.707692"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="PLR57_05260"
     CDS             <1..893
                     /locus_tag="PLR57_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40899.1"
                     /translation="PGDTRTLENWSSAEESENAGGGHVEIGADSDPITGLFALSNQLL
                     IFKRDSLYRLLGDRPSNYRILPVSGSVQLPCHTACARSGDVVYFLTENGMYYYDGQTV
                     CRKGDAEKASAFIKTADLAVCDAAACRDKLYFAVTENAGSGANDAMLVYDLSRGTYMA
                     RRGFLARGLCVCGGVLYLMDGAGCVCRFDEGETYGGARIDAYWKTPMTDLDTKAVNKH
                     LEELFLRGTGGILSLEAVTEGGTVYNERLMPGSGENILELHLTGDGRAFQLVFRNVNG
                     SYFSVDGGVELLMDLQRRII"
     gene            913..1101
                     /locus_tag="PLR57_05265"
     CDS             913..1101
                     /locus_tag="PLR57_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40900.1"
                     /translation="MAYESTGMQALFPVYLSRLAEKGASREDYDLSAAQNEGNLNQNF
                     ETLVQKLQEIEDYLSSLT"
     gene            1116..1901
                     /locus_tag="PLR57_05270"
     CDS             1116..1901
                     /locus_tag="PLR57_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40901.1"
                     /translation="MAIQLTKKQAAGSKLDMGLRKLKLGFDEPDAYEDSGSASAASDE
                     DVLSSYIDSMYARYQPQELEFDEQTQDELSASVAAWLRPGYDQAIQNRKAQTQTYRAE
                     IDADAIARGMGSSTYVSDVKSRQNNAEAGDIASLESDYGATLAKYVSQGLETQNEQKL
                     ETASYNAEQRKNAYDMAYAAALQLFAQYKKSGGGSGKKTSVKVSATSRDNCAEFLNQL
                     TKDERAEIYAGTTEQGAQYRAEILASVGYTGYLQLQSQYPSIP"
BASE COUNT          492 a          586 c          636 g          356 t
ORIGIN      
        1 cgccgggcga cacgcgcacg ctggagaact ggtcgagcgc ggaggagagc gaaaacgcag
       61 gcggcggaca cgtggaaatc ggcgcggatt ccgacccgat tacagggttg ttcgcgctga
      121 gtaaccagct tttgatcttc aagcgtgact cgctttaccg cctgctgggc gatcgtccaa
      181 gcaactaccg gatccttccg gtcagcggtt cggtgcagct gccctgccac accgcctgcg
      241 cccgcagcgg ggacgtcgtg tactttttga ccgaaaacgg aatgtactat tacgacggtc
      301 agaccgtatg ccgaaaaggg gacgctgaaa aggcgtccgc gtttattaag accgccgatc
      361 tcgcggtctg cgacgcggcg gcctgccggg acaagctcta ctttgccgtt acggaaaacg
      421 ccggctccgg cgcgaacgac gcgatgctgg tttacgacct ctcaaggggg acgtacatgg
      481 cgcgcagggg atttcttgcg cgcgggctct gcgtttgcgg gggcgtgctg tatctgatgg
      541 acggcgcagg ctgtgtctgc cgctttgacg aaggcgaaac ctacggaggc gcgcgaattg
      601 acgcctactg gaaaacgccc atgaccgacc tcgacacgaa agccgtcaac aagcatctcg
      661 aagagctgtt cttgcgcggc acgggcggca tcctctcgct ggaggcggtg acggagggcg
      721 gaacggtcta caacgaacgg ctcatgcccg gctccggcga gaacattctg gaacttcacc
      781 tcaccggcga cgggcgcgcc ttccaactgg tctttcgaaa cgtcaacggt tcgtactttt
      841 ccgtcgacgg cggcgtggaa ctgctgatgg atctccagcg gcgaatcatt taaggaaagg
      901 aacggcagaa caatggcata cgaaagcaca gggatgcagg cgctgtttcc cgtctactta
      961 tcccgcttgg cggaaaaggg cgcttcgcgc gaggactatg acctctccgc ggcgcagaac
     1021 gaggggaacc tgaaccagaa tttcgaaaca ctggttcaaa aactgcagga aatcgaagat
     1081 tacctctctt cgctgacctg acgaaaggag aacacatggc catccaatta acaaagaaac
     1141 aggcggcggg gagcaaactt gacatggggc tccgcaagct caagctaggt tttgacgagc
     1201 cggacgccta cgaagattcc ggctctgcgt ccgccgcaag cgacgaggac gttctcagct
     1261 cgtatatcga cagcatgtac gcgcgctacc agccgcaaga actcgaattt gacgaacaga
     1321 cgcaagacga actgagcgct tccgtcgcgg cctggctgcg gcccggatac gatcaggcga
     1381 tccagaaccg caaggcacag acgcagacat accgcgcgga gatcgacgcg gacgccattg
     1441 cgcgcggcat ggggtcgagc acctacgttt ccgacgtcaa gagccgccag aacaacgccg
     1501 aagcggggga cattgcgtcg ctggaatcgg actacggcgc gacgctggca aaatacgttt
     1561 cccaaggttt ggaaacgcag aacgagcaga agctcgaaac ggccagctat aatgccgaac
     1621 agcgtaagaa cgcctacgac atggcgtacg ccgcggcgct gcagctcttt gcgcagtaca
     1681 aaaagagcgg cgggggttcg ggcaaaaaga cgtccgtcaa ggtttcggcg acctcgcgcg
     1741 acaactgcgc ggagttcctc aaccagctca cgaaagacga gcgcgcggaa atctacgctg
     1801 gaacgacgga gcagggcgcg cagtaccgcg cggaaatcct cgcttcggtc ggatatacgg
     1861 ggtatctgca gctgcagagc cagtatccga gtattccctg attttgcggc gcaggacggc
     1921 ccgacgagaa tatcgccggg ctgttttcgt ttgtacgctg aagccgaaat caaacgatta
     1981 atcaggaaat atgcaataga ttaagcaaac ttgaaaaaag cgcgtaatgc gcaacgtttc
     2041 atcaggaacc cgtcaagcgg ggttgaagca
//
LOCUS       DAOMGY010000300         2070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_61174_length_2070_cov_2.503722, whole genome shotgun sequence.
ACCESSION   DAOMGY010000300 DAOMGY010000000
VERSION     DAOMGY010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61174_length_2070_cov_2.503722"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLR57_05275"
     CDS             <1..387
                     /locus_tag="PLR57_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HQQ40902.1"
                     /translation="ASVCEESHDDYGPIWPISLAPWEAQICVLRADQEEPKRVGEELY
                     AALTKAGVEAIIDDRPVSAGVVFSEADLIGAPIRLTVSPRNLADGMIELTTRDKSVKR
                     QVSVAETLTAVLALKQELFDRIEGTL"
     gene            444..1460
                     /locus_tag="PLR57_05280"
     CDS             444..1460
                     /locus_tag="PLR57_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQQ40903.1"
                     /translation="MCAAIALFSAGCSIPQFIGHQRGSHAAPAVAAPEDVPVSTPVPT
                     PTPSPSPTPDPAMAPVTYAWISDTQGYASSFPDTFFAMTQWIVDHKVEWNIQYVLHTG
                     DVVNDMRREKEWNVATKAMDVFVGELPVFAIAGNHDIRGVMHDYSGFTPLMERQNYAS
                     YPTFGDEEAGGRRRYDLVNIGYDPFLLIGVGYSLTPRDVKWLDDTLSRYADRTAIVLT
                     HHYLAIEKVPMEQDAELMRGVIARHPNVRYVLCGHMHALQRETQFFDDNGDGTPERGV
                     QAIMADYQGFDHGGEGYIVLLTFDPEQREIRVTSYSPVHDDYNFYGDASQETYTLPLD
                     VVGN"
     gene            complement(1530..>2070)
                     /locus_tag="PLR57_05285"
     CDS             complement(1530..>2070)
                     /locus_tag="PLR57_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQQ40904.1"
                     /translation="NRDVGWLSVNISSQQNEFDETVRRLETLLEKYDIPPECIKLEIT
                     ESVLLEDLERARTTIEELGRRGVGVFLDDFGTGYSNLRNVMSLPFECIKIDKGLLGGI
                     HQNEKAYRLLQTVVNGLRSMNMLLLAEGVETLEQSETVRHLGIDLIQGYYYARPMPSE
                     EFVWQVKKVPESVMNAELT"
BASE COUNT          424 a          641 c          574 g          431 t
ORIGIN      
        1 gcgagcgtct gcgaagaaag tcacgacgac tacggcccca tctggccgat ctcgctcgcg
       61 ccctgggaag cgcagatctg cgtgctccgc gccgatcagg aagagccaaa gcgcgtcggc
      121 gaagagctct atgccgcgct cacgaaggcc ggcgtagaag cgattatcga cgaccgtccg
      181 gtcagcgcag gtgtggtctt ttcggaagcc gacctcatcg gcgcgcctat ccgcctgacc
      241 gtcagccccc gcaaccttgc ggatggtatg atcgagctca ctacgcgcga taagagcgtc
      301 aagcgtcagg tgtccgttgc cgaaacgctc accgccgtgc tcgcgctcaa gcaggaactc
      361 tttgaccgta tcgagggtac gctgtgataa aatcacggtc aaaccaaccg aacagcaatc
      421 acgcatggct gcgcgcactt tccgtgtgcg cggccattgc gctttttagt gcaggttgtt
      481 ccattcctca gtttattggt catcagcgcg gctcacatgc cgcacctgcg gttgcagcgc
      541 cggaggatgt tcccgtttcc acccccgttc ccacgccaac gccgtcccct tcccccacgc
      601 ccgaccctgc catggctccc gttacctacg cctggatcag cgacacgcaa gggtacgctt
      661 ccagctttcc ggatacgttt ttcgccatga cgcaatggat cgtcgaccac aaagtggaat
      721 ggaacattca gtatgtgctc cacaccgggg acgttgtcaa cgacatgcgc cgtgaaaaag
      781 aatggaacgt ggcaaccaag gcgatggatg tgttcgttgg cgagctgccc gtattcgcca
      841 tcgcgggcaa ccacgatatc cgcggcgtaa tgcacgacta cagcggattt acgcctctga
      901 tggagcgcca gaactacgcc tcttatccga catttggcga cgaggaagcg ggcgggcgca
      961 ggcgctacga cctcgtaaac atcgggtacg acccgtttct gctcatcggc gtcgggtatt
     1021 cgctcacgcc cagagacgtc aagtggctcg acgatacgtt gagccggtac gccgaccgca
     1081 ccgccatcgt tttgacgcac cactacctcg ccatcgaaaa agtccccatg gagcaggacg
     1141 cggagctgat gcgcggcgtc atcgcccggc atccgaacgt aagatacgtg ctctgcgggc
     1201 atatgcacgc actccagcgc gagacgcaat tttttgacga caacggagac ggaaccccgg
     1261 agcgcggcgt gcaggcgatt atggcggact atcagggttt tgaccacggc ggagaaggct
     1321 acatcgtcct gttgacgttc gacccggaac agcgggaaat ccgcgtcacg tcgtattccc
     1381 ccgtccacga cgactacaac ttttacggcg atgcgtcgca ggaaacctat accctgccgc
     1441 tcgacgtcgt tggtaactga cagtaaaaac gctggataca caaaacgccg cgaacctgtt
     1501 gttcgcggcg tttctgtttg tttcattggt taagtgagtt ctgcgttcat caccgactcc
     1561 ggaaccttct tgacctgcca gacgaactcc tcgctgggca tcgggcgggc gtagtagtaa
     1621 ccctgtatca ggtcgattcc cagatgccgc accgtttcgc tctgctcgag cgtttctaca
     1681 ccttccgcca gcagcagcat gttcatggag cggaggccgt taacgaccgt ctgcagcagc
     1741 cggtaggctt tttcgttctg gtggatgccg ccgagcaggc ctttgtcgat tttgatacac
     1801 tcgaacggaa gtgacatgac gtttctaagg ttggagtagc ccgtgccgaa gtcgtccaga
     1861 aataccccga cccctctgcg gccaagttct tctatcgtgg tgcgcgcgcg ctccaaatcc
     1921 tcaagcagca cgctctcggt aatctccagc ttgatgcact ccggcgggat atcgtatttt
     1981 tccagcaggg tttcgagcct gcgaaccgtt tcgtcaaact cgttctgctg agaagaaatg
     2041 ttgaccgaaa gccagccgac atcccggttc
//
LOCUS       DAOMGY010000301         2069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_61223_length_2069_cov_3.138530, whole genome shotgun sequence.
ACCESSION   DAOMGY010000301 DAOMGY010000000
VERSION     DAOMGY010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61223_length_2069_cov_3.138530"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1586
                     /locus_tag="PLR57_05290"
     CDS             66..1586
                     /locus_tag="PLR57_05290"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQQ40905.1"
                     /translation="MQQNDNILEVCGISKSFGAVHALKDVSFNIRRGEIHTLLGENGA
                     GKSTLIKVLSGEHAPDSGSILIDGEPVKSFHPLASQAAGITVVHQELSIFENLTVYEN
                     IFPYREGKHRLIPKKQLIEKVKQAAARFDMEIDPEAKMGDLRLSVQQMVEILRALSEN
                     AKIVLLDEPTSGLNTQETENLMRTLKKLRDGGITIIYISHRISEIMEISDRVTVLRDG
                     MYINTFVNDANLTENMLISSMVGRDFEKSIYSKKRSTVPADAEIVFDAQNFTKGKTVV
                     NASLGVRRGEIMGVFGLEGSGTYEFSRMMFGLHGKDTGRILFEGKEIKPITPTVMIEK
                     GIIFLNNNRKDAGLLFNMSIADNMAIPVIDRLTKGALLSRKAMENYTLSFVSQFSIVL
                     NSIYDKPSSLSGGNQQKVMLSVCLGTKPKFLIVNEPTRGIDVGAKLEILRLLNEIVNT
                     GVSVLCFSSDLPELITLSDRIAVMNGGRIAGVIDCDDISENSVMQLAAVDVVEKGA"
     gene            1593..>2069
                     /locus_tag="PLR57_05295"
     CDS             1593..>2069
                     /locus_tag="PLR57_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40906.1"
                     /translation="MEAINTSRLTHRKFRLGEYTILFLIFALFLLLTLTSDTFCTYNN
                     LYSLLYGVSIQFFAVIGFTLLIIMGEIDLAVGSMYGLSGTLVGFCMTVLKWKLLPSSI
                     VVLIVCAAFGWCIGALVTRFKLNSMMVTLGSLSFVSGVNAVVFNSFTAVTYNAEYRS"
BASE COUNT          466 a          556 c          567 g          480 t
ORIGIN      
        1 cagaatggct gccctcggag tttggcttcg ggggcagcca tattcttatg taaggagttt
       61 tttccatgca gcagaacgac aatattttgg aagtgtgcgg catttccaag agctttggag
      121 cggtgcatgc gctcaaggac gtcagcttca atatccgccg cggcgaaatc cataccctgc
      181 tcggcgaaaa cggcgcgggc aaatccacac tcatcaaggt gctctccggc gaacacgcgc
      241 cggacagcgg ctcgattctg atcgacggcg aaccggtcaa gtcgttccat cctcttgcgt
      301 cgcaggcggc ggggattacg gtcgtccatc aggagctttc gatcttcgaa aaccttaccg
      361 tatacgaaaa catctttccc taccgggaag gcaagcatag attgatcccg aaaaagcagc
      421 tgatcgagaa agtaaagcag gcggccgccc gcttcgacat ggagatcgac ccggaagcga
      481 agatggggga cctgcgtctc tccgtccagc agatggtcga gattctgcgc gcgctctccg
      541 aaaacgccaa gatcgttctg ctggacgagc cgacatccgg cctgaatacg caggaaacgg
      601 aaaatctcat gcgcacgctc aaaaaactgc gcgacggcgg gattacgatt atctatatct
      661 cccaccggat ctcggagatt atggagattt ccgaccgcgt aacggttctg cgggacggca
      721 tgtacatcaa caccttcgtc aacgacgcaa accttacgga aaacatgctc atcagcagca
      781 tggtcgggcg cgattttgaa aagagcatct attccaaaaa gcgttcaacc gttcccgcgg
      841 atgcggagat tgtgttcgac gcgcagaatt tcaccaaggg caaaacggtg gtgaacgctt
      901 ctctcggcgt acggcgcggg gagattatgg gcgtgttcgg tctggaaggc tccggcacct
      961 acgagttttc gcgcatgatg tttggcctgc acggcaagga caccggcaga attctctttg
     1021 aaggcaagga gatcaagccc attacgccga ccgtgatgat tgaaaaaggt atcatctttt
     1081 taaacaacaa ccgcaaggac gcggggcttt tgtttaacat gtccattgcg gacaacatgg
     1141 cgattcccgt tatcgaccgg ctgacaaaag gcgcacttct ctcccgtaag gcgatggaga
     1201 actacacgct ttcgtttgtg agccagtttt ccattgttct gaactcaatt tacgacaagc
     1261 ccagcagtct ttccggcgga aaccagcaga aggtcatgct ttctgtctgc ctcggcacga
     1321 aaccgaagtt cctgattgtc aacgagccga cgcgcggcat agacgttggc gctaagctgg
     1381 agatactccg cctgctcaac gagatagtca acaccggcgt ttccgtgctc tgctttagtt
     1441 cggacctgcc ggagctcatt acgctttccg accggattgc cgtgatgaac gggggacgga
     1501 ttgcgggcgt gatcgactgc gacgatatca gcgaaaacag cgtgatgcag ctcgccgccg
     1561 tggacgttgt ggaaaaagga gcgtaaccgg caatggaagc tatcaataca agtcgtttga
     1621 cacacagaaa attccgtctg ggcgaataca cgatcctctt tttgatcttc gcgttgtttt
     1681 tgctgcttac gctgacttcg gacacgttct gcacctacaa caacctttat tctctgctct
     1741 acggcgtttc cattcagttt ttcgcggtga tcggctttac gctgttgatc atcatgggag
     1801 aaatcgatct tgcggttggc tccatgtacg gcctgtccgg cacgctggtc ggcttttgca
     1861 tgacggtttt aaaatggaaa ctgctgcctt cgtcgattgt cgtgctgatc gtctgcgcgg
     1921 cgttcggctg gtgcatcggc gcgctggtga cgcgctttaa gctcaactcc atgatggtga
     1981 cgctcggctc gctctcgttc gtctccggcg tgaacgcggt ggtgttcaac agctttacag
     2041 cggtcaccta caacgcagag taccgctcg
//
LOCUS       DAOMGY010000302         2069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_61229_length_2069_cov_2.754220, whole genome shotgun sequence.
ACCESSION   DAOMGY010000302 DAOMGY010000000
VERSION     DAOMGY010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61229_length_2069_cov_2.754220"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1642)
                     /locus_tag="PLR57_05300"
     CDS             complement(233..1642)
                     /locus_tag="PLR57_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQQ40907.1"
                     /translation="MRLKTKVMAVSVTLLACAITVCSLFILRFARQNVMDSITAAALS
                     DYASFYDQYADKASVGNGASKRTLQTVLKYQLAAMKGNEEYTLQRGDDMLANGIGIDA
                     PATASLGGAKQYVFESFEMSVQYTTAKSGGETYFLVVGELTGAQTGYTLSLVRNITSQ
                     SEALSMLAVKCALAGTAITLFAAMLMLLAMQYFLKPIQSLKAAASELARGHYENRIVC
                     SGKDELAELADNFNSMAGAIQTSMRELQRKAEQQQAFINDLSHELKTPVTSILLCSET
                     LLSRSVPQEAQTRSLERIRHQSRWLEKLSQKLMTLVLLQGEITLRPEDVSALLDAVRE
                     TTADSLEQKGVTLSAECKMERTAMDFDLMRSALANLVENAKNASEPGQQIELSAYGGR
                     FEVTDHGKGIPPEEIARITEPFYRIDRSRSKLSGGSGLGLALVKRIAEAHGARMEIES
                     TLGEGTTMRLVFPQEMTTV"
     gene            complement(1645..>2069)
                     /locus_tag="PLR57_05305"
     CDS             complement(1645..>2069)
                     /locus_tag="PLR57_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQQ40908.1"
                     /translation="TARADLPSRVRGLRGGAEDYMIKPFEILELLVRMEKILDRAGKL
                     NKVLRYRDITIDTEKRTVVQCGTAVPLQPLEFDLLVKLVRFKNCTLPRERLLNEIWGI
                     DFAGGTRTVDIHVAHLRKKLHLEDAILPVSKVGYRLED"
BASE COUNT          404 a          616 c          539 g          510 t
ORIGIN      
        1 ctgcgtttgt tccgtgcccg aagcggatac ctgtacggtc tgtgcttctt caatcaggcg
       61 ctcgccgtcc tttgcaaccg taaccgctgc ttttgtgcct gtgcaggcgc aaagcagcat
      121 tgcggctgcc agtaaaaata gaatactgaa tctctttttc atatcgttac ccctttcgtg
      181 gaataagggt attctgccat tgatttcgaa caaagtcgtt gccgtactgt tatcaaactg
      241 ttgtcatttc ctgcggaaag accagccgca tcgtcgtacc ctcgcccagc gtgctttcaa
      301 tttccatgcg cgcgccgtgc gcctcggcaa ttcgcttgac cagcgccagt cccagcccgc
      361 ttccgccgct gagcttgctg cgggagcggt caatccggta aaacggctcg gttatgcggg
      421 cgatttcttc cggtggaatg cctttgccat ggtcggttac ctcgaacctg ccgccgtatg
      481 cgcttagttc gatttgctga cccggttcgc tggcgttctt ggcattttct accagattgg
      541 ccagcgcgga gcgcatcagg tcaaaatcca ttgccgtacg ctccatcttg cactccgcag
      601 acagcgttac acctttttgt tcgagcgagt cggcggtggt ctcccgcacc gcatcgagca
      661 gcgcggacac gtcttccggg cgcaaggtga tttcgccctg cagcagtacg agcgtcatca
      721 gcttttgcga aagcttttcc agccaccggc tttgatgacg aatccgctca agagagcgtg
      781 tctgcgcctc ctgcggaacc gatctgctca ggagcgtttc cgagcagagc aaaatcgacg
      841 tgaccggcgt tttcagctca tgagaaaggt cgttgataaa cgcctgctgc tgctcggctt
      901 ttctttgcag ttcacgcatg ctcgtctgga ttgcgccggc catactgttg aagttatccg
      961 ccagttcggc gagttcatct tttccggaac agacgatcct gttttcataa tgaccacgcg
     1021 ccagttccga agctgcggct ttcaggctct ggatcggctt gagaaagtac tgcatggcaa
     1081 gcagcatcag catcgccgca aacaacgtga ttgccgttcc cgcgagcgca cacttgacgg
     1141 cgagcatgga aagcgcttca ctctgcgagg tgatgtttcg aaccagcgaa agcgtgtaac
     1201 ccgtttgcgc gcccgtcagt tcgccgacaa caaggaaata tgtctcccct ccgcttttcg
     1261 ccgtcgtgta ctgaacgctc atttcgaaag actcaaacac atactgcttc gccccgccta
     1321 gcgaagccgt tgccggcgcg tcgatgccga ttccgtttgc cagcatatcg tcgccgcgct
     1381 gcagcgtgta ttcttcgttt cctttcattg ctgcgagctg gtacttcaac actgtttgca
     1441 gagtgcgttt cgaagcaccg tttccgacgc tcgctttatc tgcgtactga tcatagaaag
     1501 acgcataatc gctcagcgcg gcggcggtaa tagagtccat cacgttttga cgtgcaaagc
     1561 gaagaataaa caggctgcat acggtaatcg cgcaggcgag cagcgtcacg gagacggcca
     1621 tgaccttcgt tttcagtctc atgctcagtc ctccagccgg taaccaacct tggagaccgg
     1681 caaaatcgcg tcctccaggt gcagtttttt tcgcaggtgc gcaacatgga tatccactgt
     1741 ccgcgttccg ccagcgaagt cgatgcccca gatttcgttg agcagacgtt cccgcggcag
     1801 ggtgcagttt ttgaaccgca ccagcttgac cagcaaatca aattcaagcg gctgcagcgg
     1861 taccgccgtt ccgcattgaa ccaccgtccg tttttccgtg tcgatggtga tatcccgata
     1921 ccgcaaaacc ttattgagct ttccggcgcg gtcgaggatt ttttccattc gaaccagcag
     1981 ttccagaatt tcgaaaggct tgatcatata gtcttccgcg ccgccgcgca gccccctgac
     2041 gcgggacggc aggtcggccc tggccgtca
//
LOCUS       DAOMGY010000303         2066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_61362_length_2066_cov_3.258081, whole genome shotgun sequence.
ACCESSION   DAOMGY010000303 DAOMGY010000000
VERSION     DAOMGY010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61362_length_2066_cov_3.258081"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..854
                     /locus_tag="PLR57_05310"
     CDS             99..854
                     /locus_tag="PLR57_05310"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQQ40909.1"
                     /translation="MNQRKNWLLKAALALVLAAALLPFAACKAEDDITPGPTAQPEQP
                     DVIVNNELAPDEHTIQVTGSGEVIAAPDFSTITISVQGSGPTSEEAALKCEERVQQVT
                     EIAAAQGILPKNMTTAGATLSTHQRESDGAITGYVATDTITIMLTDVSKVNGVLSPII
                     DAGITESYEATYSLTDASAAYSEALAAAMVDAYAKASSIAEAGNVTLGSIASAVETPG
                     EDQLVGIAFESSAIAVSAQVTVVYVITEKPVIP"
     gene            complement(946..1152)
                     /locus_tag="PLR57_05315"
     CDS             complement(946..1152)
                     /locus_tag="PLR57_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40910.1"
                     /translation="MSKYDPLWQQFLNRSEPHVKLTFAEIEQMLGFAIDHSFLNYKKE
                     LLPFGYEVEKISLKEQTVLFRKIS"
     gene            complement(1177..1977)
                     /gene="radC"
                     /locus_tag="PLR57_05320"
     CDS             complement(1177..1977)
                     /gene="radC"
                     /locus_tag="PLR57_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQQ40911.1"
                     /translation="MPDSIHEGHRERIREQYKKSGAEAFLDHQFLELLLTYAIPRRDT
                     NALSHALLDRFGTLEGVVTAEIDQLTMVEGIGEGAAVFLRMQGDLFRRLLLRRTEDAR
                     GNIRLTTPAAAARYAVALLSLSTYENVIAVCLNAKKVVQSCESLQRGTLTEAQIYPRS
                     IAEIALLKRAHGILLIHNHPSGDPTPSQDDADATELVKAALGSVGVQLYDHLIVGGAN
                     AYSFTTRAVIGVSGAETEVHSLEDFQSHYAAQSSKPLPLVRVMEPYED"
BASE COUNT          443 a          575 c          605 g          443 t
ORIGIN      
        1 ctggagatgc gcaaaaagct cgcatgtctt gcggcgaata acctgaagac atttttacag
       61 ggcggaactc tcaaccgggt agatttggag gcaaaagcat gaaccaacgc aaaaactggc
      121 tgttgaaagc cgcgcttgct ctcgttctag cggcggcgct gctaccgttt gccgcatgca
      181 aggcggagga cgacattacg ccggggccga ccgcgcagcc ggaacagccg gacgttatcg
      241 tcaacaacga acttgcaccg gacgagcaca ccattcaggt tacgggcagc ggcgaagtga
      301 ttgccgcgcc ggatttttca actattacga tctcagttca gggttccggc ccgacttccg
      361 aggaagctgc gctcaagtgc gaggaacgcg ttcagcaggt taccgaaatc gccgccgcgc
      421 aggggattct gccgaagaac atgaccacgg cgggcgcaac gctgagcacg catcagcgcg
      481 aaagcgacgg ggcgattacc gggtatgtgg cgaccgacac gattaccatc atgctcaccg
      541 atgtcagcaa ggtcaacggc gtgctctcgc cgattatcga cgcgggcatt acggaatcct
      601 acgaagcgac ctacagttta acggacgctt ccgccgcgta ttcggaagcg cttgccgccg
      661 caatggtgga tgcgtacgca aaagcttcct ccattgccga agcgggaaac gtaacgctcg
      721 gctcgattgc ttccgccgtg gaaacgccgg gggaggacca gctcgtcggc attgcgtttg
      781 aaagcagcgc catcgcggtt tccgcgcagg tgacggtggt ttacgtcatc accgaaaagc
      841 cggtcatacc gtaagccgag aaagatttgg tgaaacaaaa gcggcggggg aacccgccgc
      901 ttgttttatg gagtgtcgtt ctttgaatct agtttttttt acaaattacg agattttccg
      961 aaacaatacc gtctgctctt tcagcgaaat cttctccact tcgtagccaa aggggaggag
     1021 ttctttttta tagttcagaa acgaatggtc gatggcaaag ccgagcattt gctcgatttc
     1081 ggcaaacgtt agtttgacat gtggttccga ccgattaagg aactgctgcc ataacggatc
     1141 gtatttactc ataatttcct ccgtttttga gtgaaactag tcttcgtacg gctccatgac
     1201 gcgtacgagc ggaagcggct ttgacgactg cgcggcatag tgcgactgga agtcctccag
     1261 agagtgcact tccgtttccg cgccggaaac gccgatgacc gcgcgcgtgg taaaactgta
     1321 cgcgttggcg ccgccgacga tcaggtggtc gtagagctgc acgccgacgc tccccagcgc
     1381 cgccttgacc agctccgttg cgtccgcgtc atcctgcgag ggggtcgggt cgccggaagg
     1441 atgattatgg atcagcagta tgccgtgcgc gcgcttgagc agggcgattt ccgcgatgct
     1501 cctggggtag atctgtgctt ccgtcagcgt gccgcgctgg agcgattcgc aggactggac
     1561 gacttttttt gcgttcaggc agaccgcgat gacgttttca tacgtgctca gcgagagcag
     1621 cgcaaccgcg taccgcgcgg cggcagcggg cgtggtcagg cggatgttac cgcgcgcgtc
     1681 ctcggttctg cgaagtaaca gcctgcggaa caaatctcct tgcatgcgca gaaaaaccgc
     1741 cgcgccttcg ccgatgcctt ccaccatggt cagctggtcg atctccgcgg tcacaacgcc
     1801 ttccagcgtg ccgaagcgat ccagcagtgc gtgagagagc gcgttggtgt ccctgcgcgg
     1861 gatggcgtac gtcagcagca gttcgaggaa ctgatggtcc aggaacgctt ccgcgccgct
     1921 ctttttatac tgttcgcgaa ttcgctcgcg atgcccttca tggatggaat ccggcatgtt
     1981 gtctgctccc cacattccat atcagtatag tagattcggc aaatcatgtc aaaagatacc
     2041 caagcgtgaa ttgtttgcaa cactcc
//
LOCUS       DAOMGY010000304         2057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_61824_length_2057_cov_3.475524, whole genome shotgun sequence.
ACCESSION   DAOMGY010000304 DAOMGY010000000
VERSION     DAOMGY010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61824_length_2057_cov_3.475524"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /gene="ligA"
                     /locus_tag="PLR57_05325"
     CDS             <1..1217
                     /gene="ligA"
                     /locus_tag="PLR57_05325"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQQ40912.1"
                     /translation="CSTAADLMREIRLAEQTRGKLDFLIDGMVVKVTDFALREAMGET
                     EKFPRWAMAFKFAAEETTSIVEDVTWEVGRTGKLTPLAHLTPVELAGATIQRATLNNY
                     DDIQRKRVKCGSRVFIRRSNDVIPEILGAVPDDTATEEILKPTRCPECGAHVEERGAH
                     IFCTNSLSCKAQITLRIAHYASRDAMDIESLSDKTAAQFIEELNISSIPELYALTHEQ
                     LVSLSRFGAKKAQNLLDALEKSKTRPLGAFLFALGIPNVGFKTAKDLAKRFGTLEAVR
                     KATREELLAIPDVGEIVADSILKFFSDASIADQIDTLLSYGVSPQSEQAANTSSPISG
                     KTIVVTGTLPTLGRREAEALIEQNGGKSAGSVSKKTDYVLYGESAGSKLDKAKELNIP
                     LLTEEEFLKLIG"
     gene            1314..>2057
                     /locus_tag="PLR57_05330"
     CDS             1314..>2057
                     /locus_tag="PLR57_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HQQ40913.1"
                     /translation="MLRLGKLDNDDLERLVLHKFRRVRPESLSAPAIGQDCATLDIQN
                     DLIVLSSDPITSASSAHLGRLTVHVCCNDAAAAGAEPVGLLVTLLLPPTSSLEEIGRI
                     ADDLSTAAQLANVDILGGHTEVTDSVTRPVTSATVVARQARGKALVGMKPGDEIVMTK
                     SAAIEGTTILAEDFPHLLGSIPSETIAKARAFSGKLSIVPESAIAMQFGAVAMHDVTE
                     GGVLGAVWELAYASGCGAEIFSDRISIHAE"
BASE COUNT          487 a          607 c          578 g          385 t
ORIGIN      
        1 tttgttcaac cgccgccgac ctcatgcggg agattcgcct tgccgagcag acgcgcggca
       61 agctcgattt tttgatcgac ggcatggtcg tgaaagtgac cgacttcgcg ctccgcgaag
      121 cgatgggcga aacggaaaag ttcccgcgct gggcgatggc gtttaagttc gccgccgaag
      181 aaacgacctc catcgtggaa gacgttacct gggaagtcgg gcgcacgggc aagctcacgc
      241 cgcttgcgca cctcacaccc gtcgaacttg cgggcgcgac cattcagcgc gccacgctga
      301 acaactatga cgatatccag cgcaagcgcg tcaaatgcgg ctcacgcgta ttcatccgca
      361 ggagcaacga cgttatcccc gaaattctcg gcgcggtgcc ggatgatacc gccaccgagg
      421 agattcttaa acccacgcgc tgcccggaat gtggcgcgca tgtggaagag cgaggcgcgc
      481 acatcttctg cacgaactcg ctctcctgca aggcgcagat cacgctccgc atcgcccact
      541 acgcttcgcg ggacgcgatg gatattgaaa gtctcagcga caagaccgcg gcacagttca
      601 ttgaagagct caatatttcg tccattcccg agctctacgc gctgacgcat gaacagctcg
      661 tttcgctgag ccgcttcggc gcaaagaaag cgcagaacct gttggacgcg ctcgaaaaga
      721 gcaagacccg cccgctcggc gcgttcctct ttgcgctcgg catcccgaat gtcggcttca
      781 agaccgcgaa agatttagcc aaacgcttcg gcacgctgga agcggtgcga aaagcaacgc
      841 gcgaagaact gctcgccatc ccggacgttg gcgaaatcgt agcggacagc atcctcaagt
      901 tctttagtga cgcttccatc gcagatcaga tcgacacgct gctttcctac ggggtttctc
      961 cgcagagcga acaggcggca aatacctctt cgcccatctc cggcaagact atcgtggtca
     1021 ccggtacgct gccaacgctg gggcggcgcg aagccgaagc gctcattgag cagaacggcg
     1081 gcaagtcggc gggtagcgta agtaaaaaga ccgattacgt gctttacggc gaaagcgcgg
     1141 gcagcaagct cgacaaagcc aaagagctga atatcccctt attgacagaa gaagaatttt
     1201 taaaattgat cggctgagca cagctaaact gacgaaaaaa agaagaattt ttaaaattaa
     1261 acggctgagt acagccaaac ggacgataaa taagattgga gaacacatac tgcatgctcc
     1321 ggctcggtaa acttgacaac gacgatttag aacgactggt actccataaa ttccgccgcg
     1381 taaggccgga atctctttcc gcgcccgcca tcgggcagga ctgcgcgacg ctcgacattc
     1441 aaaacgattt aatcgtatta agcagcgacc ccatcacctc cgccagttcg gcgcatttgg
     1501 gcaggctcac agtgcatgtt tgctgcaacg acgcggcggc agccggcgcg gaaccggtcg
     1561 gcctgctggt taccctgttg cttccgccga cgagcagtct cgaagagatc ggtaggattg
     1621 cggacgatct ttccactgca gcgcagctcg ccaacgtgga tattctcggc gggcacacgg
     1681 aagtaacgga ttccgttacc cgtcccgtca cgagcgcgac ggtcgttgcg cggcaggcgc
     1741 gcggcaaagc gctcgttggc atgaagccag gcgatgaaat cgttatgaca aaatccgccg
     1801 ccatcgaagg aacgaccatc cttgcggaag attttcctca cttgcttggt agtataccat
     1861 ccgaaacgat cgcaaaagca cgcgcctttt ccggcaagct cagcatagtg ccggaaagcg
     1921 cgatcgccat gcagtttgga gctgtcgcca tgcacgacgt aaccgaaggc ggcgttctcg
     1981 gcgcggtttg ggagctggct tacgccagcg gatgcggcgc ggaaatcttt tccgaccgca
     2041 tttccatcca cgcggaa
//
LOCUS       DAOMGY010000305         2052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_62060_length_2052_cov_2.909364, whole genome shotgun sequence.
ACCESSION   DAOMGY010000305 DAOMGY010000000
VERSION     DAOMGY010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62060_length_2052_cov_2.909364"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PLR57_05335"
     CDS             <1..641
                     /locus_tag="PLR57_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HQQ40914.1"
                     /translation="WNDGYRKIIFVNNHGHLWMLESAIQEFCKRYQLPGIFRVIEWHR
                     AVREFFYPIDSELSLGTHFIHADEAETSVALLLFKDMVNMAAVQDSWGEPLLPDEHFD
                     KSVDPFRRPNRWSEGEGHSAIERAATPEGVVGCPSTSSARKAKRPIVAILKYLTLLNE
                     QILDAYPSGKVPPVEKITLRKEEEMAPFLKEPLSEGWKSVDELPRIGIFEHL"
     gene            686..1750
                     /locus_tag="PLR57_05340"
     CDS             686..1750
                     /locus_tag="PLR57_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQQ40915.1"
                     /translation="MKAKVAFLHKPFDLRVEEVEVPALKDNQVLVQVGACGICGSDVE
                     CFEGKSKEGRYDIAPYTPGHEWGGKIAAVGAGVQTLKVGNKVTGDCVMACGVCRNCKD
                     GLMPSACLNMREAGFRPDSPGGMGEYMVIEEQYVHRVPDNWTYADAAWVETFSIGYFG
                     IWGNGGYIDASDIAIIMGCGPVGVSAVMTAKMSGAKVIVVEPFEKRRAIARKFGADYV
                     VDPTAVDLVPEIERLTDGRMGTVVVEASGNDKAIASLFEVAGHSARVRLIGHSIGRKV
                     PVEIGRTIWRTLSISGSGGTRNFGQRTIRFMSALKEKGIDFGELNTHYYPFGQIHEAF
                     DVACKLKSDAFKVMLTFDVK"
BASE COUNT          427 a          589 c          631 g          405 t
ORIGIN      
        1 tttggaacga cggctaccgc aagatcatct tcgtcaacaa ccacggccac ctttggatgc
       61 tggaaagcgc gattcaggaa ttctgcaagc gctatcagct gccgggtatc ttccgcgtga
      121 ttgaatggca ccgcgcagta cgcgagttct tctacccgat cgacagcgaa ctcagcctcg
      181 gcacgcactt tatccacgcg gacgaagcgg agacgtccgt tgcgcttttg ctctttaagg
      241 atatggtcaa catggccgcc gtgcaggatt cctggggcga gccgctcctg ccggacgagc
      301 acttcgataa atccgtcgat cccttccgcc gcccgaaccg ctggagcgag ggcgaggggc
      361 acagcgccat cgagcgcgcg gcaacgccgg aaggcgtggt cggctgcccg tctacttcca
      421 gcgcgcgcaa ggccaagcgc ccgatcgtcg cgattctgaa gtacctaacg ctcctcaatg
      481 agcaaattct tgacgcgtac ccttccggca aagttccgcc ggtggagaag attacgctcc
      541 gcaaggaaga ggagatggcg ccgttcttaa aagaaccgct cagcgaaggc tggaagtccg
      601 tagacgaact gccgcgaatc ggcatcttcg agcacctgta agaaaaactc tgattttata
      661 caaatattga aaggatgaga atcgtatgaa ggcaaaggtc gcgtttctgc ataagccctt
      721 cgacctccgg gtcgaggagg ttgaggttcc cgcgctcaag gacaaccagg tgctcgtgca
      781 ggttggcgcc tgcggcatct gcggctcgga tgtggagtgc tttgaaggca agtccaagga
      841 agggcgttac gacatcgccc cctatacgcc gggtcacgaa tggggcggca aaatcgccgc
      901 cgtcggcgcg ggcgtgcaaa cccttaaggt gggcaacaag gtcacgggcg actgcgtcat
      961 ggcctgcggc gtctgccgca actgcaagga cgggctcatg ccgtccgcct gcctgaacat
     1021 gcgcgaagcg ggcttccggc ccgattctcc cgggggcatg ggcgagtaca tggtcatcga
     1081 ggagcagtat gtccaccgcg tgccggacaa ctggacgtat gcggacgcgg cgtgggttga
     1141 gacgttctcc atcggctact ttggtatctg gggcaatggc ggctatatcg acgcttccga
     1201 tatcgcaatc atcatgggtt gcggcccggt cggcgtttcc gccgtgatga ccgcaaagat
     1261 gagcggagcc aaggtgatcg tggtcgaacc gtttgaaaaa cggcgcgcca tcgccaggaa
     1321 gttcggcgcg gactatgttg tcgatcccac ggcggtcgat ctcgtgccgg aaatcgagcg
     1381 gctcaccgac gggcgcatgg gcaccgtggt ggtggaagcc agcggcaacg acaaggccat
     1441 tgcgtcgctc tttgaggtgg cggggcacag cgcgcgcgtg cggctgatcg gccactccat
     1501 cgggcgtaaa gtaccggtag aaatcgggcg cacgatctgg cggacgctct ccatcagcgg
     1561 ttccggcggc acgcgcaact ttggccagcg caccattcgc tttatgagcg cgctgaagga
     1621 aaagggcatc gactttggcg aactcaacac gcattactac ccgttcgggc agattcacga
     1681 agcgttcgac gtggcctgca agctcaagtc cgacgcgttc aaggtcatgc tgacgttcga
     1741 cgtaaagtaa aatctctcag cttccgccgc gctttttgga ttttgcgcgg gggatggaat
     1801 aacctcctaa ttctcgtttc cccttttccc tctgaacatg cgcgcgggcg gctgattgcg
     1861 cactgtaacc gtccgcgcgc aaaccttttt tcagcaattt catgctttgg caaggccgga
     1921 accgggtatt tagatgaacc gaacggagga cgagaccatg tttcaaccca aactctgcat
     1981 gacggtggtc agacagcgcg gagcgggcgc gccgtttctt tacggcggga atctcactaa
     2041 aggcattgaa gc
//
LOCUS       DAOMGY010000306         2045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_62389_length_2045_cov_2.637186, whole genome shotgun sequence.
ACCESSION   DAOMGY010000306 DAOMGY010000000
VERSION     DAOMGY010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62389_length_2045_cov_2.637186"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /gene="mutS"
                     /locus_tag="PLR57_05345"
     CDS             <1..1536
                     /gene="mutS"
                     /locus_tag="PLR57_05345"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQQ40916.1"
                     /translation="NTHIITIEDPIEYLHRHNRSVVNQREINADTNSFSAALKRVFDQ
                     LDEMEDIFSLLARAICDEPPASLKDGGFIRAGYNAEVDDLRSIANGSESWLERFEAQE
                     REQTKIKSLRVGYNRVFGYYIEVTKSYLQLVPYHYERKQTLANAERFVTPELKEMEQK
                     IIGARDRLVALENELFLEIRQVLLASTERMQLNGALLAELDAYVSLAEVASNNGYCRP
                     KLNQQGRVQIVNGRHPVIERALKEPFVPNDVLLDRTENRLLIITGPNMAGKSTYMRQI
                     ALITLMAHVGSFVPAQSADIAIVDRIFTRIGASDDLSSGQSTFMVEMSEVANILHNAT
                     RDSLLILDEIGRGTSTFDGLSIAWSVLEYISDAKRCGAKALFTTHYHELTELEGDIAG
                     VKNFRVTVREIGEDIVFLRKIVRGGADKSFGIQVARLAGLPEEVLSRAKEILSELESA
                     DIAAKKAVPATKVLASVQPSLLDESKEESALRIMREMDVDHLTPLEALNQLYALHAML
                     KSK"
     gene            1563..>2045
                     /gene="mutL"
                     /locus_tag="PLR57_05350"
     CDS             1563..>2045
                     /gene="mutL"
                     /locus_tag="PLR57_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015734656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQQ40917.1"
                     /translation="MPKIHVLPPHIANQIAAGEVVDRPSSVVKELVENSIDAGATAVT
                     IEIENGGVDLIRILDNGLGIADEDCETAFLRHATSKIASAGDLFSIRSLGFRGEALAS
                     IAAVSRVRLQTQTQEDELGTLIRYEGGALVEHRRTGGTRGTSVEVRNLFYNVPARLKF
                     M"
BASE COUNT          477 a          545 c          602 g          421 t
ORIGIN      
        1 aatacccata tcatcactat tgaggacccc atcgaatact tgcaccgcca caaccgcagc
       61 gtggtcaacc agcgcgaaat caacgcggac accaattcgt tcagcgcggc gctcaagcgc
      121 gtgttcgatc agcttgacga aatggaggat attttctcgc tccttgcccg cgcaatctgc
      181 gacgaacctc ccgcgagcct caaggacggc ggatttatcc gtgcgggcta caacgcggaa
      241 gtagacgatc tcagaagcat tgcgaacggt tcggaaagct ggctcgaacg ctttgaagcg
      301 caggagaggg agcagaccaa gattaaatct ctccgcgtgg gctacaaccg tgtattcggg
      361 tactatatcg aagtcacgaa gtcctatctc cagctcgttc cgtatcacta tgagcgcaag
      421 cagacgcttg ccaacgccga gcgttttgtt acgccggaac ttaaggagat ggaacagaag
      481 attatcggcg caagggatcg tttggttgcg ctggagaacg agctgttttt agagattcgt
      541 caggtgctgc ttgcgagtac ggaacggatg cagctcaacg gcgcgctatt agccgaactg
      601 gacgcttatg tttcccttgc ggaagttgcg tccaacaacg gctactgccg tccgaagctc
      661 aaccagcagg ggagagtgca gattgtcaac ggcaggcatc cggtgattga acgagcgctc
      721 aaggaaccgt tcgtgccgaa cgacgtgctg ctcgaccgaa ccgaaaaccg gcttttgatc
      781 atcacaggcc cgaacatggc cggcaagagt acctatatgc gacagatcgc gctgattacg
      841 ctcatggcgc acgtggggtc gtttgttccg gcgcagtcgg cggatatcgc gattgtcgat
      901 cgcatcttta cccgcatcgg cgcgagcgac gacctctcat ccggccagag cacattcatg
      961 gtggagatga gcgaggttgc gaacatcctg cacaacgcta cgcgggacag cctgcttata
     1021 ctcgacgaaa ttggccgcgg aaccagcacg ttcgacggct tgagcattgc ctggtcggtg
     1081 ctggaataca tctccgacgc gaaacgctgc ggcgcaaaag cgctgtttac tactcactac
     1141 cacgagctca ccgagttgga aggcgacatc gcgggggtta agaacttccg tgttaccgtt
     1201 cgggaaatcg gcgaagatat cgtgtttctg cgcaagatcg tgcgcggggg agcggataag
     1261 agctttggta ttcaggtggc gcggctcgcc ggacttccgg aggaagtgct atcgcgcgcc
     1321 aaagaaattc tttccgagct ggagtccgcg gatatcgcgg cgaaaaaagc cgttccggct
     1381 acaaaagtac tggcaagtgt acaaccttct ttgctggatg aaagtaaaga ggagagcgcg
     1441 ctgagaatta tgcgcgaaat ggatgtcgat cacctgactc ctctggaggc gctcaatcag
     1501 ctgtacgctc tccacgcgat gctaaaatcg aaatagtaca gaccgttata ttggagcaac
     1561 gaatgcctaa gattcatgta ctgccgccgc atattgcaaa ccagattgcc gcgggcgagg
     1621 tcgtagaccg gccgtcgtcc gtggtgaagg agcttgtgga aaactccata gacgcgggcg
     1681 cgacggcggt tacgattgaa atcgaaaacg gcggggtcga tctcatccgc attctcgata
     1741 atggactcgg cattgcggac gaggattgcg agaccgcgtt tttacggcat gcaaccagca
     1801 aaatcgcttc ggcgggggat ttattttcca ttcgctcgct cggttttcgc ggtgaggcgc
     1861 tcgcttccat cgcggcggtt tcccgcgtac ggctgcaaac gcagacgcag gaagacgagc
     1921 ttggaacgct tatccgttac gaaggcggcg cgctggtcga acaccgccga accggcggaa
     1981 cgcgcggcac gagcgtcgaa gtaaggaatt tgttctacaa tgtgcccgcg cggctcaagt
     2041 ttatg
//
LOCUS       DAOMGY010000307         2043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_62483_length_2043_cov_2.898893, whole genome shotgun sequence.
ACCESSION   DAOMGY010000307 DAOMGY010000000
VERSION     DAOMGY010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62483_length_2043_cov_2.898893"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PLR57_05355"
     CDS             complement(<1..947)
                     /locus_tag="PLR57_05355"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQQ40918.1"
                     /translation="MIAAIAGCSSAEEAAPVATATPEVAIITAAPVITNSDPYTLPIP
                     TSETVPAGYTAVPLENTSVSLFAFTDAQGEIQYRVFGGYSELTNGVETQTFTGFYPSD
                     ETGALLNASDGFIDTATEDFGVCTPSAMPETLRLKTGYTALEIVGAYADENGAKYVYG
                     SFGEGEGAFYPAGADGTMIPGALKSTVSIAVPAYMPETPPETDGERMLIVYIGTQSVV
                     CFKAENGEWTEERVMICSTGRSKLLTPRGTFALMRQYLYKKMGQVDGVNVYSQYASRI
                     TGSYLFHSVPIGGENRYSQENGKHQMIVEYYERLGTPASG"
     gene            934..1197
                     /locus_tag="PLR57_05360"
     CDS             934..1197
                     /locus_tag="PLR57_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40919.1"
                     /translation="MAAIIVIASSKNAAFFQFDFIFMGIIIAKDSQEYNICPVNCGFS
                     SQYIKTISSRSLKAVSAARKAIYAKANTAFTDIARHAKIWVNE"
     gene            1234..>2043
                     /locus_tag="PLR57_05365"
     CDS             1234..>2043
                     /locus_tag="PLR57_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQQ40920.1"
                     /translation="MLVVALLPLTLLRPGRARAEETDPLENLEVPNCIVVDQNDPTIV
                     LYERNADDKSIPGSTMKIMTCILSIELCTDLEQKVTITGQAASMKDTNSLMGVIRGEE
                     LTVRELLYGLMLESGNDAALMLALTFGGSVEGFAELMNQKAAELGMTNTYFINASGAY
                     KSGQYSTARDMAILTCYAMKNELFRTLASTVHYTIEPNNVRKRSMEMTNSNKLISDPE
                     DSSLFYEYATGVKTGSTELGGKCLVASATKDGASVIAVMLGVAEGGSKLARM"
BASE COUNT          496 a          536 c          554 g          457 t
ORIGIN      
        1 ccggaagcgg gcgtaccaag gcgctcgtag tattcaacga tcatctgatg cttgccgttt
       61 tcctgagagt aacggttttc cccgccaatc ggcacggaat ggaacaggta actgcctgta
      121 atgcggctgg cgtattgaga atacacgttc acgccgtcta cctgccccat ctttttatac
      181 agatattgtc tcatcaatgc aaacgtgccg cgcggggtca gcagtttact gcgtcccgtg
      241 gagcagatca taacgcgttc ttccgtccat tccccgtttt ccgccttaaa gcaaaccacg
      301 gactgtgttc cgatatagac gatcagcatg cgctcgccgt cggtttcggg cggcgtttcc
      361 ggcatgtagg cgggaacagc gatgctaacg gtggacttga gcgcaccggg aatcatggtt
      421 ccgtccgcac cggcaggata gaacgcgcct tctccttccc cgaacgagcc gtagacatac
      481 tttgcgccgt tttcgtcggc ataggcgcct acaatctcca gagccgtata accggttttc
      541 agccgaaggg tttccggcat ggcggaaggc gtacaaacgc caaaatcttc tgtggcggta
      601 tcgatgaacc catcggatgc gttcaaaaga gcacctgttt cgtccgaagg atagaaaccg
      661 gtgaacgtct gcgtttcaac tccgttggtc aattcgctgt aaccgccgaa tacgcggtac
      721 tgaatttcac cctgtgcgtc cgtgaacgca aagagagaaa cggaggtgtt ttcaagcgga
      781 accgccgtat agcccgcggg aaccgtttcc gatgtgggga ttggcagcgt gtaaggatcg
      841 ctgttggtga tgacgggagc ggccgtaatg atggcgactt ccggagttgc ggtagcaacg
      901 ggcgcggctt cctccgcgga ggaacaaccc gcaatggcag cgatcatagt gatcgcgagc
      961 agcaaaaacg ccgctttttt ccagttcgac ttcatattca tgggcataat tatagcgaaa
     1021 gattctcaag aatacaatat ttgtccggtg aactgcggtt tttcttcaca atatatcaaa
     1081 actatatcga gtcgttcttt aaaagcagtg tctgctgcaa ggaaagcaat ctacgcaaag
     1141 gcgaataccg cgtttacaga tattgcgcgt catgctaaaa tatgggtgaa tgaatagaac
     1201 ggaatacatg aaatgagaag agttttctgt gctttgctcg tcgtcgcgct tctgccgctt
     1261 accctgctgc ggccgggccg tgcacgcgcg gaagaaacag acccgctgga aaatctggag
     1321 gtgcccaact gcattgtcgt ggatcaaaac gatcccacaa tcgttctcta cgagcgaaat
     1381 gcggacgata aaagcatccc cggcagcacg atgaaaatca tgacctgcat tctaagtatt
     1441 gagctttgca ccgatctgga gcaaaaggta acgattaccg gccaggccgc gagcatgaag
     1501 gatacaaact cgctcatggg cgttatccgc ggcgaagagc tcaccgtgcg cgagcttctt
     1561 tacggactga tgctggaatc gggcaacgat gcggcgctca tgctggcgct gaccttcggc
     1621 ggcagcgtgg aaggctttgc ggagcttatg aaccagaaag cagcagagct tggcatgacg
     1681 aacacgtatt tcatcaacgc cagcggagcg tataagagcg gccagtattc caccgcgcgc
     1741 gatatggcaa ttttgacctg ttatgccatg aaaaacgaac tgtttcgcac gctggcttcc
     1801 accgttcatt acaccattga accaaacaac gtccgtaagc gctccatgga gatgacaaac
     1861 tcaaacaagc tcatctccga tcccgaggac agttcgctgt tttacgagta tgcaaccggt
     1921 gtaaaaaccg gatcgacgga acttggcggc aaatgccttg ttgcttccgc taccaaagac
     1981 ggcgcgagcg tcatcgcggt catgcttggc gttgccgaag gcggtagcaa actcgcgcgc
     2041 atg
//
LOCUS       DAOMGY010000308         2041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_62566_length_2041_cov_3.826788, whole genome shotgun sequence.
ACCESSION   DAOMGY010000308 DAOMGY010000000
VERSION     DAOMGY010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62566_length_2041_cov_3.826788"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PLR57_05370"
     CDS             complement(<1..548)
                     /locus_tag="PLR57_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6144 family protein"
                     /protein_id="HQQ40921.1"
                     /translation="MFDIRAIQEQVLHENVRAASNEQTASEVVYCAPTDTAMSEAEWV
                     ASSMQKLEHAFDKPVVEQIRMRCQCGYGMEKRLELLKELIAASSSLEEFAGHEKARAA
                     GMFYRDDALYLQFFFCPCPMLATVDRLESDTWCQCTRGYTKVLFERAFGCEVEVELLK
                     SIKTGGDVCLQRITPKGAVWRG"
     gene            complement(864..1466)
                     /locus_tag="PLR57_05375"
     CDS             complement(864..1466)
                     /locus_tag="PLR57_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HQQ40922.1"
                     /translation="MRRRKIISQKGGNHVPTQTLTSAQRDELLQTLQSRFEKHPARHP
                     SLAWPQILRRLEGSPEKLFSLYEMERTGGEPDVVGIDEKTQAVLFFDCAQETPAGRRN
                     VCYDRAALDSRKNFKPETSAMELADAMGIALLTEDQYRFLQQLGEFDLKTSSWILTPP
                     EIRKRGGALFCDRRYDHVFTYHNGAESYYAVRGFRGCVLV"
     gene            complement(1444..>2041)
                     /locus_tag="PLR57_05380"
     CDS             complement(1444..>2041)
                     /locus_tag="PLR57_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQQ40923.1"
                     /translation="ARVSDGVWDEPFELHLSDADGAAIEHLLSLLDEEQNRLERDFSD
                     FEGRETVYAEYIRLIFIHLMRLREQGCGTSAFLRAAKAYIDAHLAEGLRAETISSALH
                     MGKTHLMRRFHAESGISLHQYILKLRLQRARRLLSRGVGAVESSQLSGFSDYTTFYKA
                     FQREYGLSPSSFYGGFRMTQTEVDGKLHKDYNEEKEDN"
BASE COUNT          442 a          568 c          554 g          477 t
ORIGIN      
        1 tatccgcgcc agaccgcgcc tttcggtgta attcgctgga ggcagacgtc gccgcccgtt
       61 ttgatgcttt tgagcagctc cacttcgacc tcgcagccga acgcccgctc aaagagcacc
      121 tttgtgtagc ctctggtgca ttgacaccat gtgtcggatt ccaatcggtc tacagtggcg
      181 agcatggggc aggggcagaa gaagaactgc agatacagcg cgtcgtcgcg ataaaacatt
      241 cccgccgcgc gcgctttttc gtggcctgca aattcttcga gactggacga cgcggcgatc
      301 agttctttta gcagttcaag ccgcttctcc ataccatacc cgcactgaca gcgcatacgg
      361 atttgctcga caacgggttt gtcgaacgca tgctccagtt tttgcatgga ggacgcgacc
      421 cattccgctt cactcattgc cgtatctgtg ggagcgcagt aaacgacttc ggacgctgtc
      481 tgttcattgc tcgcggcgcg gacgttttcg tggagtacct gttcttggat tgctcgaatg
      541 tcaaacatat aataaacctc attctttctt catcagttgt atgcaggcct gctcaaacag
      601 gcgcatgttt acgcgtcaaa aaagaaccgc gtttttgcgc tgctgttcga atgcacttga
      661 tttttgtgaa aatcaaatca ggctatttat catagccgca gtcgggaaac ggataagaaa
      721 ttatgaacgg aatggttcga gcaagaccaa agttcctttc agaaaaaatg ctacggtagt
      781 aataccacaa cgccagctgt ctgtcaagcg aatatacgac ggtcattttg cgtaaagcgg
      841 gaaatgccgc aacactgaac tgtttatacc aatacacagc cgcggaatcc tcgcacggcg
      901 taatacgatt ccgcgccgtt gtgataggtg aatacatggt cgtacctgcg gtcgcagaaa
      961 agcgcgccgc cgcgtttgcg aatttccggc ggggtcaata tccagctgga agttttcagg
     1021 tcgaactcgc ccaactgctg gagaaaacgg tattgatctt ccgtcagaag cgcaatcccc
     1081 atggcgtccg ccagttccat tgcgctggtt tcgggtttga agttcttgcg cgagtcgagc
     1141 gccgcgcggt cgtagcatac gtttctgcgc ccggctggcg tttcttgcgc gcagtcgaaa
     1201 aagagcacgg cctgcgtttt ttcgtcgatg ccgacaacat ccggctctcc gccggtgcgc
     1261 tccatttcat aaagcgaaaa gagcttttcg gggctgccct ccagccgccg caaaatctgc
     1321 ggccatgcga gggatgggtg acgggcgggg tgcttttcaa agcgcgattg aagcgtctgc
     1381 agcagttcat cgcgctgcgc ggaagtgagc gtttgagtag gcacgtgatt tcctcctttt
     1441 tggctaatta tcttccttct cctcattgta atccttatgc aatttgccgt ccacctcggt
     1501 ttgcgtcatg cggaatcctc cgtagaagct cgaaggcgaa agtccgtact cgcgctgaaa
     1561 cgccttgtaa aacgttgtat aatccgaaaa gccggacaac tgcgaactct ccaccgcgcc
     1621 gacgccgcgc gaaagcagcc tccgtgcccg ctgaaggcgc agcttgagaa tgtactggtg
     1681 gagagaaatg ccgctttccg cgtggaaacg gcgcatcagg tgcgttttac ccatgtgcag
     1741 cgcggaggag atggtttccg cgcgaagacc ctccgccaga tgcgcgtcga tatatgcctt
     1801 tgcggcgcgc agaaacgcgc tggttccgca gccctgctcc cgcaggcgca tcaggtgaat
     1861 gaaaatcagc cggatatact cggcgtatac cgtttcgcgt ccctcgaaat cggaaaaatc
     1921 ccgttccagc cggttttgct cctcgtccag cagagagagc aggtgttcaa tcgccgcgcc
     1981 gtcggcgtcc gaaaggtgca gttcaaacgg ttcgtcccaa acgccgtccg aaacgcgggc
     2041 g
//
LOCUS       DAOMGY010000309         2041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_62582_length_2041_cov_3.091641, whole genome shotgun sequence.
ACCESSION   DAOMGY010000309 DAOMGY010000000
VERSION     DAOMGY010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62582_length_2041_cov_3.091641"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLR57_05385"
     CDS             complement(<1..250)
                     /locus_tag="PLR57_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQQ40924.1"
                     /translation="MTITLNDFIARITTSVPLLITVLLTLGVIMVNGWTDAPNAIATC
                     ISTRSMPPRAAIFMAAGFNFLGVFVMTLLNATVAQTIYN"
     gene            522..1535
                     /locus_tag="PLR57_05390"
     CDS             522..1535
                     /locus_tag="PLR57_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQQ40925.1"
                     /translation="MDDGSPIGFIVLIILLILISGYFSSAETAMATVNRIRIMSLSEN
                     GNKRAKRVLYILDHFDQALTTILIGNNITHIACASAATLFVSKLWGVSAVTAATFVTT
                     LVVFLFAEMLPKRIAKAISEPYALLVSGSLIFFMKILSPVARVFSALGRFVGKPFRKQ
                     EEPPTVSEDELYDIIETVATEGVIDEEKTELVQSALEFSDTSAFDILTPWDRVVKVTT
                     DMTQAQVLDSIKNNVHSRLPVVNDKNKPVGMLQIRKYLKAYLKGAVRLEDVMDDCRFV
                     SASTAIDDLLPLMSAQRTQIAIVTGDEEEILGIVSVEDILEELVGEIYDEDDTFDAGG
                     TAS"
     gene            1532..>2041
                     /locus_tag="PLR57_05395"
     CDS             1532..>2041
                     /locus_tag="PLR57_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HQQ40926.1"
                     /translation="MTNVSFWQYLGIVGLLGLLAFFSGSEIAFAKANRMRLRAATEEG
                     NVLSKIAYYIYQSYDNALVTILIGNNLVNIAASSVATVIAIALMGDSGAWVATLAMTV
                     LIIIFGEIVPKIVASQVPEGFAKAVSIPLRALMTLLFPLVWVLSLLLKGVDRLLKKRV
                     GETPSVTEED"
BASE COUNT          473 a          553 c          517 g          498 t
ORIGIN      
        1 tgttgtaaat cgtctgcgca acggttgcgt taagcagcgt catgacgaac acgccgagga
       61 agttgaatcc cgccgccata aagattgccg cgcgcggcgg cattgagcgg gttgagatgc
      121 aggtcgcaat cgcgtttggc gcgtccgtcc agccgttgac catgataacg ccgagcgtca
      181 gcaaaaccgt tatcaaaagc ggtacgctgg tcgtaatccg cgctatgaag tcgttaagcg
      241 ttattgtcat tgatatccta tcccccgaat attaccaaat tcttacccgt ttatcattat
      301 aacacgaaga aaacccaaac cttacacgat cttgatattt ttggtattta tcaagaatat
      361 tttacattga tgggtttagc tgtgtaaaat cacccgtttt tgcatatcat gctgcacaat
      421 caattgaaaa atgcacataa tcgcccgctt tccttgcttt ttgtcccccg cgcatgtata
      481 atcaaaaaag tatttcattg actaggagtg tgatttcgtc catggacgac ggcagtccga
      541 taggattcat cgttctgatt atccttctca ttctcatcag cgggtatttt tcgtcggcgg
      601 aaaccgcaat ggcaaccgtc aaccgcatac gcatcatgag cttgagtgaa aacggcaata
      661 aacgcgccaa gcgggtttta tacatccttg atcacttcga tcaagcgctg accaccattc
      721 tcattggcaa caacatcacc catattgcct gcgcatccgc cgcaacgctg ttcgtcagca
      781 agctgtgggg tgtttccgcg gtcacagccg ccacgtttgt tacgacgctg gtggtgtttt
      841 tgtttgccga aatgcttcca aaacgcatcg caaaagccat ttccgaaccg tacgcgctgc
      901 tggtttcggg cagcctgatc ttctttatga agatcctctc gcccgttgcg cgcgtgtttt
      961 ccgcgctcgg ccggttcgtt ggcaaaccgt tccgcaagca ggaagaaccg ccgaccgtct
     1021 ccgaggacga actctacgac attatagaaa ccgttgcgac cgaaggcgtg atcgacgaag
     1081 agaaaacgga actcgtccag agcgcgctgg aattttcgga tacgtctgcg ttcgacatcc
     1141 tgaccccctg ggatcgggtc gtcaaagtca cgacggatat gactcaggcg caggttctcg
     1201 attcgattaa aaacaacgtc cattcccgcc tgccggtggt gaacgataag aacaagcctg
     1261 tcggcatgct gcagattcgc aagtatctga aggcttacct gaaaggcgcc gtccggctgg
     1321 aagacgttat ggacgactgc cggtttgtca gcgccagcac cgccatagac gacctgctgc
     1381 cgctgatgag cgcgcagcgc acgcaaattg ccatcgttac cggagacgag gaagagattc
     1441 tcggcatcgt ttccgttgag gatattttag aagaactcgt tggcgaaatc tacgacgagg
     1501 acgatacctt tgacgcggga ggtaccgcgt catgacgaac gtcagttttt ggcagtatct
     1561 cgggatcgtt ggcctactcg gccttttagc gtttttttcc ggcagcgaaa ttgcgtttgc
     1621 aaaagcaaac cgcatgcggc ttcgcgcggc gacggaagaa ggcaacgttc tttccaagat
     1681 cgcctactat atttatcagt catacgacaa cgcgctcgtc acgatactga ttggcaacaa
     1741 tctcgtcaac atcgccgcgt cttccgttgc taccgttatc gccatcgcgt tgatgggcga
     1801 cagcggcgcc tgggttgcca cgctggccat gacggtgctc atcatcatat tcggcgagat
     1861 cgtgccgaaa atcgtcgcca gccaagtgcc ggagggattc gccaaagcag tttccattcc
     1921 gctccgcgcg ctcatgacgc tgctcttccc ccttgtctgg gttctctcgc tgctgctcaa
     1981 aggtgttgac cggctgctca aaaagcgcgt gggcgaaacg ccaagcgtaa cggaagaaga
     2041 t
//
LOCUS       DAOMGY010000310         2040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_62655_length_2040_cov_2.709320, whole genome shotgun sequence.
ACCESSION   DAOMGY010000310 DAOMGY010000000
VERSION     DAOMGY010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62655_length_2040_cov_2.709320"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PLR57_05400"
     CDS             complement(<1..846)
                     /locus_tag="PLR57_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQQ40927.1"
                     /translation="MKLPVYALIDLSTRKVTPYEISKAYYAKYIGGKTLGARLLLDLT
                     PAGVDPLGADAVVIVNTSPMNGTGAPSSSRFNMTFKNVLTGGIASSNCGGQFGVMMKR
                     AGFDGVILKGQSETPVTIEIIDGKVSIADASELWGKDAEETQDAFPKIYGKLVIGPAG
                     EHLVRFASAVSGERVAGRCGAGAVLGAKKVKALVAYGTQRPEIYNRKGFNKYVKKWVA
                     FLRNHPMTGEALPNYGSAGLVSKANSTNALPTHNFKYGHTPDAHYVSGETLTDQYLLR
                     NSSCIS"
     gene            complement(843..1265)
                     /locus_tag="PLR57_05405"
     CDS             complement(843..1265)
                     /locus_tag="PLR57_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQQ40928.1"
                     /translation="MPFFITGDCIGCSACAKICPVLAISGEPKQRYEINIRRCVSCGV
                     CGRVCPKNAVLDGHGNPCKSIPRKNWKKPSIDTKLCSACGICVTWCRAEALRISPPAF
                     HGDIHVFAQLHEPAKCVGCALCEENCPLGAITMTEAQA"
     gene            complement(1422..>2040)
                     /locus_tag="PLR57_05410"
     CDS             complement(1422..>2040)
                     /locus_tag="PLR57_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HQQ40929.1"
                     /translation="RGFKENEMCLYLGFTNGEKGFSRNAAKNVHRVCRKFGGMSLTGF
                     VTKGWEKGRFNDPYMRDTMQDFGIVTDTMECSVNWSNMSKVHAEVRAYCKSRPNTICM
                     THMSHVYPQGANLYFIFIIKESDPEAFRAYHAGILSAIQKSGAAMSHHHGIGKMFAPW
                     LEGSLGKQEYDVLRTLKNHFDPDNIMNPGGTLGFDLKESEKRFPN"
BASE COUNT          408 a          556 c          578 g          498 t
ORIGIN      
        1 ggagatacag gaactgtttc gcagcagata ctgatccgtc agcgtttcgc cgcttacata
       61 atgcgcgtcg ggcgtgtggc cgtatttgaa gttgtgcgtg ggcagtgcgt tggtcgagtt
      121 cgccttgctc acaagccccg cgctgccgta attgggcaga gcctcgccgg tcatggggtg
      181 gttgcgcaaa aacgccaccc attttttgac gtacttattg aaaccttttc tgttgtagat
      241 ttccggtctc tgtgtgccgt aggccacgag cgctttgacc ttctttgcgc cgagcaccgc
      301 gcccgcgccg cagcgaccgg ccacgcgttc gccggagacg gcggaagcaa aacgaacgag
      361 atgttcgccc gctgggccga tgaccagttt gccgtaaatc ttgggaaatg catcctgcgt
      421 ttcctctgcg tccttgcccc agagttcgga agcatccgct attgaaactt ttccgtcgat
      481 gatctcgatt gtcactggtg tttcgctctg gcctttgagg ataacgccgt cgaatcccgc
      541 gcgcttcatc attactccaa actggccgcc gcagttgctg ctggcaatgc cgccggtgag
      601 gacgttttta aacgtcatgt tgaacctgct ggaactcggc gcgcctgtgc cgttcatggg
      661 gctggtgttg acaattacaa cagcgtccgc tccgagcgga tcgacgcccg cgggagtcaa
      721 atccagcagc aggcgcgcgc caagcgtctt tccgccgatg tatttggcgt agtaggcctt
      781 actgatctca tagggtgtta cttttcttgt gctcaggtca atgagcgcgt ataccggcag
      841 tttcatgctt gcgcctccgt catcgtaatc gcgccgagcg ggcagttctc ctcacaaagc
      901 gcacagccaa cgcatttggc tggttcgtgc agctgagcga acacgtggat gtcgccgtga
      961 aacgcgggag gggagatgcg cagtgcttca gcgcggcacc aggtaacgca tatgccgcat
     1021 gcgctgcaaa gcttcgtgtc gatcgaaggc tttttccagt tcttgcgcgg aatgcttttg
     1081 cagggattgc cgtgtccgtc caaaaccgcg ttcttcgggc aaacgcgccc gcagacgccg
     1141 catgaaacac agcggcggat attgatttcg tatcgctgct tgggttcgcc ggagattgcg
     1201 agaacaggac aaatttttgc gcaggctgaa cagccaatac aatcgcccgt aataaagaac
     1261 ggcatgtcgg ttctccttcg ttgaaatgat cggaatctga tatgttcatt gtcactttaa
     1321 cacaagtatt gttgcggcgc aataaccagt gcagacgata aaacaaacgc gttccggcca
     1381 acaagacaag ccggaacgcg gacaatctga aaagtgaatc atcagttcgg gaaacgcttt
     1441 tcgctttcct ttaagtcaaa gccaagcgtg ccgccggggt tcatgatgtt gtccgggtca
     1501 aagtgattct tgagcgtgcg gagcacgtcg tattcctgtt tgcccagcga accttctagc
     1561 cacggcgcga acatcttgcc gatgccgtgg tgatggctca tggcggcgcc ggatttctgg
     1621 attgcggaaa gaatccccgc gtggtacgcg cggaacgctt caggatcgct ctccttgatg
     1681 ataaagataa agtacaggtt cgcgccctgc gggtatacgt gcgacatatg cgtcatgcag
     1741 atcgtgttcg ggcgcgattt gcagtacgct cggacttccg cgtggacttt gctcatgttg
     1801 ctccagttga cggagcactc cattgtgtcg gtcacaatgc cgaaatcctg catggtatcc
     1861 cgcatgtagg gatcgttgaa gcgtcctttc tcccagcctt tggtgacaaa gcccgtcagg
     1921 ctcatgccgc cgaacttgcg gcagacgcga tgtacatttt ttgcggcgtt gcgggagaat
     1981 cccttttcgc cgttcgtaaa gcccagatag aggcacattt cgttttcttt aaagccgcgc
//
LOCUS       DAOMGY010000311         2037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_62804_length_2037_cov_3.212916, whole genome shotgun sequence.
ACCESSION   DAOMGY010000311 DAOMGY010000000
VERSION     DAOMGY010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62804_length_2037_cov_3.212916"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..944
                     /gene="moaC"
                     /locus_tag="PLR57_05415"
     CDS             444..944
                     /gene="moaC"
                     /locus_tag="PLR57_05415"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011333580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HQQ40930.1"
                     /translation="MSETLTHLDEHGAARMVDVSEKDVTHREATAESFVVMKPETLEL
                     IVSGTMPKGDVFATARIAGILAAKRTSELIPLCHPLALTSVRVEPVACPPDRVRITAE
                     VRCDEKTGVEMEALTAASVAALTIYDMCKAVDRGMEIASVRLLKKSGGKSGLFEREGE
                     PCEPNG"
     gene            926..1348
                     /locus_tag="PLR57_05420"
     CDS             926..1348
                     /locus_tag="PLR57_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40931.1"
                     /translation="MRAERLTRAAEKGARDMVGKLTLDAKEGVLGDYKSAQDGSVSLL
                     SAEAEREIRQSGGLCTKKFTANIVTSGLDYAVLSVGTRLGAGDGCVLEITRVGKPCYD
                     ECAIHRENAECPLPKNCAFACIRQGGILRAGDEITLLD"
     gene            1379..>2037
                     /locus_tag="PLR57_05425"
     CDS             1379..>2037
                     /locus_tag="PLR57_05425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ40932.1"
                     /translation="MNDRFGRTIEYLRLSLTERCTLKCSYCRAGEGVCPKQSELTSEE
                     FLRIVRACAQLGVKKVRLTGGEPMLRRDLIEIISGIRSIKGIREITMTTNAQHLPGQS
                     AALKAAGLDRINISVDSLRPERYAEITGGGSLEKVLTGIEEALAAGFSPIKLNAVLLR
                     GVNDDEADDFIALTKDRPIHIRFIEYMPMGDSDQADLRVTGDELLAARPPTGLTISPH
                     LS"
BASE COUNT          445 a          589 c          647 g          356 t
ORIGIN      
        1 ctacgatctg ttggtctcgc ttgcggccat ggagagcccg caggtgcgcg cgtttctgcg
       61 cgtgctgggc tgcgaagcgt ttgcaaagcg gctggaaaag ctcggcgggt acgaactcga
      121 ccgccccgga cgcatccgca agctctggcc ggaaaaggaa gcgtaagcga ccgcgttttt
      181 ggacgttcga ccagaaaaaa cgcttgctct tgccaaaaat tgtttgccgc cgtatgatag
      241 cagtacgagc atgaagagat acgggggctc cgcccccaac ccctggctca ggggtttccc
      301 cctgagaacc ccacgcgtat gggattctta agggatttgt cccttaagca ggtgctcgag
      361 ggcggagccc tcgaaataca taagatcagg atgagggcgg agccctcgac agccaaagaa
      421 taacgaaagg acgtggcggc gaaatgagcg aaacgctgac ccatctggac gagcacggcg
      481 ccgctcgcat ggtggacgtc tccgaaaagg acgtcaccca ccgtgaagcg acggccgaga
      541 gctttgtggt gatgaaaccc gaaacgctgg agctcatcgt ttccggcact atgcccaaag
      601 gagacgtgtt cgccacggcg cgcattgcgg gaattctcgc ggcaaagcgc acgagcgaac
      661 tgattccgct ctgccacccg ctggcgctga cgtccgtccg cgtagaaccc gtggcctgtc
      721 cgccggatcg ggtgcggatt accgccgagg tgcgctgcga cgaaaagacc ggcgtggaga
      781 tggaggcgct gaccgcagca agcgtcgccg cgctgacgat ttacgatatg tgtaaagccg
      841 ttgaccgggg catggagatt gccagcgtgc ggcttttgaa gaagagcgga ggaaagagcg
      901 gcctgttcga gcgcgaaggc gaaccatgcg agccgaacgg ctgacccgcg ccgccgaaaa
      961 aggcgcgcgc gatatggttg gcaagctcac gctggacgca aaagaaggcg tgctgggcga
     1021 ctacaagagc gcacaggacg gttcggtcag cctgctttcc gccgaagcgg aacgggaaat
     1081 ccgccagagc ggcgggctct gcacgaagaa gtttacggcg aacatcgtaa ccagcgggct
     1141 ggactacgca gttctctccg ttggaacgcg tcttggcgcg ggagacggat gcgttctgga
     1201 gattacccgc gtgggcaaac cttgctacga cgaatgcgcc atccaccgcg agaacgcgga
     1261 gtgccctttg ccgaagaact gtgcctttgc ctgcatcagg cagggcggaa tcctgcgggc
     1321 gggcgacgag attactttgt tggactgaat ttatatatca gaaagattac taaaccggat
     1381 gaacgatcgc tttggacgaa ccatcgaata cctccgcctg tcgctgacgg agcgctgcac
     1441 gctcaagtgc tcctattgcc gcgcgggcga aggcgtctgc cccaagcaga gcgaactgac
     1501 aagcgaggaa tttctgcgca tcgtacgcgc ctgcgcccag cttggcgtca aaaaagtgcg
     1561 ccttaccggc ggagagccga tgctgcggcg cgacttaatc gagatcatct ccggaatccg
     1621 ttcaattaaa ggcatccgtg agattacgat gacaaccaac gcccagcatc tgcccggaca
     1681 gtcggcggcg ctgaaggccg cggggctcga ccgcatcaac atcagcgtgg attcgctccg
     1741 ccccgaacgg tacgccgaga ttaccggcgg cggttcgctg gaaaaggtgc tcaccggcat
     1801 tgaggaagcg cttgcggcag ggttttcgcc catcaagctt aacgcggttt tactgcgcgg
     1861 tgtcaacgac gacgaagcgg atgactttat cgcgctcacc aaggacaggc cgatccatat
     1921 ccgctttatc gagtatatgc ccatggggga cagcgatcag gctgacctcc gcgtgaccgg
     1981 ggacgagctg ctcgccgcgc ggccaccgac gggcttgacg atctcgccgc atctctc
//
LOCUS       DAOMGY010000312         2036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_62848_length_2036_cov_3.070167, whole genome shotgun sequence.
ACCESSION   DAOMGY010000312 DAOMGY010000000
VERSION     DAOMGY010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62848_length_2036_cov_3.070167"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLR57_05430"
     CDS             complement(<1..612)
                     /locus_tag="PLR57_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQQ40933.1"
                     /translation="MLRFPFAGIALSDTNRYAEGEGERAPLISSIRWYIETEKRTVQR
                     NPAVLYALDAAALYALNIPVSKLLLVGVSPAMLAAFLYLGAGAGVGLLRLIGKRMRKE
                     TADQPLTRHELPYTLAMIVLDILAPILLMLGLKTTAADTASLLGSFEIVATAILAMIL
                     FSEKVSGRLWIAIALITLASLVLSLEGTARFSFSPGAVLVLAAC"
     gene            complement(618..>2036)
                     /locus_tag="PLR57_05435"
     CDS             complement(618..>2036)
                     /locus_tag="PLR57_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQQ40934.1"
                     /translation="DPQVVMAPPGEEFVRAAAADAYDRLILPSMERETRGTMTDMACE
                     GAIHNFAINLKPLLMQPPVKGHVTMGLDPGYRNGCKVAVVSATGKVLDTAVVYPTFGE
                     RQKNEAIAALEALVKKHHVEHIAIGNGTASRETEQTTCELIAKTPGVSYMIVSEAGAS
                     VYSASKLAAEEFPQYDVNLRSAVSIARRLQDPLAELVKIDPKAIGVGQYQHDMPQKRL
                     SETLEGVVEDCVNAVGVDVNTASPSLLKQVAGLNETLAKNIVAYREENGAFTSRKQLL
                     KVPKMGPKAFEQCAGFLRVPESKNVLDHTGVHPESYAAAEKLLSLCGYSLADVAAGRI
                     ADLSERVKQFGESKAAEYCGVGLPTLRDVVKELTKPGRDPRDELPKPVLRTDVLDIKD
                     IVPGMILTGTVRNVIDFGAFVDIGVHQDGLVHISEIANRFVKHPSEAVKIGDVVKVLV
                     LGVDPVKKRISLSIKQAAEKQN"
BASE COUNT          406 a          633 c          586 g          411 t
ORIGIN      
        1 gcaggcggca agcaccagca ccgcacccgg cgaaaacgaa aaccgcgccg taccttccag
       61 cgaaagaacg aggcttgcca gtgtaatcag cgcgattgca atccagagtc gcccagaaac
      121 cttctcggaa aagagaatca tcgccaagat tgcggtggca acgatttcga aactgccgag
      181 cagcgaagcg gtatctgcgg cggtggtctt tagtcccagc atgagcagaa tcggcgcgag
      241 gatatccagc acaatcatcg cgagcgtata cggaagttcg tgccttgtca gcggctgatc
      301 ggcggtttct tttcgcatgc gtttaccgat caggcgaagg agccccaccc ccgcgcccgc
      361 gccgaggtag aggaacgccg cgagcatggc gggcgaaaca ccaaccagca gaagtttgga
      421 aacgggaata ttgagcgcat agagtgccgc cgcgtccagc gcgtataaaa cggcaggatt
      481 tcgttgtact gttcgctttt ccgtttctat ataccacctt atagaagaaa tcaggggagc
      541 gcgttcgccc tccccttcag cataccgatt tgtgtctgaa agcgcaatcc ccgcaaaggg
      601 aaagcgcagc acatgcctta attctgcttt tctgcggcct gcttgatcga aagcgaaatg
      661 cgctttttca ccggatcgac gcccagcacg agcaccttga ccacgtcccc gattttgacc
      721 gcctcggacg gatgcttgac gaagcggttg gcgatttccg aaatatggac gagcccatcc
      781 tgatgcacgc cgatatccac gaacgcgccg aagtcgatga cgtttcgcac cgtgccggtg
      841 aggatcatgc cgggcacgat gtccttgatg tccagcacgt ccgtacgcag aacgggcttg
      901 ggcagctcgt cgcgcgggtc tctgccgggc ttggtcagtt ccttgacgac gtcccgcaga
      961 gtcggcaggc caacgccgca atattcagcc gctttcgact cgccaaactg cttaacgcgc
     1021 tcgctgaggt cggcaattcg acccgccgcc acatccgcaa gcgaatagcc gcagagcgag
     1081 agcagctttt ccgccgcggc gtagctctcc gggtgtacgc cagtgtgatc gagaacattt
     1141 ttgctctccg gcacgcgcaa aaagcccgcg cactgctcaa aggctttcgg ccccatcttg
     1201 ggcactttca agagctgctt gcgggaggta aacgcgccgt tttcctcgcg gtaggcgaca
     1261 atattctttg ccagcgtttc gttaagaccc gccacctgct tgagcagcga aggcgacgcg
     1321 gtgttgacgt ccacgcccac ggcgttgacg cagtcctcca ccacgccttc gagcgtctcg
     1381 ctgaggcgct tctgcggcat gtcgtgctga tactggccta cgcctatggc cttggggtcg
     1441 attttgacca gctccgccag cggatcctgc agcctccgcg caatggagac ggcggaacgc
     1501 aggtttacgt cgtactgcgg aaactcctcg gcggcgagct tgctggcaga atacacggaa
     1561 gcgcccgcct cgctgacgat catgtagctg acccccggcg tttttgcgat cagttcgcaa
     1621 gtcgtctgct cagtttcgcg gctggccgtg ccgttgccga tggcgatatg ctccacatgg
     1681 tgtttcttta ccagcgcttc cagcgcggca attgcttcgt ttttctgccg ctcgccgaag
     1741 gtcggataca caacggcggt atccagaacc ttgccggtcg cgctgacaac ggccaccttg
     1801 cagccattgc ggtagcccgg gtcgagaccc atggtcacat gccccttgac cggcggctgc
     1861 atcaaaagcg gttttaagtt gatggcgaag ttgtgaatcg cgccctcgca ggccatatcc
     1921 gtcatcgtgc cgcgcgtttc gcgctccatg gaaggcagaa tcagccggtc gtatgcatcc
     1981 gcagccgccg cgcggacgaa ctcctcgccg ggcggcgcca tgaccacctg cggatc
//
LOCUS       DAOMGY010000313         2031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_63083_length_2031_cov_3.868421, whole genome shotgun sequence.
ACCESSION   DAOMGY010000313 DAOMGY010000000
VERSION     DAOMGY010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63083_length_2031_cov_3.868421"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLR57_05440"
     CDS             <1..818
                     /locus_tag="PLR57_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HQQ40935.1"
                     /translation="AGEKVNMFDINAVSKTVNFPGSATQDDVRSVYLLAYQEGLKGVT
                     IYRDGSRTGQVLNIGSVNNGKTDGQKPADGADETALPPDYGHIEPRPRPVVTRGFTEK
                     MNIGCGTLYVTVNYDENGICEVFTSTGKAGGCPSQSEATARLTSIALRSGISITEVYD
                     QLKGIRCPSTIRQKNMSCTSCPDAIARVLMKVSKYIDEGAEHGTPEAQQKTQTPAART
                     ANGAETEIPFIPRDNGEDKPSEKKSGACPECGGRVEHEGGCMVCRACGFSKCG"
     gene            866..1990
                     /locus_tag="PLR57_05445"
     CDS             866..1990
                     /locus_tag="PLR57_05445"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HQQ40936.1"
                     /translation="MKAVLLTCSTGQGHNSAALAVKQALEQRGAECDIQDALAFLGEN
                     VSKTIEEAFVNIAVKTPRAFGFMYAAGEFLSSERRKSPVYFANALYAENLRRYLVENE
                     IDAAVCPHLFPAEALTYLRKKQHLSARTYYISTDYACIPFLEETDMDAVFTPHPDLSD
                     AFVKRGIPAELLLTSGVPVDQAFTAHVEKADARLTLDLPVDLPCYLVMTGGEGCGDAQ
                     TLTKKLLERLKGRDARIIVLTGHNGQLLRSLESRFGGDVRVLAVPFTNQVPLYMDACD
                     VLLTKPGGISSTEAAVKGIPMVHTPPIPGVETLNAQFFSERGMSVFAPNEDSAAENAI
                     RLAMDLPQRSRMLAAQKQYLSAAAADRIAACILGQSTELQ"
BASE COUNT          456 a          606 c          607 g          362 t
ORIGIN      
        1 ttgcaggaga aaaagttaac atgttcgata tcaacgcggt ttccaaaacc gtcaacttcc
       61 ccggcagcgc cacgcaggac gacgttcgct cggtgtacct gcttgcgtat caggaagggc
      121 tcaagggcgt aacgatctac cgcgatggca gccgcaccgg ccaggtgctc aacatcggct
      181 ccgtcaacaa cggaaaaacc gacgggcaga agcctgccga cggcgcggac gaaaccgctc
      241 tgccgccgga ttacggccat atcgagccgc gcccgcgccc cgttgtgacg agaggcttca
      301 ccgaaaagat gaacatcggc tgcggcacgc tgtatgtaac cgtcaactac gacgaaaacg
      361 gcatctgcga agtcttcacc agcaccggca aggcgggcgg ttgtcccagt cagagcgagg
      421 cgaccgcgcg gcttacatcc attgcgctga gaagcggcat atccattacg gaagtgtacg
      481 accagctcaa aggcatccgc tgcccctcga ccatacggca gaagaacatg agctgcacct
      541 cctgcccgga cgcgattgcc cgcgttctta tgaaagtcag taaatacatc gacgaaggcg
      601 cggagcacgg cacgccggaa gcgcagcaaa agacgcagac gcccgcggcg cgcacggcaa
      661 acggcgcgga aacggagata ccgttcattc cgcgcgacaa cggagaagat aagccgtccg
      721 aaaagaagag cggcgcctgc ccggagtgcg gcggacgcgt tgagcacgaa ggcggctgca
      781 tggtctgccg cgcatgcggc ttcagtaaat gcggctgagc ccggcgtttg ggatgcgccg
      841 gccggtttag cggaaggatg ggagcatgaa agcggtactc ttaacctgca gcactgggca
      901 gggacataac tccgccgcgc tggcggtcaa acaggcgctg gaacagcgcg gcgcggagtg
      961 cgatattcag gatgcgcttg cgttccttgg cgaaaatgta tcaaaaacca tcgaagaagc
     1021 gttcgtcaac attgcggtca aaacgccgcg cgcgttcgga ttcatgtatg cggcgggaga
     1081 attcttaagt tccgagcgca gaaagtctcc agtttatttt gccaacgcgc tgtatgcgga
     1141 aaacctccgc cggtaccttg tggaaaacga aatcgacgcg gcggtctgcc cgcatctctt
     1201 cccggcggaa gcgctgacct atcttcgcaa aaagcagcat ctctccgccc gaacgtatta
     1261 tatctccacc gactatgcct gcattccgtt tctggaggag acggatatgg acgcggtgtt
     1321 tacgccgcat cccgacctgt cggacgcctt tgtcaagcgc ggaatccccg ccgaactgct
     1381 gctgacaagc ggagtgcccg tggatcaggc atttaccgcg cacgtggaaa aggcggacgc
     1441 ccgcctgacg ctcgacctgc cggtcgatct gccctgctat ctcgtaatga ccggcggcga
     1501 aggctgcggg gacgcgcaga cgctcacgaa aaagctgctg gagcggctca agggcaggga
     1561 cgcgcgcatc atcgttttga ccgggcacaa cgggcagctg ctgcggtcgc ttgaaagccg
     1621 gttcggcggg gacgtccgcg tgcttgcggt tccgtttacg aatcaggttc cactatatat
     1681 ggacgcctgc gacgtgctcc taaccaaacc cggcggcatc tccagcacgg aagcggccgt
     1741 caaaggcatc cctatggtgc atacgccgcc aataccgggc gttgaaacgc tcaacgcgca
     1801 gtttttcagc gagcggggca tgtccgtttt cgcaccaaat gaagactccg ccgcggagaa
     1861 cgcaatccgg cttgctatgg atcttcccca gcggtcgagg atgctcgccg cgcagaaaca
     1921 gtacctttcc gctgcagccg cagacaggat tgccgcgtgt attctcggtc aatcgaccga
     1981 gttgcagtaa aactgaacgt cgagtacgtt tttcacaatt catggctttt t
//
LOCUS       DAOMGY010000314         2031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_63106_length_2031_cov_2.636134, whole genome shotgun sequence.
ACCESSION   DAOMGY010000314 DAOMGY010000000
VERSION     DAOMGY010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63106_length_2031_cov_2.636134"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="PLR57_05450"
     CDS             <1..1342
                     /locus_tag="PLR57_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQQ40937.1"
                     /translation="APENENHPSWKMTVRHLLNMSTGSFFNEAGSAMGSDWVKDFLHT
                     GVKFPAGSAFDYNSMNTYMLAAIIRRKTGQTLTEYLTPRLYEPLGIEAHPWETCPNGT
                     EKGGWGLSLTMESATKIGQLYLNKGRWETETGVRQLVSERWVEEATRPQIDTPKGEIT
                     YGYGHQIWMTARKGAFLFNGAFGQYMLALPDRNALVVLFSGTSRLFANGGVLDQVTAA
                     LDTVQDPPLPPLPKDEAGREALATTLNGLTVRYRKPFFNPNLRALPLEEAARRLDGRV
                     YTFPANIGGIMPMILQSVHNNFSAGLTRAAFDQLEDGGLLVELAEGEATHRFVLADNR
                     YTAGTVCQRGDTYAIRSAAQAALLDGDILQINAVVHFIETPFTRLVKFVFTQDKLKIE
                     FDESPSIRDASEMMLELTGITRVQVVRNLMPLLKRDRLQHTLRTFTTVTVQGTL"
     gene            complement(1553..>2031)
                     /locus_tag="PLR57_05455"
     CDS             complement(1553..>2031)
                     /locus_tag="PLR57_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HQQ40938.1"
                     /translation="FCADVQSNVDKDVGEVLKHILPEDLLKFGLIPEFVGRMPIIVTL
                     ENLDEDALVRILTEPKNALTRQYQRLFEMDGVELTFDEDAIRAVAKKAIERKTGARGL
                     RAILEDVMLDIMFDIPSREDVATCRITADVIEKVCPPLLAEGTGEKRALKKPKQSA"
BASE COUNT          408 a          614 c          612 g          397 t
ORIGIN      
        1 agcgccggaa aacgaaaacc acccttcctg gaagatgacc gtgcgccact tactgaacat
       61 gagcaccggc tctttcttca acgaagcggg ttccgccatg ggctcggact gggtcaagga
      121 ctttctgcac acgggggtca agttcccggc tgggtcggct tttgactata actcgatgaa
      181 cacctacatg ctcgcggcca tcatccgccg taaaacgggt caaacgctca ccgagtattt
      241 aacaccccgc ctatacgaac cgctcggcat cgaggcgcac ccctgggaaa cctgccccaa
      301 cggcacggaa aagggcggct gggggctttc gctgaccatg gagagcgcga caaagatcgg
      361 ccagctctac ctcaacaaag gccgctggga aacggaaacc ggtgtgcgcc agcttgtcag
      421 cgaacgctgg gtggaggagg ccacccgccc gcagatcgat acgccaaagg gtgaaatcac
      481 ctatggctac ggccaccaga tttggatgac cgcgcgtaaa ggcgcgttcc tctttaacgg
      541 tgcgttcggg cagtacatgc ttgccctgcc ggatcgaaac gcgctggttg tgctgttttc
      601 cggcaccagc cggttgtttg ccaacggcgg tgtgctcgat caggtgaccg ccgcgctgga
      661 taccgtgcag gatccgccgc tgccgccgct gccaaaggac gaggccgggc gcgaagcgct
      721 ggctactacg ctcaacggcc tgacggtgcg ctaccgcaag ccattcttca acccgaatct
      781 ccgcgccttg ccgctggagg aagctgcgcg gcggctggac gggcgcgtat ataccttccc
      841 tgcaaacatc ggtggaatta tgcctatgat tctgcagagc gtgcacaata acttttccgc
      901 ggggctgacg cgcgcggcgt tcgaccaact cgaggacggc gggcttctgg tggagcttgc
      961 ggaaggggag gcaacgcatc gctttgtgct agcggataac cgctacacgg cgggaaccgt
     1021 ctgccagcgc ggcgatactt acgccatccg atccgcggcg caggcggcgc tgctggacgg
     1081 cgacattctg cagatcaacg cggtggtgca ttttatcgaa acaccgttca cgcggctcgt
     1141 gaagttcgtg ttcacgcagg acaagctgaa gatcgagttc gacgaaagcc cgtccattcg
     1201 ggacgcttcc gagatgatgc tcgagctcac cggcatcacc cgcgtgcagg ttgtccgcaa
     1261 cctcatgccg ctgctcaagc gcgacaggct ccagcatacg ttgaggacgt ttacgactgt
     1321 taccgtacag ggaacgctgt aaattggggc tttacgaggg ctctgccctc gcgctcccgc
     1381 caaggggcaa gccccttgga accccttatc tatgggagtc caaagggaga atccctttgg
     1441 ttggggtttg gggctaagcc ccaagaataa tcgtatatgg ggcgaagccc gaagaaaagg
     1501 caaaacaaaa acgagcggaa cctcacggtt ccgctcgttt gttgtgctta ctttacgccg
     1561 actgtttggg tttcttcagc gcccgttttt cgccggtgcc ttccgcaaga agcggcgggc
     1621 agaccttttc gattacgtcc gcggtgatgc ggcaggtggc aacgtcctcg cgggagggga
     1681 tgtcgaacat gatgtccagc atcacgtcct ccagaatcgc ccgtaagccg cgcgcgccgg
     1741 ttttgcgctc gatggccttc ttggcaacgg cgcggatggc gtcctcgtcg aacgtcagct
     1801 cgacgccgtc catctcgaac agccgctgat actggcgggt gagggcgttt ttcggctcgg
     1861 tcaggatgcg aaccagcgcg tcctcgtcca gattctcgag cgttacgata atcggcatac
     1921 gcccgacaaa ctccggaatc aggccgaact tgagcaggtc ttccggcagg atgtgcttga
     1981 gcacctcgcc gacgtcctta tccacgttgc tctgcacgtc cgcgcaaaag c
//
LOCUS       DAOMGY010000315         2027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_63290_length_2027_cov_3.316937, whole genome shotgun sequence.
ACCESSION   DAOMGY010000315 DAOMGY010000000
VERSION     DAOMGY010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63290_length_2027_cov_3.316937"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /gene="trmD"
                     /locus_tag="PLR57_05460"
     CDS             complement(<1..549)
                     /gene="trmD"
                     /locus_tag="PLR57_05460"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQQ40939.1"
                     /translation="MRIDILTIFPEMFEGVLGSSMLGRARQNGLIDIRVHNIRDYTDN
                     KHRKTDDYPFGGGAGMVMMAQPIFDCVDAVLGGEQARRILLSPRGRTLNQKIAKELAG
                     EERLLLLCGHYEGVDERVMSIMDEELSIGDYVLTGGELPAMVLTDCLSRFIPGVLGSE
                     ESAADESFSDGLLEYPQYTRPAE"
     gene            complement(539..1057)
                     /gene="rimM"
                     /locus_tag="PLR57_05465"
     CDS             complement(539..1057)
                     /gene="rimM"
                     /locus_tag="PLR57_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060099.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQQ40940.1"
                     /translation="MKNKYLRVGQIVRAHGVHGDVKVLPLTDDPARYRKLKTAYLERG
                     GDYLPVALDDVRLQPDAVILHVSGYDTPEKAEQLKSAYLCVDRENAVALGEYTYFVAD
                     LIGCETFDTAGKAYGKITDVLETGANDVYEIEHGKLLVPALKRVLDSVDIANGRIVFN
                     ADVLEEVGCFAD"
     gene            complement(1047..1280)
                     /locus_tag="PLR57_05470"
     CDS             complement(1047..1280)
                     /locus_tag="PLR57_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HQQ40941.1"
                     /translation="MEELVRYIAQNLVDEPEAVTVESREEGDTVIISLSVAPSDMGKV
                     IGRQGRIAKAIRTVVKAASVREEKKYMVDIVEK"
     gene            complement(1292..1534)
                     /gene="rpsP"
                     /locus_tag="PLR57_05475"
     CDS             complement(1292..1534)
                     /gene="rpsP"
                     /locus_tag="PLR57_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQQ40942.1"
                     /translation="MLKIRLRRMGAKKAPFYRIVVADSRAPRDGACVEEIGYYNPLSN
                     PVELNVNNEKAAQWVKQGAQPTDTVRALLKKSGAIE"
     gene            complement(1616..>2027)
                     /locus_tag="PLR57_05480"
     CDS             complement(1616..>2027)
                     /locus_tag="PLR57_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017796645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQQ40943.1"
                     /translation="ANFDERKAVEMAEKMKTNSFTLDDFLDQMEQVRSMGSMEDILKM
                     IPGMDAGKLGGAQIDEKQMGRTKAIIQSMTKAERANPDILNASRRKRVARGSGTSVQE
                     VNRLINQFNASRQMMKQMSGRMKKGKRGGFPFGF"
BASE COUNT          359 a          634 c          560 g          474 t
ORIGIN      
        1 ctccgcaggc cgcgtatact gcgggtattc cagcaggccg tcggaaaacg actcgtccgc
       61 ggcgctctct tcgctgccca gcacgcccgg aatgaaccgg ctgaggcagt cggtcagcac
      121 catggcgggc agctcgccgc cggtgagcac atagtcgcca atggagagct cttcgtccat
      181 aatgctcatc acgcgctcgt ccacgccttc gtagtgcccg cagagcagca aaagccgctc
      241 ttcaccggcg agttccttgg caatcttctg attcagcgtc ctgccgcgcg gggaaagcaa
      301 aatccgtcgc gcttgttcgc cgcccagcac cgcgtccacg cagtcgaaga tgggctgcgc
      361 catcatcacc atgcccgcgc cgccgccaaa ggggtagtcg tcggtttttc tgtgcttgtt
      421 gtccgtatag tcgcggatgt tgtgcacgcg gatatcgatt aagccgttct ggcgcgcgcg
      481 tcccagcatg ctggaaccca gcacgccttc aaacatctcc ggaaagatcg tgagaatgtc
      541 aatccgcaaa gcagccgacc tcctcaagta cgtccgcatt gaacacgatg cgcccgtttg
      601 caatgtccac gctgtcgagt acgcgcttga gcgcgggcac cagcagtttt ccgtgctcaa
      661 tttcgtaaac gtcgttcgcg ccggtctcca gcacgtcggt aatcttaccg taggctttgc
      721 ccgccgtatc gaacgtttcg cagccgatca ggtcggcaac gaaatacgtg tattcaccca
      781 gcgcaacagc gttttcgcgg tccacgcaga ggtacgcgct tttcagctgt tccgctttct
      841 ccggcgtgtc gtaccccgaa acatgcaaaa taaccgcgtc cggctgaagc cggacgtcgt
      901 ccagcgcgac gggcaagtag tcgcccccgc gctccagata tgcggttttc agtttgcggt
      961 agcgggccgg atcgtccgtt agcgggagca cttttacgtc cccgtgcacg ccgtgtgcac
     1021 ggacgatctg accgacgcgc aggtacttat ttttcaacga tatcgaccat gtacttcttt
     1081 tcttcgcgga cgctggcggc cttgacgacc gtacggatcg ctttggcgat gcgtccctgc
     1141 ctgccgataa ccttgcccat gtcggaggga gcgacggaaa gggaaatgat gaccgtatcc
     1201 ccttcctcgc gcgattccac cgtcacggct tccggctcgt ctacgaggtt ctgcgcgatg
     1261 tagcggacca gctcctccat cggttccatc ctcactcgat ggcgccgctc ttcttgagca
     1321 gagcgcgtac cgtgtcggtc ggctgtgcac cctgcttgac ccactgagcc gctttttcgt
     1381 tgttaacgtt cagctctacc gggttcgaaa gcgggttgta gtaaccgatt tcttctacgc
     1441 aggcgccgtc gcggggcgcg cgggagtccg caactacgat acggtagaaa ggtgctttct
     1501 tggcgcccat gcggcgcagt ctgatcttga gcattgtctg aatcctccat atatgaatgt
     1561 tttttatatc gaatacgtat aaacgtgatt cgccgtgtat aagggcgcta acagcctaga
     1621 acccgaacgg aaagccgccg cgcttgcctt ttttcatgcg cccgctcatc tgcttcatca
     1681 tctgccggga agcgttgaac tggttgatca ggcggttgac ttcctgcacg ctcgtgccgc
     1741 tgccgcgcgc gacgcgtttt ctgcggctgg cgttgagaat gtcggggttc gcccgttccg
     1801 ccttggtcat ggactggata atcgcctttg tgcgccccat ctgcttttcg tcgatctgcg
     1861 cgccgccgag tttaccggcg tccatgccgg ggatcatctt gaggatgtcc tccatggaac
     1921 ccatggagcg aacctgctcc atctgatcca gaaaatcgtc cagcgtgaac gagttggtct
     1981 tcatcttctc ggccatctcg accgcttttc tctcatcaaa gttggct
//
LOCUS       DAOMGY010000316         2025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_63384_length_2025_cov_3.125381, whole genome shotgun sequence.
ACCESSION   DAOMGY010000316 DAOMGY010000000
VERSION     DAOMGY010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63384_length_2025_cov_3.125381"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1386)
                     /locus_tag="PLR57_05485"
     CDS             complement(154..1386)
                     /locus_tag="PLR57_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U34"
                     /protein_id="HQQ40944.1"
                     /translation="MCDTLGVLFDGHSLFGKNSDRSPNEAQVTEFYPARVCKETSLSA
                     TYIAIPQARETKAVLLSRPVWMWGAEIGVNEAGVAIGNEAVFTKGPYGKPALTGMDLL
                     RLALERSESAESAVNTIIELLETYGQGGNCGFDHTFYYDNSFLVIDRDHLFVLETCAK
                     SWVVKSYRKASISNRLSIGADGDRYGGGACDFAKVHTDPLYTFFSGSKLRQGQSGCAL
                     ETIESPVGMASALRRHNPSVKNPFAEGSVSSCCMHYGGAVGDQSTASMIVSLSGGAPT
                     VFLSGTSLPCVSLLKPYRFGNPARLPVSSLGESAAEAYWRSAESYRRSLIGKVVPAEF
                     YAERDALEQSWMADVSADDSAMDELLLRALREEAAFYAKFDPQSFPAVPVKKAFSGNS
                     KKKNSAFAALAPKEEARA"
     gene            1584..>2025
                     /locus_tag="PLR57_05490"
     CDS             1584..>2025
                     /locus_tag="PLR57_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQQ40945.1"
                     /translation="MELDPLAIRNLLIWIALALGNTIFLGATPNWMRCGAKRADPSAA
                     SALFALVFAGASAAFLAFTGGAGALLTLSVQQYLILAACGLLSALTWLSLFTAMTGGR
                     VNKVIPVYLLGSIFTLTASHFLFGAPLGLWKICCVIVILLGIVFI"
BASE COUNT          368 a          593 c          628 g          436 t
ORIGIN      
        1 cgccgttttg tagggctact tcgcggatgt tgccgtagca gaactgctcg ccggcgctta
       61 gcccgccgta ggaaacggcg atgccgctga tgacgcgccg ttcgtcaacc agatattgta
      121 tgcccgaatc gagcgtttcg cggggagata ccgtcatgcg cgcgcttcct ccttgggagc
      181 gagcgcggca aacgcgctgt ttttcttttt cgagttgccg gaaaacgcct ttttcaccgg
      241 cactgcggga aagctctgcg ggtcgaactt tgcgtagaac gccgcttctt cgcggagcgc
      301 gcgcaggagc agttcatcca tcgcgctgtc gtctgcggag acgtccgcca tccagctttg
      361 ctccagcgcg tcgcgctcgg cgtagaattc ggcgggaacg actttgccga tgagcgatct
      421 gcggtaactt tcggcgcttc tccagtacgc ttccgccgcg ctctcgccaa ggctcgaaac
      481 gggcaggcgc gcgggattgc cgaagcggta aggcttaaga agcgaaacgc agggaagcga
      541 cgtgcccgaa agaaagaccg tcggcgcgcc gccggaaagc gaaacgatca tgctcgccgt
      601 cgactggtcg ccgaccgcgc cgccgtaatg catgcagcag ctcgaaaccg agccctcggc
      661 aaaggggttc ttgacggaag gattgtgcct gcgcagcgcg ctcgccatgc ccacggggga
      721 ttctatggtc tccagcgcgc agccggactg gccctgacgc agtttggagc cggaaaagaa
      781 ggtatacagc gggtcggtgt gtacctttgc aaagtcgcac gctccgccgc cgtagcggtc
      841 gccgtccgcg ccgatgctga ggcggttgct gatggaagcc ttgcggtagg atttgaccac
      901 ccagcttttt gcgcaggttt caagcacaaa caggtgatcg cggtcgatca cgagaaacga
      961 gttgtcgtaa taaaacgtat ggtcgaaccc gcagttgccg ccctgaccgt aagtttccag
     1021 aagctcgatg atggtattga cggcgctttc cgcgctctcg gaacgctcca gcgcgagccg
     1081 aagcaggtcc atacccgtga gcgcgggctt gccgtaaggc cccttggtaa acaccgcttc
     1141 gttgcctatg gcgacgcccg cttcgttgac gccaatctcc gcgccccaca tccaaacagg
     1201 ccgggagagc agcaccgcct tggtttcccg cgcttgcgga atggcgatat aggttgccga
     1261 aaggctcgtc tccttgcaga cccgcgcggg ataaaactcc gttacctgcg cttcgttggg
     1321 gctgcggtcg ctgtttttgc caaagagcga atggccatca aataaaacgc cgagcgtatc
     1381 gcacatgctg cttatcctcc ggactttttt tccagtatag catagttttt caggtgcagg
     1441 aaccgtgcgg tgcgggcaaa aaaagccgtc tgggcgcatg cggaaccgtt cgccggagtt
     1501 tgccgcgcac acaaggcgaa aaggttgtga aacgggcggt tcgtatggta caatacgtgg
     1561 gcaaacaggc agggaggcaa cggatggaac ttgacccgct ggcaatccgc aatctgctga
     1621 tttggattgc gcttgcgctt ggcaatacaa tttttctggg agctactcca aactggatgc
     1681 gctgcggcgc aaagcgcgcg gacccttccg cggcgtctgc gctgttcgcg ctcgtgttcg
     1741 cgggcgcttc cgccgcgttt ctggcgttta ccggcggcgc gggcgcgctt ttgacgcttt
     1801 cggttcagca gtatctgatc cttgccgcgt gcgggctgct ctcggcgctg acctggctga
     1861 gcctgtttac ggccatgacg ggcgggcgcg tcaacaaggt gattcccgtg tacctgctgg
     1921 gcagtatatt tacgctgacc gcttcacatt tcctcttcgg tgcgccgctt ggcttatgga
     1981 agatctgctg cgtcatcgta attctgctcg gtatcgtgtt tatcg
//
LOCUS       DAOMGY010000317         2021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_63587_length_2021_cov_3.264496, whole genome shotgun sequence.
ACCESSION   DAOMGY010000317 DAOMGY010000000
VERSION     DAOMGY010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63587_length_2021_cov_3.264496"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1627
                     /locus_tag="PLR57_05495"
     CDS             <1..1627
                     /locus_tag="PLR57_05495"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HQQ40946.1"
                     /translation="EEPKITTAGGTFDSAQQVTIEVPEGCSVSYTTDGTVPTESSSRY
                     SGAITVSKTTILRARSFRSGYYGSDTASQTYIIYTGESTIQSHTYTLPVISIVTDPKN
                     LWDPEIGIYVAGSDYAEATGEGTDPLQTKISPTDPNWNLLNFWAQPKSHPDPLGRKWE
                     RDVHFDYIDQNGTTLYSGDGAIQIQGQFSRFGEQKGLALIARGAYGDSMFNYPFFENR
                     DITSYKSLILRASAQDSTYSHIRDNLVTTLFEEGNTGLPEDRAVIVQAHKQVVVLLDG
                     QYWGVYDLMEKITEHFVAEHFHLSNPQSVDLLFGNGNQNCVLAGGGWEDYTDMVEWAG
                     SHDLSDSANYDYICSLMDTENYATYVAAEIIVGNTDSANIKYWRSTETDNKWRWIFYD
                     FCWAMNRNDDKSDAYSSGYRRDFFSKYFDPEGHGTGKAASTVLIRALLQNASFKEMFL
                     QKVALMLNDVFTPEKINAMVDLLQNNIAEEMKYTVDILDGITYKTWQQHCDHIRTYAD
                     NYQEYALKYVQNYFSLSDTEMVNIFGRVTTLED"
     gene            1642..>2021
                     /locus_tag="PLR57_05500"
     CDS             1642..>2021
                     /locus_tag="PLR57_05500"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HQQ40947.1"
                     /translation="MANKNGLAGKPYRHELKYIISEGEHKLLATRLKACLKQDYYASL
                     NGGEYFIRSLYFDDPFDSAVAEKADGVGSRDKFRIRIYNLSDSTIKLERKHKEGQYIK
                     KDSVSLTREECDAIVRGNLACLLA"
BASE COUNT          520 a          543 c          546 g          412 t
ORIGIN      
        1 cgaggagccg aaaattacga ccgcgggcgg cacgtttgac agcgcccagc aggttaccat
       61 agaagtaccg gaaggctgct cggtgtcgta caccacggac gggacggtgc ccaccgagag
      121 ctcttcgcgc tattccggcg cgattacggt ttccaaaaca actatcctgc gcgcgcgttc
      181 gttccggagc ggttactacg gcagcgacac cgcgagccag acctatatta tttacaccgg
      241 cgaaagcacg attcagagcc atacctatac cctgccggta atcagcatcg taaccgatcc
      301 gaagaacctc tgggacccgg aaatcggcat ctatgttgcc ggcagtgact atgcggaagc
      361 caccggtgaa ggaacggatc cattacagac caagatttcg cccacggatc caaactggaa
      421 tctgctgaat ttctgggcgc agcccaaatc gcatcccgat ccgcttggca gaaaatggga
      481 acgcgacgtg cactttgatt atatcgatca aaacggcacg acactctaca gcggcgacgg
      541 agcgattcag attcagggtc agttttctcg ctttggtgaa caaaagggtc ttgcgctgat
      601 cgcgcgcggc gcgtacggcg actcgatgtt caattacccg ttctttgaaa accgcgacat
      661 cacgagctat aaatcgctga ttcttcgcgc aagcgcacag gattcgactt atagccatat
      721 ccgcgataac ctggtcacta cgctttttga agagggcaat accgggctgc cggaggatcg
      781 cgcggtgatc gtgcaggcgc acaagcaagt cgttgttctc ctcgatggcc aatactgggg
      841 cgtatacgac ttaatggaga agattacgga gcacttcgtt gccgaacact ttcatctgag
      901 caatccgcag tcggtcgacc tgctctttgg caacggaaac cagaactgcg tacttgccgg
      961 cggcggctgg gaagattata ccgacatggt cgagtgggcg ggcagccacg atttgagcga
     1021 ctcggcgaac tacgactaca tttgctcgct catggacacg gagaactacg caacctatgt
     1081 cgcggcggaa atcatcgtag ggaacacgga ttcggcaaac atcaagtact ggcgcagcac
     1141 ggaaaccgat aataaatggc gctggatctt ttatgatttc tgctgggcga tgaaccgcaa
     1201 cgacgataag tcggatgcct actcgagcgg ataccgaagg gactttttct cgaaatattt
     1261 cgacccggaa ggccatggca ccgggaaagc ggcttcaacg gtgctcatcc gcgcgctgct
     1321 gcagaacgcg tcttttaaag agatgttcct ccagaaagtg gcgctgatgc tcaacgacgt
     1381 gtttacgccg gagaagatca acgcgatggt agatctcttg caaaacaaca ttgccgagga
     1441 aatgaagtat accgttgata ttctggacgg catcacctat aagacctggc agcagcactg
     1501 cgaccacatc cgcacatatg cggacaatta tcaggaatat gcgctgaaat atgtgcagaa
     1561 ttacttcagc ctgagcgata cggaaatggt gaacattttc gggcgcgtta ctacgctgga
     1621 agactaggaa aggcttggat catggccaat aaaaacggac ttgccggcaa gccttaccgg
     1681 cacgaattga aatacatcat ctccgaaggg gagcacaaac tgctggcaac gcgcctcaaa
     1741 gcctgtctca aacaggatta ttacgcttct ctcaacggcg gcgaatactt catcaggagc
     1801 ctgtatttcg acgacccgtt cgattccgcc gttgcggaaa aggcggacgg cgtcggctcg
     1861 cgcgataagt tccgcatccg catttacaac ctttcggaca gcacgatcaa gctggagcgc
     1921 aagcataagg aagggcagta catcaaaaag gacagcgtat ctctgacccg tgaggaatgc
     1981 gacgcgattg tccgcggaaa cctcgcctgc ctgctcgcca a
//
LOCUS       DAOMGY010000318         2021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_63588_length_2021_cov_3.212614, whole genome shotgun sequence.
ACCESSION   DAOMGY010000318 DAOMGY010000000
VERSION     DAOMGY010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63588_length_2021_cov_3.212614"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLR57_05505"
     CDS             <1..198
                     /locus_tag="PLR57_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40948.1"
                     /translation="EYQIRILDLRQKCLARKGFLQDLICEDGLHLTEEGQRFIGEELA
                     ALAQQAEQEDVALLRRTALHA"
     gene            complement(254..1039)
                     /locus_tag="PLR57_05510"
     CDS             complement(254..1039)
                     /locus_tag="PLR57_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HQQ40949.1"
                     /translation="MKEAMMNERMNKVRDYIQSRGEVSVSELQSLYEGYSSMTIWRDL
                     KQLEEQGCIRRVHGGVISVQSASFQVEGVYSKRARENTREKIAIAKAAVSLIRPGHSV
                     YLDAGSTLMAVANHMGSERYTIVTSGANIAIELSQRHTCDVLLTGGQISENTLSCSGA
                     QAEQFISSINIDLALMAPSGFSLQNGFTSGAQNESLLKRAVMKKAAKVVMLMDSSKIG
                     RSLPFTFATLADIDMLITEAPLPPEVAAEAKTCGVEVVVSQEQ"
     gene            1205..1924
                     /gene="nagB"
                     /locus_tag="PLR57_05515"
     CDS             1205..1924
                     /gene="nagB"
                     /locus_tag="PLR57_05515"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009334328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HQQ40950.1"
                     /translation="MKIHVCSGEQAAVLAADRYVELLKRKPNAVLGFATGSTPLQLYR
                     ELIRRYQWGELSFRQATSFNLDEYVGLDGSNPQSYRYFMNKNLFEQIDIPLERTFVPC
                     AKNCSEKSTVEYDEKIERAGGIDLQLLGVGRNGHIGFNEPGTSFDSLTHRVQLSRSTR
                     EANARFFHSLDEVPSEAVSMGIKTVMNARSILLFAQGEEKAEVIQKAVYGPVTEDVPA
                     SILQLHPFVELFLDPEAAKLL"
BASE COUNT          472 a          521 c          559 g          469 t
ORIGIN      
        1 gagtatcaga tacgaatcct cgatttacgg cagaagtgcc ttgccagaaa aggctttctg
       61 caggatctga tctgcgagga cggcctgcat cttaccgagg aaggccagcg gttcattggc
      121 gaagaacttg cagctcttgc ccaacaggcg gagcaggagg acgtggcgct gctgagaaga
      181 acggcgcttc acgcataagc aatagacgta agaatccccg gattgctccg gggattattt
      241 gttgctcgat ttgttactgt tcctgcgaaa cgacgacctc tacgccgcag gttttcgctt
      301 ccgcggcaac ctccggcggg agaggggctt ccgtaatcag catgtcgata tctgcaagcg
      361 tagcaaacgt aaacggaagc gaacgaccga tcttactgga gtccatgagc atgacgacct
      421 ttgcggcctt tttcatcacc gcgcgtttga gcaggctctc gttctgcgca ccgctggtaa
      481 acccgttctg cagcgaaaag ccggagggcg ccatgagcgc gaggtcgata ttgatagagg
      541 agatgaactg ttccgcttgt gcgccggagc aggagagagt attttcgctg atctgtccgc
      601 cggtaagcag tacgtcgcag gtatgccgct gggaaagctc tatggcaatg tttgcgccgc
      661 tggtaacgat tgtgtaccgc tcgctgccca tgtggttggc aacggccatg agcgtgctgc
      721 cggcgtcgag atacaccgaa tgtccggggc gaatcagcga aaccgcggct tttgcaatgg
      781 caatcttttc gcgtgtgttt tcgcgcgcgc gcttgctgta aacgccctct acctgaaacg
      841 aagcggactg aacggagatg accccgccgt gtacacgacg gatacagccc tgttcttcca
      901 attgttttaa atcgcgccag atggtcatgg aggagtagcc ttcatacagg ctttgcagct
      961 ccgaaacgct gacttcgccg cgcgattgaa tataatcccg tactttgttc atgcgctcgt
     1021 tcatcatggc ttccttcacc gccttcctta taacagccat tataacacgt atgttagaaa
     1081 acaacaaccc cagtgttaca gtcgctggtg ttattgaaat gtacggacaa ttggttgaca
     1141 aaagagttcc tttatagcat aatgtacttg tgaaatgtat ggtacatatt ggaggagaac
     1201 tttcgtgaaa attcatgtct gttccggaga acaagctgct gtacttgcgg ctgaccgcta
     1261 tgttgaactg ctcaaacgca aaccaaacgc tgtactgggc ttcgcaacag gttcgacgcc
     1321 gctgcaactc taccgcgagc tgatcaggcg gtatcaatgg ggagaactct cgtttcggca
     1381 ggcgacttcg ttcaatctgg acgaatacgt tgggctggac ggttcaaatc cgcagtccta
     1441 tcggtatttc atgaacaaga atctgttcga gcagatcgat attccactgg agcggacttt
     1501 cgtaccgtgt gcaaagaact gttccgaaaa aagcaccgtg gaatacgacg aaaagatcga
     1561 gcgcgcaggc ggcatcgatc ttcaacttct tggcgtgggt cgcaacggac atatcggctt
     1621 caacgaaccc ggcacgagct tcgattctct gacgcatcgc gttcagcttt cgcgctctac
     1681 gcgggaagca aacgcgcgtt tcttccattc cctcgacgaa gtaccttccg aggcggtcag
     1741 catgggcatc aagaccgtga tgaacgcgcg ctcgattttg ctctttgcgc aaggcgagga
     1801 gaaggcggaa gtcattcaaa aggcggtcta cggcccggtg acggaggatg ttccggcgtc
     1861 aattctgcaa ctgcatccgt tcgtcgagct atttctcgat cccgaagctg cgaagctact
     1921 ctaagaacga gagactgaaa aagtctgccg aaaggcagac tttttgaatt gaaaggggga
     1981 gacgcagggg ctctgcccct gaccccgcca aagggagttc t
//
LOCUS       DAOMGY010000319         2016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_63839_length_2016_cov_2.780724, whole genome shotgun sequence.
ACCESSION   DAOMGY010000319 DAOMGY010000000
VERSION     DAOMGY010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63839_length_2016_cov_2.780724"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLR57_05520"
     CDS             <1..744
                     /locus_tag="PLR57_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HQQ40951.1"
                     /translation="VDERVQAVRAAVSGANCGACGYAGCDAFAAAVVRGEASTSGCTP
                     GGSKSAKALAEIMGVSAASVEPMVARVRCQGNCNNVKPRFDYAGVPSCRAAGSLSGGP
                     NACEFGCVGLGDCVDRCPFGAIRMENGVAVVDQDVCTGCGVCVAVCPRGIIQMLPTDQ
                     TVVVLCRNKAIGRIAALQCKTACVGCKRCEKQCPTNSIVVKDGVAQIDETTCTRCGAC
                     IPVCPMHCIVNFYEREDEEVLSEQPKDEA"
     gene            complement(821..>2016)
                     /locus_tag="PLR57_05525"
     CDS             complement(821..>2016)
                     /locus_tag="PLR57_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQQ40952.1"
                     /translation="LRFGSDGTFTILIFSDLQDIQFVTRQIVAGEKSVLADTSADLIV
                     LLGDQMDGANPIMHIGNGDQNCLKTLKALMEPIADSGIPFAVVFGNHDYDAPISIADQ
                     VKFYESYETCVGVNYGTNLSDTGAYALPVYLSDGSAKAMELYFFDSGCYLPNGDYNTV
                     SAEQVAWYNEQSALLHTENGNQALPSLAFFHIPLPEVYDMLTEVEKGTPNSFEGVGTG
                     AGKYYLPDSDMIFTGDVNEPPCPSSSNNGLFDAFLNNGDVFLAVNGHDHTNSYIGSLH
                     GIDLANAPGSSYTSYGSEDTRGVRLFRFTEHNVKNYETVHVRYGEYNSPASFGYLRYF
                     FTTTIGLNGVPSMAKFVILFLVVLIAAVVILIIALKKRKKKRKLAAQATAVEPEPKET
                     SKSKN"
BASE COUNT          404 a          549 c          617 g          446 t
ORIGIN      
        1 gtggacgagc gcgtgcaggc tgtgcgcgcg gcggtctccg gcgcgaactg cggcgcctgc
       61 ggctatgccg ggtgcgacgc gtttgccgcg gcggttgtcc gcggggaagc gagcacgagc
      121 ggctgtacgc cgggcggctc gaaatccgca aaagcgcttg ccgagattat gggcgtttcc
      181 gcagcgtcgg ttgagccgat ggttgcgcgc gtgcgctgcc agggcaactg caataacgta
      241 aaaccgcgct tcgattacgc gggtgttcct tcctgccgcg cggcgggctc gctctccggc
      301 gggccgaacg cctgtgaatt cggctgtgtg ggtctgggcg actgcgtgga tcgatgcccg
      361 ttcggcgcaa tcagaatgga aaacggcgtt gcagtggtcg atcaggacgt gtgcaccggc
      421 tgcggcgtat gcgttgcggt ctgcccgcgc ggaatcatcc agatgctgcc caccgatcaa
      481 acggtcgtcg tgctctgccg caacaaggcg attgggcgta ttgcggcgct gcagtgcaaa
      541 accgcctgcg tcggctgcaa gcgctgtgaa aagcaatgcc cgaccaactc catcgtcgtc
      601 aaggacggcg ttgcgcaaat cgatgaaacc acctgcacgc gctgcggcgc ctgcattccg
      661 gtttgcccga tgcactgcat tgtcaacttt tacgaacgtg aggacgaaga agttctctct
      721 gaacagccca aagacgaagc gtaaaatacg gaataaaaca agagccggct gacgccggct
      781 cttgttttgt gtatcgttga cgctattttc agtggaaatc tcagttcttg ctcttgcttg
      841 tttctttcgg ctccggttcg actgctgttg cctgcgcggc cagcttgcgt ttctttttgc
      901 gcttcttcag tgctatgatc agaataacga ctgccgcaat cagcacaaca aggaacagaa
      961 tgacgaattt tgccatgctc ggcacgccgt tcagcccgat ggtcgtggta aagaagtacc
     1021 taaggtaccc aaagcttgcg ggcgagttgt attcgccata gcgcacgtgc accgtttcgt
     1081 agttctttac gttgtgttcg gtaaagcgaa acagccgcac gccgcgcgta tcctcgctgc
     1141 cgtaactcgt atacgaactg ccgggcgcat tggcaagatc gatgccgtgc agactgccga
     1201 tataggagtt ggtgtggtcg tgaccgttga cggcgaggaa tacgtcgcca ttgttcaaaa
     1261 acgcgtcaaa caggccgttg ttgctgctgg aagggcaggg aggctcgttc acatcgcccg
     1321 taaagatcat atcggagtcc ggcaggtagt atttgcccgc gccggttcca acgccttcaa
     1381 acgagttcgg cgtgcctttt tccacttcgg taagcatgtc gtagacttcc ggcaggggga
     1441 tgtggaaaaa cgcgagtgaa ggcagcgcct gattgccgtt ttcggtgtgc aagagcgcgc
     1501 tctgctcgtt ataccaggca acctgttcgg cggagaccgt gttgtaatcc ccgttgggca
     1561 gatagcatcc ggaatcgaag aagtacagct ccatcgcctt ggcggaacca tcgctcaaat
     1621 agaccggcag cgcatatgcg ccggtatccg aaaggttcgt tccgtagttg accccgacgc
     1681 aggtttcgta ggattcgtaa aacttgacct gatcggcaat ggagatcggc gcgtcgtaat
     1741 cgtggttgcc aaaaacgacc gcgaacggaa tgccgctatc ggcgatgggt tccatcagcg
     1801 ccttgagcgt ttttaagcag ttctgatcgc cgttgccgat gtgcataatc gggtttgcgc
     1861 cgtccatctg atcgcccagc agaacgatca agtcggcgga cgtatccgca agaacgctct
     1921 tttctccggc aacgatctgt cgggttacga actggatatc ctgcaggtcg gagaagatca
     1981 ggatggtaaa cgttccgtcg gatccgaacc ggaggt
//
LOCUS       DAOMGY010000320         2012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_64022_length_2012_cov_2.940215, whole genome shotgun sequence.
ACCESSION   DAOMGY010000320 DAOMGY010000000
VERSION     DAOMGY010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64022_length_2012_cov_2.940215"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLR57_05530"
     CDS             complement(<1..346)
                     /locus_tag="PLR57_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HQQ40953.1"
                     /translation="MKAKRHDMISKLIASENIETQEELAGKLRDHGFSVTQATVSRDI
                     KELRLIKVLTPEGRYRYATVEKAEADLQERFIRMFSNCVLSVTSAGSMIVIKTIAGSA
                     PAAAEAVDSLKWP"
     gene            complement(517..1308)
                     /locus_tag="PLR57_05535"
     CDS             complement(517..1308)
                     /locus_tag="PLR57_05535"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HQQ40954.1"
                     /translation="MIVGFAGNLEKPGIAEIRSKLMAQAERLGCECVAFDSVEAVRLT
                     RLAPELLVVIGGDGSLLRFASVAAGRNLPILGVNLGRIGFLSEIAADEFEQSLERIQK
                     GDYRLERRMMLRCRVNDGECWHCLNDILVFKQSFSGTIQVGLSCDGQEVGTVFCDGII
                     ASTPTGSTAYNLSAGGPVVTQNLDAIVVTPVCSHTLHVRPVVSSACSIWSFQVNETGF
                     VAGDGIRLSEVRPDDRIEVTKSDRIVQFIRFSEQNVFELIQRKLS"
     gene            complement(1393..>2012)
                     /locus_tag="PLR57_05540"
     CDS             complement(1393..>2012)
                     /locus_tag="PLR57_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HQQ40955.1"
                     /translation="KLQKAMEEFPISLEGTVAADIGASTGEFTDCMLQHGAKKVYAID
                     VGYGQLDWSLRSDERVVVLERTNARFMEPKWFAEPLDFASIDVSFISLDKILPPLFTC
                     LRDGGEVVALVKPQFEAGRNKVGKNGVVTDEETHLEVVERMTGVALSAGFCVKGLSYS
                     PIKGPKGNIEFLLYLQKCIDVTIPTSKVDHNEAFSVVRQAHNICI"
BASE COUNT          462 a          559 c          524 g          467 t
ORIGIN      
        1 ccggccactt gagcgaatcg accgcttccg ccgccgccgg cgcgctgccc gcgatggtct
       61 taatgacgat catgctgccc gcgctggtga ccgagaggac gcagttgctg aacatgcgga
      121 taaaccgctc ctgcaggtcg gcttcggcct tttccaccgt cgcatagcgg taccggcctt
      181 ccggcgtgag caccttgatc agccggagtt ccttgatgtc gcgcgagacg gtcgcctgcg
      241 ttacggaaaa tccgtgatcg cgcagtttcc ccgcaagttc ttcctgcgtt tcgatgttct
      301 ccgaagcgat cagcttggaa atcatgtcat gacgttttgc tttcacgata ctatccccaa
      361 tacataataa taatgagtgt gcgtgttctg atatagctga gcgcaggttt ccgccggtcg
      421 ggatggagcg ccgcgctcaa aggagcggat tgagctgaca gtttttttta aaaaacggtt
      481 cgaataaata cggatgtccg gattgatgcg gaaagctcag gacagttttc gttgaatgag
      541 ttcaaaaacg ttttgttccg aaaaacggat aaactgtacg attcggtcgg acttggtcac
      601 ttcgattctg tcgtccgggc gaacctcgct gaggcggatg ccgtcgccgg cgacaaatcc
      661 cgtttcgttg acctgaaacg accagatcga gcaggccgag gatacaacgg ggcgaacgtg
      721 tagagtgtgc gagcaaacgg gagtaacgac gattgcgtcc aggttttgcg tcaccacggg
      781 tccgcccgcg gagaggttgt aagccgtgga gccggtcggc gttgaagcga tgatgccgtc
      841 gcaaaaaacg gttccaactt cctgcccgtc gcagctcagg cctacctgaa tcgtgccgga
      901 gaacgattgc ttaaacacca gaatgtcgtt gagacagtgc cagcattcgc cgtcgttaac
      961 gcggcagcgg agcatcatgc gccgctccag acggtaatcg cctttctgaa tccgctctaa
     1021 gctctgctcg aattcatccg cggcgatttc gcttagaaat ccgattctgc caaggttcac
     1081 accaagaatc ggcagattcc gtcccgcggc gacggaagca aatcgcaaaa gcgacccgtc
     1141 cccgccgatg acgaccagca actccggcgc aagccgagtg agccgcaccg cttccacgct
     1201 gtcgaacgcg acgcattcgc agcccagacg ctcggcctga gccatcagct tggatcggat
     1261 ttcggcaatg ccaggttttt caaggtttcc ggcaaagccg acaatcatgg aaaatacccc
     1321 aaattaaata atatatcggc attatacagc gcaatacata aaaatacaag agtaattatg
     1381 aatatgtatt ctttatatac agatgttgtg tgcttgccgc acaacggaaa aggcttcgtt
     1441 atggtctact tttgatgtcg gaattgttac atctatacat ttttgcagat agagtaaaaa
     1501 ttcaatattc ccttttggcc ccttaatggg tgaatacgag agacctttta cgcaaaatcc
     1561 cgcagataag gcaacgccgg tcatccgctc tacgacttcc agatgcgttt cctcgtccgt
     1621 taccacgccg tttttgccta ctttgttccg tcccgcttca aactgcggct tgacgagcgc
     1681 caccacttcc ccgccgtctc tgaggcaggt aaacagcggc ggcaggattt tatcgagcga
     1741 gataaacgaa acgtcgatgg atgcaaagtc gagcggttcg gcaaaccact ttggttccat
     1801 aaaccgcgcg ttggtgcgtt ccagaaccac aacgcgttcg tcgcttctca gcgaccagtc
     1861 gagctggccg tagccaacgt cgatggcgta cacttttttt gcgccgtgct gcagcatgca
     1921 gtccgtaaac tcgcccgtag atgcgccgat atccgccgca accgtgccct ccagcgaaat
     1981 gggaaattct tccatcgctt tctgcagctt at
//
LOCUS       DAOMGY010000321         2003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_64495_length_2003_cov_2.622177, whole genome shotgun sequence.
ACCESSION   DAOMGY010000321 DAOMGY010000000
VERSION     DAOMGY010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64495_length_2003_cov_2.622177"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLR57_05545"
     CDS             complement(<1..756)
                     /locus_tag="PLR57_05545"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ40956.1"
                     /translation="MNIDERHVCARCLSPITEGARCELCGFLESEYAPEPHHLPLETL
                     LQRRYLVASVLGEGGFGVTYAAWDLTLDRPVAVKEYFPRFTAERDASKTYAVNPLSGG
                     SNKNWFAAGRDRFLREARILATLSGIRGVVPALDCFEENGTAYIVMDFIRGVSLRRLA
                     DQFGGKLPASELLPMLRPVLDALILVHRSGLLHRDISPDNILIDRDGSAWLIDFGAAE
                     EAQRGLDDVSRTILLRKGYTPLEQYDSHGEQGVW"
     gene            complement(818..1243)
                     /locus_tag="PLR57_05550"
     CDS             complement(818..1243)
                     /locus_tag="PLR57_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQQ40957.1"
                     /translation="MEKSDKKTDYTEYAIAWMQAHEEPLPPDKQVALESIPSYLNKKL
                     GEQNLSTAQLFEAAGTERSTGYKIMNGQLLPSRDSLLRVAFVLKLDVNETQYLLKVGR
                     RAKLTPRDSRDAAILHCIIRHMSLMDANLTLDEAGEEPL"
     gene            complement(1333..>2003)
                     /locus_tag="PLR57_05555"
     CDS             complement(1333..>2003)
                     /locus_tag="PLR57_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HQQ40958.1"
                     /translation="IMTVQPKLVTHWISDDPEAATSVLCPINIEFLSNGTCLQDGQNA
                     DYRILGDRVRINGCTFDYSCSRDQLTLVGESGSATYTSCGSSERYEYTIKDGEAEITQ
                     NDDNTSWIAVADEFEGCPVTRIGDYAFASENSIVQIDLPDTLTEIGDYAFFFCPHLEE
                     IILPESLKTIGENAFSYCTSLKRVVLPYGIEQIGDNAFSGCTALEEVVYPDGTACTIA
                     DFAH"
BASE COUNT          421 a          530 c          587 g          465 t
ORIGIN      
        1 ccagacgccc tgctcgccat gggaatcgta ctgttccagt ggcgtatagc cttttcggag
       61 cagaatcgtc cggcttacgt cgtcgaggcc gcgctgggct tcctccgccg cgccgaaatc
      121 gatgagccag gcggagccat ccctgtcgat gaggatgttg tccgggctga tatcccggtg
      181 cagcaagccg gaacgatgca ccagaatcag cgcgtccagc acggggcgga gcatgggcag
      241 aagctcggaa gcgggcagtt ttccgccgaa ctgatcggca agccttcgaa ggctgacacc
      301 gcgaatgaag tccataacga tatacgccgt gccgttttcc tcaaagcagt ccagcgcggg
      361 cacgacgccg cggatgccgg agagcgttgc cagaatgcgc gcttcgcgca aaaaccggtc
      421 gcgcccggcg gcaaaccagt ttttgttcga gccgccagaa agcgggttga cggcgtaggt
      481 cttactggcg tcgcgttcgg cggtaaagcg cggaaaatac tcctttaccg ccaccgggcg
      541 gtcgagggtt aagtcccagg cggcgtaggt cacgccaaaa ccgccttcgc ccagcacgct
      601 ggcgacaagg tatcgccttt gcagcagggt ttccaacggg aggtggtgcg gctcgggcgc
      661 gtattcgctc tccaaaaagc cgcagagttc gcagcgcgct ccttccgtaa tcgggctcag
      721 gcagcgcgcg cagacatggc gttcgtcgat gttcatgcgg ttcctttcct tctgcggcgg
      781 tcgtcgatag ccgccgcgcg gacagaaaaa ggttcggtta cagcggttct tctcccgctt
      841 cgtcgagggt gaggtttgcg tccatcagcg acatatgcct gatgatgcag tgcagaatcg
      901 ccgcgtccct agaatcccgc ggggtgagct ttgctctgcg gccaaccttg agcaggtact
      961 gggtctcgtt gacgtccagc ttgagcacaa aggcaacgcg cagcagagaa tcgcggctgg
     1021 gcaagagctg cccgttcata attttatagc ctgtggaacg ctcggttccg gcggcttcaa
     1081 atagctgggc ggtagacaag ttctgctcgc cgagcttttt gttgaggtag gaaggaatcg
     1141 actccagcgc aacctgcttg tcgggcggga gcggttcttc gtgcgcctgc atccaggcga
     1201 tggcgtattc ggtgtagtcg gtctttttgt cgctcttttc catgctggga aactcctttt
     1261 attttgcgca gtacgcgttt ttagtaaatg taataggggt caatttgggt ggcattatgc
     1321 ggcgtagatg aatcaatgcg cgaaatctgc aatcgtacag gctgttccat cggggtacac
     1381 gacttcttcc agcgcagtac aaccggagaa cgcgttgtca ccgatttgct caatgccata
     1441 aggcaatacg acgcgcttga gtgaggtgca atagctgaac gcgttttcac cgatcgtttt
     1501 caaactttcg ggaagaataa tctcctccag atgcgggcag aagaagaatg cgtaatcacc
     1561 aatctccgtc agcgtgtctg gcaggtcaat ctgtacgatc gagttttcgg aggcgaatgc
     1621 ataatcgccg attcgggtta cagggcagcc ttcaaactca tctgcaactg caatccagga
     1681 ggtgttgtcg tcgttttgag tgatttcggc ttctccatct ttgattgtgt attcgtaccg
     1741 ctcacttgaa ccacaggagg tatatgttgc ggatccggat tcgccaacga gagttaactg
     1801 atcgcgactg caggagtagt caaatgtaca tccgtttata cggacacggt ctcccaaaat
     1861 ccggtaatca gcgttttgcc cgtcctgaag gcatgttccg tttgaaagaa actcgatatt
     1921 aataggacat agaacgcttg ttgctgcctc aggatcatct gaaatccaat gtgtgacaag
     1981 ctttggttga acggtcataa tga
//
LOCUS       DAOMGY010000322         1999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_64687_length_1999_cov_3.283951, whole genome shotgun sequence.
ACCESSION   DAOMGY010000322 DAOMGY010000000
VERSION     DAOMGY010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64687_length_1999_cov_3.283951"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLR57_05560"
     CDS             <1..525
                     /locus_tag="PLR57_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HQQ40959.1"
                     /translation="AGADVAATSRALSLGAKQAGEFDLILCGKQTTDGDTAQVGPELA
                     ERLGMEHAANVVCIVGMEDRGITVTIDLDNVLQTQWMAFPCLLTIDKDANTPRLPSYR
                     RRQAVDAKKQISIYAASELPGAEETMLGLKGSPTQVERIFPPENNVEKQLFTGTDEEL
                     ADALYDMLRERKFV"
     gene            535..1731
                     /locus_tag="PLR57_05565"
     CDS             535..1731
                     /locus_tag="PLR57_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HQQ40960.1"
                     /translation="MGLIIHQDKITAEVAAQMQSICPFGALQYTSGTLTVNAACRSCK
                     LCVKKGPHGAATWADEAQKELDKSAWRGVAVFAEMRGGALHPVTLELLGKARELAAVT
                     GEPVYAVLIGGGASGLAPELLHVGADKAYVYDDPVFERFMVSPFAAAFYDFIQKVKPS
                     SILVGATSLGRSLAPKVAARCDAGLTADCTVLEMKQNTDLVQIRPAFGGNIMAQIVTP
                     NTRPQFCTARYKIFSTRERSETTKVEIVNMPVTDAMRQSKTTVITIKEKPKETDISEA
                     DAIVACGRGFTKEADLAMAKELAELLHAQMAGTRPMIEAGWLDPKRQIGLSGRTVKPK
                     LLIALGISGSVQFAAGMKGSERIVAVNKDPNASVFEFAHVGLVGDLYEVVPRLIERIR
                     EGARNV"
     gene            1724..>1999
                     /locus_tag="PLR57_05570"
     CDS             1724..>1999
                     /locus_tag="PLR57_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015957783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQQ40961.1"
                     /translation="MYKKMIGEDIAFLKKATSPERVLTGGQIGEDYSHDELGGVSSMP
                     EALVKVLSTEEVSAIMKYANEQSIPVVVRGSGTGLVGAGVALYGGIML"
BASE COUNT          441 a          522 c          690 g          346 t
ORIGIN      
        1 gcgggcgcgg acgtcgctgc tacaagccgc gcgctttcgc ttggcgcgaa gcaggcgggc
       61 gaattcgatc tgatcctttg cggaaagcag acgacggacg gggacaccgc gcaggtcggg
      121 ccggagcttg cggaacggct gggcatggag cacgcggcga atgtagtgtg catcgttgga
      181 atggaggatc gcgggattac ggtaaccatc gatctcgaca acgtgctgca gacgcagtgg
      241 atggcgtttc cttgcctgct gaccatcgat aaggacgcga acaccccgcg gctgccgtcc
      301 taccggcgcc ggcaagcggt cgatgcaaaa aagcagattt ccatatacgc ggcaagcgaa
      361 ttgcccggcg cggaggaaac catgctcggg ttgaaaggtt cgcccacgca ggtcgagcgg
      421 atatttccgc cggaaaacaa cgtggaaaag cagctgttta ccggcacaga cgaggaactt
      481 gcggacgcgt tgtacgacat gctgcgcgag cgtaagtttg tttgaggagc atagatggga
      541 cttatcatcc atcaggataa aataacggcg gaagtcgcgg cgcagatgca gagcatttgc
      601 ccgtttggcg cgctgcaata tacaagcggg acgctaacgg tcaatgcggc gtgcagaagc
      661 tgcaaactct gtgtgaaaaa gggaccgcat ggcgcggcta catgggcgga cgaggcacaa
      721 aaagagctgg acaaatccgc ttggcgcggc gttgcggtgt ttgccgaaat gcgcggtggc
      781 gcgctccatc cggttacgct ggagttgctt ggcaaagcgc gcgaactcgc ggcggtgacg
      841 ggagaacccg tgtacgccgt gctgattggc ggcggcgcaa gcggtctggc accggaactt
      901 ttgcacgttg gcgcggacaa ggcgtatgtc tacgacgatc cggtgttcga gcggttcatg
      961 gtttcgccat ttgccgcggc gttctacgat ttcatccaaa aggtcaagcc gtcgtcgatc
     1021 ctcgtgggtg cgacgagtct tggccgttcg cttgcgccaa aggtcgccgc gcggtgcgac
     1081 gcgggactca cggcggactg tacggtgctc gaaatgaagc agaataccga ccttgtgcag
     1141 atacgccccg cctttggcgg gaacatcatg gcgcagattg tgacgccgaa tacgcgcccg
     1201 cagttctgca cggcgcggta taagatcttt tctacgcgcg agcgaagcga aacgacaaag
     1261 gtcgaaatcg taaacatgcc ggttacggac gccatgcggc aaagcaaaac gacggtgatt
     1321 acgatcaaag agaagccgaa ggagaccgat atttcggaag cggacgcgat cgtcgcctgc
     1381 ggacggggtt tcaccaagga agcggacttg gcgatggcaa aggagctcgc agaattactg
     1441 cacgcgcaga tggcgggcac gcggcccatg atcgaggcgg gttggctcga cccgaagcga
     1501 cagatcgggc tgtccgggcg gacggtaaaa ccgaaactgc tgatcgcact cggaatctcc
     1561 ggttcggtgc agttcgcggc ggggatgaag ggcagcgaac ggatcgtggc ggtcaacaag
     1621 gacccgaacg cgagcgtctt cgagttcgcg cacgtcgggc tcgtcggcga cctgtacgag
     1681 gtcgtgccgc ggctgatcga acgaatccgg gagggcgcgc ggaatgtata aaaagatgat
     1741 aggcgaggat atcgcgtttc tgaaaaaagc aacgtctcca gagcgcgtgc tgaccggcgg
     1801 acagattggc gaggactaca gccacgacga gctgggcggc gtgtcgagca tgccggaagc
     1861 gctcgtgaaa gttttgagca cggaggaagt ctccgcgatc atgaaatatg ccaacgagca
     1921 gagcatcccc gtggtcgtgc gcggcagcgg gacaggattg gtcggcgcgg gggtcgcgct
     1981 gtacggcggg atcatgctg
//
LOCUS       DAOMGY010000323         1997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_64785_length_1997_cov_4.001030, whole genome shotgun sequence.
ACCESSION   DAOMGY010000323 DAOMGY010000000
VERSION     DAOMGY010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64785_length_1997_cov_4.001030"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLR57_05575"
     CDS             <1..825
                     /locus_tag="PLR57_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ40962.1"
                     /translation="VGITFVYVTHDQEEALTMNDTIVVLKDGVIQQIGTPTDVYNEPK
                     NPFVADFIGESNILPGIMVQDYKVRMNGVVYECVDKGFGKNTEVDVVVRPEDIDIIPA
                     DKARVSGKVVSVVFMGVHYEVDVDCGGYEWVIQTTDYVERGETIGISIPPDAIHVMKK
                     TQQTNLFDALAYPDDEEVEFCGHRFACKGLEGFETKDDVLVAIVPGDVRLIAPQDSDV
                     TGFVKVCMFLGTHYEVTVTCDEEDWIAYSNEYMEPGEEIGLAVDADKIAVSANGAE"
     gene            914..1693
                     /locus_tag="PLR57_05580"
     CDS             914..1693
                     /locus_tag="PLR57_05580"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40963.1"
                     /translation="MKRSLFATPYIVWMAVFILSPMLLILYYAFTSDGAVSLETIISA
                     ASWDNLRVLLDSVRFALYTTVLCLLLGYPVAYLLSRMNKSVAALLSVFFVVPMWMNFL
                     LRTYAWKVLLGAFTKILYTEAAVLLGMVYNFLPFMILPIYTTLIKLDKSYIEAASDLG
                     ANPATTFTKVVLPLSMPGVISGITMVFIPSTTTFAISQLLGGGKTMLYGDLIYMKFIT
                     EQSWNAGSALSVILLVFVLISMTFMKKAERGAAEEGGGRLW"
     gene            1687..>1997
                     /locus_tag="PLR57_05585"
     CDS             1687..>1997
                     /locus_tag="PLR57_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQQ40964.1"
                     /translation="MVNRLKKFFAGTYLGLMLVFLYAPILILIVFSFNQSKTLGNWTG
                     FTFNWYAELFRTPEITDALVVTLSVAAISSIVATVLGAYAAIGLHAMKRKYRLVYENI
                     S"
BASE COUNT          416 a          553 c          582 g          446 t
ORIGIN      
        1 gtggggatta cctttgtgta cgtcacccac gatcaggaag aagcgctgac catgaacgat
       61 accatcgtcg tgctcaagga cggtgtgatt cagcagatcg gtaccccgac ggacgtctac
      121 aacgagccga agaacccgtt tgtggcggac ttcataggcg aaagcaacat cctccccggc
      181 atcatggtac aggactacaa agtccgcatg aacggcgtgg tttacgagtg cgtggacaag
      241 gggttcggca agaataccga ggtggacgtc gtcgtccgcc cggaggatat cgacatcatc
      301 cccgcggaca aggcgcgcgt gagcggcaag gtcgtctcgg tcgtgtttat gggcgtgcat
      361 tacgaagtgg acgtggactg cggcggctat gagtgggtca tacagaccac ggattacgtc
      421 gagcgcggcg aaaccatcgg catttccatt ccgccggacg ccatacatgt gatgaaaaag
      481 acccagcaga ccaacctctt cgacgcgctg gcttatccgg acgacgagga agtcgaattc
      541 tgcggccacc gctttgcctg caagggtctc gagggattcg aaaccaagga cgacgtgctc
      601 gttgccatcg tgccgggaga cgttcgtctg atcgcaccgc aggacagcga cgtgacgggc
      661 tttgtaaaag tctgcatgtt ccttggtacg cactacgaag tcaccgtcac ctgcgacgag
      721 gaggattgga ttgcctactc gaacgaatac atggagcccg gcgaggaaat cggcctcgcg
      781 gtggacgcgg acaagattgc cgtcagcgcg aacggcgcgg aataacgaaa cgcgtgcgtt
      841 tcacgcttga ggacacgaaa tgaaaattct gcgcaagaaa agaaatagag aatccggcgc
      901 gcgcggcttt cagatgaagc ggagcctgtt tgccacgccg tatatcgtct ggatggcggt
      961 gttcatcctc tcccccatgc tgctcatcct ctactatgcg ttcacgagcg acggtgcggt
     1021 ttccctggaa accattattt ccgccgcaag ctgggataat ctccgcgttc tgctggactc
     1081 cgtccgcttt gcgctgtata ccacggtgct ctgtctgctg cttggctatc cggttgcgta
     1141 cctgctttcg cggatgaaca agtccgtcgc ggcgctgctc tccgtgttct tcgttgttcc
     1201 gatgtggatg aacttcctcc tgcgtaccta tgcctggaag gtgctgctcg gcgcgtttac
     1261 caagattctc tatacggaag cggcggttct gctgggcatg gtgtataact ttttgccgtt
     1321 tatgatttta ccgatctata ccacgctcat taagctcgac aagagctaca tcgaggccgc
     1381 gagcgacctt ggcgcgaacc cggcgacgac gtttaccaag gtggtgctcc cgctctcaat
     1441 gccgggcgtc atctccggca tcacgatggt gttcattccg tccacgacga cgttcgccat
     1501 ctcccagttg ctcggcggcg gaaaaacgat gctctacggc gacctgatct acatgaagtt
     1561 tatcaccgaa cagtcctgga acgcgggcag cgcgctttcg gtcatcctgc ttgtgttcgt
     1621 tttgatctcg atgacgttta tgaaaaaggc cgagcgcgga gcggcggaag aaggaggcgg
     1681 caggctatgg taaaccgtct caaaaagttc ttcgcgggca catatctcgg cctgatgctg
     1741 gtgtttctgt acgcgccgat actgattctg atcgtctttt cgttcaatca gagcaagacg
     1801 ctcggcaact ggacgggctt tacgttcaac tggtacgcgg agctcttccg cacaccggag
     1861 attacggacg cgctggtggt aacgctttcg gtcgcggcca tttcgtctat cgttgcaacc
     1921 gtgctcggag cgtacgcggc tatcggcctg cacgccatga agcgcaagta ccggctggtg
     1981 tacgaaaaca tctcgca
//
LOCUS       DAOMGY010000324         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_64840_length_1996_cov_3.204534, whole genome shotgun sequence.
ACCESSION   DAOMGY010000324 DAOMGY010000000
VERSION     DAOMGY010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64840_length_1996_cov_3.204534"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1107
                     /locus_tag="PLR57_05590"
     CDS             160..1107
                     /locus_tag="PLR57_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40965.1"
                     /translation="MKTPKNAIVAGISLLFAVLLGGCGVLSAPANGQSTATPAADSVS
                     IAASSPTPAEPTPKPEIVVFGAEASRSFQEGVTDAALQGQYAITFVSGGIETLASYQP
                     SGSVCAVVYWNDPKDALPAVSFPVFAYAARGQTGAGNVPLLVYDASGAAEDALERAIA
                     YPPHETPVRLIGLFSSETSPGYTAWKAAAAKGRIFPKAEFFLDDLEMQAASVWFAERL
                     DEFYPGMIDAVYAETGDLAIAAVHQLRGRTREDMEVFSASSDANADLSLSSLMPAVAG
                     ADLYKAGQLCYEGAAALLQGKIAESSVLSPSILEYTPNP"
     gene            complement(1171..>1996)
                     /locus_tag="PLR57_05595"
     CDS             complement(1171..>1996)
                     /locus_tag="PLR57_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQQ40966.1"
                     /translation="DADPEDEATPTPRGIWKIGGRERFGKFANFGGEYARYWVQIVGS
                     IYFHSLLFGDRTVDSLKRSPFYDLGEKVSHGCVRLYVEDAKWLYYYACPGTTVEISNS
                     ERSNRALHKALETKLSFAEYNAFQKTITDDAPELPNRSAWVMVEGARLRKGSGASFDT
                     VCRLNVGDELEVLLESEVWVKARFGKYEGYVLRGYISYTEGVMETSETATLLKTTEWL
                     YEEPSKDSTQICKVPSRVSVKVLEQTEDGWTKLEYQNEVGYIPTSRLTTGWGLIMD"
BASE COUNT          374 a          609 c          559 g          454 t
ORIGIN      
        1 tctgccgcaa cgcccaaccc gtaataaaac ttgcttcaca acgaaccgcg cagacttgcg
       61 cggttcgttt tcttttgcgc ttctcctgcc tgtcaacttt gtgtgatttt cggtttctcc
      121 ctcttggatg gttttacagc cacgtggtat actggctcta tgaaaacgcc gaaaaacgca
      181 atcgtcgccg gtatctcact gctgtttgcc gtcctgttag gcggctgcgg cgttctttcc
      241 gctccggcaa acggacaatc caccgcaacg cccgcggcgg actccgtttc cattgcagcg
      301 tcctctccaa cccccgcgga accgacgccg aagccagaaa tcgtggtgtt tggcgcggag
      361 gcctcccgct cgtttcagga aggggtaacc gacgcggctt tgcaggggca atacgccatt
      421 acgtttgttt ccggcggaat agaaacgctc gcttcgtacc agccaagcgg ttccgtctgt
      481 gcggtcgttt attggaatga cccgaaggat gcgctgcctg cggttagttt tcctgtgttc
      541 gcctatgccg cgcgcgggca gaccggcgcg ggaaacgttc cgctcctcgt ttacgacgcg
      601 tccggcgcgg cggaggacgc tcttgagcgc gcaatcgcct atccgccgca cgaaacgccc
      661 gtccggctca tcggcctgtt ctccagcgaa accagccccg gctatactgc ttggaaagcg
      721 gcagctgcaa aagggcgaat atttcccaaa gcggagttct ttctggacga tttggagatg
      781 caggcggcga gcgtctggtt tgccgaacgg ctggacgagt tctaccccgg catgatcgac
      841 gcggtctatg ccgaaaccgg cgatctggcg attgcggctg tccaccagct gcgcggacgc
      901 accagagagg acatggaggt attctccgct tcttcggacg cgaacgccga tttatcgctc
      961 tccagcctga tgcctgccgt cgccggggcc gacttataca aggcaggcca actttgttat
     1021 gaaggtgctg ccgccctcct gcagggcaag atagcagaat cttccgtgct ctccccctcc
     1081 atactggagt acacaccaaa cccttaaaac gcaggcaaac aaaaaggcgc agatgcgcct
     1141 ttttatttgt gatcggataa gccctgccaa tcaatccatt attagacccc agccggtggt
     1201 caggcggctg gtggggatgt agcccacttc gttttggtat tccagcttcg tccagccgtc
     1261 ctccgtctgt tccagcacct taacggaaac gcgcgagggg accttgcaga tctgcgtaga
     1321 atccttgctt ggctcttcgt agagccattc ggtcgttttg agcagcgtcg cggtttcgct
     1381 ggtttccatg accccctcgg tatagctgat gtagccgcgc agaacgtatc cttcgtattt
     1441 gccaaaacgc gccttgaccc aaacctcgct ttcgagaagc acttccaact cgtcgccaac
     1501 gttcaggcgg cagaccgtgt cgaaggatgc gccgctgcct ttgcgcaggc gcgcgccctc
     1561 caccatgacc catgcgctgc ggttaggcag ttccggcgcg tcgtcggtaa tcgtcttttg
     1621 aaacgcgtta tattcggcaa acgaaagctt tgtttccagc gctttgtgca gggcgcggtt
     1681 gctgcgttcg gagttgctga tttcaacggt cgtgcccggg caggcatagt agtagagcca
     1741 ctttgcgtcc tccacataca ggcgcacgca cccgtgggat accttttcgc caagatcgta
     1801 aaacggtgac cgcttgaggg aatcgactgt tcgatcgcca aagagcagcg agtggaaata
     1861 gatgctgccg acaatctgca cccagtagcg cgcatactcc ccgccgaagt tggcgaactt
     1921 gccgaagcgt tcgcgcccgc cgatcttcca gatgccgcgc ggcgttggag tagcttcgtc
     1981 ctcggggtcg gcgtcg
//
LOCUS       DAOMGY010000325         1990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_65149_length_1990_cov_2.925581, whole genome shotgun sequence.
ACCESSION   DAOMGY010000325 DAOMGY010000000
VERSION     DAOMGY010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65149_length_1990_cov_2.925581"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /gene="alr"
                     /locus_tag="PLR57_05600"
     CDS             <1..899
                     /gene="alr"
                     /locus_tag="PLR57_05600"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQQ40967.1"
                     /translation="AGFKAPVLLLGATDEEHMNIVIERDLIPTVFSVETLSGLQNHAK
                     ALNKTCHIHIKVDTGMNRLGFSSENEFVKALELLKECPNVVFDGLFTHFAVSELPDPS
                     FTLGQAERFRKYAELAHEHGFFPILHASNSAAAIHLPETQFDMVRGGIAMYGCHPAGH
                     AVAGVDLRPVMRWHTAITHIKTIGPNEGVSYGLKFVSPCEMRIGTLAVGYGDGYKRCL
                     SGKADVLVRGKRARQIGTICMDQMMIDLSNVPDAEVDDDVILIGSQGSEEITADELAE
                     KAGTISYEILLSVSDRVPRFYE"
     gene            complement(958..>1990)
                     /locus_tag="PLR57_05605"
     CDS             complement(958..>1990)
                     /locus_tag="PLR57_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQQ40968.1"
                     /translation="NLLFPGRGLCGDVIVHPLETDPQILYSAKKLEIAFHAARFDDAA
                     RELDVFLADPDATFGVVLFEQSDAAKLLPKRPLDSHKGKNGRALLCVGSGQYTGAALL
                     SAGAALRAGAGLLTAAVPGAVKPAFAQLPEAMAVSVSEGSNWDEAACERAFELLEGKN
                     AVGVGCGVGEGNILPLIKHALESGVPLLLDADALNQMANKKEMLSLLHSNVVLTPHPA
                     EMARLTGEPLEAILSDPIEAARTYAKRWNCVVLLKGATTCVSDGTQVRLNVSGNPGLA
                     KGGSGDVLSGIITALLAQGLNAFDAASVGAFLLGASADVALELLRERALIARDVIEVV
                     ERTVNATFL"
BASE COUNT          410 a          610 c          574 g          396 t
ORIGIN      
        1 acgcgggatt caaagcgcct gtgcttctgc ttggcgctac ggacgaagag cacatgaata
       61 tcgtcatcga gcgggacctg attcccacgg tcttttccgt tgaaacgctt agcggtttgc
      121 agaaccatgc caaagcgctc aataaaacct gccatatcca catcaaggtg gataccggca
      181 tgaaccgcct cggcttttcg agcgaaaatg aattcgtaaa agcgttggag ctcttgaaag
      241 aatgcccgaa cgtcgtgttc gacgggctgt ttacgcactt tgcggtctcc gaactgcccg
      301 acccttcgtt tacgcttggg caggcggagc gtttccggaa gtatgccgaa ctcgcgcacg
      361 aacacggatt tttcccaatt ctgcacgcaa gcaacagcgc ggcagcgatt cacctgccgg
      421 aaacgcagtt cgatatggtg cgcggcggta ttgccatgta cggctgtcac cccgccggac
      481 atgcggtagc gggcgtcgac ctgagacccg tgatgcgctg gcacaccgcg attacgcata
      541 ttaagaccat cgggccgaac gaaggggtca gctacgggct caagtttgta tccccctgcg
      601 aaatgcggat cggcaccctc gccgtaggtt atggcgacgg ctacaagcgg tgcctttccg
      661 gcaaggcgga cgtgctggta cgcggcaaac gcgcgcgtca gatcggcacc atctgtatgg
      721 atcagatgat gatcgatttg agcaacgttc ccgacgcgga ggtggacgac gacgtaatct
      781 tgatcggctc acagggcagt gaggagatta cggcggacga gctggccgag aaggcgggca
      841 cgatcagcta cgaaatattg ctcagcgtct ccgaccgcgt cccccgattc tacgaatagc
      901 atcaaaaagc cacctccggg cggctttttt agtattaggt tcttttgcta ctttctttta
      961 aagaaaagta gcgtttactg tcctttcaac aacctcaatc acgtcccgcg cgatcagcgc
     1021 gcgctcacgg agcagctcca gcgcgacatc cgccgacgcg ccgagcaaga acgccccgac
     1081 gcttgccgca tcgaacgcgt ttaatccctg cgcgagcaga gccgtaatga tgcccgatag
     1141 cacgtcaccg ctgccgccct tggcaagccc tggattgccg gaaacgttca ggcgcacctg
     1201 tgttccgtcc gaaacgcagg tcgtcgcacc cttgagcagc acaacgcagt tccagcgttt
     1261 ggcatacgtg cgcgcggcct cgatgggatc gctgagaatc gcttccagcg gttctccagt
     1321 gagccgcgcc atctccgccg gatgcggggt caggacgacg tttgaatgca ggagagacag
     1381 catctctttt ttattcgcca tctggttgag cgcgtccgcg tccagcagga gcggtacgcc
     1441 gctttccagc gcgtgtttga tcagcggcag gatgttccct tcccccacgc cgcagccgac
     1501 gccgaccgcg tttttacctt ccagcagttc aaaagcgcgc tcgcaggctg cttcgtccca
     1561 gtttgaacct tccgaaacgg aaacggccat cgcttccggc agctgcgcaa aagccggctt
     1621 gaccgcgccc ggcacggcgg cggtcaacag ccccgcgccc gcacggagcg ccgcgcccgc
     1681 ggagagcaga gccgctcccg tatattgccc gctgccaacg cagaggagtg cgcgaccgtt
     1741 tttgcccttg tgggaatcca gcgggcgttt gggcagcagt tttgccgcgt cggactgctc
     1801 gaataaaaca acgccgaacg ttgcgtcggg atcggctaaa aacacatcca gctcccgcgc
     1861 cgcatcgtca aaccgcgcgg catgaaacgc aatttcgagc ttttttgcgc tgtagagaat
     1921 ttgcggatct gtttccagcg ggtggacgat cacgtccccg cagagcccgc ggcctggaaa
     1981 gagcaggttc
//
LOCUS       DAOMGY010000326         1989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_65199_length_1989_cov_2.440021, whole genome shotgun sequence.
ACCESSION   DAOMGY010000326 DAOMGY010000000
VERSION     DAOMGY010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65199_length_1989_cov_2.440021"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="PLR57_05610"
     CDS             complement(<1..106)
                     /locus_tag="PLR57_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ40969.1"
                     /translation="MRQDIELRLETPQDYSEVEHITREAFWNKHVPGCD"
     gene            complement(211..1032)
                     /locus_tag="PLR57_05615"
     CDS             complement(211..1032)
                     /locus_tag="PLR57_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ40970.1"
                     /translation="MNVLITGASGGLGRALANECGKRGYNLFLVDINEQGLRAIQKGL
                     ERQFGVCVAAKACDLTSDASVDALMETIDERNIRFDMLLNVAGVDYEGGFLERTRKDV
                     VKIVSLNNEATLRITHAVLNRRRENKPFSLVFVSSLASMYPMPLKATYAASKRFLLDF
                     AMALRQELKSQDVNVLALCPGGLVTTKEAMSGIAAQGFWGGATTNPLETVARRTIGGV
                     LNGKDVYIPGALNRTLTFFGNLLPREWVAAAIYHRWNKAQQSWLKSELKEHIEAA"
     gene            complement(1029..1892)
                     /locus_tag="PLR57_05620"
     CDS             complement(1029..1892)
                     /locus_tag="PLR57_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQQ40971.1"
                     /translation="MNTVLITGATSGIGLASALELAKNGFRILGIGRDIKRCEQAKQR
                     ILSEVPGAEAEFFCADLMQQREVHRIADEIIAYLNENCGGELLALVNNAGCVRSWHTT
                     TEEGYEQQFALNHLAGFLLTHRMMPYLLKAKGRVLMTSSGSHRRMKMHWNDLMFRKGY
                     HPLLAYKQSKLCNVLFAKSLNDRFAWLGLHAYAVDPGLVCTEIGCKNTGGLVDFVWKH
                     RSKHGVNPDIPAKTYAYLCTTEPAPEGLYYYLCKEAPYSKEVNWENAGRLFRISERLC
                     GIQFDERTESK"
BASE COUNT          392 a          581 c          516 g          500 t
ORIGIN      
        1 cgtcgcatcc gggtacatgt ttattccaaa atgcttcgcg tgtaatgtgt tccacttcag
       61 aataatcctg cggcgtttcc agccggagtt cgatatcttg gcgcataaaa tgcttctcct
      121 cgttttgagc ggacgcaagt taaaaagttc gcgcgccgcc tctataagca taacgcgcga
      181 atatgaaata cgcaagaaaa tccaaacggt tcaggcggcc tcaatatgtt ccttcagttc
      241 gctcttgagc cagctttgct gcgctttgtt ccagcggtgg tatatcgccg cggcgaccca
      301 ttcccgcgga aggaggttgc cgaaaaatgt cagcgttctg ttcagcgcac cgggaatgta
      361 tacgtctttc ccgttgagta ctccgccgat ggtgcggcgg gcaaccgttt cgagcgggtt
      421 ggtcgtggcg ccgccccaga acccctgcgc ggcgattccg ctcatggctt cttttgtcgt
      481 tacaagtccg ccgggacaaa gcgcgagcac attgacgtcc tgacttttca actcttgccg
      541 cagcgccatg gcgaaatcca gcaggaatcg tttcgatgcg gcgtaggttg ccttgagcgg
      601 cattggatac atggaagcga ggctggaaac gaacaccagc gaaaacggct tgttctcgcg
      661 tctgcggttc agcaccgcgt gggtaatgcg gagcgttgct tcattattga gagaaacgat
      721 cttcacaaca tcctttcgcg tgcgctcgag aaacccgcct tcgtaatcca cgccggccac
      781 gttgagcagc atgtcgaagc ggatgttccg ttcgtcgatc gtctccatca gcgcgtcaac
      841 gctcgcgtcg cttgttaagt cgcaggcttt tgccgcgacg cacacgccga actggcgctc
      901 cagacctttt tgaatggctc tcagtccctg ttcattgata tccacgagaa aaaggttgta
      961 tcccctcttg ccgcattcat ttgccagcgc gcggcccagt ccgccgcttg cgcccgtaat
     1021 caagacattc atttgctctc cgtcctttca tcgaattgaa tcccgcagag ccgttcgctg
     1081 atgcggaaca gcctccctgc gttctcccag ttgacttctt tgctgtacgg cgcttccttg
     1141 cagaggtagt aatacagccc ctcaggcgcg ggctcggtcg tgcagagata tgcatacgtc
     1201 tttgcaggaa tgtccggatt cacgccgtgt ttactgcggt gcttccagac aaagtccacc
     1261 agcccgcccg tgttcttgca gccgatttcc gtgcagacaa gccccggatc aaccgcgtac
     1321 gcgtgcagtc ccagccaggc aaagcggtcg ttcaggcttt tagcgaacag tacgttgcag
     1381 agcttcgact gcttgtatgc caagagcgga tgataccctt ttcggaacat caggtcgttc
     1441 cagtgcatct tcatacgcct gtgcgaaccg ctggacgtca tcaacacgcg gccttttgcc
     1501 ttcaatagat acggcatcat gcgatgcgtc agtagaaatc cggcaaggtg attgagcgca
     1561 aattgctgct cgtagccttc ctcggtggtt gtgtgccagc tgcgcacgca acccgcgttg
     1621 ttgacgagcg caagcagctc accgccgcag ttttcgttga gatacgcaat aatctcatcc
     1681 gcaatgcggt gcacctctcg ctgctgcatc agatccgcgc agaagaactc cgcttccgcg
     1741 ccgggaactt cgctcagaat gcgttgcttc gcctgttcgc atctttttat gtcgcgcccg
     1801 attccgagga tgcggaatcc gttttttgcc aattcgagtg cacttgccaa accgatgcca
     1861 gaagtggctc cggtgatcaa tacggtattc attttatctc cttgtttatg acgattccgt
     1921 cgtcagccct tttctgttta agggcagtcc aaattgttcc tgttcgatca gcctgctgcc
     1981 agctgcgca
//
LOCUS       DAOMGY010000327         1979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_65715_length_1979_cov_3.367983, whole genome shotgun sequence.
ACCESSION   DAOMGY010000327 DAOMGY010000000
VERSION     DAOMGY010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65715_length_1979_cov_3.367983"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLR57_05625"
     CDS             <1..191
                     /locus_tag="PLR57_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40972.1"
                     /translation="SVALILPKAFTSFNWISGVMLLAGFVLSYLQTRYREKIEKKEAA
                     VLAVEAAQGTSSSENLEK"
     gene            450..>1979
                     /locus_tag="PLR57_05630"
     CDS             450..>1979
                     /locus_tag="PLR57_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HQQ40973.1"
                     /translation="MAATAGAFLYLVLKYYPETDFWFKGFVVFVFLYLVVFLAFAATY
                     RCFNIGTMRYRELTFAYILSTFLTNFIFYFILSLTAKKLLSVTPLLIMMLVQWAVSLL
                     LYFFADRLYYLLYPARKSIVVCSRNKHEINTFKKIDSMKERHEICAVVTEAEDVEAIK
                     RYLEPYSTVFMGDISRDLRLELTEYCFENNKRLFVLPTVEDIIFHNAHENIIGDSLVY
                     QCKNRTFSVEQLAAKRLMDIVISLIGIVITSPLMLLAAIVIKLQDGGPVFFRQERYTR
                     NLQRFTLYKFRSMIVDAEKDGAQFTRPGDSRITPFGKFMRASRIDELPQFFNILHGEM
                     SLVGPRAERIENVDFYCERMPEFRYRMKVKAGLTGYAQIYGKYNTNFEDKLKLDMLYI
                     ENCSLIHDLQLIFMTIRVLFMPESTEGFDISSLQELGSASCDVPSESASTAQAEQEGH
                     TTQMEARIKEFPAHKPGKSCYKGIILAGGKGTRLYPSTKAVSKQLLPIYDKPLVYYPL
                     SV"
BASE COUNT          449 a          527 c          530 g          473 t
ORIGIN      
        1 gctcggttgc gctgattctg ccgaaagcgt tcacgtcctt caactggatt tccggcgtga
       61 tgctgctggc gggctttgtg ctgagctatc tgcagactcg ctaccgcgaa aagattgaaa
      121 aaaaagaagc cgccgtgctg gcggtcgaag cggcgcaggg gacaagctct tccgaaaatt
      181 tggaaaagta agccaacgga cgaaactggc gtgaaaaagc gccggattcc ggcgaaaccg
      241 agcatagaat cgcgtcagcg cgaacgcgct gtggtttgac aatcctgttt cgcgcgtgct
      301 acaatcgatc cggcggagac tggtccggcg cgtttttttg atacgctcca acgccgattt
      361 ttttgcaaac gaaagaggaa cgcagggtgt cgaaatattc gcctaagcgc ttcaaaggct
      421 tattgatcat attttgcaaa ctggcgatca tggccgcaac ggccggcgcc tttttgtatt
      481 tggtgctcaa gtattacccg gagaccgact tctggttcaa agggtttgtt gtattcgtct
      541 ttctgtattt ggtcgtgttt ctcgcattcg cggctacgta ccgctgcttc aacatcggta
      601 ccatgcgcta ccgcgagctg acgtttgcct atattctctc aacgtttctg acgaacttta
      661 tcttctactt tattctctcc ctgacggcta aaaaactgct ttccgtaact cctttgctca
      721 taatgatgct ggtgcagtgg gcagtatcgc tgttgctcta tttcttcgcc gacaggctgt
      781 attatctgct gtatcctgcg cgaaagtcga ttgtcgtctg ctcgagaaac aagcacgaga
      841 tcaacacgtt caagaagatc gactccatga aggaacgcca cgaaatctgc gccgttgtga
      901 cggaagcgga ggacgtggaa gcgattaagc gctacttgga accgtattca acggtattta
      961 tgggcgatat ctcgcgggat cttcggctgg agctcacgga atactgtttc gaaaacaaca
     1021 agcggctctt cgtgctgccg acggttgagg acatcatctt ccataacgcg cacgagaaca
     1081 tcatcggcga ttctctcgtt taccagtgca agaaccgtac gttttccgtt gagcagctcg
     1141 cggcaaaacg gctgatggat atcgtcattt cgctcatcgg catcgtgatt acgagcccgc
     1201 tgatgctgct ggccgcgatc gtcatcaagc tgcaggacgg cgggccggtg ttctttaggc
     1261 aggaacgcta cacccgcaac ctccagcgct ttacgctcta taaattccgc agcatgatcg
     1321 tggacgcgga aaaggacggg gcgcagttca cccgtccggg cgacagccgc attacgccgt
     1381 tcggcaagtt catgcgcgct tcccgtatcg acgaactgcc gcagtttttc aacattctac
     1441 acggcgaaat gtcgcttgtc ggtccgcgcg cggagcggat cgagaatgtc gatttctact
     1501 gtgagcgcat gccggaattc cgctaccgca tgaaggtcaa agcgggactg acgggttacg
     1561 cacagatcta cggtaaatac aatacgaact ttgaggacaa gctcaagctc gacatgctct
     1621 atattgaaaa ctgttcgctg attcacgacc tgcagctgat ctttatgact attcgcgtgc
     1681 tgttcatgcc ggaaagcacg gaaggttttg atatctccag cctgcaggaa ctcggctcgg
     1741 caagctgcga cgtgccttct gaaagcgcct ctactgcgca ggccgaacag gaagggcata
     1801 caacgcagat ggaagcgcgc ataaaagagt ttccggcgca taagccgggg aaatcctgct
     1861 ataagggcat catcctcgcg ggcgggaagg gcacgcgcct ttaccccagc acgaaggccg
     1921 tcagcaaaca gcttctgccc atctacgaca agcctctggt ttactacccg ctctcggtg
//
LOCUS       DAOMGY010000328         1979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_65723_length_1979_cov_2.777027, whole genome shotgun sequence.
ACCESSION   DAOMGY010000328 DAOMGY010000000
VERSION     DAOMGY010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65723_length_1979_cov_2.777027"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1501
                     /locus_tag="PLR57_05635"
     CDS             125..1501
                     /locus_tag="PLR57_05635"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HQQ40974.1"
                     /translation="MRRKIAFLLTLWWMILCGAVGLCLLLFANKEAVVSEEENRTLAA
                     IPELTISQLAGGSFDEAFEDFLCDKFFLRTEIVDATSSLKHIFSALTVDELIDQTETD
                     EEAVMALLPQETAQEEPSTQETSAEITEQETEQEVQESTEPGSEPQAAQIVSGETPEQ
                     ANLWLLHADGTKTALLTYSKADIERAANVFNSYAALLPADGKMHVLLVPRSQTANKLA
                     LHLDTESGWSSDLESALEPLVSENVVVHSAVDILGSHITNGEYIFFRTDHHWTALGAY
                     YAANAMIQSEGYPTVPLADYASKSIEGFLGSIYLHGRSAKLKELADTLTYYEPLAPAE
                     SFWVSNTYRKSELKVIDTDQKDYKIFLGGTHGPYRVLDGGYHTGRNMLMVCDSFGNAI
                     APFLMPYYDSEYMVDFRDEYYSKEAAQGGVAEYIRRCGIQDIYVVLSESEGVGTLFIN
                     QLMPENLK"
     gene            1498..>1979
                     /locus_tag="PLR57_05640"
     CDS             1498..>1979
                     /locus_tag="PLR57_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40975.1"
                     /translation="MSEQGQQPHQVIHQPEENERKNATLIWRKHSAPVGGTAHAGDRA
                     FIRRALIGSLVAVVAFALLGYLAYRNSSTLKQAYAEVLITRGDFVKASKFIDNLNDET
                     AKAELLKKNFYAYAKKLDAENKLAEASALYLSAGDYEDSFQRWQDVTYRLAALYEADG
                     "
BASE COUNT          491 a          501 c          567 g          420 t
ORIGIN      
        1 tcaactgatc gagaccgttc tgttgaccgt ggtcgtcatt ttgagcattc tgaatcttgt
       61 cgcggacagc tataatccat ttttgtattt ccgcttttaa cgtttaggag gaaggagatg
      121 tcgtatgaga cgtaagattg cgtttttgct gacactttgg tggatgattc tctgcggcgc
      181 cgtcggcctc tgcctgctgt tgtttgccaa taaggaagcg gtcgtttcgg aggaagaaaa
      241 ccgcacgctc gccgccatac cggaactgac gatcagccag cttgcaggcg gctcgttcga
      301 cgaagcgttt gaggattttt tgtgcgacaa gttctttctg cgtacggaaa tcgtggacgc
      361 aacaagcagc ctgaagcaca tcttttccgc cttgacggtg gacgaactga tcgaccaaac
      421 cgaaacggac gaggaagcgg tgatggcgct tctgccgcag gaaacagcgc aggaagaacc
      481 ttccacacag gaaacatcag ccgaaataac agaacaagag actgagcagg aagtacagga
      541 aagcacggaa cccggttccg aaccgcaagc cgctcaaatc gtaagcgggg aaacaccgga
      601 gcaggcgaac ctctggctgc tgcatgcgga cggaacaaaa accgctctgc tgacgtattc
      661 aaaagcggat atcgagcgcg cggcgaatgt gttcaacagc tacgcggcac tcttgcccgc
      721 ggacggaaaa atgcatgtgc tgcttgtgcc tcgctcacag acagccaaca agctggcgct
      781 ccatttggat acggagagcg gctggtcgag cgatttggag agcgcgctgg aaccgctggt
      841 gagcgaaaat gtcgttgtgc attcggcagt ggatatcctc ggcagccata ttacaaacgg
      901 cgagtacatc ttcttccgca ccgaccatca ctggacggcg ttgggtgcat actatgcggc
      961 aaacgcaatg atccaaagtg aagggtaccc gacggtaccg cttgcggatt atgcttccaa
     1021 aagcatagaa ggatttcttg gctctatcta tctgcacggc cgcagcgcaa agcttaagga
     1081 gctcgccgac acgctgacgt attatgaacc tctcgcgccg gcggaatcgt tctgggtatc
     1141 caatacgtac cgcaaaagtg agctcaaagt gatcgatact gatcaaaagg actacaaaat
     1201 ttttctcggc ggaacgcacg gtccgtaccg cgtgctggac ggcgggtatc acacggggcg
     1261 caatatgctg atggtttgcg attcattcgg caacgcaatc gcgccgtttc tgatgccgta
     1321 ttacgacagc gaatacatgg tggacttccg ggacgaatat tacagcaaag aagcggcgca
     1381 gggcggcgtt gccgagtata tccgccgctg cggaatacaa gatatctatg tcgtactttc
     1441 cgaatcggaa ggggttggca ctttatttat caaccaactc atgccggaga atctaaaatg
     1501 agcgagcagg gacaacaacc gcatcaggtc atccatcagc ctgaggaaaa cgagcgaaaa
     1561 aacgctacgc tgatctggcg caagcattcc gcgcccgtcg gcggcacggc gcacgcgggc
     1621 gatcgcgcgt ttattcgccg cgcgctgatc ggttcgctgg ttgcggtagt ggcgtttgcg
     1681 ctgcttgggt atttggcgta ccgcaacagc tccacgctga agcaggcata tgcggaagtt
     1741 ctgattacgc gcggagattt cgtcaaggca agtaagttca ttgacaacct caacgatgaa
     1801 acagcgaagg cggagcttct gaagaaaaac ttttacgctt atgccaaaaa gctggacgct
     1861 gagaacaaac tcgccgaggc gagcgcgctg tatctatccg ctggggacta tgaagattcg
     1921 tttcagcgct ggcaggacgt aacctatcgt ttggctgcgc tgtacgaagc ggacgggga
//
LOCUS       DAOMGY010000329         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_66083_length_1972_cov_2.748044, whole genome shotgun sequence.
ACCESSION   DAOMGY010000329 DAOMGY010000000
VERSION     DAOMGY010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66083_length_1972_cov_2.748044"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLR57_05645"
     CDS             <1..510
                     /locus_tag="PLR57_05645"
                     /inference="COORDINATES: protein motif:HMM:NF027523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40976.1"
                     /translation="KIDGKKMYELARAGEDVEPKTRSVQIDALELLLHTGENRFLLKA
                     VCSRGTYIRSLCESIAERLDTIAYMPFLLRTRSGPFTIDQALSIGELESSKQEGTLER
                     AVISCEQAVSFLPEVRLPEDRRTPAKNGLETNLKGLANGYCSLYAGEEFLGVGLAEKG
                     CVKLRIHLY"
     gene            579..1472
                     /locus_tag="PLR57_05650"
     CDS             579..1472
                     /locus_tag="PLR57_05650"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HQQ40977.1"
                     /translation="MKKLTPAVVALGMFDGVHIGHKALISRAVEEANILRAEPAVFTF
                     SNHPMDVLGGSVKLLSSMKERNAMIRALGVQELVSEPFTCELAALEPEAFVDLLQARW
                     DVKAVVAGYNYTFGSRGLGTPETLERIGKERGFRVLVVPPVLYEGEPVSSTRVREAVE
                     RGDVALAGALLGRMYSLSGRIIRNKRIGRRIGFPTANIEPEERRVLPPDGVYATIAYV
                     RGAAYRAVTNIGTNPTIEDGKLGIETHLLDFDADIYGEVLTVAFHSFLRGEIKFKSVE
                     ELKEQIKLDVQKAAAIKQVAL"
     gene            1651..1908
                     /gene="rpsO"
                     /locus_tag="PLR57_05655"
     CDS             1651..1908
                     /gene="rpsO"
                     /locus_tag="PLR57_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HQQ40978.1"
                     /translation="MEKQDIIVKYALHEGDTGSPEVQIALLTERINHLNEHLKLHPKD
                     HHSRRGLLKMVGQRRGLLNYLKEKDIERYRTIAASLNIRIK"
BASE COUNT          460 a          519 c          601 g          392 t
ORIGIN      
        1 aagatcgacg gaaagaagat gtacgaactc gcgcgcgcgg gggaggacgt tgaacccaag
       61 acccgttccg tgcagataga cgcgctggaa ctcttgctgc atacaggcga gaaccggttt
      121 ctgctcaagg cggtctgctc gcgcggcacc tacatccgtt cgctctgcga aagcattgcc
      181 gagcggctgg atacgattgc ttatatgccg tttcttttaa ggacgcggag cggcccgttt
      241 acaatagatc aggctttgag cattggcgag ctggagagca gcaagcagga aggcacgctg
      301 gaacgcgcgg tgatctcctg cgagcaggcg gtttcgtttc tgccggaagt gcgcctgccg
      361 gaggatcgca gaacgcccgc gaaaaacggg ctggaaacga atctgaaagg actggcaaac
      421 ggttactgca gtctgtacgc gggggaggaa ttcctgggcg taggactggc ggaaaaaggg
      481 tgcgtcaagc tccggattca cttgtattga ttgacggaaa gcgcgtaagg aggttagctc
      541 ccgtcgccga accgttttag ctggaaggta ccgattgtat gaaaaagctc actcccgccg
      601 tcgtggctct cggcatgttc gacggcgtgc atattggcca caaggcgctc atcagccgcg
      661 cggtggagga ggcgaacatt cttcgcgccg aacccgcggt atttacgttt tccaaccatc
      721 ccatggacgt gctgggcggc agcgtcaagc tgctctcgag catgaaggag cgaaacgcga
      781 tgattcgcgc gctcggcgtt caggagctcg tcagcgaacc ctttacctgt gagcttgccg
      841 cgctggagcc ggaagcgttt gtggaccttc tgcaggcgcg ctgggacgtt aaagcggtag
      901 tagcggggta taactacacc tttggcagcc gggggctcgg cacgccggaa acgctggaac
      961 gaatcggcaa agagcgcggc tttcgcgtgc tcgtcgtgcc gccggtgctc tatgaaggcg
     1021 agccggtttc cagcacgcgc gtgcgggaag ccgttgagcg cggggacgtg gcgcttgccg
     1081 gagcgcttct gggacgcatg tattcgcttt ccggcagaat catacgcaac aaacgcatcg
     1141 gcagaagaat cggttttccc accgcgaata tcgagccgga agagcggcgc gtgcttccgc
     1201 cggacggcgt ttacgcaacc atcgcatacg tgcggggcgc cgcttaccgc gcggtgacca
     1261 acattggcac aaacccgacg atagaggacg gaaaactggg aatagaaacg catcttcttg
     1321 actttgacgc ggatatctac ggcgaagttc tgacggtggc gttccacagc tttttgcgcg
     1381 gggaaatcaa atttaaaagc gtggaagagc tcaaggaaca gattaagctg gatgtgcaaa
     1441 aggccgccgc gatcaagcag gtcgccctgt aaatgctctc gagtacattc cgccgcgctg
     1501 tgaacggagc gaaaagtttt gtctgccgct gtttacaagc gaagtgagat atgctatagt
     1561 acataggtaa ttttatggcc gtttaccggg cagagcgacg ctccgacgtt ccgactcagt
     1621 ttacggcgaa tagaaaaaag gagaaagaca atggaaaaac aggatatcat cgtaaaatac
     1681 gcactgcacg aaggcgatac cggatccccc gaggtacaga tcgctctgtt aaccgagcgc
     1741 atcaaccacc tcaacgagca cctcaagctc cacccgaaag accatcactc ccgccgtggg
     1801 ctgctcaaaa tggtcggtca gcgcagaggt ctgttgaact acctcaaaga gaaggatatc
     1861 gagcgttaca gaacgattgc tgcaagcctg aacattcgta tcaaataacg ggagaggcgg
     1921 gctgttcccg cctttctttt tattgctaca cgggcctgtc tgttcccgct at
//
LOCUS       DAOMGY010000330         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_66171_length_1970_cov_4.589556, whole genome shotgun sequence.
ACCESSION   DAOMGY010000330 DAOMGY010000000
VERSION     DAOMGY010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66171_length_1970_cov_4.589556"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..747)
                     /locus_tag="PLR57_05660"
     CDS             complement(1..747)
                     /locus_tag="PLR57_05660"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40979.1"
                     /translation="MIKRWQKTLNVINRLIELVLIFASYFGSVAFWLLLIRHDPENIA
                     VQLSSAGWYAFFYAVLIVLCYQLVGLYDSVRARPIGNEIRWVLSVNALITLLGAALLY
                     VFRLTNFSRGVILSIYVISCLLILGKRLAVRIVLSFYRSKGYNQKHVILIGSGPLARK
                     YMETIRRTSRYGYTIDGYIGNSKTMTEIPYLGTWEEVGSRIIEQSDADEIVAALDQAN
                     LSLLPKIIAATENDLPPFYGSAAMLVNELF"
     gene            complement(1019..>1970)
                     /locus_tag="PLR57_05665"
     CDS             complement(1019..>1970)
                     /locus_tag="PLR57_05665"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQQ40980.1"
                     /translation="TGKTAAFVIPIVERIQPENRKVQALILCPTRELALQTTSVIKKL
                     SAHLPIRTVSVYGGQSPNVQLQALRQGAQIVVGTPGRVKDFIGQNALKLSNVRFVVLD
                     EADEMLDFGFLPDIKSILSYVTGEHQTMLFSATMSKEISVIAEKYLVNPLRVEVGKRN
                     EPTKTVRQMCVHTAEAGKIVTVGQLMEQTSPRLTLIFCNTRHRVKSVTKKLLASGTAT
                     ACLHGDLSQNQRNSIMREFRAGKTPVLVATDVAARGIDVDNIDLVINYDVPDKPEYYV
                     HRIGRTGRAGHSGLACTLVSEQDSGRLQTIKRQYNVLMAQ"
BASE COUNT          423 a          506 c          601 g          440 t
ORIGIN      
        1 ttagaacaat tcatttacta acattgctgc tgaaccataa aacgggggca gatcattttc
       61 cgttgcggcg atgatctttg gcaaaagaga aaggttcgcc tgatccagcg cggctacgat
      121 ttcgtccgcg tccgactgtt caatgatgcg gctgccgact tcttcccagg tgcccaggta
      181 cgggatttcg gtcatggtct tggagttgcc gatatatccg tctatggtat agccatacct
      241 tgaggtgcgg cgaatcgttt ccatatactt tcgcgcaaga gggccgctgc cgatcaggat
      301 gacgtgcttc tggttatagc ctttgctgcg gtaaaacgac agcacaatac gcaccgcgag
      361 gcgtttgcca aggatgagca ggcaggagat aacgtatatg gagaggatga cgccgcgcga
      421 gaagttggtc agccggaaca cgtacagcag cgccgcgccg agcagcgtaa tcagcgcgtt
      481 gacgctgagc acccacctga tttcgttgcc gatgggtctt gcacgaacgg agtcgtagag
      541 cccaaccagc tggtagcaca atacgatgag cacggcgtaa aaaaacgcgt accagcctgc
      601 ggaagagagc tgcaccgcga tgttttccgg atcgtgacga atgagcaaaa gccagaaagc
      661 aaccgaaccg aagtacgacg cgaagatcag taccagttcg atcagccggt tgattacgtt
      721 cagcgtcttt tgccagcgtt taatcatgtg aaacctcttt ttcgttgggt atctctcgtg
      781 tatgcgcaat ttccaacacg gatttcagga aagcaggctg cagaaggcgg ttttgcatcg
      841 gggaagagaa gaaccgcctc ccgctctatt ttagtattga accgcacaaa cgtcaacgat
      901 aacgcgcggc atttgttccg tatagaaggt aaaatcttgt gcaaaaatca gctaatgaaa
      961 cagctgtgcg caaataaaaa tgccgcccgg ggatcgggcg gcattgcgtt tataccgttt
     1021 actgcgccat cagcacgttg tactggcgct tgatggtctg cagacgaccg gaatcctgct
     1081 cgctgaccag cgtgcaggca agaccggagt ggcccgcgcg accggttctg ccgatgcggt
     1141 ggacatagta ttccggcttg tccggcacat cgtagttgat cacgagatcg atgttgtcca
     1201 cgtcgatgcc gcgcgcggct acgtcggttg caacgagcac gggggtttta cccgcgcgga
     1261 attcgcgcat aatgctgttg cgctggttct gcgaaaggtc gccgtggagg caggcggtcg
     1321 ccgtgccgct ggcgaggagc tttttggtta cggattttac cctgtgccgt gtgttgcaga
     1381 agatcagcgt cagccgtggg gaagtctgct ccatgagttg gccgacggtg acgattttgc
     1441 ccgcttccgc ggtatgcacg cacatctgcc ggacggtctt ggtcggttcg ttgcgcttgc
     1501 cgacttcgac gcgcagggga ttgacaaggt acttttcggc gatgacggag atttccttgc
     1561 tcatggtcgc ggaaaagagc atggtctggt gctcgcccgt gacataggaa aggatggact
     1621 tgatgtccgg caaaaagccg aaatcgagca tttcgtccgc ttcgtcaaga acgacaaacc
     1681 gaacgttgct gagcttgagc gcgttctggc cgataaagtc ctttacgcgg ccgggcgtgc
     1741 cgacaacgat ctgcgcgccc tgacggagcg cctgcagctg gacgttgggt gactgcccgc
     1801 cgtaaacgct gacggtgcgg atgggcaggt gcgcggagag ctttttgata acgctcgtgg
     1861 tctgcagcgc gagttcacgc gtggggcaga ggatgagcgc ctgcacctta cggttttccg
     1921 gctggatgcg ctccacgatg gggatgacga acgccgccgt tttgccggtg
//
LOCUS       DAOMGY010000331         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_66237_length_1969_cov_3.212644, whole genome shotgun sequence.
ACCESSION   DAOMGY010000331 DAOMGY010000000
VERSION     DAOMGY010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66237_length_1969_cov_3.212644"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLR57_05670"
     CDS             <1..217
                     /locus_tag="PLR57_05670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ40981.1"
                     /translation="VGSIAMQAMIDFARSTGVLRTLSLEVREGNERAISLYKRFGFVE
                     IGRHKGRINVRGTYYDEILMDLDLTQA"
     gene            278..1048
                     /locus_tag="PLR57_05675"
     CDS             278..1048
                     /locus_tag="PLR57_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQQ40982.1"
                     /translation="MKLFDTHAHLLDAQFDHDRNEIIPALPEHGVAGFLEACTEAGDI
                     PKITALVMLYDHVFGSAGIHPHSADSANDANLLIVERALKQPKILAVGEIGLDYHYDF
                     SPREVQREAFAAQLDMAIAAKKPVIIHDREAHGDVMSLLSERCGRLNGVLHCYSGSYE
                     DAVRYIDMGYHIAFGGALTFKNAVKQRAIAEKLPLERIVIETDCPYMTPEPHRGTRND
                     PRLMHLTLEMLASVRGISAEAAAEATFANAMQLYGLEL"
     gene            complement(1158..1799)
                     /locus_tag="PLR57_05680"
     CDS             complement(1158..1799)
                     /locus_tag="PLR57_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40983.1"
                     /translation="MSDYSLSALSILRNPQLTLKWYVVPLLLIVMYIYTKEAHEKNWN
                     VILGGIALWGMDLFNEIWNSIVFHATNFAPVWGTPMGVGDTALLILIGYNIEISFMFA
                     MMGIMACLSLPKDPKQKILGVNNRVFLAVLFTTLAVIVECFLNYSGLLTWEYPWWSLK
                     CPYLIWLIGYLPFFTMAFVVHDMKKMKNKFIALGVIFGVDIILLVVFGLMGWM"
BASE COUNT          480 a          568 c          539 g          382 t
ORIGIN      
        1 cgtcgggagc atcgccatgc aggccatgat cgactttgcc agaagcaccg gcgttctgcg
       61 cacgctctcg ctggaagtgc gcgaaggcaa cgaacgcgcg atttcgctct acaagcgctt
      121 cgggttcgtc gagatcgggc ggcacaaggg gcgcatcaac gtccgcggaa cgtactacga
      181 cgaaatcctg atggatctgg atttaacgca ggcgtaacag tccactcctg cgcggcaggc
      241 gtaaaaaaca cagcagactg gaaacggaac ataagaaatg aagctattcg atacccacgc
      301 gcacctgttg gatgcgcagt ttgaccacga ccgcaacgaa atcatccccg ccctgccgga
      361 gcacggcgtt gcggggtttc tggaagcctg cacggaagcg ggcgacatac cgaaaatcac
      421 cgcgctggtg atgctctatg atcacgtgtt cggcagcgcg ggcatccatc cgcattcggc
      481 agacagcgca aacgacgcaa acctcctgat cgtggagcgc gcgctcaagc agccgaaaat
      541 cctagccgtc ggcgagatcg ggctggacta tcactacgat ttttcgccaa gggaagtaca
      601 gcgcgaggcc tttgccgcgc agctggatat ggccattgcc gcgaagaagc ccgtcatcat
      661 ccacgaccgc gaggcgcacg gggacgtgat gtcgctgctc tccgaacggt gcggcaggct
      721 gaacggtgtg ctgcactgct attccggaag ctacgaggac gcggtgcgct atatcgacat
      781 gggctaccac atcgcctttg gcggcgcgct gacgttcaag aacgccgtca agcagcgcgc
      841 aatcgccgag aaactcccgc tggagcgaat cgtaatcgaa acggactgcc cgtacatgac
      901 gcccgaacca caccggggca cccgcaacga tccgcggctc atgcacctga cgctggagat
      961 gctggcgagc gtgcgcggaa tctccgccga ggcagccgcc gaagcaacgt ttgccaatgc
     1021 gatgcagctc tacggactag aactttagat tcgagggctc cgccctcgag ctcctgccaa
     1081 agggacggtc gtccctttgg aaaccctata tataacaaaa gccccgccgg aaggcggggc
     1141 ttttgcttca cttttcctta catccaaccc atgaggccga agacgaccag caaaatgata
     1201 tccacgccga agatgacgcc tagagcgatg aacttgttct tcatcttctt catatcgtgc
     1261 acgacaaacg ccatcgtaaa gaacggcagg taaccgatca gccagatcag gtacgggcac
     1321 ttcaggctcc accacggata ctcccaggtc agcagaccgg agtagttcag gaagcattcc
     1381 acaatgaccg caagcgtcgt gaacagcacg gcgaggaaca cgcggttgtt gacgccgagg
     1441 atcttctgtt tcgggtcctt cggcagcgag aggcacgcca taatgcccat catggcgaac
     1501 atgaacgaaa tttcaatgtt gtagccaatc agaatcagca gcgccgtgtc gccaacgccc
     1561 atcggcgtgc cccagacggg cgcaaagttg gtcgcgtgga aaacgatgct gttccagatt
     1621 tcgttgaaca aatccatgcc ccacagcgcg atgccgccga ggatgacgtt ccagttcttt
     1681 tcatgcgctt ctttggtgta gatatacatc acgatcaaga gcagcggcac aacgtaccac
     1741 ttgagcgtga gctgcgggtt gcgcaaaatg gataatgcgc tgagcgaata gtccgacatg
     1801 atttgcctcc ccttatgcaa tttgaaatgt acgattatag aatacgcttt tatgatactt
     1861 taatcaacac ctgttgcgga tttcaatgtg gggatcactt ttttaagctt taatcaacac
     1921 ctgttgcgga attcacaaag cgaaactccc tcttttcgcg caaaatgga
//
LOCUS       DAOMGY010000332         1964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_66496_length_1964_cov_3.484547, whole genome shotgun sequence.
ACCESSION   DAOMGY010000332 DAOMGY010000000
VERSION     DAOMGY010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66496_length_1964_cov_3.484547"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="PLR57_05685"
     CDS             complement(<1..163)
                     /locus_tag="PLR57_05685"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQQ40984.1"
                     /translation="MRILVVNGPNLNLLGLREPEIYGSRTYADLLELIRAEAELLGAE
                     VSFVQSNHEG"
     gene            complement(144..1388)
                     /locus_tag="PLR57_05690"
     CDS             complement(144..1388)
                     /locus_tag="PLR57_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HQQ40985.1"
                     /translation="MGNQIYGLLGRSLKHSHSVFIHRELENGSYRLFELEPEELNTFL
                     RRDDIGGLNVTIPYKRDVIPFCDTLDETARAVGSVNTIVRGHDGKLVGYNTDVYGLAC
                     MAERAGISFAGRKVVVFGSGGASLTAQYAAKNGGAKEVVVISRKGPNSYETLSRHADA
                     ELLVNATPVGMYPNNGALPADPADFPACKGVLELVYNPLRTALTLRAQSLGIPVSDGL
                     IMLTAQAKAAEELFWDQKLPESEIERIDLLLKKQTQNIVLVGMPGSGKSSVGEALGRL
                     SGRETVDFDREIELTAGKPIPQIFFESGEEAFRELERQITQKWGAEQGRILVTGGGVV
                     KDERNYAPLKQNGRIYQIERETALLRREGRPLSERADLAAMQRERQPLYERFRDASAQ
                     NGVSAEHTARNIWEEYNANFSR"
     gene            complement(1392..>1964)
                     /locus_tag="PLR57_05695"
     CDS             complement(1392..>1964)
                     /locus_tag="PLR57_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HQQ40986.1"
                     /translation="IRHELMAKPGTKAADITEIFSHEQALGQCGRWLATLSGKVKITP
                     CANTALAAKMVAESADPGAAAICSHACAELYGLETLSGDIQDSDNNYTRFICVTKKPA
                     IYAGANRISLILACQNKPGALNEILTKLAAHGVNMSKLESCPVTGGNFEFEFFMELDA
                     SVREPGVLPLLEELERISESFLFLGNYSMV"
BASE COUNT          334 a          596 c          610 g          424 t
ORIGIN      
        1 cgccctcgtg gttggactgc acaaatgata cctccgcgcc gagcagctcc gcttccgcgc
       61 ggatgagttc cagcaggtcg gcataggtgc ggctgccgta gatttccggc tcgcgaagtc
      121 ccagcagatt caggttcggt ccgttaacga ctaaaattcg cattgtattc ctcccagata
      181 ttccgcgccg tgtgctcggc ggaaacgccg ttttgcgcgc tcgcgtcgcg gaaacgctcg
      241 tagagcggct ggcgttcccg ctgcatggcc gcgaggtcgg cacgctccga gagcgggcgg
      301 ccttcgcgcc ggagcagggc ggtttcccgc tcgatttggt agatgcgccc gttctgcttg
      361 agcggcgcgt aattccgttc gtccttgacg actcctccgc cggtgacgag aatccgtccc
      421 tgttccgcgc cccatttctg cgtgatctgg cgctcgagct cgcggaatgc ctcctcgccg
      481 gattcaaaaa agatttgcgg aatcggtttt cccgccgtaa gctcgatttc gcggtcaaaa
      541 tcgaccgttt cgcgcccgga caggcgaccg agcgcttccc cgacgctgga ctttccgctg
      601 cccggcatgc cgacgagcac gatgttctgc gtttgctttt tcaggaggag atcgattcgc
      661 tcgatctcac tttcgggcag cttctgatcc caaaagagtt cttccgccgc tttcgcctgc
      721 gccgtgagca tgatcagtcc gtccgaaacg gggatgccca gcgactgcgc gcggagcgtc
      781 agcgcggtgc ggagcggatt gtacacaagc tccagcacgc ccttgcacgc gggaaaatcc
      841 gcggggtcgg ctggcagcgc gccgttgttt gggtacatgc cgacgggggt tgcgttgacg
      901 agcagttccg cgtccgcgtg gcggctgagc gtttcgtagg agtttggccc cttgcgggag
      961 atcacgacaa cttcttttgc gccgccattt ttagcggcat actgcgccgt aagcgaagcg
     1021 ccgccgctgc cgaagacgac gacctttcgc ccggcaaagg agatacccgc gcgctctgcc
     1081 atgcaggcca gcccgtacac gtccgtatta tacccgacca gtttgccgtc gtggccgcgg
     1141 acgatcgtat tgacgctgcc gacggcgcgc gccgtttcat ccagcgtgtc gcaaaaggga
     1201 attacgtccc gcttgtacgg aatggtaacg ttcaggccgc cgatatcgtc ccgccggaga
     1261 aacgtgttta gctcttccgg ctccagctca aacaggcggt agctcccgtt ttccagttcg
     1321 cggtggatga aaacggagtg gctgtgcttg agcgagcggc caagaaggcc gtaaatctgg
     1381 ttgcccacgg cttacaccat cgagtagttg cccagaaaga ggaacgactc gctgatgcgc
     1441 tcaagctcct ccaaaagcgg cagtacgccc ggctcgcgaa cggaagcgtc cagctccata
     1501 aagaattcga attcaaagtt gccgcccgtc acgggacagc tttcgagctt gctcatgttt
     1561 acgccgtgcg ccgcgagctt agttaaaatt tcgttgagcg cgccgggttt gttctgacag
     1621 gcgagaatca ggctgatgcg gtttgcgccc gcgtagatgg cgggtttttt agtcacgcag
     1681 atgaagcgcg tgtagttgtt gtcgctgtcc tgaatgtcgc cgctgagcgt ttcgagcccg
     1741 tagagctcgg cgcaggcgtg cgagcaaatg gccgcggcgc cgggatctgc gctttcggca
     1801 accatcttgg ccgcgagcgc cgtgttggcg cagggggtaa tctttacctt gccgctgagc
     1861 gtggcaagcc agcgtccgca ctggccgagc gcctgctcat gcgaaaagat ctccgtaatg
     1921 tcggctgcct tcgtgccggg ttttgccatg agctcgtggc ggat
//
LOCUS       DAOMGY010000333         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_66557_length_1963_cov_3.261006, whole genome shotgun sequence.
ACCESSION   DAOMGY010000333 DAOMGY010000000
VERSION     DAOMGY010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66557_length_1963_cov_3.261006"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLR57_05700"
     CDS             complement(<1..298)
                     /locus_tag="PLR57_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HQQ40987.1"
                     /translation="MEAKGQESINVKPNEYFGSLNERAARIRARLLETQPSVCTERAV
                     LTTQAYRAHEQDQVVLRRAYMLDQVLRNMTVYIDPDALLAGNQAVFRFGSIELVT"
     gene            complement(303..1247)
                     /locus_tag="PLR57_05705"
     CDS             complement(303..1247)
                     /locus_tag="PLR57_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQQ40988.1"
                     /translation="MRNHYVIGIDAGGTKVAYGLFGESGELLDRMQHPTDICAGGEAF
                     SDTVAENILALLSRNSLNEADLDGVGICMPSFILFDEGRILMTSAMPGIKNFPMRDYL
                     SERIHTRIVLDNDSNAAALAEYRRGAGRGSRHLVYITAGTGLGSGIVIDGKLFRGSYG
                     WAGECGHMLATPDEGLLCGCENRGCFMSYASGRSMTARVRGQMSYCHATSLTPETADG
                     EHILAAFREGDPLAKEMIETTAHYLGVCAFNIYQMLNINRFVFGGGLTHFGDVLFDRV
                     KAEFDKYNHIPLPVEFRLAELGDDIGLIGAAEFVRGDS"
     gene            1631..>1963
                     /locus_tag="PLR57_05710"
     CDS             1631..>1963
                     /locus_tag="PLR57_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HQQ40989.1"
                     /translation="MKRSEINHAIRRMEAFAKLHSFELPPFCAFAPEEWPNKNHEYDE
                     IRDNMLGWDVTDYGAGDFAKMGFSLVTIRNGSLANREKYPKSYAEKLLYLEETQYSPM
                     HFHWSKMED"
BASE COUNT          440 a          546 c          535 g          442 t
ORIGIN      
        1 tggtgacgag ctcgatcgag ccgaagcgga acacggcctg gttgccggcg agcagcgcgt
       61 ccgggtcaat atagacggtc atattgcgca gcacctgatc gagcatatac gcgcggcgga
      121 gcacaacctg atcctgctcg tgcgcgcggt aggcttgcgt ggtcaaaacg gcgcgctcgg
      181 tgcagacgct cggttgggtt tccagcaggc gcgcgcgtat gcgggcggcg cgctcgttga
      241 gcgaaccgaa atattcgttc ggttttacgt tgattgattc ctgacctttg gcttccataa
      301 atttaagaat ctcctctgac aaactccgcc gcgccgataa gaccgatatc gtcccccaat
      361 tccgcaagcc tgaactccac cggcagcgga atgtggttgt atttgtcgaa ttccgccttg
      421 acgcggtcaa acagaacgtc cccgaaatgg gtcagcccgc cgccgaagac aaagcggttg
      481 atgttgagca tctggtaaat attgaacgcg cagaccccga ggtaatgcgc ggtggtttcg
      541 atcatttcct ttgccagcgg gtcgccttcg cgaaacgccg cgaggatatg ctcgccgtcg
      601 gcggtttcgg gcgtgaggct cgttgcatgg cagtagctca tctgcccgcg tacgcgcgcg
      661 gtcatgctgc gcccggacgc gtaggacata aagcagcccc ggttttcaca gccgcagagc
      721 aggccttcat cgggagtggc gagcatatgc ccgcattccc ccgcccagcc gtagctgccg
      781 cgaaaaagtt ttccgtcgat gacgataccg ctgcccagcc ccgtacccgc cgtaatgtaa
      841 acaaggtgcc tgctgccgcg ccccgcgccc cggcggtact ccgccagcgc cgcggcgttg
      901 ctgtcgttat ccagcacgat gcgggtatgg atccgctcgg aaagatagtc ccgcatagga
      961 aaattcttga tgcccggcat ggcggacgtc atgagaatcc tgccttcgtc aaagaggatg
     1021 aacgacggca tgcagatgcc aacgccgtcg agatcggctt cgtttaaact gttgcggctg
     1081 aggagagcca ggatgttttc cgcaaccgtg tccgaaaacg cttctccgcc cgcgcagata
     1141 tccgttgggt gctgcatcct gtcgagtagt tcacccgatt cgccaaaaag cccgtaagct
     1201 accttggttc ctccggcgtc gatgccgatg acgtaatgat tgcgcatgca ctgttccttt
     1261 cacataaaaa ggttcggttt ttgccgcgaa agcgcgcttg ccgacaggtt cagtatagta
     1321 ttgcaatggt gcaatgtcaa gaaaaaccgt tcgtttgata gcatcattgc tttcatatga
     1381 aactttattc gcgatgattg caaatttgag cgcaatcctg tatgataagg gcgcgaaagc
     1441 gcgtatccgc gcgaacgtgc tccttttctg ccgttttgta tgcaatttcc attttctttt
     1501 tgtaattcag ggcgttttct ttccgagcaa aacgttccgg tatgataaat acattgtctg
     1561 caggaaggac tcttattaaa tgaagcattc cgaaatcaaa aaaatcatgt ttcaggagaa
     1621 tatgaactaa atgaaacgtt ccgagattaa tcatgccatc cgccgcatgg aagcgtttgc
     1681 caagctgcac agtttcgagc tgccgccgtt ctgcgcgttt gcgcccgaag agtggccaaa
     1741 caagaaccac gaatacgacg aaatccggga caatatgctc ggctgggacg ttaccgacta
     1801 cggcgcgggc gactttgcta aaatgggctt ctcgctcgtg accatccgca acggcagcct
     1861 tgccaaccgg gaaaaatacc cgaaatccta cgccgaaaaa ctgctctacc tcgaagaaac
     1921 gcagtattcg cccatgcact ttcactggag caagatggag gat
//
LOCUS       DAOMGY010000334         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_66563_length_1963_cov_2.785115, whole genome shotgun sequence.
ACCESSION   DAOMGY010000334 DAOMGY010000000
VERSION     DAOMGY010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66563_length_1963_cov_2.785115"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1346
                     /locus_tag="PLR57_05715"
     CDS             360..1346
                     /locus_tag="PLR57_05715"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HQQ40990.1"
                     /translation="MEYNNQTSKSVENSAWLILLLAVAAGAAALIIPFGLIVVPALWA
                     YAGARTKPGWIALPAAVYALGALMLYSENGYTSAVAVSVMAAASAVALYVLLTKRFSN
                     TYTALTLSGIFLLGLYVAACLPSVLAGKGAFAAVQAAMDEIISFYRTTFVQAAGADAQ
                     YAALVNEYLDTFSKAVPTMVVAALSIFAGVMGLGNLLFFRLFCRKHKEIAISPIREFR
                     FWSLPRSMMFGLFALLVGSLILEWSGWQYAESLVNTVNVLVGMPLLLQGLAVVDFLIV
                     RSARKTAGQRAVMYVLIGVLFGLAEMPLMLLGGFEQLFHFRARTQGLPPQSL"
     gene            1442..>1963
                     /locus_tag="PLR57_05720"
     CDS             1442..>1963
                     /locus_tag="PLR57_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40991.1"
                     /translation="MKNAKTCLLPIGIFAALLCVLLFFSGAIQSVLLPISGMALGVVI
                     LLLVICRGVELNWARERQKQMDDVFSENASLRTELIDRVAIPCALIESSGKIVWRNEM
                     LKKLYEDADIRLIQPNFDFSAPLVAAPMELSGSVYQVMSIPAHREKDDRELVFQYWVD
                     RTEAAHYQRLFEEQ"
BASE COUNT          356 a          538 c          582 g          487 t
ORIGIN      
        1 tgaggtcgcc gttcgtccag ggcatggaca gcaggaacag ccgcatcgga ctggtcctca
       61 cggtcctgat gctgctgttg atgtacctgc tgtccgcgtg gcaggacaat ctggacgaga
      121 gtttcaccca cgccgcgtac gggctgctct ttttagggac gctgttcaaa ggcctgtaaa
      181 aaggcgaaac agggcataaa ccggcgcgca gacagtatgc gcgccggttt tttcgctttt
      241 gacagttttc gccgctacgg gcattttctg ttcccgtatt ggtgcggata gaacagaaaa
      301 tattctttcg ctcgtcattc ttcattgacc atttgccgta cgggcattaa aatgatgtca
      361 tggaatataa taatcaaacg tcaaagagcg ttgaaaacag cgcctggctc attttgctgc
      421 ttgctgtggc tgcgggcgcc gccgctctga ttatcccgtt cggcctgatc gttgttcccg
      481 cgctctgggc atatgccggc gcgcggacaa aacccggctg gattgcgctg cccgcggcgg
      541 tttacgcctt gggagctctg atgctctatt ccgaaaacgg gtatacatcc gccgttgcgg
      601 tttctgtgat ggcggcagcc agcgcggttg cgctgtatgt tctcctgaca aagcgcttca
      661 gcaacaccta taccgcgctt acgctttccg gcatcttttt actgggattg tatgttgcgg
      721 cgtgcctgcc cagcgtactg gcgggtaaag gcgcgtttgc cgcggtgcag gccgccatgg
      781 acgagattat ttcgttttac cgcacgacgt ttgtgcaggc cgcaggtgcg gacgcgcagt
      841 atgccgcgct ggtcaacgag tatctggata cgttttcgaa ggcggtgccg acaatggtcg
      901 ttgccgcgct cagcatcttt gccggcgtaa tggggctggg caacctcctg ttcttccggc
      961 tgttttgcag aaagcacaag gagattgcca tttcacccat ccgggaattc cggttctggt
     1021 ctctgccgcg cagcatgatg tttggattgt ttgcgctgct cgtaggctcg ctgatcctcg
     1081 aatggtcggg ctggcagtac gccgaaagcc tggtcaacac ggtcaacgtg ctggtcggta
     1141 tgccgctgct gctgcagggc ctcgccgtgg tggattttct catcgttcgt tccgcgcgta
     1201 aaacggcggg acagcgcgcg gtaatgtatg tgctcatcgg cgtactgttc gggctggcgg
     1261 agatgccgct gatgctgctc ggcgggtttg aacagctctt tcacttccgc gcgcgtacgc
     1321 aaggtttgcc gccgcagtcg ctgtaaccca tatcgcaaga ctttttccgc gccggtgcaa
     1381 acgcttgaaa gaatcctttc cgtttgcatg tgcgtttcat tcaatctccg ggaggtgcaa
     1441 aatgaagaac gctaaaacct gtctgctgcc gatcggcata ttcgcggcgc tgctgtgtgt
     1501 tttgctgttc ttctccggag cgattcagtc cgtgctgctg ccgatatccg gcatggcgct
     1561 cggcgtggtt atcctgctgc tggtcatctg ccgcggcgtg gagctcaact gggcgcgcga
     1621 acggcagaag caaatggacg atgtgttttc cgaaaatgcc tcgctgagaa ccgaactgat
     1681 cgaccgcgtt gcaattccct gcgcgctcat tgaatcgagc ggcaagatcg tctggcgcaa
     1741 cgagatgctc aaaaagcttt acgaagacgc ggatattcgc ctgattcagc caaacttcga
     1801 cttttccgcg ccgctcgttg cagcgcccat ggagctctcc ggcagcgttt atcaggtgat
     1861 gagcataccc gcccaccggg agaaggacga ccgcgaactg gtgtttcagt actgggttga
     1921 ccgcaccgag gcggcgcatt atcagcgctt gtttgaagag cag
//
LOCUS       DAOMGY010000335         1962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_66619_length_1962_cov_2.716308, whole genome shotgun sequence.
ACCESSION   DAOMGY010000335 DAOMGY010000000
VERSION     DAOMGY010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66619_length_1962_cov_2.716308"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLR57_05725"
     CDS             complement(<1..226)
                     /locus_tag="PLR57_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ40992.1"
                     /translation="MKTLFNLTTSPNDMERFSTRAELLALMEGFDGVELLACEQDERN
                     IVPKERVFGIHMGYFPYWLDFWCGNEAALIQ"
     gene            complement(320..958)
                     /locus_tag="PLR57_05730"
     CDS             complement(320..958)
                     /locus_tag="PLR57_05730"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQQ40993.1"
                     /translation="MEIILLRHGKTAANLDNRYNGRTDDPLCEEGLREANSAQKHASF
                     THVYVSPLLRARQTAGICFPNAEQIVVNDLREMNFGDFEGRTADEMEHDSAYRAWVES
                     NGMEACPNGESVPQFARRAAGAFVWVVGDAAKRGLKQIGVTAHGGVIMAIMYSFSHSD
                     QTYYDWYVQNCGGFRLVIDESAWAEGKPGFVSYERFGEAGDSQAMLGGESKA"
     gene            complement(967..1311)
                     /locus_tag="PLR57_05735"
     CDS             complement(967..1311)
                     /locus_tag="PLR57_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HQQ40994.1"
                     /translation="MILVIGARVSGKLEYVKSLGYSDADIANGVLDERKVVYNLQNVV
                     FRDPENAPNLLDELLQKEVVVCDEVGSGVIPLERQERLAREATGRLCIQLAQRAKCVV
                     RLVCGLATVIKE"
     gene            complement(1308..>1962)
                     /locus_tag="PLR57_05740"
     CDS             complement(1308..>1962)
                     /locus_tag="PLR57_05740"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HQQ40995.1"
                     /translation="GALIGALLCGWAWLCDALSIGKILFAAGMTLLPLLVSGGIHLDG
                     FCDTVDALSSHATQERKREILKDPHAGAFAVIGVCAYLLAVFALYTELPNEKNAVLLA
                     CLIPVLSRGAGALASFGTPSDGAGLLHTMRSSADRRAVVWILAVWCALGVSGLLLLSP
                     LTGGVMALAGVLCTVLTVRMAKRQFGGMSGDLAGFCIQVCELAMLCGLIFTQKAVLL"
BASE COUNT          407 a          627 c          534 g          394 t
ORIGIN      
        1 cctgtatcag cgccgcttcg ttgccgcacc agaaatcgag ccagtacgga aagtagccca
       61 tgtggatgcc gaacacgcgc tctttgggaa cgatgtttcg ctcgtcctgc tcacaggcca
      121 gcagttccac gccgtcgaaa ccttccatca gcgcgagcag ttccgcgcgg gttgaaaagc
      181 gctccatgtc gttcgggctg gtcgtgaggt tgaacagtgt tttcatacta tgcttttctt
      241 tgcgcgggag cgattgtcag attgaacagc gttttcaagt taaggtccct ttcaaatgcc
      301 atgagctgag aggcattgct tacgctttgc tttctccgcc gagcatggcc tgactgtccc
      361 ccgcctcgcc gaagcgctcg taggagacaa atcccggctt gccttccgcc caggctgact
      421 cgtcgatcac gagccggaag ccgccgcagt tctgcacgta ccaatcgtag tatgtttgat
      481 ccgaatgcga aaagctgtac ataatcgcca tgatgacccc gccgtgcgcg gtcacaccga
      541 tctgctttaa gccccgcttt gccgcatccc cgacaaccca gacgaacgct cccgccgctc
      601 ggcgcgcaaa ctgaggcacg ctttcgccgt tggggcaggc ctccatgccg ttgctttcga
      661 cccaggcgcg gtaagcggaa tcgtgctcca tctcatcggc ggttctgcct tcaaaatcgc
      721 caaagttcat ttcgcgaagg tcgttcacaa cgatctgctc ggcgttggga aagcagattc
      781 ccgccgtctg ccgcgcgcgc aggagcggac tgacgtacac atgcgtaaac gatgcatgct
      841 tttgtgccga atttgcctcc cgcaggcctt cctcgcaaag gggatcgtcc gtgcgcccgt
      901 tgtagcggtt gtcgaggttc gcagctgttt tgccgtgcct taagaggatg atttccatat
      961 tgtaagttac tccttgatga ccgtcgctaa gccgcagacg agccggacga cgcactttgc
     1021 gcgctgtgcc agctgaatgc agagcctgcc cgtcgcttcc cgcgcgaggc gctcctgccg
     1081 ttcgagaggg atgaccccgc tgccgacttc gtcgcaaacg acgacttctt tttgcagcag
     1141 ttcgtccagc aggttcggcg cgttttcggg atcgcgaaat acgacgtttt gtagattata
     1201 cactaccttt cgctcgtcga gcacgccgtt tgcgatatcc gcatcggaat atcccaaaga
     1261 ttttacatac tcaagctttc cggaaacgcg cgcgccgatc accagaatca taacaaaacc
     1321 gccttttgcg taaaaatcaa cccgcagagc atagcgagtt cgcagacctg aatgcagaac
     1381 cccgcgaggt cgccgctcat gccgccgaac tgacgcttcg ccatgcgtac ggtcagaact
     1441 gtgcagagaa cgccagccag cgccatcaca ccgcccgtga gcggagagag cagcagtaac
     1501 cccgaaacgc ccagcgcgca ccacacggcg agtatccaca ccaccgcgcg ccggtctgcc
     1561 gaagagcgca tcgtatgcag cagccccgcg ccgtccgacg gggtgccaaa gctcgccagc
     1621 gcgccggctc ccctgctcag caccggaatc aggcacgcaa gcagaaccgc gtttttctca
     1681 ttcgggagtt ccgtatagag cgcaaagacc gctaaaagat atgcgcaaac gccgatcacc
     1741 gcgaacgcgc ccgcgtgcgg atccttcagg atttcgcgtt tgcgttcttg cgtggcatgg
     1801 ctggaaagcg cgtccaccgt gtcgcaaaag ccgtccagat ggattccgcc ggaaacgagc
     1861 agcggcaaga gcgtcatgcc agccgcgaac aggattttac caattgacag cgcgtcgcag
     1921 agccacgccc agccacagag aagcgcgcca atcagcgcgc cc
//
LOCUS       DAOMGY010000336         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_66654_length_1961_cov_3.376705, whole genome shotgun sequence.
ACCESSION   DAOMGY010000336 DAOMGY010000000
VERSION     DAOMGY010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66654_length_1961_cov_3.376705"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="PLR57_05745"
     CDS             complement(<1..1135)
                     /locus_tag="PLR57_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQQ40996.1"
                     /translation="MMHDVQMGRQTRKSFSRIREVLDMPDLIEIQKNSYYRFLREDLK
                     EVFDDISPITDYSGKLVLEFVDYRIDLDSPKYSMEESKERDVNYAAPLRVTVRLINKE
                     TGEVKQQEVFMGDFPLMTPQGTFIINGAERVIVSQLVRSPGVYFSDSFDKVGKQLFAA
                     QVIPNRGAWLEYETDSNGVLYVKIDRQRKVHITALVRALGYGANSEIIELLGEDERLS
                     ATLAKDPTHSEAEGLIEIYKRQRPGEPPTEESARTLLNSLFFDKRYDLARVGRYKFNK
                     KLAIRARIRNHPAADNIIHPGTGEILCEAGQVIDRELAGTIQNAGVNEVWLQIGDSRV
                     KVVGNNFVRANEWLEFPPIEAGINEDVHLPTLKALLKEGEGLPE"
     gene            1520..>1961
                     /locus_tag="PLR57_05750"
     CDS             1520..>1961
                     /locus_tag="PLR57_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HQQ40997.1"
                     /translation="MKNRDDALAVLRQYNESEALIRHAYAVEGVMRHFAEKLGEDAEY
                     WGLVGLLHDVDYEKWPEEHLKVAPRLLAEAGFDADFIRAVEAHGWGLCSNVKPEKKME
                     IVLYTIDELTGLITAAAYMRPSRSVMDMEVSSVKKKFKDKGFAAG"
BASE COUNT          423 a          505 c          564 g          469 t
ORIGIN      
        1 cctcggggag gccttcgcct tccttgagca gggctttgag cgtcggcagg tggacgtcct
       61 cgttgatgcc cgcttcgatg ggcgggaatt cgagccattc gttggcgcgc acaaagttgt
      121 tgccaacaac cttgacgcgg ctgtcgccaa tctgcagcca gacttcgttg acgcccgcgt
      181 tctgaatcgt gcccgcgagt tcgcggtcga tgacctgccc cgcttcgcag aggatttctc
      241 ccgtgccggg gtggatgatg ttgtccgccg ccggatggtt gcggatgcgc gcgcgaatgg
      301 cgagcttctt attgaactta tagcggccta cgcgcgcgag gtcataacgc ttgtcaaaga
      361 acagcgaatt gagcagcgtt cttgcgcttt cttccgtcgg cggttcgccg gggcgctggc
      421 gcttgtagat ttcgatgagg ccttccgcct cggagtgcgt cgggtccttg gcgagcgttg
      481 ccgaaaggcg ctcgtcttcg ccgaggagct cgataatctc gctgttggca ccgtagccaa
      541 gcgcgcgaac cagcgccgtg atatgcacct tgcgctgacg gtcgatcttg acgtagagga
      601 cgccgttgga gtcggtttcg tactccagcc acgcgccgcg gttcggaatg acctgcgctg
      661 cgaagagctg tttgccgact ttgtcgaaac tgtcggaaaa atatacgccg ggcgaacgaa
      721 ccagctggga gacgataaca cgctccgcac cgttgatgat aaacgtgccc tgcggcgtca
      781 tgagcgggaa atcgcccata aagacttcct gctgcttgac ttctccggtt tccttattga
      841 ttaaacggac ggtcacgcga agcggcgcgg cgtagtttac gtcgcgctcc ttgctctctt
      901 ccatagagta tttggggctg tccaggtcga tacggtaatc cacaaactcc agtacgagtt
      961 tgccggaata gtcggtaatc ggggaaatat cgtcgaaaac ttctttgaga tcctccctca
     1021 gaaaacgata gtacgaattc ttttgaatct cgatgagatc cggcatgtcg agaacttctc
     1081 gaatcctaga aaagctcttt cgcgtctgtc tgcccatttg aacgtcatgc atcatcgcgt
     1141 actccccttg ccgacgcggg ttgtcgcccc gcgccatttc actgattttc tcgcgcttct
     1201 tcgttgcggt tccggcttat cgttcttacc cggatgtgag gctccatttt ctgtcttttt
     1261 gtgcaaaaat tgcacgcaag agcatatttt ggggcatact aatacatcct agggatcata
     1321 agtcaattgt gtactatatc atccgctttg tcacgtgtca agaaaatatt ctaaattata
     1381 ttgctgattt tcagcgtttt gttgtatgat gttgagaaat ggcgacattt ttgaaaaata
     1441 attcgtaatt tctaaaaata ccggccggaa gcgatgattt tatcacaaaa tccgacagaa
     1501 agcgagcgga aaccaaaaaa tgaagaacag agacgacgcg ctagccgttt taaggcagta
     1561 taacgagagt gaagcgctga ttcgccatgc ttacgccgtg gaaggcgtga tgcggcattt
     1621 tgcggagaag ttgggcgaag acgcggaata ctggggtctg gtcggcttgc tgcacgacgt
     1681 ggattacgaa aagtggccgg aagagcacct caaggtagcc ccgcgcctgc tcgccgaagc
     1741 cggattcgac gcggacttta tccgcgcggt ggaagcgcac ggttgggggc tttgcagcaa
     1801 cgtgaagccc gaaaagaaga tggagatcgt gctctacaca atcgacgagc tcacggggct
     1861 catcaccgcg gcggcgtata tgcgcccgag ccgcagcgtg atggacatgg aagtaagcag
     1921 cgtaaagaaa aagttcaagg acaagggctt tgccgcgggc g
//
LOCUS       DAOMGY010000337         1958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_66805_length_1958_cov_4.232790, whole genome shotgun sequence.
ACCESSION   DAOMGY010000337 DAOMGY010000000
VERSION     DAOMGY010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66805_length_1958_cov_4.232790"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="PLR57_05755"
     CDS             <1..986
                     /locus_tag="PLR57_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HQQ40998.1"
                     /translation="GSANRGVEGIMRQIAEHNLPTRRMSFCTDDKHLADIREEGHINY
                     IVRRAVANGISPMHAIQMATINTANTYGIRHYGAVAPGYRADLVLFDNLTDFNPQFVI
                     TDGKLFEPSVDTHIKPDPKIYNSVHLAPRKPDIYRLPIKGKARIIRLIPKELITALDE
                     EEVYTENGCFVPKNGLLKLAVLERHHASGRVGLGILRGFQIKDGAIATTVGHDSHNLV
                     VVGDNDADMYAAVTALEECGGGYVVVRNGEVLAKLVLSVAGLMSDAPLENILKHQHEL
                     LAAAATLGIHTDSDPFITLSFIALPVIPDVRLTDMGLFDVTKMEFFEKENG"
     gene            1346..>1958
                     /locus_tag="PLR57_05760"
     CDS             1346..>1958
                     /locus_tag="PLR57_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070123.1"
                     /note="molybdenum cofactor-binding protein; participates
                     in purine salvage; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HQQ40999.1"
                     /translation="MVVGKSVKRVDAIDKVTGRAKYTDDLADKSALIVKIYHSTIAHG
                     MVTKVDTSAAEAIPGVVKVLTCFDVPNLPFPTAGHPWSIDPHHQDVADRLLLNKHVRY
                     YGDDVAVVIAEDEVAASQGVRALKVEYEEYPFVLDQQEAMKDGAPQLHERFANNILAH
                     TGYEEGKFDEAIQEPGLVKIEGWYETPVVQHCHIENHICYAEEQ"
BASE COUNT          444 a          596 c          539 g          379 t
ORIGIN      
        1 tcggcagcgc caaccgcggc gtggaaggca ttatgcggca gattgccgag cacaacctgc
       61 ccacgcggcg gatgtccttc tgcacggacg acaagcacct tgccgatatc cgcgaggaag
      121 gccacatcaa ctatatcgtc cgccgtgctg tggcaaacgg catctccccg atgcacgcca
      181 tccagatggc gaccatcaac accgctaata cctacggaat ccgccactac ggcgcggtcg
      241 cgcccgggta ccgagcggat ctcgtgctgt tcgacaacct gaccgacttc aacccgcagt
      301 ttgtgattac cgacggtaag ctctttgagc cgagcgtgga tacgcatatc aagcccgacc
      361 cgaagatcta caacagcgtc catcttgctc cccgcaagcc ggatatctac cgcctgccca
      421 tcaaaggcaa ggcgcggatt atccgcctga ttcccaagga gctcatcacc gcgctggacg
      481 aagaggaagt ctacactgaa aacggctgtt tcgtaccgaa gaacgggctg ctcaagctcg
      541 ccgtgctgga gcggcaccat gcctccggac gcgtcggcct cggcattctg cgcgggttcc
      601 aaatcaagga cggcgcaatt gccaccacgg tcgggcacga ttcgcataac ctcgttgtcg
      661 tcggcgacaa cgacgcggat atgtatgccg cggtcacagc gctggaagag tgcggcggcg
      721 gatacgttgt tgtccgcaac ggcgaagtac tggcaaagct cgtcctctcg gttgccggtt
      781 taatgagcga cgcgccgctg gaaaacatcc tcaagcatca gcacgagctg ctcgcagccg
      841 cagcgacgct cggcatccat acggacagcg acccgttcat cacgctctcg ttcatcgcgc
      901 tgccggtgat tccggacgtg cgcctgaccg acatgggtct gttcgatgta acgaagatgg
      961 agttcttcga aaaagagaac ggctgatcgc cccgcttggc gcaataaacg ccgggcgttc
     1021 agcgtcctgc acagcctgtt tcgtggctat tccttcccgc gcgttgaaaa caaaccgctt
     1081 atcccggtca acagcggtat tctatcaaca taaatcacca atatgttgat cggcgcgtga
     1141 tatttcggtg gacaattttg aacgaatccc ctattccgct tataacgtgc taggatagaa
     1201 atgggtgatt ttacgcgctt tttgcggaac ttcgcccgtt tacgacgctc ccgccgcccg
     1261 ccggacgacc gcacgcccgg ctgagcgcaa ggatatttac accagtttta cgcattacgt
     1321 ttgatacagg ttcaggaggg gtactatggt cgttggaaaa agcgtcaagc gcgttgacgc
     1381 aatcgacaag gtgacgggcc gcgcaaaata cacggacgat cttgccgaca agagcgcgct
     1441 catcgtaaaa atctatcact ccaccatcgc ccacggcatg gtaacaaagg tggatacatc
     1501 cgccgcggaa gcgattcccg gcgtggtgaa agttctcacg tgcttcgacg tgccgaacct
     1561 gccgttcccg acggcggggc atccctggag catcgaccct caccatcagg acgtagccga
     1621 ccggctgctg cttaacaagc acgtccgtta ttatggcgac gacgttgccg tggtcattgc
     1681 cgaggacgaa gttgccgctt ctcagggcgt ccgcgcgctg aaggtggaat acgaagagta
     1741 tccctttgtg ctcgaccagc aggaagccat gaaggacggc gcgccgcagc tgcacgagcg
     1801 tttcgccaac aacattctcg cccacacggg ctatgaggaa ggcaagttcg acgaagcgat
     1861 tcaggaaccc ggccttgtca aaatcgaagg ctggtacgaa acgcctgtcg ttcagcactg
     1921 ccatatcgaa aaccacatct gctatgccga agaacaga
//
LOCUS       DAOMGY010000338         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_66865_length_1957_cov_3.783386, whole genome shotgun sequence.
ACCESSION   DAOMGY010000338 DAOMGY010000000
VERSION     DAOMGY010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66865_length_1957_cov_3.783386"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PLR57_05765"
     CDS             complement(<1..685)
                     /locus_tag="PLR57_05765"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HQQ41000.1"
                     /translation="MDSAQNNRICRLKRRLKIALAALAVLALCSGCSVAETLLDVTKS
                     PEVTPSDENLVVIADTKQPDTTDAPADERAEETSEGEAEETPAPTETPAPSTSFTSGR
                     ELPEGFVYRPVLAVIDNAAQSRPQTALMLADVVYEFPLDRTDHTTRYLAVFSDVIPER
                     VGPIRDSRAYLAETAREWGGLYLSAGDPADLREGYPLLSDAGLRFRAENSGTAADYFY
                     TDKTVTAIAE"
     gene            complement(678..1883)
                     /locus_tag="PLR57_05770"
     CDS             complement(678..1883)
                     /locus_tag="PLR57_05770"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HQQ41001.1"
                     /translation="MFKQHILLSGLIKRIVLFALTIGMLLALCGCSGFLPLAATPTIE
                     PTATVEPTPTQEPTPTPLPTPTPTPAPQNRSQTSGKVVPEGTPSKPFIMSIDNANGAK
                     PQTSLMQADIVYEFLVEQAITRFQAVFNDTYPIYAGPLRSTRYYIIDMVQEWDCMYLH
                     EGYVVLPKPYRRYPKNLITLYLPGGDFSGYSTNYSDYKGALDDLETKSGYKFRAPESG
                     RGTVHALYYRIAALVNDFYGDHEAKQCERFRFMENVSYEYGTPFTTVSLSYGYENNPD
                     WIQFTYDSEANRLFRSESGEPFMTRTLSEHGDTYTTEQMNVQNLIVQYCNYGTVKDDK
                     KHRRTCEMTGSGKCDYFINGQHVTGTWSRPTADDYTSYYLDDGSLVILEPGNTWIAVH
                     PSDTPVTIG"
BASE COUNT          366 a          543 c          619 g          429 t
ORIGIN      
        1 gttccgcgat agcggtcacg gtcttgtcgg tatagaaata gtccgccgcg gttccgctgt
       61 tttcagcgcg gaagcgaagc cctgcgtcgc tcagcaacgg gtaaccttcc cgcaaatcgg
      121 ccgggtcgcc cgcggaaaga taaagcccgc cccattcccg cgcggtttcg gccaggtacg
      181 cgcgtgaatc gcggatgggg ccgacgcgct cgggaatcac gtccgaaaac accgccagat
      241 accgcgtggt atggtcggtg cggtcgagcg gaaactcgta aaccacgtcc gcgagcatca
      301 gcgccgtctg cgggcggctc tgcgccgcgt tgtcgattac cgcgagcaca ggccggtaga
      361 caaacccttc cggcagttcg cgaccggatg taaacgaagt cgagggcgct ggcgtttcgg
      421 taggcgcggg cgtttcctca gcttcgcctt cggaagtttc ttccgcccgt tcatcggcag
      481 gcgcgtcggt agtatcaggc tgctttgtat ctgcaatgac aacgaggttc tcgtcgcttg
      541 gcgttacttc cggcgatttg gttacgtcca gcagcgtttc ggcaacggaa cagccggaac
      601 agagcgcaag cacggcaagc gccgccagcg caatcttcag ccgccgtttc agccggcata
      661 tgcggttgtt ttgcgcgcta tccaatggtc accggggtgt ccgacgggtg caccgcgatc
      721 caggtattgc ccggttcgag gatgacgagc gatccgtcgt ccagatagta ggaagtatag
      781 tcatccgcgg tcgggcgcga ccaggtgccg gttacgtgct ggccgttgat aaagtagtcg
      841 catttgcccg agccggtcat ttcgcaggtg cggcggtgct tcttgtcgtc cttcacggtg
      901 ccgtagttgc agtactgtac aatgagattc tgaacgttca tctgctcggt tgtatatgtg
      961 tccccgtgtt ccgagagcgt ccgcgtcata aacggctctc cgctttcgct gcggaagagg
     1021 cggttcgcct ccgaatcgta ggtaaactga atccaatcgg ggttgttttc gtacccgtag
     1081 gaaagcgaaa cggtggtaaa cggcgtaccg tactcataag agacattctc cataaagcga
     1141 aaccgttcgc attgtttggc ttcgtggtcg ccgtaaaaat cgttgaccag tgcggcgatg
     1201 cggtaataca gcgcgtggac ggtgccgcgg ccggattcgg gcgcgcggaa tttgtagccg
     1261 ctcttggtct ccaaatcgtc gagcgcgcct ttataatcgc tgtagttggt ggaatagccc
     1321 gaaaagtcgc cgccgggaag gtagagcgtg atgaggttct tcggataccg gcgatagggc
     1381 tttggcagga cgacataccc ttcgtgcagg tacatgcagt cccattcctg caccatgtcg
     1441 ataatatagt accgcgtcga gcgcaagggc cccgcgtaaa tcggataggt gtcgttgaac
     1501 accgcctgaa agcgcgtaat ggcctgctcc acgaggaact cgtatacgat atccgcctgc
     1561 atgagcgagg tctgcggttt cgctccgttg gcgttgtcga tggacatgat gaacggcttc
     1621 gacggcgtgc cttccggaac gaccttgccg gacgtctgcg agcggttctg cggcgcgggc
     1681 gtgggcgtcg gggttggaag cggcgtcggc gtcggttcct gcgtgggcgt cggttcaacc
     1741 gttgcggtcg gctcgatcgt cggcgttgcg gcgagcggca gaaaacccga acagccgcaa
     1801 agcgcaagca gcattccaat ggtcagcgca aacaagacaa tgcgttttat gagaccggaa
     1861 agcaatatat gctgcttaaa catatcagcg gtctcccttg cgtttgccgt cggtttcttc
     1921 gagctcgtgc tgtttcctgc ggtgaacggc ggttacg
//
LOCUS       DAOMGY010000339         1954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_67043_length_1954_cov_2.496577, whole genome shotgun sequence.
ACCESSION   DAOMGY010000339 DAOMGY010000000
VERSION     DAOMGY010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67043_length_1954_cov_2.496577"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLR57_05775"
     CDS             complement(<1..418)
                     /locus_tag="PLR57_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41002.1"
                     /translation="MNEAVLTGHYQKRGFSQEVTLQAVQFVYALETHLQAAGSSLETA
                     SVNEIRAYIRLLMKQEGFSLEHLLALARYFYLTGRNEIYIYFTSLLGGEGVVESISDR
                     LAESVGASEAKRILEGLKHPPLGSEPADFPAFTKALM"
     gene            721..1770
                     /locus_tag="PLR57_05780"
     CDS             721..1770
                     /locus_tag="PLR57_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQQ41003.1"
                     /translation="MKRVYIITGIGGHLGNTVARELLAAGETVRGFALPNEDVTMLYG
                     DAVSIVRGDVRDKASLEPLFFGLTEEDETYVIHCAGIVSVASKYDQRVVDVNVRGTEN
                     VVALCKEHNVKKLVYISSVHALPELPHGNVIHEVDGYQADAVIGLYAKTKAAATQIVL
                     DAARSGLDATVIQPSGIIGPNDYGHGHLTQMVINYLNGSLTACVEGGYDFVDVRDVAD
                     GVIAATEKGKKGECYILSNRYIPVRELLGTLSRVTGGKKITTVLPLWFAKLTAPLAEL
                     YYKIRHESPLFTRYTLYTLTSNARFTYEKAKNDLGYKPRDLSTTIKDTVSFLTKERKV
                     NVRKRRPTRLAPTMG"
BASE COUNT          462 a          533 c          549 g          410 t
ORIGIN      
        1 ccatcagagc cttggtgaaa gcgggaaagt ccgccggttc gctgccgagc ggcggatgct
       61 tcagaccttc gagaatgcgt ttcgcctcgg atgcgccaac ggattcggcc agacgatccg
      121 aaatactttc cacaacgcct tctccgccga gcagggacgt aaagtagatg tagatttcgt
      181 ttctgccggt cagataaaag tatcgcgcca gcgcgagcaa atgctcaagc gaaaagccct
      241 cctgcttcat caaaagccgg atgtatgcgc ggatttcgtt caccgaagcg gtttccagcg
      301 aggaaccggc cgcctgcaga tgcgtttcca gcgcgtaaac gaactgtacg gcctgcagcg
      361 taacttcttg ggagaagccc cgtttttgat agtgcccggt cagaacggct tcgttcatgc
      421 gataacctcc cgaaagtttg attgcggaat atgcgtgttg atatgcgtta cttccatttt
      481 accggcggcg cgcgtcatgc gcaagtgttt gtctaaaagt ttcgattgat gtgagaacgg
      541 ttctcgttat ctcgtttgag gaagaaatcg caggcaagtt gaggttcggt tgaggttggg
      601 ttgaactccg gttgaagttc ataaagaaaa cttaaatatg tcgacagatt gtcagttttt
      661 cgtaataact gcgtaacatt caccccgtat catggaacca tgaaagtgag gcattcgtct
      721 atgaaacgcg tttatattat tacaggaatc ggcgggcatc tcggcaacac ggtcgcgcgg
      781 gaattacttg cagccgggga aacggtgcgc gggttcgcgc tgcccaacga ggacgtcacc
      841 atgctctatg gcgacgcggt gtccattgta agaggcgacg tgcgcgataa agcctccctt
      901 gaaccgctct ttttcgggct gacggaagag gatgaaacgt atgtcatcca ctgcgcgggc
      961 atcgtctccg tggcctctaa atacgaccag cgcgtcgtgg acgtgaatgt ccgcggcacg
     1021 gaaaacgtcg ttgcgctctg caaggaacac aacgtaaaaa agcttgtcta catcagctcc
     1081 gttcacgcgc tgccggaact gccgcatggc aacgtgatcc acgaagtgga cggctatcag
     1141 gcggacgccg tgatcggcct ttacgccaag acgaaagccg ccgcaacaca gatcgtgctc
     1201 gacgccgcga gaagcgggct cgacgcgacc gtgattcagc cttccggcat catcggcccg
     1261 aacgattacg gtcacgggca tctgacccag atggtcatca attacctcaa cggcagcctc
     1321 acggcctgcg tcgagggcgg atatgacttt gtggacgtgc gggacgtcgc cgacggcgta
     1381 atcgccgcga ccgaaaaggg caagaagggc gagtgctaca tcctctcgaa ccgctatatc
     1441 cccgtgcgcg aactgctcgg cacgctttcc cgcgtgaccg gaggaaagaa gattacaact
     1501 gttctgccgc tgtggtttgc aaagctgacc gcaccgcttg cggaactgta ctacaagatt
     1561 cgccatgagt cgccgctgtt tacgcgctac acgctctata cgctgaccag caacgcgcgc
     1621 tttacctacg aaaaggcgaa gaacgacctc ggctacaagc cgcgcgatct cagcacgacc
     1681 atcaaggaca cggtatcgtt cttaaccaag gagcggaagg tcaacgtccg caaacgccgc
     1741 ccgacgcgcc ttgcgccgac gatgggctga aaaacaagat taaaaaaccg ttccggttca
     1801 aaccgggacg gttttttatt gtttaatcga tcggagagaa tgtaaccgct actccgcgga
     1861 ttcctccttg aggcgctcgt aaacaacgta aggccggtcg agatggtagc caagcttctc
     1921 ggcaagggag acggagcgca aatcgtaagc gtcc
//
LOCUS       DAOMGY010000340         1953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_67080_length_1953_cov_3.242887, whole genome shotgun sequence.
ACCESSION   DAOMGY010000340 DAOMGY010000000
VERSION     DAOMGY010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67080_length_1953_cov_3.242887"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1322
                     /locus_tag="PLR57_05785"
     CDS             156..1322
                     /locus_tag="PLR57_05785"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HQQ41004.1"
                     /translation="MQIVWNVLIVLVMALGPRLMVSLSKRVKVLGMLGSVFLCYAGGF
                     LLSLVLPDTSIAMDISEILIPIAIPLILFSADLTSIKRLAKPMLNSFLLVCAAVVGVA
                     VASYLLYRNLLPEAYKYAGMIVGLYTGGTPNLMAIGAALSVNDSHIVLANASDVVVGG
                     VYFLLLISIMPKLARKFLKPFDAGAQTAAPEDATYTEHLEKNFVPEKEPLSLKSLLRR
                     VPIVLLGVLSLAIAAGLSLLITGGLNVVVIMLVVTSCGVGFSFIKPVRNAPGSYTAGQ
                     YLILVFSFGIGLSFRFSAIKTESLIFLAMFATAQFGAILLHLILAKIFKIDADTALIT
                     STAGIYGPAFIAPAADALKNREVVLPGLICGIFGYAIGNYLGIGVALLLKALRG"
     gene            1403..>1953
                     /locus_tag="PLR57_05790"
     CDS             1403..>1953
                     /locus_tag="PLR57_05790"
                     /inference="COORDINATES: protein motif:HMM:NF021988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HQQ41005.1"
                     /translation="MQSIRDQLNSGALDGALKDLYGAEALEEQRARFLMLEREFTAQF
                     GESENEALFSAPGRSEIGGNHTDHQLGRVLAAAVTVDVCAVAAPRKDGVVRIHSLGFP
                     PLSVSLDQLAPNAGEQNASEALVRGIAERFTQQGRNVGGFDAVTVSDVPKGSGLSSSA
                     AFSILVCTIFSELYNKGNIPPVE"
BASE COUNT          371 a          548 c          578 g          456 t
ORIGIN      
        1 gccggcggtt ttccgtcggc gtttttttgt ataaaacagt atatttgatg ctgcttatcc
       61 gctctgacgg tttgcccgca aaagcggggc gtgatataat cgttcaggcg tattccgctt
      121 gcgtgcggag aaaccgaacg ccgcgggagg gaaacatgca aatcgtatgg aatgtgctca
      181 ttgtactcgt catggcgctg gggccgcggc ttatggtttc gctttccaag cgcgtgaagg
      241 tgctgggtat gctcggatcg gtgtttctct gctatgcggg cggattttta ctgagccttg
      301 ttctgccgga cacctcgatt gcaatggata tttcggaaat actgattccc attgcgatcc
      361 cgctgatcct gttttccgcc gatctcacaa gtataaaaag gcttgccaag ccgatgctga
      421 actcgtttct gctggtttgt gccgcggtcg ttggcgttgc ggtcgcctca tatctcctct
      481 accgcaatct tttgccggaa gcgtataaat acgctggcat gatcgttggt ctctacacgg
      541 gcggcacgcc gaacctcatg gccattggag ccgcattgtc ggtgaacgat tctcatatcg
      601 tactggccaa cgcttcggat gttgtggtcg gcggcgtata cttcctgctg ctcatctcaa
      661 ttatgccgaa actggcaaga aagtttttaa agccgttcga tgcgggcgct cagacggcag
      721 cgccggagga tgcaacttac accgaacacc tggaaaagaa ctttgtgccg gaaaaagaac
      781 cgctttcgct caagtcgctg ctgcggcggg tgccgatcgt actgctcggc gtgctctcgc
      841 tggcgatcgc ggccgggctg tcgctgctga ttacgggagg gctgaacgtt gttgtgatca
      901 tgctcgtggt cacgagctgc ggcgttgggt tttcgtttat caagcctgtc agaaacgcgc
      961 ccggcagcta cacggcgggg cagtacctga ttctcgtgtt ttcgttcggc atcggcttgt
     1021 cgttccgctt cagcgcaatt aaaaccgagt cgcttatctt tctggcaatg tttgccaccg
     1081 cgcagttcgg cgcgatttta ctgcacctga tcctggctaa gatcttcaaa atcgacgcgg
     1141 acaccgcgct gattacgtct acggcgggca tctacggacc cgcgtttatc gcaccggccg
     1201 ccgacgctct caagaaccgc gaagtggttc tgccggggct catctgcggc attttcggct
     1261 acgccatagg caactacctc ggcatcgggg tcgcgctgct gctcaaggcg ctccgcggct
     1321 gagacagcgc ggcgtttcct gctgaaaaaa ccggattgat atagaaaaat gaactactta
     1381 tataacagcg gaggggattg ctatgcagtc aattcgtgac caactcaaca gcggcgcgct
     1441 ggacggcgca ctgaaagacc tttacggagc ggaagcgttg gaagagcagc gcgcgcggtt
     1501 tctcatgctg gagcgcgagt ttaccgcgca gttcggcgaa agcgaaaacg aagcgctctt
     1561 ctccgcgccc gggcgcagcg aaatcggcgg caatcacacc gaccatcagc ttgggcgagt
     1621 gcttgcggcg gctgtgacgg tggacgtctg cgcggtggcc gctccccgca aggacggcgt
     1681 cgtgcgcatt cactcgctgg gattcccgcc gctctccgtc agcctcgatc agctcgcgcc
     1741 caacgccggc gaacagaacg ccagcgaagc gctggtgcgc ggcattgccg agcggttcac
     1801 acagcagggg cgaaacgtgg gcggctttga cgcggtcacg gtttcggacg ttccaaaggg
     1861 ttccgggctt tcctccagcg cggcgttcag catcctcgtg tgcaccattt tttccgaact
     1921 gtacaacaaa ggcaatatcc cgcccgtgga aca
//
LOCUS       DAOMGY010000341         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_67126_length_1952_cov_3.404323, whole genome shotgun sequence.
ACCESSION   DAOMGY010000341 DAOMGY010000000
VERSION     DAOMGY010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67126_length_1952_cov_3.404323"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /gene="dnaA"
                     /locus_tag="PLR57_05795"
     CDS             complement(<1..1274)
                     /gene="dnaA"
                     /locus_tag="PLR57_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQQ41006.1"
                     /translation="MDQLQSIWAAARENIRPQMTGLSFHAWIDVIVPLALHQNTLVLE
                     VPAPDIQKTLEDFYFESLFNAVKAANDTISDVKIVLPEDRDIYINPVSSEPINVFALN
                     PKYTFETFVVGGSNHFAHAAALAVAQNPAAAYNPLFIYGGVGLGKTHLMHAIGHSVKT
                     KDPSARVMYVTSETFTNELITAIQMDKRLEFRKRYRNVDVLLIDDVQFIAKKQSVQEE
                     FFNTFNTLHNANKQIIISSDRPPKEISSLEERLRSRFEWGLIADIQPPDVETRIAILR
                     NRARLDNLDVDDEIIQFIAEHVVSNIRELEGSLTRVVAYSQLRRRPLTLDLTIEALRD
                     LLPDVKKKEITPQIIKSTVAEYYSIPVESLLSERRDREIVLPRQIAMYLCHDMLGIPY
                     KRISTLFDKNDHTTAINACKRVEDMIESDHSFS"
     gene            complement(1294..1614)
                     /locus_tag="PLR57_05800"
     CDS             complement(1294..1614)
                     /locus_tag="PLR57_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41007.1"
                     /translation="MNAYRNAGDTALAFAIQLGNGFFFRFLCAVLGQNNAYLSVLSLH
                     KRKLYAFLSEAFVPVFTAFTGIFLAENACLPHSASLKRNLPGRNTAPEPCLENRPVFC
                     YDIG"
BASE COUNT          507 a          473 c          517 g          455 t
ORIGIN      
        1 gaaaaagaat gatccgattc gatcatatcc tcgacgcgct tgcaggcgtt gattgccgtc
       61 gtgtggtcgt tcttatcaaa aagcgtgctg atacgcttat aggggattcc cagcatgtcg
      121 tggcaaagat acatagcaat ctgcctcggg agcacgattt cacgatctct gcgttcggaa
      181 agaaggcttt cgaccggaat cgagtaatac tccgcaaccg tactttttat gatctgcggc
      241 gtaatctctt ttttcttgac atccggcagc agatcccgca gcgcttcgat ggtgagatcc
      301 agcgtcagag gccttctacg caactgcgaa taggcaacga cgcgggttaa agacccttcg
      361 agttcacgaa tgttgctgac tacgtgctcg gcaatgaact ggataatctc gtcgtccacg
      421 tccagattgt ccaggcgcgc gcgattgcgt aagattgcga ttcgggtttc gacgtccggc
      481 ggctgaatat ccgcaatcaa gccccactcg aaacgcgagc ggagacgttc ctccagactg
      541 gaaatctcct tgggcggacg gtcggaactg atgataatct gcttgttggc gttgtgcagc
      601 gtgttgaagg tgttgaagaa ctcctcctgc acggactgct ttttggcgat aaactgcacg
      661 tcgtcgatta agagcacgtc aacgttgcgg taacgcttgc gaaattccag ccgcttatcc
      721 atctggatgg cggtgatgag ctcgttggtg aacgtttcgc tggtgacgta catgacgcgc
      781 gcggagggat cctttgtttt gacggagtgc ccaatggcgt gcatgaggtg cgttttgccc
      841 aaaccgacgc cgccgtaaat gaagagcggg ttatatgccg ccgcggggtt ttgcgcaacc
      901 gcaagcgccg ccgcgtgcgc aaagtggttt gaaccgccga ccacgaaggt ttcaaatgtg
      961 tacttcgggt tcagtgcgaa cacgttgatc ggttccgaac tcacggggtt gatatagata
     1021 tcccggtctt ccggcaggac aattttcacg tccgaaatcg tgtcgttagc tgccttaacc
     1081 gcgttgaaaa ggctttcgaa atagaagtcc tcaagcgtct tttgaatatc cggcgcagga
     1141 acttccaaaa cgagagtatt ctgatggagc gccagaggaa cgatcacgtc gatccaggcg
     1201 tgaaacgaaa gccccgtcat ttgaggacgg atattttccc gcgctgccgc ccaaatcgat
     1261 tgtaattgat ccatgttcga cgtaccccct gtattaccca atatcatagc aaaaaacagg
     1321 ccggttttca aggcaaggtt ccggcgctgt atttcggccc ggcaggttcc gttttaatga
     1381 ggctgaatgc ggcaggcacg cattttcagc aagaaaaatc ccagtaaaag cggtgaaaac
     1441 cggaacaaac gcttctgata aaaaagcata aagttttcgt ttatgcagtg aaagaacaga
     1501 caaatatgcg ttattttggc ctaaaacagc gcacaaaaaa cgaaaaaaga agccgtttcc
     1561 aagctgaatt gcgaaggcaa gtgcagtatc gcctgcattc cggtacgcat tcatcaacag
     1621 tattccccat gttagcacta gaaaacacgc aaatcaacag taaaacgccc cacattttac
     1681 ctaaaacggg taaaaacggc ttgataagga ttaacaatct gtgacaaaca ggcggtttga
     1741 ttgcaaaacg gatattcaga ggttacaata gagtaagaat attggtatcg ggggaagatt
     1801 gcccgtggga gaatcgctgc gcagtcagtt attcatcaac tccgaaaagg tgggcgactt
     1861 cgtcgaaatc accctacccg cagccgggga ggatgaggac ggcgaaataa cgcgcatcca
     1921 ctatttggaa gcaggcgttg gcgaaccgct gc
//
LOCUS       DAOMGY010000342         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_67307_length_1949_cov_2.695354, whole genome shotgun sequence.
ACCESSION   DAOMGY010000342 DAOMGY010000000
VERSION     DAOMGY010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67307_length_1949_cov_2.695354"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..658
                     /locus_tag="PLR57_05805"
     CDS             11..658
                     /locus_tag="PLR57_05805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQQ41008.1"
                     /translation="MELRLETPQDYSEVEHITREAFWNKHVPGCDEHYLAHVLRSSAA
                     FVPELDFVAVHDGKVVGNIMYTKAVILCDDGTERNVLSFGPISVLPLMQGQGVGSMLI
                     RHTLEKAKELGYCAVLIYGDPGYYSRFGFVSAEQYGIGTSDDFYAEALQALELVPDAL
                     AKSAGKFFEDHAYEIDEAAAAAFDKQFLPKALRDDLPSQRHFKEMLKARKPRTIG"
     gene            763..1644
                     /locus_tag="PLR57_05810"
     CDS             763..1644
                     /locus_tag="PLR57_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HQQ41009.1"
                     /translation="MKLQYECLTCIIAQVLNVAKMLNFDEAHKEAAMRESLAYLSGAN
                     YEGCTPESMGELWELLLKHAETGDPYAVVKSLCNQEAMKMESHVRQAILNSGDPLGRA
                     LKFAVAGNLVDYGLEHPVSLEEQNRQVDEIAATPFVVDDSLELKEALRKAETLLYLCD
                     NAGEILFDKLLIEEIRREFPQIQATCVVKGTPVLNDVTMADALEVGLDQAARVIDNGD
                     GCPGTVLHRTSESFRREYEAADIVISKGQGNFESLTGQPKANIFFLFMAKCDAICQIV
                     GTSKLSMLCKKNEPHLN"
     gene            1798..>1949
                     /locus_tag="PLR57_05815"
     CDS             1798..>1949
                     /locus_tag="PLR57_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HQQ41010.1"
                     /translation="MATPVMMPRQGQSVESCIITEWKKKVGDAVQVGDILFSYETDKS
                     TFEEAA"
BASE COUNT          496 a          477 c          529 g          447 t
ORIGIN      
        1 gcgccaagat atcgaactcc ggctggaaac gccgcaggat tattctgaag tggagcacat
       61 tacgcgcgaa gcgttttgga ataaacatgt gcccggatgc gatgagcatt accttgcgca
      121 tgtactgcgt tccagcgccg cgtttgtacc ggaactggac tttgttgcgg tgcacgacgg
      181 caaagtggtt ggtaatatca tgtataccaa agcggtcatc ctctgcgacg acggcacgga
      241 aaggaatgta ctcagttttg gcccgatttc cgttttacct ttgatgcagg gacagggcgt
      301 aggatcgatg ctgattcgcc atacgctgga gaaagcaaaa gagctgggtt attgcgcggt
      361 gctcatctac ggcgatccgg gttattactc cagatttggc ttcgtgtccg ccgaacaata
      421 cggaatcggc acttcagacg atttttacgc ggaagctctg caggcgctgg aacttgtgcc
      481 ggacgcgctg gccaagagcg cagggaaatt tttcgaagac cacgcttacg aaatcgatga
      541 agcagcggcc gctgcgttcg acaaacagtt cctgccgaaa gccctgcggg acgatctgcc
      601 gtcccagcgt catttcaaag aaatgctcaa agcgagaaaa cccagaacga tcggttaagg
      661 aacacgcttt cagtatatag aagtattcaa acgacccgcg tattgccacg cgggtttgct
      721 ttatggttgc atttcgaatt ttcttccgtt agaatacccc ttatgaaact acaatatgaa
      781 tgcctgacct gcatcatcgc gcaggtgctc aacgttgcca aaatgctcaa tttcgacgaa
      841 gcgcacaagg aagcagccat gcgggaatcc ctcgcgtatt taagcggcgc caattacgaa
      901 ggctgtacgc cggaatccat gggcgaactt tgggagcttc tgctcaagca cgcggaaacg
      961 ggcgatcctt acgccgtggt caagtccctc tgcaatcagg aagccatgaa gatggaatcc
     1021 cacgtgcggc aggcgattct caattccggc gatccgctcg gacgtgcgct gaagttcgcc
     1081 gttgcgggaa atctggtgga ctacggtctg gaacatcctg tttcgctgga ggagcagaac
     1141 cgtcaggtgg acgaaatcgc cgccacgcct tttgtcgtgg acgacagcct cgaactgaaa
     1201 gaagccttgc gaaaagcgga aacgcttctg tacctttgcg acaacgcggg cgaaatcctg
     1261 tttgacaaac tcttgattga ggagattcgc cgtgagtttc cgcagattca ggcaacctgt
     1321 gtcgtaaagg gaacgcctgt gctcaacgac gtgactatgg cggacgcgct ggaagtcggg
     1381 ctggatcagg ctgcccgcgt catcgacaac ggcgacggct gccccggtac ggttttgcac
     1441 cgcacgagcg aatcgttccg cagggagtat gaggcggcgg acattgtgat ttcaaaaggg
     1501 caggggaatt ttgaaagcct gaccgggcag cccaaagcga atatcttctt tctgtttatg
     1561 gcaaaatgcg acgcgatctg ccagatcgta ggtaccagca agctcagcat gctttgcaag
     1621 aaaaacgagc cgcatttgaa ctgaaaacag atttatagat cgaatcttat gctgtggaac
     1681 cgcgcgaatt acattctgcg gttccgtttc ttttttcttg acagcatgct ataaatgtta
     1741 taatataaca tcataaagaa atattattac attcaattca ttctaaggag gctcactatg
     1801 gcaactccag tcatgatgcc gaggcagggg cagtccgttg aaagctgcat catcaccgaa
     1861 tggaaaaaga aagtcgggga tgcggttcaa gtcggcgata ttctgttttc ttacgaaacc
     1921 gataaatcca catttgaaga agcggcaaa
//
LOCUS       DAOMGY010000343         1943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_67592_length_1943_cov_3.839513, whole genome shotgun sequence.
ACCESSION   DAOMGY010000343 DAOMGY010000000
VERSION     DAOMGY010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67592_length_1943_cov_3.839513"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..357)
                     /locus_tag="PLR57_05820"
                     /pseudo
     CDS             complement(172..357)
                     /locus_tag="PLR57_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014281119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
     gene            complement(379..1473)
                     /gene="nusA"
                     /locus_tag="PLR57_05825"
     CDS             complement(379..1473)
                     /gene="nusA"
                     /locus_tag="PLR57_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HQQ41011.1"
                     /translation="MNTEFVEALNALEKERGISKDILIMAIEAALTSAYKRNYGSTAN
                     VRAEVDPTTGEFRIYASKTVVDTVENPLNEILLADAKRVNMLYEVGDVLEEEVKAKEF
                     SRIAAQTAKQVVVQRIREAERGVIYEEYVEKENEVLTAIVQRVEKGNVYVELGKTDGI
                     LTPNEMIPGETYENNDRIKVYVLEVRKDSKGPQVLVSRTHPGLVKRLFELEVPEIQTG
                     VVQIKSIAREAGFRTKVAVHSNDMQVDPVGACVGQRGARVERIVGELHNEKIDIIEWD
                     SDSAVYIAKALSPAKVLMVYINEEEKAAKVVVPDSQLSLAIGKEGQNARLAAKLTGWK
                     IDIKSQTQAEQVMDSIYEEDYPADEAESEE"
     gene            complement(1559..>1943)
                     /gene="rimP"
                     /locus_tag="PLR57_05830"
     CDS             complement(1559..>1943)
                     /gene="rimP"
                     /locus_tag="PLR57_05830"
                     /inference="COORDINATES: protein motif:HMM:NF036577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HQQ41012.1"
                     /translation="DVEYQKEYGSWVLTLYIDRAEGVSIDDCERVSKAVDPVLDEADP
                     IEQAYYLSVSSLGLDRPLKKDADYTRNLGKRIEIKLFAPMNGKKEFVGELVSFTDEQV
                     TVRQDGSELVFERKSIALARPELVF"
BASE COUNT          346 a          610 c          535 g          452 t
ORIGIN      
        1 cagcaagact cccgggctat cgaccagttc ggcctggcgc agcaggaacc gcgcagccgc
       61 accggccgga tcgcgcgcgc aaaggtgcag cagcgaaggc cgggccagcc gggcgacgag
      121 ctggacaaag ccaaacccgt tcatcgaggt ccgctcatgc ggccaatcgc ccagcgcgcg
      181 ttccagcgcg cgggacttga ttgcgttgtc gaggcacgcg gccttcaagc agagatacgc
      241 gccgcgtccg ttggcgcgcc cggtggggtc tatgaccacc gctccctcgg gcgtccgcac
      301 gacgcgcgtg agctcctttt tcggccgcat ctcacggcag gaaacgcaca tgcgcatggg
      361 aatcttcttt gccaaggctt actcctcgct ttcggcctcg tcggcgggat agtcctcttc
      421 gtaaatcgag tccatcacct gttccgcctg ggtctggctc ttgatgtcga tcttccagcc
      481 ggtcagcttg gcggcaagac gcgcgttctg gccttccttg ccgatggcga gcgaaagctg
      541 agaatccggc acgacgacct ttgccgcctt ttcttcttcg ttaatataca ccatgagcac
      601 ctttgccggg ctcagggctt tggcaatata caccgccgag tcggaatccc actcgatgat
      661 atcgatcttt tcgttatgca gttcgccgac gatgcgctcc acgcgcgcgc cgcgctggcc
      721 aacgcatgcg ccgaccgggt ccacctgcat gtcgttggaa tgcacggcaa ccttggtgcg
      781 gaagcccgct tcgcgcgcga tggacttaat ctgcacaacc cccgtctgaa tctccggcac
      841 ttccagttcg aagagccgct ttacgagacc ggggtgcgta cgggaaacga gaacctgcgg
      901 gcctttgctg tccttgcgga cttcgagcac atagaccttg atgcggtcgt tgttttcgta
      961 ggtttcgccg ggaatcattt cgttgggagt gaggatgccg tcggttttgc cgagttcaac
     1021 atacacattg cccttttcca cgcgctggac gatggcggtg agcacttcgt tttctttttc
     1081 tacgtattct tcgtagatta cgccgcgttc cgcctcgcgg atgcgctgca cgacgacctg
     1141 cttggccgtc tgcgcggcaa tgcgcgaaaa ctccttggct ttgacttcct cttcgagcac
     1201 gtcgcccact tcgtagagca tgttgacgcg cttggcgtct gcaagcaaaa tctcgttgag
     1261 cggattttcc accgtgtcca cgaccgtttt ggaagcataa atacggaatt cgcccgtggt
     1321 ggggtcaacc tccgcgcgta cgttcgccgt ggaaccgtag ttgcgcttat aggcggacgt
     1381 gagcgccgcc tcgattgcca taatcagaat atccttgctg attccgcgct ccttttccag
     1441 tgcgttcaat gcttcgacaa actctgtgtt catcttttct actccttcaa aagattcccg
     1501 ttttccggca cctgtgccga ggcactgcgg cgcgcgggta aaaaattcgt tctattcgtt
     1561 aaaacaccag ttccggccgc gccagcgcga tgctcttgcg ctcgaaaacc agttcgcttc
     1621 cgtcttgacg cacggttacc tgctcgtccg taaacgaaac cagctcgccg acgaattcct
     1681 ttttgccgtt catcggcgca aagagcttga tctcgatccg cttgccgaga ttgcgggtat
     1741 agtccgcatc ctttttaagc ggacgatcca gccccagcga cgaaacggag aggtaatacg
     1801 cctgctcaat cgggtccgct tcgtcgagca cgggatcgac ggccttgctc acccgctcgc
     1861 agtcgtcaat ggagacgcct tccgcacggt cgatatacag cgtcagcacc cagctgccgt
     1921 attctttttg gtactccacg tcg
//
LOCUS       DAOMGY010000344         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_67772_length_1940_cov_2.875862, whole genome shotgun sequence.
ACCESSION   DAOMGY010000344 DAOMGY010000000
VERSION     DAOMGY010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67772_length_1940_cov_2.875862"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLR57_05835"
     CDS             complement(<1..319)
                     /locus_tag="PLR57_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HQQ41013.1"
                     /translation="MEKKSSGIGFFEKYLTVWVILCMAAGVLIGQFLPGVPAFLGKFE
                     YANVSIPIAILIWLMIYPMMLKVDFQSIRNVGKNPKGLFVTWVTNWLIKPFTMFGIAW
                     LFFF"
     gene            complement(374..901)
                     /locus_tag="PLR57_05840"
     CDS             complement(374..901)
                     /locus_tag="PLR57_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005887206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HQQ41014.1"
                     /translation="MKNFLKRYGAFFLLLAAGVATGLFLPEIGKKSVAITLQNLKEML
                     SVLPPIFILLGLLDVWVDRATMMKFTGKGSGLKGVLIAFLLGSAAAGPLYAAFPVAGV
                     MLKKGSSLFNVFIFIGAWSTTKIPMLAFEGASMGLTFTLLRLGLSIIGILIIALITEK
                     SLSKAQQEEIYQINQ"
     gene            complement(898..1377)
                     /locus_tag="PLR57_05845"
     CDS             complement(898..1377)
                     /locus_tag="PLR57_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HQQ41015.1"
                     /translation="MFTYILYALAVIGLTVSFFKDRKKTKMALVKAWKAFENILPQLL
                     TILLIIGFALAVLSPETIQKLIGARSGVLGVLAAALIGSITLVPGFVAFPLAAALLKN
                     GAGYMQIAAFVSTLMMVGVITLPMELKTFGKRAAIVRNVSALGFSLIAAAVIGVVLK"
     gene            complement(1400..1753)
                     /locus_tag="PLR57_05850"
     CDS             complement(1400..1753)
                     /locus_tag="PLR57_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQQ41016.1"
                     /translation="MALFGKRKEKEETKSSCCCGGACDCESMEKAEQAKTEGASVKVL
                     GSGCPKCNALELATKTALAQLGMDTEIEHVTDYAKIASYGVMTTPALVIDGKVVSYGK
                     VLKTEEVVAILKKVR"
BASE COUNT          468 a          569 c          472 g          431 t
ORIGIN      
        1 cgaagaaaaa cagccacgca atgccgaaca tggtgaacgg cttgatcagc cagttggtca
       61 cccaggtgac gaacagacct ttcggatttt tgccgacgtt tctgatgctc tgaaaatcga
      121 ctttgagcat catgggatag atcatcagcc agatgaggat tgcaatcgga atggacacgt
      181 tggcatattc aaacttaccc agaaacgccg gaacgccggg cagaaactgg ccgatcagca
      241 cgcccgcagc catacagagg atgacccata cggtcaaata cttttcaaaa aagccgatac
      301 cagatgattt cttttccata ctcgacccct ccagcagaat aacctgttgt attttgttca
      361 aagcaagcga cgtttattgg ttgatctgat agatttcttc ctgctgcgct ttactgagcg
      421 acttttcagt aatcagcgca atgatcagga taccgatgat gctgagccca agccgcagaa
      481 gcgtaaacgt cagccccatg ctcgcccctt cgaacgcgag catcgggatt ttggtcgtcg
      541 accaggcgcc gataaagatg aacacgttga acaggctgct gccttttttg agcatgacgc
      601 ccgccaccgg aaacgccgcg tagagcggcc ccgccgcggc cgatccgagc agaaacgcaa
      661 tcagtacgcc cttgagcccg gagcctttgc cggtaaactt catcatggtc gcgcggtcaa
      721 cccaaacgtc cagcagtccc agcagaatga agatcggcgg aagcacggag agcatttcct
      781 tgagattctg cagcgtaatg gccacgctct ttttgccgat ttccggcagg aacagcccgg
      841 ttgccacgcc cgccgccaac agcaggaaaa acgcgccgta ccgcttcaaa aaattcttca
      901 tttcagcacc acccctatta cggccgccgc aatgagcgaa aagcccagcg cggaaacgtt
      961 gcggacgatc gccgcacgct tgccgaaggt tttcagctcc atcggcagag taatcacgcc
     1021 gaccatcatc agcgtggaaa caaacgccgc aatctgcatg taccccgcgc cgtttttcag
     1081 cagcgccgcc gcgagcggaa acgccacaaa cccgggaaca agcgtaatcg agccgatcaa
     1141 agccgccgcg agtacgccaa gcacgcctga gcgcgcgccg atcagttttt gaatcgtctc
     1201 cggactcagc accgccagcg caaagccgat aatcagcagg atggtcaaca gctgcggaag
     1261 gatgttttca aacgccttcc aagccttgac gagcgccatc ttagtctttt tccggtcctt
     1321 gaaaaacgag accgtaaggc cgataacggc cagcgcgtag agaatgtagg taaacatgct
     1381 ttctcccgtt ggcagagttt tatcggactt tcttcaaaat cgcgacgact tcctcggttt
     1441 tcagaacctt tccgtaggac acgactttgc cgtcaataac gagcgcgggc gtggtcatca
     1501 cgccgtagga agcgattttc gcatagtccg tgacgtgctc aatctccgtg tccattccga
     1561 gctgtgccag cgccgttttg gttgcaagct cgagggcgtt gcacttgggg catccgctgc
     1621 cgagtacctt tacggacgcg ccttcggtct tggcctgttc ggccttctcc atgctctcgc
     1681 agtcgcaagc gccgccgcag cagcaggagg atttcgtttc ttctttttct tttcgttttc
     1741 caaagagtgc catagtcgtt tctttccttt caatcaatcc aagtaaattg tttttctttt
     1801 ctatccatcc actaaaagcc taaatataac aaatatagtc caatcagcag gatcagcacg
     1861 cccgttgcga tcttcagcgc ccgggacagc gcgccatatc gctcactttt catcagctga
     1921 ttcaccatcc cgattgaaga
//
LOCUS       DAOMGY010000345         1925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_68588_length_1925_cov_2.262032, whole genome shotgun sequence.
ACCESSION   DAOMGY010000345 DAOMGY010000000
VERSION     DAOMGY010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68588_length_1925_cov_2.262032"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PLR57_05855"
     CDS             <1..700
                     /locus_tag="PLR57_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQQ41017.1"
                     /translation="EMKETTVLIGKQDRVCTLTINRPYIKNAWTVEFVEELRDAFRAV
                     GRDKDIHVVVLEGAGRDFSSGADYALFKAELSPSVWLDGMRTLKDLIVTIRGIPQPVV
                     AKVHVAVPRNSQVVLTVRTQLVPKSEAFEQSARISILCKLDNVGKNENEHPEQHQKQH
                     QLEKRVQVCRSAAALAFHRTGRQPGCAAPGGHPLRDEPDAHPGKEKRAGRRSEDCERV
                     AGSRNQHPARKNRE"
     gene            complement(857..1399)
                     /locus_tag="PLR57_05860"
     CDS             complement(857..1399)
                     /locus_tag="PLR57_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41018.1"
                     /translation="MEPIWIIIIIAWVIIPIIAKRKQQQAKEQAAREQAARQRAAQAA
                     TQQRVQQPMRTAPIAPTVRASVQSSFEGVTSGEGYGSKDEGSPTHQIGSTLQGKTTTL
                     KQIRVDPEHVVRATSESGHTHEETSMTGIEKECPPDAKPTAAGTASAPESAFVWDVSQ
                     VRNGLVLAEVLGPCLALRNE"
     gene            complement(1405..>1925)
                     /locus_tag="PLR57_05865"
     CDS             complement(1405..>1925)
                     /locus_tag="PLR57_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HQQ41019.1"
                     /translation="ANIDRLVGGAGEETIIARIGEGIVTTVGSSKSHKDVLENPDKIS
                     HTVLDKGLDAGTAFEILSIDIADVDVGRNVGAQLQIDQAEADKRIAQAKAEERRAMAV
                     AQEQENLAAVAGMRARVIEAEAEVPKAMAQAFRDGHLGIMDYYRMQNISSDTEMRTAI
                     GKGTKPAPADKK"
BASE COUNT          358 a          559 c          583 g          425 t
ORIGIN      
        1 cgaaatgaag gaaaccaccg ttttgatcgg caagcaggac cgggtctgca ccctgaccat
       61 caaccggccc tacatcaaga acgcctggac ggttgagttc gtggaggagc ttcgggacgc
      121 cttccgggcg gtcggacggg acaaggacat ccacgtcgtc gtcctggagg gggcgggacg
      181 ggatttttcc tccggggccg attatgcgct cttcaaggcg gaattgtccc cctccgtctg
      241 gctggacggg atgcggacgc tcaaggacct gatcgtgacc atccggggga ttccgcagcc
      301 ggtcgtcgcc aaggttcacg tagccgtccc gcgcaatagt caagtagtgc tgactgtccg
      361 tacccagctg gttccaaagt ccgaagcttt cgaacaatcc gcccgaatat ccattctgtg
      421 caagctggat aacgtaggca agaacgaaaa cgaacacccg gaacagcacc agaaacaaca
      481 tcagttggaa aaacgggtgc aggtttgccg atctgccgct gcgcttgcct tccatcggac
      541 gggcaggcag ccagggtgcg cggctccagg cggccatcca ctgcgggatg aaccggatgc
      601 ccatccaggc aaagagaaac gcgctggtcg acgcagcgag gattgcgagc gggtcgctgg
      661 gagccgaaac cagcacccag cgcgcaaaaa ccgcgaataa tacaatcaag ggtaccgatg
      721 taaacagtac ccttttccaa tattgcttat cttgtctcag ttccgaagga ttcatgcgtt
      781 atattatatc aattctgttg cgttttcaaa acgggttgga aagaatattt ccaacccgtt
      841 catattgtgt ttggagttac tcgtttctca gcgcaaggca ggggccgaga acttccgcga
      901 ggacgagccc gttgcgcacc tgagaaacat cccagacaaa cgcgctttcg ggagcgcttg
      961 ccgtgccggc agccgttggt ttggcatccg gcggacattc cttctcaatg ccggtcatgc
     1021 ttgtttcttc gtgcgtatgg ccggattcgc tggtagcccg gacgacgtgc tccggatcga
     1081 cgcggatctg cttgagcgtc gtcgttttac cctgcagcgt ggacccgatc tgatgcgttg
     1141 gagagccttc gtccttcgag ccatatcctt cgccggaagt aacgccttca aaggaggact
     1201 gcacgcttgc acgcacagtt ggcgcgatcg gagcggtgcg cattggctgc tgcacgcgct
     1261 gctgcgttgc ggcctgtgcg gctcgctgcc gggcggcctg ctcgcgagcg gcctgctcct
     1321 ttgcctgctg ctgctttctc ttcgcgatga tcgggataat cacccacgcg atgatgatga
     1381 taatccaaat tggctccata gcggttattt cttatcggcg ggggcgggtt tagtgccttt
     1441 gccgatggcc gtgcgcatct ccgtatccga ggagatgttc tgcatgcggt agtagtccat
     1501 gattccaaga tgcccgtcgc ggaacgcctg cgccatggct ttggggactt cggcttcggc
     1561 ttcgattacg cgggcgcgca tgccggcgac ggcagcgagg ttttcctgct cctgcgccac
     1621 ggccattgct ctacgctctt cggctttcgc ctgggcgata cgcttgtccg cttcggcctg
     1681 gtcgatctgg agctgtgcgc cgacgttgcg gccgacgtcc acatccgcga tgtcgatgga
     1741 gaggatttca aacgcggtgc ccgcgtcgag gcccttatcc agaaccgtat gggagatctt
     1801 gtccgggttt tccagaacgt ccttgtggct cttgctggag ccgacggtgg tgacgatgcc
     1861 ttcgccgata cgggcgatga tcgtctcttc acccgcgccg ccgacgagac ggtcgatgtt
     1921 cgcgc
//
LOCUS       DAOMGY010000346         1918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_68959_length_1918_cov_3.305421, whole genome shotgun sequence.
ACCESSION   DAOMGY010000346 DAOMGY010000000
VERSION     DAOMGY010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68959_length_1918_cov_3.305421"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLR57_05870"
     CDS             complement(<1..186)
                     /locus_tag="PLR57_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HQQ41020.1"
                     /translation="MEPLRKSVEQIVLAAGELLLEAAKTGTVVHEKEGVGNFVTDCDL
                     ATQQFLQRELKGLLPGSA"
     gene            complement(235..729)
                     /locus_tag="PLR57_05875"
     CDS             complement(235..729)
                     /locus_tag="PLR57_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HQQ41021.1"
                     /translation="MAAYGEAFTIQSDTRVTFHDVTDEVKRIIKQGSIQDGIVMVYSQ
                     HTTCSVLIQEQSDDVNFFGEQLVLQDMVNVLSKIVPTCETEGQYLHPGPLHVEIAARE
                     RDEKPWWSLNTDAHLRSVLLGRSVQIPVVKGELLLGEFGRIYFADFDQVRARQRTVRV
                     HMVG"
     gene            complement(847..1632)
                     /locus_tag="PLR57_05880"
     CDS             complement(847..1632)
                     /locus_tag="PLR57_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQQ41022.1"
                     /translation="MHSVVIIEDSFPLRQGMILTFDWRALNCYVAGEADNGEDGLRLI
                     REIKPEIVITDIRMPGMDGLDMIEELRKSGGDAVCIVISAYGEFDYAQRAIRFGVSEF
                     LVKPFETSELNSALKNAVRRADQILHLRQIEEKKVVLYGDYLAEEGDIKGKYAEQAVD
                     YIKAHYDESIGISDIALALSMSESHLSRVFKESMGYTLGEYLVNYRISVACELLREGK
                     LRVSEVAAKVGYGDQRYFSVVFRRIVGRTPNDYRAHAMNDRAE"
     gene            complement(1678..>1918)
                     /locus_tag="PLR57_05885"
     CDS             complement(1678..>1918)
                     /locus_tag="PLR57_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ41023.1"
                     /translation="VCDMLVVTIMDDGAGIPAERLERILSDELEEGDRESIGLKNVHN
                     RLKLYFGEPYGIRIESARGKGTTVTVHMPFQHQPE"
BASE COUNT          393 a          591 c          474 g          460 t
ORIGIN      
        1 cgcggaaccc ggcaaaagcc ctttcagctc gcgctgcaga aactgctgcg tcgccaggtc
       61 gcagtcggtc acaaagttgc cgacgccttc tttttcgtgc accacggttc ctgtttttgc
      121 cgcttccagc agcagttctc ccgcggcaag cacgatctgc tcaacactct ttctcaacgg
      181 ttccattccg cccacttcct tttattgcgg cgcccaccgc tccacacaat cttctcagcc
      241 gaccatgtgc acgcgcaccg tgcgctgacg cgcgcgaacc tgatcgaagt ccgcaaagta
      301 gatgcgcccg aattcgccca gcagcagttc gcccttgacc acgggaatct gcaccgaccg
      361 tccaaggagc accgagcgca aatgcgcgtc cgtattcaaa ctccaccagg gcttttcgtc
      421 gcgttcgcgc gcggcaattt ccacatgcag cgggccggga tgcagatact gcccctcggt
      481 ttcgcaggtg ggcacgatct tggaaagcac attgaccatg tcctgcaaaa ccagctgttc
      541 gccaaagaag ttcacgtcgt ccgactgctc ctgaatgagc acgctgcagg tcgtgtgctg
      601 ggaatacacc atcacgatgc cgtcctgaat cgaaccctgt ttgataatgc gtttgacctc
      661 gtccgtcacg tcgtgaaacg ttacccgcgt atcggactga atcgtaaaag cttctccgta
      721 agccgccatt gttcgtaatc ctcctgtaat gtttgataat tcttcgcacg tcagcacgcg
      781 cgttacataa attatctatt tatttactcg tttagtcaaa tcatttttat ttgtgtgttg
      841 agccgttcac tccgctctgt cgttcatcgc atgggcgcgg tagtcgttgg gcgttctgcc
      901 tacgatgcgg cgaaacacga cggaaaagta cctttgatcg ccgtagccta ccttggcggc
      961 aacttcggaa acgcgcagct tcccttcccg cagcagttca caggcaacgg agatacggta
     1021 attcacaagg tattcgccga gcgtataacc catgctctcc ttgaacacgc ggcttaagtg
     1081 gctctcgctc atggagagcg ccagcgcgat atcggaaata ccaatgctct cgtcataatg
     1141 cgccttgata tagtccacgg cctgctcggc gtacttgccc ttgatgtcgc cttcttcggc
     1201 gagataatcc ccgtagagca cgaccttttt ttcttcgatc tggcgcagat ggagaatctg
     1261 atcggctctg cggacggcgt ttttcagtgc gctgttcaat tcgctcgttt caaagggttt
     1321 cacgagaaat tccgaaacac cgaaacgtat ggcacgctga gcatagtcaa actcgccgta
     1381 ggcggagatc acgatgcaga ccgcgtcccc gccagatttt cgcagttctt ctatcatatc
     1441 gagcccgtcc atgcccggca tgcggatatc ggtaatcacg atttcaggct tgatttcgcg
     1501 gatgagccta agcccgtcct cgccgttgtc cgcttcgccc gccacatagc agttcagcgc
     1561 acgccagtca aacgtcagga tcatgccctg ccggagcgga aacgaatcct cgataatcac
     1621 tacgctgtgc atgcttcact ccccgaatga ctgcttggta aaatcaatga gccggtttta
     1681 ctccggctga tgctgaaacg gcatgtgcac cgttacggtc gtgcctttgc cgcgcgcgct
     1741 ctcaatgcga atgccgtagg gttcgccaaa gtagagcttg aggcggttat ggacgttctt
     1801 tagcccgatg ctctcgcggt cgccttcctc cagttcgtcg ctcaaaatcc gctccagccg
     1861 ctcggcggga atccccgcgc cgtcgtccat aatcgtaacg accagcatgt cgcaaacg
//
LOCUS       DAOMGY010000347         1916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_69102_length_1916_cov_1.758195, whole genome shotgun sequence.
ACCESSION   DAOMGY010000347 DAOMGY010000000
VERSION     DAOMGY010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69102_length_1916_cov_1.758195"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLR57_05890"
     CDS             complement(<1..483)
                     /locus_tag="PLR57_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQQ41024.1"
                     /translation="MNSKLNDLTALFDPEGIAVVGASNNPLKAGSVIIQNMAHGPMAN
                     RLYPVTGSEPVIAGLNAYKLLSEIPYKVELVVLIMPSSAIFSVMDDLDKRMSERGDVK
                     VIVCAAADYAETKTEEGARRQARLMQAKERYGIRVVGPNCIGAINNQNGVNTTIVITG
                     V"
     gene            complement(496..1410)
                     /locus_tag="PLR57_05895"
     CDS             complement(496..1410)
                     /locus_tag="PLR57_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQQ41025.1"
                     /translation="MTIIDKLATYEDQISPGISACVGCNVELTLRTCLKVLGPNTIFA
                     IPPGCMGGVGVVGWDKESGAKVPVFFPLLDNVASMLAGIKLHYDRVGRDVNVVAFAGD
                     GATVDAGFQCLSGAAERGARIIYICYDNEGYMNTGYQRSGSTTKGAWTSTTPVSKHGG
                     GGKKENKKDFPMIMAMHDLPYMATMNPAYIPDMVRKLEKAQEAVKHGLVYLHVYNPCV
                     TGWGFKSDESIELARLAVETNFAPLFEVEDKKFRLSVTVKNPKRVEEYVRRFKKFKHL
                     SDEEIAALQTLTDEKYERLLSLCQMQNR"
     gene            complement(1424..>1916)
                     /locus_tag="PLR57_05900"
     CDS             complement(1424..>1916)
                     /locus_tag="PLR57_05900"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQQ41026.1"
                     /translation="KLFDEIDQEYGRRFGRSYGGAIEEYRCDDAEFVLVTMGSMTGAA
                     KESVDRAREQGVPVGLVKIRLVRPFPAERIAKALAGKKAYGVIDRNVSFGWNTGIIYE
                     EIASAMNRAGQVVPSIPFICGLGGEDVTMAHVDHAIERIAECAAGKRFDETIWLNRVV
                     KAV"
BASE COUNT          364 a          557 c          559 g          436 t
ORIGIN      
        1 cacgccggtt ataacaatcg ttgtgttgac gccgttttgg ttgttgattg cccctatgca
       61 gttgggaccg acgacgcgga tgccgtagcg ctcctttgcc tgcatcaacc gcgcctgtcg
      121 gcgcgcgcct tcctccgttt ttgtttcggc gtagtcggcg gcggcacaga cgattacttt
      181 tacgtcgcca cgctcggaca tgcgcttgtc gagatcgtcc atgacggaga agatcgccga
      241 agaaggcata atcagcacga cgagttcgac tttgtacgga atctcggaga gcagtttata
      301 agcgttcagt ccggcgatga ccggctcgct tcccgtgacg gggtacagcc ggttcgccat
      361 ggggccgtgc gccatattct ggatgatgac gctgccggct ttaaggggat tattggaagc
      421 gccgacaacg gcaatcccct ccgggtcgaa cagcgcggtt aaatcattca gcttcgagtt
      481 caaagaggtg ccctcctagc ggttctgcat ctggcagagg ctgagtagac gctcgtattt
      541 ttcgtccgtc agggtttgca aagcggcgat ttcttcatcc gaaaggtgct tgaatttctt
      601 aaaccggcgg acgtattcct ctacgcgctt ggggttcttt acagtaacgg aaaggcggaa
      661 cttcttatcc tccacctcga aaagcggcgc aaagttcgtt tccaccgcaa gacgggcgag
      721 ttcaatgctt tcgtcggact tgaatcccca gccggtcacg cagggattgt aaacatgcag
      781 gtacacgagg ccgtgcttta ccgcttcctg cgctttttcg agctttcgca ccatgtccgg
      841 aatatacgcg gggttcatgg ttgccatata cggcagatca tgcatcgcca tgatcatggg
      901 gaagtccttc ttgttttcct tttttccgcc gccgccgtgc ttgctcacgg gtgtggtgga
      961 agtccatgcg cctttggtcg tggagccgga gcgctgatag ccggtgttca tgtagccttc
     1021 gttgtcgtaa cagatgtaga tgatccttgc gccgcgctcc gccgcaccgg aaaggcactg
     1081 gaagcccgcg tccacggttg cgccgtcgcc agcgaaagcg acaacattca cgtcccgccc
     1141 aacgcggtcg tagtgcaact tgatgcccgc gagcatggaa gccacattat cgagcagggg
     1201 gaagaacaca ggcacctttg cgccggattc cttgtcccag ccgactacgc ccacgccgcc
     1261 catgcatccc ggcgggatag cgaagatggt gttgggaccg agaaccttga ggcaggtgcg
     1321 cagcgtcagc tctacgttgc acccgacgca ggcggagatg cccggggaaa tctggtcttc
     1381 gtaagttgcg agcttatcaa taatcgtcat gattcatttc cccctacacc gctttgacga
     1441 cgcggttgag ccaaatggtt tcgtcgaagc gttttccggc ggcgcattcg gcaatgcgct
     1501 cgatggcatg atcgacgtgc gccatggtga cgtcctctcc gccaagcccg cagataaacg
     1561 ggatggacgg aacgacctga cccgcgcggt tcatggccga ggcgatttct tcatagatga
     1621 tgccggtgtt ccagccaaag gacacgttgc ggtcgattac gccgtaggcc tttttaccgg
     1681 cgagcgcttt tgcaatgcgc tcagcgggga agggacgcac gaggcggatt ttgaccagcc
     1741 cgaccggaac gccctgctcg cgggcgcgat ccacgctttc ctttgccgcg ccggtcatgg
     1801 aacccattgt gaccagcacg aactcagcgt cgtcgcagcg gtattcctca attgcgccgc
     1861 cgtagctgcg cccgaagcgc cggccgtact cctggtcgat ctcgtcgaaa agcttc
//
LOCUS       DAOMGY010000348         1913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_69264_length_1913_cov_2.755651, whole genome shotgun sequence.
ACCESSION   DAOMGY010000348 DAOMGY010000000
VERSION     DAOMGY010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69264_length_1913_cov_2.755651"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1039)
                     /locus_tag="PLR57_05905"
     CDS             complement(80..1039)
                     /locus_tag="PLR57_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HQQ41027.1"
                     /translation="MEQNSEQKKCYLAVDGGNSKTEYRLLDASGKVIASLRGCGSNHE
                     TMPGGFDDAADLLIEQCGQMLGANGYVLTDVQDAVLGLAGADYEEQSAALTACLQERG
                     LDKCFVCNDGYLGVMAGVKDGVGICYSAGSGVICAGMNQEGKRVQFGGIGVLCGDTGG
                     GFDIIAFVYRSVYQQVFFGKKRTALKDGYYTYFDIHSPAEFLASAVRLRNEESARQLI
                     GIFFTAVETADGLASRYAARAASHAADCIVAAANALSLEYPVHVALSGSIMTAVASKT
                     YRRMLEYELNRRKGDIFQLHTNDCPPVDGAVRWVRLRNGLKNE"
     gene            complement(1115..>1913)
                     /locus_tag="PLR57_05910"
     CDS             complement(1115..>1913)
                     /locus_tag="PLR57_05910"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HQQ41028.1"
                     /translation="GLCNLPIGTQERIAKLLGVPMESVRLETVSLNHCGAVTAVYLGG
                     KNVLPKLLSPESLDQAEALDPWVKNYRPIIELLGAIPNDYLQYFFYRDRKFQELLKAE
                     QTRGEACQEIETRLLSYYADPRNNTVPAMLAERGGHLYSEAAIALIASLSGAEPGHHV
                     IDVQNNGVLNFLGGASVIETSCFVSRGRIERNPIESKPNPVLTSLVSSVKAYETLTVQ
                     AALSGDRNTALRALACNPITADLDKAAPCLEEMLLQNRPLLPRFFPV"
BASE COUNT          400 a          537 c          566 g          410 t
ORIGIN      
        1 ttaagaaaag aagagaaaaa agctaattct tttgcttctt ttcttctaat aaaagaagag
       61 cttattcgaa aagaagagct tattcgttct ttagcccgtt ccgtaaccgc acccagcgca
      121 ccgcgccgtc cacgggcggg cagtcgttgg tatgcagctg gaaaatatcg cctttgcggc
      181 ggttaagctc gtattcgagc atcctgcggt aggtcttgga agcgaccgcc gtcataattg
      241 aacccgaaag ggcgacgtgc accggatatt ccagcgagag cgcgttggca gcggcaacga
      301 tgcagtccgc cgcgtgagag gcggcgcggg cggcgtaacg ggaagcaagg ccgtccgccg
      361 tttccaccgc ggtaaagaaa ataccgatca gctggcgcgc ggattcttcg tttcgcaacc
      421 gaacggcgga agcgagaaac tccgcggggg agtggatatc gaagtaggta tagtagccgt
      481 ccttgagggc ggttctcttt ttaccgaaaa agacctgctg atagaccgac cgatagacaa
      541 acgcgatgat atcaaacccg ccgcccgtat cgccgcagag cacaccgatg ccgccgaact
      601 gaacgcgctt gccttcctga ttcatgcctg cacagattac gccggaaccc gcggagtagc
      661 agatccctac gccgtccttt acgcccgcca ttacgccgag atacccgtcg ttgcagacaa
      721 agcatttgtc gagcccgcgc tcctgcaggc aggcggtgag cgccgcgctc tgctcttcgt
      781 aatccgcgcc ggcaagaccc agaaccgcgt cctggacatc ggtcagcacg tagccgtttg
      841 cgccgagcat ctgaccgcat tgttcaatca gtaaatccgc cgcgtcgtca aacccgccgg
      901 gcatcgtttc atggttgctg ccgcagcccc tgagcgaggc gatcactttt ccggacgcat
      961 cgagcaggcg gtattcggtc ttggagtttc ctccgtcaac ggcaaggtaa cacttctttt
     1021 gttccgaatt ttgttccata ctgcggcaga acctcgatta gattttttcc tgagctgtgg
     1081 taggattttc agacatttac tctggattgg cgaatcagac ggggaagaag cggggcagca
     1141 gcggccggtt ttgcaaaagc atctcttcca gacagggagc cgctttgtcg agatccgcgg
     1201 tgatggggtt gcaggccagc gcacggagcg ccgtgttgcg gtcgccggac agcgccgcct
     1261 gaacagtcag cgtttcgtaa gcctttacgc tggaaacaag cgaggttaga accgggttcg
     1321 gcttgctttc gatgggatta cgctcaattc ttccgcggga tacgaagcag ctggtttcga
     1381 ttacgcttgc gccaccgaga aaattcagca caccgttgtt ctgcacgtcg atgacatgat
     1441 gcccgggttc cgcgccggaa agagaggcaa tcagtgcaat ggccgcttcc gagtagaggt
     1501 gcccgccgcg ctcggccagc atggccggaa cggtgttgtt gcgcgggtcg gcataatacg
     1561 acagcagccg cgtttcgatt tcctggcagg cttcgccgcg cgtttgctcc gctttgagca
     1621 gctcctgaaa tttgcggtcg cggtagaaga agtattgcag gtaatcgttc ggaattgcgc
     1681 cgagcagctc gataatcggg cggtaatttt ttacccatgg gtcgagcgcc tccgcctgat
     1741 ccagcgattc cggcgagagg agcttgggca gaacgttttt gccgcccaga tagaccgccg
     1801 tgaccgcgcc gcagtggttg agggaaacgg tttccagccg cacgctctcc attggaacgc
     1861 cgagcagttt tgcaatgcgt tcctgcgtgc cgatgggcag gttgcaaagc ccg
//
LOCUS       DAOMGY010000349         1912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_69316_length_1912_cov_3.052235, whole genome shotgun sequence.
ACCESSION   DAOMGY010000349 DAOMGY010000000
VERSION     DAOMGY010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69316_length_1912_cov_3.052235"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1662
                     /gene="cimA"
                     /locus_tag="PLR57_05915"
     CDS             58..1662
                     /gene="cimA"
                     /locus_tag="PLR57_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HQQ41029.1"
                     /translation="MNKVLLLDSTLRDGAQSVGISFSVQDKLKIARLLDNLRVDYIEA
                     GNPGSNPKDMEFFELLRSHPLRHAKLTAFGSTRRKHIAVEDDANIRSLLAAGTECVTI
                     FGKSWDIHVTEILRAELEENLRMIAETVAYLKASGKEVVYDAEHFFDGYKANPDYALQ
                     TLEAAVQGGADCLCLCDTNGGTFPDDVYEATRLVAARFPVAVGIHAHDDSGMAVANSI
                     MALKAGAVHVQGTLAGFGERCGNANLATILANVQLKLGVACVPPDCLERLTHTVHEVA
                     EISNISVHKNTPYVGGNAFSHKGGMHVDGVMKNTLTFEHVPPSSVGNTRRLLLSEVAG
                     RSAILEKVRAFCPEIQKDSPEAAHIIDMVKQMEHQGYQFEGAESSFELEVRRMLGKST
                     KFFELLMLSVLDEQSLDGGKKPAYATIKVLVGDQLEITAAEGNGPVNAIDIALRKALE
                     VFYPEIGEIRLTDYKVRVLDSESATAAKVRVLIESTDGSEYWTSVGVAVDVMQASCKA
                     LMDSIEYKLLISNAVPKQPAASAANA"
     gene            1721..>1912
                     /locus_tag="PLR57_05920"
     CDS             1721..>1912
                     /locus_tag="PLR57_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HQQ41030.1"
                     /translation="MKLTGAQILIECLAEQSVDTVFGYPGGAVLNIYDELYKNSHRIH
                     HYLTSHEQGAAHAADGYARA"
BASE COUNT          450 a          555 c          569 g          338 t
ORIGIN      
        1 agaaatgagt cgaagtcagc ccgcgggagg cggaactgga aaccgggagg gtcgattgtg
       61 aacaaggttt tgttgctgga ttccacactg cgcgacggcg cgcagagcgt aggcatttcg
      121 ttttccgtgc aggacaagct gaagattgcg cgtctgctcg acaacctccg cgtggattat
      181 atcgaagccg gaaaccccgg ctccaacccc aaggacatgg agttctttga gctgctccgc
      241 tcgcacccgc tgcgccacgc caagctcacg gcgttcggca gcacccggcg caagcacatc
      301 gcggtggaag acgacgcaaa catccgctct ctgcttgccg cagggacgga atgcgtcacc
      361 atcttcggca aatcctggga tatccatgta actgagattc tgcgcgcgga actggaagaa
      421 aatctccgca tgatcgcgga aaccgtagct tacctgaaag ccagcggcaa ggaagtcgtc
      481 tacgacgcgg agcatttttt cgacggctac aaggccaacc ccgactatgc cctgcaaacg
      541 ctggaagccg ccgttcaggg cggcgcggac tgcctctgcc tctgcgatac gaacggcgga
      601 acctttccgg acgacgttta cgaagcaacc cggctcgtcg ccgcgcggtt tcccgttgcc
      661 gtaggcattc atgcgcacga cgacagcggc atggcggttg ccaacagcat catggcgctg
      721 aaggcgggcg cggtgcacgt acagggaacg ttggcgggct ttggcgagcg ctgcggcaac
      781 gcaaaccttg cgaccattct ggcaaacgtc cagctcaagc tgggagttgc ctgcgtaccg
      841 ccggactgcc tggaacgact tacgcacacc gtccacgaag tagcggaaat ctccaacatc
      901 agcgttcata aaaacacgcc atatgtcgga ggaaacgctt tctcgcataa aggcggtatg
      961 catgtggacg gtgttatgaa aaacacgctg acgttcgagc acgtcccccc ttcctccgtt
     1021 ggaaacacgc ggcggctgct gctttcggaa gtggcaggcc gcagcgcgat actcgaaaag
     1081 gtgcgcgcgt tctgcccgga gattcaaaag gactcgccgg aagccgcgca tatcatcgat
     1141 atggtcaagc agatggaaca tcagggctac cagttcgagg gcgcggaatc ctcctttgag
     1201 ctggaagtac gcaggatgct gggcaagagc accaagttct ttgaactgct gatgctcagc
     1261 gtactcgacg aacagtccct cgacggcggg aaaaagccgg cctacgcgac catcaaggtg
     1321 ctggttggcg atcagctgga gattaccgcg gcggaaggca acggcccggt caatgccatc
     1381 gacatcgcgc tgcgcaaggc actggaagta ttctatcccg aaattggcga aatccggctg
     1441 accgactaca aggtgcgcgt actcgactcc gagtccgcaa ccgccgcaaa ggtacgcgtg
     1501 ctgattgaat cgaccgacgg cagtgagtac tggacgagcg taggcgtagc ggtggacgta
     1561 atgcaggcaa gctgcaaagc gctgatggac tccattgagt acaagctctt aatcagcaac
     1621 gccgtaccga agcagcccgc cgccagcgcg gcaaacgctt aacccgccgc gcaggtaaaa
     1681 agcaccatgc agacaggacg acaaaaagga gacccgtttt atgaaactga caggagcgca
     1741 aatcctgatt gaatgtttgg ccgagcagtc cgttgatacc gtgttcgggt atccgggcgg
     1801 cgcggtgctg aatatttacg acgaactcta taaaaacagc catcgtattc accattacct
     1861 gacctcacac gagcagggcg cggcgcacgc cgcggacggc tatgcgcgcg cg
//
LOCUS       DAOMGY010000350         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_69507_length_1909_cov_2.920712, whole genome shotgun sequence.
ACCESSION   DAOMGY010000350 DAOMGY010000000
VERSION     DAOMGY010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69507_length_1909_cov_2.920712"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="PLR57_05925"
     CDS             complement(<1..1309)
                     /locus_tag="PLR57_05925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ41031.1"
                     /translation="MFKNKKFANAIFILATVSSIIYLLWRAFFTLPLHNGAVSLVFGI
                     LLLGSEIAAALGTYELYWRKSHLNRMDIKRPEIPLDWYPDVDVLIATHNESVELLYKT
                     VNAITFLDYPDKRKVHVYLSDDGNRPEVAELAARLKVGYIGLANNKQAKSGNYNNALK
                     HTSSPLVATFDADMIVRRNFLMETVPYFYLPRVKRLEDGTWVPREESEIDPRYKIGFI
                     QTPQSFYNPDLFQFNLYAEHNIPNEQDFFSREINVMRNSSNAVAYTGSNTVLSRAALE
                     EIGGFPTDTITEDFETGIRIQSKGYTAYSTTEPLASGLAPTSIKSMVTQRVRWARGVI
                     QSIRNTKLLTNKGLTVDARISYMVSYSYWWSFARRLVFTFAPIMFALFGQQVVITTFW
                     QIMAIWAPSYIFYSLAMRSLSSDTRNQRWNQIIDTILAPFMVIP"
     gene            complement(1694..>1909)
                     /locus_tag="PLR57_05930"
     CDS             complement(1694..>1909)
                     /locus_tag="PLR57_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41032.1"
                     /translation="SLNPPGKFILPLWLFYGNHVFDTPARNRCKKYLRQIKLKSEEPC
                     RVVVFWSSGKTILETRLFRYISVILSL"
BASE COUNT          404 a          515 c          520 g          470 t
ORIGIN      
        1 ccggaatcac cataaacggc gcaaggatcg tatcgataat ctggttccag cgctggtttc
       61 ttgtgtcgct cgaaaggctg cgcatcgcca ggctgtagaa gatatacgaa ggcgcccaga
      121 tcgccatgat ctgccagaac gtcgtgataa cgacctgttg gccaaacagc gcgaacatga
      181 tcggcgcaaa ggtgaacaca agccttctgg caaacgacca ccagtaggaa tacgaaacca
      241 tgtagctgat gcgcgcgtcc acggtgagcc ccttgttcgt cagcagcttg gtattgcgaa
      301 tggattgaat cacgccgcgc gcccagcgca cccgctgggt gaccatgctc ttgatgctgg
      361 tcggggcgag gccgctggcc agcggttcgg tcgtggaata cgccgtgtag cccttggact
      421 gaatccggat gcccgtttca aagtcctcgg taatcgtatc cgtcgggaac ccgccaatct
      481 cctccagcgc ggctctcgaa agcaccgtgt tgctgccggt ataggcaacg gcgtttgagc
      541 tgttgcgcat aacgttgatc tcgcgcgaga agaaatcctg ctcgttcggg atgttgtgct
      601 cggcgtagag gttaaactgg aacaaatccg ggttatagaa gctctgcggc gtctgaataa
      661 acccgatttt gtagcgcggg tcgatttcgc tctcttcacg cggcacccag gttccgtcct
      721 cgaggcgctt gacgcgcggg aggtagaagt acggcaccgt ctccattaag aagttgcggc
      781 gcacgatcat atccgcgtca aacgttgcca ccagcggcga actcgtatgc ttcagcgcgt
      841 tgttgtagtt gccggatttg gcctgcttgt tgttagcaag acctatgtag cctaccttca
      901 gcctcgcggc gagctccgcc acttccgggc ggttgccgtc gtcgctgaga tagacgtgca
      961 ccttgcgctt atccgggtaa tccagaaacg ttatggcgtt gaccgtcttg tagagcagtt
     1021 cgaccgactc gttatgagta gcgatcagca cgtccacatc cgggtaccag tccagcggta
     1081 tctccgggcg cttgatatcc atcctgttga ggtggctctt gcgccagtag agttcgtatg
     1141 tgccaagcgc cgcggcaatt tcgctgccca gcagcagaat accgaacacc agagaaacgg
     1201 cgccgttgtg gagcggcagc gtgaaaaacg ctctccacag taaatagata atcgacgaaa
     1261 ccgtcgcaag aataaagatc gcgttagcga attttttgtt tttaaacatt cggatcactc
     1321 ctctttgttg cttttgttgg gagcaactgg attccccgac ggtcagcaag ttcctttcgc
     1381 gcctcgctga cgattccatt ccctttgtgc gtttggccta aaggccgttt tgctgtttgc
     1441 cgtgcaagct gcggcaaacc cgtatatccg gtatcttcgg acgggcggat cgtatccgcg
     1501 ctttgtccgg gttatgtctg tcttgttccc gcgagtgtcc gcgccatgtg aagcctgttg
     1561 cttccgcggc gcctgtgccg ttcggggaaa cggacccgcg tctcccggct gtgtttcatg
     1621 cgagttttac tgcgtatgaa tatcctgccg gtacatcatt tattacttac atcatatcta
     1681 tgtaaaggcg cattcacagt gataaaatca cgcttatgta acgaaacaag cgtgtttcca
     1741 aaatcgtctt tccactgctc caaaaaacga cgacccggca cggctcttct gattttagtt
     1801 tgatctgtcg caagtatttt ttacaacggt ttcgggccgg cgtgtcgaaa acatggttgc
     1861 cataaaataa ccaaaggggt aaaatgaact taccgggcgg gtttaaact
//
LOCUS       DAOMGY010000351         1908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_69553_length_1908_cov_3.781436, whole genome shotgun sequence.
ACCESSION   DAOMGY010000351 DAOMGY010000000
VERSION     DAOMGY010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69553_length_1908_cov_3.781436"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLR57_05935"
     CDS             complement(<1..598)
                     /locus_tag="PLR57_05935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HQQ41033.1"
                     /translation="MAIPETQLYARSDKQTVYLKNAVKNPNIVVGDYTFYNDWERDPA
                     EFERHNVLYHYPVNGDRLIIGKFCSIACGTKFLFTSGNHAKKSLANYPFPIFADAWGL
                     DWANVAEAWDNKGDTVIGSDVWIGYEAVILQGVHIGDGAVVGARAVVTKDVPPYTIVG
                     GVPARELGKRFPPETVECLLKLRWWDWPERLKRASCRKR"
     gene            846..1193
                     /locus_tag="PLR57_05940"
     CDS             846..1193
                     /locus_tag="PLR57_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQQ41034.1"
                     /translation="MPENETLQSLVTELRRGTLTLAVLGALRKPRYGYALLQTMQQEN
                     IEIEANTLYPLLRRLESQGLLSSDWDTSESRPRKYYTVSARGGEVLKELLVEWDKMQQ
                     SIASVCGKDEKNG"
     gene            1186..>1908
                     /locus_tag="PLR57_05945"
     CDS             1186..>1908
                     /locus_tag="PLR57_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41035.1"
                     /translation="MDNLIERYVQEVTRRLPEKERAEVSRELKSNIYDMLPEHPDEEA
                     IKKVLNSLGSPSALAEKYRQNPRYLISPAYFDEYTRTLKWVLPLVGLVVMAVGMLVGA
                     LEAMKVSAGSVQEIAFSISNIISKGISMGVSAAFQALVWTTVGFVIADHAGKKNLSSK
                     EHVWKVEDLPELQPNEKTRIPLSDGIAELVVTVVFSILGILFCLGAFPFAMIIIDGQM
                     QVTNIFSAEFLAALIPVIAVTAV"
BASE COUNT          444 a          503 c          545 g          416 t
ORIGIN      
        1 tcctcttccg acaactcgct ctctttagcc tttccggcca gtcccaccag cgcagcttca
       61 gcaggcattc caccgtttcc ggcggaaagc gcttgcccag ttcgcgcgca ggcacgccgc
      121 cgacgatcgt gtacggcggt acgtcctttg tgaccaccgc gcgagcgccg acgactgcgc
      181 cgtcgccaat gtgcacgccc tgcaggatga cggcttcgta cccgatccac acgtcggaac
      241 cgatcaccgt gtcgccctta ttgtcccagg cttcggcaac gtttgcccag tcaagccccc
      301 aggcatctgc gaatatgggg aaggggtagt tggccagcga cttttttgcg tggttgccgc
      361 tggtaaagag aaactttgtg ccgcaggcga tggagcagaa cttgcctatg atgagccgat
      421 cgccgttgac ggggtagtgg tacagcacgt tatgccgctc aaactccgcc gggtcgcgct
      481 cccagtcgtt gtagaacgtg taatcgccaa cgacgatatt ggggtttttc accgcgttct
      541 tcagatagac ggtctgcttg tcggaacgcg cataaagttg agtttccgga atagccataa
      601 tcgttttcct cgattcgttt atttttggct gactattccg gcatggacaa tgcagcgctt
      661 catgcgcgcg cctcctttga tagtatgatt actaccacag tataagccaa ttcttccgtc
      721 ctgaaaacat acgataataa gctcagccaa acaacgattg cgatttcata tacatgtaga
      781 atatataata aaaatgtatt gacaatgatg tgcgacgcac agtataatac gtccgaggtg
      841 ataccatgcc ggaaaatgaa acgctgcaga gccttgttac agagctcaga cgcggcacat
      901 tgacgctggc ggtgctcggc gctttaagaa aaccgagata cggctacgcg ctgctgcaga
      961 cgatgcagca ggagaacatc gaaatcgaag ccaatacgct ctatccgctg ttaaggcggc
     1021 tcgaaagcca gggactctta agcagcgact gggatacgag cgagagcagg ccgagaaagt
     1081 actataccgt cagcgcgcgg gggggagagg ttctaaagga acttcttgtg gaatgggata
     1141 agatgcagca gagcatcgca tccgtttgcg gaaaggatga gaagaatgga taacctgatt
     1201 gaacgctacg tgcaggaggt aacgcgccgc cttccggaaa aagaacgcgc ggaggtcagc
     1261 cgggaactga agtcgaatat ctacgatatg ctgccggaac acccggacga agaagcgatt
     1321 aaaaaagtgc tcaacagcct cggttcgcct tcggcgctgg cggagaagta ccgtcaaaac
     1381 ccgcgctatc tcatttcgcc ggcgtacttt gacgaataca cccgcacatt aaagtgggtg
     1441 ctgccgttgg tgggccttgt ggtgatggca gttggcatgc tcgttggcgc gctggaagcc
     1501 atgaaggtca gcgcgggcag cgtgcaggaa attgcgttca gcatcagcaa catcatctcc
     1561 aagggtatca gcatgggcgt ttccgcagca ttccaggcgc tggtctggac gacggtcggg
     1621 ttcgtgattg ccgaccacgc gggcaagaag aacttgagct cgaaagagca tgtctggaag
     1681 gtggaagacc tgccggaact ccagccgaat gaaaaaacgc gcattccgct ttcggacggg
     1741 attgcagagc tggtcgtgac ggtcgtattt tcgattctcg gcattctgtt ctgcctgggg
     1801 gctttcccgt ttgccatgat catcatcgac gggcaaatgc aggtaaccaa tatcttcagc
     1861 gcggaattcc tcgcggcgct gattccggtc atcgccgtga cggctgtg
//
LOCUS       DAOMGY010000352         1900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_69990_length_1900_cov_3.204336, whole genome shotgun sequence.
ACCESSION   DAOMGY010000352 DAOMGY010000000
VERSION     DAOMGY010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69990_length_1900_cov_3.204336"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PLR57_05950"
     CDS             complement(<1..504)
                     /locus_tag="PLR57_05950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQQ41036.1"
                     /translation="MIDTIFFDIGNTLRVVVKDKAFSDAAEQEMMRLVGTTESHDAFF
                     EKLNARWEKYRKAAKNGHLDASEMELWSQYLLPDYPIDLICAVAGKLTRLWRDHDGRR
                     VARPDVKSTLVELDKRGYTMGIIANTITETEIPDWMIEDGVARYFKTVILSSKVRLRK
                     PDPEIYRL"
     gene            complement(654..1418)
                     /locus_tag="PLR57_05955"
     CDS             complement(654..1418)
                     /locus_tag="PLR57_05955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQQ41037.1"
                     /translation="MIDVVLFDIGGTVHTVTNNEALRTAFAKRLRERLSDYGILLEGD
                     DRQIGLRLHENAEEYKHYSEQILGELPQVRIWNEFYLKDYRIGAERLLPNAEELSFLY
                     DYERVRNMRRPRLVETFESLKAMGMRMGIISNIISTSFVPHILNEYGIAKYMECVVLS
                     SEAVVRKPNPAIFDIALEQLGAKREHTAYVGDTLSRDVLGARNANLGLMIQINNPAIA
                     HRDAGLKEKGVEPDVRIMEFSEIPGVVAAFNKAHPE"
     gene            1792..>1900
                     /locus_tag="PLR57_05960"
     CDS             1792..>1900
                     /locus_tag="PLR57_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQQ41038.1"
                     /translation="MKSVTIKDIAKVANVSYATVSRALSGSTEIGEETRE"
BASE COUNT          424 a          515 c          480 g          481 t
ORIGIN      
        1 caaacggtaa atttccgggt cgggcttgcg gaggcgcacc ttagaagaga gaatgacggt
       61 tttaaagtac cgcgcaacgc cgtcctcaat catccagtcc ggaatctcgg tttcggtgat
      121 cgtgttggcg ataatgccca tggtgtagcc gcgtttatcg agctcgacga gcgtgctctt
      181 tacgtccggc cgcgccacgc gccgtccgtc gtgatcccgc cagaggcgcg taagtttgcc
      241 cgcaacggcg cagatcaaat cgatcgggta atccggcagg aggtactgcg accagagctc
      301 catttcggaa gcgtcgaggt gtccgttctt cgcggctttg cgatacttct cccagcgcgc
      361 gttgagcttt tcaaaaaacg cgtcgtgcga ttccgtcgtg ccgacaagac gcatcatctc
      421 ctgctcggct gcgtccgaaa acgctttgtc cttgacaacg acgcgcagcg tattgccaat
      481 gtcgaaaaaa atggtgtcga tcatgccctg tagcctcctt gtatttgctt aacgttttcc
      541 agtatgtatt tcaatttcac attgggatag ctttcgaatt cggcagaaga attgaatggc
      601 tttgcggttg gattatttgc gtttacggtt tgcctgacaa cagaaaacct gctttactcc
      661 ggatgcgctt tgttgaacgc ggcgacaacg cccggtatct cggaaaactc cataatccga
      721 acatccggct ccacgccttt ttccttcagc cccgcgtccc ggtgcgcaat ggccggattg
      781 ttgatttgaa tcatcagccc aaggtttgcg tttctcgcgc ccagaacgtc ccgcgagagc
      841 gtgtccccca cataggccgt gtgctcccgc tttgcgccga gctgttccag cgcgatatcg
      901 aagatggccg ggttgggctt gcgcacgacc gcctcgctgg agagcacgac gcactccata
      961 tacttagcaa taccatattc gttgaggata tgcggcacga acgaagtgga gatgatgttg
     1021 ctgataatac ccatacgcat gcccatcgcc ttgagggatt cgaacgtttc gaccaaacgc
     1081 ggacggcgca tgttgcgcac gcgctcgtaa tcatatagaa agctcagttc ctcggcgttc
     1141 ggtaaaagcc gctctgcgcc aattctatag tcctttaagt aaaattcgtt ccagatgcgc
     1201 acctgcggca gttcgccgag tatctgttcg gaatagtgct tgtattcttc cgcgttttcg
     1261 tgcagcctca gcccgatctg cctgtcgtcg ccttccagca ggattccgta atcggacaga
     1321 cgctctctga ggcgtttggc aaacgccgta cggagcgctt cgttgttggt tacggtatga
     1381 acggtcccgc cgatatcaaa gaggacgacg tcgatcatga ttggttcgcc tttccagcaa
     1441 ataacgttga aactgtggga aaagtcgtaa acgtttgcgt caacacttta gcttgattct
     1501 aatcgatctg gatttttttg tcaatatgca gagttactgg tttctgcata tcacgctctt
     1561 ttgttgcaag ccgcaattct gcgcaaaaga atgaacattg acaaatgatt ttttgcatag
     1621 taatatcaat ttacggcgga aacgtttaca caataaaaat acccgttttt ccgcgtcatc
     1681 cacccgcctc caacccgttt cctcatagta aaaaaattgc cgatcagaaa tggtcgcagc
     1741 gtcgcgtccc ggccgtccgc atgaattata cgcgaaagcg aggacttgca tgtgaagagc
     1801 gtcaccatta aagatatcgc caaagttgcc aatgtttcct atgcaaccgt ttcccgcgcg
     1861 ctctccggca gcaccgaaat aggcgaggaa acgcgcgagc
//
LOCUS       DAOMGY010000353         1897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_70197_length_1897_cov_2.241042, whole genome shotgun sequence.
ACCESSION   DAOMGY010000353 DAOMGY010000000
VERSION     DAOMGY010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70197_length_1897_cov_2.241042"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..913
                     /locus_tag="PLR57_05965"
     CDS             215..913
                     /locus_tag="PLR57_05965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ41039.1"
                     /translation="MQRIHPRERLACAMELLGGEKVVADIGCDHGRLSCALVQSGRAE
                     RCIAVDISQPSLKKAEQLAAQLGVFDRVETRLGDGLAPLTTGEADALAILGMGGTLMV
                     RILEAPNTPLAGANLCVLQPMRAAEDIRRWLYERKYKVIDDRVVLDAGRYYQIFSVAP
                     PADARQALPAGWPVDCFLLGYKAFERCEPNLFRLAERMLQTTERKRKTQKSELLDRQA
                     EQLRQILSFREELS"
     gene            910..1671
                     /locus_tag="PLR57_05970"
     CDS             910..1671
                     /locus_tag="PLR57_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HQQ41040.1"
                     /translation="MKLSEFTAVMERIAPQDLAMEFDNPGLLIEPDHDEIKRVLVALD
                     CSKAVAEEATELGVDLVLTHHPLFFHPVKRLWYSQPDTAAACMLLRHGIGLFSAHTNL
                     DAAQGGVNDTLCELMHITDTIPFDEGIGRVGLLKEPVTLSEFASRAEQLLQTSVRLCG
                     SPEALVRRVAVMGGSGGSSIHPAAQERADVLLTGEIKHSDALDAQVFGLNLVIAGHYE
                     TERVVLAPLIRRLQNECFNVQYNLSRADVNPFVRL"
     gene            1684..>1897
                     /locus_tag="PLR57_05975"
     CDS             1684..>1897
                     /locus_tag="PLR57_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41041.1"
                     /translation="MRKMDALWAYQEAEVAKASVEAEIRSTPARLKLNKLHKQLKTQQ
                     GVITKLGEDIETYEQQLNVYAYIWQEL"
BASE COUNT          428 a          506 c          561 g          402 t
ORIGIN      
        1 gccctcaagt ctgcgatttg ttcgagggct ctgccctcga gctcccgacg aagggcagca
       61 ccattaaact cacgatataa acttttctga aaagacaaat caatcagctt ctcggattac
      121 gagttagaat ttgcggaaat gacacaatgc aaactggtga gcggtttgca ttttgttttg
      181 atgtttttta taataaattt gtatggaagt atcaatgcag cgaattcatc cccgcgagcg
      241 ccttgcctgc gcaatggaac tgctcggcgg cgaaaaagtg gttgccgata tcggctgcga
      301 ccacgggcgg ctgagctgcg cgctcgtgca gagcggcaga gcggaacgct gtatcgccgt
      361 cgatatcagc cagccttcgc tcaaaaaggc ggaacagctc gccgcacagc tcggcgtttt
      421 tgaccgtgtg gaaacccgcc tcggcgacgg tctcgctcct ctgacaacgg gcgaagcgga
      481 cgcgctcgcg attctcggca tgggcggcac gctcatggtg cgtatccttg aagcgccaaa
      541 tacaccgctc gcgggagcga atctctgcgt gcttcagcct atgcgcgcgg cggaagatat
      601 ccgcaggtgg ctctatgagc gcaagtataa agttattgac gaccgcgttg tgctggacgc
      661 aggaaggtac tatcaaatct tctcggttgc gccgccagcg gatgcgcgtc aggcgcttcc
      721 ggcgggctgg ccggtagact gcttccttct cggctacaaa gcgttcgagc ggtgcgaacc
      781 gaacctgttc cggctggcgg agcggatgct ccagacaacc gagcgaaaac gcaaaaccca
      841 gaaatccgaa ctgttggacc ggcaggcgga acagctccgc cagattctat cgtttcggga
      901 ggaattatca tgaaactcag cgaatttacc gccgtcatgg aacgtatcgc cccgcaggat
      961 ctcgccatgg agttcgataa tcccggtctg ctcattgagc cggatcacga cgaaatcaag
     1021 cgcgtgctgg ttgcgctgga ctgctcaaaa gcagtcgccg aggaagcaac cgagttgggc
     1081 gttgatttgg tgctcacgca tcatccgctg ttctttcatc cggtcaagcg gctttggtac
     1141 agtcagccgg atacggcggc ggcctgcatg ctgcttcggc acggaatcgg cctgtttagt
     1201 gcgcatacca atctggacgc ggcgcagggt ggcgtaaacg atacgctctg cgagctgatg
     1261 cacattaccg ataccatccc gttcgacgaa ggcatcggtc gcgtgggact gcttaaggag
     1321 ccggtgacgc tctcggaatt cgcttcgcgc gcagaacagc tattgcagac gagcgtgcgt
     1381 ctctgcggaa gcccggaagc gcttgtacgc cgcgtcgctg tgatgggcgg cagcggaggg
     1441 agctccattc accccgcagc gcaggagcgg gcagatgttt tactcacggg agaaatcaag
     1501 cattctgacg cgctggacgc gcaggtgttc ggccttaatc tggtgattgc cgggcattac
     1561 gaaaccgagc gagtggtgct tgcgcctctg attcgacgtt tacagaacga atgttttaat
     1621 gtacaatata atttatcgcg cgcggacgtg aatccattcg tgcgccttta ggagggaaat
     1681 gccgtgagaa agatggatgc cctttgggca tatcaggagg cagaagtggc taaggcttcc
     1741 gtagaagccg aaatccgctc tacgccggcg agattgaaac tcaataaact gcataagcag
     1801 ttaaaaacgc agcagggtgt tataacgaaa cttggcgagg acatcgaaac ctacgagcag
     1861 cagctcaacg tctatgccta catctggcag gagctgc
//
LOCUS       DAOMGY010000354         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_70283_length_1895_cov_3.383696, whole genome shotgun sequence.
ACCESSION   DAOMGY010000354 DAOMGY010000000
VERSION     DAOMGY010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70283_length_1895_cov_3.383696"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLR57_05980"
     CDS             <1..684
                     /locus_tag="PLR57_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQQ41042.1"
                     /translation="ARKPKGLAVISEINGVVHIDDSKKRKEAVVTNEDGEAESYLIPF
                     GSKLKVSDGDVVEAGDELTEGSVNPHDILKIKGVKGVQAYLLKEVQSVYRLQGVEISD
                     KHIEVIIRQMLRKVQVEENGETTMLPGELIDIFRFEEENERVVQNDGKPAIAKRKLLG
                     ITKASLATDSFLSAASFQETTRVLTEAAIKGKIDPLVGLKENVIIGKLIPAGIGLRRY
                     RNVNVTTKD"
     gene            719..1453
                     /locus_tag="PLR57_05985"
     CDS             719..1453
                     /locus_tag="PLR57_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41043.1"
                     /translation="MEKLPVNPNCKLSQTRYCQTLNMRSCSVCTVRDADDKDEIMKDI
                     DLYETLLPEGGIAQLFESRECQFCKPPQKGTRSGYAILDMAHPEPRRVQKWLFGKRTA
                     RIGTMVPVQISVCKKCRSRFLALEYLPILIPVILGLIALFAVSADPLKTVLADIHLFL
                     PFGVWLGCVLIGALAGKLITDALAKSWNKDMVVDVMQHPVIAAMTEKGWVPITAKSRT
                     KLLFSKTRLNKGLGTADHWGEDEETV"
     gene            1657..>1895
                     /gene="rpsL"
                     /locus_tag="PLR57_05990"
     CDS             1657..>1895
                     /gene="rpsL"
                     /locus_tag="PLR57_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HQQ41044.1"
                     /translation="MPTINQLVRKGREQVEYKSTAPILKQCPQRRGVCTAVRTMTPKK
                     PNSALRKIARIRLTDGLEGTAYIPGIGHNLQEHSVV"
BASE COUNT          446 a          548 c          540 g          361 t
ORIGIN      
        1 gcgcgtaagc ccaagggtct cgccgtcatc agcgagatca acggcgtggt gcacatcgac
       61 gacagcaaga agcgcaaaga ggcggttgtc accaacgagg acggagaagc ggaaagctac
      121 ctcatcccgt tcggcagcaa gctcaaggtt agcgacggcg atgttgtgga agcgggcgac
      181 gaactcacgg aaggctccgt caacccgcac gacatcttaa agatcaaggg cgtcaagggc
      241 gttcaggctt acctgctcaa ggaagtccag agcgtttacc gcctccaggg cgttgagatt
      301 tcggataagc atatcgaagt catcatccgc cagatgctcc gcaaggtgca ggtcgaggag
      361 aacggcgaaa ccacgatgct tccgggcgag ctcatcgaca tcttccgctt tgaagaggag
      421 aacgagcgtg tcgtccagaa cgacggcaag cccgctattg caaagcgcaa gctgctgggt
      481 atcaccaagg cctcgctggc gacggactcg ttcctctccg ccgcgtcctt ccaggagacc
      541 acgcgcgttc tgaccgaagc cgccatcaag ggcaagatcg atccgctggt cggcctcaag
      601 gaaaacgtca tcatcggcaa gctgatcccc gcgggcatcg gcctgcgccg gtaccgcaac
      661 gtcaacgtaa ccaccaagga ctgagcgaag gataccgcac cgcccgaaag gagccgaaat
      721 ggaaaaactg ccggtcaacc cgaattgtaa actctcccaa acgcgctatt gccagacgct
      781 gaacatgcgt tcctgcagcg tttgcaccgt ccgcgacgcg gacgataagg acgagatcat
      841 gaaggatatc gacctgtatg aaacactcct gccggaaggc ggcatcgcgc agctctttga
      901 gtcccgcgag tgccagttct gcaagccgcc tcaaaaaggc acccgcagcg ggtacgccat
      961 actcgatatg gcgcatcccg aaccgcggcg tgtgcagaaa tggctcttcg gcaagcgcac
     1021 ggcgcgcatc ggcactatgg ttcccgtgca gatttcggtc tgcaagaagt gccgctcgcg
     1081 gtttctggcg cttgagtacc tgccgattct gattccggtt attctcggcc tgattgcgtt
     1141 gtttgccgtt tcggccgacc cgctcaagac tgtgctggcg gatatccacc tgtttctgcc
     1201 gttcggcgtc tggctcgggt gcgtgctcat cggcgcgctg gccggaaagc tcatcaccga
     1261 cgctctggca aagagctgga acaaggatat ggttgtggac gtcatgcagc atcccgtcat
     1321 cgccgcgatg accgaaaaag ggtgggttcc gatcaccgcc aagtccagaa ccaagctgct
     1381 cttcagcaaa acccgcctga acaaaggcct cggcacggcg gatcactggg gagaagacga
     1441 agaaaccgtt tgacaaaagc gggtttttgc tgatacaata ccattttggc ggttcctgtt
     1501 tccgcgcatg tgcgcaaggt gttgcgccgc caagggtttt acacgctttt agaagtcttt
     1561 ttacagattt ttaaaagttc tgggatacaa tgctccctac aggggttgca gggagcggta
     1621 atagatatag atgaaaaata tcgaaggaga atattcatgc cgacaatcaa ccagttggtt
     1681 cgaaaaggca gagaacaggt ggaatacaag tccactgcgc ctattttgaa acagtgcccg
     1741 cagcgccgcg gcgtttgcac ggctgtccgt accatgacgc cgaaaaagcc gaactccgcc
     1801 ctgcgtaaaa tcgcccgtat ccgtctgacc gacggtcttg agggcaccgc ctacatcccc
     1861 ggcatcggcc acaacctgca ggagcactcc gttgt
//
LOCUS       DAOMGY010000355         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_70591_length_1890_cov_2.641417, whole genome shotgun sequence.
ACCESSION   DAOMGY010000355 DAOMGY010000000
VERSION     DAOMGY010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70591_length_1890_cov_2.641417"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLR57_05995"
     CDS             <1..242
                     /locus_tag="PLR57_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41045.1"
                     /translation="CGLDKAVQSTSESGLDFTDKLNPDSLVTLHGFVEPALANAPIGE
                     HYQFLREGYFCTDLDSKPGAPVFNRTVGLKDSYKG"
     gene            337..1772
                     /gene="gltX"
                     /locus_tag="PLR57_06000"
                     /pseudo
     CDS             337..1772
                     /gene="gltX"
                     /locus_tag="PLR57_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
BASE COUNT          466 a          523 c          532 g          369 t
ORIGIN      
        1 agtgcgggct cgacaaagcc gtgcagagta ccagcgagtc cgggttggac tttaccgaca
       61 agctcaaccc ggactcgctg gttactctgc acggctttgt cgagcccgca ctggcaaatg
      121 cgcctatcgg cgagcactac cagttcctgc gcgaagggta tttctgcacc gacctcgatt
      181 ccaagcccgg tgcgccggtc tttaacagga cggtcggctt gaaagacagc tataaaggtt
      241 gatttctttt ctttttaaaa aaagaaaaga aacaaaagaa aaattctttt gcttactttt
      301 cttataagaa aagtaaggga aattaggaga aacccaatgg ttcgaacccg gtttgccccg
      361 tcgccgacgg gatatatgca cctcggcaac ctgcgcagcg cgctgtatac ctacctttac
      421 gccaaaaagc acggcggcaa attcatcctg cgcattgagg ataccgacca gagccgttat
      481 gtcgaaggcg cggtggacgt gatcttcaac accctgcgcg atatcggcat gcagtgggac
      541 gaaggcccgg acgtcggcgg ggattatggc ccgtacgtcc agagcgaacg caagagcatg
      601 tatctgccct atgccgagca gcttgtgcgc gagggaaagg cctactactg cttctgcacc
      661 aatgaggaac tggacgcgcg gcgcgaagcc gcttccgccg agggcgaagt ctggaaatac
      721 gacaagcact gcctgcacct gccaaaggat gaaattcagc ggagaatcgc tgcgggcgag
      781 ccgcacgtca tccgccagaa cgttccgctc acgggcgaag cgtcgtttga cgacgtgctc
      841 tacgggcata tcgccgtgaa ctgcgcggat ctggacgata tgatcctgat caaggccgac
      901 ggcatgccta cgtataactt tgccaacgtg atcgacgacc acactatggc cattacgcac
      961 gtcatgcgcg gcaacgaata cctcgccagc acgccaagta caacctgctc tacgaggcgt
     1021 tcggctggga aaaacccgtg tatatccaca tgacgccgat catgcgggac gcgacccata
     1081 agctcagcaa gcgcgacggc gacgcgtatt acgaggatta cctcaaaaaa ggatacctca
     1141 aggaagcgat cgtcaactac gtcgccctgc tcggctggaa tcccggcggc gagcgggagt
     1201 tctttacgct ggatgagctc tgcgaagcgt tcgacgtgga cggcatgtcc aagtccccgg
     1261 cgattttcga cgtcaacaaa ctcacctgga tgaacgcgga gtatgttcgc cgcctgaccc
     1321 cggaagagtt tacccggcac gccatgccgt attacgagca ggcgaatgta gcgagcatgg
     1381 atcacggcat tctctgccgc atcctgcagc agcgcacgga ggtgtttacg cagattccgg
     1441 agatggtgga ctttttgacc gttctgccgg aatacgacgc ggaactcttt accaacaaaa
     1501 agagcaagac ggacgccgaa gtttccgcgc aggtgctcga tatggcaatc cccgcgctgg
     1561 aagcgctgcc taactggacg gaggaagcgc tgcacgccac gcttctcggc cttgccgagc
     1621 aaaacggcat gaagaacggc accatgctct ggccggtgcg catcgcaatg gcgggcaagg
     1681 ccgtgacccc cggcggcgca atcgagatcg ggctgctgct cggacgcgaa gaaacgctca
     1741 gaaggctgaa gttcgggcgg gagaaactat aacgatttct ttcttttaaa aaagaaagaa
     1801 acaaagaaaa gtatttcttt tgctgctttt ctttttaaga aaagcagaag gaggttcacc
     1861 ctttatgaaa cttggagtga tcggcctgcc
//
LOCUS       DAOMGY010000356         1889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_70620_length_1889_cov_3.893130, whole genome shotgun sequence.
ACCESSION   DAOMGY010000356 DAOMGY010000000
VERSION     DAOMGY010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70620_length_1889_cov_3.893130"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLR57_06005"
     CDS             complement(<1..617)
                     /locus_tag="PLR57_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010678014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQQ41046.1"
                     /translation="MEQSYADVNAKAVDRWVEDGWEWGVPVSHEAFLAAKQGDWSVVL
                     TPTKPVPKEWFPDLRGLEVLGLASGGGQQMPLFTAAGARCTVLDYSEKQLESERMVAE
                     REGYDINIVRADMSKPLPFGDNSFDVIFHPVSNCYIREVEPVWRECFRILRPGGVLLC
                     GLDNGLNYIFDDATESVLTRKLPFDPLTDAALYEELYGWHSQGGH"
     gene            796..1707
                     /locus_tag="PLR57_06010"
     CDS             796..1707
                     /locus_tag="PLR57_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQQ41047.1"
                     /translation="MPDIAIIGGGPAGLTAGLYAARGGADVVLYEELFPGGQIARTPM
                     VENYPGFPDGIEGYAVGGKIQKQAEKFGLKIEYAGATKLVLDVPDKQIILPDRVDTAK
                     AVILCMGATARPLGIPREEELTGAGVSYCATCDGAFFKDMPVAVVGGGDTALSDALYL
                     SRFCKSVTVVHRRDEFRGSVSLQNAAREKENISFALSCVPVSFVADKKVEGLEIRDLK
                     TNLTRVIPANGVFVAVGITPRTELVKDIVALTEGGSIITDEFMRTNLPGVYAAGDIRN
                     TPLRQVITACADGAIAGTTALEFGCSH"
     gene            1751..>1889
                     /locus_tag="PLR57_06015"
     CDS             1751..>1889
                     /locus_tag="PLR57_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQQ41048.1"
                     /translation="MGRLFGTDGVRGIANRDLSCELAYQIGAVGAYVLANEVHSPRIL
                     IG"
BASE COUNT          402 a          575 c          542 g          370 t
ORIGIN      
        1 tagtgccccc cctggctgtg ccatccgtac agctcctcgt agagcgccgc atccgtaagc
       61 gggtcaaagg ggagctttcg cgttaagacc gattccgtcg cgtcgtcgaa aatatagttc
      121 agcccgttgt cgagcccgca gaggagcacg ccgccgggac gcaggatgcg gaaacactcg
      181 cgccagaccg gttcgacctc gcggatgtag cagttggaaa cggggtgaaa gatcacgtcg
      241 aacgagttgt cgccaaacgg cagcggcttg gacatatccg cgcgcacgat gttgatgtcg
      301 tacccttccc gctcggcaac catacgctcg ctttcgagct gcttttcaga ataatccagc
      361 acggtgcagc gtgcgcctgc cgcggtaaag agcggcatct gctggccgcc gccgctggca
      421 agacccaaca cttcgagccc cctgagatcg gggaaccact ccttcggcac aggcttggtc
      481 ggcgttaaaa cgacgctcca gtcgccctgt tttgcggcaa gaaacgcttc gtgcgaaacg
      541 ggcacgcccc actcccagcc gtcttcgacc cagcggtcaa ccgctttggc gtttacgtct
      601 gcataagatt gttccatatg atttccctcc ccgggcggca cagtacggaa tttctcgccg
      661 cagggatagt atcgcatacc gtaaccatac ggcgcaagcg tgttttttgc gtgaagacaa
      721 agccccgttc gttgcatttt accgcgtatt tgggtatact gttactactt tcaatagact
      781 cggaggtaat atgtcatgcc ggatatcgca attatcggtg gaggccccgc cggactaact
      841 gccggactgt atgccgcgcg cggcggagcg gacgttgttt tatacgagga gctctttccc
      901 ggcggacaga tcgccagaac accaatggta gaaaactacc ccggattccc ggacggaatc
      961 gagggttacg ccgtcggcgg caaaattcaa aagcaagccg aaaagttcgg cctcaagatt
     1021 gaatacgcgg gcgcgacaaa actcgtgctc gacgttcccg acaaacagat catcctgccc
     1081 gaccgcgtgg acaccgccaa ggccgtcatc ctctgcatgg gtgccaccgc gcgtccgctc
     1141 ggcattccgc gcgaggagga actcaccggc gcgggggtaa gctactgcgc cacctgcgac
     1201 ggagcgttct ttaaggacat gcccgttgcg gtcgtcggcg gcggggacac cgcgctttcg
     1261 gacgcgctgt acctttcgcg cttctgcaag agcgtaacgg tcgtacaccg cagagatgag
     1321 ttccgcggtt ccgtttcgct ccagaacgcg gcgcgggaaa aagagaacat ctcgtttgcg
     1381 ctctcctgcg tgccggtatc cttcgttgcc gataaaaagg tggaaggcct cgaaatccgc
     1441 gacctgaaga ccaatctgac ccgcgtgatt ccggcaaacg gcgtattcgt cgccgtcggc
     1501 attacgccgc gcacggagct ggtcaaggat atcgtagctc tcacggaagg cggcagtatc
     1561 atcaccgacg aatttatgcg cacaaacctc cccggcgttt acgccgcggg cgatatccgc
     1621 aacacgccgc ttcgtcaggt catcaccgcc tgcgcggatg gcgcaatcgc gggcacaacc
     1681 gcgctggaat tcggctgctc ccactaagcg caatccaacg caagaaacga cgattaggag
     1741 aaagtaatcc atgggtagat tattcggaac ggacggcgta cgcggtattg caaaccgcga
     1801 tttaagctgc gaactggcct atcaaatcgg cgcggtcggc gcgtatgttt tggcaaacga
     1861 ggtgcattcc ccccgcatcc tgatcggca
//
LOCUS       DAOMGY010000357         1888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_70676_length_1888_cov_3.679760, whole genome shotgun sequence.
ACCESSION   DAOMGY010000357 DAOMGY010000000
VERSION     DAOMGY010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70676_length_1888_cov_3.679760"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLR57_06020"
     CDS             complement(<1..181)
                     /locus_tag="PLR57_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQQ41049.1"
                     /translation="MKTKANRDIWSVMKIVILVFVALAILYPLSTIVIRSVTTEAGEV
                     TGKNFLKFFTKPYYLD"
     gene            complement(199..1281)
                     /locus_tag="PLR57_06025"
     CDS             complement(199..1281)
                     /locus_tag="PLR57_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ41050.1"
                     /translation="MSHQVNIQNVVKRFGSFAAVDDVSLEIRPGEFFTLLGPSGCGKT
                     TLLRAIAGFNQPDGGEIRFSGKVINRIPAHKRDIGMVFQNYAIFPHMTVRENVAYGLK
                     ARNYPKNRIDGKVEEALEMVQIAPLRDRKPSELSGGQQQRVALARAFIIEPGVLLMDE
                     PLSNLDAKLRVQMRATIKKLQRRLGITTIYVTHDQEEALAISDRIAVMRDGKIMQLGT
                     PEEIYKHPKNRFVCGFIGVSNFVDCEAHTDASGAAHLEMGGYSAETRVLRPLEGKAVV
                     SARPEQLFFGETGIPGTIALSTFLGDFIEYEVLTRDGQTLLINEYTKDVAGVRPDGEA
                     CHIRFDPERIALIDPETEEVVSAFGQ"
     gene            complement(1381..>1888)
                     /locus_tag="PLR57_06030"
     CDS             complement(1381..>1888)
                     /locus_tag="PLR57_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQQ41051.1"
                     /translation="LCNMILSESKQADYSDGWAFIEQLAPNLIISGGSSSVYKGVAAG
                     EYALGLTLEQLAWVFVGADPDTCGMIYPEDGSSNVPDGFAMVANCPNPDMAALLMDFL
                     VSAECQQVMSDEFGRRTVRDDIAQPAALPNLDDIYFINYDFQWAGAGKADVVSHWEEI
                     AVNVISAG"
BASE COUNT          363 a          572 c          555 g          398 t
ORIGIN      
        1 catccagata atagggcttg gtgaagaatt tgaggaagtt ctttcctgtg acctcgcccg
       61 cctccgtggt cacgctgcgg atgacgattg tcgagagcgg gtagagaatt gccaacgcga
      121 caaagaccag aatgacgatc ttcatcacgc tccagatatc gcggttcgcc ttcgttttca
      181 tacgctttct ccccgctttc actgcccgaa tgcggagacg acttcctccg tttcggggtc
      241 gatcagggcg atgcgttccg ggtcgaagcg aatatggcag gcttcgccgt ccggccgcac
      301 gcccgcgacg tccttggtgt attcgttgat caagagcgtc tgcccgtcgc gcgtgagaac
      361 ctcatactcg atgaaatcgc ctaaaaacgt gctcagcgct atcgtgcccg gtatgccggt
      421 ttcgccaaag aagagctgct cggggcgcgc ggaaaccacc gccttgccct ccagcggacg
      481 gagcacgcgc gtttcggcgg agtatccgcc catctccaga tgcgccgcgc cggaagcgtc
      541 cgtatgcgcc tcgcagtcga cgaagttcga aactccgata aacccgcaaa cgaagcggtt
      601 cttcgggtgt ttgtagatct cctccggcgt gccgagctgc atgattttac cgtcccgcat
      661 gaccgcgatg cggtccgaaa tggcgagcgc ttcctcctga tcgtgggtca cgtagatggt
      721 cgtaatgccc agcctgcgct ggagcttttt aatcgtagcg cgcatctgca cgcggagctt
      781 tgcgtccagg ttggagagcg gctcgtccat gagcagcacg cccggttcta tgataaacgc
      841 gcgcgccaga gcgacgcgct gctgctgtcc gccggataat tcggaaggct tgcggtcgcg
      901 cagcggcgca atctgcacca tttccagcgc ttcttcgacc ttaccgtcga tcctgttctt
      961 gggataattc cgtgctttca gcccgtaggc tacgttttcc ctgacggtca tatgcggaaa
     1021 gatggcatag ttttgaaaca ccatgccgat atcccgctta tgcgccggaa tgcggttgat
     1081 caccttgccg gaaaaacgaa tctcgccgcc gtccggctgg ttgaatccgg caatggcgcg
     1141 tagcagcgtc gtctttccgc agccggaggg gccgagcagc gtaaaaaact cgcccggacg
     1201 aatctccagc gaaacgtcgt ccacagcggc aaagctgccg aagcgcttga cgacgttttg
     1261 aatgttaact tgatggctca tgtggttacc cccgtatctt ttcccggata tcaggcaggc
     1321 gcccggccga agccgaagcg cccgccttgt gaatcagtcc gattccgttt tgccgctttc
     1381 tcagccggcg gaaatcacgt tgacggcgat ctcttcccaa tgggacacga cgtcggcttt
     1441 gccggcgccc gcccactgga agtcgtagtt gatgaagtag atatcgtcca gattcggcag
     1501 cgcggcgggc tgggcgatgt cgtcacgcac ggtacggcgg ccgaactcgt cgctcatgac
     1561 ctgctggcac tctgcgctca cgaggaagtc catcagcagc gccgccatgt ccggattcgg
     1621 gcagttggca accattgcaa agccgtccgg cacgttggag gagccgtcct ccgggtagat
     1681 catgccgcag gtgtcgggat ccgcgcccac gaacacccag gcaagctgtt ccagcgtcag
     1741 gccgagcgcg tattcgcccg cggcaacgcc cttgtaaacg gaggaagagc cgccggagat
     1801 tatgaggttc ggcgcgagct gttcaataaa cgcccagccg tcggaatagt ccgcctgctt
     1861 ggattccgag agaatcatgt tgcaaagc
//
LOCUS       DAOMGY010000358         1886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_70796_length_1886_cov_3.486619, whole genome shotgun sequence.
ACCESSION   DAOMGY010000358 DAOMGY010000000
VERSION     DAOMGY010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70796_length_1886_cov_3.486619"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLR57_06035"
     CDS             <1..188
                     /locus_tag="PLR57_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41052.1"
                     /translation="KNQLVFLEAIVAPAESGSLSAGVKRIFSANLNFTPDCRTMTVFQ
                     LTKRVIAAAAQQRLIMI"
     gene            214..>1886
                     /locus_tag="PLR57_06040"
     CDS             214..>1886
                     /locus_tag="PLR57_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41053.1"
                     /translation="MAKRRKKKRFRMQPRFFLVMVAILGAIILILLLVKKVGKDSEDA
                     LLATPVPTATLAPTATPKPALPEDVTVTRADSANPSTYGFKGTISFNGNDVSSYSREN
                     KIFFGRNIEYASIPGVLTFGGNNYRDSFAYGTASISEKRLRQQWAKTIGSLGTWSGTG
                     WTGQPLIVQWPAETVAVMNVAQSYKDANAPLTEVIYPAMDGYIYFLDLNTGAATRDPI
                     TTGIVQKGTACVDPRGYPLLFVGQGIAVENDEGNNSAYVRVYSLITGEEIYKFGGFDW
                     FSRRKWQAYDGSPMISDDTIVYGGENGVLYTSKLNATFDIAAGTVTIDPERLVKMRYE
                     GTGYSREDMTGSRWYGMESSVAAFRNYAYTTDNGGRLQCVDLNTMQIKFVVDLTDETD
                     STPVIEESYDDNTIYLYTGSQVRSSNGDLGDGYGYSYQRKINGLTGAVVWEQKWPCSI
                     GDANASGGIVSTPHVGKGRISNLVIYSFSLAALSNGSAAAADTPTPETTDEAAASTAA
                     VMPQPDANGAYTLGGRIVALDKSTGAVVWSIEQSNDYWAQIEAGAVRST"
BASE COUNT          436 a          527 c          544 g          379 t
ORIGIN      
        1 gtaaaaatca gctagtattt cttgaagcaa tcgttgcccc ggcggagagc ggaagccttt
       61 ccgccggggt gaaacgcatt ttttcggcga atttgaattt cacgcctgat tgtcgtacaa
      121 tgacagtatt ccagctcacg aaacgtgtca ttgcggcagc cgcacaacaa cgtttgatta
      181 tgatttgatt ataaatagta aggagggagc acggtggcaa aacggcgcaa gaaaaaacgg
      241 tttcgcatgc agccgcgatt ctttcttgtc atggtcgcga ttcttggcgc gatcattctg
      301 atattattgc tggtcaagaa ggtagggaag gacagcgagg acgccctgct ggctacgccc
      361 gtgccgacgg cgacgctcgc gccgaccgca acgcccaagc ccgccttgcc ggaggacgtt
      421 acagtcaccc gcgcggacag cgcaaacccg agcacctacg ggttcaaggg tacgatttcg
      481 ttcaacggta acgatgtttc gagctactcg cgcgaaaaca agattttctt tggacggaac
      541 attgagtatg cgtcgattcc gggcgtactc acctttggcg gcaacaacta tcgcgattcg
      601 tttgcctacg gcacggcttc gatttcggaa aaacggctcc gccagcaatg ggcaaagacg
      661 attggctctc tgggaacttg gtccggtacc ggctggacgg gtcagccgct gattgtgcag
      721 tggcccgccg aaaccgtcgc ggttatgaac gttgcccaga gctataagga cgctaacgcg
      781 ccgctgaccg aagtgatcta tcctgcaatg gatggttaca tctattttct cgatctgaat
      841 accggcgcgg caacgcgcga tccgatcacc accggcatcg ttcaaaaggg aaccgcatgc
      901 gttgacccgc gcggttatcc gcttttgttc gttggccagg gcatcgccgt ggaaaacgac
      961 gaaggcaaca actccgccta tgtacgcgtc tattcgctga ttacaggcga agagatctac
     1021 aaattcggag gattcgactg gttcagccgc agaaaatggc aggcgtacga cggaagcccc
     1081 atgatttccg acgataccat tgtatacggc ggtgaaaacg gcgttttgta tacgtccaaa
     1141 ctcaacgcga cgttcgatat tgccgcggga acggttacca tcgatcccga acggctcgtg
     1201 aagatgcgct acgaaggcac gggctattcc cgcgaggaca tgaccggaag ccgctggtac
     1261 ggtatggaga gctcggttgc cgcgtttaga aattacgcct acacaaccga taacggcggc
     1321 aggctgcagt gcgttgatct caacaccatg cagatcaagt tcgttgttga tctcaccgac
     1381 gaaacggaca gcacgcccgt aatcgaagag agttacgacg acaatacgat ttacctctac
     1441 accggttcgc aggtgcgctc cagcaacgga gacctcggcg acggatatgg atacagctat
     1501 cagagaaaga tcaacggact gaccggcgct gtggtctggg aacagaagtg gccctgctcc
     1561 attggggacg ccaacgccag cggcggcatc gtttccaccc cgcatgtcgg caaagggcgc
     1621 atttccaacc tcgtgatcta ttccttcagc ctggccgcgt tgtccaacgg ttctgcggct
     1681 gcagcggaca cgcccacgcc ggagacaacg gacgaagcgg ccgcttccac cgccgccgtc
     1741 atgccccagc cggacgccaa cggcgcgtac acgctgggcg ggcgcatcgt ggcgctggat
     1801 aagtcgaccg gcgcggtggt ctggagcatt gagcagtcaa acgactactg ggcgcagata
     1861 gaggccggag ccgtccgctc gacgat
//
LOCUS       DAOMGY010000359         1883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_70965_length_1883_cov_4.157549, whole genome shotgun sequence.
ACCESSION   DAOMGY010000359 DAOMGY010000000
VERSION     DAOMGY010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70965_length_1883_cov_4.157549"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PLR57_06045"
     CDS             complement(<1..739)
                     /locus_tag="PLR57_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQQ41054.1"
                     /translation="MSRFMDETALYYFNRGVSTAAYRELGCHEETGEFGERVYRFAVW
                     APNAEFVSVVGDFNFWNVEADRMERAGTTGVWELRIGIANEGDLYKYAIGTYTGEILM
                     KADPFAFRCEARGTASIVGDMPEFDWTDEEYLSSRAGSFASLDQPMSIYEVHLGSWKM
                     GLGYRELADELVDYVADMGYTHIELMPLMAYPYDPSWGYQVTGYYAATARYGEPEDLM
                     YFVDRAHELGIGVIMDWVPAHFTKDAHG"
     gene            1062..>1883
                     /locus_tag="PLR57_06050"
     CDS             1062..>1883
                     /locus_tag="PLR57_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HQQ41055.1"
                     /translation="MENLLLSFRVVAPLMLYIAVGALLRKTGIVDRPTLRGVNNIVFY
                     ASLPVLCFQKIAASDLSSMVETPFLLYIALAILLLFVLSSIIVPRVCKDDRRRGVLIL
                     GVFRSNDAIFGLGVAAALLGDDHLTLMTLAIALTVPLFNVLSVLAMERYRGGKVNIGQ
                     LLLRVIKNPIVLGCLAGFIANFTHLQMPEFLNQPLTGLAALCTPLAFVSLGGTIAFSA
                     LRKNALAITVVNLLRLIIIPVAVIGTFLLLGYRGEPIVVIMILFGAPVAIVTYSMA"
BASE COUNT          351 a          587 c          492 g          453 t
ORIGIN      
        1 ggccgtgcgc gtccttcgta aagtgcgcgg gcacccaatc catgatgacg ccgattccca
       61 gctcgtgcgc gcggtcgacg aagtacatga ggtcttccgg ctcgccgtag cgcgcggtgg
      121 ccgcgtaata ccccgtcacc tgatagcccc agctagggtc gtagggatac gccatcagcg
      181 gcatgagttc aatatgcgta taccccatgt ccgcaacgta atccaccagc tcgtccgcaa
      241 gttcccggta accaagcccc atcttccagc tgccgagatg cacttcgtaa atgctcatgg
      301 gctgatccag agacgcaaaa gaaccggcgc gggagcttaa gtactcctcg tcggtccagt
      361 caaactccgg catgtcccca acgatggacg cggttccgcg cgcttcgcag cgaaacgcga
      421 acggatcggc tttcatgagg atttcgcccg tataggtgcc aatggcatac ttgtagagat
      481 cgccttcgtt ggcgatgccg atgcggagtt cccaaacgcc ggtcgtcccg gcgcgttcca
      541 tgcggtcggc ttccacattc cagaagttga agtctcccac tacgctgacg aattccgcgt
      601 tcggcgccca aacggcgaaa cggtaaaccc gttcgccaaa ttcgcctgtt tcttcgtgac
      661 agcccagttc gcgataagcg gccgtcgata cacccctgtt gaagtaatag agtgccgttt
      721 catccatgaa tctgctcatc catgcaaccc ccgcgtttaa cgctacaatt agatgatacc
      781 atttttaccg cttaaaagga acagctatct gccgatttaa catcgccacg cttattgcgt
      841 gtgtcaattc cgcccgaatt aacggttttc tgccggttcc ggttttttca aagtcacgcg
      901 ctgtcgcctt gatatttctc tgacaatgag ttgatctgca cttcttctca gcattcgtgc
      961 ggatttccgc agagcggtgc gttttttcaa agttatggta taatagcgca tgttcgccgg
     1021 ttttccggcg ttcgaaatac aaaggaatcc ggagccgctc catggaaaat cttctgctct
     1081 cgtttcgcgt ggtcgcgccg ctgatgctct atattgcggt cggcgcgctg ctgcgcaaaa
     1141 ccggaattgt cgaccgtccg accctgcgcg gggtcaacaa catcgtgttc tacgcgtcgc
     1201 tgccggtgct gtgttttcaa aaaatcgccg caagcgatct ttccagcatg gtggaaacgc
     1261 cgtttctgct gtatatcgcg ctggccatcc tcctgctgtt tgtgctttca agcatcatcg
     1321 ttccgcgcgt ttgcaaggac gaccgcaggc gcggggttct gattctcggc gtgttccgct
     1381 ccaacgacgc catctttggc ctcggcgttg cggcggcgct gctgggcgac gaccacctga
     1441 cgctcatgac gctcgccatc gcgctgacgg taccgctgtt caacgtgctc tccgtgctcg
     1501 ccatggagcg ttaccgcggc ggcaaagtca acatcggtca actcctgctg cgagtcatca
     1561 aaaaccccat tgtgcttgga tgcctcgcag gttttatcgc aaatttcacc catctgcaga
     1621 tgccggagtt tctaaaccag ccgctcaccg ggctcgccgc cctctgcacg ccgctggcgt
     1681 tcgtttccct cggcggcacg attgcgtttt ccgccctgcg caagaacgcg ctggccatca
     1741 cggtggtcaa cctgctcagg ctgatcatca tccccgtcgc cgtgatcggc acgtttctgc
     1801 tgcttggcta ccgcggtgag ccgatcgtgg tcattatgat cctcttcggc gcgccggtcg
     1861 caattgtaac ctactccatg gcc
//
LOCUS       DAOMGY010000360         1882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_71025_length_1882_cov_3.175151, whole genome shotgun sequence.
ACCESSION   DAOMGY010000360 DAOMGY010000000
VERSION     DAOMGY010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71025_length_1882_cov_3.175151"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLR57_06055"
     CDS             <1..429
                     /locus_tag="PLR57_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HQQ41056.1"
                     /translation="DCAQKAQAQSGAGAYSDKDWLTTLLFSIFLGGLGIHRFYVGKIG
                     TGILWLFTAGVFGVGALVDIILIATESFTDDAGRAIVQDSKKIPNRAYAGTQSAYAGA
                     QSASSDVYEQLARLSSLRDSGAISQEEYDAKKAVLLEKIK"
     gene            complement(516..>1882)
                     /locus_tag="PLR57_06060"
     CDS             complement(516..>1882)
                     /locus_tag="PLR57_06060"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQQ41057.1"
                     /translation="RTIHLLTLLLNHKTGADQLDCGTITGFAGRPGAGRIEGEGMKKH
                     MLRSTTHFMRRTLFTLFLTLLLCAALLMTGCGRKKTTATSIGWQEQRICNNERLSDED
                     VIKQIAAFLLFVDKEEVITAKTLTFSDCVYILRTMLVHTDAIIDERVQFNDPVISVDA
                     DTANEYICLLFGCDMPPLDEYYVLPKGMSTDQEYGVRDGMFYFGTGWWNAGKYQYSDG
                     KTDSIDYNEDGTISADIVYHPRYWMDAHVIMTLIPVDTAFRFQIVSFQNVIVDNTVEE
                     PENEATPVQTEETPDPSACATPVDGRLTAAILAYDQADYEESFPLFWELADEGNAYAQ
                     YFVGESYKYGYGVEGSSEKCVYWYMTSAANGDGYGQASLGTCYRDGYGVGADYEKMLY
                     WYTLSAQQANPSGLINLGYCYHKGIGVEVNLEEARDLYELADLAGHPYAKTRLAEVNL
                     LLCG"
BASE COUNT          417 a          542 c          475 g          448 t
ORIGIN      
        1 gactgcgcgc agaaagcgca ggcgcagtcc ggcgcgggcg cgtactcgga taaggactgg
       61 ctcacgacgc tgctcttctc catctttctc ggcgggctcg gcatccaccg cttctacgtc
      121 ggcaagatcg gcacgggcat cctctggctc ttcacggcgg gcgtattcgg cgttggcgcg
      181 ctcgtggaca ttatcctgat tgcgaccgaa tcgttcacgg acgacgcagg ccgcgccatc
      241 gttcaggatt cgaagaagat tcccaatcgt gcttacgcgg gtacgcagag cgcctacgcg
      301 ggcgcgcaga gcgcttccag cgatgtgtac gaacaactcg cgcggctttc cagcctgcgt
      361 gactccggcg caatttcgca ggaagagtac gacgcgaaaa aggccgtgct gcttgagaag
      421 atcaaataat ttaggatagc gtttcaaacg aaatccccgt cattaccgac ggggattttt
      481 tttgcgcaat tcttattatt acaattcaat ttcgctcagc cgcaaagcag caagttcact
      541 tccgcaaggc gcgtctttgc gtacgggtgc cccgccaaat ccgccagctc gtaaaggtct
      601 ctcgcttctt ccagattgac ttctacgcca attcctttat ggtagcagta cccaaggttg
      661 atcaggccgg aaggattcgc ctgctgggca gaaagcgtgt accagtaaag catcttttca
      721 taatcagcgc ctacgccgta cccgtcgcga tagcacgtac ccaggctggc ctgaccgtac
      781 ccgtcgccat ttgccgccga agtcatgtac cagtaaacgc atttttcgga gcttccttca
      841 acgccgtaac cgtacttata cgactctcca acaaagtact gcgcgtaagc gttgccttca
      901 tccgcaagct cccaaaagag cggaaacgat tcttcgtagt ccgcctggtc atatgcgagg
      961 attgccgccg tcaatcgccc gtcaacaggc gtcgcgcagg cgctcggatc gggcgtttct
     1021 tccgtttgaa cgggagtcgc ttcgttttcc ggttcctcaa ccgtattatc gacgattaca
     1081 ttttgaaacg aaacgatctg aaagcggaaa gccgtatcga cgggaatcag cgtcataatt
     1141 acatgcgcgt ccatccagta ccgcggatga tacacaatat ccgcgctgat ggttccgtcc
     1201 tcgttgtagt cgatgctgtc cgtttttccg tcactgtatt gatacttgcc cgcgttccac
     1261 cagcccgtgc cgaaatagaa catgccgtcg cgaacgccgt attcctgatc ggtagacatt
     1321 ccctttggca ggacgtaata ttcatcaagc ggaggcatat cgcacccgaa aagcaggcag
     1381 atatactcat ttgccgtatc agcatcaacg gagatcaccg ggtcgttgaa ctgcacgcgc
     1441 tcgtcaataa tcgcatccgt gtgcaccaac atggtgcgca atatgtagac gcagtctgaa
     1501 aaggtcagcg tttttgccgt gataacttcc tctttatcca cgaaaaggag gaaagcggcg
     1561 atctgcttga taacgtcctc gtcagacagc ctttcgttgt tgcagattct ctgctcctgc
     1621 cagccaatgc tcgttgcggt ggtctttttg cggccgcagc cggtcatcaa aagcgccgcg
     1681 caaagcagca gcgtcaagaa aagcgtaaac aacgttctgc gcataaaatg agtcgtcgaa
     1741 cgaagcatgt gttttttcat cccttccccc tcaatccttc ctgcgccggg tcgtccggca
     1801 aatccggtta tcgtgccgca gtcaagctga tctgcccccg ttttatggtt cagcagcaat
     1861 gttagtaaat gaattgttct aa
//
LOCUS       DAOMGY010000361         1878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_71242_length_1878_cov_3.273176, whole genome shotgun sequence.
ACCESSION   DAOMGY010000361 DAOMGY010000000
VERSION     DAOMGY010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71242_length_1878_cov_3.273176"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PLR57_06065"
     CDS             complement(<1..841)
                     /locus_tag="PLR57_06065"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HQQ41058.1"
                     /translation="MKKFVLAPDSFKGSLSANEICEIWSGAIKAHLPEAEIVRFPVAD
                     GGEGLVDAFLRVCGGERLTAMVQNPLGETVQAAYGILPNGSAVIEMAAAAGLPMIAER
                     KNPLIASTYGVGQLLLDAEQRGVKQVILGLGGSATNDCGVGMASALGYEFFGAAGNRV
                     EPLAKNMGKIARIQPPARRPALSVVAACDVDNPLYGPNGATYTYGAQKGATPELQAEL
                     DAGLINMARVIERDLGARVAEVPGAGAAGGLGAGVLAFLGGTLQPGIELLLDEAGFDG
                     MLEG"
     gene            complement(1017..1649)
                     /locus_tag="PLR57_06070"
     CDS             complement(1017..1649)
                     /locus_tag="PLR57_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQQ41059.1"
                     /translation="MSGARTLKWMADGGVNPLDQSPALYEATLTEFAEHSFRDASLNE
                     ILKAAGMNKGSFYYRFEDKMELYLSLLYRVGMEKLKLFEEEGVSRTSGGFFDEFRNMA
                     ILGLKLAKKDARFTVFSRRIMEEAPAVREAITACFGNLSGSLLTDMVERAKASGEFSA
                     DLSTETAVFVIETLLNHMDALIPAEWDDASVLAAVDRLLDVVRNGVGAKP"
     gene            complement(1649..1786)
                     /locus_tag="PLR57_06075"
     CDS             complement(1649..1786)
                     /locus_tag="PLR57_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41060.1"
                     /translation="MAWYWWALIIVGVVAIVALKVVFVPKYFKKQREKKAERERMEDE
                     D"
BASE COUNT          365 a          640 c          480 g          393 t
ORIGIN      
        1 caccttccag cattccgtcg aaccccgctt cgtcgagcag cagttcaatg cccggctgca
       61 gagttccccc cagaaacgcc agcacgcccg cgcctagccc gcccgccgcg cccgcgccgg
      121 gaacttccgc aacacgcgcg ccgaggtcgc gctcgatcac gcgcgccata ttgatcagcc
      181 ccgcgtcgag ctccgcctgc aattcgggag tcgcgccctt ctgcgcgccg taggtgtagg
      241 tggctccgtt cgggccgtag agcgggttgt ccacgtcgca ggcggcgacc acgctgagcg
      301 cgggtctccg cgcgggcggc tgaatccgtg caatcttacc catgttcttt gccagcggtt
      361 caaccctgtt tcccgccgcg ccgaaaaact cgtatcccag cgcgctcgcc atgccgacgc
      421 cgcagtcgtt cgttgcgctg ccgccaagac ccaaaatgac ctgctttacc ccgcgctgct
      481 ccgcatcaag caatagctgg cctacgccat aggtcgaggc aatcagcgga ttcttccgct
      541 cggcaatcat gggcagtccg gccgccgccg ccatctcgat gaccgcggaa ccgttgggca
      601 ggatgccgta cgccgcctga acggtttcgc ccagcgggtt ctgcaccatg gcggtaagcc
      661 gttcgccgcc gcagacgcgc agaaacgcgt ccacgagccc ttccccgccg tccgcaacgg
      721 gaaagcggac gatctccgct tccggaaggt gcgctttaat cgccccgctc cagatttcgc
      781 agatttcgtt tgccgaaagg ctccccttaa acgaatccgg cgctaacaca aacttcttca
      841 tcggctgacc ccgcttatca ctttgttcct atactcttcc taaaatagta tgtccgcagg
      901 cacgcgctgt caagcgcggg tactcttctg cgcgcgcaag tattttcgaa acaaaaatac
      961 ccggacgctc ttaatttatg agcatccggg taaatacaat gtggattctt gggaatttac
     1021 ggctttgcgc caacgccgtt tcgcacaacg tccagcagcc ggtctacggc ggccagcacg
     1081 ctcgcgtcgt cccactccgc cggaatcagc gcgtccatat ggttgagcag ggtttctatg
     1141 acaaacaccg ccgtctccgt ggataagtcc gcgctgaact cgccggaagc cttggcgcgt
     1201 tctaccatgt ccgtcagcag cgacccgctg aggttgccga agcaggcagt tatcgcctcc
     1261 cgcacggcgg gcgcttcttc catgatgcgg cgggaaaaca cggtgaagcg cgcgtctttt
     1321 ttcgcgagct tcaggccgag gatcgccatg ttcctaaact cgtcgaaaaa cccgccggac
     1381 gtcctggata cgccctcctc ctcaaagagt ttgagttttt ccatgcctac gcggtacaga
     1441 agcgaaagat acagctccat tttatcttcg aaccggtaat agaagctgcc cttgttcatc
     1501 cccgccgctt tgaggatttc gttgagcgaa gcgtcccgaa aggaatgctc tgcaaactcc
     1561 gtcagcgtcg cttcatacag cgcaggcgac tgatccagcg ggttgacacc gccgtcggcc
     1621 atccacttga gggttcttgc gccgctcatc agtcctcatc ctccatgcgt tcgcgttccg
     1681 cctttttctc gcgctgcttt ttgaagtact tcggtacgaa cacaaccttc agcgccacga
     1741 tggcgactac gccgacaata atcagcgccc accaatacca tgccattctt cttcacctcc
     1801 ccattctgaa tttgactttt ttcgcaaagt gccaaaccga cgccataaac cagggaatgc
     1861 tcaccaacag cgtcaaaa
//
LOCUS       DAOMGY010000362         1876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_71367_length_1876_cov_3.537068, whole genome shotgun sequence.
ACCESSION   DAOMGY010000362 DAOMGY010000000
VERSION     DAOMGY010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71367_length_1876_cov_3.537068"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1509
                     /gene="ortB"
                     /locus_tag="PLR57_06080"
     CDS             103..1509
                     /gene="ortB"
                     /locus_tag="PLR57_06080"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HQQ41061.1"
                     /translation="MTRDTSYEAVIARKRDIIKAAVGVDYSQFESGSIAFDYEAMMRS
                     TGYSLEDVIEIQRGFAIGNTPLIELKNITALARKLSKPGYGARIFVKDEAANPSGSFK
                     VRRAATSIYQARKMGYPGALTATSGNYGAAVASCCAKSGLKCIIVQECYDSRGVGQPE
                     IIEKARKCEALGAEVVQLTVGPELFYQVLRMLEDTGYFNASLYTAFGIVGIETLGWEV
                     AQQCRAQIGKDPDVVVCTNAGGGNLTGTARGLIKAGCKAQIVAASVNLKGLHMASDRQ
                     FNKKSFTTGHTGFGMPFSTWPDRSDVPRSAARPLRYMDRYVTVHQGEVFYTTELLARL
                     EGIERGPAGNTSLAAAISIAQELPEDAVIVVQETEYTGAGKHGNAQLSFARQNGIRIY
                     FGDPEEEVPGESIVLPANPGLLKARDVDIDHMRRSLITHAASTVEHAPTSADIAFLAE
                     ETKSSEAFVTETLKAEQH"
     gene            1546..>1876
                     /locus_tag="PLR57_06085"
     CDS             1546..>1876
                     /locus_tag="PLR57_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HQQ41062.1"
                     /translation="MKREDDYSKRREHLAALSDEELKARFWSLTEGLVDPLLQLGKEN
                     TSPAIERSVLLRMGFSSLEAKTIVDGCMARGILGHGAGNVVYRYATTQGLELRAAGLL
                     LQQPGYAV"
BASE COUNT          416 a          525 c          599 g          336 t
ORIGIN      
        1 cgaagtaaac ccgcattacg atcacagctt cggcggcttt gtgccggaac tgcagcaggc
       61 gggggacgag gcgtttgcct tcctgtttgg aggtgacgca aaatgacgcg ggatacgagc
      121 tacgaagcgg tcatcgcgcg gaaacgcgat atcatcaaag ccgcggtcgg cgtggactac
      181 tcgcagtttg agagcggctc cattgcgttc gactacgagg caatgatgcg ctccacgggc
      241 tattcgctcg aagacgtgat agaaattcag cgcgggtttg ccattggcaa cacgccgctg
      301 atcgaactaa agaacatcac cgcgttagcg cggaaactct cgaaaccggg ttacggtgcg
      361 cgcattttcg tcaaggacga agcggcaaac cccagcggaa gctttaaggt acgcagggcg
      421 gccaccagta tctatcaggc aagaaagatg ggctatcccg gcgcgctgac cgcgaccagc
      481 ggcaactacg gcgcggcggt tgcaagctgc tgcgctaaaa gcggtctcaa gtgcatcatc
      541 gtgcaggaat gctacgattc gcgcggcgtt ggccagccgg agatcattga aaaagcgcgc
      601 aagtgcgaag cccttggcgc ggaagtcgtc cagctcacgg tggggccgga gctgttttat
      661 caggttttac ggatgctgga ggacacgggg tatttcaacg cctcgctcta tacggcgttc
      721 ggcatcgtcg gcattgaaac gctcggctgg gaagtcgctc agcagtgccg cgcgcagatt
      781 ggcaaagacc cggacgtggt cgtctgcacc aacgctggcg gcggcaatct gaccggcacg
      841 gcgcgaggac tgattaaggc gggctgcaag gcgcagatcg tagcggccag cgtcaacctc
      901 aaaggcctgc atatggccag cgacaggcag ttcaacaaaa aatcgtttac cacgggtcat
      961 acaggctttg gtatgccgtt ttccacctgg ccggacagaa gcgacgtgcc cagaagcgcg
     1021 gcgcggccgc tccgatatat ggatcgctac gtcaccgtgc atcagggcga agttttttac
     1081 accaccgaac ttctggcgcg tctggaaggc attgagcgcg ggccagcggg caacacgagc
     1141 ctcgccgccg caatttcgat tgcgcaggaa ttgcccgagg acgcggtgat cgttgtgcag
     1201 gaaacggaat acaccggcgc gggtaagcac ggcaacgcac agctttcgtt tgcgcggcag
     1261 aacggcatcc gcatctactt cggcgacccg gaggaggaag tgcccggcga gagcatcgtg
     1321 ctgccggcaa atccgggcct gctcaaagcg cgggatgtgg atatcgacca catgcgccga
     1381 tcgctgatta cccatgcggc ctcgacggtc gagcacgcgc cgaccagtgc cgatattgca
     1441 tttctcgcgg aggaaacaaa gtccagcgag gcgtttgtaa cggaaacgct caaggccgaa
     1501 cagcattgat tcattccaca tttttaacga tatgggagcc gaattatgaa acgggaagat
     1561 gattattcaa agcgccgcga acatctggcc gcattgagcg acgaagagct caaagcgcgc
     1621 ttctggagcc tgacggaagg gctcgtcgat ccgctgctcc agctcggaaa agagaacacc
     1681 tcgccagcca tcgagcgcag cgtgctgctg cgtatggggt tcagttcgct ggaggcgaaa
     1741 accatcgtgg acggctgcat ggcgcgcggt attctgggtc acggcgcggg caacgtcgtt
     1801 taccgctacg caacaacgca ggggctggaa cttcgcgcgg ctgggctgct gctgcagcag
     1861 ccgggctacg cggtgc
//
LOCUS       DAOMGY010000363         1875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_71444_length_1875_cov_2.404396, whole genome shotgun sequence.
ACCESSION   DAOMGY010000363 DAOMGY010000000
VERSION     DAOMGY010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71444_length_1875_cov_2.404396"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /gene="uxaC"
                     /locus_tag="PLR57_06090"
     CDS             <1..1150
                     /gene="uxaC"
                     /locus_tag="PLR57_06090"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HQQ41063.1"
                     /translation="FKAFAGIMPELIGNPLYIWCALELKRYFGIGEPLSAENADEIYN
                     KTKALIAERNMCPRWCMEHSKVEMVCTTEDPTDTLQYHRLLKQEGLFRTKVISAFRPD
                     KAMNCEKAPFTGYMGDLEAAAGEKIGSFSELIAALEKRLLVFKSLGSMISDAGLDNFI
                     WREATERELDAILKKARRGEPLTREQVDQFRSAFLLQMGRVYARNGFVMQIHIGAYRN
                     ANGDMAKKLGPDTGYDCADSASDIRSVGALLDTLNREGNLPKVILYPLNASDVEPYAI
                     LAAAFCKGPGRGTVQLGAPWWFCDQVHGIQRQFEAVANLYPVALSVGMLTDSRSFLSY
                     PRHELYRRVFCNYLGELIERGEYFSGEKHLKTIVENVCYGNAKAYFGL"
     gene            1203..>1875
                     /locus_tag="PLR57_06095"
     CDS             1203..>1875
                     /locus_tag="PLR57_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HQQ41064.1"
                     /translation="MQTYPKSTHRTETCTVSMVRGKDADFLCVSGETPGFSGETADGE
                     FLAPLTHENAVLLRKLFPFTAPKPVLRELRSFGVGDRLGIATPGHLRVFEQYDAFPVL
                     AQQSIRELTLTKRTFADVLDSATFAVFREDFQKGFGADGDHLKTDEEVAYALANGYTM
                     ITLDCSEHIRNDVTGMTDEQVNAAYQPRPDWEKLYCGKTFAVEDQKVAFGEQEFRRAC
                     LIYAAA"
BASE COUNT          421 a          519 c          567 g          368 t
ORIGIN      
        1 gttcaaggcg tttgcgggca tcatgccgga actgatcggc aatccgctct atatttggtg
       61 cgcgctggag ctcaagcggt atttcggcat cggagaaccg ctttccgcgg aaaatgccga
      121 cgaaatctac aataagacca aagcgctcat tgccgagaga aacatgtgtc cgcgctggtg
      181 catggagcac tcgaaagtcg aaatggtctg caccacggag gaccctaccg acacgctgca
      241 gtaccatcgt ttactcaagc aggaagggtt gtttcgcacc aaggtgattt ccgcgttccg
      301 cccggataaa gcgatgaact gcgaaaaagc gccgtttacg ggctacatgg gcgacctgga
      361 agccgcagcc ggtgagaaaa tcggctcgtt tagcgaattg atcgcggcgc tggaaaaacg
      421 cctgctggta tttaagagcc ttggctctat gatcagcgat gcggggctgg ataactttat
      481 ctggcgtgaa gccacagagc gcgaactgga cgcaatcctg aagaaagcgc ggcgcggcga
      541 accgcttacg cgcgaacagg tcgatcaatt ccgcagcgcg tttctgctgc agatggggcg
      601 cgtttacgcg cgaaacggct ttgtgatgca gattcacatt ggcgcgtacc gcaacgccaa
      661 cggggatatg gccaagaaac tcggcccgga tacgggctac gattgcgcgg acagcgcaag
      721 cgacattcgc agcgtgggcg cgctgctcga cacgctgaat cgggaaggga acctgccgaa
      781 ggtcattctt tatccgctca acgcttcgga tgtggagcca tacgcaattc tcgcggcggc
      841 attctgcaaa ggcccgggtc gcggcacggt tcagctgggc gcgccctggt ggttttgcga
      901 tcaggtgcat ggcattcagc gtcagtttga agcggtggca aatctttacc ccgttgcgct
      961 ttccgtcggg atgctgaccg acagccgaag ctttttgagc tacccgcggc atgaactgta
     1021 ccgccgcgta ttctgtaatt atctgggcga gctgattgag cgcggcgagt atttctccgg
     1081 ggaaaagcac ttaaaaacca tcgttgaaaa cgtatgttac ggcaacgcga aggcgtactt
     1141 cggcctgtaa cgccgcataa agacaaattt aacaacctcg aatcaacagt aaggagataa
     1201 acatgcagac atacccgaaa tccacgcatc gcacagagac gtgcacggtt tccatggttc
     1261 gcggcaagga cgcggacttt ctctgtgtat ccggcgaaac ccccggcttt tcaggcgaaa
     1321 cggcggacgg cgaatttctc gcgccgctga cgcacgaaaa cgccgttctg cttcgcaagc
     1381 tgtttccgtt taccgcgcca aagccggtgc tgcgggaact gcgctcgttc ggcgtgggcg
     1441 accgcctcgg catcgcgacg cccgggcacc tgcgcgtgtt tgagcagtac gacgcgtttc
     1501 ccgtgctcgc ccagcagtcc atccgcgagc tgactttgac aaagcgcacg tttgcggacg
     1561 tgctggacag cgcaacgttc gcggtcttcc gcgaggattt tcaaaagggg tttggcgcgg
     1621 acggcgacca cctcaaaacg gatgaagaag tggcatacgc gctcgcgaac gggtacacca
     1681 tgatcacgct ggactgcagc gaacatatcc gcaacgatgt gacgggcatg acggacgagc
     1741 aggtgaacgc agcttaccag ccgcgcccgg actgggaaaa gctctactgc ggcaaaacgt
     1801 ttgctgtgga agatcaaaag gtggcgttcg gcgagcagga attccgccgc gcgtgcctga
     1861 tttatgccgc ggcgc
//
LOCUS       DAOMGY010000364         1872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_71606_length_1872_cov_2.964227, whole genome shotgun sequence.
ACCESSION   DAOMGY010000364 DAOMGY010000000
VERSION     DAOMGY010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71606_length_1872_cov_2.964227"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PLR57_06100"
     CDS             complement(<1..963)
                     /locus_tag="PLR57_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQQ41065.1"
                     /translation="MRPEMEKTYDHARVEAALYEKWERNGYFHARPNTGKPPYTIVIP
                     PPNITGRLHMGHAIDETLQDVLIRYKRMSGYEALWMPGTDHASIATEVKIVEQMAKEG
                     ITKKDIGREGFLKRAWAWKEEYGSTIVKQLRYLGSSCDWERERFTMDEGCSKAVTKVF
                     VSLYKKGLIYRGDRIINWCPDCKTALSDAEVEYEEQNSSLWHVRYDAPDKSYSITVAT
                     TRPETMLGDTAVAVHPEDPRYQAIIGKTVVIPAVGREIPIVADEYCEMSFGTGAVKIT
                     PAHDPNDFEVAMRQNLPVLCVFTESGMINEHGGKFEGLDRFACRK"
     misc_binding    complement(1012..1249)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1383..>1872)
                     /locus_tag="PLR57_06105"
     CDS             complement(1383..>1872)
                     /locus_tag="PLR57_06105"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HQQ41066.1"
                     /translation="ESISRMQKVSNFGGYAEGWAQFAEFLVAENQTKYDTNYYMLQFE
                     YNMMFNALLPAILSILVDYYGYTQEALASYLTGIGLDGEYVASIYYPIVVDQPYYFFE
                     YAIGYAQLAQMYRDIKDNLGDNFDQEAFLKTYMDLGPGYFEMIKEKMDVWADNLMIDQ
                     AA"
BASE COUNT          378 a          538 c          495 g          461 t
ORIGIN      
        1 cttacggcag gcgaaacggt cgagcccttc gaacttgccg ccgtgctcgt taatcatgcc
       61 gctctcggta aacacgcaga gcacgggcag gttctgccgc atggcgactt cgaagtcgtt
      121 cgggtcgtgc gcgggcgtaa tcttgaccgc gccggtgcca aagctcattt cgcagtattc
      181 atccgccacg atggggattt cgcgccccac ggcgggaatc acgactgttt ttccgataat
      241 cgcctgatag cgcggatcct ctggatgcac ggcaacagcc gtgtcgccca gcatggtctc
      301 cggacgggtc gtggccacgg tgatggaata gctcttgtcc ggcgcgtcgt aacgcacatg
      361 ccagaggctg ctgttctgct cctcgtattc cacctcggcg tcggagagcg cggttttgca
      421 gtccgggcac cagttaataa tgcggtcgcc gcggtagatc aagcccttct tataaaggct
      481 cacgaacacc ttggtcacgg ccttgctgca gccttcgtcc atggtgaagc gctcacgctc
      541 ccagtcgcag gacgagccga gatagcgaag ctgcttgacg atggtcgagc cgtattcttc
      601 cttccaggcc caggcgcgct ttaagaagcc ttcgcggccg atgtcctttt tcgtgatgcc
      661 ttcttttgcc atctgctcca cgattttcac ttcggttgcg atggaagcgt gatccgtgcc
      721 cggcatccag agcgcttcat agccgctcat gcgcttatag cgaatcaaaa cgtcctgcag
      781 cgtttcgtcg atggcgtgac ccatatgcag cctgcccgtg atgttgggcg gcggaatgac
      841 gatggtatac ggcggtttgc cggtgttggg gcgcgcgtgg aaatagccgt tccgctccca
      901 tttttcatag agcgcggctt ccactctcgc gtggtcgtaa gtcttttcca tttcgggacg
      961 catgttgtct ccccctgcct tctgtcgatc aataaaataa cgcccgcctc gccaaaggac
     1021 gaagcggacg ttcgcggtac cacctttgtt gaacggttcg cgcaaacgcg taagccgttc
     1081 ctctcaattc cttaacgcgg aatacgggaa gcgctgaacg ggcgaacgcc cttttacgtc
     1141 tcctgctccg atgcgacctt ccgcgctgcc cgccagccgt ctctcagcaa attgacggcc
     1201 tctctgatgc gaaactttct cgcgtactcc tcatcttcca agcatttata caatataacc
     1261 agttgccgtc agtctagcgg aaatatcggc ttttgtcaag cgcaagcgcg agcggttaac
     1321 gagcggtaaa aagcaaaacg cccgcagaac tttttccgcg ggcgtcgttc gttttttaat
     1381 gcttatgcgg cctgatcgat catcagattg tcagcccaga cgtccatctt ttccttgatc
     1441 atttcaaagt agccggggcc taggtccata taggttttca ggaacgcttc ctgatcgaag
     1501 ttgtcgccaa gattatcctt gatgtcgcgg tacatctgcg caagctgcgc atagcctatt
     1561 gcatactcaa agaagtaata cggctgatcc acgacgatgg gatagtagat gcttgcaaca
     1621 tactctccgt ccagaccgat gccggtaagg taagaggcaa gcgcctcctg cgtgtatcca
     1681 tagtagtcta cgaggatact gagaattgcg ggcaaaagag cgttgaacat catgttgtat
     1741 tcaaactgca gcatgtaata attcgtgtca tacttcgttt gattctccgc aaccagaaac
     1801 tccgcaaact gcgcccagcc ttcagcgtag ccgccgaagt tggagacttt ttgcatgcgg
     1861 ctgatgcttt ct
//
LOCUS       DAOMGY010000365         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_71663_length_1871_cov_2.874449, whole genome shotgun sequence.
ACCESSION   DAOMGY010000365 DAOMGY010000000
VERSION     DAOMGY010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71663_length_1871_cov_2.874449"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLR57_06110"
     CDS             complement(<1..313)
                     /locus_tag="PLR57_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQQ41067.1"
                     /translation="MTTIDGKRYAKFDLHVHTAETSKCGTVSAPDLVRTYKNNGYDGF
                     VVTDHLHETYISLQNCRDDWQECVTRFLFGYQEAKRVGDEIGFTVALGAELRFPENDS
                     DY"
     gene            complement(355..1368)
                     /locus_tag="PLR57_06115"
     CDS             complement(355..1368)
                     /locus_tag="PLR57_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQQ41068.1"
                     /translation="MKNVTIKDIAKVAGISYATVSRALSGSSEIGEETRERVLRICRE
                     MGYTTNYVARSMVMKQTKLLGLIVANIDNPFMSEMAYHIEMRAREHGYNLMLCNSAHD
                     LNLERNVFELLVSRQVDGIILVPSNAESYVNLKPLISKVPTVFVSENLRDLPESYVSV
                     DNYRGTHIGTEYLYSLGHRNILYFGQRKGSVTHQLRAEGYIDACKELGIEPNLFSSSY
                     PLSSVQAGSQMAKQIFASPFPYTAIFAATDSLALGVFAAAEEAGIRIPEDVSLMGFDN
                     ILYADLPRIRLTTIEQPKKTMATAAVDILLEKITDETSGYSHRILTPTLIVRSSCRDI
                     RQG"
     gene            1626..>1871
                     /locus_tag="PLR57_06120"
     CDS             1626..>1871
                     /locus_tag="PLR57_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41069.1"
                     /translation="MIQAVLFDIGGTLHTVTNSEALARAFARRLIDRLAVYGIEIPTP
                     PEEFAKRLHENAEAYKHWSEVTLRELPAPKIWNEYYLC"
BASE COUNT          385 a          480 c          551 g          455 t
ORIGIN      
        1 ggtaatcgct gtcgttttcc ggaaagcgga gttccgcgcc gagcgcaacg gtaaagccga
       61 tttcgtcgcc gacgcgcttt gcttcctgat agccaaagag aaagcgggtg acgcattcct
      121 gccagtcgtc gcggcagttc tgcagggaaa tatacgtttc gtgcaggtgg tcggtcacca
      181 caaagccgtc gtatccgttg tttttgtagg tgcgtacgag gtccggcgcg ctcacggtgc
      241 cgcatttgct ggtttcggcg gtgtggacat gcaggtcaaa ttttgcgtag cgtttgccat
      301 cgattgtcgt catgaatgtt gttatctcgt ttcagcagta tcggtcatta tcggttaccc
      361 ttgcctgatg tcccggcagg aagagcggac aataagtgtc ggcgtcagga tgcgatggga
      421 atagccggag gtttcgtcgg tgattttttc aagcagaata tccacggcgg cggtcgccat
      481 cgtctttttc ggctgctcga tcgtggtgag ccggatgcgc ggcaggtcgg cgtagaggat
      541 attgtcaaat cccatcagcg aaacgtcttc cggaatgcgg atgcccgcct cctcggcagc
      601 ggcaaacacg ccgagcgcga gggagtccgt tgccgcaaag atagccgtgt aggggaaggg
      661 agacgcaaaa atctgttttg ccatctggct gcccgcctgc acgctgctca gcggatagct
      721 gctgctgaag aggttcggct cgattcccag ttccttgcag gcgtctatat acccttccgc
      781 gcgcaattgg tgcgtaacgc tgcctttgcg ctggccgaag tagagaatgt ttctgtggcc
      841 aagggagtaa aggtattccg ttccaatgtg tgtgccgcgg tagttatcaa cggagacata
      901 gctctccggt aaatcgcgga ggttttcgct gacgaacacc gttggtactt tggagatcag
      961 gggtttgagg tttacatagg attctgcgtt ggagggcacg aggatgatgc cgtctacctg
     1021 gcggctgacg agaagctcaa acacgttgcg ctccagattc agatcgtgcg ccgagttgca
     1081 gagcatgagg ttgtagccgt gttcgcgcgc tcgcatttcg atatgatacg ccatttcgga
     1141 catgaacggg ttgtcgatat tggcaacgat gaggccgagc agcttcgtct gtttcatgac
     1201 catggaacgg gcgacgtagt tggtcgtata tcccatctcg cggcagatgc gaagcacgcg
     1261 ctcgcgcgtt tcctcgccta tttcggagct gcccgaaagc gcccgcgaca cagttgcgta
     1321 cgagatgcct gccaccttgg cgatatcctt tatcgtgaca tttttcatat tctgctgtac
     1381 gctccgatgg gaaaataatg cttcacaatc gcaaacgttt gcgactttca gttgatatta
     1441 caccatcttc aagcatttgt caatgcgcaa attcgcgctt atggggcgcg gctgcctgac
     1501 gcaaaacgtc cgcaagagtc ggaatattgt tgacaaacgc aaaagcacgc attacaatgg
     1561 cattgttcga ttgatatcgc aaacgtttgc gagcattcct gtcatatccg gcggcgaggt
     1621 gacccatgat acaggctgtt ttgttcgaca tcggcggcac gctgcacacc gtcacaaatt
     1681 ccgaggcgct cgctcgtgcg ttcgcgcggc ggcttatcga ccggctcgcg gtgtacggca
     1741 tcgaaattcc cacgccgccc gaggagtttg caaaacgcct ccatgagaac gcggaggcat
     1801 ataagcattg gagcgaggtt acgcttcgcg agctgcccgc gccgaagata tggaacgaat
     1861 actatctttg c
//
LOCUS       DAOMGY010000366         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_72016_length_1865_cov_3.471823, whole genome shotgun sequence.
ACCESSION   DAOMGY010000366 DAOMGY010000000
VERSION     DAOMGY010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72016_length_1865_cov_3.471823"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1865)
                     /locus_tag="PLR57_06125"
     CDS             complement(<1..>1865)
                     /locus_tag="PLR57_06125"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HQQ41070.1"
                     /translation="PAQSLQRVEMARVARAVAEADRAYDDKADVEAVSETFYDLMANL
                     RFLPNSPTLMNAGRPLGQLSACFVLPVEDSMEAIFEAVKNAALIHKSGGGTGFSFSRL
                     RQKGATVRSTGGVASGPVSFMRVFNVATEAVKQGGTRRGANMGILRVDHPDIMEFIRC
                     KSDTSDINNFNISVGLTEKFMRAVEENQDYELIDPKSKAVVKTLSAREVFDEIVDAAW
                     QTGEPGIVFLDRLNRDNVVPSVGEIESTNPCGEQPLLPYESCNLGSIDLVSMLKPDGE
                     FYAFDFDLLEETVHKAVHFLDNVIDVNNYPLEKISSMTRSTRKIGLGVMGFADALLAL
                     GIPYDSAEGIAFAEHLMSFIEETGRAASRELAKTRGAFPLFSESTLKDDEPIRNATVT
                     TIAPTGTLSIIASCSSGVEPVFAFVYVRNVMDGTQMVEVNPMLMQKLHALGLDNEDLL
                     KRIAAEGTIQHCLEIPEDVRRVFVCAHDITPEDHTRMQAAFQRHTDNAVSKTVNFSNS
                     ATRDDVRKVYLLAYHEGCKGVTIYRDGSRSGQVLNIGSVNKDGQPKETPAGLPVAAFG
                     EIEPRPRPTVTRGFTEKMKIGCGTLYITTNYDENGICEVFTSTGKAGGCPSQSEAT"
BASE COUNT          340 a          537 c          574 g          414 t
ORIGIN      
        1 gtggcctcgc tctggctggg gcatccccct gctttgccgg tgctggtaaa cacctcgcag
       61 atgccgtttt catcatagtt ggtggtgata tataaggttc cacagccgat cttcattttt
      121 tccgtaaagc cacgggtcac tgtgggccgc gggcggggtt cgatttcccc aaaggccgcc
      181 accggcagcc ccgcaggcgt ttctttgggc tggccatcct tattcacgga gccaatgttg
      241 agcacctggc cgctgcggct gccgtcacga tagatggtca cgcccttgca cccttcatgg
      301 tacgccagca aatacacttt tcgcacatca tccctggtgg cggaattgct aaagttcacg
      361 gttttgctca ccgcgttgtc cgtatgccgc tggaacgccg cctgcatgcg ggtgtgatct
      421 tccggcgtga tatcgtgcgc gcagacgaac acgcggcgca catcctccgg aatctccaga
      481 cagtgctgaa tcgtgccttc ggcggcgatg cgcttgagca ggtcttcgtt atccagaccc
      541 agcgcgtgga gcttctgcat gagcatcggg ttgacttcga ccatctgggt gccgtccata
      601 acgttgcgga cgtagacgaa cgcaaacacc ggttccacgc cggaggagca ggaagcgata
      661 atcgagagcg tgcccgtcgg cgcaatggtg gtgacggtcg cgttgcggat cggttcgtca
      721 tccttaagcg tgctttcgga aaacagcggg aacgccccgc gcgttttggc gagctcccgg
      781 cttgccgcgc gaccggtttc ttctataaag gacatgagat gttcggcaaa ggcgatgcct
      841 tccgccgaat cgtagggaat tcccagcgcg agaagcgcgt ccgcaaagcc cattacgccg
      901 agcccgatct tgcgggtgct gcgcgtcatg gaggaaatct tttcgagcgg gtagttgttg
      961 acgtcgatta cgttgtcgag gaaatgaacg gccttgtgga cggtttcctc cagcaggtca
     1021 aagtcgaacg cgtagaattc tccgtcgggt ttgagcatgg aaacgaggtc gatggaaccc
     1081 agattgcagc tttcgtaggg aagaagcggc tgctctccgc aggggttcgt gctctcgatt
     1141 tcgccgacgg aaggcacgac gttgtcgcgg ttgagcctgt ccagaaatac gattcccggt
     1201 tcgcccgtct gccaggcggc gtccacgatt tcgtcaaaaa cctcccgtgc cgagagcgtt
     1261 ttaactacgg ccttgctctt ggggtcgatc agctcgtagt cctgattttc ctcgaccgcg
     1321 cgcatgaatt tttccgtcag gccgacggag atgttgaagt tgttgatatc gctcgtatcg
     1381 ctcttgcagc ggatgaattc catgatgtcc gggtgatcga cacggaggat gcccatgttc
     1441 gcgccgcgcc gcgtgccgcc ctgcttgacg gcttcggttg caacgttgaa cacgcgcata
     1501 aaactgacgg ggccgctggc aacgccgccc gtggagcgga cggtcgcgcc cttctggcgc
     1561 aggcgcgaaa acgaaaagcc ggtgccgccg ccggatttat ggatgagagc agcgtttttc
     1621 accgcttcga agatggcttc catgctgtcc tcaacgggca gcacaaagca ggcggagagc
     1681 tggccgagcg gacggcccgc gttcatcagc gtcggggagt tgggtagaaa tcggaggttc
     1741 gccatgagat cataaaatgt ttcgctcacg gcttccacgt ccgctttgtc gtcataggcg
     1801 cggtccgctt ccgcaaccgc gcgcgcgacg cgcgccatct cgacgcgttg aaggctttgg
     1861 gctgg
//
LOCUS       DAOMGY010000367         1863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_72157_length_1863_cov_3.087389, whole genome shotgun sequence.
ACCESSION   DAOMGY010000367 DAOMGY010000000
VERSION     DAOMGY010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72157_length_1863_cov_3.087389"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PLR57_06130"
     CDS             <1..627
                     /locus_tag="PLR57_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HQQ41071.1"
                     /translation="PPHFQPEWPEQFELFSFLEFACGIPQILFVVTTYKQNDLPNVCP
                     HTWSSFVSGKDGCGYYAVLGGLMERTHTYRNILRDRCFCINFLSSTYYDDMMRAISQN
                     EDTRDEFAVCGFTKAECKTIHAPRICESFLSLECELSDVAEAAGGALKLIIGKTTFVT
                     LKEGYGEGIDLKYGENGFSYNVHSPINTTTGICDPVAIAELEIARKLV"
     gene            complement(685..1104)
                     /locus_tag="PLR57_06135"
     CDS             complement(685..1104)
                     /locus_tag="PLR57_06135"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HQQ41072.1"
                     /translation="MKAPAKNFIRELQSKDIKFDSKEIDGGKDWVMVRFTGKNCPSIT
                     TQFFFDADGESVSAKCFTICQVPKDKRNAMLDAVNTLNVKYRWLSFYIDGDEEVTCNA
                     DSVLNAETSAEVCFNLLVRFITIVDEAYPELMKALWK"
     gene            complement(1274..>1863)
                     /locus_tag="PLR57_06140"
     CDS             complement(1274..>1863)
                     /locus_tag="PLR57_06140"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQQ41073.1"
                     /translation="QSVVCFKAENGEWTEERVMICSTGRSKLLTPRGTFALMRQYLYK
                     KMGQVDGQNVYSQYASRITGSYLFHSVPIGGENRYSQENGKHQMIVEYYERLGTPASG
                     GCVRLRCVDAYWVYMNCPVGTTVIITDDSGPQAPEIPALIYEEPYMDKRHELGWDPSD
                     PDPENPYHAIYQPEFVITTPVEDKSVDDKIEDAGN"
BASE COUNT          458 a          476 c          453 g          476 t
ORIGIN      
        1 cccccccact ttcagccgga atggccagag cagttcgagc tgttttcgtt tttggaattt
       61 gcctgcggga ttccacagat tctgtttgtc gtgaccactt acaaacaaaa tgatttgccg
      121 aatgtctgcc cgcatacctg gagctcgttt gtcagcggaa aagacggctg cggctattat
      181 gcagttctcg gcgggctgat ggagcgcacg catacttacc ggaatattct tcgtgatcga
      241 tgcttttgca tcaacttcct ctcaagcacc tactacgacg acatgatgcg ggcgatttca
      301 caaaacgaag atacgcggga cgaattcgcc gtttgcggct ttacgaaagc ggagtgcaaa
      361 acaattcatg cgccgcgcat ctgcgaaagc tttttatcgc ttgagtgtga actcagcgac
      421 gttgcggaag ccgcaggcgg cgcgctgaag ctaatcatcg gcaaaacgac gtttgtaacg
      481 ctaaaagaag gttatggtga aggaattgac ttgaagtacg gcgaaaacgg tttttcatac
      541 aacgttcatt cgcctatcaa tacgactacc gggatttgcg atcccgttgc cattgccgaa
      601 ctggaaatcg cgcggaaact agtgtagctt aaaacaaata agagcccgcc gatgtaaaat
      661 cggcgggctc ttcggttgtt aaagttattt ccagagcgct ttcataagct ccggatatgc
      721 ttcatcaacg atcgtaatga agcggacgag aaggttgaag cagacttccg cagaggtttc
      781 tgcattgagc acagagtccg cattgcaggt gacttcttcg tcgccgtcaa tgtagaagga
      841 aagccagcgg tacttgacgt tcagcgtgtt gaccgcgtcg agcatggcgt ttctcttgtc
      901 cttgggaacc tggcaaatcg taaagcactt tgcgcttacg ctttcgccgt ccgcatcaaa
      961 gaagaactgc gtcgtgattg aggggcaatt cttaccagtg aaacgcacca tcacccaatc
     1021 tttaccgcca tcgatctcct tggaatcgaa cttgatgtcc ttcgactgca attcgcgaat
     1081 aaaattcttc gccggtgcct tcatatgata actcctccga caattttctt atagaatatc
     1141 acgaaaataa ccccatgtca aagtttggag cgcaaaaaaa cagcagaaat ttgtcggatc
     1201 attgcataaa tatgtatgca tattgcacac atcatgcaat cgttttgcag cgaagctttt
     1261 cagcggttga ccgtcagttt ccggcatcct caattttatc gtcaacggat ttgtcctcaa
     1321 ctggagtcgt gattacgaat tccggctggt aaatcgcatg atatggattc tccgggtcgg
     1381 gatcgctggg atcccagcca agttcatgcc ttttatccat atacggttct tcgtagatca
     1441 gcgcgggaat ctccggcgcc tgcggaccgc tgtcatccgt aatgatgacg gtcgtgccga
     1501 cgggacaatt catataaacc caatacgcgt ctacacaacg gaggcgcacg catccgccgg
     1561 acgcgggcgt accaagacgt tcgtagtatt caacgatcat ctgatgcttg ccgttttcct
     1621 gagagtaacg attttccccg ccaatcggca cggagtggaa caggtaactg cctgtaatac
     1681 ggctggcata ttgggaatag acgttctggc cgtctacctg acccatcttc ttatataaat
     1741 actgccgcat cagcgcaaac gtgccgcgcg gggtcaacag tttactgcgt cccgtggagc
     1801 agatcattac gcgttcttcc gtccattccc cgttttccgc cttaaagcaa accacggact
     1861 gtg
//
LOCUS       DAOMGY010000368         1861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_72252_length_1861_cov_3.658361, whole genome shotgun sequence.
ACCESSION   DAOMGY010000368 DAOMGY010000000
VERSION     DAOMGY010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72252_length_1861_cov_3.658361"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /gene="ricT"
                     /locus_tag="PLR57_06145"
     CDS             complement(<1..1089)
                     /gene="ricT"
                     /locus_tag="PLR57_06145"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HQQ41074.1"
                     /translation="MKVIGVRFKSSGRIYYFDPLEYTFNEGDGVIVETARGQEYGEVA
                     QVAMEVEESVIVSPLKPVIRPATEQDSVMREQNAAKEGDAFHVCQQKIQQHKLDMHLV
                     SAEYSFNGSKLVFYFTADERVDFRELVKDLASQFRTRIELRQIGVRDEAKMLGGLGSC
                     GRVVCCKAFLQDFHPVSIKMAKEQNLSLSPTKISGLCGRLMCCLQYEQSAYEEMKRRM
                     PHINREVLTPDGVGTVVENNAITEKTRVRLQLPDGSIDVREFNYMLLGRPGEPLPVPP
                     SEPKPEPVKEAAEEPKPAPAQEATPERSPDKKRRRRGGRGKNGNRPNAPQGEGGQPAQ
                     QQQPRPQQQSQQPQQPRPPQQPQRAPQQR"
     gene            complement(1098..>1861)
                     /locus_tag="PLR57_06150"
     CDS             complement(1098..>1861)
                     /locus_tag="PLR57_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HQQ41075.1"
                     /translation="LLSSAAMQGFNGGNRAFVFINAQNMSVQIQNTLLKTLEEPHEDT
                     LLLLTGNEFGLLPTIRSRCVILRLGAESLEATASELRADGMSAADARFFAALADGLAG
                     RARAFSEPEVAEFRTRALEILERAAFSYSPYADAAELVTSFSADADPESEAEDEPKAK
                     AKKKKRGDAALANRLFEIWQSVFRDALTHQTGGVKPINADQAELIARIASRFTSARIQ
                     GIIELLARAQTRLSGGASVYLTVDSALTGLFIKES"
BASE COUNT          321 a          590 c          525 g          425 t
ORIGIN      
        1 acgctgctga ggcgctctct gcggctgctg cggaggtctc ggctgctgtg gctgctgaga
       61 ttgttgctgc ggccttggtt gctgttgttg cgcaggctga ccgccttcac cctgcggggc
      121 attcgggcgg ttgccgttct tgccgcgacc acccctgcgg cggcgtttct tatcagggga
      181 gcgttccggc gtcgcttcct gcgcgggagc gggcttcggc tcttcagccg cttctttgac
      241 gggttccggt ttcggctcgc tcggcggcac aggcagcggt tcgccgggcc ttccgaggag
      301 catgtagttg aactcgcgca cgtcgatcga cccgtccggc agttgcaggc gcactctggt
      361 cttttcggta atagcgttat tttcaacgac ggttccaacg ccgtctggcg tcagcacttc
      421 gcggttgata tgcggcatgc gccgcttcat ctcttcatat gcggactgct cgtactgcag
      481 gcagcacatc agccttccgc aaagccccga aatcttggtc gggctcagcg aaagattctg
      541 ttcctttgcc attttaatcg aaacgggatg aaaatcctgt aagaacgcct tgcagcagac
      601 gacccttccg caggaaccga gcccacccag catcttggct tcgtcgcgaa cgccgatctg
      661 cctgagctca atgcgcgtgc ggaactggga cgcgaggtcc ttgaccagtt cgcggaaatc
      721 gacccgttcg tccgcagtga agtaaaacac gagcttgctg ccgttgaatg agtattccgc
      781 gctcacaagg tgcatatcca gcttgtgctg ctgaatcttc tgctggcaga cgtggaaggc
      841 gtcgccttcc ttagccgcgt tctgttcgcg catcacggaa tcctgttcgg ttgcggggcg
      901 aatcacgggc ttgagcgggg aaacgatgac cgattcttcc acttccatcg caacctgtgc
      961 cacttcgccg tattcctgcc cgcgcgccgt ttcgacaata accccgtcgc cttcgttaaa
     1021 cgtatattcc aacggatcga aataatagat gcgtccgctc gatttgaacc gtacgccgat
     1081 aactttcatt ttaataccta gctctccttt ataaacagcc ccgtcagcgc gctgtccacg
     1141 gtcaagtaga cgcttgcgcc gcccgaaagg cgcgtctgcg ccctggctaa cagttctatg
     1201 ataccttgaa tgcgtgcgct tgtaaagcgc gaggcaattc gcgcaatcag ttcggcctga
     1261 tcggcgttga tgggctttac cccaccggtc tggtgcgtaa gcgcgtcgcg aaacacgctc
     1321 tgccagattt cgaacagcct gtttgccagc gccgcgtccc cgcgcttctt ctttttcgcc
     1381 ttcgctttgg gttcatcctc cgcttccgac tccggatctg cgtccgcgga aaagcttgtc
     1441 accagctccg ccgcgtccgc ataaggcgaa taggaaaacg ccgcgcgttc cagtatctcc
     1501 agcgcgcgtg tgcgaaattc cgccacttcc ggctccgaaa aagcgcgcgc ccgccccgca
     1561 agcccgtccg ccagcgcggc gaaaaacctt gcgtccgccg cgctcatgcc gtctgcccgc
     1621 agttcggaag ccgtcgcttc caggctctcc gcgccgaggc gcagaatcac gcagcgggag
     1681 cgaatcgtcg gcagcagccc gaactcgttt ccggtcagca gaagaagcgt gtcctcgtgc
     1741 ggctcttcca gcgttttgag cagcgtgttt tgaatctgca cgctcatgtt ctgcgcgttg
     1801 atgaacacaa acgcgcggtt gccgccgttg aatccctgca tcgccgcgga agacaaaagg
     1861 g
//
LOCUS       DAOMGY010000369         1853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_72752_length_1853_cov_3.661846, whole genome shotgun sequence.
ACCESSION   DAOMGY010000369 DAOMGY010000000
VERSION     DAOMGY010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72752_length_1853_cov_3.661846"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLR57_06155"
     CDS             complement(<1..848)
                     /locus_tag="PLR57_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41076.1"
                     /translation="MTILNVRSLIALFCALALGLSALSDCAAQTSTESTATLTDTAQQ
                     TTDSSATYVQVQSIENGTITALIGTLSQNAPGGQQGTPPSGDNANGTQGTPPEMPSGD
                     SANNAQGTAPSGDSANGAQGSAPQQPSGDNANGQQGTPPSGGATGGMGGFTAGTETLT
                     FTVSSATAITLADGTTGTIDSIAAGDVLAITLSSANVAETITVQSLGMPQDMNAGGQP
                     GANFGGSSEVTNGTSANTIGTDSTVTDETYTSSGDDENALRIDGAVVTLDGITVSKTG
                     GASSNT"
     gene            1143..>1853
                     /locus_tag="PLR57_06160"
     CDS             1143..>1853
                     /locus_tag="PLR57_06160"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQQ41077.1"
                     /translation="MKRLFALLAALMLAGGAPLNNAKPDIAVVQAGVDLMMIVTHPGD
                     EYLYFGGVLPQYVSEQGYTAVVVYMTSVDETQRQQATAALKALGVSEAPVFAGFANVY
                     ADNTDTIKDWWGEKETINFLVSVIRQYQPAVVVSHDLKGEYGDGANMLTAKCVQAAVK
                     YAAESSVDKTSLALYGPWQTFRLFLHLYTKGKASVSLDRTAPVAAFSGRSSVDVETDL
                     YESFTESARYALDVGDEVY"
BASE COUNT          359 a          523 c          557 g          414 t
ORIGIN      
        1 tccgtattgg aggaagcgcc tccagttttg ctgaccgtga tgccgtcgag ggtgacgaca
       61 gcgccgtcga tacgaagcgc gttctcgtcg tcgccggagg acgtataggt ttcatctgtt
      121 acagtagaat ccgtgccgat ggtattcgcg ctcgtgccgt ttgtgacctc cgaactgccg
      181 ccaaagttcg ctcccggctg tccgcccgcg ttcatatcct gcggcatccc taatgactga
      241 acggtaatcg tttcggccac gttcgcgctc gagagcgtga ttgcgagcac gtctcccgcg
      301 gcgatgctgt cgatggttcc cgttgtgccg tccgcaagcg tgatggcggt tgcgctgcta
      361 acggtaaacg tcagcgtttc ggtgcctgcc gtaaagccgc ccatgccgcc ggtcgctccg
      421 ccggaaggag gcgtgccctg ctgcccgttc gcgttgtcgc ccgaaggctg ctgaggagcc
      481 gagccctgcg ctccattcgc gctgtcgccc gaaggcgcgg ttccctgtgc attgtttgcg
      541 ctgtcgccgg aaggcatctc cggcggggtt ccctgcgtgc cgttagcgtt gtcgccggaa
      601 ggcggcgtgc cttgctggcc gccgggcgcg ttctgcgaaa gcgtgccgat cagcgcggta
      661 atggttccgt tttcgatgct ctgcacctga acgtatgtcg cgctgctgtc ggtggtttgc
      721 tgtgccgtgt cggtaagagt tgccgtggac tccgtggatg tttgcgcggc acagtcggag
      781 agcgcgctga ggccgagcgc cagcgcacag aaaagcgcaa tcagagagcg aacattcaaa
      841 attgtcatcc cctcattgtt taatactaga attttatgcc cgctttgtgg cgacaatgta
      901 gtctatccat ggtatttttt cttcgaattt tgaaatttca aaacttccaa tttcgcagcg
      961 cagaaagcat gccgcaaagt tgacgcggcg gatggaaaaa tactacaata gagtatctgg
     1021 tggagtttat agtttgcata accgcccgcc ggacgccgcg gctgattccg gctgtttgta
     1081 aacccgtccg ggaacccgcg cgcggtaaaa atcttttgaa acaatgccaa aggtgtgaaa
     1141 caatgaagcg actctttgct ctcctggccg cgctgatgct tgcaggcggc gcgccgctga
     1201 acaatgcaaa accggatatc gccgtcgtac aggccggcgt cgatctgatg atgatcgtga
     1261 cccatcccgg agacgaatac ctctacttcg gcggcgttct gccgcagtat gtcagcgagc
     1321 aggggtatac ggcggtcgtt gtttacatga caagtgtgga cgaaacgcag cgtcagcagg
     1381 caaccgccgc gctaaaagcg ctgggcgtca gcgaagcgcc ggtgttcgcc gggtttgcga
     1441 acgtctacgc cgataatact gataccatca aggactggtg gggcgaaaaa gaaactatca
     1501 actttttagt gagcgtcatc cgccagtacc agccggcggt cgtcgtaagc cacgatctga
     1561 aaggcgaata cggcgacggc gcgaatatgc tcacggccaa gtgcgttcag gccgcggtga
     1621 aatacgccgc cgaaagcagc gtggataaaa catcccttgc tctttacggg ccctggcaga
     1681 ccttccgtct gtttctgcat ttatatacga aaggcaaagc gagcgtttcg ctcgaccgca
     1741 cggcgccggt tgccgcgttc tcaggccgct cgtcggtcga tgtggagaca gacctttacg
     1801 aatcgtttac cgaatccgcc cgctacgcgc tggacgtcgg ggacgaagta tac
//
LOCUS       DAOMGY010000370         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_73124_length_1847_cov_2.521763, whole genome shotgun sequence.
ACCESSION   DAOMGY010000370 DAOMGY010000000
VERSION     DAOMGY010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73124_length_1847_cov_2.521763"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PLR57_06165"
     CDS             <1..960
                     /locus_tag="PLR57_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41078.1"
                     /translation="TATSYAPFHYGGSFGLTLAAMGFVSASIGGVIYLGRLKKKGLID
                     QTIINADEMENLSAETITEKGEIPLSESLDKMTVQIGLVVFMYMAAYLLMYVVSLGLD
                     QLGGFFSSTVKPLIWGFNFLIGTVMAILVRNVLNGLTRRGRRQYINNFMLARISGVMF
                     DIMVVASIAAINLSAFSHREFIIPLIAICVVGAVATYLQLGFISRRLYPDYPHEAFLS
                     LYGMLTGTASTGVILLREIDPLFKTPAATNLVYQQLWAIVFGFPMLLLLGYAPIGLTA
                     DPATSNMTNLWITLAALTGLFIVMNLILFRKQLFGRKKSKQAS"
     gene            complement(1040..>1847)
                     /locus_tag="PLR57_06170"
     CDS             complement(1040..>1847)
                     /locus_tag="PLR57_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HQQ41079.1"
                     /translation="SGSFGGINLEDISAPRCFEIERKLKEKCDIPIFHDDQHGTAVVT
                     LAGLINALKVVGKKKSDVKIVINGAGAAAISVTKLLLSDGFKSVILCDRSGIIYAGRK
                     DNMNWIKEEMAQVTNAEKKTGSLADALVDADVFIGVSAPNMVTVDMVKTMAKDAIVFA
                     CANPTPEIFPDDAKAGGAAVIATGRSDFPNQINNVLAFPGIFRGTFDVRARDINDEMK
                     IAAAYALAGLISDEERSADYVIPKAFDPRVGKTVAAAVAEAARKSGVARL"
BASE COUNT          342 a          542 c          518 g          445 t
ORIGIN      
        1 acggcaacga gctatgcgcc gttccattac ggcggcagtt tcggcctcac gctcgccgca
       61 atgggctttg tttcggcaag catcggcggt gtgatctacc ttggccgtct caagaaaaag
      121 ggattgatcg atcagacaat tatcaacgcc gatgaaatgg aaaacctctc cgccgaaacc
      181 atcacggaga aaggcgaaat cccgctctcc gaatcgctgg ataagatgac cgtccagatc
      241 gggcttgtcg tgtttatgta tatggcggca tacttattga tgtatgtagt ctcgctcggg
      301 ctggatcagc tcggcggatt tttctcctcc accgtaaaac cgctgatctg gggctttaac
      361 ttcctgatcg gcaccgtgat ggcaattctt gttcggaacg tcctcaacgg gctgacccgg
      421 cgcggccgtc gccagtatat caacaacttc atgcttgcac gcatctccgg cgtgatgttc
      481 gatattatgg tggttgccag cattgccgcc atcaaccttt ccgcgttctc ccatcgggag
      541 ttcatcattc ctctaattgc tatctgcgtc gtaggcgcgg ttgccaccta tctccagctt
      601 ggatttattt cgcggcggct ctacccggac tacccgcacg aagcgttcct ttcgctctac
      661 ggcatgctga ccggcaccgc cagcacgggc gtcatccttc tgcgcgaaat cgacccgctg
      721 ttcaagacgc ccgccgcgac gaaccttgtt taccagcagc tctgggcgat cgtgttcggc
      781 ttcccgatgc tgctgctgct tggctacgcg cccataggac taacggcgga tccggcgacg
      841 agcaatatga ccaacctatg gatcacactt gccgctttga cgggcttatt catcgtcatg
      901 aacctgattc tgttccgcaa gcagctgttc gggcgtaaaa agagcaagca ggcttcctaa
      961 agccgtttct ttcgtgcaac gcacgataaa taatcacaaa aaaatgaccg cttccgcggt
     1021 catttttttg tgttgcttct tacagacggg cgactccgct tttgcgtgcg gcttcggcga
     1081 ccgccgccgc gacggtcttg ccgacgcggg ggtcaaacgc cttggggatg acgtaatccg
     1141 cgctgcgttc ttcatccgaa atgagcccgg ccagcgcgta cgccgcggcg atcttcattt
     1201 cgtcattgat gtcgcgcgcg cgcacgtcga acgtgccgcg gaagatgccc gggaaggcca
     1261 gaacgttgtt gatctggttc gggaagtcgc tgcggccggt tgcgatgacc gcagccccgc
     1321 ccgctttggc gtcgtccggg aagatttccg gtgtggggtt tgcgcaggca aagacgatgg
     1381 cgtccttggc catggtctta accatatcga cggtgaccat gttcggggcg gatacgccga
     1441 tgaacacgtc cgcgtcgacc agcgcgtccg caagcgaacc cgttttcttt tcggcgttcg
     1501 taacctgcgc catctcttct ttgatccagt tcatgttgtc cttgcgcccg gcgtagataa
     1561 tgccgctgcg gtcgcagagg atcacgctct taaagccgtc cgaaagcagg agcttggtca
     1621 cgctgatggc ggcagctcct gcgccattga tgacgatttt gacgtccgac ttctttttgc
     1681 ctacgacctt gagggcgttg atgaggcccg cgagggtaac aaccgccgtg ccgtgctggt
     1741 cgtcatggaa aatcgggatg tcgcactttt ccttcagctt gcgttcgatt tcgaagcagc
     1801 ggggcgcgga gatgtcctcc aggttgatgc cgccgaagct gccgctg
//
LOCUS       DAOMGY010000371         1839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_73619_length_1839_cov_3.608744, whole genome shotgun sequence.
ACCESSION   DAOMGY010000371 DAOMGY010000000
VERSION     DAOMGY010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73619_length_1839_cov_3.608744"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PLR57_06175"
     CDS             <1..802
                     /locus_tag="PLR57_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQQ41080.1"
                     /translation="NLARQGVRTALLTAVGDDVYGEKILRDSAAAGIDVSHVLLKPGV
                     GSSCYIAMLDERGDMMLAMSDMRIISDLTGDYLNANRALLQNAAAIVCDACLPCAFLD
                     ELVSGVASGVRVLIDPVSTAYARSMEPIAGKFFGIKPNEMELAILSGKPTETETQVER
                     ACEALLNRGVTCVAVSRGSRGCYYADASGARMFRALRPVERMANATGAGDAFLAGFVH
                     GLIDGVPLERQLDYALASGITAIESLSTINPEMSDARVKENIELYRVL"
     gene            920..1834
                     /locus_tag="PLR57_06180"
     CDS             920..1834
                     /locus_tag="PLR57_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HQQ41081.1"
                     /translation="MNRYLDISPEVQAAIAENKPVVVLESTILSHGMPYPENVGFAAE
                     VEKVVRGEGAIPATAALIHGRIKVGLTPAELDIMCKAENVGKVSRRDMATYLATGQNG
                     ATTVATTMICAAMAGLKVFATGGIGGVHRHGETTMDVSADLQELKQTSVAVVCAGAKQ
                     ILDIGRTLEYLETMGVPVIGNGTKDFPAFYCRKSGFGVDYAAKDEREIAKIIKTKWDL
                     GLTGGVLIGNPIPEEYALDFDYMEGIINRALAEAEEKGVRGKDITPFLLAKVKELTKG
                     VSFASNVQLAYNNARAAAKISKALCELN"
BASE COUNT          421 a          501 c          582 g          335 t
ORIGIN      
        1 aaaccttgcg cggcagggcg tgcgcaccgc gctgctcacc gccgtgggcg acgacgtgta
       61 cggcgaaaaa atcctgcggg acagcgcggc ggcgggcatc gacgtttctc acgtgctgct
      121 caagccgggc gttggctcct cctgctatat cgccatgctc gacgagcgcg gggatatgat
      181 gctcgccatg tcggacatgc gcatcatttc ggatttaacg ggcgactacc tgaacgcaaa
      241 ccgcgcgctg ctgcaaaatg ccgccgcgat cgtctgcgac gcctgcctgc cctgcgcgtt
      301 tctcgacgag ctggtttccg gcgtggcaag tggcgttcgc gtgctgatcg accccgtttc
      361 caccgcgtac gcgcgcagca tggagccgat tgcgggcaag tttttcggta tcaagcccaa
      421 cgaaatggaa ctcgccatcc tctccggcaa acccacggag accgaaacgc aggtcgagcg
      481 ggcctgtgaa gcgctgctca acagaggcgt aacctgcgtt gcagtcagcc gcggctcgcg
      541 gggatgctat tatgccgacg caagcggggc aaggatgttc cgcgcactgc gccccgtgga
      601 gcgcatggca aacgccacgg gcgcgggaga cgcgtttctg gcggggtttg tgcacggcct
      661 gatcgatggc gtgccgctgg agcggcagct cgattacgcg ctggcaagcg gcatcacggc
      721 aatcgagagc ctgagcacga tcaatcccga aatgagcgac gcgcgcgtca aggaaaatat
      781 tgagctttac agggtactgt aatactgtaa gcggaaatga atgataagaa atattgagct
      841 ttacagagcg ctgtaataca ttttacgaat caaacgaacg gctgcgcggc agtcaaagga
      901 gaataacatg aagaatgaaa tgaaccgata tttggatatt tcgccggaag tgcaggcggc
      961 aattgcggag aacaagccgg ttgtcgtgct ggaaagcacg atattaagcc acggtatgcc
     1021 ctacccggaa aacgtaggct ttgctgccga ggtggaaaag gtcgtccgcg gggagggcgc
     1081 aattcccgca acggcagcgc tgattcacgg gcgcatcaag gttgggctta ctcctgcgga
     1141 gctcgacatc atgtgcaagg ccgaaaacgt aggcaaagtc agccgaaggg atatggcaac
     1201 ataccttgcg accggacaaa acggcgcaac gaccgttgcc accaccatga tctgcgccgc
     1261 gatggcgggg cttaaggtgt tcgccaccgg cggcatcggt ggcgtgcatc gccacggaga
     1321 aacgaccatg gacgtttccg ccgacttgca ggagctcaag cagacgtccg ttgcggtggt
     1381 ctgcgcgggc gcaaagcaga tactcgatat cggcagaacg ctggaatatc tcgaaaccat
     1441 gggcgttccg gtcatcggca acggtacaaa ggattttccc gcgttctact gcagaaagag
     1501 cggcttcggc gtggactacg cggcaaagga cgagcgggag attgcaaaga tcattaagac
     1561 caaatgggat ttgggtctca cgggcggcgt gctgattggc aacccgattc cggaagagta
     1621 cgcgctggac tttgactata tggaaggcat tatcaaccgg gcgctggccg aagcggaaga
     1681 aaagggcgtg cgcggcaagg acattacgcc gtttttgctg gcaaaggtca aggagctgac
     1741 caagggtgtt tcgtttgctt ccaacgttca gctggcgtac aacaacgcgc gcgcggcggc
     1801 aaagatctcc aaagcgctct gcgaactcaa ctgacctcg
//
LOCUS       DAOMGY010000372         1838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_73700_length_1838_cov_3.039821, whole genome shotgun sequence.
ACCESSION   DAOMGY010000372 DAOMGY010000000
VERSION     DAOMGY010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73700_length_1838_cov_3.039821"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /gene="aspS"
                     /locus_tag="PLR57_06185"
     CDS             complement(<1..990)
                     /gene="aspS"
                     /locus_tag="PLR57_06185"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQQ41082.1"
                     /translation="MSEFLGGWKRTNYCANFTKADAGKEVTLMGWANVRRDLGALVFV
                     QLRDRTGLMQVVFNSTILSKEDFDRACTIRSEFVLAVKGKLVLRTGDMINANMATGEL
                     EVIVSEFKILSKSETPPFDISDDCRANELLRLKYRYLDLRRPSMQKNLLMRHKIAFIA
                     REYFDEQNFVEIETPILTKSTPEGARDYLVPSRVHPGKFYALPQSPQLFKQLLMLAGY
                     DRYFQIARCFRDEDLRADRQPDFTQIDIEMSFVEAEDVQSVNEGFLKRLFKETLDVDI
                     ETPLPRLKYADAMARYGSDKPDTRFGLELVDLSDVVKSSEFSVFQSALASGGSV"
     gene            complement(1041..>1838)
                     /gene="hisS"
                     /locus_tag="PLR57_06190"
     CDS             complement(1041..>1838)
                     /gene="hisS"
                     /locus_tag="PLR57_06190"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HQQ41083.1"
                     /translation="LGCEGLTVRLNSIGCPKCRPAYHEALKTYLRGHVDELCETCKVR
                     LEQNPLRVLDCKVESCQKVANAAPVISDYLCDECRDHQSKLIVLLDGMGISYELDPRL
                     VRGLDYYTKTVVEIVSNSIGSQSAVCGGGRYDGLVEEIGGPHVPAVGFGLGMERLLLV
                     LESIGKQIPKPMRYDLYLASMGEKAEEAAFSLAFQLRKAGVSVEIDHVGRSVKAQLKY
                     ADKIGARYVLVLGENELAANKGKLKRMSDGQETELALTAEEIQKALD"
BASE COUNT          363 a          536 c          508 g          431 t
ORIGIN      
        1 gacgctgccg ccgctggcaa gcgcgctctg gaaaacggag aactcgctcg acttgaccac
       61 gtccgaaaga tcgaccagtt ccagcccgaa gcgcgtatcc ggtttatcgg aaccgtagcg
      121 cgccatcgcg tcggcgtact tgaggcgcgg caaaggcgtt tcgatatcca catccagcgt
      181 ttccttaaag agtcgcttga ggaagccttc gttgacggac tgcacatcct ccgcttccac
      241 aaaggacatc tcgatatcga tctgcgtaaa gtccggttga cggtccgctc ggaggtcttc
      301 gtctctaaag cagcgggcaa tctggaaata ccggtcgtaa ccagccagca ttaagagctg
      361 tttgaagagc tgcgggctct gcggcagcgc gtagaacttg ccgggatgca cgcggctggg
      421 cacgagatag tcccgcgcgc cttccggcgt gctcttggta agaatcgggg tttcgatttc
      481 cacaaagttc tgctcgtcaa agtattcccg cgcgatgaac gcgatcttat gccgcatgag
      541 caggttcttc tgcatgctgg gacggcgaag gtcgagatag cggtacttaa gtcgcagcag
      601 ttcgtttgca cggcaatcgt cgctgatgtc gaacggcggc gtttcgctct tgctcagaat
      661 cttgaattcg ctgacgatga cctccagttc gccggtcgcc atattggcgt tgatcatgtc
      721 gccggtgcgg agaacgagtt tgcccttgac cgcaagcacg aattccgaac ggattgtgca
      781 ggcgcggtcg aaatcttcct tgctcaaaat cgtggaattg aacaccacct gcatcagccc
      841 cgtgcggtcg cgcagctgca caaacaccag tgcgccgagg tcgcgccgaa cgtttgccca
      901 gcccatcagg gtaacttcct tccctgcgtc cgccttggta aagtttgcgc agtaattagt
      961 tcgcttccag ccgcctaaaa attcactcat tggttcgctc ctcatatgat gaatattttg
     1021 aatgcctgtt tattgaatgt ttagtccagc gctttttgaa tctcctcagc cgtgagtgca
     1081 agctccgttt cctgaccgtc gctcatgcgc ttgagtttgc ctttgttcgc ggccaactcg
     1141 ttttcgccga gtacgagaac atatctcgcg ccaatcttat cggcatactt gagctgcgct
     1201 tttacgcttc tgccgacatg gtcgatctcg acggaaacac ccgctttgcg gagctggaac
     1261 gccagcgaaa aagcagcttc ttcggctttt tcgcccatgc tcgcgagata gagatcgtag
     1321 cgcatgggtt tgggaatctg ctttccaatg ctttccagca ctagcagcag ccgctccatg
     1381 cctaggccga agcccaccgc cggaacgtgc ggcccgccga tttcctccac caagccgtcg
     1441 tatctgccgc cgccgcaaac cgcgctctgg gagccgatgc tgttggaaac gatctcgacg
     1501 acggtcttgg tgtagtaatc cagcccgcgc acgagccgcg ggtcgagttc gtaacttatt
     1561 cccatgccgt cgagcaggac gatcagcttg ctctggtggt cgcggcattc gtcgcaaagg
     1621 tagtccgaaa tgaccggggc ggcattagcc actttctggc aggattccac cttgcagtcc
     1681 agcacgcgaa gggggttttg ttccagacgc accttacagg tttcgcaaag ctcgtccaca
     1741 tgcccgcgca gatacgtttt gagcgcttcg tggtaagcgg gtctgcattt gggacagcct
     1801 atgctgttga ggcgaacggt cagcccttcg cagccgag
//
LOCUS       DAOMGY010000373         1837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_73764_length_1837_cov_2.705387, whole genome shotgun sequence.
ACCESSION   DAOMGY010000373 DAOMGY010000000
VERSION     DAOMGY010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73764_length_1837_cov_2.705387"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLR57_06195"
     CDS             complement(<1..226)
                     /locus_tag="PLR57_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41084.1"
                     /translation="MDRIEELTDEASEIGIDVQQREIPVPGMSAAYIKTETGEHIILR
                     RDGTQAERACWLAEELGHHYTGADRRLQPQG"
     gene            complement(269..601)
                     /locus_tag="PLR57_06200"
     CDS             complement(269..601)
                     /locus_tag="PLR57_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQQ41085.1"
                     /translation="MTLTEKLDLLLGERGLNKSQLAAQSGVPYTTIASLYEKGAENVK
                     RSTLLALARFLNVTLDFLADDSVTERAPRILASAGHFDVAQLTPEGRERYEEYIEYLA
                     DKFSKENG"
     gene            801..1724
                     /locus_tag="PLR57_06205"
     CDS             801..1724
                     /locus_tag="PLR57_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HQQ41086.1"
                     /translation="MNALTELNFDKKLMLIVNPVAGRKQSLRLVPEIVRIFMDAGYLV
                     TVMVTKAQGDATAFAEHFSCSYDAVVCVGGDGTLSETAAGIARCEKAVPLGYIPAGST
                     NVFATAHGLSNDILTAARNIASGGVKWIDTGLFNARSFAFIAAFGAFSWTSYATPQNL
                     KNILGTPAYFFDGMMSLPKIKPIHMKITADDVVHEGDYLFGAVCNLFAFPGLLKLPAG
                     KVHTDDGLFEVILIKEPCSLIEWQAAINSVLSGKYDTSIVEFFQAGEMVIEPGENVAW
                     ALDGEYEGGSGGSIEVKNLPRKIGLIAGKRG"
BASE COUNT          384 a          491 c          561 g          401 t
ORIGIN      
        1 tgccctgcgg ctgcaggcgg cggtccgcgc ccgtataatg gtgacccagt tcttcggcca
       61 accaacatgc gcgttcggcc tgcgtgccgt cccgccgcag gatgatgtgc tcgccggttt
      121 cggttttaat gtacgcggcg gacatgccgg gaaccgggat ctcgcgctgc tgcacgtcga
      181 ttccgatttc gctggcttcg tcggtcaatt cttcgattcg atccatgttt ctgttcctca
      241 tcgggcggta cggattggct gaaaacggtt atccgttctc cttggagaac ttgtcggcca
      301 ggtattcgat gtattcttcg taacgctcgc ggccttccgg cgtgagctgc gccacgtcga
      361 aatgccccgc cgaggcgagg atgcgcgggg cgcgctcggt aacgctgtcg tccgcgagaa
      421 aatccagggt gacgtttaaa aaacgcgcca gcgcaagaag cgtggaacgc ttgacgtttt
      481 ccgcgccttt ttcatataag ctggcaatgg tcgtatacgg cacgccggac tgcgcggcga
      541 gctgggactt gttgagcccg cgttcgccaa gcagcaggtc gagcttttcg gtcagggtca
      601 agggatatca cctcaagctt gagtatatcg gaaatattac gacgtgtaaa caagaagtta
      661 cgatgcggcg taaatatttc agtcggattg agccgaattt gaactttcgg gtgtataata
      721 taatcatcaa gtaaagatta agaaccgtcc cgccgcaagg cggttgaacg gttccatcgt
      781 ttatctatga ggtagatact atgaacgcgc tgacggaact gaatttcgac aaaaaactga
      841 tgctcatcgt caaccctgtg gcgggacgga agcagtcgct ccggctcgtg ccggagatcg
      901 tgcgcatctt tatggacgcg gggtatctcg tgaccgtgat ggtgaccaag gcgcagggcg
      961 atgccacggc gtttgccgag catttcagct gcagttacga cgcagtggtt tgcgtgggcg
     1021 gggacggaac gctgagcgag acggccgcgg ggattgcgcg ctgcgagaaa gccgtgccgc
     1081 tcgggtatat tccggcgggc agcacaaacg tgttcgccac cgcgcacggg ctttccaacg
     1141 atatcctcac ggcggcgcga aacatcgcat ccgggggcgt taagtggatc gacacgggtt
     1201 tgttcaacgc gcggagcttt gcgttcatcg cggcgttcgg cgcgttctcc tggacgtcct
     1261 acgcgacccc gcagaacctc aagaacatcc tcggcacgcc cgcgtacttc tttgacggca
     1321 tgatgagcct gcccaaaatc aaaccgattc atatgaagat tacggcagac gacgttgtcc
     1381 acgaggggga ctacctcttc ggcgcggtct gcaacctctt tgcgtttccg gggttgctga
     1441 agctgcccgc gggcaaggtg catacggacg acgggctgtt tgaagtgatt ctcataaagg
     1501 agccgtgctc gctaattgaa tggcaggcgg cgatcaactc cgtgctcagc ggcaagtacg
     1561 ataccagcat cgtggagttc tttcaagcgg gcgaaatggt catcgagccg ggcgaaaacg
     1621 tcgcctgggc gctggacggc gagtatgaag gtggctccgg cgggagcatc gaggtgaaga
     1681 accttccccg aaagatcggg ctgatcgcgg gaaagagggg gtaaactata atattaacag
     1741 cagacgtttt gcagcgcttt cggacgaaag cgctctttac tatctttgtg accccaatgt
     1801 gaaaccgacg caaatccata cgccgctcct tgacaaa
//
LOCUS       DAOMGY010000374         1834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_73927_length_1834_cov_3.523890, whole genome shotgun sequence.
ACCESSION   DAOMGY010000374 DAOMGY010000000
VERSION     DAOMGY010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73927_length_1834_cov_3.523890"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PLR57_06210"
     CDS             <1..626
                     /locus_tag="PLR57_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ41087.1"
                     /translation="IEEMLKRLEDADCDCVATRRVTRKGEPKIRSAFARAFYHIINRI
                     SDTEFVDGARDFRMMRRSVVDAVLQLTEYHRFSKGLFMWVGFNTVWLEYENIERAAGE
                     TKWSFWKLAAYAVEGIVSFSTVPLRIATVTGCIVSAMALIYMVVRVVIAMIWGNPVAG
                     YPSLLAIMLCLGGFILLALGIIGEYVARTYMEAKRRPIYIQKESNIK"
     gene            complement(742..1488)
                     /locus_tag="PLR57_06215"
     CDS             complement(742..1488)
                     /locus_tag="PLR57_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQQ41088.1"
                     /translation="MNTRGICGTLLDMKTTATDYLMRDRLKNIDMLEIMSMPSARLHY
                     AGADGVLLEHDDLFLLSANDGTAEKFLPVLLSVLPESRPSLVILHSDELLQPLKEAHG
                     YRTIMDCCHAVYDRKEPISYSLPAGAEIRRLDESHIDFVHEHYHTVDDIDYIRERIEE
                     GMFGAFIGGDCVGFVGTHDELSIGLLFVLPACRRLGLAFALEAAITNHLLSIGRLPFC
                     QIQLQNAPSLALQKKLGFTVSDNPIYWLER"
     gene            1699..>1834
                     /locus_tag="PLR57_06220"
     CDS             1699..>1834
                     /locus_tag="PLR57_06220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ41089.1"
                     /translation="MADVTAIVLTLNEEKNIESCIRSVAGFCKRVVIVDSGSTDRTLE
                     I"
BASE COUNT          418 a          466 c          534 g          416 t
ORIGIN      
        1 tgatcgagga gatgctcaag cgcctcgaag acgcggactg cgactgcgtt gccacccgcc
       61 gcgtcacccg caagggcgag ccgaaaattc gcagcgcgtt tgcgcgggcg ttctaccata
      121 ttattaaccg catttcggat acggaattcg tggacggcgc acgggatttc cgcatgatgc
      181 ggcgcagcgt ggtggatgcc gttctgcagc ttaccgagta ccatcgcttc tccaaaggcc
      241 tgttcatgtg ggtcggtttc aacaccgtct ggctggaata cgaaaacatc gagcgcgcgg
      301 caggggagac caagtggtcg ttttggaagc tcgcggccta cgccgtggaa ggcatcgttt
      361 cgttctccac cgtgccgctg cgcattgcaa ccgtgaccgg ctgcatcgtt tccgctatgg
      421 cgctgatcta tatggtcgtt cgggtcgtca ttgcaatgat ctggggcaac cccgttgcgg
      481 gctatccgtc cttgctggcg attatgctgt gcctcggcgg gttcattctg ctcgcgctcg
      541 gcattatcgg cgagtacgtc gcgcgtacat atatggaagc caagcgcagg ccgatttata
      601 ttcagaaaga aagtaatata aagtagagct tgcttttctt taaaagaaaa gcaacaaaag
      661 aacctctgta tgattcagag gttcttaatt tattagataa aagtcttttt gttacttttt
      721 cttcagaaaa agtaaacgtt cctatctctc aagccaataa ataggattat cggaaaccgt
      781 gaagccaagc tttttctgca gcgcaagcga tggcgcgttt tgcagctgta tctggcagaa
      841 cggcagccgc ccgatggaga gcagatggtt cgttatggcc gcttccagag cgaacgcgag
      901 gccgagtctg cggcaggcgg gcaatacgaa taataaacca atggagagct cgtcgtgcgt
      961 gccgacaaac cctacgcagt cgccgcctat gaacgcgccg aacatgcctt cctcaatgcg
     1021 ctcgcggatg tagtcgatgt cgtccacggt gtggtagtgc tcgtgcacaa aatcgatgtg
     1081 gctttcgtcg agccgccgga tttccgctcc tgcgggcagg gaataggaga tcggttcctt
     1141 gcggtcgtaa acggcgtggc agcagtccat aatggtgcga taaccgtgcg cttctttgag
     1201 cggctgcaag agctcgtcgc tgtgcagaat gaccagcgaa ggcctgcttt ccggcaaaac
     1261 ggagagcaga accggcagga atttctccgc cgtgccgtcg tttgcggaga gcagaaacag
     1321 gtcgtcgtgc tcaagcagca caccgtcagc ccccgcgtaa tgcaggcgcg cggaaggcat
     1381 ggacatgatt tccagcatgt cgatgttctt gagccggtcg cgcattaaat aatctgttgc
     1441 ggtcgttttc atgtcgagca gtgtaccaca gattccgcgt gtgttcaacc gtaaattccg
     1501 cgcggcaact gtcggaaaca agcaggagat tccaaggctt ttccggcgga agagagttgt
     1561 aaatcttacg tgaagaaacg cgtcaacgcg cccgccgcgt ttgacatcgc agggagcgga
     1621 atggtacact acgataggta tattacagga tgaaacaggg gtaaattttg ccccgatgac
     1681 gaaagggaaa atcgctacat ggcggacgtt acggcgattg ttctgaccct caacgaagag
     1741 aagaatattg aaagctgcat ccgttcggtg gcggggttct gcaaacgcgt tgtcatcgtt
     1801 gacagcggca gtacggaccg cacgctggag atcg
//
LOCUS       DAOMGY010000375         1833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_73998_length_1833_cov_3.400450, whole genome shotgun sequence.
ACCESSION   DAOMGY010000375 DAOMGY010000000
VERSION     DAOMGY010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73998_length_1833_cov_3.400450"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /gene="gguA"
                     /locus_tag="PLR57_06225"
     CDS             complement(<1..1363)
                     /gene="gguA"
                     /locus_tag="PLR57_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQQ41090.1"
                     /translation="MTDNILEMKHITKEFPGVKALDDVSLSVQRGEIHAVVGENGAGK
                     STLMNVLSGIYPHGSYEGEIYYDGKPCRFSKIKDSENCGIVIIHQELALVPYLTIAEN
                     MFLGNERGSSFAIDWDETFAKADELLQVVGLMESSHTLIKDIGVGKQQLVEIAKALSK
                     NVKLLILDEPTASLNESDSMKLLNLLKEFKKKGVTSIIISHKLNEIAYVADRITIIRD
                     GAVIETLDMAEGKVDEARIIRGMVGRELTNRFPAREHHVQDEVVLEVEHWSVKHALYT
                     DRTVVDDVSFHVKRGEVVGIAGLMGAGRTELAMSIFGRSYGSEITGTLKIHGKKVVLN
                     NEHDAIRHGLAYVTEDRKGNGLILSNPIRVNTTLARLDKVSRRRVIDLDKEYKMAVSM
                     KDLLGIKCPSVEQVVGNLSGGNQQKVLLAKWMFAEPDILLLDEPTRGIDVGAKYEIYC
                     IIND"
     gene            complement(1587..>1833)
                     /locus_tag="PLR57_06230"
     CDS             complement(1587..>1833)
                     /locus_tag="PLR57_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ41091.1"
                     /translation="DIIAGKQSMSIFKDTRTLAAQTVTMVSDILAGKTPETNDNKSYD
                     NGTGIIPTYLCAPVFADINNYEELLVDSGYYKADELK"
BASE COUNT          346 a          507 c          529 g          451 t
ORIGIN      
        1 ggtcgttgat gatgcaatag atttcgtatt ttgcgccgac gtcgatgccg cgcgtcggct
       61 cgtccagcag caggatatcc ggttcggcga acatccactt ggccagaagc accttttgct
      121 ggttgccgcc ggagaggttg cccacgacct gctccacgct gggacacttg atgcccaaaa
      181 ggtctttcat ggaaacggcc atcttgtatt ccttgtcgag gtcgatcacc cggcggcggc
      241 tgaccttgtc gagccgcgcg agcgtcgtgt tgacgcggat ggggttggag aggatcaggc
      301 cgttgccctt gcggtcctcc gttacgtacg ccagcccgtg ccggatggcg tcgtgttcgt
      361 tgttcagcac gacctttttg ccgtgaattt tcagcgtgcc ggtgatttcc gagccgtagc
      421 ttctgccaaa gatgctcatt gcgagctctg tgcgccccgc gcccatcagc cccgcaatgc
      481 cgaccacttc gccgcgcttg acatggaacg aaacgtcgtc cacgaccgtg cggtcggtgt
      541 agagcgcatg cttaacgctc cagtgctcga cttccagcac gacttcgtcc tgcacgtggt
      601 gttcgcgtgc ggggaagcgg ttggtcagct cgcggccgac catgccgcga atgatgcgcg
      661 cttcgtccac cttgccttcc gccatatcga gcgtttctat caccgcgccg tcgcggatga
      721 tggtgatgcg gtcggcgacg tacgcgattt cgttgagctt gtgcgaaatg ataatcgacg
      781 tgacgccctt tttcttgaac tccttgagca ggttcaacag cttcattgaa tcgctctcgt
      841 tgagggaagc cgtcggctcg tccagaatga ggagcttgac gtttttactg agcgctttgg
      901 caatttccac gagctgctgc ttgcctacgc cgatatcctt gatcagcgtg tgggaagact
      961 ccataagccc gaccacctgc agcaattcgt ccgctttggc gaacgtttcg tcccagtcta
     1021 tggcaaatga actgccgcgt tcgttgccga ggaacatgtt ttccgcaatg gtgaggtagg
     1081 gaaccagagc gagttcctga tggataatga cgataccgca gttttcactg tccttaatct
     1141 ttgaaaagcg gcagggcttg ccgtcatagt agatttcccc ttcgtagctg ccgtgcgggt
     1201 agataccgga gaggacgttc atcagcgtgg atttgccagc gccgttttcg ccgaccaccg
     1261 cgtggatttc tccgcgctgc acgctcaggc ttacgtcgtc cagcgccttg acgccgggga
     1321 actcctttgt aatatgcttc atttccagaa tgttatccgt catagcaatc ctccaagaat
     1381 attgactccc gtgtaagcga acagcgatca gggcgggaac gaaaagaacg agagatgaac
     1441 gctgtaaaaa aaagaaccgt tctgtgcctt gacggcgcga acgtttcgct gtaccaaaag
     1501 attctcttga tgtaccgaaa ccttcgcgaa gaccgcaggc gggcggttgc ccacccgcct
     1561 gcgatggatg ctgaagagta ttggaactat ttgagttcgt ccgctttgta gtagccggaa
     1621 tcaaccagca gttcttcgta gttgttgata tccgcaaaca ccggtgcgca gaggtaggtc
     1681 gggatgatgc ccgtgccatt gtcgtagctc ttgttgtcgt tggtttccgg cgtcttgccc
     1741 gcgaggatgt cgctgaccat ggtcacggtc tgggcggcaa gcgtacgggt atccttgaag
     1801 atggacatcg actgtttgcc ggcgatgatg tcc
//
LOCUS       DAOMGY010000376         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_74057_length_1832_cov_3.550366, whole genome shotgun sequence.
ACCESSION   DAOMGY010000376 DAOMGY010000000
VERSION     DAOMGY010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74057_length_1832_cov_3.550366"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /gene="plsX"
                     /locus_tag="PLR57_06235"
     CDS             <1..976
                     /gene="plsX"
                     /locus_tag="PLR57_06235"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HQQ41092.1"
                     /translation="NAPQAVIEGVQLVLPELPADAELLLFGDENVLGSDIADAHINDP
                     RVKIVHTSQVIGCDEQPTLAIRKKRDSSMVRAVEAVANGEADCVLSAGSTGALLTGGT
                     LIIKRLKGVKRPALATIMPTPESCVLLLDCGANTDCKSEYLVQFALMGSAYMKSVMGV
                     ENPRVGLLSNGAEEEKGNELTKETHQMLKTAPVNFVGNCEARDVLGGEFDVVVCDGFD
                     GNVVLKGTEGAAALILDMLKKALMSSWVSKIGALLCKPAFRILKKKLDYTEYGGAPLL
                     GVNGGVIKAHGSSNGKAFRSAILQGVKLIDSNVTALLGAEIAAMGVEE"
     gene            1117..1341
                     /gene="acpP"
                     /locus_tag="PLR57_06240"
     CDS             1117..1341
                     /gene="acpP"
                     /locus_tag="PLR57_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQQ41093.1"
                     /translation="MYFEKVRDIIAKQLDIEAGTITMTSRLIDDLKADSLDIVELIMD
                     LEQEFNIEIPDEELPKVQTVADIVGYLDKK"
     gene            1482..>1832
                     /locus_tag="PLR57_06245"
     CDS             1482..>1832
                     /locus_tag="PLR57_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HQQ41094.1"
                     /translation="MPVLENTLSVLEENLRYTFLNRSLLETALTHTSFVKGDGKHQTH
                     NERLEFLGDAVLELCVSEHLYRDHPEMQEGAMTRHRARLVCEKALFSAAVALGLPQSL
                     RLGNGEELTGGREKP"
BASE COUNT          437 a          495 c          552 g          348 t
ORIGIN      
        1 caacgcgccg caggcggtca tcgagggggt gcagctcgtt ttgccggagc ttccggcgga
       61 tgcggagctg ctgctctttg gcgatgagaa tgtgctgggc agcgacattg cggacgcgca
      121 tatcaacgac ccgcgggtaa aaattgtcca tacctcgcag gtgatcggct gcgacgaaca
      181 gccgacgctc gccatccgca aaaagcgcga ctcttccatg gtgcgcgccg tcgaagcggt
      241 ggcaaacggg gaagcggact gcgtgctttc tgcgggcagc acgggcgcgc tgcttaccgg
      301 cggcacgctg attatcaagc gcctcaaagg ggttaagcgc cctgcgctgg caaccataat
      361 gcctacgccg gagagctgcg tgctcttatt ggactgcggg gcaaacacgg actgcaagag
      421 cgaatacctc gtgcagtttg cgctcatggg ctcggcttac atgaagagcg tcatgggcgt
      481 ggaaaatccg cgcgtaggcc ttctcagcaa cggcgcggaa gaggaaaagg gaaacgaact
      541 gaccaaggaa acacaccaga tgctcaaaac ggctcctgtc aactttgtcg gcaactgcga
      601 agcgcgggat gtgctgggcg gtgaattcga cgtggtcgtc tgcgacgggt tcgacggcaa
      661 cgtcgtgctc aaaggtacgg aaggcgcggc ggcgctgatt ctcgacatgc tcaaaaaagc
      721 gctcatgtca agctgggttt ccaaaatcgg cgcgctgctc tgcaagccgg cgttccgtat
      781 cctcaaaaag aagctcgact ataccgagta cggcggcgcg ccgctgctcg gcgtcaacgg
      841 cggcgtgatc aaggcgcacg gcagctccaa cggcaaggcg ttccgctcgg ccatcctgca
      901 aggcgttaaa ctgatcgatt ccaacgtgac cgcgcttctc ggcgcggaga tcgccgcgat
      961 gggcgttgag gagtaagcag acagaaattt tacgttgcac ggcggggatg aaaatcacgc
     1021 tcacacagtt ttcgtgagga ttgacagatc gacccgcgta ttatagaata ctacccgaat
     1081 gggatgcgga tgtcgcattg aaaaggagtt atcaatatgt attttgagaa agttcgagat
     1141 attatcgcaa aacagttgga tattgaagcc ggaacgatta cgatgaccag ccgcctgatc
     1201 gacgacctca aagcggattc gctcgacatc gttgaactga tcatggacct cgaacaggaa
     1261 ttcaatatcg aaatccccga tgaggaactg cccaaggttc agaccgtcgc cgacatcgtt
     1321 ggctacctgg acaagaagta agaaaagacc gcagcatccg aatgcccgca tccgacggtg
     1381 aaccagggtg cgggcttttt tgtaaagcat tccccgcctg aaaaaccacc tacaaccaaa
     1441 gaaacagact tactattttt gccgcttcaa aggaagattg catgccggta cttgaaaaca
     1501 cgctttccgt tttggaagaa aacctgcggt atacgtttct caaccgttcc ctgctggaaa
     1561 cggcgctgac gcatacctcg tttgtcaaag gagacggcaa gcaccagacg cataacgaac
     1621 ggctggagtt tttgggcgac gcggtgcttg agctttgcgt cagcgagcac ctctataggg
     1681 atcatcccga aatgcaggaa ggcgcaatga cgcggcaccg cgcgcggctc gtctgcgaaa
     1741 aggcgctgtt ttccgcggcg gtagcgctgg ggctgccgca gagtctccgg ctcggcaacg
     1801 gcgaggagct caccggcgga cgcgagaagc ct
//
LOCUS       DAOMGY010000377         1827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_74352_length_1827_cov_3.652370, whole genome shotgun sequence.
ACCESSION   DAOMGY010000377 DAOMGY010000000
VERSION     DAOMGY010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74352_length_1827_cov_3.652370"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PLR57_06250"
     CDS             complement(<1..758)
                     /locus_tag="PLR57_06250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQQ41095.1"
                     /translation="MQRSESIKQRISSILLDDDQRYRFQFLCVYGLLALISGGMTILN
                     TITHKGALTWSTLIFSVLCAVVFTLLYYKKIPLSVSKGIFVVSFYLLLTFFVVTGNPD
                     GFSILWVCLLPACGLLTFGRKGGTLLCLGMLVILLFFLETPWGLNLLMYEYSPTFRMR
                     FPLLYVAFYFTSLLLESIRMLTHKKLVETQELYRYLYAHDALTKLYNRHGFNERMDKF
                     FTAKQRNLALIILDIDPFKAVNDLHGHLAGDDVL"
     gene            1057..>1827
                     /locus_tag="PLR57_06255"
     CDS             1057..>1827
                     /locus_tag="PLR57_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41096.1"
                     /translation="MEHLYISDMTLRLEAKRTEKLLSFREKLEIARSLDRLRLYGVEL
                     WEIQNEKADVLLIKSIAAAVKESRLVMPCSLTKEGVTLAWSAVKEAAKPCLLISAPTS
                     TAQMEYICHKKPDQMLALIGELVAYAKSLCPEVEFCAEDATRSDKDFLYRALETAAAA
                     GADAITLCDGSDGMLPEEFGRFVADAVKNAPSLKSKLVGVQCTDALSLSAACAVSAIA
                     AGARLIKTSAGDDGQLSLETFADVLISLAPAAIALTAQA"
BASE COUNT          460 a          465 c          523 g          379 t
ORIGIN      
        1 tgcagcacat cgtctccggc cagatgtccg tgcagatcgt tgaccgcttt gaaagggtcg
       61 atatcgagga tgatcagcgc gagattccgc tgcttagccg taaagaattt gtccatacgc
      121 tcattgaagc cgtggcggtt atagagcttg gtgagcgcgt cgtgcgcgta gagataacgg
      181 tacagctcct gcgtttcgac gagcttttta tgcgtgagca tacggatgct ctccagcagc
      241 agcgaggtga agtagaacgc aacgtatagc agaggaaagc gcatacggaa tgttggcgaa
      301 tactcgtaca tcaggagatt gagcccccac ggcgtttcga gaaaaaacag gaggattacc
      361 agcatgccga ggcagagaag cgttccgcct ttgcggccga acgtcaacag cccgcaggcg
      421 ggcaaaagac agacccatag gatgctgaaa ccgtcgggat tgccggtaac gacaaagaac
      481 gtcagcagca gatagaaact gacgacgaag attcccttcg atacgctgag cgggattttt
      541 ttgtaataaa ggagcgtaaa aacaaccgcg cagagaacgg aaaagatcag cgtggaccat
      601 gtgagcgcgc ctttatgcgt tatcgtgttg agaatcgtca tgccaccgga gatcagggca
      661 agcagtccgt aaacgcaaag aaactggaat cgataacgtt gatcatcgtc cagcaaaatg
      721 gaagaaattc gttgtttgat cgattcggag cgctgcatat tcgtattata ctgtggattt
      781 ttgtgccgcg caatcgagct gtgccgaaaa aacattcatc ggcaaaaagg cgattaatac
      841 gtgaaaggag cttcggtttt gtatatcata tattgacatt ttgccaaggc ctgtgatacc
      901 atccaacaaa tcccttttgc caaatcaagt ttcgcttatt caacaagcga actgcttttc
      961 atcagatcct gacggcacgt ccttaaagcc gccgttttcg acggactttg gggatatttt
     1021 tttaaacgcc ggtcgaggat cggaaggaga aacgccatgg aacacctgta catttccgat
     1081 atgaccctgc ggctggaagc aaagcgcacc gaaaaactgc tcagcttccg cgaaaaactg
     1141 gagatcgcgc gctcgctgga tcgattgcga ctgtacggcg tggagctatg ggagatccag
     1201 aacgaaaaag ccgacgtact gctgataaaa tcgatcgccg ccgccgtcaa ggagagcagg
     1261 cttgtcatgc cctgctcgct cacaaaggaa ggcgttactt tggcctggag cgccgtaaaa
     1321 gaagcggcga agccctgtct gttgatttct gcgccgactt ccacagcgca gatggagtac
     1381 atctgccaca agaagccgga tcagatgctc gcgcttatcg gcgagctggt tgcgtacgca
     1441 aaatcgctct gccccgaagt ggagttctgc gccgaggacg caacccgaag cgacaaggac
     1501 tttttatacc gcgcgctgga aacggctgct gcggcgggcg ctgacgcaat caccctctgc
     1561 gacggttcgg acggcatgct gccggaagag ttcggccggt ttgttgctga cgcagtaaaa
     1621 aacgcgcctt cgctgaagag caagctcgtc ggcgtgcagt gcacggacgc gctgagcctt
     1681 tccgccgcct gcgcagtcag cgcaatcgcc gccggagcga ggctgattaa aacgtccgcg
     1741 ggcgacgacg gacagctctc gctggagacg ttcgcggacg ttttaatcag cctcgctccg
     1801 gcggcgattg cgctgactgc gcaggcg
//
LOCUS       DAOMGY010000378         1827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_74373_length_1827_cov_2.655756, whole genome shotgun sequence.
ACCESSION   DAOMGY010000378 DAOMGY010000000
VERSION     DAOMGY010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74373_length_1827_cov_2.655756"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..649
                     /locus_tag="PLR57_06260"
     CDS             209..649
                     /locus_tag="PLR57_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQQ41097.1"
                     /translation="MEHIEASVPNETQTSRQSNRTTGNRTRKRVWLWVLAALTGLYLM
                     VDWFGKSRFIVQNYVIEDERIIDDVRIVVVSDLHSALYGADQSDLIDAIAQADPAVVL
                     LDGDIFHHHGSNKHAVTLLNNSRTVFNATSSWATTNTRQSKLPG"
     gene            607..1107
                     /locus_tag="PLR57_06265"
     CDS             607..1107
                     /locus_tag="PLR57_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41098.1"
                     /translation="MGNHEYQTKQITWVKRQVEEAGIRILDGESVDVTAGKTTVRLYG
                     IDDGHSGKARQLAELARAGKERDDSVYSILAIHVPNDVKNILPLGFDLMLSGHTHGGQ
                     IRIPGVLNGLYAPGQGMFPTYGGGRYDFSKQTLIISRGLAKVPYWLPRVFNPPELVVV
                     TLTATK"
     gene            complement(1181..1720)
                     /locus_tag="PLR57_06270"
     CDS             complement(1181..1720)
                     /locus_tag="PLR57_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQQ41099.1"
                     /translation="MSNYLDLVKRRESCRNFDPNRPVEKEKLALCAEAAWLAPSACNS
                     QPWRYLIVTNPELVQKLRPLMQGLGMNKFVNQCPAFVVVLEEPTKLKVSVSSKFKDQS
                     FAPIDVGLSTSQFCYEATELGLSTCIIGWHNEPKIKELFEIDSVSRVRLVLCVGYAAS
                     DELRKKQRKPIDDVVKYFE"
BASE COUNT          395 a          515 c          505 g          412 t
ORIGIN      
        1 caccattggc tacgtgtaca gcaccttcgg cagcctcagc taacccgata cacatcctgt
       61 catacaagag agccgtcctt caacgggcgg ctctctttat tattcgcctg ttgccacgct
      121 ttaaattaca agtccgcaat gtgtcctttt ggtggaatct cctctgcttc ccttttctcc
      181 tatgctctgc tttgctatac tgaacagcat ggaacacatc gaagcaagcg ttccaaacga
      241 aacgcaaaca tccagacaat cgaatcgaac gaccggcaat cgcacccgaa aacgagtttg
      301 gctttgggtg ctcgccgcgc tgacggggtt gtatctgatg gtggactggt tcggcaaaag
      361 ccgctttatc gtgcagaatt acgtcatcga ggacgaacgg attatcgacg acgtccgcat
      421 tgttgtggtg tccgatctgc attccgcgct ctacggggcg gatcaaagtg atttgatcga
      481 cgccatcgcg caggcagatc ccgccgtggt gctgctggac ggagatattt ttcaccacca
      541 tggcagcaac aaacacgccg tcacgctgct caacaactcg cgaaccgttt tcaatgctac
      601 ttcatcttgg gcaaccacga ataccagaca aagcaaatta cctgggtaaa gcggcaggta
      661 gaagaagcgg gcatccgcat tctcgacggc gaatcggtgg atgtcacggc gggcaaaacc
      721 acagtgcgct tgtacggcat tgacgacggg cacagcggaa aagcccggca gctggcggag
      781 ctggcgcgcg ccggcaagga gcgcgacgac agcgtatact ccattctcgc catccatgtg
      841 ccgaacgacg taaagaatat tctcccgctg gggttcgacc tcatgctctc cggccatacc
      901 cacggcgggc agattcgtat tcccggcgta ctcaacggtc tgtacgcgcc cgggcaggga
      961 atgttcccca cgtacggcgg cggacgatac gactttagca aacaaacgct catcatcagc
     1021 cgcggtctcg ccaaagtccc ctactggctg ccgcgcgtgt tcaacccgcc ggagctggtt
     1081 gtcgtcacgc tgacagcaac gaagtagctt ttgaacaaga ctttaatccg tagaaacgca
     1141 aacggcgcgc cggaaggcgc gccgctgttc gtttagctcg ttattcgaaa tacttcacca
     1201 cgtcgtcgat gggcttgcgc tgctttttac ggagttcgtc gcttgccgcg tatcccacgc
     1261 agagcacgag gcgcacgcgg gagacgctgt caatttcgaa cagctccttg atcttgggtt
     1321 cgttgtgcca gccgatgatg caggtggaaa gccccagttc ggtggcttca tagcagaact
     1381 gtgaagtgga gaggccaacg tcgataggcg cgaagctctg atccttgaac ttggaggaga
     1441 cggaaacctt cagtttggtc ggctcttcga ggacgacgac gaacgccggg cattggttga
     1501 cgaatttgtt catgccgagc ccctgcatga gcggacggag cttttgaacg agttccgggt
     1561 tcgtcacaat caggtaacgc cagggctggc tgttgcaggc ggaaggcgca agccaggccg
     1621 cttcggcgca gagcgcgagc ttttcctttt ccacggggcg gttggggtcg aagttgcggc
     1681 agctttcgcg gcgcttgacc aagtccaaat aattactcat gacgatttct cctttggttc
     1741 atattgatcg gctctatttt ccggcgattt tccttcgcgt gtcttcctgc atgagttcgc
     1801 gcagttcctt aagatatgga aactccg
//
LOCUS       DAOMGY010000379         1824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_74538_length_1824_cov_3.664782, whole genome shotgun sequence.
ACCESSION   DAOMGY010000379 DAOMGY010000000
VERSION     DAOMGY010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74538_length_1824_cov_3.664782"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLR57_06275"
     CDS             <1..291
                     /locus_tag="PLR57_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41100.1"
                     /translation="VVKINDCRWTPMVCTLHILHKLVSMALSVYMLTRPYIFSAEFTA
                     FMKNFNVQELWGFPWFGVNGMNPFLIFIIVVMVIGTLADCIKAITLTVKDRK"
     gene            complement(338..1060)
                     /locus_tag="PLR57_06280"
     CDS             complement(338..1060)
                     /locus_tag="PLR57_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQQ41101.1"
                     /translation="MEIRVHEIGSYVVRNYVLETPAGWIVLDTGYRDDEAVFCERFEK
                     LAPLDQIKYIFLTHAHDDHAGFLSSLLGRTNAALVLHESAVPQLAMGKNATPPGAGYS
                     SRLASSFALVKKEFGFPAVIPDEHAIVLHSEADQPFLALGLPLRVVFLPGHTADSIAL
                     FDEETRFLYCGDAAMNAVISRARHTIWIEDPAEFGRSWDKMIALEPRMIFPSHGNPFL
                     PKDLVKYRHFMDRRKLIQPKAR"
     gene            1167..1796
                     /locus_tag="PLR57_06285"
     CDS             1167..1796
                     /locus_tag="PLR57_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41102.1"
                     /translation="MKKKTLVIIMLLTMVAPFVLYSCLDRIRDYIPFTGPDKKSVEHF
                     EEAFNEQMAQYGMSIDIYSARWHENEGTLHKTVPIQCEDGSDITCTYYPIAKSPKSQI
                     RVIEFEQSLNSNDPQTIYIIPLMEFILQEFEAPMLEDKDKAFDAPDAVSYNEAIRNCQ
                     TFVAGEQKELEFYVSSVDHDSSPVFLTRETGEKNSIKIRIFIFSGQRKW"
BASE COUNT          456 a          452 c          473 g          443 t
ORIGIN      
        1 gtcgtcaaga tcaacgactg ccgctggacg cctatggtct gcacgctgca cattctgcat
       61 aagctcgtga gcatggcgct ttcggtctat atgctgacgc gcccgtacat cttcagcgcg
      121 gagttcacgg cgtttatgaa gaacttcaac gttcaggagc tttggggctt cccgtggttc
      181 ggggttaacg gcatgaatcc gttcctgatt ttcattattg tcgtaatggt catcggcacg
      241 cttgcggact gcatcaaagc aatcacgctt acggtgaagg acagaaagta acgaatcgca
      301 ataaacaaaa aaggacgcat atgcgtcctt ttttgtgtta tctcgctttt ggctgaatta
      361 gcttgcgccg atccataaag tggcggtatt tgaccaaatc tttcggtaaa aacggattcc
      421 catgcgaggg gaagatcatg cgcggctcaa gcgcaatcat cttatcccaa ctgcgaccaa
      481 actccgccgg atcctcgatc cagatggtat gccgcgcacg gctgattacg gcgttcattg
      541 ccgcgtcgcc gcagtagaga aagcgcgttt cctcgtcgaa cagcgcaatg gaatcggctg
      601 tgtgcccggg caggaatacg accctcagcg gcagtccaag cgcgaggaac ggctgatccg
      661 cctcgctgtg cagaacgatg gcgtgctcgt ccggtatgac tgctgggaag ccgaactcct
      721 tttttactag cgcgaacgag gaggccaggc gcgaggaata gccggctccc ggaggcgttg
      781 cgtttttgcc catggcaagc tggggcacgg cggattcgtg gagcaccaaa gcggcgttcg
      841 tcctgccaag aaggctcgat aaaaaccccg cgtggtcgtc gtgtgcatgg gtcaaaaaga
      901 tgtatttgat ttgatcgagc ggcgcgagct tttcgaaccg ctcgcagaac accgcttcgt
      961 cgtcgcgata gccggtgtcg agaacgatcc agcctgcggg cgtttccagt acgtaatttc
     1021 tcacgacata gctgccgatt tcgtgtacgc gaatttccat gcgttctacc tccagtttcg
     1081 acaatatcat accacatttg tcatttacgt acgagtgttc gcgtggtaaa atacccattg
     1141 tatatacgtg ctcggggagt gaaacgatga aaaagaaaac gctggtaatc atcatgctgc
     1201 tgaccatggt tgcgcctttt gtcctgtatt cttgcttgga tcgaatcaga gactatattc
     1261 cgttcacggg gcctgataaa aaatcggttg aacactttga agaggcgttc aacgagcaaa
     1321 tggcgcagta tggaatgtcc atcgatatat acagcgctcg ttggcatgaa aacgagggga
     1381 ctctccacaa gactgttccg atccaatgcg aggatggaag cgacatcact tgcacatact
     1441 atccaatcgc taaaagccca aaatcacaga tcagagtaat tgaattcgag caatcgctga
     1501 acagcaatga cccgcagacg atctatatca tccctttgat ggagttcata ctgcaggaat
     1561 tcgaagcgcc catgttggaa gataaagaca aggcgttcga tgctccggat gctgtttcct
     1621 acaacgaagc aattcgtaac tgccagactt ttgttgccgg tgagcaaaaa gagcttgaat
     1681 tttacgtttc atcggttgac catgattcct ctcctgtatt cctgacgcgc gagaccggag
     1741 agaaaaatag cataaaaatc cggatcttca tattttccgg tcaaagaaaa tggtgagata
     1801 gtagtttgct attaaccatc gcca
//
LOCUS       DAOMGY010000380         1823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_74609_length_1823_cov_3.605769, whole genome shotgun sequence.
ACCESSION   DAOMGY010000380 DAOMGY010000000
VERSION     DAOMGY010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74609_length_1823_cov_3.605769"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PLR57_06290"
     CDS             complement(<1..1206)
                     /locus_tag="PLR57_06290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQQ41103.1"
                     /translation="MLKIAQCTDSFLPVSDGVGRVAHAYARALAQRGNDVTVITPMVE
                     NGFRGRHTFEILDFLALPVPGSNRKTGVAALDLHYLTRVNAMRFDVIHTHSTGSAGME
                     AVRLADRLRVPLVGTFHPKYIREYLTSARNERDSLLTKLFAFDYFSRCDEIWTANEEA
                     RELLLERGFQGNIEVFDNGTELEPVGGETRQRAREAFHLTNAPVLLFAGSLERQKNLP
                     RVLEAAAILKRRNVVFQLLVAGRGPDEAHIRERIRELNLNGTVRLLGYITDESLLGGL
                     YAESALCLFPAQQISAGFVVREAAAQGTPSLVIAGSAPSSLIENGVNGLTCADTPESM
                     AEAIEGYLAAPERMRQLSENARALPSVSWDAVMNRVEERYKELASLERMQLSRKGACS
                     EKRWKRSIFR"
     gene            1369..>1823
                     /locus_tag="PLR57_06295"
     CDS             1369..>1823
                     /locus_tag="PLR57_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41104.1"
                     /translation="MDATGSTQISMQDYFATPKSRKITAVILAAIVLAMILGAVLASQ
                     KPAVEPVQLSHALDGTSTYTYLDFQLLSEWMYKVTGDSNYTYYEAMDPDENWYIVALN
                     DDLVEQLKPYQDAYSYFYVDGAPYAEIPEPYRITGMTYPLSSDDISALAS"
BASE COUNT          375 a          584 c          483 g          381 t
ORIGIN      
        1 gcgaaagatc gaccttttcc atctcttttc ggaacacgcc cccttgcggc tgagctgcat
       61 gcgctccaga gaggcaagct ctttgtaccg ttcctcaacg cggttcatca ccgcgtccca
      121 ggaaacggaa ggcagcgcgc gcgcgttttc gctgagctgg cgcatccgtt ccggcgcggc
      181 gaggtaccct tctatcgctt ccgccatgct ctccggcgta tccgcgcagg tcagtccgtt
      241 gacgccgttt tcgatcaggc tgctcggcgc gctgcctgca atcaccagcg aaggcgtgcc
      301 ctgcgccgcg gcttcccgca ccacgaaccc cgcgctgatc tgctgcgcag gaaacaggca
      361 cagcgcgctc tcggcataca gcccgccgag aagcgattcg tccgtaatat acccgagcag
      421 ccgcacggtt ccgttcaggt tcaattcgcg gatgcgctcg cggatatgcg cttcatccgg
      481 cccgcgcccc gcaaccagca gctgaaaaac aacgttccgg cgtttgagaa tagccgccgc
      541 ttccagcacg cgcggcaggt tcttctgccg ctccagactg ccggcaaaca gcagcacggg
      601 cgcgttcgtg aggtgaaagg cttctctcgc gcgctggcgc gtttcgccgc caacgggctc
      661 cagctccgtg ccgttgtcaa acacctcgat attgccctga aagccgcgtt ccagcagcag
      721 ttcccgcgct tcctcgtttg ccgtccagat ttcgtcgcag cggctgaaat agtcaaacgc
      781 aaacagcttg gtcaaaagcg aatcgcgctc gtttctggcg ctggtcaaat actctcgaat
      841 gtacttggga tgaaacgtcc ccaccaacgg aacgcgcaga cggtcggcaa ggcgcaccgc
      901 ctccatgccc gcggaacccg tgctgtgggt atgaatcacg tcgaagcgca tggcgttcac
      961 gcgggtcagg taatgcaagt ccagcgcggc aacgcccgtc ttcctgttgg aaccgggcac
     1021 gggcagcgcc agaaagtcca gtatttcaaa cgtgtgcctg ccgcgaaatc cgttctcaac
     1081 catcggggtg atcacggtaa cgtcgtttcc ccgctgggca agcgcgcgcg cataagcgtg
     1141 cgcaacgcgg ccgaccccgt cggatacagg cagaaaagag tctgtacatt gtgcgatttt
     1201 cagcatagat gcaatccatc ctccgccgtc attttaccat acttgagcgt tgaatgatat
     1261 tcccctgccc acctgccgaa actgacgtta accgcctgcc ggaaatgttt ttgacgcgtc
     1321 cgttcgctcc gattataatg agtcatacga tgatcggggg gactataaat ggacgctaca
     1381 ggttccacac agatttccat gcaggactac tttgcaacgc ccaagagcag aaaaatcaca
     1441 gccgtcattc tggcggccat cgttcttgct atgattctcg gcgcggtgct cgcttctcaa
     1501 aaacccgccg tcgaaccggt gcagctttcg cacgcgctcg acggaaccag cacctatacc
     1561 tacctggact ttcagttgtt gtccgaatgg atgtacaaag tcacggggga ttccaactac
     1621 acctactatg aggccatgga tccggatgaa aactggtata tcgttgcgct gaacgacgac
     1681 cttgttgaac agctcaaacc gtatcaggac gcttacagct acttctatgt cgatggcgcg
     1741 ccgtatgccg aaataccgga accctaccgt atcaccggca tgacgtaccc actttcttcg
     1801 gacgatatct ccgcgctcgc ttc
//
LOCUS       DAOMGY010000381         1812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_75292_length_1812_cov_2.217985, whole genome shotgun sequence.
ACCESSION   DAOMGY010000381 DAOMGY010000000
VERSION     DAOMGY010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75292_length_1812_cov_2.217985"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1313
                     /locus_tag="PLR57_06300"
                     /pseudo
     CDS             147..1313
                     /locus_tag="PLR57_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
     gene            1310..>1812
                     /gene="coaE"
                     /locus_tag="PLR57_06305"
     CDS             1310..>1812
                     /gene="coaE"
                     /locus_tag="PLR57_06305"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQQ41105.1"
                     /translation="MSKRAYKRIGLTGGIGAGKSTAAKRFRELGVLVLDADEISRASL
                     KKGGACYDGVVALFGSEILAQDGEIDRKKLAGIVFADEEKRRQLNAIVHPYVIETMFS
                     RAEETLNKSGGIAVFEVALLFESGMHEKMDRNILIACEEEQRIQRIVERDNTTRQAAL
                     ARIRAQMS"
BASE COUNT          459 a          490 c          520 g          343 t
ORIGIN      
        1 tctgcgaacg ctttgacgcg cgagcaaagc ccaacgcttc catgctcttt tccatgcaga
       61 aacgcatgac gcgcgaactg gagcaggccg gcatgacaaa gctctatacc gagatggaac
      121 tgccgctttc gagcgtgctg ttcgatatgg agcgcgtcgg tttccgcgtg gatgaagaca
      181 tcttaaaaga actccaaacg cgattcaacg agcaaagcga ggcgctggct aagcagattt
      241 attcgctcac gggagagacg ttcaatattc tttcacccaa gcagcttggc gaagtgctgt
      301 ttgtcaagct cggtctgccg acagggcgaa agaacaagtc cggcttttca acggatgcgg
      361 acacgctcga aagcctgcgc gaccgccatc ccgcggttcc gatgatcctt gagtaccgct
      421 ttatcacgaa actcaagagc acgttcgtag acggactgct cgccgttcgc agcgaaaaag
      481 acggaagagt gcgcacgtag tttaaccagt gcgtaaccgc aacgggcaga atctccagca
      541 cggagccgaa catgcagaat atccccgtac ggactgcgca ggggcgcgaa atccgcaaag
      601 cgtttgtggc ttccaaaggt aatgtgctcg tggacgcgga ttattcgcag atcgagctaa
      661 ggctgcttgc gcatatcagc ggggacgagg gtatgattgc cgcgttcaac ggcggaaagg
      721 acatccatcg cacaaccgcg agcgaagtgt tcggcgtgcc gtttgacgaa gtgaccagcg
      781 acctgcgcaa cgccgcaaaa gcggttaact tcggaatcgt gtacggcatc agcgacttcg
      841 gtttggcgcg gaacctcgat attcccgtca agcgcgcggc ggaatacatc aagctctatt
      901 ttgagcggta cccgcgcgtt aaagcctatc tggacgagag cgtggagtcc gccaagcgca
      961 ccggatatgc cgtaaccatg ttcgaccgca ggcgcgccat gacggaactc aaaagctcca
     1021 actacaacac gcgctcattt ggcgagcggg tcgctatgaa catgcccatc cagggcacgg
     1081 cggcggacat catcaagctc gcaatggtta aggttcaccg gattctcaac gagcgcggac
     1141 tgaaaaccaa actgattctg caggtgcacg atgaactgat ctttgacacg ccgccggacg
     1201 agcgggacga ggtcgttctg ctcgttcacg actgcatgga gcaggtggct cagctcagcg
     1261 tcccgctcga cgcggacgtg aaagttggaa agagctggtt cgaaacgaaa tgagcaagcg
     1321 cgcttacaaa cgtatcgggc tgaccggcgg catcggcgcg ggcaaatcga ccgctgcaaa
     1381 acgcttccgc gaacttggcg tgctggtgtt ggacgcggat gaaatttcgc gcgcctcgct
     1441 gaaaaaaggc ggcgcgtgct atgacggcgt cgttgcattg tttggctctg aaattctagc
     1501 gcaggatggc gaaatcgacc gcaaaaaact tgccggaatc gtttttgctg atgaagaaaa
     1561 acgccgacaa ctcaacgcca tcgtgcatcc gtatgttata gaaacaatgt tcagcagagc
     1621 cgaagaaacg ctaaacaaaa gcggcggaat cgccgttttt gaagtagcgc tgctgtttga
     1681 aagcggcatg cacgaaaaaa tggaccgcaa tattctcatt gcctgcgagg aagaacagcg
     1741 catacagaga attgttgaaa gggacaacac cacgcgccaa gccgcgctcg cccgcatccg
     1801 cgcacaaatg tc
//
LOCUS       DAOMGY010000382         1806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_75636_length_1806_cov_4.034266, whole genome shotgun sequence.
ACCESSION   DAOMGY010000382 DAOMGY010000000
VERSION     DAOMGY010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75636_length_1806_cov_4.034266"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLR57_06310"
     CDS             <1..203
                     /locus_tag="PLR57_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HQQ41106.1"
                     /translation="EKAKAFISEIRRMNRDMKIQDTFDFIQDKDIPQMIKWAMKEGNP
                     IYPVPVLWHEPEFEKTIARIRA"
     gene            219..947
                     /locus_tag="PLR57_06315"
     CDS             219..947
                     /locus_tag="PLR57_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HQQ41107.1"
                     /translation="MENERYARQILMPQIGEQGQKKLSRSSALVVGCGGLGSTLLLNL
                     CGMGVGRIGFCDGDVVSRSNLNRQFLHTPRDIGRQKAQSAYEKLSAFAPELTLEPYAF
                     YLTKENAQDIVGLYDVVVLAVDSLPARLVVNEACVKQRVTLIDGGINGLHGTLLTVRP
                     GKTACLHCLYGDVPPPLGAVASFAPVASMVSAMQAQAAANILLGLDNPSDGTMILLDG
                     AQLTTERVRVRRREDCPVCSKLHN"
     gene            complement(1001..1594)
                     /locus_tag="PLR57_06320"
     CDS             complement(1001..1594)
                     /locus_tag="PLR57_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQQ41108.1"
                     /translation="MTDAQGKKLYSVPETYRIIVNAMRSIPGMKRARRSGEMTTPFME
                     RIMLAVTEVNGCALCSYYHTRVSLENGMSAEEIKRMLGGEFADAPEAELPAILFAQHY
                     ADTRGMPSKEAWERILDVYGETGAYGVLAATRMIMMGNAFGIVWGSFFNRFRGKADPR
                     SSLGYEIAQLICTIPLVLAGMIHAGILRLMKKPVLTF"
     gene            complement(1602..>1806)
                     /locus_tag="PLR57_06325"
     CDS             complement(1602..>1806)
                     /locus_tag="PLR57_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236910.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HQQ41109.1"
                     /translation="YREKLTSCGMVLSGTSPDGRIIETIELKDHPFYIGVQFHPEFKS
                     RPNRAHPLFKGFIAASLKNKAQD"
BASE COUNT          374 a          546 c          512 g          374 t
ORIGIN      
        1 aggagaaagc caaagccttt atttcggaaa tccgccgcat gaaccgggat atgaagattc
       61 aggatacgtt cgactttatt caggacaagg atatcccgca gatgatcaag tgggccatga
      121 aggaaggtaa ccccatctac cccgtccccg tgctctggca cgagccggaa tttgaaaaaa
      181 cgattgcgag aatccgcgca tagcgtatac tggagagtat ggaaaacgaa cggtacgcgc
      241 gacaaatcct catgccgcaa atcggcgaac aggggcagaa aaaactgtcc cgctcttccg
      301 cgctggtcgt aggctgcggc gggctcggtt ccacgctgct gctgaacctc tgcggcatgg
      361 gcgttggcag aatcggcttc tgcgacgggg acgtcgtctc gcgttccaat ctcaaccgtc
      421 agtttttgca caccccgcgc gatatcggcc gacagaaagc gcaatccgct tacgaaaagc
      481 tatccgcgtt tgcgccggag ctcacgttgg agccttacgc cttttatctg acaaaggaga
      541 acgcgcagga tatcgtcggc ttgtacgacg tggtcgtcct cgcggtggat tccctgcccg
      601 cgcggctggt cgtcaacgaa gcttgcgtga aacagcgcgt aacgctcatc gacggcggca
      661 tcaacggcct gcacggaacg ctcctgaccg tccgccccgg aaagaccgcc tgtctgcact
      721 gcctctacgg ggacgtcccg ccgccgctcg gcgcggtagc gagctttgcc ccagtagcct
      781 ctatggtttc cgccatgcag gcgcaggccg ccgccaacat cctgctggga ctggataacc
      841 cgtccgacgg gacgatgatc ctgctcgacg gcgcgcagtt aaccacagag cgcgtccgcg
      901 ttcgccggcg ggaagattgc ccggtctgca gtaagctcca caactaaaat agtacaacgc
      961 aaagcggcgg ggtttccccg ccgcttttgt attatacaaa ctagaatgtc agcaccggct
     1021 ttttcatgag ccgcagaatc cccgcgtgaa tcatgcccgc aagcacgagc ggaatcgtgc
     1081 agataagctg cgcaatttcg tatcccaggc tgctgcgcgg gtcggctttg ccgcgaaaac
     1141 ggttgaaaaa cgaaccccat acgatgccga acgcgttgcc catcatgatc atgcgcgtcg
     1201 ccgcaagaac gccgtacgcg cctgtctcgc cgtatacgtc gagtatgcgc tcccatgctt
     1261 ctttggaagg catgccgcgc gtatcggcat aatgctgggc aaagagaatc gcgggcagtt
     1321 ccgcttccgg cgcgtcggca aactcaccgc cgagcatgcg cttaatctcc tcggcgctca
     1381 tgccgttttc cagcgaaacg cgcgtgtgat aatacgagca gagcgcgcag ccgttcactt
     1441 ccgttaccgc cagcatgatt cgctccataa aaggcgtggt catttcgccg cttctgcgcg
     1501 cgcgcttcat gcccggtatc gagcgcattg cgttgacgat gatacggtag gtctccggta
     1561 cggaatagag ttttttaccc tgcgcgtctg tcatcttttc cttagtcctg cgccttgttc
     1621 ttgagcgacg ccgcgataaa tcccttgaac agcgggtgcg cacggttcgg gcggctcttg
     1681 aattccgggt ggaactgcac gccgatgtag aacggatgat ccttcagctc tattgtctcg
     1741 atgattctgc cgtccggcga cgtgcccgaa agcaccatgc cgcaggaggt cagcttttcg
     1801 cggtag
//
LOCUS       DAOMGY010000383         1804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_75793_length_1804_cov_2.982276, whole genome shotgun sequence.
ACCESSION   DAOMGY010000383 DAOMGY010000000
VERSION     DAOMGY010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75793_length_1804_cov_2.982276"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..166
                     /locus_tag="PLR57_06330"
     tRNA            91..166
                     /locus_tag="PLR57_06330"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Gly,seq:gcc)
     gene            236..312
                     /locus_tag="PLR57_06335"
     tRNA            236..312
                     /locus_tag="PLR57_06335"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:270..272,aa:Lys,seq:ctt)
     gene            423..1145
                     /gene="yqeC"
                     /locus_tag="PLR57_06340"
     CDS             423..1145
                     /gene="yqeC"
                     /locus_tag="PLR57_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HQQ41110.1"
                     /translation="MQTIFHGKNDSLAGAFGVPKGVTSMVGGGGKTTLMLALARELAA
                     AGQRVIVSTTTHIFPPESITTLTNTTADDVRFVLQSEPLICLGTPAEHGKLSAPKLTV
                     SEMARLADYVLLEADGAKRLPLKAPAEHEPVIPPETTLVIAVLGLDGIGKPVSEAVFR
                     PERYAALIGKPESEPVTPLDAALVLTHPDGQRKNVTGTMRFCVLLNKADDEKRSAFAL
                     ELARELNGTGLAERTVISALGK"
     gene            1192..>1804
                     /gene="yqeB"
                     /locus_tag="PLR57_06345"
     CDS             1192..>1804
                     /gene="yqeB"
                     /locus_tag="PLR57_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HQQ41111.1"
                     /translation="MLIWIRGAGDIASGVAFRLWHSGFSVVMTDLPQPTSIRRTICFS
                     EAIINGTARVEDITGRHAKNAEEAREILSAGEIAVLADPDGKEAKKLALDAVVDAILA
                     KRNIGTSMKDAEIVVGVGPGFTAGMDCHAVVETMRGHTLGRAYYAGSALPNTGVPGNI
                     GGFTLERILRAPRAGVFHGVKAIGDTVSKGEICAYVDGEPIVSR"
BASE COUNT          388 a          524 c          553 g          339 t
ORIGIN      
        1 gagcacctga ctcttaatca gggtgtccag ggttcgaatc cctgaggggg taccaatttt
       61 cccgccgcag tcggtatgcg gcaaactttt gcgggagtag ctcatttggt agagcgcagc
      121 cttgccaagg ctgaggtggc gggttcgagc cccgtctccc gctccattta ttaggaggcg
      181 tttacgcccc ctaataaatg gaccggtaat cagaggggct cgaagccccg tacatgcctc
      241 cttagctcag ttggctagag cacctgactc ttaatcaggg tgtccagggt tcgaatccct
      301 gagggggtac cagaaagtcc gcacagcaat atgtgcgggc tttttctctc tattgttatg
      361 cgaccagtat ttgacaattc ctccggcaaa gtgctattct cagacgggga gaacgagcgc
      421 ttatgcaaac tatcttccac ggtaaaaacg attcgcttgc cggcgcgttc ggcgtgccga
      481 aaggcgtaac cagtatggtt ggcggcggcg gtaaaacgac gctgatgctt gcgcttgccc
      541 gcgaactggc ggcggcgggg caaagggtca tcgtttccac cacgacgcat atctttccgc
      601 cggaaagcat cacgactctc acaaacacca cggcagacga cgttcgcttc gttcttcaaa
      661 gcgaaccgct catctgcctc ggcacacccg ccgaacacgg aaaactctcc gcgccgaaac
      721 tgaccgtcag cgaaatggcg cgtcttgcgg attacgtcct gctggaagcc gacggcgcaa
      781 agcggctgcc gctcaaagcg cccgccgagc acgaaccggt cattccgccg gaaacgacgc
      841 tcgttatcgc cgtgctgggg ctggacggaa tcggcaaacc cgtcagcgaa gccgtgttcc
      901 ggccggaacg gtacgccgcg ctgattggca agccggagtc ggaacccgta accccgctcg
      961 acgccgcgct ggtgttgaca catccggacg gtcagcgaaa gaacgtaact ggtaccatgc
     1021 gcttttgtgt tctgctcaac aaggcggacg acgaaaagcg aagcgctttt gcgcttgagc
     1081 tggcacgcga gttgaacggc acggggctgg ctgaacgaac cgtaatctcc gcattgggaa
     1141 aataaaagcg aaacaccttt ctatcgcgaa cgaaagcgag gaatataaac tatgttaatt
     1201 tggattcgcg gggcggggga tatcgcctcc ggcgtcgcct tccggctttg gcacagcggg
     1261 ttttcggtcg tgatgaccga cctgccgcag ccgacctcca tccgccggac gatctgcttt
     1321 tcggaagcga tcatcaacgg cacggcgcgc gtggaggaca ttacaggccg tcacgccaaa
     1381 aacgccgaag aagcgcgtga aattctcagc gcgggcgaaa tcgctgtgct ggcggacccg
     1441 gacggtaagg aagcaaagaa gctcgcgctg gacgcggttg tggacgcgat tctcgccaag
     1501 cgcaacattg gaacatccat gaaggacgcg gagatcgtcg tcggcgtcgg ccccggcttc
     1561 acagcaggaa tggactgcca cgcggtggtc gagaccatgc gcggccatac gctcggcaga
     1621 gcgtactacg cgggttccgc gctcccgaat acgggcgtgc cgggcaatat cggcgggttc
     1681 acgctggagc gcattctgcg cgcgccgcgc gcgggcgtat tccacggcgt aaaagcaatc
     1741 ggggacacgg tgagcaaagg cgaaatctgc gcgtatgtgg acggcgagcc gatcgtttcg
     1801 cgca
//
LOCUS       DAOMGY010000384         1802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_75917_length_1802_cov_3.681168, whole genome shotgun sequence.
ACCESSION   DAOMGY010000384 DAOMGY010000000
VERSION     DAOMGY010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75917_length_1802_cov_3.681168"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLR57_06350"
     CDS             complement(<1..238)
                     /locus_tag="PLR57_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HQQ41112.1"
                     /translation="MRYSEMDKQALAKELERARADYETLRGHKLNLNLTRGKPETAQL
                     ALSDGMFATVLDGNFVSDGIDARNYGELAGLPACR"
     gene            complement(479..961)
                     /locus_tag="PLR57_06355"
     CDS             complement(479..961)
                     /locus_tag="PLR57_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQQ41113.1"
                     /translation="MQKQLVKCNINGKDVETYVDVRASLTDMLRNDYRLTSVKKGCEV
                     GECGACNVIIDGECYNSCIYLAVWAEGKHIRTLESLLGPNGELSDIQQAFVDEAAVQC
                     GFCTPGFIMTAVEILETGKEYTRDELRKLLSGHLCRCTGYENILNAVEKTMKKRLGKA
                     "
     gene            complement(961..>1802)
                     /gene="xdhB"
                     /locus_tag="PLR57_06360"
     CDS             complement(961..>1802)
                     /gene="xdhB"
                     /locus_tag="PLR57_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HQQ41114.1"
                     /translation="NAVELMQAHPSAVLIAGGSDVLIKMREGKLAGAELISLYKLDEL
                     REVTIDSDGALRIGPMTSFSHATKSPVLQKYMPVLGEAADTVGGPQLRNIGTLGGNIC
                     NGVTSADTASTQVAYDADLEIASVNGTRRMSIHDFYKGPGKVALEPGELLTAILIPKE
                     SYENCFGYYIKYAMRNAMDIATIGCSVNVVLSADKKTIQRARIAYGVAGPVPMRAKTA
                     EAALAGQPVGEAAIEIVGKEVQNDVNPRTSWRASADFRRQIISETARRGLKAAIERAG
                     GVF"
BASE COUNT          329 a          497 c          535 g          441 t
ORIGIN      
        1 tgcggcaggc ggggagaccc gcaagctcgc cgtagttgcg cgcgtcgata ccgtccgaaa
       61 cgaagttgcc gtccaatacg gtagcgaaca ttccgtccga gagggcgagc tgtgcggttt
      121 cgggcttgcc gcgggtcagg ttgaggttga gtttgtgtcc gcgcagggtt tcgtaatccg
      181 cgcgggcgcg ttcgagttct ttagcgaggg cttgcttgtc catttccgaa taacgcatgt
      241 ctagaggtcc tttccgctta tattaaaaaa aggcccgaaa ccggtttgtt tgcggcttcg
      301 ggcggcgaaa cgaaacgatt attccatatg ctacgatatt ttggagcgga tttcaatcac
      361 taattttata tctattcata taaatatgaa agaaacgcgc ataacgtgca tatttggaat
      421 gcttttatct gcgattacgc agggattgcg cggtttaagg ggaaaacgaa tactgtggtt
      481 atgctttccc cagacgtttc ttcatggtct tttcaacggc gttgaggata ttttcatacc
      541 ccgtgcagcg gcagaggtgg ccggagagga gctttctgag ctcgtcgcgc gtgtattcct
      601 tgccggtttc cagaatctcc acggcggtca taataaatcc cggcgtgcag aaaccgcact
      661 gaacggcagc ttcgtccaca aacgcctgct ggatatcgct gagctcgccg ttggggccga
      721 gcaggctctc cagcgtgcgg atgtgcttgc cttccgccca gacggcgagg tagatgcagg
      781 agttgtagca ttccccgtcg ataatgacgt tgcaggcgcc gcattcgccg acttcacagc
      841 ctttcttcac ggaagtcagg cggtagtcgt ttcggagcat atccgtcagc gacgcgcgga
      901 cgtccacata cgtttccacg tccttgccat ttatgttgca ctttacaagc tgtttctgca
      961 ttagaacaca cctcctgcgc gctcaatggc ggctttcagg ccgcgccgcg cggtctcgct
     1021 gatgatctgc ctgcggaagt ccgccgaggc gcgccagctc gtgcgcgggt taacgtcgtt
     1081 ttgcacttct tttccgacaa tctcaattgc cgcttcgccg acgggctgac ccgcaagcgc
     1141 ggcttccgcg gttttggcgc gcatgggcac aggccccgcg acgccgtacg cgatgcgcgc
     1201 gcgctggatc gtctttttat ccgcactcag cacaacgttg accgagcagc cgatggtcgc
     1261 gatgtccatg gcgttgcgca tggcgtattt gatgtagtag ccgaagcagt tttcgtagct
     1321 ctctttgggg attaaaattg ccgtgagcag ttcgccgggt tcgagggcga ccttgccggg
     1381 gcctttgtag aaatcgtgaa tgctcatgcg ccgcgtgccg tttacactcg caatttccag
     1441 atccgcgtcg tatgccacct gcgtggaggc agtgtccgcg ctggttacgc cgttgcagat
     1501 gttgccgcca agggtgccga tgttcctgag ctgcgggccg ccgacggtat ccgcggcttc
     1561 gccgagcacg ggcatgtatt tttgcagcac ggggctcttg gtcgcgtggg aaaaactcgt
     1621 catggggcca atgcgcagcg cgccgtcgga atcgattgtg acttcgcgca gttcgtcgag
     1681 cttatagagg ctgatgagct cagcgcccgc gagcttgcct tcgcgcattt taatcaaaac
     1741 gtcgctgccg cctgcaatca ggacggccga cggatgcgcc tgcatgagtt ccacggcgtt
     1801 ct
//
LOCUS       DAOMGY010000385         1800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_76065_length_1800_cov_3.257307, whole genome shotgun sequence.
ACCESSION   DAOMGY010000385 DAOMGY010000000
VERSION     DAOMGY010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76065_length_1800_cov_3.257307"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..759)
                     /locus_tag="PLR57_06365"
     CDS             complement(64..759)
                     /locus_tag="PLR57_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HQQ41115.1"
                     /translation="MRKPENNISIHESFSVGALLALAGGFLDAYTYLSRGGVFANAQT
                     GNMVLLGIRLAERNWGVAVRYLIPIIAFAAGVLLAEWIQSALKGRANVRWRQAVLLLE
                     IALLAIIGFVPDGRWNWAVNVAVSFVCALQVEAFRTMHGLSYATTMCTGNLRSGTECI
                     FLYFQTKNEVLLSNGIKYYAIIVVFIVGAMLGVFLTGWMAERAVWVPAGLLVIVFSLL
                     FQCAAEQSVPPLP"
     gene            complement(818..1723)
                     /locus_tag="PLR57_06370"
     CDS             complement(818..1723)
                     /locus_tag="PLR57_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41116.1"
                     /translation="MDAVVQWLLEGDVSIHYLTHRYLLESDADVLLRLQNRIENEGYG
                     AAYLALQNENGHWGIWYYQPKWTSTHYTLLELKNIGLPAANAPCREITSRMLSECMLP
                     DGGLNLAKSEHASDSCVDGMALSYAAYFLAGDARLKRLVDHLLDSQRPDGGFTWNPNS
                     NFSDPHSTICVLEGFSEYLACGASHRKDQIAAAQANAVSFLLENKLFFSGDQRYRMLA
                     YPFRYRYDLLRALEYLALEHMPFCEPMRPALDWLKSKQSREGFWNLELEHRGAVHFPL
                     ETRGSPSRFITLKALVILKHFQSIS"
BASE COUNT          410 a          508 c          508 g          374 t
ORIGIN      
        1 gatcatcacg ttgccgagct tgattccggg tatcattatg ttgcctcctt catttggtta
       61 atatcatggc agtggtggaa cagactgctc cgccgcgcac tgaaacagca gcgaaaaaac
      121 gatgaccagc agacccgccg gaacccagac cgcgcgctct gccatccagc cggtgagaaa
      181 cacgccgagc atcgcgccca cgatgaaaac gacgataatc gcgtaatact ttatgccgtt
      241 tgagagcagt acttcgtttt ttgtctggaa gtagagaaaa atgcactccg tgccgctgcg
      301 gaggttcccg gtgcacatgg tggttgcgta cgaaagcccg tgcatggtgc ggaacgcttc
      361 cacctgcagc gcacagacga acgataccgc aacgttgacg gcccagttcc aacggccgtc
      421 cggcacaaag ccgatgatgg ctaacagcgc gatttccagc aacagcacgg cttgccgcca
      481 gcggacgttg gcgcggcctt tcagcgcgga ctgaatccac tcggcaagca gaacgcccgc
      541 cgcgaatgcg atgattggga tgaggtagcg caccgcgacg ccccagttcc gctctgcaag
      601 gcgaatgcca agcagcacca tgttgcccgt ctgcgcgttg gcgaataccc cgccgcgaga
      661 aaggtaggta tacgcgtcca gaaacccgcc agcgagcgca agcagcgcgc cgacggagaa
      721 ggattcatgt atggaaatat tgttttcggg cttccgcatc ggttgctgtt ccttaagttg
      781 gaaatctgat acgaagaaca gggaaggggc actgttatca ggagattgac tgaaaatgct
      841 tgagaatgac aagcgctttc agcgtgataa agcggctcgg tgaaccgcgc gtttccagag
      901 ggaagtgtac cgcgcccctg tgctccagct caagattcca gaatccttcg cgggactgct
      961 tgctcttgag ccagtcgagc gcagggcgca tcggttcgca gaacggcatg tgctccagtg
     1021 cgagatactc cagcgcgcgc agaagatcgt accggtaacg gaagggataa gcgagcatcc
     1081 ggtagcgctg gtcgccggaa aagaataact tgttctccag caaaaacgat accgcgtttg
     1141 cctgcgccgc ggcaatttgg tccttgcggt gggatgcgcc gcaagcaagg tattccgaaa
     1201 acccttccag cacgcagatc gtcgagtgcg gatcgctaaa gttcgaattc ggattccagg
     1261 taaagccgcc gtccggccgc tgagagtcga gcagatgatc gacaagacgc ttcagccttg
     1321 cgtcgcccgc aagaaaatac gcggcgtaac tgagcgccat gccgtccacg cagctgtcgc
     1381 ttgcgtgttc gctcttagca aggttcagtc cgccgtccgg cagcatgcac tcgctcagca
     1441 tccggctcgt gatctcgcgg catggcgcat tagcggcagg aagtccgata tttttcagtt
     1501 caagtagcgt gtagtgcgtg gacgtccatt tcggctgata gtaccagatt ccccagtggc
     1561 cgttttcgtt ctggagagcg agataagccg cgccataccc ttcattttcg atccggtttt
     1621 gcagtcgcag caaaacatcc gcatccgact ccagcaggta acgatgcgtc agataatgta
     1681 tggaaacatc gccttccagc agccattgaa ccacggcgtc catcgtgttg ctccctcgcg
     1741 tttatttgag tctattatgg gtcaatcatt attctcgtgg gattgaccat gccacactat
//
LOCUS       DAOMGY010000386         1800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_76087_length_1800_cov_2.283095, whole genome shotgun sequence.
ACCESSION   DAOMGY010000386 DAOMGY010000000
VERSION     DAOMGY010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76087_length_1800_cov_2.283095"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1011
                     /locus_tag="PLR57_06375"
     CDS             49..1011
                     /locus_tag="PLR57_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HQQ41117.1"
                     /translation="MASPEELRNRIKLAGIVGQGGAAFPTFVKLSPPATKKIEYLIIN
                     GCECEPYLTRDYRLMLERPADIIAGLKISMKASGAETGIIGIEENKPEAIRILSELVK
                     NENGIFVESVKTKYPQGSEKQIIHVVTGREVPRGKLPVDAGVLVFNVSTAAAIADAVL
                     LGKPLVERITTVTGCVKTPSNLRLRVGTTYVQAFEACGGLCDDVGKIFAGGPMTGLCA
                     ADTSVSMTKATNGIVAFSQQQAKQIEETACIRCARCAQVCPIGLHPYQMRADIDRPDL
                     ELAKKNGLLDCVLCGACSYICPARRYLASSFKAAREELAAKARR"
     gene            1017..>1800
                     /locus_tag="PLR57_06380"
     CDS             1017..>1800
                     /locus_tag="PLR57_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HQQ41118.1"
                     /translation="MEQTKRLTVSGAPHIFSKNTTTVLMLDVIVALVPVIAVGIWLFG
                     WNAARVLIISASSCVIFELLWELLLKKPITAGDLSAVVTGVLLGLNLPSTAPWWMIVI
                     GSAVAILLVKQLFGGLGDNFVNPALTARAVLLASWPAFMSGSSAFVTPFDAVSSATPI
                     DIISSATVHSTAYPSNLDLFIGWVPGCIGEVSKVAILLGLLYLLIRKTISWHIPLTMI
                     GSFMLCIFLFGGDPVQAVLTGSILFGAVFMATDYVTSPMTHTG"
BASE COUNT          407 a          481 c          504 g          408 t
ORIGIN      
        1 aaatcggata acggtgaaat tcgtaaattt gagcctataa atcccgagat cgcatcgcct
       61 gaggaacttc gcaacagaat taaacttgcc ggaattgtag gacagggagg tgcagctttt
      121 cccacttttg taaaattgag tccgcctgca acaaaaaaaa ttgaatactt aattattaac
      181 ggctgcgagt gcgagcctta tttaacccgc gattacagac ttatgcttga aagaccggct
      241 gatattattg ccggtttgaa aatatcgatg aaagcttccg gtgcagaaac cgggattatt
      301 ggaattgaag aaaacaaacc ggaagcaata agaattctaa gcgaattagt taaaaatgaa
      361 aatggaatat ttgttgagtc agtaaaaaca aaatatccgc agggctcgga aaagcagatt
      421 atccacgttg taaccgggcg cgaagtgccg cgcggcaaac tgcccgtcga cgcgggcgtg
      481 ctggtgttca acgtcagcac cgcggcggcc attgcggacg ccgtcctgct gggaaaaccg
      541 ctcgtcgaac ggattacgac cgtgaccggc tgcgtcaaaa cgccatccaa cctcaggctc
      601 cgcgtgggaa cgacctacgt tcaggcgttc gaagcctgcg gcgggctctg cgacgacgtt
      661 ggcaaaatat ttgctggtgg gcctatgacg ggtctatgcg cggcagacac gagtgtttcc
      721 atgaccaaag cgaccaacgg aatcgttgcg ttcagccaac agcaggcaaa gcagattgaa
      781 gaaaccgcct gcatccgctg cgccagatgt gcgcaggtct gtccgatcgg actgcatccc
      841 tatcagatgc gcgcggatat cgaccgtccg gatttggaac tggcaaagaa aaacgggctg
      901 ctcgactgcg tgctctgcgg cgcatgctcg tatatttgcc ccgcgcgcag ataccttgcc
      961 tcgtcgttta aggcggcgcg ggaagagctt gccgcaaagg cgaggaggtg aggagtatgg
     1021 aacagacaaa acgattaacc gtttccggcg cgccgcatat cttctcaaag aatacgacaa
     1081 ccgtgctgat gctggatgtt attgtggcgc tggtgcccgt aatcgccgtc ggtatctggc
     1141 tgttcggctg gaacgccgcg cgcgttctga ttatcagcgc ttcgagctgc gtaatctttg
     1201 aactgctctg ggaactgctg cttaaaaaac ccattaccgc aggcgacctt tctgcggtcg
     1261 tcaccggcgt gctgctgggg ctgaacctgc cctccacggc accctggtgg atgatcgtca
     1321 tcggcagcgc ggttgctatc ctgctggtca aacagctctt tggcggcctt ggcgataact
     1381 tcgtcaaccc ggcgctcacg gcgcgcgcgg tgctgctggc tagctggccc gcgttcatga
     1441 gcggcagcag cgcgtttgta accccgtttg acgcggtttc cagcgcgacg cccatcgaca
     1501 ttatctccag cgcgacggtg cattccacgg cgtatccttc gaatctcgac ttattcatcg
     1561 gctgggttcc cggctgcatc ggcgaagtca gcaaagtcgc catccttctg gggcttttgt
     1621 acctgctgat tcgaaagacg atctcctggc atattccgct tacgatgatc ggttcgttta
     1681 tgctgtgcat tttcctcttt ggcggagacc ccgtacaggc cgttctgaca gggagcatcc
     1741 tctttggcgc ggtgttcatg gcgaccgact atgtgacgag ccccatgacg catacgggtc
//
LOCUS       DAOMGY010000387         1796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_76290_length_1796_cov_4.362435, whole genome shotgun sequence.
ACCESSION   DAOMGY010000387 DAOMGY010000000
VERSION     DAOMGY010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76290_length_1796_cov_4.362435"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..876
                     /locus_tag="PLR57_06385"
     CDS             148..876
                     /locus_tag="PLR57_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41119.1"
                     /translation="MNGLQRNTARLFQNGGKLFVLAMDHAQTGIMEGLTDVKDTMRLC
                     ADTELDGFLVNVEPARAMVEPALIQKKLILRSSFGGTRLSSSFSSIHRNHVSPETALS
                     LGADAVVMMIVFGEDDAEGVQQAAADIDSYHQYGIPVIAEILAVDFSKTQTFEVQANG
                     ARAAAELGADVIKAFYTERFDEVVKNCPVPVILAGGPKERDISDVAKAAVADGVSGFA
                     FGRNLFQAENPPELISRLNQVLRG"
     gene            998..>1796
                     /locus_tag="PLR57_06390"
     CDS             998..>1796
                     /locus_tag="PLR57_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HQQ41120.1"
                     /translation="MTKKPELTLLYSIARSYYVDKLSQSQIADMENISRSQISRLLDR
                     AEKLGIVSISVSLPEDPDLSEMAESVTKLLGLREVIVAPLEGGADDSAAIAEAISVAA
                     SQYLPRALKGTKTVGLGWGKSMYNMSLRLSYRNLGDERMYVPLIGMSGTSNPYLQINT
                     IVDRVAERHRARSYFVSVPAFRELSSPFSDIEEQRMAMLEQYWSHLDAAVIGLGGPPQ
                     SGGLFVDEISERYTAELAASGAVGDILSYFFRPDGGVYHFGHAYRQVA"
BASE COUNT          370 a          512 c          511 g          403 t
ORIGIN      
        1 ggcggctttg caacaaatac atgcacattg ttgcatctat agagcgttgc gttgacactt
       61 cttctatcca gttaatataa taatgatggt tagaaatacc attgtttcca ttaactccta
      121 tcataaatta aaggcggttt ccagtacatg aacggtttac agcgcaatac ggcaaggctt
      181 tttcaaaacg gcggcaagct ctttgttctg gcaatggatc acgcgcagac gggcattatg
      241 gaaggcttaa cggacgttaa agacaccatg cgactctgtg cggataccga gctggacgga
      301 ttcctcgtga atgtggagcc tgcgcgcgcc atggtcgaac ccgcgcttat tcaaaaaaag
      361 ctgattctcc gttcgtcctt tggcggcacg cgcctttcga gctcgttttc cagcattcac
      421 cggaaccatg tttctcccga aacggcgctg tctctcggcg cggacgcggt ggtcatgatg
      481 atcgtattcg gcgaagacga cgcagagggc gtccagcagg cggcggcgga tatcgactct
      541 taccaccaat acggcattcc ggtcatcgcg gagattctcg cggtcgattt ttccaagacg
      601 cagacgttcg aagtgcaggc aaacggcgcg cgcgctgccg ccgagctcgg cgcggacgtg
      661 attaaagcgt tctataccga acgcttcgac gaagtggtca aaaactgccc ggtgccggtc
      721 atcctcgcgg gcgggccgaa agagcgggac atctcggatg ttgccaaagc cgccgtcgcc
      781 gatggggtca gcggctttgc gttcggaagg aatctcttcc aggcggaaaa cccgccggag
      841 ctgatttcgc gcttaaacca agtccttcgc ggttaagtcc gcgcttctag ttatttttgc
      901 gtttcatttt tgcgttgcgg ccgcgccgca aacattttct gccgggacgg gcggttgatt
      961 ttcatagagc ctcattttta acataattgg agcgaatatg acaaagaaac cggagctgac
     1021 gctgttatac agcattgcgc gcagctacta tgtcgacaag ctgtctcaga gccaaatcgc
     1081 ggatatggag aacatttctc gttcccagat ttcgcggctg ctcgaccgcg cggaaaaact
     1141 gggtattgta tccatcagtg tatccctgcc ggaggatccc gatctcagcg aaatggccga
     1201 atcggtcacg aagctgctcg ggcttcgcga ggtcatcgtc gccccgctgg aaggcggcgc
     1261 ggacgattcc gccgctattg cggaggccat ttcggttgcc gcgtcgcagt acctgccgcg
     1321 cgcgctcaag ggaacaaaaa ccgtcggcct cggctggggc aagtcgatgt acaacatgtc
     1381 gcttcgcctc tcctaccgca accttggcga cgaacggatg tatgtgccgc tcatcggcat
     1441 gtccggcacg tccaacccgt atctgcagat caacacgatc gtcgaccgcg ttgccgagcg
     1501 tcaccgggcg cggagctatt ttgtgagcgt tccggcgttt cgcgagctat ccagcccgtt
     1561 cagcgatatc gaggaacagc gcatggccat gctcgagcag tactggtcgc atctcgacgc
     1621 ggctgtcatc ggcctcggcg gcccgcccca gagcggcggt ttgttcgtgg acgaaatcag
     1681 cgaacgatac accgcggaac tcgccgcttc cggagcggtc ggagacatcc tctcctactt
     1741 cttccgtccg gacgggggcg tttaccactt cgggcacgct taccggcagg tcgcgt
//
LOCUS       DAOMGY010000388         1796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_76312_length_1796_cov_2.890293, whole genome shotgun sequence.
ACCESSION   DAOMGY010000388 DAOMGY010000000
VERSION     DAOMGY010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76312_length_1796_cov_2.890293"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PLR57_06395"
     CDS             complement(<1..868)
                     /locus_tag="PLR57_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQQ41121.1"
                     /translation="MNNCGISRWDDPREINARLKKLTSEPIYEITSDYYEGEVLRYFD
                     EQCKKSRAVYEEAKEYIPGGVQHNLAFNKPFPICVSKADGAYLTDADGNRYIDFLQAG
                     GPTILGSNNETIRKAVLELINECGPVTGLLHEAELLIAKEIHKHMPAVEMFRMLGSGT
                     EAVMASLRIARIATGNKRIIKIGGAYHGWSDQMVYGLKIPGSRALLESHGIPSACYSL
                     TDEVRPNDLNMLEAMLRRNRLLGGTAAVIVEPVGPESGTRPVAKEYNAGVRALCDQYR
                     ALLIFDEVVTGFR"
     gene            complement(1002..1577)
                     /locus_tag="PLR57_06400"
     CDS             complement(1002..1577)
                     /locus_tag="PLR57_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQQ41122.1"
                     /translation="MSEKKRTKTQRAILHAAKGLYEKHGVGSVPFDDIAEAAGVCRTT
                     VFNHFADAGELQAALAVAEIGDLIDFCSRSDKRGLELIEALLDQLISDTVNYPRVMAR
                     LTNATILGGDAGRVAEIERIIARHYEVEFGKFPESAPVSAALLAEMTMGMYYGQVNHL
                     LAHGLPFDAESMRRDMRAMLGCMLRCVQPKE"
     gene            complement(1574..>1796)
                     /locus_tag="PLR57_06405"
     CDS             complement(1574..>1796)
                     /locus_tag="PLR57_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41123.1"
                     /translation="YAVFSPLSTLWGPALTNIGWNEYIVLVGTMLINLTTEFLYWRLV
                     IYRDSINTNARAKRQQAELEETESEEAAK"
BASE COUNT          317 a          543 c          523 g          413 t
ORIGIN      
        1 cgcggaaacc ggttaccacc tcgtcgaaga tcagcagcgc gcggtactgg tcgcagagcg
       61 cgcgaacgcc cgcgttgtac tcttttgcca cagggcgtgt gccgctttcc ggccccacgg
      121 gttccacgat gaccgccgcc gtaccgccaa gcaggcggtt tctgcggagc attgcttcca
      181 gcatgttcaa gtcgttgggg cgcacttcgt ccgtcagcga atagcaggcg ctcggaatgc
      241 cgtgggattc caacagcgcg cggctgccgg gaatctttaa gccgtacacc atttgatccg
      301 accagccgtg gtacgcgccg ccgattttga tgatgcgctt gttgccggtt gcgatgcgcg
      361 caatacgcag gctcgccatg acggcttccg tgccgctgcc gagcatgcgg aacatctcca
      421 cggcgggcat gtgcttatgg atttcctttg caatgagcag ttccgcttcg tgcagcaggc
      481 ccgtaaccgg gccgcattcg ttgatgagtt ccagaaccgc cttgcggatg gtttcgttgt
      541 tgctgccgag aatcgtcggg ccgcccgcct gcagaaagtc gatataacgg ttgccgtcag
      601 cgtcggtcag gtaagcgccg tccgctttgg aaacacagat ggggaacggc ttattgaacg
      661 cgaggttgtg ctgcacgccg ccggggatgt actccttggc ttcttcgtat accgcgcggg
      721 actttttgca ctgctcgtca aaatagcgca gcacttcgcc ctcgtaataa tcgctcgtga
      781 tttcgtagat cggttcgctt gtgagttttt tcaggcgcgc gttaatctcg cgcgggtcgt
      841 cccagcggct gataccgcag ttgttcatgg ttttatcctc cagtaattgc atttcattat
      901 taacatgggg ttctcagggg cgtgcccctg agttcagggg tttgggggtg aaacccccaa
      961 gtaattcggt tttgggggtg aaaaaacccc caagaaaacc cctactcttt tggttgaacg
     1021 cagcgaagca tgcagccgag catggcgcgc atatcccgcc gcatgctctc cgcgtcgaac
     1081 ggcaggccgt gcgcaagcag gtggttgacc tggccgtaat acatgcccat tgtcatctca
     1141 gccagcagcg ccgcggaaac cggcgcgctt tccgggaact ttccgaactc cacttcatag
     1201 tgccttgcaa tgatgcgctc gatttcggca accctgcccg cgtccccgcc gaggatggtg
     1261 gcgttggtga gccgcgccat cacgcgcggg tagttcaccg tgtccgaaat cagttggtcg
     1321 agcagcgctt cgatgagctc cagcccgcgc ttgtccgagc gcgaacaaaa atcgatcaga
     1381 tcgccgatct ccgcaaccgc cagcgccgcc tgcagttctc ccgcgtcggc aaaatggttg
     1441 aataccgtcg tgcggcaaac ccccgccgcc tcggcgatgt cgtcaaacgg cacgctgccg
     1501 acgccgtgct tttcgtataa tcccttcgcc gcgtgcagga ttgcgcgctg cgttttggtt
     1561 cgtttcttct cgctcatttc gccgcctcct ccgattcagt ttcctctagt tccgcctgct
     1621 gccgctttgc gcgggcgttg gtattgatgc tgtcgcggta aattaccagc cgccagtaga
     1681 ggaattccgt ggtcaggttt atcagcatcg ttccgactaa aacgatgtac tcgttccagc
     1741 cgatgttcgt cagcgcaggt ccccagaggg tggagagcgg cgaaaacacc gcgtag
//
LOCUS       DAOMGY010000389         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_76361_length_1795_cov_3.667241, whole genome shotgun sequence.
ACCESSION   DAOMGY010000389 DAOMGY010000000
VERSION     DAOMGY010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76361_length_1795_cov_3.667241"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLR57_06410"
     CDS             <1..398
                     /locus_tag="PLR57_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41124.1"
                     /translation="PEELDRMLPHMTPEEIMKHILLYFGSHGDDFVERIAMVITNEKF
                     KNPEAATIYYACIVREPSAYYEALFEKMISRGMIRSLDARMFAEQYNYVSIALTKEYF
                     MAKNGLADLESVVRYMVKTLRFFCSLMQA"
     gene            448..948
                     /locus_tag="PLR57_06415"
     CDS             448..948
                     /locus_tag="PLR57_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41125.1"
                     /translation="MAKRTAPLTLQQKYPPSLPCDCDVCRTFCRRPGWWTVCQARDAM
                     DSGLGARMMLEVSPDRTFGVLSPAFSGCEGNFALQELSHRGCTFLRQGLCELHATSHM
                     PLECRFCHHSSIGAGKACHADLERDWRTARGQALVNLWLNEYGISARFLAAQAALAAS
                     MRGAVR"
     gene            complement(926..1630)
                     /locus_tag="PLR57_06420"
     CDS             complement(926..1630)
                     /locus_tag="PLR57_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004053574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41126.1"
                     /translation="MAQAIAETIFDILYLGFVITVGIIMAIKGRKNQLILKFGLMAAL
                     LGSGDAFHLVPRALALWTTGLEANAASLGIGKLITSITMTVFYLILYYIWRERYQITG
                     KRALTITMWVLAAARVALCLLPQNQWLSYRQPLEFGILRNIPFAVMGIIIIVLFAQEA
                     KKANDKAFRWMWLAVTLSFGFYIPVVLFAEIIPPIGMLMIPKTLAYVWVVLMGWSIFC
                     KELKEEKRGFNAPPRA"
BASE COUNT          397 a          518 c          473 g          407 t
ORIGIN      
        1 cgccggagga gctcgaccgg atgcttccgc atatgacgcc cgaagagatt atgaagcata
       61 ttctcctcta cttcggcagc cacggagacg attttgtcga gcgaatcgcc atggtcatca
      121 cgaacgaaaa gttcaaaaac cccgaagccg ccaccatcta ttatgcgtgc atcgtccgcg
      181 agccttccgc ctattatgaa gcactgtttg aaaagatgat ttcgcgcggc atgattcgct
      241 ccctcgacgc gcgcatgttt gccgaacagt acaactatgt ttccatcgcg ctgacgaagg
      301 aatatttcat ggcgaagaac ggactggccg atctggaatc ggtcgttcgc tatatggtca
      361 aaacgcttcg gttcttctgt tcgctgatgc aggcgtaatc atcagaccgg ctccatgaga
      421 atctgctttg ttcttcggga gtaaattatg gccaaacgaa ccgcaccgtt aacgctccag
      481 cagaaatacc cgccttccct accctgcgac tgcgacgtct gccgcacctt ttgccgcaga
      541 cccggctggt ggacggtttg tcaggcgcgg gatgcgatgg attccgggct cggcgcgcgc
      601 atgatgctgg aagtttcgcc cgaccgaacc ttcggcgtgc tttccccggc gttctccggc
      661 tgcgagggaa atttcgccct gcaggagctg tctcaccggg ggtgtacatt tcttcgacag
      721 gggttatgcg aactgcacgc cacgagccac atgccgctcg aatgccgctt ttgccaccat
      781 tcgtcgatcg gcgcgggaaa agcctgtcac gcggacttgg agcgcgactg gcgcaccgcc
      841 cgcggacagg ctctggttaa tctctggctg aacgaatatg gaatctccgc gcggtttctc
      901 gccgcgcagg cggcgcttgc cgcttctatg cgcggggcgg tgcgttaaac cctctcttct
      961 cttcttttag ctccttgcag aagatggacc agcccatcag cacgacccac acataagcga
     1021 gcgtcttggg aatcatcagc atgccgattg gcggaatgat ctcggcaaag agaacgaccg
     1081 gaatgtagaa cccgaacgat agcgtaaccg caagccacat ccatcggaac gccttgtcgt
     1141 tcgctttttt cgcctcctgc gcaaacagca cgataatgat gattcccatc accgcaaacg
     1201 ggatattcct gaggatgccg aactccagcg gctggcggta gctgagccac tgattctgcg
     1261 gcagcaggca gagcgcaacc cgcgcggccg ccagtaccca catggtaatc gtaagcgcgc
     1321 gcttgccggt aatctgatag cgttcgcgcc agatgtagta gagaatcagg tagaataccg
     1381 tcatcgtaat cgacgtaatc agcttgccga tgcccagcga cgcggcgttt gcttccagcc
     1441 cggtcgtcca gagcgcaagc gcgcgcggta caaggtggaa cgcgtctcct gagcccaata
     1501 atgccgccat gagcccgaac ttgaggatca gctggttttt cctgcctttt atcgccatga
     1561 tgattccaac ggtaatgaca aagcctaaat acagtatgtc gaatatcgtt tccgcaattg
     1621 cctgtgccat gtttttcact cctgcttttt ttgaattctc ccaaaggggg tatagttcat
     1681 ttgttttctg aaaaccctat ccgccgtaaa gaacgcggtt gacaatctga attaaaaacg
     1741 cgtgttcgca ggtttccgaa gagacggacg ccagcagatt atcaaacaca aactt
//
LOCUS       DAOMGY010000390         1793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_76493_length_1793_cov_3.317031, whole genome shotgun sequence.
ACCESSION   DAOMGY010000390 DAOMGY010000000
VERSION     DAOMGY010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76493_length_1793_cov_3.317031"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PLR57_06425"
     CDS             complement(<1..874)
                     /locus_tag="PLR57_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQQ41127.1"
                     /translation="MANRRRRASAARKTLIFILLLASAVALVLYLKSGGAAPTNESSA
                     QIVINEVMISNKGAVPDETGAFPDWVEIYNNTDSSLDISGYGLSDDLLTAAKWTFPEG
                     SVISPRGYLVVFCSGDVTRGKMHAGFKLSASDEVILSTVSGKVVDSITLRAVTSGYSL
                     GRSADDTTTWKEMLPSPGYPNTDEGAAQYLATLSASADESIGVYINEFMPSNASTIIG
                     PDGAYCDWIELYNTNNSEVDLSGYGISDSATQPLKYTLPAGTKIPANGVLLIYCTGRE
                     TPDGATQIEAPFGLA"
     gene            complement(1078..1512)
                     /locus_tag="PLR57_06430"
     CDS             complement(1078..1512)
                     /locus_tag="PLR57_06430"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HQQ41128.1"
                     /translation="MSFVGLFVIIFSGTFGILGLTFLVVGLVFNATTNGKRNRCTSET
                     EGVVTAFQTQFGSSGVRLVYSFSIDGQPMQYLSPYAMNTPNLLVGQKVTVHYDPQNIG
                     NVFIEEERGARTFSRIFVILGAVFVFVALFSAAILLGIAKLP"
BASE COUNT          400 a          504 c          495 g          394 t
ORIGIN      
        1 tggcaagccc gaacggcgct tcaatctgcg tagctccgtc cggcgtttcc ctgccggtgc
       61 agtaaatcag cagcacgccg ttggcgggaa tcttggttcc tgcgggcagc gtatatttca
      121 gcggctgggt tgccgaatcc gaaatgccgt agcccgaaag atcgacttcc gaattgttgg
      181 tattgtagag ttcgatccag tcgcaataag cgccgtccgg ccctatgatg gtagaagcgt
      241 tgctgggcat gaactcgttg atatagacgc cgatggactc gtccgcgctg gctgaaagcg
      301 ttgcaaggta ctgcgccgcg ccttcgtccg tattgggata gccggggctc ggcagcattt
      361 ccttccatgt agtcgtatcg tccgcgctgc ggcccagcga atacccgctc gttaccgcgc
      421 gcagcgtgat gctgtccacc actttgccgg agaccgtgga gaggatgact tcgtccgaag
      481 cggagagctt aaagcccgca tgcatcttgc cgcgtgtcac gtccccgctg cagaacacaa
      541 caagataacc gcgcggcgaa atgacggacc cttccgggaa cgtccatttg gcggcggtga
      601 gcagatcgtc cgataagccg tagccggaaa tatcgaggct ggagtcggtg ttgttgtaaa
      661 tctcaaccca atccgggaac gcgcccgttt cgtcgggtac cgcgccctta ttgctgatca
      721 taacttcgtt gatcacgatt tgggcggaag attcgttggt aggcgccgct ccgccgcttt
      781 ttaaataaag aacgagcgcg acggcgctgg caagcagcaa gataaaaatc agcgtcttgc
      841 gtgctgccga tgctctcctt cttcgattcg ccatgatgaa aatgctcctt ccgcaaacgc
      901 gcaggcgcgc gttttcgcaa cgtccagttt accatacacc cggtcaactg ccaatgacgc
      961 ctcacgatta ttcacaaaat gtccgaaata taaccttatt cgcaccacaa ccctttgtcg
     1021 ggattgaaaa agagccatgt acaggaaaac agctgtaaaa tgccggttta cccgccgtta
     1081 cggcagtttg gcgatcccga gcaaaatggc agccgaaaaa agcgcaacaa aaacgaacac
     1141 cgcgcccaga atgacgaata tgcgcgaaaa cgtccgcgcg ccgcgctctt cctcaataaa
     1201 aacgttgccg atgttctggg gatcgtagtg cacggttacc ttctggccga ccagcaggtt
     1261 cggcgtgttc atcgcatagg gcgaaaggta ctgcatgggc tggccgtcga tggaaaacga
     1321 gtagacgagc cttacgccgc tcgaaccgaa ctgcgtttga aaggcggtga caacgccttc
     1381 cgtttcagaa gtgcagcggt tccgtttgcc gttggtggtg gcgttgaaca ccagcccgac
     1441 gacgaggaac gtcaacccta gaatgccgaa cgttcccgaa aatatgataa cgaacaaacc
     1501 aacaaaactc aatgacattt tattctcctt gtaatatata aacctaaggg gtttccaaag
     1561 gggcgaccgc ccctttggtc ggggtttggg gcagcgcccc aactaaatcg gtcttggggt
     1621 gaaaccccag cggggtttgg ggcggagccc caataaaatc ggtcttgggg cagcgccccg
     1681 ttaggatcgg gtttggggcg gagccccaat cgtcccccta ctcttcttcg ttgcgcagct
     1741 tctcgcgctt ttcgtggttc tccttaatct ttgcttcctg cccctgatcg gaa
//
LOCUS       DAOMGY010000391         1790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_76683_length_1790_cov_3.578098, whole genome shotgun sequence.
ACCESSION   DAOMGY010000391 DAOMGY010000000
VERSION     DAOMGY010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76683_length_1790_cov_3.578098"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1609
                     /locus_tag="PLR57_06435"
     CDS             182..1609
                     /locus_tag="PLR57_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQQ41129.1"
                     /translation="MERYQDESWRRSMRLRVDYNNMMSDQLGKRGLYRREIETMQQQI
                     DTSIANMRLKRPIMRWRELPYNQDEVINSILETAAGIREQFDDFVVLGIGGSALGPIA
                     VQQALNHLHYNDLPREKRNGPRLFVEDNIDPERMAALLDVIDVEKTAFNVVSKSGGTS
                     ETMSQLLIISSLLHEKLGKDISTHLFATTDESKGSLIKIARAENLKTFFIPEGVGGRF
                     SELCPVGLLAAAVCGIDIREMLAGAAYMDEICDSQRDVWKNPAYMLATLQVASIQRGC
                     NISVMMPYADSLRYMADWYAQLWAESLGKRVDWDGNVVNAGQTPVKSLGVTDQHSQVQ
                     LYTEGPFDKVITFLAVDRYRCETMISDGYFDVPDVSFLCGHTQNELIAAELKATEYAV
                     SKSGHLNHTILLPEVNAFTVGQLLFLFELATAFAGELLNINAFDQPGVEEGKKATYAL
                     LGKASYDEKREELAAIAEKNERYII"
BASE COUNT          422 a          510 c          497 g          361 t
ORIGIN      
        1 tatgagagcc gcgcgcgtat gcagcgcatt gtaaaggact ctcctttgcg gtaagcagtt
       61 ttcgtggttt gcaattgcaa caacgtgaaa tttagctgga tttatttgtt cacagatacc
      121 cgcttttatt gaaaaacggt ctatggtatt atgaaatgaa accaaatgga ggcaaaagat
      181 gatggaacgc tatcaagatg aatcctggcg gaggagcatg cgcctgcggg tggactacaa
      241 caacatgatg tccgaccagt tgggcaagcg cggcctgtat cgccgtgaaa tcgaaactat
      301 gcagcagcag atcgacactt caatcgccaa catgcgcttg aaacgcccga tcatgcgctg
      361 gcgcgaactg ccctataatc aggatgaagt gatcaattcc attctggaaa cggcggcggg
      421 tattcgcgaa cagttcgacg atttcgtcgt gctcggcatc ggcggcagcg cgctcggccc
      481 catcgccgtg cagcaggcgc tcaaccacct gcattataac gacctccccc gcgaaaaacg
      541 caacggcccg cgcctgttcg tggaggacaa tatcgacccc gagcgcatgg cggcacttct
      601 ggacgtaatc gacgtggaaa agaccgcgtt caacgttgtc agcaagtccg gcggcacatc
      661 cgaaaccatg agtcagctat tgatcatctc ctcgctgctg catgaaaagc tcggcaagga
      721 tatcagcacg cacctgtttg caacgaccga cgagagcaag ggcagcctga tcaagatcgc
      781 ccgcgcggaa aacctcaaga cgttcttcat tccggaaggc gtcggcgggc gtttttccga
      841 gctctgcccg gtcggcctgc tcgcggctgc ggtctgcgga atcgacattc gcgaaatgct
      901 cgcgggcgca gcttacatgg acgaaatctg cgattctcag cgagacgttt ggaagaaccc
      961 cgcctacatg ctcgcaaccc tgcaggtggc cagcatccag cgcggctgca atatcagcgt
     1021 gatgatgccg tacgcggatt ctctgcgcta tatggcggac tggtacgcac agctttgggc
     1081 ggaatccctc ggcaagcgcg tggactggga cggaaacgta gtcaacgcgg gtcaaacgcc
     1141 cgtcaagagc ctgggagtca ccgaccagca ttcgcaggtg cagctctata cggaaggccc
     1201 gttcgataag gtaatcacct tcctcgccgt cgaccgctac cgctgcgaaa ccatgatcag
     1261 cgacgggtat ttcgacgtgc cggacgtctc cttcctctgc gggcacacgc agaacgagct
     1321 catcgccgcc gaactcaagg cgaccgaata tgccgtgagc aaaagcgggc acctcaacca
     1381 tacgattctt ctgccggaag tgaacgcctt tacggtgggt cagctcctct tcctcttcga
     1441 actcgcgacc gcgtttgcgg gcgaactgct caacatcaac gccttcgacc agcccggcgt
     1501 ggaggaaggt aaaaaggcga cttacgcgct gctcggcaaa gcgagctatg atgaaaagcg
     1561 cgaggaactc gcggcaatcg ccgaaaagaa cgagcgctac atcatctgaa tatgatctcg
     1621 tatcgcgcgc tgacatcagc ggacaaacag caggcaaagg aaatttggga gttgcgtttt
     1681 cacgactccc tttcctttat tgactggtat tttcgcgcgc ggttttctcc cgaaacttcg
     1741 ttttgcgccg aagaggacgg acgcatcgta agcattgtgc acggctcgcc
//
LOCUS       DAOMGY010000392         1788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_76815_length_1788_cov_3.626659, whole genome shotgun sequence.
ACCESSION   DAOMGY010000392 DAOMGY010000000
VERSION     DAOMGY010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76815_length_1788_cov_3.626659"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..96)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            424..>1788
                     /locus_tag="PLR57_06440"
     CDS             424..>1788
                     /locus_tag="PLR57_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQQ41130.1"
                     /translation="MEYSSEAKSMVSKMTLKEKTSLLSGADHWHTKSVERVGLRSVSM
                     SDGPHGLRKEVFSGKKGEKTTLPAVCFPTACLTACSFDRTLLKQLGTALGEAARAESV
                     DILLGPGLNCKRSPLCGRNFEYFSEDPVVSGELAAAYVEGVQSQSVGVSVKHFALNNQ
                     EIRRMIVESVADERAMFELYLAGFERVFQKTRPWTVMCSYNRVKGLQASDNHWLLTDV
                     LRTRFGFDGLVMSDWGAVYDRVKGVSAGMDVEMPYIDPYRDYLAEQAVLNGTLDESDV
                     DRCAERVCELALRARARQPLEQKHDEHHAFARKAATKGAVLLKNEDSLLPLKPGASVA
                     IVGAFAKTPRYQGAGSSKVVPYKIDVPFDELTALGLSTEYAQGYAMESDAPEEALLAE
                     ACRVSAGKDAAILFLGLPDRYESEGYDRDKLNMPQSHIELVRRVKAVNPNVVVVLMGG
                     SVIDM"
BASE COUNT          390 a          478 c          505 g          415 t
ORIGIN      
        1 tcggcaactg gctgccataa acttctgatt ctctcagagt tattaggccc tttagttttg
       61 cgtcgcccgc tttcgcgggg tttgcctttt cgtgggagtt tctctcgaac ctcgcgatta
      121 acgtaccata caaaatgccc cgtgtcaagg tttttgttgc acttttctga ataattatgt
      181 atccgtatgt atcaaatatg tgacattttc gcgttttagt cgtactttac ggaacatagc
      241 atttccattc gacgttcagt gcactcaagt tcagttttta tggtcaactc cattccctgc
      301 ctcgttttgc cgtgatataa tagcaattga atcaatcaac cgcagacgct taaccaaaac
      361 caatcaagct aaatacatca ttattttcgc attttatcaa ttcaataaac cgggaggcta
      421 ggcatggaat attcaagtga agctaaatcg atggtttcca aaatgacgct taaggagaaa
      481 acctctctgc tcagcggcgc ggatcattgg catacaaaat ccgtcgagcg ggtaggctta
      541 cggtccgttt ccatgtccga cgggccgcac gggctgagaa aggaagtctt ttccggcaag
      601 aagggcgaaa aaacgacgct gcccgccgtt tgctttccca ccgcctgcct gacggcctgc
      661 tcgtttgacc gaacgctttt aaagcagctc ggtacggcgc ttggcgaagc cgcgcgcgcg
      721 gaaagcgtgg atattctgct cggccctggt ctcaactgca agcgcagccc gctctgcggc
      781 agaaactttg aatatttcag cgaggatccc gtggtctccg gcgagctggc cgcggcatat
      841 gtggaaggcg tgcagagcca gagcgtcggc gtttccgtaa agcactttgc gctcaacaac
      901 caggagatac ggcgcatgat cgtggaatcc gttgcggacg agcgcgccat gttcgagctc
      961 taccttgccg ggtttgagcg cgtgtttcaa aaaacgcgcc cctggacggt catgtgctcc
     1021 tataaccgtg tcaaaggcct gcaggcgagc gataatcatt ggctgctgac agatgtcctg
     1081 cgtacgcgtt ttggctttga cgggcttgta atgtccgact ggggcgcggt atacgaccgc
     1141 gttaaaggcg tttccgcggg gatggatgtt gaaatgccat atatcgaccc gtaccgcgac
     1201 tatctcgcgg agcaggcggt attgaatgga acgctggacg aaagcgacgt tgatcgctgc
     1261 gccgaacgcg tctgcgagct ggcgcttcgt gcgcgcgcgc gtcagccgct tgagcagaag
     1321 cacgacgaac accacgcgtt cgcacgaaaa gccgccacga agggagcggt gctgcttaaa
     1381 aacgaagatt cgctgctccc gcttaagccc ggcgcaagcg tcgccatcgt gggcgcgttt
     1441 gcaaaaacgc cgcgctatca gggcgcgggc agttcgaagg ttgtgccgta taaaatagac
     1501 gttcctttcg acgagctgac agcgctcggc ctaagcacgg aatatgcgca ggggtatgca
     1561 atggagtccg acgcgccgga ggaagcgctg cttgccgagg cctgccgcgt ttcagcgggc
     1621 aaggacgccg caatcctgtt tctcggtctg ccggatcgct acgagtccga agggtacgac
     1681 cgcgacaagc tgaatatgcc gcagagccat attgagctcg tcaggcgcgt caaggccgtc
     1741 aacccgaacg tcgttgtcgt gctcatgggc ggttcggtga tcgatatg
//
LOCUS       DAOMGY010000393         1788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_76817_length_1788_cov_3.504905, whole genome shotgun sequence.
ACCESSION   DAOMGY010000393 DAOMGY010000000
VERSION     DAOMGY010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76817_length_1788_cov_3.504905"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..349
                     /locus_tag="PLR57_06445"
     CDS             41..349
                     /locus_tag="PLR57_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41131.1"
                     /translation="MRNLIGSSIPANYFRGAEAVGGKLRFDETGMTFQSHSFNISTGE
                     TRIDYSDILLIKERNTLGIVRNGISVFTENGFEHKFVVNGREEIIEFLTMQSKKHREK
                     "
     gene            complement(540..>1788)
                     /locus_tag="PLR57_06450"
     CDS             complement(540..>1788)
                     /locus_tag="PLR57_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41132.1"
                     /translation="TPEPRVIEVEMDQELQGVKGAVVVAKRITYVQNQSISITLVIQN
                     NDGDIYLIAPGFASVNGWGFVLYPKVKSDNISIFQNNAKTSITLVAELDETTDQLMNI
                     TQIQNVTVDVALMRFDGSLTMYAKSGLYTNPDCPEDYVQPHPVLSDSSVAVSKFDEKT
                     FVSADLNDIFWIDKEVYDQNTQRLFYIMGGSSLYGKPDALLANSGVANLALDGTTTGR
                     NAKFYLPNGGYTVFSFDLSDSNGFDPELQRRTVSFLASDTPDHFVGGFLWADFTPDAL
                     EQGYIRSLDESGVVWKETSTYKLIYKGFNYVSAVRPSYGDGALEAHNCIEFLLVNKSQ
                     DDALGVYTDKMSINGTDLSPRITKSVIPPQCAGIVQIDVPSRWWPDGMKNMEDGRFAL
                     TLKILKPGGVVIDTIEQVIEPSK"
BASE COUNT          466 a          458 c          420 g          444 t
ORIGIN      
        1 cttatttcat aagaaatgat tataaaggag cttgaaagaa atgagaaacc tgattggcag
       61 cagcatcccg gcgaactatt ttcgcggcgc ggaggccgtt ggcggcaaac tgcgctttga
      121 cgaaaccggc atgacgtttc agtcccacag cttcaacatc agcaccggcg aaacgaggat
      181 cgactacagc gacatccttt taatcaagga gcgcaacacg ctgggcattg tccgcaacgg
      241 catttcggtg ttcaccgaga acggctttga gcataagttc gtcgtcaacg gccgcgagga
      301 gattattgag tttctgacga tgcagtcgaa aaaacatcgg gagaagtaaa cgcagctcaa
      361 agggattata agtgcagaga cgaacaagcc cgcaagttga ttgcgggctt gacgcgattg
      421 cttatacatt catttattca gaaaactcta ttggcgtaat tcctgttttc gaatgaagta
      481 aatgaagcgc cgaccggttc cgaatgagcc cttgctcctg tcaggaagca aaagtaggat
      541 tacttcgaag gttcgattac ctgctcaatc gtatctatca cgactccgcc aggtttcaaa
      601 atctttagcg tcagcgcaaa ccgtccgtct tccatattct tcataccgtc cggccaccaa
      661 cggcttggaa cgtcaatttg tacaatcccg gcacactgag gcggaatgac gctttttgtg
      721 attcgcggag aaagatctgt tccgtttatc gacatcttat ccgtgtaaac acccagcgca
      781 tcgtcttgcg acttattgac cagaaggaat tcaatgcagt tatgcgcttc cagcgcgccg
      841 tccccgtaac ttggcctgac ggctgaaacg taattgaatc ccttgtagat cagcttatat
      901 gtcgaggttt ccttccacac aacaccgctt tcatccaacg aacgaatgta cccctgctcc
      961 agcgcatccg gtgtaaagtc ggcccataaa aagccgccga caaaatgatc cggcgtatcc
     1021 gatgccagga acgatactgt gcggcgctgc agttccggat cgaaaccgtt ggaatccgac
     1081 agatcaaacg aaaagacggt atacccgccg ttggggaggt aaaatttagc gtttctcccg
     1141 gtcgtggttc cgtccagggc cagattggcc acgccggagt ttgccaacag cgcgtcgggt
     1201 tttccataaa gcgaggaacc gcccataata tagaacaacc gctgcgtatt ttggtcgtag
     1261 acctccttgt cgatccagaa tatatcgttc agatccgcgc tcacgaaggt tttttcgtca
     1321 aatttggata cggcaaccga gctgtcgctc aataccggat gcggctgtac ataatcttca
     1381 gggcaatccg ggttcgtgta aagcccggat tttgcataca tcgtcaaaga cccgtcaaac
     1441 cgcatcaaag ccacatctac tgtaacgttc tgaatttgcg taatattcat gagttgatcc
     1501 gtggtttcgt ccagttccgc tacaagggtg atgctcgttt ttgcattgtt ttgaaagata
     1561 gaaatattgt ccgatttcac ctttggataa agcacgaatc cccacccgtt gacagacgca
     1621 aaacccggcg caatcaaata tatatcacca tcgttgtttt ggattacaag cgtaatagaa
     1681 atactctgat tttgaacgta agtaatcctt ttggcgacga ccaccgcgcc cttaacgccc
     1741 tgaagttcct gatccatctc cacttcaatc acgcgcggtt cgggggtg
//
LOCUS       DAOMGY010000394         1786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_76975_length_1786_cov_2.883304, whole genome shotgun sequence.
ACCESSION   DAOMGY010000394 DAOMGY010000000
VERSION     DAOMGY010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76975_length_1786_cov_2.883304"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLR57_06455"
     CDS             complement(<1..211)
                     /locus_tag="PLR57_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HQQ41133.1"
                     /translation="MKIAIDAMGGDNAPQAVIAGVQLVLPELPADAELLLFGDENVLG
                     SDIADAHINDPRVKIVHTSQVIGCDE"
     gene            complement(477..677)
                     /gene="rpmF"
                     /locus_tag="PLR57_06460"
     CDS             complement(477..677)
                     /gene="rpmF"
                     /locus_tag="PLR57_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQQ41134.1"
                     /translation="MAVPKRKTSKARRDSRRAANVNLTAPQLNECPQCHALVPSHTVC
                     KTCGYYDGKQVVDMEKKEKKNA"
     gene            complement(720..1190)
                     /locus_tag="PLR57_06465"
     CDS             complement(720..1190)
                     /locus_tag="PLR57_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQQ41135.1"
                     /translation="MRIRVANAIKQTGDEFPFTCSEQWEPVSYGERVLTFSEPVRVEG
                     TYVFDDKAFTVTAKAEAALLSECARCLKAFTERISVSFSERFVKDNPDSEDDETYPYT
                     GDELDLGKAVLDNLLLELPIASVCREDCKGLCPVCGADWNVTECQCTKTEAWEN"
     gene            complement(1400..>1786)
                     /locus_tag="PLR57_06470"
     CDS             complement(1400..>1786)
                     /locus_tag="PLR57_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HQQ41136.1"
                     /translation="PGTASTCMYYTGLDPFTMEPVYVAKSREEKAMQRALMQCHIRKN
                     WPLIRKALQQAERDDLIGNGGGCLVPPDYQSTQTDGYRPKQSGAKPSQRSGGGKRGTD
                     RKPSKNNVYGRPSKPGGSSGRGGKRG"
BASE COUNT          331 a          526 c          464 g          465 t
ORIGIN      
        1 gttcgtcgca gccgatcacc tgcgaggtat ggacgatttt tacccgcgga tcgttgatat
       61 gcgcgtccgc aatgtcgctg cccagcacgt tctcatcgcc aaagagcagc agctccgcgt
      121 ccgccggaag ctccggcaag acgagctgta cccccgcgat aaccgcctgc ggcgcgttgt
      181 cgccgcccat ggcgtcgatt gcaatcttca ttcgttcatt ctcctgttcg cgcggctgat
      241 cgccgcagtc ttcaccccat ccccatgagg cgtttataag gattgtatga ttccgtccgc
      301 aagcgttctc ttttgcggat gactaattat acgaaaatta cactttcgat gcaacgccaa
      361 aagcgttcgg ctccgcttac gacacgcgca atatgaaaaa aaagcaaatg aaaaagcctt
      421 tccgaatgga aaggcttttt cataaactgg taatcatatc gccgagggat ggatgatcag
      481 gcgttcttct tttccttttt ctccatgtcg acaacctgtt tgccgtcgta atacccgcac
      541 gttttgcata ccgtatggga cggtacaagc gcgtggcact gcgggcactc gttgagctgc
      601 ggcgcggtca ggttcacgtt ggccgctctg cgcgaatcac gccttgcctt cgacgttttt
      661 ctcttgggta ctgccatgat aatccctcct cttcttcttc agatagcgcg ttggaaaaac
      721 tagttctccc aggcttcggt ttttgtgcat tggcattcgg ttacattcca gtccgcaccg
      781 caaacggggc aaaggccctt gcagtcctcc cggcagacgc tcgcgatggg aagctccaaa
      841 aggagattgt ccagcacggc tttgccgagg tcgagctcgt ctccggtgta tggatacgtc
      901 tcgtcgtcct ccgagtccgg attgtctttt acaaaccgct cggaaaacga aaccgaaatg
      961 cgttctgtaa acgcttttaa gcagcgggcg cattccgaaa gcagcgccgc ctcggctttt
     1021 gccgtaacgg tgaatgcctt gtcgtcgaat acgtacgtcc cttccaccct gacgggttcg
     1081 gaaaacgtca gcactctttc tccgtaggag accggttccc attgttcgga acaggtaaac
     1141 ggaaattcgt cgcccgtttg cttgatcgcg ttcgcaacac gaattcgcat agttctcacc
     1201 gcctgtcagc cggtaactcc ggcgttgttt tgaatctacc agcgcgcatg aaaaccatcg
     1261 cgcgtttcca cgcaacattg cttattttat cggctgcacg gttgtttgtc aagaacaagc
     1321 gtgctctccc gtgcttgttt ctcttacttt ttctgttgta agcgccctga taagcgagcg
     1381 tatttgccgg attacgaggc tatccgcgtt tgccgcccct gccggagctt ccgccgggct
     1441 tgcttggtct tccatagacg ttgttcttgg aaggctttcg atctgtgccg cgcttgccgc
     1501 ctccgcttct ttgagacggt tttgcaccgc tctgcttcgg acggtaaccg tcggtctgcg
     1561 tgctctggta gtccggcgga acgaggcagc ctccgccgtt gccgatcaga tcgtcccgct
     1621 ccgcctgctg cagcgccttg cggatcagcg gccagttttt gcgaatgtgg cactgcatca
     1681 gcgcgcgctg catggccttt tcctcgcggc tctttgcaac gtacaccggt tccatggtaa
     1741 acggatcgag cccggtataa tacatgcagg tgctcgcggt gccggg
//
LOCUS       DAOMGY010000395         1786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_76976_length_1786_cov_2.816291, whole genome shotgun sequence.
ACCESSION   DAOMGY010000395 DAOMGY010000000
VERSION     DAOMGY010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76976_length_1786_cov_2.816291"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLR57_06475"
     CDS             <1..322
                     /locus_tag="PLR57_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ41137.1"
                     /translation="SRLIEKMMLWANVTKEELEERKIIETGFGQPLSWNYYCYAKQHP
                     IESWSSPASLLYGSLESLTDRSDIDAFAERFHCDLTVMENGEHWFHTEEQLAVLNDWI
                     AERV"
     gene            386..1093
                     /locus_tag="PLR57_06480"
     CDS             386..1093
                     /locus_tag="PLR57_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQQ41138.1"
                     /translation="MVLILASFLAALISGAAGFGGALLLLPVITYYIGAEAAVPVITL
                     AQLIGNLARMASGFRQIKWSAVGWFCLAALPLSALGAFGFTVLPKDIVTRCIGGALIL
                     LVVFKMCKLFELKGSRATLLVGGAVTGGLSGLAGSGGPIGAAVFLSLGLSPVAYIASE
                     AATAVAMHALKTVVYSKLASLMIETLLLGLAMGAAMVAGTFAANRFIRRMEKGRFQTF
                     VAVLLCVVGLYMLIFGA"
     gene            complement(1159..>1786)
                     /locus_tag="PLR57_06485"
     CDS             complement(1159..>1786)
                     /locus_tag="PLR57_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQQ41139.1"
                     /translation="FRQLELDRFSIVGLSMGGGLAIEYAAQHTEQVVSMALFEPGGLG
                     ETVTGQLFTYCYIHTPGMLRLLSKRYVKMGRPQIEKTLRSLYTGTSAPTDPERLITIL
                     QDEIRGKFECGENDMDDWQLSGIGAFQLKWNLLGTIPQIQCPTLWLRGENSVLVKQSE
                     MERAAALARQGGSEATLTTVPNAGHMLPLEQPETANRAVLEFLNRTTK"
BASE COUNT          335 a          504 c          521 g          426 t
ORIGIN      
        1 gtcccggctg atcgaaaaga tgatgctctg ggcgaatgtc acgaaagagg aactagagga
       61 gcgaaagatc attgaaacgg gctttggcca gccgctgagc tggaactatt attgctatgc
      121 aaagcaacat cctatcgaga gttggagcag tcccgccagc ctgttgtacg gaagtcttga
      181 gagcctgacc gacagaagcg atatagacgc gtttgccgag cgcttccact gcgatttgac
      241 cgtgatggaa aacggcgagc attggtttca tacggaagaa cagttggccg ttttgaacga
      301 ttggatcgcc gaacgcgtat aagccgcatt tatctttgct tgcctgaacg taaactggag
      361 agagaatgag gagccgatta tgtggatggt gctgatactg gcgagctttc tcgcggcgct
      421 gatctcgggc gctgcgggct ttggcggcgc gctgctgctc ttgccggtca tcacgtacta
      481 tatcggcgcg gaagcggctg ttcccgtgat tacgctggct cagctcatcg gcaacctcgc
      541 gcggatggct tcgggattcc ggcagatcaa gtggagcgcg gtcggctggt tctgccttgc
      601 cgccctaccg ctctctgcac tcggcgcttt tggctttacc gttttgccaa aagatatcgt
      661 aacgcgatgc atcggcggcg cgctgattct tcttgtcgtg ttcaaaatgt gcaagctctt
      721 tgagctcaaa ggcagccgcg cgacgctgtt ggtcggcggc gcggtcaccg gaggactctc
      781 cgggctggcg ggcagcggcg ggccgatcgg agccgcggtg tttctctcgc tggggctttc
      841 ccccgtcgcc tatatcgcaa gcgaagcggc gaccgccgtg gccatgcatg cgctcaaaac
      901 ggtcgtttac tccaagcttg cttccctgat gatagaaacg cttttgttag ggcttgccat
      961 gggcgccgcc atggttgccg gaactttcgc cgcaaaccgg ttcatccggc gcatggagaa
     1021 aggcaggttc cagacgtttg ttgcggttct gctctgcgtg gtcggccttt atatgctgat
     1081 cttcggggct taatttttgt ctctctaact actgtatacg caaaacccgc cttaatcggt
     1141 gggctttttt gtcgcgctct atttcgttgt tctgttcaaa aactcaagta cagcccggtt
     1201 tgccgtttcc ggctgttcca gcgggagcat gtgccccgcg ttcggaaccg ttgtcagcgt
     1261 cgcctcgctg ccgccctgac gcgcaagcgc agccgcgcgc tccatctcgc tctgcttgac
     1321 gagcacgctg ttttcgcccc tgagccagag cgtagggcat tgtatctgcg gaatcgtacc
     1381 gagcaggttc catttcagct ggaacgcgcc gataccgctg agctgccagt cgtccatatc
     1441 gttttcgccg cactcgaact tgccacgaat ctcgtcctgc agaatcgtaa tcagccgctc
     1501 cgggtcggtc ggcgcagaag tgcccgtata gagcgagcga agcgtttttt cgatttgcgg
     1561 gcgacccatt ttgacataac gcttgctcag cagacgcagc atgcccggcg tatgaatata
     1621 gcagtaggta aacagctgtc cagtgaccgt ttcacccagc cctcccggct caaacagcgc
     1681 catggaaacc acctgttcgg tgtgctgtgc cgcatattca atcgccagtc caccgcccat
     1741 agagaggccg acgatgctga acctgtcgag ttccagttgg cggaac
//
LOCUS       DAOMGY010000396         1785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_77043_length_1785_cov_3.055491, whole genome shotgun sequence.
ACCESSION   DAOMGY010000396 DAOMGY010000000
VERSION     DAOMGY010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77043_length_1785_cov_3.055491"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLR57_06490"
     CDS             <1..608
                     /locus_tag="PLR57_06490"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HQQ41140.1"
                     /translation="AASGADGVSSVPLPGASHAQQMRYYRALAKAATIPVILYYMPQP
                     GVNFSLDNVLETLTVPGVTGVKSSTNDFFFTQQLVAEKPAGCAVFNGKDEYLAPAVFH
                     GADGGIGMWATVFPAAYAGIYHAAKAGDYARAFDLQKTLNALCVIVMHKGLLQSFACI
                     LHELGLHDRVFRAPAPQYDAAFAKDFFAEARPLVEKLASVL"
     gene            644..1552
                     /locus_tag="PLR57_06495"
     CDS             644..1552
                     /locus_tag="PLR57_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQQ41141.1"
                     /translation="MNRIPKTEISVSPVTLGTMTYGSPVAFDDAVKLTRYALSQGVNH
                     IDTANMYEGYNRYAGSAGGVAEEIVGSAIKGMPRGSVIVSTKVGMKVGDAPEDEGTSA
                     NAIEKQLDASLRRMKTDYIDLYYLHRYDANAPLPEILTALQKAVFAGKIRYYGVSNYS
                     AEQLSALLKAADELGLPRPVVCQPPLSLLKQDALSALIPLCAAEDLAVIPYQIYQGGL
                     LTGKYKRGAEPPKGSRAEEKPAWLAAPDDTIYDKLEAIEQSAKARGISMSAYALRWTL
                     EQPAVVSAIVGVKSDRQVDDALAALR"
     gene            1588..>1785
                     /locus_tag="PLR57_06500"
     CDS             1588..>1785
                     /locus_tag="PLR57_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41142.1"
                     /translation="MTVQYEDALKGFREVGILPGDTVLLQSAMRPFGFVEGAGKTIGM
                     ALKEALGNAGATSVPSSLPSAP"
BASE COUNT          385 a          526 c          534 g          340 t
ORIGIN      
        1 ccgcggcgag cggggcggac ggggtatcgt ccgtcccgct gccgggcgct tcccacgccc
       61 agcagatgcg gtattaccgc gcgctggcaa aggcggcgac cattcccgtg attctctact
      121 acatgccgca gccgggcgtg aacttttcgc tcgacaacgt gctcgaaaca ctcaccgttc
      181 cgggcgtaac gggcgtaaaa tcctccacga acgatttttt ctttacccag cagcttgtcg
      241 cggaaaaacc cgccgggtgc gctgtgttta acggcaagga cgaatacctt gctcccgccg
      301 tattccacgg cgcggacggc ggcatcggca tgtgggcgac cgtgtttcca gcagcgtacg
      361 cgggaattta ccacgccgcg aaagcggggg attacgcgcg cgcgttcgat ctgcaaaaaa
      421 cgctcaacgc gctctgcgtc atcgtcatgc ataaaggatt attgcagagc tttgcctgta
      481 ttctgcacga gctgggtttg cacgatcggg tgttccgcgc gcccgcgccg cagtatgatg
      541 cggcgtttgc aaaagacttt ttcgccgaag cgaggccgct cgtcgaaaag ctggcctcgg
      601 ttttgtaagt accgtattgt acaaaatggg agaaaactga caaatgaacc gtataccaaa
      661 aacagagatt tccgtgtccc cggtcacgct gggcacgatg acctacggct cgccggtcgc
      721 ttttgacgac gcggtgaagc tcacgcgcta tgcgctttcg cagggcgtga atcatatcga
      781 caccgccaac atgtacgaag gctataaccg atacgcgggc agcgcgggcg gggttgccga
      841 ggaaattgta ggcagcgcca tcaagggcat gccgcgcggg agcgtaatcg tctctaccaa
      901 agtgggcatg aaggttggcg atgctcccga ggacgaaggc acgagcgcga atgcaattga
      961 aaagcagctc gacgcgagcc tccggcgcat gaagaccgat tatatcgacc tgtattacct
     1021 gcaccgctac gatgccaacg cgccgcttcc ggaaattctt acagcgctgc aaaaagcggt
     1081 attcgcgggc aaaatccgct actacggcgt cagcaactac tccgccgaac agctttccgc
     1141 gctcttaaaa gcagcggacg aattgggact tccgcgtccg gtcgtctgcc agccgccgct
     1201 gagcctgctc aagcaggatg cgctttccgc gctgattccc ctttgcgcgg cggaagacct
     1261 agcggtcatc ccttaccaga tctatcaggg cggactgctc acgggcaagt acaagcgcgg
     1321 cgctgaaccg cccaagggtt cgcgcgcgga agaaaaaccc gcttggcttg ccgctccgga
     1381 cgatacgatt tatgataaac tggaagcgat tgaacagagc gcaaaggcgc gcggcatttc
     1441 gatgagcgca tacgccctgc gctggacgct ggagcagccc gccgtggttt ccgccatcgt
     1501 gggtgtcaag agcgaccgtc aggtggacga cgcgctcgcc gcgctccgct gagaacgatt
     1561 ctgatcaagt aaggagataa ctgctaaatg acagttcaat atgaagacgc gttaaaaggg
     1621 ttccgcgaag ttggaattct tccgggagat accgtactgc tccagtccgc catgcgcccg
     1681 ttcggctttg tggaaggcgc gggcaaaacg atcggcatgg cgctgaagga agcgctgggt
     1741 aacgcgggcg caaccagcgt gccctcgtcg ttacccagcg ctccc
//
LOCUS       DAOMGY010000397         1784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_77099_length_1784_cov_3.136495, whole genome shotgun sequence.
ACCESSION   DAOMGY010000397 DAOMGY010000000
VERSION     DAOMGY010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77099_length_1784_cov_3.136495"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLR57_06505"
     CDS             <1..575
                     /locus_tag="PLR57_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41143.1"
                     /translation="DKPAMPAEPEKRARAVARQASTPAQKQARPPKVQRVVKNGVEKE
                     EPQAAQKPAQANGEDWFDELLVNLCERERQGYTPVKTESAPEVAARETEPATAEEPVP
                     PNDFSLRSRDEQPKPAEQIVFKHATEEPAWQEPEAEEPQPAEAAAQEPDEPAVEVRKA
                     YAAAPEQANEPGEPEQEEFSEPEQPSGESE"
     gene            582..>1784
                     /locus_tag="PLR57_06510"
     CDS             582..>1784
                     /locus_tag="PLR57_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HQQ41144.1"
                     /translation="MPKQKQLFPPVSRAELNARNIGQPDFIFVTGDAYIDHPSFGAAI
                     ITRLLESKGYSVALIVQPDWHSAEAFAALGKPRLAFLVSSGNLDSMVNHYSVSGARRD
                     TDAYTEGGAAGKRPDHATIVYANRCREAFPNVPLILGGIEASLRRFAHFDYWDYRVRH
                     SILYDACADLLVYGMGERAIVQIADALKAGRPVSELTDIPGTCARVSAPLEGAYVLLP
                     SFTEVASDKQKYCEAFVLQTREQDAVRGKRLLQPHEKGFLLCNPPAMPLSEAELDEVY
                     ALPFTRRPHPSIKEFVPAIEEVSFSITSARGCFGNCNFCALTFHQGRVVTSRSHESIL
                     DEAERLTHEPDFKGYIHDVGGPTANFRHPACAKQLRSGVCADRECLGFSACKNIDASE
                     YDYTLLLKK"
BASE COUNT          388 a          555 c          546 g          295 t
ORIGIN      
        1 cggacaaacc tgccatgccc gccgaaccgg agaagcgcgc cagagcggtt gcgcggcagg
       61 ccagcacacc tgcgcaaaag caggcgcgcc cgcccaaggt acagcgcgtc gtaaagaacg
      121 gcgtggagaa ggaagagccg caggctgcgc agaagcccgc gcaggccaac ggcgaggact
      181 ggttcgacga gctgctcgtc aacctctgcg aacgcgaacg gcagggctac acgccggtca
      241 agacggaatc ggctccggaa gttgccgcaa gagagacgga gcctgcaacg gcggaagaac
      301 ccgtgccgcc aaacgatttt tcactgcgct cgcgcgacga acagccgaag ccggcggaac
      361 agatcgtgtt taaacatgcg acggaagaac ccgcctggca ggaacctgaa gcagaagagc
      421 cgcaaccggc ggaagctgcc gcgcaggaac cggacgaacc agccgtagaa gtacgtaaag
      481 cgtacgcggc ggcgcccgaa caggcgaatg aacccggtga accggagcaa gaggaatttt
      541 cagaaccgga acagccttcc ggcgaaagcg aatgagatag aatgcccaaa caaaaacagc
      601 ttttcccgcc cgtctcccgc gcggaactca acgcgcggaa tatcgggcag cccgatttta
      661 tatttgtcac cggggatgcg tatatcgacc atcccagctt tggcgcggcc atcattacgc
      721 gcctgctcga aagcaagggc tacagcgtgg cgctgatcgt ccagccggac tggcacagcg
      781 cggaagcgtt cgcagccctt ggcaaaccgc gccttgcgtt tctggtgtcc tccggaaatc
      841 tcgattccat ggtgaaccat tactccgtct ccggcgcgcg ccgggatacg gacgcgtata
      901 ccgaaggagg agcagcgggc aagcgccccg accacgccac gatcgtctac gccaaccgct
      961 gccgggaagc atttccgaac gttccgctga ttctcggcgg cattgaagcg agtctgaggc
     1021 ggtttgcgca cttcgactac tgggattacc gcgtgcggca ttccatcctc tacgacgcat
     1081 gcgccgacct gctggtctac ggcatgggcg agcgtgccat cgttcagatt gcggatgcgc
     1141 tcaaagcggg cagacccgtt tccgaactta ccgatatacc cggcacctgc gcgcgcgtct
     1201 cagcgccgct ggaaggggcg tatgttctgc tgccgagctt tacggaggtc gcctccgata
     1261 aacaaaagta ctgcgaagcg ttcgttctgc aaacgcggga acaggacgca gtccgcggca
     1321 agcgtctgct ccagccgcac gaaaaggggt ttttgctctg caatccgccc gccatgccgc
     1381 tttccgaagc ggagctggac gaagtctacg cgcttccgtt tacgcggcgg ccgcaccctt
     1441 ccattaagga atttgttccc gccatcgagg aggtatcgtt ttccatcacc tccgcgcgcg
     1501 gttgcttcgg caactgcaac ttctgcgcgc tgacgttcca tcaggggcgg gtggtgactt
     1561 cgcgttcgca cgagtccatt ctggacgaag cggagcggct gacccacgag ccggacttta
     1621 agggatacat tcacgacgtt ggcggcccga cggcgaactt tcgccatccg gcctgcgcaa
     1681 agcagctcag aagcggcgtc tgcgcggatc gggagtgcct gggcttttcc gcctgcaaaa
     1741 acattgacgc gagcgagtac gactatacgc ttctgctcaa gaag
//
LOCUS       DAOMGY010000398         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_77163_length_1783_cov_3.134838, whole genome shotgun sequence.
ACCESSION   DAOMGY010000398 DAOMGY010000000
VERSION     DAOMGY010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77163_length_1783_cov_3.134838"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..825
                     /locus_tag="PLR57_06515"
     CDS             97..825
                     /locus_tag="PLR57_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HQQ41145.1"
                     /translation="MLNEFTRTELLLGGEAMERLSAARVAVFGIGGVGSFAAEALARA
                     GIGALDLFDDDKVCLTNINRQLIATHKTIGQKKVEVMRERILDINPKCNVTAHECFYG
                     AENAEEYDLSVYDYIVDAIDTVSSKLLLIERAFQANAPVISCMGAGNKLDPTRFTVAD
                     IYATSVCPLARVMRRELKKRGISALKVVYSTEEAIQPQETEELRRGSRRAVPGSVSFV
                     PPVAGFILASEVVKDLIARPAQGQ"
     gene            complement(886..>1783)
                     /locus_tag="PLR57_06520"
     CDS             complement(886..>1783)
                     /locus_tag="PLR57_06520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HQQ41146.1"
                     /translation="PLGEGEFSAVYSVRAGERDYVVKLAPPEHVPILTYERDIMASEL
                     FWYGVMRDQTSIRVPEVYAADDSNSLVPVNYFIMELLPGAPLNHAALMPQEKSACASE
                     LARMAARMHKVKNDRFGYPQNGLHDSWYAAIRQMTENLLSDCKRVRRRSRRGERLLRE
                     IERNRAILENVPCCMVNFDIWEPNIIVSRENGALHLAWIDPERSFWGDRMADFVCLDF
                     MSPLEKKTAALAAYNSEADDPVLATPEETVRWAVMQAYLALIMETEKYYRYSLVSFGW
                     WRNVVAAGMLYRSAFRALREHQ"
BASE COUNT          367 a          525 c          525 g          366 t
ORIGIN      
        1 cctgccggac ggcggggaac gctatacctc cacgccgctg tttggggact gaactactgt
       61 tttacacgat ataaaagccg tgaaaggaac ttccgcatgc tgaacgaatt tacgcgaacc
      121 gaactcctgc tgggtgggga agccatggag cgcctttccg ccgccagagt ggccgttttc
      181 ggcatcggcg gggtcggctc gtttgcggcg gaagcgcttg cacgcgcggg catcggcgcg
      241 ctcgacctgt ttgacgacga caaggtctgc ttaaccaaca tcaaccgcca gctgatcgcc
      301 acgcacaaga ccatcggtca aaagaaggtc gaggtcatgc gggaacggat tctggacatc
      361 aaccccaagt gcaatgtgac cgcgcacgaa tgcttctacg gcgcggaaaa cgcggaggaa
      421 tacgatcttt cggtatacga ctatattgtg gacgccatcg acaccgtttc ctccaagctg
      481 ctgctcatcg agcgcgcgtt tcaagcaaac gcgccggtga tcagctgtat gggcgcgggc
      541 aacaaactcg acccgacgcg ctttacggtt gcggatatct atgctacttc cgtctgcccg
      601 ctggcgcgcg tgatgcggcg ggaactgaaa aagcgcggca tttccgcgct caaggttgtg
      661 tattccaccg aggaggcgat tcagccacag gaaactgagg aactgagacg tggctcgcgg
      721 cgcgcggtgc cgggcagcgt ttcgttcgtg ccgccggtcg cggggttcat cctcgcttcg
      781 gaagtggtaa aggatctcat tgcccgtcca gcgcaaggac aatagaacag cgaaacaaac
      841 gtgtaagagc ggcttttgcc gctcttttgc cgtccgtatt tttgattatt gatgttcgcg
      901 cagcgcgcga aacgccgagc ggtagagcat ccctgcggca actacattgc gccaccaacc
      961 gaatgacacg agcgaataac ggtagtactt ctccgtttcc ataatcagcg caagatacgc
     1021 ctgcatcacc gcccagcgaa cggtctcttc gggcgtggcg agcacgggat cgtccgcctc
     1081 gctgttatag gccgcgagcg cggctgtttt cttttcgagc gggctcataa aatccaaaca
     1141 gacaaagtcc gccatccggt cgccccagaa gctgcgctcg gggtcgatcc aggcaaggtg
     1201 caatgctccg ttttcgcggc tgacgatgat gttcggttcc cagatgtcga agttgaccat
     1261 gcagcaggga acgttttcca gaatggcacg gtttcgctcg atttcgcgca gaagccgctc
     1321 gccgcgtctt gaacggcggc gcacgcgttt gcagtcagag agcagattct ccgtcatctg
     1381 gcggattgcc gcataccagc tgtcgtgcag cccgttttgc gggtagccga agcggtcgtt
     1441 tttgactttg tgcatccgcg ccgccatcct ggcgagttct gaagcgcagg cggacttctc
     1501 ttgcggcatt aacgccgcgt ggttcaacgg cgctcccggc agcagctcca tgatgaagta
     1561 attcacgggc acgagcgaat ttgaatcgtc cgccgcgtag acttccggca cgcggatgct
     1621 cgtttgatcg cgcataacgc cgtaccagaa cagctcggaa gccatgatat ctcgctcata
     1681 tgtcaaaatg gggacatgct ccggcggcgc gagcttgacg acgtaatccc tctcgcccgc
     1741 gcgcacggaa tacacggcgc tgaactcccc ttcgccaagc gga
//
LOCUS       DAOMGY010000399         1782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_77260_length_1782_cov_2.475970, whole genome shotgun sequence.
ACCESSION   DAOMGY010000399 DAOMGY010000000
VERSION     DAOMGY010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77260_length_1782_cov_2.475970"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..780)
                     /locus_tag="PLR57_06525"
     CDS             complement(148..780)
                     /locus_tag="PLR57_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HQQ41147.1"
                     /translation="MQNTQGVAPYTTVKTAGESEIVISKSRFLGYCFPVSSEAEAAER
                     IAEIRKRHWDARHVCYAFRLLGGVARSTDDGEPSGTAGAPILNVLTQSGLENVLCAVV
                     RYFGGVLLGTGGLVRAYGRTASEAVAHAGTLSYRICRRMRILVSYSSYAVLEPLTKAL
                     AYPCEAEFSDMVTLRCTIPVEECERFCRTVTDKTDGSAKLELLEQTLCAV"
     gene            complement(759..>1782)
                     /gene="tyrS"
                     /locus_tag="PLR57_06530"
     CDS             complement(759..>1782)
                     /gene="tyrS"
                     /locus_tag="PLR57_06530"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQQ41148.1"
                     /translation="LGNGTAFIGDPSGKTDMRTMMSKETLAYNASCFKAQMGRLLDFS
                     EGKAQIVYNGDWLYPLNFLDFMRDIGVYFSVNRMLTAECYKQRMEKGLTFFEMSYMLM
                     QSYDFLELNRRYGCILQMGGDDQWSNILNGADLIRRKEQKPAYALTTSLLTTSDGKKM
                     GKTEKGAVWLDPNKCSVFDFYQYWRNVGDQDVEKCLSLLTFIPMEEVKRLGALPGEQI
                     NEAKRTLAYTITAQVHGEEEAKKAQTAAESLFAGGSDQSNIPTYEISADEIAQSGLRV
                     TDLLVLAKLAVSKSDARRLIEGGGVFVGERKVESVNESVDPEEISGEGVILRKGKKGY
                     CRIRRA"
BASE COUNT          346 a          549 c          473 g          414 t
ORIGIN      
        1 tggttacgtt gatttcatcc gcgcgcgcgg aaggcatcca taggataagg cacagcacaa
       61 cggcaaagac cgtctcggcc gatttagcga ttctgttcca ttttgcttgt ttcgaggcct
      121 gtgccttacg cttcatatcg accttcctca aaccgcgcac agcgtttgtt ccagcagttc
      181 cagttttgca gagccgtccg ttttatccgt taccgttcgg caaaaccgct cacattcttc
      241 cactggaatg gtgcagcgca gtgttaccat atcgctgaat tccgcttcgc agggatacgc
      301 cagcgctttt gtcagcggct ccagcacggc atagcttgaa taggaaacaa gaattctcat
      361 gcgccgacag atgcggtagc tgagcgttcc cgcatgagcg accgcttccg aagcggtcct
      421 gccgtacgcg cggacgagtc cgcccgttcc cagcagcacg ccgccgaaat accgcaccac
      481 cgcgcagagg acgttttcca gtccgctttg cgtcagcacg ttcagaatgg gcgctcccgc
      541 cgtgccggag ggctcgccgt catccgtcga gcgggcgacg ccgcccagca ggcgaaacgc
      601 gtaacagaca tgccgtgcgt cccaatgccg ctttctgatc tccgcaatcc gctcggccgc
      661 ttccgcttcc gatgaaacgg ggaaacaata gccaagaaag cgggacttgc tgattacaat
      721 ctcgctttcc ccggctgttt taacagttgt ataaggcgct acgccctgcg tattctgcaa
      781 tagcccttct tgccctttcg aagaataacg ccctcgccgg agatttcttc cggatcaacc
      841 gactcgttga cggactccac tttgcgctcg ccaacgaaca cgccgccgcc ttcgatgagt
      901 cggcgcgcgt cgctcttgct cacggcgagc ttggcaagca ccagaaggtc ggttacgcgt
      961 agtccgctct gcgcaatttc atcggcggaa atctcgtacg tgggaatgtt gctctgatcg
     1021 cttccgcccg caaacagcga ttccgctgcg gtctgcgctt ttttcgcttc ttcctcgccg
     1081 tgcacctgcg cggtgatggt gtaggcaagc gtgcgctttg cttcgttgat ctgttcgccg
     1141 ggcagcgcac ccagacgctt aacttcttcc atgggaatga acgtcagcag cgacagacac
     1201 ttttccacgt cctgatcgcc aacgttgcgc cagtattgat agaaatcgaa cacgctgcac
     1261 ttattggggt cgagccagac ggcgcccttt tcggtcttgc ccatcttctt gccgtcgctc
     1321 gttgtcaaca gcgaagtcgt aagcgcgtat gcgggctttt gctccttgcg gcggatgaga
     1381 tccgcgccgt tgaggatatt gctccattga tcgtccccgc ccatctgcag aatgcagccg
     1441 tagcggcggt tcagttctaa aaagtcgtag ctctgcatga gcatgtagct catttcaaag
     1501 aacgtcagtc ccttttccat acgctgcttg tagcactccg ccgtcagcat gcggttgacg
     1561 gaaaaataca cgccgatatc gcgcatgaag tcgagaaaat tcagcgggta gagccagtcg
     1621 ccgttgtaga cgatctgcgc cttgccctcc gaaaagtcga gcagccgccc catctgcgcc
     1681 ttgaagcaag acgcgttgta agcgagcgtc tctttggaca tcatggtgcg catatccgtt
     1741 ttgccggacg gatcgccgat aaacgccgtg ccgttgccga gc
//
LOCUS       DAOMGY010000400         1772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_77939_length_1772_cov_3.216657, whole genome shotgun sequence.
ACCESSION   DAOMGY010000400 DAOMGY010000000
VERSION     DAOMGY010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77939_length_1772_cov_3.216657"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLR57_06535"
     CDS             <1..332
                     /locus_tag="PLR57_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HQQ41149.1"
                     /translation="AEPAESTEVRMNLSCADCQAYGNGILRDLGAYAAAQGWDVAKEN
                     YEGLRVSFGAGEGDGWFLLRMSLHDPLMPLNIESNSTGGVHAIAQKLYSFLSPYDKLD
                     TSALKNV"
     gene            complement(397..855)
                     /locus_tag="PLR57_06540"
     CDS             complement(397..855)
                     /locus_tag="PLR57_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41150.1"
                     /translation="MFTAEQEKLIQRETERLQGADEAALDALNEDSVLRRTAWFNEHR
                     AGFGFLAGDPLEAGYRLLLARFGITPEQAPVIERTERRIVFHSQNFCPTLEACRRLGL
                     DTRLVCKLLNERATDSLVKLINPRLTFSRNYEQLRPYAPYCDETITLEDE"
     gene            complement(949..>1772)
                     /gene="hutI"
                     /locus_tag="PLR57_06545"
     CDS             complement(949..>1772)
                     /gene="hutI"
                     /locus_tag="PLR57_06545"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HQQ41151.1"
                     /translation="VTATEAKSGYGLDLENELKQLRVLARLKDEAGFDVVPTYMGAHA
                     VPPEFAGKPDDYVEFVCADVLPRVAKEHLAQYCDVFCETGAFSLQQSRRVLETAKKLG
                     LGLKIHADEIDSLGGAELAGELGAVSAEHLIAASDAGISALAKGGVTACLLPGTSLYL
                     NKPFARARDMIERGVAVAVASDFNPGSCPSLNLQLCANLAYLKYRMTPEEILTALTLN
                     AACAIGLGQTHGSIEPGKQADLVLWDADGMELLCYRMGSNQAGIVVKRGKIAHRN"
BASE COUNT          345 a          557 c          504 g          366 t
ORIGIN      
        1 tcgccgaacc ggccgagagc acggaagtgc gcatgaacct ctcctgcgcg gactgtcagg
       61 cgtacggaaa cggcatcctg cgcgacctcg gcgcttacgc cgctgcgcag ggctgggacg
      121 tagccaagga aaactacgaa ggcctgcgtg tttcgttcgg cgcgggcgaa ggcgacggtt
      181 ggttcctctt gcgcatgtcg ctgcacgacc cgctcatgcc gctgaatatc gagagcaact
      241 caaccggcgg cgtgcacgcc attgcgcaga agctctattc gttcctctcg ccctacgaca
      301 agctcgacac gagcgcgctc aagaacgtat aaccggacaa aataaaacgc ttccgtctta
      361 cttggacgga agcgtttttt gttcgcgcgt gtagtgctat tcgtcctcca atgtgatcgt
      421 ctcatcacag tatggcgcat acgggcggag ttgttcgtaa ttgcgcgaaa atgtcagtcg
      481 cgggttaatt agcttgacca gcgagtccgt cgcccgctcg ttgagaagct tacagaccag
      541 ccgcgtatcg aggccgagac ggcggcaggc ttccagcgtc gggcaaaagt tttgggagtg
      601 gaacacgatc ctccgctcag tccgctcgat cacaggcgct tgctccggtg tgatgccgaa
      661 tcgcgccagc agcaggcgat accccgcctc cagcgggtcg ccggcgagaa aaccgaaccc
      721 cgcgcggtgc tcgttgaacc acgccgtcct tcgcaaaacc gagtcctcgt tcagcgcgtc
      781 gagcgccgcc tcgtccgcgc cttgcagacg ttcggtctcc cgttgaatta acttctcttg
      841 ctctgccgta aacatgtgcg attgccctcc ttctgctaaa aatcattgga gcataccacc
      901 gttccaacaa aaaagcaatc ccccgttatt cggaggattg ctgaaatgct aattcctgtg
      961 tgcgatcttt ccgcgcttga ccacgattcc cgcctgattg ctgcccatgc ggtagcagag
     1021 cagttccatg ccgtctgcat cccaaagcac gaggtcggcc tgcttgccgg gctcaatcga
     1081 gccgtgcgtt tggccgaggc ctatggcgca ggcggcgttg agcgtcagcg cagtcagaat
     1141 ttcctccggc gtcatgcggt acttcagata agcgaggttt gcgcagagct ggaggttcag
     1201 tgaagggcac gatcccgggt tgaagtccga ggcaaccgca acggcaacgc cccgttcaat
     1261 catatcccgc gcccgcgcga aaggtttgtt gaggtagagc gatgttcccg gcagcaggca
     1321 agctgtaact ccgccttttg ccagcgcgga aatgcccgcg tcggatgcgg caatcaagtg
     1381 ctccgccgaa accgcaccga gttcccctgc gagttccgcc ccgccgaggg agtcgatctc
     1441 gtcggcgtgg attttcagcc ctaagccgag cttctttgcc gtttcgagca cgcggcggct
     1501 ctgctgaagc gaaaacgcgc ccgtttcgca gaatacgtcg caatactgcg ccaagtgttc
     1561 cttcgccacg cgcggaagca cgtccgcgca gacaaactct acgtaatcgt ccggtttccc
     1621 cgcaaactcc ggcggcacgg cgtgtgcgcc catataggtc ggcacaacgt caaaccccgc
     1681 ttcgtctttg agtcgcgcca gaacacggag ttgtttgagc tcgttctcca aatccagccc
     1741 gtagccgctc ttggcttcgg ttgccgttac ac
//
LOCUS       DAOMGY010000401         1771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_78016_length_1771_cov_3.056527, whole genome shotgun sequence.
ACCESSION   DAOMGY010000401 DAOMGY010000000
VERSION     DAOMGY010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78016_length_1771_cov_3.056527"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLR57_06550"
     CDS             <1..530
                     /locus_tag="PLR57_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41152.1"
                     /translation="TKIIVGVVGLLILNFGFGKIRLLPNTDNVEIDMKFWFGTMSGSP
                     DKLSNQALMVTCMGPLIVATVFWILNRPLNAIAVIYLFIVFWYMLMYITNCQSRWCSE
                     ESPRFSPKERKRKKRADFLFTQIVLLTVPAFLLLLSLLFHWFPIPVWAIIVYSIFGLI
                     YIIGTLATLSQKSGN"
     gene            582..>1771
                     /locus_tag="PLR57_06555"
     CDS             582..>1771
                     /locus_tag="PLR57_06555"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ41153.1"
                     /translation="MQTSELRRDFANCKPEDGYSVGQILAGLYRVECMPIIGGMSMVY
                     RVHHSEWNVDLAMKRPYERLFATEKERELFIAECNAWINLGVHPNVVVCYYVREIEGI
                     PSIFSEWMSAGSLRNWLGKREEDPETGDSKFIPGRLYAGKKKSEIVEQILDIAIQTVR
                     GLFYAQQHDIIHQDVKPDNILLSDDGTAKVADFGISRARHSATSSVSVTATRKNNTYM
                     IEGVGSTKEYCSPEQMEKRWVTKRTDVWSWALVVLEMFLQERLWSDGTVGGYGYDRYR
                     KLAKFEIPPGLDDLLGRCFQNNEADRPHDFSDIEMELLVIYEMTTGREYPRNYLQAVD
                     ETADSLNNHALSNIDIGQLAEAEDFWRRAVRLDHNHLESIYNLDMRLWRRGAIDDIEL
                     LRRL"
BASE COUNT          445 a          438 c          466 g          422 t
ORIGIN      
        1 caacaaaaat catcgtggga gtcgttggct tgctgattct gaattttgga ttcggcaaaa
       61 tcagactgct gccgaatacg gacaatgtgg aaatcgacat gaaattctgg tttggcacga
      121 tgtccggatc accggacaag ttatccaacc aggctctgat ggttacctgt atgggtccgc
      181 tgatcgttgc gacggtattt tggattctga accgcccgtt aaatgcaatt gcggttatct
      241 atctgtttat cgtattctgg tatatgctga tgtatattac gaactgtcag tccagatggt
      301 gctctgagga aagcccgaga ttttcaccaa aagaaagaaa acgtaagaag cgcgccgatt
      361 tcctgtttac gcaaattgta ctgctgaccg ttccggcgtt cctgcttctc ctgtcactgc
      421 tgttccattg gttcccgatt cccgtttggg caattatcgt atacagcata tttggattga
      481 tctacatcat cggtacgctg gctacgcttt cccagaaaag cgggaattaa ttcattatca
      541 tactgttgca tattctccca gcataacgaa agaggttttt catgcagacc agcgaactga
      601 ggcgtgactt tgcaaactgc aagccggaag acggatactc cgtagggcaa atactcgcgg
      661 ggctttaccg cgtggaatgc atgccgatca ttggcggcat gagcatggtc taccgcgtgc
      721 atcacagcga atggaacgtc gatcttgcaa tgaagcgtcc atatgagcgg ctgtttgcca
      781 ccgaaaagga gcgcgaactc tttattgccg aatgcaatgc atggatcaac cttggcgtgc
      841 atcccaatgt tgtcgtatgc tactatgtgc gtgaaatcga gggaatcccc tccatttttt
      901 cggaatggat gagcgcaggc agccttcgaa actggctcgg aaaacgcgag gaagaccccg
      961 aaaccggcga tagcaaattc attcccggaa gattgtacgc cggaaagaag aagagcgaaa
     1021 tcgtcgagca gattctggat atcgccattc aaaccgtgcg cgggctcttt tacgcccagc
     1081 agcatgacat cattcatcag gacgtaaagc cggataatat tctgcttagc gacgacggaa
     1141 cggctaaagt tgcggatttc ggtatctccc gcgcgcgcca ttccgcaacg tccagcgtga
     1201 gcgtcaccgc gacgcgaaag aacaacacgt acatgattga gggcgtcggc tcgaccaagg
     1261 aatattgttc tccggagcag atggagaaac gctgggtcac caaacgcacg gacgtttgga
     1321 gctgggcgct tgttgtgctt gaaatgttcc tgcaggaacg tctctggagc gacggcacgg
     1381 taggcggcta tggttacgac cgctaccgca aactcgcgaa atttgaaatc ccgccgggac
     1441 tggacgattt gctcggaaga tgcttccaaa acaacgaagc cgatcgcccg catgactttt
     1501 cggatatcga gatggagctt ctcgttatct acgaaatgac caccggacgc gaatacccga
     1561 gaaactatct tcaggcggtg gacgaaacgg cggattcgct caacaaccac gcgctttcca
     1621 atatcgacat cggccagctt gcagaagcgg aggatttctg gcgcagggct gttcgactcg
     1681 accataacca tctggaatcg atctacaatt tagacatgcg tctttggcgc agaggtgcaa
     1741 ttgacgacat cgagctgctg cgtcggctca g
//
LOCUS       DAOMGY010000402         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_78427_length_1765_cov_2.785380, whole genome shotgun sequence.
ACCESSION   DAOMGY010000402 DAOMGY010000000
VERSION     DAOMGY010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78427_length_1765_cov_2.785380"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLR57_06560"
     CDS             <1..668
                     /locus_tag="PLR57_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HQQ41154.1"
                     /translation="LDSCGAGLSRRGYRTFNVAAPLAETLGAAIVMLSRYRADLPLID
                     PMCGSGTMPIEAAMIAQNIAPGLNRSFAAESWWFFDKAIWAEERKRAQEQRTNQKIAI
                     SGFDIDPRSIDLCKMHAKKAGVFCDWGVRDIRDFSVGESSGVIVCNPPYGERMLSEKD
                     VKELYRQMRRVFAPYPGWSVNIFSGMREFETVYGKRADKRRKLTNGGMPCTLYQYFGV
                     KDL"
     gene            665..>1765
                     /locus_tag="PLR57_06565"
     CDS             665..>1765
                     /locus_tag="PLR57_06565"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HQQ41155.1"
                     /translation="MKYAVILGDGMADWPVEELGGETPLEAAKHPAMDKLSQAGTFGL
                     VKTIPDGMKPGSDTANLAVFGYDPRKYYTGRSPLEAMSLGINLEQSDVAYRCNLVTLT
                     GGNSLAESVMTDYSAGEISTEEAAELIAFLTERIQDEGVLLYPGFSYRHCLVMKQAET
                     GAELTPPHDISGQSVAGKLPQGTNAELLMRWMERAFQLLGDHPISRARVQAGKNPANA
                     IWFWGEGRRPQLSPFLSKTGLNGAVISAVDLIQGIGKCAGMEVIKVKDATGTFETNFS
                     GKAQAAIDALSRGIDFVYIHLEAPDECGHHGQLKEKIFSIEQIDEKVLTPVWKYLEDS
                     GEDYRILLLPDHPTPLKIRTHSAEPVPFALFRK"
BASE COUNT          412 a          475 c          537 g          341 t
ORIGIN      
        1 gtttggacag ctgcggtgcg gggttatcgc gcagaggcta ccgtaccttt aatgttgcgg
       61 cgccgcttgc ggaaacgttg ggcgctgcga tcgtgatgct ttcacgctac cgcgcggatc
      121 tgccgctgat cgatcccatg tgcggctcag gtaccatgcc catcgaagcc gcaatgatcg
      181 cgcagaacat cgctcccggg ctgaaccgtt cattcgctgc ggagagttgg tggttctttg
      241 acaaagccat ctgggcagag gaacggaaaa gagcacagga gcagaggaca aatcagaaga
      301 tcgcgatttc cggcttcgat atcgacccca gaagcatcga tctttgcaag atgcacgcga
      361 agaaagcggg cgtgttctgc gactggggcg tgcgcgacat ccgcgacttt tcggtaggcg
      421 aatcgtccgg tgtaatcgtc tgcaacccgc cctacgggga gcggatgctg agcgaaaagg
      481 acgtaaaaga gctctaccgg cagatgcgcc gtgtgttcgc gccgtacccc ggatggagcg
      541 tcaatatttt ttccggtatg cgcgaatttg aaaccgtcta cggcaagcgc gcggacaagc
      601 gcagaaagct gacaaacggt ggcatgccat gcacgctgta tcaatacttt ggagtaaagg
      661 atctatgaaa tacgcggtaa tcctgggcga cggcatggcg gactggccgg tggaagaact
      721 gggcggggaa acgcctctcg aagcggcaaa gcatcccgca atggacaaac tttcgcaggc
      781 gggaacgttc gggctcgtta aaacgatacc ggacggcatg aagcccggaa gcgacacggc
      841 aaacctcgcc gtgttcggat acgacccgcg caaatactat acgggccgtt cgccgctgga
      901 agcgatgagc ctcggcatca atctggagca aagcgacgta gcctaccgct gcaacctcgt
      961 tacgcttacc ggcggaaatt cgcttgccga aagcgtaatg accgactaca gcgcgggcga
     1021 aatctccacg gaagaagccg cggagttgat tgcgttcctg accgagcgga ttcaggatga
     1081 aggcgttctg ctctatccgg ggttttcata ccggcattgt ctcgtaatga aacaggcaga
     1141 aacaggcgcg gagctgactc cgccgcatga tatttccggc cagagtgtcg cgggaaaact
     1201 tccacagggg acaaacgcag aactcctgat gcgctggatg gaacgggcgt ttcagctgct
     1261 tggcgatcac ccaatcagcc gcgcgcgtgt gcaggcaggc aaaaacccag caaacgccat
     1321 ttggttctgg ggcgaaggca ggcgccctca gctttcgccg tttctttcaa agacgggtct
     1381 caacggcgcg gttatctccg ccgtggactt gattcagggc atcggcaaat gcgcgggcat
     1441 ggaagtcatc aaggtcaagg acgcaacggg aacgtttgaa acgaatttct ccggcaaggc
     1501 gcaggctgcc atcgacgcgt tgtcccgcgg tatcgatttc gtttatatcc atctcgaagc
     1561 gccggacgaa tgcgggcatc acgggcagct gaaagagaag attttctcga ttgagcagat
     1621 tgacgaaaag gtgctcactc ccgtatggaa gtatctggaa gacagcgggg aagactaccg
     1681 cattctgctg ctgccggatc atcctacgcc gttgaaaatc cgcacccaca gcgcagagcc
     1741 cgtaccgttt gcgctgttta gaaag
//
LOCUS       DAOMGY010000403         1763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_78552_length_1763_cov_3.225410, whole genome shotgun sequence.
ACCESSION   DAOMGY010000403 DAOMGY010000000
VERSION     DAOMGY010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78552_length_1763_cov_3.225410"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLR57_06570"
     CDS             complement(<1..500)
                     /locus_tag="PLR57_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HQQ41156.1"
                     /translation="MRDVYESPLNSRYASAEMQRLFSPDMRYSTWRRLWTSLARAEHA
                     LGLPVTQQQVDELAAHQTDIDYDAVAAREKQVRHDVMAHVYAYGLVCPNAAGIIHLGA
                     TSCFVTDNADLIILRDALLSIQNSVCAVLNALKPFAERTKAVPTVGYTHFQAAQPVTI
                     GRSRLS"
     gene            complement(504..>1763)
                     /locus_tag="PLR57_06575"
     CDS             complement(504..>1763)
                     /locus_tag="PLR57_06575"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQQ41157.1"
                     /translation="AIVGINWGDEGKGRMVDLIAEDYGVVVRYQGGNNAGHTVVNEYG
                     KFALHLLPSGIFRKGVVNVLGNGAVVDPEKLLEEIEDLRSKGISVTPENLKVSDRAPI
                     VFPYHRDQDALEEARLQDAKYGSTKRGIAPVYGDKYMKKTIMLGELFYPDYLKKRLEG
                     ILEWKNLILKNVYGAKTYSLNELMDWLIAYGEPLKPFICDTGAFLREADGKKNILFEA
                     QLGVLRDLDFGIYPYTSSSAPLAAYAPIGAGMPGAKIDRVIGVVKAYSTCVGEGPFTA
                     EWFGEEASRLREAGSEYGAATGRPRRVGPMDIVATRYGVAMQGATEVALTKIDILSYM
                     ENIPVCVQYELHGKKTDDFPFPAVVGDAKPVIEYLPGWGTDISGCRSFDELPKAAQDY
                     ILYIEKNIRCPITNVSVGADRDAIIRR"
BASE COUNT          326 a          502 c          558 g          377 t
ORIGIN      
        1 aaggacaacc tgctgcggcc gatggtcacc ggctgtgcag cctgaaaatg cgtgtagccg
       61 acggtcggaa ccgctttggt gcgctcggca aagggcttga gcgcgttgag cacggcgcag
      121 acactgtttt gaatgcttag aagcgcgtcc cgcaggatga tcaaatccgc gttgtcggtt
      181 acaaagcagc tggtcgcacc aagatggatg atgcccgccg cgttggggca gacgagcccg
      241 taggcataga catgcgccat cacgtcgtgg cgcacctgtt tttcgcgtgc cgccaccgcg
      301 tcgtaatcga tatccgtctg atgcgcggca agttcgtcca cctgttgttg ggtaaccggc
      361 aagccaaggg cgtgctccgc gcgcgcaagg ctcgtccaga gcctgcgcca ggtggaatac
      421 cgcatatccg gcgagaagag ccgctgcatt tcggcagagg cataccggct gttgagcggg
      481 ctttcgtaga cgtcgcgcat ggactacctc cggatgatcg cgtcgcggtc cgcgccgacg
      541 gagacattcg tgatcgggca gcggatgttc ttttcgatat agagaatgta atcctgcgct
      601 gccttgggca gttcgtcaaa cgagcggcag ccggagatat cggttcccca gccgggcagg
      661 tattcgatga ccggcttggc gtcgcctacg accgcgggga aggggaagtc gtccgtcttt
      721 ttgccgtgga gctcgtactg cacgcagacg gggatatttt ccatatagga aaggatgtcg
      781 atcttggtga gcgccacttc cgttgcgccc tgcatggcta cgccgtagcg tgtggcaacg
      841 atgtccatgg ggccgacccg gcggggacga ccggtcgccg caccgtattc agaacctgcc
      901 tcgcgcagac gcgaagcttc ctcaccaaac cattccgccg tgaagggacc ttcacccacg
      961 caggtggagt atgctttgac aacgccgatc acgcggtcga tctttgcgcc cggcatgccc
     1021 gcgccgatgg gcgcgtaggc cgcaagagga gcggagctgg acgtgtaggg atagatgccg
     1081 aaatccaggt cgcggagcac gccaagctgc gcttcgaaga ggatgttttt cttgccgtcc
     1141 gcttcccgca ggaacgcgcc cgtgtcgcag atgaagggct tgagcggttc gccgtaagca
     1201 atgagccaat ccatgagctc gttgagcgag taggtttttg cgccatagac gtttttgagg
     1261 atcagattct tccattcgag gatgccttcg agccgctttt tgaggtaatc gggatagaac
     1321 agctcgccga gcatgatggt ctttttcatg tacttatcgc cgtagacggg cgcgatgccg
     1381 cgcttggtgg agccgtattt ggcgtcctgc aggcgcgctt cttccagcgc gtcctgatcg
     1441 cgatggtagg gaaagacgat gggcgcgcgg tcgctgacct tgaggttttc cggcgtgacc
     1501 gaaatgccct tgctgcgaag gtcttcaatc tcctcgagca gtttttccgg atcgaccacc
     1561 gcgccgttgc cgagcacgtt gactacgcct ttgcggaaaa tgccggaagg aagcaggtgc
     1621 agcgcgaatt tgccgtattc gttgacgacg gtgtgacccg cgttgtttcc gccctggtag
     1681 cggacgacca cgccgtaatc ttccgcaatg agatccacca tgcggccttt gccttcgtcg
     1741 ccccagttga ttcctacgat tgc
//
LOCUS       DAOMGY010000404         1754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_79158_length_1754_cov_3.093584, whole genome shotgun sequence.
ACCESSION   DAOMGY010000404 DAOMGY010000000
VERSION     DAOMGY010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79158_length_1754_cov_3.093584"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="PLR57_06580"
     CDS             complement(<1..1299)
                     /locus_tag="PLR57_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41158.1"
                     /translation="MKEIGFVCFGEVNTPYERLVLMHDAAVETLSALGGNLLDAGLVI
                     DDVKYETADAALGKLKTRELDCLIVCVAGWVPTHAVIRVTDAFRHLPVLLWGLCGWKE
                     NGKIVTTAAQAGTTAIRPALEAMHYRFKYVYSVIGKPEPIEKIDAFVSAAHAAKLLRS
                     ARVGSMGYRDMLLYGTQYEGNSMRGQLGVEVEPFEMLEVVQNIEQLDPEEIAENIAFI
                     KANWVFDKTCDDGVIETGVKYALAVGKKIKERGYKAVTLIDVDGMKKLLGFPPAMVFM
                     LLERNYGVLTVPENDVMGAVTQLIANGLTGDIVPYLEYYEFFEKSMLIGVPDYVPAAA
                     TEGQTHVLPTAFGLLSASLLNVSRVKTGLVTCLRLINKDGRYGMHMYLADAKNPPDWE
                     EFGWAPPAPHLPSLEVFPRDCTVEEFSQKVSSQHVIVCYGD"
     gene            complement(1355..>1754)
                     /locus_tag="PLR57_06585"
     CDS             complement(1355..>1754)
                     /locus_tag="PLR57_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQQ41159.1"
                     /translation="IRESHVKRGKERFELGGYLRAKELIRETEEPRVTAVVACYDQVA
                     LGALNAFAEAGIRVPEDLSVTGFDNIILNDYLPIQLTSISNPTEQLSALAVKLLMDNL
                     HERENHVVQNVSLQPRLVIRSSTAAPRSHM"
BASE COUNT          325 a          551 c          486 g          392 t
ORIGIN      
        1 atcgccgtag cagacgatca cgtgctggct ggagaccttc tgagaaaact cttccaccgt
       61 gcagtcgcgc gggaaaactt ccagactcgg cagatgcggc gcgggcggcg cccagccgaa
      121 ctcttcccaa tcgggcgggt tcttcgcgtc cgcaagatac atgtgcatgc cgtaacggcc
      181 gtccttatta atcagccgca ggcaggtgac cagccccgtt tttacgcggg aaacgttgag
      241 caagctggcc gagagcagcc cgaacgcggt cggcagaacg tgtgtctgcc cctccgtcgc
      301 ggcggcgggc acatagtccg gcacgccgat gagcatgctc ttttcaaaaa actcgtagta
      361 ttccagatac gggacgatat cgcccgttaa tccgttggca atcagctgcg tcaccgcgcc
      421 catcacgtcg ttttccggca cggttaatac gccgtaattt cgttccagca gcataaacac
      481 catcgcgggc ggaaaaccga gcagcttctt catgccgtca acgtcaatca gcgtgaccgc
      541 tttatagccg cgctccttaa tcttcttgcc gaccgccagc gcgtatttta cgccggtttc
      601 gattacgccg tcgtcgcagg ttttgtcaaa cacccagttt gccttgatga acgcaatgtt
      661 ctccgcaatc tcctccgggt cgagctgctc aatattctgc acgacttcca gcatttcgaa
      721 cggctcgact tccacgccga gctgcccgcg catgctgttg ccttcgtact gcgtgccgta
      781 gagcagcatg tcccggtagc ccatggagcc gacgcgcgcg ctcctgagga gctttgccgc
      841 gtgcgccgcg ctcacaaacg cgtcgatttt ctcgataggt tcgggctttc cgatcacgct
      901 gtaaacgtat ttgaaccggt aatgcatggc ttccagcgcc ggacggatgg ccgtcgttcc
      961 cgcctgtgcc gccgtggtga cgatcttgcc gttctctttc cagccgcaga gcccccagag
     1021 gagcaccggc aggtggcgga atgcgtccgt cacgcggatg accgcgtgcg tcggcaccca
     1081 gcccgcaacg cagacgatca ggcagtccag ctctcgcgtt ttaagtttac cgagcgcggc
     1141 gtcggcggtt tcgtacttca cgtcgtctat cacaagaccc gcgtccagca ggtttccgcc
     1201 gagcgcactg agcgtttcca ccgctgcgtc gtgcatgagc acgagacgct catacggcgt
     1261 attcacttcg ccaaagcata caaaaccgat ttccttcatt gttctctctc cttttgctta
     1321 ctgggaattc acaaggcgcg ttttacgctc cgcattacat atggctgcgc ggtgcggcgg
     1381 tcgagctccg tatcaccagc ctcggctgga gggaaacatt ctgcaccacg tggttttccc
     1441 gttcatgaag gttgtccatc agcagtttga ccgcgagcgc actgagctgt tcggtcgggt
     1501 tggagatgga agtcagctga atcggcaggt agtcgttgag tatgatgttg tcgaacccgg
     1561 tcaccgaaag gtcttccgga acgcggatgc ccgcttcggc aaacgcgttg agagcgccca
     1621 gcgcaacctg atcgtaacag gcgactaccg cggtcacgcg cggttcctcc gtttcgcgaa
     1681 tcagctcttt tgccctgagg tacccgccca gctcaaagcg ttccttgccg cgtttgacgt
     1741 ggctttcccg gatg
//
LOCUS       DAOMGY010000405         1750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_79439_length_1750_cov_2.919174, whole genome shotgun sequence.
ACCESSION   DAOMGY010000405 DAOMGY010000000
VERSION     DAOMGY010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79439_length_1750_cov_2.919174"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PLR57_06590"
     CDS             complement(<1..824)
                     /locus_tag="PLR57_06590"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HQQ41160.1"
                     /translation="MKKRTVRLLAALLVVICCLMLGAYSLADAGNFSGDTDWGGGGSD
                     WGSSGSDWGSSSSSSDWGSGSTYYSDGSSDYSGSGGCLGGNLFTIAIVVIVIIVMLRY
                     RKSGKGGSGSQVYQAAQETPGLPIETLKQKDPNFNEQAFLEKIGNWYIQMQNAWEAKK
                     WEPMRAIMTDALYTQMERQLQSLVQAGRTNHVERIAVLDSRIVRYAVEGDNDVLVVRI
                     STRICDYTTDDRTGQVVSGSQTRELFMTYDWKLIRQKDQITPEQEGMTHINCANCGA"
     gene            complement(988..1233)
                     /locus_tag="PLR57_06595"
     CDS             complement(988..1233)
                     /locus_tag="PLR57_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HQQ41161.1"
                     /translation="MSEYIATFHTHLAAMRSQRTLKGGGISARLSPVPRALSASCGTC
                     VRYEAEDPCVSAMDRDLERIVKCVPDGETYEPVLEKD"
     gene            complement(1230..1439)
                     /locus_tag="PLR57_06600"
     CDS             complement(1230..1439)
                     /locus_tag="PLR57_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003148659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HQQ41162.1"
                     /translation="MDIIDARGLLCPEPVVLAQKAVKSEPAEVAVLVDAVAAKENVTR
                     FAVSRGYEVSVSEQKGEYTLTLKKK"
     gene            complement(1477..>1750)
                     /locus_tag="PLR57_06605"
     CDS             complement(1477..>1750)
                     /locus_tag="PLR57_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YedE-related selenium metabolism membrane
                     protein"
                     /protein_id="HQQ41163.1"
                     /translation="TDGLWNFLGMALVGWGSVLLGGCPLRQVILAGEGNSDAGITVTG
                     FFVGAAICHNFGLASSGKGPTVNGMIAVAAGFVLLTIIGLLNREKA"
BASE COUNT          340 a          549 c          479 g          382 t
ORIGIN      
        1 gcgccgcagt ttgcgcagtt gatatgcgtc atgccctcct gctccggcgt aatctgatcc
       61 ttctggcgaa tcagcttcca gtcgtaggtc atgaacagtt cacgcgtctg actgccgctg
      121 acgacctgac ccgttctgtc atcggtcgtg tagtcgcaga tgcgcgtcga gatccgaacg
      181 accagcacgt cgttgtcgcc ttccacggcg tatctcacga tgcgggagtc gagcaccgcg
      241 atgcgctcaa catggttggt gcgccccgcc tgcacaagcg actggagctg gcgctccatc
      301 tgcgtgtaga gcgcgtcggt catgattgcg cgcatgggct cccacttctt ggcttcccag
      361 gcgttctgca tctgaatgta ccagttgccg atcttttcga ggaacgcctg ttcgttgaag
      421 ttcgggtcct tctgcttgag cgtctcaatc ggcagacccg gcgtttcctg cgccgcctga
      481 tagacctgcg aaccgctccc gcccttgccg gacttacggt aacgcagcat gacgattatt
      541 acgatgacga caatcgcaat cgtaaaaagg ttgccgccca ggcatccgcc ggaaccggag
      601 taatcgctcg aaccgtcgct gtaataagtc gatccgcttc cccaatccga ggaagagctc
      661 gaactgcccc agtcggagcc ggagctgccc cagtcggaac cgccgccgcc ccagtcggta
      721 tcgccggaaa agttgcccgc gtcggcaagc gaatacgcgc ccagcatcag acagcaaatc
      781 acgaccaaca gcgccgcaag cagccgcacc gttcgttttt tcatacgcct tcaacccccc
      841 aaatgatttt tacgcaatta tcataccgta ttttgggttc ggtaacaata cagaaccact
      901 gcaggaatta gcgcctatta tatgggcgcg catgaaaaaa cggcccccgc cgtcaggcaa
      961 taggccgttt tgtttgttca gcgcgctcta gtccttttcc agcaccggtt catacgtttc
     1021 gccgtccggc acacatttga cgatgcgctc caaatcgcga tccattgcgg aaacgcaggg
     1081 gtcttccgct tcgtagcgaa cgcaggtgcc gcagcttgcg ctgagcgcgc gcggcacggg
     1141 cgacaggcgc gcgctgattc cgccgccttt gagcgtcctc tgggagcgca tggccgcaag
     1201 gtgcgtatga aacgttgcaa tgtactcgct catttctttt tgagcgtcag cgtgtattcg
     1261 cctttttgtt cgctgacgga aacttcgtat ccgcgcgaaa cggcaaagcg ggtaacgttt
     1321 tcctttgcgg caacggcgtc caccagcacg gcgacttccg cgggttcgga cttgaccgct
     1381 ttctgcgcca gtacgacggg ttccgggcag agcagccctc ttgcgtcgat aatatccatt
     1441 tcgttttcct ttccgtactt ccgtattgtt tcgcgtttac gccttttcgc ggttgagaag
     1501 gccgatgatt gtgagcagca caaaacccgc cgccaccgcg atcatgccgt tgacggtcgg
     1561 ccctttgccc gaagaagcaa ggccgaagtt atggcagatt gccgcgccaa caaagaagcc
     1621 ggtaaccgtg atacccgcgt cggaattgcc ttcgcctgcg aggataacct gccgaagcgg
     1681 gcagccgccg agcagaacgc tgccccagcc gaccagcgcc atgccgagga agttccagag
     1741 cccgtccgta
//
LOCUS       DAOMGY010000406         1749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_79497_length_1749_cov_3.612161, whole genome shotgun sequence.
ACCESSION   DAOMGY010000406 DAOMGY010000000
VERSION     DAOMGY010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79497_length_1749_cov_3.612161"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PLR57_06610"
     CDS             complement(<1..591)
                     /locus_tag="PLR57_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HQQ41164.1"
                     /translation="MAKTMTIDGNTAASHVAYAFSDVAAIYPITPSSPMAEVADDWAA
                     NGRTNLFGQTVRIAEMQSEGGAAGAVHGSLKGGALTTTFTASQGLLLMIPNMYKISGE
                     LLPGVFHVSARALAAHALSIFGDHSDVMSCRQTGFAMLASSSVQEVMDLGLVTHLAAI
                     KSSVPFLHFFDGFRTSHEVQKIEMIEYEDMGKLLDYD"
     gene            complement(861..1346)
                     /locus_tag="PLR57_06615"
     CDS             complement(861..1346)
                     /locus_tag="PLR57_06615"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HQQ41165.1"
                     /translation="MKNLTIRELVRGAIIAAIYALLTIFLAPISSGLIQCRVAEALCV
                     LPYFTFSAVPGLFIGCLAANLLTGAPIWDVLFGSLATLMAAYATYLLRNRASKYLAPL
                     PSVVFNALIVGALLVYVYAVPVTYPVAAAYVAAGQIVACFALGLPLMAVLERFRGKLF
                     D"
     gene            complement(1398..>1749)
                     /locus_tag="PLR57_06620"
     CDS             complement(1398..>1749)
                     /locus_tag="PLR57_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ41166.1"
                     /translation="AYLMHRLQWGKSAPDGYDLLKLATRGGAKLLGREELGQIATGCA
                     ADAIAIRYDRAELVCAEQDEANLFGTVGYHRPADYVWVNGRLTVRDGAVLTLDEDKLA
                     VESRDEVKRLLEHA"
BASE COUNT          396 a          500 c          485 g          368 t
ORIGIN      
        1 gtcgtagtcg aggagcttgc ccatatcctc gtattcgatc atttcgatct tctgcacttc
       61 gtgggaggtg cggaagccgt caaagaagtg caggaacgga acgctgctct tgatggcggc
      121 aagatgcgtc acaaggccga ggtccataac ttcctgaacg gaagaagagg ccagcatggc
      181 aaaaccggtc tggcggcagc tcatgacatc cgaatggtcg ccaaagatcg acagcgcgtg
      241 ggcggcaagc gcgcgggcgc ttacatggaa cacgccgggg agcagttcgc cggaaatctt
      301 gtacatgttc gggatcatca gcagcagacc ctgagaagcc gtgaacgtag tcgtcagcgc
      361 gccgcccttg agagagccgt gcacggcgcc ggcggcgccg ccttcgctct gcatttcagc
      421 gatgcgtaca gtctgtccaa acaagttcgt tctgccgttg gcagcccaat cgtccgcaac
      481 ttccgccatg ggagaggaag gcgtgatcgg gtaaattgcc gctacgtccg aaaacgcata
      541 cgcaacgtgc gacgcggcgg tgttcccgtc gatggtcatg gtttttgcca ttttgtggat
      601 gcctcctaag tgatatgtat ggaatcgagt tttcgtttat gaaaaaccct atgattaccg
      661 caaatattat atctttaagc aagtcaactg tcaatcaaag cggcaacgta tttgcaggaa
      721 ttccgccgaa tatttacgta ttatctctgt ttgtttcata tttctgcaag aaaccttgca
      781 ggccgagaaa tttcactttt cgctgttcac atgaaaaaaa agcgaggtct cccccgcttt
      841 tttgagccgt tttatgctga tcagtcgaac agcttgccgc ggaagcgttc gagcaccgcc
      901 atgagcggca gaccgagcgc aaagcaggca acgatctgcc ccgccgcgac atacgccgcc
      961 gcaacgggat acgtaaccgg caccgcgtaa acatacacca acagcgcgcc tacgatgagc
     1021 gcattgaaca caaccgaagg cagcggagcc aggtatttgc tggcgcggtt tcgcaaaaga
     1081 tacgttgcat acgccgccat cagcgttgcc agactgccaa agagcacatc ccaaatcggc
     1141 gcgccggtta aaaggtttgc cgccaggcac cctataaata agcccggcac ggcggaaaac
     1201 gtaaaatacg gtaaaacgca gagtgcttcc gccacacggc actgaatcag gccgctggaa
     1261 attggagcta agaagattgt caacagcgcg tagatcgccg cgataatcgc cccgcgcacg
     1321 agttcccgaa tggtgagatt tttcatatgc tagctgcgag cctccgtgga ctttgccgcc
     1381 ctttcgattg attgatttta cgcatgctcc agcaggcgtt tgacttcgtc gcgcgattcc
     1441 acagctagtt tgtcctcgtc cagcgtcagc accgcgccgt cacggacggt cagcctgccg
     1501 ttgacccaga cgtagtccgc cgggcggtgg tagccaaccg ttccaaatag attcgcctcg
     1561 tcctgttccg cgcagacgag ttccgcgcgg tcgtaacgga tggcaatcgc gtccgccgcg
     1621 cagccggttg cgatttgacc gagctcttcg cgccccagca gttttgcgcc gccgcgcgtg
     1681 gcaagtttga gcaggtcgta gccatccggc gcggatttac cccactgcag ccggtgcatg
     1741 agatacgcc
//
LOCUS       DAOMGY010000407         1743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_79944_length_1743_cov_2.770735, whole genome shotgun sequence.
ACCESSION   DAOMGY010000407 DAOMGY010000000
VERSION     DAOMGY010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79944_length_1743_cov_2.770735"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="PLR57_06625"
     CDS             <1..160
                     /locus_tag="PLR57_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005360913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HQQ41167.1"
                     /translation="AVAELILLFVTLKHILTHKTYKRGSRTLWLIVVIVGMEFIGPIL
                     YFLLGKED"
     gene            166..1071
                     /locus_tag="PLR57_06630"
     CDS             166..1071
                     /locus_tag="PLR57_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ41168.1"
                     /translation="MNILEVNRVDKRFGSREVLKGLSFSVPEHTIYELIGANGAGKTT
                     TMKLILGLLPLDGGEIRVCGEQVRYGQTKTNRHIGYLPDVPEFYGYLTPVEYLTLSGS
                     IAGMHPDELKARTAELLSLVGLEQSAARRINGFSRGMKQRLGIAQALIHRPRLLVCDE
                     PTSALDPAGRKDVIDVLKAIKHQTTVLFSTHILSDVERICDEIGLLHGGSMRLEGKLE
                     EIRQMHAGSGYAIEFQDEADASRFLAAHPGGELASSTRLLFASGTQEEMSRAMGALAS
                     MGICPVKIELCEPSLESLFLEAAGL"
     gene            1068..>1743
                     /locus_tag="PLR57_06635"
     CDS             1068..>1743
                     /locus_tag="PLR57_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQQ41169.1"
                     /translation="MKQIVTLLKKESTELFRTGKATVLLIVFVIFGVMNPALAKLTPW
                     MLNLMSSSLSDAGITIGNITVDAMTAWTQYFKNLFMEYILVLALFCGTLTNECQSGTL
                     VNLLAKGLPRWKVVAVKFLSAFAVWSVCYWLTFAVTFGYSAYFWDNSIVMHLGLASFS
                     AYLLGVWLLALILMFSAFLRSGMYVLLATGAVYGVSAALGALPMLTKYLPARLDGAFG
                     LLTGVLV"
BASE COUNT          331 a          544 c          504 g          364 t
ORIGIN      
        1 tgcggttgcg gaactcatcc tcttgttcgt aacgctcaag cacatcctga cgcacaaaac
       61 ctacaagcgc gggtcgcgca cgctttggct catcgtcgtc atcgtcggta tggaattcat
      121 cggccccatc ctctacttcc tgctcggcaa agaggactaa cgcaaatgaa cattctggaa
      181 gtaaaccgcg tcgataagcg cttcggcagc cgggaagtgc tcaaaggcct ttccttcagc
      241 gtgccggaac atacgattta cgagttaatc ggcgcaaacg gcgcgggcaa aacgacgacc
      301 atgaagctca tcctcggcct gctcccgctg gacggcggcg aaatccgcgt ctgcggcgag
      361 caggttcgct acgggcagac caaaaccaac cgccacatcg gttacctgcc ggacgtgccg
      421 gaattctacg gctacctgac cccggttgaa tacctgacgc tcagcggcag catcgccggg
      481 atgcacccgg acgagttgaa agcgcgcacc gccgaactcc tctccctcgt cggtctggag
      541 caaagcgcag cccgccgcat caacggcttt tcgcgcggca tgaagcagcg cctcggtata
      601 gcgcaggcgc tcatccaccg tccgcggctg ctggtctgcg acgagccgac ctccgcgctc
      661 gacccggcgg ggcgcaagga cgttatcgac gtgctcaagg cgatcaagca ccaaaccaca
      721 gtgctgtttt ccacgcatat cctctcggat gtcgagcgaa tctgcgacga aatcggcctg
      781 ctgcacggcg gttccatgcg tttggaaggc aagctcgagg agattcgcca gatgcacgcg
      841 ggcagcggct acgccattga gtttcaggac gaagcggacg cttcccgctt tcttgccgcg
      901 caccccggcg gcgaactcgc ctcctccacg cggctgctgt ttgccagcgg aacgcaggag
      961 gagatgtccc gcgcgatggg cgcgcttgca agcatgggca tctgccctgt taagatcgag
     1021 ctttgcgaac cttcgctgga gtctctgttt ctggaggcgg caggcttatg aaacagattg
     1081 ttacgctact aaaaaaggaa tccacagagc tgttccgcac cggaaaagcc acggtgctgc
     1141 tgatcgtgtt cgtcatcttt ggcgtgatga accccgcgct ggcaaagctc acgccctgga
     1201 tgctgaattt gatgtccagc tccctctccg acgcgggcat tacgatcggc aatatcacag
     1261 tggacgcgat gacggcctgg acgcagtact ttaagaacct ctttatggag tatatcctcg
     1321 tgctcgcgct cttctgcgga acgctgacaa acgagtgcca aagcggcacg ctcgtcaacc
     1381 ttctggcaaa aggtctgccg cgctggaagg tcgtagcggt caaattcctc tcggcgttcg
     1441 ccgtctggag cgtttgctac tggctgacat ttgcagtcac gttcggctac agcgcgtact
     1501 tctgggacaa ctccatcgtg atgcatctgg ggcttgcgtc gttcagcgca tacctgctgg
     1561 gtgtgtggct gctcgcgctg atactgatgt tctctgcgtt tctccgctcc ggtatgtacg
     1621 ttctgctggc caccggcgcg gtctacggcg tttccgccgc gctcggcgcg ctgcctatgc
     1681 tgacaaagta tctgcccgcg cggctcgacg gcgcgttcgg cctgctcaca ggcgtgctgg
     1741 ttc
//
LOCUS       DAOMGY010000408         1739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_80253_length_1739_cov_2.379454, whole genome shotgun sequence.
ACCESSION   DAOMGY010000408 DAOMGY010000000
VERSION     DAOMGY010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80253_length_1739_cov_2.379454"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLR57_06640"
     CDS             complement(<1..214)
                     /locus_tag="PLR57_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HQQ41170.1"
                     /translation="MAKMYYEGDCNLGFLNNKTVAIIGYGSQGHAHALNLKESGVNVV
                     VGLYEGSKSWKKAEEAGLRVATAKDAA"
     gene            complement(387..896)
                     /gene="ilvN"
                     /locus_tag="PLR57_06645"
     CDS             complement(387..896)
                     /gene="ilvN"
                     /locus_tag="PLR57_06645"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HQQ41171.1"
                     /translation="MKLTMSVLVKNHAGILYKVAGLFSRRAYNIHSLAVGTTSDPRVS
                     RMTIVVDGDEEIFEQVEKQLCKLIDVIQVKILVPGEFINRELVLIKVNANSETRSDIL
                     QIAEIFGAKSVDVSHETLILECCGKSEHIQRFETMLERYGVCETVRTGMIAIENAHVM
                     APADGQKSH"
     gene            complement(931..>1739)
                     /gene="leuB"
                     /locus_tag="PLR57_06650"
     CDS             complement(931..>1739)
                     /gene="leuB"
                     /locus_tag="PLR57_06650"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HQQ41172.1"
                     /translation="VAGIRAALGLFANLRPAVLFDSLADACPLKPEVMATGLDLLMVR
                     ELTGGIYFGQRGRSAGANGAEAFDTERYSELEIERIGRVGFESAMKRRKKITSVDKAN
                     VLESSRLWRETMHRLAKEYPDVQYEDMLVDNAAMQLVRNPGHFDVIVTSNLFGDILSD
                     EASMITGSIGMLPSASLGSGTLGLFEPIHGSAPDIAGKGIANPLATILSAAMLLRYSL
                     CQPEAADAVEQAVQAVLRQGLRTADIAAGKPSLGTVEMGDAVCRALTQAG"
BASE COUNT          301 a          530 c          501 g          407 t
ORIGIN      
        1 tggcggcgtc cttcgccgtt gcaactctta agcctgcttc ttccgctttt ttccaggatt
       61 tgctgccttc gtaaagcccg accacgacgt tcacgccgct ttccttaagg ttcagcgcat
      121 gcgcgtgccc ctgactgccg tagccgatga ttgcaacggt tttgttgttc agaaacccca
      181 gattgcagtc gccctcataa tacatctttg ccataataaa ccctttcctg cgcgtaagcg
      241 ccttttttat tttttgaccg gctgtatgcc ggatgttctg ctatttggtt tggttatcta
      301 gtgaatccgc aagcgccatg acgtgcgcat tttcgatcaa tggcttttct gcccgtccgc
      361 gggcgccatg acgtgcgcat ttctgatcaa tggctttttt gcccgtccgc gggcgccatg
      421 acgtgcgcat tttcgatggc gatcatgccg gttcgcaccg tttcgcagac gccgtagcgc
      481 tccagcatgg tctcgaaccg ctggatgtgc tcgcttttgc cgcagcactc caaaatgagc
      541 gtctcgtggg aaacgtccac cgacttcgcg ccgaaaatct ccgcaatctg gaggatatcc
      601 gaacgggttt ccgagtttgc gttgaccttg ataagcacca gttcgcggtt gataaactcg
      661 cccggcacga ggattttcac ctgaatgacg tctatgagct tgcagagctg tttttccacc
      721 tgctcaaaga tctcctcgtc gccgtccaca acgatggtca tgcgcgaaac gcgcgggtcg
      781 gacgttgtgc ccacggcgag gctgtggatg ttgtaggccc ggcgggagaa cagccccgcc
      841 accttataca ggattccggc gtggtttttc accagcacgg acatggtcag tttcataatc
      901 gctcctttct gaatggttcc cgcatctccg ttacccggcc tgcgtcagcg cgcggcatac
      961 ggcgtcgccc atttcgacgg ttccgagcga gggtttgcct gctgcgatat ccgccgtgcg
     1021 caagccttgc cggagcaccg cctgtacggc ctgttccacc gcatccgccg cttcgggctg
     1081 acagagcgag tagcgcagca gcatcgccgc cgagaggatc gttgccagcg ggtttgctat
     1141 gcctttgccc gcaatatccg gcgcggagcc gtggatgggc tcgaacaggc cgagcgttcc
     1201 gctgccgagg gacgcggaag gcagcatgcc aatggagccg gtgatcatgc tcgcctcgtc
     1261 ggagaggata tcgccaaaga ggttgctggt aacgattacg tcgaagtgcc cggggtttcg
     1321 caccagctgc atggccgcgt tgtccacgag catatcctcg tactgaacgt ccgggtattc
     1381 ctttgccaga cggtgcatgg tttcgcgcca gaggcgggag ctttccagca cgttcgcctt
     1441 gtccacgctg gtgatctttt tgcgccgctt cattgcggac tcgaacccga cgcggccgat
     1501 gcgctcgatt tccagctcgg aataccgctc ggtatcgaag gcttccgcgc cgtttgcgcc
     1561 cgcgcttctg ccgcgctggc caaagtagat gccgccggtg agctcgcgca ccatcaaaag
     1621 gtcgagaccg gtcgccatga cttccggttt gagcgggcag gcgtcggcta aggaatcgaa
     1681 cagtacggcg gggcgcaggt tggcaaacaa ccccagcgcg gcgcggatgc cagccacag
//
LOCUS       DAOMGY010000409         1733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_80659_length_1733_cov_2.525030, whole genome shotgun sequence.
ACCESSION   DAOMGY010000409 DAOMGY010000000
VERSION     DAOMGY010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80659_length_1733_cov_2.525030"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLR57_06655"
     CDS             <1..296
                     /locus_tag="PLR57_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41173.1"
                     /translation="LLAQIRPVIESPVLQPAFDNILPALFGALAVVFVSRNWKIAIAP
                     MVFMIALFVLVPSLASKVGILVPVGALIAIGVGRILYKNGLLAEKPKHAKTAE"
     gene            443..>1733
                     /locus_tag="PLR57_06660"
     CDS             443..>1733
                     /locus_tag="PLR57_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQQ41174.1"
                     /translation="MTYVWLALAALVVLFFAVVLIRALLFKPKAGKQGAPTEAVVDEQ
                     KAIDDLAEMIRCKTVSSYDESKIDWSEFKKFRTLLKKLYPTVFKHCGYEEIGKTGVLF
                     TLKGKSAEKPAVFMAHYDVVPVNEEGWAKPAFDGIIEDGVLWGRGTLDTKCTLLGVLE
                     SAELLLKQGFVPENDMYFAFAGDEEVAGKAQPEIVETLRSRGVVPAIVVDEGGAVVEG
                     IFPGVKAPCALIGIAEKGLMDAQFVIEGAGGHASAPPPHTGLGRIARAVCSVENKPFP
                     RVLTKPISEMFDTLGRSSTFLYRVIFANLWCFLPLLDALCKKTGGELNAMMRTTCAFT
                     MAEGSKTSNVLPPRVSVVANLRLISGTTREQTLDMLRERVEDKDIKVSMLHGMDPCKC
                     SETEGYGWEKLKSAIAETWPDALISPYLMIAASDSRHY"
BASE COUNT          350 a          503 c          527 g          353 t
ORIGIN      
        1 tgctgctggc gcagatccgg cccgtcatcg aaagccccgt tctgcagccc gcgttcgaca
       61 acattctgcc cgcgctgttt ggcgcactcg cggtggtgtt cgtttcccgc aactggaaaa
      121 tcgccatcgc tccgatggtg ttcatgatcg cgctgttcgt tcttgtgcct tcgcttgcca
      181 gcaaagttgg cattctcgtg ccggtcggcg cgctgatcgc catcggtgtt ggccgcatcc
      241 tctacaagaa cggcctgctc gccgaaaagc cgaagcacgc caaaaccgcc gaataaacaa
      301 ggcaagtaac gcggcgcgtg aaaaaacgcg tatttacgga ttattttacc gcgccgtttt
      361 cggcttacag tagtatactg tacgggaaag caaatctcga gctcctacct caacggggag
      421 cgggacagga ggaatcgtat ccatgacgta tgtttggctg gcactggccg cgctggtcgt
      481 gttgtttttc gcggtcgtgc tgattcgggc gctgctgttc aagcccaaag cgggcaaaca
      541 gggcgcgccg accgaagcgg tcgttgacga gcagaaggcg attgacgacc tcgccgagat
      601 gattcgctgc aagacggttt cgtcttacga cgagagtaag atcgactggt cggagttcaa
      661 aaagttccgt acgctgctca aaaagctgta tcccaccgtg ttcaagcact gcgggtacga
      721 agagatcggc aagaccggcg tgctctttac cttaaagggc aagagcgccg aaaagcccgc
      781 cgtgttcatg gcgcactacg acgtggtgcc cgtcaacgaa gaaggctggg caaagcccgc
      841 gttcgacggc atcattgagg atggcgttct ctgggggcgc ggcacgctgg ataccaagtg
      901 cacgctttta ggtgtgctgg aatccgccga actgctctta aagcagggat tcgtgccgga
      961 aaacgacatg tattttgcgt ttgcgggcga cgaggaagtc gccggcaaag cgcagccgga
     1021 gatcgttgaa acgctccgca gccgcggcgt cgttcccgcc atcgtggtgg acgaaggcgg
     1081 cgcggtggtg gaaggcatct tccccggcgt caaagcgccc tgcgcgctga tcggcattgc
     1141 ggaaaaaggg ctcatggatg cgcagttcgt aatcgaaggc gcgggcggac acgcttccgc
     1201 gccgccgccg cataccggtc tcggccgcat tgcgcgtgcc gtttgcagcg tggagaacaa
     1261 gccgttcccg cgcgtgctga ccaagccgat ttccgagatg ttcgacacgc tggggcggag
     1321 ctcaacgttc ctttaccgcg tgatcttcgc aaacctctgg tgctttttgc cgcttttaga
     1381 tgcattgtgc aaaaagaccg gcggcgagct caacgccatg atgcgcacca cctgcgcgtt
     1441 taccatggcc gaaggcagca agacttccaa cgttctaccg ccgcgcgtct ccgttgtggc
     1501 gaacctgcgg ctcatctccg gcacgacccg cgaacagacg ctggacatgc tgcgtgagcg
     1561 cgtggaggac aaggatatca aagtctccat gctgcacggc atggatccct gcaagtgctc
     1621 cgaaacggag ggctacggct gggaaaaact caagtccgcc attgccgaaa cctggccgga
     1681 cgcgctgatt tcgccgtacc tgatgattgc ggcttccgat tcgcgccatt act
//
LOCUS       DAOMGY010000410         1732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_80702_length_1732_cov_3.476446, whole genome shotgun sequence.
ACCESSION   DAOMGY010000410 DAOMGY010000000
VERSION     DAOMGY010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80702_length_1732_cov_3.476446"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..>1732)
                     /locus_tag="PLR57_06665"
     CDS             complement(214..>1732)
                     /locus_tag="PLR57_06665"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HQQ41175.1"
                     /translation="TPGITSGRIVTDPFTARVFFQNETKGKENDAEVSVGISPQPVFS
                     ETGLYQTDPISVSITCSDPNAAIYYTTDGDEPSTKSHLYSGPVTVSESTPLRAVSVVA
                     GKLVSDITTATYLFVEPHTVPVVCITGNAKRINEVMRVDDKDEKVERLAYLSYYEADG
                     ELGISFPAGIKPKGAGTIVYAQKSLSVNLRAGYGQSSVTYPFWDDYEFKTFSSLVLRN
                     GGQDWSAARIRDSFISRLVEGMYVDNSATRPVVVYINGEYNGLYDLNDDLNSEFLVTH
                     YGIDGDTVDFIRRNEAVIKGSNKDIRRVRTIGSNSDLSDDEKFAEYSQWVDVTYFTDY
                     YIATTYICNSDMFNQKYWRTQDYTLKWRPVFFDLDFGFKSASRDMLGKFFNPKGEASP
                     DQSKTYFEIYIGLKKNAAWRDYCVERYVEVVETYFNSERATALLDEMTAVLRPEIQRQ
                     IDKWHRPYSMQEWEDSIAELREIVAQRPEYALQNLQDYFRVSQEKMDELIAKYSK"
BASE COUNT          374 a          450 c          457 g          451 t
ORIGIN      
        1 tataaaatca acttatttta gcatgaaacg ggttgcctgt aaatgaacgg cactgcgatg
       61 acggattgtt tccttattgt tttatagttc attcacaact tgccgctttg tgccattttt
      121 aagcaacaac agcaaaaacc gcgcttcagc gcggtttttg ggtttgatgt tcgcttttcc
      181 agtgtaaagc tttgtcctgc tccatagcaa acgctatttc gagtacttgg caatcagttc
      241 gtccatcttc tcttgagaga cacggaagta atcctgcagg ttttgcagcg cgtattccgg
      301 ccgttgggct acgatctccc gcagttccgc aatggaatct tcccattcct gcattgaata
      361 cggacggtgc cacttgtcga tctgacgctg tatttcgggg cgaagcacag ccgtcatttc
      421 gtccagaagc gcggttgcgc gttcgctgtt aaagtaggtt tcaacaacct ccacataccg
      481 ctcgacgcag tagtcccgcc atgcggcgtt tttctttagc ccgatataga tttcgaagta
      541 ggtcttcgac tgatcgggtg aagcctcgcc tttgggatta aaaaactttc cgagcatatc
      601 ccgcgatgcg ctcttgaagc cgaagtccag gtcaaagaat accggtctcc actttagtgt
      661 atagtcctgc gtacgccagt atttctggtt gaacatgtcg ctgttgcaga tgtaagtcgt
      721 tgcaatatag tagtccgtaa agtacgtaac gtctacccac tggctgtact cggcaaattt
      781 ctcatcgtcg ctcaagtcgc tgttcgagcc aatggtgcgc acacgccgga tatctttatt
      841 cgagcctttg atgaccgctt cgtttctgcg gataaagtcc accgtgtccc cgtcgatgcc
      901 gtaatgcgtc acgagaaatt ccgagttcag gtcgtcgttg agatcgtaaa ggccgttgta
      961 ttcgccgttg atgtagacga ccacggggcg ggttgcggag ttatccacgt acatgccttc
     1021 cacaaggcgt gagataaacg aatcgcgaat gcgcgcggcg gaccagtcct gaccgccgtt
     1081 gcggagcacc agcgaagaga aggttttaaa ctcgtaatca tcccaaaacg gatacgtcac
     1141 gctgctctgg ccgtaacccg cgcggaggtt gacgctcagc gacttctggg catagacaat
     1201 cgtacccgcg cctttgggct tgatgcctgc cggaaacgaa atgccaagct cgccgtccgc
     1261 ctcgtaatac gaaaggtagg caaggcgttc cactttttcg tccttgtcgt ccacgcgcat
     1321 gacttcgttg attcgttttg cgttgccggt aatgcagacc accggaaccg tatgcggctc
     1381 aacgaaaagg tatgtcgcgg ttgtgatatc gctgacgagt tttccggcaa caaccgaaac
     1441 agcgcgcaga ggcgtactct cggaaaccgt gaccgggccg gagtataaat gggacttcgt
     1501 gctcggctcg tcgccgtcgg tcgtatagta gatggcggca ttgggatccg aacaggtaat
     1561 cgacacgctg atcgggtcgg tctggtacaa acctgtttcg gaaaacaccg gctggggcga
     1621 tatgccaacg ctgacttccg cgtcgttttc tttgcctttt gtttcgtttt gaaagaaaac
     1681 gcgcgcggtg aacgggtcgg taacgatcct gccgctggtg atgcccggcg ta
//
LOCUS       DAOMGY010000411         1730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_80865_length_1730_cov_3.112239, whole genome shotgun sequence.
ACCESSION   DAOMGY010000411 DAOMGY010000000
VERSION     DAOMGY010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80865_length_1730_cov_3.112239"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="PLR57_06670"
     CDS             <1..1466
                     /locus_tag="PLR57_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQQ41176.1"
                     /translation="SNTMFVAGITTLISVVLSVGMAYALTRYDIRIKRILNICIILSL
                     MSPPFIGAYSWILLFGRSGFVTTKALEWFGWVLPTIYGKWGIVSVFVCKMFPYVYLFT
                     SGALGSIDSSLEEAAENLGSTKGYRLFHVTLPLVSPSILAGAVMVFMSAVSDFGTPML
                     IGENYKVLSTLVYEQYMSEMGTSSNFASAVSMIVIALCLILLLLQRWYLRRRNYNMTS
                     LRPPQPIRLSVGKKILLLLPVLAVVLFSLLPQIVVIVTSFKNCNGPYFVAGWGLESYR
                     TIFTRMIRGIKNTYFFSLIALVMMVMIGSITAYLSVRKRSALTTAVDLSLMVPYVIPG
                     AVMGLALIIAFNRSPFFWAGTAFIMILCYTIRKIPYTVRSASGFLQQMELCVEEASIN
                     LGVSPARTFFKITLPLMLPGVVSGAILSWIETINELSSSVLLYTGKTITMSVAIYNEV
                     ARNSFGTAAAMATILTISAVIMLIVFNHVSKGKVSIV"
     gene            1532..>1730
                     /locus_tag="PLR57_06675"
     CDS             1532..>1730
                     /locus_tag="PLR57_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41177.1"
                     /translation="MNRLLHRGEDTKSAPSAAGQQNTRGAKRARSLHQSGVFRRLLVN
                     FLLIGLVPITVLAFLAIVYSTR"
BASE COUNT          318 a          491 c          475 g          446 t
ORIGIN      
        1 tgtccaacac gatgtttgtt gcgggtatta ccacgcttat cagcgtcgtg ctcagcgtgg
       61 gcatggcgta cgcgctgacg cgctacgata tccgcatcaa gcgcatactg aacatctgca
      121 tcattctttc gctcatgagc ccgccgttta tcggcgctta cagctggatt ctgctcttcg
      181 ggcgctcagg gttcgtcacc accaaggcgc tggagtggtt cggctgggtg ttgccgacta
      241 tctacggcaa atggggcatt gtgagcgtat tcgtctgcaa aatgtttccg tatgtctacc
      301 tgtttacctc cggcgcgctg gggagcatag actcctcgct ggaggaagcg gcggagaatc
      361 ttgggtcaac caagggatac cgtttgtttc atgtaacgct gccgctcgtc agcccctcca
      421 tcctggccgg cgcggtgatg gtgttcatgt cggcggtatc cgatttcgga actcccatgc
      481 tgatcggcga gaactacaag gtgctttcca cgctcgttta cgagcagtac atgagcgaga
      541 tgggcacgag ttccaacttc gcttccgccg tgagcatgat cgtcattgcg ctgtgcctga
      601 ttctgcttct gctgcagcgc tggtatctgc gccgccgcaa ttacaacatg acctcactgc
      661 gcccgccgca gccgattcgc ctttcggtcg gcaaaaagat tctgctgctc ttgccggtgc
      721 tggcggtcgt actcttttcg ctgctgccgc agattgttgt catcgtgacc tcgtttaaaa
      781 actgcaacgg cccgtacttt gtcgcgggct ggggactgga aagctaccgc acgatcttta
      841 cgcgcatgat acgcggcatc aaaaacacct atttcttctc gctgattgcg ctcgtaatga
      901 tggtgatgat cggctcgatt acggcgtatc tttctgttcg caagcgcagc gcgctgacca
      961 cggcggtcga tctttcgctc atggtgccgt acgtcattcc gggcgctgtg atggggcttg
     1021 cgctgatcat tgcgttcaac cgctcgccgt tcttctgggc gggaaccgcg tttattatga
     1081 tcctctgcta cacgattcga aagataccgt acaccgtgcg ctcggcttcc ggctttctgc
     1141 agcagatgga gctctgcgtg gaggaggctt ccatcaacct tggcgtttcg cccgcaagga
     1201 cgttttttaa gatcacgctc ccgctcatgc tgcccggcgt ggtttccggc gcaattttaa
     1261 gctggattga gacgattaac gaactcagtt cgagcgtttt attgtatact ggtaagacaa
     1321 tcaccatgtc cgtcgccatc tacaacgaag tggcgcgcaa cagcttcggc accgccgccg
     1381 ctatggcgac gatactcacc atttccgccg tcattatgct gatcgttttc aaccatgtaa
     1441 gcaaaggcaa agtctcgatc gtataaaaaa ttctattcat agtgtcccgg tttgagtcgg
     1501 ccgggggcaa aggcagggct gcagcgctat tttgaatcgc ttattgcacc gcggagagga
     1561 cacaaaaagc gctccctccg cagcgggtca gcagaatacg cggggcgcga aacgcgcgcg
     1621 ctcccttcac cagtcaggcg tcttccggcg gctgctggtg aattttctgc tcattggcct
     1681 cgtgccgatt acggtgcttg cctttcttgc aattgtgtat tccacgcgcg
//
LOCUS       DAOMGY010000412         1721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_81523_length_1721_cov_1.947779, whole genome shotgun sequence.
ACCESSION   DAOMGY010000412 DAOMGY010000000
VERSION     DAOMGY010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81523_length_1721_cov_1.947779"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..1624)
                     /locus_tag="PLR57_06680"
     CDS             complement(290..1624)
                     /locus_tag="PLR57_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41178.1"
                     /translation="MKRIAALLCALLFLFSMAACSKAPEPAAETPMPTATATATPAPT
                     EAPTPEPTPSPTPEPAFIQTALSEQLFARERVILTATGIRFSANYAAYIDLSIENASE
                     QAVSLALNGLYLNGWRVQGALENAESIQPGETRVSSITVLWTDDPTAVYMDISRIVSF
                     GCGLTLSKADDGEAIAAQKLITVSVPDAEPLPSPAEGATAVYEDNNLIVYLQGIDQSL
                     ALTRVILYKKPDAKWASATIDPVYAGYTNQVNGTYPLDAGMYQFLLFDGTDVMTSRNI
                     TSLSELDLYISLNMADGRINRPVIATITDPNVAETAVAAPDPGPIVYQSELEYCILKY
                     MGLITFEGHEAILLDFENVSQNYIKKLDMTAFGAIQTITVDGVNYPLGIYCTYAFPTT
                     HGYIMLWPEGAPEGTLSNAASASARLKIARINAGRLDPIQDTGTFTVDLKSK"
BASE COUNT          352 a          451 c          529 g          389 t
ORIGIN      
        1 tgtgccattg tcctgtgggg tgggggaaga ataacgttcg taccaatcgt tgttctgatc
       61 catattgtga cgctcctttt ttcgtctgac atcagtatag aaaatcaatt tgaactttca
      121 atacgcctgt tttgagcatt ctgtgttaaa tgtcgtcaat attatggcag tgaatgagag
      181 gggacaaaaa atggcggagc attcagctcc gccactttta atgatttgat tcttcgaggg
      241 ctccgccctc aagcacctgc ccaagggatg atcccttggg aatcccgctt tacttcgact
      301 tcagatcgac ggtaaacgtg cccgtgtcct gaatcgggtc gagacggccc gcgttgatgc
      361 gcgcaatctt gagccgcgcg gatgcgctgg cggcattcga aagcgtacct tccggcgcgc
      421 cctccggcca gagcatgata tagccgtgcg tggtcgggaa agcataggtg cagtagatgc
      481 ccagcgggta gttcacgcca tctaccgtaa tggtttgaat cgcgccgaac gcggtcatat
      541 ccagcttttt aatgtagttc tggctcacgt tttcaaagtc gagcagaatc gcttcgtggc
      601 cttcgaacgt gatgagcccc atatacttca gaatgcaata ctccagctcg ctctggtaga
      661 caatagggcc gggatccggc gcggcgactg cggtttcggc aacattggga tcggtaatgg
      721 tcgcaatcac gggccggttg attcgcccgt ccgccatgtt gagcgagatg tagagatcca
      781 attcggacag gctcgtaata ttgcgactcg tcataacgtc cgttccgtcg aacaagagga
      841 actgatacat gccggcgtcg agcggataag tgccgttcac ctgattggtg tatccggcgt
      901 aaacggggtc gatggtcgcg ctggcccact ttgcgtccgg ctttttgtaa aggatgacgc
      961 gcgtgagcgc gagcgactgg tcgatgccct gcaggtagac gatcaggttg ttgtcctcgt
     1021 agacggctgt agcgccttcc gcgggagagg ggagcggttc ggcgtccggc acggagacgg
     1081 taatcagctt ctgcgcggca atcgcttcgc cgtcgtccgc cttgctgagc gtaagcccgc
     1141 agccaaagga gacgatccgc gaaatgtcca tatacaccgc ggtcgggtcg tccgtccaca
     1201 gcacggtaat cgaagaaacg cgcgtttcgc cgggctgtat ggattccgcg ttctccagag
     1261 cgccctgcac gcgccagccg ttcagataca gcccgttcag cgcaagactg accgcttgtt
     1321 cggaagcgtt ttcaatggaa aggtcgatat acgccgcgta gttggcgcta aagcgaatac
     1381 ccgttgccgt caagatgacg cgttcgcgcg caaagagctg ctcggagagc gcggtttgaa
     1441 taaacgcggg ttccggcgtc ggcgagggcg tgggttccgg cgtaggcgct tcggtcggcg
     1501 cgggggtcgc cgtggcggtt gccgttggca tcggggtctc ggcggcgggt tcgggcgctt
     1561 tggaacaggc ggccatactg aacaggaaga ggagagcgca gagcagcgcg gcgatgcgtt
     1621 tcataagaaa tagctctttt ctgtttcatt catgcgaacc attccgccgc cggcagccga
     1681 aaaaacggct ggcacggcga aaattgttcg cgctagaata a
//
LOCUS       DAOMGY010000413         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_81590_length_1720_cov_2.284084, whole genome shotgun sequence.
ACCESSION   DAOMGY010000413 DAOMGY010000000
VERSION     DAOMGY010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81590_length_1720_cov_2.284084"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /gene="cpaB"
                     /locus_tag="PLR57_06685"
     CDS             <1..744
                     /gene="cpaB"
                     /locus_tag="PLR57_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HQQ41179.1"
                     /translation="AAGALLYIYLGKLEQKNEVTVVYEDVLVAAADIPAYSAITEEMV
                     TVKQVPQGTSHPLAARTPAEAVGFVTENLIVEGEEILPVKLKQPGQTESGLSYVVPEG
                     MRAITVPVDEISGVAGFIQRGDYVDVLAFISVKVLSYNGMDPAVYKAATGLDAPAEEL
                     TESEENATVVVAQNICVAAVGTTFEPAADGLEEAYTSVTLYVTPEDAMRINQCGKSGP
                     LTLVLRASGDHEKNTEDAILSNQLLEKAK"
     gene            744..>1720
                     /locus_tag="PLR57_06690"
     CDS             744..>1720
                     /locus_tag="PLR57_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQQ41180.1"
                     /translation="MESRVNSIRIMLAAFSADDRKAAAGLLQDEALRIVCSLRPDKDG
                     LKKAGTQPADVLLLVTRGDPEAEFDFAERMYTNRSDLTLLLLMPRPEPADMERAMESG
                     IARVIDMNSGVEQIKTHIFTAVNRDRHRRSSLAKVATYDSKIAAFFSAKGGVGKTTMA
                     VSLACALAAQNKKVALIDLNLQFGDVGVFLDITKGDTIADMVEEHTFELATMKSYLIR
                     HYTGVMVMLSSSSPEYAELIRPEHIEAILSTLRPEFDYIVLDMGVTLSDCAISAFELA
                     DTVYFVVNEDIACLHDAKRSLKILEALNLSDKIKVVVNKDGISNIKVKDV"
BASE COUNT          394 a          492 c          505 g          329 t
ORIGIN      
        1 gccgcaggcg cgctgctcta catctatttg ggcaagctgg agcagaaaaa cgaagtaacg
       61 gtcgtctatg aggacgtgct tgtcgcggcg gccgatattc cggcctactc cgccatcacg
      121 gaagaaatgg tcacggtcaa gcaggtgccg cagggaactt ctcacccgct cgccgcacgc
      181 acccccgcgg aagccgtcgg ttttgtcacc gagaacctga tcgttgaagg cgaggaaatc
      241 ctgcccgtca agctcaaaca gcccggccag accgaaagcg ggctttcgta tgtcgtgccg
      301 gaaggcatgc gcgcaattac cgttcccgtg gacgaaatct ccggcgttgc cgggtttatc
      361 cagcgcgggg actacgtgga cgtgctggcg tttatctccg taaaagtgct cagctataac
      421 ggcatggatc ctgcggtcta caaagccgca accgggctgg acgcaccggc ggaggagctg
      481 accgaatccg aggaaaacgc aacggttgtc gtggctcaga acatctgcgt cgcggcggtc
      541 ggaacgacgt ttgaacccgc cgcagacggc cttgaggaag cgtataccag cgttacgctg
      601 tatgttacgc ccgaggacgc catgcgtatc aaccagtgcg gaaaatccgg cccgctgacg
      661 ctggttctgc gcgcttctgg cgaccacgaa aagaatacgg aagacgcgat tctcagtaat
      721 cagcttttgg agaaggcaaa gtaatggaaa gcagagtgaa cagcattcga atcatgctgg
      781 cggcattctc cgccgacgac agaaaagctg ccgcggggct gctgcaggac gaagcgctgc
      841 gcattgtctg ctcgctccgt ccggataagg acggcctgaa aaaagccggc acgcagcctg
      901 cggacgtcct cttgctcgtt acgcgcgggg atccggaagc ggagtttgat tttgccgagc
      961 gcatgtacac aaaccgcagc gaccttaccc tgctgctgtt aatgccgcgc cccgaacccg
     1021 ccgatatgga gcgggctatg gagagcggca ttgcgcgcgt tatcgatatg aacagcggcg
     1081 tggagcagat taaaacgcat atcttcaccg ccgtcaaccg cgaccgccac cgaaggagca
     1141 gccttgcaaa ggttgcgacc tacgactcca agatcgcggc tttcttctcc gccaaaggcg
     1201 gcgtaggaaa gaccaccatg gcggtcagcc ttgcctgtgc gctcgccgcg cagaacaaaa
     1261 aagtcgcttt gatcgacctg aatcttcagt tcggcgacgt aggcgtattt ctggatatta
     1321 caaagggcga cacgattgcg gatatggtgg aagagcatac cttcgagctc gccaccatga
     1381 agagctacct catccggcac tacacaggcg tcatggtgat gctctcgagc agttcgccgg
     1441 agtacgccga attgatccgc ccggagcaca ttgaagcgat tctttccacg cttcggccgg
     1501 agttcgacta tatcgtgctg gatatggggg taacgctctc cgactgcgcc atctccgcgt
     1561 ttgaactggc ggacaccgtg tatttcgtcg tcaacgaaga tatcgcctgt ctccacgacg
     1621 cgaagcgcag cctaaagata ctcgaagcgc tcaacttgag cgacaagatt aaggtcgtgg
     1681 tcaacaagga tggcatttcg aacatcaagg tcaaggacgt
//
LOCUS       DAOMGY010000414         1713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_82094_length_1713_cov_2.860676, whole genome shotgun sequence.
ACCESSION   DAOMGY010000414 DAOMGY010000000
VERSION     DAOMGY010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82094_length_1713_cov_2.860676"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /locus_tag="PLR57_06695"
     CDS             complement(<1..1660)
                     /locus_tag="PLR57_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQQ41181.1"
                     /translation="MDVPQMAKAAAPAPKIKRKLVLLMVFAGILMLVLALRLTQVMIV
                     QGPDLQTKAKSQWTRKENLAAQRGRIIDRNGLVLAQSGTAYRVLANPEQIAADDRVRI
                     ATEVSDILGLDYDYVLERITPSTDPTAKKRLQVQLKRQVESSVVDQLQALQLGSGITF
                     TTDMKRYYPFGELFTQLIGFTGIDTEGQTGIEAEYDSYLAGEPGVLVTEVDRKNQPLS
                     YGKEDYVPPTDGYDLTITGDSVTQSYLERYVQQCYVATKAKSVSGIVMDPNTGEILAI
                     TSYPSFDLNDPPRDTVTELMSMSKNQIVTETYEAGTMFDLITVAAALDSGNTSLDKTY
                     KCSGSRIYRLQKITCWDTEGHGTQTLAEALGNSCGVAMMDIAGDMGVDTLYDYIYGFG
                     FGESTECGIPSEDTGEVIHRKYIRASDLAKLSFGETITMTPIEMASALCAAINGGVLM
                     KPYVIDSITDTDGNVILQNEPTILRRVISSSTSATLRSLMHSNVQKGNASNAQVINYT
                     VGGVTGVSRKFEEDGVTASRMRVVASFAGFLPIDNPQLLCMIVVD"
BASE COUNT          335 a          492 c          489 g          397 t
ORIGIN      
        1 cgtcaacaac gatcatgcag aggagctgcg ggttgtcgat gggtaggaat cccgcaaagg
       61 acgcgacaac gcgcatgcgc gacgcggtaa cgccgtcttc ttcaaacttg cgagaaacgc
      121 cggtgactcc gcctacggtg tagttgatga cctgcgcgtt ggaagcgttg cccttctgca
      181 cgttggagtg catcagcgaa cgcagcgttg cggacgtgga agagctgatg acgcgccgta
      241 aaatggtcgg ttcgttctgc aggatgacgt ttccgtcggt atccgtaatg ctgtcgatta
      301 catacggctt catcagtacg cccccgttga tggccgcgca gagcgcgctc gccatttcaa
      361 tgggcgtcat ggtgatcgtt tcgccaaagg acagctttgc cagatcgctt gcgcgaatgt
      421 acttgcggtg aatgacttcg ccggtgtcct cgctgggaat gccgcactcg gtggattcgc
      481 cgaagccgaa gccgtagata tagtcgtaga gtgtgtcaac gcccatatcg cccgcgatat
      541 ccatcatcgc cacaccgcag gaattgccca gcgcttccgc gagcgtctgc gtgccgtggc
      601 cttccgtgtc ccagcaggta atcttctgca ggcggtagat gcgcgaaccg ctgcatttat
      661 aggttttatc cagggatgtg tttccgctgt ccagcgcggc ggcaaccgta atcaaatcga
      721 acatcgtgcc cgcttcatac gtttccgtta cgatctggtt tttcgacatg gacatcagtt
      781 cggtaaccgt gtcgcgcggc ggatcgttca agtcgaacga cggataggaa gtaattgcga
      841 ggatttcgcc ggtattcggg tccataacaa tcccgctgac gctcttggcc ttggttgcca
      901 catagcattg ctggacgtac ctttcgagat agctctgcgt taccgagtcg ccggtaatcg
      961 tcagatcgta gccgtccgtc gggggaacat aatcttcctt gccgtaggaa agcggctggt
     1021 tctttcggtc tacctcggtc accagaacac ccggttcacc cgcgaggtag ctgtcgtatt
     1081 ccgcttcaat ccccgtctgg ccttcggtgt cgatgcccgt aaaaccgatc agctgcgtaa
     1141 aaagctcccc gaacgggtag tagcgcttca tgtcggttgt aaacgtaata ccgctgccca
     1201 gctgcagagc ctgaagctgg tccacgaccg agctttccac ctgccgcttg agctgcacct
     1261 gcagacgttt cttcgcggtc ggatcggtgc tgggcgtgat gcgctcgaga acataatcgt
     1321 aatcgaggcc gagaatatcc gaaacctcgg tcgcaatgcg gacgcggtcg tccgccgcga
     1381 tctgctcagg gtttgcaagc acgcggtaag ccgtgccgct ctgcgcgaga acaaggccgt
     1441 tgcggtctat aattctgccg cgctgggcag ccagattttc ttttcttgtc cactgggatt
     1501 tggccttcgt ctgaagatcc ggcccctgga cgatcatcac ctgcgtcaac cggagcgcga
     1561 gaaccagcat gagtatgccg gcaaatacca taagaagcac aagcttccgt ttaatcttcg
     1621 gtgcgggtgc tgctgctttc gccatttgag gaacgtccat tcgttaggat caaccgccag
     1681 aaggcggctg gctcccccca cccgtcgtat ctc
//
LOCUS       DAOMGY010000415         1713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_82112_length_1713_cov_2.121834, whole genome shotgun sequence.
ACCESSION   DAOMGY010000415 DAOMGY010000000
VERSION     DAOMGY010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82112_length_1713_cov_2.121834"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="PLR57_06700"
     CDS             <1..1175
                     /locus_tag="PLR57_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41182.1"
                     /translation="DEESNVMFGEGGAGTFSDGKLTSRSKDARGDDVLSTLVHFGAPE
                     EIAVSAKPHIGTDRLRSVVSNLRKEIERLGGEVHFGAKLNGISFTKGAVSRVLFTQNG
                     AEESADCAALVLAIGQGARDTYQMLFDSGVMMAKKPFAVGLRIEHAQSMIDRVQYCAF
                     AGHPRLGAAEYRLTAQSGDRGVYTFCMCPGGSVIASASRRGEVVVNGMSNLARNAENA
                     NAAIVVQVRESDLPEDAFAGVRFQKEMERAAFLAGGADGTAPASTVGAFVSRSAAKGF
                     GGIKPSYRPGVKAVDLWGVLPSFVSKGIADGVAAFGRQLKGFDEPDAVLTGVETRTSA
                     PLRILRGETMESVSCARLYPVGEGAGYAGGIVSAAIDGMKAAERIISLYAPPGREV"
     gene            complement(1217..>1713)
                     /locus_tag="PLR57_06705"
     CDS             complement(1217..>1713)
                     /locus_tag="PLR57_06705"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQQ41183.1"
                     /translation="KFREQCPVQRIPTLEEYFALVKEHDFLNIIELKTAIFEYDGIEQ
                     KVIDLVRAFGLSDRVVLSSFNHYSLLRCKAIAPELPCGILYECRIAEPQDYCKKLGMQ
                     YLHPDYRFLDDAELAKYESAGVKTSPWTVDREEDMRYLLAQKNIFALMSNKPDLALSI
                     RDQK"
BASE COUNT          340 a          473 c          564 g          336 t
ORIGIN      
        1 ttgacgagga gtccaacgtg atgttcggcg aaggcggcgc tggcacgttt tccgacggaa
       61 aactcacctc gcgcagcaag gacgcgcgcg gggacgacgt gctcagcacg ctggttcact
      121 tcggcgcgcc ggaagagatt gcggtttccg ccaagccgca tatcggcaca gaccgtctcc
      181 ggagcgtcgt ttccaacttg cgcaaggaaa ttgagcgtct cggcggggaa gtgcactttg
      241 gcgcgaagct gaacgggatt tcgtttacaa aaggcgcggt aagccgcgtt ttgtttaccc
      301 aaaacggcgc tgaggagtcg gcggactgcg cagctctcgt gctcgccatc gggcagggcg
      361 cgcgggatac gtatcaaatg ctgttcgact cgggcgtgat gatggcgaaa aagccgttcg
      421 ccgtgggcct gagaattgag cacgcgcaga gtatgatcga ccgtgtgcag tactgtgcgt
      481 tcgcggggca tccgcgcctg ggtgccgcgg aataccggct cacggcgcag agcggcgaca
      541 ggggcgttta taccttctgc atgtgcccgg gcggcagcgt aatagccagc gcgtcgagac
      601 ggggcgaagt cgtcgtaaac ggcatgagca acctcgcccg aaacgccgag aacgcaaacg
      661 ccgccatcgt cgtgcaggtg cgcgagagcg acctgccgga ggacgcgttt gcgggcgtac
      721 gctttcaaaa ggagatggaa cgcgccgcgt ttctcgcggg cggcgcggat ggcacagctc
      781 ccgcaagcac ggtgggcgcg tttgttagtc gatcggcggc aaaaggattc ggcgggataa
      841 aaccctccta ccgtcccggc gtcaaggctg tcgatctttg gggcgttctg ccttcgttcg
      901 tttcgaaagg gattgcggac ggcgtcgccg cgttcggcag gcagctcaag ggctttgacg
      961 agccggatgc agtgctcacc ggcgtggaaa cgcgcacgag cgcaccactg cggattctgc
     1021 gcggagagac aatggagagc gtctcctgtg cgaggctgta ccccgtaggc gagggggcag
     1081 gatacgcggg cggcatcgtt tccgccgcga tagacggcat gaaagcggcg gagcggatca
     1141 tctcgctcta cgcgccgcct ggcagggaag tataataaaa aaccgggcac tgcccggttt
     1201 tttgatacgg ttcgttctac ttctgatccc gaatggatag cgcgaggtcg ggcttgttgc
     1261 tcatgagggc gaagatgttc ttttgtgcca gcagataccg catgtcttct tcacggtcaa
     1321 ctgtccacgg gcttgtcttt acgcccgcgg actcatactt cgccagttcg gcgtcgtcca
     1381 gaaaacggta gtcggggtgc aggtactgca tgcctagctt tttgcagtag tcctgcggct
     1441 cggcgatgcg gcattcgtag aggatgccgc agggcagctc cggcgcgatt gctttacacc
     1501 gtaaaagtga ataatggttg aaggaggaca gcacgacccg gtcggaaagc ccgaacgcgc
     1561 gcacgagatc gatcaccttt tgctcaatgc cgtcgtattc gaagatcgcc gtcttcagct
     1621 cgatgatatt cagaaagtca tgctccttaa cgagcgcaaa atactcctcc agcgtcggaa
     1681 tgcgctgaac agggcactgc tcgcggaact tat
//
LOCUS       DAOMGY010000416         1712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_82162_length_1712_cov_2.869644, whole genome shotgun sequence.
ACCESSION   DAOMGY010000416 DAOMGY010000000
VERSION     DAOMGY010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82162_length_1712_cov_2.869644"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PLR57_06710"
     CDS             <1..1073
                     /locus_tag="PLR57_06710"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HQQ41184.1"
                     /translation="NGEQFEAEKPMKTHEDAIKLVLDVLTDKKIGVIEDMSCITAVGH
                     RVLHGGEKYSAPVLIDDQVLEAIEECIPLGPLHNPANLMGIRGCMAAMPGVPMVAVFD
                     TAWGQTMPKKAYMYALPYEAYTEHKVRRYGFHGTSHRYVSGRAIKKLEALGMKKEDTR
                     VITCHLGNGSSLSAVKGGKCVDTSMGLTPLEGVPMGTRCGSIDPAIMPYLMGRTGMSA
                     QEMDTYMNKKSGMLGLSGVSSDFRDLSAATKAGNERAALARELFCYKVKQYIGAYAAS
                     LGGVDAIVLTAGVGENDPFIREMILEGLEYLGMDMDYDYNKACPRGKEVEISKPTSKV
                     HVYVIPTDEEMTIAHDTAALAL"
     gene            1124..1291
                     /gene="scfA"
                     /locus_tag="PLR57_06715"
     CDS             1124..1291
                     /gene="scfA"
                     /locus_tag="PLR57_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HQQ41185.1"
                     /translation="MKHISTIQQPCIKSGKHTACGECQASCQSACKTSCTVANQKCAN
                     PRVAKENKQDR"
     gene            1424..>1712
                     /locus_tag="PLR57_06720"
     CDS             1424..>1712
                     /locus_tag="PLR57_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41186.1"
                     /translation="MIHSFQFQDQFLLVDVESGAIHKLDETAFRVASALEHGEDPYQT
                     GIAREAVSEVLADLEELRDSGSFEAKEVQAPEIHGGSAIKSMCLHVAHDCNL"
BASE COUNT          403 a          480 c          497 g          332 t
ORIGIN      
        1 agaacggcga gcagttcgaa gccgaaaagc ccatgaagac gcacgaggat gcgatcaagc
       61 tcgttctcga cgtgctgacg gacaaaaaaa tcggcgttat cgaagacatg agctgcatca
      121 ccgcggtcgg ccatcgcgtg ctgcacggcg gcgaaaagta ttccgcgccc gtgctgatcg
      181 acgatcaggt gctcgaggcc atcgaagagt gcatcccgct gggcccgctg cataaccccg
      241 ccaacctcat gggcatccgc ggctgcatgg ccgccatgcc gggcgttccc atggtcgccg
      301 tgttcgacac cgcctggggt cagaccatgc cgaaaaaagc gtatatgtac gcgctgcctt
      361 acgaagcgta taccgagcac aaggtgcgcc gttacggctt ccacggcact tcccaccgct
      421 atgtttccgg cagagcgatc aaaaagctgg aagcgctcgg catgaagaag gaagacaccc
      481 gcgtaatcac ctgccatctg ggcaacggtt cgagcctttc cgccgtcaag ggcggaaagt
      541 gcgtggatac ctccatgggc ctgacgccgc tcgaaggcgt acctatgggc acccgctgcg
      601 gcagcatcga ccccgccatt atgccgtacc tgatgggcag aaccggcatg agcgcgcagg
      661 agatggatac ctacatgaac aaaaagagcg gcatgctggg gctttccggc gtgtccagcg
      721 attttcgcga cctttccgcc gctacaaaag cgggcaacga acgcgcggct ctggcgcgtg
      781 agctgttctg ctataaagtc aagcagtaca tcggcgcgta tgccgcgtcg cttggcggtg
      841 tggacgcaat cgtactgacc gctggcgttg gcgaaaacga tccgtttatc cgcgagatga
      901 ttctggaagg cctggaatac ctcggcatgg atatggatta tgactacaac aaggcctgcc
      961 cgcgcggcaa agaagttgaa atttccaagc ccacgagcaa ggtgcatgtt tacgtgatcc
     1021 cgacggacga agagatgacc attgcgcacg atactgccgc gcttgcgctg taacggggat
     1081 tcgtgtataa tacttgagtg aattctattc ggaggaaaca gcaatgaagc atatttcgac
     1141 gatccagcag ccctgcatca agtccggcaa gcataccgcc tgcggcgaat gtcaggcgag
     1201 ctgccagtcg gcctgcaaaa cgtcctgcac ggttgccaac cagaaatgcg ccaaccccag
     1261 ggttgccaaa gaaaacaaac aggacagata agttcttcaa ctttcgccga tccgcaaaga
     1321 aaggccattt attcggcctt tctttgtctg ttgacgcggt atttcatgtt gcgcggtttt
     1381 cgtttgccgg gagcaaagag cagttcgcgc ggaaaggtgc gctttgattc attcctttca
     1441 atttcaagat cagtttctgc ttgtcgatgt agaaagcgga gcaatccata aactggacga
     1501 aacggctttc cgcgttgcgt ccgcgctgga gcacggggaa gacccgtatc agacgggaat
     1561 tgcgcgcgaa gccgtgagcg aagtgcttgc cgatctggaa gaactccgcg attccggctc
     1621 ttttgaagca aaagaagtgc aggcgccgga gatacacggc ggcagcgcga tcaagtccat
     1681 gtgcctgcac gtggcgcacg actgcaacct ca
//
LOCUS       DAOMGY010000417         1707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_82582_length_1707_cov_2.655569, whole genome shotgun sequence.
ACCESSION   DAOMGY010000417 DAOMGY010000000
VERSION     DAOMGY010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82582_length_1707_cov_2.655569"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    complement(220..466)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(652..1707)
                     /locus_tag="PLR57_06725"
                     /pseudo
     CDS             complement(652..1707)
                     /locus_tag="PLR57_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496974.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
BASE COUNT          297 a          515 c          487 g          408 t
ORIGIN      
        1 gtagacccgc cgcaccgttg cagccaggcg ttctgtctgc agcgcgcgga tcgtttcgcg
       61 cggggcggtc tcgatctccc gctgataata gtgttccatc gctcgtgatc tccttattcg
      121 cacgggcgaa gtgcttccgc ccgcaaatta cccaaaactg cgctcgcgcg ccttcgggtt
      181 cgcggcggat atcaaaaaac ccgtccgccg ctgtctccag cagaggacgg gcatagtccc
      241 gtggtaccac ctctgttcgc cgcaaccgtt gccgatgcgg cctcgtatgg atacgctgtc
      301 atatccacgg cgctgtatcg ggcgctcccg tctttgccta cttttgcctt ttcagcaagc
      361 cgctcgggaa ggaattcccc gtcccctctt tgcccgcctt ttcaccaacc ggcggctctc
      421 tttacaaagc gtttgggcga gtactggttt ccgtcttcgc gttgtgttga attcctgtct
      481 ttctgccgct gctgcggcat gaagcagaac cctgcaattt cgtttactat tatctcttgc
      541 agtcgctgta aagcgacgac tgcctatgat ttcgtttact ctagcacgct ttttggcggg
      601 tgtcaatagt atatgatata ctttcccgcg taattccgcc tgttgttccg ttcagctcac
      661 gcgctcgacc cagccgcgcg tatcctccag tttgccggtc tggatgccgc tgagcgtgtc
      721 gtagagcttc tgggaaagcg gccgacttcg tggttgttga tggcgtagtc gtcgcccttg
      781 tagcagagcg tgccgatggg cgaaataacg gccgccgtgc cggttgcaaa cacttccttg
      841 agcgtgcctg ccttcgccgc gtcaaaaagc tcgctgagcg acagtctccg ctggctgact
      901 ttgatgcccc attcgccaag cagcgtgatg acgctgtcgc gcgtgatgcc cggcaggatc
      961 gtgccgccca ggggagcggt gatcacctcg tcgccgatct tgaaaaacgc gttggaagtg
     1021 ccgatctctt ccacatactt gtgttcctgc gcgtcgagcc agagaacgtc cttgcagtgg
     1081 taagcctgtg ccttctggct ggccttcata ccgcccgcgt agttgccgcc gactttggca
     1141 aagccggtgc cgccctgcgc cgcgcggatg aactcgtcct ccacgtagat gcgggtcgcc
     1201 gaaagcttgc ccatgttcgc cgcgtagtat gggccgctgg ggcaaaggat gatcatgaag
     1261 cggtagtgct ttgccggcag gacgctgaag gacacctcgt ccgaaatgat gaacgggcgc
     1321 acatagagcg acgtaaaggg cttgctcggc acccagtcga tatccacgtt gacaacggcc
     1381 ttaaccgccg caacgaagag ctcctcgtcc atgggcggca tgcacatgcg ctcgttggtg
     1441 cggttgaggc gctgggcgtt catatacggg cggaagagct gaacgtgccc gtccgcggtt
     1501 ttgtaagcct tcatgccctc aaacatcatc tgcgcgtagt gcagaacgca gcttgccggg
     1561 tcaatggaga tatcgccgta agggataatc cgcccgtcgt gccagccttg tccttcgtcg
     1621 tagtccatca caaacatatg gtcggagaaa tgctggccaa acaccagatg atcttgatcc
     1681 ggcttctgct tgggattcgg gttgcgt
//
LOCUS       DAOMGY010000418         1707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_82603_length_1707_cov_2.052663, whole genome shotgun sequence.
ACCESSION   DAOMGY010000418 DAOMGY010000000
VERSION     DAOMGY010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82603_length_1707_cov_2.052663"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLR57_06730"
     CDS             <1..229
                     /locus_tag="PLR57_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41187.1"
                     /translation="ELLQRGRAVIQNVLFHVVQSIESHAGSSFPVDARAVGDAHSLPP
                     DVFVVFGTPKNSISAIITAFWVNCTDLFDKF"
     gene            complement(298..1134)
                     /locus_tag="PLR57_06735"
     CDS             complement(298..1134)
                     /locus_tag="PLR57_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003177805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQQ41188.1"
                     /translation="MKICFNQATTMKHSTLETDLAYCEKYHYDLIEIRLDKLHDYLTR
                     HTLGELKAFFDSHHIKPFAFNALEFINFRDKKGFEDIMRGLQFCCEAGHIIGCKRVVI
                     VPTFDIGNKTITEIREETVRVIHIMADYAEPRGMTLAFEFVGYPNCSVNTFGQAYDIV
                     KAVDRKSVGVVLDCFHFHAMGSRFEHLEAADASKIFILHLDDSEDLPVGAARDNNRLM
                     PGEGAVDLPRILSTLKRLGYDSMVSIELFRPEYWDWPDEENIRVSYDKTCETIKPYFP
                     IG"
     gene            complement(1395..>1707)
                     /locus_tag="PLR57_06740"
     CDS             complement(1395..>1707)
                     /locus_tag="PLR57_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41189.1"
                     /translation="TYCWSSFCAALAGVMVTARSASGIASLGDGYELDGIAAATVGGV
                     SQTGGVGSIIGCLGGILVLGVINNGLMITGVSPYLQKIIKGIIIVVAVVFDMRKVARK
                     R"
BASE COUNT          384 a          443 c          502 g          378 t
ORIGIN      
        1 agagctcctc cagcgaggaa gagccgtgat ccagaacgta ttgttccatg tcgttcagtc
       61 gatcgagtcg cacgcgggta gttcctttcc tgttgacgca cgagcggtgg gggatgcgca
      121 ttcgctgccg ccggatgtgt ttgttgtttt cggaacgccg aaaaacagca ttagtgccat
      181 tataacagcg ttctgggtaa attgcactga tctttttgat aagttttgat gcagtttacc
      241 cgaaacagca gtcgggagtt cactctgccg gatgcaatcc gatcattaag cgcatgttca
      301 gccgatgggg aagtacggct tgatcgtttc gcaggtcttg tcgtagctca cgcggatatt
      361 ttcctcatcc ggccagtccc agtattcggg gcggaacagc tcaatggaga ccatgctgtc
      421 gtagcccaga cgcttgagcg ttgagaggat acggggcagg tcgaccgcgc cttcgccggg
      481 catgaggcgg ttgttgtccc gcgcggcgcc gacgggcaga tcttccgagt cgtccagatg
      541 cagaatgaaa atcttgctcg cgtccgccgc ttccaaatgc tcgaagcgag aacccatggc
      601 gtggaagtgg aagcagtcca gcacgacgcc gacgctcttg cgatccacgg ctttaacgat
      661 atcgtacgcc tggccaaagg tattgaccga gcagttcggg tatccgacaa attcgaaggc
      721 aagcgtcatg ccacgcggtt cggcgtagtc cgccatgatg tggatcacgc gcacggtttc
      781 ctcgcggatt tcggtaatcg tcttgttgcc gatatcgaac gtgggcacga tgacgacgcg
      841 cttgcagccg atgatatgcc ccgcttcgca gcagaactgc agtccgcgca taatgtcttc
      901 aaagcctttt ttgtcgcgga agttgataaa ttccagcgcg ttgaacgcaa acggcttgat
      961 atgatgcgaa tcgaagaacg ccttcagttc gccgagggta tggcgcgtca ggtagtcgtg
     1021 cagcttgtcc aaccggattt cgatgagatc gtaatggtac ttttcgcagt acgccaaatc
     1081 ggtttccagc gtggaatgtt tcatggtcgt cgcttgattg aagcagattt tcatacagaa
     1141 tattcctccg tttgctgggt gaaatgaaaa atctatttgc gggaacgcag aacgcgtttt
     1201 acagttggaa aagaatggaa tagaacgctg atcaatggaa cagaggatcg ggtagaaata
     1261 ttaccgtttg cagccgcttg tttaaagcgg ctgcaaacgg ctaaggagga gaagaaaacc
     1321 catcccgatg agctaaacag gagcgcgcat gtgctgcgca gcacccgccg ccgtgaggcg
     1381 gcataaggag cggtttatct cttgcgggca acctttctca tgtcgaacac cacggcgacc
     1441 acgatgatga tgcccttgat gatcttctgc aggtaaggcg aaacgcccgt tatcattagg
     1501 ccgttgttga tgacgccgag caccagaata ccgccgaggc agccaataat ggagccgacg
     1561 ccgccggtct gggaaacgcc gccgacggtg gccgccgcga tgccgtccag ctcgtagccg
     1621 tcgccgaggg aggcgatgcc gctggcgctg cgcgccgtga ccataacgcc cgcgagagcc
     1681 gcgcaaaacg aggaccagca gtaagtt
//
LOCUS       DAOMGY010000419         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_84079_length_1687_cov_2.995098, whole genome shotgun sequence.
ACCESSION   DAOMGY010000419 DAOMGY010000000
VERSION     DAOMGY010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84079_length_1687_cov_2.995098"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PLR57_06745"
     CDS             complement(<1..963)
                     /locus_tag="PLR57_06745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HQQ41190.1"
                     /translation="MRITCTQELHDRGGPGLFLLARAENALETLLPDYAGKVQVIYLD
                     PPFGTGDVFHVKLTAGKRSIKLPAYSDKMREDEYLRWMKTILTGAKKLLSDTGSIYLH
                     IDFRMSAKLRLLMDEVFGAQNFMNEIVWCYKSGGRSTRYYPRKHDTILFYRKSANVFF
                     NIQAVGRPRGPEKRNHMRRFIDEEGRIAFTIRSAGKVYTYFEDSPVFPSDVWDDIEHL
                     QQKDKERVGYATQKPEALLNRVILASSNPGDLVCDLFSGSGTTAAAASKLGRRFLAVD
                     ASPFALYLLRARQLQNTSAVSLLEGEHAMRLCYPADETEAAFSAK"
     gene            complement(1028..1681)
                     /locus_tag="PLR57_06750"
     CDS             complement(1028..1681)
                     /locus_tag="PLR57_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQQ41191.1"
                     /translation="MAGFYDSKDYSSLSEKIYRSLRDGIAEGKYRTGDCLVEMKIAEE
                     LGVSRTPVREALKQLELEDLVASHPNRGVVVKGFSNEDYQDVSTIRHLLEGQAAYWAA
                     QRITDELLGELKDVVELMEFYTQKNDVEHLVRLDTRFHEIVYEASGSRTIRHILASLH
                     HNIRKARQSSLTLPERAACSLSEHQKIFSALESHDAQAAKEFMESHVAIAAGSRPNE"
BASE COUNT          337 a          465 c          512 g          373 t
ORIGIN      
        1 ctttgcggag aacgcagctt ccgtttcgtc cgcaggataa caaagccgca tcgcgtgttc
       61 gccttccagc agggagaccg cgctggtatt ctgcagttgg cgcgcgcgca gcaggtacag
      121 cgcaaacggc gaagcgtcca ccgcgaggaa acgcctgccg agcttgcttg ctgccgcggc
      181 ggtcgttccg ctgccggaga acaggtcaca gaccagatcg cccgggttgg aggaagcgag
      241 aatcacgcgg ttgagcagcg cttccggctt ttgagtggcg taaccgacgc gctccttgtc
      301 cttttgctga agatgctcaa tatcgtccca aacgtcgctg gggaaaaccg gtgaatcttc
      361 aaaataggta tagacttttc cggcggagcg gatggtaaaa gcgattctgc cttcctcgtc
      421 gataaaacgc cgcatatggt tgcgcttttc cggcccgcgc gggcggccga ccgcctgaat
      481 attgaaaaat acattggcgc tcttgcggta aaacagaatc gtatcgtgct tgcgcgggta
      541 gtagcgcgtg cttctgccgc cggatttgta gcaccagacg atctcgttca taaagttctg
      601 cgcgccgaaa acttcgtcca tcaaaagccg gagcttggcg ctcatgcgga agtcgatatg
      661 caggtaaatc gaaccggtgt cgctgagcag ttttttggct ccggtcagaa tcgttttcat
      721 ccagcgaaga tactcgtcct cgcgcatctt gtcggaatag gcgggcagct tgatgctgcg
      781 ctttcccgcg gtcagcttga cgtgaaacac gtcgcctgtg ccgaacggcg ggtcgaggta
      841 gatcacctgc accttgccgg cgtaatcggg aagcagcgtt tccagcgcgt tttccgcgcg
      901 cgcaagaaga aacaacccgg gaccgccgcg gtcatgcagc tcttgggtgc aggtaatgcg
      961 catgattgtg cctccgatct gaacggttga tgaaacgact ggaaaagaaa aagcgggacg
     1021 cggcgaacta ttcgttcggc cgggatcccg ccgcgatggc gacatgcgat tccataaatt
     1081 ctttggcggc ctgcgcgtcg tggctttcaa gcgcgctgaa aatcttctgg tgctccgaga
     1141 gtgagcaggc ggcgcgctcc ggcagggtaa gcgaactctg gcgcgccttg cggatattat
     1201 ggtggagcga agcgaggata tgccttatcg tgcggctgcc gctcgcttcg tagacgatct
     1261 cgtgaaagcg ggtgtccagg cggacgaggt gctccacgtc gtttttctgc gtgtagaact
     1321 ccatgagctc cacaacatcc ttgagttcgc ccaacagttc atctgtaatg cgctgcgccg
     1381 cccagtaggc cgcttggcct tccagcaggt gacggatggt ggagacgtcc tgatagtcct
     1441 cgttggaaaa gccttttacc acgacgccgc ggttcgggtg ggaggccacg aggtcttcca
     1501 actcgagctg cttgagcgct tcgcgcacgg gcgtgcgact cacgccgagc tcctcggcga
     1561 tcttcatttc gacgaggcag tcgcccgttc ggtatttgcc ttcggcaatg ccgtcgcgaa
     1621 ggcttctgta aattttttcg gaaagagaag aatagtcttt tgaatcgtaa aatccggcca
     1681 tacaggt
//
LOCUS       DAOMGY010000420         1682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_84504_length_1682_cov_3.430240, whole genome shotgun sequence.
ACCESSION   DAOMGY010000420 DAOMGY010000000
VERSION     DAOMGY010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84504_length_1682_cov_3.430240"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLR57_06755"
     CDS             <1..227
                     /locus_tag="PLR57_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41192.1"
                     /translation="DRHAVEAKRTFYFRSARDLDDAVLTVTKGSTTLLKKKFAHLRPP
                     EMERLTLSVENDQLTGEEPILFHLEANANA"
     gene            220..603
                     /locus_tag="PLR57_06760"
     CDS             220..603
                     /locus_tag="PLR57_06760"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HQQ41193.1"
                     /translation="MRDTIQLSEKEIVCIVCPNGCRIRCTPNGSEYSFSGNKCKRGIA
                     YVEAELTRPMRSLTSSVKTVFSNAPVVSVRTDGEIDKSRIHDVLSALGKVTLDHPVKM
                     GDVIIPNVCNTGVNLICTSNRLASH"
     gene            649..>1682
                     /locus_tag="PLR57_06765"
     CDS             649..>1682
                     /locus_tag="PLR57_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQQ41194.1"
                     /translation="MSHAYKGFEPNWVRGEFPKDSYRSIFKWGDLLEIKPPRESLYRM
                     MKAKFGLTDDDFKAYTEDLGFDKVEFNLPIQLKERDLESFRAIMGSEFVRTDDYARLS
                     VAYGKTMYDILRLRNKIVENVPDAVLYPDTKEQIEQVVAYCAKHKIPVYVYGGGSSVT
                     RGVECVKGGISLDMRLRFNKVIEFNEIDQTITVEAGMSGPKLEETLNNAVKLFGAKRA
                     YTCGHFPQSFEYSSVGGWVVTRGAGQNSTYYGCISDIVLAQEYATPIGVLKTDRYPRK
                     ATGSDLGQILMGGEGTYGVLTHVTLKVFRHMPETIKRFSYMFKDWETAQAAAREIMQS
                     EAGYPSVFRLS"
BASE COUNT          414 a          479 c          440 g          349 t
ORIGIN      
        1 tagatcgtca tgcagtggaa gcaaagcgta cgttctactt ccgttctgct cgggatttgg
       61 acgacgccgt tttgaccgtt acgaaaggca gtacaacgct gttaaagaaa aagtttgccc
      121 atcttcgccc gccggagatg gagcgcctga cgctttccgt tgaaaacgac cagcttaccg
      181 gcgaggaacc tatactcttc catctggagg cgaacgcaaa tgcgtgatac aatccaatta
      241 tccgaaaagg aaatcgtctg tatcgtctgc ccgaacggtt gccgtatccg gtgcacgccg
      301 aacggcagcg aatactcctt cagcgggaat aaatgcaagc gaggcattgc gtacgtagaa
      361 gcggaactca cccgccccat gcgctcgctg acatccagcg taaagaccgt cttttccaac
      421 gcgcccgttg tcagcgtacg caccgacggc gaaatcgata aatcccgcat tcacgacgtt
      481 ctctcagcgc tcggcaaagt tacgctcgac cacccggtca aaatgggcga cgtgatcata
      541 ccaaacgtct gcaacaccgg cgttaatttg atctgtacca gcaaccggct ggcaagccac
      601 tgataaggaa atcgtcattg acaactctga aaggagcgaa actattttat gtctcacgct
      661 tacaaaggat ttgaaccgaa ttgggtacgc ggcgaattcc ccaaggacag ctaccgctca
      721 atcttcaaat ggggcgacct gctggagatt aaacctccgc gcgaatccct ctaccgtatg
      781 atgaaagcga agttcggctt aaccgacgac gatttcaagg cgtatacgga ggatttgggt
      841 ttcgacaagg ttgaattcaa ccttccgatt cagctcaaag agcgcgatct ggaatcgttt
      901 cgcgccatca tgggcagcga gtttgtacgc acggacgact acgcgcgcct ttccgttgcc
      961 tacggcaaaa cgatgtacga tattctccgc ctccgcaaca agattgtcga aaatgtgccg
     1021 gacgcggttc tctaccccga tacgaaagaa cagattgaac aggtcgtggc ttactgcgcc
     1081 aaacataaaa tccccgtcta tgtctacggc ggcggttctt ccgtgacacg cggtgtggag
     1141 tgcgtcaagg gcggcatttc gctcgatatg cggctcaggt tcaacaaggt gatcgagttc
     1201 aacgagatcg accagaccat cacggtcgaa gccggcatga gcgggccgaa gctggaagaa
     1261 acgctgaaca acgccgtcaa gctctttggc gcaaagcgcg cctacacctg cgggcatttt
     1321 ccgcaaagct ttgagtactc ttccgtcggc ggctgggtcg taacccgcgg cgccggacag
     1381 aactcgactt actacggctg catttcggac atcgtactcg cgcaggagta tgcaaccccc
     1441 atcggggtgc tcaaaaccga ccgctacccg cgcaaagcaa ccggcagcga cttggggcag
     1501 attttgatgg gcggcgaagg cacctacggc gtactgacgc atgtcacgct caaagtgttc
     1561 cgccacatgc ctgaaacgat taagcgcttt tcctatatgt tcaaggactg ggaaaccgca
     1621 caggccgccg cgcgcgaaat catgcagagc gaagcgggct acccttccgt tttccgcctt
     1681 tc
//
LOCUS       DAOMGY010000421         1681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_84606_length_1681_cov_2.412054, whole genome shotgun sequence.
ACCESSION   DAOMGY010000421 DAOMGY010000000
VERSION     DAOMGY010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84606_length_1681_cov_2.412054"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLR57_06770"
     CDS             complement(<1..480)
                     /locus_tag="PLR57_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41195.1"
                     /translation="MDERNTNFTMADAFYAEFAKSAGISVKQLRTWTARTSFDSKFLH
                     LAGGRVSCEEVAGLCAPVMKKLSDLPAEGILPFAYESLSAGLYPYSGAPRADKGQKSA
                     LKFYLAVLSRLLDLERESAFDPLLDFLPVTEEEIAASPIANQCALFSETIKKCNYREL
                     "
     gene            complement(723..1139)
                     /locus_tag="PLR57_06775"
     CDS             complement(723..1139)
                     /locus_tag="PLR57_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQQ41196.1"
                     /translation="MRKILIDCREQIPCDPCRHACPSGAIIIEGDITNLPETFPEKCT
                     GCGLCVAACPGQACFLVDDAFAEGRASVDFPYEYLPLPEKGMTVSARDNEGSTVCDAV
                     VEDVILRKSAQGTAVVRVSVPKEFAYQVRGMKPLKR"
     gene            complement(1124..1447)
                     /locus_tag="PLR57_06780"
     CDS             complement(1124..1447)
                     /locus_tag="PLR57_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQQ41197.1"
                     /translation="MERDGMNAEIVCRCEEVTADEIMEAIRNGADSLEAVKKATRAGM
                     GFCQGRTCKRLVARMLACYYNMPVDRFLPGSLRLPVTPIRLDLLAETEDVPFTAKEGA
                     PCEKS"
     gene            complement(1429..>1681)
                     /locus_tag="PLR57_06785"
     CDS             complement(1429..>1681)
                     /locus_tag="PLR57_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HQQ41198.1"
                     /translation="ADMETTSKNILVAGDLTGIEEASTALDEGRMAGCRAAYALGYLS
                     EQEYEENKCVLLERLNALRCGPFGEKRRTAKEALWNAME"
BASE COUNT          318 a          529 c          476 g          358 t
ORIGIN      
        1 gagttcgcgg tagttgcact ttttaatcgt ctcggaaaac agcgcgcact gattcgcaat
       61 cgggcttgcc gcaatctctt cctccgtcac cggcaaaaag tccagcaggg ggtcgaacgc
      121 actctcccgc tccagatcga gcaagcgcga aagcacggcg aggtagaatt tcagcgcgct
      181 cttctggcct ttatccgccc gcggcgcgcc ggaatacggg tacagccctg cggaaagcga
      241 ctcataggca aacggcagga ttccttccgc gggcaggtcg ctcagcttct tcattaccgg
      301 cgcgcagagt ccggcgactt cttcgcacga aacgcgaccg cccgccagat gcaggaactt
      361 cgagtcaaag ctcgtccgcg ccgtccaggt gcgcagctgc ttgacggaaa tgcccgcgct
      421 tttggcaaac tccgcgtaaa aagcgtccgc catggtgaaa tttgtattgc gttcgtccac
      481 tgttgtgcct ccgttagcgc ggcggttggt ttccgttatc cgccgctgtc tgaactgcga
      541 tgctgataaa aagataggat ttgctttatc tgctagttct attctaataa ggaagagtca
      601 aagtgtcgag aaaaactatt atataaaata aggacgcccc atccgggacg cccgcgaaaa
      661 tatgtggttc tcaggggcgt gcccctgagc ggggtttggg gcggagcccc atcgttcttc
      721 ccttatctct tcagcggctt catgccgcgc acctgatacg caaactcctt tggtacgctc
      781 acgcgcacga ccgccgtgcc ctgcgcactt ttgcggagga taacgtcctc cacgaccgcg
      841 tcgcagacgg tgctgccctc gttgtcccgc gcggaaacgg tcatgccttt ctccggcagc
      901 ggcagatact cgtagggaaa atccacgctc gctcgtcctt cggcaaacgc gtcgtccacc
      961 aaaaagcagg cctgaccggg acacgcggca acgcagaggc cgcagccggt gcacttttcg
     1021 gggaacgttt ccggcaggtt cgtaatatcg ccctcgatga tgatcgcgcc ggaaggacac
     1081 gcgtggcggc atgggtcgca gggaatctgc tcgcggcagt cgatcaggat ttttcgcatg
     1141 gcgcgccctc ctttgccgta aacggaacat cctcggtttc cgccaagagg tcgagccgaa
     1201 tcggcgtgac cggcaggcgc aggctccccg gcagaaaacg gtcaaccggc atgttgtagt
     1261 aacaggcgag catccgcgcg acaagccgct tgcaggtgcg cccctgacag aagcccatgc
     1321 ccgcgcgcgt cgcctttttt accgcttcca gcgagtccgc gccgttgcgg atggcttcca
     1381 taatctcgtc tgcggtgact tcctcgcagc ggcagacgat ctccgcgttc attccatcgc
     1441 gttccataac gcctccttgg cggtgcgccg tttttcaccg aacgggccgc acctgagcgc
     1501 gttgagccgt tcgagcagaa cgcacttgtt ctcttcgtac tcttgttcgg aaagatatcc
     1561 cagcgcgtat gccgcgcggc agcccgccat gcggccttcg tccagcgcgg tgctggcttc
     1621 ctcaatgccg gtcaagtcgc ccgcaacgag gatgttttta ctggtcgtct ccatatccgc
     1681 t
//
LOCUS       DAOMGY010000422         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_84738_length_1679_cov_4.194581, whole genome shotgun sequence.
ACCESSION   DAOMGY010000422 DAOMGY010000000
VERSION     DAOMGY010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84738_length_1679_cov_4.194581"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..719)
                     /locus_tag="PLR57_06790"
     CDS             complement(12..719)
                     /locus_tag="PLR57_06790"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQQ41199.1"
                     /translation="MLKNFALKPPFFEIGPKSYLYGDDVLKLALIADAAAKKYDVDVI
                     FTTPVVDIRRVAEATERIYVIAPHMDPLKPGRGLADTLPESLVAAGAKGVMLNHCEKM
                     VSLAALAATIRRADEVGLATIVCTDTSAESAAVARLKPNIIVAEPTDLIGSGKVSDMD
                     YVKKSIQTVKEIDPDIFVLQGAGISSGADVYRVIFAGAEATGSSSGIINAPDRAAMVD
                     EMIGSVRQAWNDRHAKA"
     gene            941..1558
                     /locus_tag="PLR57_06795"
     CDS             941..1558
                     /locus_tag="PLR57_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQQ41200.1"
                     /translation="MTTRKQEEQAWAMATAAYAIEADAITRALEGVKKEDFLRAVDAL
                     KKAPRIGTSGCGHSGIACQHFAHLLCCIERPARFISPAEAIHGATGFLQKGDVMVFAS
                     RGGKTSELLPILKICKEKGVTVISITENLESPLAIGADIVLQMRVTKETDRFNTQGTT
                     STTVLCVLFHALQTALIEVTGFQSEQFAVIHPGGAVGERLNHKSV"
BASE COUNT          372 a          510 c          439 g          358 t
ORIGIN      
        1 tcatttcgcc gtcaggcttt cgcgtgccgg tcgttccatg cctggcgcac agatccgatc
       61 atttcgtcca ccatcgccgc gcggtcgggc gcattgatga tgccgctcga agagccggtc
      121 gcttccgccc ccgcaaagat cacgcggtaa acgtccgccc cgctgctgat gcccgcgcct
      181 tgcagcacga agatatccgg atcgatctcc ttgaccgttt gaatcgactt ttttacatag
      241 tccatatcgc tcaccttgcc ggagccaatc aggtccgtcg gctcggcaac gatgatgttc
      301 ggtttcagcc gggcaaccgc cgcgctctcg gcggaagtat ccgtgcagac aatggtcgcg
      361 agtcccacct cgtccgcgcg gcggatggtc gcggcaagcg ccgcgagcga caccatcttt
      421 tcacagtggt tgagcataac gccttttgcg cccgctgcaa ccagcgactc cggcagcgta
      481 tccgccagcc ctctgccggg tttgaggggg tccatatgcg gcgcgatcac ataaatgcgc
      541 tccgtcgcct ccgccacccg ccgaatatcc actacgggcg tcgtaaaaat aacatccacg
      601 tcgtactttt tcgccgcggc atccgcaatc agcgcaagct tgagcacatc gtctccatag
      661 agatagctct tcgggccgat ctcgaaaaac ggcggcttta acgcaaagtt tttgagcatc
      721 gctcttcctc ctatccgatt gatcgccctt agcttactat tttctttctt tttagtcaat
      781 tttattttct attttttttg ctattgattt acttttgcac aagttttata ctgtttgcgt
      841 agttatatgc ccgtttttga cggacaggac ggaaaaatgg aaaatatgtt ccgctgcggg
      901 cttccccgcg gcatcaacaa aaaaccggag gaatcagcaa atgacaactc gcaaacagga
      961 agaacaggcc tgggcaatgg caacggcggc atacgcaatt gaagcggacg cgattacccg
     1021 cgcgctcgaa ggcgtaaaga aagaagactt tctacgcgcg gtagacgctc tgaaaaaagc
     1081 gccgcgcatc ggcacaagcg gctgcgggca ctccggcatc gcctgccagc atttcgcgca
     1141 tctgctgtgt tgcatcgagc gccccgcgcg gtttatctcc cccgcggaag cgattcacgg
     1201 cgcgacaggc ttcctgcaga agggcgacgt gatggtgttt gcctcccggg gcggaaaaac
     1261 atccgaactc ctgccaattc tcaaaatctg caaggaaaaa ggcgtgaccg tcatctccat
     1321 tacggaaaac ctcgaatctc cgctcgcaat tggcgcggat atcgtgctgc agatgcgggt
     1381 cacaaaggag accgaccgct tcaacacgca gggaaccacc agcaccacgg tgctctgcgt
     1441 actcttccac gcgctgcaga ccgcgctcat cgaagtgacg ggattccaga gcgaacagtt
     1501 cgccgtaatc caccccggcg gcgcggtcgg cgagcggctc aaccacaaga gtgtttagta
     1561 acggggctta aaacccgcta gaaacgtgct ataatagtgt ccgacaacga gcgcgcctac
     1621 cgcgctgtta gctaaccgag gaggccgaac aaccgttatg gcaaacgaac tgaaaattg
//
LOCUS       DAOMGY010000423         1678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_84847_length_1678_cov_2.627234, whole genome shotgun sequence.
ACCESSION   DAOMGY010000423 DAOMGY010000000
VERSION     DAOMGY010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84847_length_1678_cov_2.627234"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1678)
                     /locus_tag="PLR57_06800"
     CDS             complement(<1..>1678)
                     /locus_tag="PLR57_06800"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ41201.1"
                     /translation="NEARALAKFPGDSGVVGVRTFFYENGTAYIVMDFVEGETLKSYA
                     QRRGGRLPAAEVLALFQPILRSLARVHDSGLLHRDISPDNLMLKTDGTLALLDFGAAR
                     QMSIEGEHSNTINVKHGFAPEEQYRTRGEQGPWTDVYALCATIYRLTTGRTPPEALDR
                     MADGTLLTPPNQLGADFTPAQEAAIIHGLAVRASDRTRDVRQLEKELYGGERTVFATP
                     PVSTSYEPAAPNASQSAFVTQPLTVDTKPAAPEPPQSSFVSQPLSVDTEPAKNGVSSK
                     AKNIIGADSEGAQNAAASGGTSPEDPVLQETTPKKANQVRTIATYIAVAAMFILTVFG
                     SGFFGASLRVLLFFADAVCLLWLCFKMGVFKRAKEADKKERTASIAALSGSGLMLVLF
                     LVVIISIYGAPAAAAIDQPAAVATQEPTIVIDWQDPTIEAGIRKALGKSEGEPITTGD
                     VAYITNITIAGDTLYINDDSVWPYVNAYDYTYSVDNGQTSQPVSSQTISLNDLTRFFG
                     LSTLKVYAVNVSGLESLKNCLSLNTVLLDACGDIDLNIFSGFNHLYDLTIY"
BASE COUNT          352 a          464 c          478 g          384 t
ORIGIN      
        1 gtaaatagtt agatcgtata aatgattaaa acccgaaaaa atgttcaggt ctatatcccc
       61 gcaggcgtcc agtaaaaccg tattcaagct gaggcagttc ttcagcgatt ccaggccgga
      121 tacgtttacc gcatacactt tcagtgtaga taaaccgaaa aaccttgtca ggtcattcag
      181 tgaaatcgtc tggcttgaga ccggctgcga agtctgcccg ttgtcaacgg aataggtgta
      241 gtcgtatgca ttaacatacg gccagacgga atcgtcgttg atatacagcg tatcccctgc
      301 gatggtgatg ttggtaatgt atgccacatc ccctgtcgta atcggttcgc cttcgctctt
      361 gcccagcgct ttgcggattc ccgcctcaat ggtgggatcc tgccagtcga taacgattgt
      421 cggttcctgt gtggcaacgg ctgcgggctg atcaatcgcc gcagcagccg gcgcgccgta
      481 aatggaaatg atgacgacca gaaacagcac cagcatcaga ccgctacccg ataaagcggc
      541 aatggaagcg gttctctctt ttttgtcagc ttctttggcg cgcttgaaaa cgcccatttt
      601 aaaacagagc cagagcaggc atacggcgtc tgcaaagaat aaaaggacgc gaagcgacgc
      661 accgaagaag ccggagccaa ataccgttag tatgaacatc gccgcaacgg caatgtaggt
      721 tgcaatggta cgaacctggt ttgccttttt cggcgttgtt tcctgcagta cggggtcttc
      781 gggcgaagta ccgccggatg ccgccgcatt ctgcgcgcct tccgaatcgg ctccgatgat
      841 gtttttcgct tttgagctga ccccgttttt cgcgggttcc gtgtccaccg aaagcggctg
      901 cgatacgaaa gacgactgcg gaggttccgg cgcggcaggc tttgtatcaa ccgtaagcgg
      961 ctgggtcaca aatgctgact gcgaagcatt cggcgcggcg ggttcatagg aagtacttac
     1021 cggcggcgtt gcaaaaaccg ttctctctcc gccgtaaagc tctttctcaa gctgccgcac
     1081 atctcttgtc cggtcggaag cgcgcaccgc caagccatgg ataatcgccg cttcctgcgc
     1141 gggcgtaaag tcagcgccaa gctggttggg gggtgttaat agagtgccgt cggccatgcg
     1201 gtcgagcgct tccggcggcg ttctgcccgt ggtgaggcgg tagatcgttg cgcaaagcgc
     1261 gtaaacgtcc gtccagggcc cttgttcgcc gcgcgtacgg tactgctctt caggcgcgaa
     1321 cccgtgctta acgttgatcg tgttggagtg ctccccttcg atggacatct gccgggccgc
     1381 gccaaagtcg agcagcgcga gagtaccatc cgtttttagc atgaggttgt ccgggctgat
     1441 gtctctgtgc agaagcccgc tgtcgtgcac gcgggcgagc gaacgtaaga tcggctgaaa
     1501 cagcgcgagc acttccgccg caggcagcct gccgcccctg cgctgcgcgt aggatttgag
     1561 cgtttcgcct tcgacgaaat ccatcacgat ataggccgtt ccgttttcgt agaaaaacgt
     1621 tcttacgccc acaacgccgc tgtcgccggg gaattttgca agcgctcttg cctcgttg
//
LOCUS       DAOMGY010000424         1677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_84925_length_1677_cov_3.050555, whole genome shotgun sequence.
ACCESSION   DAOMGY010000424 DAOMGY010000000
VERSION     DAOMGY010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84925_length_1677_cov_3.050555"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1034)
                     /gene="citE"
                     /locus_tag="PLR57_06805"
     CDS             complement(141..1034)
                     /gene="citE"
                     /locus_tag="PLR57_06805"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HQQ41202.1"
                     /translation="MMKLRRTMLFVPGNNPGMMSDAHIYGSDALMFDLEDSVSLGEKD
                     AARLLVYNALKTIHYGDCEQVVRVNPLSTPYGKADIEAMVAAGAEVIRLPKTETAQDI
                     WDTEEEILRAEQKFGVPEGKTRMMAAIEGALGIVNAYAIASASKRLIGIALGAEDYCA
                     NLKTTRSKEGSELFYARSAIVVAARAAGIDAIDTVFSDVNDEEQLVKETQVVKQLGFD
                     GKSVINPRQIKPVHKVFTPTPAEVQKALRIVEAGRQAEARGSGVVSLDGKMIDRPVIL
                     RAERCLELAKASRVMFEGEGK"
     gene            complement(1031..1327)
                     /gene="citD"
                     /locus_tag="PLR57_06810"
     CDS             complement(1031..1327)
                     /gene="citD"
                     /locus_tag="PLR57_06810"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HQQ41203.1"
                     /translation="MQIKGSAAAGTMESGDILIEIEPGEKKEVEVVLKSSVANEFGKQ
                     IVKTIHSAVAAFGLEYAVVNAVDKGALDCTIRARVTAACQRATGETAFDWGREA"
BASE COUNT          320 a          518 c          420 g          419 t
ORIGIN      
        1 gaggctctca acaatcttgt tttcgcgcgc catgcggtcc ttcagcggcg cagcgtcctt
       61 atccatcagg cgcgggtttg cgccctcgat atacggcttt ttgccaagcg cttctacttc
      121 ctgcgcaaaa tcgatctgtt tcatttgcct tccccctcga acatcacacg ggacgccttt
      181 gccagttcca gacagcgttc cgcgcggaga atcacaggcc tgtcgatcat cttgccgtcc
      241 agcgagacga cgccgctgcc gcgcgcttcc gcctgcctgc ccgcttccac gatgcgcagg
      301 gctttctgca cttccgcggg cgtcggcgta aagactttat gaacgggctt gatctgccgc
      361 ggattgatga cgctctttcc gtcgaaaccg agttgcttga ccacctgcgt ctccttgacg
      421 agctgctctt cgtcgttcac atccgaaaaa acggtgtcga tggcgtcgat tcccgccgcg
      481 cgcgccgcaa cgacgatggc cgaacgcgcg tagaacagct cgctgccttc tttggagcgc
      541 gtggttttca ggttggcgca gtagtcctcc gcgccaagcg caatgccgat caggcgcttg
      601 ctcgcgcttg cgatggcgta cgcgttgaca attcccagcg cgccctcgat agctgccatc
      661 atgcgggttt ttccctccgg cacgccaaac ttctgttccg cgcgcagtat ctcttcttcc
      721 gtgtcccaaa tatcctgcgc ggtctccgtt ttcggtaagc gaatgacttc cgcgcccgcc
      781 gcaaccatcg cctcaatatc agccttgcca tagggcgtac tcagcgggtt gacgcggaca
      841 acctgttcgc agtccccgta atgaatcgtt ttaagcgcgt tatagaccaa aagccgcgcc
      901 gcgtcctttt cacccagaga aacggaatcc tccagatcga acatcagcgc gtcgctgccg
      961 taaatatgcg cgtcgctcat catgccgggg ttgttgcccg gcacaaacaa catcgtcctg
     1021 cggagtttca tcatgcttcc cgcccccagt caaacgcagt ttcgcccgtt gcccgctggc
     1081 aggcggcggt aacgcgcgcg cggatcgtgc agtccagcgc gcccttgtcc acggcgttga
     1141 caaccgcgta ttccagcccg aaggcggcaa ccgcgctgtg aatcgtttta acgatttgct
     1201 tgccgaactc gttggcaacc gagcttttca gcacgacttc cacttctttt ttctctccgg
     1261 gttcaatttc tatcaggata tcgccggatt ccatggtgcc cgcggctgcg gagcctttga
     1321 tttgcatatt ggatcagcct ttccgaatcg atcatactct atgcataatc atactagtat
     1381 gcagtcgcct tgacaagaga aaacgcgcga ttgcacagaa tatatacaaa tgtgcagcat
     1441 gcctgtattg ttgtatacac ccacacaatc tctatgtttc tcgtcgtttt gtatttttcg
     1501 cgatctggtt gacgcacatt gttgaaaatg cataacggca tttcgttctg catttttatg
     1561 taatttgcga atctgctttt ctgtcagccg ccgtatggta taatgcggat atggaaatca
     1621 cgctggacga actgctccaa gcgcgcgacg aacgcgcggc atacgaaaaa gagctgc
//
LOCUS       DAOMGY010000425         1674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_85180_length_1674_cov_2.759111, whole genome shotgun sequence.
ACCESSION   DAOMGY010000425 DAOMGY010000000
VERSION     DAOMGY010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85180_length_1674_cov_2.759111"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1674)
                     /locus_tag="PLR57_06815"
     CDS             complement(<1..>1674)
                     /locus_tag="PLR57_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41204.1"
                     /translation="FDHGLTAYEAERLTLYTPEVRPGFTAWTTCFDYGDGRIGLSFKE
                     TLRERDPYYQPPLLEMGEAVGAPVSYCSVECGSELERSFRVYMVSADNGKTFTETGRC
                     PLEEGAFCNFGFPDGRIIGYDVPRINDERTGWFDGIMMRESLDGGTTWHRLGVLLEGC
                     APYLWRARKLKDGTLLLLLSLYGTPWGLGKQRSTRNTMLPGETYINKIQTCFLASKDG
                     VHFSGPHYVLPGIGAHEYDVCECPDGRLLFLAGDVQATPVGRQFVERRGESFINGTLY
                     GVRRGAPADPKHDPMGGFMPESLIMRADGLIIGSRRNKPYSCSADFGENWYEVDGLPN
                     SLYQPFLMEAPDGTILNFGHKGGDMALGQEDMFIGVDRFRVQNGMPASCVLTLMREMD
                     TEQTHYVNAFSALLTAGGKPVTHQAVLFRAHPVWNADGSYSTIPQSEASIQTEAVTDE
                     TGTAKIAFPMFDNRPDIHFYYNIDVVFRPREGMGYSPCDGPMMCVSGMTPHRKNRWPY
                     DAYLAEGRLFLSPALIKAVPDAIKQINALGSRRPVQTGELSEELLSRLI"
BASE COUNT          309 a          528 c          502 g          335 t
ORIGIN      
        1 tgatcagacg gctgagcagt tcctcgctga gttcgcccgt ttgcaccggc ctgcggctgc
       61 ccagcgcgtt gatctgcttt atcgcgtccg gaacggcttt tatcagcgcg ggcgagagga
      121 agagcctgcc ttcagcaagg tacgcgtcgt acggccagcg gtttttgcgg tgcggcgtca
      181 tgccggaaac gcacatcatc ggcccgtcgc agggcgaata ccccatgcct tcgcgcgggc
      241 ggaacaccac gtcgatgttg taataaaagt ggatgtccgg cctgttgtcg aacataggga
      301 acgcaatctt cgccgtgccg gtttcatccg taacggcttc cgtttggatg cttgcctcgc
      361 tctggggaat cgtgctgtaa ctgccgtccg cgttccagac cgggtgcgcg cggaacagca
      421 ccgcctgatg ggtgacgggt ttgccgcccg cggtcagaag cgccgaaaac gcgttgacat
      481 aatgcgtttg ttccgtatcc atttcacgca tgagtgtgag aacgcagctc gccggcatac
      541 cgttctgcac acggaagcgg tcaactccta tgaacatgtc ctcctgcccc agcgccatat
      601 ccccgccctt gtggccgaag ttcaaaatcg ttccgtccgg cgcttccatt aaaaacggct
      661 ggtagaggct gttgggcagg ccgtccacct cgtaccagtt ttcgccgaag tccgcgctgc
      721 aggagtacgg cttgttgcgg cggctgccga tgatcaaccc gtcggcgcgc ataatcaggc
      781 tctccggcat aaagccaccc atggggtcat gcttcgggtc ggcgggcgcg ccgcggcgga
      841 cgccgtagag cgtgccgttg ataaagctct cgcccctgcg ctcgacaaac tgcctgccaa
      901 ccggcgttgc ctgcacatcc cccgcgagaa acagcagccg tccgtccggg cattcgcaga
      961 cgtcgtattc gtgcgcgccg atgcccggca gcacgtaatg cgggccggaa aagtgcacgc
     1021 cgtccttgct cgcaagaaag caggtttgaa ttttattgat gtaggtttca cccggcagca
     1081 tcgtgttgcg cgtagagcgc tgcttgccca gtccccaggg cgtgccgtac aggctcagca
     1141 gaagcagaag tgttccgtcc ttgagcttac gcgcgcgcca gagatacggc gcgcatcctt
     1201 ccagcagaac cccgagccta tgccaggtgg ttccgccgtc gaggctctcg cgcatcatga
     1261 tgccgtcgaa ccagccggtt cgctcgtcgt tgatgcgcgg cacgtcgtag ccgatgatgc
     1321 gcccgtcagg gaagccgaag ttgcagaacg cgccttcctc cagcggacag cgtccagtct
     1381 ccgtgaacgt tttcccgttg tctgcggaga ccatgtagac gcggaacgac cgctccagct
     1441 cgctgccgca ctccacggag caataggaaa ccggcgcgcc gacggcttct cccatctcca
     1501 gaagcggagg ctggtagtag gggtcgcgct cacgcagcgt ttccttaaag gataagccga
     1561 ttctgccgtc gccgtaatcg aagcaagttg tccaggccgt aaaacccggg cgcacttctg
     1621 gcgtataaag cgtcagccgc tcggcctcgt aagcggtcaa accatggtca aact
//
LOCUS       DAOMGY010000426         1672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_85342_length_1672_cov_3.258503, whole genome shotgun sequence.
ACCESSION   DAOMGY010000426 DAOMGY010000000
VERSION     DAOMGY010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85342_length_1672_cov_3.258503"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /gene="rpmB"
                     /locus_tag="PLR57_06820"
     CDS             <1..146
                     /gene="rpmB"
                     /locus_tag="PLR57_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQQ41205.1"
                     /translation="LVSHSNRKTKRGWAPNIQSVHVVVDGKPMKMNVCTRCLRSGKVS
                     RSV"
     gene            470..>1672
                     /locus_tag="PLR57_06825"
     CDS             470..>1672
                     /locus_tag="PLR57_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41206.1"
                     /translation="MEKGAVLRKQRWVFPLCLGFAALFLLLATKSSPLYPLNDWVDAN
                     IYFTIGKGMMHGQVPYLNLYDQKGPTAYLIYGLASLISGTSFLGVYLLEVLAFGGFLY
                     AVYKIVSLFTDKGALLVLPVLCWTILGSLSFSHGGSFEEFMMPLFAFSLYDSLKYFKT
                     IYPKPVPYKTIALNAALAGLMLYGKFTLLAFYIAWMGTIAISQLIVKQWKRALGASAV
                     FVLVMVGVGIPWAVYFSANGALHEFFYYYFYQNIFGYSFLEAPLILTMPYAIVHDVAA
                     FSFRNPQIAGFIALGLIWFVLQKRDQVKTVEKINLILLCALLAAGIYCGGQGYRYYGL
                     VLTPFMALGFVPLIRFWNKSCSDWFSTRALKAGLYGALTLAMLALTFITTDNRYMLLK
                     DKKEMPQTQ"
BASE COUNT          324 a          447 c          452 g          449 t
ORIGIN      
        1 atctggtcag ccactccaac cgcaagacca agcgcggctg ggcgccgaac atccaaagcg
       61 tgcatgttgt cgttgacggg aaaccgatga agatgaacgt ttgcacgcgg tgcctccgtt
      121 ccggcaaggt atcccgcagc gtctaagctg tcaaacttga ttttatgaag aacaccgcgt
      181 attttgcgcg gtgttcttgt ttctgcttta cagaattttt cagctgctgc agcgggagtt
      241 gaaagcgcct tgttttgttg cagagagttc acctttgccg ttttacgtgc gcgttctttg
      301 tttttaagca aaatcgtttg aaacagcgcc gttttaccgc ttgtacgcgc agctttacgc
      361 gcggataccg gtcaggccgc tcgggcatta ttgccaagaa gcgcccgaac cgatataata
      421 agcgagaata tgccggtcta ctgagctttt attgttggag gaaacgggta tggaaaaagg
      481 ggcggttctt cgcaaacaac gttgggtatt tccgctttgc ctgggatttg cggctttgtt
      541 ccttttactc gcaacgaaat catcgccgct gtatccgctc aacgactggg tggacgcaaa
      601 tatctacttt accatcggca agggcatgat gcacgggcag gtgccttacc tgaaccttta
      661 cgatcagaaa ggccctacgg cctatctaat ttacggactg gcttcgctga tctccggcac
      721 gtcgtttctg ggcgtttacc tgctggaagt tctcgcgttc ggcggttttc tctacgccgt
      781 atataagatc gtttcgctct ttaccgacaa aggcgcgctc ctcgtgctgc cggtgctctg
      841 ctggacgatt ctcggtagcc tgagcttttc ccacggcggc agctttgaag agttcatgat
      901 gccgctgttt gcattttcgc tgtacgactc gctgaaatac tttaaaacga tctatcccaa
      961 acccgtcccc tacaaaacga ttgcgctcaa cgctgcgctt gccgggctga tgctctacgg
     1021 caagttcacc ttgcttgcgt tctacatcgc gtggatggga accatcgcca tttcgcagct
     1081 tattgtaaag cagtggaagc gggcgctggg cgcaagcgcg gtgtttgttc tcgtcatggt
     1141 tggcgtaggg attccctggg cggtttactt ttccgccaac ggcgcgctgc acgagttctt
     1201 ctattattac ttctaccaga acatcttcgg ctactccttc ctggaagcgc ccttgattct
     1261 gaccatgccg tacgccattg tgcacgacgt tgcggcgttt tcgttccgca atccgcaaat
     1321 cgcgggattc atcgcgctgg gtttgatctg gttcgtgctc cagaagcggg atcaggtcaa
     1381 gacggtcgaa aagatcaacc tgattctcct ctgcgctctg cttgccgcgg gcatttactg
     1441 cggcgggcag gggtaccgct attacgggct ggtgctcacg ccgtttatgg cgctcgggtt
     1501 cgtgccgctg atccggttct ggaacaagag ctgttccgac tggttttcca cgcgtgcgct
     1561 caaggcgggg ctgtacggcg cgctgacgct tgctatgctc gcgctgacgt ttattaccac
     1621 ggacaaccgc tatatgctct taaaagataa gaaagaaatg ccgcagacgc ag
//
LOCUS       DAOMGY010000427         1672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_85375_length_1672_cov_2.421150, whole genome shotgun sequence.
ACCESSION   DAOMGY010000427 DAOMGY010000000
VERSION     DAOMGY010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85375_length_1672_cov_2.421150"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..430)
                     /gene="rnpA"
                     /locus_tag="PLR57_06830"
     CDS             complement(71..430)
                     /gene="rnpA"
                     /locus_tag="PLR57_06830"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HQQ41207.1"
                     /translation="MKRSDSLRRKKEFRYTYRAGKSCGGRLLSMVYAKNRSAQPKIGF
                     SVSKKIGNSVVRNRVKRRLREALTPLIPSIRSGVNIIFIARDAVVEAPFLTIRESMLS
                     QLDRAGLLKPRETQKDL"
     gene            complement(540..674)
                     /gene="rpmH"
                     /locus_tag="PLR57_06835"
     CDS             complement(540..674)
                     /gene="rpmH"
                     /locus_tag="PLR57_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQQ41208.1"
                     /translation="MFRTYQPKKRQRSKVHGFRKRMKTADGRNVLKRRRAKGRKVLSA
                     "
     gene            complement(866..>1672)
                     /locus_tag="PLR57_06840"
     CDS             complement(866..>1672)
                     /locus_tag="PLR57_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HQQ41209.1"
                     /translation="IGIMIGLLFAFLLSLIVQTINIPVLPEIITILLFFICGYYGGYI
                     GVTRRTELMEGAISRKNRLTGTLSPDGARPKVLDTSVIIDGRICDICRTGVVEGKIIV
                     PAFVLKELRHIADSSDAMKRARGRRGLDILHTMQCEMERRVVVEERDYDDVDEVDLKL
                     LRLAADIGGILVTNDYNLNKVAAVQNMPVLNINELANALRSVLLPGEELSLAIVKEGK
                     EVGQGIGYLPDGTMVIVENGKKRIGETADLVVTSALQTSAGRLVFAKMRS"
BASE COUNT          347 a          477 c          449 g          399 t
ORIGIN      
        1 cgacagcttg gcggcagcat tggagaaatc tgcttttgat aaaaccggat gatcgcgatg
       61 aatacttttt tcataggtct ttctgcgttt cccgtggttt gagtaaaccc gcgcgatcca
      121 gctgtgaaag catcgattcg cgtatggtga gaaacggcgc ttccacaacg gcgtcccggg
      181 cgatgaagat gatgttcacg ccggaacgga tggaaggaat cagcggggtc agcgcttcac
      241 gcagcctgcg cttgacgcgg ttgcgtacaa cgctgttgcc gatttttttg ctgacggaaa
      301 atccaatctt gggctgagca ctgcgatttt ttgcatacac catggaaagc agccgtccgc
      361 cgcaggattt gcccgcgcga taagtatacc gaaattcctt ttttcttcgg agggaatccg
      421 agcgcttcat gtattcgctt cctttttccg attcatttgt aaatacaaac atgcggcaag
      481 caaatgcccg ccgcaagcgc ttgtgcgtac gtgtccggtt tcgatcaacg caatggccgt
      541 tacgcgctca gaactttgcg gcctttggcg cgtctgcgtt tgagaacatt gcggccgtcc
      601 gccgtcttca tgcgtttacg gaagccatgt accttgctcc gctgacgctt tttgggctga
      661 taggtcctaa acatcgattc cacctcgttt cgtattgagt cgcgcaggtt gccccgcgga
      721 aaactatctc tgcatacggg catgttcccg catgataaga accttatgta ttatagagga
      781 cggcgcaaag ccgtgtcaag gagtttcttg cctcccgcct tgcgccgcac acagcaaaag
      841 ctcacgtttt ctatagtaaa cggctctagc tccgcatctt ggcgaacacc agcctgcctg
      901 cggacgtctg caaagcgctt gtgacaacga ggtccgccgt ttcgccaatc cgctttttgc
      961 cgttctccac gattaccatc gtgccgtccg gcaggtagcc gatgccctgc cccacttcct
     1021 tgccttcctt gacgatggca agggaaagct cctcgcccgg cagcaataca gaacgcagcg
     1081 cgtttgccag ctcgttgatg ttgagaaccg gcatattctg cacggcggcg accttattga
     1141 ggttgtagtc gttggtcacg agtatgccgc cgatatccgc ggcaagccga agcagcttga
     1201 gatcgacttc gtccacgtcg tcatagtccc gttcttcaac gaccacgcgg cgctccattt
     1261 cgcactgcat cgtgtgcagg atgtccagcc ccctgcgccc gcgcgcgcgc ttcattgcgt
     1321 ccgaactgtc cgcaatgtgg cgcagttcct tgagcacgaa agcgggaacg ataatcttgc
     1381 cttccacaac gcccgtacgg cagatgtcgc agatgcggcc gtctatgatg acgctcgtat
     1441 cgagcacctt cgggcgcgcg ccgtccggcg aaagcgtgcc ggtcagccgg ttcttgcgcg
     1501 aaatcgcgcc ttccatgagc tccgtacggc gggtcacgcc gatatagccg ccgtaatacc
     1561 cgcagatgaa gaacagaagg attgtgatga tttccggcag gacgggaatg ttgatggttt
     1621 gtacaatcag gcttaaaagg aacgcaaata acagaccgat cataatgccg at
//
LOCUS       DAOMGY010000428         1670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_85498_length_1670_cov_2.932508, whole genome shotgun sequence.
ACCESSION   DAOMGY010000428 DAOMGY010000000
VERSION     DAOMGY010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85498_length_1670_cov_2.932508"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLR57_06845"
     CDS             <1..224
                     /locus_tag="PLR57_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41210.1"
                     /translation="NDAVRLLVCDLIPACDSEDALSAAQVRLWAENATQIQYMDDDYD
                     DKSDGCQFMAYLTKKHDQNILVCVFFANA"
     gene            455..976
                     /locus_tag="PLR57_06850"
     CDS             455..976
                     /locus_tag="PLR57_06850"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HQQ41211.1"
                     /translation="MNLLELLGPTAIPSIICLILGFVLLLIEMFTPGVGAAGVTGVLS
                     LIAVAVMQFGWGNPRVAFYVVAITLLVIILGIIWVVRSLQRGRLSKSFLVLNAQSDGE
                     SVPEVSNAKQSLIGKAGVTITPLRPSGMAEIEGNRVDVLAAGAFIERGKTVVVVKAEG
                     MHILVREETAPAE"
     gene            1054..>1670
                     /locus_tag="PLR57_06855"
     CDS             1054..>1670
                     /locus_tag="PLR57_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007129408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HQQ41212.1"
                     /translation="MGLTIFIIIAAFLFLVLFFSFVPLGLYISARASGVKIGILTLVG
                     MRVRKVVPARIVNPMIKATKAGLEVSINKLEAHFLAGGNVDRVINALIAAQRAGIPLG
                     FEQSCAIDLAGRDVLNAVQMSVNPKVIETPVIAAVAMDGIELRAKARVTVRANIDRLV
                     GGAGEETIIARIGEGIVTTVGSSRSHKDVLENPDRISRTVLDKGL"
BASE COUNT          352 a          483 c          454 g          381 t
ORIGIN      
        1 gcaacgacgc ggtgcgcttg ctggtttgcg atttaattcc cgcatgcgac agcgaggacg
       61 cgctctccgc ggcacaggtt cgtctttggg cggaaaacgc aacccagatt cagtatatgg
      121 acgacgacta cgacgataag agcgacggat gtcagtttat ggcttacctc acaaagaagc
      181 acgatcagaa tatcctcgtc tgtgttttct ttgccaacgc ctgaatcgaa tcagacgata
      241 gcatcgttgc agcaatgttg gtatcgtaat ccgtggatgg ggcgctttca acaaatcgcg
      301 catgtttgcc tgttcctcga agaattttga gaaaacaaca tatgccctgt ctcctgtccg
      361 cgcgctgcgt ataggcggac tcccttttct cgcggcaaaa ttcgtggtat gatgggtatc
      421 atagaaaaag cagattgatc ggaggccatg aataatgaat ctcttggaat tactcggccc
      481 caccgccata cccagcatca tttgcctgat ccttgggttt gtactgctat taatcgaaat
      541 gttcacgccc ggcgtcggcg cggcaggcgt tacaggcgtc ctctcgctga tcgctgttgc
      601 cgtcatgcag tttggctggg gtaacccgcg cgttgcgttt tacgttgtcg ccatcacgct
      661 gctggtcatt atactcggca ttatttgggt cgtacgctcg ctgcagcgcg gcaggctctc
      721 caagtcgttc ctggtgctga acgcgcagtc ggacggcgag tccgtaccgg aagtctcaaa
      781 cgcaaagcag tcgctcatcg gcaaggccgg cgtcaccatc accccgcttc gtccgtcagg
      841 catggcagaa atcgaaggaa accgtgtgga cgtgctggct gccggcgctt tcatcgaacg
      901 cggcaaaacc gtcgtggtcg tcaaggcgga aggcatgcat atcctcgttc gggaagaaac
      961 agctcccgca gaataatccg gacaaattcg gcactggatt cgatcgccgg tttccggcgt
     1021 gatagaactt aaaataacat caggagggtt cttatgggtt tgactatttt tatcattatc
     1081 gcagcgttcc tgtttctcgt tctgtttttc agctttgtac cgcttggact gtatatctcc
     1141 gcccgcgcgt ccggcgtaaa gatcggcatt ctgacgctcg tcggtatgcg cgttcgtaaa
     1201 gtcgttccgg cccgcatcgt taaccccatg atcaaggcca ccaaggccgg tctggaagtc
     1261 agcatcaata agctggaagc gcatttcctt gccggcggca acgtggaccg cgttatcaac
     1321 gcgctcatcg ccgcccagcg cgcaggcatc ccgctgggct ttgaacagag ctgcgccatc
     1381 gaccttgccg gtcgcgacgt gctcaacgcc gttcagatga gcgttaaccc gaaagtcatt
     1441 gaaacccccg tcatcgccgc ggttgccatg gacggcatcg aactgcgagc caaagcacgc
     1501 gttaccgtcc gcgccaacat cgaccgcctc gtcggcggcg caggcgaaga aacgatcatc
     1561 gcccgtatcg gcgaaggcat cgtcaccacc gtcggttcca gccggagcca caaggacgtt
     1621 ctggagaacc ccgaccgcat ctcccgcacc gttctggata agggcctcga
//
LOCUS       DAOMGY010000429         1668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_85648_length_1668_cov_2.843149, whole genome shotgun sequence.
ACCESSION   DAOMGY010000429 DAOMGY010000000
VERSION     DAOMGY010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85648_length_1668_cov_2.843149"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /gene="asnS"
                     /locus_tag="PLR57_06860"
     CDS             <1..1144
                     /gene="asnS"
                     /locus_tag="PLR57_06860"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HQQ41213.1"
                     /translation="PLVLVSDFVGHEIVLEGACPADYPLQKKRHTLEYLRTIQHLRPR
                     TNTFQATFRVRSVVAAAIHEFFQQNGFVYVHTPLITGSDCEGAGEMFQVTTLPLKDMP
                     KDKDGNVDFSGDFFGKPANLTVSGQLYGEAFAMAFRDIYTFGPTFRAEYSFTARHAAE
                     FWMIEPEMAFCDLAGDMDIAEAMIKYIIKTVLDRCPAEMEFFNQFIDKGLLERLQNVL
                     ENDFVRLSYTKAVELLKASGAEFKYPVEWGLELQTEHERYLTEQVYHAPVFVTDYPKD
                     FKAFYMRLNDDGKTVAASDLLVPGVGEIIGGSQREERLDMLEKRIEELKMDISQYSWY
                     LELRKFGGVKHAGFGLGFERMVMYVTGMQNIRDVLPFPRTTANMEM"
     gene            1226..>1668
                     /locus_tag="PLR57_06865"
     CDS             1226..>1668
                     /locus_tag="PLR57_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41214.1"
                     /translation="MLKKLTKEDAAYTAERCWALYQDPASRCYPVHESRETMARSFAV
                     FCEYESGTLLGYWSGDALTGVCELYFEQEIRYLRITALYAWDDVCAALDAFLNEIDSS
                     YAGYEVNVGLPPENARLAAALAKHGYAVAEENFDLRYDAVSLTPN"
BASE COUNT          340 a          474 c          517 g          337 t
ORIGIN      
        1 gccgctggtg ctggtctccg acttcgtcgg ccacgagatc gtgctggagg gcgcatgccc
       61 cgcggactac ccgctgcaaa agaaacggca tacgctggaa tacctgcgca cgattcagca
      121 ccttcgcccg cggacgaaca cctttcaggc aacgtttcgc gtgcgctccg tggtggcggc
      181 cgccattcac gagtttttcc agcaaaacgg gtttgtctat gtgcataccc cgctgatcac
      241 cggctcggac tgcgaaggcg caggcgagat gtttcaggtc acgacgctgc cgctgaagga
      301 catgccgaag gacaaggacg gcaacgtcga tttctccggt gatttcttcg gcaagcccgc
      361 gaacctgacg gtttccggcc agctctacgg cgaggcgttt gcgatggcgt tccgggatat
      421 ctacaccttc gggcccacgt tccgcgcgga atacagcttc acggcgcgcc acgcggcgga
      481 gttctggatg atcgagccgg agatggcgtt ctgcgatctc gcgggggata tggacatcgc
      541 cgaagcgatg atcaagtaca tcatcaagac cgtgctcgac cgctgcccgg cggagatgga
      601 gttctttaac cagtttatcg acaaaggcct gctggagcgg ctccagaatg tgctcgaaaa
      661 cgacttcgtc cggctgagct acaccaaggc cgtcgaactg ctcaaagcct ccggcgcgga
      721 gtttaaatat cccgtggagt ggggtctgga actgcagacc gagcacgagc gttacctcac
      781 cgagcaggtg taccatgcgc cggtgttcgt taccgactac cccaaggact tcaaagcgtt
      841 ttatatgcgc ctcaacgacg acggcaagac cgttgccgct tccgatttgc tggtgccggg
      901 cgttggcgaa atcatcggcg gcagccagcg tgaagagcgg ctggatatgc tcgaaaagcg
      961 cattgaagaa ctcaagatgg atatcagcca gtacagctgg tatctcgagc tgcgcaaatt
     1021 cggcggcgtg aagcacgcgg gcttcgggct cggctttgag cgcatggtca tgtacgtcac
     1081 cggcatgcag aacatccggg acgtgctccc cttcccgcgc acgaccgcga acatggagat
     1141 gtaatttact tgcattgcca cagcgcgccg gcggagcagt tctgccggcg cgtttttatc
     1201 agtgaatttt ttatgggaga ccgcgatgct caagaaactg acaaaagaag acgccgccta
     1261 tacggcggag cgctgctggg cgctctatca ggatccggcg tcgcgctgtt acccggttca
     1321 tgaaagccgg gaaaccatgg cgcgttcttt tgccgttttc tgcgaatacg agagcggaac
     1381 gcttttgggg tactggtcag gagacgcgct tacgggcgtc tgtgagctgt atttcgagca
     1441 ggagatacgt tatctgcgga taaccgcgct ttacgcctgg gacgacgtct gcgcggcgct
     1501 ggatgcgttt cttaacgaga tcgacagcag ctacgccggg tatgaagtga acgttggctt
     1561 accgccggag aacgcccgtc tggcggccgc gctggcaaag cacggctacg ctgtggcgga
     1621 ggagaacttc gacctgcgat acgacgcggt gtcgcttaca ccgaacga
//
LOCUS       DAOMGY010000430         1667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_85748_length_1667_cov_2.862903, whole genome shotgun sequence.
ACCESSION   DAOMGY010000430 DAOMGY010000000
VERSION     DAOMGY010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85748_length_1667_cov_2.862903"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PLR57_06870"
     CDS             complement(<1..871)
                     /locus_tag="PLR57_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HQQ41215.1"
                     /translation="MESCVQILSREFNKNETHVQNVVSLIDEGYTIPFIARYRKEMHG
                     TMDDTVLRDLADRLTYLRNLAERREEVKKAITAQDKLTPELEKQIDEAKTLAEVEDIY
                     RPYKQKRRTRATIAKEKGLEPLALVLLEQKKDLPPLETLAEAYVDAEKGVASVEEALA
                     GASDIIAEIVSDNAEIRRRLRDNIAKGAKVGSEAAKEEDSVYSLYYKFEQPLSKMQGH
                     QILAINRGEKEGLLKVSLEVERDLALGVVTQAVVKPGSRATEFVRAAAADAYDRLILP
                     SMERETRGTMTDMA"
     gene            1133..>1667
                     /locus_tag="PLR57_06875"
     CDS             1133..>1667
                     /locus_tag="PLR57_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HQQ41216.1"
                     /translation="MKSASHHAMGYLLITALEQEGVQLDKEQFVLGNLLPDYLPELIL
                     APHFTMKCQREIGLFTGVLAAQRVAAKAELPADYSLRLGILCHYITDYFCFAHSREFQ
                     QNLAHHSSYEQALDSYFRDHYTTEECLLPVAAGLRAASAEEAAQEIFRVKRDYKNAER
                     CFQTDVQYAFSVCLGAIH"
BASE COUNT          329 a          497 c          442 g          399 t
ORIGIN      
        1 aggccatgtc cgtcatcgta ccgcgcgttt cgcgctccat ggagggcaga atcaggcggt
       61 cgtaggcatc cgcagcggcc gcgcggacaa actccgttgc gcgggagccg ggcttgacca
      121 ccgcctgggt cacgacgccc agcgcaaggt cgcgctccac ttccagagaa accttcagca
      181 agccttcctt ttccccgcgg ttgatggcga gaatttggtg accctgcatt ttggaaagcg
      241 gctgctcgaa tttgtaatac aagctgtaga cggaatcctc ttcctttgcg gcttcggaac
      301 cgaccttcgc gcctttggcg atgttgtcgc ggagcctgcg gcgaatttcc gcgttgtccg
      361 aaacgatttc cgcaatgatg tcggaagcgc ccgccagcgc ttcttccacg ctggcaacgc
      421 ctttttccgc gtccacatac gcttccgcca gcgtttccag cggcggcaga tccttctttt
      481 gttccagcag gacaagcgcc agcggctcca gtcccttttc ctttgcgatt gtcgcgcgcg
      541 tgcggcgttt ctgcttatac ggccggtaga tatcctccac ctcggcgagg gtctttgctt
      601 cgtcgatctg cttttccagt tcgggcgtga gcttgtcctg cgccgtgatc gcctttttta
      661 cttcctcgcg ccgctcggcg agattgcgca ggtaggtcag cctgtcggcc aggtcgcgca
      721 ggacggtatc atccatcgtt ccgtgcatct ccttgcggta acgcgcgatg aacggtatgg
      781 tgtacccctc gtcgatcaaa gaaacgacgt tctgcacgtg cgtttcgttc ttattgaatt
      841 cacgggataa aatctgtacg cagctttcca tctgttctcc ttgacggtct gtttttccgt
      901 tctccacccg gtttctcaac gggcgtacgg ctattttacc ataaatccgc ccgcgcggta
      961 cagccacttg acggcaggtc aaagcgcggc gcatgccgca ttctgcaaaa aacagccaaa
     1021 taaagcctgt ttttgcaaaa attttaatgt ctccttaatc tgtttttgat cttccggcgt
     1081 tatgataagg acgataaaga agaaaagtcg agaaaagaaa aaggagagaa gaatgaagtc
     1141 tgcttcgcat catgcgatgg gttacctgct gataacggca ctggaacagg aaggcgttca
     1201 actggataaa gaacaatttg tgctcggtaa cctgctgccg gattacttgc cggagctcat
     1261 tttagccccg cattttacaa tgaagtgtca gcgggaaatc ggcctgttta ccggcgtgct
     1321 cgccgcgcag cgcgttgccg cgaaagcgga actcccggcg gactactccc tgcggctggg
     1381 catcctctgc cactatatta cggattactt ctgctttgcg cattcgcgcg agttccagca
     1441 gaaccttgcg caccactcca gctatgagca ggcgctggac agctatttcc gcgaccacta
     1501 caccacggaa gaatgcctgc ttcccgtcgc cgcaggtttg cgcgccgcca gcgcagaaga
     1561 agccgcgcag gaaatcttcc gcgtcaagcg cgactacaaa aacgccgaac gctgcttcca
     1621 aacggacgtg cagtacgcct tctccgtctg ccttggcgca atccatg
//
LOCUS       DAOMGY010000431         1656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_86636_length_1656_cov_3.020612, whole genome shotgun sequence.
ACCESSION   DAOMGY010000431 DAOMGY010000000
VERSION     DAOMGY010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86636_length_1656_cov_3.020612"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..51)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(150..>1656)
                     /locus_tag="PLR57_06880"
     CDS             complement(150..>1656)
                     /locus_tag="PLR57_06880"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HQQ41217.1"
                     /translation="EAAQSAYELHVSQHLYNWSVDFTGAFTLAYTSVGNDAAKNDLLE
                     SISTSVLIAYAPATPTPLPTPEPTETPQPTVTPAPEEATAAPAISGGEVTDKISGLAE
                     RFGTAAIIAGAGLVLSVLMALLGYRKLRQKFRRGDVICFCLIPLAVGLTVCALLSGMQ
                     ESKENAAALEEARIAAEATPEPTPAATPEPTPTATPEATEPPVETVAETSEENFYRRE
                     GEPEEVILVDEENGRWEYRTDELGIVIDRVSTTNAEGKPLTYFVADIHMKNIYQFRAG
                     FGSEGHTGRGAIYPWLIARRAKAVLWITGDNLINSEKSDKGVLIRDGRIYSDNQAEDT
                     LALYPDMTMHIYGEWEMRARDLLEDGVENSFSFGPTLINNGVVNEDAKYHRVRRINPR
                     TGIGYLGPGHFICIVVDGRQKDYSVGMTIWEFADLFVQYNCSIAYNLDGGLSAGMIFM
                     GEQLNSHSGQRIGEKNDISYQRAVPDGLMFGYSDQVPSENDPIANNGNKSK"
BASE COUNT          315 a          494 c          482 g          365 t
ORIGIN      
        1 acaggttgcc gggcttcaca gggccggtcc ctcagccact cttgataagg cggtatgcag
       61 ttttttgttc gaaccgtatt gtagcacagg cggtgcgtct tcgtcaataa cagaaaaggg
      121 tgtgtgagca agctcccaca cccttttctt tatttactct tattaccatt attcgcaatg
      181 gggtcgtttt cgctcggcac ctggtcggaa tagccgaaca tcagcccgtc cggcacggca
      241 cgctgatacg aaatatcgtt cttttcgccg atgcgctggc cggaatgcga gttgagctgc
      301 tcgcccataa agatcatgcc cgcgctaagc ccaccgtcga ggttataggc gatggagcag
      361 ttgtactgga caaacaaatc cgcaaactcc caaatcgtca tgccgacgct gtagtctttt
      421 tgccgtccgt ccacaacgat gcagatgaaa tgcccggggc cgagataacc gatacccgta
      481 cgcgggttga tgcgacgaac gcgatgatac tttgcgtcct cgttgaccac gccgttgttg
      541 atgagcgtcg gcccgaaaga aaacgagttc tccacgccgt cctccagcag gtctctggcg
      601 cgcatctccc actcgccgta gatatgcatc gtcatatcgg gatacagcgc aagggtgtcc
      661 tccgcctgat tatccgaata aatgcgcccg tcgcggatca atacgccctt gtcgctcttt
      721 tcgctgttga tcaggttgtc gcccgtaatc caaagaacag ccttagcgcg ccgcgcgatc
      781 agccaggggt agatcgcccc gcgcccggta tgcccttccg agccgaaacc cgcgcggaac
      841 tgatagatgt ttttcatgtg gatatccgca acaaagtacg tcagcggctt tccttccgcg
      901 ttggtcgtcg aaacgcggtc gatgacaatg cccagctcgt ccgtacggta ttcccagcgg
      961 ccgttttctt cgtccaccag aatcacttct tccggctcgc cttcgcggcg gtagaagttc
     1021 tcttcggaag tttcggcaac ggtttctacg ggcggttcgg ttgcttccgg cgtggcggtc
     1081 ggcgtcggtt ccggggttgc ggccggggtg ggttcgggcg tggcttccgc cgcgatgcgc
     1141 gcttcttcca gcgccgcggc gttctctttg gactcctgca tgcccgaaag cagcgcgcaa
     1201 acggtgaggc cgaccgcgag cggaatcagg cagaagcaga tgacgtcgcc ccggcggaac
     1261 ttctggcgaa gtttgcggta gcctaagagc gccatcagaa ccgacagcac aagcccagcg
     1321 ccggcgataa tcgccgccgt gccaaatcgt tcggccaatc cggaaatctt atcggtgact
     1381 tccccgcccg aaatcgcagg agcggctgtc gcctcctcgg gcgcgggcgt aaccgtcggc
     1441 tgcggcgttt cagtcggttc gggcgtaggc agtggcgtag gcgttgcggg cgcgtacgca
     1501 atcagcacgg aagttgagat gctctcgagc aggtcgttct ttgccgcgtc gttgccgacg
     1561 gacgtatacg ccaacgtaaa cgcgccggtg aaatcgacgc tccaattgta aaggtgctgc
     1621 gaaacgtgca gttcatacgc ggattgcgcg gcttcc
//
LOCUS       DAOMGY010000432         1649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_87171_length_1649_cov_3.083438, whole genome shotgun sequence.
ACCESSION   DAOMGY010000432 DAOMGY010000000
VERSION     DAOMGY010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87171_length_1649_cov_3.083438"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PLR57_06885"
     CDS             complement(<1..1052)
                     /locus_tag="PLR57_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQQ41218.1"
                     /translation="MSKIELQHIDKFYGANHVLRDINLTIEDGAFMTLLGPSGCGKTT
                     TLRVISGLEKPQNGVMLIDGNVMIDAKESFFEAPSKRNLNLVFQSYALWPHMTTFENV
                     AFGLKIKHIPRHEVEERVMSALRKMRIEEYRDRYPNELSGGQQQRVAIARAIATSPKL
                     LLLDEPLSNLDARLRIDMRTELKRLHRETGTTIIYVTHDQVEALTMSTKIALFKEGEI
                     AQVATPLELYTNPIDLAAADFIGNPRINFIDAVGFFNGSELRVKSELGEYRFVKEKMK
                     PGEEYPENQEFDCVLGIRPEMVEIETESTGRDHSVAATVYASQPAGSETLVTLTVGKA
                     EFLAIQIGLTEYDINQ"
     gene            complement(1098..>1649)
                     /locus_tag="PLR57_06890"
     CDS             complement(1098..>1649)
                     /locus_tag="PLR57_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQQ41219.1"
                     /translation="SRGRGKWYGKLTEQLVFIPYLMPGVAFSAIYFAMFSQPRLGGII
                     PSLYGTFMLILLTSIVKHFPFASRSGSANMMQISVELEEAADIAGASFWRRIRSIVIP
                     LAKNGFISGFMLVFISIAKELDLIAILMTPANYTLSFLAFEYSKSLMPQCADAIAIVI
                     LLFILVCYRVANLFGADIGKSWG"
BASE COUNT          345 a          439 c          485 g          380 t
ORIGIN      
        1 ttctggttga tgtcgtattc ggtcaggccg atctgaatgg cgaggaactc cgccttgccg
       61 accgtaaggg taaccagtgt ttcgctgccc gcgggctggc ttgcataaac ggttgccgca
      121 acggaatggt cgcgtccggt gctctcggtt tcgatttcaa ccatttcggg acggatgccg
      181 agcacgcagt caaactcctg attttccgga tactcttcac ccggcttcat cttctctttg
      241 acaaagcggt actcgccgag ctcgctctta acgcgcagtt cgctgccgtt gaagaagcct
      301 accgcgtcga taaagttgat gcgcgggttg ccgataaaat ccgccgcggc aaggtcgatg
      361 gggttagtgt agagctccag cggcgttgcg acctgcgcga tttcgccttc tttaaagagg
      421 gcgatcttcg tggacatggt cagcgcttcc acctggtcgt gggtgacgta aataatcgtc
      481 gtgccggtct cgcggtggag acgcttgagc tcggtgcgca tgtcgattct gagacgtgcg
      541 tcgaggttgg agagcggctc gtccagcagc agcagtttcg ggctggtggc gatggcgcgc
      601 gcgatggcga cgcgctgctg ctgaccgccg gaaagttcgt tggggtaacg gtcgcggtat
      661 tcttcaatgc gcattttgcg cagggcgctc atgacccgtt cctctacttc gtgacgcggg
      721 atgtgcttga ttttgagccc gaacgctacg ttttcaaacg tggtcatgtg cggccagagc
      781 gcgtagctct ggaacacgag gttcaggttg cgcttgctgg gcgcttcaaa gaaggattct
      841 ttcgcgtcga tcatcacgtt gccgtctatg agcatcacgc cgttctgggg tttttccaat
      901 ccggaaatca cgcgcagcgt ggtggttttg ccgcagccgg agggaccgag cagggtcata
      961 aatgccccgt cttcaatcgt gaggttgata tcgcgcagaa cgtgattcgc gccgtaaaac
     1021 ttatcgatat gctgtaattc aatcttactc attgcagata accctttctc tctttacaaa
     1081 tgctgcgcaa aggtcgctta accccagctc ttgccgatat cggcgccaaa gaggttcgca
     1141 acgcggtagc aaaccagaat aaacagcaga atcacaatcg caatcgcgtc tgcgcactgc
     1201 ggcattaggc tcttggaata ctcgaaagcg aggaacgaaa gcgtgtaatt cgcgggcgtc
     1261 atcaaaatgg cgatcaggtc gagttccttg gctatgctga taaacaccag catgaacccg
     1321 gagatgaacc cgtttttcgc cagcgggatg acaatgctgc gaatgcgccg ccagaagctg
     1381 gctccggcaa tatccgccgc ctcctccagc tcaacgctga tctgcatcat gtttgccgag
     1441 ccggaccggc ttgcaaacgg gaagtgcttg acgatggagg tcagcagtat cagcatgaac
     1501 gttccataga gcgaaggaat aatgccgccc aatcgaggct gcgagaacat tgcaaagtag
     1561 attgcggaaa acgcaacgcc tggcatcagg tagggaatga acacgagctg ctcggtgagt
     1621 ttgccgtacc atttgccgcg cccgcggga
//
LOCUS       DAOMGY010000433         1648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_87236_length_1648_cov_3.180163, whole genome shotgun sequence.
ACCESSION   DAOMGY010000433 DAOMGY010000000
VERSION     DAOMGY010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87236_length_1648_cov_3.180163"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLR57_06895"
     CDS             <1..193
                     /locus_tag="PLR57_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQQ41220.1"
                     /translation="SSLKAGDRVRTIGGIYGTIGTIKDDTVILLVGPDKVKLVFARGA
                     IAQVEEAGVENEMTQEVMK"
     gene            404..658
                     /locus_tag="PLR57_06900"
     CDS             404..658
                     /locus_tag="PLR57_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HQQ41221.1"
                     /translation="MQDNLRDTQQFEISREKRSAKDTLVSVYEAMREKGYDPVNQIVG
                     YLLSGDPTYITSYNNARYLISRIERDELLEELVRAYVERK"
     gene            670..1089
                     /gene="ruvX"
                     /locus_tag="PLR57_06905"
     CDS             670..1089
                     /gene="ruvX"
                     /locus_tag="PLR57_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQQ41222.1"
                     /translation="MRILAFDIGERRIGVAVSDPLGITAQGIETYTRTDDLQKDVAYL
                     LSLANGYRPVKLVFGMPRNMDGSYGMQAEYTKAFAEEVLKGWDGDSAFWDERLTTASA
                     RRVLIEADMRRDKRKGVIDKVAAVLILQSYLDAKGNT"
     gene            1149..1457
                     /locus_tag="PLR57_06910"
     CDS             1149..1457
                     /locus_tag="PLR57_06910"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HQQ41223.1"
                     /translation="MEEPGNIVELVDESGKTVAFDLVMTFDYEGKRYAALLPVDQVDG
                     VEDDEVVLLEIVKDKSGENYVTIENPILLDEVFQEFLDLFDEMIDEDEDAQDEEDKEE
                     "
BASE COUNT          461 a          400 c          453 g          334 t
ORIGIN      
        1 ttcctcgctg aaagccggcg accgcgtacg caccatcggc ggcatctatg gcacgatcgg
       61 cacgatcaag gacgacaccg tcattctgct ggttggtccg gataaagtta aactggtctt
      121 tgcccgcggc gctattgcgc aggtggagga agcaggcgtt gaaaatgaaa tgacgcagga
      181 agtcatgaaa taacgctttc atcagccatt tctacgctcc gcacggcaca gccgcgcgga
      241 gcgttttgtt tcaccaaaca tgtttgtaaa acttttggtt ctccaagttc gtgaataacg
      301 agaaaacgga atgtgaacga acggcaaatt cggatcaggt tggtaaaaac gctatccatt
      361 cgagctcatt tttggtataa ttttctccag agaggtgaac tccatgcaag acaatctcag
      421 agatacccaa caattcgaaa tttcgcgcga aaagcgcagc gcaaaggaca cgctcgtgag
      481 cgtttacgaa gccatgcgcg aaaagggcta cgatccggtc aaccagatcg taggctatct
      541 tctttccggc gacccgacct atatcaccag ttataacaac gcccgctatc tgatttcccg
      601 catcgaacgg gacgaactgc tcgaagagct cgtccgcgcg tacgtggaaa ggaaataacg
      661 gaagaaatgc tgagaatact ggcgtttgat atcggagaaa ggcgcatcgg cgttgcggtc
      721 agcgatcctc tgggcattac cgctcagggc attgaaacct atacccgcac ggacgacctg
      781 caaaaagacg ttgcttacct tttgtccctt gccaatggat acagacccgt caagcttgtg
      841 ttcggcatgc cgcgcaatat ggacggaagc tacggcatgc aggcggagta cacaaaggcc
      901 tttgccgagg aagtgctcaa gggctgggac ggcgactccg ctttctggga cgagcgcctc
      961 actaccgcca gcgcgcggcg cgtgctcatt gaagcagata tgcggcgcga caaacgcaag
     1021 ggcgtcatcg ataaagtagc ggcggtactg attctgcaaa gctacttgga cgcaaagggc
     1081 aacacctgat cgggaaaacc ctgaaaaaaa tcccaacgcc gccttaaatg cgcgggaaag
     1141 gaaaagagat ggaagaacca ggcaatatcg tagagcttgt agacgaaagc ggaaagacgg
     1201 ttgcgttcga ccttgtgatg acgtttgact acgaaggcaa gcgttatgcc gctctgcttc
     1261 cggtcgacca ggtggacggc gttgaggacg acgaagttgt gctgctggag atcgtaaaag
     1321 ataaaagcgg cgaaaactac gttaccattg aaaacccgat cctgctggac gaagtctttc
     1381 aggaatttct cgacctgttc gacgagatga tcgacgagga cgaggatgca caagacgaag
     1441 aagataagga agagtaacgt tcttctgatg ctgaaaaagc cggattttcc gcataaaatc
     1501 tgaaataatg cttgataaaa gaaaatacaa cgtgctataa tacgcgaggt atcacgcacc
     1561 ggtgcggatt ccgcggctcg tgccccgaac aatcggggtt accgggggag cagaaacacc
     1621 gggaagatta aaacgaggag gttttact
//
LOCUS       DAOMGY010000434         1647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_87344_length_1647_cov_2.799623, whole genome shotgun sequence.
ACCESSION   DAOMGY010000434 DAOMGY010000000
VERSION     DAOMGY010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87344_length_1647_cov_2.799623"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLR57_06915"
     CDS             <1..530
                     /locus_tag="PLR57_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41224.1"
                     /translation="HSDERAQMRRPTFVCAYMALTSAFSIVTLLAMEQTSQTIELPLS
                     GGRTLRVYSDKAQAERCADNSVIAVYDDRHTGNPSFQIENSCRITRRRERLEVLKALI
                     AFDREHPADPPWHRSLHSLEREWAEHNLAYRVGFLRSRARSVDLDNHAEGKGAFGYFL
                     QSAWGVIKGYLKPKK"
     gene            complement(616..1401)
                     /locus_tag="PLR57_06920"
     CDS             complement(616..1401)
                     /locus_tag="PLR57_06920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQQ41225.1"
                     /translation="MTQKPLQEHPLRHLLWLTVPLITAVLFVAVTLISSAAYDLASNV
                     IVPVSHWDPDQCFLLISVHHVFQAVIAFVVILLLSLLLRKSLSEFGFNRNAFRYSVRS
                     VLIFSGAWFVIQMAASILVPLFTGLDASLAYPLTLRNFTGNFLFEILLSGTSEEILFR
                     SMTIPLILAFFRKFTKRDSLAWGVAIAVATLVFTLAHINFNLNPFTITHLNVAQLVTC
                     VIFGLYYGLLMKKTGSILGPMLAHNLLNGVITISALIMTLWGA"
BASE COUNT          425 a          431 c          481 g          310 t
ORIGIN      
        1 ggcattcaga cgaacgcgca caaatgcgca ggccgacttt cgtttgcgca tatatggcgt
       61 tgacaagcgc gttttcgatt gttacgctgt tggctatgga acaaacgagt caaacaatcg
      121 aacttccgct ctccggcggc agaacgctcc gcgtctacag cgacaaggcg caggcggaac
      181 gatgcgcgga caacagcgtc atcgccgtat acgacgaccg ccataccggc aacccgtcgt
      241 ttcaaattga gaactcctgc cgcatcaccc ggcgcaggga acggctggag gtgctcaaag
      301 cgctgattgc gttcgaccgc gaacaccccg ccgacccgcc ctggcacaga tcgctccatt
      361 ctctggagcg cgaatgggcg gagcataacc tcgcctaccg ggtggggttt ttgcgcagcc
      421 gcgcgcggag cgtcgatctg gataaccatg ccgagggcaa gggcgcgttc gggtactttc
      481 tccagagcgc gtggggcgtg atcaaggggt atctcaagcc aaagaagtag acgaaaagaa
      541 gatgcgaaga aacacaagcg gcttccgcag gacggaaagc cgcttttttc gtttatagaa
      601 tactcgaaat cgaatttacg cgccccagag cgtcatgatc agcgcggaaa tcgttataac
      661 cccgttgagc aggttatgcg ccagcatggg cccgaggatg ctccctgttt tcttcatcag
      721 cagaccgtaa tacaaaccga atatgacaca ggtgacgagc tgcgcgacgt tcaggtgcgt
      781 tatggtaaac gggttgaggt tgaagttgat atgcgccagc gtaaacacca gcgtcgcaac
      841 cgcaatcgcc acgccccagg ccagcgaatc gcgcttggta aacttgcgaa agaaggcgag
      901 tatgagcggg atggtcatgg aacgaaacag gatttcctcg ctggtgccgg aaagcaggat
      961 ttcgaaaaga aaattgccgg tgaagtttcg caaggtcagc gggtacgcaa gagacgcgtc
     1021 cagcccggta aagagcggca caagtatgct tgcggccatt tggatgacaa accaggcgcc
     1081 ggagaagatg agcacgctgc gcaccgagta ccggaacgca ttgcggttga aaccgaattc
     1141 cgacaggctt tttcgaagca gcagcgacag caggaggatg accacaaatg cgatgaccgc
     1201 ctgaaataca tgatgaacgg agattaatag aaaacattga tcgggatccc aatgcgaaac
     1261 aggcacgatg acgttgctgg cgaggtcgta cgccgcggaa ctgatcagcg ttaccgcaac
     1321 gaacagaacg gcggtaatca gcggcaccgt gagccagagt agatggcgca ggggatgctc
     1381 ctgcaggggt ttttgcgtca taagggattc tcctgatgaa tgatattttg cagataaaaa
     1441 aatacaccgt ctgatcgaca gtgtaaagag atacttgcat ttgtgaccga aatgtaagaa
     1501 gaagggtgca gtgcgttcaa tccgcccggt tacagcccca acgattcgtg gacttcccgc
     1561 atgccgagga tggattcctg aatcacttct tccagcgtca tgccgagcat gtcgcagccg
     1621 ccaaggatga cgtcccggtt cacgccc
//
LOCUS       DAOMGY010000435         1643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_87667_length_1643_cov_3.255038, whole genome shotgun sequence.
ACCESSION   DAOMGY010000435 DAOMGY010000000
VERSION     DAOMGY010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87667_length_1643_cov_3.255038"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLR57_06925"
     CDS             complement(<1..558)
                     /locus_tag="PLR57_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006770577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQQ41226.1"
                     /translation="MKELSLNILDIAQNSIKAGAARVEISLEQSGAWLTITISDDGSG
                     MSPEFAAAAADPFTTTRTTRKVGMGLPLFKLAAEQTGGAFSIVSHQAAFEGDRHGTTV
                     RAAFDTSHVDCEPLGDVASTVTTLIQGNPELELIYRYRTEAGQKVLSTEEMRQMLGDE
                     VPLNSPEVLAWAKAFLEDGELEPFDP"
     gene            complement(617..1354)
                     /locus_tag="PLR57_06930"
     CDS             complement(617..1354)
                     /locus_tag="PLR57_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQQ41227.1"
                     /translation="MSGFRCDLHIHSCLSPCADDDMTPYSIAGMAKLNGLAVAALTDH
                     NACGNCESFFQACRGYGIVPVAGMELTTSEDVHLLCLFRTLEDAKAFSAEVENMRIRI
                     PNREKVFGNQLLIGLDDEPTGREPYLLINATLLSLEEAAQLASIHGGAAIPAHVDREG
                     NGVIAVLGDLPESPAFAAVEYRELTNASAYEAQYPRLRGKRKIVSSDAHRLWEIASPG
                     CLLELNCPADAKEQQVRDALIDFLLGK"
     gene            complement(1351..>1643)
                     /locus_tag="PLR57_06935"
     CDS             complement(1351..>1643)
                     /locus_tag="PLR57_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41228.1"
                     /translation="EPFVVSDGERKITGGYAGDLLSWVMGRANEGDAWVTIMSNPNIV
                     AVATLADVACIVLAEGVAPDAGVTEIALARGINLLGSGESAFALCGRIAGLI"
BASE COUNT          301 a          543 c          473 g          326 t
ORIGIN      
        1 cggatcaaag ggctccagtt cgccgtcctc gagaaacgct tttgcccagg cgagcacttc
       61 cggcgaattc agcggaactt cgtctcccag catctggcgc atctcctccg tggagagcac
      121 tttctgcccc gcttccgtgc ggtaccggta gatcagctcg agttccggat tgccctgtat
      181 cagggttgtt accgtggaag cgacgtctcc gagcggttcg cagtccacat ggcttgtgtc
      241 gaacgcggcg cgcacggttg ttccgtgcct gtccccctca aacgccgcct gatgggacac
      301 aatggagaac gctccccccg tctgctccgc cgccagcttg aacagcggca gtcccatgcc
      361 gacctttctg gtcgttcggg tcgtggtaaa cgggtcggca gccgccgcgg cgaattccgg
      421 gctcatgccg cttccgtcgt cggagatggt tatcgtgagc cacgctcccg actgctccag
      481 cgaaatctca acgcgcgccg ctcccgcctt gatggagttc tgcgcaatat ccagaatgtt
      541 gagcgaaagt tccttcatat aaatatcctt ccgttcaaac ccgtcgggac agaccgtcgg
      601 gacgcattcg ccccttctac ttgcccagta aaaaatcgat cagcgcgtcg cggacttgct
      661 gctcctttgc gtccgccggg cagttcagct ccagcaggca tccggggctt gcaatctccc
      721 agaggcgatg cgcgtccgag gaaacgatct tccgcttccc cctgaggcgc gggtactgcg
      781 cttcataggc ggatgcgttc gttagctcgc ggtactcgac cgccgcaaac gcgggactct
      841 ccggcaggtc gcccagcacg gcgataacgc cgttgccttc gcggtccaca tgcgcgggaa
      901 ttgcggctcc gccgtggatg ctcgccagct gcgccgcttc ttccagcgaa agcagggtgg
      961 cgttgatcaa caagtaaggt tcccgaccgg tgggttcgtc gtcaaggccg atcaggagct
     1021 gattgccaaa gactttctcg cggttcggaa tccgaatccg catgttctcc acttcggcgg
     1081 aaaacgcctt tgcgtcttcc agcgtacgga agaggcagag aaggtgcacg tcctcgctgg
     1141 tggtcagttc catcccggcg accgggacga ttccgtaccc ccggcaagcc tgaaaaaacg
     1201 attcacagtt gccgcaggcg ttgtggtcgg tcagcgccgc aacggcgagg ccgttgagct
     1261 ttgccatgcc ggcaatcgag tacggcgtca tatcgtcgtc cgcgcagggc gacaggcagg
     1321 agtgaatatg cagatcgcag cgaaagccgc tcatatcagt ccggcaatcc gcccgcagag
     1381 cgcaaacgcg gattccccgc tgcccagcag gttgatcccc ctggcaagcg caatctctgt
     1441 tactcccgcg tccggcgcaa cgccttcggc aagaacgatg caggcaacat ccgccagcgt
     1501 agcgaccgcg acgatgttgg gattgctcat aatcgtcacc caggcgtcgc cttcgtttgc
     1561 gcgccccatc acccagctga gcaaatcccc cgcgtagccg ccggtgatct tccgctcgcc
     1621 gtccgaaacg acaaacggct caa
//
LOCUS       DAOMGY010000436         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_87830_length_1641_cov_3.353090, whole genome shotgun sequence.
ACCESSION   DAOMGY010000436 DAOMGY010000000
VERSION     DAOMGY010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87830_length_1641_cov_3.353090"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLR57_06940"
     CDS             <1..782
                     /locus_tag="PLR57_06940"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HQQ41229.1"
                     /translation="SAIAAGARLIKTSAGDDGQLSLEAFAGILRARGMDLGLDAGLAM
                     TELSRTAGQVRWMVSARSNSATPYDNSVQRKIDASVCLSTSSTISEIASLVKKLGYDL
                     SEEDTAKVYDAFLRIADKKDVGEKELDAIVASAALQVPPTYTLESYVINSGNVITATA
                     HIKLLRQGKTQQGFGLGDGPIDAAFLAIEQIIGHHYELDDFQIQSVTEGREAMGSALV
                     RLRANGKLYSGNGISTDIIGASIHAYLSALNKIVYEEGGRT"
     gene            835..>1641
                     /locus_tag="PLR57_06945"
     CDS             835..>1641
                     /locus_tag="PLR57_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQQ41230.1"
                     /translation="MKLSFSTRAWNAYSWDEFTATAKAVGYSGIELHGLIGTPWTEDG
                     APFSRANTGKTVRSLFDEGIRLTCLDSVCDLSSGNAEEEEELFACIRLAHDFSIPYVR
                     VRAFDASETGLSAAYERVLRALPYAEERGITLLIETVGAFSDTALLQKFLMRFSSDTL
                     AALWDLHHSYFEHGESPETSIKNLGAYVKHVHVKDSALESGVRAYCLPGEGELPMAEL
                     MTALSSINYDGFISLEWDPQWMPELDDIESVLAHFANYMQRFGTPARGKKR"
BASE COUNT          350 a          485 c          486 g          320 t
ORIGIN      
        1 tcagcgcaat cgccgccgga gcgaggctga ttaaaacgtc cgcgggcgac gacggccagc
       61 tctcgctgga ggcgttcgcg gggattctcc gcgcgcgcgg catggatctc ggcctcgacg
      121 cgggtttggc tatgacggag ctcagccgaa ccgccggaca ggtgcgctgg atggtcagcg
      181 cgcgcagcaa ctccgctacg ccttacgaca acagcgtaca gcgcaagatc gacgcaagcg
      241 tttgcctctc cacttcgagc actatttcag agattgcgtc tctggttaaa aagctcggct
      301 acgacctttc ggaagaggat acggcaaagg tttacgatgc gtttttgcgc attgcggata
      361 aaaaagacgt cggcgaaaaa gaactcgacg caatcgtggc gagcgcggcg cttcaggtgc
      421 cgccgaccta cacgctggaa agctatgtga tcaacagcgg caacgtcatc accgccacgg
      481 cgcatatcaa gctcctgcgg cagggcaaaa cccagcaggg cttcggcctt ggcgacggcc
      541 cgatcgacgc ggcgttcttg gccattgagc agatcatagg ccaccactac gagctggacg
      601 acttccagat tcagtcggtc acggaagggc gcgaggcaat gggttcggcg ctggttcgcc
      661 tccgcgcaaa cggcaagctc tattccggca acggtatctc cacggacatc atcggcgcga
      721 gcatccacgc ctacctcagc gcgctcaaca agatcgtgta cgaggagggc gggcgaacat
      781 gaaccgtttg atttccgcca aaatcaggct gcctcagcgg gaaggcggta aaatatgaaa
      841 ctgtcgtttt caacccgcgc atggaacgct tactcctggg acgaatttac agccaccgca
      901 aaagcggtcg gttattcggg catcgagctc cacgggctga tcggtacgcc ctggacggag
      961 gacggcgcgc cgtttagccg tgccaacacg ggtaaaaccg tccgttcgct gtttgacgag
     1021 ggaattcgcc taacctgtct ggactccgtc tgcgacctct cttccggcaa tgccgaagaa
     1081 gaggaagaac tgtttgcctg catccggctg gcgcacgatt tttcaattcc gtacgttcgc
     1141 gtccgcgcgt tcgatgcaag cgaaaccggg ctttccgccg cttacgaacg cgttttacgc
     1201 gcgctgccat acgccgaaga gcgcggaatt acgcttttaa tcgaaaccgt cggcgcgttt
     1261 tcggataccg cgcttctgca gaaattcctg atgcgttttt cgagcgatac gctcgccgcg
     1321 ctttgggatc tgcatcactc gtacttcgag cacggcgaat cgcccgaaac cagcatcaaa
     1381 aacctcggcg cgtacgttaa gcacgtacac gttaaggatt ccgcgctgga aagcggcgtt
     1441 cgcgcctatt gcctgccggg cgaaggcgaa ctgccgatgg cggagctcat gacggcgctt
     1501 tcctccatta actacgacgg gtttatctcg ctggagtggg atccgcagtg gatgccggaa
     1561 ctggacgata ttgagagcgt gctggcgcat tttgccaact atatgcagcg cttcggcacg
     1621 cccgcgcgcg gaaaaaaacg g
//
LOCUS       DAOMGY010000437         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_87886_length_1641_cov_1.998108, whole genome shotgun sequence.
ACCESSION   DAOMGY010000437 DAOMGY010000000
VERSION     DAOMGY010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87886_length_1641_cov_1.998108"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLR57_06950"
     CDS             complement(<1..533)
                     /locus_tag="PLR57_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41231.1"
                     /translation="MDNVQLRTYVTIDNMQPQYAAATGTVVKGDTCLAGMAQLYIEMS
                     PGSEIYRLLDVALKSSSAKPGFLVVEREYGELELHSVNIDDVTEAGRAILAQCNLTLA
                     DRLKPRVVSRQIIQNVDPYQAQLINRGRYGSLLLPGENLLILEVEPAAYISIAVNEAA
                     KSSRIKVVSFDPVGRYGR"
     gene            complement(603..881)
                     /locus_tag="PLR57_06955"
     CDS             complement(603..881)
                     /locus_tag="PLR57_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HQQ41232.1"
                     /translation="MKAKGFIETKGLVAAYEAADAMIKAANVELVDKQQVGGGLVTVI
                     IEGDVGAVKAAVDAGANAAKKVGEVIAAHVIARPQAEVETLFSGERQK"
     gene            complement(996..1289)
                     /locus_tag="PLR57_06960"
     CDS             complement(996..1289)
                     /locus_tag="PLR57_06960"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HQQ41233.1"
                     /translation="MYIGKVTGRVVSTIKDPRLTGISLVLVERLPARAGQESEVFVAA
                     DTIGCGEGNTVLLAKGSAVRAVASLQNTPVDLAVIGIIDNAELPQETGKKKAK"
     gene            complement(1303..>1641)
                     /locus_tag="PLR57_06965"
     CDS             complement(1303..>1641)
                     /locus_tag="PLR57_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41234.1"
                     /translation="SFAAGAGIKLANDIGQQGISERIDYLSEIALLKADQLGLKCVSP
                     HDVRKKGGTTAIEIEMDSHHMEAILRDRDIIASSRGNVIRIAPHFYTLPHEVEFAFEQ
                     IRDIIDHGGH"
BASE COUNT          281 a          471 c          483 g          406 t
ORIGIN      
        1 cgcccgtagc gtccaacggg atcgaaggac acaaccttga tgcgcgagct ctttgccgct
       61 tcgtttacgg caatggagat atacgccgcc ggttcaacct cgagaatcag caggttttcg
      121 cccggcagca ggagcgagcc gtagcgtccc ctgttgataa gctgcgcctg ataggggtct
      181 acgttctgta tgatctggcg cgaaacgacg cggggtttga ggcggtccgc aagcgttaga
      241 ttacactgcg cgaggatggc tcttcccgcc tccgttacgt cgtctatgtt tacggagtgc
      301 agttccagct caccgtattc gcgctcgacc acgaggaacc ccggctttgc ggaggaggac
      361 ttcagcgcaa cgtcgagcag gcggtagatt tcgctgcccg gactcatttc aatgtagagc
      421 tgcgccattc cggctaggca ggtgtcgcct ttgacgacag tgccggtcgc agctgcgtac
      481 tgcggctgca tattgtcgat tgtcacgtag gtacgcagtt gaacgttatc cattgaactc
      541 tcctttatcg ccgcgcggcc gcggtgctct cagaatgagg gtagtgccgt agggaaagca
      601 agttatttct ggcgttcgcc ggagaacagc gtttccactt ccgcctgggg acgggcaatg
      661 acgtgtgccg cgatcacttc gccgaccttc ttggccgcgt ttgcgcccgc gtcgaccgcc
      721 gctttgaccg cgccgacgtc gccttcgatg atgacggtta cgagaccgcc gccgacctgc
      781 tgtttgtcga cgagttcaac gttcgcggcc ttgatcattg catccgccgc ttcatacgcc
      841 gccacgagac ccttggtttc gataaaaccc tttgctttca tttttgagtg ctccttttca
      901 tatttttatt gtttgattcg cttactggct tctgccgcct tcgggcggac tgccgtttgt
      961 gttcggtgat atcgttttct gattaaaagg aaacgttact tcgctttttt ctttccggtt
     1021 tcctgcggca gttcggcgtt gtcgataatg ccgatgaccg ctaggtccac cggggtattc
     1081 tgcaggctgg cgacggctct gacagcgctg cctttggcaa gaagcaccgt gttcccttcg
     1141 ccgcagccaa tggtgtccgc cgcaacgaaa acttcgctct cctggcctgc gcgcgcgggc
     1201 agccgctcga cgagcaccag agaaattccg gtgaggcggg ggtccttgat ggtggagacg
     1261 actctgccgg tcactttgcc gatatacata tagtcacctt cctcaatggc cgccgtggtc
     1321 gatgatatcg cgaatctgct cgaacgcgaa ttccacctcg tgcggcagcg tgtaaaaatg
     1381 cggggcgatg cggattacgt ttccgcgcga ggaggcgatg atatcgcggt cgcgcagaat
     1441 cgcttccatg tggtgggagt ccatttcaat ttcaatggcg gtggtgccgc cctttttgcg
     1501 cacgtcgtgc gggctgacgc acttgaggcc gagctgatcg gccttgagca gcgcgatctc
     1561 gctgagatag tcgatccgct ccgaaatgcc ctgctggccg atgtcgtttg ccagctttat
     1621 gcctgcgcct gcggcaaagg a
//
LOCUS       DAOMGY010000438         1640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_87918_length_1640_cov_3.603785, whole genome shotgun sequence.
ACCESSION   DAOMGY010000438 DAOMGY010000000
VERSION     DAOMGY010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87918_length_1640_cov_3.603785"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLR57_06970"
     CDS             <1..337
                     /locus_tag="PLR57_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HQQ41235.1"
                     /translation="VEGKISDERFAKMSASYEEEQKGLESRVTELKKLIAAEKESSVN
                     VDRFLALVRKYTDVKELTAEIIREFVEKIYVYQSERIDGHKAQRIKIVWNCIGEFTPP
                     VITDKEKSA"
     gene            complement(434..>1640)
                     /locus_tag="PLR57_06975"
     CDS             complement(434..>1640)
                     /locus_tag="PLR57_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41236.1"
                     /translation="AMLLIPAGVIYAIKAAPSFWYYPTFLLVTLLIPAVPTIIGAMLG
                     TLLTIGTARMKKRNIMNILGQLLLVFVIMFFSFRMNTSIADPGKLAAGAGALQSSVSG
                     VYPPAQWFQSALTEGSLTSLLWLLLLTAASIALLLLFVGRYFVAVNSRIKSQPQGEKF
                     VMRSQARSSSTTALFKNEWRRYLSSSLYVVNTAFGYLMLLGAGVFCVIKPDMIASVLN
                     MPETSFLHLLVPFILGWILSMSATTSSAISMEGKRLWIVKSMPVPARDWLTSKLMVSL
                     VLAVPSVLVACTLISIGLRSDAAGWFWNYVIPLLYAVAFAVFGLWINIRMPRMDWQNE
                     AEVVKQSGSVMIAIFTGMGVAAIPAIVIGFTQSIWVTPLTALVLLALTLAMWRSLVKS
                     GDKRLYKLN"
BASE COUNT          423 a          433 c          479 g          305 t
ORIGIN      
        1 tgtcgaaggc aagatttccg atgagcgttt tgcaaagatg agcgccagct atgaggaaga
       61 acagaaaggt ctggaaagcc gggtgaccga gctgaaaaag ttgattgctg ccgaaaagga
      121 aagttccgtg aatgttgacc gcttcttggc tcttgtacgg aagtacacgg acgtcaaaga
      181 actgaccgca gagatcatcc gggagttcgt tgagaaaatc tatgtgtatc agtctgaacg
      241 cattgacggg cacaaggcgc agcgtatcaa aatcgtatgg aactgcatag gcgaattcac
      301 accgccagtt attactgaca aagaaaaatc ggcatagccg gtaaatacat accaaactat
      361 gccgatattt ttttccggga ttgaaaatcc ctaatttgca ccacctaaag gcagggcgtt
      421 tgttgttttg gagttagttg agcttgtaaa ggcgtttgtc gccggatttg acgagcgagc
      481 gccacatggc aagcgtcagc gccagaagca ccagcgcggt cagcggagtg acccaaatac
      541 tctgcgtaaa cccgataacg atggcgggaa ttgctgcaac gcccatgccg gtaaagatcg
      601 caatcatcac cgagccgctc tgcttgacga cttccgcttc gttctgccag tccatgcgcg
      661 gcatacggat gttgatccaa aggccgaaga ccgcaaacgc gacggcgtac aagagcggga
      721 taacgtagtt ccagaaccag cccgccgcgt cggagcgcaa gccgatggag atcagcgtgc
      781 aggcaacgag cacgctcggt acggccagca caagcgacac catgagcttg gaggtcagcc
      841 agtcccgcgc gggaaccggc atgctcttga cgatccagag ccgcttgcct tccatggaga
      901 tagcgctgga agtcgtcgcg ctcatcgaaa ggatccagcc gaggataaac ggtaccagca
      961 ggtgcaaaaa ggatgtttcc ggcatgttca gcacgcttgc aatcatatcc ggcttgatca
     1021 cgcagaacac gcccgcgccc agcagcatga ggtagccgaa cgcagtgttg accacataca
     1081 ggctggagct aaggtatctg cgccactcat ttttaaacag tgccgtcgta ctgctggaac
     1141 gggcctgcga acgcatgacg aatttttcgc cctgcggctg gctcttgatg cgggaattga
     1201 ccgcaacaaa gtacctgccc acaaacagca gcagcagcgc aatggaagca gccgtcagca
     1261 gcagcagcca aagcaggctc gtcagcgagc cttccgtaag cgcgctctgg aaccactgcg
     1321 ccggcggata gacgccggaa acggaggact gcagcgcgcc tgcgcccgcg gcgagtttgc
     1381 ccgggtcggc aatgctggtg ttcatgcgga acgagaagaa cataatcacg aatacgagca
     1441 gcaactgccc gagaatattc atgatgttgc gcttcttcat ccgcgcggtg ccgatggtta
     1501 acagcgttcc cagcatggcg ccgatgatgg tcggcacggc gggaatcagc agcgtgacaa
     1561 gcaggaacgt cgggtaatac cagaacgagg gcgcggcttt aattgcgtag attacgccag
     1621 cgggaatcag gagcatggcg
//
LOCUS       DAOMGY010000439         1637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_88191_length_1637_cov_2.940582, whole genome shotgun sequence.
ACCESSION   DAOMGY010000439 DAOMGY010000000
VERSION     DAOMGY010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88191_length_1637_cov_2.940582"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1108
                     /locus_tag="PLR57_06980"
     CDS             38..1108
                     /locus_tag="PLR57_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005211161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQQ41237.1"
                     /translation="MKKECRLGKQHPPNGRLILPVLFSVIVFIIFLITSAIIMLVSVI
                     LLQSGAINLAKLSRQEPIVPFALMLLLSILIGTVVSFIISRIPLRPIRRVIDAINRLA
                     AGDFSVRLSSTVTPTFRELVESFNRMAEELGGIELLRTDFINNFSHEFKTPIVSIKGF
                     AEELKHDDLSNEQRDEYLDIIIAESSRLASLATNVLNLSKIEQQVILTDQKAFNLTEQ
                     VRRCILLFENKWEKKHISLNVEMEELSYTGSEELLNQIWLNLIDNAVKFTPQGGVIGI
                     RLSKSGDALQFVIADDGCGISEEALSRVFDKFYQADPARAGSGNGIGLTVAKRIAELH
                     GGTISCRSQEGVGTEFTVTLPL"
     gene            1253..>1637
                     /locus_tag="PLR57_06985"
     CDS             1253..>1637
                     /locus_tag="PLR57_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQQ41238.1"
                     /translation="MDTKKKKSRVWIWIVGVIVVLIVAALVLIRNAISNASQTVYASY
                     TLASGSLETTITGSGKLESADSQTLQLPAGVTVSDVLVEAGDAVKAGDTLALLDPASL
                     ANRAAEVSSELSSLDRQIASRSTTGS"
BASE COUNT          365 a          486 c          438 g          348 t
ORIGIN      
        1 ccgtgcgcgg gcttggctac aaggtggtga agaaagtatg aaaaaagaat gccggctggg
       61 caaacagcat ccgccgaacg gaaggctcat cctgccggtg ctgttctcgg tgatcgtatt
      121 catcatcttt ttaataacgt ccgccatcat catgctcgtc agcgtcatcc tgctgcaatc
      181 cggcgcgatc aaccttgcca agctctcgcg gcaggagccg atcgttccgt ttgcgctgat
      241 gctgctcttg agcattctca tcggcaccgt ggtttcgttt atcatcagcc gcatcccgct
      301 tcgccccatc cggcgcgtga tcgacgcaat caaccggctt gccgcgggag acttctccgt
      361 acgcctctcc tccaccgtca cgccgacgtt tcgtgagctt gtggaaagct tcaaccgcat
      421 ggccgaagaa ctgggcggca tcgagctgct gcgaacggac tttatcaata acttttccca
      481 cgagttcaaa accccgatcg tctcgatcaa gggctttgcc gaagaactca aacacgacga
      541 tttatccaac gaacagcggg atgaatatct cgacatcatc attgccgagt cgagccgcct
      601 tgcttcgctc gccacgaacg tgctcaacct ttccaaaatc gaacagcagg tgattctgac
      661 cgatcagaag gcgttcaacc tcaccgagca ggtacgccgc tgcattctgc tctttgaaaa
      721 caagtgggaa aagaagcata tcagcctgaa tgtcgaaatg gaggagcttt cctacaccgg
      781 cagcgaggag ctgctcaatc aaatctggct caacctgatc gacaacgccg tcaaatttac
      841 gccccagggc ggcgtaatcg gcatccggct gagtaagtcg ggcgatgcgc tccaatttgt
      901 catcgcggac gacggctgcg gcatctccga agaagcgctc tcgcgcgtgt tcgacaaatt
      961 ctatcaggcg gatcccgcgc gtgccggcag cggcaacggc atcggcctga ccgttgcaaa
     1021 gcgcattgcc gaactgcacg gcggaacgat ctcctgtcgc agtcaggaag gcgtaggcac
     1081 cgagtttacg gtaacgctgc cgctgtaacg gcgcgaatct acggcgataa cccggccatt
     1141 tgcggcaatt ttgtgaaacg cgtacaaaaa aacacgtttt gcgttttcag tttcttttca
     1201 gttcatgttc ggcggatatt gtgagcgccg tagcaaataa ggaggtatcc caatggatac
     1261 aaagaagaaa aagtcaagag tatggatttg gatcgtcggc gtgatcgttg tgctaatcgt
     1321 agcggcgctt gtgctgattc gaaatgcaat cagcaacgct tcccaaacgg tctacgcttc
     1381 ctatacgctc gcttccggca gtctggaaac caccatcacc ggcagcggca agcttgaatc
     1441 ggcggacagc caaaccctgc agcttcccgc gggtgtgacc gtgagcgacg tgctcgtgga
     1501 agccggcgac gcggtcaaag cgggagatac gctcgcgctg ctcgaccccg cttcccttgc
     1561 caaccgcgcg gcggaagtct ccagcgagct ttcctcgctg gatcgccaga tcgcttcccg
     1621 ctccaccacc ggctcgg
//
LOCUS       DAOMGY010000440         1636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_88296_length_1636_cov_2.339026, whole genome shotgun sequence.
ACCESSION   DAOMGY010000440 DAOMGY010000000
VERSION     DAOMGY010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88296_length_1636_cov_2.339026"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLR57_06990"
     CDS             <1..703
                     /locus_tag="PLR57_06990"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HQQ41239.1"
                     /translation="DLEGTDFADLLQRVSRVKGLERIRFMTSHPKDLSERVIAAMASL
                     PNVCHHVHLPLQSGSNEILRRMNRKYTREQYLTIVKKLREAMDDVEITTDIIVGFPGE
                     TEQDFAETLDLIEQVGFAAAFTFKYSPRKGTRAADYPDQVPEPVKRERLRVLNDLQER
                     KSRENNEKYLGASGVVHVEDCDTRNEPVCYGKFSNFKMVYFPGGPELIGQYVRVRVKQ
                     VRKNSLSGEMEENNL"
     gene            700..1089
                     /locus_tag="PLR57_06995"
     CDS             700..1089
                     /locus_tag="PLR57_06995"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HQQ41240.1"
                     /translation="MIELARELGLALANSAEFIRMKNAQNAVEQNEGINAALTELREK
                     RERLVNILAEEETDNLEALRLTNDIDRLEGQLQDNPLFLELLEAQAAFSTVLRTINDE
                     INACIGGETSESASCEGDCGSCGGCKH"
     gene            1103..>1636
                     /locus_tag="PLR57_07000"
     CDS             1103..>1636
                     /locus_tag="PLR57_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQQ41241.1"
                     /translation="MPSSMTPMMRQYIELKEKYPDCLLFFRLGDFYEMFFEDAKTASK
                     ELDLVLTGRDCGLSERAPMCGVPYHAVDNYIAKLIEKGFKVAVCEQMEDPALAKGLVE
                     RDVIRVITPGTVIEERMLEENQNSYIASVSLGDQAIGLSYADVSTGEFYLLQLEGEQA
                     AVALLDEMERIRPREIIA"
BASE COUNT          406 a          426 c          480 g          324 t
ORIGIN      
        1 cgatctggaa ggcacggatt ttgcagatct gcttcaacgg gtttcccgtg ttaaaggact
       61 cgagcggatt cgattcatga cctcgcatcc aaaggatttg agtgagcgcg tgatcgccgc
      121 catggcgagc ctgcccaacg tttgccatca tgtgcatttg ccgctgcaga gcgggtcgaa
      181 tgagattctg cgccgtatga accgcaagta tacccgcgag caatatctca caatcgtaaa
      241 gaaactccgc gaggcaatgg acgatgtgga gatcacgacc gatatcatcg tcggttttcc
      301 cggcgaaacg gaacaggact tcgccgaaac gctcgattta attgagcaag tggggtttgc
      361 tgccgcgttc accttcaagt attcgccgag gaaggggacg cgcgcggcgg actatcccga
      421 tcaggtgccg gaaccggtta agcgcgaacg cctgcgcgtg ctcaacgacc tgcaggagcg
      481 aaaatcccgc gagaacaacg aaaaatatct gggcgcttcc ggcgtcgtgc atgtggaaga
      541 ttgcgatacc agaaacgaac cggtttgcta cggaaagttt tcaaatttca aaatggtcta
      601 cttcccgggt ggtccggaac tgatcggaca gtatgtgcgc gtccgcgtta aacaagtaag
      661 aaagaactct ctttcgggag agatggagga aaacaacctg tgatcgaatt agcaagagaa
      721 ttgggactcg cgctggccaa ctccgccgag tttatccgca tgaagaacgc acaaaacgcc
      781 gtcgaacaga acgaaggcat caacgccgca ctcaccgagt tacgggaaaa gcgggagcgc
      841 cttgtaaaca ttcttgccga agaagagacg gacaatctgg aagcgctccg cctgaccaac
      901 gatatcgacc gtctggaagg gcagcttcag gacaatccgc tgtttttaga actcctggag
      961 gcgcaggcgg cgttttcgac cgtgctgcgc acgattaacg acgaaatcaa cgcctgcatc
     1021 ggcggcgaaa caagcgaaag cgcaagctgc gagggcgact gcggcagctg cggcggctgc
     1081 aagcactaag cggagggaac gcatgccatc ttcgatgacg ccgatgatgc ggcagtatat
     1141 cgagctgaaa gagaaatacc ccgattgcct gctgtttttc cggctgggcg atttttacga
     1201 aatgttcttt gaggacgcaa aaaccgcctc aaaggagcta gatctcgttt taacggggcg
     1261 ggactgcggg ctcagcgaac gcgcgccgat gtgcggcgtt ccttaccatg cggtcgataa
     1321 ctatatcgcc aagctcattg aaaagggctt caaggtagcc gtatgcgagc agatggaaga
     1381 tcccgctctc gccaaaggat tggtcgagcg ggacgtcatc cgtgtgatta cgcccggtac
     1441 ggtcattgag gagcgcatgc tggaagagaa ccagaacagc tatatcgcct ccgtttctct
     1501 gggcgatcag gcgatcgggc tttcatatgc ggacgtttcc acgggagaat tctaccttct
     1561 ccaactggaa ggcgaacagg cagccgtcgc gctgctggat gagatggagc gcatccgccc
     1621 gcgcgagatc attgcc
//
LOCUS       DAOMGY010000441         1633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_88513_length_1633_cov_2.350444, whole genome shotgun sequence.
ACCESSION   DAOMGY010000441 DAOMGY010000000
VERSION     DAOMGY010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88513_length_1633_cov_2.350444"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..634)
                     /locus_tag="PLR57_07005"
     CDS             complement(26..634)
                     /locus_tag="PLR57_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41242.1"
                     /translation="MTKSVYSLVLSDEIVREIDRLAYEAGESRSAMINQVLADFVRYT
                     TPEKRMREVFGAIEHMLTGGVFEPQLQPSDSMFSLKSALDYKYNPSVRYSVELYKNAL
                     PLLGELRVSVRSQNSALVLAMLQFFKLWTRIETAYTGRVECAMEDGRYARKLRFPEAD
                     ADNGAVGEGIAAYISLLDRALKSYFESINEPALAVRNVERRI"
     gene            833..>1633
                     /locus_tag="PLR57_07010"
     CDS             833..>1633
                     /locus_tag="PLR57_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQQ41243.1"
                     /translation="MKYNFDELIGRSNTHSLKYEACRKINPFLPEGSIPLWVADMDFA
                     CAPQILAAMQRRLENRILGYSVLGEDTQAAVSRWMDRRFGWTVAPEEIVFASGVVTAL
                     YAAVEHLTKPGDAVLTMTPAYRPFDTAMKNYSRTPLYNRMIEGADGRYSIDFADFEEK
                     AKRPDCTMFFLCNPQNPTGRVFAKEELLRIGEICFANSVFVVSDEIHADLTRTGQTHI
                     PFAKLFPDEKRIITCTSPSKTFNIAGNNHAHLMIPNEKIRREWSSRSYC"
BASE COUNT          350 a          486 c          428 g          369 t
ORIGIN      
        1 caaacggatt cgtcgtgatc gaggatcaga tgcggcgctc cacgttccgc acggccagcg
       61 cgggttcgtt gatcgattca aaatagctct tgagcgctcg gtcgagcagg ctgatgtacg
      121 ccgcgatccc ctcgccgacg gctccgttat ccgcgtcggc ctccgggaag cggagctttc
      181 gcgcgtaccg cccgtcctcc atggcgcact cgaccctgcc ggtatacgcc gtttctatgc
      241 gcgtccaaag cttgaaaaac tgcagcatgg ccagcaccaa agcgctgttc tggctccgca
      301 cgcttacccg gagctcgccc agcagcggaa gagcgttttt atagagctcc acactgtagc
      361 gcacgctggg gttgtattta taatccagcg cgctcttgag cgaaaacatg gagtccgaag
      421 gctgcaactg cggctcgaat acgccgccgg tgagcatatg ctcaatggct ccgaatacct
      481 ctcgcatgcg tttttccggc gtggtgtagc gcacaaaatc ggccagtacc tgattgatca
      541 tggctgaacg gctttcgccc gcttcatagg ccagccggtc gatttcgcgc acgatctcgt
      601 ccgacagcac gagcgaatac acgctttttg tcatttcgga tcactcctta cctttacgaa
      661 ccgaaacggc aggcagttcg cccgtttttg ttcggttcgt atgcctttat tatatccgcg
      721 cgtataaact ttgcaagcgt tgttaataat tttgactgcg attttgcaat tctttcacga
      781 tttgactata ctgatctcgc aatgcttttt tatctgggga gaacacagga gaatgaagta
      841 caattttgac gaactgatcg gccgctcgaa cacgcacagc ttaaagtacg aagcctgccg
      901 gaagatcaat ccttttctgc cggaaggttc gattccgctt tgggtggcgg atatggactt
      961 tgcctgcgcg ccgcagattc tcgctgccat gcagcgcagg cttgaaaacc gcattctcgg
     1021 ctacagcgtg cttggcgagg atacgcaggc cgccgtctcc cgctggatgg atcgccggtt
     1081 cggctggact gtcgcgccgg aggagatcgt ttttgccagc ggagttgtta ccgctctgta
     1141 tgccgccgtc gagcacctga ccaaacccgg cgacgcggta ctgaccatga cgcccgccta
     1201 ccgtcccttt gatacggcaa tgaaaaacta cagccgcacg ccgctctaca accgcatgat
     1261 cgagggtgcg gacggtcgct attccatcga ctttgccgac tttgaagaaa aggcaaaacg
     1321 tcccgactgc acgatgtttt ttctctgcaa cccgcagaat cccaccggac gcgtgtttgc
     1381 gaaagaggag cttctgcgca tcggcgaaat atgcttcgcc aacagcgtgt tcgtcgtaag
     1441 cgacgaaatt cacgccgacc tcacgagaac gggacagacg catatcccgt ttgccaaact
     1501 gttccccgat gaaaaacgca tcatcacctg cacaagcccc agcaagacct tcaacattgc
     1561 gggcaacaac cacgcgcatc tgatgattcc gaacgagaag attcgccgcg agtggtcgag
     1621 ccgctcctac tgc
//
LOCUS       DAOMGY010000442         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_89024_length_1627_cov_3.138041, whole genome shotgun sequence.
ACCESSION   DAOMGY010000442 DAOMGY010000000
VERSION     DAOMGY010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89024_length_1627_cov_3.138041"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PLR57_07015"
     CDS             complement(<1..743)
                     /locus_tag="PLR57_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HQQ41244.1"
                     /translation="MEGITFHRLGQSFEESGGGGPATVLLSKQGNLEIMSHKIGSEFV
                     VWLMPASDPAAMEFFFVYEGGVELALDDGVVALGPGESFTVSGLAREIPVKMLGDTRI
                     LYVTNCPAFESVAQFEESLLALLVRINEKDHYTYKHSGSVLKYALCLYDAMESKMETV
                     SRNDMAVAALFHDVGKCFIPVEILQKPEALEPAETRYILRHPLDSGRMLRAEFGEAVA
                     ETAMNHHERLDGSGYPRGLTSEDIGLPAR"
     gene            complement(776..1114)
                     /locus_tag="PLR57_07020"
     CDS             complement(776..1114)
                     /locus_tag="PLR57_07020"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQQ41245.1"
                     /translation="MLRVCEDLSCIFPQHADVMAKVCEKISKLSGAERIVLFGSYAKG
                     TAEAESDVDLAVFFRAEEECLVAQYRALTRICANPELDIQVQPFHTYELLQPCGIIEE
                     VVTYGIELKL"
     gene            complement(1160..1537)
                     /locus_tag="PLR57_07025"
     CDS             complement(1160..1537)
                     /locus_tag="PLR57_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41246.1"
                     /translation="MSKKVLSILLVVMLVILMLPGMASAKGNSPELKAVALNGHHETA
                     DQLKKLEKMVDKANDEIERAVAKAQRTPEDDVAELLIIIDQIVAEVSAYAESIGYAIT
                     CDYVTYEIDGQIVLIDPLRIIPL"
BASE COUNT          384 a          464 c          386 g          393 t
ORIGIN      
        1 atccttgcgg gaaggccgat atcttcggac gtcagccctc tgggatagcc gcttccgtcg
       61 aggcgttcat gatgattcat cgcggtttcg gcaaccgcct cgccaaactc cgcccggagc
      121 attctgccgc tgtcgagcgg gtggcggagg atataccgcg tctccgctgg ttccagcgct
      181 tccggctttt gcaggatttc gacgggaata aagcatttgc caacgtcgtg aaacagcgcc
      241 gcgaccgcca tatcgtttct ggataccgtt tccattttgg attccatcgc gtcgtagaga
      301 caaagcgcat atttcaaaac ggaaccgctg tgcttatagg tataatggtc tttttcgttg
      361 attctaacga gcagcgcaag caggctctct tcaaactgcg caacggattc gaacgccggg
      421 cagttcgtta catacaggat tctcgtatcc cccagcatct tgaccggaat ttcgcgggca
      481 agcccggaaa ctgtaaacga ttctcccggt ccaagcgcaa caacgccgtc gtccagcgcg
      541 agttccacgc cgccttcgta aacaaagaaa aactccatgg cagcgggatc gctcgccggc
      601 atcagccaga cgacgaactc actgccaatt ttgtgcgaca taatctcaag gttcccctgt
      661 ttggacagga gaaccgtcgc ggggccgcct ccgccgcttt cttcaaacga ctgaccaagc
      721 ctgtgaaatg tgattccttc cattgcaaca cctactcacg agcgcttgtt tggtatcaaa
      781 gtttcagctc aatcccgtaa gtaactacct cttctatgat accacaaggc tgaagcaatt
      841 cataggtgtg gaacggttgg acttgtatgt ccagctccgg attggcgcag atacgcgtca
      901 gcgcccggta ctgggcaaca agacattctt cttcggctcg aaagaacacc gcaagatcaa
      961 catcgctctc ggcttcggcg gtaccttttg cataagaacc aaaaaggacg atccgctcag
     1021 caccggacag cttggaaatt ttctcgcata ctttcgccat cacatcagcg tgctgtggaa
     1081 agatacacga taaatcctcg caaacccgaa gcatgcagaa cgttcctttc ggttaatagc
     1141 gaatgggaaa tcgtttctat taaagaggaa tgatacggag cggatcgatc aaaacgatct
     1201 gtccgtcaat ttcatacgtt acataatcgc aggtgatggc atagccaatc gactcggcgt
     1261 acgcgctgac ttccgcaacg atctgatcga tgataatcaa cagctccgca acgtcatctt
     1321 cgggcgtacg ctgcgctttc gcaaccgcgc gctcgatttc gtcgttggcc ttatcgacca
     1381 tcttctccag ctttttgagc tgatcggcag tctcatgatg cccgttcagc gcgaccgctt
     1441 tcaattcggg gctgttgccc ttcgcgcttg ccatacccgg caacatgaga atcaccagca
     1501 taacgaccag tagtatgcta agtacctttt tgctcatgat ccgattcctc ccgaaatttt
     1561 caaattagga tagaattcgg agtattcccg cgctttcggc aatgaaaaaa gcagcctgcc
     1621 tctttcc
//
LOCUS       DAOMGY010000443         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_89057_length_1627_cov_1.954835, whole genome shotgun sequence.
ACCESSION   DAOMGY010000443 DAOMGY010000000
VERSION     DAOMGY010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89057_length_1627_cov_1.954835"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLR57_07030"
     CDS             <1..328
                     /locus_tag="PLR57_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQQ41247.1"
                     /translation="PDPASLGPRTKGKTNIGCVITGVKDGKEKTIFIYNVCDHEECYR
                     ELGSQAISYTTGVPAMIGAALVLNGTWKKPGVFTTDEFDPDPYMDMLNKWGLPWVVDE
                     HPVTVE"
     gene            330..1456
                     /gene="nspC"
                     /locus_tag="PLR57_07035"
                     /pseudo
     CDS             330..1456
                     /gene="nspC"
                     /locus_tag="PLR57_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004432020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
BASE COUNT          334 a          468 c          519 g          306 t
ORIGIN      
        1 gcccgacccc gcgtcgctcg gtccgcgcac gaagggcaaa acgaacatcg gctgtgtcat
       61 caccggcgta aaggatggca aggagaaaac gatcttcatc tacaacgtct gcgaccacga
      121 ggaatgctac cgcgaactgg gcagtcaggc catcagctac accaccggcg tgcccgcaat
      181 gatcggcgct gcgctggtcc tgaacggaac ttggaaaaag cccggcgtgt ttacgaccga
      241 cgagttcgac cccgatccgt atatggacat gctcaacaaa tgggggctgc cttgggtggt
      301 ggacgagcac ccggttacgg tggaataacg tgaagcggca ggaactgcct acgccctgct
      361 ttgtgatgag cgaagcggcg ctgaagcgca atctggagat attaggcggc gtgcaggaac
      421 gcacgggcgc taagattttg cttgcgcaaa aggcgttttc tatgttccgc gtgtaccctc
      481 tgatcgcgcg gacgcttagc ggcgcgtcgg cgagcgggtt gtatgaagcg cggcttgcgc
      541 acgaggagat gggcggtgag aaccatgtgt tttcgcccgc gtatacggtc gatgagatgc
      601 gcgagttggt gaacatctgc gaccatatcg tgttcactcg tttgcacagc tggaagcgta
      661 ccgccccgtt tggcagagcg cgagcgtcag cgtcggcatc cgcataaacc ccgagtgcag
      721 cacgcagcag gggcacgaaa tctacgaccc ctgcgcgccg tattcccgcc tcggcgtaac
      781 caagcggaac tttcgatccg accttctgga cggcgtggag ggactgcact tccatacgct
      841 ctgcgagcag ggcgcggacg cgctgatcga aacgtttcat gcgtttgagc aatcgttcgg
      901 cgagtttttg ccgcgcatga agtggctcaa cctcggcggc ggacaccata ttacgaaacc
      961 aggatacgac gttgccgcgc ttgaggggct gattcgctcc attcgggaga aatacggcct
     1021 gacggtctat ctcgaacccg gcgaagcggt tgcgctaaac gcgggtgagc tggatacgac
     1081 ggtgctggat attgtggaaa acggcatgcc aattttgatt ttggatgctt ccgccgcctg
     1141 ccacatgccg gacgtgctgg aaatgcccta ccgcccgccg cttgccaatg cgggcgagcc
     1201 gaacgagaag gcctatacct gccgccttgc cgcgcggacg tgtctcgcgg gggacgtgat
     1261 cggcgactac agcttcgaca cgatgccgaa aatcggcgaa cggctgacgt tcggcgatat
     1321 ggcgatctac tccatggtga aaacgaacac cttcaacggc atgccgctgc ccgccatcgc
     1381 gctggaacat gcgaatgggg actgcgaact cgtaaagacg ttcggctatg aggatttcaa
     1441 agggaggctt tcgtaaggaa tgcgcatttc ttcgctgccc gcgcaggacg gtttccatat
     1501 gcccgccgag tttgaaccgc aggacggcgt tctgctggtt tggccgacgc gccccggaag
     1561 ctggggaaaa gacccccgcg cggcgcagga agcggtcacc gtccccgccg cgcccgtggt
     1621 gcgggag
//
LOCUS       DAOMGY010000444         1623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_89347_length_1623_cov_3.006378, whole genome shotgun sequence.
ACCESSION   DAOMGY010000444 DAOMGY010000000
VERSION     DAOMGY010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89347_length_1623_cov_3.006378"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLR57_07040"
     CDS             complement(<1..410)
                     /locus_tag="PLR57_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41248.1"
                     /translation="MSKQKASSALTGAGKKNQAREGAARILFLAAACVSILAVLLITV
                     FLLVNGFPAMAKIGVFKFLLGTTWRPGNDIYGILPMILGSLYVTAGALVLGVPIGLLT
                     AVFLSRFASPRMVKLLKPAVQLLAGIPSVVYGFFG"
     gene            complement(438..1373)
                     /locus_tag="PLR57_07045"
     CDS             complement(438..1373)
                     /locus_tag="PLR57_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQQ41249.1"
                     /translation="MKKVLAIALALLMVASLAACASTPAPEATEAPATEAAAADAPAA
                     EEPAAEMLDSDIVVVSREDGSGTRGAFIELMGVEQKDANGTKVDYTTLDAEIVNSTSV
                     VLTTVASNVDAIGYISLGSLNDTVKALEIDGAAATVENIKNGSYKVARPFNIVLGANV
                     SEAAQAFIDFIMSAEGQQVVADSHYIPMDNAPAYAGSSVSGKVVVAGSSSVTPVMEKL
                     KEAYMLVNPNAEIEVQMSDSSTGVNMAIEGSCDIGMASRELKDSETAQGVTSTVIATD
                     GIAIIVNLDNPISGLTTELVRAIYVGEITNWADAK"
     gene            complement(1403..>1623)
                     /locus_tag="PLR57_07050"
     CDS             complement(1403..>1623)
                     /locus_tag="PLR57_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41250.1"
                     /translation="CDQRIITKRKPYCKLRLFLRNEPIIRLFTLKLRGILPDKTQLYT
                     VKTLVFAILQRFDFSGILIKHTSIMLST"
BASE COUNT          334 a          424 c          481 g          384 t
ORIGIN      
        1 ccgaagaagc cgtacacgac ggacggaatg cccgccagca gctgcaccgc gggctttaag
       61 agctttacca tgcggggcga ggcaaagcgt gaaagaaaaa ccgccgtcaa tagtccaatc
      121 ggtacgccaa gcaccagcgc gcccgcggtt acatagagac tgccgagtat catggggaga
      181 atgccataga tatcgtttcc aggtctccag gttgtgccga gcaggaattt aaatacaccg
      241 atcttggcca tggcgggaaa gccgtttacc agcaggaata cggtaatcag caaaaccgcc
      301 agaatactca cgcaggcagc ggcgagaaaa agtattctcg ccgcgccttc gcgtgcttga
      361 ttttttttgc ccgcgcccgt gagggcggag gaggcttttt gcttactcat ccaaatctct
      421 tttcgatccg gtccgggtta tttcgcatcc gcccagttgg tgatttcgcc aacgtagatc
      481 gcgcggacaa gttccgttgt gaggccggag atcgggttgt cgaggttgac gatgatggcg
      541 ataccgtcgg tggcgatcac ggtggaggtt acgccctgcg cggtttcgct gtccttgagt
      601 tcgcgggacg ccatgccgat gtcgcagctg ccttcgatgg ccatgttgac gccggtgctg
      661 gagtcgctca tctggacttc gatttcagcg ttggggttca cgagcatgta agcttccttg
      721 agcttttcca tgaccggggt cacggaggag ctgccggcca caacgacctt gccggagacg
      781 gagctgcccg catacgccgg ggcgttgtcc atcgggatgt agtggctgtc cgcaacgacc
      841 tgctggcctt ccgcgctcat gatgaagtcg atgaacgctt gggcggcttc cgagacattc
      901 gcgccgagca cgatgttgaa cggacgggct actttgtagg aaccgttttt gatgttttca
      961 accgtggcgg ctgcgccgtc gatttcaagc gccttgacgg tatcattcag ggaaccgagg
     1021 gagatgtaac cgatggcgtc cacgttgctg gcaacggtcg tgagcacaac cgaagtgctg
     1081 ttgacgattt ccgcatccag cgtggtgtag tcaaccttcg tgccgttggc gtccttctgc
     1141 tcaacgccca tcagctcgat gaacgcgccg cgggtgccgc tgccgtcttc acgggagacg
     1201 accacgatgt cggagtccag catttcggcg gcgggttctt ctgccgcggg agcatcggct
     1261 gcggcggctt ccgtcgcggg ggcttcggtc gcttccggag cgggggtgga ggcgcaggcg
     1321 gcgaggctcg ctaccatgag cagagcaagt gcgattgcaa gtaccttttt catttcagaa
     1381 attctccttt tttctcattc atttatgtgc tcagcataat cgaggtatgt ttgatcaaaa
     1441 taccggagaa gtcaaatctt tgtaaaatcg caaaaacaag tgttttaact gtgtaaagct
     1501 gcgttttgtc aggtaaaatc ccgcgaagtt ttaacgtaaa aagcctaata atcggttcgt
     1561 tgcgtaaaaa cagccgcaac ttgcaatatg gctttctttt cgttataata cgttggtcgc
     1621 agt
//
LOCUS       DAOMGY010000445         1617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_89889_length_1617_cov_3.023047, whole genome shotgun sequence.
ACCESSION   DAOMGY010000445 DAOMGY010000000
VERSION     DAOMGY010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89889_length_1617_cov_3.023047"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PLR57_07055"
     CDS             <1..451
                     /locus_tag="PLR57_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQQ41251.1"
                     /translation="IMQPYVVDSIWHADGTDYSLVEQREPTEYRRILQQSTVDLIYPA
                     LLQVCVSGTARWLAKSFINKGPLTLGYTMAGKTGTAEINNDKSKELAWFICWRDNKGG
                     VPVTEEDARLVCIMLEVNLPLGDEWSQMKMDIARALLKDDTLNDTEG"
     gene            571..>1617
                     /locus_tag="PLR57_07060"
     CDS             571..>1617
                     /locus_tag="PLR57_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQQ41252.1"
                     /translation="MQTEAGTRRKKIVFTGGGTAGHVTPNIALMQQLLKEGWEVHYIG
                     SETGIEKELIGAMEGVSYHAIATGKLRRYFSWQNFIDPFRVLKGSFQARRIIRELQPD
                     VLFSKGGFVSVPVVMGAGKIPVVAHESDFSPGLSNRIASRFTDRVCVSFEDTLHYVPH
                     QKGVFTGTPIRPALYAGCRERALEFTGFSGEKPVLLVMGGSLGAQKLNELLRAALPKL
                     CETYDIIHLCGRGKKDCDCLACGYTQYEYIDQELPDLFALADVVLSRAGANAVFELLA
                     LNKPSVLVPLSKASTRGDQLLNARYFEKNGYALVLDQNTATPESLCLAIDEVYRDRER
                     YAAAMRNDTRADGTL"
BASE COUNT          347 a          501 c          467 g          302 t
ORIGIN      
        1 gattatgcag ccgtacgttg tggattccat ttggcacgcg gacggaacgg actactccct
       61 tgtggagcag cgtgaaccga ccgaataccg ccgcatcctg cagcagtcca ccgtcgatct
      121 gatctatccg gcgctgcttc aggtctgcgt cagcggcacc gcgcgctggc tggccaagag
      181 ctttatcaac aaaggcccgc tgacgctcgg ttatacgatg gccggcaaaa caggcaccgc
      241 cgaaatcaac aacgacaagt ccaaggaact cgcctggttc atctgctggc gggacaacaa
      301 gggcggcgtg cctgtgacgg aagaagatgc gcgcctcgtc tgcatcatgt tggaagtcaa
      361 tcttccgctg ggcgacgaat ggtcgcaaat gaagatggac atcgcccgcg cgcttttaaa
      421 ggacgacacg ctcaacgata ccgaaggttg acgccaattc cggttttccg gatggacaaa
      481 attcggttta ggacatacac tgaatctata acggactatg cacacgatcg gttcgcgccg
      541 gtcgtgtgtt tttcggggag aagaacaacc atgcagacag aagcaggcac cagaagaaag
      601 aagatcgtat tcaccggcgg cggcaccgcg ggccacgtta cgcccaacat cgcgctcatg
      661 cagcagctct tgaaagaagg ctgggaagtg cactatatcg gctccgaaac cggcattgaa
      721 aaagagctca tcggcgcgat ggaaggcgtt tcctaccacg ccatcgccac cggaaagcta
      781 aggcgttact tctcctggca gaacttcatc gacccgttcc gcgtgctcaa gggcagcttt
      841 caggcgcgca ggatcatccg cgaactccag ccggacgtgc tcttcagtaa gggcggcttt
      901 gtctctgttc cggtggtgat gggcgcgggc aaaatccccg tcgtggcgca cgaatcggac
      961 ttttcgccgg ggctgagcaa ccgcatcgcc tcccgtttta cagatcgcgt ctgcgtttcg
     1021 tttgaggata cgcttcatta tgttccgcat cagaaaggtg tgtttaccgg aacgcccatc
     1081 cgccccgcgc tgtacgcggg ctgccgcgaa cgcgcgctgg agttcaccgg attctccggc
     1141 gaaaagcccg ttctgctggt tatgggcgga agcctcggcg cgcaaaagct caacgagctg
     1201 ctccgcgcgg cgctgcccaa gctctgcgaa acatacgata tcatccacct ctgcggacgc
     1261 ggcaaaaagg actgcgactg ccttgcctgc ggctacacgc agtatgaata catcgatcag
     1321 gagcttcccg acctgttcgc gctggcggac gtggtgctct cccgcgcggg cgcgaacgcc
     1381 gtgttcgaac tgctcgcgct caacaagcct tccgtgctgg ttccgctgag caaggcctcg
     1441 acccgcggcg accagctgct caacgcgcgg tatttcgaaa agaacggcta cgcgctggtg
     1501 ctcgaccaga acaccgcgac gcccgaatcc ctctgcctcg ccatcgacga agtctaccgc
     1561 gaccgcgaac ggtacgccgc cgccatgcga aacgacacgc gcgcggacgg cacgctc
//
LOCUS       DAOMGY010000446         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_90501_length_1610_cov_2.989711, whole genome shotgun sequence.
ACCESSION   DAOMGY010000446 DAOMGY010000000
VERSION     DAOMGY010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90501_length_1610_cov_2.989711"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLR57_07065"
     CDS             complement(<1..790)
                     /locus_tag="PLR57_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQQ41253.1"
                     /translation="MAKDRVTFICGECGYESAKWMGKCPQCDSWNTMTESAQVKVKAA
                     APGSSVSQKLSKVTPLPQKRVSAGMEELDRVLGGGLLRGMAVLLGGDPGIGKSTLLLQ
                     AADALGREGIVLYASGEESAAQIKLRADRLGMKNDIDILCETDLEHIIDEAIAVKCNV
                     LIIDSIQTMYSEESAGAPGSVSQVRAATARLTRFAKDTGVVVFIVGHVTKDGNIAGPR
                     VLEHIVDTVLYFEGDRHEGLRLLRAVKNRFGSTNEVGVFEMCDNG"
     gene            complement(795..>1610)
                     /locus_tag="PLR57_07070"
     CDS             complement(795..>1610)
                     /locus_tag="PLR57_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41254.1"
                     /translation="GADENACDRFVRLDPETGAFESIEAERTNSTLRSPVENAEVLIY
                     ADVKADGGGTIRMINKATRENTALAEFTMGVPKLSLEAPYLVWMERTGDSSAKLMVCD
                     ITNGDLLTLAVFTDSTYADSAPSIKSGQVLYADADETNPGSSLIRTVLLSDNSRWDYA
                     AGSYVHDPESAGDRWAYLSGNHDGDSDLYVSIGGGSPMRVARGVIDFDITPSCVVFSR
                     DETVYAYVFSDNKTYVISETGTNSQFVMAGGDYALWRDMTDPENPLWKYIKVI"
BASE COUNT          284 a          511 c          469 g          346 t
ORIGIN      
        1 tgccgttgtc gcacatttca aacacgccga cttcgttggt tgagccgaag cggttcttta
       61 ccgcgcgcag gagccgcagg ccttcgtggc ggtcgccttc gaaatatagt acggtatcca
      121 caatgtgctc cagcacacgc ggccccgcga tgttgccgtc cttggtgaca tggcctacga
      181 taaacacgac cacgcccgta tcctttgcaa agcgggtgag ccgcgccgtt gccgcgcgta
      241 cctgactgac gctgccgggc gcacccgcgc tctcctcgct gtacatggtc tgaatcgagt
      301 cgataatgag cacgttgcac ttgacggcga tcgcttcgtc gatgatatgc tccagatcgg
      361 tttcgcagag gatgtcgata tcgtttttca tgccgagccg gtcggcgcgg agcttgatct
      421 gcgccgcgct ctcctcgccg gaagcgtaga gcacaatgcc ttccctgccc aacgcgtccg
      481 ccgcctgcag cagcagggtg gacttgccga tgcccggatc gccgcccaag agaaccgcca
      541 tgccgcgcaa taagccaccg cccagcacgc ggtcgagctc ttccatgccc gcggacacgc
      601 gcttctgcgg cagaggcgtc acttttgaaa gcttctggga gaccgaagaa ccgggagcag
      661 cggccttgac cttgacctgc gcggattccg tcatggtgtt ccagctgtcg cactgggggc
      721 acttgcccat ccacttggcg gactcgtagc cgcactctcc gcagataaac gtcacgcgat
      781 ctttggccat atgcttatat taccttgatg tatttccaga gcgggttttc ggggtcggtc
      841 atgtcgcgcc agagcgcgta gtcgccgccc gccattacaa actgcgaatt ggtgcccgtt
      901 tcgctgatga cataggtttt gttgtcggaa aagacgtagg cgtacaccgt ttcgtcccgg
      961 ctgaacacca cgcaggaggg cgtaatgtca aagtcaatca cgccgcgcgc tacccgcatc
     1021 gggcttccgc cgccaatgct gacgtagagg tcgctgtccc catcgtggtt gccgcttaag
     1081 taagcccagc ggtcgcccgc ggattccgga tcgtgcacat agctgcccgc ggcatagtcc
     1141 cagcggctgt tgtcgctcaa gagaaccgtg cggatcaggc tgctgccggg gtttgtctcg
     1201 tccgcgtcgg catacagtac ctggccgctc ttgatcgacg gcgcggagtc ggcatatgtg
     1261 gagtcggtga acaccgccag cgtaagcaga tcaccgtttg tgatatcgca gaccataagc
     1321 tttgccgaag aatcccccgt gcgctccatc caaaccagat acggcgcttc cagcgagagc
     1381 ttcggaacac ccatcgtgaa ctctgccagc gcggtgtttt cgcgcgtcgc tttattaatc
     1441 atgcggatgg ttccgccgcc gtccgctttt acgtccgcat agatgagcac ctctgcgttt
     1501 tcgacggggc tgcgcagggt gctgttcgtc cgctccgcct cgatgctttc aaaggcgccc
     1561 gtctcggggt caaggcgcac aaagcggtcg caggcgtttt cgtccgcgcc
//
LOCUS       DAOMGY010000447         1606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_90895_length_1606_cov_2.880722, whole genome shotgun sequence.
ACCESSION   DAOMGY010000447 DAOMGY010000000
VERSION     DAOMGY010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90895_length_1606_cov_2.880722"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLR57_07075"
     CDS             complement(<1..184)
                     /locus_tag="PLR57_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HQQ41255.1"
                     /translation="MTITLQSLRERASGLNRRVAVASAHDEDVLKAVIMAADAHICTP
                     TLFGDERRIRSLLGSLG"
     gene            complement(181..1239)
                     /gene="buk"
                     /locus_tag="PLR57_07080"
     CDS             complement(181..1239)
                     /gene="buk"
                     /locus_tag="PLR57_07080"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HQQ41256.1"
                     /translation="MKILVLNPGATSTKIAVYENETEVFCETIRHTQEELAGFSHVID
                     QLDFRRELVMETLRTHGFSRADFAAVCSRGGLLKHIPSGTYRISDAVLQDIYHPPYGE
                     HASSLGPVIAKQLADLAGIPAYLVDPVSVDEMIPLARVSGYKGMERESFFHALNQKAV
                     ARKASAQLGKAYEEVNLIVAHMGGGVSVAAHQKGRVIDLFNVKDDGSFSMDRGGALPV
                     NAIIRLCFSGLSQQEIKRRLGTESGVFSYLGTRDFREVEARMLSGDEQATLIFKAMAY
                     QHAKDIGALAAVLRFNVDAIVLTGGIAFSSRFCDELSGYVQKIAPIIRIPDEEEMRAL
                     AEGALRVLSGEPAKEYPA"
BASE COUNT          348 a          437 c          489 g          332 t
ORIGIN      
        1 cgccgagcga accgagcaga gaacggattc tgcgttcgtc gccaaagagc gtcggcgtgc
       61 agatatgcgc gtcggctgcc atgatgacgg ccttgagcac atcttcgtcg tgcgcactgg
      121 caaccgccac gcggcggttc agcccgcttg cgcgttcacg gagcgattga agggtaatcg
      181 tcatgcaggg tactcctttg ccggttcgcc gctgagcacc cgcagcgcgc cttcggcgag
      241 cgcgcgcatt tcctcttcgt cgggaatgcg gatgatgggg gcgatttttt gaacatagcc
      301 ggaaagttcg tcgcagaacc ggctggaaaa tgcgattccg ccggtaagca cgattgcgtc
      361 tacgttaaag cgcagcacgg cggcaagcgc gccgatatcc tttgcgtgct gatacgccat
      421 ggctttgaag attaacgttg cctgctcgtc gccggagagc atgcgggctt ccacctcgcg
      481 gaagtcccgc gtaccgagat acgaaaacac gccggattcc gtgccgagac ggcgcttgat
      541 ctcctgctgg ctcagcccgg aaaaacagag cctgattatc gcgttgaccg gcagagcgcc
      601 gccgcggtcc atggaaaagc tgccgtcgtc cttgacgttg aacaggtcga tcacccggcc
      661 cttttggtgc gcggcaacgg agacgccgcc gcccatatgc gcaacgatca ggttgacttc
      721 ctcataagct ttgccaagct gtgcggaagc tttgcgcgcg accgctttct ggttgagcgc
      781 gtgaaaaaag ctctcgcgct ccatgccctt atagccggaa acacgcgcta gcggaatcat
      841 ttcgtccacg gaaacgggat cgaccagata ggcgggaata cccgcgaggt cggcgagctg
      901 tttggcgatg actgggccga gcgaggacgc atgttcgccg tagggcggat ggtagatatc
      961 ctgcagcacc gcgtcgctga tgcgatatgt gccggaagga atgtgcttga gcaaccctcc
     1021 gcggctgcag acggcggcaa agtccgcgcg ggaaaatccg tgcgtgcgga gcgtctccat
     1081 caccagctcg cggcgaaaat cgagctggtc gatcacatgc gaaaaccctg cgagctcctc
     1141 ctgcgtatgc cggatggtct cgcaaaagac ttccgtttcg ttttcgtata cagcgatctt
     1201 ggtggaggtt gcacccggat tgagaacgag gattttcatg ttcgggcctc cgatatggaa
     1261 ttgcgtttgc aggaaaactt tgtgaaataa tcaaaacaaa cagtcattac attactatcg
     1321 taggttttcc cgggtggagt gtcaatgcgc ggcggacaaa tatatagtat acttgtttta
     1381 ggtatgcatt ttaggcggcg agatacggcc agcgtgtgcg cggcatgaaa ggttgtaatc
     1441 atgtccgccg cggtcaagaa accaaatcga agttgaggta ttcttccttc atgaagcagt
     1501 catccgaaca gcggatacca gcggaacaga agatcgaagc ggtgagaacc atgcaggagt
     1561 gcatccggat gaacgccgcc caatcggact ttgcgccgga ggacgt
//
LOCUS       DAOMGY010000448         1605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_90973_length_1605_cov_2.979355, whole genome shotgun sequence.
ACCESSION   DAOMGY010000448 DAOMGY010000000
VERSION     DAOMGY010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90973_length_1605_cov_2.979355"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLR57_07085"
     CDS             complement(<1..445)
                     /locus_tag="PLR57_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HQQ41257.1"
                     /translation="MEAIQHVFQQRQHMLLEDYEIYHYKDSRELHVSLHFHDFYECYL
                     LLSGTLRYQIESASFSIQPGDLLLIGPNQLHRPLFTQTDEPYERLVLWLSRSFVERLS
                     TPETDLAACFSAQRLSANRLSAEQHEAITRQFFALLNATNSEQFGR"
     gene            654..>1605
                     /locus_tag="PLR57_07090"
     CDS             654..>1605
                     /locus_tag="PLR57_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQQ41258.1"
                     /translation="MSAKPRYAIACPTSMGVRMTPEDRRSVQVSNRYLLQSTSAESNV
                     LNIASSLGYECLVLTKFIEGSPISQFIQGELRARNIRYEGASVPQGGPWGYRHQFNIA
                     DSGFGLRGPRVWNDRTGEVGRTLSIDEFDIERIFGEEGVGILHLSGLIAALSPETAAF
                     CAELAKRAKSYGTKIAFDLNYRASFWKGREEELSQVFRSIAGLADILVGNEEDFQLAL
                     GIQGPEAGGKDLAAKIEGFKGMIGEAKKAYPNAKFFATTLREVVNANEHLWGAILSAG
                     DEWFVEEPRKIPVLDRIGGGDGFTGGLLYGVYRGFSPEQCL"
BASE COUNT          355 a          436 c          494 g          320 t
ORIGIN      
        1 cccgcccgaa ctgctcgctg ttggttgcgt tgagcagggc aaagaactgc cgtgtaatcg
       61 cctcgtgctg ctcggcggag aggcggtttg cgctgaggcg ctgcgcggaa aagcaggcgg
      121 cgaggtccgt ttccggcgtg gagagacgct cgacaaacga gcgggaaagc cagagaacga
      181 gccgttcgta gggttcgtcc gtctgcgtaa aaagcgggcg gtgcagctgg ttcgggccta
      241 tgagcagcag atcccccggt tgaatggaga acgacgcgct ctcgatttgg tagcgaagcg
      301 tgccggagag cagcaggtaa cactcgtaaa agtcgtggaa atgcagcgaa acgtgcagtt
      361 cccggctgtc tttgtaatgg tagatttcgt agtcctccag aagcatgtgc tggcgctgct
      421 gaaagacatg ctgaatggct tccataatcg ttctccttat actgccagta tagagcaata
      481 ttcgcaatgt ttcaagcaag atatgtatag aacctgtcga atcctgctga tatgataagg
      541 ctgtcgatca atcgctcccc atccggtcat ttggatcaaa aacgacgttc taccaaacgt
      601 aaaacgaaca gattacgaaa atgaaataga acaaaataga aagaaggatc gctatgtccg
      661 ccaaaccccg ttacgccatc gcctgcccca cgagcatggg cgtgcgcatg accccggaag
      721 accgcagaag cgtgcaggtg agcaatcgct acctgctgca gagcacgagc gcggagtcca
      781 acgtgctcaa tatcgcaagc tcgctcggct acgagtgcct tgttttgact aagtttatcg
      841 agggcagccc catttcgcag ttcattcagg gcgagttgcg ggcgcgcaac atccgttacg
      901 agggcgcaag cgttccgcag ggtgggccct ggggttaccg ccaccagttt aacattgccg
      961 acagcgggtt cggccttcgc gggccgcgcg tctggaacga ccgtacgggc gaagttggac
     1021 gcacgctttc gattgacgaa ttcgatattg aacggatctt cggcgaggag ggcgtgggaa
     1081 tcctgcatct ctccggcctg attgccgcgc tctcgccgga aaccgccgcg ttctgcgcgg
     1141 agctggccaa gcgcgccaaa tcttacggta cgaagatcgc cttcgacctc aactatcgcg
     1201 cttccttctg gaaggggcgc gaagaagagc tttcccaagt gttccgcagc atcgcagggc
     1261 tggcggatat tctcgtgggc aacgaggaag atttccagct cgcgctcggc attcaggggc
     1321 cggaagcggg cggcaaggat ctcgccgcca agatcgaagg ctttaagggc atgatcggag
     1381 aagcgaaaaa ggcgtatccg aacgcaaagt tctttgccac gacgctgcgc gaagtcgtca
     1441 acgcaaacga acacctctgg ggcgccattc tctccgcggg cgacgagtgg ttcgttgagg
     1501 agccgcgcaa aatccccgtg ctcgaccgca tcggcggcgg ggacggcttt acgggcggct
     1561 tgctctacgg cgtgtatcgc ggtttttcgc cggaacagtg tctgc
//
LOCUS       DAOMGY010000449         1603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_91149_length_1603_cov_2.963178, whole genome shotgun sequence.
ACCESSION   DAOMGY010000449 DAOMGY010000000
VERSION     DAOMGY010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91149_length_1603_cov_2.963178"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..638)
                     /gene="raiA"
                     /locus_tag="PLR57_07095"
     CDS             complement(99..638)
                     /gene="raiA"
                     /locus_tag="PLR57_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQQ41259.1"
                     /translation="MRISITGKNLEISDYLNDLVEKKVGKLERYFPQDTEVQVTLSVE
                     KSRHIVEVTIPYQGGVIRGEEITGDMYASIDNVLDKLEKQIIRHRTKLEKCLRSGAFR
                     YSEPLYGGSYEEADEDEGPKIVRVKRFAIKPMSEEEAMLQIEMVGHSFYVFINSETNA
                     MNVLYKRKDGNYGLIEPEL"
     gene            complement(829..>1603)
                     /locus_tag="PLR57_07100"
     CDS             complement(829..>1603)
                     /locus_tag="PLR57_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014120312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ41260.1"
                     /translation="QDTTEESERETLRWLEESARFTRVKPILTPRFTPSCTNEILDWL
                     GKLSQERGLCVQSHLSENRAEIELVKQLHPDCRQYWETYEKYGLWKEKTIMAHCVHSD
                     EREQDAMIRHNVLCVHCPDSNINICSGFTPVRKMAERGVWLALGSDIAGGAQLPMLQV
                     LTGALRMSKARAIATEGAEPFLTVGEAYYLATSAGAKYFGDKEGFAPGNRLHAVVIDD
                     SEFPESTRTLTTKERFERAVYLAGAQDIVAVYGDGERIR"
BASE COUNT          294 a          457 c          441 g          411 t
ORIGIN      
        1 gcgggtggtt ttttatttct cccgcttcgc ggtcgaaacg gactgaagtc actaaaaaag
       61 agaccgggcg tttgcccggt ctcttgtgct tctatctctt acagttccgg ctcgattaag
      121 ccgtagttgc cgtctttgcg cttgtagagc acgttcatcg cgttcgtttc cgaattgatg
      181 aatacgtaga acgagtgccc gaccatttca atctgcagca tcgcttcctc ttcgctcatc
      241 ggcttgatgg caaagcgttt gacgcgcacg atcttcgggc cttcgtcctc atccgcttcc
      301 tcataggaac cgccgtagag cggctcgctg tagcggaacg caccgctgcg gagacacttt
      361 tccagcttgg tgcggtggcg gatgatctgc ttttccagtt tgtccagaac gttgtcgatg
      421 gaggcgtaca tgtcccccgt gatctcctcg ccgcgaatca cgccgccttg gtaaggaatc
      481 gttacttcaa cgatatgacg gctcttttca acggagaggg ttacctgcac ttcggtgtcc
      541 tgcggaaagt accgttccag tttgcctact ttcttttcca caaggtcgtt caggtaatcg
      601 gagatctcta ggtttttccc ggtaattgaa atacgcatgt taccactcct tctccggcgg
      661 attctcaatt gaattcgcct attgttatta ttcgatggaa agatgtgttt tccctgccgg
      721 aaaggtgaac gaattcgacc aaaccgtgag gaaattttgc gtgtctcgaa tctgccgcgc
      781 gccggacgtt ttacggcgtt tgttcagcgg caaaataaca gcgggcggtc agcgaatccg
      841 ctcgccgtcg ccatatacgg caacaatgtc ctgcgcgcct gccagataca ccgcgcgttc
      901 gaagcgttcc tttgttgtca gcgtccgcgt gctctccggg aactcgctgt cgtctataac
      961 caccgcatgc aggcggttgc cgggagcaaa gccttccttg tcgccaaagt acttcgcgcc
     1021 agcgctggtg gcgaggtaat acgcttctcc gacggttaaa aacggctccg cgccttccgt
     1081 tgcaatggct ctggccttgg acatgcgcag ggctcccgta agcacctgca gcatgggcag
     1141 ctgcgcgccg cccgcgatat ccgaaccaag cgcgagccaa acgccgcgtt ccgccatttt
     1201 gcgcacgggc gtaaatccgc tgcagatgtt gatgtttgag tccgggcagt gcacgcagag
     1261 gacgttgtgc cgtatcatcg cgtcctgctc gcgctcgtcg gaatgcacgc agtgcgccat
     1321 gatcgttttt tctttccaca gcccgtattt ttcatacgtc tcccagtact gccggcagtc
     1381 gggatgcagt tgtttgacga gctcaatctc cgcgcggttc tcggaaaggt ggctctggac
     1441 acagagtccg cgctcctgcg aaagtttgcc gagccagtcc agtatttcgt tggtgcagga
     1501 aggcgtaaaa cgcggggtca gaatcggttt gacgcgggta aaccgcgcgg attcttccag
     1561 ccagcgcagc gtttcgcgtt cggattcctc ggtggtgtcc tgg
//
LOCUS       DAOMGY010000450         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_91606_length_1598_cov_2.667531, whole genome shotgun sequence.
ACCESSION   DAOMGY010000450 DAOMGY010000000
VERSION     DAOMGY010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91606_length_1598_cov_2.667531"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLR57_07105"
     CDS             <1..516
                     /locus_tag="PLR57_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQQ41261.1"
                     /translation="ERAFGFVDVLVNNAGIALPNQLVTETSAADWSRVFGVNMDGMFF
                     VTRAVLPSMVSNQRGSIVNVSSMWGVTGGSCEVAYSASKAAVIGFTKSLAKEVAPSGI
                     RVNCVAPGFVSTEMTAGLDAETVEAIREETPLLKLGTPEDIASAVLFFALEGASFITG
                     QTLSVDGGRCI"
     gene            662..737
                     /locus_tag="PLR57_07110"
     tRNA            662..737
                     /locus_tag="PLR57_07110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:695..697,aa:Arg,seq:acg)
     gene            843..>1598
                     /locus_tag="PLR57_07115"
     CDS             843..>1598
                     /locus_tag="PLR57_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HQQ41262.1"
                     /translation="MLTIYDWFGYELPDEQRYRLIKEAGFDGVLLWWSEYLNRGDYRG
                     GLRLAREEGLFVENIHTPFQVQDGLLLDTLEGETTMQCYLECIADCAAFEIPTLVVHL
                     PDDDKPHTALGLNRIRKMAGLAERLSVNIALENLSNFENLSFVLQTVDSPRVGFCYDC
                     GHHYRCYPNIDLLALFGSRCMALHLHDFEGSSMHRLPFDGVIDWPDAMKRITESGYRG
                     ATAIEAMNWEYGTLSAEAFLQEAVLAAQKLEQMR"
BASE COUNT          344 a          401 c          489 g          364 t
ORIGIN      
        1 gagcgcgcgt tcggctttgt ggacgtattg gtcaacaacg cgggtatcgc tctgccgaac
       61 cagctcgtga ccgaaacgag cgccgcggac tggtcgcgcg tgttcggcgt gaatatggat
      121 ggcatgttct ttgtcacccg tgcagtattg cccagtatgg taagcaatca gcgcgggagc
      181 atcgtcaacg tttcgtccat gtggggcgtg accggcggct cctgcgaagt ggcgtattcc
      241 gcttccaaag cggcggtgat tggctttaca aagtcgctgg caaaggaggt tgcgcccagc
      301 ggaatccggg tcaactgcgt ggctcccggc tttgtgtcta cggagatgac cgcgggtctc
      361 gacgcggaaa cggtggaagc catccgtgaa gaaacgccgc tgttaaagct cggtactccg
      421 gaggatatcg catccgccgt gctgtttttt gcgctcgaag gcgcttcgtt catcacgggg
      481 cagacgctca gcgtggacgg cgggcggtgc atctaggcgg ctgtggatat tgtaccataa
      541 tcgatcgcgc tttcgaaaac ggacgaaaaa atcgtcgcag tcagagctgt ggaatcgctc
      601 gaaaaggctt gacattccac cgccttacgg ctagaataaa tgagcacgca aaaagtgctt
      661 tgcgcttgta gctcagtgga tagagtgccc ggctacgaac cggtaggtcg caggttcaaa
      721 tcctgccagg cgcgccaaca aactccatgt atcggatttc gatctgtgca tggagttttg
      781 ttttattttg acggattctg tgctacaact agatcagact tttttaaagg attcaaaaaa
      841 ttgtgctaac gatttacgac tggttcgggt atgagttgcc cgacgagcag cggtaccggc
      901 ttataaaaga agcaggcttt gacggcgtgc tgctttggtg gagcgagtat ctcaaccggg
      961 gcgactatcg cggcggcctg cgtcttgcgc gcgaagaggg gctttttgtg gagaatatcc
     1021 atacgccgtt tcaggtgcag gacggactct tgctggacac gctggaaggc gaaacgacaa
     1081 tgcaatgcta cctcgagtgc attgcggact gcgccgcgtt tgagattcca acattggtgg
     1141 tgcatctgcc ggacgacgac aaaccgcata ccgcgctggg gctgaaccgc atacggaaaa
     1201 tggcgggact ggcggagcgg ctctccgtca acatcgcgct ggaaaatctg agcaactttg
     1261 aaaacctgtc gtttgttctg caaacggtgg actcaccgcg cgtcggattc tgttacgatt
     1321 gcgggcatca ctaccgctgc tatccgaaca tcgacttgct cgccttgttc ggttcccgct
     1381 gcatggcgct gcatctgcac gattttgagg gaagttccat gcatcgactg ccctttgacg
     1441 gcgtaataga ctggcctgat gcgatgaagc gaatcacgga atcgggttat cgcggcgcaa
     1501 cggcgattga agcgatgaac tgggaatacg gcacgctttc cgcggaagcg tttctgcaag
     1561 aagcggttct tgcggcacaa aagctggagc agatgaga
//
LOCUS       DAOMGY010000451         1596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_91761_length_1596_cov_3.545101, whole genome shotgun sequence.
ACCESSION   DAOMGY010000451 DAOMGY010000000
VERSION     DAOMGY010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91761_length_1596_cov_3.545101"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLR57_07120"
     CDS             complement(<1..371)
                     /locus_tag="PLR57_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41263.1"
                     /translation="MQNALYIEVSVVGVALLAIILANQSKSVGSSSLQRQFNRMIYAS
                     IGILIIDAACWVIDGTTFPGARTANFIIETLYYFFNIFMPFLWAIYTENALSADSRRV
                     RRRMHLLAFPLAVYIVLLFLN"
     gene            complement(626..>1596)
                     /locus_tag="PLR57_07125"
     CDS             complement(626..>1596)
                     /locus_tag="PLR57_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020064864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQQ41264.1"
                     /translation="TPNPTKSEVMAGAALAREHGCDFVIGFGGGSSIDAGKAIAVMAT
                     NPGDYWDYISGGTGKGKPVPNDPLPIIAITTTAGTGTEADPWTVVTKEDTNEKIGFGY
                     DKTFPVLSIVDPELMLTVPPALTAYQGFDALFHSTEGYLNNIAYPISDAFALRAISLV
                     GKSLARAVNNGSDLEARTDVALANTLSGLVESTSGCISEHSLAHAMGALNPEIPHGAA
                     LISISAAYYTHFANSGKCDARLIDMARALGKADAKTPMDFVAALVELQKACGVDAIKM
                     SDYGIRYEDLPKYVENAFDTMGGLFQFDPSALSRADVLAIFQASYR"
BASE COUNT          371 a          430 c          459 g          336 t
ORIGIN      
        1 atattgagaa acagcagcac gatataaacc gcaagaggga atgccaaaag gtgcatgcgg
       61 cggcgtacgc gtctggaatc cgcagacagc gcgttctcgg tataaatcgc ccataggaac
      121 ggcataaaga tattgaagaa gtaatacagc gtttcaatga tgaaattcgc tgtccgtgca
      181 ccgggaaacg tcgtgccgtc gataacccag caggccgcgt cgatgatgag gataccgatg
      241 ctcgcataga tcatgcggtt gaactgccgc tgcagggaag aagagccgac ggacttgctc
      301 tggttggcga gaattatcgc caaaagagca acgccaacaa cacttacctc gatatataac
      361 gcgttttgca tccgcatcct ccgagaatga ttgttcttgc gcgaacccgc cgcatattta
      421 aaggatcaaa tcaataataa attccgaatt tattacagta taacatagat tcttcgtcaa
      481 aaaaaccagc gaattcctac gaaaacagcc acttttcagg ttctttacag caattgctgc
      541 agtgctgcat aatcaagagg cgacggaaaa agcccctttg ccgtcaagac aaaagggctt
      601 tgtttgtaaa aataaatcaa ttcgctcagc ggtaggaagc ctggaagata gccagcacgt
      661 ccgcgcggga aagtgccgag gggtcaaact ggaacagccc gcccatcgtg tcgaaggcgt
      721 tctcgacata cttcggcaaa tcttcgtagc gaatgccgta atcgctcatc ttgatcgcgt
      781 ccacgccgca agctttctgg agttctacca gtgctgcaac aaaatccatc ggcgtttttg
      841 cgtccgcttt gccgagcgcg cgcgccatgt cgatcaaacg cgcatcgcac ttgccggagt
      901 tggcaaagtg cgtgtaatac gccgcgctga tgctgatgag cgccgcgccg tgcggaatct
      961 ccgggttgag cgcccccatt gcgtgcgcaa gggagtgctc ggagatgcag ccgctcgtgc
     1021 tttcgaccag ccccgaaagc gtgttcgcca gcgcaacgtc cgtccgcgct tccagatcgc
     1081 tgccgttatt gaccgcacgg gcaagggatt tgccgacaag cgaaatggcg cggagagcga
     1141 acgcgtcgct gatcgggtaa gcgatgttgt tgaggtagcc ttccgtactg tggaagagcg
     1201 catcgaaccc ctgatacgcc gtcagcgcgg gcggcacggt gagcatgagc tcgggatcga
     1261 caatcgatag cacggggaac gttttgtcgt agccaaagcc gatcttttcg ttggtgtctt
     1321 ccttggtgac gaccgtccaa gggtcggctt ccgtgccggt gcccgcggtg gtggtaatcg
     1381 caataatggg gagcgggtcg ttggggacgg gtttgccctt gccggtgccg ccggagatgt
     1441 agtcccagta gtcgccgggg tttgtcgcca ttacggcgat ggccttgccc gcgtcgatgc
     1501 tgctgccgcc gccaaagcca atcacaaaat cgcagccgtg ctcgcgcgca agcgccgcgc
     1561 ccgccatgac ttcgctcttg gtggggttcg gtgtaa
//
LOCUS       DAOMGY010000452         1596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_91771_length_1596_cov_3.064893, whole genome shotgun sequence.
ACCESSION   DAOMGY010000452 DAOMGY010000000
VERSION     DAOMGY010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91771_length_1596_cov_3.064893"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLR57_07130"
     CDS             complement(<1..483)
                     /locus_tag="PLR57_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQQ41265.1"
                     /translation="MSRLFSLFQNPLQLLYILPAILIGMTVHEWAHAFAAYKLGDPTA
                     KNLGRMTLNPIAHFDIFGFLCLLLVGFGWAKPVPINSRNFKHFRRDDIIVSLAGITMN
                     LIVAFLFSFIYVLGFTKCGLGTNEAFTSIFGSILSINLALAIFNLLPIYPLDGSHVLE
                     S"
     gene            complement(681..>1596)
                     /locus_tag="PLR57_07135"
     CDS             complement(681..>1596)
                     /locus_tag="PLR57_07135"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HQQ41266.1"
                     /translation="MSDALDRYYADRDSIVRIRRHGSDLRKVVSNALSRAQNKYAAFQ
                     EAIAQTGQYEEARINGELTLANLHLFRPGASELVVDDYFADPPAKRVIPLDSALSAQE
                     NAKKFFKQYRKGKLGKAYAESQVEGLVVEIDYLEGQLENIEKCDTLYELYEIKDELIR
                     EKYLRPEKARSPKPGNKASAPMRFVSSDGITILIGKNNRQNDALTLQTAKSDNLWLHA
                     KNIPGSHVIIDFEGEPPEQTLKEAAALAVYYSQARLSSGVPVDYTPRRFIKKPSGARP
                     GMVVYSTNRTLYVTPDAALIQRLKAQSE"
BASE COUNT          327 a          412 c          482 g          375 t
ORIGIN      
        1 actttcgagc acatgcgaac cgtccagcgg atagatcggc aggaggttga agatggccaa
       61 agccaggttg atcgagagga tcgaaccgaa aatgctcgta aacgcttcgt tcgtgccgag
      121 tccacacttg gtaaagccaa gcacatagat aaacgaaaac aggaatgcga cgatgaggtt
      181 catcgtaatg cccgcgagag aaacgattat atcgtcccgg cggaagtgct tgaagttgcg
      241 gctgttgatc ggcacgggtt ttgcccagcc gaagccaacc agcagcaggc agagaaagcc
      301 gaagatatcg aagtgcgcaa tagggttgag cgtcatgcgc ccaaggttct tggcggtcgg
      361 gtcgcccagc ttgtaggcgg caaacgcgtg cgcccactcg tgcaccgtca tgccgatgag
      421 gatggcaggc aggatgtaaa gaagctgcag cgggttttga aatagtgaaa ataagcgtga
      481 caattcgctc aatctcctta aaattcattc gtttccggcg cgggcagggg atgccggttc
      541 aagccttgcg ccggtttcat tatacgggaa acggtgcatg cgcgcaactc atgcgcggcg
      601 ttgacggacg tttgccacag aatgttcacg cgggtttcat gcgtgttttc cgcttttggt
      661 cggtgaaaat tggaacaaaa ctactcgctc tgcgctttca gccgttgaat cagcgctgcg
      721 tcgggggtaa cgtacagcgt gcggttggtg gaatacacca ccatgccggg acgtgcgccg
      781 gaaggcttct ttatgaacct tctcggcgtg tagtccacgg gaacgccgct tgacaagcgt
      841 gcctgcgagt agtaaaccgc gagcgcggcc gcctctttga gcgtttgctc cggcggctcg
      901 ccttcgaaat cgatgatcac gtgcgaaccg gggatgttct ttgcgtgcag ccagagattg
      961 tccgactttg cggtctgaag cgtcagcgcg tcgttctggc ggttgttttt tccaatcaga
     1021 atggtgattc cgtcgctgga aacgaaccgc atcggcgcgg aagccttgtt gccgggtttc
     1081 ggggaacgcg ctttttccgg tcgcaggtat ttttcgcgga tcagttcgtc cttgatttcg
     1141 taaagctcgt agagcgtgtc gcacttttcg atgttttcta gctggccttc gagataatcg
     1201 atttcgacga cgaggccttc cacctgcgat tcggcgtatg ctttgccgag cttgcccttt
     1261 cgatactgct taaagaactt ctttgcgttc tcctgcgccg agagcgcgct gtcgagcgga
     1321 atcacgcgtt ttgccggagg gtcggcaaaa tagtcgtcca cgacaagttc gcttgcgccg
     1381 gggcggaaga gatgcaggtt cgccagcgtg agttcgccgt tgatgcgcgc ttcttcgtac
     1441 tgacccgttt gcgcgattgc ttcctgaaac gcggcgtact tattctgcgc gcggctcaga
     1501 gcgttggaaa cgacttttcg caggtcggaa ccatgcctgc ggatgcgaac aatcgaatcc
     1561 cggtcggcat agtagcgatc cagcgcgtcg ctcatc
//
LOCUS       DAOMGY010000453         1594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_91942_length_1594_cov_3.404808, whole genome shotgun sequence.
ACCESSION   DAOMGY010000453 DAOMGY010000000
VERSION     DAOMGY010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91942_length_1594_cov_3.404808"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLR57_07140"
     CDS             <1..213
                     /locus_tag="PLR57_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQQ41267.1"
                     /translation="PDVIVTTIPSSEDGTAGVNEILARVGWESVQAIADKKVFYIDGE
                     LLSRPGPRLLDVVNALYVFLYETPAE"
     gene            complement(385..1503)
                     /locus_tag="PLR57_07145"
     CDS             complement(385..1503)
                     /locus_tag="PLR57_07145"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQQ41268.1"
                     /translation="MQKKDPPILVTRPSLPPVEEYEAMVREIFGSGYLTNGGAMHKRL
                     EAALAEKLNAPYVRLFVNGHLALENALQAFGLEKGEIVTTPFTFISTTHAIVRSGFEP
                     VFCDVEKDYYTLDPMLLEQAITPRTRAILPVHVYGNPCDMAAIERIAAKHGLPVLYDA
                     AHAFGVTINGRGVAEFGTASMFSFHATKSFHTIEGGAVACADEAFADKLNHLKNFGIY
                     GEEITQVGGNAKMNEFQAAMGLCNLRYFDREIEKRAKVVARYRENLKGIEGVSLRPVP
                     AGVKSNFAYFPATFDPEAFGCTRDDVVKTLNEYGIFPRKYFYPLTSAFACYKGRFNPK
                     DTPVALRASERVVTLPLYADLALSDVDDICKIIASCRK"
BASE COUNT          317 a          466 c          454 g          357 t
ORIGIN      
        1 ccggacgtta tcgtgaccac gattccgagc agcgaggacg gtacggcagg cgttaacgag
       61 attctcgcgc gcgtcggctg ggaaagcgtt caggccattg cggacaagaa ggtattctat
      121 atcgacggcg agcttctctc ccgccccggc cccaggcttt tggacgttgt gaacgcgctg
      181 tatgtattcc tttatgaaac gcccgccgag taattggctg tagggtaata taaaaacgct
      241 tcggagactt ccgaagcgtt ttttgatgga ttgtattttg agggctccgc cctcacccgc
      301 caaggggcac gccccttgga accccatgcg caggcatatc tgcaagtttt agtaattaca
      361 aaagaatcgg acgacagaac gttgctactt tcgacaagat gcgatgattt tgcagatgtc
      421 gtccacgtcc gagagcgcca gatccgcata cagcggcagc gtgaccacgc gttcggacgc
      481 cctgagcgcc accggcgtat ccttggggtt gaacctgccc ttgtagcagg cgaaagcgct
      541 cgtgagcgga taaaaatact tgcgcggaaa gataccgtat tcgttgagcg ttttaaccac
      601 gtcgtcccgc gtgcagccga acgcttccgg atcgaatgtt gcggggaagt acgcaaagtt
      661 actcttgacc cccgccggaa ccgggcgcag ggaaacgcct tcgatccctt tgaggttttc
      721 gcggtaccga gcgacgacct ttgcgcgctt ttcgatttca cggtcgaaat atcgcaggtt
      781 gcacaagccc atcgccgcct gaaactcgtt catcttggcg ttgccgccca cttgcgtgat
      841 ctcctcgccg tagatgccga aattcttgag gtggttgagc ttgtccgcaa acgcttcgtc
      901 ggcgcaggcg accgcgccgc cttcgatggt atgaaagctc tttgttgcgt ggaagctgaa
      961 catagaagcc gtgccgaact ccgcaacgcc cctgccgttg atcgttactc caaaggcatg
     1021 cgccgcgtcg tacagcaccg gcaggccgtg tttggccgcg atgcgctcga tggccgccat
     1081 gtcgcagggg ttgccgtaga catgcacggg cagaatggcg cgtgtgcgcg gcgtaatcgc
     1141 ctgctccagc agcataggat cgagtgtgta gtagtccttc tccacgtcgc agaacacggg
     1201 ctcgaacccg ctgcgcacga tggcgtgcgt ggtggagata aacgtaaacg gcgtggtaac
     1261 gatctcccct ttttccaacc cgaacgcctg cagagcgttt tccagcgcga ggtggccgtt
     1321 gacaaacaaa cgcacgtacg gcgcgttcag tttttccgcc agcgcggctt cgagacgttt
     1381 atgcatcgcg ccgccgttgg tcagatagcc gctgccgaag atttcacgca ccatcgcttc
     1441 atactcctcc accggcggga gggacggccg cgtgaccaga atcggcgggt ctttcttctg
     1501 catactgctc cttttcgcct tcctgtttga aagcgatata tcttttttat ggggactccg
     1561 tccccaaacc cctgctcaag gcttaggcag ccct
//
LOCUS       DAOMGY010000454         1592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_92117_length_1592_cov_3.325309, whole genome shotgun sequence.
ACCESSION   DAOMGY010000454 DAOMGY010000000
VERSION     DAOMGY010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92117_length_1592_cov_3.325309"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLR57_07150"
     CDS             complement(<1..528)
                     /locus_tag="PLR57_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41269.1"
                     /translation="MVTVKRSSKHLKYTPSRRRWNLDATSFGPALKLVLALVLLAAFA
                     LLTIYVIVPFITARVQSGEPLFSNEPTESVAVETAPPKNPILSNTVKTVQYGEGYGLP
                     TAVVDPSVFDGKILFATGADENACDRFVRLDPETGAFESIEAERTNSTLRSPVENAEV
                     LIYADVKADGGGTIRM"
     gene            complement(666..>1592)
                     /locus_tag="PLR57_07155"
     CDS             complement(666..>1592)
                     /locus_tag="PLR57_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41270.1"
                     /translation="VVEDETIAVLFRQASENPQDADALAAAYLAEHDVKAYAAMKVDP
                     ERLKALADASGLIEGKLSLLLQIELEGSATAVPTTVLEADIGSIPVDAVTYRTFVKQT
                     AGNPDSAEWEGETILTYFDDSGVDKSTYGYNVYTNRVLSLDGLKMKALSVEVDATGIK
                     NLQVEITFPSGWTEEEIQAFSGTYGMQFQLLINGETGEWTVSGVMRSLDAAEPSKRVW
                     HCRQAYGVPLGLLPEIDELALIPYISYCTAYYELSPDQAGELVVRGKTTPLNLDEPFS
                     LWNEYYGGFDTSTSYYPQYAVSFPIPSAANKP"
BASE COUNT          317 a          465 c          450 g          360 t
ORIGIN      
        1 catgcggatg gttccgccgc cgtccgcttt tacgtccgca tagataagca cttcggcgtt
       61 ctcgacgggg ctgcgcagtg tgctgttcgt ccgctccgcc tcgatgcttt caaaggcgcc
      121 cgtctcgggg tcgagacgca caaagcggtc gcaggcgttc tcgtccgcgc cggttgcaaa
      181 gagaatcttg ccgtcgaaca cggaaggatc caccaccgcc gtcggcaggc cgtagccttc
      241 gccgtactgc acagtcttga ccgtattgga taaaatcggg ttcttcggcg gcgcggtctc
      301 cacagcaacg ctttccgtcg gctcgttaga aaacagcggc tcgccgctct gcacgcgcgc
      361 ggtgatgaac ggcacgatga catagatcgt cagcagcgca aaagccgcca gcaggacgag
      421 cgccagcacg agcttcagcg cggggccgaa cgacgttgcg tcgaggttcc acctgcgccg
      481 ggacggcgta tatttgagat gtttgcttga acgtttcacg gttaccattt tacagcaacc
      541 attcggttcg gtcaacggaa gaaagcgctg aagaattttg ctcgaaagct cccgctgttc
      601 agcgggagct ttcgcagccg tgtccggcca tccattccat ctggaatgcc aaaacgcatt
      661 ggttatcagg gtttgttcgc agccgagggg attggaaacg aaaccgcgta ctgcggatag
      721 taactggtgc ttgtgtcgaa tccaccgtaa tactcgttcc agagtgagaa aggctcgtcc
      781 agattcagcg gcgtagtctt gccgcgaacc accagctcgc cggcttggtc ggggctgagt
      841 tcgtagtaag ccgtgcagta actaatatat ggaatcagtg caagctcgtc gatctccggc
      901 aggaggccga gcggaacgcc gtacgcttgc ctgcaatgcc agacgcgctt agagggttcc
      961 gccgcgtcaa gcgagcgcat gacgccgctg acagtccatt cacccgtttc gccgttgatg
     1021 agcagctgaa actgcattcc gtaggtacct gaaaacgcct ggatctcctc ctccgtccag
     1081 ccggaaggaa acgtaatctc gacctgcaga ttctttattc ccgttgcgtc gacttctacg
     1141 cttagggctt tcatcttcaa cccgtccagc gaaagtacgc ggttcgtata tacgttgtag
     1201 ccgtacgtgc ttttgtccac gccgctgtcg tcaaagtacg tcagtatggt ttcaccttcc
     1261 cattccgccg aatcggggtt tcctgccgtt tgttttacaa acgttcggta tgtcacggcg
     1321 tccacgggga tactgccgat atcggcttcg agcacggtcg tggggacagc cgtcgcggag
     1381 ccttccagct cgatttgcaa taagagcgaa agcttcccct cgatgaggcc ggatgcgtcc
     1441 gcaagtgcct tcaaacgttc aggatcgact ttcatagcgg cataggcttt tacgtcgtgt
     1501 tcggcaaggt atgccgctgc caaagcgtcc gcgtcctgcg gattttcgga agcctgcctg
     1561 aacagcacgg caatcgtttc gtcctcaaca ac
//
LOCUS       DAOMGY010000455         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_92674_length_1586_cov_3.370999, whole genome shotgun sequence.
ACCESSION   DAOMGY010000455 DAOMGY010000000
VERSION     DAOMGY010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92674_length_1586_cov_3.370999"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PLR57_07160"
     CDS             complement(<1..1378)
                     /locus_tag="PLR57_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41271.1"
                     /translation="MKRICILSVILSALSIGSLSAQNLAISIGIRETGGSGPAFANGG
                     ETIKITVTPAAGYKLKPNSLTVKTVEGSVEATVPVNVDANGEYTYKLPVPVGGDTNPM
                     NVVITGTFITDPSYTGTTSTNQQKKSFSLGIGIAVAVTLHSNYAYIKNGTISAGSLEL
                     TATSGSESSQLVFAADSKAGYAQGDFGLAGAVTVHVASAKTKAIVGDKPSITLTEGGT
                     LKIASASYETVTTNAVATSSGTASRLGVGAGISVGVFGADVFAKVADGASIKVLNSGS
                     IKSAEITANHASTETLKSQAGSQGGISITPVLSLLISGARTEATLGTSNQLLSASEDI
                     TLTAKNSTTRELGANAAAAGGKVGIGASFTISVLNDSTTAAVQRSARARNVRVSATGH
                     SSLKSTSRAGSQGASSQNSSSGTTTTSSTTDGTADSSSGEEGGESDKQADKSLGGGAK
                     LAGASGSSN"
BASE COUNT          281 a          462 c          483 g          360 t
ORIGIN      
        1 cgttggaact gccgcttgcc ccggcgagct ttgcgcctcc gccgagactc ttatccgctt
       61 gtttgtctga ttccccgccc tcttcgccgg aactgctgtc cgccgttcca tcggtcgtgg
      121 aagaagtagt tgttgtcccg ctggaagagt tctggctgga agcgccctgc gaacccgcgc
      181 gggaagtcga tttgagtgaa ctgtgcccgg tcgcgctgac ccgcacgttg cgggcgcgcg
      241 cgctgcgctg aactgcggcg gtcgtactgt cgttgagaac ggaaatcgta aacgacgcgc
      301 caatgccgac ctttccgccg gctgcggcgg cgtttgcgcc cagttcacgg gttgtgctgt
      361 ttttcgcggt gagcgtaata tcttcgctgg cggagagcag ctggttcgaa gtgccgagcg
      421 ttgcttccgt ccgcgcgccg gagattagaa gcgaaagaac aggcgtaatg gaaataccgc
      481 cctgcgaacc tgcctgcgat ttgagcgttt ccgttgaagc gtggtttgcc gtaatctccg
      541 cgctcttgat cgaaccgctg ttgagcacct tgatggaagc gccgtcggct accttggcaa
      601 acacatccgc gccgaacacg ccaaccgaaa tgcccgcgcc gacgcccagg cgggaagccg
      661 tgccgctgct cgttgcaacc gcgttggtgg tcaccgtttc gtaggaggcg cttgctatct
      721 tcagcgtgcc gccctcggta agcgtaatgg aaggtttgtc gccgacgatc gctttggtct
      781 ttgcgctggc aacatgcacg gtcaccgcgc ccgcgaggcc gaagtcgccc tgcgcgtatc
      841 ccgctttgga gtccgccgca aacacaagct gactgctctc gctgccggac gtcgcggtga
      901 gctcaaggct gcccgcagaa atcgtgccgt tcttgatata agcgtagttg gaatgcagcg
      961 tcaccgcgac cgcgatgccg atgccgagcg agaaggattt tttctgctgg ttggtcgaag
     1021 tggttccagt gtagctcggg tcggtgatga acgtccccgt aatgacgacg ttcatggggt
     1081 tcgtgtcccc gccgaccgga acgggcagct tgtaggtgta ttcgccgttt gcgtcaacgt
     1141 tcaccggaac ggttgcttcc acggaacctt ccaccgtctt gaccgtcaag gagttcggct
     1201 tgagcttgta acccgcagcg ggcgttacgg taatcttgat cgtttcgccg ccgttggcaa
     1261 aggcgggtcc agagccgcct gtttcccgga tgccgattga gatggccaga ttctgtgcgg
     1321 acaacgaacc gatgctcaaa gcgctgagaa taacgctaag gatacagatt ctcttcatgg
     1381 gatgtttgct cctggctgat tgcatgacac agctcggact gcgacatggc agggcttttg
     1441 gttgagcttg cttttcacag tcaggctccg gctcaacgat cctacgtacc gcttccttag
     1501 atccgcatcc gatggctgtc aacttgaaat cgtgcgcctg gcactctcaa cagcgccaat
     1561 tcataaagac gaaaaggctg gagggg
//
LOCUS       DAOMGY010000456         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_92686_length_1586_cov_3.035271, whole genome shotgun sequence.
ACCESSION   DAOMGY010000456 DAOMGY010000000
VERSION     DAOMGY010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92686_length_1586_cov_3.035271"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..953
                     /locus_tag="PLR57_07165"
     CDS             123..953
                     /locus_tag="PLR57_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016819255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQQ41272.1"
                     /translation="MNYQIVADSCCDLTPELKKEMDVISVPLTMTLGSVSYTDDESLD
                     MPAFMAAMKACKERIGSAAPSPGMYYDAFRNSEESFAVTLSANLSASYSSAMLGKSLA
                     AEEGKNVHVFDSKSASAGEVLIVMKLHQLIRSGLSKLEIIERIERFIKGMKTYFVLDN
                     VDTLRKNGRLSRIAGTIVSVLHLRPIMGADSEGHISLYSNAQGWKQALKKLADTIEAS
                     GKPTEGESMVITHCNNPSMAETLKSAIEARYHFSEILVLPTRGLSSIYANEMGVIIAF
                     "
     gene            1005..>1586
                     /locus_tag="PLR57_07170"
     CDS             1005..>1586
                     /locus_tag="PLR57_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQQ41273.1"
                     /translation="MNPYVLTCCSTADLPHSYFEQRKIPYVCFHFLFGEEVYPDDLGQ
                     SMPFDTFYSRIAAGAMPTTSLVNVDEFITFFEPFLKEGKDILHISFSSGLSGTYGSAR
                     LAAEDLLARYPERTIKLVDSLGASSGYGLLVDAVADKRDGGATLEEAYSFAEKTKLHV
                     HHWFFSGDLSHYKRGGRISAASFFVGSMLSIIPL"
BASE COUNT          345 a          459 c          418 g          364 t
ORIGIN      
        1 ggttggcgtg aacaatttcc aatagaatgg taacaggttt ttccgttgct gcggaggact
       61 ttctcaacgt gcaggcggta cagccgtctg tcaagactac caaagcaaag gagaatcctc
      121 aaatgaacta tcaaatcgtt gccgacagct gctgcgatct tacgccggaa ctcaaaaaag
      181 aaatggatgt catctccgtc ccgctgacaa tgacgctggg ctccgtttcc tatacggatg
      241 acgaatccct cgacatgccg gcctttatgg ccgcgatgaa agcctgcaag gagcgcatcg
      301 gttcggcggc gccttcgccg ggcatgtatt acgacgcgtt ccgcaattcg gaggaatcgt
      361 tcgcggtcac gctgtccgcc aacctgtccg cctcgtattc cagcgcaatg ctcggcaagt
      421 cccttgccgc agaagaaggc aaaaacgtcc atgtgttcga ctccaagagc gcttcggcgg
      481 gcgaagtgct gattgttatg aaactgcacc agctgattcg cagcggtctt tcgaaacttg
      541 aaattatcga gcgcatcgag cgttttatca agggaatgaa aacctacttc gttttggaca
      601 acgtcgatac cctgcgcaag aacggacgcc tgagccggat tgccggtacc atcgtttccg
      661 tactgcacct gcggccgatt atgggcgcgg attccgaggg gcatatttcg ctctattcca
      721 acgcgcaagg ctggaagcag gcgctcaaga agctggccga taccattgaa gccagcggca
      781 agcccaccga aggcgagagc atggtcatta cgcactgcaa caacccctcg atggccgaaa
      841 cgctgaaaag cgccattgaa gcgcggtatc atttttccga aattctggtg ctgccaacgc
      901 gcggcctcag ctccatctat gccaacgaaa tgggcgtcat tatcgcgttt tagcgaaaaa
      961 ttccgtccat tcacacgaaa attttatacc ggaggaaatg ccctatgaat ccttacgttc
     1021 ttacctgctg ctcaaccgca gatctgccgc attcgtattt tgagcagcgc aagattcctt
     1081 acgtctgctt ccattttctc tttggcgagg aggtctatcc ggacgacctc ggtcagagca
     1141 tgccgtttga cacgttctac tcgcgcattg cggcaggtgc catgcccacg acatcgctgg
     1201 tgaacgtgga cgagttcatc acgtttttcg agccgttcct caaggaaggc aaggatattc
     1261 tgcatatttc gttctcgtcg ggactctccg gcacctacgg ctcggcaagg ctcgccgccg
     1321 aagacctgct ggccaggtac ccggagcgca cgatcaagct cgtcgattcc ctcggcgctt
     1381 cctccggcta cggcctgctc gtggacgccg ttgccgacaa gcgggacggc ggcgctacgc
     1441 tggaagaagc gtactccttt gctgaaaaga ccaagctgca tgtgcatcac tggttcttct
     1501 ccggcgatct ttcgcactac aagcgcggtg ggcgcatttc cgccgcttcg ttctttgtcg
     1561 gctctatgct gagcatcatc ccgctg
//
LOCUS       DAOMGY010000457         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_92916_length_1584_cov_2.081753, whole genome shotgun sequence.
ACCESSION   DAOMGY010000457 DAOMGY010000000
VERSION     DAOMGY010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92916_length_1584_cov_2.081753"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLR57_07175"
     CDS             complement(<1..375)
                     /locus_tag="PLR57_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HQQ41274.1"
                     /translation="MKVLLKNCRVYDGTGTDAFNGDILIQDDRILDVWSQIKAEDAEV
                     IDLGGKSVSPGFIDAHSHNDWFAAKREANRYFEPFVRQGITSFITGNCGLSMTGFAPD
                     SLNKDKIGAGLFHMTDLTGEYAS"
     gene            622..1008
                     /locus_tag="PLR57_07180"
     CDS             622..1008
                     /locus_tag="PLR57_07180"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HQQ41275.1"
                     /translation="MKETLSRHEWVIMETLWQRAPLFLSDLMSAMETSVDWNRSSYLT
                     YLKRMTDKGYIGYETVRGSRRYIPLIRREDCVAAESAAMLEKMTGQSAKLFLVSMVQK
                     SGLSESDRAELQALISRLGEEQKEGE"
     gene            1009..>1584
                     /locus_tag="PLR57_07185"
     CDS             1009..>1584
                     /locus_tag="PLR57_07185"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HQQ41276.1"
                     /translation="MNILRTLLEISIYAAVIFLAILLFRRLFHKSLSPLVKYALWFLL
                     LVRLLLPVTLDAGVRLFTASSENELYAAAVPQEQATEEPAAQAGADAASQETVAPALT
                     AGTNTANKTLSTQQTLLMVWLSGVVLVGGWMSVSYAALRRKMRKNAAEPSERMLALLA
                     EAKAETGVRGNIRICCQYAVGAPAMVFPNLLF"
BASE COUNT          365 a          422 c          466 g          331 t
ORIGIN      
        1 ggaggcatac tcgcctgtga gatcggtcat atggaacaga cccgcgccga tcttgtcttt
       61 atttaagcta tccggcgcaa aaccggtcat ggagagcccg cagttgccgg tgataaacga
      121 ggtgatgccc tgacgcacga acggttcgaa gtagcggttc gcttcgcgct ttgccgcaaa
      181 ccagtcgtta tgcgaatgtg cgtcgataaa gccgggggaa acggatttcc cgccaagatc
      241 gatcacttcc gcgtcttccg ccttgatctg cgaccaaacg tcgagaatgc ggtcgtcctg
      301 tatcaaaatg tcgccgttga acgcatccgt tcccgtgccg tcgtagacgc ggcagttttt
      361 cagtaaaacc ttcataaatc taacctctca tcccaatact tcagcatacc atacaacgcg
      421 aaaaatgaga agtttcgcgt atcgtaaacc gcgattgcgc ctgcgcattt ctgccgccgg
      481 agaactgctt tccactgcgc ggaaaaagaa tattgacaag gctggatatc atgtctacaa
      541 tagtagatac tgaaactgca gcagtagaca aagatgcaag cccgcgtctc ttgacgacgg
      601 acggcaaaag gagtgtacga gatgaaagaa acgctttcaa ggcacgaatg ggtgattatg
      661 gaaacgctct ggcagcgcgc gccgctgttt ttatccgacc tgatgagcgc aatggagacg
      721 agcgtggact ggaaccgttc gagctattta acctatctca agcgcatgac ggacaaaggc
      781 tacatcggct acgaaaccgt gcgcggcagc cgaaggtata tcccgctgat ccggcgtgag
      841 gactgcgtgg cggcggagag cgcggccatg ctggaaaaga tgaccggaca gagcgcgaag
      901 ctctttttag tgagcatggt gcaaaaaagc gggctgagcg agagcgatcg cgcggaactg
      961 caggcgctga tttcgcgtct gggcgaagag caaaaggagg gcgaatagat gaatatcctc
     1021 cgaacgctac tggaaatctc gatttacgcc gcggtgatct ttctggcaat cctgctgttt
     1081 cgcaggttgt tccataaaag cctttcgcct ctggtgaaat acgcgctttg gtttctgctg
     1141 cttgtgcgac tgctcctgcc ggtgacgctg gacgcgggcg tgcggctgtt taccgcctct
     1201 tcggaaaacg aactgtatgc cgcggctgtg ccgcaagaac aggccacgga agaacccgca
     1261 gcgcaggccg gtgcggatgc tgcttcgcag gaaacggttg cacccgcact gactgccggg
     1321 acaaacacgg caaacaagac gttgtccacg cagcagaccc tgcttatggt ctggctttcc
     1381 ggcgtcgtgc tggttggcgg ctggatgagc gtttcctacg ccgcgctgcg ccggaagatg
     1441 cgcaaaaacg cggcggagcc gagcgaacgt atgctggctc tgctggcgga agccaaggcg
     1501 gagacgggcg tgcgcggaaa catccgcatt tgctgtcaat acgccgtcgg tgcacctgct
     1561 atggtgtttc ccaacctgct gttt
//
LOCUS       DAOMGY010000458         1583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_92975_length_1583_cov_2.652487, whole genome shotgun sequence.
ACCESSION   DAOMGY010000458 DAOMGY010000000
VERSION     DAOMGY010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92975_length_1583_cov_2.652487"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLR57_07190"
     CDS             <1..338
                     /locus_tag="PLR57_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HQQ41277.1"
                     /translation="AATYNALVAPCADLYSGLNNGWFIFPILAGGTTYLSTWIMQRQQ
                     PQSAATGSTNKLMQWLMPVMSFVFCLQYNASFAIYWTFSNIFSLGTSLLIDRSFAKQK
                     AALEEAQNS"
     gene            335..1198
                     /gene="jag"
                     /locus_tag="PLR57_07195"
     CDS             335..1198
                     /gene="jag"
                     /locus_tag="PLR57_07195"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HQQ41278.1"
                     /translation="MKSMEFSGHSVDEAIFVGLTEMGVTIDEVEIETIQSETKGLFGI
                     GAKMAVVRLTEREVPLVMEMEQVYRKETESASRRDAQPRRDSRPPRGDRNDRYDHGSR
                     PQREGGYSQQARDSRPRDHAPEPVVEEHYDYSEELAKNTPCAVFLGELLAKMNVPAPV
                     LAAETENGLRLCINAETMGLLIGRRGETLDAMQYLVSLVANKDRKDDGYLRVTLDTEG
                     YRSRREETLKRLARKNAVHVRQTGRPVSMEPMNPYERRILHSALQGFTGVTTHSEGEE
                     PNRHVVITPSK"
     gene            1287..>1583
                     /locus_tag="PLR57_07200"
     CDS             1287..>1583
                     /locus_tag="PLR57_07200"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQQ41279.1"
                     /translation="MEDTIYAPSSAIGGAIAVIRVSGDQARRVSELLNRDIARQPRKL
                     TYVKLQQNGELIDECMAAFLPAPGTYTGEDMAELNCHGGMQTIQRALAALGSLGF"
BASE COUNT          340 a          470 c          483 g          290 t
ORIGIN      
        1 tcgccgcaac ctataacgcg ctggttgcgc cctgtgcgga tctctacagc gggctgaaca
       61 acggctggtt catcttcccg attctcgcgg gcggaacgac gtatctttcc acgtggatca
      121 tgcagcgtca gcagccgcag agcgcggcga ccggcagcac caacaagctc atgcagtggc
      181 tcatgccggt gatgtcgttc gtgttctgcc tgcagtacaa cgcgagcttc gctatttact
      241 ggacgttcag caacatcttc tcgctgggca cctccttgtt gatcgatcgt tcgtttgcca
      301 agcagaaggc ggcgcttgag gaggcgcaga actcatgaag agcatggagt tttcggggca
      361 cagcgtagac gaagccattt tcgtcggtct caccgagatg ggcgtgacca ttgacgaagt
      421 ggaaatcgaa acgatacagt ccgaaaccaa aggcttgttc ggcattggcg caaagatggc
      481 tgttgtgcgg ctcaccgagc gcgaggttcc actggttatg gagatggagc aggtctaccg
      541 caaggaaacc gaatccgctt cccgccggga cgcgcagccg cgacgcgaca gccgcccgcc
      601 gcgcggcgac aggaatgacc gttacgacca cgggtcaaga ccgcagcgcg aaggcggata
      661 ttcgcagcag gcaagggatt cgcgtccgcg cgaccatgcc cccgaacctg ttgtggaaga
      721 acactatgat tacagcgagg aactcgcaaa gaatacccct tgcgcggtgt tccttggcga
      781 actgcttgca aagatgaatg ttcccgcgcc ggttctggcg gcggaaacgg aaaacggcct
      841 tcgcctctgc atcaatgccg agactatggg acttttgatc ggccgacgcg gcgaaacgct
      901 ggacgctatg cagtatcttg tttcgctggt tgccaacaaa gaccgcaagg acgacgggta
      961 cctgcgcgtt actctcgata ccgaagggta tcgctcccgc cgggaagaga cgctcaagcg
     1021 ccttgcgcgt aagaacgcgg tgcacgtccg ccagacgggg cggccggtct cgatggaacc
     1081 catgaacccg tacgagcggc gcatcctgca cagcgcgctg cagggattca ccggcgtgac
     1141 cacccacagc gagggcgaag aacccaaccg ccacgtggtc attacgccat caaagtaaca
     1201 tcttcacgag ccgccgcctt tgagcggcgg ctttctctat tttggcgtac aagccgccga
     1261 acggatttgg aataaggaga acctgcatgg aagacacgat ttacgcgccg agttccgcca
     1321 tcggcggcgc gattgcggtg atccgcgttt ccggcgatca ggcgcggcgc gtcagcgaac
     1381 tgctgaaccg cgacatcgcg cggcagccgc gaaaactgac ttacgtaaag ctccagcaga
     1441 acggcgagct gatcgacgaa tgcatggcgg cttttttgcc cgcgcccgga acgtataccg
     1501 gcgaggacat ggcggaactc aactgccacg gcggcatgca gaccattcag cgcgcattag
     1561 ccgcgctggg ttcgctcggg ttt
//
LOCUS       DAOMGY010000459         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_93471_length_1577_cov_3.902760, whole genome shotgun sequence.
ACCESSION   DAOMGY010000459 DAOMGY010000000
VERSION     DAOMGY010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93471_length_1577_cov_3.902760"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..95
                     /locus_tag="PLR57_07205"
     CDS             <1..95
                     /locus_tag="PLR57_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002880277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQQ41280.1"
                     /translation="TDLYEDTNGSGNLHEYNIPTFVVTRAVKPR"
     gene            complement(201..1097)
                     /locus_tag="PLR57_07210"
     CDS             complement(201..1097)
                     /locus_tag="PLR57_07210"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQQ41281.1"
                     /translation="MYYLLSLLTGILVAIMITVNGGLTTLYGVYSATVIIHIVGLLLI
                     GGVILLRREKPFRKGLPWYLYIGGVIGVASTVFTNYAFGKISVSAILALSLFGSSVMG
                     LLVDQFGLFKMRVHRFRPHQLPGLMLVLGGIVCLMIGSFSLLPVLAVFASGILLVVSR
                     SFNSRLADQTSMYSSTFFNYVCGLCTAIPVFLLLGRGEPVVAKFAFSPDWWIYLGGAI
                     GVVTVFLTNVVVVKIPALYISLLMFVGQVFTGVLLDALLDGAFSLQNLIGGVFVALGM
                     TLNLLLERRESRKTAALYREQE"
     gene            1218..>1577
                     /locus_tag="PLR57_07215"
     CDS             1218..>1577
                     /locus_tag="PLR57_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQQ41282.1"
                     /translation="MKKSLLPPGYADKLTQYDLGALDTSGAVLLKYDSGEWLLNEGHP
                     IEYLYILLSGKAKVSVSVENGRSLLLCYYISEGMIGDMELMTGKHEAFSSMQAVTPIT
                     LIGLPLERYASVLRANLP"
BASE COUNT          398 a          453 c          429 g          297 t
ORIGIN      
        1 tgaccgacct gtatgaggat accaacggct ccggcaacct gcacgagtat aatataccaa
       61 cgttcgtcgt cacccgcgcg gtgaaaccga gatagttttt cggggactcc gtccccaagt
      121 ccctggccaa aggggcagac gcccctttgg aaaccccaca aaaaaagccg catctgattt
      181 cagatgcggc tttgtgtttt ctattcctgc tctcggtaga gagcggcggt tttgcgtgat
      241 tcccttcgtt ccagcagcag gtttaacgtc atccccaatg ctacgaatac gccgccaatc
      301 agattttgca gcgagaatgc tccgtcgagc agcgcgtcca gcagaacgcc cgtgaacacc
      361 tggcctacga acattagaag cgaaatatac agcgcgggaa ttttgaccac cacgacgttg
      421 gtgagaaaca ccgtaacaac tccgatcgcg ccgccgaggt agatccacca gtcgggcgaa
      481 aacgcaaact tcgccacaac cggctcgccg cgacccaata gaagaaacac ggggatggcg
      541 gtgcaaagcc cgcagacgta gttgaagaac gtgctcgagt acatgctggt ctgatccgcc
      601 agacgcgaat tgaacgaacg ggaaacgacc agcagaatgc cgcttgcaaa caccgcgagc
      661 accggaagaa gcgagaacga gccgatcatc aggcagacaa tgccgccgag cacgagcatc
      721 agccccggca gctggtgcgg gcgaaagcgg tgcacgcgca ttttgaacag cccgaactgg
      781 tcgaccagca aacccatcac gctcgagccg aacagactca gcgctagaat ggccgaaacg
      841 ctgattttgc cgaacgcata gtttgtaaac accgtggaag ccacaccgat gacgccgccg
      901 atgtagagat accacggtaa tcccttgcga aacggctttt ccctgcgcag caaaatcacg
      961 ccgccgatga gcagaagccc cacaatatgg atgatcaccg ttgcggaata cacgccgtag
     1021 agcgtggtca gcccgccgtt gacggtaatc atgattgcca cgaggatgcc cgtgagcagt
     1081 gaaagcaaat agtacatgca ggtctctttt ctgcgctttt cgtgcggtat tacgaacaag
     1141 agtataaccc gctcacttga cgcggggtat gacatgtgtc acactcaaaa caggacgatt
     1201 aaagggggga tttgacaatg aagaagtctc tgctgccgcc cggctatgcc gataaactga
     1261 cgcagtatga ccttggcgcg cttgatacga gcggcgcggt gctgctcaaa tacgacagcg
     1321 gcgaatggct gctcaacgaa ggccacccta tcgagtatct ctatatcctg ctctccggca
     1381 aggcgaaggt ttctgtcagt gttgaaaacg ggcgcagtct gctgctttgc tactatatat
     1441 cggaaggcat gatcggggat atggagctca tgaccggcaa gcacgaggcg ttttcctcca
     1501 tgcaggcggt cacgccgatt acgctcatcg gccttccgct ggagcggtac gcttccgtac
     1561 tccgcgcgaa cctgccg
//
LOCUS       DAOMGY010000460         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_93496_length_1577_cov_2.741787, whole genome shotgun sequence.
ACCESSION   DAOMGY010000460 DAOMGY010000000
VERSION     DAOMGY010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93496_length_1577_cov_2.741787"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLR57_07220"
     CDS             <1..502
                     /locus_tag="PLR57_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signaling recognition particle receptor family
                     protein"
                     /protein_id="HQQ41283.1"
                     /translation="RAAAAEQLGEWAKRADVPMVKHNEGADPAAVVFDAIASYKAKGC
                     DLLLCDTAGRLHNKTNLMNELGKIKRVITRELPETPCETMLVLDAVTGQNAVAQAKAF
                     AEVCQLDGVILTKLDGTAKGGVVISICETLHVPVRFVGVGEGIDDLQPFDAAFFAEAL
                     LSQEDE"
     gene            615..947
                     /locus_tag="PLR57_07225"
     CDS             615..947
                     /locus_tag="PLR57_07225"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HQQ41284.1"
                     /translation="MNRLVELGQLVDWYGAFLTQRQRSLVRQYAFEDCSLGEIAEREG
                     ISRQAVRDAITTAEAELRGMEQKLGLIEKNERTRAILSELMATPLDEHQRELAEKLLE
                     LSSGEEEA"
     gene            989..>1577
                     /locus_tag="PLR57_07230"
     CDS             989..>1577
                     /locus_tag="PLR57_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HQQ41285.1"
                     /translation="MAFEGVSSKLQAIFKRLNGRGKLTEADVNEAMREIKLALLEADV
                     NFLVVKDFIKIVSARAVGSEVLESLTPGQQVIKIVNEELTRLMGGENAKLDFGTQKPA
                     VILMAGLQGAGKTTMCGKLGAYIKKQYGKQPLLAACDIYRPAAIQQLQIVGEKLDLPV
                     YEHGTQNPVLTAKEAVAEAKRRFLDVVIIDTAGRLH"
BASE COUNT          375 a          435 c          476 g          291 t
ORIGIN      
        1 ccgtgcggcg gcggcggagc aactgggcga atgggcaaag cgcgcggacg tacccatggt
       61 taagcacaac gaaggagcgg atcccgcggc ggtggtgttt gacgcaatcg cttcctacaa
      121 ggccaaaggc tgcgacctgc tgctctgcga cacggcgggg cggctgcaca ataaaaccaa
      181 cctcatgaac gaactcggca agatcaagcg cgtcatcacg cgcgaactgc cggaaacacc
      241 ctgcgaaacc atgctcgtgc tggacgcggt gacggggcag aacgccgtgg cgcaggcaaa
      301 agcatttgcc gaagtctgtc agctcgacgg cgttatcctc accaagctcg acggcacggc
      361 caaaggcggc gtggtgattt ccatctgcga aacgctgcat gtgccggtgc gctttgttgg
      421 cgttggcgaa ggcattgacg acctccagcc gtttgacgca gcgttctttg ccgaagcgct
      481 tctttcgcag gaagacgagt aaaaaccaaa ataatccgct ttttaagggc atttccgaac
      541 tttttcttga caaaacggcg ttcctcctta taatggacag agtgtaaagc tatttcactt
      601 tacaggaatg ccgaatgaat cgattagtgg aactgggaca attagtggac tggtacggcg
      661 cttttctgac acagcgccag cggtcgctcg tgcgccagta cgcgtttgag gactgctcgc
      721 tgggcgaaat tgccgagcgc gagggcatct cgcgtcaggc cgtgcgggac gcaatcacca
      781 cggcggaagc ggaactaagg ggcatggaac aaaagcttgg cctcattgaa aagaacgaac
      841 gcacccgcgc gattttatcc gaactgatgg caactccgct cgacgagcat cagcgggaac
      901 tggcggagaa actattggag ctttcctccg gcgaggaaga agcgtaaccc cgtttttatg
      961 aatgacagat aatatttgga gatagatcat ggcgtttgaa ggagtctcgt ccaaactgca
     1021 agccattttc aagcggctga acggacgcgg aaagcttacc gaggcggacg taaacgaagc
     1081 catgcgcgag attaaactcg cgctgttgga agcggacgtc aacttcctcg tggtcaagga
     1141 cttcatcaag atcgtttccg cgcgcgccgt cggcagcgag gtactcgaga gtttaacccc
     1201 cggccagcag gtgattaaga tcgtcaacga ggagctcacg cgcctcatgg gcggcgaaaa
     1261 cgccaagctg gactttggca cgcagaaacc cgcggtcatc ctgatggcgg gcctgcaggg
     1321 cgcgggcaag acgaccatgt gtggcaagct cggcgcgtat atcaaaaagc agtatggcaa
     1381 gcagccgctg ctcgccgcct gcgacatcta tcgcccggcg gccattcagc agctccagat
     1441 cgtcggtgaa aagctcgacc tgccggtcta cgaacacggc acgcagaacc ccgttctcac
     1501 cgctaaagaa gcggtggcgg aggccaaacg gcggtttttg gacgtggtca tcatcgacac
     1561 cgcgggccgg ctgcaca
//
LOCUS       DAOMGY010000461         1576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_93601_length_1576_cov_2.809993, whole genome shotgun sequence.
ACCESSION   DAOMGY010000461 DAOMGY010000000
VERSION     DAOMGY010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93601_length_1576_cov_2.809993"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1576
                     /locus_tag="PLR57_07235"
     CDS             <1..>1576
                     /locus_tag="PLR57_07235"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQQ41286.1"
                     /translation="HEKLSPVLAMYRANSFDEALDKAERLIADGGYGHTSSVYLNAVT
                     EQAKLDKFSARMKTCRILVNTPSSHGGIGDLYNFKLAPSLTLGCGSWGGNSTSENVGV
                     KHLLNIKTVAERRENMLWFRAPEKVYFKKGSLPVALSELKDVLGKKRAFIVTDTFLYE
                     NGYTKPITNKLDEMGIAHESFFNVAPDPTLACAREGAKQMAAFKPDVIIALGGGSAMD
                     AAKIMWVLYEHPEADFQDMAMRFADIRKRVYTFPKMGEKAYFIAIPTSAGTGSEVTPF
                     AVITDETTGVKYPLADYELMPDMAIVDADMMMNAPKGLTAASGIDAVTHDLEAYASML
                     ATDYTDGPALRSLKMIFEYLPRAYDNGQNDPVAREKMGNAATMAGMAFANAFLGVCHS
                     MAHKLGAFHHLPHGIANALMIDEVLRFNAVEVPVKMGTFSQYDHPHTLARYAEVADYL
                     GLKGKTDADKLEALIAALDELKAKVGIKKTIRDYGIDEADFLYRLDAMTEQAFDDQCT
                     GANPRYPLMSEIKQMYL"
BASE COUNT          362 a          497 c          435 g          282 t
ORIGIN      
        1 gcatgaaaag ctttcgcccg tgctggccat gtaccgcgcg aatagctttg acgaagcgct
       61 tgataaagcc gaacggttaa tcgcggacgg cggctacggc cacacctcca gcgtctacct
      121 caacgccgtc accgaacagg ccaaactcga taaattttcc gcgcggatga agacctgccg
      181 catcctcgta aacactccgt cctcgcatgg cggcatcggc gacctgtata acttcaagct
      241 cgcgccctcc ctcaccctcg gctgcggaag ctggggcggt aactccactt cggagaacgt
      301 cggcgtcaag cacctgttaa acattaaaac cgttgccgag aggagagaga atatgctttg
      361 gttccgcgca cccgaaaagg tatacttcaa gaaaggcagc ctgccggtcg cgctgagcga
      421 gctaaaggac gttctcggca aaaagcgcgc ctttatcgtg accgatacgt tcctctacga
      481 aaacggctac acgaaaccca taactaataa gctcgacgag atgggaattg cccacgaatc
      541 gttcttcaac gtcgctcccg accctacgct ggcctgcgca cgcgaaggcg caaaacagat
      601 ggcggcattc aagccggacg tgattatcgc cctcggcggc ggctccgcga tggatgcagc
      661 caagatcatg tgggtgctct acgagcatcc ggaagcggac tttcaggata tggccatgcg
      721 ctttgccgat atccgcaagc gcgtttacac cttccccaag atgggcgaaa aggcgtactt
      781 tatcgccatc cccacttcgg cgggcacggg cagcgaagtc accccgtttg cggtcattac
      841 ggatgaaacc acgggcgtga agtatccgct ggcggactac gagctcatgc cggacatggc
      901 gattgtggat gcggacatga tgatgaacgc ccccaagggt ctcaccgccg caagcggcat
      961 cgacgcggtc acccacgacc tggaagctta cgcttccatg ctggcgaccg actatacgga
     1021 cggccctgcg ctgcgttcgc tcaagatgat cttcgagtac ctgccccgcg cctacgacaa
     1081 cggccagaac gaccccgtcg cccgcgaaaa gatgggcaac gccgccacca tggcgggcat
     1141 ggcgttcgcc aacgcgttcc tcggcgtgtg ccacagcatg gcgcataagc tcggcgcgtt
     1201 ccatcacctg ccgcacggca ttgccaacgc gctgatgatt gacgaggttc tccgcttcaa
     1261 cgccgtggag gttcccgtta agatgggcac attctcgcag tacgaccacc cgcataccct
     1321 cgccagatac gcggaagttg cggattacct cggcctcaaa ggcaagaccg acgcggacaa
     1381 gctcgaagcg ctgattgcgg cgctggacga gctcaaggcg aaagtcggca ttaaaaagac
     1441 gatccgcgat tacggcattg acgaagcgga ctttctatac cgcctggacg cgatgaccga
     1501 gcaagccttc gacgaccagt gcaccggcgc caacccgcgc tatccgctca tgagcgaaat
     1561 caagcagatg tatctc
//
LOCUS       DAOMGY010000462         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_93799_length_1574_cov_2.282423, whole genome shotgun sequence.
ACCESSION   DAOMGY010000462 DAOMGY010000000
VERSION     DAOMGY010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93799_length_1574_cov_2.282423"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PLR57_07240"
     CDS             complement(<1..746)
                     /locus_tag="PLR57_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41287.1"
                     /translation="MKQGKRTFFELLVILSGLVMALVVILLCSDEPATALKFFFTGPF
                     SSAYFFGDMLNTAVPLIICGLAASVSFTASVWNLGLEGMVYFGMLTGTLSAVAMEGWP
                     AAVAIPLMFIAAFAGGALLAAICNFLKRKFDLDVMMSSLMLANVVFYLIFMLVEGPLR
                     DMESGQGVTTKAIPEAFRFGRILEPSDLNTTLFLALALAVIVYFALKRSRVGYEIRMT
                     GQSPAFARYGGVSTAAVASLAMIFSGGLSG"
     gene            complement(743..>1574)
                     /locus_tag="PLR57_07245"
     CDS             complement(743..>1574)
                     /locus_tag="PLR57_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ41288.1"
                     /translation="SLSPELGRTTCCPGGSVLDVTNLFVKNGPLFMVKNASFRVCAGE
                     IVGIAGVNGNGQQELLEALFGLRDTASGSILLSGADISRFSPSARRKAGMGYIPEDRI
                     HVGSCATASISENLLIDRRSQKPYCRGGWLSPRPLEQLSNDLIARYSIKAQDGRHIVG
                     GLSGGHIQRTILARELTAEPKLLLAAEVTMGLDVLSARYIHDEMLALREKGLGILLVS
                     SNINEILELSDRVLVLRGGEITASLVNNAALTREEIGEYMLGVKRMEPETCTAQEAMA
                     "
BASE COUNT          342 a          462 c          497 g          273 t
ORIGIN      
        1 atgccggaaa gcccgccgga gaagatcatc gcaaggctcg cgaccgccgc cgtgctgacg
       61 ccgccgtagc gcgcgaacgc ggggctctgc ccggtcatgc ggatttcgta gccgacgcgg
      121 gagcgcttaa gcgcaaagta gacgatcacc gcgagcgcca gcgcgagaaa cagggtggtg
      181 ttcaggtcgg aaggctccag aatgcggcca aagcggaacg cctccgggat ggcctttgtg
      241 gtaacgccct gaccggattc catgtcacgc agcggccctt ccaccagcat gaagatgagg
      301 taaaagacca cgttggcgag catgagcgag gacatcatca catccagatc aaacttgcgc
      361 ttcaaaaagt tgcagatagc ggctaaaagt gcgccgcccg cgaatgcggc gatgaacatc
      421 agggggatag caaccgcggc tggccagcct tccattgcca cggcggaaag cgtgccggta
      481 agcataccaa agtataccat accctcgaga ccgaggttcc agacggaagc ggtaaacgaa
      541 acgctcgcag ccagcccgca gatgatcagc ggcacggcgg tgttgagcat gtcgccaaag
      601 aaatacgcgc tcgaaaacgg gcccgtaaag aagaacttga gcgcggtggc gggttcgtcg
      661 ctgcagagca ggatgacgac aagagccatc acaagtccgg agaggatgac gagcagttca
      721 aagaacgtgc gtttgccctg tttcatgcca tcgcctcctg tgccgtgcag gtttcgggtt
      781 ccatccgctt gacgccgagc atgtattcgc caatctcttc acgcgtgagc gcggcgttgt
      841 tgacgaggct tgcggtaatc tcgccgccgc gcaggacaag aacgcggtcg ctgagctcca
      901 gaatttcgtt gatgttgctc gatacgagca ggatgccaag ccctttttca cgaagcgcga
      961 gcatttcgtc gtgaatatac cgcgcggaga gaacgtccag acccatcgtg acttccgccg
     1021 cgagcaagag cttcggctcg gcggtgagtt cgcgcgcgag gatcgtccgc tggatatgcc
     1081 cgccggacag gccgcctacg atatgccgcc cgtcctgcgc cttgatgctg taccgcgcga
     1141 taaggtcgtt cgagagctgt tccagcgggc gcgggctgag ccagccgccg cggcagtagg
     1201 gcttttgact gcgccggtcg attaaaagat tctccgaaat agaagccgtg gcgcaggaac
     1261 cgacatggat gcggtcctcc gggatatacc ccatgccggc cttgcggcgc gcggaggggg
     1321 aaaagcgcga aatatccgcg ccggagagca atattgagcc gctcgcggta tccctcagcc
     1381 cgaacagcgc ttccagcagt tcctgctggc cgttgccgtt gacgcccgca atgcctacga
     1441 tttcccccgc gcagacacgg aagctcgcgt ttttcaccat aaagagaggg ccgtttttga
     1501 caaacaggtt cgttacgtcc aggacgcttc cgccggggca gcaggtggtg cggccgagtt
     1561 cgggagagag cgat
//
LOCUS       DAOMGY010000463         1572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_93957_length_1572_cov_3.385630, whole genome shotgun sequence.
ACCESSION   DAOMGY010000463 DAOMGY010000000
VERSION     DAOMGY010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93957_length_1572_cov_3.385630"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLR57_07250"
     CDS             complement(<1..577)
                     /locus_tag="PLR57_07250"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HQQ41289.1"
                     /translation="MELDALREKIDAVDETMLALFIERMELSGQVAAYKREHALPLLN
                     KTREREILARVTQNAGELEPYVHQFFTNLFELSKARQGELNAGASRIRALIEQALAAP
                     EVVFPHTGVIACQGIEGSNSQAAADKLFPRGNIVYVKSFEAAFNAVESGLCQFAVLPI
                     ENSTNGSVRAVYNLLQKKRFSIVRGTSLCIRH"
     gene            complement(638..>1572)
                     /gene="aroC"
                     /locus_tag="PLR57_07255"
     CDS             complement(638..>1572)
                     /gene="aroC"
                     /locus_tag="PLR57_07255"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQQ41290.1"
                     /translation="SSARQEADEPVFLSGLSNGLTNGAPLCAIIENRDARSGDYESIL
                     DTPRPGHADLAAFLKWNGKAELRGGGHFSGRLTAPLCIAGGIAKQILARRNIFVGAHL
                     KSVAGITDEPFPLYPDRSLFEAIAKKPFPVVSDASGERMQEAILAAGAEGDSVGGVIE
                     CAAVGLPAGLGEPMFGGMENRLSAALFGIPAVKGVEFGAGFGSALLKGSQNNDPYEMA
                     ADGTIAAKTNNAGGILGGITTGMPLVFRAAIKPTPSIAKPQQTVSLSRRENTELEIRG
                     RHDPCVAHRAVPVIEAVCAVVLLDILLEETALWR"
BASE COUNT          288 a          460 c          501 g          323 t
ORIGIN      
        1 cgtggcggat gcagaggctt gtgcctcgca cgatggaaaa gcgctttttc tgcagcaggt
       61 tatacaccgc gcggacggag ccgttggtgc tgttttcaat cgggagcacg gcaaactggc
      121 agagcccgga ttccacggcg ttgaacgcgg cttcaaagct cttgacgtaa acgatgtttc
      181 cgcgcggaaa gagcttgtcg gcggcggcct gcgagttcga cccttcaatg ccctggcagg
      241 cgattacgcc cgtatgtgga aacaccactt ccggcgcggc gagcgcctgc tcgatcagcg
      301 cacggatgcg ggaagccccg gcgttgagct cgccctgacg cgccttggaa agctcgaata
      361 ggttcgtaaa aaactggtgg acatacggct ccagttcgcc ggcgttctgc gttacgcggg
      421 caaggatttc ccgttcgcgg gttttattga gcagcggaag cgcgtgctcg cgtttgtaag
      481 cggcaacctg accgctcagt tccatgcgct ctataaacag cgcgagcatg gtttcgtcta
      541 cagcgtcaat tttttcgcgg agtgcgtcca gttccattga gttgctcctt tcgggggctg
      601 tcaattaata aaaagttgat tttaaagtgg aaacggttca gcgccagagc gcggtttctt
      661 ccagcaaaat atccagcagc acaacggcgc agaccgcctc tatgacgggc accgcgcggt
      721 gcgcaacgca ggggtcgtgc cgcccgcgaa tttcgagctc cgtgttctcc cggcgggaga
      781 ggctgacggt ctgctgcggc ttggcgatgg acggcgtggg cttgatggcc gcgcggaaca
      841 cgagcggcat gccggtggtg atgccgccga ggatgccgcc tgcgttgttg gtctttgccg
      901 ctatggttcc gtccgccgcc atttcatacg ggtcgttgtt ttgagagcct ttgagcagcg
      961 cggaaccgaa ccccgcgccg aactctacgc ccttgacggc gggaatgccg aacagcgcgg
     1021 cggaaagccg gttctccatg ccgccgaaca ttggttcccc aagccccgcg ggcaggccta
     1081 cggctgcgca ctcgatcacc ccgccgacgg agtcgccttc cgcgcccgcc gcaagaatcg
     1141 cctcttgcat gcgttcgccg gaagcgtcgc ttacgacggg aaacggtttt ttcgcgatgg
     1201 cttcaaataa actacggtca gggtaaagcg gaaacggctc gtccgtgatt cccgcgacgc
     1261 ttttcagatg cgcgccgaca aagatgttcc tgcgcgctaa aatctgcttt gcaataccgc
     1321 ccgcaataca gagcggcgcg gtcagcctgc cggaaaagtg cccgccgccg cgcagttccg
     1381 ctttgccgtt ccatttgagg aaagccgcga ggtcggcgtg cccgggacgg ggcgtatcta
     1441 ggatggattc gtagtcgccg gagcgcgcgt cgcggttttc gatgattgcg cagagcggag
     1501 cgccgttggt aagtccgttg gaaagtccgg agagaaaaac gggttcgtcc gcttcctgcc
     1561 gggcgctcga ta
//
LOCUS       DAOMGY010000464         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_94551_length_1566_cov_2.311714, whole genome shotgun sequence.
ACCESSION   DAOMGY010000464 DAOMGY010000000
VERSION     DAOMGY010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94551_length_1566_cov_2.311714"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLR57_07260"
     CDS             <1..693
                     /locus_tag="PLR57_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HQQ41291.1"
                     /translation="MDVKTDRDHPTKRFRPIASGEVPIKTAAILAGILVIASAALHYF
                     AHAVHWYAWIYLLVYLALNMGYSFGLKNQPIIDIAILVSGFLIRVLYGAALSGIEISN
                     WMYLTVIAMSFYLGLGKRRNELIRQPDGAQRKVLRFYNKEFLDKNMYLCLALTIAFYS
                     LWSVDPITTARVQSNGVVWTVPLVIFICMSYSLRIEGNSEGDPVDVLLGDKLLLALVA
                     ILCVIWVVLIYL"
     gene            747..>1566
                     /locus_tag="PLR57_07265"
     CDS             747..>1566
                     /locus_tag="PLR57_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HQQ41292.1"
                     /translation="MNVYDFDKTIYSGDSTLDFTRFCYRRHPLLLRFLPGQVLAFVPY
                     ALGLIDKTAFKQRFYRYYRGVKDIEVDVRLFWETHKQKIESWYLEQKSESDVIVSASP
                     EFLLRPICAELGVSSLIASRVDSKTGVYDGLNCYGEEKVVRFREVFGEATVERFYSDS
                     LSDAPLAALASESFLVSAGKAVPWAEYKPSKVQKAKRFFLSKEFARFLLIGGINTFNG
                     VLFAYLFSLLLQPNLAFAAGYLLSLSISYLLNSFFTFHESLSFPKYVKFCVSYIP"
BASE COUNT          311 a          455 c          410 g          390 t
ORIGIN      
        1 atggacgtca aaaccgaccg cgaccacccg acaaagcgct tccgccccat cgcaagcggg
       61 gaagtaccaa ttaaaacggc ggccatcctc gcgggaattc ttgtgattgc ttccgccgct
      121 ctgcactact ttgcccatgc ggtgcactgg tatgcctgga tctacctgct cgtttatctg
      181 gcgctcaata tgggctacag cttcgggctc aagaatcagc ccatcatcga tattgcaatc
      241 ctcgtctccg gctttttgat tcgcgtgctc tacggcgcgg cgctcagcgg cattgagatt
      301 tccaactgga tgtacctgac cgtcatcgcc atgagctttt acctcggcct cggcaagcgc
      361 agaaacgaac tcatccgcca gccggacggc gcgcagcgca aggttctgcg gttctacaac
      421 aaagagtttc tcgataaaaa catgtacctt tgcctcgcgc tcaccattgc gttttactcg
      481 ctctggagcg tggaccccat cacgaccgca cgcgtccagt ccaacggcgt cgtctggacg
      541 gtgccgctcg tcatcttcat ctgcatgagc tacagcctgc gcattgaagg caattccgaa
      601 ggcgatccgg tggacgtgct gctgggagac aagctgctgc tggcgctggt ggcaatcctc
      661 tgcgtaatct gggtggtttt gatctacctg taactttaag catccgcgcc gctgataaaa
      721 cgcgcgggtc ggaaagagaa cctgttttga acgtttacga ttttgataaa accatctatt
      781 ctggcgacag cacactggac tttacgcgct tctgttaccg ccgccatccg ctgctgctgc
      841 ggtttctgcc ggggcaagtg ctggcgtttg ttccttacgc gctcggactg atcgacaaaa
      901 cggcctttaa gcagcgcttt taccgctatt accgcggcgt aaaagacatc gaagtggatg
      961 ttcgcctttt ctgggagacg cacaaacaga aaatcgagtc gtggtatctc gagcagaagt
     1021 ccgaaagtga tgtgatcgtc tccgcctcgc cggagtttct gctgcggccc atctgcgccg
     1081 aactcggcgt ttccagcctg attgcttctc gcgtggattc gaaaacaggc gtttacgacg
     1141 gattgaactg ctacggcgaa gagaaggtcg tccggtttcg ggaggtgttc ggagaagcga
     1201 ctgtagaacg cttttacagc gactcgctct cggacgcgcc tcttgccgcg ctggcaagtg
     1261 agagcttcct cgtttccgcc ggaaaagcgg ttccctgggc ggaatataaa ccctccaaag
     1321 tgcagaaagc aaagcgcttt ttcctctcca aggagttcgc gcggtttctt ctcatcggcg
     1381 gcatcaacac cttcaacggc gtgctgtttg cgtatctctt ttcgctgctc cttcagccga
     1441 atctggcgtt cgcggcgggc tacctattga gcctttcgat ctcgtatctg ctcaacagct
     1501 tctttacgtt ccatgagtcg ctgagctttc caaagtacgt caagttctgt gtttcctata
     1561 tcccga
//
LOCUS       DAOMGY010000465         1564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_94745_length_1564_cov_1.827700, whole genome shotgun sequence.
ACCESSION   DAOMGY010000465 DAOMGY010000000
VERSION     DAOMGY010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94745_length_1564_cov_1.827700"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="PLR57_07270"
     CDS             <1..1233
                     /locus_tag="PLR57_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HQQ41293.1"
                     /translation="YQTMLGFGGCFNELGYRALSHLSQADRAELMNELYAPEHANFNL
                     CRLPIGANDYAEGWYSLDETAGDYALAHFSIERDKNCLIPYIKEAQAYQPEMKLFASP
                     WSPPTWMKNPPVYNWGKLVWTPENLNAYARYFARFVQAYAAEGVKIDQVHVQNEPVAN
                     QKFPSCMWTGEELRTFIRDYIGPVLQRECPNTELWLGTINAPGCDFNRLIFDKWASED
                     YDYFANIVLTDEEALQYITGVSYQWGGKIAIQRTFESWWPRIRLMQSENECGFGDNTW
                     DYALYVWTMMKHYIGNGAESYLYWNPVLEPVGRSTWGDPQNAMVTVDPASHRYTLNPD
                     YYVMKSVSAVVQRGAIRLGMKGIWAADTLAFQNPDGSLALSVFNPFHEMRTMTLSYNG
                     ETLSFPLEPRSVNSILIR"
     gene            1269..>1564
                     /locus_tag="PLR57_07275"
     CDS             1269..>1564
                     /locus_tag="PLR57_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylceramidase"
                     /protein_id="HQQ41294.1"
                     /translation="MQAKSTTTIYHNGNITRSTEECILSPDTGSQERDVVNIYPKLVN
                     QQIDGFGGAITESVGYTLSQMPSSTQKEILAAVFGAEGLRYTLVRTSIDSCDFA"
BASE COUNT          376 a          450 c          429 g          309 t
ORIGIN      
        1 tatcaaacga tgcttggctt cggcggctgc ttcaacgagc ttggctaccg cgcgctgtcg
       61 catctttcgc aagccgatcg cgcagaactg atgaacgagc tgtatgcgcc tgagcacgcg
      121 aatttcaacc tctgccgtct tccaatcggc gcaaacgact atgccgaagg ctggtatagc
      181 ctcgacgaga cggcgggaga ctatgcgctc gcgcacttca gcatcgaacg ggataaaaac
      241 tgcctgattc cctatatcaa agaggcgcag gcatatcaac ccgagatgaa gctctttgcc
      301 tcgccctgga gcccgccgac atggatgaag aatccgccgg tctataactg gggcaaactc
      361 gtctggacgc cggagaacct caacgcctat gcgcgttatt tcgcgcgctt tgtgcaggcg
      421 tacgcggcgg agggcgtgaa gatcgatcag gtacacgtgc aaaacgagcc ggtcgcgaac
      481 cagaaatttc cctcgtgcat gtggacgggc gaggagctga gaaccttcat tcgggattat
      541 atcggccccg ttttacaaag agaatgtccg aataccgagc tgtggctggg cacgatcaat
      601 gcgcccggtt gtgattttaa ccgactaatc ttcgataagt gggcaagcga ggactacgac
      661 tacttcgcca acatcgtgct aacggatgaa gaagcgctgc aatacatcac gggcgtaagc
      721 taccagtggg gcggcaaaat cgccatccag cgaacgtttg agagctggtg gccgcgcata
      781 cggctcatgc agtccgaaaa cgagtgcggc tttggcgaca acacgtggga ttacgcgctc
      841 tacgtctgga cgatgatgaa gcactacatc ggcaacggcg cggaaagcta tctctactgg
      901 aatcccgtgt tggagccggt cggacggagc acgtggggag acccgcaaaa cgcgatggtc
      961 accgtcgatc cggcgagcca ccgctacacg ctgaaccccg actattacgt gatgaagagc
     1021 gtctccgccg tcgtgcaacg cggcgcgatc cgcctcggta tgaagggaat ctgggccgcg
     1081 gacacactcg cgtttcaaaa cccggatggt tccctcgcgc tcagcgtatt caatcccttt
     1141 cacgagatgc ggacgatgac gctatcttac aacggagaaa cgctttcctt cccgctggag
     1201 ccgcgctcgg tcaacagcat cctgattcga taacagcagc aatccatcaa gcaacaagga
     1261 gtaccttcat gcaggcaaag tcaaccacca caatctacca taacggtaac atcacgcgtt
     1321 ctaccgaaga atgcatcctt tccccggaca ccggctcgca ggagcgggac gttgtaaaca
     1381 tttacccgaa actcgtcaat cagcaaatcg acgggttcgg cggcgcaatc acggagtccg
     1441 ttggatatac gctctctcaa atgccgtcct ctacgcaaaa agagattctc gccgccgtgt
     1501 tcggcgcgga aggattacga tatacgctcg ttcgaacgtc catcgacagc tgcgattttg
     1561 cgtt
//
LOCUS       DAOMGY010000466         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_94790_length_1563_cov_3.303714, whole genome shotgun sequence.
ACCESSION   DAOMGY010000466 DAOMGY010000000
VERSION     DAOMGY010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94790_length_1563_cov_3.303714"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLR57_07280"
     CDS             <1..505
                     /locus_tag="PLR57_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQQ41295.1"
                     /translation="PVPEPTPTPSLPWYVFAAIGVAAAAVIALVVVLALKNKKKQTNE
                     YVAPSPEPVPSPRPSSAPFKGRQITNVGQASSLGGKSYPVQGKVVIGRDPAKCQVVFP
                     AKTPGISSAHSAVQEIAQGVVVTDLGSSYGTFLENGTKLEPNKPYTVPVGEAFYLASK
                     DNGFRVK"
     gene            529..>1563
                     /locus_tag="PLR57_07285"
     CDS             529..>1563
                     /locus_tag="PLR57_07285"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQQ41296.1"
                     /translation="MESFHTQKLCYHCMQNTVEIGVCRICGCIAEAGQPVPYALPYGT
                     VLHGCYLLGAVLGAGGFGITYIALRLPEGRRVAIKEYFPVEAAFRETGDTVVVSSAGG
                     AEAFRRGREQFLKEAQTIYGLRGYPGIVSVEKLFEENNTAYFCMEFLDGQDLKHRVAQ
                     AGGRLSAAEAMQTFSPVLDALDYIHKREVIHRDVSPDNIFLCRNGGVKLIDFGAAYAR
                     SRDDLSKIQLIVKQGYAPVEQYYQDGNVGPWSDLYALASTMYFCLTGTVPQNALQRAQ
                     RDELMPPTRLGAAIPASVEAAILKGMSVERSGRFSSAAEMKLRLLASAGETRAISGET
                     ASAKSLLFLLR"
BASE COUNT          319 a          451 c          491 g          302 t
ORIGIN      
        1 gccggtgccc gaaccgacgc cgacacccag cctgccctgg tacgtttttg cggcaattgg
       61 tgttgcggcg gctgcggtaa tcgcgctggt cgtcgtgctt gcgcttaaga ataagaagaa
      121 acagaccaac gagtacgttg cgcccagtcc cgaacccgtg ccgtcgccgc ggccttcctc
      181 cgcgccgttc aaaggcaggc agattacgaa tgtcggccag gccagctcgc tgggcggaaa
      241 atcgtacccc gtgcagggca aggtcgtcat cggccgcgat ccggccaaat gccaggtggt
      301 gttcccggca aagacgccgg gcatcagcag cgcgcacagc gccgtgcagg agattgcaca
      361 gggcgtcgtg gtgacggatc tcggctcttc ctacggcacc ttcctggaga acggaacgaa
      421 actcgaaccc aacaagccct atactgttcc ggtcggggaa gcgttctacc ttgcttccaa
      481 ggacaacggt ttccgcgtga aataaaacca cggcggacga ggtaacagtt ggagagtttc
      541 cacacacaaa aactatgcta tcactgcatg caaaacaccg tggaaatcgg cgtttgcagg
      601 atttgcggat gcattgccga agccggacag cccgtgccgt atgctttgcc atacggcacg
      661 gttctgcatg gctgctatct gctcggcgcg gtgcttggcg cgggcggttt tggcattacc
      721 tatatcgcgc tgcgcctgcc ggaaggcaga agggttgcga ttaaggaata ctttcccgtg
      781 gaagccgcgt ttcgtgaaac gggggatacg gtcgttgtca gcagcgcagg cggtgcggaa
      841 gcgttccgcc gcgggcgcga acagttttta aaggaagcgc agacgattta cggcctgcgc
      901 ggctatccgg gaattgtcag cgttgaaaaa ctgtttgagg aaaacaacac agcgtatttc
      961 tgcatggaat tcctcgacgg gcaggatctc aagcaccgcg ttgcccaggc aggcgggcgg
     1021 ctgagcgccg cggaagccat gcagaccttc tcgccggtgc tggacgcttt ggattacatc
     1081 cataagcgcg aagtgattca ccgcgatgtg agcccggaca atatcttttt atgccggaac
     1141 ggcggggtca aactcatcga ctttggcgcg gcgtacgcgc gctcgcggga cgacctgagc
     1201 aagattcagc tcatcgtcaa gcagggctac gcgccggtgg agcagtatta tcaggacggc
     1261 aacgtcggcc cctggtcgga tctgtacgcg ctggcaagca cgatgtattt ctgcctgacc
     1321 ggaacggttc cgcaaaacgc gcttcagcgc gcgcagaggg acgagcttat gccgcccacg
     1381 cggctcggcg ccgcgattcc cgcctctgtg gaagctgcaa tcctcaaagg aatgtccgtg
     1441 gagcggagcg ggcggttttc cagcgcggcg gagatgaaat tgaggctgct tgcctccgcg
     1501 ggagaaacgc gcgccatctc cggcgaaacg gcctccgcaa agtcgcttct ttttcttctg
     1561 cgg
//
LOCUS       DAOMGY010000467         1561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_95002_length_1561_cov_2.953519, whole genome shotgun sequence.
ACCESSION   DAOMGY010000467 DAOMGY010000000
VERSION     DAOMGY010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95002_length_1561_cov_2.953519"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..709)
                     /locus_tag="PLR57_07290"
     CDS             complement(185..709)
                     /locus_tag="PLR57_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HQQ41297.1"
                     /translation="MNILLTGGSACGKSTFGEALAARLPKPRTYVATMRPFEGESEEK
                     ILRHQFQRADKGFSTIERETDLSGLRLPARGTVLLECVCNLAANEMFDDTGNIRPGAE
                     EAILSGIDKLSQQCETLIVVTNDVGSDGGGYDESTMRYVDLVGRVNRELASRFDCVCE
                     LVCGIPLVIKGFLP"
     gene            complement(827..>1561)
                     /locus_tag="PLR57_07295"
     CDS             complement(827..>1561)
                     /locus_tag="PLR57_07295"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HQQ41298.1"
                     /translation="RCANADVIPVDMGMFRTVAEKNLLDRRVASGTADMTSGPAMTRK
                     QAEQAIEAGIELVRSCKEQGYRILATGEMGIGNTTTSSAIASVLLSRDPFEMTGRGAG
                     LDDEGLERKISAIRCAIEVNQPDPEDALDVLAKLGGFDIAGLCGVFLGGALYGVPVLI
                     DGFISATAALVAARICPNANAYMLPSHASAEPAAKYVLDELRVLPILQANMRLGEGTG
                     AVAVLPLLDMALAVYRELMTFADIGM"
BASE COUNT          323 a          495 c          431 g          312 t
ORIGIN      
        1 ggagagcccc aatcagcacg ccgaccagcg gaagcgcggc aagcgcaaaa cgcatgttct
       61 ccggtttcca cgccaccttc ggcatgggta cgcggctgaa catgagaaac gccgtcgcta
      121 tggatcgcaa aactgtcatt gcagcttcct ttcaaaaaca ctgttgtgat gggttgccgt
      181 ctgtttacgg caaaaagcct ttgatcacaa gcggaatgcc gcagacgagc tcgcagacgc
      241 agtcgaaccg gctcgcaagc tcgcggttga cgcgcccgac cagatcgaca taccgcatgg
      301 tgctttcgtc atatcccccg ccgtcgctgc cgacgtcgtt ggtcacgacg atcagcgttt
      361 cgcactgctg gctcagtttg tcgataccgc taaggattgc ctcctccgcg ccggggcgga
      421 tattgcccgt gtcgtcgaac atttcatttg cggcaaggtt gcagacgcac tccagcagaa
      481 ccgttccccg cgcgggcagg cgcaaaccgg aaagatccgt ttcccgctcg atcgtcgaaa
      541 agcctttgtc cgcgcgctgg aactgatgac ggagaatctt ttcctcgctt tcgccttcga
      601 acgggcgcat ggtggcaacg taggttctcg gcttcggcag ccgcgcggcg agcgcttcgc
      661 cgaaggtgct cttgccgcag gcggaaccgc cggtcagcag gatgttcaat tcgctcgcct
      721 cccacctaag actttttctc tgcttttctt gaaaagaaaa gcagcaaaag aacctctatc
      781 ctaaagttac tttgctaacg aaagaacaat aaacaaaaga acttctttac atgccgatat
      841 cggcaaacgt catcagctcg cgatagaccg cgagagccat atctaaaagc ggtagcaccg
      901 ccaccgcgcc cgtgccttcg ccaagacgca tattcgcctg cagaatcggc agcacacgca
      961 gttcgtcgag aacgtatttt gccgcgggct ccgcggaagc atggctcggc aacatgtaag
     1021 cattcgcgtt cgggcaaatg cgcgcggcaa caagcgccgc cgtcgcgctg atgaatccgt
     1081 cgatcagtac gggcacgccg tacaacgccc cgcccagaaa caccccgcaa agccccgcaa
     1141 tgtcgaaccc gccgagcttt gccagaacgt ccaacgcatc ctctgggtcg ggctggttga
     1201 cttcgatggc gcaccggatg gcggaaatct tccgctccag tccttcgtcg tcgagccccg
     1261 cgccgcgccc ggtcatttca aacgggtcgc gcgagagcag cacggaagca atcgcgctgg
     1321 aagtcgtcgt gttgccgatt cccatttcac cggtcgctaa aattcggtag ccctgctcct
     1381 tgcaggaacg gaccagctcg attcccgctt caatcgcctg ttcggcctgt ttgcgcgtca
     1441 tggcggggcc tgaggtcatg tccgccgtgc cggaagcgac cctgcggtcg agcaggtttt
     1501 tctccgctac tgtgcggaac atgcccatgt ccacggggat gacgtccgca ttggcgcagc
     1561 g
//
LOCUS       DAOMGY010000468         1560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_95091_length_1560_cov_2.790033, whole genome shotgun sequence.
ACCESSION   DAOMGY010000468 DAOMGY010000000
VERSION     DAOMGY010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95091_length_1560_cov_2.790033"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..>1560
                     /locus_tag="PLR57_07300"
     CDS             141..>1560
                     /locus_tag="PLR57_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HQQ41299.1"
                     /translation="MELHGIVTLPVIPLRGIAVLPGEIMHCDIGRKKTLSAMEQALSE
                     EGYALFVAQKNAKVVDVAPDDLYSVGTICRIRQVFRIQNDTVHLLVTGVARARIESVI
                     SENPHYIAQVAVLEDTEPDEATVEAFRRRLSEGFTEHASQRDRLTQDQKDAILSLPTL
                     SELTDSIAQQVVGKLEDKQKLIEEADLVTRAASLLSIIENELLVMQVDRRIAGKIKAA
                     LERNQKEFVLREQLKAVQEELGEGDEDEAEIYRERALKKKLPDEVRTVLNKQIERFAS
                     LPGGSHEAPMAQAYIELILDLPWMESTEDNLDLKHAREVLDRDHFGMERVKTRIIESL
                     AVQRLTNNPQGQILCLVGPPGVGKTSIVRGIAEAMGRKFIRMSLGGVHDEAEIRGHRR
                     TYIGAQPGRVIEAMRKAGSINPVLLFDEIDKLGADVRGDPSSAMLEVLDGAQNFAFVD
                     HFLELPFDLSRSMIVTTANDPGA"
BASE COUNT          353 a          444 c          463 g          300 t
ORIGIN      
        1 cgaacaccta tagtgtggca tggtcaatcc cacgagaata atgattgacc ccaaatttgc
       61 ataatgcaaa caagtccgtt atacttgtca tacgatcatg gggcactcta cagtttcagt
      121 ccctgacgga ggtgaaatat atggaactgc atggaatcgt aaccctgccg gtcatcccgc
      181 tgaggggtat tgccgtactg ccgggcgaga ttatgcattg cgatatcggg cgcaaaaaaa
      241 cgctgtccgc tatggaacag gcgctgtccg aagaaggata cgctttgttc gttgcacaga
      301 aaaacgccaa ggtagtggac gtcgctcccg acgacctgta ttccgttggc accatctgcc
      361 gcatccggca ggtgtttcgc attcagaacg acaccgtgca ccttttggtg acgggtgttg
      421 cgcgcgcgcg cattgaaagc gtcatttccg aaaacccgca ctacattgcg caggttgccg
      481 tgctggaaga tacggagccg gacgaagcga ctgtggaagc gttccgccgc cgcctcagcg
      541 agggttttac cgagcatgct tcgcagcgcg accgccttac gcaggatcag aaagatgcaa
      601 tcctctcgct gccgacgctt tcggaactca cggactccat cgcccagcag gtggtgggta
      661 agctcgagga caagcagaag ctcattgaag aggcggatct tgtgacccgt gctgcgagcc
      721 tgctttccat catcgagaac gaactgctcg tcatgcaggt cgaccgccgc atcgcgggca
      781 agatcaaagc ggctttggaa cgcaaccaga aggaattcgt cctgcgcgag cagctcaagg
      841 ccgtgcagga agagcttggc gaaggcgacg aagacgaggc ggaaatctac cgcgaacgcg
      901 cgctcaaaaa gaagctgccg gacgaagtcc gaacagtatt gaataagcag atcgagcggt
      961 ttgcgtctct tccgggcggc tcgcatgaag cgcccatggc gcaggcgtat atcgagctga
     1021 ttctcgattt gccgtggatg gagtccacgg aggacaacct cgatttgaag cacgcgcggg
     1081 aagtgctcga ccgcgaccac ttcggcatgg aacgcgtgaa gacgcgcatc attgagagtc
     1141 ttgccgtaca gcgtctgacc aacaacccgc aggggcagat tctctgcctc gtgggccctc
     1201 cgggcgtcgg caaaacgtcc attgtgcgcg gtattgcaga ggctatgggc agaaagttca
     1261 tccgcatgag cctgggcggc gtgcacgacg aagcggaaat ccgcggacac cggcgtacct
     1321 atatcggcgc gcagcccggc cgggtcattg aagccatgcg caaggcgggt tccatcaacc
     1381 ccgtgctgct gttcgacgaa atcgacaaac tcggcgcgga cgtgcgcggc gatccttcgt
     1441 ccgccatgct ggaagtgctc gacggcgcgc agaacttcgc gttcgtggat catttcctcg
     1501 aactgccgtt cgacctttcg cgcagcatga tcgttacgac cgcaaacgat cccggcgcca
//
LOCUS       DAOMGY010000469         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_95161_length_1559_cov_3.380984, whole genome shotgun sequence.
ACCESSION   DAOMGY010000469 DAOMGY010000000
VERSION     DAOMGY010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95161_length_1559_cov_3.380984"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLR57_07305"
     CDS             <1..364
                     /locus_tag="PLR57_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQQ41300.1"
                     /translation="DYVPMLFISAKNGQRVGKVMEAVVSAFEQSRRRITTGQLNDTVS
                     EAIMMNVPPSDKGRNLKIYFATQASVQPPTFIIFVNDTEIVHFSYERYMENFFRKSFG
                     FEGTPIRLIFRNRKKDSE"
     gene            364..993
                     /locus_tag="PLR57_07310"
     CDS             364..993
                     /locus_tag="PLR57_07310"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HQQ41301.1"
                     /translation="MTFFTGLWTLGLWTVLACVFAGYLLGNIQTAIMVSKWYYHDDVR
                     NHGSGNAGSTNMVRVFGFGPGAITFAGDFTKALLSVLVGQLICGTVGGYIAGLFVVVG
                     HCWPMFAGFRGGKGVASSASLMIFTFPLGAGIALAIGAIILSINKRVSIVSMSSILLF
                     FICTLIFRLNDLPLVLLAGLLAIIVYVRHIENIKRILHGEEQRLRKEGR"
     gene            complement(1058..>1559)
                     /locus_tag="PLR57_07315"
     CDS             complement(1058..>1559)
                     /locus_tag="PLR57_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41302.1"
                     /translation="SGLYNMKNVVSEHDAASTLLSLEDQAVVSVLGSSRDESLLSSLF
                     DAVAERGVFVDMIACGEPGDSLSIGFSLASDDLCQALEALNAFRSSGVDFSFRTRSDL
                     TKLTLENSGMALKHGVAAQLLSALNKASIHVWLITTSETRIEFCVDAVDAHKAVEEVK
                     SKFGIR"
BASE COUNT          325 a          420 c          432 g          382 t
ORIGIN      
        1 ggattatgtg cctatgctgt ttatctcggc caaaaacggc cagcgcgtcg gcaaagtgat
       61 ggaagccgtc gtttccgcgt ttgagcagag tcgccgccgc attacgaccg gtcagctcaa
      121 cgataccgtc agcgaagcga tcatgatgaa cgtgccgcct tccgacaaag gccgaaacct
      181 taaaatctac tttgccacgc aggcgagtgt acagccgcca acgtttatca tctttgtcaa
      241 cgataccgaa atcgtgcact tttcttacga gcgctatatg gaaaacttct ttcgtaagtc
      301 gttcggtttc gaggggacgc cgatacggct tattttccgc aacagaaaga aggactccga
      361 ataatgacgt ttttcactgg actttggacg cttggcctct ggacggttct ggcatgtgtt
      421 ttcgcaggtt atctgctcgg caatattcaa acggcaatca tggtcagcaa atggtactat
      481 catgacgacg ttcgcaacca cggttccggc aacgcaggca gcaccaatat ggtgcgcgtg
      541 ttcggtttcg ggccgggcgc gattacgttt gcgggagact ttaccaaagc gcttttgagc
      601 gttctggttg ggcagctgat ctgcggcacg gtcggcgggt atatcgctgg gctgttcgtc
      661 gttgtcggtc actgctggcc gatgttcgcg ggcttccgcg gggggaaagg cgttgcttcc
      721 tccgccagcc tgatgatctt tacgttcccg ctcggcgcgg gcattgcgct tgcgattggg
      781 gcgatcatcc tctccataaa taagcgcgtg tcgatcgtct ctatgtcgag cattctctta
      841 ttctttatct gcacgctgat tttccggctc aacgatctgc cgctggtgct gctggccggg
      901 ctgctcgcaa tcatcgtgta cgtgcgccat atcgaaaaca tcaagcgcat tctgcacggc
      961 gaagagcagc gccttagaaa agaaggacgt tagttcctcc aaaacacaaa gcgcttcccg
     1021 ttatctgtac gggaagcgct ttttcttgtg aaattgctta acgaatgccg aattttgatt
     1081 tcacttcttc aacggctttg tgcgcatcca ccgcgtccac acagaactcg atgcgcgttt
     1141 cgctggtggt gatgagccaa acatggatgc ttgctttgtt cagcgcggag agcagctgtg
     1201 ccgcaacacc gtgcttcaac gccatgccgg aattctccag agtgagcttg gtcaagtccg
     1261 aacgcgtgcg gaaggagaaa tctactccgg agctcctgaa cgcgttgagc gcttccagcg
     1321 cttggcatag atcgtcgctg gcgagagaaa agccgatgct gagactgtcg ccgggttcgc
     1381 cacaggcgat catgtccacg aatacaccgc gctcggcaac cgcgtcgaac aggctcgaca
     1441 aaagtgattc gtccctggaa gagccgagta cgctgaccac agcctgatct tccagcgaaa
     1501 gcagggtgga agccgcgtcg tgttcgctga cgacgttttt catgttgtac agtccgctg
//
LOCUS       DAOMGY010000470         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_95209_length_1559_cov_2.384973, whole genome shotgun sequence.
ACCESSION   DAOMGY010000470 DAOMGY010000000
VERSION     DAOMGY010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95209_length_1559_cov_2.384973"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLR57_07320"
     CDS             <1..193
                     /locus_tag="PLR57_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41303.1"
                     /translation="DGKRLRECLEFHIVLLTVRVCPVEACADDDSSTEYNIFSAPLSN
                     GKGNRILCFAVFDENKTPS"
     gene            342..1403
                     /locus_tag="PLR57_07325"
     CDS             342..1403
                     /locus_tag="PLR57_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQQ41304.1"
                     /translation="MKKQVSINDVAQEAGVSAATVSYVLNNSPVNLRPETREKVLMAV
                     KTLKYVPNQAAKTLGSSRVKGNGASKLIGVVIPQTEPGKYFMFSNPFYGDFLSMVEYE
                     ARKASFHILISGVNAEESYVSIAKNRSLDGVIIVGMYPSADIAEYRKAKIPVVLVDCY
                     CNEDHVFHSVRTDDRQGGYLATNHLIQNGHRRIAFISGQVKDSGVTQMRYLGYQDALR
                     EAGIPLDKSLLFDGTVDFQYGREAAMRISEANAGQKDPITAVFATSDIAAVGAIKGFE
                     QSGLTVPGDISIVGFDDVYYAELSNLTTIRQNINEKGREAARILIAAAEDAKLSKRER
                     IIPLELVERASVRRIGSVE"
BASE COUNT          377 a          428 c          432 g          322 t
ORIGIN      
        1 agacggcaaa cgcctgcggg agtgccttga gttccatatt gtcctcctta ccgtgcgtgt
       61 gtgccctgtg gaagcttgcg cggacgatga ttcgtcaacc gaatataaca ttttcagcgc
      121 gcctttgtcc aacggaaagg gtaatcgtat tctctgcttt gcagtttttg atgaaaacaa
      181 aactccctct tgacatgcgg ctcgaaatgc gatactcttc gtttaagcgg ttaaacaacc
      241 gcaaagccaa tcagaagcca tacggtcggc aaagaagtca atcaagtaaa aaaatcaaac
      301 agatcggcag ccgtagtgaa tacggcgaag tgggaagcgg aatgaaaaag caggtcagca
      361 tcaatgacgt tgctcaagag gcgggcgttt cggccgccac cgtttcctat gtgctcaaca
      421 actcacccgt caatctgcgg ccggaaacgc gcgaaaaagt gctcatggcg gtcaaaacgc
      481 tcaagtacgt gcccaaccaa gcggcaaaaa cgctgggttc aagccgcgtc aagggcaacg
      541 gcgcttccaa gctcatcggc gtggtgattc cgcagacgga acccggcaag tacttcatgt
      601 tcagcaaccc gttttacggc gattttctga gcatggtgga gtacgaagcg cgcaaggctt
      661 ccttccatat cctcatctcc ggcgttaacg ccgaggaaag ctacgtcagc atcgccaaaa
      721 accgctcgct ggacggcgtg atcatcgttg gcatgtaccc ttcggccgat atcgccgagt
      781 accgcaaggc gaagattccc gtggtgctgg tggactgcta ctgcaacgag gaccacgtgt
      841 tccacagcgt ccgcacggac gaccgtcagg gcggttacct tgcaacgaac cacttaattc
      901 agaatggcca ccgccggatt gcgtttattt ccggacaggt caaggacagc ggagtcacgc
      961 agatgcgcta tcttggctat caggacgcgc tgcgcgaggc gggcattccg ctggataaat
     1021 cgctgttgtt cgacggcacg gtggactttc agtatggccg cgaagcggca atgagaattt
     1081 ccgaagcaaa cgcgggccaa aaagacccca ttaccgccgt atttgccacc tcggacatcg
     1141 ccgccgtcgg cgcgatcaag ggctttgagc agagcggact tacggtgccg ggagacattt
     1201 ccatcgtcgg tttcgacgac gtgtattacg cggaactttc taatctgacc accatccggc
     1261 agaacatcaa cgaaaaaggg cgcgaagccg cgcggatcct cattgccgcg gcggaggacg
     1321 cgaaactttc caaacgcgaa cgcatcatcc cgctggagct cgttgaacgc gcttcggtca
     1381 ggcggattgg ttcggtggaa taatcagatc attcagactc tatttcattt cgggagcagg
     1441 aactcatggc gatcaaacac caaccatacg gattgtttcc gcatttcgac agcggcatgg
     1501 agcgcaggcc ctactttgtt acggcagggt cggaagcggc gctcggctgc cggtttgat
//
LOCUS       DAOMGY010000471         1558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_95235_length_1558_cov_4.567532, whole genome shotgun sequence.
ACCESSION   DAOMGY010000471 DAOMGY010000000
VERSION     DAOMGY010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95235_length_1558_cov_4.567532"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLR57_07330"
     CDS             <1..363
                     /locus_tag="PLR57_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQQ41305.1"
                     /translation="QIRDAGFTDTEHAICTFLCFHDSVSQDAIANALLLDKTTVAKSL
                     SAMEAKGLVVRKQNERNRRMNIIQISEAGKASVSGSIHLYDEWLAELCSCLSPEEQRQ
                     MDDYFTRIIASAVKRREK"
     gene            392..658
                     /locus_tag="PLR57_07335"
     CDS             392..658
                     /locus_tag="PLR57_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41306.1"
                     /translation="MTQNAAQKQPSPFITVLKKIGSGLKAYLTDWKNLLGHALLGVFF
                     VVIAVWAPINLWLKLGIIACLIGLNILRMRLKAKKQAEKERIPE"
     gene            655..>1558
                     /locus_tag="PLR57_07340"
     CDS             655..>1558
                     /locus_tag="PLR57_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41307.1"
                     /translation="MSSFKDLRIVDNFYQTSAFYPMPTILVSTLAEDDQTSLGSYSLC
                     FPYYVAGKDYYAMLLETRNSSNTAQNILRSGTCALNFIEDSRANFKEAVRLGFPGETC
                     AEKMKGCRFTLEEGQAGGEQKRPLVVKEAYQVMECTWMSDLEGASEDSARVGQLEGME
                     PPYRSFNGVTSKFGAHFILRVDKILMKERFYNAIVGGVKANSFPRVPVDYGYRDNTHF
                     WYTRFTRPLSERIAAGKEAELSTVIYAASRVDPNVKFTDAACQTLVKVPRVFLKAALQ
                     GCVDWAKENGVSVLDVEHMAIIRDK"
BASE COUNT          373 a          464 c          435 g          286 t
ORIGIN      
        1 caaatccgcg acgcgggctt tacggatacg gagcacgcca tctgcacgtt cctgtgcttt
       61 cacgacagcg tttcgcagga cgcaatcgcc aacgcgctgc tgctggacaa aaccacggtg
      121 gcaaaatcgc tcagcgcaat ggaagccaag gggctcgtcg tccgcaaaca gaacgagcga
      181 aaccggcgca tgaacatcat ccagatttcc gaagcgggca aggcaagcgt cagcggcagc
      241 atccatcttt acgacgagtg gctcgccgag ctctgctcct gcttaagccc cgaagaacag
      301 cgtcagatgg acgactactt tacccgcatc atcgcctccg ccgtcaagcg acgggagaaa
      361 taatcgaacc tcaatcgaaa ggattttatc aatgacgcaa aacgcagcgc aaaaacagcc
      421 ttctccgttt atcacagtac tcaaaaaaat cggaagcggg ctgaaagcct acctgaccga
      481 ctggaagaac cttctggggc acgccctgct cggcgttttc tttgtggtca tcgccgtctg
      541 ggcgcccatc aacctctggc tcaaactcgg catcatcgcc tgtctgatcg gtctgaacat
      601 cctcagaatg cgcctgaaag ccaaaaagca agcggaaaag gagcgtatcc cggaatgagt
      661 tcgtttaaag atcttcgcat cgtcgataac ttctaccaga cctcggcgtt ttaccccatg
      721 ccgaccatcc tcgtttccac gctggcggag gacgaccaaa cgtcgctggg ttcctactcg
      781 ctgtgctttc cgtattacgt ggcgggcaag gactattacg ccatgctgct ggaaacgcga
      841 aacagctcca acaccgcgca gaacatcctg cgcagcggca cgtgcgcgct gaacttcatt
      901 gaggacagcc gcgccaactt caaggaagcg gttcgactgg gctttcccgg ggaaacctgc
      961 gccgaaaaga tgaagggctg ccgtttcacg ctggaagagg gtcaagcggg aggcgaacag
     1021 aagcgcccgc tcgtggttaa ggaagcgtat caggtaatgg aatgcacctg gatgtccgat
     1081 ctggagggcg caagcgaaga ttccgcgcgc gtaggccagc tcgaaggcat ggaaccgccg
     1141 taccgcagtt tcaacggcgt taccagcaag ttcggcgcgc atttcatcct gcgcgtggat
     1201 aaaatcctga tgaaggaacg cttctacaac gccatcgtcg gcggagtcaa ggcgaacagc
     1261 ttcccgcgcg ttccggtgga ctacggctac cgcgacaaca cgcatttctg gtacacgcgc
     1321 tttacgcgcc ccctctcgga gcgcattgcc gcgggcaagg aagcggagct ttccaccgta
     1381 atctacgccg caagccgcgt cgatccgaac gtaaagttta cagacgccgc ctgccaaacg
     1441 ctggtcaagg tgccgcgcgt gttcctcaaa gccgcgctgc agggctgcgt ggactgggca
     1501 aaagagaacg gcgtgagcgt gctggacgtg gagcatatgg caattatccg cgacaagc
//
LOCUS       DAOMGY010000472         1558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_95239_length_1558_cov_4.121091, whole genome shotgun sequence.
ACCESSION   DAOMGY010000472 DAOMGY010000000
VERSION     DAOMGY010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95239_length_1558_cov_4.121091"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..886)
                     /locus_tag="PLR57_07345"
     CDS             complement(152..886)
                     /locus_tag="PLR57_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HQQ41308.1"
                     /translation="MRVIVTENFEQTCEIAAGMIADVIRKKPDARLGLATGSTAQGVY
                     PYLVRANQMGEVDFSRVNTVNLDEYVGLAADHPQSYRRFMDENLFDHINIDKKNTVVA
                     KGTGDIEANVREFREAIAKKPIDVQLLGIGVDGHVGFNEPGEVLYDGAHVEELDESTI
                     DANSRFFASREEVPTRAISMGMGDILRAKKLLLLATGENKTEAMRGLLLNEELDAENP
                     ATLVKTHPDVTVIIDKALAKSVGYSY"
     gene            complement(945..>1558)
                     /locus_tag="PLR57_07350"
     CDS             complement(945..>1558)
                     /locus_tag="PLR57_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQQ41309.1"
                     /translation="RYEKFKRAVELDVMRRAESVREEIDFALSLEAEAGRLHRMRQSC
                     ELPTRVTHNDTKINNVLMDRYSRKALCVIDLDTVMPGLAAYDFGDSIRFGAATAAEDE
                     LDLSLMRLDVELFETFSRGFLQACTSLTPLEVETLPLGAFTMTLECGVRFLTDYLEGD
                     HYFKIKREQHNLERCRTQFTLAADIRRHLPKLKHIVLMHAGNR"
BASE COUNT          296 a          460 c          421 g          381 t
ORIGIN      
        1 cgcccgtcgc ttccacggcg atggagaccg ggcgctttgc cttcacttca aaggcgttca
       61 tccaaggccc catagggata aagccatgat cgagttccgg caggttcttg agttcaaagt
      121 cgattcccag ataccgcatt ccgcttctcc cttagtagct gtagccaacg ctcttggcaa
      181 gcgctttgtc tatgataacc gtcacgtccg ggtgcgtctt aaccagcgtc gcggggtttt
      241 ccgcgtccag ttcttcgttc agcagcagtc cccgcatcgc ttcggttttg ttctcgcccg
      301 tagcgagcaa taggagcttc tttgctctga gaatatcgcc catgcccatg gagatcgccc
      361 gcgtcggaac ttcctcacgg gaggcgaaaa agcgggaatt ggcgtcgatg gtgctctcgt
      421 ccagctcctc aacatgcgca ccgtcgtaga gaacttcacc cggctcgttg aagccgacgt
      481 ggccgtccac gccgatgccc aagagctgca cgtcgatggg ctttttggca atcgcctcgc
      541 ggaattcacg aacgttcgct tcaatgtcgc ccgtaccttt ggcgaccacg gtgttctttt
      601 tgtcgatatt gatgtggtcg aataggtttt cgtccataaa tctccggtag ctctgcggat
      661 gatccgccgc gagtccgacg tactcatcaa ggttgacggt gttgacgcgc gaaaaatcga
      721 cttcgcccat ctgatttgct ctcacaaggt aaggataaac gccctgcgcg gtgctgccgg
      781 tggcaagccc cagccgcgcg tccggctttt tacggataac gtcggcaatc atgccggcgg
      841 cgatttcgca ggtctgctca aagttttcgg ttacgatgac tctcatggtg tttcaatcct
      901 ctttccggtg attatgaatt cttgttccgc tgttcagatt gcgcttatcg gttgccagcg
      961 tgcatcagga cgatatgctt gagttttggc aagtgccgcc ggatatccgc cgccagcgta
     1021 aactgcgtgc ggcagcgctc taaattgtgc tgctcgcgtt tgattttaaa gtaatggtcg
     1081 ccttccagat aatccgttaa aaaccgcacg ccgcactcca gcgtcatggt aaatgcgccg
     1141 agcggcaggg tttcgacctc cagcggagtc aggctcgtgc aggcctgcaa aaatcccctg
     1201 ctgaacgttt cgaacaactc cacgtccagc cgcatcagac tcaaatcgag ttcgtcctcg
     1261 gcggcagttg ccgcgccaaa acggatggag tcgccaaagt cgtatgccgc aagtcccggc
     1321 atgaccgtat cgaggtcgat gacgcaaagc gccttgcggc tgtagcgatc catcagaacg
     1381 ttgttgatct ttgtatcgtt gtgcgtcacc cgcgtcggca gttcgcagct ttggcgcatg
     1441 cggtgcagcc tgcccgcctc ggcttccagc gagagcgcaa agtcgatctc ttcgcgaacg
     1501 ctctcggcgc gccgcatgac gtccagttcg acagcacgtt taaacttttc gtatcgcg
//
LOCUS       DAOMGY010000473         1554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_95668_length_1554_cov_2.771848, whole genome shotgun sequence.
ACCESSION   DAOMGY010000473 DAOMGY010000000
VERSION     DAOMGY010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95668_length_1554_cov_2.771848"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLR57_07355"
     CDS             <1..261
                     /locus_tag="PLR57_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41310.1"
                     /translation="GTSGKWYKITVVSTGKTGYVYATYVTLTPATPTPTPTPTPTPTP
                     TATPTASPTATPTASATITPSAPASVSPSSSVSPSSSVSPAS"
     gene            complement(323..1264)
                     /locus_tag="PLR57_07360"
     CDS             complement(323..1264)
                     /locus_tag="PLR57_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HQQ41311.1"
                     /translation="MMKIFKSEGPTMSEIKAPEKDCWISLVRPTEVELASIAQLYEID
                     PDDLKAALDEEERSRIEVEDTYTLVLVDIPAVEKHKGKDIYTTMPLGILLAKDAIITV
                     CLEETPVLHSLKQKHANELRTNMRTRFILQILYRNASLYLQYLRVINKLSDNVEQKLH
                     GSTQNHELIELLELEKSLVYFTTSLRSNEVVLEKLLKTERIKKYPEDEDLLEDVIVEN
                     RQAIEMANIYSGILSGMMDAFASVISNNLNIVMKFLTIITIVLSVPTIIFAAYGMNLV
                     PEGMPLAKSPYGFLVIVLVSFVISIGVAVFFAKKKMY"
     gene            complement(1284..>1554)
                     /locus_tag="PLR57_07365"
     CDS             complement(1284..>1554)
                     /locus_tag="PLR57_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41312.1"
                     /translation="IARNRRCRQEKTAVSVRSGRYISGLRVFPQKESHCLQNSGFAGL
                     KKATIMKLRIGKESDWTVDTVPERSRKTISYFARVREVTLFRRAR"
BASE COUNT          332 a          415 c          417 g          390 t
ORIGIN      
        1 ggcacctccg gcaagtggta taagattacg gtcgtctcga ccggcaaaac gggctatgtg
       61 tacgcgacct acgttacctt gacacccgca acgccgactc ctacgcctac gccgaccccg
      121 accccgacgc caactgcgac gccgaccgct agcccaaccg ccactccgac cgcctcggcg
      181 accataacgc cgtccgcgcc ggcatcggtt tcgccgtcga gttcggtttc gccttcgagt
      241 tcggtttcgc cggcatcctg aaaaacgaat ctatagcaaa gaagctcccg caagggagct
      301 tcttgttatg catactagaa tttcaataca tcttcttctt ggcaaagaac accgcaaccc
      361 cgatggagat gacgaacgaa accagcacga tcaccagaaa tccgtacggg ctttttgcca
      421 gcggcatccc ttcgggaaca aggttcatgc cgtacgcggc aaagatgatg gtcggcaccg
      481 aaagaacgat cgtgatgatg gttaagaact tcattacgat gttcaggttg ttcgaaatga
      541 cggaggcgaa cgcgtccatc atgccgctga gaatgccgct gtagatattg gccatttcga
      601 tggcctgcct attttcaacg atgacgtctt cgagcaagtc ctcgtcctcc ggatactttt
      661 taatccgctc ggttttgagg agtttttcga gcacgacttc gttggaacgg agcgatgttg
      721 taaagtagac gaggctcttt tccagctcca gcagttcaat gagctcgtgg ttctgggtgg
      781 aaccgtggag cttctgctca acgttgtcgc tgagcttgtt gatcacgcgg aggtactgca
      841 gatagagcga cgcgttgcgg tacagaatct gcagaatgaa gcgcgtgcgc atgttggtgc
      901 gcagctcgtt tgcgtgcttc tgcttgaggg aatgcagaac cggcgtttcc tccagacaga
      961 cggtgatgat ggcgtccttt gccaagagga tgccaagcgg catcgtggtg tagatatctt
     1021 tgcctttgtg tttttcaaca gcggggatgt ctacgagtac gagggtatag gtatcctcga
     1081 cttcaatacg agagcgttct tcttcgtcca gcgcggcctt caggtcgtcg ggatcgattt
     1141 cgtagagctg tgcgatgctg gcaagctcca cctccgtggg ccgcacaagg gaaatccagc
     1201 agtctttttc aggcgctttg atctcgctca tggttggacc ttcggatttg aagattttca
     1261 tcatggcggt attctccttt cgcttaccgc gcgcggcgga aaagcgtcac ttcccgaacg
     1321 cgcgcgaaat atgatatggt ttttctactt cgttcgggaa cggtgtccac cgtccaatca
     1381 ctctcctttc cgattcgtag cttcattata gtcgcttttt tcagccctgc aaagcctgaa
     1441 ttttgaagac aatgtgattc tttttgcggg aaaacgcgta aaccggagat atatctgccg
     1501 ctccggacgg aaacggcggt tttttcttgg cggcaacggc gattacgggc aata
//
LOCUS       DAOMGY010000474         1553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_95743_length_1553_cov_3.350467, whole genome shotgun sequence.
ACCESSION   DAOMGY010000474 DAOMGY010000000
VERSION     DAOMGY010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95743_length_1553_cov_3.350467"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..1062
                     /locus_tag="PLR57_07370"
     CDS             31..1062
                     /locus_tag="PLR57_07370"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQQ41313.1"
                     /translation="MYDYFQKTPLDETFYTRFLKDRLPARLIDAHAHYDLPEHVKNVT
                     AEAVAGDWALECGLLMSYEDACAYYRTLLPDTGVYKVALPWPLRDVDTHANNAYVSRL
                     TEHAEVRSLLTTRPEYDTALVERTFRNGRFSGFKPYPYMASATKGAEVSIFDFFTHDQ
                     FALANKLGAPVLMHLPRAGRLPAPENVAEIRTILDRYPNVKLVVAHFGRCFNVEYLER
                     ALELFGEDIHRFCFDTAAVLNPAVHKLALHELDYRNILFGTDFPILLWHGKREWKNGT
                     YINLCRENFGWNKHYYPEDEPNYTFFVYEQLKNMLDLIGDDEEKKKAIFFENAKRVYF
                     DYPQGGQQA"
     gene            1059..>1553
                     /locus_tag="PLR57_07375"
     CDS             1059..>1553
                     /locus_tag="PLR57_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HQQ41314.1"
                     /translation="MNKVLAKHLGNMDQLAGVRLSRLESGNAAGKRIAEVYNAAGLRF
                     TVLPDKCMDLFDLSYKGVNIAFHSKNALSAGEPFYPVPSEFFGYWSGGMLATCGLANI
                     GGADESDPTCVHPIHGRIGYQSADCFGYDCAWQGEDYILSLEGRMRETRLYGRSLELR
                     RRIRT"
BASE COUNT          338 a          471 c          435 g          309 t
ORIGIN      
        1 gtgccgttcc ggcagaaagc gtgacaggac atgtacgact attttcagaa aacaccgctg
       61 gacgaaacct tttacacccg gttcctcaag gaccgcctcc ccgcgcggct gatcgatgcg
      121 cacgcgcact acgacctgcc cgaacacgta aaaaacgtca ccgcggaagc cgttgcgggc
      181 gactgggcat tggaatgcgg gcttttgatg agctatgagg acgcctgcgc atattaccgc
      241 accctcctgc cggataccgg cgtttataaa gtagcgctcc cctggccgct cagggatgtg
      301 gatactcacg ccaacaacgc ctacgtttca cgcctcacgg aacacgcgga ggtgcgttcg
      361 ctcttgacca cacggccgga atacgatacg gcgttggtcg aacggacgtt ccggaacggg
      421 cgtttttccg gttttaagcc ttacccatac atggcgagcg cgaccaaggg cgcggaggta
      481 agcatcttcg attttttcac ccacgatcag ttcgcgcttg ccaacaagct cggcgcgccg
      541 gtgctcatgc atctgccccg cgcgggaagg ctccccgcgc cggaaaacgt tgccgaaatc
      601 cggacaattc tggatcgcta cccgaacgtc aagctcgtgg tcgcgcactt cggcaggtgc
      661 ttcaatgttg agtaccttga acgcgcgctg gagctgttcg gcgaggatat ccaccgcttc
      721 tgctttgata cggctgcggt gctcaacccc gctgtgcaca agctcgcact acacgaactg
      781 gactatcgta acatcctctt cggcacggac ttccccatcc tgctctggca cggcaaacgg
      841 gaatggaaaa acggaacgta catcaacctc tgtcgcgaga atttcggctg gaacaagcat
      901 tattatcccg aggacgagcc gaactacaca ttctttgtct acgaacagct caagaacatg
      961 ctggatttga tcggggatga cgaagagaag aagaaagcca tcttctttga aaacgccaaa
     1021 cgcgtttact tcgattaccc gcagggaggc cagcaggcat gaataaagtt ttggcaaagc
     1081 accttggcaa catggatcag ctcgcgggcg tgcgccttag ccgcctggaa agcggaaacg
     1141 ccgcgggcaa gcggattgcc gaagtctaca acgccgcggg gctgcgcttt accgttctgc
     1201 cggataagtg catggacctg ttcgaccttt cgtataaggg cgtgaacatc gcgttccatt
     1261 caaagaacgc tctttcggcg ggcgaaccgt tctaccccgt tccgagcgag tttttcggct
     1321 actggtcggg cggcatgctc gccacctgcg ggcttgccaa catcggcggc gcggacgaaa
     1381 gcgaccccac gtgcgttcac ccgattcacg gtcgaatcgg ctaccagagc gcggactgct
     1441 ttggctatga ctgcgcctgg cagggcgagg attatatcct ctcgctggaa ggccgcatgc
     1501 gcgaaacgcg cctctacgga cgcagtctcg aactgcgccg ccgcatccgc aca
//
LOCUS       DAOMGY010000475         1545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_96580_length_1545_cov_2.895973, whole genome shotgun sequence.
ACCESSION   DAOMGY010000475 DAOMGY010000000
VERSION     DAOMGY010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96580_length_1545_cov_2.895973"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PLR57_07380"
     CDS             complement(<1..1052)
                     /locus_tag="PLR57_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HQQ41315.1"
                     /translation="MPESVQLRPTYSICPAGEMPREGRDRLSLEGFPLFAPERDGDFS
                     FSWDEYRLTFNPVNLLMQLTRDGLPLVCDRAPMGCNLGGEYGENMRHYLVREPGERIF
                     GLGDKGGKLDKAGRRFRLDSKDAMGYDAETSDPLYQHVPFYICQNSAGFAGIFYDTHA
                     SCAFDFGSEISNYTGPYRCAEIGENALVYYLLLGTLPEIVAGFSALTGGTAFLPRRSL
                     LYAGSTMTYTDAPDADAQLRAFADECGTRGFSCGGFYLSSGYTSIGQKRYVFHWNNEK
                     IPNPKALSAYFRERGIELIANIKPVFLTDHPLYETIANNGWFLRLADGTPALTPFWDG
                     LGSWLDFSNPGAYDFW"
     gene            1364..>1545
                     /locus_tag="PLR57_07385"
     CDS             1364..>1545
                     /locus_tag="PLR57_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41316.1"
                     /translation="MTYPIGSACLPRGFELNSLSVHNIRRVYRHHHDFYEREHAKIKD
                     NLLRWKNEEKVQKSEAG"
BASE COUNT          367 a          423 c          417 g          338 t
ORIGIN      
        1 ttccagaagt cgtacgcgcc gggattcgaa aaatccagcc agcttccaag tccgtcccag
       61 aatggcgtca gcgcaggcgt gccgtccgct aagcgcaaaa accagccgtt gttcgcaatc
      121 gtttcgtaga gcggatgatc ggttaaaaac accggcttga tattggcgat cagctcgatg
      181 ccgcgctcac ggaaataagc cgaaagcgct ttggggttcg gaatcttctc gttgttccag
      241 tgaaaaacat agcgtttctg cccgatggac gtgtacccgg acgaaagata gaatccgccg
      301 caggaaaagc cgcgcgtacc gcactcgtcc gcaaaagcgc ggagctgcgc gtccgcgtcc
      361 ggcgcgtcgg tataggtcat cgtactgccc gcgtagagaa gcgaacgccg cggcagaaac
      421 gccgttccgc ccgtcagcgc ggaaaacccc gcgacgattt ctggaagcgt accgagcagc
      481 aggtagtaaa cgagcgcgtt ctcgccgatc tcggcgcagc ggtatggtcc ggtgtagttg
      541 ctgatttcgc tgccgaaatc gaaggcgcag cttgcgtgcg tatcgtaaaa gattcccgca
      601 aaccccgcgc tgttttggca gatgtagaac ggcacgtgct gatacagcgg atcgctcgtt
      661 tccgcgtcgt aacccatcgc gtccttgctg tccagccgga accgccgtcc cgctttatcg
      721 agctttccgc ccttgtcgcc aagcccgaag atacgttcgc cgggctcgcg caccagatag
      781 tgccgcatat tttcgccgta ctcgccgccg aggttgcagc ccatcggggc gcggtcgcaa
      841 acgagcggca aaccgtctct tgtcagctgc atcagcagat tgaccggatt aaacgtaagg
      901 cgatattcgt cccaagagaa ggaaaaatct ccatcccgtt caggtgcaaa aagcggaaac
      961 ccttccagcg aaagcctgtc gcgcccttcc cgcggcatct ctcccgcggg acagatggag
     1021 taagtcggtc gaagctgtac gctttctggc aaaagggaga ttcttatgag cgctttgctt
     1081 aaaaaatcga cgcgtgaagt cagcccttcc gttttgtaaa ggctgaaata tgaggacgat
     1141 tcgatacagg aaaagagatg atcatactgc ggcaaatcaa aaaccacctt tgcaggatat
     1201 tctgattata cgttgcggcg ttcctccgcg ctattgcaaa agataccgtt tttattgaat
     1261 ttcgtgcgct taaggctatt atatttgctg tattttgcat gtttagcggt tcttgattga
     1321 aaaacgagcg attctctgtt acgatcaccc tatgagaact tacttgacgt atcccatcgg
     1381 cagcgcgtgc ctgccgcgcg gcttcgagct gaactcgctt tccgtgcaca acatccgccg
     1441 cgtgtaccgg catcaccatg atttttatga acgggagcat gctaaaataa aagacaatct
     1501 gctaagatgg aagaacgaag aaaaggttca gaaatcggag gcagg
//
LOCUS       DAOMGY010000476         1544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_96673_length_1544_cov_3.011417, whole genome shotgun sequence.
ACCESSION   DAOMGY010000476 DAOMGY010000000
VERSION     DAOMGY010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96673_length_1544_cov_3.011417"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PLR57_07390"
     CDS             <1..1077
                     /locus_tag="PLR57_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQQ41317.1"
                     /translation="NQARNFVTIASKQKQIERIKSTLVKPEEAPQGIHFKLRADALTA
                     NEVIVCRGLAKSYGGQTLFRNLDLLVRRDERVCLLGANGCGKTTLLKILTNRVEPDAG
                     SYKLGSNVHVGYYEQSTVHTSDTRTVLEALHSSFPRYDLQVFRNLLGSFLFRGDDVYK
                     HICDLSGGEYARIQLLRLMLSGSNVLFLDEPTNHLDIPSCEALEEALNEYGGTMLIVT
                     HDRYLANRIADRIILMDGDGVREFEGDWDAYKEFLAESAAPAEKEEPVVKNAYVLAKE
                     HKSAVNRCKGALDRAEKKVKEEEAKLGELETRVCAPEIASDYEAAKSLYEELETQRRL
                     VEACYAEWERAESELQSLLAEEEE"
     gene            1080..>1544
                     /locus_tag="PLR57_07395"
     CDS             1080..>1544
                     /locus_tag="PLR57_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41318.1"
                     /translation="MDYSKFTLGLRFAMTSDSALTPEDCWNLIFSEVPTTYTLEAHLF
                     GAWDDVARAESEAAYICMFSNLPLNTGKDFYVQLKKKPNLLSYLTIYRPFIQNNLVEK
                     CGKVEYLGEVQSDGTITGGDVSYGTMMFNGKEPEECKKNMKCPSVLIAPDSWE"
BASE COUNT          386 a          416 c          447 g          295 t
ORIGIN      
        1 aaccaggcgc gaaacttcgt taccatcgcc tccaagcaaa agcagatcga gcgcatcaaa
       61 agcacgctcg ttaagcccga agaagcgcca cagggcattc atttcaagct tcgcgcggac
      121 gcgctgacgg caaacgaagt gatcgtctgc cgggggcttg caaagagtta cggcgggcag
      181 acgctgtttc gcaatctgga tttgctggtg cgcagggacg agcgcgtatg cctgcttggc
      241 gcgaacggct gcggcaagac cacgctgctg aagattttga ccaatagagt cgagccggat
      301 gctggcagct acaaactcgg ttccaacgtg catgtcggtt actacgaaca gagcaccgtg
      361 catacgtccg acacgcgaac cgttctggaa gcgctgcatt catcgtttcc ccggtacgac
      421 ctgcaggtat ttcgcaatct gctcggctcg tttctgtttc gcggggacga tgtttacaag
      481 cacatctgcg acctctccgg cggcgagtac gcccgcattc agctgttgcg gctgatgctc
      541 tcgggcagta acgttctatt tctggacgag ccgaccaacc acctggacat cccttcctgc
      601 gaagcgctgg aagaagcgct caacgaatac ggcggaacga tgctgatcgt aacacacgac
      661 cgctaccttg ccaaccggat tgccgaccgc atcatcctga tggacggaga cggcgtgcgc
      721 gagtttgaag gcgactggga cgcgtataaa gagtttcttg ctgagagcgc ggctcccgcg
      781 gagaaggaag agcctgtcgt aaagaacgcc tacgttctgg caaaggaaca taaatccgca
      841 gtcaaccgct gcaaaggcgc gctcgaccga gcggaaaaga aggtcaaaga ggaagaagca
      901 aagctcggcg aactggaaac gcgcgtctgc gcgccggaaa tcgcgagcga ttatgaagca
      961 gcaaaatcgc tctacgaaga gcttgaaacg cagcgccgtc tggtggaagc ctgttatgcc
     1021 gaatgggaac gcgcggagtc ggaactgcag tcgctcttgg cggaggaaga ggagtaagaa
     1081 tggactattc aaagtttacg cttggactaa ggtttgccat gaccagcgat tccgcgctca
     1141 cgccggaaga ctgctggaat ctcatctttt ccgaagtgcc caccacatat acgctggaag
     1201 cacacctctt cggcgcgtgg gacgatgttg cgcgcgcgga atcggaagcc gcgtacattt
     1261 gcatgttttc caacctgccg ctcaacaccg gcaaggactt ttacgttcag ctcaagaaaa
     1321 agccgaacct gctttcgtac ctgacgatct accgtccgtt cattcagaac aacctcgtcg
     1381 aaaaatgcgg caaggtggag tatcttggcg aagtgcagtc ggacggaacc atcacaggcg
     1441 gagacgtttc atacggaacg atgatgttta acggcaaaga accggaagag tgcaaaaaga
     1501 acatgaagtg cccgagcgtt ttaattgcgc cggacagctg ggaa
//
LOCUS       DAOMGY010000477         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_97009_length_1540_cov_4.072054, whole genome shotgun sequence.
ACCESSION   DAOMGY010000477 DAOMGY010000000
VERSION     DAOMGY010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97009_length_1540_cov_4.072054"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLR57_07400"
     CDS             <1..553
                     /locus_tag="PLR57_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HQQ41319.1"
                     /translation="PRQIDDEAARNAIPPEKDSDGVCDRSLAGLFTDSKTGYAPCTAE
                     ACMALLEHEQIPLEGKRAVVVGRSLVIGKPVAMLLLQKNATVTIAHSRTKDLANVTCE
                     AEILIVAAGKRALIGAQHVRAGQIVLDVGMHFDEHGGPYGDVNFEAVEPVVSTITPAR
                     GGVGSVTTVILASHVVDAAERIF"
     gene            619..1191
                     /locus_tag="PLR57_07405"
     CDS             619..1191
                     /locus_tag="PLR57_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQQ41320.1"
                     /translation="MNVCILMGSARKNGNTAELMKPFRDELEKNGAEISYFALAEMDV
                     HPCKGCYHCQDVQEGYGCNQKDDMPRIAEAIIKSDLIVLATPIYTWYCTAELKAVLDR
                     FYGLAAMYRSARRNLVEGKRVCILATHGYDASYAADPFVIGIQRMCEHYGMRYNGMYS
                     VRDADDLASFQTKQAQSGAREFAQYLLSLR"
     gene            1230..>1540
                     /locus_tag="PLR57_07410"
     CDS             1230..>1540
                     /locus_tag="PLR57_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQQ41321.1"
                     /translation="MAAPKNDNVKQQILDAAIELFQEQHDVSLAEIAKASHVSKGTLF
                     YHYRSKAEIYLDIGERYWSKLSNDLLIWVDNKEKDTSIPRLVRYTFIRGVFDESGLLR
                     L"
BASE COUNT          371 a          409 c          434 g          326 t
ORIGIN      
        1 cccaaggcaa atcgacgacg aagccgcgcg caacgctatc cctccggaaa aggattccga
       61 cggcgtctgc gaccggtcgc ttgcgggact gttcaccgac tcaaagaccg gttatgcgcc
      121 ctgcacggcg gaagcctgta tggcgcttct tgagcacgag caaatcccgc tcgaaggcaa
      181 aagagccgtc gtagtaggcc gcagcctcgt aatcggcaaa cccgtagcca tgctgctgct
      241 gcagaaaaat gcgaccgtta cgattgcgca ttcgcgcaca aaggatttgg cgaacgttac
      301 gtgcgaagcg gaaattctca tcgttgccgc gggcaagcgc gctttaatcg gcgcgcagca
      361 tgttcgcgct ggtcagattg tactggacgt cggcatgcat tttgacgagc acggcggccc
      421 atatggcgac gtgaatttcg aagccgtgga acctgtcgta tccacaatta cgccagcgcg
      481 tggcggagtc ggttccgtta ccaccgtcat cctcgcaagc cacgttgttg atgcggcaga
      541 acgaattttt taggtctgaa tcgcctttta tcgcaaatca ccttttgtaa aagaataaaa
      601 gatcgagagg aaacacgtat gaacgtctgc atcctcatgg gcagcgcccg caaaaacggc
      661 aacacggcgg aactgatgaa acccttccgc gatgagcttg aaaagaacgg cgcggagatt
      721 tcttattttg ctttggcgga gatggacgtc catccctgca aaggctgcta tcattgtcag
      781 gatgtgcagg aaggctacgg ctgcaaccag aaggacgata tgccgcgcat cgcggaggct
      841 atcataaaaa gcgatttaat cgtgcttgcc acccccatct acacctggta ctgcacggcg
      901 gaactcaaag cggttctcga ccggttctac gggttggcgg cgatgtatcg ctcagcgcgc
      961 agaaatctcg tggagggaaa gcgcgtttgc attctcgcga cgcacggcta cgacgcttcc
     1021 tacgccgcgg atccttttgt aatcggcatt cagcggatgt gcgaacacta cggaatgcgg
     1081 tataatggaa tgtattccgt gcgggacgcg gacgatttgg cttcgtttca gactaagcag
     1141 gcgcagtccg gcgcgcggga gtttgcgcag tatctgctct ctctgcgcta aaaagcgggt
     1201 tcgtgacatc cggaaagggg taatgctcta tggcggctcc aaaaaacgat aacgtaaaac
     1261 agcagattct cgacgcggca atcgagctgt ttcaggagca gcacgacgtt tctctcgcgg
     1321 agattgcaaa agcctcgcat gtgagcaaag gcacgctgtt ttaccactac aggagcaaag
     1381 cggaaatcta cctcgatatc ggcgagcggt actggagcaa actttccaac gaccttttaa
     1441 tctgggtgga taataaagaa aaggacacct ctattccccg ccttgtaagg tacacgttca
     1501 ttcgcggcgt gtttgacgaa agcggactgc tgcgcctgag
//
LOCUS       DAOMGY010000478         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_97041_length_1540_cov_2.945455, whole genome shotgun sequence.
ACCESSION   DAOMGY010000478 DAOMGY010000000
VERSION     DAOMGY010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97041_length_1540_cov_2.945455"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLR57_07415"
     CDS             <1..457
                     /locus_tag="PLR57_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006907109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HQQ41322.1"
                     /translation="ANREKYPKSYAEKLLYLEETQYSPMHFHWSKMEDIINRGGGNVL
                     IRVYNSTPDERFDTTDVTLHCDGRVYTVPAGTQVRLTPGESISIYPYMYHDFTVEAGT
                     GPVLLGEVSMVNDDVHDNRFYEPLGRFSTIEEDEAPYRLLCNEYPAAKD"
     gene            464..1291
                     /locus_tag="PLR57_07420"
     CDS             464..1291
                     /locus_tag="PLR57_07420"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQQ41323.1"
                     /translation="MTKIRLCALDVDGTLIDSSLAITEGVARAVDALEQAGIIPAVVT
                     GRTVKELEVLRARFPYLRYFIVSNGAYAFDSVTGACFYKDLLPLELAKTVVQEAQNYS
                     VMIDIYADGASYVDAAAWANKAAYRVEFLTHPSLGPARIPTEDVRRFLADRTSDVEKL
                     YISFEHLADLEKLHAFCQKLPVDLVVSIHDGLEVNQRGVEKGSGLKALCGLLGILQEE
                     TAAIGDGFADIPMLQCAGLPIAMGNAAEEVKAAAKLVAPDHDHDGAAWAIEQILAAQ"
BASE COUNT          351 a          455 c          432 g          302 t
ORIGIN      
        1 tgccaaccgg gaaaaatacc cgaaatccta cgccgaaaaa ctgctctacc tcgaggaaac
       61 gcaatattcg cccatgcact tccactggag caaaatggag gatatcatca accgcggcgg
      121 cggcaacgtg ctcatccgcg tgtacaactc cacgccggac gaacgcttcg atacgacgga
      181 cgtgacgctc cactgcgacg ggcgtgttta cacggtgccc gcaggcacgc aggtgcgcct
      241 gacccccggc gagagcattt cgatttatcc ctatatgtac cacgatttca ccgtggaagc
      301 gggcacgggc cccgttctgc tcggcgaagt cagcatggtc aacgacgacg tgcacgacaa
      361 ccgattctac gagcctctcg ggcgtttttc caccattgaa gaggacgaag cgccctaccg
      421 tctgctctgc aacgaatacc ccgcagcaaa ggactgagcg cgcatgacaa agattcgcct
      481 ttgcgcgctg gatgtagacg gcacgctgat cgacagcagt ctggcgatta ccgagggagt
      541 tgcccgcgcg gtggacgcgc tcgagcaggc cggaatcata cccgccgtcg tgaccggcag
      601 aacggtcaag gaactggaag tgcttcgcgc gcggtttccg tatttacgat acttcatcgt
      661 cagcaacggc gcgtacgcgt tcgattccgt gaccggggcg tgtttctaca aagatctgct
      721 tccgctggaa ctcgcgaaaa ccgtcgttca ggaagcgcaa aattattcgg taatgattga
      781 tatatacgcc gacggagcat cctatgtgga cgccgcagcc tgggcgaaca aagccgccta
      841 ccgcgtggaa tttttaacgc acccctccct cggccctgcg cgcatcccaa ccgaagacgt
      901 tcggcggttt ttggccgatc ggacgagcga tgttgaaaag ctctacatct cgtttgagca
      961 tttggcagac cttgaaaaac tccatgcgtt ctgccaaaag ctcccggtcg atctggtcgt
     1021 ctccatccat gacggattgg aagtcaacca gcgcggcgtg gaaaagggct ccgggctcaa
     1081 agcgctgtgc ggccttctcg gcattctgca ggaggaaacg gctgccatcg gcgacgggtt
     1141 tgcggatatc ccgatgctcc agtgcgccgg gcttcccatc gcaatgggca acgcggcgga
     1201 ggaagtcaag gcggcggcaa agctcgttgc gcccgatcac gatcacgacg gcgcggcctg
     1261 ggcaattgaa caaatactcg ctgcgcaata gctttaatcg agcaatttta aatactgcat
     1321 tcaggtgctc gccgcgcaat agcttttatc gagcaatcaa aatatcgcac gcaggtgctc
     1381 gccgcgcgct aacgcggaca atcgaatcag caagctatca tacacacata cacaaacgaa
     1441 cggagggttt tgtctatgca gcggaatcgt ttggtctttg tcggcagtta tacgcagtcc
     1501 atcgtgctcg gcaccgggga aaccgttccc ggcagcggcg
//
LOCUS       DAOMGY010000479         1539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_97180_length_1539_cov_1.657008, whole genome shotgun sequence.
ACCESSION   DAOMGY010000479 DAOMGY010000000
VERSION     DAOMGY010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97180_length_1539_cov_1.657008"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..979)
                     /locus_tag="PLR57_07425"
     CDS             complement(182..979)
                     /locus_tag="PLR57_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQQ41324.1"
                     /translation="MAFIEVERLIVSSSWKMQNPHKHSHYEIYYLQEGMREHIIDGRL
                     YYAASHSFVLIPPNTPHQTEGTPFNRLNINFDADYLAVPPQDLLTRCFESTMIPIAAE
                     DLPVFARLLEQIEQEYAYSIGKNDQLLPLLVGELIVLLDRYARDHAVSASASFEGGAV
                     FSKILRYVDEHFLTCTPEEAAKAFFLSAAHLSRQFHRYTGMTFSKYVLNKKIAKAMYL
                     LKHTQLDMGAVAEKCGFLSANYFSMRFKKVVNLSPLQYRKAHDVKRI"
     gene            1106..>1539
                     /locus_tag="PLR57_07430"
     CDS             1106..>1539
                     /locus_tag="PLR57_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQQ41325.1"
                     /translation="MKKLSMGVKLSYGLGDFYGGASAAIIGLFFLFFLTNVVGISPLV
                     AGSIVLFGRAVDAVTDPLMGSISDHTRSKWGRRVAWFFFGILPVALSYVLMWVVPPFE
                     RWLQVAYYFFAYAFFSCAFTMVMVPYGALPQDLTVDANERTVL"
BASE COUNT          311 a          396 c          453 g          379 t
ORIGIN      
        1 actgtgaatt atctggttgg aaagaatgac gagatgagct ccgttccggc tttgtttacg
       61 gaggttcttg cactgatgcg ggagggcttt ttgcagctcg gcgacggttg agattccatg
      121 ctcgggcaaa gcggttcggc aggccggaat ccggcaggaa cgaacaagcg caagcctaaa
      181 actatatccg ctttacgtcg tgcgccttgc gatactggag cggggaaaga ttgacaactt
      241 ttttaaagcg catgctgaag tagttggccg aaaggaagcc gcatttttcc gcgacagcgc
      301 ccatgtccag ctgcgtatgc ttgagcaggt acatcgcttt tgcaatcttc ttgttcagaa
      361 cgtatttact gaacgtcatg ccggtatagc ggtggaactg acggctgagg tgcgcggcgg
      421 agaggaagaa tgctttggcg gcttcctccg gcgtgcaggt taaaaagtgc tcgtccacat
      481 accggaggat tttggaaaac accgcgccgc cttcgaacga cgcgctggcg gaaacggcgt
      541 gatcccgcgc gtaacgatct aaaaggacga tcagttcgcc gaccaaaagc ggaaggagct
      601 ggtcgttttt tccaatgctg taagcgtact cctgttcaat ctgctccagc aaacgggcaa
      661 acaccggcaa gtcttccgcc gctatcggaa tcatcgtgct ttcaaagcag cgcgtcagca
      721 agtcctgcgg cggaacggcg agatagtcgg cgtcgaagtt gatgttcagg cggttgaagg
      781 gcgtgccttc cgtttggtgc ggcgtgttcg gcggaatcag cacaaagctg tggctcgcgg
      841 cgtagtagag ccgaccgtcg atgatatgct cgcgcatgcc ctcctgcagg tagtagattt
      901 cgtaatggct gtgcttatgc ggattctgca ttttccaact gctggaaacg atcagccgct
      961 ccacctcgat aaacgccata cgccctcctg cctgcgagat caaaatatat gtaattatag
     1021 catatttaat catattgtga cagggtaccg ccaaaacggc tatgctatac tgagcctatc
     1081 aaacagtggg aaggtatggg gtagtatgaa gaaactcagt atgggcgtca agctttctta
     1141 tggcctcggg gacttctacg gcggcgcttc cgccgcgatt atcggcctgt tttttctttt
     1201 tttcctgacg aacgttgtcg gcatttcgcc gctggtcgcg gggtcgatcg tgctctttgg
     1261 ccgcgcggtg gacgcggtaa ccgacccgct gatgggctcc atcagcgacc acacccgctc
     1321 aaagtgggga cggcgcgtcg cctggttctt ctttggcatt ctgccggttg cgctgtccta
     1381 cgtgctcatg tgggtcgtgc cgccgtttga acgttggctc caggttgcgt attacttttt
     1441 tgcctacgcg ttcttctcct gcgcgttcac catggttatg gtgccgtatg gcgcgctgcc
     1501 gcaggacttg accgtagacg cgaacgagcg cacggtgct
//
LOCUS       DAOMGY010000480         1534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_97592_length_1534_cov_3.505071, whole genome shotgun sequence.
ACCESSION   DAOMGY010000480 DAOMGY010000000
VERSION     DAOMGY010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97592_length_1534_cov_3.505071"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="PLR57_07435"
     CDS             complement(<1..1422)
                     /locus_tag="PLR57_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HQQ41326.1"
                     /translation="MKKSSVRILSVLFTLALAFSLCALPGCAKNGTSGGPELSAQLIT
                     TQAPSASPEPTPTISPVPVPQLKYIFLFIGDGMSTPQIQAASEALAAVNRAPLSFVDF
                     PVCGFAHTKSVTGETTDSAAAATAISSGVKTVNGYLGLDPNGNRVTSIAELLRDMGRK
                     IGILTTVSLDHATPAGFYAHVVSRRTYTDIADDLFASGFDFFAGGGFHDTPDAAEHAA
                     ENGYTLVSSPSDAPAGASEKLIVSSNLIFGDYGVLPAIDGGARMHFLRDVTSLAISRL
                     TSPQGFYMMVEGGRIDYFCHYNDAGSFVAELLDFDEAVAAAISFYQAHPYETLILVTG
                     DHETGKVSFTDGDRTALLKQTISCDECDDTLAADCIATQTPFETALPLFAEAFGLDNL
                     TAEETEYLKDAYTHTLKGDLPSNKVYEEYGVYEPITSACARLVAERAGLVFGSGSHSD
                     LDVPVFALGVGQELFSGDYENTFL"
BASE COUNT          334 a          423 c          476 g          301 t
ORIGIN      
        1 cagaaaggtg ttttcatagt ctccgctgaa gagctcctgt ccgacgccga gagcgaaaac
       61 gggaacgtcc agatcgctgt gtgagcccga accgaacacc agtcccgcgc gctcggcaac
      121 gagtcttgcg caggcggagg tgatgggctc gtacacgccg tattcttcgt aaaccttatt
      181 gctgggcaga tcgcccttga gcgtgtgcgt ataagcgtcc ttcaggtact ccgtttcttc
      241 cgctgtaagg ttatccagcc cgaacgcttc cgcaaagagc ggcagggcgg tttcaaacgg
      301 cgtttgagtg gcaatgcagt cggcagccag cgtatcgtca cactcgtcgc aggagatcgt
      361 ctgtttgagc agcgccgtcc tgtcaccgtc cgtaaacgaa acttttccgg tttcgtggtc
      421 gcctgtgacc agaattagcg tttcatacgg gtgcgcctga taaaacgaaa tggcggcggc
      481 aaccgcctcg tcaaagtcga gcagttcggc aacgaacgag cccgcgtcgt tataatggca
      541 gaaataatcg attcgtccgc cttccaccat catgtagaat ccctgcggcg aggtcaggcg
      601 gctgatggcg agggacgtaa cgtcccgcaa gaagtgcatc cgcgctccgc cgtcaatggc
      661 gggcagcacg ccgtaatcgc caaagatgag attggaagag acgatgagct tctccgaagc
      721 gccggcgggc gcatccgaag gggaagaaac aagcgtatag ccattttcgg ctgcgtgttc
      781 cgccgcatcc ggcgtatcgt gaaagccgcc gcccgcaaaa aagtcgaacc cgctggcgaa
      841 caggtcgtcc gcgatgtcgg tatacgttct gcgggaaacc acatgcgcgt agaatccggc
      901 gggcgtcgcg tgatccagag agaccgtggt gaggattccg attttgcgcc ccatatcgcg
      961 cagcagttcg gctatgctgg ttacgcggtt gccgttcggg tcgagaccga ggtagccgtt
     1021 gaccgttttg accccgcttg aaatcgcggt cgcggcggcg gcggaatccg tcgtttcgcc
     1081 ggtaacgctt ttcgtgtgcg caaagccgca aacgggaaag tccacgaacg aaagcggcgc
     1141 gcggtttaca gcagccagcg cttcggacgc ggcctgaatc tgcggcgtgc tcatgccgtc
     1201 tccaataaag aggaagatat atttcaactg cggaacaggt acgggtgaaa tggtgggggt
     1261 tggctcgggc gaagcggaag gcgcttgtgt cgtaatcagc tgcgcgctga gctccgggcc
     1321 gccggaggtt ccgttttttg cgcagccggg cagagcgcag agcgagaatg ccagcgcgag
     1381 tgtgaaaaga accgatagaa ttcgaacaga cgattttttc atgcgcatac gtcattctag
     1441 cacgatttga tcgcccgtac aactggacgc ggacgcgcgc gaatggtaag atgaacgaaa
     1501 cagggcattt caccgcggcg caaaaacatc gccc
//
LOCUS       DAOMGY010000481         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_97998_length_1530_cov_2.970847, whole genome shotgun sequence.
ACCESSION   DAOMGY010000481 DAOMGY010000000
VERSION     DAOMGY010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97998_length_1530_cov_2.970847"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /gene="tdh"
                     /locus_tag="PLR57_07440"
     CDS             complement(<1..1035)
                     /gene="tdh"
                     /locus_tag="PLR57_07440"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HQQ41327.1"
                     /translation="MIPAYMHALVKETAGKGLTLKQVKTPVPGIGEVLIKIHKTAICG
                     TDVHIYNWDAWSQKTIVPPMTIGHEYVGEIAVLGPGVEDYHVGQIVTGEGHIVCGHCR
                     NCRAGNGHWCKFTKGVGVNRDGAFAEYLCIPETNVIAVPETTPEEIVAFYDAYGNACH
                     TATMFDVTGEDVLITGAGPIGMMAVGICRQNGARRIVVTDVNDYRLEIAKKMGASATV
                     NLKTQFVEDAMKSLHIVEGFDVGCEMSGNGNALNQMLKLMRNGGKVALLGIAGPGTVI
                     DWNDVIFKGLTLQGIYGRKMYETWYKMMAMVEAGLDLSPVLTHRYHYSDFEEAFAVMN
                     SGQSGKVVLDW"
     gene            complement(1231..>1530)
                     /locus_tag="PLR57_07445"
     CDS             complement(1231..>1530)
                     /locus_tag="PLR57_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HQQ41328.1"
                     /translation="IAERLEENNIVVNYQATPEEEGFTASAALRLGVSEMTRFGFGKA
                     EFEQLAHLMADCVLRNKPVAQEVAKLRSNYTKLGYCFNDAEVTQALDALSAQSGM"
BASE COUNT          277 a          482 c          402 g          369 t
ORIGIN      
        1 ccagtccaga acgaccttgc cgctctggcc gctgttcatc acggcgaacg cttcctcaaa
       61 gtccgaataa tgatagcggt gcgtcagcac cggggaaaga tccagccccg cttccaccat
      121 ggccatcatc ttgtaccacg tttcatacat cttgcggcca tagattccct gcagtgtcag
      181 acctttaaag ataacatcgt tccagtcgat caccgtaccc ggccccgcga tgccaagcag
      241 cgccacttta ccgccgtttc gcatgagctt gagcatctgg ttaagcgcat tcccgttgcc
      301 gctcatttcg cagccgacgt caaagccttc tacaatatgc aggctcttca tcgcatcctc
      361 gacgaactgg gttttcaggt tgacggttgc gctcgccccc atctttttgg caatctcgag
      421 acggtagtcg ttcacgtccg tcaccacgat gcgccttgcc ccgttctggc ggcagatgcc
      481 gaccgccatc atgccgatgg gccccgcacc cgtgatgagc acgtcctcgc cggtcacgtc
      541 gaacatggtc gccgtatggc aggcgttgcc atacgcgtcg taaaacgcga cgatctcctc
      601 cggcgtggtc tccggcacgg cgatgacgtt cgtttccgga atgcataggt attcggcaaa
      661 cgcgccgtcg cggttgacac ccacgccctt ggtaaacttg caccagtgtc cgttgcccgc
      721 gcggcagttg cggcagtgcc cgcagacgat atggccttcg ccggtcacga tctgtccgac
      781 gtggtagtcc tccacgccgg ggcccagcac cgcgatttcg ccgacgtact cgtggccgat
      841 cgtcatggga ggaacaatcg ttttctggct ccaggcgtcc cagttgtaga tatgaacgtc
      901 cgtgccgcag atggcggttt tgtggatctt aatgagcact tccccgatgc ccggcacagg
      961 cgttttgacc tgcttgagcg tcagcccttt tccagcggtt tccttgacca gcgcgtgcat
     1021 ataggccgga atcataatcg atcagccctc cgtttgaaga ttctttttca aatgaacact
     1081 gttagacttt tttgttgctc tctttatact gtagtgcgtc ctcgctccgt tttcaacgct
     1141 ttggcaacaa tataaaatcc tcgcgcccgg cgaaaacccg cccgccggaa attctgttcc
     1201 ggcgggcggc tttctatgat ttatatgtgc ttacataccg ctctgcgcgc tgagcgcgtc
     1261 aagggcttgg gtcacttccg cgtcgttaaa gcagtagccg agtttcgtgt agttggaacg
     1321 cagcttcgcg acttcctgcg caacgggttt atttcgcagc acgcagtccg ccatcaggtg
     1381 cgccagttgt tcgaactccg ccttgccaaa gccgaagcgc gtcatttcgg aaacgccgag
     1441 gcgcagcgcg gcgcttgcgg taaagccctc ttcctcgggc gtcgcctgat agttgacgac
     1501 gatgttgttc tcctccagcc gctcggcgat
//
LOCUS       DAOMGY010000482         1528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_98214_length_1528_cov_2.564155, whole genome shotgun sequence.
ACCESSION   DAOMGY010000482 DAOMGY010000000
VERSION     DAOMGY010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98214_length_1528_cov_2.564155"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="PLR57_07450"
     CDS             complement(<1..1138)
                     /locus_tag="PLR57_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQQ41329.1"
                     /translation="MTQDELILEFSKKYTPEQTEVFKKAVAFAANVHASQLRESGELY
                     IVHPIEVSRMVMEMGMDAASVTAAMLHDVVEDGDNVTVADLQREFGDEIANLVDGVTK
                     LTKSGQQTYITKKQEQSENLRKLFLAIAADVRVVIIKLADRLHNMRTLAYCNPYKRTR
                     KAKETLEVYAPLAHRFGMGAIKGEMEDLCFMYLMPEEYASISRQFKEKQTERQETLDE
                     AKRIIGETLKEAGIEASISGRPKHLYSVYKKMQRLNSGINEVYDLIAIRVIVETVNDC
                     YAALGVIHANWKPLPGRFKDYIATPKPNMYRSLHTTLLAPNGIPFEVQIRTKEMHRTA
                     EYGIAAHWMYKEGRSVQSLLDKKTSWLRQVIEAKENTEDSKEFVE"
     gene            complement(1232..>1528)
                     /locus_tag="PLR57_07455"
     CDS             complement(1232..>1528)
                     /locus_tag="PLR57_07455"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0106130 - purine phosphoribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine phosphoribosyltransferase family protein"
                     /protein_id="HQQ41330.1"
                     /translation="FVLARKPGKLPCETLKYEYGLEYGSDVLEIHKDAIKPGQKVVIV
                     DDLLATGGTALATIKLVEQAGGEVVGVRFAIELCSEFDGRAPLKGYDVQSVMQY"
BASE COUNT          284 a          427 c          422 g          395 t
ORIGIN      
        1 tctcgacgaa ttccttgctg tcctccgtgt tctcctttgc ctcgatgacc tgtctaagcc
       61 agctcgtctt tttatcgagc aggctctgca cggagcggcc ttccttatac atccagtgcg
      121 cggcgatgcc gtattccgcc gtgcggtgca tttccttggt acgaatctgt acttcaaagg
      181 gaatcccgtt cggcgcaagc agcgtcgtat ggagcgagcg gtacatattt ggtttcgggg
      241 tcgcgatata gtccttaaag cgtcccggaa gcggcttcca gttcgcgtgg atcacgccaa
      301 gcgccgcgta gcagtcgtta acggtttcca cgattacgcg gatggcgatg aggtcgtaca
      361 cttcgttgat gccggaattg agccgctgca tctttttata cacgctgtag agatgcttcg
      421 gtcgcccgct gatggaagcc tcgatgcccg cttccttgag cgtttcgccg atgatgcgct
      481 ttgcttcgtc cagcgtttcc tgccgctcgg tctgtttttc cttaaactgg cggctgatgc
      541 tggcatattc ctcgggcatc aggtacataa aacagaggtc ttccatttcg cctttaatgg
      601 cgcccatgcc gaagcggtgt gccagcggcg cgtagacctc cagggtttcc tttgcttttc
      661 gcgtgcgctt gtaggggttg cagtaggcca gcgtccgcat gttgtgcagg cggtcggcga
      721 gcttgataat gaccacgcgc acgtccgccg cgatggctaa aaagagcttc ctaaggtttt
      781 cggactgctc ctgctttttc gtgatatacg tctgctggcc ggacttggtc agcttggtaa
      841 cgccgtcaac gaggttggcg atttcgtccc cgaattcgcg ctgcaggtcg gcaacggtga
      901 cgttgtcccc gtcctcaacc acgtcgtgga gcatggcggc ggtaacgctc gcggcgtcca
      961 tgcccatctc catgaccatg cggcttactt cgatcgggtg cacgatataa agttcgccgg
     1021 attcgcgcag ctgggaagcg tgaacattcg cggcaaaagc gacggccttt ttaaagacct
     1081 ccgtctgttc cggcgtatac tttttggaga attccagtat cagctcgtcc tgtgtcatat
     1141 cggcacctcc tctccggcaa aatcgttcaa ttgctgcggg tgtagaaaaa atcaacggcg
     1201 aaccgttgat ttttcgctga acaggttgta attaatactg catgacggac tgcacgtcgt
     1261 agcctttcag cggagcgcga ccgtcgaatt ccgaacacag ttcaatcgca aatcgaacgc
     1321 cgacaacttc gccgcccgcc tgttcgacga gcttaatggt tgcaagcgcg gtgccgccgg
     1381 ttgcaagcag gtcgtccaca ataacgacct tctggccggg tttgatcgcg tctttatgaa
     1441 tctccagcac gtccgagccg tactcaaggc cgtattcata cttgagcgtt tcgcagggaa
     1501 gtttgccggg ttttctggca agaacaaa
//
LOCUS       DAOMGY010000483         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_98285_length_1527_cov_3.603940, whole genome shotgun sequence.
ACCESSION   DAOMGY010000483 DAOMGY010000000
VERSION     DAOMGY010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98285_length_1527_cov_3.603940"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLR57_07460"
     CDS             complement(<1..1004)
                     /locus_tag="PLR57_07460"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQQ41331.1"
                     /translation="MNAGFDLDQYLSDGVETIVSDALKASLKNPKETAFLLQYALHAK
                     RAEAARKNHAKAGRHVPSFLIASITSRCNLHCAGCYARANSICHDGADDNLLSDEQWD
                     SIFVQAKEIGVSFVLLAGGEPMLRRGVMERASSHPEILFPIFTNGTLLGEKTLDLLDS
                     HRNLIPIISVEGSQEQTDARRGAGTYQKLLETMTALQSRGVFFGASVTVTSENLAFVT
                     SDAFLNDLRTRGCKIAFYVEYVPADSGTESLAPGDSERVYLTSRLDELRARPDAMVYV
                     AFPGDEQYAGGCMAAGRGFFHINPHGGAEPCPFSPVSDTSLKDVSLLEALDSPLFVRL
                     R"
     gene            1271..>1527
                     /locus_tag="PLR57_07465"
     CDS             1271..>1527
                     /locus_tag="PLR57_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQQ41332.1"
                     /translation="MQKHKKEEIVRVVKYVLFSASAGVIQFVTFTLLNEIVQWPYWPC
                     YLIALVLSVLWNFTLNREFTFKSAANIPRAMLLVAAYYAVFS"
BASE COUNT          322 a          431 c          442 g          332 t
ORIGIN      
        1 cgcaagcgca caaacagcgg cgaatcaagc gcttccagca gggaaacgtc cttcaggctc
       61 gtatccgaaa ccggcgaaaa cgggcagggc tccgcgccgc cgtgcgggtt gatatggaag
      121 aaaccgcgcc cagccgccat gcaaccaccc gcgtactgct cgtcgccggg gaaggcgacg
      181 tagaccatcg catcaggccg cgcacggagc tcgtccagcc ggctggtcag gtagacgcgc
      241 tcgctgtctc cgggtgcgag agattccgtg ccggaatcgg cgggaacgta ttccacgtag
      301 aacgcaatct tgcacccgcg cgtgcgaagg tcgttcagga acgcgtcgct cgtgacaaac
      361 gcgaggtttt cgcttgtgac ggtgacggaa gcgccaaaga acacgccgcg gctttgaagc
      421 gcggtcatgg tttccaaaag cttttgatag gtgcccgcgc cgcgccgcgc gtcggtctgc
      481 tcttggctgc cttccacgct gatgatggga atcaggttgc gatggctgtc gagcagatcc
      541 agcgtttttt cgccgagcag cgtgccgttt gtgaaaatgg gaaacagaat ctcggggtgg
      601 gaactcgcgc gctccattac gccgcgccgc agcatcggtt cgccgcccgc gagcagaacg
      661 aacgaaacgc cgatctcctt tgcctgcacg aaaatggagt cccactgttc gtcgctgagc
      721 aggttgtcgt ccgcgccgtc gtggcaaatg ctgttggcgc gcgcgtaaca gcccgcgcag
      781 tgcaggttgc agcggctcgt gatgctggcg atgaggaagg agggcacatg ccgccccgct
      841 ttggcgtggt ttttacgcgc cgcctccgcg cgcttggcat ggagcgcata ctgcagcaaa
      901 aacgccgttt ccttaggatt tttcagcgaa gctttcaacg cgtccgaaac aatggtttcc
      961 acgccgtcgc tgaggtattg gtcgagatca aatcccgcgt tcatgccgct tgcctccgtt
     1021 gacttcttgt caaaattaga ttctttgtcc ataacgaccg catactatca gaatcgcgcg
     1081 caaaacacaa gagccggacg gttgagaggg gtaaaatctg tgtaaaacat ccgtcgagcg
     1141 cgttttccgt attgacgcgg ctggaaaatc gtgctatatt cgtttctgta ctaaagtcca
     1201 aattgaaatt caaaattact tatctttcat tcgcctgatc caatcctcaa aacagagaag
     1261 gagcgcaatc atgcaaaagc ataagaaaga agaaattgta cgggtcgtca agtacgtgct
     1321 gttttccgcg tccgcgggag tgattcagtt cgtcaccttt acgctgctca acgaaatcgt
     1381 ccagtggccg tattggccct gctacctgat tgcgctggtg ctgagcgtgc tttggaactt
     1441 tacgctcaac cgcgagttta cctttaagag cgcggccaat attccgcgcg ccatgctgct
     1501 ggttgcggcg tactacgcgg tgttttc
//
LOCUS       DAOMGY010000484         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_98447_length_1526_cov_2.456832, whole genome shotgun sequence.
ACCESSION   DAOMGY010000484 DAOMGY010000000
VERSION     DAOMGY010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98447_length_1526_cov_2.456832"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PLR57_07470"
     CDS             complement(<1..162)
                     /locus_tag="PLR57_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylglyoxylate dehydrogenase"
                     /protein_id="HQQ41333.1"
                     /translation="MSEVQVLNGNKAAAIAAALCRPGIIAAYPITPQTPLVEYLADFA
                     ADGVIDSTVS"
     gene            complement(166..435)
                     /locus_tag="PLR57_07475"
     CDS             complement(166..435)
                     /locus_tag="PLR57_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQQ41334.1"
                     /translation="MEFVSDYIVPIGCEGIHYIQTGKWRTRRPVLNAETCIKCGVCLM
                     YCPVNSVLRDADGSFRINYDFCKGCGICAHECKQKAITMVAEKEE"
     gene            complement(488..1036)
                     /locus_tag="PLR57_07480"
     CDS             complement(488..1036)
                     /locus_tag="PLR57_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQQ41335.1"
                     /translation="MKEIRLHGIGGLGTVKAGEILVHAAVLDGKFGNSTPFFGFERQG
                     APVTSFVRLDDRKIRPKNQIYHPNCAIVIDPSLLVSAPVFEGLAPESFFILNTKAGDV
                     SKLAEHENIRTVAYLNATQIALDELGRAIPNTVMLGAFVRATGWVRKDLMEQKVRESF
                     GEKNVTAFHRGYEEVQIQQFDR"
     gene            complement(1109..>1526)
                     /locus_tag="PLR57_07485"
     CDS             complement(1109..>1526)
                     /locus_tag="PLR57_07485"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQQ41336.1"
                     /translation="LREKGDPNNVWKAFPGYPALETHLPALLSEGCIKRGLDIERLVC
                     AVTKRPAELLGLYPRKGTLLPNSDADVVVVDLQTLKTVRADSLHSRSDFSIHEGRAFS
                     GWPSLTIKNGVIIARDGQPTGQRAAVRVITRKTGSY"
BASE COUNT          285 a          427 c          466 g          348 t
ORIGIN      
        1 gctcaccgtg ctgtcgatta cgccgtccgc cgcaaagtcg gcaaggtatt ccacaagcgg
       61 cgtctgcggg gtgatggggt acgcggcaat gatgccgggg cggcagagtg cggcggcgat
      121 agcggcggct ttattgccgt tgagaacctg tacttcgctc atgcctcact cctccttttc
      181 cgcgaccatt gtaatggcct tctgtttgca ttcgtgcgcg cagatgccgc agcccttgca
      241 gaaatcgtag ttgatgcgaa agcttccgtc cgcatcgcgc aaaaccgagt tgacgggaca
      301 gtacatcagg cagacgccgc atttaatgca cgtttcggcg ttcagaacgg gccttctggt
      361 gcgccatttt cccgtttgga tataatgaat cccttcgcag ccgatgggaa cgatatagtc
      421 gctgacaaat tccattttgg agctctccaa tccgtctagt atgtattgcg gttcaatcaa
      481 agcgcgttca gcgatcgaac tgctgaatct gcacttcttc gtaaccgcgg tggaaggcgg
      541 ttacgttctt ttcaccaaag gattcccgca ctttctgttc catcaagtcc ttgcgtaccc
      601 agccggtggc gcggacaaac gcgccgagca taacggtgtt cggaatcgcg cgacccagct
      661 cgtccagcgc gatctgcgta gcgttgagat acgccacggt gcggatgttt tcgtgctcgg
      721 cgagctttga aacgtcgcct gccttcgtgt tgagaatgaa aaaactctcc ggcgcaagtc
      781 cttcgaacac gggcgcggaa acgagaaggc tggggtcgat cacgatggcg cagttcgggt
      841 ggtagatctg gttcttgggt cgaattttgc ggtcgtccag acggacgaac gacgttacgg
      901 gcgcgccctg ccgttcaaag ccgaagaacg gcgtggagtt gccgaatttt ccgtccagca
      961 ccgcggcgtg gacaaggatt tctccggctt ttacggtacc gaggcctcca attccgtgga
     1021 gcctgatttc tttcatagcg gtgtttcctc cgttagattc aatagccgcc gtttctgcgc
     1081 ccgacgcgct cctgcggctg acgatgactt aataactgcc ggttttgcgc gtgataaccc
     1141 gcacggcggc gcgctgtccg gtgggctgac cgtcccgggc gatgatcacg ccgtttttaa
     1201 tcgtaagcga cggccagcct gagaaggcgc ggccttcgtg gatagagaag tcggagcggg
     1261 agtgcagcga gtctgcgcgc accgttttga gcgtctgcag atcgaccacg acaacatccg
     1321 cgtcgctgtt gggtaacagc gttcccttgc ggggatagag gccgagcagt tcggcagggc
     1381 gcttggtaac ggcgcagacg agccgttcga tgtcgagccc gcgtttgatg cagccttcgc
     1441 tgagcagagc gggcagatgc gtttccagcg ccggataacc ggggaaggcc ttccaaacgt
     1501 tgttcgggtc gcccttttcg cgcagg
//
LOCUS       DAOMGY010000485         1525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_98524_length_1525_cov_3.141497, whole genome shotgun sequence.
ACCESSION   DAOMGY010000485 DAOMGY010000000
VERSION     DAOMGY010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98524_length_1525_cov_3.141497"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1158
                     /locus_tag="PLR57_07490"
     CDS             142..1158
                     /locus_tag="PLR57_07490"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HQQ41337.1"
                     /translation="MQIDQIKLLRLKNQFLLNPGGAQDVVSGLIGLQAQYGAQALHAL
                     AIRSCGGFDPDTCVKTWSFRGTLHLHRKSDLPLVLYRGSQTAFGQMAFLSERVEKTRA
                     EQFRTLILDAMAERDLSRDQLKALCSAEGLTAAEEEILFHPWGGVFRALAENGEIAYT
                     SDGNRVFTRLKPLTPMEKDEALRELMRRYVKHYGPVTLRDAQTFFGMPQRELKALLEQ
                     SAGESVSIGKDTYFYIPDELPEGADMPQALFLAGFDQLLLGYRKMDNPFLPPEHIKRV
                     YNNTGIVFPTVLLHGRVSATWKRNGKVLEIRPFGKITAKDKKQIERKSVDNFGGAGVQ
                     WINN"
     gene            1228..>1525
                     /locus_tag="PLR57_07495"
     CDS             1228..>1525
                     /locus_tag="PLR57_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HQQ41338.1"
                     /translation="MITRDSRVGDLLKNPIAQDVFGRLSQYAGVSEKLLLNPIIRSLK
                     LSAIPKFAGKSIQGADQVIDTMIDLFNQEEGTKMPSPSDSRVWWKEAVVYQIYPR"
BASE COUNT          370 a          405 c          443 g          307 t
ORIGIN      
        1 aacatttccg ggcagtcctt tttttgcgcc gcgcggtata gtaagatgct ttgtaaggga
       61 catgaatcaa acgatgccgg acgaaagacg cttgaacgtc tgtttttcgc agcttaaatc
      121 gtcagattgg gtgctgaaca tatgcagatc gaccaaataa agctcctgcg gctcaaaaac
      181 cagtttctgc tgaaccccgg cggcgcgcaa gacgtcgttt cgggtctgat cggcctgcag
      241 gcgcagtacg gcgcgcaggc gctgcacgcg ctcgccatcc gctcttgcgg tgggtttgac
      301 cccgacacct gcgttaagac ctggtcgttt cgcggaacac tgcacctgca ccgcaagagc
      361 gacctgccgc tcgtgctgta ccggggcagc caaacagcat tcgggcagat ggcgtttttg
      421 agcgaacgcg tggagaaaac tcgtgccgag cagttccgca cgctgatact cgacgcaatg
      481 gcggagcggg atttatctcg cgatcagcta aaagcgctct gttcggcaga aggcctgacc
      541 gccgccgagg aggaaattct gttccatccc tggggcggcg tgttccgcgc gctggcggag
      601 aacggcgaaa ttgcgtatac gtccgacggc aatcgcgtgt tcacgcggct aaagccgctt
      661 acgccaatgg aaaaggacga agccctgcgc gaactcatgc ggcggtacgt aaagcactac
      721 gggccggtga cgctgcggga cgcgcagaca ttcttcggca tgccgcagcg ggagctcaaa
      781 gcgcttctgg aacagtcggc gggggagagc gtttctatcg gaaaagacac atatttttat
      841 ataccggatg aactgcccga aggcgcggat atgccgcaag cgctttttct cgcggggttc
      901 gaccagcttc ttctgggcta ccgcaaaatg gataacccgt ttctgccgcc ggagcacatt
      961 aagcgcgttt acaacaatac cggcatcgtg tttccaacgg tgctgctgca cgggcgcgtt
     1021 tcggcaactt ggaagcgaaa cggaaaagtt ctggagatca ggccgttcgg caaaatcacc
     1081 gcgaaagata aaaagcaaat cgagcgcaaa tccgtggaca acttcggcgg cgcgggagta
     1141 caatggatca ataactgaat aaaattaaca taagaaaaac gaaaccgagt tctattttat
     1201 aaaggatttt ggaggacgaa ttattccatg atcacgcgtg acagccgagt gggagacctg
     1261 ttgaaaaacc cgattgcgca ggacgttttt ggccgactga gccagtacgc cggggtcagc
     1321 gaaaagctgc tgctcaaccc cattatccgt tcgctcaagc tctccgccat accgaaattc
     1381 gcgggcaagt cgattcaggg cgcggatcag gtgatcgaca ccatgatcga cctgttcaat
     1441 caggaagaag gaacgaaaat gccttctccc agcgacagca gggtttggtg gaaggaagcg
     1501 gtggtctacc agatataccc gcgct
//
LOCUS       DAOMGY010000486         1525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_98527_length_1525_cov_3.114966, whole genome shotgun sequence.
ACCESSION   DAOMGY010000486 DAOMGY010000000
VERSION     DAOMGY010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98527_length_1525_cov_3.114966"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLR57_07500"
     CDS             <1..382
                     /locus_tag="PLR57_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012170138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQQ41339.1"
                     /translation="GATPKDGPSAGITMATALVSVLTGIPARSDVAMTGELTLRGMVL
                     PIGGLREKLLAAVRAGVERVVLPRANREDLYDVPAEVKSALKIEFVETVDEVLGFALT
                     MHPHGAEPHLNLPGLEITHETVRH"
     gene            363..950
                     /gene="yihA"
                     /locus_tag="PLR57_07505"
     CDS             363..950
                     /gene="yihA"
                     /locus_tag="PLR57_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQQ41340.1"
                     /translation="MKPFDIKRAEFVTSVGQGQSYPAPLACEIAIVGRSNVGKSSLIN
                     CLCNNHKLAKTSATPGKTRMINYFLLNGEWYLVDLPGYGFAKTSKTERTNWGELMENY
                     LRSGRPKHLFLLLDIRHAPSEEDKQMFRWVLYFGVPFTLIATKSDKLTKSARMLSANK
                     AAKLLGAPPFALPFSAETGEGRAELLERINQILQG"
     gene            1147..1461
                     /locus_tag="PLR57_07510"
     CDS             1147..1461
                     /locus_tag="PLR57_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41341.1"
                     /translation="MALHKCPKCELNYIRDGEDFCDVCKREMKRAQARGRHVDDETEE
                     DEVIMCTECGEAPAVRGSDLCAACLKEQKRQVELENAAELDQDYDDPIEEDEEDITPD
                     EE"
BASE COUNT          368 a          427 c          450 g          280 t
ORIGIN      
        1 aggggccacc cccaaggacg gccccagcgc cggcatcacc atggccacgg ccctcgtgag
       61 cgtcctcacg ggcatccccg cccgcagcga cgtggccatg acgggcgagc tcaccctgcg
      121 cggcatggtg ctgcccatcg ggggcctgcg cgaaaagctg cttgcggccg ttcgcgccgg
      181 cgtggaacgc gtggttctgc cgcgcgccaa ccgcgaggac ctctacgacg tacccgcgga
      241 agtgaagtcc gcgctgaaga ttgaattcgt tgaaactgtg gacgaagtgc ttggctttgc
      301 gctgaccatg catccgcatg gcgcggagcc gcacctgaat ctgccgggat tggaaatcac
      361 gcatgaaacc gttcgacatt aagcgggcgg aatttgtcac cagcgtgggg caggggcaat
      421 cttaccctgc cccgcttgcg tgcgaaattg cgatcgtcgg ccgcagtaat gttggcaaga
      481 gctcgctcat caactgcctc tgcaacaacc ataagctcgc caagacttcc gctactcccg
      541 gtaaaacccg catgatcaac tacttccttc tcaacggcga gtggtatctt gtggatttgc
      601 ccggatacgg ctttgcaaag accagcaaaa ccgagcggac caactggggc gagctcatgg
      661 agaactacct gcgcagcggc aggccaaagc atctgtttct tttgctggat attcgccacg
      721 cgccttccga agaggataag cagatgtttc gctgggtgct gtacttcggc gtaccgttta
      781 ccctgattgc aacgaagtcc gacaagctta caaaatccgc gcgcatgctc tcggccaaca
      841 aagcggcaaa gctgttgggc gcgccgccgt ttgcgctgcc gttttccgcg gaaaccggcg
      901 aaggccgcgc ggagctgctg gagcgcataa atcagattct gcagggctga cggaagggct
      961 tgtgccaaga acaatcaaac gcgcgttatt acggcggcaa cggcggaaaa aatcgccgtt
     1021 ttttgcccga aaaacgcgca aaatcgacgt catgcgcgca tggaatatgt ttatgcttga
     1081 catgcgtgtg aatcttttgc ataataacca gcgtaatgtt tggaatcaaa ggcaggttaa
     1141 atcgatatgg cattgcataa gtgcccgaaa tgtgaactaa actatatccg cgacggcgaa
     1201 gatttttgcg acgtctgcaa gcgcgaaatg aagcgcgcac aggcacgcgg caggcacgtt
     1261 gatgacgaga cggaagagga cgaagtcatt atgtgcaccg aatgcggaga agcgcccgcc
     1321 gtgcgcggaa gcgacctttg cgccgcatgc ttaaaagagc agaaacgcca ggttgaactg
     1381 gaaaacgcag cggaactgga tcaggactac gacgacccca tagaggaaga cgaagaagac
     1441 atcacgccgg acgaggaata agggaatccc cctctttcac gcggcgaaac gagaggagcg
     1501 aagggatgcg aatctacgca attgc
//
LOCUS       DAOMGY010000487         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_98803_length_1522_cov_4.102249, whole genome shotgun sequence.
ACCESSION   DAOMGY010000487 DAOMGY010000000
VERSION     DAOMGY010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98803_length_1522_cov_4.102249"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLR57_07515"
     CDS             complement(<1..361)
                     /locus_tag="PLR57_07515"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41342.1"
                     /translation="MAVSQKNGGSIKKQKTKPKAQSKFYAVFTIALAFAMSCMVTLVS
                     LTPKRYEVSVGSPSKETITAPRMVEDTEMTEALRAAARSGVKTVYSVDNKLADTLISG
                     AQSFFSAVKSFKNAAEDI"
     gene            complement(420..1388)
                     /locus_tag="PLR57_07520"
     CDS             complement(420..1388)
                     /locus_tag="PLR57_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQQ41343.1"
                     /translation="MERVSVSIPLENVDELLGLFGVFDENIGVLEEETGASIRAEASG
                     VRISGEREAVGIAQIVVEKMLQMQRRGEKFNRSRIRYAIDLAKEGNADMIEKIMGDVI
                     AITNKGRQIKCKTLGQKRYVQALKEFELVFGIGPAGTGKTYLAVAMAVQAFKNKEVER
                     IILTRPAVEAGEKLGFLPGDLQSKVDPYLRPLYDALQEFLGLESYKALYERGAIEVAP
                     LAYMRGRTLNNAYIILDEAQNCSSEQMKMFLTRIGEGSRVVVTGDVTQIDLPKEKISG
                     LVQAVKVLDNVEGIRIINLTHKDVVRHELVQRIICAYERFEKPKRS"
BASE COUNT          303 a          445 c          375 g          399 t
ORIGIN      
        1 tgatgtcctc cgccgcattc ttaaaactct tgaccgcgga aaagaagctc tgcgcgccgg
       61 aaatcagcgt gtccgcaagc ttgttgtcta ctgaataaac ggtcttgacc ccgctgcgcg
      121 cagccgcacg gagcgcctcg gtcatttcgg tatcttccac catacggggc gccgtaatcg
      181 tttccttgga agggcttcct acggaaactt cataccgctt gggcgtaagc gaaacaagcg
      241 ttaccatgca gctcatcgca aacgcgagcg caatggtaaa cacagcgtaa aatttggact
      301 gcgctttggg ttttgtcttc tgctttttta tactgccgcc gttcttttga gaaactgcca
      361 cgattctttc tgtccttttc tacgtaatct ttcgcctgaa agggcaatcg ggcgatgatt
      421 caggagcgct tcggtttttc aaaccgctcg tacgcgcaga tgatgcgctg caccagttcg
      481 tggcgcacca cgtccttatg cgtcaggttg ataatccgga ttccctccac attgtcgagc
      541 acttttacgg cctgcacgag accgctgatc ttctctttcg gcaggtcgat ctgcgtaacg
      601 tctccggtga cgaccacgcg ggaaccttcg ccgattcggg ttaaaaacat cttcatctgc
      661 tcgctggagc agttctgcgc ttcgtcgaga atgatatagg cgttgttgag cgtgcgcccg
      721 cgcatatagg caagcggagc cacttcaatt gcgccgcgct catacagcgc tttgtagctc
      781 tccagcccta aaaactcctg cagcgcgtcg tagagcggac gcaggtaagg atcgaccttg
      841 ctctgcaggt cgcccggcaa gaagccgagc ttctctcccg cttctacggc tggacgggtt
      901 aaaataatcc gttcaacttc cttattctta aacgcctgca ccgccatggc aaccgcgaga
      961 tacgttttgc ccgtgccggc ggggccaatg ccgaatacca gttcaaactc cttgagcgcc
     1021 tgaacatacc gtttctggcc gagcgttttg catttaatct gacgtccttt gttggtaatg
     1081 gcaatgacgt cccccatgat cttttcgatc atgtccgcat tgccttcctt ggccaggtcg
     1141 atggcgtaac ggatgcggct gcggttgaac ttttcgccgc gacgctgcat ttgaagcatc
     1201 ttctccacaa cgatctgggc gatgcctacg gcttcccgtt cgccgctgat gcgtacgccg
     1261 cttgcttccg cgcggatgct tgcgccggtt tcctcttcca gcacgccgat attttcatca
     1321 aacacgccga acagtcccag cagttcgtcc acattttcca gcgggatgga aacgctgacc
     1381 cgctccattt cctgtgtcaa acagtattct ccttcggtta tcgtaaagtc aattgaacat
     1441 atgaaccatg cttgtgatcg cgccgttttt tgcgcattta ccgctgttta gggcgattat
     1501 aacataccct cgcgggaaat ac
//
LOCUS       DAOMGY010000488         1521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_98944_length_1521_cov_2.828786, whole genome shotgun sequence.
ACCESSION   DAOMGY010000488 DAOMGY010000000
VERSION     DAOMGY010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98944_length_1521_cov_2.828786"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLR57_07525"
     CDS             <1..385
                     /locus_tag="PLR57_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41344.1"
                     /translation="GTGKTKQILEMANKSVQTAKGNIIFIDDDNGYMYDLSTKARFIN
                     ASEYSITTPKMLYGFLCGLAAGDFDLETIYIDGLLSIIHHSLDTLEGLFTELKQFSDK
                     YSVNVIMSITGNKQTTPDFLKDFVL"
     gene            428..>1521
                     /gene="thrC"
                     /locus_tag="PLR57_07530"
     CDS             428..>1521
                     /gene="thrC"
                     /locus_tag="PLR57_07530"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQQ41345.1"
                     /translation="MRYLSTRGGEPVRAAEAIVRGISPSGGLYVPETYPKVNVETGER
                     GSYQATALRIMQAYFPEAEESALKGMIGAAYASFDAKEVTPLVQLTGAESVLELWHGP
                     TMAFKDVALQMLPHLMLESLRQTGEQKRVYILVATSGDTGKAALEGFKDVPGTRVLVF
                     YPHGGVSEAQRLQMVTQEGGNMDVCAVRGNFDDAQTAVKQIFASKEIAAELEKLGWRL
                     SSANSINFGRLLPQIVYYYTSYQTLLERGTIRPGERVNFCVPTGNFGDILAGEYARRM
                     GLPVNKLICASNRNNVLTDFFTRGVYDDKRPFYKSMSCSIDILISSNLERLLFELAGR
                     DDAVVRGWMRDLKEQGMYALPKEALDELGKG"
BASE COUNT          362 a          422 c          434 g          303 t
ORIGIN      
        1 aggcacgggc aaaaccaagc aaatcctcga aatggcaaac aaatctgtcc agactgccaa
       61 gggaaacatc atcttcatcg acgacgacaa cggctatatg tacgacctct ctacgaaagc
      121 tcgcttcatt aacgcatcgg aatacagcat cacgacaccg aagatgcttt acggattcct
      181 ctgcggtctt gcagcgggcg attttgatct tgagacgatc tatatcgacg gactgttaag
      241 tatcattcat cactctctcg atacgctgga aggactgttt accgaactga agcagttttc
      301 cgacaagtac tccgtcaacg taatcatgag cattaccggc aacaagcaga cgactcccga
      361 ctttttaaag gacttcgtac tgtaaccgac cggaacatta taaaagaata aaggatatca
      421 ttgacccatg cgttacctga gtaccagggg cggtgaaccg gtgcgcgcgg cggaagccat
      481 cgtgcgcggc atcagcccgt cgggcggact gtatgtgccc gaaacatatc ccaaagtgaa
      541 tgtggaaacc ggcgagcgcg gcagctacca agccaccgcg cttcgcatca tgcaggcgta
      601 ttttccggaa gcggaagaaa gcgctttaaa aggcatgatc ggcgccgctt acgcgagttt
      661 tgacgcaaaa gaagttacgc cgctcgtaca gctgaccggt gcggaatccg tgctggaact
      721 ctggcacggg ccgacgatgg cgtttaagga cgtagccctg cagatgctgc cgcacctgat
      781 gctggagtcc ctccggcaga caggggagca gaagcgagtg tatatcctcg ttgccacttc
      841 cggcgatacc ggcaaagccg cgctggaagg gtttaaagac gttcccggca cgcgcgttct
      901 ggtgttctac ccgcacgggg gcgtatcgga agcacagcgc ctccaaatgg ttacccagga
      961 gggcggcaat atggacgtct gcgccgtgcg cggcaacttt gacgacgcgc agaccgcggt
     1021 caagcagatt ttcgcgtcga aggagatcgc tgccgaactg gaaaagctcg gctggcggct
     1081 ttcgagcgcg aactccatca actttggaag gctcctgccg cagattgtgt attactacac
     1141 gagctatcag acgcttctgg agcgcggaac gattcgtccg ggcgagcgcg tcaatttctg
     1201 cgtgccgacc ggcaactttg gcgatattct cgcgggcgaa tacgcgcgca ggatgggact
     1261 gccggtcaac aagctcattt gcgcctccaa ccgcaacaac gtgctcacgg acttctttac
     1321 gcgcggggtc tacgacgaca agcgcccgtt ctataagagc atgagctgct cgatcgatat
     1381 ccttatctcc tccaatctgg agcggctgct ctttgagctc gcagggcgcg acgacgcggt
     1441 tgtgcgcggc tggatgcgcg acctgaagga acagggcatg tacgctctgc cgaaggaagc
     1501 gctggacgaa cttggcaaag g
//
LOCUS       DAOMGY010000489         1520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_99031_length_1520_cov_3.592491, whole genome shotgun sequence.
ACCESSION   DAOMGY010000489 DAOMGY010000000
VERSION     DAOMGY010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99031_length_1520_cov_3.592491"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLR57_07535"
     CDS             complement(<1..873)
                     /locus_tag="PLR57_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HQQ41346.1"
                     /translation="MNKLKDAVRFFGLSMLTMVVIAMAFLGFRSQMKAQLLENTFQRL
                     TEAAQQQADTIRTFFDVTKKRMEIIAKYGSSPAHRAVVEGMLGELTGGQGMAWAALVD
                     QDGTLKLENGTTRNVSSEDWFQTCLDGESVIAVGRTGELMPSGILVAVPVKENGFTVG
                     ALVARMDRETISGLTQTSAFEGFTYALLSDAAGNVFAVADQTTQETYSINLFDEFVSD
                     AALQGDMTEAKLKESFQNGETTSTTTLRGGKTYYSVNVPIGVADWFFVLAVPSTVADE
                     LSAKIITYVLVMLVL"
     gene            complement(1196..>1520)
                     /locus_tag="PLR57_07540"
     CDS             complement(1196..>1520)
                     /locus_tag="PLR57_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HQQ41347.1"
                     /translation="YIEVANMPTTNEALGYLMAQKHLIIAPSKAVNAGGVATSALEMS
                     QNSMRYSWTAEEVDAKLHAIMKNIHDNSAKAAKENGLGYNLVAGANIAGFAKVADAMI
                     AQGIF"
BASE COUNT          331 a          430 c          372 g          387 t
ORIGIN      
        1 cagaacgagc atcacgagta cataggtgat aatctttgcg ctcagctcgt cggctacggt
       61 gctcggaacc gccagaacga agaaccagtc cgcaactcca atcggaacat tgaccgagta
      121 gtaggttttt ccgccccgta atgtcgtcgt tgaagttgtt tcgccgtttt gaaacgactc
      181 tttgagcttt gcctcggtca tatccccttg cagcgccgcg tcggaaacaa actcatcaaa
      241 aagattaatc gagtaggttt cctgcgtcgt ctgatccgca accgcaaaca cgttgccggc
      301 tgcgtccgaa agcagcgcat aggtaaatcc ttcgaacgcg ctggtttggg tcaggccgga
      361 tatcgtttcg cgatccatgc gcgcaacgag cgctccgaca gtaaaaccgt tttcctttac
      421 gggaaccgca accagaattc cgctcggcat cagttcgccg gttcgcccga ccgctatcac
      481 actttctccg tccaggcagg tttggaacca atcctcgctg gagacgtttc gcgttgtgcc
      541 gttttcaagc ttcaaggttc cgtcctgatc gaccagcgcc gcccatgcca tgccttgtcc
      601 gcctgtcagt tcgcccagca ttccctccac aacggcgcga tgcgccggac tggaaccata
      661 ttttgcgatg atctccattc gttttttcgt aacgtcgaaa aatgtgcgaa tcgtatcggc
      721 ctgttgctgg gctgcttccg tgagccgctg aaaggtgttt tccagcagtt gcgctttcat
      781 ctgcgaacga aaacccagaa acgccatcgc gatgacaacc attgttagca tagacaagcc
      841 aaaaaaacgt acggcgtctt tcagcttatt cataccgact ccgccccttt catgagagag
      901 atgacgcttt aacggttgct gcaatttgta gagtgtatgg gttcgttttc gctgaagtga
      961 attacaaggt ataataaaat tatatacaca attgattgct ttgtcactta tatttaccgg
     1021 tgctgaataa tcccaacggg taccggtaaa aaagcgtccg tgctgaacaa aaagcgcccg
     1081 cttctgaaag gaaatgggcg tacattgccc cttaaaagat tccctgcgca atcattgcgt
     1141 ctgctgtgaa taaaaaagcg cccgcttccc tccggaagcg ggcgttccat attcgttaga
     1201 aaattccctg tgcaatcatg gcgtctgcga ccttggcaaa ccccgcgatg ttcgcgcccg
     1261 caaccaggtt gtagccaagc ccgttttcct tggccgcttt ggcagaattg tcgtggatgt
     1321 ttttcatgat tgcatgaagc ttagcgtcga cttcttccgc cgtccagctg tagcgcatcg
     1381 agttctggct catctccagc gcgctcgttg ccacgccgcc cgcgttgacc gccttgctgg
     1441 gcgcgatgat caggtgcttc tgagccatca ggtaccccag cgcttcgttc gtcgtgggca
     1501 tgttcgccac ttcgatgtag
//
LOCUS       DAOMGY010000490         1510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_99978_length_1510_cov_3.585567, whole genome shotgun sequence.
ACCESSION   DAOMGY010000490 DAOMGY010000000
VERSION     DAOMGY010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99978_length_1510_cov_3.585567"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLR57_07545"
     CDS             <1..363
                     /locus_tag="PLR57_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41348.1"
                     /translation="GEPTARGKLTKLCESALFYLARDKEFCDLLKNDPSIFPMFPTVD
                     PYEEINGESVRMIARILAFGRETGEFQSVDAEGAAEVLFSIYKNFIIHTYINAEEDYT
                     NRYLPITLDLLLGGILKK"
     gene            501..893
                     /locus_tag="PLR57_07550"
     CDS             501..893
                     /locus_tag="PLR57_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HQQ41349.1"
                     /translation="MVKYLSEEWRAEVEKRLSEQLTPEKMNNLTSSMNNLYLNCPDGQ
                     SHYFFVGFVDGKVDSVQVGAGEGPKAEFVISGEYETFAKISRAELGAQKALMGGLLKL
                     KGNMVKALKLASLVDRLNKIIATIETEY"
     gene            907..>1510
                     /locus_tag="PLR57_07555"
     CDS             907..>1510
                     /locus_tag="PLR57_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HQQ41350.1"
                     /translation="MFDKTSPYYIDFPGRIKAIQAEMAKEKIDLYLGSRLRTMSWTTD
                     AFFPWRSFIVIPADGLPTAFTFVIDAARAADDSWLDEDHVMGFAPMGGQDQITQISDL
                     IKEYLPHGGRVGVEAGMSNYLPEGYLTYYEYKAFEAALPGCTLVNAHPIVDRLQMIKD
                     IGTINRFREASRIVDIGHEAVLAAIKNGGWKGMTETEIGGL"
BASE COUNT          351 a          450 c          417 g          292 t
ORIGIN      
        1 ggcgaaccca ccgcgcgcgg caagctgacg aagctctgcg aaagcgcgct gttttacctg
       61 gcgcgggata aagagttctg cgacctgctc aaaaacgacc cctcgatctt tccgatgttc
      121 ccgaccgtcg atccctacga ggagatcaac ggagaatccg tgcggatgat cgcgcgtatc
      181 ctcgcttttg gccgcgagac cggtgaattc cagagcgtgg acgcggaagg cgcggcggag
      241 gtgctgtttt ccatctacaa gaatttcatc atccatacct atatcaacgc ggaggaagat
      301 tacaccaacc gctacctgcc catcacgctt gaccttctgc tcggcggcat cctcaaaaaa
      361 taaccttccc gcgcaaaccg ctttgccctc ttaagaaaga cgcatcatac ccagccggct
      421 tttccgccgc tgaagcggcg ttttcaccat ttctagcaaa aaagcacatc caagtaaata
      481 tcgaaaggag catttcaacc atggtcaagt atctgtccga agaatggcgc gcggaggtcg
      541 aaaaacggct cagcgagcag ctcacccccg aaaagatgaa caaccttacc tcctctatga
      601 acaacctcta cctcaactgc ccggacggcc agtcgcacta tttcttcgtg ggctttgtcg
      661 atggcaaagt cgacagcgtt caggttggtg cgggcgaagg cccgaaggcg gagtttgtga
      721 tctccggcga gtatgaaacg ttcgccaaga tctcccgcgc ggagctcggc gcgcagaagg
      781 cgctgatggg cggcctcctg aaactcaagg gcaatatggt caaggcgctg aagctcgctt
      841 ctctggttga ccgcctcaac aagatcatcg cgaccatcga gacagagtat tgaggaggaa
      901 atagcaatgt ttgataaaac aagcccttat tatatcgatt ttcccggccg catcaaggcc
      961 attcaggccg agatggccaa ggagaagatc gacctgtact taggctcccg cctccgcacc
     1021 atgagctgga cgacggacgc gttcttcccc tggcggagct ttatcgtcat tcccgccgac
     1081 gggctgccca cggcgtttac gttcgtcatc gacgcggcgc gcgcggcgga cgacagctgg
     1141 ctggacgaag accacgtgat gggctttgcg cccatgggcg ggcaggatca gattacgcag
     1201 atttccgacc tcatcaagga atatctgccg cacggcggac gggtcggcgt ggaagccggt
     1261 atgtcgaact acctgccgga agggtacctg acctattacg aatacaaagc gtttgaagcg
     1321 gctctgccgg gctgcacgct cgtcaacgcg catccgatcg ttgaccgcct gcagatgatc
     1381 aaggacatcg gcaccatcaa ccgcttccgc gaagcttccc gcattgtgga catcggccac
     1441 gaggcggtgc tcgccgccat caaaaacggc ggctggaagg gcatgaccga aaccgaaatc
     1501 ggcggcctcg
//
LOCUS       DAOMGY010000491         1509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_100083_length_1509_cov_3.594223, whole genome shotgun
            sequence.
ACCESSION   DAOMGY010000491 DAOMGY010000000
VERSION     DAOMGY010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100083_length_1509_cov_3.594223"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..>1509)
                     /locus_tag="PLR57_07560"
     CDS             complement(90..>1509)
                     /locus_tag="PLR57_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQQ41351.1"
                     /translation="TFRGNNYRDSANYGIASVLNRKMTEAWQVQTSELKCGLSTSDPE
                     KTWTGSGWVGQPLIVKWPDNIKQVMNMYDWAKQKEGLVEVILATMDGNVYFVDLETGE
                     PTRDKLVIGMPFKGAGSLDPRGYPILYLGSGDMYANDSQMARAMAYSLIDFSRLYEFG
                     KQYDEFALREWHAYDSAPLVDAETDTLIYPGENGILYTIKLNTKFDETTGELSMNPSY
                     IVKMRYVASRSTYPASSDNKYWLGYETSIATLGEYGYLGSNDGFLQCINLNTMSVVWV
                     QDTKDDTNGSPVLETDFANRTAYLYIGSSLHFTADADRKGTVSLYKINAITGEIVWEH
                     PENVNTKSGVSGGIQATALLGKGNISDLVIIPFARTPNEDDGVVLALDKATGAVRWTF
                     VMEKYTWSSPVAVYDENGNAYIVVCEGSDTGGKIFLLDGKTGTKLATFEAEKNIEASP
                     AVYGNMIVIGTRGMKIWGVKIS"
BASE COUNT          330 a          431 c          372 g          376 t
ORIGIN      
        1 cgtgaaattc aaatccgccg ataaaatgcg cttcaccccg gcggaaaggc atccgctctc
       61 cgccggggca acgattgctt caagaaatac tagctgattt taacgcccca tatcttcatg
      121 ccgcgcgtac caataacaat catgttccca tagacggcgg gagacgcttc aatattcttc
      181 tctgcttcga aggtggccag tttcgtgccg gttttgccgt cgagcagaaa gatttttccg
      241 cccgtatcgc tgccttcgca gacgacgatg taagcgttgc cgttttcatc atacaccgca
      301 accggagacg accaggtgta tttttccata acgaacgtcc agcgcaccgc gccggtcgcc
      361 ttgtccagtg ccagcaccac gccgtcgtcc tcgtttggcg tacgcgcaaa cgggatgatc
      421 acaaggtcgg agatattgcc cttgcccagc agcgccgttg cctgaatacc gccggagaca
      481 cctgatttcg tattaacgtt ttccgggtgc tcccaaacaa tctctccagt tatcgcgtta
      541 atcttgtaaa gcgagacggt tccttttcta tccgcatctg ctgtaaagtg cagagacgaa
      601 ccaatataga gatacgctgt ccggtttgca aagtcagttt ccagaacggg cgaaccgttc
      661 gtatcgtcct tggtgtcctg cacccagacg acggacatcg tgttgaggtt gatgcactgc
      721 aagaacccgt cgtttgaacc gagatatccg tattcgccca gtgtggcgat gctggtttcg
      781 tagccgagcc aatatttatt gtcggaactg gccggatacg tcgagcggga agcgacatag
      841 cgcatcttaa cgatgtagct cgggttcatc gagagttcgc ccgtggtttc gtcgaacttc
      901 gtgttgagct tgatcgtgta cagaatcccg ttctcgccgg ggtaaatcag cgtatcggtt
      961 tccgcatcca ccagcggcgc gctgtcgtaa gcatgccatt cccgcaaggc aaattcatca
     1021 tactgcttac caaactcata aagcctggag aaatcgatca gagaatacgc cattgcacgc
     1081 gccatctgcg aatcatttgc gtacatatcc cccgaaccaa ggtacagaat gggatatccg
     1141 cgcggatcaa gcgaaccagc acctttgaac ggcatgccga tcacgagttt gtcgcgcgtt
     1201 ggttcacccg tctccaaatc gacaaaataa acattgccgt ccattgtcgc gagtatgact
     1261 tcgacaaggc cttccttctg ctttgcccag tcgtacatgt tcataacctg tttgatatta
     1321 tccggccact tgacaatcaa gggttgacca acccagccgc tccccgtcca tgtcttttcc
     1381 gggtccgatg tgctgagtcc gcatttcagt tcgctcgtct gtacctgcca tgcttcggtc
     1441 atcttgcggt tgagcacgct ggcaatgccg tagttcgcgc tgtcgcgata gttgttgccg
     1501 cggaacgtg
//
LOCUS       DAOMGY010000492         1507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_100308_length_1507_cov_2.891185, whole genome shotgun
            sequence.
ACCESSION   DAOMGY010000492 DAOMGY010000000
VERSION     DAOMGY010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100308_length_1507_cov_2.891185"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PLR57_07565"
     CDS             <1..1054
                     /locus_tag="PLR57_07565"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQQ41352.1"
                     /translation="EVTAAFLDLLQNSDPMFLHGYTSAPGSNEAREAIAASLNRRFGT
                     DFSRRNFYLTCGAAAALVSIFRALTIDQNSEFVAFAPFFPEYACFATAAGGRLRVVEA
                     DRKGFQIDFCQLERVLSFYTQGVIINSPNNPSGVIYSEATLRQLADLLRKKSMEYGHP
                     IYLISDEPYRELTYKEVTVPFITQLYDCSIVSYSYSKSLSLPGDRIGYVLVGDAMPDF
                     DDVCNAISGSARKQGYVCAPSILQRVVARCSQLDLMPDLSSYKRNRDALYNGLTQIGY
                     RCVPPDGAFYLFVQAPNGDGDAFAELAKSEEILIVSGREFGCPDYVRISYCVSYETIV
                     NALPGFRRIFAQVCNN"
     gene            1127..>1507
                     /locus_tag="PLR57_07570"
     CDS             1127..>1507
                     /locus_tag="PLR57_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41353.1"
                     /translation="MCQEDKAPHITDPSAAAKLAAREQAKIQLELETRYELIEKILHL
                     LERKAALKSEIEHLKTALENERGWNRISLSFLISGVLFGVVLSPGFQYLWIIPVALFG
                     SLIVGFFIGFLLSRIQAVIRSQIYE"
BASE COUNT          307 a          441 c          400 g          359 t
ORIGIN      
        1 ggaagtcacc gcggcgtttc ttgacctgct gcaaaacagc gatcccatgt ttctgcacgg
       61 ctataccagc gcgccgggca gcaacgaagc gcgcgaagca attgccgcct cgctcaaccg
      121 tcgattcggc accgattttt cgcgcagaaa cttctacctc acctgcggcg cggcggcggc
      181 gctcgtttcc attttccgcg cgctgaccat cgaccaaaac tcggaatttg tcgcgtttgc
      241 gccgttcttt ccggagtacg cctgctttgc aacggcggcg ggcggacgac tgcgcgtggt
      301 ggaagcggac cggaaaggct tccaaatcga tttctgtcag ctcgaacgcg tgctttcgtt
      361 ctatacgcaa ggcgttatca tcaactcgcc caacaatcct tccggcgtga tctattcgga
      421 agcgaccttg cgacagctgg cggatcttct gcgtaaaaaa agcatggaat acggacatcc
      481 gatctacctg atttcggacg agccgtaccg cgagctgacg tataaagaag taaccgtacc
      541 gttcatcacg cagctctacg actgctccat cgtatcctac tcctactcca agagcttgtc
      601 cctgccgggc gaccgcatcg gctatgtgct cgttggcgac gccatgccgg actttgacga
      661 cgtttgcaac gccatctccg gcagcgcgcg taagcagggc tacgtctgcg cgccgtccat
      721 cctccagcgg gtggttgcgc gctgttcgca gctcgacctg atgcccgatt tgagcagtta
      781 caagcgaaac cgcgacgcgc tctacaacgg tttgacgcaa atcggttacc gctgcgttcc
      841 gccggacggc gcgttttacc tctttgttca ggcgcccaat ggcgacgggg acgcctttgc
      901 cgagctggca aagagtgaag aaattctaat cgtctccggc agagagttcg gctgtccgga
      961 ctacgtgcgc atttcctatt gtgtttccta cgaaaccatc gtcaacgcgc tgcccggttt
     1021 tcggcggata tttgcacaag tgtgcaataa ctgatataat ctttttatca ttgatgccac
     1081 ctgtactttc tcgttttacc ccggctggac tcggaggaaa aaacgtatgt gtcaggagga
     1141 taaagcgccc catatcacag acccttccgc ggcggcaaag ctcgcggcgc gcgaacaggc
     1201 caagattcaa ctggagctgg aaacgcggta tgagctcatc gagaagatat tgcatctgct
     1261 cgagcgtaaa gccgctctga aatcggaaat cgagcatcta aaaactgcac tggaaaacga
     1321 gcgcggctgg aaccgaatct cgctttcctt tttaatttca ggcgtgttgt tcggcgttgt
     1381 gctttccccc ggttttcagt atctttggat tatccctgtc gcgctgttcg gctcgctgat
     1441 tgtcgggttt ttcattggct ttctgctcag ccggatacag gccgtcatcc gctcacagat
     1501 ctacgag
//
LOCUS       DAOMGY010000493         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_100470_length_1505_cov_5.583448, whole genome shotgun
            sequence.
ACCESSION   DAOMGY010000493 DAOMGY010000000
VERSION     DAOMGY010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100470_length_1505_cov_5.583448"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PLR57_07575"
     CDS             complement(<1..984)
                     /locus_tag="PLR57_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HQQ41354.1"
                     /translation="MLRKSIVLLMVLLLAFSFACKPKAEEPAVAEVTPTLAPTMEPAA
                     LDKDLAILYTNDVHCAVDSGIGYAGVAALKQQLLDDGCYVALVDVGDAVQGDAIGTLS
                     KGSYIIDIMNEVGYDVATIGNHEFDYGMNRFFELREMAKFPYVSANFVDLASGAPVLE
                     PYTMLTFNGVKVAFVGVSTPFTITSSTPAYFQDANGNFIYGFSQANGPEAFYAVVQAA
                     VDSAAAEGADYVIALTHLGIDASNSPYTSSELIENTTGIDIVIDGHSHSVIECERVKN
                     KDGTRVLLTQTGTKLESVGMAYITKDGSLSTGLISDFAEKDADTEAFIAQIQ"
     gene            1172..>1505
                     /locus_tag="PLR57_07580"
     CDS             1172..>1505
                     /locus_tag="PLR57_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQQ41355.1"
                     /translation="MDRPVYQNVVHLTDYGAPYEGNFVSSLRALEAKLNAEGRAMAYV
                     FPPRAAKQLWAYEMVRNKPNVTILKPGGFFSYARQVRKLLKETKSGVLHVHFIHYRDK
                     IASLLAART"
BASE COUNT          335 a          421 c          390 g          359 t
ORIGIN      
        1 ctgaatctgc gcgataaacg cttcggtatc cgcatccttt tcggcaaaat cgctgatcag
       61 gccggttgag aggctgccat ctttggtgat gtatgccatg ccgacggatt cgagcttcgt
      121 gccggtctgg gtcagcagca cgcgcgtgcc gtccttgttt ttcacgcgct cacactcaat
      181 aacgctgtgt gaatgcccgt cgatgacgat atcgatgccg gtggtgtttt caatcagttc
      241 gctcgacgta tagggcgagt tggaagcgtc tatgccaagg tgcgtcagtg cgataacgta
      301 gtccgcgcct tcggcagccg cgctgtcgac cgcagcctga acgaccgcgt aaaacgcttc
      361 cggcccgttc gcctggctga agccgtagat gaagtttccg ttcgcgtcct gaaaatacgc
      421 cggcgtggag gaagtaatcg taaacggcgt agaaacgccg acgaacgcaa ctttcacgcc
      481 gttgaacgta agcatcgtgt acggttccag cacgggagcg ccgctggcaa gatcaacgaa
      541 gtttgccgag acatacggga atttcgccat ctcccgcagc tcgaagaacc ggttcatgcc
      601 gtagtcgaac tcgtggttgc caatcgttgc aacatcgtag ccgacttcgt tcataatgtc
      661 gataatgtaa gagcccttcg atagcgtgcc gattgcgtcg ccctgtacgg cgtcgcccac
      721 atcgaccagc gctacatagc atccgtcgtc aagcagttgt tgtttgagcg cggcaacacc
      781 ggcgtaaccg atgccgctgt ccaccgcgca gtgcacgtcg ttggtataca gaatcgcaag
      841 atccttatcc agcgcggcgg gttccatagt cggggcaagc gttggagtta cttccgcaac
      901 cgcgggctct tcggccttcg gtttgcaggc aaacgaaaac gcaagcaaga gcaccatgag
      961 cagcacaata ctttttcgca gcatgttcaa tgtttccctc cttttctatg atactctgac
     1021 tatatcatat tcccagccgt gattggtttt ttacacactg tttcagaaaa tatattctcc
     1081 cgcagccttc aagccgatat tttacctgtc gcagtgcttt tctttcctgt ataataaata
     1141 tggttcattt taactgaaag gcggagaaca aatggatcgg cccgtatacc agaacgtagt
     1201 gcatcttacc gactacggcg cgccgtacga gggcaacttt gtttcgtcgc tccgcgcgct
     1261 ggaagcaaaa ctgaacgccg aaggacgcgc catggcctat gtgttccccc cgcgcgcggc
     1321 aaagcagctt tgggcttatg aaatggtgcg taataaaccg aatgtaacga tactaaaacc
     1381 cggcggtttc ttttcgtacg cccgacaggt gcgcaaactc ctgaaggaaa caaagtccgg
     1441 cgttctgcac gttcacttta tccattaccg agataagatc gcttcgcttc tcgccgcgcg
     1501 cacca
//
LOCUS       DAOMGY010000494         1504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_100580_length_1504_cov_3.594203, whole genome shotgun
            sequence.
ACCESSION   DAOMGY010000494 DAOMGY010000000
VERSION     DAOMGY010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100580_length_1504_cov_3.594203"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PLR57_07585"
     CDS             <1..1020
                     /locus_tag="PLR57_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HQQ41356.1"
                     /translation="FGNMDTWLIWNLTGVHVTDYSNASRTMLFDIHKLDYDEKLLDRL
                     NIPRKMLPKAMPSSCVYGEIKHHILDLEALGGVPVAGAAGDQQAALFGQACFEKGSAK
                     CTYGTGGFLMMNTGTEPIMSENRLLTTIAWGVGGRVEYALEGSIFNAGSSIKWLRDDL
                     GLISSAREVDTLAESVPDAGGAYFVSAFTGLGAPHWDMYARGAMLGLTRATTKAHFAR
                     AVLEGIAYQVRDLAETMEKDAKTPLAELKVDGGASVSNIMMQFQSDVLNTRVNRPKCV
                     ESTALGAACLAGLAAGVFKDTEAIARQWQSERIFTPQMSAGERETRLSGWRRALSRAM
                     SWEEK"
     gene            1284..1357
                     /locus_tag="PLR57_07590"
     tRNA            1284..1357
                     /locus_tag="PLR57_07590"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1316..1318,aa:Gly,seq:ccc)
BASE COUNT          323 a          399 c          469 g          313 t
ORIGIN      
        1 tttggcaata tggatacctg gttgatctgg aacttgaccg gcgtgcacgt taccgactat
       61 tccaacgcat cgcgcacgat gctctttgat attcataagc ttgactatga cgaaaagctt
      121 ctcgaccgtc tcaatattcc ccgcaaaatg ctgccaaagg cgatgccttc gagctgcgta
      181 tacggcgaga tcaagcatca tattctcgat ctggaagcgc tcggcggcgt gcctgttgcg
      241 ggcgcggcag gcgaccagca ggcggcgctt ttcgggcagg cctgctttga aaagggctcg
      301 gcaaagtgca cctacggcac gggcgggttc ttgatgatga acaccggaac cgagccgatc
      361 atgagcgaaa accggctgtt gactaccata gcgtggggtg ttggcggaag agtggagtac
      421 gcgctggaag gcagcatctt caacgcgggc agctcgatta agtggctcag ggacgatctt
      481 ggccttatct ccagcgcgcg cgaggtcgat acccttgcgg aatccgtgcc ggacgcgggc
      541 ggggcgtact tcgtttccgc gtttaccggg cttggcgcgc cgcactggga tatgtacgcg
      601 cgcggcgcca tgctggggct gacccgcgct accacgaagg cgcattttgc gcgcgcggtg
      661 ctggaaggaa tcgcctatca ggtgcgtgac ctcgccgaaa ccatggaaaa ggacgcaaag
      721 acgccgcttg ccgagctcaa ggtggacggc ggggcttccg tttccaacat catgatgcag
      781 ttccagtcgg acgtgcttaa tacgcgcgtc aaccgcccaa agtgcgtgga atccacggcg
      841 ctgggcgcgg cttgccttgc cggtcttgcc gcaggcgtat tcaaagacac cgaagcgatt
      901 gcgcggcaat ggcagagcga gcgcattttt acgccgcaga tgagcgccgg cgaacgcgaa
      961 acgcgccttt cgggatggcg gcgcgcgctc tcccgcgcga tgagctggga agaaaagtaa
     1021 agcttcgggt ttttgctgga atcatgcggt gatttcgaga agcgcgtccc gtatcttgcg
     1081 ggtacgggac gcgccgaata cacgatatgg atgttgactt ttcttcgcaa agtctataga
     1141 atattgggtg ctatcgtgac ggcgtgtggg taacgcatac gcttttatcg atgcggcttt
     1201 tccggcgcgc aaaaacagga acgcgtttga aaaaccggta ctattcgctg aacgcgccgg
     1261 cggcgagggg attagcacgg cgtgcgggtg tagctcagtg gcagagctcc agcttcccaa
     1321 gctggctatg tgggttcgat tcccatcacc cgctccagca tccgaaaaag gtgcacaaat
     1381 tattatgccc gctgccgtta caaattccgc atgcgggtaa caacagattt tttaggagga
     1441 ttggttgtaa tggcaaaggc aaaattcgag agaacgaaac cgcacgtcaa catcgggacg
     1501 atcg
//
LOCUS       DAOMGY010000495         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_4964
            NODE_100894_length_1501_cov_2.952974, whole genome shotgun
            sequence.
ACCESSION   DAOMGY010000495 DAOMGY010000000
VERSION     DAOMGY010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867123
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:07:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,520
            CDSs (total)                      :: 1,509
            Genes (coding)                    :: 1,494
            CDSs (with protein)               :: 1,494
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 8 of 15
            Pseudo Genes (incomplete)         :: 5 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100894_length_1501_cov_2.952974"
                     /isolate="AMR_MDS_4964"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /gene="mmsA"
                     /locus_tag="PLR57_07595"
     CDS             complement(<1..1274)
                     /gene="mmsA"
                     /locus_tag="PLR57_07595"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HQQ41357.1"
                     /translation="MNIQRLKPYINGAFVESKTAKYMPVYDPSTGETIAETPCCTVDE
                     VNAAVAAAEAAFPAWSNTPCAKRVEVLYRFRDLLIAHLDELTHILCRENGKNWDEARG
                     DLLKVKEPVEVAIGAPSMMMGESLMNVTSGFDSTLYREPLGVFVGIAPFNFPAMIPMG
                     WMMPMCIATGNTLVLKAASMTPMTSMRLMELLTEAGLPNGVVNIVTCSRVEAELLLSH
                     PDVAGVTFVGSTKIGKHIYATSAANGKRVSCLCEAKNHALVLEDAPIARTAAGILNSA
                     FGCAGERCMALPVIVALDSIADELVSELLKQVKGKKIGPAYDKTTDLGPVVTAEHRQS
                     VLDWIETGIREGAELVLDGRSVSVPGYENGFYLGHTIFDHVTPEMSVGNSEIFGPVLC
                     IKRVKDFEEGLALMNQSPFANGSVIFTQNGYYA"
     gene            complement(1318..>1501)
                     /locus_tag="PLR57_07600"
     CDS             complement(1318..>1501)
                     /locus_tag="PLR57_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HQQ41358.1"
                     /translation="LGSAVAEALGETHPVPMERIGIKESFGEVGSQDWLMDHFGLRAE
                     NIAQAAIRVIERKKNQ"
BASE COUNT          273 a          428 c          458 g          342 t
ORIGIN      
        1 gcgtagtagc cattctgtgt aaagatgact gaaccgttgg caaaggggct ttgattcatg
       61 agcgcgagac cctcttcaaa gtccttgacg cgcttgatgc agagcaccgg cccgaagatt
      121 tcgctgttgc cgacgctcat ttccggcgtc acgtggtcaa agatggtatg tccgagataa
      181 aagccgtttt catagccggg tacggatacg ctgcgcccgt cgagcacgag ctccgcgccc
      241 tcgcggatgc ccgtctcaat ccagtcgagc acactctgcc ggtgttccgc cgtaacgacg
      301 gggccgaggt cggtcgtttt gtcgtaggct ggcccgatct ttttgccctt gacctgcttg
      361 agcagttcgc tcacaagctc gtcggcaatg ctgtctaacg cgacgatgac cggcagcgcc
      421 atgcagcgtt cgcccgcgca gccgaacgcg gagtttagaa tgcctgccgc cgtgcgggca
      481 atcggcgcgt cctccagcac cagcgcgtga ttttttgcct cgcagaggca cgaaacgcgc
      541 ttgccgttgg cggcggaggt ggcgtagatg tgcttaccga tcttggtgga tccaacaaac
      601 gtaacgcccg ccacgtcggg atgcgaaagc agcagttccg cttccactct ggagcaggta
      661 acgatattga ccacgccgtt tggcagaccc gcttcggtga ggagttccat gagccgcatg
      721 gaggtcatgg gcgtcatgct cgcggctttg agcacgagcg tgttgccggt ggcgatgcac
      781 attggcatca tccagcccat cggaatcatg gcggggaagt tgaacggcgc aataccgacg
      841 aatacgccca gcggttcgcg gtagagcgtg gaatcgaagc cggaggtgac gttcatcagc
      901 gactcgccca tcatcatgga aggcgcgccg atggcgactt cgaccggttc cttcactttg
      961 agcaggtcgc cgcgcgcttc gtcccagttc ttgccgtttt cgcggcagag gatatgggtc
     1021 agctcgtcga gatgtgcaat cagcaaatcg cggaagcggt agagcacttc tacgcgcttt
     1081 gcacacgggg tattcgacca ggcggggaag gcggcttctg ccgcggcgac ggcggcgttg
     1141 acctcgtcca ccgtgcagca gggcgtttcc gcaatggttt cgcccgtgct gggatcgtag
     1201 acgggcatgt actttgccgt tttgctttcg acgaacgcgc cgttgatata gggtttcagg
     1261 cgttggatat tcatggttta ttcctccgta aataggttct tgcgacagtt tattgcttta
     1321 ctggttcttt tttcgttcga tgacgcgtat ggccgcttgc gcaatgtttt ctgcccgcag
     1381 gccgaaatga tccatcagcc aatcctgcga gccgacctca ccgaaggatt ctttaattcc
     1441 gatgcgttcc atgggaacgg ggtgcgtttc gcccagtgcc tccgcaacgg cgctgcccag
     1501 g
//
